LOCUS       DAYYCY010000001        94381 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000001 DAYYCY010000000
VERSION     DAYYCY010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 94381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 94381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..94381
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.001"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(31..1503)
                     /locus_tag="ACHAZB_000001"
     CDS             complement(31..1503)
                     /locus_tag="ACHAZB_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFM8612718.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            1641..2114
                     /locus_tag="ACHAZB_000002"
     CDS             1641..2114
                     /locus_tag="ACHAZB_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM8612719.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(2267..2818)
                     /locus_tag="ACHAZB_000003"
     CDS             complement(2267..2818)
                     /locus_tag="ACHAZB_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM8612720.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            2923..3453
                     /locus_tag="ACHAZB_000004"
     CDS             2923..3453
                     /locus_tag="ACHAZB_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HFM8612721.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(3936..4379)
                     /locus_tag="ACHAZB_000005"
     CDS             complement(3936..4379)
                     /locus_tag="ACHAZB_000005"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8612722.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(4395..5555)
                     /locus_tag="ACHAZB_000006"
     CDS             complement(4395..5555)
                     /locus_tag="ACHAZB_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFM8612723.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKKQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            6062..7762
                     /locus_tag="ACHAZB_000007"
     CDS             6062..7762
                     /locus_tag="ACHAZB_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8612724.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQKKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(7864..8697)
                     /locus_tag="ACHAZB_000008"
     CDS             complement(7864..8697)
                     /locus_tag="ACHAZB_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFM8612725.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCQVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQSQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(9001..11166)
                     /locus_tag="ACHAZB_000009"
     CDS             complement(9001..11166)
                     /locus_tag="ACHAZB_000009"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIBC"
                     /protein_id="HFM8612726.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            11426..13720
                     /locus_tag="ACHAZB_000010"
     CDS             11426..13720
                     /locus_tag="ACHAZB_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HFM8612727.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGFPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            13713..14378
                     /gene="pgmB"
                     /locus_tag="ACHAZB_000011"
     CDS             13713..14378
                     /gene="pgmB"
                     /locus_tag="ACHAZB_000011"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFM8612728.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKCH
                     DHG"
     gene            14371..15378
                     /locus_tag="ACHAZB_000012"
     CDS             14371..15378
                     /locus_tag="ACHAZB_000012"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFM8612729.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            15400..16419
                     /locus_tag="ACHAZB_000013"
     CDS             15400..16419
                     /locus_tag="ACHAZB_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8612730.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDPVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(16700..18067)
                     /gene="nhaC"
                     /locus_tag="ACHAZB_000014"
     CDS             complement(16700..18067)
                     /gene="nhaC"
                     /locus_tag="ACHAZB_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFM8612731.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFVFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(18177..19598)
                     /gene="tyrP"
                     /locus_tag="ACHAZB_000015"
     CDS             complement(18177..19598)
                     /gene="tyrP"
                     /locus_tag="ACHAZB_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070908 - tyrosine:tyramine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-tyramine antiporter"
                     /protein_id="HFM8612732.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(19841..21718)
                     /gene="tdc"
                     /locus_tag="ACHAZB_000016"
     CDS             complement(19841..21718)
                     /gene="tdc"
                     /locus_tag="ACHAZB_000016"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFM8612733.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(22009..23265)
                     /gene="tyrS"
                     /locus_tag="ACHAZB_000017"
     CDS             complement(22009..23265)
                     /gene="tyrS"
                     /locus_tag="ACHAZB_000017"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFM8612734.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEVEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(23377..23582)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            23845..25146
                     /locus_tag="ACHAZB_000018"
     CDS             23845..25146
                     /locus_tag="ACHAZB_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFM8612735.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(25215..25814)
                     /locus_tag="ACHAZB_000019"
     CDS             complement(25215..25814)
                     /locus_tag="ACHAZB_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8612736.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            26068..26949
                     /locus_tag="ACHAZB_000020"
     CDS             26068..26949
                     /locus_tag="ACHAZB_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFM8612737.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTVVEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            26962..28572
                     /locus_tag="ACHAZB_000021"
     CDS             26962..28572
                     /locus_tag="ACHAZB_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8612738.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRMGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(28649..29308)
                     /locus_tag="ACHAZB_000022"
     CDS             complement(28649..29308)
                     /locus_tag="ACHAZB_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFM8612739.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(29591..30256)
                     /locus_tag="ACHAZB_000023"
     CDS             complement(29591..30256)
                     /locus_tag="ACHAZB_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HFM8612740.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(30403..31008)
                     /gene="dhaL"
                     /locus_tag="ACHAZB_000024"
     CDS             complement(30403..31008)
                     /gene="dhaL"
                     /locus_tag="ACHAZB_000024"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFM8612741.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(31036..32046)
                     /gene="dhaK"
                     /locus_tag="ACHAZB_000025"
     CDS             complement(31036..32046)
                     /gene="dhaK"
                     /locus_tag="ACHAZB_000025"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFM8612742.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(32021..32416)
                     /gene="dhaM"
                     /locus_tag="ACHAZB_000026"
     CDS             complement(32021..32416)
                     /gene="dhaM"
                     /locus_tag="ACHAZB_000026"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFM8612743.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            32570..32890
                     /locus_tag="ACHAZB_000027"
     CDS             32570..32890
                     /locus_tag="ACHAZB_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFM8612744.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(33002..33859)
                     /locus_tag="ACHAZB_000028"
     CDS             complement(33002..33859)
                     /locus_tag="ACHAZB_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFM8612745.1"
                     /translation="MKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTISSSG
                     AENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELEEALE
                     AAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQQAKE
                     FNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAASFQRI
                     KDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGVLVAA
                     AYGIIRKKK"
     gene            complement(33967..35270)
                     /locus_tag="ACHAZB_000029"
                     /pseudo
     CDS             complement(33967..35270)
                     /locus_tag="ACHAZB_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(35267..35776)
                     /locus_tag="ACHAZB_000030"
     CDS             complement(35267..35776)
                     /locus_tag="ACHAZB_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8612746.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(35951..38086)
                     /locus_tag="ACHAZB_000031"
     CDS             complement(35951..38086)
                     /locus_tag="ACHAZB_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFM8612747.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(38169..39824)
                     /locus_tag="ACHAZB_000032"
     CDS             complement(38169..39824)
                     /locus_tag="ACHAZB_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM8612748.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(39826..40140)
                     /locus_tag="ACHAZB_000033"
     CDS             complement(39826..40140)
                     /locus_tag="ACHAZB_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFM8612749.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            40310..40570
                     /locus_tag="ACHAZB_000034"
     CDS             40310..40570
                     /locus_tag="ACHAZB_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HFM8612750.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            40584..40898
                     /locus_tag="ACHAZB_000035"
     CDS             40584..40898
                     /locus_tag="ACHAZB_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HFM8612751.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(41628..42182)
                     /locus_tag="ACHAZB_000036"
     CDS             complement(41628..42182)
                     /locus_tag="ACHAZB_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFM8612752.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(42308..44236)
                     /locus_tag="ACHAZB_000037"
     CDS             complement(42308..44236)
                     /locus_tag="ACHAZB_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFM8612753.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            complement(44542..45258)
                     /locus_tag="ACHAZB_000038"
     CDS             complement(44542..45258)
                     /locus_tag="ACHAZB_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFM8612754.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(45259..47082)
                     /gene="glpO"
                     /locus_tag="ACHAZB_000039"
     CDS             complement(45259..47082)
                     /gene="glpO"
                     /locus_tag="ACHAZB_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HFM8612755.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(47096..48592)
                     /gene="glpK"
                     /locus_tag="ACHAZB_000040"
     CDS             complement(47096..48592)
                     /gene="glpK"
                     /locus_tag="ACHAZB_000040"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFM8612756.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(48728..50188)
                     /locus_tag="ACHAZB_000041"
     CDS             complement(48728..50188)
                     /locus_tag="ACHAZB_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8612757.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(50178..51473)
                     /locus_tag="ACHAZB_000042"
     CDS             complement(50178..51473)
                     /locus_tag="ACHAZB_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM8612758.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(51805..52482)
                     /locus_tag="ACHAZB_000043"
     CDS             complement(51805..52482)
                     /locus_tag="ACHAZB_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HFM8612759.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(52512..53603)
                     /locus_tag="ACHAZB_000044"
     CDS             complement(52512..53603)
                     /locus_tag="ACHAZB_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HFM8612760.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(53704..54138)
                     /locus_tag="ACHAZB_000045"
     CDS             complement(53704..54138)
                     /locus_tag="ACHAZB_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFM8612761.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(54722..56179)
                     /locus_tag="ACHAZB_000046"
     CDS             complement(54722..56179)
                     /locus_tag="ACHAZB_000046"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8612762.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(56176..58071)
                     /locus_tag="ACHAZB_000047"
     CDS             complement(56176..58071)
                     /locus_tag="ACHAZB_000047"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFM8612763.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPITLLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSPVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(58129..59904)
                     /locus_tag="ACHAZB_000048"
     CDS             complement(58129..59904)
                     /locus_tag="ACHAZB_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFM8612764.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(59954..61450)
                     /locus_tag="ACHAZB_000049"
     CDS             complement(59954..61450)
                     /locus_tag="ACHAZB_000049"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8612765.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(61644..63266)
                     /locus_tag="ACHAZB_000050"
     CDS             complement(61644..63266)
                     /locus_tag="ACHAZB_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFM8612766.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESINAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTVPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(63715..65028)
                     /locus_tag="ACHAZB_000051"
     CDS             complement(63715..65028)
                     /locus_tag="ACHAZB_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFM8612767.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGIAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(65273..65497)
                     /locus_tag="ACHAZB_000052"
     CDS             complement(65273..65497)
                     /locus_tag="ACHAZB_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8612768.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(65517..65939)
                     /locus_tag="ACHAZB_000053"
     CDS             complement(65517..65939)
                     /locus_tag="ACHAZB_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8612769.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(66083..66793)
                     /locus_tag="ACHAZB_000054"
     CDS             complement(66083..66793)
                     /locus_tag="ACHAZB_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HFM8612770.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(66811..67308)
                     /locus_tag="ACHAZB_000055"
     CDS             complement(66811..67308)
                     /locus_tag="ACHAZB_000055"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8612771.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(67345..67797)
                     /locus_tag="ACHAZB_000056"
     CDS             complement(67345..67797)
                     /locus_tag="ACHAZB_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM8612772.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(67932..68537)
                     /locus_tag="ACHAZB_000057"
     CDS             complement(67932..68537)
                     /locus_tag="ACHAZB_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFM8612773.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(68987..69607)
                     /locus_tag="ACHAZB_000058"
     CDS             complement(68987..69607)
                     /locus_tag="ACHAZB_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8612774.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLCQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(69609..70478)
                     /locus_tag="ACHAZB_000059"
     CDS             complement(69609..70478)
                     /locus_tag="ACHAZB_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFM8612775.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(70501..70908)
                     /locus_tag="ACHAZB_000060"
     CDS             complement(70501..70908)
                     /locus_tag="ACHAZB_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFM8612776.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(70927..72216)
                     /locus_tag="ACHAZB_000061"
     CDS             complement(70927..72216)
                     /locus_tag="ACHAZB_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM8612777.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(72243..72545)
                     /locus_tag="ACHAZB_000062"
     CDS             complement(72243..72545)
                     /locus_tag="ACHAZB_000062"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8612778.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(72570..74663)
                     /locus_tag="ACHAZB_000063"
     CDS             complement(72570..74663)
                     /locus_tag="ACHAZB_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM8612779.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKVFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(75152..75916)
                     /locus_tag="ACHAZB_000064"
     CDS             complement(75152..75916)
                     /locus_tag="ACHAZB_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HFM8612780.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(76027..76896)
                     /gene="mdcB"
                     /locus_tag="ACHAZB_000065"
     CDS             complement(76027..76896)
                     /gene="mdcB"
                     /locus_tag="ACHAZB_000065"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019361 -
                     2'-(5''-triphosphoribosyl)-3'-dephospho-CoA biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HFM8612781.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(76921..77997)
                     /locus_tag="ACHAZB_000066"
     CDS             complement(76921..77997)
                     /locus_tag="ACHAZB_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFM8612782.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(78021..78614)
                     /locus_tag="ACHAZB_000067"
     CDS             complement(78021..78614)
                     /locus_tag="ACHAZB_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HFM8612783.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(78618..80294)
                     /gene="mdcD"
                     /locus_tag="ACHAZB_000068"
     CDS             complement(78618..80294)
                     /gene="mdcD"
                     /locus_tag="ACHAZB_000068"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HFM8612784.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(80284..80583)
                     /locus_tag="ACHAZB_000069"
     CDS             complement(80284..80583)
                     /locus_tag="ACHAZB_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HFM8612785.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(80685..82331)
                     /gene="mdcA"
                     /locus_tag="ACHAZB_000070"
     CDS             complement(80685..82331)
                     /gene="mdcA"
                     /locus_tag="ACHAZB_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015451 - decarboxylation-driven active
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HFM8612786.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(82338..83249)
                     /locus_tag="ACHAZB_000071"
     CDS             complement(82338..83249)
                     /locus_tag="ACHAZB_000071"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFM8612787.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            83369..84226
                     /locus_tag="ACHAZB_000072"
     CDS             83369..84226
                     /locus_tag="ACHAZB_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM8612788.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(84413..84775)
                     /locus_tag="ACHAZB_000073"
     CDS             complement(84413..84775)
                     /locus_tag="ACHAZB_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFM8612789.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            complement(84964..85146)
                     /locus_tag="ACHAZB_000074"
     CDS             complement(84964..85146)
                     /locus_tag="ACHAZB_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8612790.1"
                     /translation="MIFFISEYINQTPFYSKNTEKINGGQVSTDFSIIVYFSGAFTDD
                     KNFIMNLKNDTTKIKK"
     gene            85121..86560
                     /locus_tag="ACHAZB_000075"
     CDS             85121..86560
                     /locus_tag="ACHAZB_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8612791.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(86567..89119)
                     /locus_tag="ACHAZB_000076"
     CDS             complement(86567..89119)
                     /locus_tag="ACHAZB_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HFM8612792.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMAFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(89233..90564)
                     /locus_tag="ACHAZB_000077"
     CDS             complement(89233..90564)
                     /locus_tag="ACHAZB_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8612793.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(90530..92008)
                     /locus_tag="ACHAZB_000078"
     CDS             complement(90530..92008)
                     /locus_tag="ACHAZB_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8612794.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITLLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(92313..94148)
                     /gene="tdc"
                     /locus_tag="ACHAZB_000079"
     CDS             complement(92313..94148)
                     /gene="tdc"
                     /locus_tag="ACHAZB_000079"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFM8612795.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
BASE COUNT        27407 a        19932 c        16358 g        30684 t
ORIGIN      
        1 caagcaagct gacaatcaaa actccaataa tcatgcatta tcccccgctt tcattagaac
       61 aggttctttt ttcttcaata aaaaggcaat aataaagcca agaatcccaa agatgatcgc
      121 tacgataaac gcggcatggt aacctgacag tgtggcattg attgcctgat ctttataagc
      181 taatggcgta ttatccaata cagatttgct tgggatattt gcttttgtca cattcgtcaa
      241 cacactgatc aaaatagctg taccaatcga gcttgcgatt tgacggaatg tattatttac
      301 cgctgtccca tgactgatca agttatttgg caatgcattc ataccagaag tcgttaccgg
      361 catcatgacc atggagatcc caaacatacg gatcgcataa aggatcacga tatataaaac
      421 tggagtcgtt tttgtcaaga aagcaaatgg tgcagttgcc gctgtcaaaa tgaacatccc
      481 tgtgatggct aaacgtttcg cgccgtattt atcgaaaatc gcacccgtca caggcatcat
      541 agcccccatg atcaatgcac ccggcaacaa catcaagcca gaatggaatg ctgattctcc
      601 acgaatattt tgaatataca atggtacgac catttctgcc cctaccatcg ccatatttgt
      661 tacaccagct aaaacagctg caatcgtgaa tgtagatgat ttgaatacac gaagttccaa
      721 gaatggatgt tccaaatgta attgacgcca tgagaacaag gcgatgaaaa tcacaccaac
      781 aactaagaaa ccaattactt tcaagcttcc ccagccgtca tctcctacac tagaaaatcc
      841 gtaaagtaaa ctaccgaatc caatcgttga taaaatagcg gataaaatat caatgctagg
      901 atttgacaat ggaataacac ttttcattag gaagaatgct aaaaccaaga caagaacaac
      961 gattggcagt accataccaa acaagtcacg ccaatggtag ttatcaatga tataaccaga
     1021 taatgtaggg cctaaagcag gagcaagtcc aataacgatc cctgacatcc ccattgctga
     1081 tcctcttttt tctggcggga aaatagatag catgatattt tgcaataatg gcatcgatac
     1141 tcccacgccg caagcttgga tcaagcgacc gattagtaat gtactaaaat taggtgctgt
     1201 aaagcaagta atcgttccga tcaaaaagat cgtcatggca gaaagatata aatttcttga
     1261 actgaattta ttgatcagcc atgctgttat tgggatcatg atcccgttga ctagcaagaa
     1321 accagtcgtt aaccattgca catcagacgc tgaaatatcg aaagctttca tcaatgtagg
     1381 aaaagctgtc gtcaataagg tctgattcaa aacggtacaa aatgtaccaa ttaataagac
     1441 aacaactaaa agtgtccgat tatatggttt tccgtaaata tctacgggtt ggttgttact
     1501 catatttctc cttctttctg aattttttct gaaactacta gaatacttta gtcctaaatt
     1561 cgctatttgt caacctactt gacacataag ttaatacgga tataattaag ggaagcaaga
     1621 ggacaaaagg agtgacaaaa atggttgggg cttcccttaa acgtttgttg gaaaatgatc
     1681 aattggttgt tggcatcagt gaattaagtg atatcgtagg tgtgtctcca cgtcaattaa
     1741 ggtattggga acaaaaggga ttcattcgtt cgatcgaagc tgatgcgaat tgtcctagaa
     1801 aatatcgttt acctacagtc ataaaagtag aactgatcaa aaaatattta gatgacggat
     1861 ttactttgtc aaaagctgca gaaaaggcag aaatccgcca aaagaaaatg caccatgtcc
     1921 gtaaagtttt ttcaaaagca atcaaagata tcgaattgat cgacgaacgc tatacgattt
     1981 tacaaatcgg taacttcaaa gaagagaatg ctaagatgta catcattcat gacaacgaga
     2041 cagacgaatt aacttacaag ctccttcctg tcgatgaaaa agctgactac gaacagttgt
     2101 taaaagaact ctaaacgcca ctaatctcct aagtaccaag ctttttaata agtagatgct
     2161 acataaatac ttgaaaaaag gcctgtttga acataagaat atccgaacgg tgatctgcgt
     2221 ttctttcata tagaaaggaa ttctccatca ctgttcggat atttttttat ttttttcggt
     2281 tactttcttc taatacatct gttagccaat ttgcatattc tgtttcccga ttgatcgcat
     2341 gcgctaaaat caggtagtgt ccgtaatttt catgtttttc tttttcagta ggaaagatcg
     2401 ttttttttcg ctctaataga tgaagtcgtt tttcttcatg aaggtcacgc tgctgctgaa
     2461 taatttctac taatgccgga tcattcacgt ctttgacgaa atacaatttc agaacaaatt
     2521 cgtctcgatt aaccggtagt tcattggttg gtgtatgaat ccactctgcc aattgttcct
     2581 tcccttcttc tgttaattca taaactttct tttccaattt ttcacctgtg atctctactt
     2641 gatgctgaat gattccttgc tcttccattt ttgcaagttc aggataaatc tgactatgtt
     2701 ttgcttgcca aaattctcct atttcatttt tgaacgattg attcaattca tatcctgtca
     2761 tcttcttctt acttaataac cctaacaaaa tatatcgaag tgtatttttt cttgccatca
     2821 gttttccctc cttgacaaat ctttaaatcc attctatcat aaatgagtaa tcgaaaacat
     2881 gtagtttatt acatgtttta atttataagg agatgcttaa acatgaaaga atttaaagaa
     2941 ttggaagatt ttattggaac tcactttatc tatacgtatg acaatggttg ggaatatgaa
     3001 tggtatgcca aaaatgacca tactgttgat taccgtatcc atggcggaat ggtcaaaggc
     3061 cgttgggtaa aagaccaaga agctgatatc gttaaattga cggacggcgt attcaaaatc
     3121 acttggacag agccaacagg gacagacgtg gcattggact ttatgccaaa tgaaaagaaa
     3181 cttcacggaa tgatcttttt ccctaaatgg gttgaagaac atccagaaat cactgttact
     3241 ttccaaaacg aacatatcgc agaaatggaa gcagctcgtg aaaaatatga aacttatcca
     3301 aaattacttg ttcctgaatt tgctacgatc acttatatgg gtgatgcagg tatagacaat
     3361 gaagatgtta ttagcgaaga accatacgat ggaatgccag atgatatccg tagcggaaaa
     3421 tacttcgatg aaaattataa acgagtaaaa taaggttgtg aaagagaggt tgtgaaatca
     3481 gcgttctgac ctgagtatta gagaaagaaa cggaaaaata gttcctcatg ttttgccgtt
     3541 ttttgagcta ataccgaggg tcagctgatt gaacaccgtt tatcaagagg ttgtgaaatc
     3601 agcgttatgc tttgagtaat aagaacgcta gttagaaaat agcttttcat attttctagg
     3661 ctagtgggct tattaccgag aagcagccgt ttgaacaccg tttatcaaga ggttgtgaaa
     3721 tcagcgtttt gacctgagta ttagagaaac aaatggaaaa atagttcctc atattttgcc
     3781 gttttttgag ctaataccga gggtcagctg attgaacacc gtttatcaaa gattgtaaca
     3841 actgcatatt cacacagtta aattagtgac ttcaaaatga aataatgctc ccagcaaaaa
     3901 attaggctgg gagcattatt tcgttattct gttcatcaaa aatcagcgtt ttgattccat
     3961 ttcatcacaa gttcctcctc atttactgct tcatcttttt gctgtgtttg aattgtaaaa
     4021 ccttgtgaaa gatagaaatg aatagctgat tgattttttt gatagactgt caaagtcagt
     4081 gttttattgg tttcttttat ttttttcatt agttgcgtac cgattctttg ttgccggtat
     4141 tcccttttca caaagattcc tgcgataaac tgatcaatca ccccagcaaa accaataatt
     4201 tcttgatcta tttctgctaa gtaaatcact gcatctggta aagctttcct aacagattct
     4261 acttgattct cccaataagt tgatggaatg aattcatgag aatccaagtt agcatgcagc
     4321 caaatcgata gaacagattc cagttctttg gcagacaatt ttgttgttgt gcggatcatt
     4381 ttttctctcc ttacttattc attgatttta aaataaaaac catttttttg aagctctttt
     4441 acaagttttc gcccctcaaa aaagtatatc ttctcttgtt ttacttttct aataccaaaa
     4501 gaaacagcaa ctttaggatc atcagctgaa acgattcgat aagttccacc aggcaattga
     4561 tccagcttga ctttcccatg atggtcgctt tgtactgaaa acggcacacc atttctagta
     4621 agtgtttttt tcttatgttt caaaatgaat gaactaccat ttaccggttt ccctgacgaa
     4681 tcactaataa taaatgaaaa atcaagcact tttttactta atctatactg atagattagt
     4741 cgtccgataa acactaacag tatgactaca gctattatcg ttcctgcaag aataaaccat
     4801 tgccgcatgg ttctttcctg atttcctttt ccttcttctt tttttaccga ttcgttataa
     4861 ggaatccgat gtcctgtcac tagcaaacga tgcgtgttga tcatatacgg cgtacatgta
     4921 agcaaggtaa ctaagtcttc tccttctgaa atcgttagaa cagacgtgtc atctggcagt
     4981 accacttgta tatttttaac ttgataagcc aatttctccc ccaaaacagt caaaataaac
     5041 gtgtctccgt tttctaaacg atctaattcg gtaaataacc gtcgattagg taaaccgctg
     5101 tgagcagaga tcactgaatg cgtcgaattt ccaccaagcg ggaaagaggt tccttgcaag
     5161 acgcctgctc cattttcaag gatcctacca ttgagccgat cataaagtga aatatttaat
     5221 tggatcttag gtatattgat acttccgatc agccattcct ctaattggct ggatgttgta
     5281 ctttcatttc tgttcttttc attgaaagga tcagtaccag gattgaatcc ttggtcagtt
     5341 aatcgttgtt ttttctcctc cattttcttt cgctgcactt caatttcagc tgcggtccgt
     5401 ttttgtactt gttcgatacg tttttgatca ataaaactat ttaaagaatc cacataaaat
     5461 ggatataaag cgatcagtaa ccctacgacg atacatatta ataaaaacaa acgaaacaaa
     5521 agtcgacgag actgtttttg cttactctgc ttcacatgtg tcctcccttg caagtttttt
     5581 tgttctttct tttattatat gggttcagga aaaaaataga aaatgaaagc cgatgaggtt
     5641 gtgaaaaagc tgtcctgcat caagtaataa gtacagccca aaaaaatagc tactcatatt
     5701 ttttgttggg ttgggcttat taccgcagat gcaagctttt gaacaccgtt taaggaagag
     5761 gttgtgaaaa ctgcgttatg ctttgagtaa taagagcgct agtcagaaaa cagcttctca
     5821 tattttctag gctagtgagc ttattaccga gaagcagctg attgaacacc gtttaccgag
     5881 aggtcatgaa aaagctactt tgtttcgagt attaagaaaa attctgtttc aaaacaagga
     5941 gatgcaacaa aacttatgtc gcatcctctc tccttagaac tttcacttat gtttttcttg
     6001 taaaattcat aaatattttc atgaattttc taatttcttg cctacctctc gcataggata
     6061 tatgatgaat ttatctaaag tttgcaaagg agttcgacaa ttgaaaaaat atggcccatt
     6121 tttagcaatt tttttcattg ctttggcatt tctcatgcct catttattca cacataaact
     6181 aattttgggt gctgatgccc cattccatta caatcggttc tatgatgccg cgcaacaaat
     6241 taaatatggg gatttccatt attttccttc cctttatgga ttccagcaaa gtggcaggat
     6301 cgtgaatgcc gtttacggcc catttttcgc ttatttacaa ggattgatcg tgttgcttgc
     6361 aggtagctgg tttcattatc agattctttc aaatttcatt ctttatctta tttcaggatt
     6421 ttctatgctg attttactga gatatgtcaa tgttcgaaat tgggtaagtg taccagtagc
     6481 cgttttattc atgagtacgt attctattca atactggtct atcaaccaag gattcactag
     6541 ctgggggact tgtttttatc ctttatgtat gatccctatt gctgatttcg tccttcataa
     6601 aaagttccct gttatcaaag tcgctgtctc agtgggactt atgacacaga tccatttact
     6661 gtcttcgatc atgttagctt tgatgtatat ccctttttat ctttattact tttttaacca
     6721 gactgaaaaa ggaaaagcac tgcttcaact gattaagtcg gtcttgcttt attttgttct
     6781 gacagcaaat atttgggcag gattagctat tgtttacagt gggaataaga ttcttcctcc
     6841 ctttgttaac agagaaatga gtcaaaaggc gatagatctg gatggcagtt attggctcca
     6901 ttaccctaag gcctttccta tattgatatt atcaggtttt ttgcttttta ttttctttca
     6961 acaaaagaac accttattcc aaaaaatcgt gttgtacaca agtcttgttt tcttttttct
     7021 ttctactagc ttgattccat ggacgacatt gatcgaaaaa aatgtgcctt ttattagttt
     7081 gatccagttt ccatttcgat ttttcgtgcc atttacgcta ttgcttctac tttaccttgc
     7141 cctttcactg aaccagtggt ctgaacaaaa gtggttccgt cttttaagta tatttggcat
     7201 tcttattttt tcagcacaga cgatccagaa cttataccag catctggaaa agtgggaaaa
     7261 tgaaacattt gtcagtcgac atacttatct attcgatacg ccggaagaag caaggaaaac
     7321 attcttcagc aaagatatgt ctaccagctt gttcgttttc caaaaaacca cacctgacta
     7381 tctgccaatc tataaggaga caaaagacaa taaatacgac cggtataacg aagaaatact
     7441 agaaaacgaa acaactttta taaaaacaca tccaggcggt cttttgacaa tcaagtggaa
     7501 aaacactggg gacaaaactg ttcatatccc tgttatcgtc tacgatcgga ctgtgctaca
     7561 gcaaaatgga aaaacattga ctgattatga agtaacagat atcgggaccc caatcgtgaa
     7621 gcagaaaaaa ggaatcaacg agctcacgct tcattatcaa acgccaatct atttctattt
     7681 cattctttct cttactttga ttggctggtt taccttgcta tgtttattta tttatcatag
     7741 atacaagctt ttacgtgctt aggcaaaagg gataaataca tccgatcaaa aaactctcca
     7801 aacggtataa tcgtttggag agttttattt taatcagttt ttttatgtta tatgttatat
     7861 tttttattcg ttcacatcta attcgatttc caccccgtag tgatcactga tgattggact
     7921 atcgatccca tcaaaaatcc gttgatattt tttaacctgc attccttcag ggacgaaaat
     7981 aaaatctata cgcaattttt ctgtattttg ttcccaacca tctattttct tttccacagt
     8041 agctgctttt cctttttcat tcgcgacaac atacgaatct tgaagaagaa ggcaactttc
     8101 tcttacagta taataacctt gagagtctac tggattattg aaatcaccta ggaaaaaaag
     8161 tggtacgcga gtttccaaaa agtagttttc caagtttttc cactcaaaat aaaagccact
     8221 ttttttatct gtccaccaag aaaagtggca gctcgagaca aaaatgtcct gatcatctat
     8281 ctttgttttc ccaatcaaga tcttgcgtgt ctgataatct tctttctttt gagattggga
     8341 gacggtaagg acctgacttt ccaatgtttt tttcgtaagc agtgcatttc cttcttcata
     8401 tttatcataa ccgatgtggc tcatttccca actccaaaaa tactcttttc cgtgttcctt
     8461 taaaagctga actaaacggt aggcaaaatt atctttacgg ataggtactt gattctttac
     8521 ctgacagaac ttcattagct tcgcaggttc tacttcttca gattccagta attggttgat
     8581 ttcttgtaaa gcaatcactt cataatcttc taacaggatc ttatctgcta attcttgcaa
     8641 ttttttctct ggttctggct caagccagct atgcgtgttg agtgtcatga atttcattgg
     8701 ttttctcctt gtcttcattt cgaaacaaaa gggttgtgac aaaatttttg tcacaacctc
     8761 tccagcaaat agtgttctcc tgctaactcc tcagtaataa gctcaatgga ccaggagcta
     8821 ttttttgttt ggctgttttt atcacttggt gcagggcagc tttttcacga cctctccact
     8881 aaacagtatt caaactgctg cttctcggta ataagcccaa ccaaacgaaa aataggagga
     8941 gctatttttg tttggctgtt cttatgactt gatgcaggac agctttttca caacctctcc
     9001 ttacatttta atggagccga tcactttacc agctgtcact gtacctaatt gcttgatcac
     9061 tacttcttct actttatcgt ttgtaaatac gatcatgatc gttggatctt tttcttcgtt
     9121 ttggatcaca tcaagatcca tctcagctaa tttcgttcct gttcctactt cttggccttc
     9181 tttgacagca atctcaaaag ctggctgatt catttggact gtatcgattc ccatatggat
     9241 caaaacttcg ataccatctt tcgtttccag cccaattgca tgtttgctag ggaaaatact
     9301 tgtcactttt ccagcgatcg gcgcaacaac ttctcttgat gttggaataa cagcaaagcc
     9361 gtcacccatc attttttgtg agaataccgg atcttttact tcagataaag gaatcacttc
     9421 tccagaagct gcggaaacta gttcttgttc tatacctaag tattgcttac tacctgcttg
     9481 tccatttcct gtagaaacca tttctggctc tggaatctct gctcctgatt gcagcagatc
     9541 ttcgatatct gatttcaaga catctgcttt tggtccataa actgcttgga ctccattgtc
     9601 tttgacgatc aatcccatcg cccctgctcg tttccaggct tcttcagtcc ctactttttc
     9661 tcgatcattt acacttacgc gtaaccgagt catacaagca tcgacatctt tgatattttc
     9721 ttttcctccc agcaaatgaa tgatctggac cacttgctga tctgctgttc ctgttccgct
     9781 gttagataca acggtttctt cactgtcatt gtcgtaattt ccgttacgtc ccggtgtagc
     9841 aaagttgaat tttttgatca agaaatttgc taagaaatac gtaacaacac caaaagcgat
     9901 cacacacaac acgaagttga taagatctcc acctagaccc gctttgatag ctaacggtgt
     9961 ccgagtcagc aattcgatat tcccaaatga atggacccgc aatggcaaga tatctgccat
    10021 cgcaaatgca gccccttgga tcactgaata aatgacatat aatggcacag cagcaaacat
    10081 aaacatgaac tccaatggtt ctgttacacc tgttagaaag acagccaacg ctgctgaaaa
    10141 atacattgat ttatatcttg ctttcttatc aggatcgaca ttacgataca tcgcaaaagc
    10201 cattcccatc aagatacctg aagaaccaat catttgtcct actttgaaac gagccggcgt
    10261 ccagttatgc aacacaaact catattgcga catatcccct gcacctttaa gattcactaa
    10321 atcagtggcc caagccaacc ataacggatc ttgcccaaat acttgtgtcc ccgcctgtgc
    10381 gcctgataaa atctcatacg atccgccaag ctgtgtatag ttgataggaa tcgtcagcat
    10441 atgatgcaat ccaaatggca atagtaaacg ttctaatgtt ccaaataaaa acggtgctag
    10501 aatcggtgcg ctttcttgtg attctgcaat ccataaacca aaattattga ttccagcctg
    10561 tacgtttggc cacaccacag caagaatcaa tgccacgatt gttgaccata agataacaac
    10621 aaacggaaca aagcgctttc cattgaaaaa tgacaaagca tccggcaatt ttctataatt
    10681 atagtactta ttataagcca ttgccccgac aaatccagcg atgatcccta cgaatactcc
    10741 catattcaaa gctggtgctt ccaatacact tgtaaaaaat cctttaacca tgatcttcgt
    10801 accgaacaga gtgtgggtaa atgcatcttc attcgcaagc atgtcacttg tgacaccaaa
    10861 gatagcccca gtgatccgat tgattaaaat aaatgagatc ccagctgcaa atgctccgcc
    10921 tgctctttct tttgcccaac ttcctcctat tgctaaagca aataaaatat gaagattacc
    10981 aataattgcc caaccaatat tttcaattac tccgcccgtt gtgacaagcg gcgtccaatc
    11041 aggattgatc aagggaattg atttccctat actgatcatc aacccagctg ccggcataac
    11101 tgctaccaca accattaaag cctttccaaa tttttgccaa aactcaaaac taaatatctt
    11161 tttcattccg tttagctcct ttacgcaatc ggtttcgtta aacgtattat agcgtgcgtt
    11221 ttcttttttg taaatgcttt attttttatt tttctgtcta taccattcta taaaacctat
    11281 aatacgtgtg ttcccttcta taattttctc ctaaaaattt atttttttaa gcttttttct
    11341 atgaaatatt ttcttttgtc tattgacttg tgcaatcggt tgcgtttatt atgaacgtat
    11401 aaaacagctc attaggagga gaaaaatgaa acagatcaaa cgactatttg ctattgatcc
    11461 atggaaaatc agcacgacac aattagataa aaaaaatctt cgactgcaag aatctttaac
    11521 aagtattgga aacggttata tggggatgcg cgggaacttt gaagaaactt actcagggga
    11581 ccaccatcaa ggtacttatt tggcgggtgt ctggtatccc gataagacac gtgtcggctg
    11641 gtggaaaaat ggttatccag aatactttgg aaaagtgatc aacgccttga actttatcgc
    11701 gatagatatt tcgatcaacg atcagctgat cgacttggct tctttggaac cagaagactt
    11761 ttactgggaa ttagatatga aaaatggtgt attatctcgt acattcacaa tcactacttc
    11821 tactaataaa gtacgcattt ccttcgaacg ttttctgagc attgtcaaaa aagaatcagc
    11881 ttatatctat ttaactatag aaatgcttga aggtagcgga acagtcaaac tcgtatcaaa
    11941 attggatgga aatgttcaaa acgaagacag caactatgat gaacattttt ggaatgagat
    12001 tgatcgtgga actatagaag aaatcggcta tttgacgact aaaactattc caaacaactt
    12061 tgggattgaa caatttactg ttactgcagc aatgcgccat ttcgttaatg gaaaatcagt
    12121 catccctgac tatcacgaag atactttagc attacatggt tgcttgtctg cagaattatc
    12181 tcaaggtgag acatggtcag tggaaaaaga aattatcgtt cttactagta gagatattcc
    12241 agaagcgcag caagcagaaa aagctgtctc agaattaacg aaccagacaa acaattatat
    12301 ccaagcaaaa gaagagcaaa cagcggcttg ggcagaacga tggaagcttg ccgatgtggt
    12361 catcgaagga gacgaagaag cacaacaagg tattcgattc aatttgttcc aactattctc
    12421 tacttactac ggcgaagatg aacgattgaa tatcggcccc aaaggattca caggagaaaa
    12481 atacggcgga gccacttact gggatacaga agcgtatgct gtccctcttt atcttgcttt
    12541 agccaaacct gaagtaacta agaatctgct gaaatatcgg cataatcaac tgccgcaagc
    12601 aatccacaac gctcagcaac aaggattaaa aggcgcgctt tatcctatgg tcaccttcac
    12661 tggtgtcgaa tgccataacg agtgggaaat cacattcgag gagattcacc gaaatggcgc
    12721 gattgcctat gcaatctaca attatacgaa ttacaccggc gatacggaat atttgaaaaa
    12781 tgaaggactg gaagttttag cagaaatcgc acgcttctgg gcagaccgtg tccatttttc
    12841 aaaacgagca aataaataca tgatccacgg tgtgaccggc ccaaacgaat atgagaacaa
    12901 tatcaataac aattggtata caaatacgat cgcagcttgg gtcttgcgtt acacgcgaga
    12961 aaattattta acttatcaaa aagaagctgc tatcactatc agcgaagaag aactagccaa
    13021 atggaaagat atcgcagaga acatgtactt cccagttgac gaagaattag gaatctttgt
    13081 ccagcatgac acattcttag ataaagatct catgcctgta tccgatattc ccacaagcga
    13141 tcttcctctg aatcagcatt ggtcttggga caaaatcctt agatcttgct tcatcaagca
    13201 ggcagatgta ctacagggga tttatttctt caatgatact tttacaaaag aagaaaagca
    13261 aaaaaacttt gatttctatg aaccaatgac tgtccatgag tcttcccttt cgccaagtat
    13321 ccattcgatt ttagcagcag aattaggaat ggaaagcaaa gctgtagaaa tgtaccagcg
    13381 aacagctcgc ttggatttgg ataattacaa taacgacaca gaagacggct tgcacatcac
    13441 ttccatgact ggtagctggt tgtcgattgt ccaaggattt gctcaaatga aaactgccca
    13501 tgatcagttg agattcgcac catttctgcc aaaaacttgg aaagcttatt ctttccatat
    13561 caattatcgc ggacgtttat tgttcatctc agtcacagat gaacaagtta ctttcgagtt
    13621 attagaaggt tttcctctag aaatttacgt ctatgaacag tcttattctt tagcggatcg
    13681 tctagaaata acgctaagaa aggaggaagc aaatgtttaa aggggtattg tttgatttag
    13741 acggtgtcat taccgatact gctgaattcc attatcgtgc ttggcgaaag cttggtcagg
    13801 aaatcggcat tacgatcgac cacacattca acgaacagct aaaaggcgtc agccgagaag
    13861 actcactctc tttgctttta gcttatggcg gaaaagaaca ctctttttca aaggaagaat
    13921 ttgcagaatt agcgaaaaga aaaaacgatt attatttaga gatgatccag acgatcgaac
    13981 caaaagatgt cttccccggg gtcgtgcctt tactggattc tttaaaagaa gaaaaaatca
    14041 agattgctct tgcttctgca agtaaaaacg ggccattcct acttgaaaaa atgggtctta
    14101 caccttactt tgatgctatt gccgacccgg cagaagcaac aaacggaaaa cctgcgccag
    14161 atatcttcct ccttgcagca aaagctgtcg gactaaaagc agaagaatgt atcgggatcg
    14221 aagacgcgca agccggtatc caagccatcc tctcaagtgg cgctcaacca attggcgtgg
    14281 gacgtgctga agaccttgga gaggagatcc cgcttgtccc tgatacgact tttcttacga
    14341 tcgattactt gaaagaaaag tgtcatgacc atggataatc cagagatctc cgtcgtacca
    14401 gttccagaga ctgaaataaa gaaaatcaca ttgaagaacg actacttaga agtcgttctt
    14461 ctcaattacg gtgcccgcct gcaccaaatc ttcgcaccag acaaagaagg aaaatcagaa
    14521 aatgttttat tgtcttacga tcgtttcgaa gatgttctaa cagacaaaag cttttttgga
    14581 gcaactgtcg gacctgtagc cggaaggatc cgggatgctt cttggggtga gcatagattg
    14641 gagaaaaact gcggatcaca ccatatccac ggtggcacga aaggctggtc tttccaattt
    14701 tgggaagtag aagttttcaa aactccatca tcaataggtg tggtctttta cttaaaagat
    14761 gaattttcta cctatccagg accaatcact gctacgatca cttaccgttt aacaaaaaac
    14821 gaattagaga tgacaacctc tgctagtagc cttgttgaaa ccatctgcaa tccgaccaac
    14881 catgcctact tcaacctttc aggaaatgga acaagagaca tctatgaaca tcagttaacg
    14941 gtctttttag atggtgtgct tgaattagat caagaaaaac tgcctactgg aaattggaca
    15001 aaaaaagaaa aactgccaat cgactttaga aaatccccaa cactgcaaga gatccttgca
    15061 tgttatccag atggactaga tgatgtattt cttttgcatc atcctaggct ttccaaaact
    15121 tccttacaat tattcgagaa gcattctggt cgccaaatga ctatagcaac ttctaataaa
    15181 agtatggtat tattttctac gactggattc gaagctgatt tctctgtcaa tggaaaacag
    15241 atgcattcaa actacggatt agcaatcgaa ccgcaagaaa tccctgatat cgttcatttc
    15301 cccaaatttg gatcgatcaa cctccatcct ggtcaagaac ggatcagtca gacgatttat
    15361 cggttctctg cacaataaca agagaaaggt gtttttatca tggctattac cgtaaaagat
    15421 gtggcgaaaa aagccggtgt tgccacctcg accgtctctc gtgtcatcaa cgatcatcct
    15481 agtatttccg agacaacaaa gaagaaagtc cgaaaaatta tggatgaact aggttatgtt
    15541 cctaacatga ctgcccgcaa tctaggaaaa cgcatttcca gtgccatcgg cgtaatctta
    15601 ccccctcttg attcaaaaga gcggatcggt aacccatttt atcttgagat catggaagca
    15661 atcaacgaag aagcgcggaa ttatgatatg actacagcaa tcgctactgc taaaaatttt
    15721 gaattgctgt tagagaacgt ccagcgtatg catttacaaa aacaagtaga tggttttatt
    15781 ctagtgtatt cagatagtca agatcctgtc attgattatt tataccaaaa ccacatccct
    15841 ttcactttga tcggacagcc ttatcatcat gagaacgaaa tcgtttatgt agataatgat
    15901 aaccagcttc taggtaaaca agcaacagaa tttttgatcc agaatggaca taagaatatc
    15961 ctgtttgtga caaacacgac tcatgaaagt ttgtatttcg aacgatattt cggttatcaa
    16021 aaagcaatga tgatggctgg tcttcctgcg ttatccagtg tagtactaga gcatccggag
    16081 gattatctgt cctttgagaa aacgttgaaa gaaaacgaag ccacagcatt ggtggtcatt
    16141 gacgatatct tcgcccttcg cacgatgcag ttggcgcaaa tgtacggcta tgtcattcca
    16201 gagacactct ccgtcattag cttcaacaac tcgatcttct caacattaac acacccttac
    16261 cttacaagta tcgatatcga tatctccgaa ttaggacgtg tcgctatgca aaagctgcgc
    16321 gaattgatcc atgaagaatt gagcagtggc gtacgtttgg tcatccctca tcgcttgatc
    16381 aaaagagaga cagtcgttcc tttagaaaac ccatactaaa tagctgactc actaaatacg
    16441 ctgaccattt atcctatggt cagcttttta tgtaaaagaa gatactactt ctaacacatt
    16501 gtttatctgt aaaaaagagc gtgggacaaa agtaaagaac acttttgtct cacgctctaa
    16561 acacgtataa acggcggaag cagaagcaac tccttcggaa ataagctgaa attcacaaaa
    16621 atttaaaaag caattttctt gagtttcttc ttatttcttg gagttaaacg cttctgtccc
    16681 agcctctttt ttccttcctt caatttttta cacgtcgacg attcaataaa aaaccgccta
    16741 atattgtaaa gacaggtgca agaatcgaga aaaagacgaa tggcaagtaa tgcaacgcgc
    16801 taactttcat cgttcccata atgaatgtgc cgctgactcc ccacggtacg agagaattga
    16861 cggtcgcacc tgaatccgct agtgttcgtg tcaaataggt ttttgataag ccaacgtcat
    16921 caaaagaacg cttgaatgtt tctcctggaa gaatgatgga cagatactgt tctccaacga
    16981 tcagattgat tccgatacag ctcaaggcag taaacccgac taaacgagaa ggtcggtcca
    17041 tcttttcttt taattcttga acgatggtat caacgatcag atatttgatc agtaacccac
    17101 ctaatgccaa tgcaagtaaa atcaaactag cagaacctaa catactcatg atcccgcctc
    17161 gagaaagcag ttcatctagc tttggatcag gtgtatttgc gacataacca ttcatcaaga
    17221 tgtctgctgc ttttccgacc gtcaaacttt tctcatggat aaaagaaaga acgagagcag
    17281 ctgtcgatcc cgctagtaat gtagggatag ccggaatttt tttgattgcc cccccaaata
    17341 aaataactag tggaatcaat gtccaaggag agatccaaaa gctcgctttc atgttttgga
    17401 tcatttcatt gatactagct agatcggctg aatgactgtt atgtcctaat atcgtataaa
    17461 aaatcaacga aaggacgaat gctgggaaat ccgtataacg cataccagcg atatgttcga
    17521 atagattaac tcctccaata cccgctgcca agttggtcgt atctgataaa ggcgagcagt
    17581 tattccctaa aaaggcaccg gaaacgactg cgcctgtagt cattgcatca ttgaatccaa
    17641 gtaaatgacc gatccccaaa aaagcaattc caatcgtcga aatcgttgta aatgaactgc
    17701 cgacagtcac tccgacaatt gcacaaacaa gaaatgctgt cggtaaaaag aaacgcacag
    17761 aaacaagttt aaatccataa accataatcg tagggatggt tccagataaa atccatgaag
    17821 caactaagac accaatcaaa agaaaaatca ggattggaat gatccctgga gtgattcctt
    17881 tgatgatccc gtcatggatc tcatcccatg tgaacttttt aaatctgcca taaaacaaca
    17941 aacacatgaa agctgcaagg atcgggatat gtggcgagag ctggaatccg atgatcagta
    18001 ctcctaggag tgccaacaac acgatcaata cagcgatact ttcttttaaa gaaatatttt
    18061 tattcatcgt cgatctccta tttcaaatga aaaggccctg ccatgaaaaa actattcact
    18121 tggcaggacc tcctactaaa tcagcgacaa aacgtcggct gaaagactta ttttttttat
    18181 ttttcttcta aacttttttc tacctcaatc ccccaagaat ctttcttgaa gtgattgatg
    18241 atcaatggaa taacgaacat gaccactgtg atagctgcta ctaataatgc atcactcaat
    18301 gtcgctgtac ctaatgtagc aaatacgaca acgatgatag agaagatcag catgcagctg
    18361 actacccaag ctaatccgtt tccgttttta ccaattcggt aaggacgttc gaattctgga
    18421 tgtttcttac gcattttaag caatgaaaca gcaatcaaaa tgtacacgat acaataaata
    18481 acagttgtag catttgtaag tgtcaagaat acagcactta cgtcatccat tacaccataa
    18541 agcaaagcaa ataatgaaat aacgattgat tgtgttagaa cgacatttct agatacgccg
    18601 tatttgtttt ctttatggaa tccgaatttt ggtggaagga atccttcacg agctacttgg
    18661 atgattgttt ttgatggtcc agtaacccaa gctgataatt gtaaaagcac accaataaat
    18721 accatgaagc tgaagatatt tccgatcact tctggtaagc ctaggatttg acagtcaatc
    18781 aagataggct gcgtgatatt tgctaattcc atttttccat ctggtacgac atttgctaca
    18841 agcatcgcat tgatgacatt caataaaact aatccgatca aagcgataaa tacgcctttt
    18901 gtataatttt ttgttgcgtc tttcaaacga ggcatgtaaa cagaactcat ttcgatccca
    18961 acaaaaataa atgtgatacc agctaagtat ttcaatgaat caatatgttc caagtcaggg
    19021 aaagcttttg accatgaaaa gtcacctaga tagccgtttg agactagtcc gactttgaag
    19081 gctgccaaaa cacctaaaac gaacatgatg actactggga tatatacccc tagccataca
    19141 ccgatgttcc cgccgacttt tgccatatcg aatttcaagt tcaagatcgt gatgatccag
    19201 tagataacta aaatacaacc taaaacgaac cagtgattat tgccaagttc tacattacca
    19261 aatgtgtttc ctaacaatgg tcctaaagta gaagcgacca ttaccatacc tgggaacatt
    19321 tgcacccata atagccatgc aacgacaaat ccccatttgc tgcccagtgc tgtttttacc
    19381 cataactgag gaccgccttc ttgaggaagc atcgtggaaa gctcaccgga aaccattgat
    19441 atcggaccgg caaaaaacaa aactgcaaaa attgaataaa aaatcaatgt ccaaccgact
    19501 gcggctaacg taggaatact ccgaaccgtt gcacacagtg ccattgtcag accgataaag
    19561 gtcccaagac ccattttact tacttcagat ttactcaccg ttgacactct ccttgatttt
    19621 ttggataaaa taacggtgtc tttgaagtag tttcagcaat aaacgaaaca attcagcgaa
    19681 tatttgaggg gattctttga gtctattgct tgaagggttt cttcaaagac aacctcaaaa
    19741 caaagggatc gaagagctta ggtctcccct tgctcttcag atccaaacat gcgtcagaaa
    19801 cagttagtat tcaagctgtt ttctaacatg attaccttgc ttattttgct tcgcttgcca
    19861 ataattggtc tgcgtagatt ttttccaatt tttcttgaag agctgctttg attttttcag
    19921 catattcttc aaagtgttct tctttgttca tgtatggtgt cattacgctg gcacgcaata
    19981 cagttacttt tccggcacgg ttccattctt catctgagaa tcctagttgg ttcacaaatt
    20041 gtaatgggct gttgccatag tctgggatag caaagtcagt atgtgaagtt aagaactcat
    20101 taccgtaaat gcttcctttc acgtatgaag aataatcata tacgtcatgg ttcaatttat
    20161 tcattgcaac taagtcatcg ttgccttttt ctttgaatac atagtctacc atattgaaat
    20221 ctggataagt taatggatga acttcgattt ctttgtctcc tactttaaat gacaagtcat
    20281 tcaagaagtt gtagaaacga tgtgcacctt cgattgatgc acccattaat ttaccatatc
    20341 ctgtgacatt caaaggtaag acatggtgtg ctgcccaaac gctggctgct gttgcccctg
    20401 cttttgaacc ttccaagata taagccccaa gtaatgctgg gatatctgcg cctttttcaa
    20461 atacatatgt cgcaaagtaa gagataacgt ctctcatgcg gatatctttg atgacgatac
    20521 caccggcaga atatggaaca tagcccattt tatgtgggtc aatcgttaca gattctgctt
    20581 cttcgattgc tttatatgca ctgtgtactt cttctaagat ataatctttg ttttctgtaa
    20641 agacattgta tttgtaatga acatctttta aatcttcaaa tggaatgaag ttgttgtctt
    20701 cgtctaagaa aatcgcacgg ccgtatccgc cataagcagc atctacgtgt aagtagaagt
    20761 aaataccgtc tttttctaat acgcgacgca atgcaacgat tttatcgata ccgtcgattg
    20821 ctccttcttc tgttgatcct acaacaccga ctacacctag gattggtgtt ttttcagctg
    20881 ctagaccacg aacgattttt tcaagttcgt tgatgtccat gcgatagttg tggtcaactg
    20941 gcactggaat cacttggtct aaaccgatac cgatgatgtc ggcagctttt agccatgaat
    21001 agtgtttagt ttgtggaacc aaccattttc ctaatttttc taaatgtttt ccgctacgtg
    21061 ctgagtgagc tttgatttcg tcgatttttt ctgggacgct gtctaacaga ttcatgattt
    21121 cttctgttga caagttcatt aattcccaat cgctttttcc agcaactagt tcaggtgtca
    21181 cttctttcat tgcaagcggc aatgatttga tgttgcgggc ataccaaagt ccttctaagt
    21241 ttgctaaaga accatcagca acgatgtggc cccagccatc tttatagctc attaattttg
    21301 caaattccat tcctacttct tcttccattt ggcttgttgc aggagaagat tcataggcaa
    21361 cgttattacc attccatagc atagcaaaat tgtacgctag taaagatggc atcaatgttt
    21421 cagagttcat atgaccccag taacgtccgg cattatgcca aggaacggaa tgggtacgca
    21481 tccgcgctga aatttctgat agaacatcct ttgttttatt cacagtgtgt tcaaaactag
    21541 cactgctttt ttcttctggc gtaataatag gcatgtcttg aggcatgtag ttttgacgcc
    21601 agcctaaatg ttcatccacc aattcattca acaaagcttt atagatttgg ccgttctcag
    21661 ctttgtcccc aatgaatagg gcatttaggt ttagatcttt cgacaatgat tcactcatag
    21721 tgaaaccctc ctaaaaaaat atttaaattt tgtcgagccc tacattacac ctgttcttct
    21781 attctgtaaa ctaagttttg gcctgatttt taatcatatt ttaatatgta tcaattatcc
    21841 gaattgttca cacttttgaa ataattaaaa aaaacgaggg aatttacaaa tgtaaactcc
    21901 ctcgcaaaga ttgtattaac gacaaccagc gttctatttt aggatgggtt tcaaaattgg
    21961 aatgttgtaa tagtatttcc ctttttcaag gtaaaaactt actaaattct aatcaattac
    22021 tttggccaaa aagtaatttt tcttaccttt tcgaataacc acaaacttac catcgaattc
    22081 atcggatgga tttatgacaa aatttaaatc ggtaacgcgg tcaccgttaa tacttatagc
    22141 tccgtttgaa acatcttcac gtgcttgacg tttagatggt tcaatttttg tggaaacaag
    22201 cagttccacg atattttcag gtgtgcttga aatttcaacg ttcggcattt taccgaatcc
    22261 ttgcgcgatt tcttccgcat ttagttcttt aatatttcca gagaacaatg cttgcgttat
    22321 tttttgcgct tctttcaagt cttcttcact atgcacaaat cttgtaactt cttctgccaa
    22381 acggcgttgt gcttcacgtt tctcaggttc tgtctctact tttttcgcta agtcttcgat
    22441 ttcttcttga gacaagaaag taaagaattt caagtatttc actacatcgc gatcatcttg
    22501 attcaaccag aattggtaga actcaaatgg tgaagttttc ttcggatcaa gccaaacggc
    22561 accgcctgca gtttttccga atttcgttcc gtcagccttc agcatcaatg ggatcgttaa
    22621 accgaacgct tctacttctg gtccttctaa ttttcggatt aggtctaatc cagctgtgat
    22681 atttccccat tggtctgctc caccgatttg caattgcaca tcgtatgttt tatgcagatg
    22741 caaaaagtcg atagattgca gaatttggta agtaaactct gtaaatgaga ttcctacttc
    22801 caaacggctg gcaacgatat cttttgctaa catcgtgttg atgttgaagt ttttaccgta
    22861 gtctctcaaa aattcaagta aagagatttt tgacaaccag tcgtagttgt tcacaaatgt
    22921 gatatttgca tctttaccaa acaattttct catttgattg gaaagtgcgt ccacgttatg
    22981 ttgcacttgt tccatcgttt gcaacacacg ttctgttttg cggccgcttg ggtcgccgat
    23041 cgttcctgtt ccgccgccaa tcagaatata tggatgatgg cctgccaatt ggaatctttt
    23101 catcatcata aatggaatca aatgtccgat atgcatcgaa tcaccagttg gatcaacgcc
    23161 gcaataaagt gaaatgctct tttcttcaac gagctcgcga agtctttctt cgtttgtttg
    23221 ctgattaatg gcgtcgcgcc aagttaattc atcaataata ttcattttac tgacctccgt
    23281 gtagtttttt tatgaaagca ggaacctgct agcatgtgta attaattatt tcttcaggat
    23341 aggataggaa ataaaaaaag tcccttggca tataagccaa gggacgaaat ctcgcggtac
    23401 cacccaattt gtatgtctag atatccaaca tacctctcga acatttaacg ctgtcgcgtg
    23461 gcaatcactg ccaaagactc ctgaaatgta attcgcttta aagcatgtac tggtttgcag
    23521 cgaccaccag ctttctgaaa cattggcttt aacaactact tcgtttcgat cttcgtcttg
    23581 ttattattaa tatgtgtcta tcatagagcg caactttttc tttgtcaaca gaaaaaggat
    23641 atttttttta aattcctcat tttttctttt ttgaatccgc tctaattccg aacattttat
    23701 tttcaattat tgttaaaaga aataactttt tctgtatttt ttctgcaatt tatcgagaag
    23761 tcactttact ttgcctctta ttctttcttc gttacaataa ggaatgggac aagtaaaaaa
    23821 atgaaaaagc aagggacgag gtatatggaa aaaaagcaaa aaattatttt aggagccact
    23881 tctgctttaa ctatcgttat tttaattcta agtttgtttt ttgtcatccg atcatctaca
    23941 aaaaaagaac caatcattcc tgaaaaaaaa ataacagaaa aagcaaaaga accgacagat
    24001 gataagcaac aactagttac tcaagcaaaa aatctggctc aaagctacga ttatgaccaa
    24061 gcgattgcgt tattggaaaa agatagtaag aacggatcta ctgaaacaca acagcttctt
    24121 caaacactaa aaaaggagaa agaacaactg atcgactggt ctgatcctac tcagatctct
    24181 catgtctttt tccacagctt gatcgtggac ccgcaaaaag ccttccactc tcaacaggca
    24241 caaggataca aagattatat ggtgactgtt gaagaattca accgttcgat cgaacaactt
    24301 tatcaaaatg attttgtttt agttaattta aatgatttga tccaaaaaga cgaacaagga
    24361 aatctttcct ttaacgggct tgcgctacct gaaggaaaaa aaccattggt tctttcacaa
    24421 gatgatgtca gctattatga atatatggat aattccggtt ttcctagccg attggtcgta
    24481 tcaaataaca atgaaatcaa aaatgtctat aaagagaaag ataaagaaga aatcggtgac
    24541 tttgacatgg ttcctctgat cgatgccttt gtcaagaaac atcctgattt ttcttatcga
    24601 ggcgcaaaag gtaccttggc cctgacaggt tacaacggag ttttaggtta tcgaacatca
    24661 aaatctcaat atggagacaa tgaaaaaaca aaaaaagaga ttaaagaagc taaaaaagtg
    24721 gcggaacagt tgaagaaaga cggctggagc tttgcttccc atacatgggg tcacttgaat
    24781 atgactcaag cttcactaga agatgtaaaa aaagacaatg aattgtggca gcaagaagtt
    24841 gcacctatcc taggaaaaac aaatattctg atttatccat ttggtgcaga catcagcgac
    24901 tggcagcctt attcagcaga aaatcaaaaa tttgcttact tgaagagtca aggtttcgat
    24961 attttctgta atgtcgatgc ttctacacca gcttggggac aattaggtac ggatttctat
    25021 cgaaatgctc ggatcaatat cgatggcatt cgttttcaat ctgatttaga cggaaaaaat
    25081 cctattcttg atccattcat caatgtccat gatgtctatg acaaagaagc acgcgaatct
    25141 gcttgatttt acgtcattta tgaaccaaaa aagccgaact gacattttat cgtcagtttc
    25201 ggctttttta tgctttattt cttcttcggt gcgattccct tgatcaggac ttctgtccat
    25261 tctgctgtat atggaagcag ttttgcgtat atctccggct tagaaattcc ttctaccatt
    25321 gcttgaaaat agaggatcaa gaactcatcc gaatatttca aatccacttt tccttctttc
    25381 cgccctcgat ggaaaagatc aagcatgata ctgaaacttt cttgcgtata cgcatccatc
    25441 gcgtcagaaa attctttttt ttcttttttt gtatagtatg tcatcaaatc gatataaaat
    25501 tgtttgttca ctcgattgag ataacggatt ttgttttcac tcatggcaat cagtgtttct
    25561 tcaaatggaa gattttccgc aatcaacgct ttcgcttctt gccctaaatc attcataaat
    25621 gcaaaaaata cttcataaat cagcccttcc ttgctgccaa aatatttaaa aatcgtcgct
    25681 ttacttacct gagcgttctc agctacctta tccattgtca ctccagcggt tcctatgtct
    25741 gtgttcatca agtcaaaagc tgcttgtaaa atctctttct ttttctcttg tgctctcttt
    25801 tcaaaaccgt tcatttgtag agcctcccgt aattttaaac ttttataata tatatagttc
    25861 aaatttattc tgaggtttat tataactatc tattgagaaa gtatcaagct agcaattcct
    25921 tgtttatgaa ttcattgaca gaaatttata aaaagaatat tatgtactaa aataataaaa
    25981 aaagttttat aaaaaggtat aaaacctttt tattaaacta tttaatcttg tttagtatat
    26041 atttttagaa aaaggagtgg ggtttttatg gcagaaatcg tcaaagtcac acatttgaaa
    26101 aagagctttg gtcgtttcga agctttaaaa gatgtctctt tcaccgtaca ttcaggtgaa
    26161 gtcgttggat ttatcggacc aaatggttct ggaaaatcca cgacgattcg gacactttta
    26221 gggattataa aaaaagacgg cggaacggca acgatttttg ggcaagatgt atgggaagat
    26281 agtttatcta tccatgaacg tctttcttat gttccaggcg atatctcttt gtggggaaat
    26341 ttgactggag gagcgatcat cgatctcttc atgaagcttc atggaaaagg gagtgctcaa
    26401 aaaaaagatg aactgattca acgtttctct cttgatccta agaaaaaagc aaaaaactat
    26461 tcaaaaggaa atcgccaaaa agtgggcttg attgctgctt tggctgttga ttcggatctc
    26521 tatttatttg atgagcctac ttctggttta gatcctttga tggaaacgat tttccaagaa
    26581 gaagttgaaa aattgaaaga acagggcaaa tccatcctac tttcttctca tattctcagt
    26641 gaagtcgagc gactagcgga taaagtcgtg atcatacgcg aaggaacagt cgtagaagaa
    26701 ggaacactcg atgaattgcg tcatttgact cgttcgacca tcactataga aacagaagca
    26761 gagaatagtc acttagcaca attgaacggt gtccatgact ttgtgcaaaa aggtcacctc
    26821 gctcaatttg ctgctgataa cgaagctttc gatgtgattt tagctgaagc gtccaaatta
    26881 ggtgtgaaaa aatttgaatc tattccaccg actcttgagg atttattcat gagacattat
    26941 gaaggatgag gaggaagcga aatgactgat aaattttcac gctggtccac ccttttttct
    27001 caaaacctta gccgtgactg gaaaaagatc ctgatatggc tagtcggtgt cactgctttt
    27061 tctggtggtt ttgtccctgc gtttgaagaa ttgacttccg gacaaggatt agctggatta
    27121 tacgaaacct tgcaaaaccc ggcaatgatt gcaatggtcg gaccaactcc agcttcgaat
    27181 gctgctgagt atactttagg agcgatgtat gcaaatgaaa tgttgctttt ttgcagtttg
    27241 tttgctgctg tgatctctgt tttacacgtg gtgagccaaa cgagaaaaaa agaagaactt
    27301 ggtctgattg aacttattcg ttcctttcga gtcggacgtc aagcaaactc tttagctgtc
    27361 atcgctgaaa gtattctgat caatttgctt ttagcaatat cgatcagctg tacgatgatt
    27421 tttttccaag cagattcgat cactgcagaa ggtgcttggc tttttgggct atctgtaggt
    27481 agtatgggga ttttggggag tgccgttgca ctgtttgctg cacaactgat gccaagttca
    27541 gccagttcga atggtctgtc tttagcgttt ttaggtggac tttaccttct acgagcaata
    27601 acagatgtag ctgattcctc cctttcgatg tggaatcctt taggctggtc ttatttgacc
    27661 tatccattta caactaacaa ctggattcct ttgctttatt tgtttttgtt cagtctaatc
    27721 ttgataatca gttcatttgt tttagaggaa catcgagatt tgaacaccgg ctatatcccc
    27781 gaacgtgaag gaaaaccgta tgctaaacgt tctcttttat ccttgcgtgg tttattattc
    27841 tatctaaata aagggatgac aatcagttgg cttatcactt ttggtttgat gggggcggca
    27901 tacggctcta tctacgggaa tatgcaacag tttttagaaa gtaacgactt aatgaaacag
    27961 atgtttatcc aatctggcgt ctcaattgaa aaaaacttta caagcacgat catggtcgtc
    28021 ttgatttgtc tagctgctat tttaccaatt gctagcatca atcgtttatt tacagaagaa
    28081 acccacggac gtatgaatca gctgttcgca acaaaaattt ctaggaacca actttattgg
    28141 accaatatgt tactagctat tctatttgct atattggcaa tcctgattag ttctggtgca
    28201 ctagggcttt ctgctttggc ttcgatgaag gatgcgaaag acatggatct attgatgttt
    28261 cttaaagccg ggttcaattt cctgcctgcc gttttagtgt ttatcggaat tagtggcttt
    28321 ctattcggat ggcttcctcg aatgggtaaa cttgcttata tctacttagc ttactccttt
    28381 gcactcaatt actttggcgg aattttggat ttaccagaat ggttttccaa aacagctgtc
    28441 cctagctggg ttccacggat gccaaacgat tcttttgatc tttccgtgtt ccttacgtta
    28501 tcatgtatca gccttgcact gttcgtcctt ggttctattg gttataggcg aagagactta
    28561 ctagaaggat aataaaaaag catgtacaat ttcatgctag cctccaaatc tgggtcaaaa
    28621 atccaacatt tggaggtcat ttttttattt acaagtcttc taactgctct ttatcaatga
    28681 tccagacttc tcgattcttt ctttttacat aaccattctc ctctaataat ttaaattttc
    28741 tagataaagt ttcgggggtc gtaccgataa atgcagcaag ttctttcatt ttcattggca
    28801 ggaaaaagtg atccttccct gtcgctttag ctaagtcaag cagatagaca gcgactcgtt
    28861 cttctacttt ttccatcatc aaaaaagttg tctgctgctg tacttttgtt aatttctcaa
    28921 cgttgattgc taaaagtttc agactaaggg cgggataatg cagtaagagt tccgagaaat
    28981 ctttttgtct caatatgcaa atttccgttt tttctagtgc ttcgccaaat aaagactcat
    29041 ttggaacgcc tagcaactgg ctctctccct catatccgcc cggctctatt actcgtaaaa
    29101 gctgttcctt tccgctagca gataattgat acactttcat actgccggag gccacgatca
    29161 ctaattgagg ttctccattc ggcgcaagaa tctgttctcc tttttccaca tagtgatgtt
    29221 tgacaagtgc gtgtactttt tcttgatctt gctgtactaa gtgattgaac aaaggaacaa
    29281 atgaaacaca taaatgatca tgactcataa tctctcctcc ttctaaacag ctatttctaa
    29341 taaagaaaca taacttccct tattccaact gataattttc ttttatcata gcataaacgt
    29401 caagcgaacc agataccagc aaatagagtg gatttctttt tgtcccttgc tttattttcc
    29461 ttcttttgag accttaagta attgattcct atcaattttt taaatattta ctttttgaat
    29521 aatcaaaaac aaaccttcac tcgcaagccg agagtaaggt ttgtttttcg cattgcttat
    29581 ttcgtgttgt ctattgaaat ttttccgcac ctagcttttg atatagtggt gctagttctt
    29641 gtaaggtttc agcggttatt tgctcaacgt gttcgatttc ttgcggatag aagaatagtt
    29701 tacccatttg aaaggttcgg tctctcatcg cttttttcaa ctcttcttgt ttctttgtat
    29761 cggacaaata gtcagacaaa gctaagtggt cgtcaagttc atgttctggt tgcggccata
    29821 tgtaataatc cttgatatcg actgttttgc tccactcttt cagtatcgca agcaactggt
    29881 tatacgctgc aatgctacca tatcgttctt cgcttttata atgttggtac atcaaataga
    29941 tttggtattg tttttgattc aacaaaactg ctagacaggc attctcgtct tgacgagatt
    30001 cgcttcggta cgcgctccaa aaatgactgc gtaaattcca gccattcgtc cagctttcga
    30061 ttttaggttt gctcatgccg tacatatcag gcaaaagtgc agctgtctgg agctgaatct
    30121 ctttccattt ttgccagtgt tctttatatt ctttcttgat ttgttcaatc ttatctgttg
    30181 gatggctctc tttgagctgt ttgaatgcgt agattggtcg atcaaacaac tgataatctg
    30241 attttccaag ttccattgtt tcgcctcttt ccgttatctt ttgtattcta cctgaatttt
    30301 gcgatatgga caaatagagg ttgtgaaaaa gctgttctgc atcaagtcat aagaacagcc
    30361 aaacaaaaaa ctgcgatgag catcaccgca gttttattga acttaaggtt gattgatcac
    30421 atctaaaagc gttgaaaata aaatggcact agaagcagct cctgggtcga tatgtccgat
    30481 tgcacgctct cctagatagg aagctcgtcc ttttttagct accaagactt tggtgtgttc
    30541 ttttgcctgg tcaatgattt cttttgttaa attccctgcc ttcaatgcct ctgtgactgg
    30601 cagccaaaca tctaacatcg ttttatcgtc agcttctgcc tttcctcggc tttgaatacc
    30661 cgctgttccc tgctcgatca cttcgcctaa tttttcttga gaatcaatag aatcgacctc
    30721 ttttgtcgct ttcatcatgt tcatgaatgc agatccgtat aacggaccag aagccccacc
    30781 gactttcgat atcatcgcca tagagaatac tttgaatgta tcgctgattg tttcaggatg
    30841 atcggcttga aaagcttctt cataagctga aacccctctt cccatgttat ttccatggtc
    30901 tccatcgcca atcggggtat ctagatcact gagatattgt ttgttctgat tgattgcctc
    30961 tgcaaattgc gtgagccatt cttggatttc ttgaacggtt agtttcatat tatcaccctt
    31021 ttatcttgag attttttacc atgaaatcgt ctgtacattg ctttctaaag aatctttcca
    31081 ttgtgtctcc gtcaagtcga tcagtgttag agaaagacct tgcatatcta gtgaggtcat
    31141 aaaattaccg acttttcgga aaactacatt gacgcctctt tcttccaaca gagctaaaac
    31201 atcattcatg aacacgaact gttccatcaa aggcgtcccg cccattccgt tgactagtac
    31261 acctgcttct tttggtaaat tcgagtaatc atctaaaatc ttagtaacaa gttcctttgc
    31321 cagtacttta gaaggctgca tcttttctcg acgatagcct ggctcaccgt ggataccaac
    31381 accatactcg atctcatctt ctcctagttc gaatcctggt ttccctactt ctggtacagt
    31441 tgctgctcga agcgctagac cgatcgtctt agttgcctgt acgacctttt ctcccagttc
    31501 tttcaactca gctagggaag caccgtttct tgctgcatcg cccacgattt tgtgtacaag
    31561 gacagtacct gcaactccgc gttttccagc agtatacgta ctattttcaa cggcgatatc
    31621 atcatctacg atcacacttt ctacttcaat atcgtccata gcagctaatt cctttgccat
    31681 atcgaagttc aatgcatccc ctgtataatt cttaacgatc agcaaaactc cttttccact
    31741 atccgcttcc ttaatagccg tctgaatctg atctggagtt ggagaagtaa acacatcacc
    31801 aagcaccgct gcacttaaca tcccttcccc tacaaacccg gcatgggatg gttcatgtcc
    31861 gcttcctcca ccagaaacca aaccaacttg ttccgctttc tgattcttgg ctacaacacg
    31921 ggaagattct acacgatgaa caagttctgg atagcttttt actaatcctt ccagcatttc
    31981 ttcgacaata ttgcttggat cattcatgat ttttttcaat ttattcccat cctttctttc
    32041 cggcattttg tgcttctgtt aagatttgct gagtatcatc tgttataccg gcagtgatgg
    32101 ctgctgcaaa agctccttcc actaaaggcg catccaccag atgataacgt tgctgctgct
    32161 cttcttccaa catgtctttt gccagttccg agttcaatac cgcgctaccg atatccgcaa
    32221 aaacaaaata actagctgca tcgcttgatt cattgactgc ttctacgatt ttcatcgggt
    32281 ctgatccgat ctcgccattt attccgccga gagaaaaaat ttgcacctct tcggattttt
    32341 gcatctgctc aatcatttct ttaataccat ctgttacttt ttgactatga gaaataagca
    32401 aaatactttt tttcacaaga atcttccttt cttttacata gttgctaacg attggtttcg
    32461 ctttcattaa tgaacaattc aagtatataa taaatttcta attttgggaa gatatgtgta
    32521 gtgcttcttg aagtttgtta tactttaatt aaaagagagg agtgatcgaa tgattacaac
    32581 aacaacgaac agtgtagagg gaaaagccgt agtagcttat aaagggatcg tttttggtga
    32641 ggtcattact gggatccatg catttaaaga cttggaggcg gggttgcgaa atatctttgg
    32701 cggccgctca aaaagctatg aaaacgaact aatgggtgca agagaagaag cacttgccga
    32761 aatggccgaa cgtgcaaaag cattaggagc tgatgcgatc attggcgtaa aaatggacta
    32821 tgaagtatta ggcagcgaca atggcatgct gatggtcaca tgtagtggaa cagctgtctc
    32881 cacgagataa gtaggggaca tctgttttaa taactaaaga gagatgggac atgtagtggc
    32941 actcaagaat tagatttttc agtctaactt cgaggtactc taccttccca tcctctcttt
    33001 tttatttctt tttgcggatg attccgtaag ctgctgcgac taacacaccc gcccctgcta
    33061 aggaataact gacagaagct gttttttcat ttgtttttgg caaagtctga cttgttgatt
    33121 tggatggctc tgtcgtttgc tgaactgttt ctgtcgtgct tccagaggtc actgattttt
    33181 ctcctgccga atgcaaaata tcttttattc gttggaaact agctgctaag tcagcttttt
    33241 ttgcttcaaa atcattagca actgtttgtg cttcgctgat ctttccgtgg attgcttgac
    33301 tggccgctgt gatatccgct tgagtttgac ttagctttcc agaagctgtt tgagcaacct
    33361 gatttaattg ctgattgaat tcttttgctt gttggatctt cccgtcaaga tctgtttgga
    33421 cagcttccgc ttttgctttc gcttcggcaa gctttttctc aagttgtgca cggtcatttc
    33481 ccactttatt tttaagatca gctaaatctt gatcgattgc tgcttggtct tctttcaatt
    33541 tgttttttgc agcttctaat gcttcttcga gttcttttct tttattttcc aagtcttgca
    33601 aatgatcgat ttggtcagaa gaggtagaac tatttattga tgtatcttct gaagatgaaa
    33661 tcgtttctcc tgaattatct gtcggtacat tggaagtcat ttctgtttcc gaggcaccgt
    33721 tttcagcacc agaagagctt atagttgttg aaggttctgt ttgagcaggt tcagaagttt
    33781 ccgcaaaagc tgtctgtccc aataagaaaa tactggaaac agcaattggc cagattaacg
    33841 gcttcattct ttttttcatc acttttccca tccttactac tttttaacta atttcttatt
    33901 ataagcaaaa atgtttggaa gaaccataaa cacaacaggc tgtttgtcta aaaaacggtg
    33961 catcttttat ttttttgtac gttccgggct tttccatgtc ccgcttccat aacctaaaat
    34021 cgttttgact gctggaatga aagtcgtcac gatcgttatc gcgttcacta tccagaacaa
    34081 aagcatatat agtggtgcga aaagaaggta tttcaatttt cttcgtcgat catctacaat
    34141 cagcgaagct agcaactgga aaaatcctgc gaccatttca aagcagatga acacaaaact
    34201 gaatgtaaaa gtaatataaa tctgttcgta gtttccttga tatacataat gcccgattaa
    34261 tccagcaaaa agaactacgg aaatacaaaa gaatattgac cagacaatac tcaatgtttg
    34321 atcaatgaag atgatcgttc ttccaatatg tttaaaagga tggagcatca cttttttgaa
    34381 attcgtcagc cagacttctg ttccaccttt tgcccaacgt tttctttgtc gatatagcat
    34441 cttcaacgtc acaggtactt ccatgaaaaa gataatctca ggagcaaaaa cagataacca
    34501 gtcatttaac tgatgatccc aagcaatact gatgtcttct gtcgctcgat cttgtctgaa
    34561 tccgcccaca tcgatcaagg cttcttttcg atacatcgta tttgctccac tgtacgcata
    34621 aagaccaccg aaaacagctg actgtgttcg tttaatgatg cccacgatac ttgagaattc
    34681 aaccgtttga gattttgcaa tcaatttggt acgattttgg acatccatat ttgcagtgac
    34741 cgcagcaata tgtcttgcac cagggcgaat gaaataattc acatacctaa tcagtgcatc
    34801 tggctccggt accgtgtctg catcattgct taaaatcaat ttcccttttg caaaggcaag
    34861 tccgatatta aacgcgtggg cttttccttt atttttttcg atccgaacca cacgaagatt
    34921 agcgtatttt ttcatcagtc tagctaatat ttctggtgtt tggtccgttg aaccatcgtc
    34981 tgtcactaat acctcatagt tgtgataatt caatttcgtc atcaaatact cgattgtatc
    35041 ttcgatcaca atttcttcat tatgggctgg caccatgatc gtgatgaacg gttcgacact
    35101 caagggaaca tctacccatt ctttttgctt atgtttgtat aaaaacacat aacaccagac
    35161 accaataaac catacaaatc cgcctaaaat cggataagaa acgagaaaaa atacataata
    35221 gacaaacaag tatctaagaa taaattacct gtatgggtga ttgagatcat ttcagttcca
    35281 ccccattttc tttatacaaa tctgcaatga atgtcgtatc tagatttttc tcttcggaga
    35341 cagaataaaa acggaccgtt tctctctctt cttttgttcc aaaacgttcc gtatacactt
    35401 cttcaagtaa ttcttgtctt tttttcaatt tttcatcttc atattcaaaa gatttattta
    35461 cttttttgta tagatttcga ttgttccacc tagtcaatac aaatgaggta atcaggaaac
    35521 tgaaaaaaat caatacaaaa aagccgatta agaaatacag cattttcttt tctgataaaa
    35581 aagcgtacaa ggaaaaatcc ttggctcttt ctggtctcgt taatgggaaa aagacccata
    35641 taaaaggaag aacaacgatc atccatccta aaagagccac taacgtttgc caaatcttta
    35701 accagtaatt accttttgaa aaatacttat ctttcaccaa aattgatgca acttcttctc
    35761 ttgttacttt tttcatatcc tcacctcacg tttttttgtt acgacgtagg agtttaccat
    35821 acatttcatt tcttttcaca taaagtgttc ctaaattttt cctaagaaaa agaacaaaac
    35881 atttctcatc agcaaatgaa aaatattttg ttctttttat cagttgttct ttttattttt
    35941 tatctcttaa ttatgttgct ctttccaata cgacaatgct tttcattcct ctagatatcg
    36001 ccgtgtacat ttgtctgcgt cttctttgct catctccatc gttttcaaat ggatataaga
    36061 ttacatgatc aaattccagt ccttttgcta gactcaaagg aatgatttgg cagttttcta
    36121 atccatatga atttttttta atcgattctt gtagcgtttg ggtttcctct tctgtttggg
    36181 tgataactgc cgctttttct ccttttagag aaaccaaaat ctcagctagt attttcaaca
    36241 attcttcaga ggatcgaaaa gagcggtatt cgggttcttt tccttgcggt cggatcgata
    36301 cgatgtcgat cttctcttga tccacagcat aggttttgaa aagctctgtg attgctccgc
    36361 tagaacggta actatttcta agatcatacc gatggatcgg cagatggtag tcgtcgaaac
    36421 aacgcataat atcagcaaaa gtagtggaag aattaaagat tgcctgattc tcgtcaccta
    36481 ctaaagtaaa gtgagttttt ggaaataatt cgatcaatag acctaattgt gccagggtat
    36541 aatcttgtac ttcatcaatc aatagatagc gaaatgctgg tacatttacc ttttcaacaa
    36601 gtaaatgacg aatagctaat ataatgactg cttcatcaac agtacgtgtg ggttgccaag
    36661 cataagctga gccatatcgt ttttcataaa ttctttcaaa caactgatgc tcatccaccc
    36721 atgccatttc ttcgatttgt ctggttattt ttcgatattt tttttgtaaa agtttccttg
    36781 cataagctac aatactttgt tccgaatcat cagaaatcaa tttaccgaat aattccaact
    36841 gcctatcttc tgtcaacgag agtacctgat tctgcacagc aggacttttt gcttgtttca
    36901 aaagatgatt cttccattcg ctcatcagtg cctttttcgt tgcttgaagc ctatcgatca
    36961 atttaggatg agcaggaact ttctcatata ttttttcgat cgttttttta gaaaggactg
    37021 tttttccatt tttagtaagt ccacggataa aattcggatg atctaaaaac aaaggatctg
    37081 attgttttag attatcgata aactttttgc tacgcaaacg ttctgtctgc tccgatacat
    37141 tttctcccgt gatacgtttg aaataagcct cttcaccttc tatttcctct gctgaaagct
    37201 gtgaaaccag ctgtatgatg gttagattca gtggatttct ttcacctaat gatggtagga
    37261 cattcgaaat atagtcgata aaccgatgat taggtgatag aatcagcaag tcatcggatg
    37321 tcatcgtatc tcgatataaa tagagcagat acgcaatacg ctgcatgata gcagaggttt
    37381 taccacttcc tgctacgcca tttaccaaga tattttgact tttcacatcc cgaatgattg
    37441 tgttttgttc cgattggatc gtagcagtga tagccttcat ttcattggtc tcatcttggc
    37501 ctaagacttc taataatacg tcatcttgaa tagctacaga cgtatcaaaa aacttgacca
    37561 aacgatctct ttccaaaaga aattgtcgtc tttctttaat aaccgtatcg attttatttt
    37621 gacgaaccgt ataagaagat ggaccaatca tgttattgta aaaaagttcg gcaattggtg
    37681 aacgccaatc ataaacaaga ttttcatctg catcatctgt gaaatttgct gtaccgatat
    37741 aaaaggattc ttttggctca ttatccagaa agtctacaac gacttttcca aagtagggac
    37801 ttttcaataa acgttcgatc tttgctagct gattttctgc gtttttctgt ttgatattca
    37861 tctgatcgat ttctctattt ttcatttcaa tcatcgaaaa agtatccagg ttatctaagt
    37921 aactgtcaaa attcaatcga agctcgccag ccatttcttt caacgactgg tttccttcta
    37981 tttttgccgt atccatcgat tcccgcagtt cgtttttttt atcaattagc ttttgataca
    38041 cgttcttcag atatgcttct tctaactgtt tttgtgtgtt gaccatacac attctcctct
    38101 ttttttaaag tggtggaacc gattagttta cctcttttaa gagtcgaatg caagcttttt
    38161 aacgagtatc atttgttagg atatatcagt tcttcaggtc ggacagtttg atataaagaa
    38221 aatgcaccat caaggttttt tacgtggtag ccagactgct tcaagattct ttccgctaga
    38281 tagctacgta atccgctatg acagctgacg atatattctt gttggctgtc taattcattt
    38341 aatcggtccc gaagttcatt caaagggata tgtttggctt ccttgaattt gcccttttcc
    38401 aattcttctg ctgttcgcac atctaataaa atttttcctt ttgctagttc tgtcggcaat
    38461 tcatgccatt gtacggtttc actaagccct tctgcaatgt tcattgctgc atatcctagc
    38521 atgtttactg gatctttggc tgagccaaat ggcggtgcat acgtcaattc taattccggt
    38581 aaatcgaaga tcgtcaaatg ccctttgatt gctgttgcta aaatatcgat ccgtttatcc
    38641 acaccttttg ccccaacacc ttgtgcacca taaatctcgc cagtctctgg atggaagatc
    38701 aacttcagta aaatatctgt tgcttctgga taatagcttg catgatcttt tcctgataca
    38761 tgaatgactt caaaagacag ttcgttttct ttggctgttc tttcacttaa accggtggat
    38821 gcggcagtca ttccaaacgt tcgtataatt gctgttccaa taccgccttg atttcttcga
    38881 gaaattccgg aaatcgtgtc cgcaacttgt ctaccttgac gattggctgg tgaagcaaga
    38941 gaaataagtg catcttttcc agtgatttgc tgttttacaa cgatagcatc ccccaccgca
    39001 taaatatcag gaatattcgt ttgataacgt tcgtctacta ggataccccc gcgtatgcct
    39061 aattcgattc cagcggtcac tgctagctgg ttatcaggac gaacaccaat ggataaaata
    39121 actaaatcag cagggatggt acgcccatca tccagtcgca atcgtttccc gtggtcttca
    39181 aatccagcga cagcatgaga agtaatcact tctactcctt gatgagacaa ttctttttct
    39241 ataaaagctg ccatttcttc atccagtgtt ggaagaatat gtggtgcttg ttcgatcacc
    39301 atgacttcta aacctcttct ttttaggttt tctgccattt ccaatccgat gaatcctgca
    39361 ccaacgatca cggcacgctt tggctgtttt tctaatgcat gcatcacttt atcgatatct
    39421 ggtacatttc taatagaaaa aacattgtcg gcttctgtta tccctggaat cgatggaaca
    39481 actggttttg caccaggaga taaaaccaat gcatcgtact gttcaatgtg tttttgaccc
    39541 tcatgtacga cttctaccgt tttgttttcg ggaaagatgg ctgtcacttc atgatttgga
    39601 cgcacatcta aacaaaaacg agcggataat gattctggcg tttggacaag aagattttcc
    39661 cgttcactga tttctcccga aagataataa ggcaagccgc aatttgcaaa agaaacatac
    39721 ggtccttttt caaaaacaac gatttctgca tcttccatta atcggcgaag ccgtgtagca
    39781 gcagacattc cacctgctac acctccgaca ataacgattt tcatcttatt tccctcctac
    39841 tgttcgatca aaccattgat tcatgccacc acgtacattc actacatcat aaccagattt
    39901 ctttaattct ttagctgctt gtttactcct catccctgat tgacaaatca catagacggt
    39961 cttttctttg tctccatcat atctgtttat actttgtaat ggaacatttt tagctccttt
    40021 tatatgcccg ccccggtatt ctgcaggtgt tctaacatca agtaacgctg gattttccaa
    40081 aagcttttcc tttaattctg ggactgttat cgattttatt gattgaaata gcaaattcat
    40141 tttctgcacc tcatttacat ttttataaat acctcatggg gtatattaaa caggagcttt
    40201 attaatatgt caaccagaat gactcttcat ttttttcact actttttata tttgcataaa
    40261 ataccgataa ggggtattat ctaattataa aaatagagga gaatgtgtta tggagattga
    40321 taataaaaat atcctgaacc gtttgaaacg tacagaggga caaattcgtg gtatccaaaa
    40381 aatgatcgaa gacgaaaaag aatgtatgga tgtcatcact caattaagcg ctgttcgttc
    40441 tagtattgat cgcgtaatgg gcatgatcgt tgcagataat ctgaaaaatt gctttgagaa
    40501 acctgatagt aatccagaag aacaagcggc taaactagaa caagcgatca agatgattat
    40561 taaaaagtaa aggaatgatt tcaatgaaag tcgttttcca tattgatgaa ttagaaaaat
    40621 ggtcagaaac agggaaaaac gtaaaaaacc tgataaaagc ttctccagaa acgacgattg
    40681 tcgtatctgt aaatggcatc gcgattaccg gttatctaga ctctgccaat gcagaattct
    40741 tagatttgca aggagtcgtt tttcacgctt gcgccaatgc tatgcgtgct aatcatattt
    40801 cagaaagttc tttacctgaa caagtaatcg ttgtaccagc aggtgtcctt gatttagttg
    40861 aactgcaatc ccaaggttat gcttatatca agccttagtc agtagaggtt gcgacagaag
    40921 tgatcagctt caagaaattt cggcttattt ttcgaagaag ctacttctgg aaaaccgtga
    40981 atcaaaggtt gtgaaaacgc tgtcctgcat caagtaataa gaacagccaa acaaaaatag
    41041 cttctcatat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt tgaacaccgt
    41101 ttaaggaaga ggttgtgaaa tcagcggttt gacctaagta ttagagaaaa aaacgggaaa
    41161 atagctcctc atattttgcc gttttttgag ctaataccga aggtcagctg attgaacacc
    41221 gtttaaggaa gagattgtga aaaagtcgct tagtttcgag tattaagaaa aattttgtga
    41281 aaatggattt ctacattttt gcataaattt ggcttaatac cgagaaactg gcttttgaat
    41341 accgtttaag gaagaggttg taaaatcagc ggtttgacct gagtattaga gaaacaaacg
    41401 gaaaaatagc tgctcatatt ttgccgtttt ttgagctaat accgaaggtc agctgattga
    41461 acaccgttta ccaagaggtt gtgagcctac catttaactc cgaatcacaa gaaataattt
    41521 caaaaacaac ttctcctgtt tttggtttgg ttggcttatt atcgagaagt agctactggg
    41581 agtctattta tcaaaaaaag gaatgcgaca taggtcacac ccctttttta ttttctttga
    41641 aacatctttc ggtactcgac agcatacaag acaaaagatt gtaaaagacc gtaagtgatg
    41701 acggcaaaaa tgactctcac ccacagcgtt ggtaattctg ggaaaagcag caaaaggatc
    41761 atgccaacat agatgacagc agtcgctact ttgccatacc acttcgctcc acttaatttg
    41821 atatttttag atagcccgat taatccagct acaaacataa agccttcttt tatcaagaat
    41881 agaccaaaca acagccaaag ccacggtcga taccatgcca tagacaaaat cagaaaaagt
    41941 tgtgtcaatt tatctgcaaa cggatcaagc accttcccta agtctgttac ctgattatat
    42001 ctccgagcaa tgaccccgtc tagataatca gacaaacctg agacaacaat gaccgctcct
    42061 gctatataaa atgactgtct cagcacaaaa taaagataaa tcggcagcaa aaaaatacgg
    42121 aatatcgata aaagattcgg aatcgtttgt atttctttac gccagtctgt gcttaatttc
    42181 attttatcgc cctacctcct cagcactctt ttctttttat tttagcttac acggggaata
    42241 aaaccaatga tttcactgtt tttatttttc tttacagcca ctgatattat gagctaattt
    42301 gagataatca cttgctgttt tcatcgttta ctcttctttc ttcctgtcgt ttgcgcattt
    42361 cttttactag tactttatga gctttttttg atcctcgctc taggtcacgc tgccgtttac
    42421 gtgttttata atcgataaac agactgtcta aatcgtaacc tgtcgggtac agatcttctg
    42481 ctgaacgcac cagacgaaca cgctgacgag gcacgtggat tatttttttg tctaaatata
    42541 catcaatcgt atcttcacct atatcttcat aaactaacgc ttctttttgg tattcattga
    42601 caaatatccg atcacctttt gcaaagagct ggcgttcttt tttattctcc ttcctatttg
    42661 taactgctgt cttaggaaga aattcttttt tctgtgtccg ataggctttt tccgccaagt
    42721 aacgctctgc ttgatggatc aatttcatcg acatatccat tttatgagca atccacaaag
    42781 cttggctttc tcctgtttct ccaacccgca actgatattt aggacgaagt gcttcccgat
    42841 cgaaagccat agcagccggc acaaaatctt catgatctcc ggcgaatttc ttgatttctc
    42901 cataatgggt cgtggcaatg atcaatgccc cttgctcata catagattcc atgattgcga
    42961 tcgctagtgc agccccttca tttggctctg ttccactgcc aatctcatct aaaagtacca
    43021 acgtattcct ttttatcttt ttcaaaatag cagcgatgtt ttgcatatga ccagaaaatg
    43081 tactcaacgc attttctaaa ttttggtggt caccgacatc gacgaaaatc tcatcaaaga
    43141 cagcaagctc tgtcccttct tttgccggaa cttgcatacc gaacatcgcc atcaaagtca
    43201 ataaaccgac tgtcttcaac acgactgttt tgcctccggc atttgctccg gtgataatca
    43261 agcctcgata gtctttaccg atttcaacat ccaatggtac cgcatgctca ggtaaaaatg
    43321 gatgtctgcc ttgcttgata atgatatgtt ccgatttatt gattctaggt gtgcttccat
    43381 tgatttctcg gctaaatttt gcccgagcaa agatgatatc taacaccgtg atcgtttcga
    43441 tcaataaatc gatcacttct tcgttttctg cgatagcacc agtcaatgcc gccaggactt
    43501 gatattcttc actgatttct tcagctttta acaactgata gtgttcattc aatttagaaa
    43561 tcacagttgg ttcaataaaa accgtcgttc ttttatttga ttcatcgata atcgttccat
    43621 ctattttatt tttatagctg gctttgatag gaatcgtata gtattcgcct ttttgaacaa
    43681 tcattgcttc ttggatcatt tctttgtttt tcggatgacg caagaatttc agtaattttg
    43741 actggatttc tttttcgatt agttgtaatt gtttgcgtac tttacgcaaa ttacgtgacg
    43801 cgtcgtcact gactttatta ttttttattt tctggtcaat caactcttct acatttatca
    43861 aatcaggaag atgtttgcta taagcaaaca acaagggtgt ctggtattga ttcttatcaa
    43921 aaaactttgt gatcatccga ctgcttctaa gaaaatccgc atattcgatc aaatcggttg
    43981 gctgcagaat caaccctttt ttgacttgtt ctgtcagtgc atcgatcctc ggcaacccca
    44041 taaatggaac atgctgactg ctttctaaga tcaagcgtgc ttctcttgtt tcttcttgac
    44101 gatccaagac agtttgtagg tttgtcgaga ccgtcatatc gctgattcgt tttttgctat
    44161 agtttccaat tgctcttgct tggatttcct ttttgatccg atcaaattgt aattgactaa
    44221 tcatttcgtt gtccatattc tctccttttc ttgaagagat acctgtccca aaataaataa
    44281 gggcctagag ataaataaaa ggatagaaca cagcttaaaa aagtcatgtc ctatccttat
    44341 tagaatacaa agatattgtt ttcctgacag gtaccgtttc ataagcacac caaataaact
    44401 ttgtttattg gctattcgac ttactgatta gataccagct acactcacaa aacatacccc
    44461 attcttctag gtcttattta ttttccactt aactatagca agacacattt tggttgtcaa
    44521 actttagtat gttttataga tttacaccat taatgaatag acacctaccg cgataattgc
    44581 tccgatcatt ggacctgcga ttggtaccca tgaatatgcc caatctgaag ttcctttatt
    44641 agctattggc aataccgcat gtgctaatcg aggaccaaag tcccgagcag ggttgattgc
    44701 atatcctgtt ggccctccta atgaaagacc gatggctaag atcaatccac ctacagcgaa
    44761 tacgttcgtt ccaggtgcaa attcgttttg tccaaaagct agcaacccaa gaactaatac
    44821 aaatgtacca attaattctg tgatcacatt tgctggataa ttccgtatag caggacctgt
    44881 tgcaaacgtt cctaaaatcg cgctttcatc tttcgttgca ttccaatgtg gcaaataaga
    44941 aagccaaaca agaatggccc ctgcaaaagc tcctaaaact tgcgcaatga tgaaaggaac
    45001 gacaagattc caactaattg cccctgtcat cgccatagct agagagactg caggattcaa
    45061 atgagctggg cccatgaatc cagaaatata gacagccatt gttacagcga gtccccaacc
    45121 aaatgcgatg acaatccagc cagaagcctg tgctttactt ttgtttaaat tcacagccgc
    45181 acacacacca tctccaagca aaactaggat cagtgtccca ataaactctc ccatgatttg
    45241 agtcatatca ctgttcattt atttctcccc ctttagttcc cgcagatctg attctgcaat
    45301 caatgcttct aaacgtttag tctgttgcgc cttctcctct tctgtccatt caaaatatcc
    45361 agcaattgca tcgatcacag gttgcttgat ctcatcaagc tgatctcgtt caaacaacag
    45421 atgatttgta cgacgaatga gataatcgcc tggggccaaa accatttctt cttctaaacc
    45481 ataacgtaac cgagctgact cagcgagact caaaccagga taaggtgcca tttcttttgc
    45541 taattcaaag atacgtcggg cattagtccc gtaaaaatca gcgatataag tagcatcttc
    45601 ttcctctaaa ccggctgcta ctccttcttt tgctaattct gtcactgttt cttctaattt
    45661 agttggatcg aagtttccac ctgaaatctg atatttttta gagtcgattt ctttcgtctc
    45721 tattccgtat tcttctttta ataattggcg tatcaatcgc aaagctcctt ctgccatctt
    45781 acggtaatcg gtgattttcc caccagataa agtcagcaaa ccatctggtt ctctttctaa
    45841 agaactacct ctggaaatcg tagaaggtgc tttttcatca cggctgtttt ccaaatggtt
    45901 caacacgtct tctacttcag cacgagaaac tttgttttcc ttatattcac ttactgtatc
    45961 gacaacttta ttgaagctct tgtctgaaat cgatccatta tctccaccat tgtaatcaga
    46021 accagaatta ccaatcaaca gtggacgaag tcctgcccag cttgcttcga tatcattcaa
    46081 tgtgatattt gcttctggat agcgatgatt gatcacatcc aatagataat ctacatcttc
    46141 ttgtgtgact ttcggatccg taaaatctcc ttggtaatcc gtatctgtcg taccaaagta
    46201 agtcttgttt tctcttggaa tagcaaaaac catccgccca tcttgctttc ctgtatcgaa
    46261 gtatgtgggt tgcggtacag gcagtttttt cgcatctaca actaaatgga tccctttagt
    46321 tggacgcatt ttaggagaga ctggacgcgt aaaattcaag tttcgtactt tatctaccca
    46381 aggaccactc gtattgatca ctaattttgc tttgatttcg atcacttcat ctgtcagcag
    46441 gtcacgagct ttgacgccaa cgatttgatc gccatcatat aaaaagccaa ccgctttcat
    46501 tttacttact agataagccc catcttcggc agcctttttg atattatcaa tcactaaacg
    46561 ggcatcattg ttgcggaaat ctagatagac acccgcgcct tttagccctt cttttttcaa
    46621 aaacggttct ctttccaata cttcttctgg tgtcagcgta taattctcat atttggttcc
    46681 tgtcacattt gccagtttgt cataaaggtc cattgctact ttgaccgaga acatattgaa
    46741 ggttgttgct ccttcgccct catagattgg caaaagcatt ggatctggtt ttgggatgtg
    46801 tggggcaata ctttgtacga ctgcacgttc accaactgtg tcagctacta cttccacatc
    46861 aaatgtcttc agataacgaa taccgccatg aacaagtttg gtcgagcgag aggaagtccc
    46921 ttctgcaaaa tcctgcattt cgatcaatcc tgttttgatt cctgatgctg ctgactggac
    46981 tgccacaccg gcaccagtga ttccgccacc gatgatcaac agatccaatt cttcttgctg
    47041 cattttttga atgctatctt gtcttgtctt gtttgaaaac atatggattt cctccttatt
    47101 caaatgcttg ggtagctttt actgcttttt tccaaccgtt gtaaagtttt tctcggcgct
    47161 cttcctccat ttgaacttca aatagcttgc cagcttcata aaattcgcgg atctcgtctg
    47221 tatctttcca ataaccgaca gctaatcctg ctaaaaatgc tgcccctagc gccgttgttt
    47281 ccaagttttc tgctcgttgg atcggtacat tcaagatatc tgtttggaat tgcatcagat
    47341 attcattgtt tgctgcaccg ccatctactt tcaatacagg aatcttgatt ccagtgtcat
    47401 cttgcatcgt atcgatgata tctctcactt gataagcaat cgactgtaaa gtagctttaa
    47461 tgatatcttc ttttgtcgta ccacgtgtca agccaaacat agcccctcgt gctgcctgat
    47521 cccaataagg agcacctagc ccaacgaatg caggaacaac ataaacctca tcttcgcttg
    47581 tagagcgttt cgctgcgtct tctgaatcgc tagctttttg caacatttga agtccatctc
    47641 tcagccattg aatcgaagaa ccagccacaa aaatacttcc ttctaaagca tagtaaacct
    47701 ttccatttat gccgtaacca attgtcgtca ataaattatt tttggataat tgcggttttt
    47761 caccggtatt catgacaata aatgaacctg taccataagt attcttgacc atgcctggtt
    47821 cgaatgccat ttgtccgaat aatgcggctt gctggtctcc tgccatccct gaaattggtg
    47881 cttcgcttcc ataaaaatga taaccttgcg tataaccata aacttctgag ttagaagtga
    47941 cttttggtaa catcactcga ggaatattta gtaaatctaa aatctcctga tcccaatcta
    48001 gctgatggat attgaacaac atcgtccgac tggcattcga gtaatcagta acatgtgctt
    48061 gtccatcggt cagtttccat actaaccaag aatcgatcgt tccaaacatc aattcgcctt
    48121 tttcagctcg ttcttgcgcg ccttctacat gatcaagaat ccagcgtatc ttcgttgctg
    48181 agaaataggc atcgatcact aaccctgttt tttcgtggaa aaattcttga tagccttcat
    48241 tttttaagtt atctgcaatt ccagctgact gtctggattg ccaaacaact gcatgataga
    48301 ttggacgtcc tgtatctttt tcccatatta ctgttgtttc acgctggttc gttatgccaa
    48361 tcccagcaat ctgatttggt ttgatccctg attcgatgaa cgctccggca ataactgact
    48421 gtacagaatt ccagatttca tttgcatcat gttccaccca actttcttgc gggaaatatt
    48481 gcgtgaactc tttttgagaa ctgccgatgt gtcttccttt cttgtcaaag ataattgctc
    48541 gtgagctagt tgtaccttgg tcaatcgcca tgatatacgt tgattctgtc attgtgattc
    48601 cctccattat atgcaaacgc tttctttaac tactcttatc atactactgt tttaaaaaaa
    48661 gaaactgtca aaattttatt ctttttttct ttttttgaca gttttttatt atgaagctat
    48721 tttttaatca attcttttct tttttcagcg tctctattct tcttttcaat cgaataatgt
    48781 cgtaaggtga cgcaaattct gacagataat attcacactg ttttttgttt ctctggcaaa
    48841 atgtgatcac caaatcatat tctttacctt cttcataact tccaatctca ataccgccga
    48901 ttcccctcag ttctctgatc agatattgtt gaaaagcaat acgataaatc ggcagacttt
    48961 ctaaatcgat tccaatcact actggttttg caatataaaa atcgatcatc aaaagaacat
    49021 tggcatactg gatcatcagg taactaagct cactatcttc agctcttttc ctcggcaatt
    49081 gctgcaccgc tgtttcctgt aatttcgcca gtaactgtgt taaggaatgc ccgagcattt
    49141 tctgctgctg ttccaaaagc cgatcacgct catagacttc gatctttcca acaaagaaaa
    49201 accattcatt gttgatctgg gaaatttggt agcctactgc cttttcttct ttgattttca
    49261 aacgacgagg acgataatgc cataacatcg tttcccgaat gtaagtatct aaaacagcgg
    49321 ttggtaactt tttgctccgt gtcaaatcat actgataaaa tatttcttcg tctactatcg
    49381 aaaaagaaac aaaaaagctg taaaacatca ttgtttcgta tacattcagt tctgctgctg
    49441 ttcgactaag atgcatcacg atgattggat cgattgcttg ataaagccgg tccgactgat
    49501 agagtaattt cttttctttc aaagttgcgt acgtactttt ttcattcaat agtctttttt
    49561 tcgtaatccc gagccagcaa gcaatctgtg cttctgctga tgccgtaaac gttgtttcta
    49621 atacacggtc caatcctaga atgaacggtc ttttttctgg tgtattttgc aaaaaaagcg
    49681 gtcgctgatc ttccgggata tattggaata gttcataata aaaatatcgt atctgtagct
    49741 cttctccgta taattgaccg tttttgattt gtaatccaaa ttcctctaag agctggttca
    49801 attctttgat ctttcgaaaa acagaagact cactgatatt aaacgctgtc gctaatcgaa
    49861 cgatggatac ttgctgatgt tctaataaat agacgagcat cttaaacttc aagctttcct
    49921 gcaataaaaa caatagaatt ttttccaaac ttacatcttc gtccaacaca agcagcgcct
    49981 gatactcatt tcgctgtact tctacttttt tttccatcat tcggccaatc aattcaatat
    50041 cttccaaata ttgatcaaac gaggcttttg acagttggac atgctctctc aaaccattga
    50101 tagaggctgt tcccccttgc agaacaaggt ttcgcaaaat caggatctgt aactgctctt
    50161 ttctttctaa taatgcttca atcttcatag gcagtattcg ccacctttga ccacaccata
    50221 tctggcacac ggtattctgg ctggaaaaat agctcatgtt cttctaactg ttcaaacgtc
    50281 catccttctt ctactgcaat tgtcagttgg tcaagaaggt ggaacatctc ttttttgctt
    50341 tggagctgtg ctccaagaat ctttttttca gaatcaaata gaaatttgag tttaactttg
    50401 ctttgattta ttgctgtaag cttgtagtct acgtatcctg tttcaatttt ttggggataa
    50461 aaaatgctct cttcttctgt caaacctacg cttcctaaga aatagccaaa ccactgattg
    50521 ccgactggac gataggttcc ctgatccttt gtctgtgagc cactaattgt cttgctgaca
    50581 tttcgtgctg tacggacagc gttatttact agagaaacat acattttttc atttgttgga
    50641 cgaaaagtta cttggatcgc atcgccaatc gcataaacgt ctttttgaga cgtttgcaag
    50701 tagtcatcta cccagatcgt tccatcatca ttcaatgtta attgttcttc ccacatctga
    50761 ttgtcggggc gagaatgatt agccagcaaa acaagatcat agttctcctt tcttttttcc
    50821 gtctctacaa tagcggtttc attttcggtc aaagacacaa cttgttcttc taaaaaaagt
    50881 gaaagccctt gatttttcat ttcctttact aacggttctg tcatttcagg atctagataa
    50941 cgaaaaagaa ttgactttcg gctttcgtaa agatggattt gtttttttct tgatgccaac
    51001 ccttcagcta tttcgattcc tgcttgtcct gcaccaataa ttgcaatttt ctttgcattc
    51061 atgatctttt gctgaagagc cgttacgttt tgtatctttt taactgggtg aataagcgtt
    51121 gattctgcag aaatatttcg aaacttttga tttgaaccag tcgctagaat caaacgatca
    51181 aaatccaatt tttttcctcc tgctaaaacg actttttttt ctttcaatcc tattactgtc
    51241 ttttctgtgt aaacatggat tcgatagcgg tcagtcagtt cttctttcgt gatccaagaa
    51301 agttgttttt cactggtaac ctttcctttt aatatcaaat tgaacccgct gggaatccat
    51361 cctaacttcg attgcttttc aatcaaatat atttcacttg ttggcatcaa ttggcgaaga
    51421 taaatagccg catgaatccc tgcaaacgac ccgccgacaa ttacaatgtt catctgcatt
    51481 cctctttctt ataaggtgtt ctactgctaa cttctcaatc agaaaacaca gtattcataa
    51541 ctcgctttca accttatgat acatagcgat gcagcagaat caaccctctc catcataccg
    51601 tgttctaggt ctgcttctca ataatgtact agtggaccta attgaagagt atcatttctg
    51661 aagagatgtt tcaaaaaatg tactcttata aaagaaaaaa actcgccttt tacgtacctc
    51721 tgtccaaata ggaaacagaa aagaacgaaa agacgagttt tagcgtgtgt aatgtaatag
    51781 ttagaataga cacatcattt atttttaatt ctttaatgct tcaacaactg cttcgcctaa
    51841 ttgatgagca gattgaggat tttgccctgt aagaagccgc ccatcaattt gaaccacttc
    51901 tgaaaaagct gctccagact gatagtttgc tcctttttct ttcaaagcat cttctgctaa
    51961 gaatggtact tcattcgttg taccatttgc ttcctcttct tcattactga acccagtcac
    52021 tgtacgtcca gctaaaatag attttccaga ctcatctgta actggcaaca atcctacaac
    52081 accgtgacaa acagctgaga ccagcccttc atttcgatag atttgtgaag cgactttccc
    52141 aagatctatt gcttctggaa aatcccacat tgttccgtgg ccaccagcat aataaaccgc
    52201 tgcataatca tttgcatcca catcatcagg acgtaaggta tttcccaagg cccgatttct
    52261 gaaatcttca tcttcataat aggcccaatc gatttcatcc atattcaaaa cactttgcgg
    52321 ttctaaaggt acatatccgc ctttaggact gacaaaatct gcatcgatcc cattttttac
    52381 taatacatca taaaaatgag tcagttcact taaccacaaa cctgtaggac gctcgatctt
    52441 ttcgtacttt tctacatttg ttaatacgat caatactttc attttcccat cctctttcta
    52501 taaaaattgc gttattgata acgtgctttc aagaaattat ataatgggct tggcaaataa
    52561 agctctttat atgcttcttc aaaaggtttc gttacaaatt catcctcacg gttttccaat
    52621 aatttctctg cccagtcagg atcgacgatc atttgctgtc caattgctgc aagttctgca
    52681 tatttcaata cctgctctac atcttcgcga ttgcggatcc caccgacacc aactaaaggg
    52741 atctttcctt gaatcgtgtt atggaccact tcaagaatcg atttatcgtt gtatttatca
    52801 gaacgagcta ttcgatcata catatttaat gaaacatgca gataatctag ttttgactca
    52861 cgtaatcgtt caagaagcca gatcgtgtct tcaaaacgga tacctggtgt ttcatattct
    52921 tctggtgaga aacgataccc tacgatgaat ggttttttag catattgttc aaccgctgag
    52981 aaaaccgttg ttaataattc ttcaataaaa cgataacgat tttccaagct accgccccat
    53041 tgatcctctc gtctattcga atgcggagag aaaaattgtt ggatcaaata cgtatttgct
    53101 ccatgcaact cgattccatc aaatcctgat tcaatcgctc tacgtgtggc tttgccaaat
    53161 gcttggattg tttcaacgat ttcttcttca ctcattgctc gtggtgtttc cgcatcagga
    53221 cgttttgctg caactgcact tgcagataca ggctgttctc cagataaagt tgctcgactt
    53281 gtcattcgtc caccgtggaa aatctgaata atcgcttttg cgccttctcc ttggattgct
    53341 ttcgctagct cacttaatcg aggaatcatt tcatcatgag cgatgctgag ttcacctggc
    53401 cagccttttc cgatatcttg gacatttgca gcaccagtaa taacagcacc aagaccttta
    53461 gaacgttgtt tataataagt gatctcatct tcggtgatca ctccattgaa aaagctgagt
    53521 tgtgtcgtca taggcgacag catcaatcgg ttcttcaatt gaactcctga aggtaaagta
    53581 attgattgat caaattgtga cattgtcatt cctcccataa ttcgctctga ttaacctatc
    53641 gagaatcatc tatcattaaa ccactcgatt agttgtacat gcaactaatt tgcttaaaaa
    53701 attttatttt ttttgtaact tatctgttgc atcattcatt gtctgggcta attctttact
    53761 tgcttcattt cccaaaactg ctttgtatga ctgatgaagt tcttcccagc taccatcgac
    53821 ttccttagca aattggcgtc cttgttctgt aagataaact aatgacaagc gaccttcgct
    53881 aactgttttg acgtatcctt tatattctaa tttttcaaca aaacgagcaa tcgttgatgg
    53941 ggcggaatag agcatttgtg caatatcttt ttgtggtatt ccatcttttt cttttaatat
    54001 caataaaata aaaccataag ttggtgccag acctgtcctt ctaaacgctt catcagctaa
    54061 tttattcaac atgcgatcta gctttttcac gctaaagaaa agacattcat ctaacgcttt
    54121 ttcaattggc ttttccattt catcccctcc attggcgtac atatcatagt atgattttta
    54181 ttttttgtca aaaaacacgg aataatgtcc gcctcctctg tctagatttg aaaaaaagaa
    54241 gtagccatct tcaaagaatt tcggcttgtt ttcgacaaaa ctccttctgg aacaccgtga
    54301 atcagaggtt gtgaaaaagt cgtttagttt tgagtatgaa gaaaaatttt gtgaaaatgg
    54361 ctttccacat ttttgcataa atttggctta ataccgaaaa actggctttt gaacaccgtt
    54421 tatatcagag gttgtgaaaa agccgtcctg catcaagtaa taagaacagc caaacaaaaa
    54481 tagttcctca tattttgtcg ttttttgagc taatgccgat ggtcagctga ttgtacaccg
    54541 tttattgaga ggtcgtgagc ctgccgttga actccgagtc acaaggagca atttcaaaaa
    54601 acagcttctc atgttttatg actatggtga cttgtgatcg aggagttggc aggagaatac
    54661 tgtttattcg taaagaaggg gctgtgacaa gacttttgtc acagtccctt atttcctttt
    54721 attataggat ttcgccattt gattggatga cttgtctgta ccaatcaaat gattttttct
    54781 ttaaccgttt ctttgtgcca ttcccttcgt catcttgatc aacatagata tatccataac
    54841 gttttgacat ttgtgaagta gaagcgctga cgatatcaat gcagccccac gacgtataac
    54901 caaataaatc tactccatct ttgatagctt ccttcatctg ctcaatatgc tgtttcaaat
    54961 agtcaatgcg gtaatcgtct tgaacggttc catcttcttc tactttgtct atagctccga
    55021 gtccgttttc agctatgaac agtggttttt gatacctatc atataattga ttcaaagcaa
    55081 ttctcaaacc cttaggatca atctgccagc cccattcact tgatggaaga tacggatttt
    55141 taattcctcc agttattaag tttccttgga ccccttccca attttctgga tgagcactga
    55201 ctgcactaga catgtagtag ctaaaagaaa caaaatcaac agggtaggct gctaagattt
    55261 cttcatcacc tacttctatt tttaagttga cacctttttc ttcaaacatt cgttgcgtat
    55321 acgctggata atatcctctt gcttgaacat cactaaagaa caaagaagtt ctttgctgct
    55381 caattgtttt ttgaatatca tccggatcac aagaatacgg aacaagaagc tgatagctga
    55441 tcatacaacc gacttgtgaa cctttgataa tttcatgaca agcttttgta gctaaagcac
    55501 ttgctataaa ctgatggtgt gcaccttggt atttcgcttg ttccaaatgg ggatggtttt
    55561 cttcaattac tcctacgcta acaaaagggt gatgttttac acagttgatc tcattgaaag
    55621 tcatccaata ttttacttta tttttatagc gtttgaatac tgtttcagcg aaacgaacgt
    55681 aaaactgaat aagctttcga ttaggccatc caccatattc tgtaaccaaa tataacggca
    55741 tttcataatg ggaaagagaa acgactggct caatcccata ttttagacat tcgtcaaaca
    55801 cttgatcata aaaaaataat cccgcttcat tgggtgtttc ttcatcacca tgaggaaaaa
    55861 ttcttgtcca tgcgatagac atccgatagc atttaaaacc catttcagca aataaacgga
    55921 tgtcttcttt aaagtgatga tagaaatcat ttccgtgtct tttaggataa taatacttgc
    55981 tgttgggatc ctgcgccgct tttacttgct gatgcgtcat catatgccat tgatctgtcc
    56041 atttttcttt aggtaaatcg ctgtcgaatg tatacacatc agacacagat aatccttttt
    56101 catctacatc ccatgcacct tcacattgat tggccgctgt cgctccgccc cataaaaatc
    56161 cttctggaaa ttttttcata tcgatccccc taaacttgct agttttcttg tttgattatt
    56221 tttcttatga ctagtaatga tatttttgaa tctactacta tttgtaacaa taaccattgt
    56281 ggtcggatca tatccctctt tgattaattc ttgcaaatct acttccatca ataaatcgcc
    56341 tttacgtact ttttttccat cagatactgc actttgaaag tatttgccat ttaaattcac
    56401 tgtatcaata ccgatatgga tcagtatctc tgctccttct tcactgacta ccccaattgc
    56461 atgtttcgtt ggaaaaactg ttgaaacttc cccgtttaca ggactgtaga tttttccgtc
    56521 cgttggaatg atcgcaaacc cttctcctaa agatccacta gcaaatgttg gatcgtttac
    56581 ttcagataaa tcaattattt cacctgctgc aggagtactt aacactatat cctttgagga
    56641 ttcttcttcg atttcatcct tgtagccgaa aagaaaagtt agcatacagg aactgatcat
    56701 cgctaagaaa taactagcaa ctagccaaac aaatccttca ccaatataaa tcgggaaaga
    56761 caaaatactt cttgtagcaa ctgccaaagc accggctcct gcggcaccag ctactccacc
    56821 aaatatagct ccacatatac aagcaatgta aaatggtcgt ttatatttaa gagtcacacc
    56881 ataaacagca ggttcagtga ttccaaataa tgctgtgata aatgcagata aagataaact
    56941 ttttactttc ttatttttcg ttttcaaaaa taccccaagt actgctcctg cttgcgcagc
    57001 tacggcagtc atacatgctg gacctagcgt atcacgaccg tagaaagaaa gattgttggt
    57061 cattactggt acaagtcccc aatgtaaccc aaaaatcact aacacttgcc aaaatccgcc
    57121 gataacagca ccagataaaa cagggctaaa gttataaaca gctaaatatc cagctgcaat
    57181 cagctgactt gcataatctg caattggacc tacaactaat aaagtaattg ggaccatcac
    57241 gatgatacaa atgaatggtg tcaataaatt tcgaatactt tcgtgcaagc ttttgcgaat
    57301 cttaggctca atataagaaa gaacccatac agctaaaatt gctggaataa ccgtacttgt
    57361 ataccttgct agaatcacgg gcatccctag aaatcgcaag gttatgctat cactatatgc
    57421 actgacaatc gtaggataaa cgagagctgc tgcaataacc atagcgatga aacgatctgt
    57481 attgaacttt tttgcagcag tatatgccaa aaatataggc aagaaataga aaaatgcatc
    57541 agctgctgca ttcaaaatga tataagttcc tgattcagta gttaataaat tcaaactcgt
    57601 acataaggcg agtactcctt tgatcaaacc acttcctgcc aagacgccta aagtcggggc
    57661 aaaaacagcg gcgatcatgt ctataaaaga gtttaaaatg ccttttttct cagttcctct
    57721 ctttccacta gatgcatcat cttctagttt catttgtttg gataatacgt catacacttc
    57781 atttactgca tttcctacta cgacttgaaa cattcctcca gcttcgacta cagtgatcac
    57841 accttctagt tgctctacct tctccttatc tgccttctta ttatctttta gggtaaagcg
    57901 taaccttgtt gcacaatggt aaacattgct gacattttct tcgcctccga caaatcggac
    57961 aatatctttc gctaattgtg aatagtccat ttttacttcc cccttgttaa ttaatgccgt
    58021 agcaatcctt cttacaagat aagtatagtt cttagaaaac ggtttctcca ttcaattagt
    58081 accccttata atagcgaacg aaactgtaca atagtgtgta tggtttactt agccgagcaa
    58141 atcactcact gcttgaaaga aaacaagtat atcgtttctc ttatagtcaa aggagacaaa
    58201 aaaaacacgc ttgatcttcc tttgctgatg tataagtggt atctcaagct gatactctgt
    58261 gatttttgtt tctacttggc ttttgataag acagcacaac aaagtctcgg catttaacgg
    58321 aagcataact aggtcagaac taggagagat tagttgctga atggaatcta tctttttatg
    58381 gatttttatt tgattttctt ctttgtagta atgatcaagc agttttgttt tctttttttc
    58441 ttcaactatg gtcagctttt cacgcaaaat ctgataaatc tcccaagatt caaactcatc
    58501 tagaacacta ggtagataaa tcaaatcatc ccactcaaaa ataagctctt gctcagtagt
    58561 caaaattgca tcatattccg ttattttgtt tgattgattt ttgaaaagat acactggttc
    58621 tatccggcat tctttgatcc acatgttcat actttgttga atctgttttt ttagtgtatt
    58681 caaaatacta attggctggt tactaacaat cagtatctcc ccttgttttc gaaaaggatt
    58741 caacgcttcg gcaagataca aaacaaaata agcgctttca gctaaattgg gacgtaattg
    58801 aaaatgtatt gggaagaaac gccgaattaa ataaatttct aaaggataac gggagatgat
    58861 cttttgtaca aaatgattca tggtattatg tccggcatcc atcctcgttt tcatttgttt
    58921 gatatgggtt agcaaaaatt ctgtttcatg gaacaataac gaaacaggaa gatctaaaaa
    58981 acgaataatc gtttgctcaa atgctcttag attcttctct atttctttgt ttttttcatc
    59041 attaatcatc aaataattta actctaatag tcgattttca attgcttgtt tttcttgatt
    59101 ggaaaaagaa tagggtagtt ctgatgataa cttttttgtg agggtctcta ccatcggaat
    59161 aaataatgta tacctgtttg ttttttctaa aagttcttct cttcgaagaa tggtcaaaac
    59221 gagataccga gaaaatcgta tgaagtcctc tcctgaaaca atataattat atgatataag
    59281 aacctctttt aatgtctttt ggacagtcgg catgatattg gaaaaaattg tactgatttg
    59341 ttgaggcaac cttgcgtgtt ggatcaccgt ttctgttcca acaagagata agaaaattct
    59401 ttttgaattt tcttcagcaa tgatttttag tccttttgtc gacgaaactt ccagttctaa
    59461 tttaggattg cgctcgatcc aacgcaaaat cgtttttatt tgtaatgata cggctgtttt
    59521 tgaaagaaag aaaatgtctg ctatcttttg catactgaca tacccttttt gaaacaataa
    59581 gtacgtcaca atagttgaag ctcggaaatc catgctggaa tagattttat gtttcgtcac
    59641 ctcttgtatg atttgctgtt tagtttcaga ttcaattgat tctaaaaaat aacctgaacg
    59701 tttatttgac tggataatga ttttcttctc gtagatagca agcatatttt tgatttcagt
    59761 ttggatcgtt ttcttgttgg tacctaaagc ttcagctaat tcttcgcttg ttatccaatg
    59821 attcttattt cttaatacta caagaatttg gagttgtcgt ttatttaagt ccatttgctt
    59881 acacctcctc tttgttataa acatacagga aaaacctctc aaatgacaat agtcacttgc
    59941 agaggtttaa aaatcatttg aagaaagatc tatattcaag gtttttacca ttagactgga
    60001 taatttgttt ataatattca aaactatctt ttttcaatct cctaaagctt ccgctcccat
    60061 cattttgtct atccacataa ataaagccat atcgcttcct catttcccct gttcctgccg
    60121 atacgatatc gattggtccc caccataagt aaccaatgat ttggcaacca tcttttattg
    60181 cctcagcaat ttcctgaaga tgtattttta gatactcaca acgttttgga tcgtggattc
    60241 gattcaattc atctggtgct tcatctagtc caatgccatt ttcaactatg aataatggta
    60301 attcataacg gtctgtcaat tcattcatga cataacgaaa ccctagagga tcaattggcc
    60361 aactccaagg ttcgggagat ttttccttca aaaagggatt atcgaaacca tttgctccgc
    60421 cagtatctac gcgcatttct gcttcttttt tatagacagc gctacgataa taactaaatg
    60481 caatataatc tgccgtgtta gcttgcaagc actgttcgtc tccatctaac attactggtg
    60541 cacagtcttc ttccttccat gtcctgtaaa tataagctgg ataatgccct cggcacatga
    60601 catcggagaa aaaccatgaa ttgcgtttaa attgcagtga accaaataca ttctctggat
    60661 cacagtcata tgcataagtc gctaatgcag aaaagctaca cataataccc atcttagcat
    60721 ttggatcgat tttctttaat aattgagtag cacgggcgtt cgctagaaac atatgatgac
    60781 aggcttgata aatcactttt tttgttagtg tagcaatggg ttcaagttta tctctagcag
    60841 ggaatgtaac caatccagca gctgcataag gcatacgaaa agcattattg atctcattaa
    60901 aggtcatcca atatttcact ttcccttggt agcgttcaaa gacggtctgc gcataaaatt
    60961 caaaaaagtc gattaatttt cgattttgcc accccccata ctctcctacc aatgctaagg
    61021 gggtttcata atgactaaga gtaattactg gctcgatatc atatcgattt aatgtattga
    61081 ttagtctgtc ataaaatacg agaccagcct catttggctt ctgttcgtct cctcttggaa
    61141 atattcgtgc ccatgagatg cttgttcgaa aagcttttaa acctaactcc ttcagtaaac
    61201 gaatatcctc ttcaaatcga tgataaaaat caatcgcttc atgagaaaga taatggagtg
    61261 attcgtctaa agcaatctcc caaatctttt tttcttcatt ccactgaata ctaggatgct
    61321 ggttcatgat cccgataagc gtatctgtca cattaggtaa tttgccgtct tccaaccatg
    61381 caccttcaca ttgattagca gctactgctc cgccccataa aaaattttct ggaaatattt
    61441 cctgatccat aactatctct cctcgctatc aacaatttct tacttgaagt atagctcgat
    61501 tcatcaaaac aagatattct aactatttcg gcagaagaga agaaaaaaat tgaatgctac
    61561 tcttcatttt taatatttat atataacaaa tacataagac gatccccgaa agaaaacact
    61621 cgttaaccat ctaagcaaac gatttatttt acttccaaca caaatgcttc ataaggtcgt
    61681 aaatcaactt tgccgtctgg taactgtttc agaggataat ttgacacaat aacccttaaa
    61741 gcttcacgtg gaacagtgaa cgtctgcttt tcactggaca tattacaaat aacgagccat
    61801 tgctggtctt catatatgcg agaataggca aagacatgct caggcgtctt ttccactaat
    61861 tcatagtctc cccagacgat gatgtcatgt tttctacgca aatcgatcaa tttcttgtac
    61921 atataaaaaa cagaatcttg atctgctaac gcttcttctg cattgatttc tttataattt
    61981 ggattgactc gaagccatgg aattccagta gtaaacccag catgttcacg ggcgttccat
    62041 tgcataggcg tcctagcatt gtctcttcct ttcgcattga ttgattccaa gatttcctct
    62101 tttgtaaatc cagaagaaat cctttcgtta tacatattga ttgtctcaat atcttttgct
    62161 tccgcgatat catcgattgg gcaattcgtc atgcctaact cttctccttg atagatataa
    62221 ggggtgcctt tcatcatatg gagcaagata gcaaacatct tggcactttc ttttcgatac
    62281 acatcgtcat ttccccaacg agaaacaatt cgcggaagat cgtgattatt ccaaaataaa
    62341 ctattccatc cttcatcacc taaagatgtt tgccatttgg ataatacttt tttcagttca
    62401 gagatactca agggtttcaa atcccatttg tctttgcctt cttgctgatc taatcctatg
    62461 tgttcgaatt ggaagaccat ggataattca tttcttttag gattggaata aagtttagcg
    62521 atttcggtag ttgctcccca tgtttcgcca acagtcatca gatttttgtc accaaaagtt
    62581 gcttgattca tttcctgaag atactcatgc agtttaggtc cattacttgt gatttcttta
    62641 tccggttctt tgccaatcat atcgatcacg tccatacgaa agcccccaat acctttatcg
    62701 atccagaaat tcatcatttg ataaatctct tggcgaacct gtttattttt ccaattcaaa
    62761 tcaggttgac gcttgctata caagtgcaga aaatattgcc ccgttttttc atccaactgc
    62821 caagcagaac cactgaatat tgagcgcaat ctattaggct cttttccatt gaccggatca
    62881 cgccaaatat aatagtcccg ataaggattt tctttatttt tacgggcctc aatgaaccac
    62941 ggatgttcat cagaagtatg attcaccacc agatccatga tgatacggat atttcttttc
    63001 tttgcttctt caatcagttc ttccatttct tccatcgtac cgaattctgc catgattgct
    63061 tgataattac tgatatcata cccattgtca tcattcggag actcgtatac tggagaaagc
    63121 caaattgcat caatacctaa atacgccaga taatccaacc tggaaataat cccccgcaaa
    63181 tctccaatac catcttgatt tgaatcttga aaacttcttg gatagatttg ataaacaact
    63241 gaatggtgcc accatttttg ttccatgatt ttcctcctat aaatggatgt ttctattttt
    63301 tgcttaatca tttattttag tctctctcac taaaaattgt aacgctttca aatagctatt
    63361 gcaccttgtt atcggtaaca gaataaaaca aaaaaacagc tgaggaaatt ccctcaactg
    63421 ttttttgaaa ggattgattt atgcagggaa gaatgctgat ttcacaactt ctttataaac
    63481 ggtgttcact cagctgtccc ttggtattag ctcaaaaaac ggcaaaatat gaggaactat
    63541 tttttcgttt ttttctctaa tactcaggcc agaacactga tttcacaacc tctttataaa
    63601 cggtgttcac tcagctgtcc ctcggtatta gctcaaaaaa cggcaaaata taagaaacta
    63661 ttttttcgtt tttttctcta atactcaggc cagaacgctg atttcacaac ctctttattt
    63721 cacattcaat atccacatga atcccacaaa tacaaagaac agaccataca tgattggaga
    63781 tacttctttt cctcttttag ctgctaacat ggtaattgga tagaagataa aacctaatgc
    63841 aatcccatct gaaatactat aagtcaaagg catgccgatc aagatcaaga atgaagggat
    63901 cgcaatctct aagtttcccc attcgatttg tcgcaaagat tgtgccatca atactcctac
    63961 cataatcaat gctggtgcag ttacttgtga agtaacaact gatagtaatg gagaaaagaa
    64021 caagcctaac aagaagaaaa tccctgtagt aatagctgtg atacctgaac gtcctccaac
    64081 ggcgatacca gctgatgatt ctacaaatgc accaacagga gaagtaccaa ataaggaacc
    64141 cgcaagcata gctgttgaat cagaagctaa agctttccct acacgaggca tcttgttgtc
    64201 tttcatgaaa cctgcttggt ttgctaatcc aactaaagtt cctgctgtat caaagaaagt
    64261 cacaagcaag aatgtcagca ctacgaccca catttgaaga gaattgatgt cccctacatg
    64321 ttttaatgca actaaaaatg ttggtttcaa acttggtgct gcggcaacaa tttcagaagg
    64381 cattggaatc aaacctgtga ccagacctaa aatcgttgta gctgctatcc cgatgaagat
    64441 gccacctggt acacgccgaa caagcaagat agcagttacg actaatccaa aaatcgtcaa
    64501 ccaagtagaa ccaacgttga gtgatcctaa tcctactaat gtagattcat tcgaaacgat
    64561 gatcccacct tcgctcagac caaggaaagc gataaataga ccgataccgc cagaaatcgc
    64621 atatttcaaa tctgctggga tcgcatcaat gatcatttcc cgcagcttga agattgtgat
    64681 caaaataaaa atcaaagaag caacgaatac gccggctaat gctgtttccc atggaatccc
    64741 cattcctaaa caaacagaat aagcaaaaaa agcattgatc ccaagagcag gtgccgttcc
    64801 aattggataa cgggctaata cccccattaa aacacagccg atcgcactgg ctaatgcagt
    64861 cgcagtaaac actgctcctt catccattcc agaagcacct aatacagttg gatttacaaa
    64921 taagatataa gccattgaga taaatgttgt aaatcctgcc agtacctcac gttttaccgt
    64981 ggtgttcaat ttttctattt cgaagtagga aagtattttt tccttcatct tccatccccc
    65041 taaatccgaa cattataaat aaagtccgtt aaaatttcga tttattaata aaacaacgtg
    65101 ttttatcata tactaaaaca aaacataagt aaacaaaaaa ttcggatttt aagcaaaatc
    65161 cgaacgttcc atctgattaa atccctttat ctcaaagttc atgaacattc actttggatt
    65221 acaagaattt ctttattgtg ttaataaagt ttcttcattc tagtctcaaa aatcaatagt
    65281 gtgatttgat tttaccattt cgtttctgtt cttctttgat gaattctttt gtatcgacag
    65341 gattcagatt atttagtccg ccaaaacctg tatcgaccat ttttccccag ccttttgtct
    65401 tcttttttcc tgatttgaga ttctttgctc gaagcttctc actatgtttt gctcgccgat
    65461 acttcaaata accttctaat cttcccatta gatccactgt ttttcctcct ttttattcaa
    65521 tcagacgact cagcttttcc tataacaaaa taattgcctt cagggtcaga aaaattgaaa
    65581 gtcaagatgc cattgttatc tgtgatttct cctggttctt ccatgttctc gtagatagtt
    65641 tcaaattcat ccgtaaaaaa tactaaagaa ggcggtggtc ctaatacctc tggtgaaaac
    65701 ttctgcacaa atacttttgg gaatacgcct aattctaatt cttttgagat gctgagtaca
    65761 atactcttaa attcttctgg caattcgatt gtttcagaaa tttctgcacc aattttttct
    65821 acaaagaatc ggcaaatcat ctctacgtca ttgacataaa gcatcacacg tgttttttgt
    65881 atcatcattt gacccttctt tctatttgat tttctttcct gtctataaac tgattccatt
    65941 atatcacgaa agcgcaaaca ccttgtgttt gatactattc aagaaaaata aagcaatagt
    66001 ttctaggatt gctttcctcc acaaactttt tttacaaaaa aaaaaaacgg caatgaatgc
    66061 cgctcttctt atttcgattc ttttataatt ctgcttctga ttcatctttg gttagtgtgt
    66121 gtctaacact agttacgcca aatgtttgat cgattttttc cttcaacgct tcgattgcct
    66181 gtgcgccacg tgccgcttgt tcagcattga tggattcaat gaccagcacc gctgcttgtg
    66241 tttcttccgt cagcattgtt ctttgggctt cacgccagtt gagacagcga cagatcgctc
    66301 cttgttcatc atagtagatg atttcttccg gaagtgccgg agcatcggaa tctgcaccta
    66361 aaggcaggaa tgattcgcca ccttgggctt ttcctaaatg cagatttcct gcgatctttt
    66421 cgatatcttc tccgccgcaa ggtacgccaa aagacaagga aatactatta tagatatcta
    66481 caagcggatt gattgggaaa aattcccgtt cttgatttgc acgtttcagt aaagcttcaa
    66541 tagaagaacg tgcacctttt ttagtcttga atgtggtaaa tgcctgacgc cactgagcaa
    66601 tgacttcgtt ttggctgaat gtttcttctg taaggtaatt tcttgcttct tttgctgcct
    66661 cattcaataa ttgatgatac gtttcttcac tttcttttgt tacgtggtta tcgataccat
    66721 ttacggataa gatttcgatg gttgcttcag gaaatacttc ccagaaattg gtatccacta
    66781 ttactttttt catctgttca cacctcttga ttagaataat tgcttgatga atagatagtc
    66841 tgtttgggga tcatctccca tataaaaaac atgttcgctg tatttgataa atcctttttt
    66901 acgataaaaa gcgagcgcaa catcgttgtg ttcccaaaca ccgagccaaa tggctgtttt
    66961 ttgcttttct tttgcttttt caatagctgt atctagtaaa aatgtaccga taccttgatg
    67021 tttaaatttt tttcgtacat agattcgctc gatttctaaa aaatgatcag ccatttcctc
    67081 agattgactc gttcctgtat ttagtttcag gtaacccgca ggctcttgat cgatactcac
    67141 taggaaaaaa tcggtttctt tatcttttag ctcttctgta agtttttcta gtgcataagc
    67201 ttgtgtcaag taatcagcta aatgctccgg tgaattatct tttccaaatg tatctgtaaa
    67261 tgtttcgata ctaatttgtt gtagtaagtc tacatctgaa ggcgtcattt ttcgaatttc
    67321 gtatttattc acggcttctt cctttcaata gatcctttta ttgccttttt tgacaaaatt
    67381 ccaatcttcg ctaacattct tgcatacttt atttaaaaga tattccaatt gttttgcttc
    67441 ttcgtcagaa agaccattta atgcgactgc attggagtag ttgttttctc gaatgatgaa
    67501 aggatagatt tctgctcctt ttttagtcgg atagagtttc ttgatttttt tattttcttt
    67561 atcttccaaa cgttcgatca tcccattgga ttctaatttc ttgattgctc gggcagtcgt
    67621 tgtacgatct actttgatca tttctgctag tttttcttgg atgattccag gattttcgca
    67681 aatacgtact aaataaagat actgtcctct tgttaaatcg acttctttga attcgatatt
    67741 gctaatagaa tctagcgctc gggcaatcat tccgatgtct cgaaggattt cagccatttc
    67801 atcccacctt tattttgttt gtaagactag tttacctctt tttgttgcaa atgcaactat
    67861 tttcaataaa aaaatcaagt tcttttttaa taactagtaa agtatattct tttcttcttt
    67921 tacgaaaaag ctcaatcaca atagatctgg tttttgtttt ctaaaataag taactgtttc
    67981 ttcgttgttc gcttttctga tagctttggc aactcttcta tactaaaaaa tcccatgttg
    68041 gaagtttcat tattctcaat aaacttggcc tcttcaccgt ggatcgcaca agcaaaaaca
    68101 agtttataat actgaaaaag ctgtggaata tctttttgtt tattcgtatc aaaaactgct
    68161 cgcaactgag tagtttctac tgttaatcct gtttcttcat agacttcttt acgcacattt
    68221 tcttcaggag acaatcctat ttcagcaaat ccaccaggaa gtgaccattc tttcgtccgt
    68281 ttatcttcga ttagtaggac cttgccatct tttttgatat aggcacgaac atctactttt
    68341 ggtgttggat gtccttctcc aatcgttgtg agcgctttta tttcttcgaa agtttctttt
    68401 cctatttcac tgatcagttc caatgaaatc gtccggatct cctcaaaacg ctctttatca
    68461 aatacatctt ttccataata tagaccagct tgagaaagtg cgagtagtct tttgtattga
    68521 gccaacttct tgttcattct cttcccttct tttttagcaa atgtatgact atgtaatcaa
    68581 gtatagaaga ctggtttaat tacggcaaat aaatataatg aaatctgcaa taaataaaag
    68641 aatgttaaaa aactcgagaa caaaagacat tcgacttctc tggggctgtg aataaatggc
    68701 gttcaaaagc ttgcatctgc ggtaataagc cccaccaaac gaaaaatatg acgagctatt
    68761 cttgtctggc tgttcttatt aattgaggca ggacagcttt ttcacaacct ccccactaaa
    68821 cggtgttctc ctaccaactc ctcggtcaca agtcacaata ttcatcaaac atgagaagct
    68881 gtttttttga aattgctcct tgtgactcgg agctgaacga caggctcacg acctctcggt
    68941 aaacagcgtt caaaagccaa tttttcgata ttaagccatt tctttgttac ttattgtcat
    69001 tgatttcttt ttctaattta tccaatagtg gagtgtaatc cacttgagta tagtctgtta
    69061 catcaaattc atatagtgtt cctaactgga atttgtcata ttttcggtcg gcgatgatct
    69121 ttttgaatgc ttctttcgta atatgattgg tccctttcgt tcgatcttcc aatacgtcac
    69181 catagtgata atgatccatg atatagctca aatgacgatc tgattcacga tcacgtttgt
    69241 accgacgttg acataacact tcaaagtctg ctgttaggcg aatcgtgatc acctgatatc
    69301 cgtatctatc tgctaattct tggagtcgtg ctttttgctt atcactaaaa ggatactcgg
    69361 acacaatcac tttctttcct gcttccatat acaaatcaag tgctttgtaa tagaaaggat
    69421 aaactttcgt cctttcttgt tccgcccgtt cttccaaatt gttaaacccg atggattctg
    69481 catagtattc cttgatttca tcgagcgtga tggcatacat atctggaaat cgttcccgta
    69541 gtttattcat aagatatgtt tttcctgtac caggactacc agccagaaga atgaaatact
    69601 ttttcatctc atttatttcc taagttaatt ggactttcca agttcataaa cagtccaatc
    69661 ttatgcttga caatttcttt tactgcttca tttgctggaa tcacattatg gcgaagagac
    69721 ttgttgattt ctgtttcact aaaggtttct ttagctactt tcgtccagtt gactaaaagc
    69781 tccgttccaa cattgaattt gcggatcccg ttattgatca attcaggata atcttcttct
    69841 ttcacacctg ttcctccatg gataactagg gggacatcaa ctactgcatc gatttctttc
    69901 agcaagggaa tgtttacttc tgtttttgat ttgaattgtc catgattcgt accgatagca
    69961 atagctaatg catccacgcc tgtagcttct acaaatttca ctgcgtcttc cggtcttgta
    70021 tataccttgt cattttcagc gacatggatt ccttcttctg taccgccgat tgttccaagt
    70081 tctccttcta cggaaacacc gtgcgcatgg gcatattcta ctactgcttt tgtcttcagg
    70141 atattttctt tgaaaggcag atgggaccca tcatacatga cagaagtata tccgctgtcg
    70201 attgcttctt tgatatcatc aaagtctcgg gcgtggtcta agtgcaagac tgcatcaacc
    70261 atttcttctt ctgcaatagc tgtcattaca ttgactaaca catgcatgcc gatatattta
    70321 gcggtatcga cacttgtttg aataatgatc ggcgaaccca tttcttttgc tgcgcctagc
    70381 atagcaggaa gcatttctag gttatgcgta ttaaaagctc caactgtaaa attcaactct
    70441 tctgctgttt ttgttacttc tttcaatgtt gtatacatct tttattgcca ccttatcttt
    70501 ttatttgttt cctttgatcg ttacttgttt tgcaatttgt cgcatctcat ccattttgcc
    70561 catgtagtaa tcgatccctt gatcatcacc agcacgagca gcatctgcac gcttctggtt
    70621 atagaggctc atcagttttt tatacccatc tccgatcgtc tgtttatttt ccaaactctt
    70681 ttctaaatat tggatggctt gatcgttcac atacaattca gaaaatttaa gtccaacttg
    70741 ttcataaagt ttcgctaatt tatgcttatc ttgggtagtg gaaatctctt gtttcaattg
    70801 tgtaatctct gattgaatct tttcggtcgc ttctggcgat agggcttttt cttcttttgc
    70861 cagaactttt ttcggttctt tttctttttt cttaaaaaat ccaaacatca ttttccctac
    70921 tttcaattaa aatgctttca aaactgggct taagatccag gcatataaga gtgctgcggc
    70981 tactgtatct acatcggttg ctgtagcatt gatgaatccc atcgaattaa agatcgttac
    71041 tagcaaagcg ggcaataatg taataaagaa accatgagca ataccgccaa tgatcgctcc
    71101 tcggcgtccg ccaactgcgt tgccaaatat ccctgctgtc cctccggcaa agaaattggt
    71161 tagcatacca ggtaaaatca tcgctaagcc gaacatgggc aaagtaaaca tggcaataat
    71221 cgtaccaatc gttgtcgtaa tgaaacctaa aatgacagca tttgggctat aaggaaagaa
    71281 aactggacaa tctaaggcag ggatcgcatc aggaactaat ttcatagcta tccctcgaaa
    71341 tgccggcacg atttcaccta acagtagacg tacaccagct agcaatacat acacacccac
    71401 tacgaattgg atggcttgta aaaaggcaaa catgatatag ttttgatctc ctgataattc
    71461 agaagcgaat ggttcgcctg caaataaaac agtgatgata tatagcggga ccatcacgac
    71521 catgacagac aaataagtat cttgcagaaa ttcgaaatgt gtcggcaact tgatatcttc
    71581 gatcgacttt ttattttcgc ctttctcacc aaaaagctta gcaacaccgg cttcaaataa
    71641 gtacccgatc gtacaaaaat gacctaaagc aatatcattt gatccagtga ttttacgaat
    71701 gatcggttgc gctactgccg gcatagcaat ggcgaaacaa gctccgataa aaccaccgac
    71761 caagatcaat acgatccctc gcagaccggc aaaatagcca aagactgtcg tcatcgttgc
    71821 catccataaa attgcttgac cagttaaaaa aatgtacttc cattttgtga aacgagccaa
    71881 aataatattg aatacaaaaa tggctaagaa cgtcagtgca atatctctac ctaaacccaa
    71941 atcattcatt gcttgaccat tgatagattc aatagaaggg atgatccctt gcatatgaaa
    72001 tccttcagta aagattttcc cgaaataagt taaacttcca acgatgatgc tcgaaccggc
    72061 actcaacact tgaaatccta ggagtgtctt taacgtgcca gagatgactt gtcctgctga
    72121 tttcttctgc agtagtagac ccaacatagc aatcaatgca atggtgatcg atgcctgagt
    72181 caaaatattt tcgatgataa aattgataac gcccatgttt ttaccctctt ttctttttgt
    72241 atttagataa ctcccttatc ttgcagaact ggggtgattt tttcttcaat ttcatttttt
    72301 gaaacaatat ttttcaaata gatcatcgcc gttttaggat caactgcgaa cttttcgaac
    72361 tgcgaacgaa aattttctgc tgtgatgatc acatctggtt tcatggaaac tgcggaagaa
    72421 atatcacaat ggtccagttt ggcttctact cctaatttgt tcaaaacatc ctcagcagac
    72481 atctgtgctg caaaactact tcctaaacct gcaccgcata cgaataaaat gttcaatttg
    72541 ttcatctttg ttctcccccg tatcatttat tatttctcaa ctactgaaaa aagcagttgt
    72601 ttgaaatctt cgatatttgt gcattcaatc aatcgatcca acttctcttc gttgttgatc
    72661 aattcaatta gttctttcat gatgttcaga tgcgagtagg agtctactgc tgccaaacaa
    72721 aaaataatct ttactggatc catttcagga ttaccgaaat cgattggttg ctcaatcgta
    72781 gctacgctga ctcctaattt attgacccca tcttctggtc gggcatgggc tagtgccatg
    72841 tgtttgccaa tcacgatata cggtccgtaa tcattcactg cacgaatcat cgcttcgaca
    72901 tagcgctctt cgacaatgtt ttctttaacc aatggtctag cagctagttc aatcgattcc
    72961 tcccaagtag tggctttttc tttgatcatg atattgctgt ccgtcaaaat atcttttaac
    73021 ataggctgga tctccctttt gttgatttct aaattgtttg tttcgaataa tttttctaac
    73081 ttgccataga tgattcgatc tactcggcca ccactttctt tcatgacatc tattatttga
    73141 gtgaataact ccgtcgattc atgactgtta ggctctaacc gctgtagttc ttcatttctt
    73201 ttcaaaaagt cttctataac caagatattc gtctcagtta aaatcggatc gacaactaaa
    73261 agcggcttat ttcgataatt caattgaaag gtgctgaaaa ccagattgac atccaattta
    73321 tctactaatt cgatttcttt tgaacttaaa actgcagtaa tctcgatctg aggaaacttt
    73381 tctttcagat tctctgctaa cagatttcca gtagccatcc catggttaca gacaacgact
    73441 gatttataaa cataattcaa gtctcgcttg attacactgg cgatcgtcga aaagtggatg
    73501 acaagaaaag ctatctcgtc ctccagtatc ttgccaccaa ttacttcttc gatcgtagga
    73561 acaaaatgag tgactgcgcg gtagattggg aaataatttg ttcggatgtt ttctttcaat
    73621 ggattcatga cttggatatg attcttgatt cttacaatca gtgcagccat gtgcttatag
    73681 agattttcga ataaagcttc ttcctttaga tggaaaggta cgtgtgtctc gttctctaca
    73741 aattggatca attggatcgt tagtaattga gcttgtaccc actcgattga attggagata
    73801 tctctagcat tgaacgtctc aatagtgaaa cgaagataat ttctttctac ttgcgggaca
    73861 ttgtccaatt gaaattcctc gatcatgtgg tcaataaatg cagaaaagct actgtctttt
    73921 atttctgtat ttttccaatt aatggcagag ataaattcat gtttcctgat tcgctggatc
    73981 cagatcaatg taaaaagcag gatttgtttt ttataaatat catcttcttg tttcaacaac
    74041 ttattggaat agatctcctg tatttttttg atttccacca aaggaaaata atggagaaag
    74101 attttttgag ccggtgttaa ttgtgcatct tgattcataa agaagtcggt cattcccaga
    74161 ttctgattga taaaatcata gatcatcgta cggatagatc gttctgaacc tttataaacc
    74221 agtccttgct ttccataact gacgatctct atcccataac tgaccagtgt tgctcgtaac
    74281 cgtctcatat cttcatctaa tgtactttta gagatttgaa attcttcttc ttttttgttt
    74341 agatggatcg ggattcggct gtaagcaacg gctaaaacta gatcaaaaat acgttcgcgc
    74401 cgattcaaaa agccgtcttt cacatctttt atttcagttt ctaatcgagc ttgcttttcc
    74461 ttttccagct tcaacaaaaa aactttgttg cgaattgtac ggatctccgg aagcttttta
    74521 ctgctcaacc agatattcgc ttcaagaact tcctgacgga tcgtccgctg actgacattg
    74581 aactcacttg ctagatgttt tgcttttact ggatattggg ctgtcatgat tctgtgaagt
    74641 atttcaattg ttcttgtttt catagaaatg atctccttct gatttcaagt atatggttcc
    74701 gcttacataa taaaaagtag atatttctgc cacttgaagt ggaaagaaag taagcaaagc
    74761 aaacaaaaaa taagccatat gctttttcat ctcctcaacg aaaaagacat acgcctatta
    74821 ttacaaaaat tttgcccatc tatacaaaaa agaggttgtg aaaaagctgt cctgcatcaa
    74881 gtaataagaa caactaaact aaaatagtgc ttcctatttt tcgtttggtt gttcttatta
    74941 tcgcagatgc aagcttttaa acaccattta gtggagaggt tgtgacagag atgttcagcc
    75001 ttgagcgata agaagcgatt ttgaaaaatc gcttcccata ttttacaaaa tcgtgcctta
    75061 ttgccgatag gctatctctg gcacactgtt tattcagaaa aaggagctgc gacaaggctt
    75121 ttgtcacaac tccgttcata aatatttcct gtcaatccag cataatcggc tgaataaaat
    75181 cggcttgcca tcttttttct ttctcagaaa aatcaaaata ccatatctga gaaaaatact
    75241 gtcgttgtgt cttacttcct gagatcaatc gcccatcttt tcgatcgact gaataatcac
    75301 agcatctgaa atcaattcgg atggcaaaac tattttcgtg gatctgtcga taatttccta
    75361 cttcctgaat agttattttt ttagtatgat ttcttacacc gtctgctttg tttttttgca
    75421 gaactttctg atgttcggca aacagcttgt ctgtataaca gcttctggct ttttcaggag
    75481 tttcttcttc ccaagcggat tggatcttca tgaatatctt ttggatttcc agaaactgtg
    75541 ctttcttttc tttagccgtt cctgacatgc gggacaatat ctccgctttc agccttttct
    75601 tttgtctttt attcttgacg atagaaaaag cagaaacccc tcctactgac aaaacagcta
    75661 atgcaagaaa tcccgtttgc tcataactag aaggacctgt acctggacca taatacccat
    75721 aagaatctct gccgtaagaa cgactgccaa aataactgcc cccatatgaa cgaccaccaa
    75781 atgaacctct gcccgaactt cgaccgctag atgtacctcc accatgacca cccgcgacag
    75841 cgaatccttg ggtcggcaga gcaaacaaac ataggcccac taacaaaatg attgctaaat
    75901 aattgatttt tttcattgtc ttcctcgtta tcttttatta caaaaaacta tttttatgat
    75961 agccttgcta aaaggaagag tcaataacca gatgcataaa ctgttttttt agcaattttg
    76021 tcttatttag tcattgagta acatcggaca aatgtatgtg tcggcagtcc ataattcctc
    76081 tatcttcagc atgaacaagc ttgcagacaa gagatctgcg cttcctccag gagagatttg
    76141 gttttcctga caaaagtgca acagtgctgt aaatggacga tttggtaaag cactttgact
    76201 agcagcatga gcaagttttt gcatttccac taacgttttc tgatcactgc gatgcaatac
    76261 gcatgtgtca tctagcgaac tcatcaaaac taacaacata tgcatttgtt tctgtacata
    76321 tgaatcatta tctagctcct tagctgcttg gattgcaagc ctgatatgcg gataccctaa
    76381 tatcgcttct tcatatgcac catttacgcc atatttttgc ttcaccctat gaccatgagt
    76441 ggttttttta tgacgatatt ttgtatcagg aaaagcagca agcttgctga catcatcaag
    76501 tattttcgat aaagaaaaag gctgttccat actgattttt tgtgcaatga cactgatcaa
    76561 tagccccatt gcccaaatcg ctcctttatg ggtattgatg ccatttgtag caatcagcat
    76621 tgccttttcc gcttttcgcc caatctcagc aattctttct cttaactcct gatcaatagc
    76681 atgatgccat gctatttgag ccatttcttc aaaaaaagga gttagcgtct tagcagactt
    76741 aagaaataaa gcatagtcca tatcggaatg tgcacccgca tccagtgcat ccactaatcc
    76801 aggttttgga cttaatgtta cttctgcgat caatgcttcc tctaccaacg cagcgagttg
    76861 ctgagtcact gctttttttc gttcgtcatt tatcatgaca ctcactcctt cttcctacta
    76921 ttacttgctg aaaccttttt tgatagctac ccaaatgata tgaagtaacc agcttaaagc
    76981 aaatgcacaa cttgccgtca ttagataagc tacaagaatc atgactcctg aaggtaactg
    77041 cagccaacta tctttaaaca ataataacag tacttggaaa acaaatacat tggctaaata
    77101 cgtacgataa gcataaacag acaaccattt gaacaatggc aattttggcg aatcgtttac
    77161 aatcattttg gaagcgccta tgaagacagc aaagatgatg actaaactgt ataaactttg
    77221 gatcgtattc aagtaaggtg cattcgcaaa gctcaagtct cctggatgtg cgagcaatga
    77281 acggacagaa aagaacgcga taactaaggc aacaggtaag aaaagataac gtactctgtc
    77341 caaatattga tagacttttt cgtgataaac aaatgcggct accccataaa tcatgaaact
    77401 agcaacaaac cgatctaata agtaccagct gtcatgataa ggcccagtca atacttgtgt
    77461 ttgatagaac agataccatg cgacatataa aatcgttgaa ccaatcaata ctggccatac
    77521 atatttttta ttagcaaata ctttgtaacc gagccaaaca aaaaacggca tgaacaattg
    77581 gatctgcagc atcataacgg tataccataa atgaggcgct ccatcacctg tgacatattt
    77641 cagtaaaaag cttgcagtat ccgtatacgg agtttgttgt tggactgctg gaaatagcca
    77701 aagataagcc gtcgtccata gaagatacgg aacgactagt tcaaaaaact tttctttcaa
    77761 aaattcccaa taagacaaat gctgacttgt ttttaccata ttataaacaa ttgcaaaaat
    77821 aaacattggt gctgtgtatt taacgatgac gtaaaaggta ccaagaaaag ctgtatttgt
    77881 tgcgctgtgg ctgtttgcca aagcaaagct caatatcgtt tgcatcataa cagccgttac
    77941 agctgatact ttctggaagt cacttacgtt gattttgatt tttttgtttt caaacatctc
    78001 tttctctctc tttttcttta ttaccaaagt tctttggtta atttaggtcc ttcgactgtt
    78061 ttcaatagaa tttttttctc tggctctctc aaatactctt ttagtgaaaa accgccttgc
    78121 cgagtcacta cttgggtatc gaattgctca aaaaaagcag gatgcgattg aattacctga
    78181 attggcaatt caatcggtga atttgcgtac agcagcaaat caaaatcact ggtttctttt
    78241 actgccggaa tcccagtcga taattcaaat cctaagctgc cgccgattcc ccatttacat
    78301 cctgataata tgtttctagc agcatgaaat tgctgataaa caggataatc catgattttc
    78361 tcttgtctat acgattcttg ttcgatgatg tcccacggat gaatgacttg tttgacggca
    78421 tgttttgaga tttctgttga ccatctctga ttgcgggcag gtccacgtaa accaatcggt
    78481 agtatttctg cgtgatccgt cgacgcacga cgaactgttc cgaacatctt ttcagttaga
    78541 acgacccatt caggcaaggt gaacccttct atttcatgtt tatcaaaaag gacaatatca
    78601 tgtgcttgaa tcatttttta tgcatcccat tcttcgtcca ttttggcacg gactttattt
    78661 gtttcgacac gtccgaatgt tttggctgtt tcgtttgtgt atcgatttgt cagcatcgtg
    78721 tcatttgtat tgcggacatc ttgaatggct tcattgattg cttcgatgac tttgctgaca
    78781 ttttgttctg aagcagcttg gtcggtgata tcttctagga aatgatagat cgctcctagt
    78841 tttgtataat tattgatatc gtaagcgata gctggaactt tttcagcagc tgcttcgatt
    78901 tcagcaaccg aacgttttgt cacacgtgca gcactttctt tagacatcgc ttggacggta
    78961 atttctttat tgtttaaagc aatcaagcgg cttgattgta atccatgtgc taaaaatgca
    79021 cctgaaatag catttcctgc aatcaatcca ataactggat gtccagcttg acggagtttt
    79081 gcataagcag cagcactatt agccaaagcc acatggatac cgatcaattc ttcgttatag
    79141 ccgtatgctt ggcttggtac atcgatcaca acgatgattg gccgtttcac ttttttgtct
    79201 ttatcttctt cgtaaacgtg atccaaaatt tgtcccatgc ggaaaccttc aactaaacca
    79261 acttcaccgt ttcgtacacg gtacatcggg tttttaggat caggtacgat tgatgtcacg
    79321 atggcttctt ctatgaattt acttgattta ccataataca cagtactaat agaagaagtt
    79381 ggatttttca tgccagttag tgccgcaaac caatcgtaac caacacttgc tgcagctggt
    79441 tcatttcctt ctgctacttc tggtaagtca attgattttg cttgatggtt agcaattgct
    79501 tcattgtaat cgtcaatggc cattggtttt gaaagatcta aagaatcaag taatgataaa
    79561 taaaatgctg agctttcact gcgatgcgtg tcttttcgtt cgtccattgc tgcgatgaca
    79621 gcagctttga ttgcttctac actatcagtc actaattcat caatgatttt tgattctacc
    79681 cgttgacgag atcctaatgt attccaaatc aagtctttgt cactagaatc aaattctcga
    79741 acgcctgctt cttgttcaat aacttctggt ccattcaaac cgactctcgc tttttttgtt
    79801 gcgattaaat aactcattaa tgctgaagta atcgacatac cgccaaatga tcctacacgt
    79861 cctggaatca aaccgatgac tggtacatat tgttttagag caatgattac atttccgatt
    79921 tctgaaatag ataacagtcc gtagtttgct tcttgcaaac gaacaccacc tgtatctagt
    79981 acaatgactg gaaagatctc atttcctttt ttattttctt ctaacacatg ttcaagtgca
    80041 gcaacgattt ttgcaccgga aacttcaccg ataccgccac cttggaattt tccttccata
    80101 gcgattacta ctgcttgttt gccattgatc gtgccacggc tgacaacgat cccgtcatca
    80161 ctttcaggga cgattccttg tgctggtaaa cttggcgcga tgatttgatc gaatggatca
    80221 atcaattcac gtccattgtc aagtagagcg ttcactcgtt ctctggcgtt taattctaca
    80281 aaactatttt tcatctgaaa gttcctccat tgcttgcgtc aatcgtaggt acacaactcc
    80341 tggtgtagca ccgaagtcat tgatcacgat atctgctgaa attggataac gatcaaaaaa
    80401 tctcgttaat acattgttcc atacttcgcc gaagccatcg cttcctgtac aaatttttac
    80461 agtagattct gttttttctg ttggaaacat caagatttcc aaatctcctg aaccgaccac
    80521 tcctacatgg attttcttat tgatcggctg tttcgttgga tatttataag ctaattgttc
    80581 catggatctc tccccttttt gtaaaacaga gcatacacgt tctgcttatt cgtctttctt
    80641 cttccatctg ggtaaatggc gacaaaaggt ttttctcgcc aattttatga ccaagttctg
    80701 aatttcattg gtggttcata caggccatct gaccattcga tcaaatcatc gatggtttgt
    80761 gcagctaata atgaacgttt cgcttcacta cgtttgatgc ctagatcttc tggtaatttc
    80821 acgatgccgg ctgcacgcaa gctatctagt gattctttcg tgctagtcat tccaagaggt
    80881 gtaacaccgc caattgctgc aattgctgct tgtcgttctt cttttgtttt tgctttgtac
    80941 aaataagcaa ttccttcttc tgtcacgata tgtgtagtat cttcgctata aatcatcact
    81001 ggtgcattgg ctaagcctgc ttgtttttga acagctagtg catctaattc ttcgacaaag
    81061 actggttttt tatttgaacc atatgtttca accatttgaa caactaattt ttggcctttc
    81121 cctaatggat catcattgtc gcgaagtgat tgccatgctg gtgtagaatg acgacgaccg
    81181 ccaggattat gtcccatgtt aggcgcaccg ccaaaaccag ataaacgtcc ttttgttacg
    81241 gtagaagagt ttccttcgta atctaattgc aaggttgaac cgataaacat atcgatggca
    81301 tattgtcctg cagcttgtga taatgttcgg ttactgcgca tcgtgccgtc tttaccgaca
    81361 aaaaagacat ctgggcgagc agcaatatat ttttccatac caacttcgcc accaaaacta
    81421 tggatgcttt caacccaacc tgattcgatg gctggaatca tcgttggatg tgggttcaat
    81481 acccagttct tcgcgatttt cccttttaac cctagtgatt caccataagt tggcagtaac
    81541 aattcgatag ctgctgtatt gaatccaata ccgtgattca aagattgtac gccgtgttct
    81601 gcgtagattc ctttgattgc catcattgcc attagaattt gcaattctgt gatgttttga
    81661 ggatctcgtg tgaataaagc ttctaattga taaggtttat ctgctttcac gacaacgtct
    81721 acccagtcac cagggatatc tacacgtggt aaagaatcga caacttcgtt gacttgaacg
    81781 atcacgatac catctttgaa agctgcagct tcgatgattg ttggtgtttc ttccgtattg
    81841 aaacctgtgt acaagttgcc gtctttatct gctttatctg cagctactaa aacaacattt
    81901 ggaatcaaat cgatgaataa gcgtccataa agttccaaat aagtatggat atcacctaat
    81961 ttcattgtgc cgtcttcaat catttgggcg acacgcaagc tttgtgcacc agcataagag
    82021 aaatcgatct tgcttgcgat tcctttttca aaaatattca aatgttctgg tcgtgagata
    82081 ctagacatga tcaaatgcag atcattcact ttttctgcat ctactttttc aagactttct
    82141 gaaagaaaac ttgcttgttt ttgattgtcg ccttctaata caactttgtc gccgggagca
    82201 atcagttttt ccattacttc tacgattttt tcagtttcta catacttgcc attgagcaag
    82261 ttgctgattt tttccaatcg ctgcgctttt ttttcgcgtt tagaagccca gttgcggttt
    82321 ggtttttcca taattagtca tctccatttt tgatatagaa acgtggcgtc atcaatacca
    82381 tgttggtcta gattgatcgt acggatccct ttatctacac caaattttgt atgaatcagc
    82441 tttgtcaaag tattgccggg gggaaattca atcgtcagat ccattcctaa ttcttttgcc
    82501 aagtccatca tttggttcca ctgcacaggg aaagctaggt tttccaacaa atctttacga
    82561 atctcttctg cgtcctgagt ggctcttcca tccgtatttt ttatatacaa acatctagca
    82621 tcagatagat ggacagactg catatacggc ttgaatttct tgaccgtatc attcatcaag
    82681 agacagtgag atggcacagg gactttcagc agcttcgctg tttgcgcttg gtgccgtttg
    82741 gcttctgcca acgtaacgga taaaccttcg atactcccag ataacgtatg ttgcatcgga
    82801 caattttcat ttgacagata aactggatgg tcaggatcaa atgtttttgc tacaatttcc
    82861 tcagcttcgc ttctcgtcac acctactact acgcccattg cataaccgct tggataagct
    82921 tctttcatta agactgcgcg gtgatagact agttccgctg cattctcaaa tctcaaagat
    82981 cgacaagcaa cagctgcaca aaaagcacca atcgaatgtc ctgctgcaat atcaggctga
    83041 ataccgtttt tttccatttc atctaaataa aatactgctt ttgtcagtaa tgctagctgg
    83101 ataaaaaccg tatcttgata gttttcttct ttatcaacta accgataccc cgtcacattt
    83161 tctaaacgtt ccatgtacat ttcagggata tccgctaaca tccctaactt ctgactgcct
    83221 tgtccaggaa ataagaaacc tgtactcatg agcctcgcct cctcagcact tactttttcc
    83281 actgctatct tagtcaattt ctgctataat aaaaaatgcc aaaaaaagaa aaaaccataa
    83341 caaaaaggta tggatgggat ggataattat gaaattgctt catctgcagt attttaaaaa
    83401 agtattcgaa acaaaaaatg ttacccaagc agccaaagaa ttatttattt ctcaacctgc
    83461 cttgagccgt gcgatcaaac atttggaaaa cgaattaggt gtccctttgt tttaccatag
    83521 cggcagaaac attgagacga ccgtttatgc agaagaattt tatccatatg cagtcaagac
    83581 attagacaca ttagaagaag gaatcaaatt attgtcttcg gtcaatgaga agatcgttac
    83641 ttccgtcata ttgtatttag aggtagcctc tgtttctatt ccgaacttgg tccgaatttt
    83701 tatggaaaag catcctgata tccaattaac gatcatgcaa catcatttac cagatgatat
    83761 taccgatcct gtactgtaca tcacttcgga gatgaagcct ggcatgacca atattcctat
    83821 catgaaagaa cctgtttatg tcgccattcc taagaaacat cctcttgcta aaaaagaaca
    83881 tctgcaatta gaagatatcc aacgcacccc catcttgatg ctgtcaaaga gcaacgcctt
    83941 ccgtcataca ctggatgcag cacttgaagc gaaaagtatc gaattgacga tcagttcgac
    84001 gacagatgat cctgcgacat tacgttctat tgtcaagcaa ggattagctg ttagcttttt
    84061 cccaaaagtt tcttggtctt acgataaaaa tgatcctttc gtcttgcggg aaatcaaaga
    84121 tttgcccttg acacgaacaa tttatttatc ttctagcttc acagaagatc atcctttgat
    84181 caaaatgatt gcaaaaacga tgcgggaatt ttatttagga aaataaaaaa aaaagagctg
    84241 ggacaaaatt cttgtcgcag ctcttttttc gaacaaacgg tgttccagaa gtaatttctt
    84301 agaaaataag ccgaaatttc ccaaaaattt gagaagcaat tttcgtaaat tccttcttat
    84361 ttcttgaagc taaacatttc tgtcacaacc tcttctctat tgtcctagac attcattgcg
    84421 ttgatttaaa acttccatca atcaaatctg ctagttcatc tagcttttct attttacgta
    84481 gatcaataga gatccaatgt tctttattca tgtgataggc tggaaaatac ccctcttttc
    84541 ctcgcaaaat tccgccaagt tcaggatcga ccttcaaatc aagaatattg atgatcctat
    84601 ggtcacttgc atcatcatag attttttctt ctggaacaga catcaggacc ccaaaccatt
    84661 ttttgctttt ttgatgtctg aatacgcaat aatcaggaaa ttttttaaaa atatattctg
    84721 gagacaaatt gtatgtttca ttgatgtgat cgattagttc ttttttcgtt agcatctgct
    84781 tccatctctt tcttattgat tcgctttatt caaagatacc acgtaagaat agaaaaatga
    84841 atacaagtag ttgctaatca aatacttcga tatttttctt ttttatcaac tcttaaatgt
    84901 tcaatccgcc tgtgttggtc ttgctaccat ctactattaa aatgtttatt attgtttgcc
    84961 cacctatttt ttgattttag tggtatcatt tttcaagttc atgatgaaat tcttatcatc
    85021 agtaaatgct ccagaaaaat aaacaatgat tgaaaaatca gttgaaactt gaccaccgtt
    85081 tattttctcc gtattcttag aatagaaggg agtctgattg atgtattcac taatgaaaaa
    85141 aatcattacc gaaaaagata ttcgacgtca tgtttcttta gtagaacaat tgctgaatca
    85201 tacaaagata accgtaaatg aattagctga aatcataggg acaaccgaac gaacgatttt
    85261 ttctgacctt caatctattc gctctcagct gcccgaaggc tgggacatca tcagcgatca
    85321 agcaggaatc agtttgcaaa accagcaaaa tttattaaca aatgaccttt gggagatttt
    85381 tttcaaacaa tcagtcagtg tcgaactgct gaaaaatctg ctttttacaa aaaaagttgc
    85441 tgtccctgat tttctagccg accatgggct ttcttatggg acattgaaaa gacatgtgac
    85501 taaaatcaat cagcgtttag caagttacga cttgcagatc gatttgacga aatatacagc
    85561 ttgtattctt ggaaaagagc gtgcgattcg tactttttat catcgattgc tgattccttt
    85621 cactcataac aactattttt tcgaggatta ttcgatccat gaatcccatt attttcaatt
    85681 tttaagaaac ctcagtcaaa ctgaactagc ggttgaaaca gaagagattt tcggaacatg
    85741 ttggttcttt atcaatacga tccgtataaa agcgaattgc cgattagatt cttctatcca
    85801 taccaacagc acactctcct ccctgtatga ctctgcattg aaaaaattgt atttaacgga
    85861 aggtatttat cttaaagaca ctgagctttc ttttgcttct ttctgctttc tggaaagttg
    85921 gaattacaac aataactatg gacaagaaac cgctcgctgc ctgcatcatt ctccattttt
    85981 agaggtcctc gaaacgtttg tagaggaact agcttcagag ctgtctcttg atcaactaaa
    86041 aaaaacatct ctgactgata acttggctct cctcatatta aaatatcatg aatcgccgat
    86101 attgattgaa gcattggacc gtcaatacca tcgtttttta gaaacatatg atcagcaata
    86161 tgcacacttg tatcaacaga aagagaattt actgaacgat ctaaaaaaag aaatagaaat
    86221 caatgaacca aattactttc tccaactctt gtctctactg atccaacaaa cgattttttc
    86281 tatcaaaccg aatttgttca atgtctattt tattttccaa ggagaacctt catggaaggc
    86341 gttcttgcag caggaattaa aagattactt aggaaaacgt gtctgcttct tacctatcga
    86401 actaaccgat ctttccacta tttcttttga aaaaacagat atcttgattt ccaatttccc
    86461 tctagattct ctagatatcc ctattgtata catttcttct attccaacga aaaatgagct
    86521 caatcgtttg actgaactca ctttcaaaac ttttttatag taacggctag cgactgtatt
    86581 ctctagctgt tttttctttt ttgcgaaaca gtttgttcat gatccctttt tctatttcta
    86641 caactaagaa tagaagaatc acatttgctc caatcaataa ctgttgtgtc atcgttaaac
    86701 ttactgtacc gatcagctgc tggccgactg gtagaaaaat aaccatccct tgcaataaag
    86761 caaggatacc taatgaaaga aaaagcgctt tgttttgcaa aagaccttgg ttcaatgacg
    86821 gcttggacaa ctctctgcaa ttgatcatgt aaacagcttg agctaagacg atattttgca
    86881 gaagaatggt ttgctgtaag ctttgccctt caaactgcat agccaataaa taggcaggaa
    86941 ccatgatcag tagggagaca tagatgatcc gaaacaaatg ataaccagat aggattcctt
    87001 gctttggatc tcttggtggg cgcttcatga tttctggatc tggcgactcg aaccctagtg
    87061 cataagataa agtgattgtc gttaccatat tgacccataa aatctgtact ggggacaaag
    87121 gtagcgggcg attcagcata agtgcccaaa tcaagatcaa tccttgagct aatgcagttg
    87181 gcaagaagaa attgatggtt ttttgcaaat tatccaaaat ccgccggccc tcttttacgg
    87241 cttttgcaat cgtatggaaa ttatcatctc ctaaaaccat atcagctgcc tgtttgctta
    87301 cttcactacc tttgattccc atagcaattc ctacatcgga tcttttcaaa gcaggtgcat
    87361 catttacgcc gtctccggtc attccgacga tttctccatt tttctgcaaa gctttaacaa
    87421 ttcgcagttt atgttctggt gtcgttcgag caaagacatc gactattggc acttgctgaa
    87481 tcaactcttc atcagacata agatccaaat cgatcccttc taaaactttg gcagtatgtt
    87541 ttaagccaat ttgttccgca atcgcttttg ctgtgtcttt gtgatcccct gtaatcattt
    87601 tcactgagat ccctgcttgt tggcattcct ttacagcttt gactgcactt tcttttggcg
    87661 gatcgatcaa tccggcgatc cctgcaaaag ttaattgggt taaagtctca tgcgtgagtt
    87721 cctgtttcga atctactttt ttataagcaa atcctaaaac acgctgacct tttctcgcaa
    87781 attctgcagc tgtctgactc catgcttctt gttctgcaga agaaagagtt gatttagcaa
    87841 ataatacttc tggtgcacct ttgacgtaaa tcaccgctcc tttttcctcc gcatgcatcg
    87901 ttgccatata tttgtattca gaactgaacg gaatctttgc ttctattttt ttcaaaggta
    87961 gattggctgt ttctgtgtgt cgaatcagcg caacttctgt cggattgcca cttaaatttt
    88021 ctgttttctg ttcttctttt gtttttaatt cctgacagtt tttcataatg gacaacacgc
    88081 aggcttcttc ttttgcagct acatccacaa ctgtcatttc attttttgtc aatgttcctg
    88141 tcttatctga acagatcact gtcatagaac ctaatgtttc taccgaagga agtcctttga
    88201 tgatcgcctt ttcttttgcc atttcatgta ctccaacgga tagaatcatg gtcaaaacag
    88261 ctggcaatcc ttcgggtacc attgacacca ccaaagcaat cgtagccgaa agaaggatat
    88321 gccaatccat accatatcga aatgtcgtaa aaataactaa gaataggctc aaacaaagaa
    88381 tgccttgaaa aatttgtttg tttaatcggt tgattttttt aatcagaggc gtcgtttgtt
    88441 gttcgaccga ttgcaatgct tgattgattt taccgatttc tgtcgtgttt cctgtttcta
    88501 caacgacacc gactgcagaa ccagattgta caagcgttcc tgaaaaagcc atatttttct
    88561 ggtcgccgat cgattcttct tctgtcaaaa cggcatgact tttttcagat gcttctgatt
    88621 ctcccgtcaa aatcgattct tccactaata aatcatgtgc ttcgattaat cggatatctg
    88681 ctggaacgac atctccggca cgtaagacaa tgacatctcc ttttaccagt tctttcgcag
    88741 ggattttttc ttgcatcccg tttgatagta ctatcgcttc ttgtcccatc agctgcttca
    88801 aaccattcaa tgattcttcc gcttttcttt cttgccagta actaacgagt ccattgacga
    88861 tcactacgaa taaaataata cttccttcaa tatattcact agaagcaaat ttcaaaaaag
    88921 aggcaatgat caacacgatc atcagcagat cagtaaaatg ctttgctatt tttttccatc
    88981 gttttgtctg ctgccttgct tttatttcat tatatccttg atcagtcagg cgtttctgtc
    89041 gttcttcttg cgacaagcct tctgttgatg tttgatagtt tttcaataca tcttctaccg
    89101 tcaatctgta ccagttcatc ctatcccctc ctgtttaatt gatactgtta ttatacgcag
    89161 ggaagttttt tcaggcgtat cgattatttc aggggggtct gaaataattc gttttgcaaa
    89221 atgtatctgg gcctaactga tatttctatt gcttattctt ttatatacaa aatttaagaa
    89281 tacaaaccag ataaccaata aaaaaggaat aaagcgtgtc gtgctttcga ataacaagct
    89341 gaaagcaatt gaagtaaaaa acaccaacaa agcaagatct ttatatcttt gtgtagtgtt
    89401 ccgtttcttt tctttaataa attgaacatg tgcacacaag atcgaagacc acacaaatag
    89461 aaaacaactc gttgtcatgc ctgccaaaag ctgaaaaaac aatcgactat cagctacaaa
    89521 aaaatgtaga caaagaccgg caaaaaataa cattcctgta attaaaatac cgtgtaaagg
    89581 tacgcctttc ttagaaagcg cacaaaacca atggggcaaa cttttttctt ttgctgtttg
    89641 atataaaatc ctgctagtac tgtagactgc actatttgct gaggacaaag aagcaaccaa
    89701 aatcattaga ttgacaattg ctgctccgcc ttggcaatgg atcacttgca gcatagtcac
    89761 taaaggactt tcttttaaat ctaaacaatg ccatggaaca acaaataaaa tcgacaaaat
    89821 cgttcctata tagaaaaaga gaatacgcca aggcagacag ttgattgctt ttttcaaatt
    89881 cttttgcgga tcacttgtct cgccaacggt taagccgatc atttcaattc cagcaaaact
    89941 gaataaaacc ataggaaatg agactaccat tccagcaaag ccaaacggta gtaattcatc
    90001 gatctgtcca aaattttctt tgattccgat agactgggta tgaaaatctg agacactcaa
    90061 tcctataccc aataagataa aaaggaaaat ccccaagatc ttgatgattg agaaaaaaga
    90121 ttccaattca ccaaaccact tgactgcaac aaggttgact gccattaaga tcattagtgc
    90181 gctacctccg cttatcaaag ctggtacatg tacaaaccag aaatggatat acaatcctat
    90241 tgctgttaat tccgcaagtc cagctccaag ccagcaaagc caataagtcc aattgacaaa
    90301 aaatgcccac tttttgccta aataatagtc aacgaaatca ctgatagagt taaatgtact
    90361 atttgctaaa aataaatcac ccatggcccg catcaaacca taaatagcca aaccgcatat
    90421 caaataaacg agaataacag ctggccctgc tgatgcaatt gcctttgctg aacctaaaaa
    90481 taatcctgtt ccaattgcgc cacctaatgc gattaactgg atatttcgat tagttagagt
    90541 ccgtttcatt gccggttttt ccatcaagtt cacctttttc tttttcaagc agcatggcga
    90601 cttcctgttc ccaagaaggt ttctttattt tttcaataca gagtggtact acaaacaaaa
    90661 tgacactgat ggctgtgact gcaattagat tgatccatgt tccagctttc atcgtcagtc
    90721 cgacagaaaa tccgattgcc gcaaataaaa caataacgac agtccaaact ccaagattgc
    90781 tctttttctc tttttttcca attctaaatg gtcgattcaa ctttgcatgt gtataacgca
    90841 atctcaaaac tgcgatcccc atgatgacat agacgataca gtagataaca gtagttgaat
    90901 ttacgagcat caaaaatgct tgattgacgc caggaatcag caaataaaaa agtgcaaaga
    90961 ttgagataat gacagactga gtcaaaataa cggatttgga tacgtcaaat tgattcgtct
    91021 tccaaaaatt gaattttggt ggatacgcac cttttctagc actttctgtc actgtctttg
    91081 ctggtcctga ggcccaagca gataattgga ctgctacacc aatgaagaca agtaaactaa
    91141 acagattcac aataagatgt ggcaaaccaa gaatttggca ataaatcgaa atagattgcg
    91201 cgatattatt caattccatt tgtccttttg gtacgacatt tgctacaact aaagcattca
    91261 aaatattgac gaaaaatata aaaattaacg cggcaaatac tccgcgaata tacgttttaa
    91321 ctggattttc caatcgagtg atatacacag aggacatttc gataccagta aaaataaaca
    91381 tgatcggcgc aaaataaaca aatgaggatg cagtcgtagt atctggtatt aatttgtccc
    91441 atgaaaaact gcctaatgtt ccggttggta cgatcccaac tttgatccac gcagcaaacc
    91501 ctaataagag catcatcgtc aaaggcacgt acaatcctaa ccaaacaccg acttttccgc
    91561 cgatttttgc catatcaaat ttcaaattca aaagagtgat gatccaataa acgactaaaa
    91621 tgacaactaa tgtaaatttg ctgttcaaac caagcggcac attatcgata atattaccaa
    91681 acaacggagc gagtgctgaa gcgaccatca ccatcccagg aaacatttgg acccataaca
    91741 gccatgagac aacgaacccc catttttttc ctagagcatt cgatacccaa agttctggac
    91801 cacctttttg gggaaacatt ccagcaaatt caccagagat caatgagatt ggaaaagcgt
    91861 acaataatac tgcaaccagc atgtaaaaaa tcatcgtcca gctcgttgct gctacatctg
    91921 gaatatttcg tacacttgct acaagggcgc aggtcattcc gataaaggtc atggtcgtga
    91981 tttgctttga tgaaccagta tctttcatct ttattccttc tttcaatagc gacatatgtt
    92041 tttgttttct tttatttttg ttcaagaaca gaaatgacaa ggtttcatcg attcagtaca
    92101 aaacccagta tcaccgaaac atccattgaa cgatcttagt gaactgggtt tgatacatga
    92161 aacgaacgat ccaacttata gaacgtttta taaaaaagaa tatcttggcg aggaatatat
    92221 gaaaaacagg gggaaataca attgttttga tgaagcaaat aattgtctgt tctccatatg
    92281 tctcttgtta ttctttcaca caaaaatcag ttctattgga tgatttcgtt tagtttcttt
    92341 tccattgctt tggcgatttt ttttgtataa aaattgaaaa ttcgatcatc attcaaataa
    92401 ggagtcatga tagccgcacg tagcagagtg attttctgtt cttttttcca ttcttctttc
    92461 gttagaccca ttctttcaac aaaacgaatc ggagaatcac cgtaatcttc ttgtgtaaat
    92521 gtagtatgag aagtaataaa ccgttcacca taaacatcgc catccatata agaagaccga
    92581 tcgaacattt tttcatttaa ttcattgata gcgttcaaat ctgtacagcc ttgttctttg
    92641 aatacccaat tgaccatatt aaaatcagga tgattcaatg gatagacttc gattgttttt
    92701 cctttaacag taaaagtcag atgatcgaga aattctctga atctttgagc tgcctcgatt
    92761 gaagccccaa tcaattgacc atatccggtc acattcaatg gcaatacacg atgtgctgtc
    92821 cagactgcgg cggcggttgc tccagctttt gatccttcca aaatataagc tcctaacata
    92881 tctggagcct ttactgattt ttcaaacaca tatggcgcga aataagaaat gatatttctc
    92941 atatttttat gtttgattac gattcctcca gcagcataag ggacatagcc catcttatga
    93001 ggatctatcg tgacagaatc cgcttctgaa atagctctga acccttcata tacctctttg
    93061 tcaatcgtta cgcagtggtg gaaaacatga tgttcttcaa agaattcagc taaggaagca
    93121 tacggcacaa attctcctgc ttcgtttaga aacagtgaac gcgcatagcc gccatatgcg
    93181 gcatcaacat gtaaatagaa atagatccct tcatctttta acctttctct taattggacg
    93241 attttatcta cactgtctac ttgaccttct tctgtcgttc ctacgacagc aaccacacct
    93301 agaattggga ttttttgtcc agctaattca cgaattgttt tttccaaagc attaatatcc
    93361 attcgataat cctcttgaac tgggatagca accatctgat ctaagccgac tccgcaaata
    93421 tctaacgcct tcatccaaga atagtgtttt gtttgaggga ccagccattt ccctaatctt
    93481 tggatatttt ttccgctacg ggaagaagct gctttgacct catccatttc ttcgtccgta
    93541 aagctttcgg tcatttccaa aatttcttca acagataaat tcaaaagttc ccactcagac
    93601 atttttttca ctttttcagg cagcacttcc ttgacagcta aaggaatcga tttgatgcaa
    93661 cgggcgtacc ataatccttc aagattagca atagaaccat ctgctgcaat gtgtccccat
    93721 ccgtctgtca tattgaataa tgaagcaaaa tcctgtccta cttctgcttc catttgcgaa
    93781 gttgccatag aagattccaa tgcaacatta tttgggttcc acagcatcgc atagttgtaa
    93841 gctaataaag caggcatcaa tgtttcagca ttcatttgtc cccagtaacg gcctgctgaa
    93901 tgccatggga tcgatcctgc gcgtaatcgc tgactgactt catccaagac ctctatcata
    93961 tggtcgcggg tggctagata atctggagtg agcttatctc cttcgctaat tgcaggcata
    94021 tctgaaggaa tataattttc tctccatcca agatgttcat ctaccatctt gttcaacaac
    94081 attttataaa ctggtccatt ttcagctttg tcgcctataa agacggcctt gatatccata
    94141 tctttcattt ttcttccctc catattttgt tggtcaatgt cttgcagata catgtgttta
    94201 tctgtaacac ctcttcacta tagcgccaaa aatgagaaat ttttttttat aacatcaaat
    94261 ccacgtagat ttttacaaac aaaaataaat aaagtcggat ttttctgata attcaacgca
    94321 aaaaagcgaa caatttttca ataaatcagt cttctaaaat agataaccta aaaaaagcga
    94381 a
//
LOCUS       DAYYCY010000002        64067 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000002 DAYYCY010000000
VERSION     DAYYCY010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 64067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64067
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.002"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(27..1523)
                     /gene="lysS"
                     /locus_tag="ACHAZB_000080"
     CDS             complement(27..1523)
                     /gene="lysS"
                     /locus_tag="ACHAZB_000080"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFM8612796.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1634..2638)
                     /gene="dusB"
                     /locus_tag="ACHAZB_000081"
     CDS             complement(1634..2638)
                     /gene="dusB"
                     /locus_tag="ACHAZB_000081"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFM8612797.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2639..3526)
                     /gene="hslO"
                     /locus_tag="ACHAZB_000082"
     CDS             complement(2639..3526)
                     /gene="hslO"
                     /locus_tag="ACHAZB_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFM8612798.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3743..5854)
                     /gene="ftsH"
                     /locus_tag="ACHAZB_000083"
     CDS             complement(3743..5854)
                     /gene="ftsH"
                     /locus_tag="ACHAZB_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFM8612799.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5944..6489)
                     /gene="hpt"
                     /locus_tag="ACHAZB_000084"
     CDS             complement(5944..6489)
                     /gene="hpt"
                     /locus_tag="ACHAZB_000084"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFM8612800.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6489..7934)
                     /gene="tilS"
                     /locus_tag="ACHAZB_000085"
     CDS             complement(6489..7934)
                     /gene="tilS"
                     /locus_tag="ACHAZB_000085"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFM8612801.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8054..8524)
                     /locus_tag="ACHAZB_000086"
     CDS             complement(8054..8524)
                     /locus_tag="ACHAZB_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 domain-containing RNA-binding protein"
                     /protein_id="HFM8612802.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8570..8995)
                     /locus_tag="ACHAZB_000087"
     CDS             complement(8570..8995)
                     /locus_tag="ACHAZB_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HFM8612803.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9062..9331)
                     /locus_tag="ACHAZB_000088"
     CDS             complement(9062..9331)
                     /locus_tag="ACHAZB_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFM8612804.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9342..10937)
                     /locus_tag="ACHAZB_000089"
     CDS             complement(9342..10937)
                     /locus_tag="ACHAZB_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFM8612805.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10952..14473)
                     /gene="mfd"
                     /locus_tag="ACHAZB_000090"
     CDS             complement(10952..14473)
                     /gene="mfd"
                     /locus_tag="ACHAZB_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFM8612806.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14490..15050)
                     /gene="pth"
                     /locus_tag="ACHAZB_000091"
     CDS             complement(14490..15050)
                     /gene="pth"
                     /locus_tag="ACHAZB_000091"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFM8612807.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15403..16377
                     /locus_tag="ACHAZB_000092"
     CDS             15403..16377
                     /locus_tag="ACHAZB_000092"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFM8612808.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16547..17947
                     /locus_tag="ACHAZB_000093"
     CDS             16547..17947
                     /locus_tag="ACHAZB_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HFM8612809.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18025..18747)
                     /locus_tag="ACHAZB_000094"
     CDS             complement(18025..18747)
                     /locus_tag="ACHAZB_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8612810.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18826..19332)
                     /locus_tag="ACHAZB_000095"
     CDS             complement(18826..19332)
                     /locus_tag="ACHAZB_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFM8612811.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19455..21254
                     /locus_tag="ACHAZB_000096"
     CDS             19455..21254
                     /locus_tag="ACHAZB_000096"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HFM8612812.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21322..22896)
                     /locus_tag="ACHAZB_000097"
     CDS             complement(21322..22896)
                     /locus_tag="ACHAZB_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFM8612813.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(23151..23660)
                     /gene="mreD"
                     /locus_tag="ACHAZB_000098"
     CDS             complement(23151..23660)
                     /gene="mreD"
                     /locus_tag="ACHAZB_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFM8612814.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(23664..24518)
                     /gene="mreC"
                     /locus_tag="ACHAZB_000099"
     CDS             complement(23664..24518)
                     /gene="mreC"
                     /locus_tag="ACHAZB_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFM8612815.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(24673..25311)
                     /locus_tag="ACHAZB_000100"
     CDS             complement(24673..25311)
                     /locus_tag="ACHAZB_000100"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HFM8612816.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(25312..25962)
                     /gene="rpe"
                     /locus_tag="ACHAZB_000101"
     CDS             complement(25312..25962)
                     /gene="rpe"
                     /locus_tag="ACHAZB_000101"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFM8612817.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(25975..26874)
                     /gene="rsgA"
                     /locus_tag="ACHAZB_000102"
     CDS             complement(25975..26874)
                     /gene="rsgA"
                     /locus_tag="ACHAZB_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFM8612818.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(27039..29108)
                     /gene="pknB"
                     /locus_tag="ACHAZB_000103"
     CDS             complement(27039..29108)
                     /gene="pknB"
                     /locus_tag="ACHAZB_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HFM8612819.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(29105..29845)
                     /locus_tag="ACHAZB_000104"
     CDS             complement(29105..29845)
                     /locus_tag="ACHAZB_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HFM8612820.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(29858..31216)
                     /gene="rsmB"
                     /locus_tag="ACHAZB_000105"
     CDS             complement(29858..31216)
                     /gene="rsmB"
                     /locus_tag="ACHAZB_000105"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFM8612821.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(31216..32154)
                     /gene="fmt"
                     /locus_tag="ACHAZB_000106"
     CDS             complement(31216..32154)
                     /gene="fmt"
                     /locus_tag="ACHAZB_000106"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFM8612822.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(32147..32638)
                     /gene="def"
                     /locus_tag="ACHAZB_000107"
     CDS             complement(32147..32638)
                     /gene="def"
                     /locus_tag="ACHAZB_000107"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFM8612823.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(32653..35061)
                     /gene="priA"
                     /locus_tag="ACHAZB_000108"
     CDS             complement(32653..35061)
                     /gene="priA"
                     /locus_tag="ACHAZB_000108"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFM8612824.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(35314..35619)
                     /gene="rpoZ"
                     /locus_tag="ACHAZB_000109"
     CDS             complement(35314..35619)
                     /gene="rpoZ"
                     /locus_tag="ACHAZB_000109"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFM8612825.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(35624..36238)
                     /gene="gmk"
                     /locus_tag="ACHAZB_000110"
     CDS             complement(35624..36238)
                     /gene="gmk"
                     /locus_tag="ACHAZB_000110"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFM8612826.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            complement(36725..37009)
                     /locus_tag="ACHAZB_000111"
     CDS             complement(36725..37009)
                     /locus_tag="ACHAZB_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8612827.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(37027..37917)
                     /locus_tag="ACHAZB_000112"
     CDS             complement(37027..37917)
                     /locus_tag="ACHAZB_000112"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFM8612828.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(37937..38155)
                     /locus_tag="ACHAZB_000113"
     CDS             complement(37937..38155)
                     /locus_tag="ACHAZB_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HFM8612829.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(38125..39825)
                     /locus_tag="ACHAZB_000114"
     CDS             complement(38125..39825)
                     /locus_tag="ACHAZB_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFM8612830.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            40092..40865
                     /locus_tag="ACHAZB_000115"
     CDS             40092..40865
                     /locus_tag="ACHAZB_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8612831.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(40946..41515)
                     /gene="thiT"
                     /locus_tag="ACHAZB_000116"
     CDS             complement(40946..41515)
                     /gene="thiT"
                     /locus_tag="ACHAZB_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HFM8612832.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(41581..41683)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(41689..42660)
                     /locus_tag="ACHAZB_000117"
     CDS             complement(41689..42660)
                     /locus_tag="ACHAZB_000117"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFM8612833.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(42754..44127)
                     /gene="glmU"
                     /locus_tag="ACHAZB_000118"
     CDS             complement(42754..44127)
                     /gene="glmU"
                     /locus_tag="ACHAZB_000118"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFM8612834.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(44630..45448)
                     /gene="purR"
                     /locus_tag="ACHAZB_000119"
     CDS             complement(44630..45448)
                     /gene="purR"
                     /locus_tag="ACHAZB_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HFM8612835.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(45615..46439)
                     /locus_tag="ACHAZB_000120"
     CDS             complement(45615..46439)
                     /locus_tag="ACHAZB_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFM8612836.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(46412..47092)
                     /locus_tag="ACHAZB_000121"
     CDS             complement(46412..47092)
                     /locus_tag="ACHAZB_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFM8612837.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(47115..48053)
                     /locus_tag="ACHAZB_000122"
     CDS             complement(47115..48053)
                     /locus_tag="ACHAZB_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFM8612838.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(48298..49149)
                     /gene="ispE"
                     /locus_tag="ACHAZB_000123"
     CDS             complement(48298..49149)
                     /gene="ispE"
                     /locus_tag="ACHAZB_000123"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFM8612839.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(49303..51876)
                     /gene="mprF"
                     /locus_tag="ACHAZB_000124"
     CDS             complement(49303..51876)
                     /gene="mprF"
                     /locus_tag="ACHAZB_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFM8612840.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            52017..52823
                     /gene="proC"
                     /locus_tag="ACHAZB_000125"
     CDS             52017..52823
                     /gene="proC"
                     /locus_tag="ACHAZB_000125"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFM8612841.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(52902..54458)
                     /gene="rny"
                     /locus_tag="ACHAZB_000126"
     CDS             complement(52902..54458)
                     /gene="rny"
                     /locus_tag="ACHAZB_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HFM8612842.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(54869..55918)
                     /gene="recA"
                     /locus_tag="ACHAZB_000127"
     CDS             complement(54869..55918)
                     /gene="recA"
                     /locus_tag="ACHAZB_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFM8612843.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(56027..57268)
                     /locus_tag="ACHAZB_000128"
     CDS             complement(56027..57268)
                     /locus_tag="ACHAZB_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HFM8612844.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(57508..58086)
                     /gene="pgsA"
                     /locus_tag="ACHAZB_000129"
     CDS             complement(57508..58086)
                     /gene="pgsA"
                     /locus_tag="ACHAZB_000129"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFM8612845.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(58295..59218)
                     /locus_tag="ACHAZB_000130"
     CDS             complement(58295..59218)
                     /locus_tag="ACHAZB_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HFM8612846.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(59957..61243)
                     /locus_tag="ACHAZB_000131"
     CDS             complement(59957..61243)
                     /locus_tag="ACHAZB_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFM8612847.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(61236..62504)
                     /locus_tag="ACHAZB_000132"
     CDS             complement(61236..62504)
                     /locus_tag="ACHAZB_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFM8612848.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            62756..63217
                     /gene="mscL"
                     /locus_tag="ACHAZB_000133"
     CDS             62756..63217
                     /gene="mscL"
                     /locus_tag="ACHAZB_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFM8612849.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(63277..63723)
                     /locus_tag="ACHAZB_000134"
     CDS             complement(63277..63723)
                     /locus_tag="ACHAZB_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8612850.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(63816..64061)
                     /locus_tag="ACHAZB_000135"
     CDS             complement(63816..64061)
                     /locus_tag="ACHAZB_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HFM8612851.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
BASE COUNT        17703 a        13918 c        11477 g        20969 t
ORIGIN      
        1 gaattcctta gacagctttt attcgattat ctcattgttg ggaatagcaa aacatcccga
       61 attgattgtg catcagttag caacattact aatctgtcga tcccgatgcc tagaccacca
      121 gttggcggca taccgtattc taaggcttcc aagaagtctt cgtctacacc gtgagcttcg
      181 tcatttccta gttcacgttc tttttcttgc gcttcgaatc tttctctttg atcaattgga
      241 tcgttcagtt ctgtaaaggc attagcaaat tcgcgcccca ctatgaatag ttcgaatcga
      301 tctgtgaatc gaggatcttc tggatttttc ttcgctaaag gcgaaacttc gactggatgt
      361 ccgtatacaa acgtcggttg tgtcaatgtt tcttcaacaa atgtttcaaa gaattcattg
      421 ataacatgtc cgacacccat agcttcggtt acttctacat tgtgtttttc ggctaaagct
      481 aatgcttctt ccaatgtcat ttcttgccag aagtctacac cagtttgttc tttgattgca
      541 tctaccatat ggatgcgttt aaagtcactt tctaagtcga cagcttgtcc atcatatgtg
      601 atttttgctg ttcctagtac tttttcagct gcgttacgga taataccttc tgtcagattc
      661 attacatcat tgaaatcagt atatgctgtg tatgcttcta acatcgtaaa ttctggatta
      721 tgtgtagtat caattccttc attacggaaa acgcggccga tttcataaac tttttccatt
      781 ccgccaacaa ttagtcgttt taaatgaagt tctagcgcaa tacgcaaata taaatcaatg
      841 tccagtgcat tatgatgcgt gataaatggg cgtgcggcag ctccacctgc ctcattgtgc
      901 aatactggtg tttctacttc aatatagcca ttgccatcta aatagcgacg gatttcgctg
      961 ataatttggc ttcgtttcat aaatcggtca aagctttcgc gattactgat taaatccaag
     1021 tagcgttgtc tgtagcgttg ttcaatattc gttaagccat ggtatttatc tggcagtgga
     1081 cgaagtgctt ttgacaaaat cgtgatttct gaagctttga tagaaacttc gcctgtgtct
     1141 gttttcatga tttgtccagt tacaccaaag aaatctccaa ggtctgcatg tttgaatact
     1201 tcataggcct catcacctac ttggtctttt cttacataaa tttgaatttg gccttcacga
     1261 tcttgtagat gcgcaaatcc agctttccct tttcctcggc gagtcatcat acgaccagct
     1321 acactagcgg tcaatcccat ttctgctagt tcttcttttg ttcttggatc aaatagttca
     1381 tgcaattctt ctgagttgtg cgtgcgttca aatcgttttc caaatggatc gattccatct
     1441 tcacgcaatt gttccatttt ttgacgacgt acaagcattt gatcatttaa atcttctact
     1501 tgatgttgtt gttcctgtgt cacaaaagtt cctccattct catgttaatc tctttcctat
     1561 aatgccaatg aaacacaaaa aaagcaagcc gaacttggaa tttcggcttg aaaattcacg
     1621 aaaaacttag aaatcacaac gtttgtttca atagtacttt ttccgctttt tctacaaatt
     1681 gatccagcag gtcattgatt tcttgctgtt tttctgtctg attgattgct actttcactt
     1741 tcgctgctcg aggaatccct tttaaataat aagccgcatg tgttctgaat tctctacaag
     1801 ctactttttc tcctttgaga tctgctaagc gttgtaaatg aagtttagct gtagcaattt
     1861 tttcggctgg tgttggttca ggaatcagtt cccctgtttc gagataatgc tgtgtgcggt
     1921 gaatcatcca tggattgccc agcgctgcac gaccaatcat cacaccgtct gcaccgacat
     1981 agtcaagcat ccattttgca tcttctggcg ttttgacatc cccgttcccc ataaatggaa
     2041 tcgtcaaatg ttttttcact tctttcaata cttcccagtc cgcttttcct tcatacatct
     2101 gaacccgtgt acgtccatgc atagcgatgg cacttgctcc agctgattgt gctgctaatg
     2161 cattttctac agcaaagacg tgctcttcat cccaaccaat acgcatcttg attgttactg
     2221 ggatatttac tgctgaagaa acagcttgga ccatttcgta tactttctca ggatctaaaa
     2281 gccattttgc tcctgcttct gctttgatta ctttatttac tggacagccc atattgatat
     2341 ctattatatc ggcagctgtg ttttcttcga caaattgagc agcttcaact agactatctt
     2401 tatttccgcc aaatatctgt aaacttaatg gatgttctgt ttcttcaatg tggagcatct
     2461 ctaaagtttt tttgtttctg aaatgaatac cttggtcact gatcatttca catacgacta
     2521 agcccgcacc aaattctttg acagtgactc gaaatgctga atttgttatt ccagccatcg
     2581 gtgcaacaac gacacgatta gggatttcca cattgccaat tttccatgtg tgttccactt
     2641 aaatccctcc taaggcttct ttcttcaatc catctaattc ttcttcgcta tacttgtatt
     2701 tgttattaca gaaagaacaa accgcttctg caccgtgatc ttcgtctatc attgcttgga
     2761 tttcttctgc acctaacgcg ataattgctt ctgcaaattt ttcttttgaa cagtcacatt
     2821 tgaattgaac aggcattttt tccaaaatgt tcaattcttc tgtaccaagc aatcctttca
     2881 atatttcttc tggtgtctgt ccttcatcta agagggtaga gattcttggt gtcgttttta
     2941 attgttcttc gatcttgctg attgtttctt catcggcacc tggcatgatt tggatcatga
     3001 atcctcccgc cgtcttgatg ctgtcgtcag tattgaccaa tacactcagt ccaacagctg
     3061 aagggatttg ttctgagata gcaagatagt aagtaaaatc ttcagcgatt tctccagaaa
     3121 caatcggcgt ttgcccagaa aaaggttctt ttaatcctaa gtcttttgtg acagtaaaca
     3181 ttccttgtgt tccaactgct ccccgcacat cgattttacc ttcactattt agaggaagac
     3241 tgagatgcgg atttttgatg taacctttca catctccctt tccatttgca tctactacaa
     3301 ttcctgttgc tggaccatcg ccttggattc gaacagttag tttgtcttct cctttgagcg
     3361 tcgcaccaag tagcagacta ccgaccaatg tccgtcccaa agcagctgag gaggtgtgcc
     3421 aagtatcatg acgtctttgt gcctccgcta cggtatttgt tgcttgaacg gcgtatgctc
     3481 ggacgaatcc gtcgtgagca agtgccttaa ccaaataatc tttcatttgt tatgtactcc
     3541 tttttccgtt tttaaactca tagtgaatca tactagcaac tactgctttt ttcaactttg
     3601 gaatgcttct tttaaaaata attcagaatc atctagaaaa caggacatca ccctatttac
     3661 aacttaaata aagaaatctc ccgaaaaccg gtttttgttc cggttttcgg gagtcatatc
     3721 agatcaattg acctatattt ttttatttgt attgatcgtc tgaatgatta tcttctgatt
     3781 gttgagaatc tgaatcatgt tctgctttat tttcctcatt cagttcatgt tttgcttctt
     3841 caaaatcacg tttttcttcc gcttgttttt cagcgtcttt ttcttccaaa gcacgttttg
     3901 cttcttcaaa agttttcgct tctttttcgc ttggataatc atttgactca gtaccttcag
     3961 gcatcacacc gtgttcaaac aatgacttga ttgctttggc atccaatgtt tcgaattcca
     4021 aaagtttttc agcaatcagt ttatgctgtg cacgatgagc ttcgatgatt tctcgagctt
     4081 tatcatgagc ttccatcaag atttttcgta cttcttgatc aatctcgaaa gcgacttgtt
     4141 ccgagtaagc ttttgtttga ccatagtcgc ggccaacaaa cacttgatgg tttccttcat
     4201 attgaacagg acctaaacga tcactcattc catattctgt taccatgctt cgtgctagag
     4261 ctgtcgcttg ctcaaagtca ttggaagcac cagttgattg gacattgaag atgatttctt
     4321 cagctgtacg tccaccaagt aagcctacga tttgttcaaa catatcttct ttcgtcatca
     4381 agaactgatc ttcttttggt aaagcgatca tatatccgcc ggcgcgtccg cgagggatga
     4441 tcgttacttt gtggacgacc cgagcgcgac tcaatactaa tccgacaatc gtatgaccag
     4501 cttcgtgata agcaaccatt tcgcgttctt ttttgctgat tacgcgatct tttttagctg
     4561 gacctgcaat cacgcgatct tccgcttcat cgatatcaga agcatcgatt ttctttttat
     4621 ttcttctagc agctactaac gctgcttcat tcaagacatt ttctaaatct gctccggcaa
     4681 aacctggtgt ctgttgagcc acaactttca agtcaacgtc atctgccatc ggtttattcc
     4741 gtgcatggac tcgcaagatt gcttcacgac cttttacatc tggacgtcca actaaaatct
     4801 ggcggtcaaa acggcctgga cgtagtaacg ctgggtctag tacatcagaa cggttggttg
     4861 cagcaatgac gattactcct tcatttccgt cgaagccatc catttctaca agcaattggt
     4921 tcaatgtttg ttcgcgttca tcatgtccgc cgcccatacc ggcaccacgt tgacgaccta
     4981 ccgcatcaat ttcatcaatg aaaatgatag ctggtgcgtt tttcttcgct gtttcaaaca
     5041 aatcacgaac acgacttgct ccaacaccga caaacatttc tacgaaatca gaacccgaga
     5101 tcgagtaaaa cggtactcca gcttctccgg caacagcttt tgctaataag gttttacctg
     5161 ttcctggagg tccttctaaa agtacacctg ccgggatacg ggcacctaat tcaacaaatc
     5221 ggcgaggatc tttcagaaat tcaacaactt caactagttc ttgtttttct tcttccgcac
     5281 ccgctacatc agaaaaacgt acgcgattag cttttttgtc tgcttctttg gcttttgatt
     5341 taccaaagtt catgacacgg ccgccaccgc caccgccgcc gccttgttgt cccatcatca
     5401 tgtaaaagaa aaagatgatg attacgattg gcaagaagct aagcaatagg gaaacccata
     5461 taccgctgct tgattcttct tttactgttg tagcaacttt gttttctttt gccagttctg
     5521 ttacttggct caatgttgta tcatttggca aaatgattgt tgtgaaatgg gtagtagtcg
     5581 tatctgttga acctaagata gctaacccac cgctgttagc gacggtctgc tgttctttgt
     5641 attcaccagt gatcttatat acaccatttg caggctgcac ttcaaattct ttgatcttgc
     5701 ctgcttgtag ttgttctgtg aattttgagt actcgatatc tgacgatcgc tggccgttat
     5761 taccaaaaat gaaataaacg accattacca tcgctaagat tacgaggaca taatacaagg
     5821 catttttcat catgccgttg ttctttttgt tcatagttgc cctccctaag cgtcaattta
     5881 atttaacagt tttttatcaa tcctgaccat tctattgtac cataatttaa ttgagataca
     5941 gcattaattt gattgataaa cactaggttt caatacaccg atgtacggaa ggttacgata
     6001 tgcttctgca taatccaagc cataaccgac cacaaattca tttggtacgt tgaagccaac
     6061 ataatctgct tcgatattta ctactcttcc ttcaggtttg tcaagtagtg taacaatttt
     6121 gactgatttt gctttgcggt atttgaaaag atccactaaa taagcaagtg ttcttccgct
     6181 gtcaatgata tcttcaacga tcaacagatc gcgaccttct acattcgtat ctaagtcttt
     6241 gacgattttt acttcccctg acgagactgt tgcattcccg tagcttgaaa catccatgaa
     6301 atccaattcc aaatacgtat cgatagaacg aacgatatct gccatgaatg gtaccgctcc
     6361 ttttaagaca cctactacta ggggattggt gttttggtaa atttctgtta actctttgcc
     6421 tagttctttg caacgaactt gtatctcttc ttgtgagatc aaaattctct caatatcctt
     6481 ttccaacatc aggttctcct tccaattgct tctttttgat atttgtaaag aagtatgtag
     6541 tgtattttat cagtttcttc acgaatactc aaatatgaaa aggcaaaagg tatcaagcta
     6601 tatatatttc ttttggcatc tgtaatgatc caagcatgct gcctttgtga attaggtact
     6661 ttctgatcga taaaatatct agatactttt tttgtgagat tctctgttaa tttaatgcgg
     6721 tctcctgctt ttcttttttc gacatacagt tgcacatcag caggaatcca aatgtcatga
     6781 gaaaatagag accatgaatc tgttgctttg atgtcccctg cattttcagg tttgaccaga
     6841 ccaatccatt ctgtttcatt caaatatatt ccttgttctg gaatcagcaa caagcgctgg
     6901 tcttctgtct ggctagttgc ttgtttttct tttttagaga gtacaacaaa atgatactct
     6961 cttttcagta tccaattatt gccaatactg agttgccatt ggcttttttt ctgttgccac
     7021 tggtctaaaa tcgactccat ttgttgttct ttcaaaataa ttcctgtttt tgagaatgct
     7081 ttgtcgaaaa aggagtaaaa aaaatagtat cgttcagcat ctgtcatttc ttcaatcatt
     7141 tctgcagaaa atcgaaattg ttcctcccac tgttcgactt ccttttcgat caactgtccc
     7201 atatattttt gaatgacttg gtctgcccat tggatctgtt gcgaaaattg ctggaaatgt
     7261 ctcaacgcct gtgtattttc ttgtttcaat tgaggaacta ccaaatgacg aagccgattt
     7321 ctctggatat ctaacaattg gtttgtctga tcctcaaagt aaacaagctg cgattccgct
     7381 gcataagtat ataggtcttc tttgctaaaa ctcagcaacg gacgaatcag tctgcccgta
     7441 gcaaatggtt gtacttcttt gattccagaa aaagtattta actgtccccc acgaacgatt
     7501 ttcattaaca ccgtctcgat ttgatcatct ccgtgatgag cagtcatcaa cgtgtcatat
     7561 tgtccttctt ccatcacgcg agtgaattct tgataacgaa agttacgagc ttctgtttct
     7621 atggcggatt tggctggatc ttcccaaata cttacatata aagggagatt cttctcttca
     7681 caatatgagc gaagataggc agcttcttct gctgattctt ctcttaattg gtgattgatg
     7741 tggacgatac ctaaagaaaa accgatttgg cgttgcactt gtaaaagtaa atggagcaaa
     7801 accatcgaat caacaccacc tgataccgct gctaaaattc tcgatccttg ttgccagaat
     7861 ccttgttcat tcccgtgccg aataaatgac tgctgtaata gcggtgcttt tttcccgcct
     7921 ttttcaacgt tcatcctttc cacacccttt cttcctaaaa aaaagagagc gggaaaacgt
     7981 ctaaaacgac aattaacctg ccctctgttg atactatgac ccaaatagtc gcactatcat
     8041 tatttgcggc tttttagctg cgacgtccgc cacggccacc gcgctttcct tctgtgtttc
     8101 gcttcaatga agataagcgg tcatcgctat ctttcaaaaa tgaactcatc aatgaatcga
     8161 agtcttcttt ttgagctgaa ggttgactgc gagaaaaaga ttttttctgt gctggacgat
     8221 ttcggttgtc acggggatct ttttgttgat attttttagg acggctttct tctccagatt
     8281 cagcaggttg ttcctgcgct ttccggatag atagtccgat ttttccatca tcatttactg
     8341 aaataacttt aactgttact tcatctccta cacttaaaac atcatggatg tcttttacat
     8401 agccatttga cacttcgcta atatgaacta gacccgtttt tccagcacct agatcaataa
     8461 acgcaccaaa atttgtgatt cctgatactt ttccttgcag ttttgctcct acctcgattg
     8521 acataaaaaa aatgttcctc ctaattattt ccctttttta tattcctgct tattttgcag
     8581 ttgtagaact agttgattga tctgtttcat ttgtcgagct gtctgtattt gattctaata
     8641 taggataaac tttttcaccg tcttttgagt aaaagaattt gcttctggca agtttagcta
     8701 catactcatc atctttcagc aatgaaacat cttggtgcag ctgatcaacg tctgcgttga
     8761 cttctttcat ttcagtattt gcttcgactc tcaattcgtt caattgatgc atcctttgca
     8821 tgtcatggaa aatctgcaca cctattatcg cgaaaatgac caaagcacca acaaaaattg
     8881 cagccagtct gcgtcttcga aaaatcagtt gacgttgttg ttgctgaaat tcagcatact
     8941 ttttctttgc atattccgta tctagcgcag cgattttatt gctttttttc gtcacttgga
     9001 ttcgctccaa tctttttgat tgtctttttc attataccaa tatcgtgaaa gcttgtctaa
     9061 tctatttctc tagtgtttct gtgcgatttt cagaaataat ttcgtacatt ttttgtgcat
     9121 cttctttttt ggttgattca tgtaattccg taattttcac ttccaacgtt ttgttgccaa
     9181 actggacttt caaaatatcg cctactttga catcactgga agatttcgcc aatttaccgt
     9241 tgatttgaat tcttccttta tcagctactt cttttgctac tgatcgtctt ttgatgattc
     9301 gggaaatttt taaaaattta tctaatcgca tatcttattc ctcacttttt aattttttta
     9361 tttaatcgca ggattttctt accaaaaggc aacattagcc attctcgtac tgtaaacaga
     9421 cgaagacgaa tcactgcata aatgaacaca gaagccccga tcccaactcc tatcacgctg
     9481 acaaaaaaag cagtggaacg atgagttaca ggaccaaata ctatattcaa tacaccataa
     9541 tacagcagca gtactactgt catacaaaga aggctgaaca tcaacttccg tccaaaatgc
     9601 cgttctttcc aaaaagcatt tagctctttt gtttcaattc gaacaaaata ccaaagcgtc
     9661 acacctaatc ctaaaagggt agacaggctt gcacctgctg ttcctaataa accggtcaga
     9721 aaagaagtag tcaatccttt taccaatagt ccccatcctg cgccttttaa agaagggcga
     9781 aacgagttct tgctttgagc aatactttga taagcttgga tgactgcagt gaaagcaatg
     9841 gaaaaaacaa ataatacaag cgctgcattt cccgcataat ctttaaacaa cgcataattg
     9901 atataaggga gcagcaaggc taatcccaaa gacgcagcta atgctaaagc ggtcgtcaat
     9961 ctaagataga ttttggctgt ctgtaaaaac tgacgataga tccggttggt caaatgacga
    10021 gtcaacgcag gcaggaatgt cgcacttaat gcagtggcaa tcactaaacc taattgtact
    10081 aaaggctgac ctcgatcata gacccctttt gctatcttcg caccgtattc tgacagtccg
    10141 taaacttcca aagcattttt tacaaagaaa gagtcgatca gctggaaaaa gataagcaat
    10201 ccactataaa tcgaaaccag tccgccttct accagaaaac gccggatcaa gggctttgac
    10261 ggctgtttga acaatggaaa ccgtcggaag cttaacgctc ctccatgaat cttttgttca
    10321 tagtagtgaa ggatcccata tgcgcataaa ccgccaaaaa cagctccact cattgctgcc
    10381 gtccctgttt ggtacaccgt ccatccaagc tttctaaaag caaacgcact acagacaatc
    10441 acaaaaacac gaacgaactg ctcaactact tgagaaacag cagttggttc catcaagaat
    10501 cttccttgga aattccctcg ataaaaagat agaccaggca tcagcaaaaa ggtaaaggaa
    10561 actacttgga tcagcggttt caattgtatg tctcccatca tccaagcaat ccagctgcta
    10621 aatgcaaaga caaacaacca caaaaagaca cctgtccaaa atactaaggg ataaagattt
    10681 tgcaaagctt gctttcgctc gaaaggattc tttctttctg caacgtactt tgatatgaac
    10741 tgcggaagac cggataaagc taaagtcatt gctatcccat aaattggata aacttgctga
    10801 taaacataaa aaccttcgtc gccgactaag ttttggaggg gaattcggta aagggcactt
    10861 aatacttttg ctatgaaaga agcaatcgtc aatacaaatg ctccttgcat gacgcgccgc
    10921 atttctttat gatccaaatc attgctcctt cctacgctga cagatatttt tgctcacgca
    10981 acgctttgac aaacttttgg atttcctgaa gccatactgc ttcttccatt tggttgggga
    11041 tcgtcaagcg tatcaccatc tgttcttttt caacgcctag actggctttt agttttgtcg
    11101 ctgtcagtgc ttcgaataac tgttccacac tatagcgttt tgttccaact ttacttagag
    11161 taaagactat atgcgcctcc tgtttcctta tcgtttctag gagcgctcga tcgccatcca
    11221 ttttgatctg cccaattgtc aagagatgtg ctacttcttc tggatattct ccaaaacggt
    11281 cgagcagatc atcttctagt tcatctagca tttcttgcga atcaagttca cgaattcgtt
    11341 tatagatctc gattttctgc cgttgatctg ccacgtatgt ttcaggtaga tacgcatcga
    11401 caccgaggtc gatctcaaca gtggtcttct ccacttgact attctttcct tgtttacgag
    11461 aaacagcttc gcttagcatt tgagaataca tatcgaaacc gactgcatca ataaatccat
    11521 gctgctgagc acctaagaga ttacctgcac cgcgaatgga aagatcccgc atcgcaatct
    11581 tgaatccaga tcccagttct gtaaaatctt tgattgcctg aagacgtttt tcactgactt
    11641 cgttaaggat cttctgctgc tcatacataa agtaagcata tgcgacacgg ctgcttcttc
    11701 ctacccgccc tcgcaattgg tataacgtgg ataatcccat ataatcagcg ttctcaacga
    11761 acaacgtatt tgcgttcggg atatctactc ctgtttcaat gatggttgta gtgacaagaa
    11821 catcatattg accttcaata aattcaaaaa gcgtattttc taattgcgcc tctgtcattt
    11881 gcccatgagc ataaccgatc cgtgcttccg gaacgagttc ttggatttct tctacttttt
    11941 gttcgatcgt ttctacccga ttatataaat agaatacttg tccacctcgt cccatttctc
    12001 gatggattgc ttcgcgaata gccccaggat ttttttccat aacatacgtt tggatcggat
    12061 aacgattcgc aggcggtgtt tcgattactg aaagatcccg cacgcctaac atcgacatat
    12121 gcaaagttct cggaatcggt gttgcagtca gagtcaaaac atccacttgt gcacgtaact
    12181 gtttcaatcg ttccttatgt tttacaccaa aacgttgttc ttcgtcaatg atcagtagac
    12241 ccaaatcgct aaattcaata tcttttgaca aaattcggtg ggttcctaca acaatatcaa
    12301 cttgacctgt acgcagttgt tcgatggttt ctttttgttg ctttcttgtt ctgaaacgac
    12361 ttaatagccc gatatttacg gggaaacctt caaatctttc cagcatcgtt tcatagtgct
    12421 gctgtgccaa gatcgttgtt ggaactaaaa aggctacttg tttgctttcc tttatcgctt
    12481 taaaggccgc tcgaagcgct acttctgttt tcccataacc aacatcacct acgagtagtc
    12541 gatccattgg tttttctttt tccatatccc gtttgatctc cgctgcactt ctcaactggt
    12601 catcagtttc agagtagggg aaggcatttt cgaattcttt ttggtaggcg tcatcaggtc
    12661 caaaagcata tcctttttct gactctcttg cggcatataa tttgattaga tcgtcagcga
    12721 tatcctcgat ttttgaagag acttttcgct tggttttcgt ccattcactt ccgcctagct
    12781 tattgatgcg aggcgctttg gcttcagagg cgacatattt ttggatcaga ttcagttgag
    12841 taacaggaat aaacagtttg tcgtcgtttt gatacaaaat agtcatatag tcttggtgaa
    12901 cgccatctac ttctagcgtt tccatcccaa tgtatttccc gataccatga ttggcatgga
    12961 caacgtaatc tcctgctttt aactcattat agcttttcaa tcgctctgcg tttgttactg
    13021 tttgacgtct cgtttgtttt ttcgtcgttt tttggaaaat ttctttttct gtgatagcta
    13081 ctattttttc ttgaggaagt tcaaaaccag cctgcaaagc tccttcaact aattgtacct
    13141 gtcctctgat caatgcattt ttttctgttt ctactactaa tatgtcttca tctcgaagca
    13201 tttgttctgc tttgcgtatt ctgtcctttg tcggaataaa gatcacaaca gtctgttttt
    13261 gcttgcgcca acggtccatt tctgttttca gcaatggcat ttgtccgaag aactgttgca
    13321 tcgtccgata ttgaaaatta tggattgctt ggaaacgaag atttcccatc cctttttgga
    13381 agagtgagaa aaaggttgtg gtgaactttt ccttctgcac gattttttgt atatctgctg
    13441 aaaatgtctg ctcagaaaac acccgcaatt cgctgatttt ttgagtctgc cattctgccg
    13501 cttccctttc gatttcccga tttgtttcca tgattcgctg ataatcatca acgactagta
    13561 aactatttga agggaaataa tccaagatcg tcacagattc ttggtaaaga agatctgagt
    13621 agaattttgc ttcttctgtc gggacacctg cttgccagct tgaagtcagc tgaccgaagt
    13681 aatcttgcaa aaactcgcgg tctgcttctt ctgtggtcgc agcaagccgt ttttctagca
    13741 ttttttgcat ttgctctatt ccatgttcta gatcctctgg tccgtagact tgttcactcg
    13801 ttggggaaac ccagatttct tccgtttttt cgattgagcg ttgtgtttct gcttcaaaat
    13861 accgcataga atctatctct acatcaaata gttctacacg aacaggatag gttgtcgtta
    13921 acgggtaaat atcgataatg cttcctcgaa tactgaattc tcccggtttt gcgaccatct
    13981 cttctcgtac atagcccatc aaagtcaatt ggcgaggaag attttccacg tctatctcat
    14041 caccaatatg ccaattcaat tgcgcctgta gccaagtttt cttattcggc agtcgtttgt
    14101 gcaaagatgc gactggcaac aaatagatac ctgctttttt ttcttgaagt gcgttaagcg
    14161 ttgcaactct ctcgcctcgc gcttctggag aagaaaaggc catttcagca gaaagcactt
    14221 catccactgg aaataaatgg atctgatctt gcggaatcac atgttgcaga tcttctgcta
    14281 actgattcgc ataatacaaa tttggcatca gaataatcat gtttttattt gttttcttaa
    14341 acgctcgagc aaatagaagt gttttcgctg agcccgctaa tcctgtgatt agttgtctag
    14401 gcattttttc ttctaaattg ctgacccatt cttttacttg cggatctttt ccgactaatt
    14461 gaataatatc cattcctttt cctccttcct caattgaatc gattcatcgc atccacgaac
    14521 gaatgccctt ctatgagata ttcgactgct ttaacagatt gacacatgct ttcttctatc
    14581 aaaggctgat tttctttgct gaaaggacta agcacatgct ggactactgt ttttttccct
    14641 tctggcctgc cgattcccac cttgattcga tcaaagacat tcgtattcaa atgggaaata
    14701 atacttttga taccgttatg cccgccggca cttccctttt gacggagccg gatttttccc
    14761 actgccagat ccaaatcatc gtagattacg actaattcct ctggataaat cccaaaataa
    14821 gtcattagtg ggccgactgc acgaccagat tcattcatga atgtttgtgg tttgactaaa
    14881 aggatctttt ctccattatg aaaaaattcg gcaacttctg cttcgaacgg attttttttg
    14941 aaagtagctt gatgctcgcg tgcaatttta tccacgacca taaagccgat attgtgtttt
    15001 gtatattggt atttggtacc tgggtttcct aacccgacga tcattttcat ttttgttttc
    15061 cacctaattc taaaaagcag cacaaaaggc tggactgcta attagcggtc cagtccatcg
    15121 agcttttttc ttttttacac ttgtcaatgc atagtataac acaaaacaag gcgtttcggc
    15181 taaaagagaa tcgttaataa atgataaaga aattaaaaat ttcttcaaag tttaatgaaa
    15241 taattgatat ataaaaatag tgaaataacg cacatgtaag cttttacata taatacaacc
    15301 ttgttatctg ttatatttta tagccaaata gcgtgacatg gggcttgaga aatgttagca
    15361 taagcatggg aaaaaacaaa tcaatggaag gattgatact aaatgactgc agctgcagaa
    15421 aaaaaagatc atcaaaaagt tattcttgtt ggagacggcg ccgtaggttc tagctatgcc
    15481 tttgctctag ttactcaaaa tatcgctcaa gaagtaggta ttgtggatat caatactgct
    15541 aaaactgaag gagacgcaat tgacttgtct cacgctttgg catttacttc acctaaaaaa
    15601 atctattctg ctacttacgc agacgcacat gatgctgact tagttgttat tacagctggc
    15661 gcaccacaaa aaccaggtga aactcgttta gacttagtaa acaaaaacct aaaaatcaac
    15721 cgcgatgttg tcacacaaat cgttgaatca ggatttaacg gtatcttttt agttgccgct
    15781 aacccagtag atattttgac ttactcaact tggaaattct caggtttccc taaagaacgc
    15841 gtaatcggtt caggtacctc tcttgactca gctcgtttcc gtcaagcact tgcggaatta
    15901 gttgacgttg atgcacgtaa cgtccatgct tacatcttag gtgaacacgg agattcagaa
    15961 ttcccagttt ggtcacatgc aaatgttgcc ggcttgcaaa tctatgaatg ggtaaaaaac
    16021 aacccagaaa tcgatgaaga agctatggtt aacctattct ttggcgtacg tgacgctgct
    16081 tacacaatta tcgagaaaaa aggcgctact ttctatggta tcgcagtagc attagcacgt
    16141 atcactcgtg ccatcttaga tgatgaaaat gctgttcttc ctttatcagt attcatgaat
    16201 ggcgaatatg gattgaacga catctacatt ggtgcacctg ctgttatcaa ccgtcaaggt
    16261 atccaaaaag taatcgaaat tcctttaagt gacagcgagc aagaccgtat ggctgcttct
    16321 gctaagcaat taaaagaaat tctagatgaa gcttttgcta aactagacgc tgaataatct
    16381 ttgaacatga cggaaaacat ctattcggtg ttttccgttt ttttatttta tcacccttat
    16441 ggtttctaaa ttttttcata aagtagagat aaccttcgtc ttttcctcta aattatgcta
    16501 tcatgagacc gttccaccct aaacttttac ttaaggagtt gtcgatatga caagatcttc
    16561 gcatcgaaaa aaatcgaaac atgttgcttt gctttcagtt cttggtgttc tcttagtgct
    16621 gatcagcttt tatacctatc gcagtacata ctacacaaag cattttttgc cgaatacaga
    16681 aataaatgga ataaatgtca gcaatctaac agtcgaaaaa gcaaatgaaa aattaaaaga
    16741 agcttattct gataaaaaac tggctattaa agaagatgga aaagtctggc aggaagtacc
    16801 aaaatccgag ctcggctaca aagatgattt cacttcggag ctttctccga tcctaaatga
    16861 acaaaacgga tggacttggg gaatgactta tgtctcagcg gcagaaaaac aagaaataga
    16921 tcctgtcagt caaaaccacc aaaaactaga cactaccgtt cagacattga caactaagct
    16981 gactgatttg aacaaagacc ggacacctac tactgatgca acgatcgaaa aagcaggtga
    17041 ctctttcgtt atcaagcctg aagtcaatgg aaatacgatc gacgtcgatg ctgcggtcaa
    17101 acaattaaaa agtgcggtaa actctggtaa agatacaatt gaattgactg aattcaaaga
    17161 aaaaccaaaa gtaacatctg aagacagctc actgaaagaa caactcgcat ccatgaatgc
    17221 tatcgcaaat gtaaaagcta cttacagtat aaacggtgag actttccaga tcccatcaag
    17281 tgatattatg agttggctga cctacaatga tggaaaagtc gatttagata cagaacaagt
    17341 acgtcaatat gtaacagatt taggcacaaa gtataacaca agtacgaatg acacaaaatt
    17401 taaaagtaca aaaagaggag aagttacggt cccagttggc acttacagtt ggacgatcca
    17461 aacagacagt gagacagaag cattgaaaaa agcgattctg gcaggccaag attttactcg
    17521 ttcacccatt gtgcaaggtg gcacaaccgc tgatcatcca ttgatcgaag atacgtatat
    17581 cgaagttgat ctggagaacc agcatatgtg gtattacaaa gacggaaaag tggcccttga
    17641 aacagatatc gtttccggaa agccaactac acctacacct gctggagtct tctatgtttg
    17701 gaataaagaa gaagatgcaa ctttaaaagg aacaaatgac gatggaacac cttacgagag
    17761 tccggtaaac tactggatgc cgattgactg gacaggtgtt ggtatccacg attcagactg
    17821 gcaacctgaa tacggtggag acttatggaa aacccgcggt tcacacggct gcatcaacac
    17881 cccaccaagt gtaatgaagg aattattcgg gatggtcgaa aaaggaacgc ctgtattggt
    17941 cttctaacaa aaataatata taaaaagaaa gcacccgtac gattggtaca ggtgctttct
    18001 ttttattatc taacaatgtg ctacttacat ttcagcagca gtaaagactt ctgaaacatc
    18061 atcatcgtct tctagcttat ctactagacg ttccaattgc tctttttgtt cctcatttaa
    18121 atcaatcgtc gtttgtggaa tcatcgtcaa ttctgcttgc gccaaagtga actctttttc
    18181 caactcatct cgtacagcag caaaatcttc tggtgcagta taaatctcga atacttcagg
    18241 agaagtttgc agatcttctg cccccgcctc taatacatcc tcaaacatcg tctcttcatc
    18301 aacagtcaac ccatcgcgtt caatagcgat atatcctttg cggtcaaaca agtaactgac
    18361 tgaacccgtt tctcccagtg atccaccgtt tcgagtaaaa gctactcgga catttgtcgc
    18421 tgtccgatta cggttatctg tcaatgcatg gactaagatt cctactccac ccggaccata
    18481 tccttcatac gtgatttcat cgtaattcgt ttcatctacg gcactcgtag cctttttaat
    18541 cgcccgttcc acattgtcgt taggcatatt tgcagctttt gctttgtcca caacaagccg
    18601 caaagctgga ttcatagatg gatcaggacc gcctgcttta gctgccatat aaatttcacg
    18661 agaaagtttc tgaaagactt tccctcgttt tgcatcttgg gcattttttc ttccttggat
    18721 attatgccat ttactatgtc ctgacatgat ttttcctccc ttgttctttt gagaatacac
    18781 ttctattcta gtactatttc acaaaaaaac aaagccacag ccaatctata aacgcttctt
    18841 gaatgccgca aaaagcacac ataatatcac agcagtcaga gcaccgatcg aatccaacat
    18901 gacatcttgg aacataggtg ttcgtccacc ggtcatcatc tgatggaatt cgtccaaacc
    18961 tgcgtaccct gttgctgaaa gccaagcaag gatcgctgtc agccaccaaa tttttagctt
    19021 ggggaaaaca cctaaaaaca agctgcctcc caaaataaaa taggtaccga aatgtgcgcc
    19081 cttccttaaa aagaactcta aaaatttggc gtagcccaga tggttgatac tcaccatact
    19141 tccaccataa ttaaaacgta tacccgctaa tgtgtcttta aatggctgat taggcagcca
    19201 gctttctaat cgcgaaactt gtgattgctg tccgtaagtt tgagaagaac tgtaaaataa
    19261 gattataacg actatcagtg cgattactaa ataaatattt ccgtttttca tttgtttttt
    19321 tgaagaattc ataagtcacc tctgtatttt atcttagact ttttttgaag aaacaacaat
    19381 cattttcata gagacttctt ccataaaaaa gtgctatact taattttgta ttacaatatg
    19441 aagggagttt tttcatgagt tattcattaa attggaatct agattctatt ttttccggag
    19501 gcagtcattc tgatgctttg aaccaacgga tgaagcaatt ggaagaccaa atgaacgagt
    19561 attatcaccg cgtaacaaaa tggtcaccta gttctgacaa tgcagagcag ttaaacgcta
    19621 ttcttcaatt gcaagaaacg attacgaatg gttttaccca atgcagcagc tatattactg
    19681 cattgctttc tgccaatgtc aatgattcag atgcaaaggt cttatcaggc aagctttacg
    19741 caatgctgcc tcgtctgcaa tcagccgaaa ctgtattgtc aaagaaattt gcagaaatat
    19801 ctgacaatga ttggaatcag atgctttctc aatcttcgtt tgaaacaatt gcttttcgtt
    19861 taaacgagat tcgtcgtgat ggctcacaat tattatcaga agctgaagaa aacattataa
    19921 atacgctttc tttagatggt ctaaatgctt ggagcagtca ttatgatacg attgtagcca
    19981 gcatttcgat tcccttcgaa caagatggac aagtagtcga attatctgct ggacaagcct
    20041 tcaataagat gatgggcgat ccagatccaa aagtcagaga aacacttttc gcagcatggg
    20101 aagaagcctg gaaagaaaag gcacccctct tcacggatac tttgaatcat ttagatggtt
    20161 ttagactttc tgactatgaa cttcatggtg tcacagattt tctacaaaaa ccattagagt
    20221 acaatcggtt gaagaaagaa acattgtctg tcatgtggga tacaatccag aaaaacaaac
    20281 aaccccttgt taattacttg actagaaaag ctaatctttt cggtaaagaa aaaatggaat
    20341 ggcaagatca agatgcacct attattttag gcgatttgaa agaaaaaacc tttagttttg
    20401 acgaagcagc agcatttatt atcgaaaact ttaacaaatt cagtccaaaa atggctaaat
    20461 ttgcccaatc tgcttttgaa aagagctgga tcgaagcaga agatcgtcct gggaaacgtc
    20521 ctggtggtta ttgtactgag ttaccagaaa caaaagaatc aaggatcttt atgacctaca
    20581 gtaattctgt taatgaggtt gcgacacttg ctcatgaact aggacatgct ttccatagca
    20641 gcacgatgtg ggatttacct tctttaaatc gcgaatatgc catgaatgtc gcagaaacag
    20701 caagtacatt cgcagaactg atcgtagccg atgctacatt aaaagtcgca aaaacaaaag
    20761 aagaaaaaat caatttgcta gatacaaaac tgcaaaatgc tttagctatg ttcatgaata
    20821 tccattcccg attcattttt gaaaatcgat tctatgaagc acggcaagaa ggattagtct
    20881 cagaagataa gatcacagag atgatggttg aggcgcaaaa agaaggctat caaggagctt
    20941 tagctactta ccatccatat ttctgggctg caaaattgca tttctttatt gatgacgtac
    21001 cgttttataa cttcccatac acatttggct acctctttag tttgggcatc tatgcttacg
    21061 ccaaccaaaa gggagcaaat tttgaagaag aatatatcca tttactacgc gatactgcct
    21121 caatgacgac ggaagatctt gcgaagaaac atttgaatgt cgatcttacc caaccagatt
    21181 tttggcaagc aggtatcgac atggtgactg aagatatcca aacgttcatg gaattgacag
    21241 aagaatttat ctgatttcta ttttatcaca ataaaaaaag agttagtcca gaatgggcta
    21301 actctttttg attaatcatt cttacatgct gacagcaaag tcaggggcaa accattgaac
    21361 tgaaccaact tttacgcttt cgcctggttg aggagcatgg atatattgtc ctccgcctaa
    21421 agcaatcgct acgtggtaag ttccgccttg tgacccccag aataacaagt ctccagcttt
    21481 tgcttgagaa actgaaattt ttgttccagc tgattcttga ggaactgtcc aaccaccgat
    21541 gtcacgacca gttacttgca tgtaaacata gcgtgtgaac cctgagcagt caaagccact
    21601 tggatcttta ccgccccaaa catatggagt accaatatat ttgtaagctt ctgctacgat
    21661 tgctgcacca ttcacacttc cagaaggagc tggagcaggt gttacaggtt ttggttgttc
    21721 aggtgttggt gttggcgttg gagccggagt actacttcct gtgccgtttc ctgtatcaac
    21781 actttgatct gttgatggtg ttgtaggcgc aggtgttgtt gattcttctg ttgttgaaga
    21841 ttcaggcact gttgttgatt cttctgttgc tgaagattca ggcactgttg ttgattcttc
    21901 tgttgctgaa gattcaggcg ctgttgttga ttcttctgtt gctgaagatt caggcgctgt
    21961 tgattcttct gttgaagatt caggtgttgt tgattcttct gttgctgaag attgtgtagc
    22021 tgatgatgat tcttcagtca ttgaagattg tgtagctgat gattcttctg ttgctgagct
    22081 ttgtgcagta gatgaagctt gagtagcttg tgcttcagcc tctgcttgtt cgcgtgcttc
    22141 tttttcagct ttttcttgtg cagcttgttg acgagcttgt tctgctagac gttgttgttc
    22201 acgaatacga gcttgctctg cttcagcttc tgctttttga cggttcaagt cagctttctt
    22261 gtcttcagca gttgcttgtt cagcagctaa tgaagttttc agtacgttca aatcagcttg
    22321 tttagataaa agatcgcctt tttgagattc caaagcagct tggttttcag ctaattcttt
    22381 tagtttagca tcgttttcag cttttttgtc ttctacagct tttttatctt gtttttgttg
    22441 ttccatcaag tcgttgtttg cttttaccat cgttgtcatt gcttgaacgc gtccaatagc
    22501 atctgctaat gaatcagcat tcaatacagc atcgatgtag tttgagcttg tgttgcttac
    22561 ttgtgcttcg cgtgcttgtt tttggattgt atcttcacgt ttttcaatac gttcttgtaa
    22621 atctgcaatg tcttttacta attgtgcaga ttcttgacgc aatgtatctt gttttgctaa
    22681 taggtcttgt gcttgtgtgt tgatttcaga aacttggctt tctaaggcat cgatttgtga
    22741 ttgtgcatct gcttgttgat ttttcaagtc tgcaatcttt tgatcttgct gttgtatctg
    22801 agaatcaaaa tcgtccgccg cggcagcgat cggtgatgct acggcagtca aggtcattga
    22861 acataccatt actgctgata ttaaactctt tttcactctt cattcctccg actggcttaa
    22921 tttaataaat atctaaatat atttaagtct ttcttaacga tataaaaatt gtaccatagc
    22981 agtgtgtcaa cgatataata gttatgttac aaaaatgttt caaaacaact tttaaaaatg
    23041 acaaaactgt tcgtttcaga aaatgatcat gaaaaagccg cgttaagtct agcatttctt
    23101 aagataacaa cgattctttt tcgctttcga cctaaaaata aaaaaagaaa ttattcatca
    23161 ctgaataatt tcttaaaggg aaaaatgaat aatacaaata taatcatgtt caacagcagt
    23221 gtaggtccta aaaaccttgt aataaaatac gtgttattta ctactgctaa tttaaaaatc
    23281 aactggacca ccatcgtaaa caattcataa cccgtgacaa agatgatcat actgaaaaat
    23341 tcagtaaaga tgttgacatg gataacgtct ttcattacat acatcaacca tacaattaaa
    23401 gggagagcta ctgcatagat accgataacg ccaatatagt aagcgtcata gatagccccg
    23461 agaacaatcg tggtaatcag taaataacgt ttttcaaacg ccatactgca acataataat
    23521 gccaatatca gaaagtgggc atttgacata taggtgcctt tcgaccactc ccctagcatt
    23581 cttgttaaat gaccatctat caacatcaaa aagaacaaaa cgattggcag atagtacttc
    23641 attgtttctt tcttcaacat ggactaatcc tccgctagcc gttggacaat cgtcacaact
    23701 gacacgtcat acatttctgc ataaggtttc acataaactt cccgatccag cccataacta
    23761 tctggtttcg ttttaataac cgtaccgatc ggcaagttag ctggagaatt ccctccaaga
    23821 ccggatgtct ggacaacatc cccttctttg atatccgtat caccagttaa ttgggtcacc
    23881 actaaggcat ggagcttttc atcatagttt ttaagcaaac caaacgcttc gccattagcc
    23941 gaagataccc gtactggaaa atgattggag ctttcattag aggatgacag taattcgatt
    24001 ttagacgaag ccgtattgac ctcgatcact cggccaatca atcctttttg cgacatgact
    24061 gccatatttg cttcgatacc gtctttagaa cccttgtcta cgatcagcat gtcctgccaa
    24121 ctgtcaggcg aacgagtaat gacatttgcc gttacttttt cgtaactagt cagcgtctga
    24181 ttcaactcaa gttctttttt caacgcttcg atttctctct cgtaattctt gttttgctgt
    24241 gaaacgtcat catagctatc gattttttct tttagccgct cattttcggc ataagttgaa
    24301 aaaagattat ggacagaatc tactccattt cgtacccacc gtactggggc agagattgtt
    24361 ttatcaacca ttgcaacact gtcattaacg atggattgga agaaattggg ctttccttca
    24421 ttcgctcgct gcgcagcggt cacacttagt atcgtcacca cgatgataac aataaccaag
    24481 gtaataataa tatttttatt aggattgaac ttcttcacaa agacacctcg aattaattag
    24541 actcttgtgt tcaattttat cactaaatgc cgtgaaacaa aaggtaagtt gctcctctta
    24601 agcttttttt tagcaaataa tagattctgt catttttagc ctgaaagaca aattttttta
    24661 tttgtttatg gatcaatctt tgctttgaat aactgcaatc ataccatctg aaaaagaaaa
    24721 ttctgcggta tctgaaagaa attcgttact tgcataagat gtcggatatt ctacttcttt
    24781 attttccaat acatacttgc tgccttttaa tgtaagattc gagacgggag tcaagcagca
    24841 ataagctaaa tacttcatat ctggtatctg tcttactata tgctctccag gcaaataata
    24901 agacaaataa ttttgacaat ctctgataat aagctgatgg gcaaagggtt tgaagcgagg
    24961 ttccatacct aaccagagat tggctaaaag atgatccaat cttccgcccg tagctccaat
    25021 caaagtgact tccgcttgcg ggtaacgttc aaatgttttt aagataccta actgtgtatc
    25081 tgtatcgtct ttttctgcag gggaacgggt atactctttg gcttttgcaa atacttcctt
    25141 ctgctcttcg ttcgacaatg aatcaaaatc acccactgcc agatctactg ggtagcctgc
    25201 ttgaagcagg tacagcgcac cacggtctat cccgacatag gcatcgtatt tggacagcag
    25261 agactgcggc cagttttgcg gattacctcc agcagctaat aaaattttca tttagtctaa
    25321 tgcctctttt aatcgttcga tttgtccgac tggattttcg gctccataga tatatgatcc
    25381 tgcaacgaag acatccgccc ctgcatcttt acagattttt gctgtttctg gaacgattcc
    25441 gccatctact tcgattgcat aagtccaatt gttctctcta cgtagatcag cgagttccgt
    25501 aattttttct actgtttctt caataaagct ttgtccgccg aacccaggat tgacagtcat
    25561 gactagtact tgatcagcca aaggcaaaac atgtttgatc atggagactg gtgttcctgg
    25621 attgataact actccagctt taactcctaa gctttttgtc atttgaagtg cccgatgaat
    25681 atgaggagtt gcttcgacgt ggacagtaat gatatctgct ccagcttttg caaatgctgg
    25741 aatgtagttt tctggatcaa cgatcatcag atggacgtct aacggtaatt tagtaacagg
    25801 acggattgcc tgaacaatat ttggccctaa tgtgatattt gggacaaatc ggccatccat
    25861 tacatctacg tggatgtaat ctgcgcctcc tttttccact aactcaatgt ctctttgtaa
    25921 attggcaaaa tctgcactca aaatggatgg tgctatcttc attcttgttt cctcctattt
    25981 tcgtttacct tctttttttg catacattgg ttttcttttt tcgatttctt gtaagaattg
    26041 caaataattg tcgtaacgtg tctgtgcgat accacctgtt tctactctat gttttacttc
    26101 gcagccaggt tctttagcat gcatgcactc tctaaatttg caatggacag aagcttctac
    26161 aaattcagga aattgtctcg gcagttctcc tgcttcaatt gtcaaaaagt caatcgagct
    26221 aaatcctggt gtatctgcta ctaacccgcc atagatagga atcagttcca catgacgtgt
    26281 tgtatgccgt cctcttccaa gtgcatcaga aatctctcct gttgctaaat tcagttccgg
    26341 tgatatttga ttcagtagcg tcgattttcc tgctccagac tgacccataa agaccgatag
    26401 ccgttctggg aagtaacgga tcagttctgt cgtatcaccc gtatgattgg gtacaatcac
    26461 cggatagcct attttagtat aggtttgctt gatctcttcg attttttctg ggtcgtctga
    26521 caagtcggtc tttgtcaaaa aaataatcgg ttcgatcgac tcgtattcca aggtaactag
    26581 aaagcggtca agtagattat aagaaaatgc aggctctacg atacttgtga caatgattcc
    26641 aagatcaaca tttgtgactg gtgggcgaac taattgattt tttctcggca agatttccag
    26701 aagatagccg tctgtttgat tttcactctc aaagatcacc tgatctccga caagcggcgt
    26761 gattttccga ttccggaaat ttcctcttcc tcttgtttga taggtctctc cgtttgcgta
    26821 tacatagtaa aaaccgctga ttgcttttct gatttgtcct ttgataagtg ccacctccgt
    26881 ttttgtttat tttaccatac gaagaggaaa gtaggtgtta ttccatgcaa acttctcaaa
    26941 gtatatggct tgaaaattga tcaaaagaga tagaaacaca aaaaagagaa tgcaacaaat
    27001 caatgtcgca ttcccttctg atatatttta ttcagcactt attttgaact ttctgaggtg
    27061 ctagctgatg aacttgtgga gctggaagat tcttcttttg aagagttgct tgtgctactg
    27121 gagctacttg agctgctaga actgtctgaa gagtcttttt cagaactaga agacgctgga
    27181 tctggtcctt tagagtaaat tacgttcaca accattcctt tggtaattgt cgttcctgcc
    27241 cctggtactg tacgaatcac tttatcttta tccaccgtgt ttgaatattc ggaagtctca
    27301 tgcccgatat attctgcacc gacactagct aaataacttt gtgcttcgct ttttgtatag
    27361 ccggagatat cattcaattt aggctctgtt ccttggctta cgacaaaatt gatgtaagtt
    27421 tcttctgcta caacttcacc cgaggctgga tcctgactga tgatcgaacc tgatggtaca
    27481 gtgtcgctat agtcataagt agcactaata ttattgatat tgaatccttg tgtaacaagc
    27541 caattacgaa cagtttcgta gttttctcct acatgactac tcaaagtcac tgtttctggt
    27601 cctttgctga ctgtgaaagt aatcgtgtct gttttaggat cgacttctgt atcttctgat
    27661 ggagattgcg aaatgatcat tccgctttct acctctgaac tgaattcctc ttctttttga
    27721 atcaagtcag cagaaaaccc taattctttc aaacttgctt cggcatcttc ataactttta
    27781 ccactgtaat catctaattt gatttttttc gtgccgctac taatatacag agtgacttcg
    27841 cgtttttgct tgactgtcgt tcccgctgcc gggtctgtct tcactacatt accttcttcg
    27901 attttgtcat ctgcaatttc ttctgttttt gttgctactt ctaatttcgc ttttgcgagt
    27961 gtttctctag cagaagcttc agaaagatca gaaacatcag gaacaacaac ttctcctcgc
    28021 ccgccagaag caaagaatac agctgttccg attcctaaaa cagcaagaat agctaatagg
    28081 atcagccacc attttttctg tcctttcttt ttaggatcag gcagaacttc ttcctcctct
    28141 ggtaaggttt cgttttctgg cttgtctttt ggcagaggca tagatttgaa tgaatcaggc
    28201 attggtgcat cttccgtaag gggtgtgata acttttgttt cattatccat cacatgaggc
    28261 tgccattttt cttcgtttgc acgagctggt gataatactg tagaaagatc atcagacatt
    28321 tcctcggctg acttataacg gtctgcaggt tctttagccg ttgcccgtaa aatcacgttt
    28381 tccagtgctt gtggcacatt tggatcaagt gcttttatag atggaagatc atcttgaaaa
    28441 tgttttaaag caattgttac agcagattca ccatcaaacg gtacgctacc cgtcaacatc
    28501 tcataaagga tgatccctaa agcataaata tctgactgct tcgttgccat acttcctctg
    28561 gcctgctcag gggacagata atgaacagat cctagcatcg tattcgtctg cgtgatcgat
    28621 gtttcagata aagcgatagc aatcccgaaa tccgtgattt ttacaacacc ttcattatcg
    28681 atcagtacgt tttggggttt caaatctcgg tggatgattt gatggttatg agctaaagaa
    28741 atagcagaaa ggatctgctg cataatattt actgctgttt cataaggaac aggataatgt
    28801 gtttggatat atcgtttcaa gtccattcct ttgacgtatt ccatcaccag atactgcatg
    28861 ttgtcttctt caccaacatc gtaaacacta acaatgtttg gatgaacgag ttctgttgct
    28921 gctaaagctt ctctctgaaa tcgtcgaatc gcagtctggt cattttgaaa gtcaaatcgt
    28981 aagactttaa ctgctacatc acgatccaag attaaatcgt gcgccaaaaa tacatttgcc
    29041 ataccaccgc taccgatatt tccagtgatt tgataacggc catttaattt tttgcccaac
    29101 tcgatcatac actgtcctcc ttatattcga taagaagaac ggtgatatta tccgctccgc
    29161 ctgcttcatt ggcacgatca atcagctcag ttactttatg agacaatggg ccttcttgat
    29221 tgatgatcgt tcctatttct tcttcagaca gcatgttcgt caagccatct gaacataaca
    29281 gcagtcgatc ttttagttgc caaagatgag tagaaacatc tacttcgact gttccaggca
    29341 tgccgacaga acgtgttaaa atatttttcc ttggatggtt cactgccatt tcttcgctga
    29401 tttctcctga tttgactagt tcattgacta aagaatgatc ctccgtcaat tgcatgattt
    29461 tatcttcacg aatcaaataa gctctgctat ccccaacatt ggcaatcgtg aaccagtcat
    29521 ctgacagagc ggccgctacg attgttgttc ccataccgtt atattccggc tgttcttgtc
    29581 cacgctgata aatcagcata ttttcttctt gaatcgattg aatgaaccat tgggcgattt
    29641 tttcatcgtc aacagcttct tgtttttccc aagcttcgcc tagattcgtt accgccatct
    29701 gactggcaat gtctccggca cgatgaccac ccatcccgtc tgctaataca gcaagcttga
    29761 gacctgaacg gctggtaaat acactggcat agtcttgatt tgtgttacgt cgtcttccta
    29821 catctgattg atattcaatc tgcatttttt cacctcatta ctttttgcgc aaacaacaaa
    29881 tgaaaaatcc atctgtgtaa aactgatgcg gatacaaagt caacattttc tcttcgattg
    29941 atgattgtag cataggattg gtcaaaacat caatcctctc aaaatcaggg tgtctttcca
    30001 agaaagcaga aacaacgtct tggttttctt cttttaagat cgtacaagtg ctataagtca
    30061 gaataccgga agactttaaa gttggtgcgc agctttctaa aatttccaat tggatttttg
    30121 gtaaattcgc taattcatcc ggctgttttt tataacgaat atctggtttt cgccgaagca
    30181 gcccaagacc tgaacaaggc gcgtctacta aaatccgatc aaactgctct gcttcgaact
    30241 gtttactgac ttctctagca tccatctttt gagcgtagac agcagaagag acacctagac
    30301 gtgctgcatt atcttcaatg agtttgactt tgtgttcgtg tacatctaaa gcgaccactt
    30361 ctccaccgca tgacggatct aaaaaagaag caatatgcgt cgtcttacct ccgggtgctg
    30421 cacaggcatc taagacatga tgttcaggct cgatttgcat agacggcgct acaagcatcg
    30481 aactttcatc ttggatcgtc atgacgccat cgtggaacaa tgagctagaa gctaaatgcc
    30541 cttttttcgc tactataccg tatggactga cttggctttt ttcagctgtg atctcttctt
    30601 cttgcaaaac agtgatggcc tgatctctgg aaagtcggcg tgtatctact cgggcactag
    30661 catggctagg ctgatatagc gataaaccga gcttttcagt ttcttcgtat ccgatttgat
    30721 ccaccagtcg tttcgtcatc catagaggca ggctgatttt gacagaaagt ctttcgatag
    30781 gatcagcaat gccttgcaga tctggtgctc cttgacgctg ataattcctc aagacaccat
    30841 ttacaaattt ccctgtccca ggatttcctt ttacttttgc gatttcgacc gcctcgttta
    30901 aaatagcatg tgcagggact ttatctaaaa attctaattg gtatacagaa agataaagaa
    30961 gcatcttgac ccagtcatcc actttttttg ctttagcgat caatggtgca acgtaaaatt
    31021 ccaatagtcg ctgatggcta atcgtaccat agatcaattc tgtaaataaa cccacatctt
    31081 tttgatttag ctcgctttgt ttgatcatct cattcaacaa aagattcgag taggcgccac
    31141 ccttgttcac tcgctctaat gtcagtaatg ccatatatcg tactgatttt ttcatttttt
    31201 tagggatttt tttcattatt tcacctgctg accaatctga acttgctgac cgcttccgtt
    31261 aaggaaatca tgtacggcct gttttccttt accagctggc tgtaattcat tgatggctaa
    31321 gacggtctgt ttaccgcaag caatccatag attttttttg tctttccgga tgatcgtacc
    31381 aggtgctttg tctgtagttt ctgccaatac ttcaaccgat aataatttcc agcgtatgcc
    31441 ctcataggtc gtgaatgcaa ttggccaagg acgcattccg cgtacttggt tgtcaatttc
    31501 ttctgcagtt ttatgccaat cgattgcttc ttgttcccgt gtgatgtttg gcgaaaaagt
    31561 tgcttctgac tcgtcttgag gtactggttt ttgtccatcc aaaatattgg gcaatgtttc
    31621 tagcaatagc tttcttccta aaaggctaag cttttcaaac atcgtcccta catcatcttg
    31681 tttagtgatt ggtatgcttt cttgcgcata gattccgcct gcatccattt ttttgatcat
    31741 ttccatgatc gttacaccag tttctttctc cccattcatg atggcataat gaacaggcgc
    31801 acctccccga tatttaggaa gtaaagaagc atggacattg attgccccta attttggtac
    31861 ttgcaataac ttttcaggca agaattggcc aaatgccgca gtgacgatca catcaggcac
    31921 caattcaatg atttcttcca tttctggcga accggagatt ttctctggct gcagcacacg
    31981 gataccgtgc ttcagagccg ctttttttac cggtgtcggc gtaattactc gtttccgccc
    32041 aactggacgg tcgggctgtg ttaccactgc ctggatctcg taacctgatt ctatcagccc
    32101 ttcaagaatc ggcacagaaa aagcaggtgt gcccataaat actagcttag tcatcaagat
    32161 gttcctccat atattcttca agttcttctt ccgggatctg ctgtatcatt ttctcaatga
    32221 acaagatacc gtctaagtga tcgatttcat gctggaaggc acgggctaaa tagccaaaag
    32281 ccgtcacttc tatctcttct ccgtctcgat cataataacg cacggtcact tcatctgctc
    32341 gcttgaccgt tccatagaca tgcggaatac tgagacaacc ttctacgtca aggctttcac
    32401 cttttgcttc gatgatttct gggttaatca gttcaaattt ctcgccttca tctacttcga
    32461 tcacagcaat tctcttgttt tgtcctactt gaggagcagc gatccctatt ccgtcattcg
    32521 caatcattgt ctcatataaa ttgtctaata aagcgattgt ctcgtccgtg attacatcga
    32581 ttggctgagc agttcttttt aatttatcat tagggtggat caatatagga tagcgcataa
    32641 tttggccctt tcttaaataa agtttaatgg ttcgttgtca attgccacaa aaagaccttg
    32701 tcgctgttct ttctggcttc cattcaaaat ttcatccaaa acatgaggaa gattcggttc
    32761 atgtttatat ttgatgatta gttgataata atatcggttt ttcactctca aaatcatgct
    32821 gggcgttggc cctaatagca agctttgagg gcttaacact tcttttagct gattggcaat
    32881 ctgaaagatt ttctttgccg ctacttgttc ttcaggatgg ctggcggtta ttttcacagt
    32941 aaagtaataa ggaggatagc ctccacgatg ccgtacatgc atttcctgct gataaaactg
    33001 ctcataattt tgttttttag ccaattctat ggcgtaatgt tgaggattga atgtttggat
    33061 cacgacttcg cctgcttttt ccgctcgccc tgctcgtcct gaaacttggg taagcaattg
    33121 gaatgtatgc tcactagaac gaaaatccgg taaattcagg gaggtatcag cattcaacac
    33181 cccaactaat gtcacttctg gaaagtccaa acctttagca atcatttgtg tccctaataa
    33241 tatatctgct tcttttgcac cgaatttttg taaaatctgt tcgtgtgccc cttttcttcg
    33301 agtggtatct acatccatac gcaaaatcct agcttgcgga taaagttctc ggagttcctc
    33361 ttctactttt tgagtacctg ttccatagta gcggatttta ttgccgccac aattaggaca
    33421 gcgattggga ataggttctt catgtccgca ataatggcag cgcattgaac gtgtatccat
    33481 atgcagtgta agagagatat cgcaattagg acatggaaga acaaatccac agtccctaca
    33541 catcacaaaa gacgaatatc ctcgacgatt gagcagtagg acagtttgtt cttttttgtc
    33601 taatcgattt tgtatttttt cctgcaaatt agcagaaaaa gtagaggtcc gatgattctg
    33661 aaactcttca cgcatatcta cgatctcaat agctggaagc tgtgcagctg ctttggctcg
    33721 gtggtttaac tgcaataact gataaacgcc tttttgagct cgtgctcttg attctaaaga
    33781 gggcgtcgcg cttcctagaa ctattgggca atgatgatat ttactgcgcc aaattgctag
    33841 atctctcgca tgataacgcg gtgtctcatc ttgtttgtaa cttgattcat gttcttcatc
    33901 gataatgatc agcccgatat ttttcagcgg ggcaaaaata gcagatcgtg cgcctactac
    33961 tacttgcgct tcttcccgct caatcttgcg ccattcatca tatttttcac cttgggaaag
    34021 tccgctatgt aagaccgcaa ctgattctcc gaagcgtcct ttgaatcgct cgaccatttg
    34081 cggagtcaat gcgatttcag gcactaacat aatagccgtt ttattttctg acaaaacatc
    34141 agcgattgcc tgaagatata cttctgtttt cccgctgcca gtgattccct ccagcaaaaa
    34201 gacctgatct ttttgctgct gacctgctgt gatgatttgt tcgacagcat ttttttgttc
    34261 tgcattcagt tccaatgccg tcgtttggtc aaatactcgg tctttataag ggtcgcgata
    34321 acgttctgat tctataaact ccagccagcc cttttctttt ccttgattca agacagcagt
    34381 actgattcct ttatttttga aataagcaat tggttcttct tggctcagtt cagataaaca
    34441 caaaagaagt tctttttgtt tcttggcatt ttgccgtaat tttgcccatt cttcttcgat
    34501 ttgcttttca gttaacaaag aacggaccaa acgaatcgtt tttactttat ttcgtgtatg
    34561 gacttcatat ttgatatcta ctaattgctc ttggcgccag cgaatcaatt gagaaaggct
    34621 gccttcttct tctgcttcct tccaggaaat cacttctcgc tgatcaaaca atacctgaac
    34681 ttctggtgtg tctgccaaag gatagatcaa tttatcatat tctgctttca tcacactcgg
    34741 aagcatcgtt tgtagacaag tgattttgaa tgcgtaagta atttttttca tataatcagc
    34801 taatgaaagt aattcttcat tgaccaccgg agcaaggtct agcaaacgaa taactgcctt
    34861 caaagatgga ttagctgatt cttctgattt ttggatagcc ataacaaatc cttggacatg
    34921 acgattgcca ttaccaaaag ggacttctac tcgcatcccg acttgcaaag ctgtttccat
    34981 ttcagaggga accaaataag tgaatggctg atcggtctgc atagtaggta cgtccacaat
    35041 gacttcagct gttaatgtca tggttcgttt cctccttttt ttgtcttttc ttatcaagcg
    35101 acaaaaagtt ccttgcctat tttactagag atcgatggat ttgtcttatc ttctgccaaa
    35161 tcctaacaaa aaattaacag cagataagaa aaaaaagaac tgtgtcaaaa agttcagaca
    35221 cagccttttc taccagacaa taagtggcga atgtttgaaa aggaaacagc tgggtttgct
    35281 tttcttttta gatgttcgct ctatttttct ttattacatt ttttgctctt cacggatacg
    35341 tgattccaat tcgtgttgtt cacgctcttt ttgtgcttga cgttcctctt cttccatctt
    35401 caaacgttca cgttttaatt caggatgtgg atcattgact acatttcctg catcgatttc
    35461 ttctaaagct tgaccgacgc tttttactga atcaaaagaa tctagagtag gctgtgcact
    35521 tgcatccaat tcatgcgcgc gtttacttgc taaaatcact agagaatatt ttgaatttac
    35581 acgatctaat agtgagtcaa ttgaaggttt taacatcata agactacatc tcctttaaca
    35641 ttttaatata tttaccgatt acgcgatcta ctcgaaaatg ttcactggca ataatgtttt
    35701 tgattcgttc aactgctagc ggcacttcat catttacaac agcatagtcg taaagagcca
    35761 tcatttcgat ttcttctttt gcaactttca ttcgtttttc gattacttca tctgcatctg
    35821 ttcctcggcc tacgatccgt gattttaatt ccgctaaatc tggcggagtc aaaaagatga
    35881 atacaccatc gggcactttt tctttgactt gttttgctcc ttggacttca atctccaaga
    35941 aaacatcttt tccttcatcc aatgtcttgt tgacatatga caaaggagtt ccataataat
    36001 tcccgacata ttctgcgtat tccagcatct ctccagcttc gatcattgat tcaaactctt
    36061 ctttgcttcg gaagaaataa tcaacgcctt cgacctcccc ttcacgcatt ttacgggtag
    36121 tcattgagat cgaatactga aaatcattat cctcactctc aaaaattgct tttcgtactg
    36181 tgccttttcc tacaccggat ggaccagaca acacgattaa taatccacgc tctgacatga
    36241 cgacgtcctt tcaactagtc tttttgtact tatcgtttat tttgcacttt ttttctgcaa
    36301 ctttcaaggt tatctttaat tatagctaga aattggtaaa aaagctaccc tgtaccaaga
    36361 ggtcgtaaaa aagctgtcct gcatcaagta ataagaacag ccaaacataa acagcttctc
    36421 atgtttttcg tttggttggg gttattatcg cagatgcaag cttttgaacg ccgtgtataa
    36481 gaggtcgtga agaagccgat tagtttcgag tatgaagaaa aattttgtga aaatagtttc
    36541 tcacattttt gcataaattt ggcttaatac cgagaaaatg gtttttgaac atcacttatt
    36601 caaaataaaa aaagattgtg acacgaattt tgtcacaacc tttaaatgta ttcctagagc
    36661 aaagaagcta agcgaagaaa tccaattctg tttatttatt gtatttcttt gttcagcatc
    36721 cttgctaaaa gcccatattg gacaaagtat tcactacacc catgatctca tcttttcgag
    36781 aacgtgcttt ttctgtcgga tcttctacct cttctgtgtc aattcgaaca ataaatttcc
    36841 gattcatcgt cacgtaagca ccgtcgttga tcaaaataaa cagttcgttc tcattatagt
    36901 ctgcacaggc atctttccat acgtcaaagc cgtttttttc agaagggata tcccgtttaa
    36961 ccacagtttt tccacttaat tcgtggaacg ttacgtcata atatttcatt atctctacct
    37021 ctttcgtcat tcaatatttt gtacttgttc tctgattttt tctaagatcg tcttagactg
    37081 gacgacttga tttttaattt cgattgcatt tgatttggaa ccgatcgtgt tgatttcccg
    37141 attcatttct tggatcagga aatctaattc ccgacctaca ggacattgtg cttcgataag
    37201 ttcatctagt ttttgtacat ggatcgccag acgatccagt tcctcgtgaa tatctccgcg
    37261 ttccaacaag atggctagct ccgtcaacaa ccgttgctca tcgactttcg ctccaagaaa
    37321 ttcctccagc tttttttgat accttatttg aaattccttt tcatagattt ctacaaaact
    37381 atcgagtttt tttagtactt ctttgaattc ttcagtattc tttttcaaca ctaaagctaa
    37441 agcttgacct tctttttctc gactcgcctc aatagaatca agtgcgcttt tcaatgtatc
    37501 tagagctaac tgtgccaatt cttccatgct ctcttccggt ttttcttcta ccgttacgaa
    37561 gttttcgtcc agcacgaagc gttccgccat ccgactaatt gacacttgat tttgactacc
    37621 gtatcgttgt tgaattccgt ctgtcaattg agtgataagt gaatcaatca attcccagtt
    37681 gatgacgatc tgtttttcgt ttttgccaat ataagctaga ttcacaaata cttcgattcg
    37741 accgcggctt aactgctgtt taataagctg acgaaaatcg ttttcaacaa agttcaggat
    37801 tttaggactt cgtatttgaa catctagaaa acgctggttg acacttttta tttcaatatc
    37861 taactgatac gcttttgttt cgcggatggc tttgccaaaa ccagtcatac ttttcattcg
    37921 acagggtcct tcctcattac atattttcta ttgctttttg cagttgttca aactcttcgt
    37981 tcgtcaatgt caggcctttc cccatttttt catggtctgg cgcccaatct cgaagatcga
    38041 attttggcgg gcgaccattc cagctgacta ggttcagttc tttacgccac cccttgttgt
    38101 tttctgacaa tatagcgatt tcttctacga tttcatatga aaattcttgt gccatggttg
    38161 ttcctccttg agataatata aatataggat cagtaatgtg tcaactggtt gcttaagcaa
    38221 ttcatgctgc aaacgatcct tgcgttttcc ttgtagcaga taatcaaaat aaacagtttt
    38281 cgtgcgctca ttttgtattt tttgctggta ttttcgatac cttcgccatt cttcagtact
    38341 taattgacca atttgtgcca ataaatcatc cggcatactt tttatatctt gttggcagat
    38401 catttgaatc atcatgtcta cttgatagat ttcattgggc agtaaaaaat gtttttttgc
    38461 taaaagttct agcatcgcca atccgcatgt ctctggtacg accctgttct tatataaatt
    38521 acgcagtttt tgccaaaatt ttatgctttt gaaaaaagat aattctaact gaatttcttt
    38581 tgttaagtaa gacaaaaatc ctcgccagta tgttcttgca ggtttaacag aatgaaatgt
    38641 ataccagttc ccatagtaat agcctaatcg ttttttcatt tccagatagc ctaattgtaa
    38701 atttaattgg ttcctttggc tatcaaaaag cagaaagctg cctagtgtcc acaatgtttg
    38761 acatttccaa tggataaaaa tgtctggcat acgaattttt ttcgctgggc ctggcccttg
    38821 cacatctacg acaaatgcct ctgttgctcc tttgttaatc gcaatatcaa taggtatttt
    38881 gttgcgatac ccaccgtctg catatttatt ttttccgatc tttcgataag ccatagcggg
    38941 ataaaatgaa gcagaggcga gtatccaatc tggtatttct tctgtttttg tctgttggat
    39001 atcagtcact acttccgcta gttctggcag tttagtagac accgtatata aacgtatttt
    39061 gggattatgt ttgagtaagg acaaatccaa attgtctttg accatctttt ccaatggagc
    39121 agaagaaacc ccgccttcaa ttagtgctgt tttcgtcatt tggcgtcttt ctcgatagaa
    39181 gcgttcacga aggtcttcaa ctgcagccat ttctggtaac gcaagcactt gtcttgtcga
    39241 cagctttttc cataaattca cagctttttt catatctcct tgtaaaatca atgcgccgtt
    39301 caacgcacct accgaggtac ctgtgataat ctcaaagttg atatcatgtt ccttcagcgc
    39361 ctgccacaca ccaatctgat aagcaccgtg cgctcctcct ccacctaaaa caagtgctgt
    39421 atgatagacc agctttttat ttaattgtgt ggttccttga tagccgtttt tatgaaaaat
    39481 acgcaaccat tcttctgata gaggagattg aaataaaaaa caacagtttt ctttaataaa
    39541 aaaacgagcg caagcttcca tataccgaaa aaattttttc cattcaaaag tttcaccttc
    39601 aatcaacaga ttattaaaaa taacatctcc tctactttta gcaatagtaa aatagacggt
    39661 atattttttc gatactgctt taaaagcaca ttgctttttt gcagaacgcc aaaaagcgtc
    39721 tctactttct tgatgacttt gaaaaaatgg aaggggttgt tttgcttgct ggataggcga
    39781 atacagcgac attttctgtg aaaagtaaac tttttttatc tccatcgcgc tctccttttt
    39841 cttcaagtat accagtacgt cataaaaagg tcatgagtct cgtattcgac tttgtagatt
    39901 ccataagaaa acaattcata aaacctcctt taaataaaaa aatacaaaaa tcacaaaaat
    39961 taattgaaaa tgagagtgtt ttcaatgaaa acgtgatttt gtaatggaat tttaaaataa
    40021 aagaaacaag cttttttaat gcaaatgttg taaaataaaa gagattaaga ggaaataaga
    40081 ggaggaacat gatgactgag tcagtttatg tacatattga taccacaagt aatgctgttt
    40141 taacaaaagg gctgactgct actgactttg caaacagtat cgtgcatttt cctcaaaact
    40201 tgctcttgct tgatccatct gcttctgctg gagaatatga atcccataca gggctgaaag
    40261 taatccgtgg tacagaaaat attcagcgtt tcttttcctc ttctcgtaac aggcgtggat
    40321 tcgatgaatt gaagtggatc gactttactg atttaacgat gctgaaagaa ttgacacctt
    40381 tagaaatttc agaattatta tattttggtc atatgaaaac acatcttcat tcgccgtttt
    40441 tttataaact acagaataat tttgtatact tcgatcttaa tgatcagctg aatcggattt
    40501 attatcgcta tttagatgaa ttttatagga tttttgctag taaattaagt tcgatcttgt
    40561 cagataagat caatgtaaaa aaatcttttt tcaaaaaaga aacacctgtt gaaaaaatca
    40621 gtgtggaatt attgaaagaa atgcgtaatt tgatgcagga agggatcatt ttttcttttg
    40681 atcaagttgg tttgatcgat ggtaaatatc gtatcccaat ctatgtgatt gaagattata
    40741 tttggaaatt aaaaggcacc cggtatcgag aagaggaaat catcgctact ttaatctact
    40801 ctgtcaccga tcgacactgg caattggtag aagatgccga tctttcttat ctgccattga
    40861 agtagatcta tttcttatca aaaaataaaa aggaaacaag aaactaggac atgattctgt
    40921 ctcaagtttc ttgcttcttt tttatttaca gctcctgtcg tttatctggc acaaaaatcg
    40981 aaggagtcgt ttgttggatc cctaataaaa cgatgatcgt tacaatagct gtactcaacc
    41041 cgctggcacc attcattacg aaagaaaaga gccaaggatt catcccccat agtgcatacg
    41101 ctccccagaa aaccactcct gcaataaaat gccaaaaata tctggaaagt gcgccaatca
    41161 atgcaccaaa cacgatgctt tttctaattt gtttcgtatt atcgtctaca tagccttttt
    41221 ggacatttga agccatgagc cctgctgcgc ctgcaaaagt ataggcaatc gggtactcaa
    41281 tcaacacttg aacgacactt aaataataaa cttgtcccaa cggaaaatgt aggatgcccc
    41341 aaatgaaacc tgccagcatc cctgcctttg tccctcttct taacgcaaaa agagtcaatg
    41401 gaatctgccc taatgaaata gaaaaactgc tgccgatctg gatcggaatc aacgacaaca
    41461 ccatcgctaa cgctgcaaca atcgttcctt ctacccaaac tctatttttt cccataaaaa
    41521 aacttcctcc tttgaataaa aaagtcacaa aggaggaagc agctttcgaa aatagtcaaa
    41581 cagctgttta ttcacaattc ctacgctcgc ataaccgata caggtacgaa gggtttagaa
    41641 atagattcaa tctcagccca attggactcc cctttgtgag tttatttatt atttattttt
    41701 tgtttcaaac aatggactaa ctggtttgtt ttcatggata cgtttgattg catctcccat
    41761 tagtccgcca acacttactt catcgatttt gtcgatttta cgatcttctg gaagatagat
    41821 cgagtctgta acaactagac gctcgatagc tgaatcttct attctttgta aagcagggcc
    41881 ggataatact ggatgtgtac atgaagcata cacactagtt gcaccagctt ctttcaaagc
    41941 attggcagcc aatgtgatcg tacctgctgt atcgatcata tcatcgatca gtacacaagt
    42001 tttgcctttg acttgtccaa tgatattcat tacttctgca acatttgctt ttggacggcg
    42061 tttgtctatg atcgcaatcg gagatttcaa aaattccgct aatttacgtg cccgtgtgac
    42121 accaccatgg tctggtgaaa caacgacaac atcatcgcct ttgattcctt tttctaagaa
    42181 ataattggca ataagtggcg cacccatcaa atgatcgact ggaatatcaa agaaaccttg
    42241 gatttggact gcatgaaggt ccaaagtcag cattcttgtt gcaccagctt tttgaatcat
    42301 gtttgctact aatttcgctg tgattggttc ccgtgcacgt gctttacggt cttgacgcgc
    42361 atagccataa taaggcatga ctacgttgat tgtttttgca cttgctcgtt tcagtgcgtc
    42421 aatcatgatc agcaactcca tcaagttatc attgactggg ctgctcgttg attgaataac
    42481 atagacatgc gatccacgaa tactttcttc aatgtttact tgaatttctc catcactaaa
    42541 ttgagtaaca gatgattttc ctaactcgac gccgactgct gctgcaattt tctctgctaa
    42601 tgggcgattg gaatttaacg caaaaatttt taatcttggg tcaaagtaat gttttgacat
    42661 ggggacctcc actttctttt tcacaactct agaattctac ctaataaata gtagtcatat
    42721 tttttaaata aatcaagtga ttttgcaaaa gtcttagttt agataaggga gtttttttgc
    42781 atatccttct ttattgactt gtcttgctct cgcaattgcc agcgcatatt ctggaatatt
    42841 gtctgtaatt gttgacccgg cagcaatcga actgttttta gctacctcga caggtccgat
    42901 gatattcgtg ctagaaccaa taaagctgtg gtcgccgaca gtcgtatgat gtttgttctt
    42961 tccatcatag ttgacgaaga caacaccaca tccgacattg atttcttctc ccagcgtagc
    43021 atcaccaaca taagtcaagt gaccgacttt agttcctttg ccgatttgag catttttcac
    43081 ttccacaaag tttccgatat ggacgttttc gccaatttca gcttttggtc gtaaatgtgc
    43141 ataaggtccc acatctgcat gtttcttgac atggctgctt tcaatgactg agttttcaat
    43201 gacgacgtga tcctcaatcc tactgtctac gatttttgaa tgtgacccga tgacacaatc
    43261 agaaccaatg actgtatttc cttggatttg tacacctgct tcgatcaatg tgtctggacc
    43321 gatttctacg cctgcatcga tgtatgtcgt tgctgaatcg acaaagctta cgccattgac
    43381 catatgcatc tggttaattc gctttctcat gatctcattt gctttagcta acgcgatccg
    43441 atcattgact cccattgatt cttcaaaatc atccgtttga taagcagcaa ctgtatggcc
    43501 ttcttctttt aagatttcga tgatatctgt tagatagtat tcaccttgag cattgtttgt
    43561 tccaactttg cttaacgcat cgaacaaagc ttcgttgtca aaacagtaag ttcctgtatt
    43621 gatttcttgt acaagagctt cttctggtgt tgcatctttt tgttccacga ttttttctac
    43681 gatcccaata tggtcacgga tgattcgtcc atatcctgtt gggttttctg cttgtgccgt
    43741 caagatcgtt gcacttgcat tttttccttg gtgatattca aaaagattgt tcagcgtttc
    43801 agtagtcaat aatggtgtat cgccactgat aactaacgtt gttccttttt ttccttccaa
    43861 gaaagaagca gcttgcaaaa cagcatgtcc tgttcccaat tgttctgctt gtaacgcata
    43921 tttactgcgt tctcctagtt gtgctttaac ttgttctgct ccgtgtccaa cgatcgtaat
    43981 cacttcatct ggtttcgttt cactgacccg attaatgata tgttctacca taggtttacc
    44041 agaaacggga tgtaaaactt tatataattt tgacttcatg cgggtacctt tccctgccgc
    44101 caaaataatt gcataacgtg cgtccaactg cgtcactcct aaatatagtt tgctgatttc
    44161 agtttatccc agtataccca ttttttcaac aaaacaagtg attttctttt atcccagcgt
    44221 tttctggata aaaaaataga tagaccataa cgggcctatc tatttggtaa ccggtgctct
    44281 cctgccaact cctcggtcac aagtcacact actcataaaa catgaaaagc tgttttttga
    44341 aatcgtttct tgtgacttag agttaaacgg taggcttaca acctcttcat taaacgtttt
    44401 tcaatcagct gaccttcggt attagctcaa taaacggcaa aatatgagga gcaatttttc
    44461 cgtttattga gctaatactc aggacaggac gctgatttca caacctctag ataaacggtg
    44521 ttcaaaagcc agtttctcgg tattaagcca aatttatgaa aaaatgtgga aagccatttt
    44581 cacaaaattt ttcttactac tcgaaactaa gcggcttttt cacaacctct tattctgaaa
    44641 aataatttcc tggcacaacc gtaatatttt tcgtttgagt atctacatct ttgacataaa
    44701 gcagtgacgt ataatcatcg atggcgcgat gaccgttgaa ttttgattcg gcaaatacag
    44761 tgatgccaac aagttctgat tcgaattctt caatcaagct cttcattccg ttgactgttc
    44821 cgccgccttt catgaaatca tccactacaa ggacacgtga accacgtttc aaacttctct
    44881 tagacagttc catcttttca attctttcag aagatccaga cacatagttg acacttactg
    44941 ttgatccttc tgttattttt gagtcccggc gtacaataac gaatggcgca ttcagataat
    45001 aggacacagc ttgggcaata ggtacgcctt ttgtggctac agtcattaca gcatcgattt
    45061 tttcaccaag gtatttagaa gcaatgattc ttccaacttg acgcagcagt tcaggttctc
    45121 ctaacaaatc agagagataa acatatcctc ctggaagcag acggtcttgt tctgatagac
    45181 gttctgctag agattcaata tattttttcg cctcttcata tgaaatttca gggatgaaca
    45241 atacaccacc tgcagcaccc gggattgttt ccaaggttcc ataccctcgt tctttaaatg
    45301 ttttcttgat gattcccaaa tcttcactga tagaagattt agcagattca taacgttcgg
    45361 caaagcttgt taaggaaatc aattcatgcg ggtgattcaa taaatactgt gtcatgtcaa
    45421 ctagacgttc actgcgacgt actttcacct attccaactc cttttaagaa attttatcgt
    45481 tttacattat ataagttttt cttcaaaaat tcgagcattt tatgaaaaaa taagaaaata
    45541 gttcggtatt tattaaaata aaaagaagcc tataaaaaat ttttcgtttt tataaggctt
    45601 cttttcttta taatttagtt attttttctt ctgaaggcaa ccaatattcg tttgatgata
    45661 tttacaatca ggaacaaagc aataaagact aatgtgattg ttgctcctgg aggtgtatct
    45721 agataaaacg agctagttag tcctgacagc atccctagaa atcctacaca aacactgata
    45781 gcaatcacga cattgaagct tttgcctaat cgcataccga ttgctgcggg aagaaccatg
    45841 attgcggata tcaaaagtgc accggcaatc gggatcatca cagcaatagc cacaccagta
    45901 atcacattga acatcatcga catcaaatga acgggtagcc catccacgtg ggcggtatct
    45961 tcatcaaaag tcagaacata catcggccgt ttgaataaaa agaacaatat accgatggca
    46021 acgaataaga ttcctaaaaa gatgacttgc tcccatgtaa tggtcacgat cgaaccaaac
    46081 agataggatt ggatacttgt tgctgaattc cctccgctca aattcatcaa tactaacgct
    46141 agcgccaaac caccagccat taaaatggca atggagattt ctgaataggt tcggtagatc
    46201 gttcgcagat actccaatat cacagctgct aatacaacga ctagcatggt cgtaagtgta
    46261 ggattaaggt tcaaaaagaa tcctaacgct acccctgcta aagagacatg agaaagcgta
    46321 tcagccatca gcgattgtct tctgatcact aaaaacacac ctagcatcgg ggcgattccg
    46381 gcaatgaaga tagcggcaag aaacgctctt ctcatgaatt catatgaaag caacgccatt
    46441 gagaatcctc ctttctcacc agtcggatct gtctgtccgc atactgtttg atatcttcat
    46501 gatcatgggt gatcattaga actgatttcc catggacatg cgcactatgt cggagaagac
    46561 gataaaattc atttctggat tcttcatcca tacctgttgt tggttcatca agaacaaata
    46621 agtcagggtc agtagcaaaa atacgcgcta aactgattcg ctgcttttgt ccgccagaca
    46681 attcacctat tcgccgattt cgcatatccc acatccctac agcttctaaa gcacgctgta
    46741 catgttgatg atctttttca gagagacgct tgaaccagcg gcctcttgga tatcttcctg
    46801 aacggactaa ttcgatcacc gtactaggaa aacctgcatt gaaagatgca acttgttgag
    46861 gaatataacc gatacttaat ttttttcctt cttgattttt ctcagcaatc tttatcattc
    46921 cttttgaagg tttcagtaat cctaacgatg ctttcaccaa ggttgacttg gctgctccat
    46981 tttctccagt taaaatgaca aattcacccg catctacatg ataagagaca tcttctagta
    47041 caggttcatc atcatagtaa aaggtcaaat cagatatttc gatataacgc accttacaac
    47101 ctccattcta aaacttaatt gatactttct tttaaagctt tcagattttt ttccatgaca
    47161 gaaatataat tttcgcctgc tttgatttgt tcattagtca agctttctaa aggattcaac
    47221 acttctagct tcactcctgt ttcattcgac aatgtctcag ctaccttcga agaggcattt
    47281 tcttcaaaat aaatcacttt gatgttgttt ttcttcacaa attcttttag ctcagctaag
    47341 cgacttggtg tgggttcctg atctggtgaa acgcccgaaa tcgctacttg ttttaagtcg
    47401 tattgattcg ctaaataagc aaaagcagca tgctgagtga caaatgtgcg gtttgttgca
    47461 tcttttagct catcggagta tttttgatcc agtgcttcaa gctttttgat ataagcatcg
    47521 gcattcttcg taaaagtttc ctttttatct ggatattttt cgcctaattc ttcagcaatc
    47581 gttctgactt cttttatagc taataccgga tcgagccaaa catgtggatc gtattcatgt
    47641 gaatgaccat gcccttcttc tgattcttca tgttcgtctg cttcttgcga ttctaaacgg
    47701 tcgattcctt tagctgcttc gatcatcaag gtctgtttgc tatccactga atcctttaac
    47761 gaatccacaa aaaattccat gtctgaacta ttatacacga aaacatctgc atcggaaatt
    47821 tctgctcttt cttttgcact tggttcgaaa tcatgcggct ccgttccagc agggatcaaa
    47881 agtttcacgt ttccttcatc ccctacgatc tcttttgtaa aatcatacat cggataaaaa
    47941 gtcgtaacga tcgtcaaatc ctcttttttg tctgcttcct tgttcgtatt tccacatgct
    48001 gcaaacagta aagcacctat cattaccgta gctgtcaaaa gatatttttt catttatatg
    48061 ctcctttcaa tgaacaaatc ggaattttac gatttattaa cgtgaactaa tgtatcaaaa
    48121 taaaaaaaca cagtcaatag aaatccatca atttctgatt taagcatgta tattcttgat
    48181 taaatcgtaa aatggatgag ttgctgctca tatctagcca tttcactaca ataaaaaact
    48241 gaagaatacc attttaggat tcctcagttt ttcatcacct attttacttc ttatatttta
    48301 ttgcagggtt cgtactaaat aaacttcctc acaaaaacct tttaacccat tatatactct
    48361 ctgcgctctt gaataatgtt gacacaaggc aaaaacggtt ggtccgcttc cactcatcaa
    48421 tgcagcatct gctccgtact tgatcatacg ctctttgatt tgatggatga ctggatgccg
    48481 ctttcccgtt atatcttcta aactgtttcc catttttttg atcatctgct gataatcttg
    48541 ctctcggatg gcttcagcaa gtccgtggat gtcttgatgt tggatggctt gcgtatctac
    48601 ttctctaaac actgtacgtg tagagacact cattcttggt ttgaccaaca caacccagca
    48661 ctgtggcata gaaggtaata cttggatttc ttcccctttg ccaccgacaa atgccgtact
    48721 tccgtagaca cagtaaggaa catctgtacc gacttccaaa ccgatttctg ccagttcttc
    48781 taacgaacag cccagattcc ataattggtt gatccctctt agagcagctg cacagtctgt
    48841 acttcctccg cctaatccag ctgctactgg aatattcttc ttaacaaaga tcttgacacc
    48901 ggttttgata ttgtatttat tttttagcaa taaagcagct tgataaacat tgttccgttc
    48961 atctaccggt aaaaatgctt tatttgtttc tacaatgatt ttgtcttctg ttatttcttc
    49021 aaaaaataga tgatctgcca agtctacgct cgacatcacc atttccaact catgataccc
    49081 atcttgccgt ctatataata catccagtcc taaatttatc ttggctggcg ctttttcaat
    49141 caattccatc ctctcaccca ttcgaaaagt ctaagtcgca agtattctat catagggaaa
    49201 aataaaagta tagcttatgt ctgcctagtg gttgattttg tcatcgtttc aacagctttc
    49261 acagaatttt ctcttcataa tccaaacaag ttctagtgat tcttataatt ctgtgagcac
    49321 ttcttctgcc aaactcggaa catttccttt ttcttttcct atcagactga gtagctgcat
    49381 ggtagcaaat ccgaaattac ttcctttcct ataagcaaaa taacgtcctt cccaatgatc
    49441 gacatatttt aatttctttt cataagcaga ctggaaggaa tagaaaaaat taccataatt
    49501 atatacgata tgcatgactt tttcttgaaa gaaagaaaaa tctgtttctc caacattagc
    49561 taatggagct aagcctagat tggcttccga gtagcccatt ttctcgtata caataaacag
    49621 attggctaac agatagtcag ctaattcctc agattcttta ggtaagaccc gcaacaaatc
    49681 atatcccgcc tgcttcttcg tcatcggctg agcggtgatg aagccaacga ctttttgatc
    49741 tttacggaca ataccgacac cgctagataa tacatatgat ttatcaaatc gtcccacaga
    49801 gaagtatttt tctcgtgcac cttgaagcca tttttgtgaa acttcattta cagcttgatg
    49861 taattcttcc ggcagttgat cgtaccaagt aaaatggtat ccttctttca tcaaacgttg
    49921 atattcaagt tgctgactca gccgagacaa gtcttctgtt gctagatcaa caatccctgc
    49981 ttcacctact ttcgtgaagt gaaaacccaa ttcatgaagc atcactacgt agctttctga
    50041 tatcttataa aacgcaagct gatagcctaa acaatctgct tgtttcataa actgattcgt
    50101 tgctgcccgc atcattttag gattgccaac tggatctcct aaaacaaagc atttgtttcc
    50161 tttgatctga taaccaaaaa cgacttgatc ttggtcgtct tcttggtaat aaaaataact
    50221 gtaacctgac aagcgaagct gatggcttgt agctgtgcct ccgtatgttt ccaataaacg
    50281 gtcaaaacgg tctttttcgt actcactgcc aagcttcgta gtatcttcag ctaaatattg
    50341 ataaagcgcc actaaagcta gcaatgagat gcccaaacca ataagccctg agaaccacac
    50401 atcatctgaa ggaaacaaaa taaaatgatt tcctgttatt cgaccgacta aaaagcgtcc
    50461 gctgtgatag cctgcaattg cgtaaacgat aaacaaaaag ccgaacaagc tagcatcaaa
    50521 gattatagct ccccaagaat aaacgaattt tgttcgataa aattcttttc tggataacca
    50581 cacagctaac agaatcaaga catataccaa tatcaactgc caagataacg tacgtgtgat
    50641 tgtatttaaa ataccaaaag atagtaaagc aatcgtaggc agaaatgcct ttttgacttt
    50701 tactgaaagt gctctagcaa ggccgagtaa taaaaagcca accaatagat tcactgtctg
    50761 atctaaaaaa ttgaatgaga acggcaataa tacttgaaat agccgactga ttgcagacag
    50821 attagtgatt gttgatagaa gtaccatcat gatccccgca aaatagacag cacttacgag
    50881 cacgatatgc gcaacctttt ggaaaaacag tcgcggaatg ttatctaaaa atcgattgat
    50941 tttgactcct gtatgtgtga tgaacaatag aagtcccgtc ataaaaggta gaacataata
    51001 aaacaaccga taatataata gccaaactac tgctacatct tgccgaacag acaattgact
    51061 cattcctaag atcatcaaaa cgtcaaaagt ccccattcca cctgggacca tcgtcagcat
    51121 tcctatcaat gtagcaatca tgaacatagg atagatcgaa agcatcgaaa tatctattct
    51181 catcaacttg ccgatgatca aaaataccgc aagggctccc gtccattgtc caaaggatgc
    51241 cagtagcaga ccaagcaagc cttttggata aaactcgaag aacaatgtct ttctctttag
    51301 ataagcaaaa aagaaaatga ctggtgctaa aaaactaccg ccgaaaagcc agatccaata
    51361 gcttcgaaaa atactttctg actgaataaa aaagacatca ataaatgtaa gaaacgacca
    51421 tacagacaaa ccagtcagca taaatagtgc gacttttgat acagtggcta aaaccatttt
    51481 tctatttgtc tgctttccat aaaaactagc ccgcagactt gctcctacca caccaccaaa
    51541 accggcgaga ttgttgatcg tgtttgttac ccacgcagca agaagaaact cttttctttc
    51601 catttttggt tttccttgtt tttctaagac tctgatcgtc acccaatcat acaacaacat
    51661 cggagtgaca cccagcagtc cagccagaat catgagcagg atcgtcatgc ggctttgttg
    51721 tcccatcctt tgccaaatat cttgccaact catgccatta gcaatatttg cgacttgatt
    51781 tgccacaaag actaacacgg agccgaaaaa aatcagtttt aaaatcagac tatgttcctt
    51841 tatccattgc agcatcaagt atcgtttatt tttcacattt tcccattcct aacttgtata
    51901 cttttgtgtc ataccccaat gttatcttat ttattttaat ttttccactt tagacctgtt
    51961 ctttgcttgc gatgatagaa aaagaaagat atcctaatgc taaaggagag gataacatga
    52021 aagtaggatt tatcggcaca gggaatatgg cacaagctat tattgatggt ttattaaaaa
    52081 aaggaacagt ctcgaaagaa gaggtgttcg tttccagtgg acattttgaa aatgcacaaa
    52141 aatttgctgc tgaaactggc gtccatgcat gcaaaagtaa tcaggaagtt gcatcacaat
    52201 cagatatcgt tcttttagcc gtgaaaccaa aaatcattga aaaagtattg gaagaactaa
    52261 gagaatatag ccagaaagtc ttattcattt ctattgcttc aggaaaatca ttaatagatt
    52321 tagaaacagc gcttgaagat cctcaggcct ccatcgtacg tgtgatgccg aatgtcaatg
    52381 tcagcgtagg agcaggcgtt tctgcgattt gcaaaaatga agctgccacg gaggaacagc
    52441 ttcagcaagc tacgatgatt tttgaagcaa tcggcagcgt ctatccttta gcagaatcag
    52501 atttcagtac attcattggc cttgctggca gctcacccgc attcgtttac atgctgatcg
    52561 atgcgatggg acggactggt gtactgaatg gtttgtccaa acagacagcc actgagattg
    52621 ctgcaaaagc aattatgggt tcttgtcaaa aattcctttc aagtgataaa aatccatggg
    52681 aacttgtaga ccaagtttct tctcctggcg gtacaactgt tgcaggtgta gttgccttag
    52741 aagaagcagg attcatccca gcaatcatca aaggaatcaa tacgacgatc gagaaagatc
    52801 ttgagatgtt gaataaaaaa taatgatagc aatgtttttt cgtgccttac aaaaaaagaa
    52861 gagctgtggc ttttgccgcg gctcttcttt tgtatttttt attatttagc ataatcagtt
    52921 gcacgagttt cccgaataac gattactttg atgtgtccag gataatccag ttcttcttcg
    52981 attcgtttac gaatatcacg tactaaacga acagcgtcta aatccgagat ttcttctggc
    53041 ttgaccatta cccgaacttc gcgtccagct tgaactgcgt aacttgattc tacaccggcg
    53101 aagctgttcg aaatattttc cagacttctt aaacgacgga tgtagttttc taatgattca
    53161 cttcgcgcac caggtcttgc tgctgagagc gcatctgctg cagctacaag gacagagata
    53221 acagaagtag cttccacatc tccatgatga gaagcgatcg cgttgattac tactgggttt
    53281 tctttgtact tggcagctaa ttccgcaccg atctcaacgt gagatccttc aatctcatgg
    53341 tcaagtgctt tcccaatatc atggagcaac ccggcacgtt ttgctaattg gatatcttcg
    53401 ccaagctctg ctgctaagat accagctaat tttgcaacct cgaccgaatg cttcaagacg
    53461 ttttgtccat aactagttct gaatcgcaga cgtccgatga tcttgattag atctggatga
    53521 agtgtatgag caccgacttc aaacgctgcc tcttccccgt attcgcgaat acgttcatcc
    53581 atttctttac gagatttctc gaccatttct tcgatacgcg ctggatggat tcgtccatct
    53641 tgtatcagtt tttccaaggt catccgtgcg atttctcttc ggattggatc gaatccggaa
    53701 agaacaactg cttccggtgt atcatcgatg attaaatcga taccagtcag cgtctcaagc
    53761 gttcgaatat tacgaccttc tcgtccaatg atacgacctt tcatttcatc attcggaagg
    53821 gtaacgactg aaacagtcgt ttctgaaact tgatctgctg cgcagcgttg aatcgctaat
    53881 gataataagt ttttcgcttt gcgatctgct tcttctttgg cacgctgttc agattctttt
    53941 accattagag ttaactcatg attcagctct tcttcagtcg atttcatgat gacatctcgt
    54001 gcgtcctctt ttgataaacc agcaattttt tctagttctt tttgttgatc ttcaatcatt
    54061 ttttcaacgt ctttttctcg ctcttcaatt aattgctgtt ttgaactaag tgcttcttct
    54121 ttactttcaa gtgagttctc gcgtttttct aacgagtcat ctttacgatt cagcgtttgt
    54181 tcgcgttgaa tcaaacgatt ctcttgagat tttaattcta acttactttc tctcagctca
    54241 ctttcaactt cagaacggta tttctgattt tcttccttag cttctaacaa tgcttctttt
    54301 tttagagttt ctgcttcttt ttttgcacta tcgatgattc cagcagctga tgattgtgca
    54361 ccagctattt ctttctcgtg acgtgacttt gcgaccataa accctaaacc aagaccgaca
    54421 attaaaccga tgatagcgag gagaatgttg aataccataa aatccacctc catactatct
    54481 ttttcatttt tatcatctat acagcttcta tgataaatta ttctaaactc gtcaatatgc
    54541 atcttcgcat atatgtgtac aaaaaacatg cacaacttta ttctaatgtt tgtaccccgt
    54601 agtgtcaact ttaaataaac aaagaaacgg ctttctactt attcattaaa cgatggtttt
    54661 taaaataaat gacactagtt tcatataggt aaacctttaa attgagaaac ttttctatta
    54721 aattattctt tgtagatgaa cagatatttt tattttatgt acccactcgt ataattaaat
    54781 aactcacgat ttctttattc aataaaaaaa cggcaggtca aggagagtac ctactcttca
    54841 gaccagccgt ttttgtttat cataattttt attcttctac taaaggcaat tcttcttgtc
    54901 ctttttcatc ttctgggaca gcgacttctt caccaatacc ataagctgct ctaactagag
    54961 cagaaacttc tgccatcatt tcaggatgat tagccatata gattttagcg ttttcccgtc
    55021 cttgaccaat ccgatcttct ttataagaat accaagcacc gcttttatca acaatgtctt
    55081 tttctactgc catgtctaga agttctcctt cttgtgagat ccctaaacca tacatgacat
    55141 ctacttcagc gattttgaaa ggaggtgcta ctttgttttt tacgactttg atctttgtac
    55201 ggttaccaac aatatctgta ccttgtttca attgttctgc acgacgtact tccaaacgga
    55261 tcgttgcgta gaatttcaat gcgcgtcctc caggagtgat ttctggattc ccaaacatca
    55321 cgcctacttt ttcacggatt tggttgatga aaatagcgat tgtttttgtt ttgttgatcg
    55381 aaccagagag tttacgcaat gcttgagaca tcaaacgtgc ttgtaacccg acatgtgagt
    55441 cacccatttc gccgtcgatt tctgctcgtg gaactaaagc agcaactgaa tcgactacta
    55501 cgatatctac agccccactt gatactaaag catcagcgat ctctagacct tgttctcctg
    55561 tgtcaggctg tgaaagaagt agttcatcga tattcacacc taatttttgt gcatattgcg
    55621 gatctaacgc atgctcagca tcaatgaaag cggccgttcc gccatttttt tgtacttctg
    55681 caatagcgtg tagtgcaact gttgttttac ctgaactttc aggtccatat acttcgatga
    55741 tacgtccacg ggggtaaccg ccgacaccta aagcgacatc taacgcaaga gagccacttg
    55801 gaattgtaga gatttgttgg tcgatttttt cgcccaattt cataatagag cctttaccat
    55861 agctcttttc aatttttttc agtgcagcat ctaaggctgc tttacgatca tctgccaata
    55921 cgataatcct ccttatattt acaaatcaga gcaacatttc attatcctta tcatatcttt
    55981 tttagtgaaa aaaagcaaga aaaaaccgaa caaatattcg catttatcat tttcttaaaa
    56041 gctgttttct caaaagatca aatccttgca taaccgcatt atatctgata gcagcacgat
    56101 ctctggcaaa gtgatatttt tccgaaacca cgccgtcttt agatgaaata gcgatccata
    56161 ctgttcctgc agattgacct tctaacgtgt ccggtccagc cactccggtg aaagctacac
    56221 tgtaatctgt tcttgctatc tcacgtgctt gaatggccat ctgctccaca cattctttgc
    56281 tgactgttcc atattctgct aatagctccg gatcaatctc cagaaaacca gcttttgttt
    56341 ggagcgagta ggtaacgaat ccgcctggaa atacttcaga tacaccagca atgtcgccta
    56401 atgcggcttg gaaggcgccg gctgtcaaac tttccgctgc tgttacagtc tgcttgtttt
    56461 ctttcagtag ttccactacg acttttgcta gcgaattatc atcgccgtat ccataaaaat
    56521 attcccctac tcgttcttgg attttttctt ctaatgctaa aagagcttga ttccctgcct
    56581 gaacatcatt tgttttcact gtcagtcgta atgtcacttc atttggtttg gcatatggtg
    56641 cgatcgttgg attgatctgt gtctcaatca gatcttttag ctcagtgacc agtcgcgatt
    56701 caccaatccc ataaaagcgc aaaactttag aaattagttt ttcttcagaa ggaaaacgtt
    56761 cctctagtaa aggacgtact tgttccacaa acattggttt caactcatta ggcggaccgg
    56821 gaagaaggat atacgcatgt gtatctgttt gataaaaagt ccctaaagca agccctgtac
    56881 gatttggtag aggaaccccg ccttcgataa tctgggactg ttgcagattg tttttggtca
    56941 tttttcgatg ggttgtttca aaatatgcca ataatttttt gtatccttcc gtattttgta
    57001 tgagagactt accgaggtga gccgcagttg cttcttttgt caggtcatct tctgtcggtc
    57061 ctaatccgcc acataagagt atgagttcac ttcttgtttc agctagagca atcaactctt
    57121 ctaaacgctg ctgattatct cctacaactg tttgataata aacttcgata cctagatcag
    57181 ctagctgttc tgacaagaaa gtggcattcg tattcacgac ttgtcctagt aaaagttctg
    57241 ttccgacagc tattatttct gctttcatta cttcccgctc ccttatatag tatacgctgt
    57301 atttttatat ttttttattt aaaaagaggg caaggagctg tgacaaaaat cttgtcacag
    57361 ctccttgttc caaataaaca gtgttctcct gccaaaacct cggtcataag tcacaatata
    57421 cataaaacat gaaaagctgt tttttgaaat tatcccttgt gactcagagt tagacggcag
    57481 gctcacaacc tctccactaa acgatactta cattgatcct ttgaacacac cgctgttttt
    57541 gatgaagtaa tccacacctg aatagatagt aaagatcaga caagcataaa gcatgatttg
    57601 atctactgga ataccgatca ggctgaatgg gatattattg ataaacagca agataatagc
    57661 aaccatttgt gtagccgtct tgacttttcc cggccaagct gcagccatga cttctccatg
    57721 ttcgacaagt agtaaacgca agcctgtgac ggctaattca cgacagacaa tgatcgctac
    57781 tacccaacca ggggcctttc cttggtcaac caacatgata aaagcagtca taactagcat
    57841 tttatctgcc aacgggtccg caaacttgcc aaaattagtg acaaggcctc gcgcacgggc
    57901 aatttttcca tctaaccagt ctgtgatact agctaaagca aaaataactg cacctactag
    57961 ctgtgtgaca gccagtgtgg tgtctcctac gttaaattgt ccccaatcta aaggtaaacc
    58021 tacaaccaaa atgaaaatcg ggatcataca aattctaagt acagttaatt tatttggtaa
    58081 attcacacta tcgactcctt tcttccctat tctacgtgac tatcagcgaa gtttccaccg
    58141 atattattta cttttccaaa tgaatttatt caaaggagta tttttattga aattgatctt
    58201 tttgaattaa cgaagaagga ccgcgacaca gatttgtcac gatccttctt ttttatacct
    58261 acaagtaaac agatgcgtaa actaatttat acgatcattc tgcgtattga agagtaaggt
    58321 tcacattttt tggactctga ttgttttccc ctggattaac tggtacggat tctccattcg
    58381 ctttgatcgt tgcattgctt gctgcaccaa tcgtgatggt cgcatttgtt gctgtttccg
    58441 gtaacacggt actctgtgtt tcatttgctg ccagtgtccc ttgataaaca taggcattgt
    58501 ctactagcac acctaaccat acacgattga cagcagtaaa ttcaagggtt acaggcttgg
    58561 tagcgtctgt gatatttact gcgatggcac tgccagaatc ttctcctgta gctattgcca
    58621 ttttcttttc ttcacttgat tttgttgttg attctactgt agaagactct ttggtctgcg
    58681 tgctttgctc tgaggaagag gactcctcga ctgatccttc tactttaagc gaagaagctg
    58741 tgtctccgat tattggatca gcttgacgat cttgccaagt catatagccc acgacaacga
    58801 caatcgccaa agcaaccaat cctaataaaa tgacaggaag acttgtccaa aagcgacttg
    58861 gatgcgattc ttcctgatgc attgcttttc tagaagtact tactgtttca ggctgcggac
    58921 gttcagggac cgatcctgtc aatttgcttt tcccatcaaa aacatcgact aaatggtctc
    58981 catcttctcc tacagcttca gcgtattgcc gaataaatgc ccgcacgtaa aatttaccag
    59041 gcaattggtc aaaatcatca ttgtcgattg cttccaaata acgtttttga atttttgtaa
    59101 tctgctgtaa ttcatctaaa gatatttttt tattcaggcg tgcttgccgt aatttttctc
    59161 cgatggtttc aaaagccact cgttcatctt ctccagtctg tttgattctc cgaagcatct
    59221 gctgttgcaa cgaaagacgt tatgtttggg tgctgtgaca aaagtcatgt cacagctcct
    59281 tttttccgaa taaacattgt tctcctgcca actcctcggt cacaagtcac aatatccaat
    59341 aaatataaga agctgttttt tgaacttgtt ccttgtgact cggagttaaa cggcaggctc
    59401 acgacctctt tcttaaacgg tgttcaatca gctgttcctc ggtattagct caaaaaccgg
    59461 caaaatataa ggagatattt ttccgtatgt ttctctaata ctcaggtcaa aacgctgatt
    59521 tcacaacctc ttccttaaac ggtgttcaaa agcttgcatc tgcggtaata agtccaacca
    59581 agttaaaaac attaagaagc tgtttttact tggctgttct tattacttga tgcagggcag
    59641 ctttttcaca acctcttggt acacggtgtt ccagaagtag cttcttcggc aataagccga
    59701 aatatcacaa aaatttgaga agcaattttc gtaaattcct tcttatttct tgaagctgac
    59761 cacttctgtc acaacctctt tgattacttc gtttgatttt attttaatat aagttgatta
    59821 aaagagagca tgatttgttt tattttctca ctaatttttc caacacaact aagaatacca
    59881 taaaagctga ttttggagga ggttcctact acaagtctaa aacgaaattt aagacttttc
    59941 ctcataaaat actggtttat tttggtctca tgtaaaactc gctgaaggcc tcttcactta
    60001 taaatgcttc cccagctgcc agtacgtctt tcatttggat cgaatcgatg atttcaggaa
    60061 gatcaaataa agttcgatcg ccaaataaac tttgcgtaaa ttgattggca atatattcga
    60121 tcgaatttaa tgattggaaa tattgtccaa gcattttctt cttcagcaaa tcgagatttg
    60181 tttctgacac ttccggatca tcagcgaatc caagaatgat ttctttgact ttttctgctg
    60241 ctttctctgg ttcgtccgta tctccgctga agtccgcaaa gtgaaattca cgatccaagc
    60301 tgaattcaaa accaaagctg tcatcgataa ttccttgatt gtacatcgct agatagtttc
    60361 gagaagtatt gcctaaaatc atttggaaca acagattgat tgctgtttta tagcgtaaca
    60421 gttctgttcc ttcttgcggc aatgtgtcta atcctttaat accaagtaca aatttatctc
    60481 gtgtaatggc tgcttccaat gcactttgtt tgatgatttc ttttgtgttt tcagggaaat
    60541 aacgtacaat ttcttgcttt ggagggaaat tttttgcttc ttggttttcc cgaataagtt
    60601 tcatcaattt ctcaggctcc atcttaccaa ctacaaataa aaccatattg cttggctgat
    60661 aaaacgtccg atagcacgta tacaagtctt gagctgtgat tttgtcgata ctttcaaccg
    60721 tgcccgcaat atcgatatgc aatgggtgtg taggatacaa gttatttagt atgccaaaaa
    60781 acatccgcca gttagggtcg tcttcataca tttggatttc ctgaccgatg attccttttt
    60841 ccttgtttac agtttcttct gtgaagtaag gtgcttgtac aaaatcgatc agagtcgtca
    60901 gattcttttc tacctgatcc gtcgtagaaa acagatagct ggttttcgta aaacttgtga
    60961 aagcattggc ggaagctcct tgtttcccaa ataattgaaa gacatcacca tcttcttttt
    61021 caaacaactt atgttctaaa aaatgggcaa ttccatctgg aactttcacc ttttcttttt
    61081 caccataggg aataaattcg ttatcgatcg aaccataatt cgtactgaac aaaccatatg
    61141 tcttgtgata gtcattttta ggcaacaaat acacggtcaa accgtttggc agtacttcat
    61201 gataaagtgt ttcattgatt tgctcatatt ctgttttatt catttgcatt tcctcctgct
    61261 aaaaagaaga ttgcctgcaa ttcgatttgc tcagctactt gttgtatctc tttgatcgtt
    61321 acgctctcca tacgtttcaa ccattcttca tcagataatt ttgtttccgg cagccagctg
    61381 tccaaaaaag aagcttcgat tgccgcttgc ggagagtcta gagaaagcag atattggttt
    61441 ctcagcattg cttttgtctg agacagttcc aactctgtta tttcaccatt tcgcaaactt
    61501 tctaattgct cgtgaatcag atgaagtact tgatttcggt tcttttcatc gataccggtt
    61561 tggacactca tgaatcctcg gaacgtatcc acgctactag aggcataata agctagactt
    61621 tctttctctc tgacattcat gaaaagttta gagtgaggaa aaccgccaaa taaaccgtta
    61681 aagaccatta gcgcaaaacg ttctggttca tcgtaataca catttgtctg ataagctagg
    61741 ttcagctttg cttgaacgat tggctcacgt acttgtcgtt ccttcacgat attcacttga
    61801 ggttgggtat aaaacagatc tggataaact gtttcccgcg actcaaatgg taattgccca
    61861 actgcttcag ctgctttgtc cggatcaaca tcgccgacaa caaaaatgtc gatttgatca
    61921 tttgccatca tttcttgata gtaggacgct agcgaactag cagtgatctt gtccaaatct
    61981 tcgactacgc ccaaccctgg gatcttttgt tccgggctgt tttcaaaata aagttcttgc
    62041 aaggcatagg aagcaaaagt ttgtttatct tccttcaaac tttccaaata tagacgcaga
    62101 ttgttctttt ccaagtcaaa cgtcaactga tcaaactgat gatttttaat atttggataa
    62161 aataaaacct cttttaaaaa atctaccgct tgagaaaaaa ggtttggatc attgacatat
    62221 tcaccattca caaaactgat ccctgcgttt agccaatgta aatttccttt ttttctaact
    62281 gataatccaa agcttgcgcc ataaagctct gcaagttgcg cacttaactt tgtctgatcc
    62341 gaataattca aactatttgt ttccaacatg ctgctgagaa gtgaacgttt cgtgatgact
    62401 tcttgttgca gacgcgcagt aaaacggata aaaatccgga tcgttttgta ttgttttgtt
    62461 ggtaagacat gaagattcac gccttctgcc aattgataca ccatatttat atgctccttt
    62521 tcacagttga ttgcccgctt actcttttaa tgggtcaacc tgcctttttt acttattcct
    62581 ctgcttgaaa tttataagtt caggcaattc gccgtttttt ctaaatgatg ttacacttta
    62641 tagtatagca tagatgtttt ttatcaaaag aaacagacgg ctaaaagcgt atattgtttt
    62701 tttatttttt atttttcgat atactaaaga caaataaaag aagggagggt cattcatgat
    62761 caaagaattc aaagagttca tcatgcgtgg agatgtcttg gatcttgctg tcggcgttgt
    62821 tattggtagt gcgtttacaa gcatcgtaaa ccaagtggtg caaggcttga tcacaccact
    62881 aataggttgg tttgtcgcaa tgatcacagg tacttctgat ttagaaggag cactttcaat
    62941 tcttgactgg tcgccgacaa aaggcgtcac atttgcgttt ggtaacgtta ttagtgctat
    63001 cattacattt ctgatcactg gttttgtctt attcttagtt gtcaaagcgg caaaccgtgc
    63061 gaagaattta cgctccaaag aagagcaagt ggaagtggaa gaaactccta ctgcagaaga
    63121 ttatcttagt gacatacgcg atcttcttgc caaacaagtg gcagaagaca atacacatcc
    63181 agtagagcaa acaaatcttt ccgatacttc taaataataa agaatctaaa aaaaaagaaa
    63241 gccgattttc agtgtcggct ttcttttttt agattcttaa cttgctactt gttccttttt
    63301 catgtcgata tgtggaatat catcttccaa atacacatct gagattgcct tgaatccaaa
    63361 cgactcataa aaagcttgta ggtattcttg tgcgccgatc acgattggtt tttctggaaa
    63421 atttttctgg atcatgttaa tcgtttcttg aacaatcttt cttcctaaac catctttacg
    63481 gttgtcctga tgaacaagga cacgtccaaa atggatactc tccgcttctt catatactcg
    63541 cgtgtaggca aggatcttcc catcagtatc ttgaaaaaac gtatgccaag cttctccatc
    63601 aatgtcatca acttcttgat aaggacactg ttgttccaca acaaaaactg cgattcgttc
    63661 tttgacgatc gcgtaaaact ctgctgttga caattcttca aatttcttta cctgatattc
    63721 cactaagcaa acgccccctg cttttttatt cttagcatac cgattcgcac acaaaaaagc
    63781 aaacgaacct tacgaatctc aggctcgttt gctttttaaa ctgcttcagt taaaaattcg
    63841 atctcaactg tccgagtaag tacatctgaa tagctgtaag atactcgttc aaaagaattt
    63901 tcttcaggat ccaaatcaac aacgaatact gaaggatagg tttctgttaa aacgccgcgg
    63961 cgttctgttt gacgctttct tcctgtctga gcaaccaaca tgattgggct gccgatacga
    64021 ccttctaaat ctttcttgat tgaagctaaa gttgttggca tacagtt
//
LOCUS       DAYYCY010000003        49741 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000003 DAYYCY010000000
VERSION     DAYYCY010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49741
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.003"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1204
                     /locus_tag="ACHAZB_000136"
     CDS             <1..1204
                     /locus_tag="ACHAZB_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFM8612852.1"
                     /translation="AADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQAIINNV
                     DFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERYWFSFPK
                     NVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYHIGLYHG
                     EPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGHTKKEQA
                     STSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSKVEDGFS
                     LIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTFYTKTPL
                     AASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAKQLLNQD
                     FLFEEDQE"
     gene            1201..3921
                     /locus_tag="ACHAZB_000137"
     CDS             1201..3921
                     /locus_tag="ACHAZB_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8612853.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            3905..4849
                     /locus_tag="ACHAZB_000138"
     CDS             3905..4849
                     /locus_tag="ACHAZB_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFM8612854.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(4918..5927)
                     /locus_tag="ACHAZB_000139"
                     /pseudo
     CDS             complement(4918..5927)
                     /locus_tag="ACHAZB_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
     gene            complement(6107..6451)
                     /locus_tag="ACHAZB_000140"
     CDS             complement(6107..6451)
                     /locus_tag="ACHAZB_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFM8612855.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(6444..6878)
                     /locus_tag="ACHAZB_000141"
     CDS             complement(6444..6878)
                     /locus_tag="ACHAZB_000141"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HFM8612856.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            7016..7753
                     /locus_tag="ACHAZB_000142"
     CDS             7016..7753
                     /locus_tag="ACHAZB_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8612857.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            7746..8957
                     /locus_tag="ACHAZB_000143"
     CDS             7746..8957
                     /locus_tag="ACHAZB_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8612858.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            9161..9943
                     /locus_tag="ACHAZB_000144"
     CDS             9161..9943
                     /locus_tag="ACHAZB_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HFM8612859.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            10013..10675
                     /gene="trmB"
                     /locus_tag="ACHAZB_000145"
     CDS             10013..10675
                     /gene="trmB"
                     /locus_tag="ACHAZB_000145"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFM8612860.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(10773..12527)
                     /gene="ade"
                     /locus_tag="ACHAZB_000146"
     CDS             complement(10773..12527)
                     /gene="ade"
                     /locus_tag="ACHAZB_000146"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFM8612861.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            12659..13588
                     /gene="comGA"
                     /locus_tag="ACHAZB_000147"
     CDS             12659..13588
                     /gene="comGA"
                     /locus_tag="ACHAZB_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus ATPase ComGA"
                     /protein_id="HFM8612862.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGTVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLKLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            13545..14594
                     /gene="comGB"
                     /locus_tag="ACHAZB_000148"
     CDS             13545..14594
                     /gene="comGB"
                     /locus_tag="ACHAZB_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus assembly protein ComGB"
                     /protein_id="HFM8612863.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            14591..14869
                     /gene="comGC"
                     /locus_tag="ACHAZB_000149"
     CDS             14591..14869
                     /gene="comGC"
                     /locus_tag="ACHAZB_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus major pilin ComGC"
                     /protein_id="HFM8612864.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            14844..15314
                     /gene="comGD"
                     /locus_tag="ACHAZB_000150"
     CDS             14844..15314
                     /gene="comGD"
                     /locus_tag="ACHAZB_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGD"
                     /protein_id="HFM8612865.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            15286..15633
                     /locus_tag="ACHAZB_000151"
     CDS             15286..15633
                     /locus_tag="ACHAZB_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8612866.1"
                     /translation="MVAIQKELNKTGFVLLESLTALMIASVCVYLVSFGQLFLLQAEK
                     KSGEELLFIRMIYEDIKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAI
                     SIEKTSNSSIYAD"
     gene            15596..16021
                     /gene="comGF"
                     /locus_tag="ACHAZB_000152"
     CDS             15596..16021
                     /gene="comGF"
                     /locus_tag="ACHAZB_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGF"
                     /protein_id="HFM8612867.1"
                     /translation="MKKQVIPAFTLIECLIGLMILSSLLLTFNLLIQQTIQVSEFLQR
                     KDQKEWLIFLAQLEEELKSSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQ
                     PMLTSITELQLLEKEHTISFYVHFENGEDGYAIWTKNDQ"
     gene            15993..16352
                     /gene="comGG"
                     /locus_tag="ACHAZB_000153"
     CDS             15993..16352
                     /gene="comGG"
                     /locus_tag="ACHAZB_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGG"
                     /protein_id="HFM8612868.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            16524..17531
                     /locus_tag="ACHAZB_000154"
     CDS             16524..17531
                     /locus_tag="ACHAZB_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8612869.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            17556..18740
                     /locus_tag="ACHAZB_000155"
     CDS             17556..18740
                     /locus_tag="ACHAZB_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HFM8612870.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(19177..20760)
                     /gene="abc-f"
                     /locus_tag="ACHAZB_000156"
     CDS             complement(19177..20760)
                     /gene="abc-f"
                     /locus_tag="ACHAZB_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protection-like ABC-F family protein"
                     /protein_id="HFM8612871.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVDINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHCQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(20865..20993)
                     /locus_tag="ACHAZB_000157"
     CDS             complement(20865..20993)
                     /locus_tag="ACHAZB_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8612872.1"
                     /translation="MMRMTKITSNENYQRYHIAFSHAIGFTVAVDGTSLSKTVEFT"
     gene            complement(21338..21481)
                     /locus_tag="ACHAZB_000158"
     CDS             complement(21338..21481)
                     /locus_tag="ACHAZB_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFM8612873.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(21703..22182)
                     /locus_tag="ACHAZB_000159"
     CDS             complement(21703..22182)
                     /locus_tag="ACHAZB_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFM8612874.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(22255..22539)
                     /locus_tag="ACHAZB_000160"
     CDS             complement(22255..22539)
                     /locus_tag="ACHAZB_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8612875.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(22617..22811)
                     /gene="ssrS"
                     /locus_tag="ACHAZB_000161"
     ncRNA           complement(22617..22811)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHAZB_000161"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(22883..24169)
                     /locus_tag="ACHAZB_000162"
     CDS             complement(22883..24169)
                     /locus_tag="ACHAZB_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HFM8612876.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            24307..24978
                     /locus_tag="ACHAZB_000163"
     CDS             24307..24978
                     /locus_tag="ACHAZB_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HFM8612877.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            24944..25432
                     /locus_tag="ACHAZB_000164"
     CDS             24944..25432
                     /locus_tag="ACHAZB_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HFM8612878.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            25445..26392
                     /gene="prmA"
                     /locus_tag="ACHAZB_000165"
     CDS             25445..26392
                     /gene="prmA"
                     /locus_tag="ACHAZB_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFM8612879.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            26393..27157
                     /locus_tag="ACHAZB_000166"
     CDS             26393..27157
                     /locus_tag="ACHAZB_000166"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFM8612880.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            27390..29603
                     /locus_tag="ACHAZB_000167"
     CDS             27390..29603
                     /locus_tag="ACHAZB_000167"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFM8612881.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            29614..30060
                     /gene="dtd"
                     /locus_tag="ACHAZB_000168"
     CDS             29614..30060
                     /gene="dtd"
                     /locus_tag="ACHAZB_000168"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFM8612882.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            30231..31490
                     /locus_tag="ACHAZB_000169"
     CDS             30231..31490
                     /locus_tag="ACHAZB_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HFM8612883.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(31579..32526)
                     /locus_tag="ACHAZB_000170"
     CDS             complement(31579..32526)
                     /locus_tag="ACHAZB_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFM8612884.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            32920..34212
                     /gene="hisS"
                     /locus_tag="ACHAZB_000171"
     CDS             32920..34212
                     /gene="hisS"
                     /locus_tag="ACHAZB_000171"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFM8612885.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            34227..35996
                     /gene="aspS"
                     /locus_tag="ACHAZB_000172"
     CDS             34227..35996
                     /gene="aspS"
                     /locus_tag="ACHAZB_000172"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFM8612886.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            36105..36980
                     /locus_tag="ACHAZB_000173"
     CDS             36105..36980
                     /locus_tag="ACHAZB_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM8612887.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            37246..39129
                     /locus_tag="ACHAZB_000174"
     CDS             37246..39129
                     /locus_tag="ACHAZB_000174"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFM8612888.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(39204..39488)
                     /locus_tag="ACHAZB_000175"
     CDS             complement(39204..39488)
                     /locus_tag="ACHAZB_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFM8612889.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(39478..40212)
                     /locus_tag="ACHAZB_000176"
     CDS             complement(39478..40212)
                     /locus_tag="ACHAZB_000176"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HFM8612890.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            40341..40964
                     /locus_tag="ACHAZB_000177"
     CDS             40341..40964
                     /locus_tag="ACHAZB_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFM8612891.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            41156..42277
                     /locus_tag="ACHAZB_000178"
     CDS             41156..42277
                     /locus_tag="ACHAZB_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFM8612892.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            42284..45412
                     /locus_tag="ACHAZB_000179"
     CDS             42284..45412
                     /locus_tag="ACHAZB_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SbcC/MukB-like Walker B domain-containing
                     protein"
                     /protein_id="HFM8612893.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANERIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            45431..48625
                     /locus_tag="ACHAZB_000180"
     CDS             45431..48625
                     /locus_tag="ACHAZB_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HFM8612894.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(48771..49478)
                     /locus_tag="ACHAZB_000181"
     CDS             complement(48771..49478)
                     /locus_tag="ACHAZB_000181"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8612895.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        16201 a         8770 c        10395 g        14375 t
ORIGIN      
        1 tgcggcagat ttacatatgg atcggtcatt tgaaggactg acaactcttg ataaaactgt
       61 acaggaaaaa ctattgaaga cgaatctgac cgttttatca aatatcattg aacaggcaat
      121 aataaataac gttgattttg ttttacttgc aggagataat tttcatcaaa atcgtccttc
      181 attaaaaata cagaaacatt tttcagaaca gatgaaacgg ttagaaaaga accatatacc
      241 tgtatttata atattcggaa atcatgattt ctatcaaaaa gagcggtatt ggttctcctt
      301 tcctaaaaat gtccatttat ttacatccga aacggtagag acaaaaaaaa taacgataaa
      361 aagtggagaa actgtctctc tcagtggatt tagttatcgt caaccgtgga ttcaaaaaga
      421 caaagtgatg gaatttcctt ctagagaact cacagattac catattgggt tgtaccacgg
      481 ggaacctggc gtctctcaaa aaggaaacta tgctcctttc caaccctcaa agatgcaaga
      541 aaagggatac gattattggg ctttagggca tatccatgtg ccaacggtct taaatgaaaa
      601 gcaaacaatt gtttatccag gtgcgcctca aggtcatact aaaaaggaac aagcttctac
      661 atctattcta ctagttgaat taagtaaagg aagttgccaa atacatccga ttaaagtagc
      721 agaggtctat tggaagacaa aggaaatatc attgaggaaa gcaagaacga caaaagaggt
      781 cattgttcac attcgtcagc agctttcaaa agtagaggat ggatttagtt taattgagat
      841 aaaattaaaa gattatgacc atctcaatac agatgttttg gaacggatac aatcaggaga
      901 gctattagac tatttgttag aggaattctc agataggata aatgatttct ttatttggcg
      961 tatttctctc atagagaata ctttttatac gaagacccca cttgctgctt ctgctaaatt
     1021 gatggaacag ttatttcaat actatcagac acctcaagat ttccaacaaa ttttaaatga
     1081 agtatatagt catcaagaag cggctagaat attgagcgaa ctgccggaat atcaagaaga
     1141 aacacttgaa aaagccaagc agctgcttaa tcaagatttt ttatttgagg aggatcagga
     1201 atgaagattt taaggatcga gattgctgct tttggaaaat ggcgtcaaaa aagttttgac
     1261 ttttattcag gcaatcagct gatctatggg ggaaacgaag caggaaaatc aacgatttat
     1321 caatttattc aagcaatatt atttggtttc ccttcaaaag gccgtaaaaa aaaggattat
     1381 acaccaaaag acggctcagc ctatggcggc aaaatatggc ttaaacatcc tgtatatggt
     1441 gaatttgctg tcgagcgcta caagcagcaa aatcgcggaa aatcgaaggt gtggctgggg
     1501 gatcaagtgg gaagtgatga gttactagaa aaactgatcg cacctttgac gaaagagtta
     1561 ttccagcagg tatttacttt ccagcaagag cagttgatgg aactagatca tttgagagaa
     1621 aaagagctac atgatgcgct gatttcttta ggcatcacgg gcagttctca agtattccaa
     1681 aagcggcaag aatattatca gaaagcacaa caattattca agaaaaaagg ccaaaaatta
     1741 ctaatcaatc aaaaattaaa tgaatggaaa gagctgcaag aaaaaattca tcaaaaacaa
     1801 gaacaggaag cttattttgg caagttgatc caacaagaac aggaatacga gtcgcaacag
     1861 cagcagttat tggcacaatt aaaagaggcg aacaaacaat tttcattagc cagacagcaa
     1921 gaacttaatt tttctttata tgaagaatgg caatctctca aaaaaaacag cactacaggt
     1981 cttcctgatg agcaacttct tttagatctg gagtctttct ccaaacggta tcagcaaaca
     2041 aatgagcgtt tggaagaatt agaagcaaca ctggaaaagc atagcgggat ggatcagcag
     2101 tcagccagat attattttta tttagagcag gagcaggaac taaaagatct ccagcaactt
     2161 caatttacag cggaacaatt aaacgaagaa tggcaaagat tacaaattga gactagagaa
     2221 acaaattcag aattgaagat tttggaacaa aggtggcatt ggcagaaaaa tccgttaccg
     2281 gaagaatttt tatatgaaga agaatggaag catcttctgg ctgaagaaag aaggattgct
     2341 gatctagaaa gccagatttc tgtgcgtctc caaatagcta aagaacagca agagatgatt
     2401 gaagcagaag tgaatgactt ggaagagacc tatccagcat tattacaaaa tcagtcagag
     2461 atagcaccag tctcttcatc taaaccgtat atgctgatgg ctggcggagc aataatcatt
     2521 gcaggtatat ttgtaccaat gcccttgaaa gtgatccttt tactaattgg tgcaggcatg
     2581 attggctatt tcttctacaa aaggcaacca aaaattaaaa ctaaaaatga tttctctgag
     2641 ataaaaggaa tgtggcaaga aaaactagga caacttgatc tagccttagc tcaattagct
     2701 gaagcagaag aaaaacaaaa gagaactgga gaagaaaaac agattcttca gcaagaagta
     2761 tatcgttttg cggatcatcg gcacttagga aagatggata cgctcaaact catgaaagaa
     2821 tatggacagg aagtcataga ttatcagaaa ttatatggcg tatttgagcg gaaacataaa
     2881 agagaacagg aaatacaaga aaaactacga caaatcgacc aaaaatttga gtttgttaaa
     2941 gaatggttac cgatacaaga taagacaatt tcagaaaaaa tggtattgct ttcccgtttt
     3001 atacaagaga tggaacagat gaaatttgcc agaagctatc agcaaaatac tttattgaag
     3061 caagaaatca atcaattgaa aaagaaacaa aaagtatgga tacaagaata cagcgaacaa
     3121 ttgactcaag caggcttatc ttatccttcg gaagtacctg tctttttaca aaaagccaaa
     3181 caagccgaac agcagatgaa aagatacaat gaattatcgg aaatgcttac tccactattt
     3241 cctgaagagc tgacaaaaga gaaattatct gtaagactgc gccaactgaa agaaaagcaa
     3301 tcccaactcc agcaagaaga aaaagaagca agtgaaaatc ggcaacgact ccaacttcaa
     3361 gtagaagagt tgcaaagaga cggcacattg gacgaacttt atcaacaagc tagccggcaa
     3421 aaagcaaaat tacaggaatt actggaggaa tggggagcac ttgaaatcgc cgggacattc
     3481 ttaggtgatc tttcaacaga gatgagtgaa agacaattac cccagttgtt aagagcagca
     3541 ggtgattatt taggtatttt aacaaaagga aattatcaga agatattact gaaaaatgat
     3601 acactgactt taacagacgg atatacagaa tttcccatct atgagttatc tacaggtacg
     3661 aaagatcagc tgattatggc gatgagattt gcttacctct cattagaagc taaccgttcg
     3721 atatgcccga tcatcattga tgatggctgg cttcattatg atcatcagcg aaagtatcaa
     3781 ttagctgaac ttttagctaa atttggagaa aagtatcaaa taatttgttt ttcttctgat
     3841 aaagaaatgg taagctatta ccatgagatg aaccaaatcg taaaacaatt agaaggagca
     3901 aataatgaaa aaaattcgtg atttagtaat cgatgagcag tttgaacaat ttgtcctgat
     3961 caaaagtgca gatgtaagaa tcgctaaaaa cggaaaaaaa tttattgctt ttactttcca
     4021 agatacttca ggtactatcg atggtaaata ctgggatgct tcggaggagg aaatcaataa
     4081 atttacagcc ggcacagtcg tttttctaaa cggtaaaaga gaggtttatc aaggaaaccc
     4141 tcaagtaaag attcttcata tgcgcttaac aagggtagat gagccaggag agcctagttt
     4201 atacatggaa agagcccctt tgaagaaaga agaaatggaa gaagaaatca atcaattgtt
     4261 gtttgagatc accaatgcgc attggaatcg tattgttcgc tatctactta ataaatatca
     4321 aaaacaattt tttgagtttc cggcagcaaa aagaaaccat catgcatttg ccagtggatt
     4381 agcttatcat actttgacaa tgcttcactt agggaaatca atcgccgatg aatatcctga
     4441 gctgaatcga gcattgttgt atgctggtat cattttgcat gatttaggaa aggtgattga
     4501 actttccggt cctatgtcga cagaatatac gcttgcagga aatttgatcg gtcatctggt
     4561 attgattgac gaagaaataa ctaaagcctg tcttgcgttg aaaatcagcg aagctgacga
     4621 agatgttctt gtattacgtc atatggtttt agcacaccat ggcttattag aatatggctc
     4681 acctgtacgt ccaagaataa tggaagcaga aatattacat caaatcgaca atatcgatgc
     4741 ttcgatgcag atgatgttga cttcgattcg tcaaacagaa ccaggaaaat acaccgatcg
     4801 gattttcggt atggacaacc gaagtttcta tgtaccaaaa gatttatagc aaaaatttat
     4861 ttttttcatt caaaacaaaa aaagcgagac ataagtcttc gctttttttg ttttctttta
     4921 ttttgatgaa tcagttgttt cttcagttga agattctttt gttgaatcag ttgattttgt
     4981 gtcagaactc tttgttgaag ctgtagtttc acttgagtct ttcgtacttg atgaagttgt
     5041 tgtgaatgca ctcaacacat tttcaaaggc atcatctttg atttttacat ttgcatcttt
     5101 taattcttca ccgattactt tagttgtgaa tgcattgtca cttagtttag tttcagtagc
     5161 gatatctttt agttgatctt tgtatttatc catgtcattg cctttatttt ggtttttcac
     5221 cattttgaca acgtagtatt ctgtcgcata agaagtagga tttgttgtag taataacatc
     5281 ggagatctca ccgtctttca atttgaaagc tgcttctttt acttctgcag gaatagttgt
     5341 tgtcgttgag tcgaatttaa ctttaccgcc atcttctttt gtctctgtat cagttgattt
     5401 gtctttggct aatttagaga aatcatctcc atcatccgct gattttttca catcttttgc
     5461 ttcatcttca cttgataatt tgatgatttg tgcttctact tctggatgga aagatttcca
     5521 agcagttttc aaatcgtcat ctgtaatatc tacgtgagct ttcaagccag cttcaaaagc
     5581 taaattgtta cgaatatatt ctttataagt atctttcgta tatccagcag cttctaattg
     5641 tgattcaaaa gtatcaccta atgatttcgc ttgtttgtcg tattctgcat ccacttgctt
     5701 atcggttact ttatcgccat atttgttatt gaatacttta taaatgatca tccgttgtac
     5761 taaggattgg ttcgatgatt caagttttgc ttcatcatag aaatcagata cagtgatcgt
     5821 tccgcctttc atcgttgcga tatctttatt agtatcgcct gacaagctgc tagagttaaa
     5881 acagccaacg cactagtagc tgccaaaata attgattttt ttttcattaa aatttgcact
     5941 cctgtaatta gttattattt ttatatatta actataccat atcccatttc ctttaaaaac
     6001 gttgtcgtgg tagtttcaaa caaaattcaa aatttcatca cattcctagg aaatacgcaa
     6061 acttttctca cttatatagt taaatctgtt gagaaaataa tcattattat atttgttcag
     6121 gatactctgg cagatcagct tttagttttt ctacttgttc attgatttgt gtgatacgtg
     6181 gttcggcttg gaatttaaaa tcttcgatat cttttttgat tgcttcttgg aaagcaggaa
     6241 tcacagattc agctgtttct tttgtttcga tcaatgcgct tttaaaatga ttcaggctgt
     6301 tatttaaatc atttgtcaga gttgttgctt cttccaattc ttcgatcagt ttctttcttg
     6361 tttcattccc gctcctgggc gccaataaca gacctccgac agtgccagca gcagctccaa
     6421 ataacagtcc tttgaaaaag tttttaatca tcgcaggaca cctgctttac gattgtttct
     6481 tgaatagctt ttagttcttc cgatccataa ctatcttgat gattgacaaa gtgaatagaa
     6541 aaatcatcct ctttgctgta gcgagggact aaatggatat gtgaatgaaa aacagattga
     6601 taggctagtt cacggttgtt attcaagata ttaagacctt tcatttctgg aaaagctttt
     6661 tccaatgctt gagcaatctt aggcacacgc gaaaaaacct ctcctgcaag ttcgggatca
     6721 tattcaaaaa tatctgctac atgacgttta ggcacaacta atgtgtgtcc tttcgttgct
     6781 tgagagatgt ctaaaaaagc atagaccttg tcgtcttcat atattttata gcttgggatt
     6841 tcttgattga tgattttgca aaaaatacag ttttccatat aaaacatcct ttctataaag
     6901 aataaatact tcagcttaac tttaccataa gttaaagcta ttgtaaggaa aaaaacaaaa
     6961 taccgtcatt ttcttcgtgg tataataaat aaaaataaat ggcggaggaa aaagaatgag
     7021 tttaattatc gatcatgtca caggggggta cggacatctc cctgtgttaa aagatatcag
     7081 tttcgatgta cagcctggag agatagtagg gttgattgga ctgaacggag cagggaaaag
     7141 tacgaccatc aagcacatta tcggtctgct ggaagcttca aaaggaacga tagaaatcaa
     7201 tcatctatcc ttaaagaaaa atacggaaga atatcgcaaa caaattgggt atattccaga
     7261 gactccttct ttatatgaag aattgacatt gagagaacat atcgaagtca ctgcgatggc
     7321 ctacgatata cctatggata tagcaatgaa aagagcagaa aagttgttgg agacttttcg
     7381 gttatctaat cgtcttgact ggttcccagc taatttttca aagggaatga aacagaaagt
     7441 gatgatctta tgcgcctttt tgatcgagcc ttctttatat atcatcgacg aaccattttt
     7501 aggcttggat ccactagcta tcaatgcatt gcttgaattg atggtagaaa tgaaaaaaga
     7561 aggagccggt attttgatgt ctacgcatat tttagctact gcagaaaaat attgtgatcg
     7621 ttttgtggtc ttacataacg gagagatacg agcagacggt tctcttgaga tgttgagaaa
     7681 agaatttcag ctgccggatt cttctttgga cgaaatctat attgctttaa caaaggagca
     7741 ggcacatgag tagttttttt caaaaacgtt tagcaagaca tcaaaagaaa atgatgaaat
     7801 atatgcgtta tgtattgaac gatcattttg ttttagtttg tcttttcttg attggcggtg
     7861 tgggattata ttattctaat tggttgaaaa ccctgcagcc accgtttgca attggagggc
     7921 tcataatctc tgtattttgg atgctctgtt tattcgtggg taagatggct acttttgcag
     7981 aggcagcaga tattgttttt ttacttccta aagaaaaaga aatgcaaacc tatttggaac
     8041 gagcttttcg ctattcttgc atttttccgt tgatcttttt gatattggca tgcggattgg
     8101 ccatgccatt agtggtggtt agtacggagc aatccttttc gatgtttttc gtctatctga
     8161 tcatattatg gagtttgaaa ttcagtcatc tgcaaatcaa gagagcagaa ttgtttcgac
     8221 tagatcaaca agttattcgt tattggaaat taggctggtt tttttcttct ctgatctttt
     8281 tgtttctatc attttattgg ctaatatggg caggaatagc aggtgcatta gtacaggcaa
     8341 gtctatatta ctggtttttg tggaagcaac aagaaaaagg attggattgg gagaaaatgg
     8401 tgcatgtaga agaacatagg ttgcatcaga tctatcaatt tatcaattta ttcacagatg
     8461 ttcctgaaat tacggcaaag atcaaaagaa gacgctattt ggatcctata ttgactcata
     8521 ttaaaagaga atcaaaaaac acttatcttt acttgtttgc aagaagattg gcaagaggtt
     8581 ctgattttag tggtctttat ttccgattag taattatagg tgggttattg attgccggag
     8641 tagtcgactt ttattttagt ctgattatcg gtttcttatt tttatatttg atcggtttcc
     8701 aactgttacc tttagcgaac cagttccgct atatggttct cgttcagctt tatccaatcc
     8761 ataagaatca gaagaagcag gcgattcagt atctactcac atgggtatta attatcgcgt
     8821 cggttatttt tggtttgatt gctgcagtcg tattagatgg gcagcagcga tgggttccgc
     8881 tagcggctta tttgattgtt actatgctct ttgttaaact atatgtacca actcgtttga
     8941 agaagatgtc agactgaaaa caaagctgtg tagggtaaaa atgagaatga aaaatagagc
     9001 gttgacagta agaatgctgt tcgttctatt ttttttgaga aaagctggtt ttttgaatgg
     9061 caattaaatg aaaaagaacg tatatgtgct tgacgtgtag tgttcaggcg actagaataa
     9121 taagtactgc aaaacaaaga gtaagaggac gggagaatgc atgatggagt ttcagttaga
     9181 ccaagagtgg cggttacgtc ccatcaaagg cgacacagga aaaacataca taggcatgcg
     9241 tgatgaggac aaagtcttta ttaaacgaaa cacgactccg atgttggctg ctctttcaag
     9301 ggaaggaatt acaccaaaac ttgtttggac aaaacgaacg gggaatggcg atactttgac
     9361 agcacaagag tggctggaag gtcgcttgct gactcctaat gagattggtc aaagaaacga
     9421 cgtgattgat gttttgtacc atttacatca ttcaaattct cttaaaacca tgctgacccg
     9481 tatcggtggt cgtgtcatga cgccggagaa gatgctgcaa aaatatgaaa aagaattacc
     9541 ggaagaactc cagaaaaatc aatttctgca aattgtccat cgttttcttc gtgagcagat
     9601 cccgtcaata gataaggaaa acttatcagt agtacacgga gatgtaaacc atcgtaattg
     9661 gttagtatgt aaaaattatt tatatctagt cgattgggat tcaatcatgt ttgcagaccc
     9721 tgcggtggat attgcaacga ttttaggaaa ttatgtgcca ctgtctagct ggaatcagtg
     9781 gctgatcagt tatggcatac gcccgactga cgaaatgctt gaaaaatttc attggtatgc
     9841 ggccatgaat ttgctccaag aaatcaccag atattgcaaa cgtgaagatc atcgacggat
     9901 gaacgaagaa attttacaat taaaacgaat tttcagtggc tgaggtttgg cccaaactct
     9961 gcggagattt ggccagacct ttttaattag aggaaaggaa tggagataaa taatgcgtgt
    10021 ccgaaatcgt ccgggagcag cggaaatgtt agcggctcac ccgaattttg taattagtga
    10081 tccaacattg tggaagggaa aatggaatga attgtttgaa aacgatcatc caatccacat
    10141 tgagattggt atggggaaag gccagttcat cactggaatg gcaaaagccc atcctgaaat
    10201 caattatatt ggagtagaaa tgcaggtcag tgttgtttcg attgcactag acaagttgat
    10261 cgaacaaccg cttcctaacc tgaaacttct acatgtggat ggatcagcgt tgacagagta
    10321 tttcgctgac agcgaagtag atcaaatcta tctgaatttt tccgatccat ggccaaaaaa
    10381 acgtcatgaa aaacggcgat tgacatataa gacttttctt gctgtagatg aacagattct
    10441 tcgtccaaat ggagaaatcc atttcaaaac agataatcaa ggattgtttg agtattctct
    10501 ggcaagtttt tctcaatatg gcatgatcct caaacaggta tggttagatt tacatcaaag
    10561 tcaatttgaa ggcaatatta tgacggagta tgaagaaaaa ttttcttcaa aaggtcaacg
    10621 tatttatcga gtagaagctc aatttcaaga taagaccaaa aagaaaagtc tgtgaaacta
    10681 actttctgct ttcataaaaa cattaatatc cataaataaa tctcagaaaa tgaacattac
    10741 agatgtaatg tattttctgg gatttatttg atttaaaatg tatttatttc atcggttttt
    10801 ccacaaggtg gaatcaattg gaagcgagaa aatgaaaata atcctttgtc ggtgatcttc
    10861 aattcgggta tgacagataa tgttaagaaa gaaagtgtca aaaaaggatc aaaggtttta
    10921 gctgctccta gttgatgtgc tttagttatt aattcattca actgttcata gacgatcgaa
    10981 aaaggttcct gtgtcatgat tccgcctata ggaagagaaa cacaagctat ttcctgctgt
    11041 ttgttgacaa cggtcatccc gccacctttt tgaatcaaaa gattagcagc atacagcata
    11101 tcctcgtcgt ttgtcccaac gacaactaga ttatgcgagt catgggcaat cgttgtcgct
    11161 atcgctcctt gtttcagctg aaatcctttc actatgccta atccaatgtt tcccgtttga
    11221 tggtgacgct cgatcaccgc aatctttaat tggtcttttt cgattgaagg aatgaatcgt
    11281 tcgccttgtc ggtccacttt ttctaccaaa tctttagtca ctagactatt cgggataatt
    11341 tcgatcacat gggcttgtga agaagaaaga agcagctcta acgaatgtgg atttaatggt
    11401 tgagcgttta gttttggcag acaacacgta tatgaatttg aatgatcagc aaataaatgg
    11461 tcttggagtt gcccagattg aacaactaat gttcccttat agaaaacttc atgaattggc
    11521 agcttggtaa gatcgtctgt taagaagaaa tcagcttgat acccaggggc aatcgcacct
    11581 agtgttttca aatcaaaaca ttctgctgca ttcaatgttg ccatctgaat ggcttgtaat
    11641 ggatcgattc caagtgcaat agcttgacgg acgatatgat cgattgatcc ttcttttact
    11701 aaatcattga tcagtttatc atcggtgaca aagaagcatc ttcttgaatt ttcaggtgtg
    11761 atcgccggta gtagcgatgc cagatctttt gcaacggtcc cttctcgaac cattaaatac
    11821 ataccaagat ccaaacgttc tttagcttct tttgcagttg tagcttcatg atccgtccga
    11881 atacctgctg caggataaac atttaactct tccatgccga taccagctgc atgcccgtca
    11941 atttttttct tatgctggtg catcaattgg atcttttcta ggatgtctgt ttcattattt
    12001 gctactgctt gatagttcat gacttcagct aagcctaaaa cttcgggttt ttgtaaaaaa
    12061 ggggctaatt tttttgcatt taaatttgca ccattggtct caaaaggggt tacaggtaca
    12121 caagaaggta gcataaaaaa gatattcatg agtgtttgtc ttgcatcttc tatcataaac
    12181 tcaagacctg tactcccagc aatgtttcct atttcatggg gatctgtcac tacagtagta
    12241 actccatgaa gcaaggatac ttttgaaaat tcgcttggtg ataaaagtga gctttcgata
    12301 tgcacatggc catcgatgaa tcctggcact agatattgcc cattagcatt atgtacgctt
    12361 tttccttcgt attcaccgat tcctacgatc attccttcgc agattgctac atctttttcc
    12421 atgatttctt tcgtaaagac gttgacgatc ttagcatgct tgatcactag gtcagcaggc
    12481 ttttttttac ttgctacttc gatatatttc cttaatgttg tttccaatgg agtgcctccc
    12541 aagataaatt tttataagta taacataaat tcggcttttg ccaatatttt tttgtaaatt
    12601 cattttattt gaaacaaaaa gatcaaaaag acgtatgttt tttgtggagg ggagaataat
    12661 ggaaataaaa cgattatcgg atcaaatgat aagtgaaagt tttgccaaac atgcccaaga
    12721 cgtatatata ttgccagctt cagaagtatt ttacatctat tttcgctatg gaaaaaaacg
    12781 aacattctgg aagaaagtct cttcggcaga tggacagcaa ctgatctcaa gatttaagta
    12841 tttgggacag atggatatcg gagaaaaaag aaaagctcag ttaggtgcat ttacttattc
    12901 tttagaaaaa caggaagttc gtctgcggtt gtcttctgta ggagattacc ttcaacgaga
    12961 gagtttagtg attcggctat tatacggtac ttttgaaaca ccccatcgtt gctttagttc
    13021 tcgagatttt gcaactattt tacagaaaac cgtcagacgt ggattatacc ttttcagcgg
    13081 gacagtcgga tcaggcaaga cttctctaat gtatcggttg gctttggaag aaaaattgca
    13141 agtgattact atcgaagatc ctgtagagat agaagaaccg ttttttctgc agctacagat
    13201 caatccgaaa atagcacaga cttatgatac acttttgaaa ctcgcattac ggcatcgtcc
    13261 agacttgcta atcattggcg agataagaga tgaatcaact gcacatgctg cggtaagagc
    13321 agcactaaca gggcaccggg tttttgcaac cgtacatgca cgcaatcttg aaggtacatt
    13381 ggtcagaatc catgaactga caaagaaaaa agaagagcta tctgaatgtt tagctggtgt
    13441 catttatcaa gaacttttgg aagatgccaa tcaccatcct tctgttttat ggagttatca
    13501 atttatggag gaagaatcag taaaaaaatc atgggaggaa agttatgaag aagctcagga
    13561 aaatatttgg cagtcagaac tattctaaag gattaacaag aagccagcaa aaccagtttg
    13621 cacaaaccat gggagaactg cttttatctg gttttagtct gcaagaatcc ctttatttat
    13681 tactcacgat ccgttcgttt ccacctgtta ttttggcaaa tatccaacat cagctaaagg
    13741 aagggcaagc gtttagtgag gtactttcgc aaataggata tggagcagag caactggtac
    13801 agatcgagtt agctgaacgt catgggaatc tgccgacaac tttgttggaa attgccaaac
    13861 agtttcggct tgttgaagga tttcgcaatg atttgaaaaa gctgcttgca tatccgatgc
    13921 tattgctcac ctttttgcta ggtatcttgt ttagtatgcg gttgttcgtt ttgcctcagc
    13981 ttattggttc aggaatggtc atgcaagagc attggggtat tcagctgata caagtctttc
    14041 attggtatgt tctactattc atcctagcgg ttactctatt ggctttacta gtaaggtatc
    14101 gtctgatgaa gttgtctcct gttgatcaga gtgaatggat aagcagacaa ttattgattg
    14161 gaccgatata ttccttgtat caatcggctt atttgtcact agagattgga aagctttttc
    14221 atgaaggttt cgaattaagg caaatcatag tctgcttaaa ggaaacgaaa caagaaagtc
    14281 tgattcagca tcttgcctct aagttgattc aaggattgga ggcaggtatg tcattggctg
    14341 agcagttcca gaattatcgt tttctatcag aagaattcag taaaattgtt ttacagggag
    14401 aggcaaaggg aagtttaggt aaagaactga ttttttacag cgaatggaca agaaaacagc
    14461 ttttcaaacg tcttcatcac tggatgcatt ttattcagcc aatcattttt ttgggcgtag
    14521 cggctttgat tttattagtt tatgccgctg ttttattacc tgtttacgga agcatagagg
    14581 aggttttgcc atgaaaaaga gaaaaatatc ttattatagc ggttttacat tgatcgaaat
    14641 gctaatcgtc ttactgatca tcagtgtatt agtgttgttg ttcgtcccta atttgtcgcg
    14701 ttataggaat catgtagacc aagaaagtcg tgaagcgatc atccagttgg tagacacaca
    14761 aaaagagctc tatgcgcttc aaaataatgg aagagttcct actgtggagg aattgttgaa
    14821 tgaaggctat atcaaacgag aacatgcaga aatctatcag cggccttgaa ggtaaggttt
    14881 tagcatcagg atatacgttg tttgaatcac tcttactgat tttattactt acaacgatcc
    14941 tttcttttcc agttctcgca ttttctgcat ggaagaatga attggcagtc tatcaatttt
    15001 tcggccaatt tgaaaaacga atctattcta cgcaaaaaat cgctattgtg aatcagatcc
    15061 aaacaggctt ttattggaat agtgaagaga atcaaatcat ttttcatgtt ccgaatccag
    15121 ataaaataaa ttgggaagta ttagatattc ctgatgagat taccttgaaa cgccatgctt
    15181 ctattacttt tgctgctaga accgggaatg aaagttcact aaaagcttat caattttatt
    15241 gggaagaaaa agagcggacg attacgtatc aattccagat agggagtggt cgctatacaa
    15301 aaagaattga ataaaacagg atttgttttg ttggagtccc ttacagcgct tatgatcgct
    15361 tctgtctgtg tctacttagt gagctttggc cagctgtttt tgcttcaggc agaaaagaaa
    15421 agtggggagg aactgctatt tattcgaatg atttatgaag atatcaagac gcagcggatg
    15481 tatcacaaaa gaccagagcg aagggaagaa atgatcccat acgatatcca aattgtcgat
    15541 aataatggag taatcgaaaa agtgtttgtc aatatggaga aagaagcgat cagtattgaa
    15601 aaaacaagta attccagcat ttacgctgat tgaatgtcta attggactga tgatattgag
    15661 cagtctattg ctcacattca atcttttgat tcagcaaacc atccaagtaa gtgaattttt
    15721 gcagcgtaaa gatcaaaaag agtggctaat atttttagca caattagaag aagaactcaa
    15781 gagcagtcac aatgtctctg tgaaaaatca gcaactgcac tacacgatcg aggataaaca
    15841 gtatatcatc gaaagatatc aggcaatgat acgtaaaaga agaacttccg gcggacatca
    15901 acctatgtta acaagtatca ctgagcttca attattggaa aaagaacaca caatttcttt
    15961 ttatgtacac tttgaaaatg gagaagatgg atatgccata tggacaaaaa acgatcagta
    16021 gaaaatgtta taagggctct attttatttt ctgtcttgct tttatttctt ttatgtacgt
    16081 ttttattgat ggcagcatta gaaagctacc gtttgtcaat ggatcttaat atacgaatca
    16141 aaaattatta tatagcaaaa ataatgaagc agatggcttt atcggaacag tctacgcttg
    16201 ccgaaagttc ggaaggagtt ttttactaca caactggatc tgtaacatat cagtgggtac
    16261 aacagtccct attcttagag gtggaagtct caccgtttat ctttcggttc acagaagaag
    16321 taaaaaatga tacagatact agtacagaat gatggaatgt tcgtgaaaac gccctctttt
    16381 ctgttataac actgttttta ataaaaaaat gtttgccgtt tcacaaataa aaaacagtga
    16441 aaacgattga aattttgtca attgttcgat agaattaaac aggtgaaaaa attgttataa
    16501 tatatctaga aatgaggtgc accatgttac cagaaaaaat ggaacaaggg tttaatcaaa
    16561 acctggaagc tattcagctt ttacagaatg cattgggaac ttccttttta gaagcctatg
    16621 tcgaaaatgc agaaaattta atagatgatt atcaagtacg tgtggtggat ggagtgccga
    16681 caaaagagac aacgcaacgg atcacagctc tttatgaaga gttgaagaaa ctttctttcg
    16741 agccagaaga atggcgcaga ctgtctcaat tacttttatt gaagggcagc cagacagaac
    16801 atttgcaggc gaaccatcaa ctcactcctg atagtatcgg ttttttattt gtttttctta
    16861 ttgaacaact atacacaaat aaaaaagcac cggttaagat tctggatatt gcggcaggaa
    16921 tgggcaactt gttattaaca gttcttttaa acctatcgaa tgcggggtac caaacagaag
    16981 gaattggtgt ggatattgat gatacattgt tggctgtcgc agcttctaca agtgatttga
    17041 cacaagcaaa tgttcaatac tttcatcaag acgggttgca ggaattatta atagatcctg
    17101 tcgattttgc aatcagcgat ttgccaatcg gttattatcc gaatgacgaa aaagcaaaag
    17161 agtttttaac aagtaccgaa gaaggccata gttatgcaca tcatttattg ctagagcaat
    17221 caatgaaata tgtgaagccg gatggttttg gtttgttttt gatgccgagt ggatttttag
    17281 aaacggatca aagtgaagaa atcaaaaaat ggtttaaaga agaaggttat cttcaaggaa
    17341 tgattcaatt gccagatgaa ttgttccgta ataaacaatc gcaaaaaagt attttgatct
    17401 tacagaaaaa aggaccacaa gcacaacaag taaaagaagt cttgcttgtg aaacttgctt
    17461 cattaaaaga accagagaag gtgacggaat ttttcaacga gtttaaaaac tggaaatcat
    17521 ctgctctgta atcattcaac gagaaggaga gtcatatgtc taaaacaatt gcaatcaatg
    17581 ctgggagttc aagtttgaaa tggcaattat atcaaatgcc agaagaaacc gtagttgcga
    17641 aagggatcgt cgaacgtatc ggtttacaag attctatttt taccatcaag tacggcgata
    17701 atcaaaaatt cgaacaaatc atggatatcg aaaaccacga agtagcagta aaaatgctgt
    17761 tagatcagtt gattgaactg caaattttaa gctcttatga tgaaatcacc ggagtagggc
    17821 accgtgttgt tcacggagga gaaacttatg gggattcagt agtgatcgat gagaaagtaa
    17881 tggaccgcat tgctgaatta gcagaatttg ctcctttgca taatcctgca aacttaatgg
    17941 ggatcaaagc gtttaaaaag attttacctg acattttgag tgtggctgta ttcgatacat
    18001 ctttccactc aacaatgcca aagcaaaatt atttatatag cttaccggtg tcttattatg
    18061 aagatttcaa agtaagaaaa tatggcttcc atggcacaag tcatcgttat gtgtcggaac
    18121 gtgcggcaaa attattgaac aaaccgatcg aagaactaaa aatcatcact tgccacttag
    18181 gaaatggtgt atcgatcaca gcagtcgatg gaggtaaatc catggatact tctatgggat
    18241 tcacaccatt agctggggtg acaatgggta cacgttcagg tgatatcgat ccagctgttc
    18301 tcccatattt aatggaaaaa ttagagattg atattgatga aatgatcaat gttttgaaca
    18361 agaaatctgg tctgctaggc ctaacaggat tgtctagtga tatgcgtgat cttgaaaaga
    18421 actatgaaaa agaacatatt cgtttagctt atgacatttt cgtggaccgc atccgaaaat
    18481 acatcggcgg ttatgtgaca acgatgaatg gtgttgatgc aattgtcttt actgctggta
    18541 tcggcgagaa tgacggacat gtacgtagtg aaatcatcaa aggtatgtct tggtttggtt
    18601 gtgaaatcga tccagcgttg aatgaattgc gtggtgaaga agtagatatc tctacaaaag
    18661 aatcaaaagt aagagtatta gtgatcccaa cagatgagga actaatgatt gcccgtgatg
    18721 tggagcgtct gcgcggctaa gccaaagcat tcgttaggat aaaagatgaa acgcatgcaa
    18781 gagggaattc ttgcatgtgt tttttttgcg gagaagaccg caaaatggta atttaacttc
    18841 aatttccctt cttttttaca ggatcttctt agggaatttt gcttggaaaa gtggtcaaaa
    18901 gggaaaagac tggcatttta tttattgatt ttcaggaaaa tcccaatatt ttattttcat
    18961 tcatctttat aagggacatt acatatgtaa aaagaaaaaa catttttaga tcagcctcgt
    19021 gggctgtcga ctttattctt cgctttatgt ttagctagaa aaccgatccg aaacattcat
    19081 tgatcgaata ataaaaactt gattttccac ctgtactttg cctgaatcgt gaaagcacag
    19141 gtatgaaaat caagtttttt gttgcgaaaa aacgtcctag tcaatcagtt ggccgagcgg
    19201 ctggcggctg atctgctcct gatcaataac gagtgcttct tggaagtgtt gctgcaaaaa
    19261 gtagcggtca tggctgactg ctaggagcgt tccttgatag tcttgcagca tttcttcaat
    19321 ttcttctcga gcatagctgt cgaggtgatt ggttggttca tccaaaatca ggacgttgat
    19381 tttttctaaa aagagtttca gcaacgccaa acggattttt tcaccaccgg ataaatcttt
    19441 gatccgttta cccacatcgc tttgataaaa gccacttttg gctaattgcc gtcgagcgac
    19501 ttcttcgttt ccagtcaatg tgatggcgta agccagcaaa cggctatcgg gatcggcaaa
    19561 ctgcagctgc tgcggcaaat aaccgatttg caagctacta cctaatttga tggtgccacc
    19621 gtaaggcttt tcctcaccga gtaataattt gagtaaggtt gattttcctg caccattgtc
    19681 gccgatgatg gctaaatgat ctccacgata gactgcaaaa ctgctgtctt tgaatagtac
    19741 ttgatcagcg tactgctggt gcaagttttg aatgatgaaa acttctttgc cagattttcc
    19801 accattagac aagctttgca gccgattttt cgggggaggt ggcggtttga cgagtgtcag
    19861 tttcgccagg cggcgttcca gttctttggc ttttttgaag aaggactcat tgtctccttc
    19921 atgtgcccac tgacgaaaac gacgaatcat gactttcaac cgctggactt ctttttgctg
    19981 cagttcgtaa ttttttgtca gctcagccaa gcgagcttct ttcaagactc gaaaacggct
    20041 gtagtttccg ggatactcaa tcaatgcacc ttcttccagt tcccatgttt ttgtggtgac
    20101 ttgatcgaga aattgccgat catgagaaac gaccagaacc gatttttttg tattctgtaa
    20161 atagttttcc aaccagcgaa tactgttaag atctaaatga ttggtcggtt catcgagtaa
    20221 aagtaaatcg gcatcggctg ctaaaatttt ggctaaagcg actttaactc gttcgcctcc
    20281 gctaagttct gttaaccgtg cttcctgaca gtgtggaatc cctaagcctt gcaagatcgc
    20341 tgcaatccgg tcttctaata cgtaaccgcc tagttcttca aagcgatgct gcagctcacc
    20401 gtaacgggcc agacacttct ctaagtttgt atctggttct gtcatcaatg cttcgtagcg
    20461 actcatttgc tgtttgaggt cctgtatctc agcggcagaa tggagcaagt agtggttgac
    20521 ggtcgcttcg gaaactgtaa gttcttgttc gaccgtcccg atctttaagc cttttttgcg
    20581 actgataaca ccgctgtcga caccttctct gccggtcaag atttgtaaaa aggttgattt
    20641 gccagttccg ttgatatcga ctaaggcaat tttttcacca gcttcaattt gacaggataa
    20701 atcctcaaat aatggattcc cgccgattgt ttttttaata gaaattgctt gtatgatcat
    20761 gatctctctc cttgagacca ctcaaaaagg ccatggttat ccctggcctg tgttatttaa
    20821 actcagatta tcatgagaaa aataccttga gatggccatt gattttacgt gaattcaact
    20881 gttttggaca aacttgtccc gtcaacagca actgtaaaac caattgcatg gctaaatgcg
    20941 atatggtatc gttggtaatt ttcattactt gttattttag tcatgcgcat catagtattt
    21001 tccctccttc gtgtagttgt cttttccagt gtacctgaaa gtgtcagaaa gttcaactag
    21061 cgctgacatt tacttaatat ttatcgatgt gtaattctgt cagagagctt tttttatctg
    21121 actgagggta atgtctcgtg gaccacatca ttgtattctg agctaaacca gcgttcaggc
    21181 gaaatcatat ggtgtttgat aaaaaacttt gtctacatga cttccttttg ccatttgctt
    21241 aataccgttg cattaaacag ctcttctctt tggaaatctc accaacatcg cgttcaggag
    21301 tatcgttgtg gttgcaatgg caagcggtag cctttcatca attcttgaat gatttaccga
    21361 atgtagcact tgccttctcg cggtacccga acccgaaata aaattttagt ttggcgataa
    21421 aatcggtatc cgccatcgat acttcatatt ttttcgtatc agattcatcg gaaacttcca
    21481 cattgacgaa ccaataagct ttggcatgct taggtcgttt taaaccacac cagggcgact
    21541 aatcgccctg gtgttcactt tttttatttt acttgaactc attgactgtt ttccctttat
    21601 tttaaacatt ataaagaccg atgagtgctt gccgcgtctt ttgtttttat cacggttttt
    21661 tgtgcaaaaa aacctgccga tagttggcag gtcgtctacg tgttaagctt tggtgtggtc
    21721 tttagctggc aattggtttt ctaaatcagt tcggacaaca agcacatcgc aagctgcgtt
    21781 tctaataaca tattcagaaa cagaaccaat aaataaacgt tctacagcat tcaatcctgt
    21841 ggctcctaac ataatcaaat ctacttgatt atcttctggg atttctctgg caataatttg
    21901 ttttggtgag ccatattcta cgacagaggt tacattattc aagccagctt tcttcgcatt
    21961 gctttcataa tctgccaatg tttgctttgc catttcagta gcttgttctg caagcattcc
    22021 atcaaaggat gaaacggttt ggaaagaacg tgtgtcaatg acatgtgcta atagaagttc
    22081 tccgttattt cggatagcta cattgaccgc ctttttaaaa gctaattcag cttcatcaga
    22141 accatctacg gcaaccatga tttttttgta ttgttgtaac ataagcattc actcctcaag
    22201 tcaatcgtat tgtactcttt tattgtagaa cactttaggg aaaaagagaa ttatttactg
    22261 ctttggttca gcaatttttt ttgcaaatcg gatactgaag gtggcagaaa caataattac
    22321 gatcagtaaa aagaccaagg caataaagcg ttgattgaaa aaagctgtta aaatacccat
    22381 tacgcctaaa acagcatatg cagcaccgta aaattgtagg aatccttgat ttttatcatt
    22441 tttgttgatc aatacaaaaa agatatcctg tttattaaat aaataatagc taacgaataa
    22501 taatactaaa gtggcgataa tcattaaaat cctaatcatg aacaaatcct ccaaaataag
    22561 caacaaatag aaaaagcaat ttgccaagag caaattgctt tattctttaa aagatggtaa
    22621 tctccgttaa tgccgttgtt tgtcctgtag tattgatccc gcgaattcaa gcaggtgggt
    22681 cccatggtcg tagcttcgaa ctaccaaatg ataaggcatg ttaccagcag tgacacaaga
    22741 cgggtcccaa gatatcaata aaaatgttcg gtcaacacac aaagaggaca attcgaacat
    22801 cacagatttc ccacctctat agtagcatat gaatttaaaa agagcaatga gagagctcgc
    22861 agaaacagaa ccgttttctt gtctagtttt ttggaggatg ctttttccac tcttttatac
    22921 ggtaatactg ttgacccaga gcctgttcat atttccctgt atcttttggc tgataatact
    22981 gagcattctt cagtttatct ggtaaatatt gctgattgac ccaagcctga tcaaaatgat
    23041 gaggatactg ataacccacg ccacggttga gtgatttcgc acctttgtaa tggctatctc
    23101 tcaaatgatc aggaacatct ccagctttgc cctctcgaat atcagcaagt gctgcatcta
    23161 atgccatgta ggcagaattg gattttggcg acaagcaaag atcgacgacg acgtctgcta
    23221 gtgggatacg tgcctcaggc aatcccaatt tttcagcagc taatacagcg ttcactgtac
    23281 gagccgcagc agcaggatta cctaaaccta tatcttcata accgataacc atcagacgcc
    23341 ggcaaatact tgccagatca cctgcctcaa ctaaacgggc gagataatga agtgcagcat
    23401 ccacatcact tccacgaatc gatttttgaa aagccgaaat aacgtcataa tgagcatctc
    23461 catttttgtc atgggtaaga gcttttcttt ggacacattc ttcaatgatc gacagtgtaa
    23521 gatgaatcct tccttcttta tctgaaggag tcgatagtgt agccagttcc agcccattca
    23581 atgcactccg caaatcgccg ttcgttgcac gcgaaaggtg aagaagagca tcttcgtcta
    23641 actggattgc ttgttgtcca aggccgcgtt ctttatccct taaagcatgt tcgactgcca
    23701 actggatgtc ttgttctgtt aatggtttga cttcaaagat ctgtgtacgg ctgcggatcg
    23761 ctggattgat ggtaatataa ggattctcag tagtcgctcc gatcaagatg attcggccac
    23821 tttctaaatg aggaagcaga aaatcctgtt ttgttttatc taatcggtgt acttcatcca
    23881 ataagaggat cactgtacca ctcatttttg cttcttccgc tacaacttgt agatcctttt
    23941 ttgaatctgt agcggcgttt agcatgcgaa atgcataatt agtagagccg gcgatcgcac
    24001 ttgcgatact cgtttttcca gtaccaggag gtccatatag aatcatggaa gagagcatgc
    24061 gagcatcgac catgcggcgt atgatttttc cttccccgac tagatgctgc tgaccaacga
    24121 cttcatcgat cgttcgaggt ctcatgcgat aagccaatgg ctgttgcata ggattttcct
    24181 ccttcttgtt tttacgtccc ttaattatat catatttagc aagatgccta gaaagaacag
    24241 ccttctgttg ttttgttttc atagcttatc caactaatat tgtgtatgat aaagtaggtg
    24301 agaaaaatga cggataaaca aaagatagat gaattttttt ggaataaaac gacgccagaa
    24361 gtggcacggg atttattggg gatgtatttg gagcatgaca caccagaagg aagattagct
    24421 ggatatattg tagacgcgga agcataccta ggtccagaag acgaggccgc ccatagctat
    24481 ggtttaagaa gaacaccgcg tgtgcgggca atgtatgaaa aaccaggtac gatctacctt
    24541 tacacgatgc atactcatcg gattttgaac atcattactc agccagaagg gattcctcaa
    24601 ggagtaatga tacgtgcaat cgaacccgcc gcaatgattg atcaaatgag taaaaatcgt
    24661 ggcggaaaaa ctggtccaga tatcagcaac ggtccgggaa aattagtgga agcattagca
    24721 attcctcaag aactttatgg tcagtctata gcagatagtt cgttacgctt agtttttgag
    24781 aaaaaaaaga caccaaaaaa gatcaatgct ttgcctagaa tcggtatccc aaacaaaggt
    24841 gtatggacag aaaagccgtt gcgttttgtt gtgtcgggaa atccttatat ctctttacaa
    24901 agaaaaaacc aaatcgagaa agattggggt tggaggaaag aaaatgaaaa agaaggatct
    24961 attaacatat ttagatgaaa taattgaaaa acaagtgaca gactatgatg tagctttaga
    25021 ttgggataca aaaaatcata cgatcgagat cgttgttcgc ttgtttgcag aaaataaagc
    25081 acaatttgag attgatgatg cagaagggat cgtttcgcaa gaaccaatta tcgaatttga
    25141 agacggcgta cttcttttta atcctcagaa atctgttttt gatgaacgca attatctagc
    25201 agtaatccct tatgaaggaa aaaaagggtt ggcaaaatca gtagctgatt ctttagtgga
    25261 atatctaaat gaagtattag ctcaagggca aagcgatttg cttgattttt tggatgaaga
    25321 cgatgacgaa gctgtattcg agcttcactg ggataatcaa caactagcag aattagtaga
    25381 agtaaaacag caaaatgaag cagatactta tttaccgtac ccaagttatt aaggagtgac
    25441 agatatgaaa tggaatgaag taaaagtaga aactgaaagt gaagcagtcg aagcagttgc
    25501 taatattttg atggaggcag gggcgagtgg tgtcgcgatt gaagattctt tagatgttga
    25561 acattttcag tcggatcaat atggtgagat tttagataaa aatgatttta catcgctgaa
    25621 agaaggcgca ctagtcatgg cttattttcc ggaaacgact ttcttaccgg aaatcttgcc
    25681 ttttatcaaa gatcgtatca cacaattacc tgagtttggt ctagctattg gaaaaaataa
    25741 agtaacagtt agtgaagtag aggaaagcaa ctgggcaaca gcctggaaga aatactatca
    25801 tccggtgcgt attacgcgaa tgctgacggt tgcgccaagc tgggaaaact accagacaga
    25861 agatcctcta gaaaaaatta tcacactaga tcctggtatg gcatttggca caggcactca
    25921 tcccacgact tccttgacat tgacagcact agaaacagtc ttgcgtggtg gtgaaacatt
    25981 gctagatgtg ggaactgggt cgggcgtgct aagtattgca gcaaaatatc ttggtgcaaa
    26041 agatgtctat gcctttgatt tagacgaagt agccgttcgt tcggcgaaag aaaatatgga
    26101 tatgaatgaa atagccaaag atgtccatgt atcagctaac gatcttttga caggaatcga
    26161 gattgaaagt gatgtaatcg ttgcgaacat tttagcagat atcatcttat tgatgatccc
    26221 agatgcttgg cgtttactga agcaaacagg cacgctgatc gtttcaggaa tcattgaaga
    26281 gaagaagcaa atggttctgg atgccatgtt agaacaaggg tttatcgtgg atcaaatttt
    26341 ccagcaaaaa gactggtatg caattatttt gaaaaaacca gaggtggaat agatgcagcg
    26401 gtatttttta aaagaatcat atgaatcaaa agactatttt gttttagaag gggaaaacta
    26461 tcaccacatt gtacgggtta tgaggatgga acccgaggat gaagtttttt tggcattcaa
    26521 tgatcaaata gccattgttg cagaaatcac ggagattact gaagaggctg tttatctgaa
    26581 agaaattaaa aaagaagaaa atcaaaagga actgccaagt tcgattacca tagcgagtgg
    26641 ctatcctaaa ggggaaaagc tggattggat cgttcagaaa tccactgaat taggagcgca
    26701 agcttttctt ggtttcccgg catcagcctc tgtcgttaaa tgggatgcaa aaaaacgtat
    26761 gaaacgacaa caacggcttg aaaagattgc tcaagaggca gcggaacaat cccatcggca
    26821 gatcgtgcca tcagtagagt tattggaaac gaaacaagat ttatttgagc ggctagatga
    26881 ttatgacgtg gtcttggttg cttatgagga atcagcaaag caaggcgaaa aatcaaatgc
    26941 agccaaaatt cttagtgaaa caaaaccaga tagtcgaatc ttagctgtct ttggaccaga
    27001 aggtggatta tcccgcgatg aagtcgatta ttttgtagaa cacaaaggga tactttgtgg
    27061 attaggacca agaatccttc gtacagaaac agcacctctt tatttattaa gtgctgctag
    27121 ctatcaatgg gaattaatga atccttcttt cagctaacgt tcttcattaa taaaagagta
    27181 tcgacagaaa gaattgaaaa aatagtcaac aatttgtata atagaaacaa ttatttatac
    27241 aatggatacg aaaaatcgtt tgaaaaagct tttaaaaagc gaacatttcc gtaaaaaaac
    27301 gtataactgc acggattttg ggacaattat caaacagata tttgtcccga ttcccgtttt
    27361 accgaaaaaa gaaaggggga tcgattcctg tgacaaaaga agaaatttta acgggacccg
    27421 gtgtcataaa gctcgttagt caatacatgg gtcccgaaca tgtagcattt gttgagaaag
    27481 catgcgaata cgcgactgct gcacatgacg gacagtttag aaaatcaggc gaaccctaca
    27541 ttattcatcc tatccaagtc gcaggtatat tagcagattt aaaaatggat ccccatacag
    27601 tggctacagg cttcttacat gatgttgttg aagatacaga aatcacttta gaagatctga
    27661 gagaagaatt tggcgatgac gttgctatgt tagtggacgg cgtaaccaaa ttagggaaaa
    27721 taaaatataa atcccacgaa gagcagctag cagaaaacca ccgaaagatg ctgctagcaa
    27781 tggctcaaga tttacgagtt atcatggtca aattagctga tagactacat aacatgcgta
    27841 cgttgaagca cctgcgagaa gataagcaaa gaaggatcgc tcaggaaact ttagaaatct
    27901 atgcgcctct tgcacatcgt ctagggatca gccggatcaa atgggaatta gaagatacag
    27961 cacttcgtta tctaaatcca aaacagtatt accgtatcgt ccacttgatg cagacgaaga
    28021 gagaagaacg tgaaaaatac gtaagtggta ctgttgaaga tattcgaata gcgacagaag
    28081 agctggggat ttttgcagaa atctatggac ggccaaaaca catttattca atctatcgta
    28141 aaatgaaaga tcagaaaaag cagttcaacg aaatttatga cttgctagct attcgagtga
    28201 tcgtagattc aatcaaggat tgttatgctg tattaggagc aatccacaca aaatggaaac
    28261 caatgcccgg cagatttaag gattatattg ccatgccaaa agctaatatg taccaatctt
    28321 tgcatactac cgtgatcggt ccggcaggaa atccggtaga aattcaaatc cgaacacagg
    28381 aaatgcatga aatcgctgaa ttcggggttg ctgcacactg ggcctataaa gaaggaaaaa
    28441 atgaaaaagt agaaccagat ggtatgacga aacaattaag ctggttccat gagatactcg
    28501 aacttcaaga cgaaagctat gatgcttctg aatttatgga aggcgtaaaa ggagatatct
    28561 ttagtgataa agtctacgtc ttcacaccaa aaggagacgt tactgagtta ccaaaaggat
    28621 ccggaccatt ggactttgca tacagtatcc atacagatat cggtaacaaa accactggtg
    28681 caaaagtaaa tggcaaaatg gtgcagcttg attacaaatt gaaaaacgga gatatcattg
    28741 agatcatgac ttctccaaat tcatttggcc caagtcgcga ctggttgaaa ttagttgcta
    28801 ctagcaaagc aagaaataag atcaaacgtt tcttcaaagc ccaagatcga gaagaaaatg
    28861 tgatcaaagg ccacgaatcc gtggtcaaat gtattacaga tctaggattt acgcctaaag
    28921 atattttgac gaagaacaaa ctgcaagaag cactcgatcg ttttaattat caaacagaag
    28981 atgatctcta tgcagctgta gggtatggag aagttagccc cttgacgatg gccaatcgtc
    29041 tgactgaaaa agaacgtaaa gaacaaaaaa tcgagcagca aaagcaagaa gcagaagaaa
    29101 tcatgaatca gccgaaaaaa gaacctgaca aaatgaaagt acgtcatgaa ggtggcgttg
    29161 tcattcaagg ggtagaaaac ttactaattc gtatcagtcg ctgctgtaat ccgattcctg
    29221 gtgatgatat cgttggttat atcactaaag gcagagggat atccattcat cgtcgagatt
    29281 gtccgaatgt tcagcctgac aaaccaaatg tagcagaacg tttgattgaa gtcgaatggg
    29341 aagatacatc gaatacacga aaagagtatg atgcagattt ggaaatttac ggctataatc
    29401 gttcaggctt attgaatgat gtacttcaaa cagtcaatgc gctaacgaaa aatctcaaca
    29461 gcgttgaagc acggacgaat aaagataaaa tggcgacgat ccatttgacg gttggtatcc
    29521 agaatttatc ccacctaaag agtatcgtgg ataaaatcaa agcagtacct gatgtctaca
    29581 gtgtacgccg gacgaatgga taggaggaca gatatgagag cagttgttca acgggtaagt
    29641 aaagctgctg tgtcagtcga tcagcaagaa attggaaaaa ttggctacgg cctacttgtt
    29701 ttattaggca ttcatgaaac cgatacgcaa aaagatgtgg actatctggt aaaaaaaatt
    29761 gcacaaatgc gtatttttga agatgaacaa ggaaaaatga atattagtgt agaagatgtg
    29821 ggaggacaaa ttttaagtat ctctcaattc acactgtttg cggatacgaa aaaaggaaat
    29881 cgaccaagct ttgtggaagc cgcacgacca gaaacagcta ttcctttata cgaatcattc
    29941 aatgaaggaa tcagaagtcg cggcatcaca gtcgctaccg gacaattcgg agcagatatg
    30001 tcggtttctc tcgtcaatga tggacctgta acgatcatta ttgattctaa aaacaaataa
    30061 agtaatctgg ttttcactag taaaaactat gaaacacgtc gcttaagtta gactgataaa
    30121 ctctaactta agtggctttt ttgtctgcaa aaagcgcttc ttttgtcagc agtcggaaaa
    30181 aaagttaagc ttaaattata caagttattt tttataggag gttgctgaag atgaaagaag
    30241 taccagtagt agatatcaaa atcacagatt taaaaaaatt gtatcaaaaa ggatacaaca
    30301 tgttggagga gttacgccat gaagcggacg ctcccgtcgt caaagcaaag attttcaata
    30361 aagaagctat cacgatctac ggatcatcag cagcaaaagt attttatgat ccaaggaatt
    30421 tcaaacgaaa aggtgctatg ccaaaattag tattgaagac actttttggt caaggtggag
    30481 tacagacttt ggatggagct gctcatcacc atcggaaaaa tatttttatg gatctaatga
    30541 caccggaaag aatggaagat tatcatcgta tcttagataa gaatctgaca caagcacttg
    30601 aagcacagca tggtcaattt gaactatttg atttaagtaa gatggttttc tttacatcta
    30661 tttgcgagtg ggcaggtatc aatttatcag cgatttctaa agatgaggtc gaaaaattag
    30721 cagaatatca aatctctatg atcagcggaa cgtttacttc tccgattgat cacataaaag
    30781 gtgtcgagaa tcgcaaaaaa tcagaaaaat gggcgcaagg cttgattgaa gaagctcgac
    30841 aaaatcctgt ggctggaaaa gaaaatgtgg cattatatgc ttttgccaat gccacagatt
    30901 tagatggtca gcttttacct ctggaagtgg cagctgttga attattgaac atcatccgtc
    30961 caacggttgc tctaactgtg tgggcggcat tgatgggaca tgcacttttc agtagaccag
    31021 atttgtatca gcaattaaaa aatgactttt cgacgttaca agatccgttt attcaagaaa
    31081 tgagacgcta ttatcctttc ttcccaatgc tacctgcgat ttcgctgaaa gaagtagaag
    31141 tagatggtta tcgtatacca gaaggcagtt gggttatttt agatttatat ggaacagacc
    31201 atgatgaacg aacagttgaa gcgccagatt cctttatgat caaacggtat gtagggaaag
    31261 caaaagatat ttcctataaa gaagaatatg agatgattgc acaaggcggg ggaaattttc
    31321 gacaaatgca tcgctgtgca ggagaatgga tcactcttca tagtctacgt gtgttttccg
    31381 atcagctagt aaataagttt gagttctctg tacctgaaca agattggacg attccattca
    31441 atcagtttcc gacttatccg aatagccgag cgctacttta caagaattag attagcataa
    31501 acttatcgaa aaaaaggaag atcaaacagt gctgatcttc cttttttcat attactataa
    31561 attgataagg tcaattgatc aaaatccacg aaatgcttca tcaatggttt gataatcttc
    31621 attggataaa tgcaaatcta aagcttggac attgtcagat acttgctctg gacgcttagc
    31681 tccaggtata actacagaca ctcgtggatt cttcatgtac caagctaaaa caagttgcgc
    31741 aggagtggct tggtatcttt tagcaatggg gcgaagtaca tcgacagcgg ttaaaatttc
    31801 tctgaaacgt tctccttgga aatctggatt tctttttcga ggatctcctt caccaaattg
    31861 tttttcctca ccaagttcat attttcctgt cagcaaaccg gaagcaagag gaaaataggg
    31921 aacaaaactg attttgtttt tttcaagata agggaataat tctttttcag cctgccgatg
    31981 gatcaaactg tatttatctt ctactacatc gacatatcca tctgcattcg cttctttcaa
    32041 ttgttctaac gtgaagtttg acacaccgac tgctcggatt tttcctgctt cttttaattc
    32101 gtgcaatgta gcaactgatt cattttttgg tgtgctttca tccggaaagt ggatataaaa
    32161 aatgtcgatg taatcagttt gcagacgttt tagtgcatct tccaccgatt gtttgagaaa
    32221 ctcaggagaa ttatcaaatg ttcgcccttt atttggtaca tgagcagctt ttgtagcgat
    32281 gacgacacgt gagcgatcat attctttttc cttcagtacc tcgccaatca gttcttcgga
    32341 acggccgttt ccgtaagcat aagcggtatc gatcaaattg atcccgctat tcaaagctgt
    32401 acgaactact tgttttcctg tctcatcttc caaaccgtca aaaagattat gtcctccaac
    32461 ggcatttgct cctaatccaa gctgttctgc gtagacttgt gaatgtccga ttcttatttc
    32521 tgccatagct gattcctcct ttttgtctag tctaaaacaa aataccaaaa agctgaacta
    32581 aaacgttttt gtgaggggaa atagtcagat atctcttgac tttgagactg gtttttagta
    32641 ggttataagt aatatcgaat gtcaatgaaa gagcgagtaa gtttttgaaa ctttacaaga
    32701 gaggatcatc gaggctgaaa atgatctaaa gtttcattag cctaaagaca ctctggagat
    32761 ggacgagtga aaagccgtcc cgcgtacgag cgttattcgt aagaggaaag agaaatcttt
    32821 caaaattagg tggtaccgtg tatgaatttc attcaccctt gttcatgatg aacaagggtg
    32881 atttttttat ttacatagct aagaaaggga gagaaaacta tgagtttcca aagaccaaaa
    32941 ggaacaaatg atattttgcc aggcgaatca gaaaaatggc agtttgtcga agaaacagca
    33001 cgtttattat tcagagatta tcagtataat gaaatgcgta caccgatttt tgaacactat
    33061 gaagtgatat cccgaagtgt aggggataca acggatatcg tttcaaaaga gatgtatgat
    33121 ttttatgaca aaggggaacg ccatgtgacg cttcgaccag aaggtacagc accaatcgtt
    33181 cgttcttttg ttgagcataa attgttcggc ccagagtatg cgaaaccgtt caaaactttt
    33241 tatatgggac caatgtttcg ctacgaacgc cctcaaaaag gacggttacg tcaattccat
    33301 caaatcggag tagaagcatt tggatcagaa aaccctgcaa ctgatgtgga gagcatggtg
    33361 atggcacttg atttcttcaa acagttgggg atcacgcaaa tccggctagt catcaattca
    33421 ttaggtgata aagaaacgcg tcaagcctat cgtcaagcat tgatcgacta tcttttacct
    33481 tttgaatcag agctgagtga agactctaaa cgccgccttc atgaaaatcc attacgcgta
    33541 ttggacagta aagacaaacg agatcaaaaa tttgtggcag aagctccatc tatcttagat
    33601 tatctaagcg agcctgctca aaaacatttt gataccgttg tttccatgct ggatgcattg
    33661 gctgttcctt atgaaatcga cagtaatatg gttcgcggac tagattatta cacacatacg
    33721 atttttgaaa tcatgagtga cgcaccaaaa atgggggcac aagcaacgat ttgcgcaggc
    33781 ggacgttatg acaatctagt ggaagaacta ggtggtcctt caacacctgg cttcggattt
    33841 gcaatgggta tcgagcgatt actcttgact atggaggcag aggaagtagt tatccctgca
    33901 tttaatgagt tggatgctta cgtggtcgca ctcggagacg aaacaaatat cgaagcattg
    33961 aaagtggtcc aagcgattcg aaactttggt ttttcagctg accgcgactt catgaaccgt
    34021 aaagcaaaag cgcaatttaa gacagcagat aaagcgaatg ccaaattagt attggtgatc
    34081 ggtgaagatg agcttgctag tcaaacgatt aatgtcaaag caatggcaag ccgaaaagaa
    34141 aaaagttttc cattagaaga gatatatgaa cattttgatg aagtctatga tgaaatgact
    34201 ttattcgagt aggaggaaaa aaataaatgg cacaaagaac gatctattgc ggattagttt
    34261 ccgaacaaca attagaacaa acagttacat taaaaggatg ggtacaaaaa agacgagacc
    34321 ttgggggagt aatctttatc gacctacgtg atcgtgaagg aatcgttcaa atcgtcttta
    34381 atcctgaaat caacaaagaa gcatgggaaa tcgcggataa atgtcgcagt gaatacgtga
    34441 tcgaagtgac tggggaagtt agaagacgcg gagaaaatgc agtaaatcct aaaatggcca
    34501 ctggtgaatt cgaagtgatt gcaagtgaaa tcgtgatttt gaatcaagca aaaaccccgc
    34561 catttcaaat tgaagatgat caagcaatca gtgacgaaat acgtttgaaa tatcgttatt
    34621 tggatctgcg tcgtccaaaa atgactaaaa acatcaaatt aagaaatgcg acaacaaaag
    34681 cgattcgtca ttatttagat gataatgact ttctcgatat cgaaacacct tacctaggga
    34741 aatctacacc agaaggcgca agagactacc tagttccttc acgtgtccat gctggacatt
    34801 tctatgcgtt gccgcaatct cctcaattgt ttaaacaatt gctgatgaat gctggattcg
    34861 accgttatta tcaaatcgtt cgttgtttcc gcgatgaaga tttacgtgga gaccgtcagc
    34921 ctgaatttac gcaagtagat attgaaacaa cgttcttaac agcagaagaa atccaaactt
    34981 acacagaggg attgattgcg aaggtaatga aagaagttcg cggtatcgaa gtcacattgc
    35041 catttcctcg tatgacctat gatgaagcaa tggctcgcta cggcagcgac aaaccggata
    35101 cacgttttga tatggaattg atcgatctaa gcgatacagt caaagaagta gaattcaaag
    35161 tcttccaaat ggctcttgaa aacggcggtg tcgtgaaagc attgaatgct aaaggcgctg
    35221 cagatcgcta ctcacgaaaa gacatggatc agttaggtca atatgtagga caattcggag
    35281 ctaaggggct tgcatggctg aaagtagaag aagacggatt gaaaggtcca attgctaaat
    35341 tcatgggaga agcaacagaa gcaatcatta aagcaacaga tgcaaaacca ggtgacttgc
    35401 tgatgtttgg cgcagataaa agtgaaatcg tcgctgctgc attaggtgct atccggacac
    35461 gtttaggaaa agaattagga ctaattgatg aatcgaaatt caatttctta tgggtaactg
    35521 attggccaca atttgaattt tcagaagaag aaggtcgata tgtctctgct caccatccat
    35581 tcacgatgcc aaaagaagaa gatatcccgt tattggctga agaccctgca aaagtctatg
    35641 cagaagctta tgatatcgtc ttgaacggtt atgaattagg cggaggttct atccgtattc
    35701 atactcgtga gcttcaagaa aaagtactag aaactctagg ctttacaaaa gaacaaatgg
    35761 aagaacaatt tggtttcttg ttaacagctt tagattacgg attccctcca catggcggta
    35821 ttgcattagg attggaccgt tttgcaatgt tattagccgg agaagataat attcgtgaag
    35881 tcattgcctt tcctaaaaac ggaaaagcag cagatgtgat gagtgatgca ccaagtatgg
    35941 tttcgccttt acaattgttc gaattgaata ttgatgtaac gaatatagaa gaatagccga
    36001 taaaaaggta aaaaggattt gtcgaatacg cttcttttga caaccttgat ttaattgaaa
    36061 aacactttat aatgaagcca taacatataa agcggggctt ttccatgaaa aaaacattcg
    36121 aaaattgggc ttatcatgat tacacgacca agctttcggt atctatcgtt tatgcgttga
    36181 tcgcatcagt cgcattgaat ttcttttatc agccaggaca tatttattca agtggtatta
    36241 ctggtctagc tcagattttg acgactttgt caaaatcact gataggattc gaagtaccgg
    36301 tatcaacaac tttgtattta ctgaatatac ctttgtttgt attggcgtgg ttaaaaatcg
    36361 ggaagaagtt tacaattttc acttttatca cagtcacttt gacctccctt tgtatccata
    36421 ttttaccgga aacagtccta tctaccgatc caatcatctg tgcgttgttt gggggggctg
    36481 tgatgggact aggtattggg ctagcgttga gaaacggcct atcatcgggc ggtctagata
    36541 tcgtgagtat cacgattcgt aaaaaaacag gaaagtcgat tggttctatt tcgatctact
    36601 tcaatgcatt gatcgttttt gttgccggaa ttctatttgg ctggcaatat atgttttaca
    36661 gtacgttgtc tattttcgtc agtggaaaag taactgatgt cgtttatact aaacaaaaga
    36721 agatgcaggt aatgatcgta acgaaacatc cagaaaaagt agtaaatgaa atccaaaact
    36781 gtatgcgtag agggatcacg atcatcaatg aagcagaagg tgcttataag catgataagc
    36841 agacagtatt gatgacgatc gttacacgat acgaactgcc tctacttaga gaagcaatga
    36901 aagcttctga tccacaagct tttgtaagta ttgcagaaaa cgtccagata cttggtcgtt
    36961 tctatgagga tggcatttag atcgagtttg acaaaaattc atgaaatact gctgtagaca
    37021 gacgttcgtt tgtctgcagt ttttttctac catagtagaa tcagcaaaga aaaactgctt
    37081 ttttatttga aattttctta aagttgaaaa aaagcatgtt tattttttta aatttatggg
    37141 atgatagaaa aaaatagtag agaaaggaca cgattctact tgctattttc acttaatacc
    37201 cgtatattac gaatgaaaaa agtttatgaa aagttggaat attgaatgga aaaacaaaaa
    37261 aggttaaaaa acagccgcta catcactggg tttgatgggc tccgttcatt agctgtggtg
    37321 ggtgttatct tctatcacct tctgccaact agtatgaaag gcggctattt gggcgttccg
    37381 atttttttcg tcgtatctgg ttatttgatc acggatctgc tgcggcagga atgggaacaa
    37441 aatggaaaaa tcaatatttg gcaattttat gtcagaagaa tgaagagact ctatccggca
    37501 cttgttttta tgcttgtgac agcttcggct tatatcactt tgtttcagcg agggctgtta
    37561 aacaatcttc gaggagttgt ggtcagcagc ttactgtatg tcaacaactg gtggcaaatc
    37621 aataacggtt tatcttattt cgatcgattt gctaatgagt caccgtttac gcatatctgg
    37681 tctttagcag tagaaggtca aaactatttg gtttggccga tcttgtttgt attgttgatg
    37741 gtatttgttc gtaaaagaaa atggatcttt tatactgtgt taggtacatc cttcctctcc
    37801 gccatcttga tgatgatctt gtatacacca ggaggcgatc caacccgtgt ttattacggt
    37861 accgacactc gtctgttttc tatatggttg ggaagtgcgc ttgcgtttgt ttggccaagt
    37921 acgcgtttaa agaaaaacat cccgaaacaa gcgaaaaata ttctgaatat cgctggagga
    37981 atctcgcttt tggcactgat tctttgcttc ttctttttag atgatcatta caatttcatc
    38041 tattacggag gaatgctcct agtgagtctg ttatgcgtag tgctagttgc agtgacagca
    38101 caccccggag caagtttgaa tcgatggctg acaaatccag tatttacttg gattggcaaa
    38161 cgaagctatg gcatttatct gtatcagttt ccagttatga ttttttatga agcaaaggtg
    38221 actgatattg ccgatcatgt tttgttgcat actttagtgg aaatcacatt gattctaatc
    38281 atcagtgaac tttcttatcg atatgtggag cgtccgctag cacgttttga ttatggtcgt
    38341 acattccaag tgatgaaagg atggttcagc aaaccgatca tcagtaagag caaaccatgg
    38401 gttgttccag gcacattact tgtagcaatt gcactcgttg gtttcgttac ggcaccgaaa
    38461 aacgctgtaa cagccgatca aaaacaactg caggcaaaaa tcgctgaaag taaaaaatta
    38521 gcagaagaaa ccagaaacaa ttcagaaaac accactagtt cggttgacca agcgatcctt
    38581 gataagtacg agttgtccaa ggcacaaggg aataaggcac aaaaattaga actaacggca
    38641 tttggtgatt cagttatgtt ggatgctgca acggatctac aagaacttta tccaaaagta
    38701 gtggtagatg gagatgtcgg acgacaatta tatgccagtg taccgtacat tgaaaaatta
    38761 aaggaacaag atttgttgaa agacactgtt ctgattggac taggaacgaa tggctcattc
    38821 tcggaagctc agtttgatag tgtgatggat gcaatcggtg atcgaaaagt atactgggtc
    38881 aatgtccgag taccgacgaa gcgctggcaa aatgacgtga atgcgatgct tgaacagatg
    38941 acgaaaaaat acgacaatat gacgctgatt gattggtaca gctacagtaa tgaccatagt
    39001 gactggtttt atgatgatca agtccatcca aatcctgaag ggatgacaca ttacattcac
    39061 ttagtcagcg aagccttgct aggaggaagt tcaagtacga acagcagtga gacaaacgtg
    39121 acagaatgat agctacaaaa aacttcaggc aaaaacattt gtttttgcct gaagtttttt
    39181 tatatttacc gtgtttttaa cattcatata gattcaggaa gcaaaacggt tgagtgtgtc
    39241 tgtaaataac gttctttttg ttttcgggtg agttttttga atgcagcttc tgcttttgtt
    39301 gcttcgcttc ttgaagaaaa tacttctgca tggatcatct taacaggtct tcttttttga
    39361 agacgagtat atttagcacc agtaccattg ttatgttcct ctaatcgtct agaaagatcg
    39421 gttgtatatc caccataaaa tgtctgatct ttacagaata atacgtaaaa ataatgttca
    39481 tttgccatag agcatctcac gaatctctgc agtgtattct ccatccatat tgtaagtgat
    39541 caatggtgga gcgactttga atccgtctaa tttgccttct ttgattcctt caactaacaa
    39601 aatatttgcc tcttttccag gttttgggta aacaaattgg atccttttag gtgcaattcg
    39661 atagtcttcc atgacctgaa tgatttctaa gaaccgatca ggacgatgga ccatagtaaa
    39721 gcgtccattt gtttttagta gcttacttga aatatcgatc acttcttcta acgtcgtatg
    39781 aatctcatga cgggcgattg ccaggtgttt attagggttc gttttatttg ttggcagccc
    39841 tttgaaataa ggtggattac agacgactaa gtcacaagaa ttatgtcgaa cgacagataa
    39901 gctgtctttc agatcgatgg tgtggacagt catttgttca tttaaatggt taagcgcgat
    39961 actacgtctt gccatgtctg ctagacgagg ttgaagttcg atagcgtcga tagccgcctg
    40021 ggttcgttta cttaaaaaaa gtccaactgc gccatttcct gcacaaaggt caacaatctt
    40081 ccctttttta ggtacacggg aaaagtgagc aagcagcaca gcatctaaag aaaaagaaaa
    40141 cactttggaa ctttggataa tcttgatatc ttctgcgtat aattggtcga ttcgttcgtc
    40201 ttcgtgaagc atggattccc tccaaaatga aaaaaatagt cgtctgtatt atactatttt
    40261 tattaaaaag aaccaaggtg gcttgtcaaa cctctatttt ttatgcatac tatgaccata
    40321 agaagggaaa ggaagagact atgttttatc gatttatgcg cggggtagtt cgagtactat
    40381 tagccttagt taatggaaat gcccgctatg agaataaaga agttttaccg caagatgaga
    40441 attacatact agttgctccc catcgtactt ggtgggaccc attatatctg gcggtagctg
    40501 cagcacccaa ggaattcagc tttatggcaa aagaagaact gttcaaaaat ccagttctgc
    40561 gatttatatt aactcgatcc aatgcttttc cagttaaaag agaaaatcca ggaccaagcg
    40621 tcattaaaac acctgtaaag attttgaaat cgacaaattt gagtttgatc atgtttccca
    40681 gtgggacaag acatgctaca gaacttaaag gcggaatggc attgatttcc agaatgtcta
    40741 aagcgaaaat cgtgccagct gtatatcaag gtccgttgac acttggggat ctgttcaagc
    40801 gtcagagagt gacagtgcgt ttcggcgaac caatcgactt atcagatatc aagaaaacgg
    40861 ataaagaagg tatggcggag atcgaacgac gaacacaagc agcttttgac aaattggaca
    40921 aagaagtgaa ccctgatttc aaatatgaag tcaaaaaaaa ataaaatgat aagtttatgc
    40981 gtgggcggtc ttttgaatct actgtcctgt tcagttcaga ctttttcaat gaaaacgagg
    41041 gagagacgtc aagtctgccc tctttttgcg ttccaatatg taaaatagaa gacaggtgtc
    41101 atgtaatgga tcaaagtgtt ataataatta gaaggacatt ttttgaggtg attgaatgcg
    41161 ttttttacat acagccgatt ggcatatcgg aaaaaaacta caaggttatg acttgattga
    41221 agaccagcgt catgtactat cttctatact agatatcgcg aaagaagaaa aagtagaggc
    41281 aatcgtgatt gccggagact tatatgatag gagtgtccca gctgttgatg ccgtagaatt
    41341 atacaatgaa ttgatggtcg attggaattt aaaggaaaag atcccgattt ttgctatatc
    41401 aggaaatcac gatagcagta cgagactttc agcgggtacg ccatggttca gttattcgaa
    41461 ctattttttg cgaacacagc tagcagaagc atttgaacca gtcgagttta aaaacgtaca
    41521 atttttcttt cttccttact ttgaaccaat tgcagcgaga ttatattttg aagatgacaa
    41581 tatccgaacg atcaagcaag cggtagccaa ggtgatagat aaaatgcagg agctatttga
    41641 ttcagaaaaa aaacaagtat tgattagcca tttttttgta atgggaagtg taaaatctga
    41701 ttcggaaacg aaaatcgaag taggcgggtt ggattcgatc cctggtgaac tacttacacc
    41761 gtttgattat gtagcactag gacatttgca taatcaagct gctttgagac aggataatgc
    41821 tcgctatagc ggttccccat tgaaattctc tttatctgag atcaaccaaa aaaaaggcgt
    41881 atggatcgtt gacctgacaa caggcctgga gttgaatttt agggagatcg aacccctgca
    41941 tgatattgaa gaaataacag cgagtttcaa agaattgctg gatcctgcct tttatcagtc
    42001 cgtcaatcgg gagaattaca tctatatcta tctcactgat agaacgatca tcccgaatat
    42061 gatgaaccag cttcggcaaa tatatcctcg tattttgggg attgaacggg caaacggtcg
    42121 agcagaacaa acaaagcgca aacgaaaagt actagaaatg aaagatcctc aaaaattagc
    42181 agaagcattt tttagtgaaa tgacggaaga gcagctaact gcaaaccaag aaaaatggct
    42241 cggagaaacg ttgcaagaaa tcatggaaat ggaatgatca agaatgaaac cgaaaaaatt
    42301 acaacttaaa aattttggtc cttttatcaa tgagaccgta gatttttcac gtttgacaga
    42361 agcaccgctt tttttgatca gcgggaagac aggagctggc aaaacgacga tttttgatgg
    42421 gatcaccttc gcactttttg gtgaaacttc cggaagactt cgctctggaa aagagatgcg
    42481 ttctttgttt gctacaccag acgaagaaac aagcgtgact ttttcttttg aacatcagca
    42541 gatgagctat gaaatcgaac gaaaaccaga acaagtttta gcaaaacgaa aaggaaacgg
    42601 aacaaaaaaa caggcagcca aagtaacgtt gaccattttt gatgggaaag gaaaagaaat
    42661 aaaacaaatc acaaaacgta cagaagcaga tcagttgatc agagaactga tgaatctcga
    42721 tgcgaaacag ttttctcaaa tcgttttact accccaagga gaattccgta attttttggt
    42781 ttcatcaagc agtgataaag agactgtttt acgtcattta ttcggtactc aatttttcca
    42841 acagttcaat gagcgattaa aagaaaaagc aaaacaacag caacaaaaat tggatcattt
    42901 agagcaagag ctgcatcttc tgcaaaaaag gtttgtttta gtcaaagaag aggaaccagc
    42961 agcagggttt gaagccgttt tagcccaatg gacaaaccat caggcaatac tcgaaaaaca
    43021 aatcacggaa gaaaaagaag ctctaacgga acttcaggaa aaacaaaagc aactagaaaa
    43081 ttcctactat cgttttgaaa gcttgaaaaa aagttatgaa gaaaaaacga tattaataaa
    43141 caagcaaaag gaattagctg ctcaaaaaga cgaaatagag gaaaagaaac gatggattgg
    43201 gtattatgaa tttacgcaac gattagtcga accaatccag cgcctacaag aaataaaagt
    43261 tgaaaaaata gaactgtcac aaaaaagtag tgagcaaaca aagaaactag cgatgactac
    43321 gcagaattat gaagagtggc aaaaaaatga agcaaaccgc gtacgccgac aaacaaaaat
    43381 caaagaagct gctcaaaaac tgcaaaacaa acagatgatg gtgccaattg tcgaatcctt
    43441 acgtagtaaa aaacaagacg cagaacaatt aacaatttca ttggataaag aagaattgaa
    43501 tgccgagaag ctgtatcaaa atctcgctat ctatcaggaa agaactcgaa ttcttcaaga
    43561 aaatttactg gatcaggaaa aactgcaaga agatcgtctt gcttttggac agttaaaata
    43621 tctagatgaa aagcagcaag aaatagaaaa acaaaaagca aaagacactg ggtcgctcgc
    43681 acagataaga gaagaactca aaaaagcaac tgaagaaata acaaaaaata gtcagctatt
    43741 gaaagaacag acagaacaag cagaacagct gaaaagccaa tgggcaaagc agcaaattgc
    43801 tcgtcttcag ctttttttgc gtccagggga gccatgtctt gtttgtggat caacggaaca
    43861 ccccatacaa caattaaaac acgaacagcc aaataaggaa gatatcttaa aagtagaaca
    43921 acaactggca gaagctgagt ctgctgtaga aagaactcag caaatcattg ccaaaagtga
    43981 agcacatgaa aataatcttc aacaacaatt atcagattta gaacaaaaaa tcatggcaac
    44041 gaatatacaa ttggctgaac aagaagaaca cttgcaacag gctttgaaaa aggcatttcc
    44101 tgagataaca gaacaaaaaa caaaaacaac tcttgtagta atcgaacaat ctttagatag
    44161 acgaaaaagc aagatggcca atgaaagaat agagctggaa gaaataagac aacaagcaga
    44221 aaagctcgaa caacaattag ctgtctacaa acagcagcag acagaaaaaa ataatcagct
    44281 ccagcaactg caaggagaaa tcaaaagttt agaacaacaa ttgcaagata cggatagtaa
    44341 ctgtttagaa gaggagatac gacgacttga aaaacagtta gaagaagact cgaatcaact
    44401 agctgttgag cagaaagcag gagtgcaatt gttgcaagat cttcatacgc ttcggacaca
    44461 gcttgatctt caacatgaac aaatagaaaa aatagatgaa aaaatagaaa aggaacaaga
    44521 aagaatagct aaaaagttag aaagccagtc gtatttttca acaattgaag aaatctgtgc
    44581 atttgcacaa cagcaagcac aatataaaga aaaccaacgt acagttactt cttatcatga
    44641 agagcaatta gtgacagctg accggctgcg tcaattagaa aatatccagc taactgagac
    44701 attcccagat acagaagaga tcagccaaca gttagaagaa ataagacgta tgatcgaaat
    44761 aaagcaagaa aagatatacg gtttacaaga acaaaaaatt agtaatcaaa aaatctacga
    44821 tgaatgtctt tcgatttatc acacaagcca gacgaaatta gatgagctta cgcaattaca
    44881 gcagctttcg caaacattca acggagaaaa tcctaagaag actagtttgg aacggtatgt
    44941 gcttcaggtt tatcttcaag aagtgttaca agtagcaaat gatcatctcc aacgtttaac
    45001 gaaaaatcgt tatcaatttg agctagcgga taccattggc agttatcgag ggaaaaccgg
    45061 attagaaatc aatatctatg atgatcaggc aggtatgtct cgaagtgcgc ataccctgtc
    45121 tggcggtgag agttttattg cagcactttc tttagcatta gctcttgccg atgtgatcca
    45181 gacacaagct ggtggtgtag caatcgatgc attgtttatt gatgaaggat ttgggtcatt
    45241 agatgaagaa gctttggaaa tggcaatgga agctttagaa acgatcgaaa gtgaaggaag
    45301 aatgatcggc atcattagtc atgtaagaga attgaaagaa cgagtattac aacaaataag
    45361 aattgataca gaaggtagcg gacaaagcaa aatacgctat caacttggat aaaaagggag
    45421 attggtaact atgaaatgga gtattccaga aaaagtcatt gcacgtggac gtcaatactt
    45481 gcaagaaggc cgagtgttat cggttgtccc tgaccaagaa gaaaacgtgt ggcatgcaga
    45541 agtcttgggc agtgaattgt atatggttga tttggatggt acagcaaaag agatagatta
    45601 ctgccaatgt ccctattggg aggaacataa atattgtaag catacagttg ctgtggaact
    45661 ttatttacgg tcgaaaaatc tatctcgtat catgaaaaaa gaccagccaa tgctagaacc
    45721 ggtaaaagca acaagtgagg gagaaatcat tgcaaaaggt ttttctcgtc taaaaacaca
    45781 aaacaatcct caagcggtgc aacctttgat tattgaatac caagtagaaa cgattgagac
    45841 taatcagtat cacccagagc tttctatttt ggcactttac ttgcgtatcg gttcattggc
    45901 atcaccgaaa aaaacatata tcgtaaaaaa catctatgat tttttacaag cttacacaga
    45961 aaaagaaact tacactgtta ataaacagta ccagtttcgt ctagataaac gggcgtttca
    46021 aaaagaagat caaaaaatac tcacgcatct tgcaggaagt gctcagacgc atcaattatt
    46081 aggtacgaac ggcttacaag tcaaaggaaa actagataaa agatatctgc ttctcccagt
    46141 agaacaagcc cgcttcctgt tagagcagat gaatcagact acacgtttgc atctgcagat
    46201 aaatgaacaa aacgatcaag gaatacaatt ttctgatcaa aacaaacctt tatcattcaa
    46261 agtggaaaaa tttggagaag attatttgct gcaagcattg aatgattttg attttttctt
    46321 tatgcattat cagtggggag ttatccaagg gacattttat tctctgacga aatatcaaca
    46381 aacggtttat caaacttgga aacaattgat tcgccgattg gagaaaccag aagtagtgtt
    46441 caagaaaaaa gaattaccgt ctttgtttaa agagattatc cctctgctgt cagagatcgg
    46501 cgacgtagct attgcccctg cagtagaaga ggaagtagca gatatccccg tcgaatttat
    46561 tttctttttc cgaaaagcaa aaggaaaaat ccaagctcga gtcgatttcg tctatggtga
    46621 agtcatctat tcgactgacc cgaagcatga ggtctcgaca gaacaggctt ttcaggtgtt
    46681 acgtgacaca gtagaagaga aacgagtact tgatctattc agaatgtatc gctaccagaa
    46741 aaatgaaact ggttatgaac gtccattgcc aagcggggaa gaactatatg cctttttccg
    46801 cacagaacta tctgtcttcc gtcagtttgg tgaagtcaga cttggaagaa aattgcgaga
    46861 gttgtattta gatgcgcata aacatcaacc gaaaatcgaa gtaaccgaga ccggttcatg
    46921 gctcgatatt cgattcgatg tgacagggat agaggagcaa gaaatcgatc atgtcctgca
    46981 aagcttgctg agaaatgatg ctttttatac attagaaaat ggtcaggttc tttcttttga
    47041 ttcagaagaa tttcaacaaa caagccaagt gttgcaacaa ttacgggaat cgatacgtac
    47101 agaagaagga acgatccatg tacctaaaaa tcaaggattg atcattcaaa atcaattgga
    47161 aaaaagcaat gctactttta gtgaatcgtt ccaaacgatg gtacaagatc tcattcatcc
    47221 agaaagatac caggcacagc ttcccaaagg attgaatgca acgatgagag attatcaaaa
    47281 gcaaggtttt cgatggttga agatgttagg tcattatcag tttggcggta ttttagccga
    47341 tgaaatggga cttgggaaaa cactacagac aattgccttt ttactttcgg aaaaagaaga
    47401 acggaaaagc ttctctgctt tgatcgtagc gcctgcgagc ttaatttaca actggcaggc
    47461 agaggtgaga aaattcgccc cttctttaag tatccaagta atcaatggga ataaaaaaga
    47521 acgtgaagaa ttactggcta aagacacaga tatccgagtg acttcttatg ccagcttaag
    47581 acaggatcta gctaattatc agtcgcaaaa aattgattat ttgattcttg atgaggcaca
    47641 aatggtcaaa aatagcagta cgaaaactgc acaagcattg agagaattag ctgtacctca
    47701 gcggtttgca ctgagtggga caccaatcga gaataattta gaagaattgt ggtcattgtt
    47761 tgcaacgatt atgccaggat ttttcccgac taaaacaaaa ttcagggaac ttgccgcaga
    47821 agaaattgcg caaatgattc gaccgtttat tttacggcga gataaacaaa ctgtgttgaa
    47881 agacttgccg gaaaaaacag agatgaatct atattctgca cttacagaag aacaaaaaac
    47941 tgtttattta gcttacttgc gtcaaatgcg tgaagaaatc acatcaatgg attctgaagc
    48001 attcaagaaa aaccggatcg ggatacttgc tggtctgaca agactgcgac aaatttgctg
    48061 cgatcctcgt ctgtttattg aagattacca aggcggttcc ggcaagctcg aacaagtaaa
    48121 agatctactg gtagctgcca aagagaacaa acgaagagtt ttgctgtttt ctcaatttac
    48181 agggatgctg actatcttgc aagaagagtt agccgagcta gggatctcga cattttattt
    48241 aagaggcagt acgaaaccac aagatcggtt aagtatggta gacgcattta atgctggcga
    48301 aaaagatgtt tttctgattt ccttaaaggc tgggggaact ggattgaatc tcacgggagc
    48361 agatacggtc atcttgtatg acctttggtg gaatcctgcc attgaagaac aagctgctgg
    48421 acgtgcacat cggatcggac aaaaaaatgt ggtagaagtg tggcgtatga tctctgaagg
    48481 aacgattgag gaacgcatgg attcactcca gcaagaaaaa cgcgaactgt tccaaaaagt
    48541 gatccaaggt aatgaagaac agttgacgaa actgactgaa gaagacatcc gcatgatttt
    48601 aagtgtagga gaaatcgagg agtaagagac tcgaaaaagc gttcagtaat gggtaagaaa
    48661 gcctttctaa agaagcgatt gtttatttga tttaaaaaat agcaaggagg ctgggattcc
    48721 attttatccc agcttccttg ctatcatttt tttatttcta tttttaaagg ttatacaaag
    48781 attgtcttca ttgtctctaa cagctgatca aaagaagaaa tctcgatatc gcaaattgga
    48841 gtctgttcaa ttttgcgttc tcgatggttg aaccaaagcg cttgccagtt agcacttttt
    48901 gcacctagaa catcattgtc gtaattatcg ccaacgtata aggtctcttc tggaagcata
    48961 tgaaattctt tctcagccag ctgaaaaatc tctttttctg gtttttggaa acctgttgct
    49021 tgagaaatga gcatattgcg ggaaggaatc caattgttca attgaagttg cttcagtttt
    49081 tttgtctgat ggtcagtcgg cccatttgtg ataattccta atgggacatt cttttccttc
    49141 aagaaatcga gggttttttt gacctcctca tgcaagcaga tattatcaag ttcttcctcg
    49201 taaatttttt gaaataagag gccatcttct tccgtaatat gagggtagtc taaatctttt
    49261 agtgattggc tgatccgatg tgctcgcata tattctagtg tccaatctcc tgccattact
    49321 tttgggaagt tttcatcact atgatggcgg aaccggatat atagtggatg catatctgca
    49381 tctgaaacaa gcggaacgac tcttttgacc gcgtttcgaa atggctgctg ctgatcatac
    49441 atcgtatcat caacatcaaa cacaactgat ttaatcaaaa ttttacacgc ctttcggttt
    49501 tcattgattt tttcacaaat ccttgactat tgtaacagaa aaaattgtct aaaaaagctg
    49561 ataaataaag gtttttctta ctatatttct atttccttga cagaataagg tttttatatt
    49621 tcagtttttt tcactaaaaa tcgtgaaaca cgtcaataag tttgatgaac aaattaatca
    49681 ttatggtaga agtcgattga tttattttaa tgcaatctct gtggaatttt cgaaagattt
    49741 a
//
LOCUS       DAYYCY010000004        44471 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000004 DAYYCY010000000
VERSION     DAYYCY010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44471
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.004"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(38..1189)
                     /locus_tag="ACHAZB_000182"
     CDS             complement(38..1189)
                     /locus_tag="ACHAZB_000182"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFM8612896.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(1202..1639)
                     /locus_tag="ACHAZB_000183"
     CDS             complement(1202..1639)
                     /locus_tag="ACHAZB_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8612897.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            complement(1654..3420)
                     /locus_tag="ACHAZB_000184"
     CDS             complement(1654..3420)
                     /locus_tag="ACHAZB_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol-specific transporter subunit IIBC"
                     /protein_id="HFM8612898.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            3684..4361
                     /locus_tag="ACHAZB_000185"
     CDS             3684..4361
                     /locus_tag="ACHAZB_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFM8612899.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            4364..5491
                     /locus_tag="ACHAZB_000186"
     CDS             4364..5491
                     /locus_tag="ACHAZB_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8612900.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(5691..6131)
                     /locus_tag="ACHAZB_000187"
     CDS             complement(5691..6131)
                     /locus_tag="ACHAZB_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8612901.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(6142..8175)
                     /locus_tag="ACHAZB_000188"
     CDS             complement(6142..8175)
                     /locus_tag="ACHAZB_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HFM8612902.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            complement(8177..9880)
                     /locus_tag="ACHAZB_000189"
     CDS             complement(8177..9880)
                     /locus_tag="ACHAZB_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8612903.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            complement(9974..10924)
                     /gene="manA"
                     /locus_tag="ACHAZB_000190"
     CDS             complement(9974..10924)
                     /gene="manA"
                     /locus_tag="ACHAZB_000190"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HFM8612904.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            complement(10963..11988)
                     /locus_tag="ACHAZB_000191"
     CDS             complement(10963..11988)
                     /locus_tag="ACHAZB_000191"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFM8612905.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            complement(12197..13066)
                     /locus_tag="ACHAZB_000192"
     CDS             complement(12197..13066)
                     /locus_tag="ACHAZB_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8612906.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(13525..15417)
                     /locus_tag="ACHAZB_000193"
     CDS             complement(13525..15417)
                     /locus_tag="ACHAZB_000193"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8612907.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(15414..17141)
                     /locus_tag="ACHAZB_000194"
     CDS             complement(15414..17141)
                     /locus_tag="ACHAZB_000194"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8612908.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(17157..17807)
                     /locus_tag="ACHAZB_000195"
     CDS             complement(17157..17807)
                     /locus_tag="ACHAZB_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HFM8612909.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            18011..18307
                     /locus_tag="ACHAZB_000196"
     CDS             18011..18307
                     /locus_tag="ACHAZB_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFM8612910.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            18479..19099
                     /locus_tag="ACHAZB_000197"
     CDS             18479..19099
                     /locus_tag="ACHAZB_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFM8612911.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(19191..20357)
                     /locus_tag="ACHAZB_000198"
     CDS             complement(19191..20357)
                     /locus_tag="ACHAZB_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM8612912.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(20369..20722)
                     /locus_tag="ACHAZB_000199"
     CDS             complement(20369..20722)
                     /locus_tag="ACHAZB_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFM8612913.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            20850..21872
                     /locus_tag="ACHAZB_000200"
     CDS             20850..21872
                     /locus_tag="ACHAZB_000200"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HFM8612914.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            21891..22052
                     /locus_tag="ACHAZB_000201"
     CDS             21891..22052
                     /locus_tag="ACHAZB_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8612915.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(22311..22745)
                     /locus_tag="ACHAZB_000202"
     CDS             complement(22311..22745)
                     /locus_tag="ACHAZB_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8612916.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(22855..24714)
                     /locus_tag="ACHAZB_000203"
     CDS             complement(22855..24714)
                     /locus_tag="ACHAZB_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFM8612917.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            24715..24855
                     /locus_tag="ACHAZB_000204"
     CDS             24715..24855
                     /locus_tag="ACHAZB_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HFM8612918.1"
                     /translation="MLAPSFLSIKECCFSYFSCTKFESKERKEELMKSKYSLSLWPFW
                     FL"
     gene            complement(24832..25992)
                     /locus_tag="ACHAZB_000205"
     CDS             complement(24832..25992)
                     /locus_tag="ACHAZB_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFM8612919.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(26299..27723)
                     /gene="araA"
                     /locus_tag="ACHAZB_000206"
     CDS             complement(26299..27723)
                     /gene="araA"
                     /locus_tag="ACHAZB_000206"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFM8612920.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(27739..28437)
                     /locus_tag="ACHAZB_000207"
     CDS             complement(27739..28437)
                     /locus_tag="ACHAZB_000207"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFM8612921.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(28394..30055)
                     /locus_tag="ACHAZB_000208"
     CDS             complement(28394..30055)
                     /locus_tag="ACHAZB_000208"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0005998 - xylulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFM8612922.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            30273..31673
                     /locus_tag="ACHAZB_000209"
     CDS             30273..31673
                     /locus_tag="ACHAZB_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HFM8612923.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(31925..33004)
                     /locus_tag="ACHAZB_000210"
     CDS             complement(31925..33004)
                     /locus_tag="ACHAZB_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8612924.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(33090..34049)
                     /locus_tag="ACHAZB_000211"
     CDS             complement(33090..34049)
                     /locus_tag="ACHAZB_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFM8612925.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(34061..35572)
                     /locus_tag="ACHAZB_000212"
     CDS             complement(34061..35572)
                     /locus_tag="ACHAZB_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HFM8612926.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(35594..36157)
                     /locus_tag="ACHAZB_000213"
     CDS             complement(35594..36157)
                     /locus_tag="ACHAZB_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFM8612927.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(36163..37026)
                     /locus_tag="ACHAZB_000214"
     CDS             complement(36163..37026)
                     /locus_tag="ACHAZB_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8612928.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(37040..37936)
                     /locus_tag="ACHAZB_000215"
     CDS             complement(37040..37936)
                     /locus_tag="ACHAZB_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8612929.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(38111..39400)
                     /locus_tag="ACHAZB_000216"
     CDS             complement(38111..39400)
                     /locus_tag="ACHAZB_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8612930.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(39372..40889)
                     /locus_tag="ACHAZB_000217"
     CDS             complement(39372..40889)
                     /locus_tag="ACHAZB_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HFM8612931.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            41087..41977
                     /locus_tag="ACHAZB_000218"
     CDS             41087..41977
                     /locus_tag="ACHAZB_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFM8612932.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(42050..42874)
                     /gene="nadE"
                     /locus_tag="ACHAZB_000219"
     CDS             complement(42050..42874)
                     /gene="nadE"
                     /locus_tag="ACHAZB_000219"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFM8612933.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(42886..44358)
                     /locus_tag="ACHAZB_000220"
     CDS             complement(42886..44358)
                     /locus_tag="ACHAZB_000220"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFM8612934.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
BASE COUNT        12817 a         9308 c         7715 g        14631 t
ORIGIN      
        1 aaaaatttcg tcacattccc ctttttcgta tcctatttta agcgacttgc ttggcataac
       61 ggttcacgtt ttgcttgata ttcccgattg ttttcggatc attgattcct gtgacttctt
      121 gaatcaattg ttccaagttt tcatggatct tcatttcatg caattgacgg ctttgttcat
      181 cttcaggatc atagtaatta aaaataatcc ccattgttgc cgtcaaatgt gggcaagtca
      241 agttatgttc ttgtagttct ctcactggac gtgtaaatct ttcttgataa cctaacttac
      301 gtaatggtgt ccgagcgaca cgtgtgatcg catcagagat atttttgttt tggaagcgat
      361 gaatgatttt ttcaatatac gcatgatgct gctctgcatc aaacccccat ttggcaatca
      421 gcaaacttcc tgtttcctgc aaaactgatt ttaattgtgc cacaactaac gcatcttgca
      481 ttgcttcatc aatggtctgg taacctaaca aggcacctgt ataagcgaca gtcgcatgac
      541 cagtattgac actaaatagt ttgcgttcga tatatggttc tagatcatct acatataaaa
      601 ccccttttaa ttggattttt ttattttttc tttggctatc gtcaatgacc cattctttga
      661 aaggttccac ttggacgaat aaaggatctt catggtgttg taatgggacg attcgatcga
      721 ctgctgcatt tggaaagccg atatatttgt cagtaaatga tgtttccgga agatatttat
      781 acacttcttt ttctagaaat gctgaaccgc caatcatatt ttcacaggca atgatatcta
      841 aagatgttgt gcttccttcc ttttctcttt cttggatacc ttttgcaatc aactcagcaa
      901 taaaaggtaa gatattcggg ccaatggcgg tagtaactaa atctgcttca gcgatttctt
      961 ggattacttt ttctggattt gtttgattgt tgattccttt gacatttttt acagtgattt
     1021 tttcttttga ttcatctgct aattcgattt cgtattcttt tctttcattt aaagcatcaa
     1081 tgatcgtgtg attcacatct acgaaagtaa tctcaaatcc attagctgcc aaaacctctc
     1141 caataaatcc tcgaccgata tttcctgcac caaaatgtac tgctctcatt tttctctctc
     1201 cttaagcaat tgctagattt tccgttactt cttctttgct gagcgcgtct gctagctgga
     1261 caacattgtc catatcactg caataaagtg cgatttgctg tacaagttgc aaatgctcat
     1321 ctccgactcc tgcaataccg aacaaaacag tcgcaatctt ttcttcttct tcggtaccaa
     1381 aatctacacc atctggtact tgaacgacac agatgccgga ttttttcaca tatttttttg
     1441 cttcatctgt tccatgagga atcgcgataa aattccccat ataaacagat aacattttgt
     1501 ctctatctac catggcatcg atatattcct catcaacgca ccctgcttct acaagcatct
     1561 gaccgcaata gcggatcgct tcttctttgg ttttaaaaga tcgattgagc tcgatcatgt
     1621 cgaatggcaa actttgcatg ctgttttcct cccttaagct gtctgtttca aatattgtgc
     1681 aatcgattcg tatttttgcg gattgaccat gctgttgact gataaatgaa tggattgagg
     1741 tgcagccatc tgtacttttt ccgctaaact ttctttggct acgatcagtg aatcaggttc
     1801 atcttttaaa tcttggatat tgacttttct taacgggata gttaaatggt tcttgtctaa
     1861 tatatctttg agcacggtca tccccattgt tgctgatccg gaacgatcat cgtggacaag
     1921 tagaatctct ttgatttgtt tgatgtttgc tgaaagaaac tcttctgctg gctcattctc
     1981 aatttcagaa actgatgttt tctgcttgct gcttaattct tgaatgattt catcatatcg
     2041 aggtgaattc aaaaagtttt ctactgagac aaaacgcgcg ctcggtgctt tttgttttgc
     2101 tctttcttgt agttcgacct gagtaacgat caatgtattc gagtcatctg tcagttggct
     2161 gattgcttga ttggtcaccg tttgaggcaa gtctgcttcc tgtacttttt ttctcaagat
     2221 cgaagcgccc atcgcacttg atcccattcc tgcatcacaa gcaaaaatga ttttttgtac
     2281 gccagagaat gtagctcctt gaggaatacc ttgggcacct ttggaagctt gcttagctgc
     2341 ctttgtctct tgaacagatt tttcgaagtc ttcatttgta tctttcgcat ccgctttcaa
     2401 aattactgca gaaataccaa acgaaacagc tgcggcaaca atcacgccca aaataacagg
     2461 aaggtaagaa ttgaacggtg tcatcgctag aatcgcaatg atcgaccctg gagaagctgg
     2521 cgctcttagt ccagcatcca tcaattggaa aatgaaactt cctgacattc ctccgcctat
     2581 aactgataag aataataacg gtttcatcat aacgtaagga aaatagattt catggatccc
     2641 gcctaaaaag tgaatgatca tggctcctgg tgctgaagat tttgctgctc ctttgccaaa
     2701 caaagtaaaa gcaagtaata cgccaagtcc tggaccagga tttgcttcaa gcaagaatag
     2761 aatagatttt ccagcctctg ctacttgttc agttcccaat ggtgtcaaaa tcccatgatt
     2821 gattgcatta ttcaaaaata agatttttgc tggttcaata aaaatacttg tcaatggaat
     2881 caaatgggca gataaaatga tctctacacc ttttgccatt gcttgagtca atgcatcgac
     2941 aactggacca atcgcagaaa aacctaataa cgctaatcca aaaccgataa gacctgctga
     3001 aaaattattg actagcattt caaaaccgga tttgatccgt ttttggaata gctgatcaaa
     3061 tttcttgatg gcatatccac caagaggtcc catgatcatc gcgcctagca tcattggaat
     3121 atctgttccg acaatcacac ccatcgttgc aattgcccca acaactgatc cgcggtcacc
     3181 agcaattact tttccgccag tataaccgat caataaagga atcagataag ttagcattgg
     3241 gcttaccatg gtagccaaag cctcatttgg taaataacca tctgggataa atagagccgt
     3301 taatacaccc caggcaatca gtgctccaat atttggcatg accatgcttg ataaagtact
     3361 gcctaatttt tgtaccctcg cttttagcga aatgttgttt ttgggaatcg tccgttccat
     3421 tttgtctctc cctctctcct tttgatactc taattgtatc cgcttactaa aaagagaaat
     3481 agtcgtactt tggcacgaaa ctttgtgcca aaggtttgag tatgtcagca aaataaaaag
     3541 atttcggtat cagaaaactt caattttaaa taaaactgtt attttaattt ttttataata
     3601 tgcaaacaat aacttttgtt agcatttaat ttagatgttt tccattctat tatgttatta
     3661 aattttatat tggaggccta tctatgacat tactgatcaa aaacatgaac atgaaacgtg
     3721 gtaaaaaacg tgtgattcga caattgaatc ttttagtaga atcaggagaa gtagctgctt
     3781 tgattgcccc gaacggattt ggcaaaacaa ctttgctgaa tggcattgcc caactgctgc
     3841 caacaactta tgatcaattg tcccttcaca acatctctcc tactgcttat acagattatc
     3901 gccggtcttt cttttttcta gaatcaacga atcagttatc cgactatcta tcggctgttg
     3961 attatttgac tttgatcaaa tactattggc agagcaaagt agaatgccaa actgttttcc
     4021 aacgattgaa gatgactgag taccttactt tgccgattgg aaaaatgtct ctaggaatga
     4081 aacaacatgt cttgcttgct gcttacttga tcagtgattc atctgtcatg ttattcgatg
     4141 aaccgttgaa cggattagac ccaggaagta ttgatatctt gaatagcctc atcagacaat
     4201 ggcagaaaga ggggaagacg attcttcttt cttcccacgc tatcgcgaat atccaatcga
     4261 tttgttctaa agcatgtttt ctaaaaaatg gagaaatcgt cacagaatcc tcaaatatgg
     4321 aagagatttt agctatttat cgtaaattat atgaggtgta actatgtctt tacttacatt
     4381 tgaacgcaat ctcttactaa aaaacaaagt aaacttttta tttcctctat tgttggtcgt
     4441 gctctttgca ttcccacttt tttttgatca taaacttgct tatacagaat tcgatgaact
     4501 gaatcataac tatgaagaga tgcaacgttt gattgaaacg ttgaaagaag atgagaatga
     4561 gaaagaattt gtggaaagtt tagagaaaag caacaaactg atcgaagcaa tcttacatgc
     4621 aaaaaacaca gggaatgtcc agcaaatggt cgaagcaact tatcattatg aaaaagatat
     4681 actggatcga ctgatttctg gtcaaagaca aggaattcca atcattgaac aacaaaaacg
     4741 tgtcgagttg ctccgttata tgaaagaaca tcagatccaa agatattcta tttttgactt
     4801 gccagcgcat ctccctttag caaattacta cgaaaatatt ttttcaggaa tgatttctag
     4861 ttttttgatt ttatgtataa ccgccttgtt tctttcttcc atcatctctt atgaaaaaag
     4921 aaagcaggta atctctctag tcaatctatt accggatagt atggtaaaga aacatagcat
     4981 ccgttttacc atttattatg gtgcagctat gctaagtctc gtgatgccat ttttgatcgt
     5041 ctctatacta gtaataatca aaaatggttt gggagacttc cgttatcctg taggaacgat
     5101 cattggtcag gagatacgaa tccttccgat gtatgagtat cttttccaga gctttctgtt
     5161 cctgttgttg tgggtgctct ttttaagtac aatcagtttt cttctatctg ctttatttga
     5221 acattctcta gtcaatcttt taggtacact tctttgtctt ttcttagcag agtatcgctt
     5281 gttttcttcg attggatgga tagaatcaat cagtcattac ttgccaactt cttatgttga
     5341 ttttcaaaat gtgatcatag gaggagatat tttctcgcca ctagcttcag aacaggttac
     5401 tttcatgaat ggtatcttga cacttggtat atggagtatc gttttattat tcataggtat
     5461 gggcactatc tatataaaaa agagctatta aaacacaaaa ataatagggg cggtgacaaa
     5521 aaccttgtca ccgtccccta tttcaaataa acggttttct agaataaata gttttcaaaa
     5581 gcttgcatcc gcagtaataa gaacctccca acaaaaatag ctcctcttat ttttatcttg
     5641 gttgaagtta ttacttgatg caaggcagct ttttttactg tgaacattgg ttatatatgt
     5701 ccatgttcaa aaatcatttg gacttcttca atggttttcg catcactgag cgccattact
     5761 tggtcgatgt ctgaacagaa aaaggctaaa tcttggagca ccgctaactg atgttctttt
     5821 tttaaagcca ctacaaataa gagtgtcgcg atttttggtt cctcttttgt tccaaagtcc
     5881 acaccatcag gcacttggac taaatagatt ccttcttcta ctacttcttc atctttgtca
     5941 gtcccatgtg gcaaggctac gaaattcccg atatagacac tcgctttttc ttgtctttcg
     6001 atcattgatt ggatatatgc cggtttgatt cccccttgtt gggtcatcca ctggccgatc
     6061 tcggtcaacg cttctttcca attttgatag gattgatcga gcaatatttt ttctgctggt
     6121 agctgtatca tcttttcacc ttcatttctt tatttcttca atcaggattt tcgctaacaa
     6181 ctgataaacg atttctttat ttcctgagcg gaagatctcg gtgttcaagt cattcatgat
     6241 gattgctcca ctgatctttc ctaaaagaat cccgtctttt tcttggattg gcgaaggcgc
     6301 cagcataact aacatacggg acaaatcgac tggctgtcta tccatccctt caatggtcaa
     6361 cggatgtttc aaattgaaaa tacaaaagat cggtttagaa atcaatgagc tggatgtatg
     6421 gaataacccc atatttgtat gaggaatacc gataggtgct tgttcatatc gcttaagcag
     6481 ccggcgatga accacctctt catccgttac tagtccttca ggaaaataag gaaataagct
     6541 gcgaatcgtt tcggaaatcg tctcaggatt atccagttct tggataaaaa aacgaccgag
     6601 taattgattg atttgatcca tgaaatgcat tgtttcctca taagtatctt ctatcatcca
     6661 ctcgttattg ctgttatacg taattttttg cctttttttc ttctcaatat gcgaaaaagc
     6721 ttcttttagc tgcttgactt catcttctag cagtagcggg gagactaaaa tatattttcc
     6781 accgtatcct ggtaaaatag atgtagaaat aacaacatca tattcttctt gtaaatcgat
     6841 tttttttaaa tcagcaatcc gatagaaatg aatttgttgg atgaagggaa aatacctttt
     6901 caatcgtagt tccaacatgc tagtagaagc taatccactt ggcgaaaatc ctgcaatact
     6961 gatattgctt tctttcggac ttcgttccaa tgaattcgca aaatgaagga ccatataagc
     7021 gatctcttct tcggataatt ttttttcttg aaaaatccgc tctactgctt tacggattgc
     7081 attcgcaact tcctcgtatt gaaccatgat tttttctaaa atcggattgg tcagtgtctc
     7141 ttcttgtaaa acggctcttg aaaacacacc agataaatga gtcaacagca ttttgtacaa
     7201 agtttcatct tcataaaaat ggacattcgt ctcttcactt acttcttgaa tcagttgttt
     7261 tactaaataa gcaagctgtg tatcgaacgt ctcttcaaag aaattctgat caaatgagtt
     7321 cgtgaagtca tttaataatc ctgcaaaaaa gacgatctca tttactggat aaagttgttt
     7381 tgtctcagaa gcgatccgtg aaaacaatcg tttagctgtc tgtagtaatt ctttgtttgc
     7441 cagactatta tatgtctcat ttgtcagtaa atgattttta gtcacacgct ccatcgtcaa
     7501 tgtcagcatc aaaaccaaat gttcaagctt tctatcactt agattggtct ttttttggat
     7561 caattcttcc ccgattactt tgcgtgcaag agatagatgt tgcggatcaa cgaatcgaaa
     7621 aaaataattc agtgatgcat cgaaagaaat actatgaaaa agttcatatt cattgatttc
     7681 taattctaat acattcgctg tcagaagccg gcggtatttt tccggtcctt cgatctcgta
     7741 tccctggttc cgaatgattt ccaaaggcag ttggcgaata ctttcttcca actgtttgat
     7801 atctgcataa aaagtggtat tactgatgcg atatgattcc atgaaatgag caagactcaa
     7861 cggctctgtc gtcagtaaca actcggctaa aatcgcgtgt tgtcgtttag cagtggataa
     7921 gtcattactt ttcccatctg tattttcttt taattgctct aatacttctt ctgatgcctc
     7981 aatgacaaat gagcctctag ctgctttttt caaagaagca tttaccgatt tcaatgagac
     8041 ttcaagatta tctaattcgc ggtaaatagt ccgtttactg accttcaata aattcatcat
     8101 tttttggata gatacaggtg acgggctact gagaaattct gctaataatt gtttttctct
     8161 gggcgataaa tacatcttat atttctccct tcacgagttt ttcgtattca ttcgtagcta
     8221 cccaatgttc aactagaact agccgaggga tcttttcttc tagctgttca gttacagcta
     8281 gttctctttg tgcgatataa atcgtttctt cttggatttt gattttttct attgattctt
     8341 ttgagataac aaactgtttt ttcgcttttt ttgctaattg acttaaaatc gtcatgccca
     8401 tagtttgtga gccgcgtctg ttgtttttat acaaaaatac aatattagca ggcgactttt
     8461 tttgttctgt gatatctgct atctcttttt tttcttcccg ctttggaaat tggatcgctt
     8521 cttttattcg tgacagcaag atatctgttt ccaaaaagtg atccagtaca tagatttgac
     8581 tttgaggtac tttttctctt gcaagacccg ctagttcatg ttgcacgata accagctgat
     8641 ttacttggtt attcaattca tagatagagc tgtatccgac agaaagatcg attttttctg
     8701 ctgctaattt ctttttcagc agggcggctc ccatagcact tgatcccata cctgagtcgc
     8761 aagcaaaaat gacggaatga atgatagatg gttccaagat ttctcccctt tctttgaccg
     8821 ttttgttctc tttttcttta tttggtaatt tcaatagtat cgctgcacaa atgaaggtca
     8881 caaatgtact gattgctatc cctatactta ccccagctag ttgtggataa ggcgcattgg
     8941 caaaaatcac gagtagtgac ccaggcgata caggtgcagc taatcccgta tctaaccatt
     9001 gaaatatcaa cgtgccgctt gccccaccag ccattacagc taaaaataga tacggtcgca
     9061 gcaagacaaa tggaaaatag atttcgtgaa tcccgccaat cgcatggatc atcaatgctc
     9121 cactcgcatt tactttttcc tgtttttctg caaaacaaag gatagaaagt aagatcccta
     9181 aaccaggtcc aggatttgcc tccatcaaaa ataaggaaga gtacccgact gtttgtgaga
     9241 attcgattcc taaaggtgtc aatacaccat gattgattgc attattgaaa aatagtactt
     9301 ttagaggttc taagatcaga ctgacaagtg gtaacagatt cattgtgact agccaagaca
     9361 caacggaggc gagcaacaag tgaacactat ttaaaactgg ttcaataaaa acgattccaa
     9421 ttatacaaat aacgctacca gtcagtccta ctagtaggtt gctgacaagc atttcatatc
     9481 cagccttgaa tttcacgtgg aatctagaaa caaattgttc atatacgatg ccggcaagcg
     9541 gcccgataat catcgctcct acgatttgtg gagcattgga acgagcgatc aaccctaaag
     9601 aagcaattgc accggcgact gccccttttt gtccccctac cgatttcccg cctgaataac
     9661 tgatcaagat gggtaaaagc agctggatca tccaatggca tacctcttct aatgtggtgt
     9721 gcaaatcgcc agacacacct tttgttgcta atgacaagat tccccaagcg ataaacagac
     9781 ttagatttgg catgatcatt tcatttaagt gatgtccagc ttttctcagt tgtttcatag
     9841 acacgcgact gctgttgttt gctttccatt tgtattccat ctttttcacc atacttctat
     9901 ttattgatgg acggatttta tgtagaaaaa gcagcaaacg aaaaaccgtc tgctgctttc
     9961 agctatctgg gaactatttg cctggttcag tcgcaatgat tgctagttca ccttggaccg
    10021 cccactcaga aacgccattt ggaataatac agcttgttcc tttttctaat ggatatgttt
    10081 ttccgtcaac gacaaggtct cctgctcctt cgattaccgt catcaatgta taaggggctt
    10141 gttttttgaa agatgtgatt cctttaatat cccactcata tacattgaaa aactcagttt
    10201 ctaaataagt gacgatcgat gaattccctt ttcggacttc tttgatttgt aattctggtg
    10261 ttttcgcagg gacagttgtt acatcgatcg attgttggat atgcagctct cttgtttgtc
    10321 cttgcgcatc ttttctgtcg tagtcataca cgcggtaagt cgtgtcagaa ctttgctgtg
    10381 tttctaaaat catgattcct ttaccaatag catggatcgt tccgcttggt acataaaaga
    10441 aatcaccttt tttaactggt actttcttca aaaggtcatc ccagcgccca tctttgatca
    10501 tctcagctag ttcttctctt gtctttgcat gatgaccata gatgatttct gcaccaggtt
    10561 cagcatcgat gatataccag cattcagttt ttcctaattc accttcatgt ttcatcccat
    10621 aggcatcatc tggatgtact tgtacagata gatcatcttc tgcgtctaag atcttgatca
    10681 ataatggaaa cactggttct gaaggatggc caaacaattc cttgtgttct gaccatagtt
    10741 catctaatct tttccctttg aattcgccat tttctactac actgacacca tgaggatgcg
    10801 cactgatcgc ccaatcttct ccgattttat cgttagggat atcaaaacca aagactgaac
    10861 gtaaacgact tcctccccaa attttttctt gaaaaactgg ttttaaaaac ataggttcct
    10921 gcataattca aactcctctc atttttcata gctattatag cattatttcg gtaataccgc
    10981 tttctctttc aaaaagtttt caacaatttc atctcgacat atgagatact ccgcacgatg
    11041 atcgacagga tgtacgcgat ttgacaaaaa tatcaacgct tcttgtgttt ttatatcaat
    11101 caagaaaaac gtacctgtat aaccagtatg aaaaagaatc gcatgaccgt cttctacatc
    11161 cttttttaag tcccatccta aagaacgata gccatttttt ttcggtgttt gatcttgcag
    11221 taaagaatca atcgtctctt cttctaaaaa acgaccatta cggatttttc ccttgttcaa
    11281 atacatttgg acaaaagtaa tgctgtcttt caaatcaata aagagacccg cgttacctgc
    11341 atgttcagcc aaaactcttg ctttcggatc atgagtttgt ccacgcaaaa cctcacccga
    11401 aggcagttgt tctgtgggaa cgattttttt ggaaggaaaa ggaagaaata agctgttcat
    11461 cattcctaat ggttccaata cttgttcttg aaagatttcc actgcagttt cttggtaaat
    11521 ttcctctagc atcaacccaa gaagaatcgt accggcatct gtatatttta cttcttttcc
    11581 tagttgctca cctggttcta gcttcaaata agcggcgatc aattcttcct gagatagctt
    11641 atctctgttt ggtatccatg ttttgatatc agaagtatgt gtcagtaaat gacgcaaagt
    11701 gattgtctga ttttcaaatt caggaagata tttatgaagc ggttgatcga gttctatctt
    11761 tccatcttct aatagacgta aaactaatga agtggttgca acgactttcg tcagtgaggc
    11821 tgcatcaaac aacagatctt ctgtcatcag ctgcttttca ggaacgactt gggcaaagcc
    11881 ggctgtaaat gtttctactt gattttcttt tatgaaggcg actgctacac ctggataaaa
    11941 tccttcttcc atctttttca aaataagctg tttcgtgtgt ggatacatct ttttctcctg
    12001 cttttctatc agattcttac agtccaagct taaagcttaa tctatgagga atcaaccttt
    12061 tttacacaaa aaaactgcgc gaaaaaccgc gcagtttccc ccggtatact attttctctt
    12121 tctatctcta gaataagcga tagtatgacc gttcataaaa tgacgagaca tgcgacaatc
    12181 gttaagaaag aaaaagttaa gcgtcgcatc gaatgtcagt tttattcttt tcttttggct
    12241 cactgacgaa ccaccattcg attcctatag catcatagat tgtgagtact gtgtgctttt
    12301 tgtctaagaa gaaatcaact gatagttgct gcaagtgagc aatcagtcct tccatgcttt
    12361 cagtatcgat catgaatttc agaaaatcaa gtccaatcac ttcatctgta ggcaaatcaa
    12421 ttgcgcctcc gctagcttct gtcaaaccaa catggaaatc tccttcattc aatacatgga
    12481 tgccctcttc ttcattatgt accttcaaac caagtacatg attaagaaat tggcgttctt
    12541 ttgttaagtt agtaacattt aaatggattt tttcaaaata agaacctgtt gatagtttac
    12601 tgaatttttc ttcagatttt cttaataatg cttcttgatc cattgtttct ggaatttctc
    12661 tttggtcgtt cttattctct tcatggtaaa tttccaattc attctcttct gggtctacga
    12721 gcaaaatccc catacgtcca tgatcttcta aagcttcttt gattggataa ttcattttac
    12781 ggatacgaca aaggatatct gccatttctt ctgtgctagg gataatcaat gaaaaacggt
    12841 tcagcttttt gatcaccccc atgtgatcat tcgctctagg actttcttcc agcagcaaca
    12901 tttcacttcc agcttcttct gttcctaaaa tggctaattc gttttcttct cttttcaaat
    12961 caaagccaac aacgttcttg taaaaatcaa tcattttatc tcggtctttc acccgaatgg
    13021 ctacactttt taaataggtg gcagccccca attgaaaatc tttcatattt cttgctcccc
    13081 tctacgtcaa gctttatttt aacgatggat tcttcttctt tcaagcaata actttgattt
    13141 tgttaaaaaa aatgtgagaa aattcgcatt ttgcttgttt tttgtaaatt aatgagtaaa
    13201 aactcttata aaaagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
    13261 acaaaaatag ctcctcctat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt
    13321 tgaacaccgt ttaaggaaga ggttgtaaaa tcagcggttt gacctgagta ttagagatac
    13381 aaaacggaaa aatagcttct catattttgc cgttttttga gctaataccg aaggacagct
    13441 gattgaacac cgtttattcg gaaaaaggga ctgtgacacg acttttgtca cagcccctaa
    13501 aaaaaataat aaatttgtac tattctatga agcttcttct tcagaaaact gactgttata
    13561 aagatctgca tagaatccgt ctttttccat caatgtatcg tggtttcctg tttctacgat
    13621 cgaaccatga ttcatcacaa taatgttgtc tgcatcgcgt attgtagaca agcggtgagc
    13681 aacaacgaaa cttgtccggt tttccaatag gcggttcatc gctttttgaa tcagtatttc
    13741 tgttcgtgta tctacactag atgttgcttc atcaaggatc aggacatctg gatttgctaa
    13801 gaatgcccgc gcaattgtga ttagctgacg ctgcccttgc gaaatattgc ttgcatcctc
    13861 gtttaataca gtgttatatc cttctggcaa tttacgaaca aaatcgtcta cgtgagcagc
    13921 tttagcagcc tcaatgattt gttcgtctgt tgcatcgtcg ttcccatatt tgatattgtc
    13981 atagatactt cctgtaaaca gccaagtatc ttgcagtacc attgaaaact gtgcgcgcaa
    14041 ttcatcacgt gaaagatcac gggtatctac accatcgtaa cgaatactac ctccagaaac
    14101 atcgtagaac cgttcaagaa ggttgatcag tgtcgtcttc cctgcaccag tcggacctac
    14161 aatagcgacc atctcaccag gtttgacatc caagttaaaa tcagtcatta gcaagttatc
    14221 tggtgtgtag ccgaattgaa cattttcaaa acgaactttg tatggtgtat ctttttcttc
    14281 tggaaggcca gatttttcat tggacatctc ttcttcatcc aacacttcaa atacccgttc
    14341 ggctgaagca actgttgatt ggatagtgtt catcaaactt gcaagttgag tgattggttg
    14401 agagaactgt gttgtatatt gcaagaacgc ttgaacatcc cctaatgtca tgttcccatt
    14461 tgcaactttt actccgccta gtacagcgac gaacacatag cctagatttt taacgaaatt
    14521 catcaacggc atgatgattg ccgaaataaa ttgagctttc caaccagcgg catacagatt
    14581 ctgattttct ttttcaaata cttcctggtc tttttcagta tgattgaatg ttttaacaat
    14641 ggtatgacca ccatatgttt cttctacttg gttattcaat aaacctaaac ttttttgctg
    14701 atctgcgaaa aacacttgtg atttcggcgc tacgatcatc acgatgatca agcttaaagg
    14761 gatcatcaat aacgcgacta acgtcaattg ccagctgatt gaaagcatca tcacgatgac
    14821 cccaatcaat gtgactgcac tagttacaaa ttgagtcaaa ttttgctgca gcgtgcttgc
    14881 gatgttatcc atatcattga ttgctcgaga catgatatcc ccatttgtat gtgtatcata
    14941 ataggaaatc ggtacacgat tcattttggc ttccaaatca tgacgtaatt cataaactgt
    15001 tcgttgggaa acacgtgtca taataaattg ctgtaaaaag ttgaatacag cagaaatcaa
    15061 atacatcacg attactgtcg caatgatctg cgcgatttta tcaaagtcta tcggaaattt
    15121 tgttacttgt tggccttgtt gcatctgttg agcgccagcc attacacctt tgaatatttc
    15181 tgtggttgct ttccccaaga cctttggtgt ttggatttgg aagatcacgg ctgctattgc
    15241 tagtacaaat acagcgatga tcgcaaccat acgtttagac atatagccaa acaatcggcg
    15301 taccgttccc cagaaatttt taggcttttc accttttaca ccgagatttc tgccaggtcc
    15361 accatgtcct ggaccgcctg ctggtccatg agagggacgt gtgtttgttt tactcatgct
    15421 aagtcctcct ctcttagttg tgaatgaacg atttcttgat aagtttcgtt tgttgctaat
    15481 agttcttcat gtgttccttt gccaaccagt tttccttcat ctagcacaag gatcatatct
    15541 gcatccatta ctgtacttac tcgttgagca actaaaacgg tgatactttc tttcatattc
    15601 gttttcaatg cttggcgaag attggcatcc gttttgaaat ccaacgctga gaaggaatca
    15661 tcaaacacat agatatctgc tgttttgact aatgcccgtg caattgccag acgctgtctt
    15721 tgtccaccag agaagtttcc tcctccttgt tccacatggc tatctaagcc atcttccatc
    15781 tctgaaacaa aatctttagc ttgtgcgatt tctaatgctt tccaaatcat ttcatctgtt
    15841 gcatccggct taccatactg catattgtct cggatcgttc cagagaacag aactgctttt
    15901 tgaggaacaa agcccattaa ttctcgcaaa ttatattgag tcatgtcact aatgtccgtt
    15961 ccatttattt ggatcgaacc agattcgatg tcatataatc gagggatcaa attgactaag
    16021 gtcgttttac ctgaaccagt cccccctatg attgcgacga tctcgcccga cttggcttgg
    16081 aaatcaatat cttccaatgc aagtttttct gctccatgat atcgataatt gacatgattg
    16141 aaggccaatg ttgcgttttt tccagcaaaa gaaactgttt taggattttc agggtcatgg
    16201 ataccgattt tttcatccaa cacttcatta atacggtcag ctgaagcttg tgcacgaggc
    16261 accatgataa aaatagcgga aagcatcata aagctgatca agatctgcat cgcataagtc
    16321 ataaatgcaa tcagattacc gacttccaaa gtcatatctg ctatgtagtg tccaccaaac
    16381 caagtgattg ctacgtttgt accgctcatg atcaatgtca tcattggcat catcaatgcg
    16441 acgatcgtat taactttgat tgccgtattt gtataatctt tattcgctaa atcaaaacga
    16501 ttttcttcaa agcgtgtctt atcgaatgct cgaatcactc tgacccctgt caatccttct
    16561 cgaaatacca ggtttaaacg gtcagttttc ttttgcatac ttttgaataa tggaacagca
    16621 aagtacatga ttccgccgat caaaataatc attactggga tgacaactaa gaagattttt
    16681 gtcaactgat gatctttttg ataagcaagt atgctcgcgc caatcaacgt gattggtgca
    16741 ttgatcatca tacgtaaaaa catttgtgta accatttgga tttgagtcac atcattggtc
    16801 gttcttgtga tcaaagaggc cgttccatat ttatcaaacg catcttttgt cagactctct
    16861 gattttttat agacatccgt acgcaattgc ttacccagtt tctgtgattc acgagtagca
    16921 aaaaatgtat ttgcgatagc agctaagata ctgatcaaag aaaagccgat catcacaaaa
    16981 cctgtatgcc aaatataatc gacatctcct tgcgctaccc ctttatcaat aatattggag
    17041 gtaagtgtcg gcaaatataa atctgccagt acttggatca ccatgaaaac tgcagctgca
    17101 attgctgaag tcaaggagat ccgttttacg atttttagca tctagttccc ttctttctat
    17161 ttatatactc cgtttttcag ccaagaaagt ttcaaattga attcactgat tgcttgctct
    17221 actgagtaat cttcagatgt tgaaaataag cgatacgaat gcgcaattgt tgtaaataca
    17281 gtattcatca ctaattggat cagtaattcc aattcatgat cattttttac tttcagcttc
    17341 gtccgatcga tcaattcaag caactctttt gcatcttgtt tgtgttcttt atgtggatcg
    17401 tgaggcgttt tttggcgact cgtatgagga gcgacttttc ttgaggcacg gtaatccatg
    17461 ttcataaaca agttacgata aaaagaagca ttctcgcctt ccaaaatctc ataaatcatt
    17521 ttagaaaaat aggattcgaa tcctttgaat agatcaccat cggattcttt cattagttgt
    17581 aacatatctt tagagctgtc acgtctcatt gtctcaaagt aataaaaata aagatcctct
    17641 ttatcttcaa aatactgata gaaactgcct ctagggatat ccgctaattt gacgatatta
    17701 gcaatcgaag cttcttttaa gggtactcta gaaaactcga ttctcgcagc ctcgattagt
    17761 cttttttgtt tttctttagg caaatggaaa aatgtctgtg tcggcatatg gatcctcctt
    17821 tctttcaaga tgacacattg tcatatgacg ctgtgtcatt ataacacctc ctacctgttt
    17881 tgcaagacat ttgcatagtt tttttcatta tcatccgata cattaaaaaa gtgataagag
    17941 gaatccttcc atttgaatta atgagagttg cccattaaaa tagccacata ctaaacaaag
    18001 gatgagataa atggcccaat caattagtgt ccaagacttc aataaattgc ctctcgatgc
    18061 tgacagcatc gttttagata caagaaatac cttagattac acaaaaaacc acttaagcca
    18121 ctcgctttct atccccgctg ctatcttgcc aagacaatta aaaaatctag atccaaagaa
    18181 actttattat attctaagct atacaggcag gcgatcagaa gcattagccg atcaacttac
    18241 tgctaatggt tttcgtgctg tttatatcat tggtgggatg gaagctcttc agtcgagtgt
    18301 tgcttgaata ttgcttgtta gattcaaaag aggctgtgac aaaaaatcgt gtcacagcct
    18361 cttttatacc ctgtcttttt ttagttattt attcttcaac cttatcaata tgacttgata
    18421 gccttttctt tttctcatag ttataacaat catagcaata aaaggagaag gtaagcatat
    18481 ggcatctgtt ttagtagtca aaggtcatcc cctaactgca gaagaatcgc gcacagttaa
    18541 ggcattgacg tcttttttaa caagttataa agaaaatcat ccagatgatg aagtgactgt
    18601 tttggaactt tatcgcgatg atattccgga aatcgatgaa gaattattat caggttggga
    18661 agcattacgt gctggtgcag aattcacttc cttatcagaa agtcaacaac aaaaaattgc
    18721 tcgtttcaat gaattgactg atcagttcct tgctgctgat aaaatcgtta tcgcaaacgc
    18781 cttatggaat ctaaatatcc caacaaaatt aaaagcttgg ttcgataccg tcaatgtagc
    18841 tggaaaaacg ttccgctata cagaaaacgg gccagaagga ttagtgacag gtaaaaaagc
    18901 actgcacatc caatctaatg gcggtgtata caacggacaa gactttgcct ctcaatatgt
    18961 aaaaggtatt ttaaactttg taggcatcga tcaagtagat cagctattca tcgaaggaat
    19021 cgattatgac cctgaccgtg cagatgagtt gatgcaaaat gcattagata aagctgctgc
    19081 tttaggtaaa tcattttaaa aattgagata aatgcataca aaagagacag aggaaaaaat
    19141 cttcaaagca gcatttgtac cttggatatt ttcccatctg tctctttttg ttattctttc
    19201 tgctttttca acggattttc ttcataaaga gaagagattg ttgtgtacca ttcaaaaaca
    19261 tgagtaatca ctactttagc tgctgcataa acaggaatcc ccaagatcac accgatgatt
    19321 ccgaacaatt tcccagaagt cagcagaact aataaaatcg ttactggatg gattgctaac
    19381 tgattcccta gtactaatgg agagatcaag cgtccttcta ttgtctgctc tactgcaaaa
    19441 acgatcagta ctttgatgat catgaatgga cctgctacaa tggctaaaaa gatagccgga
    19501 atcatcgcta aaaatgatcc taagtaaggg atcaggttca aaaaacccgc aataatcccc
    19561 aacgtgacag catactctaa accaatgacc gcaaaaccaa tcataaacat gattgccaca
    19621 gcaaaagcca cagttagttg tcctcttata taagaagaaa cttgactatt catttcactc
    19681 agcacattca acgtcggttt ttgcatgcgt gtcggtaaaa atttgacaaa ataaggtgct
    19741 aattgtttac cgtctttcaa tagataaaat aagataaacg gcatcgtcag cagagccact
    19801 aaaatagtcg ctaccgcgcc gacaaaactt ccaagactct gaactgtact cttagatata
    19861 tcttggatca tatcgcctgc cgaattcatg aacttatcac ctgctgtttc caactgttcg
    19921 cgaaattggt tgaacagcgg gtcccgcaaa atctcggtta atttattatc aatcgtatct
    19981 acatattgag ggaagttact gatgaagctc actgtttgtt cctgaatctt agggatgatc
    20041 actacgctcc cccatgcgat tagtgcaaca accaaaacaa acagcccaat gatactgtaa
    20101 agccttggta cttttcgctt ttcaaagtaa tcaactaccg gattcatcaa gtaatacaag
    20161 attcctgcta aaatgatagg cagacctacg attgctaaaa attgccagat tggtgcaaac
    20221 aaatatgaaa cttttgtaaa taaaaagacg atcagcaata ttaacaatac aacaagtaag
    20281 gctgtgacta cttgattatt taaaaaccag cgccaaaacc acgataccgg tctgttcttc
    20341 ttattttttc cttccataat tcctcctgtt agttataaaa aatttggctt cccaaaccaa
    20401 tagcaggcag tctatgcgga tgaagataga ttccatttgc aatttgtcct tcttctggac
    20461 aagcatcaaa aatcaatgat acgtgagcta ctgtctctaa attcccattg gccatcggac
    20521 cgacataatc gatcgtatat acctgatcat caaaagcaat tgttccgcct gctttcaaag
    20581 aaaaaggttc gtcttttgat acttcttgaa tcaccgaata ttttcgtaag gcagctgttg
    20641 ctgttttatc gaacaaaatc agcatgggtt cttccttgtt tattgcttct gtcccaattt
    20701 ctgtaatctt tgcctgaatc attgcactct ctcctttaat tgctttcaaa aacagtataa
    20761 cacattctca ttctttagcc gtgtggaata attatgtgat agaataaaaa cgaaaactat
    20821 ttcattacaa ttaggaggaa tcagctcata tgatcgaaaa gataatttta ggaacctata
    20881 cgcgtcgcga aagtaaagga atctatacga tcgaccttga tacagaaaaa gaagaattag
    20941 ccaacctttc attagctgtt gaagaaaata gccctaccta tgtagcaaaa agtaaagctg
    21001 gcaatcttta cacagttact agtgtggatg gattaggtgg tgccggtgcg tacgataagg
    21061 attttcattt tttgaatgcg gtaacagagt ctggcgctcc tttgtgttat gtagcggttg
    21121 atgaagcacg tcaattacta tacggggcga actatcataa aggtgaagta aatgtctacc
    21181 atatattagg agatggcgaa ctgaaagcag cagattcaat attccatcaa gaagcgacag
    21241 gtcctcataa aaaccaagat catgcgcatg tccactatac ggatctgact cctgaccaac
    21301 gattagtcgt atgtgatctt ggtacagatc gtgtgtatac ttatgatgtg tccgaaaacg
    21361 gcaaattgaa tcttgtaact accttcactg cagagccagg aacaggccct cgccatcttg
    21421 tctttcatcc aaatggacaa ttcgcttatt tatttggtga attagacagc acagtccgcg
    21481 tgctttctta taataaagaa acaggtgctt ttgaagaatt acagaaaata tcgaccttgc
    21541 cagaagaatt cgacggtgaa aatggagggg cagctatccg catttcaaat gatggcaagt
    21601 tcctttatgc ttctaaccga ggacataact cgattgctgt attcgcagtt tttgaagatg
    21661 gttcccacat tcaaaacaag caaatcatct caacagaagg ggattttccc cgcgatttcg
    21721 cactagatcc aagcaataat tttgtcgtgt gtgccaatca gaacaccgat aatctgacat
    21781 tatatcgccg tcatcctgaa acaggattac tggaattact gaaaaaagat gtctatgtac
    21841 cagaatgtgt ctgtgtttat tttgatcgtt agtaagaaaa ggagaatgaa atgaatcagc
    21901 aagacgtatt agacagacta cgggaagaac taaatattcc tttctttgat gggaaaatcg
    21961 aagataaaga gtatagcgaa gaggaatatc agaaactcaa agccgattta cagaactact
    22021 ttgaacaata tgtacagaat gtagaaaact aatcagcttt ccatactatc tgaaaagaac
    22081 atcaggacaa gacttttccg tctatcctga tgttcttttc tacaaataaa aaaccgccga
    22141 gatggttaga ctcgacggaa aaggagtatt tactcgattt tgagtaagta caaggttgtt
    22201 gttggttata ggtatgtcat tatcataaag gattttgatc actctatcat aaaggtaata
    22261 ttacatttat attagataac tctagttttt attgttatca aagatttctt ttattttttc
    22321 aacaacaaat tagtcatttt ttggatggca gctgtcaatt ggtctgtcgc ttggtcttgt
    22381 tttgcttcag atgtattgaa tagatcttca tctacccctg attcaagatc gatactttgg
    22441 atcatttctt cacagcctgc tacatatgaa agataagctt tttcaaaatt tttatgaatg
    22501 cccattgctt ttgctggtgg acgcaaagta ctgactttct taagcattgc ccgatatttt
    22561 tttgttcctt cttcaaataa caggatgatc tcttcaagag tcgatttgtc cagttcactt
    22621 gtttttcctt catcaattgc gtttctgatc acttcatatt tcggatgcat tccttctccg
    22681 attgcttctg tttccttgac gatttgatcg atggactgca cataatagcc aatattcggt
    22741 aacatgttcg ttttcctact ttctccattg ttgttattat ttattctact tgtttttatc
    22801 ctcaaatgac aagtacttga aaaaatttaa tggtttctat acagctcgcc attcttattc
    22861 gtgctcaaag aaaccatctg ctaaactata ttgtttccct gcgacaatgt catattggat
    22921 caattgatat tcgtcgtaaa tctcttgttg cgagcgattg aattgcgctt ctttatacca
    22981 ctgtccgttt tgatagtaca gttctcgttc aaacgccgga agttcctgtt ctaatgataa
    23041 aagcagctga taaaagccat ttgttgtctg attcgtctgt tccattagat tcgctgcaaa
    23101 ataaaacgga ctggtgattg catcgtgagt cgtttgcttt tctaattctc ctttactgtc
    23161 gaacataaga aattgtgttt catgcaatgt ttgtttgtca ttttttgctt ggatcgtatc
    23221 tgaatagatc cctggaagat gatcccccca aaaaacaact aaagtccttc tagaaagatt
    23281 cttcaattct tccgtgaact gcttcagtgc cgtgctgcta taggaaatat cttgcagata
    23341 attctctaag tcaagtgtcc cacttccatc tgaagttttt gcaggatagt ctagattatc
    23401 atactttcca tcgtagggca tatgtgtctg catcgtgact aaatgaatga actgaggtgt
    23461 tttttcttcc ttgagaagtg tcattacttc tttgtaagct gactcatcag aaatatatgg
    23521 attattctct atcgtatccg tataggtcat cgtacgctcg ctgatgaact ggtcaaatcc
    23581 taatgtctga taaacatcct ctcttttata catcgaagta ttgtatggat ggatcgctgt
    23641 cgtctggtat gattgtgcgt ttaacgctga aacgatagaa ggaagctgat ccatttttgg
    23701 tacaagcatc gtgtaagggg tggtgagttg cgcattaaat aacgccatag aaaaaccagt
    23761 caatgcttca aattcgatat ttgctgttcc accgccatag ttttgagaaa gcatattgcc
    23821 actatatgtt tgatcagcta cttcataata atctgccaat ggctcaccag atacttctac
    23881 gccgtttaat cgactaggat ctgagaaact ttcactcatc acgaacacga tgttcggcga
    23941 ctcttcctct acagttttat ttttttcatc ggctagcttc tggtattttt cagtgatttc
    24001 tttgatcttt tctttcgaat atccttcagg ttcatccatt ggttctacct tcagattgta
    24061 caaaaaccca ccgataaaac cagtgttata gtagttcatt ttctggctgt aaggtatcca
    24121 tagtgccgtt ttattatatg cttttcgcaa caaattatcg ggattgttga aattactgat
    24181 ataccctagc agtccaattg ccacaagcag tgtcgttaat cggatcaatt ggaatcgcct
    24241 tccttttttc aggcttcgaa acatgtacca gcaaaacaat cctatcacgg ttcctgcggc
    24301 agctaagatc actgtaaaca gcatagtgcc ggtcatatct ttcagcaatc cgatttcagt
    24361 gatcatcttc aaatcgtctg gataaatcgg ttcttgccga taactcattt tcatataatt
    24421 tgcaaaacct aatacaccga tggctacgac atacgtaagg gaacctaaag ggacagatcc
    24481 tgctagtgca atcagaaaaa taagaataat cagcaagacg agacaggcta agaagaattt
    24541 ttccgtatgc caagaaaaag caaatttcaa cgcaaggtct acggatagat cattttgaca
    24601 ccactgcaaa aataaattac taaaaacaac aagaaaaaag atactgatga ttgctgctgt
    24661 tccttttaaa atctttttct gtttggtcga aagtctgctt atatatcttt tcaaatgctt
    24721 gctccttcat ttctatcaat aaaagaatgt tgcttctctt attttagttg tacgaaattc
    24781 gaaagtaaag aacggaaaga agaattaatg aaatccaaat attctctttc tttatggcct
    24841 ttctggtttc tttaataagc ggacctttcg tactccagaa attttttcta gctgttgttt
    24901 cacttgattg agctgttcaa aatcagtact gtctatgtca atcaatatgt agacatgctc
    24961 attcttatgg ttacgcgata tatctgcgat cgctagctcg ttctcattca gcagagaaaa
    25021 tatctctgct aatatattgg ttttcttttg ataaaatata gtgaatcgaa acggcgcttg
    25081 gaaaaacatt cgtgcggatg gataattgac cgcttctcta accgtaccga acaaaagata
    25141 atcttttaag gcttcaactg ccaaacgtcc gctgtctgcc aaagcttcct gagtcgatcc
    25201 ccctagatga ggcaacataa gtatacggtc attttctaag aactcttctt ctggaaaatc
    25261 agtcaagtat ttagccagat gttgtttttc caatgcttgc ataacagcat atttatcaac
    25321 aatttcactt cttcctacat tgataagtac agcatctttt ttcatttttt ctatattcgc
    25381 ctgatcgatc aatccttttg tatcttctgt caacggcagc atgactacga tgtaatccga
    25441 ggtagaaaga actgtttcta aatctgcttg ccgaacataa tccaactggg catcgcggat
    25501 agaataaccc aagacatcca tccctaagct atagcaactt agcgctactt ttgtgcctat
    25561 cgctccaaga ccaagaagac cgatggtttt gccttgcaat tctcgtccca cataagcact
    25621 tcttttgttt tctgcctgtt ctaaaatatt aggacctgtt agtgtttgta ccatacgact
    25681 tgcttcgatt atcggacggg aagataagag caaacagcac aaaacaagct cttttactgc
    25741 attagcattt acacctggtg tattcatgac gatcgtcccg ttttcagaag ctttttcaac
    25801 gttgatcgtg ttcaccccaa caccagctct tgaaattgct agcaaatctg gtgtaagcca
    25861 ttcatctttc actttcgtac tacgaataac tattccttcg gctggcaaat catctattat
    25921 tttgaaatat tccccttgta acttttctat ctcttgttct ggaatagcat caatcaaatg
    25981 aacgtgatac ataaaacccc ctccttacta acaagtatct cgaaatttct gcaattagac
    26041 aactgtttac catttgactt cctagaagca aaaagagcag ttagagcaga gtaggctgag
    26101 tgataaaaat aaggctatga caagttactg ccataagcct tattttcgag agcaaaatat
    26161 tgagggtgag atatcttgat aaatggtgtt ccgttagctg acattgataa taagactaaa
    26221 tttcaaaaaa gttgaaaagc aaatttcgtc aatctcttct tatttcttaa aactaagtgg
    26281 ctttttcaca acctcttttt atctaataac cactgtttcc aactcaacta attttgccca
    26341 cgtttcaatt tgatctgttg ttagatttag cgagacaacg gtatgatgac cgccacctgc
    26401 ctgaatccat gaatggacac cttcatggaa attcggttta gttttccata acacacgtgc
    26461 aacaggtaaa ttaggtgctt cagtcgttgg ttcaaaggct tctacttcat tgatcagcag
    26521 tttataatgc gtaccgaaat ctgccatcga tacgactacc ccttctcctg ctttcccatc
    26581 aaatacaaga cgcgccggat cttctctatc tcccattgat aaaggagaaa caacgattct
    26641 tggtttcgtc gcagcaagcg tcggatctac ttccatcata tgggattcca agattgcttc
    26701 ttggccacta gctagctcgt aagtataatc ttccatgaat cctgtatctt tccctcgagc
    26761 catgattttc agcaaccgat caatcgctgc agttttccag tcgccttccc cagcaaaacc
    26821 gtatccttct gccatcaaac gttgtacagc tagtccaggt agttgtttca ttccatacag
    26881 gtcttcaaaa ttagtcgtaa aagcgttata gcctccttct tctaagaaac ggcgaatacc
    26941 gatttcctgc tgagcttgta cttttacatg ttcctcccat gtcttttctt cataatcacc
    27001 atagtcgaag gtatacaatt ccttgtactc ttcaaataat tgatcgactt cttcgtctga
    27061 tacgcgatcg attacttgta caagatctcc gataccaaaa taatcaacag tccaaccaaa
    27121 ttgaatctgt gcttccactt tgtctccttc tgtgacaccc acgtttctca tgttgtcacc
    27181 aaaacgagcc acttttattc caaaactttc attataggct actgcaacat ccatccaatc
    27241 agcaatctct ttttggatct caggtcgttt ccagtagcca acgacgattt tattttgttt
    27301 atttaatcga gcattgatga acccatattc gcggtcacca tgagcggatt gatttaaatt
    27361 catgaaatcc atatcgatcg ttttccaagg aatgctttcg ttatattgcg tagctaaatg
    27421 aagcaatggt ttttgtagaa gctttgtccc gcggatccac atttttgctg gtgagaaagt
    27481 atgcatccaa gtgatcacac ctgctacttc ttctcgataa ttgacctctt tcatgatttt
    27541 tgtgatcgta tcagcagtaa ctgctaattc ttgaaggcgg attggataag gcaattggcc
    27601 attttcattc agttcatcga ccatttcttg tgcatgtttc tttacttctc gcagtgcttc
    27661 ttccccatat aaatgctgtg atcctacaac aaaccaaaat tctttttctc caatattcaa
    27721 cataattagc cactcctttt atttgttttg tccgtaatac gcattttttc cgtgttttcg
    27781 taaataatgt ttatctaaaa tccgttgagg cagctcttct gaaaatgaat tgagctgtct
    27841 ggtaaataaa ttcatcttgc atacttcatc tagtacgact gcgttcatta ccgcactttg
    27901 cgcatctttc ccccaagtaa atggaccgtg tccgtgtaaa agtacccctg gaattgctaa
    27961 gggatcaatt ttgcgttctt tgaatgtttc tatgataact tttcctgttt catattcgta
    28021 cccgctgtcg atttcctgtt gtgtcaaaaa tcgagcacaa ggtaccgatc cataaaaagt
    28081 atctgcatgc gtcgtcccca tcgcaggaac atccaatcct gcttgtgccc agatcgtcgc
    28141 ccatgtcgag tgagtatgga cgattcctcc gatttgagga aatgaacgat ataaaacagc
    28201 atgagtaggc gtatccgaag agggattgag tttaccttca atgatatgat tgtccaagtc
    28261 cacaacgacc atgtcgtctg ctgtcatctc ttcataactg actccgctag gtttgataac
    28321 gaaatatcgt gtttcaggat caaaagcgct cacatttccc catgtatatt tgacgagtcc
    28381 gtgtttaggc aagtcaagat ttgcttggta tacttcttct ttcagttgtt ccagcatttt
    28441 ctatcactcc tttttcttgc aattgatgaa tcgctgtttt ttcaatgtcg atcccctcac
    28501 ggtaacgtct cataaatatc tgatagcctg ctatttcttc tgctgaaggt gtgatggtga
    28561 ttccttggtc ttgtccaaac acgtcttctg ccaagaagtt ttctaaagac ttcccatgtc
    28621 gatccatcat ataggctgct aataacgcga ttccccacgc tccaccttct ccggctgtgt
    28681 ccatcactgt aactggtgcc tccatagcag atgctagaat ccgctgtcca acttctggtg
    28741 ttttaaaaat ccccccatgc cccactaaac gatcaatttg gacattctca gatttcaata
    28801 tatccattcc gattctcatt gcaccaaaag cactggctaa atggatccgc atgaaattcg
    28861 ctaaatcaaa acggctacct ggtgtacgta caaataatgg ccttccttca tttactcctg
    28921 taatgttttc accggaaaaa taaccataag aaagtaatcc cccgcaatcc gtatcgcctt
    28981 ctagcgcttt gttgaataat accgtgaata atttatctct gcttatctcc atacccaaag
    29041 aattggtaaa ttcttcaaaa atactgaccc aagcattgat atccgaagag cagttattcg
    29101 tatgaaccat gcctactagt tctcctgaag gtgtagtgac catgtcgatt tccggatgga
    29161 cattttctag ttctttttct aatacgatca tagcgaaggc ggaagtacca gccgaaatgt
    29221 tccctgtccg tttggctaca ctgttcgtag cgaccatacc ggttcctgca tccccttctg
    29281 gcggacaaac cgggattcct ggtctcaaat ttcccgtagg gtcgatcaga taagcccctg
    29341 attcgcttaa cttgccagct tcttctcctg ctactagaac tttaggcaaa atgtgatcca
    29401 agtcaatcgc tacgtgcgct ttttcgatta actgaccaaa aatatttttc atttcttgat
    29461 ggtaggtttt cgttgaagga tcgataggaa acatgcctga tgcatctcct acgcctagta
    29521 ctttctcacc agtaagctgc caatggacat aacctgcaag cgttgtcaaa aaattaattt
    29581 ctttcacatg ttcttcttta ttcaaaatag attgatacaa atgagcgata ctccatctct
    29641 gcggaatatt gtattgaaat ttttccgtta atattcgttc tgcttcttca gtgattgtat
    29701 ttcgccaagt acgaaatggt actagtaact gttccttgct atcaaatgct agataaccgt
    29761 gcatcattgc actgaaccca atcgacccga ttgtcgtaag attctcgcca tagatttcga
    29821 atacttctgc tgccattttt cggtaactga cgcgcagtcc tttccaaata tcttctaatg
    29881 aataggtcca gataccattt tctagtttgt tttcccactc ataactacca gcggcaatag
    29941 gagtaaaaga tgaatcaatc aatacagctt tgatcctagt tgatccaaac tcaatcccta
    30001 aagatgtacg accatctatg acatctaaca tactttgcgc atttacttcc ttcataatga
    30061 tttaactccc tccttctcgt caaacctgat caaaccgtct ttttagtagc gcttacaatg
    30121 attattgtat acttttgtac gtacatgtca agcggttatt tacaagaata caataaaaaa
    30181 cacttgtttt gtacgtacat aagttataaa atgaaattgc ttacaaaatc tgatcaaccg
    30241 ccttctcgaa attctagaag gagagaatac ttatgaaaaa agaaaaaaaa gtttcctcat
    30301 cgtttattta tttttttggc gcatttggcg gcattctatt tggatatgat attggtgtta
    30361 tgaccggcgc cttacctttt ttacagcatg attggggttt ggcaggtaaa gcttctttga
    30421 taggatggat cacgtcttcg gtcatgttag gtgcgatcct tggcggctct ttatctggtc
    30481 ttctttctga taagcttgga cgacgaaaaa tgattcttct ttctgcactt atttttatgg
    30541 ctggttctgt cctttctgca tctgcccctc acaatggttc ttattttctg attgcagcaa
    30601 ggattcttct aggtttagct gttggcgctg catctgccct agttcctgct tatatgtctg
    30661 aaatggctcc tgctagacta aggggaagat tgtcagggat caatcaagtg atgattgctt
    30721 ctggcatgct cctttcctat gttgcagatt atttgctaaa agtgttgcct gaaacgatgg
    30781 cttggcgcgt gatgttaggg ctcgctgctg tccccgcgct gattttattt ttcggtgttt
    30841 tagctctacc agagtcaccg cgttttctta tgcaatctgg ccgcttggaa gaggcaaaaa
    30901 gagtattgaa ctatattcgg acaccaaaag aagctgaaca ggaattcgaa caaatccagc
    30961 tgaatgtcaa acaagaaaag acaaccggaa cttcttggca tactttgttt ttagaaaaat
    31021 atcgttcctt agtcattgcc ggtattggtg tagcagtatt ccagcaattt caaggagcta
    31081 atgctatttt ttactatatt ccgctgatcg tagaaaaagc gactgggcat gcagcgagcg
    31141 atgcattgat gtggccgatc atccaaggaa tcattttagt tgctggttct ttgctctttt
    31201 tggttattgc agacaagttc aatcggcgaa ctttattgaa aataggcggt agcgtgatgg
    31261 gactttcatt catcctacca gctgttttag gaactgtact ggatgctcat acaaattcgt
    31321 tgttgattct cttgttttta tgtatttatg tcgcatttta ttcttgcaca tgggctccac
    31381 tgacatgggt aatcataggc gaaatattcc cactagctgt ccgcgggcga gcatctggcc
    31441 tagcctcttc ttttaattgg attggttctt tcctagttgg cttgcttttt cctgtgatga
    31501 ctgcttctat gtctcaagaa atcgttttcg gcatttttgg gatcatctgc tttttagggg
    31561 ttttatttat tcaagagatc gtaccagaaa ctcgtgggaa atctttagag gaaatcgaac
    31621 aaagtgcatc taaaaaaact tatcctaaac gtatttctat ccatcattct taaagaggtt
    31681 gtgacagaag tggtcagctt caagaaataa gaaggaaccg cttagtttcc agtcacaagg
    31741 aacaatttca aaaaacagct tctcatgttt gatgaatatt gtgacttgtg accagttggt
    31801 aggagaacac cgtttagtgg agaggtggtg aaagaaacgt tcagccttga gcgatgacaa
    31861 aagctctcac aaacattgcg acgagtaacc gtaggagcaa taagaggcgg taaaaacccg
    31921 tctcttattt ttcgttttct ttcactgttt cccgttcaac tagtacaggt tcatagagaa
    31981 tatttggctg tgcctgtcct ttttcaatcg ttgtgacgat ccaccgtgcg gcatcttgtc
    32041 ccaacttttc ttttggatgc gtcaatgttg tcaatttttt attccctaaa tggcttaaag
    32101 aagaatcgtc tgttcctacg atcgataggt catctggaat atgatagcca ttttctgtca
    32161 acttatctag cattaaacta gctatttgat cgttatagca aatgatgcca tctaaagctt
    32221 gttcttcttt caaccgttgt actgtttttt cagcgattgt ttctcgtgtt tccgtggtga
    32281 aagtaatgat atcttccgtc aaaaaacgca cattatactg ttcacacgcg ctgataaaac
    32341 ctttcatcct gtattttcct tgaagatcgt cgatttttgt gatgaacaac agacgctgat
    32401 gtcctttttt tatcaaatat tctgctgcca taaaaccgac tttcacatca tctaaacaaa
    32461 tataagaggc atttaactct tcataagcag cattgatcat caccatcgga atgccttgtt
    32521 ctctcaaagc aacataaagt gctaagtttg ggttatactg attacttttc gtaggctcaa
    32581 cgatcaaacc atctacccca taagcaatca ttttcttcaa acactctttc tccaattcgt
    32641 gatcgttatt cgtactggct aaaagcaatg aatacccaga ttctcgtaat tcattttcga
    32701 ttcctcggat aatcgacgga aaaatataat cagagagata tgtggtgatg acaccgatcg
    32761 tttttgttct gttcaatggt gttttttctt tcttatagct gtcatctaca tatgtgcccg
    32821 accctttttc tttacgcaga tagccctcat ttaccaacac agcgattgct tgccgaatcg
    32881 tatgtctgct gacgtcatat atttcttgca gcttgaattc tgagggaatg gttgtattta
    32941 cttcgtattc ctcacgaagg atcttttgtt ttatctcttc tgcaatatct tgatattttg
    33001 ccatttgcct tgcccctttt tatttgtacg tacatatatc ataacgagtc tgcaaggttt
    33061 attcaagcaa aaatcaaggt ttcttgtgct cataaggcat acggaacagg ttcaccaaat
    33121 tctggaaatc cttgctcatt ccacgtaaag cattgcgcgt tcgcgtgccg attaggattg
    33181 tctaacggat cccctttttg gttcttctct ggtcgagcat gatagatcaa aatatcaaag
    33241 cacccgtctt ctgaaaccgt aaacgagttg tgaccaggac caaattgctt atttttttca
    33301 gaagaaacga atactggctc cgataattta tgccatgatt cttttaataa aagattcttg
    33361 tgctcatcag cccacagcag gcccatggca tagttttcat ctgttgcact accagaatac
    33421 gtaatgaata ttttcccatt gcgagtaagg actgctggac cttcatttac tgaaaaaccc
    33481 cgtttttccc attcatactc aggtatagaa aggaggatct gcttcccctt taacgtccaa
    33541 ggattatcca tttcggatag ataaagatta gaattcccag taatttcagg atcttgttgg
    33601 gcccacacat aatatagctt tccttgagaa gagaaagttg ttccgtctaa actgaaagtt
    33661 tcccttttcg tttttatttg tcccttttct atccaatctg tattcattgg atctatatgc
    33721 tgattttcaa gtacgaacat gcggtgatga tgagattcat cacgtatagt tttatgattg
    33781 cttgcagcaa agtaaatata ccattttcca ttgatataat ggacttcagg tgcccagatc
    33841 aattcactca tcggtccttc ttcatgagcc tgccatacaa tcaggcttgc agcatgttct
    33901 aattcattca gtgactttgc ccttcttaat tcaatcactt ggtagcctgg aaccgatcct
    33961 gtaaaataat aataaccatc tgtatgttta tatacccaag gatctgcccg ttgccgaacg
    34021 ataggattat tataaagtgt cttcatcata acaaatttcc tcacattttc tttattctaa
    34081 ttacattcca agagtatttt tctatcccta catttaattc gtcatgtttt tctattgaaa
    34141 aatagtttat ttcaggtttt attttttctg gttcttcacg tgtatttttg tccaacagat
    34201 cgctactggt taatgtgtag tgctctcctt gatctagcaa attgaaatca tgttcaatcg
    34261 ttatcttttg ttgttcattt gtgcgattga tcaagaaaat agtcagctga ttgccgttat
    34321 ctacgatcac actgtccaca taaggaacat tttttatctt ttcacaatca taaacagggc
    34381 aaatgatttg ttgtttcaat acttgacctt tagcatattt cgataagtgt tgcatcacat
    34441 aataagttgg attcctccat gcttgtcctt tagcatctgt cagtattaaa ggtattgtgt
    34501 ttactaacaa tgattgacaa ctaatcttta cgcgatctgc gtgtcgtaaa atagccagcc
    34561 ctaatgaacc aaataataat gtatcttcca tagtaaagta acaccaatcg atgggactac
    34621 cgacttcaaa atccctatgt cttttttccg gttgggcatg tacattccat tcatcaaacg
    34681 ctagatacat tgttttctca cttctcaaat atccttttac ataatcacaa gtagcaacga
    34741 tttcttcaat ttgacgatcg aatcgataag cttttgcaag gtatgtcgct gtatcatcac
    34801 gctcattttt aggaggttta gtcaagtctt cttcatcata cttatcaata tagttatgca
    34861 aagctaaata atctacattc tcataagctt cttctaatgc gatacgatcc cattcagggt
    34921 acgatggtaa acgtggcgta gagcttccaa ctaagattgt ttcaattgaa tcatccacta
    34981 acttcatgac ttttgaggct tcatttgcta acctaccata ttcataagca ctcttagccc
    35041 ccacttgcca tggaccgtct agctcattcc ctaaacacca taatttaacg ccaaaaggct
    35101 ttttttgccc attttttatt cttaaatcac tccagaaggt tccttttgga aaattacaat
    35161 attctactaa attttttgct tcatcaattc cttttgttcc taaattcacc gtgaatatcg
    35221 gtttcgcttg tacatgactg atccatttaa aaaattcgtg gagtccaaaa gaatttggtt
    35281 ctatacctcg ccatgctaaa tcaatttttt ttggtcgttg ttctttccta cctacagtat
    35341 ccacccaatc ataaccagat gtaaagtttc ctccaggata gcgaatgaca cctaattcta
    35401 attcctttac ataagagagg acatcttgtc taaacccgtc accatcagct gtttcatggc
    35461 ccggttcata aatccctgta tagacgacac ttcccatatg ctcaacaaat gagccaaaca
    35521 gccgagaact gatctctcct accgtaaaat cctctctgat ttttactttc acttcttcgt
    35581 ccaacagctt tccctacctt tcatttccta tagtcaagat aatagataca agtttccata
    35641 cgccaccagc aaagataaaa ataaaaggag ggaaaagaaa tatgagcagt acaaatccta
    35701 ataacaatac gcaacaaaga atatttctca aaaaatcaaa aaatactttc catgcacttt
    35761 gttcaaaaat ttgtaaaaat gtgatatctt tgttttcaga aatcacatat atccaggata
    35821 aaagaaacat ggaataaata agtaaaacta catatccact aattgaaagt acgattgtct
    35881 gttcttctaa taattgatta acaagaaaat aaataatgat cgttaacaga ctaaggaaca
    35941 agttcttacc aaaatgttgc tttattcctt gaaagaaaaa ataaatgata tttcctgatc
    36001 cctcaccttt ccattgcgcc attacataaa aaacagcaca tgtagcaggc acgatagtaa
    36061 taacaggcaa acacacgatt acccagagaa tacctaataa gatgtaatca gaaaccacaa
    36121 tcaactgtcc ataaaattta gtctcaacta ttttcatctt cctcactctt tcattccaga
    36181 catagctgtt ccttgaacaa aatatttctg tgcaaaaata taaaccacta gtaatggcag
    36241 cattgaaata accgttccag ccattacagg tccataaaaa gaaatgttct gccctgtaaa
    36301 taaagctaac cctgctggta aagttctcat ttttgaacta cttgtcaata tcaatggata
    36361 aagcaagtca ttccaactat tcatcagcgt gaaaatccct aatgataaaa gagtaggttt
    36421 gctcaatggc aacatgattt tcaaaaaaat attaaactca cttaatccat caatgcgtgc
    36481 tgcctcttcc aaagaatctg gtaaagtaat aaaaaaggaa cgcatcatga agataccaaa
    36541 tgcagttgtc atacgaggaa tgatcaaacc tgcataagta tcaagcatgc caagcaaatt
    36601 gacttcaata aatagaggaa tcatgaagac ttggaatggg atcatcattg tcagcaaaac
    36661 gaaatagaac aaaactgatt ttcctttgaa acgcatacga gcaaatgcat accccgcaag
    36721 agaatcaaag aatacagaag tgatcactgt ccctccagca aaaatcactg tatttttgat
    36781 gtaatctaat aaagggatcg ttgtccaaac cttaatatag ttatctaggg tatagctatc
    36841 tgcaaatatt ctaggcggaa atgagataat gtctttttcg tgtttaaaag aagaaataat
    36901 cagccacaaa aaaggaaacg caataaccaa caatataact agcatactaa tcgttcgaat
    36961 gattttttta ccactaactg cttttcgact attcacagta actgtaatcg agctttctgg
    37021 tgccatacag gtttccctcc tatttcattt gtttttcttt ccgtgacatg tacacattag
    37081 tggagatagc tatagttgca acaatgataa acaaaaccga agacagtgct gatgcatagc
    37141 ccaattcata aggtgctgta aatcctcgac tgtaaatata ttgaacagct gtttctgtct
    37201 taaattgtgg gccaccagcg gttgcaacat atacttggtc aaacacttgc atcgaaccaa
    37261 tcagattcgt taaaatacaa aaacctaagg agggaacgat ctgaggtagc gtaatgttaa
    37321 aaaactgctg tcgatttgtt gcaccatcca tttgagcaga ttcatacaaa gagggagaga
    37381 ttccctgaat agcagtcaag agaatcgtca tagtaacacc aaaattcttc caaacagtca
    37441 tgatggctac cgtcggcata gcaagggttg gatcgcgaaa aaattgaggg atcggcaagc
    37501 cgacttgtga aaacaagtaa ggaatcgccc ctacattcgg atcaagcaac atagaaaaaa
    37561 taatcgcaat tgacgtcaat gaacaaataa ctggaatgta aaatgtcgaa cgacaaaaac
    37621 gattgaatct ggtattttta gataatgcta ctgctgctaa taaaccaacg ataatttgtg
    37681 ttggcgtttc gagtacggtg aaatataaag aatgaatcaa cgaattaact gctcgttcat
    37741 cactaaatat acgtgtgaaa ttttcaaaac ttacaaaatt tcttgatgta aagaaaatat
    37801 tcaaatctgt aaaactaata gcaaatgttc ctaaaagcgg tatgacagta aataataata
    37861 aaactaaaaa agatggagtt aaaaacaaat acgcagcacg atcaggacga ttccaatatt
    37921 tggtgaatcc gttcatatag tccacctcat ctttcatttt gataaaatat acaatcttgg
    37981 tttaaacgaa tctgaataag atatccgaac aatcgctgtg atttttgcaa taacacctat
    38041 ctgcaaatac taggtcgatc attcagatat ctgtatcttg ccaacagtct atcctgtata
    38101 aagttctgta ctatttttgc agtaattgat tgatggcatc atctgcatcg ttcatgagtg
    38161 tctcaggatc ttctcctgct aataattttt caatcagtgg attgatgata tcgttattga
    38221 tcgcagagat ttgggtaaaa cctggtaaat atggctctgc atagtccatt tgtttcgata
    38281 attcagatac aataggatca ttttttactt cttcatcttt tgctactgaa tccaagtagg
    38341 caggaaaacc attttcgata ctccattttt taccaatttc agttgtattc caatatttca
    38401 aaaactcata gacagcttct tttttattat tatctgtact tgccggaatg gcatatccta
    38461 cgccatctaa aatagctgtt tttttgtctg aatcactccc ttgtggtaag gtagtcacat
    38521 tataatcaat ctcgtttgct ttcaatccgt tatttaacca tggcccattg atttcaatgg
    38581 ctagttgacc agcattcatt aaattgtcca tttcggcgcc agaaatcgat tctggaccag
    38641 tcttgtctac atggatcatt ttttggatcg cttttaacgt tttaaggttc tcaggagacg
    38701 caaattcaga cttagtataa tcatcgttca ctaatttacc gccatttgct aacatccagt
    38761 tatataacgg tgcagttcca tccttattga agacaaaacc tgatacattt ttactactat
    38821 cagttaattt tggtgccatt tctgtcaatt cttcccaagt agtaggtggt tgttcagtat
    38881 ctaatccagc ttgtttaaac aaatcattat tccaatacat atacatgcct tgtacctgca
    38941 tggggacaaa atactgttta ccttctactt cacctaattc aatagccgtt tctttaaaat
    39001 cagatttatc tactccatca tattcccaga aatcgtctaa tggttgtacc gctccgtttt
    39061 ctacatactg tgcaaaatct ggataattta atgcaataaa gtcaggtgct tttttagaag
    39121 aaatcgctgt aggaagtttt tcattcaaat tggcccaagt catgatatcc atttcaattt
    39181 tgatgttttt atcatttgtt ttattaaatt cattaacgat ttcttgcaat atttcaccat
    39241 cagatgcagt aaacccattc caaaaagtca aagtcacttc tttatcagaa tcagattctg
    39301 ctgaacttcc gccaccgcaa gctcctaaaa tacctgttgc agctaccaac aaagagccaa
    39361 ttaaccattt tttacctttc attttttgtt cctccttcat ctgataaacg acatctgacc
    39421 acattccaag atagcggttt caataaacaa ttgattttcc catcttcagc ttttacattt
    39481 gttgaatgac ttggttttac ttgttcactt ttaggaagat tagttgcttt tgtatcaaat
    39541 ccactcattt cagaaaaatc aatcactccc tctaatgata attcagacaa gtgaacagtt
    39601 agattcattt cttcttctcc acggttaact gcaaatatga caagttcttt gtcatttatt
    39661 acggctatac tttctaaata cggcacttct tcaaaatctt ccgtcgcata gtttgctgaa
    39721 cgaatttgtg ggcttaacac ctctccttgt ccatacacag cagcttgcat aaatggataa
    39781 tagatcgtct gtctccaagc ttctccgttt gtttctgtcc tgattggtgc aataacgttt
    39841 accaattgag caagacaagc aatctttact ctatctgcat gtttcaataa agtgattaat
    39901 agacagccta atagtaatgc atcttcaaaa ttatagttat cttctagtaa aggaggagca
    39961 atctgccaag gcgctgcttg ctcatcttgt tccgctgaat gataccaaat attccattca
    40021 tcaaaggaca ggttgatctg cttcttacta tgtttcttcg ccttgactgt atcgcacatc
    40081 gctgtcactt cagaaatgaa ccgatccatg tctaaggaac gtgcaagata atgagaaaga
    40141 tcattttttt ggttgccata atattgatgc agagacaaat aatctatttg atcgtacgct
    40201 tgatcaagaa ctgttaactc ccaatcacca aatgtaggca tttgccgatt cgaacttccg
    40261 cataaaacca cctcaattga atcatctacg agtttcatcg ccttagccgt ttcctctgct
    40321 aaccgtccat attcttccgc tgttttatgt ccaatctgcc aaggaccgtc catttcattc
    40381 cctaaacacc atgtcttaat tccaaaaggg ctctcttgac cattcgaacg ccttaaatca
    40441 ctccaataag ttcctttagg aaaattacaa tattcgacta aattacgagc tgcatctact
    40501 ccacgagtac ccagattgac agccatattg atttcggccc ccacatgctg acaccagtta
    40561 taaaattcat gaatcccaac ttcattcgtt tcaattgtcc gccaagctaa atccaacctt
    40621 ctaggtctat ttgctatggg cccaatactg tcttcccaac gaaatcctga cacaaaattt
    40681 ccaccaggat atctaatcat gggaatgttc aattctttta ccaagtgaat gacgtcttgg
    40741 cgaaatccac ttttattagc tgatggatga tctggctgat aaatcccttc atatactgct
    40801 cgtcccatat gttcaataaa tgaactgtac aaccgcggat caactttgcc tacacgcttg
    40861 acactactaa tcaacattgt ggcttccata actgacctcc tttaatttat acatgaataa
    40921 tacacctcgt tgaaagcgtt gtcaataata taaatgttaa acttacttat tttttatcga
    40981 ttttaattaa ccaaaatatt aataatgatt aaaacaaata atttaatata acttttatta
    41041 acatatatgt taatatataa gaaaaacctt ttaagaggtg atatatatgc aactacaaat
    41101 aaacgaagaa tccttaccgg tatatgaagc attagctagt aaaacaagga tcaagatcat
    41161 ccagctactt tccaaaaaaa agatgaatgt caaagatttg gcaaaagaac ttggagtgag
    41221 cagtgctatc acgacgatgc acgtgaaaaa gctagaggaa gcaaacatca tcaaaacaga
    41281 aaaagtagga caacaaaaaa tttcgagcct tcgtgtcgat aaaatcgata ttagttttcc
    41341 agaaaaaata ttcaatgctt ttgatacgaa agaaacatcg atacctattg gtcactatac
    41401 aaattatgca attgaaccaa catgcggttt agctacgatc catgatttta tcgggaaagt
    41461 ggatgaacca cgctatttta tggatcctcg aaggatggat gcccgcattc tctggtttac
    41521 cagcggattt gtcgagtatc aggcacctaa ttttttaaat acagaggata cattggagat
    41581 gttggaagtt tctgtggaaa tttcttctga atttcctttt tcaaatgaca attggccttc
    41641 agatatcact ttcagcctaa atggtgtgga gctaggaaca tggacgagcc ctggagattt
    41701 tgctgatatc cgcggtaaat atacgcctga ttggtatcca gataatctta atcaatacgg
    41761 cttgctgaag acgatccgaa ttaccaaaca cttgacgaat atgaatggag aacccttgtc
    41821 aaacatcacg atcaacgaca tccctaaaga gcaagacact tggcatttgc gaatcgaagt
    41881 gaaagacgat gccaaacatg tggggggctg tactttattt ggtaaaggat tcggtaatta
    41941 cgatcaggat atcaaattaa aagtttacta cagctgatat ttttcttttc tattttataa
    42001 aacaaaaaca gccaatccct atgaagggac ggctgttttt gctttattgt tatttccaga
    42061 aatcatccaa aacggaaatt ggcaaatgtc gtttatgttg tgtcttgttc caccatgctt
    42121 cgattttttc ttgtgcttga gcgggaactt cttttccttc tagataatca tcaatttcat
    42181 tatatgtgac acctagtgcg acttcatctg caatcattgg tttcccatct tcaagatctg
    42241 cagtcggaat ttttgtgtat aatttttccg gtgcattcaa tgcttgaagc aattgtttcc
    42301 cttgtcgttt gtccaaacgg aataatggca aaatgtcggc tccaccatca ccaaatttcg
    42361 tgaagaatcc agtaatgttt tctgcagcat gatctgtccc taatacggct cctgctcttt
    42421 ctccagctac ggcatattgc gtaatcatac gttgacgtgc tttgatattt cctttgttaa
    42481 aatcgctgat tcccacacct gcttcgctta atacttgtac ttgtgcatca acagctggtt
    42541 tgatatttac tcgcagacta acatcgggct ggataaagtc aagagcagct tttgcatctt
    42601 cttcatctgc ttgttcacca taaggaagac gaacagcgat aaattgatag ttttcatctt
    42661 ttgtttcttc acgcatttct tccatcgtta gttgcgccaa acggcctgct aaagtagaat
    42721 cttggccgcc gctgatacct agtacaaaag ttttaagaaa cggatatttt ttcaaatatg
    42781 ctttcataaa gtcaatactt ttacgtattt cttcttttgg atcaattgtt ggtttgacac
    42841 ctaattcatt gataatttct gtctgcaggc tcatttattc tcctcctatg cttctgtatg
    42901 aagttctgct acgctttttt tcattcgttc catgatagcc attttatggt tccagcattc
    42961 agtagacaga tcgactgggt atttttgcgg attcaagtct cgtttatact cttcccatag
    43021 tgaatctaga ttttcttttg tatattcttt gatttcgttt agtgcaggta attcgtaaac
    43081 tctttttcct tcgataaaga tatcttgtaa aactgggcga gctgtaaagt ctcgaacagt
    43141 tttattgata aacgtatgca ctggatggaa catgaagata gcttcttctt ctcgagggtc
    43201 ttcatcccat aatgtcacgt agtctccttc agatttccca tcaaaatttc gagtgatgcg
    43261 ccacacttgt tttttccctg gagtagttac tttttctgca ttgcttgata acttgatcgt
    43321 atccatcatt tttccttcat catcttcgat cgatactagc ttgaatactg caccaagggc
    43381 tggctgatca taggctgtga tcaattttgt cccaacaccc catacatcaa ttttagcttt
    43441 ttgcatcttt aaattcaaaa tcgttgcttc atccaagtca ttcgaagcat agatttttgc
    43501 ttccgtaaat ccggctgcat ctaattgttc ccgaacacgc ttagatatat aggccatatc
    43561 tccactgtcg atgcgtacgc cttggaagtt gattttgtca cctaattctt tcgccacgcg
    43621 aatcgcactt ggcacacctg atttcaacgt atcataagtg tcgactaaga acacgcagtc
    43681 tttatgggtt ttagcgtagg ccataaatgc ttcataatca tttccataag attggactaa
    43741 cgaatgagca tgggtcccgc tagctggtat cccaaatatc ttcccagcac gtacattgct
    43801 tgttgcatct gctccgccga tatatgccgc tctagttccc cagactgctg catccagttc
    43861 ttgtgcccgt ctagttccaa attccaatag tggatcttct ccgatgacag atttgatacg
    43921 agctgctttt gtcgcgatca acgtttggaa attgaccata ttcaacagtg ctgtttctac
    43981 taattggcag tgcgctaaag gcccttccac ttggatcaat ggttcatttg caaaaactag
    44041 ttctccttca cgagcagaac gaactgttgc tttaaattca aaattttgta aatactccaa
    44101 aaaaccttca ggatagactt ctaatccacg caagtaagca atatcggaat cattgaacgt
    44161 cagattctct aaataattga ctaaccgttc caatcctgca aaaaccgcgt atccgtggtt
    44221 aaaaggcatt tctcgaaagt aacattcaaa aacagcatgt aaatcagctc gacctaattc
    44281 ccagtatgtc tgcatcatat tgatttgata tagatccgta tgcagcgtca aactgtcgtc
    44341 tgggtaagta ttattcatct tgtattactc ctcttggcaa gttttcattt cctacccatt
    44401 ataacaaaaa aaattaatag gaaaagaaat cttccatttt ttctgaaaaa gcacatgttt
    44461 tacggatatt t
//
LOCUS       DAYYCY010000005        42726 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000005 DAYYCY010000000
VERSION     DAYYCY010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42726
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.005"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            352..1041
                     /locus_tag="ACHAZB_000221"
     CDS             352..1041
                     /locus_tag="ACHAZB_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM8612935.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1028..2236
                     /locus_tag="ACHAZB_000222"
     CDS             1028..2236
                     /locus_tag="ACHAZB_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFM8612936.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2530..3801
                     /gene="serS"
                     /locus_tag="ACHAZB_000223"
     CDS             2530..3801
                     /gene="serS"
                     /locus_tag="ACHAZB_000223"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFM8612937.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4350..5834)
                     /gene="guaB"
                     /locus_tag="ACHAZB_000224"
     CDS             complement(4350..5834)
                     /gene="guaB"
                     /locus_tag="ACHAZB_000224"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFM8612938.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6339..7034)
                     /locus_tag="ACHAZB_000225"
     CDS             complement(6339..7034)
                     /locus_tag="ACHAZB_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HFM8612939.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7124..8224)
                     /gene="ychF"
                     /locus_tag="ACHAZB_000226"
     CDS             complement(7124..8224)
                     /gene="ychF"
                     /locus_tag="ACHAZB_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFM8612940.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8233..8424)
                     /locus_tag="ACHAZB_000227"
     CDS             complement(8233..8424)
                     /locus_tag="ACHAZB_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HFM8612941.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8445..9335)
                     /locus_tag="ACHAZB_000228"
     CDS             complement(8445..9335)
                     /locus_tag="ACHAZB_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFM8612942.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9322..10089)
                     /locus_tag="ACHAZB_000229"
     CDS             complement(9322..10089)
                     /locus_tag="ACHAZB_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFM8612943.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10101..10814)
                     /gene="rsmG"
                     /locus_tag="ACHAZB_000230"
     CDS             complement(10101..10814)
                     /gene="rsmG"
                     /locus_tag="ACHAZB_000230"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFM8612944.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11046..12947)
                     /gene="mnmG"
                     /locus_tag="ACHAZB_000231"
     CDS             complement(11046..12947)
                     /gene="mnmG"
                     /locus_tag="ACHAZB_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFM8612945.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(12964..14361)
                     /gene="mnmE"
                     /locus_tag="ACHAZB_000232"
     CDS             complement(12964..14361)
                     /gene="mnmE"
                     /locus_tag="ACHAZB_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFM8612946.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14660..15043
                     /locus_tag="ACHAZB_000233"
     CDS             14660..15043
                     /locus_tag="ACHAZB_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8612947.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15211..15969)
                     /gene="jag"
                     /locus_tag="ACHAZB_000234"
     CDS             complement(15211..15969)
                     /gene="jag"
                     /locus_tag="ACHAZB_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HFM8612948.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(15987..16805)
                     /locus_tag="ACHAZB_000235"
     CDS             complement(15987..16805)
                     /locus_tag="ACHAZB_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HFM8612949.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(16802..17158)
                     /gene="rnpA"
                     /locus_tag="ACHAZB_000236"
     CDS             complement(16802..17158)
                     /gene="rnpA"
                     /locus_tag="ACHAZB_000236"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFM8612950.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17276..17410)
                     /gene="rpmH"
                     /locus_tag="ACHAZB_000237"
     CDS             complement(17276..17410)
                     /gene="rpmH"
                     /locus_tag="ACHAZB_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFM8612951.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            17933..19267
                     /gene="dnaA"
                     /locus_tag="ACHAZB_000238"
     CDS             17933..19267
                     /gene="dnaA"
                     /locus_tag="ACHAZB_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFM8612952.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19465..20595
                     /gene="dnaN"
                     /locus_tag="ACHAZB_000239"
     CDS             19465..20595
                     /gene="dnaN"
                     /locus_tag="ACHAZB_000239"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFM8612953.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            20824..21066
                     /gene="yaaA"
                     /locus_tag="ACHAZB_000240"
     CDS             20824..21066
                     /gene="yaaA"
                     /locus_tag="ACHAZB_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HFM8612954.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21053..22177
                     /gene="recF"
                     /locus_tag="ACHAZB_000241"
     CDS             21053..22177
                     /gene="recF"
                     /locus_tag="ACHAZB_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFM8612955.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22174..24120
                     /gene="gyrB"
                     /locus_tag="ACHAZB_000242"
     CDS             22174..24120
                     /gene="gyrB"
                     /locus_tag="ACHAZB_000242"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFM8612956.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24140..26611
                     /gene="gyrA"
                     /locus_tag="ACHAZB_000243"
     CDS             24140..26611
                     /gene="gyrA"
                     /locus_tag="ACHAZB_000243"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HFM8612957.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            26847..27146
                     /gene="rpsF"
                     /locus_tag="ACHAZB_000244"
     CDS             26847..27146
                     /gene="rpsF"
                     /locus_tag="ACHAZB_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFM8612958.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27196..27708
                     /gene="ssb"
                     /locus_tag="ACHAZB_000245"
     CDS             27196..27708
                     /gene="ssb"
                     /locus_tag="ACHAZB_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFM8612959.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27734..27970
                     /gene="rpsR"
                     /locus_tag="ACHAZB_000246"
     CDS             27734..27970
                     /gene="rpsR"
                     /locus_tag="ACHAZB_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFM8612960.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28113..30086
                     /locus_tag="ACHAZB_000247"
     CDS             28113..30086
                     /locus_tag="ACHAZB_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HFM8612961.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30092..30544
                     /gene="rplI"
                     /locus_tag="ACHAZB_000248"
     CDS             30092..30544
                     /gene="rplI"
                     /locus_tag="ACHAZB_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFM8612962.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            30818..32185
                     /gene="dnaB"
                     /locus_tag="ACHAZB_000249"
     CDS             30818..32185
                     /gene="dnaB"
                     /locus_tag="ACHAZB_000249"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFM8612963.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            32965..34257
                     /locus_tag="ACHAZB_000250"
     CDS             32965..34257
                     /locus_tag="ACHAZB_000250"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFM8612964.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
     gene            34993..35856
                     /locus_tag="ACHAZB_000251"
     CDS             34993..35856
                     /locus_tag="ACHAZB_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFM8612965.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(35985..37607)
                     /locus_tag="ACHAZB_000252"
     CDS             complement(35985..37607)
                     /locus_tag="ACHAZB_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFM8612966.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            37856..38377
                     /locus_tag="ACHAZB_000253"
     CDS             37856..38377
                     /locus_tag="ACHAZB_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFM8612967.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(38563..40293)
                     /gene="spxB"
                     /locus_tag="ACHAZB_000254"
     CDS             complement(38563..40293)
                     /gene="spxB"
                     /locus_tag="ACHAZB_000254"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047112 - pyruvate oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HFM8612968.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            40458..41276
                     /locus_tag="ACHAZB_000255"
     CDS             40458..41276
                     /locus_tag="ACHAZB_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HFM8612969.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(41379..41576)
                     /locus_tag="ACHAZB_000256"
     CDS             complement(41379..41576)
                     /locus_tag="ACHAZB_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8612970.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            41745..42323
                     /locus_tag="ACHAZB_000257"
     CDS             41745..42323
                     /locus_tag="ACHAZB_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFM8612971.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            42418..42555
                     /locus_tag="ACHAZB_000258"
     CDS             42418..42555
                     /locus_tag="ACHAZB_000258"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8612972.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
BASE COUNT        12989 a         8198 c         8448 g        13091 t
ORIGIN      
        1 tttccaacat gtcaacaact tattttttat tatttaataa gttattggtg ttcttttgaa
       61 caaataataa tatatcaaca tcacatgtga aagtcaataa gaaaatgctt atttatcaac
      121 atttctagct tacatgatca ttggttagtt ctggtccttt ttcgttcgtc tttataactc
      181 cgagagacgt aaaatccttt tgttttcagt ttaatgttca tgttcttgtc ttattcacac
      241 caactagatg tctcaccttc taaaattttc cttaacaaag tgctgctttt atctatttct
      301 gctttttttt gctagaataa tagtaacaat atgactctgg aggatttatt tatgaaaatt
      361 ttgattgctg acgatgataa agaaatcgtt gaattattaa gtatctatgt tcacaatgag
      421 gggtatgaag cagtaaaggc ttatgatggg aaagaagcat tatctaaaat cagaacaatc
      481 cctgatattg aattacttat tttagatatc atgatgcctg agatggatgg gatgcaagta
      541 gtgaaagagc tacgaaaaga atcccaaata ccaatcatta tgctgacagc aaaaactact
      601 gatatggata agatcaaagg actggtcgct ggtgcagatg attatgtgac caagccattt
      661 aatccgttag aagtaatggc tagagtaaag tctttattac ggcgtacaaa aatgcagatt
      721 gccccagatc agcctgatgt attagaagta aatgcattaa tcatcaataa agactctcat
      781 gaagttaaaa caatcgatgg gaaagaaatc cagttaacag ctttggaatt cggtatcctc
      841 tacttgcttg ctagtcatcc taatcgagta tttagcgcgg atgaaatttt tgagcgtgtt
      901 tggaaacaag aaagtattgt ctctgctaaa actgtcatgg ttcacgttag tcatttaaga
      961 gataagattg aagaagcaac tggtggtgaa aaagtcattc aaaccgtttg gggagtgggc
     1021 tataaaattg acgcaagata gtaaacgagc aactgaaaaa cgccgtcgga tcaatttgac
     1081 ttcaaaagaa atcagcgaat tgttagcgga aggaatcatc acgattatcc ttttgcttct
     1141 attaaatgtt tctattctgg ttgttatcag ttcggtgatc aacagttcac cttctttgac
     1201 gaatgcgatt tgggattcaa agaatatttt tgcagaacga ttgaacactg atttgttctg
     1261 gaacggccgg aattttatta ttcccttttt ctttctatta gatattggtg ttttgtattg
     1321 gcgcttgatt cgaagatatc gccaaatgca attgcggcat atcatcagcg aacttcacta
     1381 catcgcaaac ggaaattatg atcatcgtat cccttttgaa ctgagtggtg acttaagccg
     1441 agtggtaaca agtatcaatg gattggtgga tagtaccgta gcagcgattg aagacgaacg
     1501 taaaatcgaa aaatcaaaag atgaattgat cacgaatgtc agtcatgata tccgtacccc
     1561 actcacgtcc atcattggat acctaggact gatagaagat ggccagtatc actcagaaga
     1621 agatctttta aagtatacgc atactgctta tatcaaagca aagcagatga aatctttagt
     1681 ggacgactta tttgaataca ctaaggtacg ccagcctgct gtcccggtga atttttctgc
     1741 ctttgacatg atccagttga tcgaacagtt agcagctgat tttgagttgg aagcttctaa
     1801 aaaaaatatc caaatacttg ttcaatcaaa agtagactcc cttatcatgg atggcgatac
     1861 cgaaaaactt gtacgtgtct tcaataatct tttgacgaat gcgttgaaat acggaaaagg
     1921 agcaacgaag atcgtaatcg aagtagaacg tatcggttca gaagttgttg caacagtcaa
     1981 aaataacgga gcaatgatcc cacaacaggc aatcgataat ctattcgacc gtttttatcg
     2041 tgtagaagaa tctcgttcac aagcaactgg aggaactgga cttggtttag caatcgctca
     2101 aagcattgtg gctttgcacg gcggatacat ttatgcaaaa tcagataagc aatggacttc
     2161 tttcatcatg caccttccaa tcaagaaaaa tgaaaaatta cctattgata cacaggaagt
     2221 tattgacgaa agctgaaata ttcgctattc ttattacaat tattagaata aatgaaaaac
     2281 acagaaccca gtacacttta ttctttttca gagagctgat ggcaggtgta aatcagcaaa
     2341 ggataatttg gaagttccag ttcgtttacg tgccctttcg aaataagaaa ggtcggttca
     2401 acagccgtta tgtgttcaac aagtgcagct gacgctgaac ttgggtggta ccgcgaattt
     2461 cagactgatt cgtcccaaga ataatgggat ggatcagtct ttttttattt tcaaaaagga
     2521 ggaaatcata tgttagatgt aaaaatgatg cgacaaaata ttgaagaagt acaagaaaaa
     2581 ttgatgactc ggggcgtaaa aaaagaaaca ttagctgaat tcatggaact agatgaaaat
     2641 cgtcgtaagc tactagtcaa aagtgaagaa ttaaaaaaat atcgaaatga tgtttctgct
     2701 gagatcgccc aattgaagcg tgcaaaagaa gatgcttctg ctaaaatcgc tgaaatgaaa
     2761 gaagtcggtg gcaacatcaa agcattggat actgaaattg ctgaagtgga tgaaaaattg
     2821 aaacaaattg caactacttt gccaaacctt ccccatgatt ctgtgccagt tggaacagat
     2881 gaagaggaca atgtagaaat ccgtcgttgg agcgaaccac gtacatttgc ttttgaacca
     2941 aaaccacact gggaaattgc agaaaatctt gatattttag attttgaacg tggcgcaaaa
     3001 gtagccggta gccgttttgt ctattacaaa ggattaggtg ctcgattaga acgtgctgtc
     3061 tacaacttca tgcttgatca gcatatttac gaacacggct atactgaaat gatcactcct
     3121 tatatcgtca acagtaaagc gatgtttggt acgggacaat ttccaaaatt caaagaagat
     3181 gttttccaat tggagaatac tgatctaaca ttaatcccaa cagctgaagt accattaact
     3241 aactattata atgatgaaat tttagatggt gcagatttac ctatttattt cactgcacta
     3301 agccctgctt ttcgttctga agccggaagt gctggtcggg atacacgagg actgattcgt
     3361 cttcaccaat tcaataaagt agaaatggtc aaattttcgg atgtagagca ttcttacgaa
     3421 gaattggaaa aaatgacagc tgatgcagaa gatatcttga aaaaattgca tttgccttat
     3481 cgtgtgatgg ccttatcaac tggagacatg ggcttttctg cagctaaaac ttatgactta
     3541 gaagtatgga tacctgcaca agatacttat cgtgaaatca gttcttgttc taactgtgaa
     3601 gatttccaag cacgccgcgc aatgatccgt tatcgcgata atgatggaaa aatccactat
     3661 actcatactc tgaatggttc aggacttgca gttggccgta cagtagctgc cattttggaa
     3721 aactaccaaa atgaagatgg ttctgtcaca gttccagaag cgctcgttcc ttatatgggg
     3781 aatctgaaag ttattaaata aagaggctgt gaaaaagccg cttagtttcg agtattaaga
     3841 aaaattttgt gaaaatggct ttccacattt ttgcataaat tggacttaat accgagaaac
     3901 tggcttttga acaccattta tcgagaggtt gtgaaatcag cgaattaacc tgagtattag
     3961 agaaaaaaat ggaaaaatag tccctcatat tttaccgttt gttgaactaa taccgagggt
     4021 cagctgattg aacaccgttt agtggagagg tcgtaagcct accccccagc tcctagttac
     4081 aaggacaaat tcaaaaaaca gctcttcata ttttatgaat gttgtacttg tgattcggag
     4141 gtggcaaggg aacactgttt attcaaaaag aggctgggac agaagcgttt aactccaaga
     4201 aataagaaga aattcacgaa aattgctttt caaatttttg tgaatttctt cttatttccg
     4261 aaggagttgc ttctacttcc gccgtttata cgtgtttaga gcgtgagaca aaagtgttct
     4321 ttacttttgt cccacgctct ttaatgtttt taagattcaa ctgaatagtt tggtgcttct
     4381 tttgtgattt gtacatcatg agggtgtgat tcttttaatc cactgccgct catttggata
     4441 aattgcgctt catcacgtaa ttgctgtaga ttggctgccc caacataacc cattcctgaa
     4501 cgcaaaccgc cgatcatttg gaaaataata tctgcaacac ttcctttata agcaacccga
     4561 ccttcgattc cttctggtac taatttattt gcttcattga cactaccttg gaagtaacgg
     4621 tcttttgaac ctttttccat cgcgcctaat gagcccatac ctcggtatgt tttgaatcga
     4681 cgtccttgga agatttcgaa ttcacctggt gattcatctg tccctgccaa catacttcct
     4741 agcatcactg catgtccgcc ggcagctaat gctttgacga tgtcaccaga atatttgatc
     4801 cctccgtctg caataatcgc tttgccatat tgacgagcaa ctgatgctgc atcataaata
     4861 gctgtgagct gaggtacacc tacaccggct accacacgtg tcgtacaaat cgatcctggt
     4921 ccgataccaa cttttacgac atctacacct gcatcatata aagcttttgt cgcttcagcc
     4981 gtcgcaacgt ttcctgcaat caatgttgca tctgcaaaag ttgaacggat ttcttgaatt
     5041 tttcggatca cacctgcact atgtccatga gctgtatcga tgatgattgc atctgcccct
     5101 gcttctaata aagcattcgc acgctcaaat gtatcgcttg ttacaccaac ggctgctgct
     5161 acaagcaatc gaccatgttc atcttttgct gcatttggaa attcgatcac tttctcgatg
     5221 tctttgatcg tgatcaatcc gcttaaacga ccttcgttat ctacgattgg taatttttcg
     5281 attttatgtt tttgcaaaat cttttctgca tcttttagag aggtacctac tggggcagtc
     5341 actaagttgt cttttgtcat cacatcagca atcggcatgt gataatctgt tacaaaacgc
     5401 atgtcgcggt ttgtaattat ccctaccaat tttctgtttt caagtgtttc aacgattggc
     5461 acaccactga tacgatattt gctcatcaat tcttctgcgt ctgctacaag atgctgtggc
     5521 gttaagaaga acggatcaat gattacgcca ctttcagaac gtttgacttt tcttacttca
     5581 tccgcttgct ggctgatcgt catgttttta tggatcacac ctagtccgcc ttgtcttgcc
     5641 atagcaatgg ccatcttgct gtcggtaaca gtatccatac ttgcactgat gattggaata
     5701 ttcaacttaa tatttttagc taattgtacg ctcatatcca cttcgtttgg taacacgtga
     5761 ctttctgctg gtatcaataa gacatcatca aaagtaaatc cttttttcgt gaactttgtt
     5821 tcccagttgg acatttttag taccactcct ctaatttagt ttatctatta aaataattga
     5881 ttttttcatt taagtcaatg atctacttgg aatcaagtta gctttctcat agaataccaa
     5941 tataaagcgt ttacatctgt gtatattaaa agagattgta aaaaagctgt cctgcatcaa
     6001 gtaataagaa cagccaatca aaaacagctt ctcatgtttt tgaattggtt gggtttatta
     6061 ccgcagatgc aaacttttga acaccgttta gtggagaggc tgtgaaaaag ctgcttagtt
     6121 tcgagtagta agaaaaattt tgtgaaaatg gctttccaca tttttgcata aattggactt
     6181 aataccgaga aactggcttt tgaacaccgt ttatcgtaaa aagggactgt gacaagactt
     6241 ttgtcacagt cccttactat tatttaacta tggataaaca aaatgaggtt ctttactacc
     6301 cctagatatc tagcaaaata gctgatcttt ttgctagttt atcttcttgg gacggataaa
     6361 cttccaataa tcccgtattt tttcttcaag tagtaacgga ttgctaaagc agttgccccg
     6421 ataatgataa ctactactgg atctaatact ggattgataa ctggtggcaa tagtgttgtc
     6481 gcagaaaaca gtacgaccca aagaagtgtc gttccaccta ggataagcaa tgtcttaccc
     6541 attcctggtt tcttgcttcg atctgctccg ggatattcat attgatagat gtatttgtac
     6601 atcatataaa agacccagcc accgattgca gaacctagaa cgagtgtaga aaagccataa
     6661 gctggcgttg ttccacgtga aaacaatccg gtaatcgcaa tcatgatact taatagacca
     6721 agcaacagca acgtattatc taaccacatc aataaagggg tggatgaatt ttttacttct
     6781 gctggcttat tgatgattgc ttccgttctt tcagagactg tcccaaacaa ttgacgggct
     6841 gttcttccgc ctttttgttc tgcaactaaa gtaggcagaa tgtcatgaag ggcttttact
     6901 ttttcttctt cagacaaatt cgctgcttct aaagattttt tcaaatcaaa aatgtactgt
     6961 tgatttcttt tggttaattt tgtttctaaa ctattgtttt cttttacgat tgctcgtaat
     7021 tgttctggtt ccattatcta atagtcctcc ttagccgttc cttttctcaa cggtcgtcac
     7081 aaaagacatt ctaccatatt ctacagctag tttgatagcc cttttacaca ttaaagcgga
     7141 ataacattac gtcgccgtct tgtacgacat aatcttttcc ttctaatcga acacgtccag
     7201 cttcttttgc tgcatgcatg tttccgtaat gatccaagtc ttcaaaagag acagtttctg
     7261 cacggataaa tccgcgttcg aaatctgaat ggatgattcc tgctgcttgt ggtgctttga
     7321 ttcctttttt gaatgtccaa gcacgtactt cttgttcccc agcagtaaag taagtagcta
     7381 agcctaacaa atcataagca gcacggatca actgatccaa acctgattct tcgattccta
     7441 aagcttctaa aaattccgct ttgtcttctt catctaattc tgcgatttct tcttctgcac
     7501 gagcactgac cacgatcact tctgcatttt cttctgcagc aaagttgcgc acttcttgta
     7561 cataatgatt cgcgtctgca tctgctacgt catcttctga gacattggct acataaagaa
     7621 ttggttttgt cgtcaataaa aatagtgatt taacgatttt ttgttcttct tcagtaaatt
     7681 cgatcgttcg ggcagatttt ccttcttcca gtacaggttt gattttatcc agtacagcta
     7741 gttctgccac tgcatcttta tctttggttt tagcaacttt tgctacacga gtatagcgtt
     7801 tgttgataga ttctaagtct gctaacacta gttccaagtt gatcgtatca atatcttcta
     7861 gtggatcaac acgtccttct acgtgagtaa tattatcatc atcaaaacag cgaacaacgt
     7921 gacaaatagc gtctacttgg cggatatggc ttaagaattg atttcccaaa ccttctcctt
     7981 tacttgcgcc ttttacgatt ccagcaatat ctgtaaattc aaaagtcgtc ggaacagttt
     8041 ttttcggatg tacaagttct gttaagcgtt gcaaacgcca gtcaggtact tctaccatcc
     8101 ctacgttggg gtcgatcgtc gcaaagggat agtttgctgc ttctgctcct gcttttgtga
     8161 tcgcattaaa caaagttgat tttcctacgt tgggcaaccc aacaattcca gcagtcaatg
     8221 ccataaattt attcactctt tctctgcatt tttttcaatt acttttttta atttcttttc
     8281 aaaatctcga cgggtcatca tgacgatatg tccgcaattc atacatctga ttttgatatc
     8341 tgcgcccatt cgaatgactt cccagcgatt cgtctggcaa gcgtgaggtt ttttcatttc
     8401 aacgatatcg tgcaagtcgt acataaaaat agcctccttt ttgtttattc ttcatcaaat
     8461 tgaatattca agatatcaag aattcgagtc aaatcttttt gagaaagata ttcaatctcg
     8521 atcttacctt tcccattttt ttcttggata gctacacttg taccaaattt atccatcaaa
     8581 cgatcttcgc tttctcggat ataatatggt ttttcctttg ctagccttgg gatttttttc
     8641 ttttcttttc catcattttg atttaactca gaaacaagct gttccagttg gcggacagtt
     8701 aaattttcag ttactgcacg attggctaat ttcaagatca atgttttatt ttttaaacct
     8761 aacagcgtac gggcttgtcc catagataaa cgctgatctt gtaccatttc cttgacaaga
     8821 tccggtaaag aaagcaagcg caaataattg gcgatataag gtctgctctt tcctaaacga
     8881 ctagctactt ctgcttgtgt tagtttcagg tttttcatca gcatctcata tgcttctgct
     8941 tcttctaacg gattcagatc ttctcgctgc aagttttcca gcactgctac ttgcatcatc
     9001 gcttcttcgt caaagtctct aacgatagca gggatcgttt ctttttcagc tagtttggac
     9061 gctcgaaaac gtcgctcccc tgcaataatc tcatatcctt ttactgcgga acggcgtacg
     9121 atgatgggtt gaaagacacc tgattgttca atagaattag ctagttcctg caaagatact
     9181 tcttcaaatg ttttacgggg ctgatacgga tttggtcgca gctcattcaa tggtagctgg
     9241 acaacggtat catcttttac atccaccgtc tctaagtttt ctaaatcttg aaacagtgca
     9301 tcgattcctc ggcctaatcc tttacttttc ttcacgactt accacttcct ttgccaatgc
     9361 ttggtagact tcagctcctc gagaacgtgg atcgtagtcg atgatcggtt ttccatgact
     9421 aggtgcttct gataaacgaa tatttcttgg aatgatcgtt tcgtaaactt tttcgcggaa
     9481 gtatttacgt acttcttcta cgacttcatt tcccagattt gttcgtgcat catacattgt
     9541 cagtaagact ccctcgatct ctaattctgg attaaagtgt ttttggacta aacgaaccgt
     9601 attcagtaat tggcttaatc cttccaatgc gtaatactca cactgaacag gtatcaaaat
     9661 cgaatcactt gctgtaaatg aattgattgt cagatggcca agagaaggcg ggcaatcgat
     9721 caaaatataa tcatactgac tgcttacttc tgctaaagat ccttttaatc ttgattctct
     9781 tgccatcatt gacgtcaatt cgatctctgc tcctgctaat tggagagtcg caggcacaat
     9841 actcaaattt tcgtgttcag tgatcaaagt tgcctcatca attggcaatt cattgactag
     9901 aacatcatag atgtctcttg tcacatcagg ttttctgatc ccgacaccac tggtagcatt
     9961 tccttgtgca tccatatcca ctagcaatac ttttttgcca aggctagcaa gacaagcacc
    10021 tagattcacc gtcgtcgttg ttttgccgac gccacctttt tggttcgcaa cagatattat
    10081 ttgtgccatc tcttctcctc ctatttgatt ggctgcttat taggcagtcc gggctttctt
    10141 gggtatttct ttggggttgg tttctttttt tgaataacga tgatatgccg ttgatcagag
    10201 acattcggca aggtaaccgc ctcttctttg aggaatttac cacctaataa agcaatcgct
    10261 tgttttgctt ctgtcaattc ctcttcactt tttgctgctt tcaatgcata aaaatagcct
    10321 tcttgtttag ccaagggcaa acacaattcc gctaacacat tcaaacgagc aaccgcacga
    10381 gcagtgacaa ggtcaaatga tgcacgaaag tgaggatttt gcccaaatgt ttctgcccgg
    10441 tcatgatata aggaagcatc gattcctaat aatcgggtta actcagataa gaaggttatt
    10501 cgtttattca gagaatcaac gatcgttatc tgcagttctg gaaaggcaat ttttaaaggt
    10561 atgctaggaa accctgcccc cgaaccaaca tcgcataaag ataaaggttg cgtaaagtct
    10621 acttcaaatg ctaatgtaag tgaatcgtaa aaatgcttca gatatacgtc tttttcatcc
    10681 gtaatagctg taaggttcat cttttcgttc cattcgacta atgtccgata ataaatagcg
    10741 aattgattca tttgatgatc ggtaagctct atcccttttt ctgccaagga ttggcgaaat
    10801 tcttctggta tcataaatat tcctttcatt gtgaaacagc aattgtttca cagattctct
    10861 ccctacttta acgcaaaatc tctagacatg caatccttcc atatggattc ttaaaaattt
    10921 gaaaagtaag aaaagccgat agactttttc tttctagtat gactcataaa atatacacag
    10981 aagagagaac tagctttgag caatttctgc aaaaactagt tctctcttct atttattatt
    11041 acgtattacg ctggttcact gactttcgca atcttccctt gttcgatata gaccattagg
    11101 atacttaggt ctgcagggtt tacgccactg attcggctag cttgggcaat tgtttctggt
    11161 tggattttct gcaatttttg ttttgcttct gttgctaatc cgttgattgc tgcgtagtcg
    11221 atgttttctg gaatcttttt cgcttccatg cgttttaatt tttctacttt ttccatggct
    11281 tttttgatgt agccttcata tttgatttgg atttctactt gttcgatttc ttttcgtgtc
    11341 aattcttcat tttctggaat aaaccgtaga agttctgcat aagtaatctc aggacgtctt
    11401 aaaaagtcgc ttgctagtac accatctttt aatggtgccg cattaatcgt tgctaaaaag
    11461 tcttggactt cttctgttgg tttgattcgt acattcatca aacgtttgat ttcttgttca
    11521 actgcttctt ttttcttcaa ataattcttg tattgatctt cttttactaa gcctacacgg
    11581 tgacccattt ctgttaatcg caaatctgca ttatcatgac gtaaaagcag acggtattca
    11641 gcacgtgaag tcagcaaacg ataaggttcg ttcgttcctt ttgttaccaa atcatcgatc
    11701 atgacaccga tgtaaccgtc acttcgtttg atcactaaag gttcttttcc ctgaactttc
    11761 aaagctgcat tgataccggc aatcagccct tgtccagccg cttcttcata accgcttgta
    11821 ccatttgttt gtccagctgt ataaagattt tcaacgactt tagtttctaa tgtcggacgg
    11881 agctggtgtg gctctactac gtcatattcg atagcatatc ctgtacgcat catttctact
    11941 ttttctaatc cagcaataga atgaagcatg ttgacttgaa catcctctgg taaagaagta
    12001 gataaacctt gaacatagac ttcttccgta tttaagcctt ctggttccaa aaataattga
    12061 tgacgtggtt tgtctgcaaa acgcacgatc ttatcttcga tggatggaca atagcgagcg
    12121 cctacccctt caacgatccc tgtaaacatt ggggcacgat gtaagttgtc tcgaataatt
    12181 tggtgagtcg tctcacctgt ataggtcagc cagcaagatt cctgatctag tttataggca
    12241 ctatctggtg tgctgaagct aaaatgattc ggctctttgt caccaggctg ctcttctgta
    12301 acagaatagt cgatcgtact tgattttact cgaggcggtg ttcctgtttt gaaccgttcg
    12361 atagcaaagc caagttcttt tagatgattc gctagtccaa tagaaggttg agagttattc
    12421 ggacctgatg aatatttcaa ttcaccgata atgatttctc cgcgaagtgc tgttccggct
    12481 gtaataacaa ctgctttggc ccggtagctc gctcctgtgg acgtcactac tccatgacaa
    12541 acaccatctt cgacgatcag ttcttcgacg attccttgtc ttagcgtcaa atttggttct
    12601 ttttcgatcg tatgtttcat ttctgttgaa taagcatgtt tgtctgcttg tgctcgcaat
    12661 gctcggacag ctgggccttt tcctgtgttc aacatacgca tttggatata ggttttatcg
    12721 atattcttac ccatttctcc gcctaacgca tcgatttctc gaacgacaac gcctttagct
    12781 ggtccgccta cagaaggatt acatggcatg aatgctacca tgtccaaatt tattgtcagt
    12841 aataatgttt ttgatcccat acgtgcagca gcaagtgcag cttctgaacc ggcgtgtcct
    12901 gcgccaacaa cgatcacgtc atacgcatct gcttcataat gttccaatcg tttgttcctc
    12961 cttttatttt cctaaacaaa attgactgaa taattgagtg attagctcat cttgtacact
    13021 gtcgccgaca acttctccta gatagtccca gcaacgcgtc atatctattt gaacgagatc
    13081 cacaggcatt cctgaatcaa tacctgcaat gacttcttca agtgagaggg aagctttttc
    13141 taacaacgcg atatggcggg tattagaaac ataagtagcg tctctttctc ctgtttctcc
    13201 gctgaagaat aaatcagaaa tagctgattc caaacgatct agtccgtcgt ttttagctac
    13261 ggaaattgaa aaaactggtt cattttcgat caatttcttc aatttttctt gttccagctg
    13321 tgctggcaaa tctgttttat ttagaagaat gatccgtttc aatccacttg tcgcttccaa
    13381 taattgttca tcttctgctg tcagtggttc gctttggttt agaacaagta agatcaagtc
    13441 tgcttctgct aatgcttttc ggcttctttc gaccccgatc cgttccacca catcttctgt
    13501 ttcccgaatt ccagctgtat cgattagctt gagcggtacc ccccgaacat tgacatactc
    13561 ttcgattaca tctcgagtcg ttcctgcaat atccgtaacg attgcttttt cttctcttaa
    13621 caaatggttt aacaaacttg atttgccgac atttggccgg ccgatgattg ccgtacttaa
    13681 cccttcccgc agaaccttcc cttgcttgga tgtagcaagt agcgcttgga ttctttgttg
    13741 gatcatctgc gctttttcaa gcaataactt cgtagtcaat tcttcgacat catcatattc
    13801 tggataatca atatttactt ctacttgtgc taatgtctct aaaatctctt gtctcaatga
    13861 gcggatcaaa gcagacagat tgccgtctaa ctgattaaga gctagtccca ttgctttgtc
    13921 tgtttttgcc cgaatcaaat ccatcacagc ttctgcctgt gaaaggtcaa cacgaccatt
    13981 caagaatgct cgttttgtga attcgcctgg ctcagctaat cttgcacctt ctcttaacaa
    14041 tagctgcaaa atttgattga ctacaacgat cccaccgtga caattgattt ctactacatc
    14101 ttcccgcgta aatgtctttg gtgctctcat cacggagacc atgacttcat cgacaagctg
    14161 attgctcttg ggatcaacga tatgtccata atggatcgta tgacttggta cttctgataa
    14221 tcgtttgttt ccagattgat aaactttatc tgctagttgc acagcttgat cgccacttaa
    14281 acgaacgata ctgattgcac cttctccagg tggggtagaa attgccgcaa tcgtatcaaa
    14341 ttctaaagta atttcagcca ttttttcact tcctttttca ttttgcgcac aaaaaaagtg
    14401 cccactccac cctttatgca caataaacgg tgaacttcgc actttgactg cttgttcttg
    14461 attaaattct ggtcatagat tatcacactt gctataagga atcaagtgtt taaaacaatt
    14521 agatgaagtt gagcctgttc tgcttccatt accttatatt taattgtcct tggatgtctc
    14581 tttgcgttct ttggtttatt cgaagagaag atactgtata aacaaggctt tatctttctt
    14641 aaaaaaaacg gtacactaaa tggtagcgct atctaaacta tacacttttg aaaggagaaa
    14701 atatgtgagc ccgttcagat ctgctgcatt ggaagaagaa agtgaccctc ggatcaacga
    14761 tgtcgtttta tctacaggga atatcaacca gaaatatatc gtccgcgata tcgtttttgt
    14821 tgtggaatgc ttagaagctg atttgtttga cccatctttt cccaaagatg accttttctt
    14881 aacaacgaag aggaaattaa agacgaaggt acttgaatat ggtgcgaatg cagtaatcaa
    14941 ctgtcatttt gagtatcagc aaaaaaaacg cgacggaaag tattttctgg aactttttgc
    15001 ttatggaaca gtgatccaat ttatccaaac aacgattggt taaatgaaac agacaaaaaa
    15061 agcggatcaa aactgaagac agtttcgatc cgcttttttc tatcaaagtt agtgactact
    15121 tcatcgtata ctctgataag aatgatttct agtaaaatat acttggtttg aaaaactttg
    15181 tcaacgtgat ttattcaaaa agtaatggaa tcaataatat tttttagcag gttctactac
    15241 taaataacga taaggttcct caccttccga atgtgttttt acatgttcat ctttgcttaa
    15301 tgcagcatgg atttgtttac gttcaaatgc aggcattggt tctaagaaaa caggctgccc
    15361 tgtccgtttt actttttcag ccgtgcgatc agctaaacgc tgaatgatct cttggcgctt
    15421 ttcacggtaa ttgcctacat tgactactac agacagctta tttcttgcta aacgatggat
    15481 gaagacttgt gccagatatt gcaatgcatt cagtgttttt ccatgtttcc caatcagtat
    15541 gccttgcttt tgtgtttcta aatggaaaac aaccatatta tttgattctc tttccatttt
    15601 gacaagagct ggagcatcaa gctgttttga aatttcagta agataaactg ccaattccgt
    15661 taaagctgct tcatcaccta gctcacttac ttcttctgtt ttttcttcaa gaatcgtttc
    15721 gttgactacc tcgatctcag ctgttggttc ttcttccaaa atttcttcta cagtctgttc
    15781 tactgtttca gaaacttttt cgctgatact tggctggatc gaaagccgtg cattctttcg
    15841 acccattcct aggaaacctt tttttccttc ttccagtatt tcgatttcga cttggtcttt
    15901 ggccaaaccc aaatcagcca gacctagttg gacagcttcg tctattgttg cagcttcata
    15961 aatcggcatt agcattacct cccctattat ttccgttttt tcttaggatt cattgctttc
    16021 ttaagagcac gttctctttc gcggattctc cgtgcttctt cttcacgctc ttttcgaatt
    16081 ttgaaagggt tgttgatcag cagcgtttgt acaacttgga aggcattgga aactacccag
    16141 tataatgaaa gaccgctggc taaattgaca cccatcacta aaatcattac aggcatcacg
    16201 aaattcatga tcttcattga tgcattcgat tcaatttgac tcatgctaga taaatacgta
    16261 cttgcaaaag taaagattgc tgctaaaatc ggcaaaataa atgttggatc cgcgttccct
    16321 aagttcaacc ataagaaatg cccttgtttc aactctggta cacgtgaaat agattgccat
    16381 aacgccatca agattggcat ttgtactaat aaaggaagac aaccagcata tggattgacg
    16441 ccattttctg catataaacg ctgctgctct tctcttaact tactttgtgt ttccggatct
    16501 cttgaactat attgttgctg cagtgctttt aatttcggtt gtaattcttg cgttttacgc
    16561 atgctctttg tttggaaatg catcaacggc aataggatca cgcggatgat cagtgtaaat
    16621 aaaatgatcc ctatccctgc attaccaaat gacaacgcct tgatcgcctc agcaaagtaa
    16681 taaacaatat aacgatccca aattcctgta ctctgtgcgc tgacttcccc agttccgcaa
    16741 ccggataaga cgatgaccaa actaacgata ccggccatca aaagtaaacg tttgtatttc
    16801 ttcacttact cttttcctct ctgtcgatta tttttgcaag ttttaagacg tggactaaat
    16861 tggagcgaac ctcatcataa gtcaacccct tcacacttgg tctagcaatc acaataaaat
    16921 ccaactccgg atcaatttct tcttttatag aataaactgc tgctcggatt tttcgtttga
    16981 cttcattgcg gtttaccgcg ttgccgacct tttttcctac agataaacca actcgaaaat
    17041 gaggctgtcc cggtttttcc aaacggtaaa cgacgaattt gcggttagca aaagaatctc
    17101 ccgtatggaa cacggcttga aactctcgtt ctttttttac tcgatacgct tttctcatta
    17161 gttttctttc cttttctatc tctcacttac gctcataatc ataccataat tacgattgtc
    17221 ctttcatcat ttccctaaaa aagagtataa aaaaaaccac tgaaacccca gtggcctaag
    17281 ctgaaagaac ttttcttcct ttgcgacgac ggctagctaa cacacgacgt ccatttttag
    17341 tactcatacg tttacggaat ccgtgaactt tttgacgttt acgtttattt ggttggtatg
    17401 ttcttttcat atattccacc tccaaaatgt tcttatctat tttataaaca cagacatact
    17461 ttgatagtat aacgacaaag tagtgatttt gtcaatataa aatagaagat cgattgttaa
    17521 aacttatcca caagattatt tttttctatg ggaatcctat gtggaaaaag ttatgcacag
    17581 ggattatcca cagataataa aaggctggtt ttacacagaa aacacacgtg taaaaaaagt
    17641 aatccacaat caggcgattt cctgtgtatt ccgtctccta actattggta tatctgtatt
    17701 ttttatccac agacgcaaga ggaaatctat tggtttttac gagttttcca cctgtgcatt
    17761 agtggtgtgg aaaacttttt ttgacaactg tgtttttctc ttttttcttt ttgtcgattg
    17821 tgtggataat tgtaattaaa aaattttttt cttcggtttc tgtggaaaac tattgaaaaa
    17881 aatgctaaat tagtactagt tattcacttt ttaatgaagg aggaggcctt atatggtatc
    17941 cctcgatgct ttatggaatg aattaaaagc aacataccaa aaggatttat cgcctgctag
    18001 ctataataca tggatcgaaa cagcgaaccc tcgaaccctt gatcaaaacc agctcgttgt
    18061 tgaggttcct agcaagattc ataaagaata ttgggaaaaa aacctggcga caaaaatcgt
    18121 tgaagtcggt tatatgttat caggaaatga gattatccct cgctttatta ctggcgaaga
    18181 agcagagcaa gaagaagtta tagaagaaaa aaatccaaaa gttgtggcac ctagtccgct
    18241 aaaaaaagcc atgctgaacc ctaaatatac ctttgatacg tttgtcattg gtaaaggaaa
    18301 ccagatggct catgcagcag cgctcgttgt agcagaagat ccaggttcta tttataaccc
    18361 gctcttcttt tatggaggcg tgggacttgg taagactcac ttgatgcatg caattggtca
    18421 tcaaatgctg caaagtcagc ctaatgccaa agtaaaatat gtaagcagtg aaacttttgc
    18481 taatgatttc atcaattcga tccaaaacaa aacagcagaa gagttccgtc aagaatatcg
    18541 aaatgttgat ttactgctag tggatgacat tcaatttttt gcagaaaaag aagctacgca
    18601 agaagagttt ttccatactt tcaatgcctt gtacaatgaa gggaaacaaa tcgtactaac
    18661 cagtgatcgg ttgccaaacg agattccgaa actgcaagaa cgccttgttt ctcgctttgc
    18721 atgggggctg tctgttgata ttacaccgcc ggatcttgaa acaagaacag cgattttacg
    18781 taaaaaagcg gcggctgaac gtctagagat cccagatgac acactaagct acatcgcagg
    18841 acaaattgat tctaacattc gtgaattaga aggtgcgctt gttcgagtgc aagcttttgc
    18901 tgcgatgaat agtgaagata tttcgactag tctagctgca gacgcactga aaacgttaaa
    18961 atcaggaaaa ggacatcctc aattgtcaat cctgcagatc caagaagaag tggcaaagta
    19021 ttatcacatc caattgaaag atctcaaagg aaagaaacgc gtcaaatcta ttgttgttcc
    19081 tagacaaatc gctatgtatt tggcacgtga gctcacagat aattctttac cgaaaatcgg
    19141 agctgaattc ggtggcaagg atcatacaac ggttatccat gctcatgaaa aaatccagca
    19201 gctgatggat tctagtgtca gtatgcaaaa tgaagtatca gaaatcaaaa actttttact
    19261 aaattaattt gtcgattcta tcaactgaaa atagcatgtg gataaaagaa aaaaacgttc
    19321 aaaagttata cacacttttt ccacagctgt tttttcgttt ctctcttacg ttttttgagt
    19381 tttccacaga atcaacatgc cctattactt ttattattct ttttaactaa taataaataa
    19441 acaaacattc aaaggagata catcatgaaa gttactttaa accgagctag ctttatgcag
    19501 gaattgcaaa ctgttcaacg agctatttca agcaaaacca cgatccctat tttgacaggt
    19561 gtaaaaatca cactgacaca agaaggtttg actttgacgg ggagcaacgc tgatatatca
    19621 attgaaactt ttttgtctgt tgaaaacgaa aaagcaaata tgcaaatcga atctactggt
    19681 tccattgttt tacaagcacg tttctttagc gaaatcattc ggagacttcc tgaagaaaca
    19741 tttactttag aagttttaga aaataaacaa gtagcgatca cttctggaaa agcgaatttt
    19801 atcgtaaatg gattagatgc agataactat cctcatcttc ctgttgtcga aagccataac
    19861 cagatgaaat tacctgtaca cgtattgact aaactaatca acgaaacagt ttttgctgtc
    19921 tcacaacatg agagtcgtcc aatcttgaca ggtgtgcatt tcattttatc tgataattct
    19981 ttattagctg tagctactga ttctcaccgt ctaagtcaac gcgtgattcc agtagaacaa
    20041 gcggctgatc attttgatat tgttattcct ggaaaaagtt tgatcgaatt atctcgctca
    20101 ttaacaaatg aagaagaaat cgtcgaaatc agcattatgg aaaaccaagt gttgttcaaa
    20161 acagaaacga tgtatttcta ttcccgtttg ctagaaggaa actatccaga taccaatcgt
    20221 ttgattcctt caagttttaa tacagaggtt gaattttctg ttcctagctt tttagcggcg
    20281 atcgaacggg cttctttact ttctcatgaa ggccgtaaca acatcgttcg tttatctatt
    20341 cgtccagatg cggttgtttt atacggaaat tcgccagaaa tcgggaaagt agaagaaagc
    20401 ttgagttata cagcaagcag tggcgatcca ttagatattt ctttcaatcc ggattatatg
    20461 aaagcagcgt tgcgtgcatt tggagatatg agcatcaaag tgaaatttat ctctgctatt
    20521 cgtccattta cattggaacc aacggaagat ggcgttcaat ttattcagct gattactcct
    20581 gtacgtacaa attaacaatg attttcaatg ttcttgaaaa tttaattgaa aagaaataga
    20641 aaaaataaaa ttttaaacag aaggccctaa aatctctttt aaggctttct gtttattttt
    20701 gtttaaatca ccttttttag attaaatcgt cttatatgga aaaatatatg gataaatttg
    20761 ttttattgcg aaaaaaaagg tataatagag tatactttta ataatgaaaa tgagggatgg
    20821 gcattgaaac aacaagtcat tctaaatacg gaattcatga cgttgggcca aatgttgaaa
    20881 gaagtcacag ttatcggtag tggcggacaa gcaaaatggt atttggcaga aaacactgtg
    20941 ttagtcgatg gcgaaccaga aaatcgtcgt ggacgcaaac tttatcccgg catgatggtt
    21001 gaagttccag aagtaggaac tttttttatg gtaaaaaaag gaaacgacga taatgaggct
    21061 gaataagctg tatttaaaaa attatcgaaa ttacgaagag ctgaacatcg aattttccaa
    21121 gaacctcgtc atctttttag gagaaaatgc gcaaggaaaa acgaatcttt tggaaagtat
    21181 ctacgtatta gctatgacgc gaagtcatcg gacaagtagt gagcaggaat tgattggctg
    21241 gactgacgat caggcgatga ttcaaggaga gatcacaaaa ggttcatcca ctcttccatt
    21301 agaaatctta ttatctaaaa aaggccgaaa aaccaagatc aaccatatcg aacagaaaaa
    21361 gttaagtact tacgtgggac aaatgaatgt cattttattt gcaccagaag atctttcttt
    21421 agtaaaaggc agcccgcaga ccaggcggaa gtttttagat atggaattag gccagatcaa
    21481 tccaatctat ctttatgatc ttgtccaata ccaagcaatt ttgaaacaaa gaaaccagta
    21541 cttgaagcaa ttgaatgaaa aaaaacaaac tgatttgctt tatttggata ttttgtccga
    21601 acaactagca gcgtttggca gcaaagtatt gaaagcaaga gcacagttcg tcaagcgatt
    21661 ggagttttgg gctaattctt tgcatcagca aatcacccat caaaaagagc agctggaaat
    21721 cgagtatctc accgcagtcg attctttaga aacacatacg caagagcaaa tccaagaaca
    21781 atttctcgca cttttgaacc aaaataaaaa gaaagacctt tttcggggaa caacgacagt
    21841 tggtcctcat cgtgacgatc tttccttttt tattaatcag aaaaatgtgc agacttatgg
    21901 atcgcaagga cagcaaagaa caactgcact aagtgtcaaa ttagcagaaa tcgatttgat
    21961 taaagaagag acaggggaat atccaatcct tttgttagat gatgtgatga gcgagctgga
    22021 tgataatcgt caactccatc tgctagaaac aatcgaagga aaagttcaaa cgttcttaac
    22081 aacaacgaca cttgaccatg taaaaaataa aatgactgtt gaacctgaga ttttttatgt
    22141 tcaacaaggg aaactaaaag gagaagaaac tgaatgacag aagaaagaag tttagtagaa
    22201 cgcgctaaag agtatgatgc cagtcagatt caagtacttg aaggacttga agccgttcgt
    22261 aaacgacctg gcatgtatat cggttcgact agttctgagg gtctgcatca ccttgtttgg
    22321 gaaattgtcg ataattcgat cgatgaagta ttagcaggtt ttgcaacgaa aatccatgtg
    22381 attattgaaa aagacaacag tatcaccgtt attgacgacg gtcgggggat acctgtagat
    22441 atccaggcaa aaactggacg tcctgcagtt gaaacggtct ttactgtttt acatgctgga
    22501 ggaaaattcg gcggtggtgg gtacaaagtt tctggtggac ttcacggagt aggatcttct
    22561 gttgttaacg cgctttctac acagcttgat gtcaaagtat acaaagaagg aaatgtgtac
    22621 tatcaagaat atcgtcgtgg agctgtagtg gacgacttaa agattatcga acaaacggat
    22681 cgccatggaa caacggttca ctttacgcca gaccctgaaa tttttacaga gacaactgag
    22741 tttgattttt caaaattggc tacccggatc cgtgaacttg ctttcttgaa ccgcgggatg
    22801 cgtatatcga ttgaagacaa acgagaagaa gaaccagtca tcaatgaata ccattatgac
    22861 ggcgggatta aaagttatgt cgaatacttg aatgcaaaca aaaccgtgat ttttccagaa
    22921 cctgtatatt tggaaggtga acagcaagat atcgcagttg aagtctctat gcaatataca
    22981 gacggctacc attccaatat catgagtttt gccaacaata tccatacgta tgaaggcgga
    23041 acacatgaat caggattcaa gactgcttta actcgtgtga taaatgatta tgcccgaaaa
    23101 caaaagctga tgaaagaaaa tgacgacaac ttgaccggtg aagacgtgcg agaaggattg
    23161 acagctgtta tttctatcaa gcatcctgac cctcaattcg aaggacaaac aaaaacaaaa
    23221 ttgggcaatt ccgaagtacg gacagtcact gatcggttat ttagcgaaca tttcatgaaa
    23281 ttcttgctgg aaaacccaag tgtgggccgt caaattgttg aaaaaggatt actagcttcc
    23341 agagctcgtc tagcagcgaa acgtgcgcgt gaagtaacga gacgtaaagg tgctttagag
    23401 attagtaatc tgccaggaaa attagcggac tgctctagca acgatcctga aaaatgcgaa
    23461 ttgttcatcg tcgaaggaga ttcggccggc ggatcagcta agcaaggtcg taatcgagag
    23521 tttcaagcca tcttgcctat tcgtgggaaa atcttgaatg ttgaaaaagc aagcatggac
    23581 aagatcttgg caaatgaaga gatccggtct ttatttactg ctatgggtac agggtttgga
    23641 gaagattttg atgtatcgaa agctcgctac cacaaattag ttatcatgac tgatgccgat
    23701 gtcgacggcg cgcatatccg tacattgctg ctgactcttt tttatcgtta catgcgtcct
    23761 attgttgaag ctggatacgt ctatattgct cagccacctt tatacggggt aaaacaaggg
    23821 aagaatatta cgtatgtaca accaggaaaa aatgcagaag aagagttgaa acaagtcgtt
    23881 gcttcacttc ctgcctcacc aaaaccaagc gttcagcgtt ataaaggact tggagaaatg
    23941 gatgatcatc aactatggga aacaactatg gacccaagca atcggatgat ggcccgcgtc
    24001 agtgtggacg atgctattgc agcagatcag atatttgaaa tgttgatggg ggatcgagta
    24061 gaaccccgtc gtgcattcat cgaggaaaat gcacactatg tgaagaactt agatatttaa
    24121 tcatgaagga ggaaaaagaa tgagtgaaga aatcagagaa aacatccatg atgtcaatct
    24181 aaccagcgaa atgaaagact ccttcatcga ttatgcgatg agtgttattg tcgcacgtgc
    24241 acttcctgat gtcagagatg gtctgaaacc cgttcatcgc cgtattttat acggcatgaa
    24301 cgaattaggt gtaacaccgg ataaagccca taaaaaatct gccagaatcg tcggagatgt
    24361 catgggtaaa taccatcctc atggagacta tgcgatctat gagtcaatgg tacggatggc
    24421 acagccattc agctaccgtt atatgctagt ggatggtcat ggaaactttg gttctgttga
    24481 cggtgacggt gccgccgcta tgcgttatac cgaagcacgg atgagcaaaa tcgctacaga
    24541 aatgctgcgt gatatcaata agaacactgt tgatttccaa agcaactatg atgatacaga
    24601 aaaagaacca gttgtactac ctgctcgctt cccgaatctt ttagtgaacg gaacaacggg
    24661 gatcgccgta ggtatggcca cgaatatccc acctcataac ttgtctgagg tagtagcggc
    24721 tatcgatttg ctgatggata atcctgatgt gacgaccaat gaattgatgg aagtattacc
    24781 tggaccagat tttccaacag gtggtttggt tatgggaaaa tcaggaatcc gaagagcgta
    24841 tgaaacaggt aagggttcta ttaccgtacg tgcaaaagtt gagattactg agatgccaaa
    24901 tggtaaagaa cggatcatcg tgacagaatt gccttatatg gtcaataaag caaaattgat
    24961 cgaacggatt tctgaactgc acagagacaa gcgtatcgaa gggatcacag atttgcggga
    25021 cgaatcttcc cgtgaaggta tgcggatcgt cattgacatc cgtcgtgacg ccagcgcatc
    25081 tgttatcttg aataacttat ataaattgac atccttgcaa aattcatttg gattcaatat
    25141 gttggcgatc gaaaaaggtg tgccgaaagt tttgagcttg aaacaaattc ttgaaaacta
    25201 tatcgaacat cagcgcgaag tcatcacaag acggacagtc tttgaaaaag aaaaagcaga
    25261 agcaagggca cacattttag aaggtttgcg tattgctttg gatcatatcg atgaaatcat
    25321 tgcgattatc cgcgggtcta aatcagatga cgaagcaaaa gcgacgatga tcgaacggtt
    25381 cgatttatcc gatcgccagt ctcaagctat tttagatatg cgtttacgcc gattgaccgg
    25441 tttggaacgg gaaaaaatcg aaaatgaata ccaagaactc ttaaaactga ttgaagattt
    25501 aactgacatt ctggcaagac cagaacgtgt tacagaaatc atcaagaatg aattagcaga
    25561 cttgaatcaa cgatttggtg ataaacgacg cacagaatta ctagtcggtg aggtcttaag
    25621 tctggaagat gaagatttaa tcgaagaaga agaaatcgtt attacattga caaacaatgg
    25681 ttacatcaaa cgtttggcaa ataatgaatt cagagctcaa cgaaggggtg gacgtggtgt
    25741 ccaaggaatg ggtatccaca atgacgactt cgtcaaaaat ctggtttctt gttctaccca
    25801 tgatacattg ttattcttta cgaataacgg aaaagtgtat cgggcaaaag gatacgaaat
    25861 tccagaatac gggcgcacag ccaaaggaat accaattatc aatttactag gtatcgattc
    25921 gagtgaaagc atccaggcaa tcattgcggt gacgggagaa gcagaagaag gccactatct
    25981 cttctttacg acgcgtcaag gtacagtaaa acgtacttct gtcactgctt ttgctaacat
    26041 ccgaagtaat ggtctgatcg caattggttt gaaagaagac gatgagctcg tcaatgtgct
    26101 actgacagat ggtcaatcta atatcattat tggaacacat aacggctact ctgtcacttt
    26161 caaagaagaa gcagtccgcg atatgggacg tacagcttcc ggggttagag gaatacgcct
    26221 gcgtgaggaa gattatgtcg ttggggcagc cttaatgaag gaagatcaag aagtattagt
    26281 gatcacagaa aaaggttacg gaaaacggac aaaagtatcg gaatatccag tgaaaggccg
    26341 cggaggtaaa gggatcaaaa cggcgaacat cactgaaaaa aatggtccac tagctggatt
    26401 gaccactgtt tctggagatg aagacatcat gctgattaca gataaaggtg tcattatccg
    26461 tttcaacgta tcgactgttt ctcaaactgg acgttcaaca ttaggcgtac gtttgatgaa
    26521 gatggaagct gatacgaaag ttgtcacgat ggcagcagtc gaaccagaag ctgcagaaca
    26581 aacagttgaa gaacaaggat cagaagaata gccatcaaga ctaattgtgt aagctagcaa
    26641 gaatgaccga ttaaaaaatc tggttattcc tgctggcttt tttgattagt tgaatttctc
    26701 ttaaaatatt cgaaactgtt cattctgtat tgcttttgac caaaatagag tgtataatca
    26761 gagcttgtga gtagtctaat taattagata actctccttg ctcttaggaa cctaagagcc
    26821 gaaagtccac agggaggtga atatcaatgg aaaacacgaa atatgaaatc atgtacatta
    26881 ttcgtccaaa cattgatgaa gaagcaaaaa ctgctttaat cgaacgtttc gatgcaatct
    26941 tgaaagataa cggagcagaa gttatcgaat caaaagattg ggaaaaacgc cgtttcgctt
    27001 atgaaatgaa cggttaccgt gaaggtatct atcacatcgt taaagtatct tctccatcat
    27061 cagcaaatgc tgtcaatgaa tttgaccgtc ttgctaaaat caacgacgat atcattcgtc
    27121 acatgattgt taaagaagaa gcttaattgt tgtttcatgt gaaacaatag aaaacgatga
    27181 gaggagatga ctattttgat taataatgtt gtattagttg gacgtctgac gaaagatcca
    27241 gatttgcgtt acacagcaag tggtacggca gtcgcaacat ttactttggc tgtgaatcgt
    27301 aattttacaa accaaagcgg caatcgcgag gctgatttta tcaactgtgt gatttggcgc
    27361 aaatcagcag aaactttagc aaactatgct cgtaaaggaa cacttttagg tgttactgga
    27421 cgtatccaaa ctcgttctta tgataatcag caaggacaac gtgtatacgt gacagaagta
    27481 gtagctgaca acttccaatt gctggaaagt cgttctgcat ctgaaaatcg ccaacaaagc
    27541 ggcggattcc aaagttctgg tcaatcagct ggcggatttg gcggaaacaa taactctaat
    27601 caaacatctc aatcatccaa cggtatgcct gatttcgacc gtgatacttc tgacccattc
    27661 ggctcaagtt caacgatcga catttcagac gatgatttac cattctaaac gaatttgata
    27721 ggagggaaat agaatggcac aacaaagaag aggcggacgt aaacgtcgta aagtagacta
    27781 cattgctgct aaccatattg aatatatcga ttataaagat atcgaattac taaaaagatt
    27841 tatttcagaa cgcggtaaaa tcttgccacg tcgtgtaact ggtacaggcg ctaaaaacca
    27901 acgtaaaatt actgttgcaa tcaaacgcgc tcgtatcatg ggcttgctac ctttcgttag
    27961 cgaagactaa taaatcctag gaattgaatg actgatcctt ttgatcagct attcaattcc
    28021 tttttttctg taaaaataac ctctattcgt ttagtcgctc gaatgacgaa agttatggta
    28081 aaatcaaatt agtcatatat gggaggaaaa ttatggaaaa taaaaacaat catttatccc
    28141 cgaaacctct cttccttatc ctgtttatat taatcgaagt attggtagtc ttaattttac
    28201 catttcgttt tgttagcgct tcggtcattc tattgatgaa tttatggggt gttcgctatt
    28261 ggttgaaaat gaaacgttgg cttattttaa ataaacgtga aaaaatccag caggcttcga
    28321 ctgtagctga aaccaatcta gcttatgtct cagaagtcat gcctgtagga gtgattgctt
    28381 atcagccatc aagccaaaaa atcgaatggc tcaatccatt agcattgaaa atcaaagatc
    28441 aaagtggatt aacagaggaa gaattgctag atactttttt tactgcaaaa gaaaaaaaga
    28501 aaaaccaagt ctatctagca gataaaacat ttcagtttga tatggataca gtaaagggtg
    28561 ttgtttattt taatgatatt acccaaaacc agttgttaaa actccatcag acgaacagtc
    28621 agccggtgat tggtatttta tctattgata actatgcaga tgccattgat aaattggatg
    28681 acaaagagat ttcttatttg aactctttcg tcactacact agtttctgac tggatgaatg
    28741 aataccatgt ctttttcaaa agaatcaacg cagaacggtt tttcttcgta gcacagatga
    28801 tcgatctgaa aaagatgatg gataaaaaat ttgatttgct ggatcgcttt cgagcagaag
    28861 ctgaaaaaca gagctcgcct atcaccctta gtatggggat tgcttatggc tcaagaaccc
    28921 tcgatgaaat cggtggagaa gctcagaata atttagatgt tgctttagtc cgtggaggcg
    28981 accaagttgt cgtgaaagag gtaaaagaag gagcaaaacc tgtttattat ggggggaaat
    29041 ctgctaagaa tgtcaaacgc acgcgtgttc gttctcgagc gatgagtacc gctttgcgtc
    29101 ggatttttga tgaaacaggc gatgtgttta tcatggggca caagtttccg gatatggatg
    29161 ctttaggctc tgcttttggt gtcagttgtc tggcacgctt caaccagaaa aaagcttaca
    29221 tcgttatcaa cgagaacgag atcattccag atgtagaacg gtgtcttgaa gaaatccata
    29281 agcatcctga attagaaatg caactaatct ctccagaacg tgccatagag ctgaaaaaag
    29341 aagaggacct cctcgtgatg gtggattacc ataaaccttc tctatcgatc tctcagggcc
    29401 tatatgagca atttgataag attgtgatca ttgatcacca tcgtcgtggg gaagagtttc
    29461 caagtaaacc tctgctgact tatatcgaat cttcagcttc ttcagcttca gagctagttt
    29521 ctgagctgat ccaataccaa agcagcaaaa caaatcgctt agatcgtttt gaggccacat
    29581 tattgcttgc agggatggtt gtagatacaa atagtttcaa tgtccgtaca actgcacgaa
    29641 cttttgacgt tgccagctat ttaaggtctt gtggagcaga cgcatcctta gtacaatatt
    29701 tattgagctc ggatttagcg acatacttgg agatcagcaa tttgattgct ggaagcgact
    29761 atgtgacttc tgatattgtt gtggcggcag ggacagacga aaaagagtat aatagtgtta
    29821 tagcagcaaa aacagcagat acattgcttt cgatgatcaa tatcaatgca gctttcgtca
    29881 ttacaaagcg gacagacggc ttgattggga tcagtgccag aagtaccggc acgatcaacg
    29941 tccaaatcat catggaatcc ttaggtggcg gaggacactt cacaaacgca gctactcaac
    30001 tggaaaatac gacagttgag gaaacaaaac agaagctatt tgctgcaatc aaagaaaata
    30061 tgaatcaaat atatgataag gagtaatcca aatgaaagta atttttttac aagatgtaaa
    30121 aggaaaaggt aaaaaagggg acatcaaaga agttccagca ggttatgcac aaaattatct
    30181 aatcaaaaat aacttggcaa aagaagcaac gaaaagcagt atcagtcagt tagcaggaca
    30241 aaagaaagca caagaaaaac acgaagctga aatcttggaa gaagctaaaa agttaaaaga
    30301 attcttggaa aaagaagata ctgtagtcga attgaaggcg aaagcagggg aagacagccg
    30361 tttgtttggt tcgatccctt caaaacaaat tgctgaaggg ttaaacaaac aatacaaaat
    30421 caaattagat aaacgaaaaa tcgaattaga aaatccgatc cgttcactag gctacacaaa
    30481 cgtacctgtt aagcttcatc atgaggtgac ggctacgatc aaagttcatg tcgtacaaga
    30541 ataacaaatc ttgatagcga tgttcagctg gtaagtataa ccagaaggaa aaaagacaaa
    30601 ccgaaattta tctgagaaaa cagctgcagc aatgaaagca aaagctgcaa atcgatttca
    30661 gattgatttc ggtttctttt ttgggaacag caaaatagca taaagaaaac gagtataata
    30721 aaagagaact atttttttat tacactttcg gtacgaatga tgacttttct ctcattttct
    30781 tgtaaaatga agcaattgaa gacaaggagc gatacgtatg aatgaagtgt ggcaggatcg
    30841 aatcccaccg caaaatattg aagctgaaca agcggtttta ggctctgttt ttcttgacgc
    30901 agaaacgatc attgatgcca tggagtacat agagccaaaa gacttttatc gtcgtggcca
    30961 tcaaattatt tttgagacaa tgatcgagtt aaatgatcgt aatgaagcga ttgacgtggt
    31021 tactgtcaaa gaccggctgg aacaagccaa tttgttagaa gatgccggtg gtttaagtta
    31081 tctctcagac ttggcattag cagttccgac agcagcaaat atcgtctact atgcgaaaat
    31141 cgttgaagaa aaatcattgc tgcgtacatt gatccagaca gcaacaggaa tcgttacgaa
    31201 aggattcgag caaggagaag aagttcaaac aattttagat gatgccgaac gcagtatctt
    31261 ggaggtttcg gaaaaaagaa atcgaagcgg ctttttatct attgctgaag tactgaactc
    31321 cacgattgaa aatatcgatc gactagctca aaacaatgaa gaaatcacag gtctaccaac
    31381 tggctatcaa ggtctggata aaatgacagc aggtttccag cccgaagaac taatcattct
    31441 tgctgcacgt ccagcggtag gtaaaacagc gtttgcattg aatattgctc aaaatgtagg
    31501 aacaaaaaca gatcgttctg ttgcgatatt tagtttggaa atgggggcag aatctcttgt
    31561 aaatcggatg ctttgtgcag aaggttcgat tgaggccagc catttacgta caggtcagct
    31621 ttcggaagaa gaatggtcga atctgatcgt tgcgatgggg agtctttctc gagcaaatat
    31681 ttttatcgat gatacaccag ggatcaaaat ctctgaaatc cgtgcgaaat gtcggaaact
    31741 agcgcaagaa aaaggcaatc taggcttgat attgatcgat tatttgcagt tgattgaagg
    31801 taacggtaaa gagaaccgtc aacaagaagt atcagatatc tctcgccaac tgaaaaaact
    31861 ggcgaaagag ctgaaagtac ctgtcattgc tctttcacag ttatctcgtg gagtagaaca
    31921 gcgacaagac aaacgaccag tgctaagtga tattcgtgaa tccggatcaa ttgaacaaga
    31981 tgcggacatc gtagccttct tgtatcgaga tgattattat gaaagagaag gcggagatga
    32041 cgatgatgga aacgagccaa aaaatgacaa cgtcatcgaa gtcatcatcg agaagaaccg
    32101 aagcggtgca cgaggaacag ttgaactact gtttatcaaa gaatacaata agttttcgtc
    32161 gctttcgcca agagaagaat tttaaagaag aggttgtgag ccttgagcaa tcccgagagc
    32221 tctcgtaaga agatgcaagg agtaaccgca ggaacgacta gagtacttaa tttaaacaga
    32281 tctccaaagg gattgtgaca aaagtcttgt cacagtccct ttttccgaat aaacggtgtt
    32341 caatcagctg acccttggta ttagctcagc aaacgtcaaa atatgaggaa ctattttgac
    32401 gtttgtttct ctaatactca ggtcaaaacg ctgatcttac gacctctttt ccttaaacgg
    32461 tgttctcctg ccaactcctc ggtcacaagt cacaatatca tcaaacatga gaagcagttt
    32521 tttgaaattg ctccttgtga ctcagagtta aacggcaggc tcacaacctc tttcttaaac
    32581 ggtgttcaaa agcttgcatc tgcggtaata cgcccaatca aacgaaaaat atgagaagct
    32641 atttttgttt ggctgttctt attacttgat gcaggacagc tttttcacaa cctctgattc
    32701 acggtgttcc aaaagtagct tcttcgaaaa ataagtcgaa atttcacaaa aatttgagaa
    32761 gcaattttcg tacattcctt cttatttctt gaagctgacc acttctgtta caacctcttt
    32821 tttaataacc attctaaaaa gataaagttc gttttttaag cgtttttata aaataaaaat
    32881 aaaaatcatt cgattttatt tgaaatatgt atctattatt gatacaatgg ttcaggatta
    32941 aataatatag aacgaggtgt tcacatgtca tctgtagtag tagtaggtac gcaatggggc
    33001 gatgaaggga aaggaaaaat cacagacttt ctcagtgaaa atgctgaagt gattgcacgg
    33061 taccaaggtg gcgacaatgc agggcacaca attaagttta acggtgtgac ttataaactg
    33121 catttgattc cttctgggat tttttataaa gacaagatca gtgtgatcgg aaacggcgtt
    33181 gtagtcaatc ctaaatcatt ggttaaagaa ttagcttatt tacatgaaaa aggtgtagaa
    33241 acaagtaatt tgcgtatttc tgaccgcgcg cacgtaatct tgccttatca tatcaaattg
    33301 gaccaattac aagaagatgc taagggcgaa aataagattg gtacaacgat taaaggaatc
    33361 ggacctgcct atatggataa agcagctcgt gtaggtatcc gtattgcaga cttattagat
    33421 aaagaaattt ttgaagaacg tcttcgtttg aatctagatg acaaaaaccg tcaatttgta
    33481 aaaatgtttg attcagaacc acttgatttt gacgatattt ttgaagaata ttatgaatac
    33541 ggacaacaaa tcaaacaata cgtcacagat acttccgtta tcttgaatga tgctttggat
    33601 gcaggcaaac gcgtgttgtt tgaaggcgca caaggtgtaa tgctggatat cgaccaggga
    33661 acttatcctt ttgttacttc ttctaaccca gtagccggtg gggtaacaat cggaagcggt
    33721 gtagggcctt caaaaatcaa taaagttgtc ggcgtctgca aagcctatac gtcacgtgtc
    33781 ggtgatggac catttccaac tgaattattt gatgaaacag gagaacaaat ccgcgaagta
    33841 ggacgagaat acggtacgac aactggtcgt ccgcgtcgtg taggctggtt tgattctgta
    33901 gttatgcgcc attctaaacg agtttcaggt attaccaacc tatctttaaa ctcgatcgat
    33961 gtattaagtg gattggaaac agtaaaaatt tgtacggctt atgaattaga tggcgaacta
    34021 atctaccact atccagcaag cttgaaagag ttgaaccgct gcaaaccaat ctatgaagaa
    34081 ttgccaggct ggtcagaaga cattacagga tgcaaaacac ttgcagaact gccagaaaac
    34141 gcacgtaact atgtacgtcg tatctcagag cttgttggtg tacgtatctc aacattttct
    34201 gtcggacctg atagaacaca aacaaacatc ttagaaagcg tctgggcgca aatctaagga
    34261 taaaaacgac gactgaatca ggacgttctt accagagcga gtcgaagtgt ttatctatac
    34321 gataataata acttgaacac tgtttattca gaataaggga ctgtgacaaa aaccgtgtca
    34381 cagtcccttt ttccgaacaa acggtgttca aaagcttgca tctacggcac taagcccaat
    34441 cagatgaaaa atatggagtg ctatttttat ctgattgttc ttactgcttg atgcagagca
    34501 gctttttcac aacctctcca ctaaacggtg ttctcctgcc aactcctcgg tcacaagtca
    34561 caatattcaa caaacatgag aagctgtttt ttgaaattgt tccttgtgac tcggagctaa
    34621 acggcaggct cacaacctct tggtaaacgg tgttcaatca gctgaccctc ggtattagct
    34681 caaaaaacgg caaaatatga ggaactattt tttccgtttt tttctctaat actcaggtca
    34741 aaacgctgat ttcacaacct ctgattcacg gtgttccaga agtagcttct acggaaataa
    34801 gccgaaattt cacaaaaatt tgagaagcaa ttttcgtaaa ttccttctta tttcttgaag
    34861 ctgaccactt ctgtcacaac ctcttatttt gattagaact tgcgaaaata aaccggttaa
    34921 agtgtgtcta gcgcaagaga tttgctataa tagacgataa gggtaaaatg taacgagaag
    34981 gggttgaaaa atgtgtatca aattgtaaca gattcttgtt gtgatctgcc ataccatgta
    35041 ttagcaaaag aaaaagttga ttttttatca atgaatctcc aattgaatga tcaagagtta
    35101 gtagatgacc tcggaaagac attcaattat gaccgctaca tgaatcagct gaaaaatggt
    35161 gcgatgccta cgacatcaca aatcaatgtt ggccgatatt tagaattttt ccgttcttat
    35221 gcggaaaaac agattcctgt tttatatgtg gctttttcca gtggattaag cggctcttac
    35281 agtagtgcgc ttcaagcggt tgaattgctg aaggaagaat atgaagaccc tgagatctat
    35341 gtttttgata caaaagctgc aagtattggg gaagggcttt tagtattgga agctgtaaga
    35401 ttgaaaaaag aaggtaaaag catcgaagaa accctagagt ggctggaaag taattatttg
    35461 aaagtccatt catgggtaac agtagacgat ctgaaatatc tagaacgagg cggtaggatt
    35521 tccaaagcaa gtgctactct tggcagtcta ttgagtgtaa agcctattat tacggtggat
    35581 aaagaaggaa ggctgacgaa tgttgacaaa gttcgaggaa gaaacaagtc gatccagaca
    35641 atcgtcaatg agacggtaaa aacaatcgtt gcaccacttg atcaaatcgt ttatgtggct
    35701 tacgctgggg atctagagag cgcggagaag gcaaaacggc ttttggaaga gcagataaag
    35761 atgaaagatg tgaagcttta tcccttagga ccaacgatcg ccagtcacac aggctatggc
    35821 tgtatcgcga ttttttcgat gggaatcagt cgataaaagc agtaactcat tctgtaaaaa
    35881 agcccagaaa ttgattgtaa caaaaacagc gactcttaaa agatagatga gcatcctatc
    35941 tcttagaggt cgctgttttt ttatttttta agctaaatag gccattatgc ccataactcg
    36001 ttcaattgtt tttgaatatc tgcattttcc aaaaattcat catatgtcgt atcagctcga
    36061 tcgatcacac ctttatccga aacggcaatg atgcggtttg ccaatgtttg gataaattga
    36121 tggtcatgtg acgcaaataa gattgagcca gtaaatgcca tcaagccgtc attcaatgct
    36181 gtgattgatt ctaagtctaa atggttcgtt ggatcatcta atacaagtac gttagctttc
    36241 gaaagcatca tttttgaaag catgcaacgg actttctctc caccagataa aacattcact
    36301 ggtttcaata cttcttcacc agaaaacaac attcttccta agaagcttct taagaaggtg
    36361 ttatcatctt cttctttact tgcatattga cgcaaccagt ctaaaatcgt tagatcagta
    36421 tcgaattctt tcgttgtatc ctttggtaaa taagaattac ttgttgtcac accccaacgt
    36481 actgttccag tatctggcgt aatttcaccc atgataactt tgaatagcgt tgttgttgtt
    36541 atatcatttt ctgcgacgaa agcgaccttg tcttctttgc tcaaattaaa agatatatca
    36601 tctaaaattt tctttccatc aatcgttaca cttacatttt ctacctgcaa taagtcgttt
    36661 ccaatctcac gttcaggttt gaatccaacg aatggataac gtctagaaga aggttggata
    36721 tcgtctaaag taattttttc aagcattttt ttacgtgaag ttgcttgttt tgattttgat
    36781 gcattcgcac taaagcgggc aataaattct tggagttctt tgatttgttc ttcttttttg
    36841 gcatttgcgt ttgcttgaag tttagccgct agttgactag attccaacca aaaatcataa
    36901 tttcctacat aaagtttgat tttaccaaaa tctagatccg ccatatgtgt acatactttg
    36961 ttcaaaaagt gacggtcatg ggatacaacg atcacagtat tttcaaaatt gatcaaaaat
    37021 tcttccagcc aattgatcga ttggatatcc agaccgtttg ttggttcgtc tagcaaaaga
    37081 acatcaggtt taccaaatag tgtttgtgct aataacactt ttaccttttg tccaccagtt
    37141 aattcactca tttgctgatg gtgcaattct tcagggatgt tcagaccttg cagtaaaaca
    37201 gctgcttctg gttcagcttc ccatccatta agctcagcaa attctccttc taattcagct
    37261 gctcgaatac catcttcgtc agtgaaatcc tctttcatgt aaatcgcatc tttttctttc
    37321 agcacttcat acagacgctt gtgtcccatg atgactgttt caagtacagt atagtcttca
    37381 tagtcgtaat ggttctgttt caatacggcc atccgttcat ctggtcccat agtaacggaa
    37441 cctgtggaag gctgcagatc ccctgataac actttaagga acgttgattt acctgcacca
    37501 tttgcaccaa tcaagccata gcagtttccg ggggtaaatt taatattcac atcatcaaat
    37561 agtttgcggt ctgaaaagtg caaactcaca tcatttacag taatcaattt ttttcctcac
    37621 ttttatcatt attagttttg acaagtctta tagaattata taggggaaag gtaaaagatt
    37681 caaggcaatc gaacattctt tgtgtttcac gtgaaacaga atgaaaaaaa taaaaataaa
    37741 agcggagcga aaatagctac tataggattt attagtttgc aacagcataa tttttgacgt
    37801 tatttataag agggagtact ataaaactgt aaaagaaatg aaagaaggat tgaaaatgga
    37861 tgaaaaaaga accatcgact ggggctcatt gattttggga attttatttg tacttgtctc
    37921 attgttatca tttcaagacc ctgtcggaaa tttagttgca attgtagtag tctttgcgat
    37981 ttttgccttt attaaaggga tatttgaact gtttgtacgg aacagattaa aagagctaac
    38041 aggatataaa ggaaagatgc ccttaataat cgggatcatc gatattctag ttggcgtctt
    38101 tttcttattc aatattggtg caggagtcgc agcattgcct tttgtttttg cagtatggtt
    38161 tattgctgat tcagttttag cgttatttac tgctgacttg gcaagaggag taagtgaagg
    38221 atattattgg tttacgatca tcgtcaatat tttaggaatt ttattaggaa ttttcttatt
    38281 gtttaatcca atttcttctg cgttgacatt aagcttttta gttggattct attttatgtt
    38341 atttggtatc acacatatcg tctatgcatt tagataagta aaatcaaaca aataaaaaag
    38401 ccggcttccg attacagttt tactgttgta attggaagcc ggctttttta tcatggacaa
    38461 atatgcacga ggtgattttt aggtgtcgag acaaatttat gcaaaaatgt gagaagctat
    38521 atacacaaat tttatcttaa tactcgaagc atagcgcagt tgtcacaacc tcttttcata
    38581 taattcattc aatactggca aatcatggac ttcatatttt tctttgaatg cttggatttc
    38641 ttcttcagaa tattttcttg gatctaaatg cagatcttct actggcaatg ggcgcttgtt
    38701 attgatcttc acatcgatta ctacaggacg atctgatttt gcagctgcat caaaggcaaa
    38761 tcgaagttgc gagtattctg tgactgtaaa gctatttgcc ccgagcgctt ctccaacttt
    38821 accgaaatct gcatcttcta aaaatacacc aaatttcttt tgttccgtat cttcttgttc
    38881 tgcttcgatg aaaccaaaag aatcattcga aaagacaaca ttaataattg gaagctgata
    38941 tttgacttgt gtaatgatat cttgcatgac catagcaaac ccgccatcac cacttaaggt
    39001 aaatacttgt ttttctgggt agcttagttg tgcggcaatt cctcctggca caccattacc
    39061 catagttgcg aaccatccag atgtcgtatg tttttgttta ccgttcatat tcaataaacg
    39121 aatagaatgg atagtcgtgt taccgacatc tgtgacgaaa atcgcatcgt cttcagcaat
    39181 acgattgatt tctttataga cagcctctgg acgaatcggt tcttcctctc gatcttcaaa
    39241 agatttcaac caatttaccc atttttcttt attttcttga tttgctaggt accagctatc
    39301 agcagggcga gcatccccta actcgacaag tctttgcaaa gctgtgtttg catcaccgag
    39361 gatcgacaaa gatgtttcat ggcgtcgtcc aaattttgac gcatcgatat cgatttggat
    39421 aaattcagca gttggattaa aaaatgcacg tccaaatggg aagtcgctac cgacaaataa
    39481 gatcagatcg gcttctgcca atgcttcatt tccgggttta gtagcgacac gtccagcaaa
    39541 accaagaaag ttttcataat catcaggaat aattcctttt gctagaacgg aagcgacaac
    39601 aggaactgaa aaatgttctg agaattcttt gatttgcgga aatccgtttc gcgttccttg
    39661 accaacataa agaactggtt tttttgcttt ttcaagataa ggcaaagcag ctagcaaatc
    39721 tttttcttct ggtaatatca cacttgtttt gtgagtgtga gcggtggaga aaggttgttc
    39781 gtcaatttct tcaaaaccaa gatcaacagg gatagtcacg atagccacgc ctttatgctc
    39841 ataggcggct ttaatggctt catctactac atgcggaaga ctttgtggtg tcatgactgt
    39901 acgattgtat acgctaacat ctgcgaaaat aggattttca tttagttcct ggaaagagtt
    39961 atagttcatg gatgtagaag caacttgtcc taagaacgct actagaggca catggtccat
    40021 ctgtgcatca tacaaaccat tgatcaaatg tgtcgcacca ggtcctgcag agccgaagac
    40081 agcgccgact ttccccgtta attttgcatc tgcagcggct gcgagtgcgc cgacttcttc
    40141 atgacgaact tgaatgtagt tgatttcggt tttttcttga tatagcgcat ccataattga
    40201 attgaaagat cctccgggaa tgccgtaaat atggtcgata ccccagcttt caagaacttt
    40261 taccattgcg attcctgcat tgatttttga catggtgttt cactccttta gcaatgatga
    40321 tacataaatt ttttgtatct agagtataga actttttatc gaaaatccca actaattcgt
    40381 cttttcacaa aaatgtagtc atgtttttgg gaatatgcta gagtagtaca tagatagatt
    40441 caagagaggg gaatggcatg gttgaatggc tattgagact tgaaccttgg cagcaagcac
    40501 ttgctgggac agcttttact tattttatga cagctcttgg tgccgggttg gtttttttct
    40561 ttaaagaaat caaaaaagaa gtgctaaact tgatgttagg atttgcttct ggtgtcatga
    40621 ttgctgcaag tttttggtct ttgttagatc cggctattac aaaagcagaa gaaaatggag
    40681 atattgcttg gctagttgta agtattgggt ttggtttagg cggtgtgttt ttatatatgg
    40741 cagataaaac actgccccat atgcattttg gaccacaaca tgaagcagaa ggattgccta
    40801 cccatttaaa acggacgatc ttgcttgttt tttctattac attgcataac attcctgaag
    40861 gtttagccgt gggggtagca ttcggtgcag cagcaactgc ggataatccg acagccgcta
    40921 tattagccgc tgtttcggtg gcgctaggta ttggtatcca aaacttccca gaaggagcag
    40981 ctgtatcaat cccgttgcga caagaaggtc tgagtcgtaa aaaggccttt gtttacggac
    41041 aagcttctgg aatcgttgaa ccaattgctg gagtgattgg agcgctacta gtaacgaaag
    41101 tggagctgct gcttccttat gcacttgctt ttgccgcagg agcaatgatt tatgtcgtgg
    41161 tggaagaatt gatccctgaa gcacagcaga cattgtccag caaacgtcat tttgcagttt
    41221 ttggcgtcat gagtggattt atcattatga tgattttgga tgtggcatta ggataaataa
    41281 aaaaaatata tatgatatat caaatagggc cgcgaactta tatcgcgacc ctattttttt
    41341 agtaagttat atcataatga agtgaatggt atcatacatt aataatagtt ttttctatgt
    41401 gtatttcctt tgatccagcg aacaagccag atgataagtg cgattgggat tagaactttg
    41461 accgcaaacc ataaaagccc gcttaggaca ctgacaaaaa gactgatgac gaagctagct
    41521 gcaattagca ctaaaagaac catgaagata tttaagaaca tatttttttg acgcatacaa
    41581 aaccctcctc ttgttcattc tttactatag aaaaagtata acagggattg tttaaaaaca
    41641 cctccaactg aagactgatt tttacttcat ctacttaaat gatcaaacaa ttgctttttc
    41701 tctggtttat catctaaaat agagcggaaa aggaggagaa tatcatgcct aaaaatcgtc
    41761 ttgaagcatt tacagatgct gtaatcgcga ttatcatgac tattttagtt ttagaacttc
    41821 atgaacctaa cggagacacg tgggaggcgc tcgctgcatt aggttaccga ttttttatct
    41881 atattgtcag ttttatcatt ttagcgattt attggaacaa tcatcatcat ttatttcaag
    41941 ttgtacataa gataaatgga cgggtcttat gggcgaacag tttctttgtt tttgcgcttt
    42001 cgctttttcc gtttgcaact tcatggatca gtgagtacat caatagcctg gctccagaaa
    42061 tgacttatgg ttttgttacc ttgaccgcta atattgcgta ttatatcttg gcaaaagaac
    42121 ttgtcaaagc aaataaaggc cacgagaaag cattgaaggc gatgttgaat aattacaaga
    42181 aatcgtatct atcgattggt ttgaatgtgt taggaattgt cttagggtat ttagtagcgc
    42241 cagtagccgt gctgatcgca aacatcgtga ttctagctgc ttggatcatc ccaagtcgta
    42301 ccatcgaatc acaatatcgg taaaatgatc cgtgtttaaa atttaagaga aaaatataga
    42361 aaaaataaat aaacgatcaa atatccgagt gatactagta ttcaaagagg gatgcagatg
    42421 gatcaaaaaa gaattcatgt aggactgatt actagttttg tcacgatgct tatttatttg
    42481 gatcaaggtc atttgacttg gacagtagtt ggtgtcgtag gatttttgac attattcaca
    42541 gcaataaaaa aataaacatc tttatgtaat ctgatgttct atctatttaa gaaaagcatg
    42601 tttacagcag tatgattctg ttgtaaacgt gtttttattt gtggaaaatt taatttttag
    42661 aaaagtgtat cgaaaaatag atagttagtt tttccacagt ggataacttc gtaagagata
    42721 aaaata
//
LOCUS       DAYYCY010000006        42374 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000006 DAYYCY010000000
VERSION     DAYYCY010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.006"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            567..1404
                     /locus_tag="ACHAZB_000259"
                     /pseudo
     CDS             567..1404
                     /locus_tag="ACHAZB_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
     gene            1469..1903
                     /locus_tag="ACHAZB_000260"
     CDS             1469..1903
                     /locus_tag="ACHAZB_000260"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8612973.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEE
                     AKKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(1971..3293)
                     /locus_tag="ACHAZB_000261"
     CDS             complement(1971..3293)
                     /locus_tag="ACHAZB_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HFM8612974.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFWDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(3447..5087)
                     /locus_tag="ACHAZB_000262"
     CDS             complement(3447..5087)
                     /locus_tag="ACHAZB_000262"
                     /EC_number="4.1.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043883 - malolactic enzyme activity
                     [Evidence IEA];
                     GO_process: GO:0043464 - malolactic fermentation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malolactic enzyme"
                     /protein_id="HFM8612975.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            5417..5887
                     /locus_tag="ACHAZB_000263"
     CDS             5417..5887
                     /locus_tag="ACHAZB_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HFM8612976.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            6516..7619
                     /locus_tag="ACHAZB_000264"
     CDS             6516..7619
                     /locus_tag="ACHAZB_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HFM8612977.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            7903..8583
                     /locus_tag="ACHAZB_000265"
     CDS             7903..8583
                     /locus_tag="ACHAZB_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFM8612978.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            8673..9707
                     /locus_tag="ACHAZB_000266"
     CDS             8673..9707
                     /locus_tag="ACHAZB_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFM8612979.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            9721..10056
                     /locus_tag="ACHAZB_000267"
     CDS             9721..10056
                     /locus_tag="ACHAZB_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8612980.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            10040..12520
                     /locus_tag="ACHAZB_000268"
     CDS             10040..12520
                     /locus_tag="ACHAZB_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8612981.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            12576..14135
                     /locus_tag="ACHAZB_000269"
     CDS             12576..14135
                     /locus_tag="ACHAZB_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8612982.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            14132..15496
                     /locus_tag="ACHAZB_000270"
     CDS             14132..15496
                     /locus_tag="ACHAZB_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFM8612983.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            15667..16737
                     /locus_tag="ACHAZB_000271"
     CDS             15667..16737
                     /locus_tag="ACHAZB_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8612984.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            16752..17420
                     /locus_tag="ACHAZB_000272"
     CDS             16752..17420
                     /locus_tag="ACHAZB_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8612985.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            17440..18018
                     /locus_tag="ACHAZB_000273"
     CDS             17440..18018
                     /locus_tag="ACHAZB_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8612986.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            18421..19332
                     /locus_tag="ACHAZB_000274"
     CDS             18421..19332
                     /locus_tag="ACHAZB_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFM8612987.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(19588..20505)
                     /gene="rnhC"
                     /locus_tag="ACHAZB_000275"
     CDS             complement(19588..20505)
                     /gene="rnhC"
                     /locus_tag="ACHAZB_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HFM8612988.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            20707..21144
                     /gene="zapA"
                     /locus_tag="ACHAZB_000276"
     CDS             20707..21144
                     /gene="zapA"
                     /locus_tag="ACHAZB_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFM8612989.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            21202..21749
                     /locus_tag="ACHAZB_000277"
                     /pseudo
     CDS             21202..21749
                     /locus_tag="ACHAZB_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
     gene            21864..24224
                     /locus_tag="ACHAZB_000278"
     CDS             21864..24224
                     /locus_tag="ACHAZB_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFM8612990.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            24311..24625
                     /gene="trxA"
                     /locus_tag="ACHAZB_000279"
     CDS             24311..24625
                     /gene="trxA"
                     /locus_tag="ACHAZB_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HFM8612991.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            24807..26585
                     /gene="uvrC"
                     /locus_tag="ACHAZB_000280"
     CDS             24807..26585
                     /gene="uvrC"
                     /locus_tag="ACHAZB_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFM8612992.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIERLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            26730..27629
                     /locus_tag="ACHAZB_000281"
     CDS             26730..27629
                     /locus_tag="ACHAZB_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8612993.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            27622..28884
                     /locus_tag="ACHAZB_000282"
     CDS             27622..28884
                     /locus_tag="ACHAZB_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8612994.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            29047..30945
                     /locus_tag="ACHAZB_000283"
     CDS             29047..30945
                     /locus_tag="ACHAZB_000283"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HFM8612995.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(31244..31963)
                     /locus_tag="ACHAZB_000284"
     CDS             complement(31244..31963)
                     /locus_tag="ACHAZB_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HFM8612996.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQRFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     misc_feature    32153..32200
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF03071"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="sodium ion sensor (DUF1646 type); this
                     cis-regulatory element may regulate processes involved in
                     with the transportation of sodium ions; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF03071"
     gene            32300..32632
                     /locus_tag="ACHAZB_000285"
     CDS             32300..32632
                     /locus_tag="ACHAZB_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8612997.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            32619..34622
                     /locus_tag="ACHAZB_000286"
     CDS             32619..34622
                     /locus_tag="ACHAZB_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HFM8612998.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            34641..35111
                     /locus_tag="ACHAZB_000287"
     CDS             34641..35111
                     /locus_tag="ACHAZB_000287"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HFM8612999.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            35132..35716
                     /locus_tag="ACHAZB_000288"
     CDS             35132..35716
                     /locus_tag="ACHAZB_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HFM8613000.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            35731..36717
                     /locus_tag="ACHAZB_000289"
     CDS             35731..36717
                     /locus_tag="ACHAZB_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HFM8613001.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            36707..37018
                     /locus_tag="ACHAZB_000290"
     CDS             36707..37018
                     /locus_tag="ACHAZB_000290"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HFM8613002.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            37040..38821
                     /locus_tag="ACHAZB_000291"
     CDS             37040..38821
                     /locus_tag="ACHAZB_000291"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HFM8613003.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            38814..40190
                     /locus_tag="ACHAZB_000292"
     CDS             38814..40190
                     /locus_tag="ACHAZB_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HFM8613004.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            40197..40832
                     /locus_tag="ACHAZB_000293"
     CDS             40197..40832
                     /locus_tag="ACHAZB_000293"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HFM8613005.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            40862..42217
                     /locus_tag="ACHAZB_000294"
     CDS             40862..42217
                     /locus_tag="ACHAZB_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFM8613006.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
BASE COUNT        13926 a         7366 c         8997 g        12085 t
ORIGIN      
        1 caaaagaatg aattaataag atataaaaaa agggattgtg acaaacgtct tgtcacaatc
       61 cctttttcca aataaacgat gttcaatcag ctgcccctcg gcattcgctc gaaaaacggc
      121 gaaatatgaa gaactatttt gccatttgtt tctctaataa tcaggtcaaa acgttgactt
      181 cacagtctct tccttaaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt
      241 caacaaacat gagaagctgt tttttgaaat tgttccttgt gactcggagc tgaacggcag
      301 gctcacgacc tctttcttaa acggtgttca aaagcttgca tctgcggtaa taagttcaac
      361 caaacgaaaa ttatgaggag ctatttttgt ttggctgttc ttattacttg atgcaggaca
      421 gctttttcac aacctctttt cgtttagcac taaatttcct taaaaagtag aaaagctttc
      481 gaagcctcgt ttgtttcttt tactgtataa taaaagaaaa gagtaacaat cgctaaaaga
      541 tataaacaag agaaaggaaa tacaagatga ttggtctgat ttattttgtg gtgatcgttc
      601 tggcgaacac agttggcgct atttccggga tgggtggagg cgtgttgatc aaaccgattc
      661 ttgatcttat cggcgcccat tctgttgcag ggatttcttt ttactcaaca gttgcggtat
      721 tcacgatgtc cattgtttcg actgtgcgac aagtatcaag cgggaaatca ctgaattggc
      781 agattgtcgg ttgggtatct agtggagctg ttgttggagg agtagtcggc aacattgtat
      841 tcgaagtttt tttacagtta tttgaaaacg agaagcatgt gcagctgatc caaatttttt
      901 tgacggtatt gaccttggtt tttgcgttct tctacaccaa gcatcatcag ccaaaatttc
      961 atttgacgtc atggacttgg taccttatat gtgggggggg ttctgggctt tttagctagt
     1021 tttttaggta ttggtggtgg accagtcaat gtttctttgt tgatgctgat gtttgcattg
     1081 ccaataaaag aagcaacact atattctcta tctacgattt tcttttctca actggcaaaa
     1141 ttggtaacga ttgctttaac aagtagcttt atgcgctttg atttatctat gttgttttat
     1201 gttatccccg ctgctgttat tggaggtttg tggggagcaa gatttagcag ggttttgtct
     1261 ccaaaaaaag tgacctttat ttttcaagta attgtcctcg tggttctttt ggtcaattgt
     1321 tacaatgcct ttgtcatctt acaaacatta ggagaatcta gactaacgca agtcaaaatc
     1381 actgaaacat attgttctaa atgaaaaaaa gagtatacta tgataaaaaa attcatttta
     1441 aaaaatcaga aattggctgg tgatttggat gttacgggaa atgcgtttag aagatgcaga
     1501 agcactacgt gaaatcaatg cggatcaatt agggtatgat ataccattaa ccgttacggt
     1561 gagacaaatg aagaaactgc tgaaggagcc agagaaaaat tttttcttgg tttatgaaga
     1621 tcctacttct aaaaaagcag ctggctatgt acaagcggag tcctatgaat cgatttttag
     1681 cgatccaatg ttcaatatta tggcacttgc tgtgtctaaa tatgctgaaa aaagaggcat
     1741 cggaaaggct ttgatgactg gcgtagaaga agaagcgaaa aaacgaggaa taacgacaat
     1801 aaggcttaat tctgcagaat acagagtaga agcacaccaa ttttatgaac acatcggtta
     1861 tcattccgat aaaatgcaaa aaagattttt gaaaatcctg taaatcttta tcaaaaaaat
     1921 acccgatcac caagccgata cttgatgatc aggtattttt gtttttttga ctaagaaagt
     1981 aattgcatca agaaaccaga gatgatcaaa acgatggcac caccaagtcg attgcccatt
     2041 tgagcaaaag caataagttc catccgatta gctgcagata agacggctac atttcctgtt
     2101 ccgcccatcg agttgttgca taagccagca gtaatagttg attcaattgg atataaacca
     2161 aacaaacgac cgacaaatcc tgaaacaact gaaatcgcga taacacttgt cagacataag
     2221 acaacaaatt gccatgtcaa agcagaagct aatacattca gattcagcaa cgcaataccg
     2281 ataccggcaa gaacagctgc tgtcaagttt ttgacaatca agttattgaa catgatagct
     2341 gcatcttcat aatactctgg taataggttg gcgattttac agatgacaac gataataatc
     2401 ataaatgcgt aagcatggac tttaggaaca aaatagttac caatttgtcc taagatgaag
     2461 aaagtcaatg ataccatcaa accaacacct aactgtggaa tggaaggttt cacagttcga
     2521 ggttttccat cgccaagatc gcctttttcc attaatttac cattgccgtt gaattttggc
     2581 attgattgtc cagcacgagc aactaatgct gcaccgatga tagctaagac atttgtcatt
     2641 gctgtcgcag gaatcaggcg ggaaatgata gaagaagcat ccgtacctaa aacatttgca
     2701 taaatcgttg ataaaggaac ggctccggct ccaacaccac cagccatcgc aggtaatgag
     2761 atgtataaaa cggaatttgc aaaaccattt ccgataagca tgcccacgag tcctacggca
     2821 aaaaaggaga cgaccattga aataagtgca acgggaataa aacgaacaga agctttcatc
     2881 aatagattac ggttcatacc aaggatgcta ccagtaatca aggcagcaat ataaaagtcc
     2941 caaaagccca tattgctgac aaagttttct gtggatttca ctacatattc aggtaagatc
     3001 ccaaatgtag caatcgcagc agaagcgaag atcgcaaaaa cggaaccacc acccaaataa
     3061 cttttgacga tagggatttt tgtcccgata aaatgaaata aattaccgaa aataaccaaa
     3121 actgcgattg gtccaatcat attcgtcggt aatttcccta aagccattgc aatgatcagg
     3181 acaaggacca tgatcaaata aagtggcaaa cttacgccgg tgatttttgt ggaccaaaaa
     3241 ccttcttttt tcttttcctc aactgcagtt gcagttgttg ttttttgtgc catttttaca
     3301 gatcctttct tattctgtat catatgaaaa aagccaaaga aaagcgacaa aagagcagga
     3361 gttggctggc cgctgttttg ccgcagtcgc ttttttcttt ttagacgtca tacaaagttt
     3421 ctgtatgttc ttttactata acgatcttaa tcttctaaag atttatattc aggaacccat
     3481 ttagcagatt caactgcttc tttgatatcc gtgatttctt cacgattcaa gttttgtgcg
     3541 acaactgatt ttgcgacagt ttcagcaatc gtttgagaaa attcagtgat ttttgctact
     3601 ggaggcaaga tagctgctcc tggttttgtt acatcaacga ttccgcctaa tgctcgacta
     3661 gcttgagaaa tgatttcagc atttactcgt gtagctgttg aagcaatcaa accaagtcct
     3721 aaacctgggt acatcaaagc attatttgct tgaccgattt ggtaagttac accattatat
     3781 tcaacatcag cagcagggat acctgttcca actaatgctt ttccgtccgt ccattctatt
     3841 aaatcttttg cttttgcttc tgccaatttt gttggattag acaaaggcat gatgatcgga
     3901 cgtggtgtat gagcagccat ttctttcacg atttcttcag tgaatgctcc aggttgggta
     3961 gaagtaccaa tcatgattgt cggatggatc gcttttacaa ctgcttctaa gtttgtcaat
     4021 tcatcagcat tagtgaaatc tgagcgtttg cgagcgaatg ggatttgacc aggagtcaat
     4081 ccttctgtat cttcaaacaa taaaccttgt ttatctactt gatagaaatg ttggcgtgct
     4141 tcttcttcag gtacaccttg acggatcatc tcatctaaaa ggatgtttgc gatacccaca
     4201 ccagcagtac cagcaccaaa tgtcaaaatt atttgatctt tcaattgttc accggatata
     4261 ttcaatccgc ctaatacacc tgctaggaca acaattcctg taccttggat atcgtcgttg
     4321 aaagtagtga tttgatcttc gtatttttct aagatcgctg cagcatttcc tcgtccaaaa
     4381 tcttcccaat gcaataacaa ttctgggaat aattcggtag ctgattgaac gaattgatcg
     4441 atgaattcat agtaagtttc gccttctaca cgagcatgac gattgcctaa gtataaggga
     4501 ttattcaata attcttcatt attagtacca gcgtcgatcg atacaggcaa aacttgtgct
     4561 ggattaatac cagcagcagc agtatagacc atcaatttgc cgatagcgat gtcaacaccg
     4621 ttcacacccc agtcgcccat acctaagatt ccttctgcat cagtcacaac aatcaaacga
     4681 atgtctcgtc catctgccgc gttcttcaaa cttgctttga tgttttctgg ttcgtcgatt
     4741 gaaagaaatg cagcatcttg cggtttcaag aaaatttcgt tgtattgttc gattgaatcc
     4801 gcaacgacag gatcataaac gacaggcatg aattcaacta aatgttcgcc catcaattta
     4861 taaaacaatg tccgattcgt attgaataga ttcattaaga agatacgttt ttccaaatct
     4921 gtttgtttgc ttaagtattg accataagct tgaacagatt gttgttccag tgtttggaca
     4981 gtacttggaa gcataccagt gattccatat tttgcccgtt cttcttttgt aaaagctgtt
     5041 cctttattta gaaaaggatt atttaataag tttaaccctg taagcatgaa tgattcctcc
     5101 taagttttaa tacaaacgaa gtatagaagt cagatgatga tatagtcaaa aagagcaggt
     5161 atgataaatt ttatttatat gtaatatagc caaaagaaat caaaatagca gtatttgttc
     5221 tttttatatc gacaatcctt gcttgaatac tatgcgtatc tcttatatgc tagagaaaga
     5281 aaagtagaag aataagggat agatggaatg gataaatagg atttctgagg aaaaaagcac
     5341 atgatagcgt tgataggagt cacctatgtc aggtgataaa aatacataag tagaaaatta
     5401 agaggaggaa gagaaaatga aagaagttca attgattaga aaaagcgaac tatcagaagg
     5461 cggttgcaat gcatgtggcg tagtagaagc gacaagctat acattaaaat tagattccaa
     5521 tcaagcaata atctctgaac tgacagtagg cggtcttgtg gacagtctgg cgcttgctga
     5581 aggttttact ggagaagata tttatgagat gttttcagaa gtccgtcagt taaaaaaagg
     5641 agaaaaatgc atagaggttc atcatgagag cccgaatgtg aggttcaaac gtggtgataa
     5701 tgagatgatc ttcaagaatc atgtgtctga tcacactgaa ctatatgaaa tcgtgaatca
     5761 aatactgtct gggatttttg aattaggacc ttatgagttc aaggaagaaa acggtaatcc
     5821 aaaattaaat gaagaatggc aagaaacgat tgaaacacaa agaaacaatc cacacttatt
     5881 tcaatagtct tccacagtat ttcgtaaaaa tgttgatgaa aaagaaacac cgtgaatcag
     5941 aggtcgtgaa aaacctgccc tgcatcaagt aataagaaca gccaaacaaa aatagctcct
     6001 cctatttttc gtttggttgg gcttattacc gcagatgcaa gtttttgaac accgtttaag
     6061 gaagagactg tgaagtcaac gttctgacct gagtattaga gagacaaatg gcaaaatagt
     6121 tcctcatatt ttgccatttt ttgagctaat accgagggtc agctgattga acaccgtttt
     6181 atcgagaggt tgtgaaaaag tcgcttagtt tcgagtagta agaaaaattt tgtgaaaatg
     6241 gctttccaca tttttgcata aatttgactt aataccgaga aaatggcttt tgaacaccgt
     6301 ttattcaaaa aaagggactt tgacactact tttgtcaaag tcccatcggt tttttatcta
     6361 tttttattac aatcatttct tccagagtag tgacatctgg aatggctgaa atgtaaacag
     6421 attatttgcc tgtatcaatg tcgaagagta atataaaaag tgtaaacgct atcatattgt
     6481 tcatcattca aattttatag agagaaggca ttgagatgga aaaaacatat caagcaggta
     6541 caaatgaagg aatagtagat tttatcaata tggaagattt agaaatcgcg gcttcacaag
     6601 tgattccagc tggtggttat ggttacatca gcagtggtgc tggcgactta tttacttatc
     6661 aagaaaatga acgggcattc aatcatcgtt tgattattcc tcatgtatta agagatgtag
     6721 aattacccga tacaactaca cattttgacg aagaaatgct gactgcacca atcatcatgg
     6781 caccagttgc tgctcatgga ttagctcatg tgaaggcgga gaaagcttct gcaaaaggtg
     6841 tagcagattt tgggacgatc tatacagcaa gttcttacgc ttcttgtact ttagaagaga
     6901 taagagaagc aggcggggag aaagcaccgc aatggtttca attttatatg agcaaagata
     6961 atggaatcaa tttggatatt cttgaagtgg ccaagcgtaa tggagcaaaa gcaatcgtat
     7021 tgacagcgga tgcaacagtt ggcggaaacc gtgaaacaga ccgcagaaac gggtttacct
     7081 tcccgctgcc aatgccgatc gttcaggctt atcaatcagg agtaggccaa acgatggatg
     7141 ctgtatataa atcttccaaa caaaaactta gtccaaaaga tgtagaattt atcgctgctc
     7201 attctgattt gccagtctac gtgaaaggtg ttcaatcaga agaagatgtc tatcgatcat
     7261 tagaatctgg tgctggtgga atttgggtat ctaaccatgg tggccgtcaa ttagatgggg
     7321 ggcctgctgc gtttgattcg ttgcaatatg tggcagaagc agtagataaa cgtgtaccaa
     7381 tcgtgtttga cagcggtgta cgacgtggac aacatgtttt caaagcaatc gcatcaggtg
     7441 ccgatcttgt tgctattggc agacccgtta tttatggatt gtctttagga ggaagtacag
     7501 gagtccatca agtgtttgat ttctttaaaa cagaattgga aatggtcatg caattagctg
     7561 gaacacaaac agtagaagat atcaaaaaaa taaaattgcg tgagaatcgt tttatttaag
     7621 aaataaatga cgaaaaaaat aggagtgcgg cagatgatgc cgtactccta tttttatagt
     7681 tcaaggatgt cactaataga ataatttgaa acgaaaaaat aaaattgaaa tattttttca
     7741 tacttaataa aaaaggaaga atattcatgt aaacgatttt attgtttttt tctcttttat
     7801 acaaaaaggg tcataatttc aagtaagcgg atgaattcat gaggattttt tgcatactgt
     7861 ctctactata cttaggtcac taagaaaaag gagtgattta ttatgaaaaa gaacatgatt
     7921 gggacattat tactttcaac acttcttcta ggcggcttgg caacaccagc ttttgctgag
     7981 ggtcaagcga cttcaaaagg agatattacc tttactgaac caacaaatac cgttgagcct
     8041 cttaacccta ctgatccttc aaaaccagta gagccagcag atccggaaaa tccagcaact
     8101 ggccagacag gttctttaac attggatgta gttcctgaat tgccatttgg tacacacgag
     8161 attgaaagcg gaacaaaaac gtatcaagta gacgcttcaa aaaacgatac accttatctc
     8221 caagtatcag atcgtcgagg tgtaggtgca gatggccaag cacaagggtg gaacgtgaca
     8281 gtttcggttt cagattttgt aaacggttca caagttttgc aaggcgctga attagatttt
     8341 ggaacatcca ctgttaaaag tacgtcagat aacgaatcca ctggaccaac tagtcaaact
     8401 gtaactgggt tgagtaaagc aagtgcagcg acaccaatat ttacagcagc aaaagatcaa
     8461 ggtctgggaa catggctttc tgtttatgat ccagcaaata tcacactgaa agtgccgaaa
     8521 gcagcagctg gaacattcac ggctgatttg acttggaacc tcgtggctgg cccagtagca
     8581 tagagtatga aataaattca acaaactgcg gtgaaaatac tataggtgaa ggatttttta
     8641 ccgcagttgt tgttgaaagg atgtggtgga caatgaatca aagtaaacga ctgtttgttt
     8701 cgtttttttc tattttaagt ctatttttca tttttccgtc aatcagccaa gcaaaggatt
     8761 cagcaggaga ctttggaatc aaaccggtat ttccagagaa ccaaatagat aaagcaatcg
     8821 gttattttga tttattagtg gcaccggaac aaaatcagac actagaagta attatcagca
     8881 attcttcaga tgaagaacga acatttgagg tctctgtcaa tccggcggta acgagtgatg
     8941 gtggaacgat cgattatagt caaaaaaacc caacgttaga tgagacattg ccgtttgatg
     9001 tccgtgacgt tttgctcatt gcgaaaaagg aaatcaatgt atcagcacat gcagaaacaa
     9061 ctatacctat agaagtgaaa atcccagcaa aaagctttaa gggacgtgta ttagcgggta
     9121 ttcatgtttc acctaaagaa gaagctgaaa cagaaaatgc tacagaagga gcacaaatca
     9181 aaaatcgtat tgcttataat ctcgcagtcg ttttacaaga aagtcaagag acgatagaac
     9241 cggatctgaa acttttatca ggagatttgg acgaggtcaa cgcaaagcca actgttcagc
     9301 ttcgtttcca aaatcctcag cctaggatta tcagcaattt gatatttacg agcaagatct
     9361 tttatgaaaa tcagctttat attgaaaata cgtctaatga atttttagtt gcaccaaatt
     9421 cgaattttca cttgaatctt gatttagctg gtgataaagc aaaagccggc gattaccgcg
     9481 cagaaatcat agcaaaatca ggcgacagta atgaatggcg atttacacaa aactttacga
     9541 tcaaaaaaga aaaagcacaa aaagtaaatg aaaattccgt ttttgcagta caagaacaat
     9601 ctttcccatg gctttatgca ggattggttg ctgcagttct agtgttatgg ttgattttgc
     9661 tgtttttgtg ggtccacaag aaaaagaaaa aaggagaaac aacatgatga ggtgatttcg
     9721 ttgaaaacat cgatcttatt gggagggatt attgcaagtt tgctgttctt cagctggcaa
     9781 gagccagtcc aagcagatac caaaagttca ggtacgatta cattttatga tctaagtcca
     9841 gttccgcatc caagtggttc agtcaatggc gcatcaaaac agcagacaca gctaccgatt
     9901 tataaagaat ctaatcaaca tggcgtattc ccggagacga atgaagaaaa gatccgtttt
     9961 ctttatttga tcattggtat agaattatgg cttcttgcgt ggttgttgtt atggagaaga
    10021 aaggaaaagg aggatgaaca tgttgctaca aaataaattt tcattttttc gctccttctt
    10081 ttttacttcg ctttttgttt taagtatatt tatcttttca ccttcggcag aagctgttgg
    10141 agcaacgcct cctgccattt cttatgatcc atacacgtat gaaaatatcg tacctccaaa
    10201 tggtggacgt agtgcagtca atggcgggaa gcttgtccaa ctaactgggg ctaaccaatt
    10261 gcctaaattg actgctactt atttgaacga tggttcaaat gatccttgga aaggtggcac
    10321 ggtccgtttt cattgggaaa gccatgtgaa actaacaaat gtacttgatt tggtgaatat
    10381 cactgcacaa ggatttgcat ttgtggcgat catcaatttg ccggaacatg tatcaagcag
    10441 tgatgtatta aaagcaattg actggaacaa cgcctacttg tatattgccg gtgcccccat
    10501 caaggttact agcggtagtt tattatcagc aggaagccat accttgcgtt ttgggatggg
    10561 gtcgtgggat acaaatactc tattggcaga tattattgct attattacgc gaggcgaatt
    10621 caacgtggac gatatcccgc ttcaatttga tgttacagct aatgtcgcga agatgacaga
    10681 aaacggagat aaatatgata cgagtccaaa caaaattttg accaagggta aattacaacc
    10741 ttctatgtct aaaaaggccg ttttttctgt tgatttttat gattcgaata atattatcca
    10801 agatcgtatg ttcaattcga ttttagctcc agctgcaggt agattttatc ctaaattaac
    10861 tggcggaaac ttgaatccag gagcatatgc attttctgca agtgcagaga taaatacgtg
    10921 gaatagttac atctcaacaa gtgattcaac aggaaaatat aatacatttg ctgataccac
    10981 agaatacgtg gatggaaata accagaacct tccaggctct accaatagtc ggacattgac
    11041 gatggggctg actgaaggac gttttgatca gttgccattt agtcgctttg atcgcgtagt
    11101 caactatttt acaggaaaaa acgttacaca aggagcaata ctgactcata ctccagcaag
    11161 cacaagtgga ttgaatcaga cagccaatat cgtgtacagt gggaaagatg cgcaaggcgg
    11221 attgctttct cctgttgctt taaaggtatt ggagcagtac aagctagacg gaacggtttt
    11281 accaacaaac ttaacagccg atagtcagtg gggaaagctc ctttcttctg catcgtacca
    11341 agtcacggcc aattggtcag caactgaact aaaaaagggt tcgatccatt acaggcttta
    11401 ttcgaaagga acccaaaaat tagcgacagg atggacagat caacttttcc aaacgatcca
    11461 atcaaataac gggggtgtaa atacagctac ttctacacta cctgcattgg cttcagggca
    11521 atattatttt gattatcgct tggtggacga tgtacttact aacatgtatc cgtcttttgc
    11581 gtacaaatgg cagtctgaac aagctggaat caccactctt cctcaaataa cagtggctaa
    11641 ttttccatct gtgagtgcag atagtcagct aaaaaacctt agcagacaac ctgagacagg
    11701 agatcccctg tctgctctat caggagatac gattcaagaa aatgtgacat tcacgttaac
    11761 aaaatctggg gatgcgataa ccgacaaaaa aatcacgatc tcacttcctg aaaatactac
    11821 ttatatcaat ggttccttaa aattaaatgg tacaactatc gcagataccg gcattcaaca
    11881 aggagtttct gtcccagcag atctattgag caaaatagga gataccattc atcttactta
    11941 caactatcaa ttaaatactg tagatacttc tgtacaatcc gtcagcatac tgacaaaggc
    12001 agctgtactt tccagcaata ttacattagc agatggtgct aagcttccta acccagttgt
    12061 ccaaacatct gctaaaacaa ttcttgtccc caaacaagaa ttaacattag ttaatgtacc
    12121 agatgatttt acatttggta acgatctccc caaaccgttg aagacaagtt attatgaagc
    12181 aaaaggcgat ttttcttttg atgtgcggga tactcggctt ccaagcacgt ctccttggca
    12241 actgacaggg acattgactt ctttatttaa gaatgatcaa ggtcaagaat taagcggaac
    12301 aaaattatac ttcaatcatt ctggatcaaa acaattgatt cagcaaggtc aaaacacgtt
    12361 gatttatgag agtgatggaa cagctaaagg agaggttctc gttgactttc ccgataccga
    12421 tggtttactc ttagaggtaa atagcagtac gaatgctcaa cctggcgcaa cgtatcaagg
    12481 catggtcaca tgggaactga cagctgggcc aaccagctaa gtgattagaa tttgtatgga
    12541 tctgtgataa ctgtcacaga tcctcttttt caaggatgag aaggaaactt ctgctgttaa
    12601 aatcagaaat gcttggagga gtggacttgg aacttttcag tttgcttgaa aaaaatgaac
    12661 aacggcaaat caaacttttc caacttttat tagaggaaaa aggagagatt tccgttaata
    12721 acgtacttac ccgcttatca gttgatcggg ctaccttgaa agaagatctg ttttatttac
    12781 aagctaactt ggaaaatatc aaacaattgg aaattgttat ccaagaaggg aatattttat
    12841 taatcagaca tggaaatata gcagcagtcg atgtttatta tcattgctac ttgcgctatt
    12901 ctacaaaata tcagatactt ctttatctgt tggaaaatgg ctcatttgat cggcagaaac
    12961 ttgtcatgga acttaatatc agttcggcta ctttgtctcg cagagtgaag gaattaaatg
    13021 attctttaaa tgagtttcat ctgaaaataa aaagcgggcg gctgaccggt ccagaaagtc
    13081 aaatttgtta tttttatttc caattatttt ggtttggacg tccttatctt gaaaacaaaa
    13141 aagaatacag tgatttatca ggaaacactt ttttagatct gctgcaaaaa gagtttagtt
    13201 ttactattac agaagaagga cgattgaaat ttgaaatatg gctttccatt atgaagaagc
    13261 gaatgcgctg tacgaaaaaa tccaaaccag attatagcaa tcttcctgtt ggattgtttc
    13321 aaacgacaca atttttattg gacctccata catttttgac ccgttacttc tttcaatatg
    13381 cctttccttg gaatcaaaat gaaacgttgg ttttttatct tttcatgatc agtaacttta
    13441 cattagatac acataactcg catgtccagg agtttttgat aagcgaaaaa tacaaagacg
    13501 acgtagtagc tcaattgaat cgaacgttta aacaatcgat agaagaatat ttctcttttt
    13561 atgtttttcc agaagagatt tcgcaaaaga ttgcgttgac agtgatgcaa aatcacgctc
    13621 attttgccta ttttagagga tggatgaata tttttggcca agaaggatta aaacagcggt
    13681 taactgaagt agaagataaa caattcctgg ttttatgtga aagattggtc agacaaacat
    13741 ttgctattgt tgattttact gaagtagacg aggaaaatgc ccgctttgaa ttatttgctc
    13801 gctatgcatc tattttgcgg acgctgatca agcagttaga attacgtctg caaattgctt
    13861 atgatcttcc ttatgaaagg ctaatggcgg atatcgtgaa agaaatcatc caggaacaat
    13921 tgctgaatac gatcaaatat gatttagtcg aatacgagaa agaaaagcag tatgatatca
    13981 ttttgactag ccaattgaaa gagtacccgt cacagaagtc cgctaaagtt tttgtattca
    14041 ctagcaacga atataattat gatttcccct atttgaacca atttttaaaa gaatgttatt
    14101 tagaaaaatt gaatttaagg atgaataaaa catgaaaatc gtgatcattg gtgcttccca
    14161 tgcaggcatc acagctgccc taaatctaag aaaacttcaa ccgaaagcag aagttttact
    14221 gatcgatgaa gatcataaag atggactagg ctatgtttca aatggaatca acctttattt
    14281 aaaaggaaag atacgtagtt tagctgaagt tgcccataac atgcggactc ttcaagaaag
    14341 tggggcaaaa ctgatcaccg aatggagagt aactgaactt gatccagaca aacatcagct
    14401 catcctcgca tccaaagaag gaaaaaacga gaccataacc tatgataaat taatcgtagc
    14461 aacaggttcc tctcctgtca ccttgtataa acaaattgaa gctgaaaatg tctacaccta
    14521 taaaaatcta gtgcaatcta agcaagtact tgccgcttta aaagaagcaa aagaagtagt
    14581 gatcttcggt gctggttata ttggattgga attagcagat gctttgagaa ataaaggata
    14641 cattattcat ctagttgatt atatgccgaa tgttttatca agatatttcg ataaggatat
    14701 gatcaacagt tttcaaaatc agctgcaaac taagcaaatc aatttttatc caaatgaatt
    14761 cttgatcgat tggaaaaaat cagaagagaa agtagtatcc gtacaacttc tatctcaagc
    14821 aataaaggca gatatggtca ttttttctgc ccaaactcgt cctaatacga cattgttaaa
    14881 agaaaaagtg gctttgtatg aggatgagac tgtgatagtc aatgaatatc tgcagacaag
    14941 tgatccggac atctatgcga ttggagatat cgtcccagtc agttttgaca aaaataaacg
    15001 gcatctcttt ttacctttag ttactcgagc tgtacatatg gcaagagcag tagcgctaac
    15061 tttgagtggt cagccaaccg cttacgacct cagacaaaag ataacagcag cagtcattat
    15121 agactacttt ttaggaactg tagggctgac agaagatgaa gcgccattct tggaacaaaa
    15181 taccggttcg tgttcaggag aatttgatct atttccacaa tacgatgaag aaaacaaaac
    15241 agtaaatgca aagctgatct accatccgga tacattagaa ataattggcg gacagctgat
    15301 aagccaagaa ttcttattat cagatttgaa cctgcttgcg gatatcgtga aacacaaaac
    15361 tacgattcca cagctagcag ttgaggattt tggatttctg tctgaacaca cctctcgttt
    15421 tcactacctc aatgagttag cttttaaagt actagctaag agagcaaata gcaatagagt
    15481 agataaaaga ctgtgagaaa tatcacggtc ttttattgtg tggggatata ataaatatta
    15541 atcaatcgag aataaaaaat tcatataatg aaatagcgga agaatttttt gagcataata
    15601 ttcgccaaaa atttatcaag tgataaaatt gttgtataaa atgattattt caggaggagg
    15661 agttaaatgt ttttagcatt aaatgaaatt atgcattcaa agttacgtta tgcgttagtt
    15721 gcaggggtga tgtttttgat cgcctacctg gtattttttt tgactgggtt agcttatggc
    15781 ttagctcaag ataatcgaac agcagtcgac aagtgggagg cagattcgat tgtgctttcg
    15841 aaagatgcaa attcaaatct agggatgtcc atgattacta agaagatagc agaagaagtc
    15901 gaaggtggga aagtggcgta tcttgcccag acacctggag tcgtgactag taaagacagt
    15961 acagaagaag gaaaaatcaa tgttagcttc ttcggtatcg ataaaaatca attcatcatg
    16021 cccaatcttg ttgaaggaaa agcttttgat aacgatgatg aggcagtagg cgatattagt
    16081 ttgaaagaag aatatggtct agccgtagga gatacagtta agttatctgg tagtgacaag
    16141 acgtttaaac taactggctt tacggatcat gccaaattca atgtctcacc agttctctat
    16201 acaaccatca atgcttacca agagatccgc ttcgaaaaag aagatacaag tgaaaatgca
    16261 cggattaacg caatagttgt cagaggtaaa atcagtaatt tacctgaaga tttagaacag
    16321 atcaaaattt ctaaattcat caatgaatta cctggatata atgcgcaagt attgactttt
    16381 ggatttatga tcggattttt gatcgttatt gcggctattg tgatcgggat tttcatttat
    16441 gtactgacga tgcaaaagat taatatcttt ggtgtaatga aagcacaagg gatcacagga
    16501 ggatttattg cgcgttctgt agtggcacaa acattcattt tatcatttgt tggtattctt
    16561 cttggtctat taggaacagt gggaacatcc cttgttctac ctgatgcggt tccattccaa
    16621 agcaattggc tgttttttgg cgtcattagc ttgttgatgc tggtagtagc tgtgttaggc
    16681 gcactatttt ctgttcggac aatcgtaaaa atcgatccgt tgaaggcaat tggataagaa
    16741 ggaggaagag catgaaagca atagaattta aagaagtaga aaaaagtttc caagatggcg
    16801 accaagtagt taaagcattg aaagaaacaa ccatgagtat cgataaaggg gaatttgttg
    16861 caatcatcgg tccttctggt tcaggtaaaa gtactttttt gactttggca ggcggattgc
    16921 agacgcctag cagtggctct gtatttatca atgatcaaga attcagtaat caaaaagaaa
    16981 gtgaacgcgt taaactacgt tttaaagaaa ttggctttat tttgcaagct tccaatctgg
    17041 ttccttttct gacagtaaaa aagcagttat tattagtaga taagatcaaa catgaacatc
    17101 gtcaaaaaga agcaaaagaa ttatttgagc agcttggcgt ggataagttg atcaacaagt
    17161 atccagaaga actatctggt ggagaacggc aaagggtcgc aatcgctcgt gcattgtaca
    17221 atgatccgtc cattattcta gcagatgaac caactgctag tttggattca gaaaaggcac
    17281 gagaagtagt cgatatcttg gcaaaagaat caaaagacaa aaataaagca acgatcatgg
    17341 ttactcacga tacacggctg attgacaaat gcgatagaat atttatgatg gaagatggtg
    17401 tattgaaaga aaaagaataa ccaaaaagca ggtggaaaaa tggtccatcg atacaacaaa
    17461 gatacacagc aaaaaatttt gaaagaagcg actgacttgt ttatgtccaa gggatatctc
    17521 ggcacttcaa caagagagat cgcacaaaaa gttgggatca cccaacccaa tctctatcat
    17581 tatttcggtg ataaagaaaa actatatacg gcagttttag aaaaccactt aaaagatgtt
    17641 ggaaaagctt tgagagaaat cgttcaaaca agcgaaacgg gatttcaagt aaccttgaca
    17701 aaaatggcgc agtttttgat tgagacacat ctggtcgatt tgtttctgat gcttcacgat
    17761 cttgaaagca atctttcaaa ggaaacaaga gatcatcttt tctttctatg gaagaaaaat
    17821 tatcgtgaac cttttgaaga aatcttttca gcaaaccaat ctgtgctaag aaatgggatt
    17881 tcaaaagaaa tcgcagcaag gcattttttc ttagtgttag caccttacat cactcggtcg
    17941 aaaagacctg tggaagaacc gctaaatgtg gcgcaattga tcgatttata tttgaatggt
    18001 gtattaggag aagcgtaaag ctgttatata gggagaaaaa tagttaggtg aagaagcaag
    18061 atatgacgaa gatcgttata tcttgttttt tttatatagg tagtttttca taaatacaaa
    18121 tttgttattc tctcatgatt atcagggtat atttttattt attatgtcgg tgttccagga
    18181 agtgttttaa tttaataacg aagaaattaa ttgtttgtag aatgaaggaa actatttatt
    18241 gggtagatga atcaccacga atagtcaaaa ggagatttca ggatatgcca aaaatgaaaa
    18301 aaatcatttt agcagtaatg ttattttcta cggttatatc ttttacagcc ccttttttgc
    18361 gtacttaagc tgattttaag ttttgaccct tagtctggat ctacgaggtg agagtcaaga
    18421 atgaaaaaaa taattggagc ttttgtattt gcaatcatta tcattattat ctgttttcaa
    18481 atagatgttt ttggtcaaaa aaagatagtt ggacaaacgg tcagcagtac gaacagtacg
    18541 gttacaaaga caaaggaatc atttgatgat acagaacaag agacattaat caaaatcgga
    18601 aaaaatgacc gtacactata tacagatgac tcgctaacta ccccacttgc aactataaac
    18661 ggcggagagc tggcggagtt tctttctgaa acaaaaaatg cttatcagat caagacaaat
    18721 gatggctata ctggatattt agccctcgaa gatggaacca aagtcacaaa gaatatacaa
    18781 acaaaaccgg aagagctatc tgatgcagtg attgtattag atcctgggca tggcggaaat
    18841 gatactgggg cattaagcaa tgatgaacaa acagaagaaa aagatataac attaagtaca
    18901 gccataaaag taaaaaaggc gctagaggat gctggagcaa cggtttattt gacccatact
    18961 acagatgaat tagtacagct tggagatata tgcgactaca gtgaagaaaa aaaggcagat
    19021 gtctttatca gtttacacgc agactctacg gaatatgcga atgaagcaac tggaatcacg
    19081 acatattatt actatggaca agaggaaaca ttagcacaga caattgctga ctccttcact
    19141 gatctgcctc tggattcaag aggaatctcc actgggaact atcaagttct tcgagaaaat
    19201 cttcaacctt ctattttaat cgagatgggt tatatgaata atgatagtga tttaaaggaa
    19261 ttagtaacag acagttatca gcagcagatt gctgcaagtt tgacacaagc attgaccact
    19321 tattttcagt aataaataag cccagccaaa caaaaatagc tcctcatatt tttcgtttgg
    19381 ccgggcttat tatcgcagat gcaagctttt gaacaccgtt taaggaagag gttgtgagcc
    19441 tgccgtttaa ctccgcgtca caaggagcaa tttcaaaaaa cagcttctca tgtttgatga
    19501 ctattgtgac ttgtgaccga ggagttggca tgagaacacc gtttattcgg aacaagggac
    19561 tgtgacatga cttttgtcac agttccatta tttttttagc agcttcaaag ctttttctgt
    19621 attggcaaaa tgaagtttgg catatttttc aagcataggc atgccgcctt tctccaatag
    19681 ttttgccgct acttggtcag atttcgttcc agctcctgat ggaagggtga ttccagcttc
    19741 agctgatgct ttgtgtaact cttccaaaaa gctggcacga ctgatgatag aagcagcagc
    19801 aacagctaaa tgatattgtt ctcctttagt aacgaagtac agtttttctt gtaattgatt
    19861 tttttctaaa cgcgcatatt ttttaaaatt tgcttccggc gtaaactgat cgatcaaaat
    19921 cgcttctggt ttttctggct cgattttttt tagcagcaaa tggatcgctt gattgtgcaa
    19981 agcgactttc atccgaacgg cattgtattt tggttggatc tcattgtatt tttctggaga
    20041 taagacaagc agtttatagg ggatggtttt ttttaagatg tcagcaagct ggatgatttg
    20101 gctatctttt aactcttttg aatctctaac accaagttta cgtaattctg caagctgatc
    20161 acgtttcaca taagcagcac atactgttag cggaccaaaa tagctgccat ttccgacctc
    20221 atcactacct aaaacggata attgactgat attttttggc aaagaagagg ctgtttttgc
    20281 tggcttggct tgtttatcct gtgcctcccc ccatttatca gcttctattg ctgcattcgt
    20341 accttgaaac atcacttttc cggatgtata agcggtaatg gtcgtcccgt ctttttttgc
    20401 agcaaagcta gtataaggaa tctgtttatt taacaaataa gagcgatagt atgctttcat
    20461 ttgactaagt tggtcgtttg ttgctttgat aatgacgttg ctcataaaat tctcctcgtt
    20521 tttatacata aaactatacc gcaactcacg acttttcgtt ttctaagaga ttgcgagaca
    20581 tagcattttt ttaaagttta tcatataata gttaagtatg tgaaaaatat ttgtggagaa
    20641 cggtgaaaga accaacttca tggtacacta aataaaactt tcatcaaata ggaggaaatg
    20701 attcgtatgg cgcacgaaaa aacacgctac aaagcagtga ttgccaacca aacatatact
    20761 attatcggac gggaaaccaa gcatcatatg gatatcgtaa cgaagctgat caatgaacag
    20821 ttagcagaat tgaaacaatt atcaccacaa atggataatg aacaagcagc aatcttgatg
    20881 gctgtcaatg cgctttcgga tcaattgaag aaacaagaaa gaatcttgga gctggaagaa
    20941 gaaacagcag aattaaagaa aaaaatgatc aaatttacag agcttgaaaa tcgtgtgaaa
    21001 cgcattgaag caatcgagaa cgaagctcgg gaagtattga aagaaaatgg tcaagcagat
    21061 cataccatta aaaatcatgt ggaagcacaa cagattttaa acgagaaacg aaaaggacaa
    21121 attaaacaaa aatcttctaa ttaagatcct tttatcaaaa taagaaaaat ttaaaaaagt
    21181 tagaagagaa aggaacgtag catgctgagt ttgctgattt tatttttatt gctaattgcg
    21241 tttttttctg gagcacgtcg cgggtttgct ttgcaaatca tattcgcgat tggatatgta
    21301 ttgtcattta ttgctgccca gcatttttac aaaccattag caagtcattt ggaattatac
    21361 attccatacc cagctgtcac cccaacatca aagttagcat tttttgatca ggtatttgct
    21421 tttcacctag atgaagcttt ttatgcagga actgcttttc tgattatttt gctaattggc
    21481 tggctgctta cccgatttgt gggggttttt gtccatggat tgacgtatgt gccgatcttg
    21541 agacaggtag actggattgc aggaggcatc ctgtcactaa tcatggctta cgttactata
    21601 tttttgattt tgcagttact tgcctttgtt ccattagtgt ggtccaaaat caattcggcg
    21661 agcatagttt cgctcgtttt atcgttgaga gaacgccatt tcttaccaac aagatgtatg
    21721 agctttgggt aacgaatatt ttgaattaag gaaaatagtg ctggaaaata tttcgttttt
    21781 tgtacagagt agtttattta agaaggctgc gtagcgtatg tccggcacag ctcttttttc
    21841 atagtgaaag ataaggtgat aaaatgaata accgaatcct tgaaacattg gaatttgaaa
    21901 aagtcaaaca gatggtcaga caatttgtag tgactgcaca aggaaaagaa gaactggcag
    21961 aattggtacc agtgagtgag aaacaaacga tcacaaactg gcttcaagaa actgaggatg
    22021 gattgaaagt gcaacgtctg cgaggcggga tccctatacc taaacttgaa aatatccgtc
    22081 cccatatgaa acggatcgag atcggagcag acttgaatgg agtggagctt gcacaagttt
    22141 ccagagtatt gtctacgaca agcgaattga atcgtttcat tgatgatctt tctgacagtg
    22201 aaatcgaatt tgcacgtttg tatatgtggg cagatcagtt agtcacgatc cctgttttga
    22261 gccgccgttt aaaggaagca atcgatgaag acggacgcgt gactgatgac gcttctcctg
    22321 agttaaagag cataagacag aacattcggc gaagtgaaca agctgttcgt gaacagttag
    22381 atgggatcgt ccgtgggaaa aatgcaaagt atttaagtga tgcgatcatc accatgcgaa
    22441 atgaccgcta cgttatccca gtcaaacaag aataccgcgg tgtatttggc ggtgtagtcc
    22501 acgatcaaag tgcttcagga cagactttat ttattgaacc aaaacaagtg gtggatttga
    22561 ataatcgttt gcgtcaatac caaatcgctg aacgaaatga gattcaacgt attttaagtg
    22621 aactttctgc tgaacttgtt ccccatcgtc aagaaatcat ccacaatgct tatgtgattg
    22681 gtaaaatgga tctgatgaat gcaaaagcac gatttggtaa agaagtaaaa gccatcgtgc
    22741 cgggaatcag tgaagacaat catgtcgtct tgaaacaagc aagacatcca ctgatcgatc
    22801 aggaaaaagt agtttctaat gacatcacaa tcggaaaaga ctatcaagcc atcgtcatta
    22861 caggacctaa cacaggtggg aaaacgatca cgttgaagac tttaggtctg ttacaactga
    22921 tggggcaagc aggattaccg attcctgcag gtgaagaaag tcaaatcggt atttttgagg
    22981 aagtatttgc ggatatagga gacgaacagt cgattgagca aagtctatcg accttttctt
    23041 ctcatatgac gaacactgta gatattttat caaaagtcaa tgaaaaaagt ctggtattat
    23101 ttgacgagct cggtgcagga acagatccgc aagaaggtgc tgctctagca atcgcaatct
    23161 tagatgatct tggtaaaaag tctgcttatg tgatggcaac aacgcattat cctgaattga
    23221 aagtttatgg atacaatcgg gcaaatacga ttaatgccag catggagttc gatgtggata
    23281 cactaagtcc aacgtatcgt ttgctgattg gcgttcctgg acgaagcaat gcgtttgaaa
    23341 tctctagccg attaggtctt gatacagaag ttattgatga agcgaagcag ttgatgaatg
    23401 atgagagcca agatttaaac gaaatgatca cagatttaga gaatcgccgg aaaatggcag
    23461 agaccgaata tctggaaatg cgtcattttg tttctgaggc acaagaactt cacgatgatt
    23521 tgaaagaagc ttacagctat ttctttgaag aacgtgaaaa agaaatggaa aaagcaaaga
    23581 aaaaagcaaa cgaagtcgtg tcagaggcgg aagaaaaagc agaaaaaatc attgctgata
    23641 ttcgtaaaat gcaacagcag attgggcaag gaaatgtgaa agagcatcag ttgatcgatg
    23701 caaagaccca attagcaaat ctacatcaag aagaaacttt gaagaaaaat aaagtattga
    23761 agaaggcgaa agaacaaaaa acgttgaaac cgggagacga agtgttggtt acgacttacg
    23821 gccaaagagg gacgctttta cgtaaaaacg ggaatcagtg gcaagtagaa atcggtatat
    23881 taaaaatgaa tgtgtccgaa gacgagctga cgcctgttgc tcctcaaaaa gagccgacac
    23941 aacgagttat ccatgctgta cgatctgaat ctagtagtca cgtgccgaat caattggacc
    24001 ttcgaggaaa acgatacgaa gaagcgctaa gtgaggtaga tcaatatttg gattctgcca
    24061 ttctagctgg ttatcctcaa gtgactattg tccacggaaa aggaacaggc gcacttcgca
    24121 agggaattac tgattatctt aaaaaccatc ggagcgtcaa aagctttgag tttgcaccgg
    24181 caaaccaagg cggaaatggt gcgacaattg ttaaatttaa gtaaagctta ctaaaggtaa
    24241 gcagatggtt cgctatccaa gtatagaaat gctatactaa tcatagattt taaaaatgga
    24301 ggtctttgat atgtcacaag taattacaga taagacattt aatgaagaaa cagataaagg
    24361 acttgtattg attgattttt gggcaacttg gtgtggtcca tgtcgtatgc aagcaccgat
    24421 tttggatcag ttggaacaag aatatgatga agaagaattc agaattgcta aaatggatgt
    24481 ggatgaaaat ccagaaactc ctcaacaatt cggtatcatg agcattccaa cattaatgtt
    24541 gaaaaaagat ggtcaagtag tagaaaaagc tgttggtgtt cattcaaaag aacaactacg
    24601 tcaaatgatc gatcaatatt tataaaaact tacagcaaaa ataagctgag ggtgggactg
    24661 aatccagttc cgtcctcggt ttttttgcgg ttgagttaag gttattcaaa ttaatatttt
    24721 ttgagtataa ttacgttgtc aggctggatt gtttttttga cagacatgct ataataactt
    24781 taattgaata tgaaggggag taccagatga acgaacgaat caaaaacaaa ttagctttac
    24841 ttcctgatca gcccggctgt tacttgatga aagataaaaa tggaacgatc atttatgtcg
    24901 gcaaagccaa aatattaaaa aatcgtgtcc gctcatattt tactggcagc cataatacga
    24961 aaacggaacg gcttgttagt gaaatcgaag actttgaata catcgttaca gaatcgaata
    25021 tcgaagcatt gctcttagag atcaatctga taaagaaaaa tgatcctaaa tacaacatta
    25081 tgttaaagga tgataaaacc tatcctttcc ttaagataac gaatgaaaaa tatccccgtt
    25141 tggtgattac tcgtaaagta ctaaaagaca aagcactgta ttttggacca tatcccgatg
    25201 tcggtgcggc gaatgagaca aagaagatcc tcgatcgatt atttcctctg agaaaatgca
    25261 aacctagtca gacgaaggag ccttgtcttt actatcattt gggacaatgt ctgtgtcctt
    25321 attatttcga cgtagatccg aaagtctata cgaaaatcgt tgatgaagtg aaaaagtttt
    25381 taaacggagg acacgaaaaa atcgagtcag agatccatca gaaaatggaa aaagctgctg
    25441 aaaacatgga gtttgaaaaa gctgcagaat accgagacca gatccgtgcc attgagacaa
    25501 tcatgactcg ccaaaaaatg acaaatactg atttactgga tcgagatgta tttggttatg
    25561 cgattgacaa aggatggatg tgtgtccaag tgtttttcgt aagacaagga aagctaattg
    25621 aacgagatgt ttcaatgttt cctttctata acgaagcaga agaagatttc ttgacttacc
    25681 tcggtcagtt ttatcaggaa aatgagcatt tcattcctaa agaagtcttg attcctgata
    25741 cgatcgacaa gcccagtgta gaagctttgt tagctacgaa agtgctgcaa ccaaaacgag
    25801 gagaaaagaa aaaattagtt gaattggcga ataagaatgc ctcagttgct ttgaatgaac
    25861 ggtttgatct gattgccaga aaacaggaac ggacgatcgg agcagttgaa cgtttaggaa
    25921 acgcgatgaa tatcccgacc ccaatacgta tcgagtcttt tgataactct aatatcatgg
    25981 ggacgaatcc tgtggctgcg atggtcgtat tcattgatgg aaaaccggca agaaaagaat
    26041 atcggaaata taaaataaaa acagtcaacg gaccggatga ttatgcatca atgagagaag
    26101 tcatctatcg cagatattca cgagtgattc gcgaagggtt gcctttacct gatttgatct
    26161 tgatcgatgg aggaaaagga caagtagatg tggcaaaaga tgtcttagaa aatcaattag
    26221 gaatcgatat tcctattgct ggcatggcaa aaaacgataa acataaaaca aatgagttgc
    26281 tattcggtat agatttggct gtagtaccgc ttgagcgaaa ttcaccagag tttttcttac
    26341 tccagcgaat ccaagatgaa gtccatcgat tcgcaatcac tttccatcgg cagctacgca
    26401 gtaaaaacag ttttgcttcc cgattagatg aaattgaaag gttaggacca aaacgaaaaa
    26461 aaatgctgct taaagagttc aaatcgttga aaaatatcac agcagccacg acagaagaat
    26521 tacaagaaat cggactgccg aaaaatgtcg cacaaaacgt atttgataaa ttacatcagg
    26581 aatgaggaaa gggcacttta caaagaaagt aaagtgtcct atcattttat gtacaacaaa
    26641 aagagcgttt acgttttgtt ggtaaataat aaatcgctta cgtctggtgt ctaaggatat
    26701 atgtgatttc ttgaagaagg agtgaagaaa tgttagaagt caaggatctt gttaaaactt
    26761 ttgggaacta cacagctgtc gatcatgtct ctttccaaat ccccgatgga aaaatcatgg
    26821 ggctaattgg ccaaaacggg gctgggaaga caacgacttt tcgtttgatc ttggattttt
    26881 tgacacaaga ccaaggagaa gtgttatgga atggccatca tcttggggag aaagattatg
    26941 atgtcatcgg ttatcttccg gaggaacggg gactttatcc gaaagtaacg attcaagacc
    27001 aactgcttta ttttgcagaa cttagaggga aatcaagaaa agagattgaa ccaaaaatcg
    27061 atttttggat ggaaaaattt caggtaaaag ggaaaaagac ggataaagta aaatctttgt
    27121 ctaaaggaaa tcagcaaaaa gtccagctga tcgccacgtt aatccatgaa cctaaattga
    27181 tcattttgga tgagccattc agcgggttag atccggtaaa tgctgaattg ctaaaagatg
    27241 gaatattgga attgaaaaaa catggttcat gtgtcatttt ttctagccac aatatggata
    27301 atgtagaaaa aatctgcgat catttgatca tgttgcgaaa cggaaaaatg gttttgaatg
    27361 gaaaagtaca tgagattcgt gaatcatttg gccgtacgaa gttattcttg gaatctggtt
    27421 taagccaaca ggaagtagaa gaaatcgaag gcgtgaaaaa agtgatcctt catgaagaca
    27481 atacgttaga gattacctta gaagatcctg aagctgggaa agaaatcttt actcgtgcga
    27541 ctcagttcgg ctacattcct atgtttaacc agcaaccgcc aactttggaa gagattttca
    27601 aattgaaagc aggtgaaatc gatgcgtaag ttttggatta ttaccaaaga tgtctattta
    27661 aaaaatgtta agtcgatttc tttcttgatc atgatattag tcccttttat catgctgggt
    27721 atcatttatg tagcaggaaa ctttgctcaa caaaatagtg atgtcaataa aatcggcgta
    27781 ttggctgata atgagcaaat cagtcagcaa atgagccaag tcaaaacaga tgattttgag
    27841 ttcaaagcag ttgattctga agaaaaagca caaaaagagc tttcggaaga aaaaatcgat
    27901 gcgtatttag tagtctcaac tgagaatgat aagataaatg ggatgcttta tagcgaaaat
    27961 tcattaggac aaagtacaca actactgata caacaacaac tgactggctt gcaatcgatg
    28021 cttcgagcaa gtcaattagg catttcacca gaagaagtag ctagcttgag ccaacaagca
    28081 agtttgactc gacaaaaagt caattttgat tcaaaaggaa aaatgacagt tggagaagat
    28141 aactcgacag tccaatatgt cgtaagttat gtagcaacga ttgtcttgtt cattattatt
    28201 ttgacgtatg cgcagatcat tgcacaagag atcgcttctg aaaaaggcac gcggatcatg
    28261 gaagtgatcc tgtccagtac gactgcacaa aagcattttt acggaaaact aacaggtgtt
    28321 ttacttgttg cattaacgca aatggcatta tacggtatta tttttggtat aggatacagt
    28381 caattcaaag atatggaaat ggtcaaaaat atattagacg gtatctcatt ggataacttg
    28441 attggaccat tcttgtggta ttctcttcta ttcatgatct ttggcatttt gattttttct
    28501 gttttagcag ctttatgtgg ctctttggtc aacaaagcag aagatacagc aaaagcaatt
    28561 ttgccagtga cttatctatc tctaggcgga tatatgcttg gattgatact aggagcttca
    28621 gatccaaaca atatcgttat tcggatcact tcgtatattc cattcttatc ttcatatatt
    28681 atgccggttc gcttagcaaa tgagacagtc ggtatgggtg gagcattgat ttccttgttg
    28741 gtacttgtag ccgtaacatt cttattgatg atcttgtctg caaacatgta caaatcgaat
    28801 gtactcgtgt atagcgaagg cggattatgg agttcattga aacaatcgat ttcaattatg
    28861 agaaatgaac gaaaaaaagc ataaaatata gaaaaatata gaaggaagct ggacatttat
    28921 tgttcccagc ttcttttttg tatagaccta tttctatttt tttattatat tggatatata
    28981 tattcattga aaatagagca taacaacttt ataatgttga gtataccaat ttttggaggc
    29041 gttttaatgg accgctattt agatttactt tcagagaagt tccctacaac ggaagatgtc
    29101 atcacggaaa tcatcaatct cgaagcaatt ttgcagcttc caaaaggaac agagttattt
    29161 ttgagcgata tacatggcga atttcctgct tttgatcata ttctgcgtat cggttcagga
    29221 aatcttaaag aaaagatcaa agaccttttc tctgaacaaa tgacggaaac ggagatcagc
    29281 caattcacta tttttacggc ttatccagaa tatgtgttga cgactgattg gtacaaaaaa
    29341 caagataaat cccagctgat ccatcgtttg atcgatcttt tacggtttac gacagtcaaa
    29401 tacactcgat caaaagttcg aaaaggattg ccgaaagaat atagctatat cattgaggaa
    29461 ctcttgtata tcgatgatcg tatcaacggg aaaaaagact atgtaaaaaa aatcattgaa
    29521 caacttgtct tgatgaagga agaagaaaaa tttttaacaa aacttgcgca gacgatccaa
    29581 aaatctgtca tcgatcattt gcatatcgtg ggtgatattt ttgatcgagg aactcaagca
    29641 gccaaagtaa tggatcgaat catggatttt tcctctgtag atatccagtg ggggaatcat
    29701 gatattttgt ggattggtgc gtattgcggc tctgaggcat gtttattgac gcttttacgg
    29761 attgccgcac gctacaacta tttatttgaa ttagagaaag aatatggatt aaatttacgt
    29821 ccattatttt cttttgccca tcaaacctat cagaagaatc cagtatttac ccctaaaaac
    29881 aggaaagatc tatctgaaag agagcaagaa gaattagaaa agatccatca agcactttct
    29941 atcctccaat tcaaatcaga acatcagttg ttgaatcgac gacctgaatt taaaatggac
    30001 gatcgtaaat tgttggaaaa aatcaactat gaggaatcaa cgatcgtttt agaaggtcag
    30061 ctctatgagt tgaaagggac ttgttttcaa acgatccagc cagatgatcc taaaaaattc
    30121 ttggaagagg aacaaaaagt gatcgatagt ttgatgtatt cattccagca ctcgttaaga
    30181 atgcaaaaac acatgacgtt tttgatcgaa aaagggggaa tgtatctcac gaacaatcag
    30241 catgtactgt tccatggctg tatacctgta gatgaaaagg ggcagctctt agcatttgaa
    30301 ttagaccaat cttatcgcgg aaaagaactg ctgggatttt ttgaaaaaca gataactgaa
    30361 agcgcaaaag atttgactgc aaatgaagat ttggcgaccg atttgatttg gtatgcgtgg
    30421 agcgggccat tttcgccttt attcggcaag agtaaaatgg caacttttga gcgctatttt
    30481 ctcacagagc ctcatacgca cgtcgaaaaa agcaatccct attatcactt gcgagatgaa
    30541 gagtggttca gtgaaaaaat tttggcagaa ttcgatgcaa aagaagaagg ttcagcaatc
    30601 atcaatggac acacgccagt aaaagtaaaa aaaggagagt caccgatcaa agcaaacgga
    30661 aaagcatttg ttattgacgg aggtctatcc aaagcttatc aaaaaacaac cgggattgct
    30721 ggctactcat tactatgcaa ttcttacggt ttccaaatcg tgacacatta tccgttcttg
    30781 cctattgaac aacaatttgc aaaaaaatct gatcaaacga atgtaaaaaa agtgatcgag
    30841 cagcagctcc cacgcaaact taatcggaat acgacaaaag gaatggaatt acagcaacag
    30901 attactcaac tccaaaggct gttggattat cgaaaggacg attgagaaaa atgctagcca
    30961 acattgctca atagactaaa aagcgactgc aacaaaaggc gtgtcgcagt ctccttcgga
    31021 attagctcaa aaaacggtaa aatattagaa gctatttttc tgtttatttc tctaatactc
    31081 aggtcagggc gctgatttca cgacctcttc cttaaacggt gttctcctgc caactcctcg
    31141 gtcacaagtc acaatatcca acaaacatga gaagctgttt tttgaaattg ctccttgtga
    31201 ctcagagttg aacggcaggc tcacgaccta atcagaaaaa cgattaaaat tcattcacaa
    31261 agaaatcgaa taaacgctgt tcagatgaat ctacacgatg agaaagttcc ggatgccatt
    31321 ggacaccaag gattttgtgc atattatctt tactttggac tgcttcaatg agtccatcgt
    31381 tggaaaaggc gatggctttt aacaaaggag aaagatcttt gactgcctga tgatgatagg
    31441 aattcacaaa gtatgaatca gggagaaggt cacttaataa actgtccgaa acgattttga
    31501 cttcgtgagt tgcaaactgc ggggcagtgg gctgttgttc gtgttttacg gaccattttg
    31561 gatataatga aagatcttga taaagtgttc cctccaacac gacattcaat agttgaagtc
    31621 ctcggcagac tgcaaaaatc ggttttcttt gtttcaatgc ttccaagatc aatgcctgtt
    31681 cgaattggtc tcgttgaata tttgtttccg tcagttttgg atgagggtct tccatgtaaa
    31741 gttgcggact aacatcctga ccgcctgcaa gaatgagttt gtcgattttc tcaattagtt
    31801 gaggcgctga atctggagac atgactggca gaataagagg taatccttga gcatctaaaa
    31861 ctgcatccac aaatctctgc ggtgtatagc ttacttgatt tccttggaaa gtatctgtcg
    31921 ctcgaatcag ttgattcccg gcaatgccga tgattggttt catttttatt ccccctaaag
    31981 tagactaatt gtaacaagga tttaaaaaaa tttaaactat gtaagacttg cccctatact
    32041 tatctgttct tgctgtgtta ctatctgtaa agagaatttt cgtgtgaaat tcatgacgat
    32101 agttgaaaat attgcttaat ttgttataat aaaaagtaag cagaaataaa aaggttgggc
    32161 gtaagcttca aggatctagt tcttcaaggg gtgagaagga cattttattg aaggggcctg
    32221 cctatactaa gttatcttta gaggtacata ctttcaatga aaaaagaaag catgtacctc
    32281 ttttttaaat cgaaaggaag tgaagcaagt gttagaagct ttggaacaaa tccgaaaagc
    32341 agaagaagaa aatgagaagc gtgctcgaac cctacggcag cagttgaaga cctatgcaga
    32401 agaaaaagag caagaattgg agataatcca aacgaacagt cggcaacagc tgaaacagtt
    32461 ggtagaagaa aaagaacaat tcgaaaagca agaattagaa aaagaaaaaa tgaaattgaa
    32521 agcagaagca gacaaggtgc aagcaacttt gaaagaccaa tatcaaaagc atcagagtca
    32581 agcaatcggt gcaatcattg aaagggtgaa agaaacttat ggccgtcact aagatggaaa
    32641 aagtgaccct catttcagat aaaagaaatc aggagaggat tttacaagca gtacaaggaa
    32701 tccaaaatgt tgaaatccgg gatctgtatc aaaatagaac aaacaaccag tgggtagaaa
    32761 aatattttcc agataccacg attattgata aagaagcaaa attaagcgct ttaggcaatc
    32821 ggctgaaaga catccaagaa gctatccaat tcatacaaca ccacggcgat agccgtcaaa
    32881 agaaactcca tttgaaacgt caagaacttt ccttacaaga cttggaagca aattattccg
    32941 aagaaaattt catgaataaa ttagaagaag tccttgattt aaaagaaaag tgggaacagc
    33001 taactgaaaa acgtgatcgt ttagaagaag aggaagaatg gcttcttcat tggcaacagc
    33061 tggatattgc tccaagtatg tatgcaagta agacgaccat ttttgagatg gggactgtga
    33121 gtgcagcaaa ttttgaaagt ttcaaggaag aactaagtaa gcttgatgac gtttatatgg
    33181 aagaaataga ttatacgcca aaacaagttt atctttcgta tgttgcttta aaaaaatcag
    33241 ttgctgctat agaagaaatt gctgggaaat acgggttcaa taaagaaacg tatccttatg
    33301 aacaaaatcc taaagaaaaa ttgcaagaaa ccaaacagca attatccatt gttcacgagc
    33361 agcaaaaaaa gataacgaca gcgttaggtc agtgcagcgg ctatatccaa gaatttgaat
    33421 gggtagaaga agtcacactt gccgttgcag aacgggaaaa agtaaaagaa cggtttatcc
    33481 atgccactta tctgatcgtt ttgcaaggtt gggtagatgc agaagaaaaa cagaatttga
    33541 tgcatgtgtt aaacgaaact gtatcagaag aagatatata cgttagtttc gaaacaccaa
    33601 actcagaaga gattcagcag gaagtcccta ctaaattgaa aaaccatccg cttatcgaac
    33661 cttttgaatt gctgactgaa atgtacagtc tgccaaaata tgaagaagta gatccaacgc
    33721 cttggatgac accattttat cttgtcttct tcgggatgat ggtcgcagat gttggttacg
    33781 gattgctgat gctcattggg acaatactag ctcaaaaact atttgtttta ccccgcggga
    33841 tgaaacgttt cgtaaaattc tttgagattc tctcggtacc atcgatcatc tggggattga
    33901 tctacagttc atttttcgga caggcattac caaaagaact atttggtatc cgcttgcctt
    33961 tcccaatcct atctacgacg gatgacgtca atacgatttt gattttatcc gtgattttcg
    34021 gtctgataca gatcttggtt ggattattcg tggcagcaaa ggaaaatatc aggaggaaag
    34081 attacttgag tgctgtcagt gatggttttg cctggcaagg gatcctgatt gggatcgtta
    34141 ttgcattgat tggtgtcatg ttgataaaaa atacagcatt tgtttatctt ggcgcggggc
    34201 tatcgatcat tgctgcttta tgtatcttaa tcgtgccgat tatccaatcg ccgtctaaag
    34261 tcaagggtgc tgccaaagga gcgtataatc tttacggatt gactggttat atcggtgatt
    34321 tagtcagtta tacacgtttg atggcactag gtatttcagg cggaagtatt ggcgcggcat
    34381 tcaatatgct agttgctttc atgccgccag ccgctcgatt cagtgtaggg atccttttga
    34441 tcatcgcttt acacgcactg aatatgtttt tgacactgtt aagtgcctat gtccatggtg
    34501 cacgattgca gtatgtcgaa ttttttggaa aattttatac aggaggcgga cgtgcctttg
    34561 acccgctgaa aacggcggaa aaatacgtca acatcaatca taagaaaaaa aacaataaat
    34621 aattggagga tttttataca atgatggatt acttaattag tcaaaatggt ggaatggtat
    34681 ttgcagtatt agcgatggca acagcgacga ttttttcagg gattggttca gctaaaggtg
    34741 ttggaatgac aggtgaggca gcagcagcgt taacaacaag tcaacccgaa aaattcggac
    34801 aggctttgat tctgcaattg cttccaggta cgcaagggtt gtatggattc gttatcgcct
    34861 ttttgatttt tatcaactta ggaaatgata tgtcagttgt acaaggattg aactttctag
    34921 gcgcttctct gccgattgct tttacaggtt tgttttcagg tatcgcccaa gggaaagttg
    34981 cagcagcagg tatccaaatt ttagcgaaaa aacctgaaca tgctacaaaa ggaatcattt
    35041 ttgctgcgat ggttgaaaca tatgcgattt taggtttcgt tatttccttc ctattagtat
    35101 tgaatgccta agcataaagg aggaaaagca gatgaacgcc attgaaaaaa tcatctctca
    35161 gatgaatgaa gcagcagagc aagaaagagc ggcgttagag caagaagagc gaatgaagat
    35221 cgatcaaaac tttgaacaaa aacgtacgca aatcgaaaca gaacatcaaa aacaaaaaga
    35281 aaaacaaatc gaattacttg aaaaaaaata ccgtcagctt aggaatcgcc aacaagtaga
    35341 agtacgacaa gaaaatttga atgcgaagca ggaattttta cgacgattgt ttgcagatgc
    35401 aatagctgaa atggagaatt gggatgaatc tgaacaaatc cagtttatca aaaattcatt
    35461 gtattcatta cctttgacag gcaaagtaac attcattgct ggagaaaaat cagcagctta
    35521 tctttctcag acattgctgg atgaatggaa caatgaactt ccctttatga tggttttaag
    35581 tgatgaaact gttgctgatc aagcgggttt tctgataaac gatcaaggag ttcagtataa
    35641 ttttcttttc agcagccttg tacaagatat ccaaggaacg atgagttttg agattgccaa
    35701 tcaattattc gaataaggag ggatcagtgt atggaatacc acgaactaaa tcccttgatt
    35761 cgcggaagag agcttgaact tttgtccaat gagacatttg aacggatgat tcaaacaaat
    35821 tctgtcgaag cacttggtga aatattgaaa tccacgattt acagtccata tatttatgac
    35881 ggatatgaat atgacttcga acagaatctt ttaaaggagc agagccgtct atttcgctgg
    35941 ttaaaggaat cagcaccgga gcctgaaatc gtttggatct atacgatgcg ctatactttc
    36001 cataatctga aggttttgac aaaagccgag atgacaaatc aaaatctgga ccatttgtat
    36061 atcgacgatg gattttattc attagaaacc ttgaaggatg ccattcatac gcaagtttca
    36121 aatgaactcc ctgaaagcat tatggacagt atacgagaag tacgtgatta ttttgaagag
    36181 tctaccattc ttcagggaat tgacgtgatt tatgatcgac agtttttgac agagcagcga
    36241 cggcttgccg ataagctagg atatcaagaa ttgctgacag aagtcattgc ttttattgac
    36301 ttgaccaata tcacaacgat ggctcgagga atattgcaga aaagaacata tgggttcatg
    36361 acgacggtac tttccagctc gggaagtatc gaaaaagaag tatttttgaa ctttgtagat
    36421 aaagatatgg aagattacac gaattttcta ttagagacag attatgctga ttttctgcgc
    36481 cctgtcatcc gtgagggaaa tcttgatctc gtaaagcttg aacagttgaa agatgattat
    36541 ctaagttcat tgtatcaaga agcacaaacc caggctttcg gaccactgcc actactagcc
    36601 tttttgaacg caaaagaggt agaaagcaaa aaccttcgtt tactggttat cggcaaaaga
    36661 agccatttcg aaccagaaac aatcagaaag cgggtgagac aagtctatga cgcataagat
    36721 cggtgtagtt ggcgacaaag attctgtttt gccattcaaa ttgtttggct ttgatgttcg
    36781 ttatggtaca accaagcaaa ccgttcgtca ggcaattgaa gaaatggcaa agaatgagta
    36841 tggcgtgatt tatgtcacag agcaatgtgc agcattgagc ccagaaacga tcgaacgtta
    36901 caaggaacaa atgatacctg ctatcgtatt gattcccaat catcaaggat cattagggat
    36961 agggctggaa gaaattcaaa aaaacgtaga aaaagcagta ggtcaaaata tcttatagta
    37021 tgaaaaggag atagaaattt tgcaaattgg aaaaatcata aaagtctccg gacctcttgt
    37081 aatggctgag aacatgtctg atgccagtat ccaagacatg tgtctagtgg gagatctagg
    37141 agttatcgga gaaattatcg agatgcgtgg cgatgtggcg tctattcaag tatatgaaga
    37201 gacttctgga attggtccag gcgaaccggt tcgctctact ggtgaagctt tatctgttga
    37261 attaggacca gggatcattt cacaaatgtt tgacggaatc caacggccat tagatacctt
    37321 tatggaaatc acacaaagta atttcttagg gcgcggagtt caattgcccg cattggacca
    37381 tgagaaaaaa tggtggttcg aaccaacagt agaagccgga gaaacggtga gtgctggaga
    37441 tgttatcggg atcgtagaag aaacaaaagt tataaaacat aaaatcatgg tgccaaatgg
    37501 gataaaagga acgatcaaaa caatcgaaaa aggttcattc actattgatg aaacagtatg
    37561 tgtcattgaa accgaaaatg gtgacaaaaa tttgacgatg cttcaaaaat ggccagtaag
    37621 acgtagtcgg ccaatcaaag aaaaattaaa cccggatgca cccatgatca ctggacagcg
    37681 agtgattgat acattcttcc cagtgacaaa aggaggagca gcagctgtac ctggtccttt
    37741 tggtgcaggg aaaacagtcg ttcagcatca aattgcaaaa tgggccgatg tggacatggt
    37801 tgtctatgtg ggatgtggcg agcgtggaaa tgagatgacg gatgttttaa atgaattccc
    37861 tgaattgatt gaccctaata caggagaatc gttgatggaa cgtacggtat tgattgctaa
    37921 tacatcaaac atgccagtag cagctcgaga agcttctatc tatacgggaa tcacaattgc
    37981 ggaatatttc cgtgacatgg gttatgacgt tgctatcatg gcagactcca cttcacgatg
    38041 ggcagaagca cttcgtgaga tgagcggacg tttggaagaa atgcctggtg atgaaggtta
    38101 tccagcttat cttggttcac ggcttgcgga atactatgaa cgatctggaa gagtcattgc
    38161 attaggatca gaaggtcgcg aaggaagtat aaccgcaatc agtgctgtat ccccgtctgg
    38221 aggggatatt tcggaaccag tcacacaaaa tacattgcgg gtcgtaaaag tattctgggg
    38281 tcttgattcc agtttagccc aaaaaagaca ttttccttcg atcgattgga tacaaagcta
    38341 ttctctttat tcaacagaag tagggaagta tatggatcat atccttcaag ctgactgggc
    38401 atccatggtc accgagggca tgcgtatttt gcaggaagaa cagcagctga atgaaatcgt
    38461 gcgtttggta gggattgatt ccctttcaga taatgaccgt ctgacattag aagtggcaaa
    38521 atccattcgt gaagattatt tgcagcaaaa tgcttttgat gatgtcgata cattcacttc
    38581 aagagagaaa caattcaaaa tgttgaagat gatcttgacc tttggtgaag aaggacgccg
    38641 tgcgttgtcg ttaggtgctt atttaagtga gatcatggac ggtaccgtat cgattcgtga
    38701 aaggatcgga cgaagcaaat atatcccgga aacagaatta gaaaaactag atcaaattat
    38761 tggtgaaatc aagcaaacga tgcaaacgat cgtttcagaa ggaggcatga gcaatgatta
    38821 aagaatatcg tacgatcggt gaagtcgtcg gtccattgat ggcggttgaa aaagttgctg
    38881 gtgtcaaata tgaagaatta atcgaagtcc gtatgcaaaa tggcgagatt cgtcgtggac
    38941 aagtattgga agtccaagaa gataaagcaa tggtccaaat attcgaagga acaagtggta
    39001 tcaatttacg tgactcttct gtacgatttt taggccatcc tttagaacta ggtgtatcgg
    39061 aagacatgat cggtcgagta ttcgatggtt tgggacgacc gaaagataac ggaccagaga
    39121 tcctgcctga aaagcatttg gatattaacg gagaagtaat caatccagta gctcgggatt
    39181 atccagatga gtttatccaa acaggtatct ctgcaatcga tcatttgaat acattagttc
    39241 gtgggcaaaa attaccagta ttttctggct ctggtcttcc tcataaagaa ttagctgctc
    39301 agatcgcgcg acaagcaacg gtactcgatt catcagatga tttcgccgta gtctttgctg
    39361 ctattggaat cacttttgaa gaagctgaat ttttcatgga agattttcgt caaacgggcg
    39421 caatcgaccg ttctgtcatg tttatgaatt tagcaaacga ccctgcaatc gaacgtatcg
    39481 ctactcctcg tatggctttg actgctgcag aatatcttgc ttatgaaaag ggaatgcatg
    39541 ttttggttat catgacagac atgaccaact atgcagaggc attgcgagag atttctgcag
    39601 cacggcgaga agttccagga agaagaggat atcctgggta tctttacacg aacttagcta
    39661 ctttattcga acgagcagga agaatccgcg gactaaaagg atcggttact caaattccga
    39721 ttctaacaat gccagaagac gataaaactc atccaatccc tgacttgact ggttatatta
    39781 cagaaggaca gatcatcttg actagagagc tttacaagag tgggattcag ccaccaattg
    39841 atgtactccc gtctctttcg cggttaaaag ataagggaac tggacccgga aagacaagag
    39901 aagaccatgc agctacgatg aatcagcttt ttgcagccta tgcacaagga aaacaggcaa
    39961 aagaattagc cgtagtttta ggtgaatctg ctttatctga tgtagacaag atctatgcga
    40021 agtttgctga gcggtttgaa aaagaatatg tcaatcaagg attctacacg aaccgttcga
    40081 ttactgaaac acttgatcta gggtgggaac tgctatcgat gttgccaaga accgaattaa
    40141 aacgaatcaa agacgacatg ctggacaaat atctgccaga agggaagtga gttctgatgg
    40201 cacgattgaa tgtgaatccg acccgcatgg aattgactcg attgaaaaaa cagttgacaa
    40261 ctgccacaag agggcataaa ttattaaaag ataagcaaga tgaactgatg cgtcaattca
    40321 tcctgttgat tcgaaagaac aaccagctgc gtcaagaaat ggaagagtct atgacagaag
    40381 caatgtctga ttttgtattg gcaaatgctt cgatcacgga acctttcatc gaagaattat
    40441 ttgttttgcc ggcggaaaac gttgatttgt ctatcgtaga caaaaatatc atgagtgtaa
    40501 aagtacctgt gatgaatttt gaatatgatg aacagctgac agacacacct ttggaatacg
    40561 ggtatttgaa ttccagtgcc gaactggatc ggtcaatcga tcggtttact accctgcttc
    40621 caaaactttt ggaactgaca gaaatcgaaa aaacttgtca actgatggcg agtgaaatcg
    40681 aaaagacacg tcggcgagtc aatgcattag aatatatgac gatccctcaa ttagaagaaa
    40741 cgatctatta tattcgtatg aagctggaag aaaacgagcg cgcagaagta acaaggttga
    40801 tcaaagtaaa aaatatgggt accactgact aagtggtatc tttttttaaa agaggaaaac
    40861 gatgattaaa caaagcaaac aaataaagaa acgtttgccg gctgttcagc ttctcgcatt
    40921 aggatttttt ctcctgattt tagttggcgg cggcctactt accctaccat tttttagtaa
    40981 tagtgggcat gggacaaatt ttatcgatgc gcttttcact gcaacttctg ctgtctgtgt
    41041 aacgggattg acaacattga atacagcaga gcattggaac agtgctggtc aatttttgat
    41101 catgcttttg attgagatcg gcggtcttgg ttttatgatg gttccgattc tatgctttgc
    41161 attgataaag aagaaagtca gtctcagcac acgtattata ttaaaagaag cgctgaatct
    41221 agaagaagta tcaggtgtga tgaagctcat gctttacatc ctaaaattgg cctttgttat
    41281 ccaattgatt ggtacggttg ctttatcttt tgtatttgtg cctgaatttg gctggttgaa
    41341 aggtatctgg tacagtttgt tccatgcaat ctctagtttt tgtaatgcag ggtttgattt
    41401 gttaggagac agtttagcca atcaccagca aaatgtttat ttgatcatgg tggtatctgc
    41461 tttgatcatt tctggtgggt taggcttcat cgtatggcgt gatttactgg aatatcatca
    41521 gaaaaagaag atgacgattc attcaaaaat cgcattaacc gttacaggta cccttttatt
    41581 aggcggattc gttgtgttct ttttcacaga gcgcaatggg gcaacgctag tggaagggaa
    41641 tatgtttcaa cgattggcaa acaccttttt tatgagtgtg acacctagaa cggctggata
    41701 ttattcagtt gattacctcc atatgagcca tgcaggtctg attttaacga tgtttttgat
    41761 gtacatcggc ggaacatctg ggtcgacggc cggcggattg aaaacaacta cgttaggtgt
    41821 gttgctgatc cagatgcgtt cgatgttgaa aggacgtacg agagcggaag catttggccg
    41881 taccatccga caaggcgcag tgttgcgggc acttacttta ttttttgtta cgttgacgtt
    41941 gtgtgtagtc gccatcatga ttttatcggt gactgaaacg atacctaaaa cttctggcat
    42001 cgaatatatt gcttttgagg tcttctctgc gtttggtaca gtaggtctta cgatgggatt
    42061 gacgccagac ttgactgcaa tagggaaatt gatcattata gcgcttatgt acattggacg
    42121 agttggtgta ttgactgtgg tcttctctct gatgacaaaa gcacaaagaa cacaatcaaa
    42181 atacaaatat ccagaagaaa gtgtcatgat tggataattt catcttctga cagaaaccta
    42241 gtgatttagt aaaataagaa aataaaataa tttttcttgc ttcttggata ctttaagtgc
    42301 aaaatgttcg tttatttttt gaggcactac ccgttgttga acctcgggta gtgcctcttt
    42361 tttattgaaa ggaa
//
LOCUS       DAYYCY010000007        37214 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000007 DAYYCY010000000
VERSION     DAYYCY010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37214
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.007"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..808
                     /locus_tag="ACHAZB_000295"
     CDS             <1..808
                     /locus_tag="ACHAZB_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFM8613007.1"
                     /translation="GYELDATPIEFEIERSQTAVVELTKENRLTPGGVVLTKIDDQSG
                     EVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGVYQLVETQAPTGYELDATPI
                     EFEIERSQTAVVELTKENRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLT
                     TGADGKLAIDGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVAD
                     SSDIRGNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKRNI"
     gene            complement(1161..2009)
                     /locus_tag="ACHAZB_000296"
                     /pseudo
     CDS             complement(1161..2009)
                     /locus_tag="ACHAZB_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2162..3274
                     /locus_tag="ACHAZB_000297"
     CDS             2162..3274
                     /locus_tag="ACHAZB_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFM8613008.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(3352..3645)
                     /locus_tag="ACHAZB_000298"
     CDS             complement(3352..3645)
                     /locus_tag="ACHAZB_000298"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HFM8613009.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            3813..4127
                     /locus_tag="ACHAZB_000299"
     CDS             3813..4127
                     /locus_tag="ACHAZB_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HFM8613010.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            4148..6127
                     /locus_tag="ACHAZB_000300"
     CDS             4148..6127
                     /locus_tag="ACHAZB_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM8613011.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            6140..6466
                     /locus_tag="ACHAZB_000301"
     CDS             6140..6466
                     /locus_tag="ACHAZB_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HFM8613012.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            6482..7000
                     /locus_tag="ACHAZB_000302"
     CDS             6482..7000
                     /locus_tag="ACHAZB_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8613013.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            7000..8352
                     /gene="celB"
                     /locus_tag="ACHAZB_000303"
     CDS             7000..8352
                     /gene="celB"
                     /locus_tag="ACHAZB_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFM8613014.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(8436..9941)
                     /locus_tag="ACHAZB_000304"
     CDS             complement(8436..9941)
                     /locus_tag="ACHAZB_000304"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HFM8613015.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(10033..10623)
                     /locus_tag="ACHAZB_000305"
     CDS             complement(10033..10623)
                     /locus_tag="ACHAZB_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM8613016.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            10802..12448
                     /locus_tag="ACHAZB_000306"
     CDS             10802..12448
                     /locus_tag="ACHAZB_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HFM8613017.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            12497..13216
                     /locus_tag="ACHAZB_000307"
     CDS             12497..13216
                     /locus_tag="ACHAZB_000307"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFM8613018.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            13695..14642
                     /locus_tag="ACHAZB_000308"
     CDS             13695..14642
                     /locus_tag="ACHAZB_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8613019.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            14654..15574
                     /locus_tag="ACHAZB_000309"
     CDS             14654..15574
                     /locus_tag="ACHAZB_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8613020.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            15615..17072
                     /locus_tag="ACHAZB_000310"
     CDS             15615..17072
                     /locus_tag="ACHAZB_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8613021.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            17417..18031
                     /locus_tag="ACHAZB_000311"
     CDS             17417..18031
                     /locus_tag="ACHAZB_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFM8613022.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            18024..19748
                     /locus_tag="ACHAZB_000312"
     CDS             18024..19748
                     /locus_tag="ACHAZB_000312"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM8613023.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELRELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            19745..21196
                     /locus_tag="ACHAZB_000313"
     CDS             19745..21196
                     /locus_tag="ACHAZB_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM8613024.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(21669..23822)
                     /locus_tag="ACHAZB_000314"
     CDS             complement(21669..23822)
                     /locus_tag="ACHAZB_000314"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HFM8613025.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDREYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(23824..24870)
                     /locus_tag="ACHAZB_000315"
     CDS             complement(23824..24870)
                     /locus_tag="ACHAZB_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8613026.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            25020..26309
                     /locus_tag="ACHAZB_000316"
     CDS             25020..26309
                     /locus_tag="ACHAZB_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HFM8613027.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            26322..29024
                     /locus_tag="ACHAZB_000317"
     CDS             26322..29024
                     /locus_tag="ACHAZB_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFM8613028.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            29009..29866
                     /locus_tag="ACHAZB_000318"
     CDS             29009..29866
                     /locus_tag="ACHAZB_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFM8613029.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(29937..30794)
                     /locus_tag="ACHAZB_000319"
     CDS             complement(29937..30794)
                     /locus_tag="ACHAZB_000319"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFM8613030.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            31005..33176
                     /locus_tag="ACHAZB_000320"
     CDS             31005..33176
                     /locus_tag="ACHAZB_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFM8613031.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPAVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            33244..33585
                     /locus_tag="ACHAZB_000321"
     CDS             33244..33585
                     /locus_tag="ACHAZB_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFM8613032.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(33861..35615)
                     /locus_tag="ACHAZB_000322"
     CDS             complement(33861..35615)
                     /locus_tag="ACHAZB_000322"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8613033.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQCISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(35626..36462)
                     /locus_tag="ACHAZB_000323"
     CDS             complement(35626..36462)
                     /locus_tag="ACHAZB_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFM8613034.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            complement(36464..37147)
                     /locus_tag="ACHAZB_000324"
     CDS             complement(36464..37147)
                     /locus_tag="ACHAZB_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HFM8613035.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLMPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
BASE COUNT        12005 a         6542 c         7633 g        11034 t
ORIGIN      
        1 ggggtatgaa ttagatgcga caccgatcga gtttgagatc gaaagaagtc agacagcggt
       61 ggtagaattg acgaaagaaa accgacttac accaggcggt gtcgtgttga caaagatcga
      121 tgaccaaagc ggggaagtcc tgcaaggagc tgtttttgag ttacaagatg ccaatggaac
      181 cgttctgcag tcaggtctaa ccacaggagc agatggaaaa ctggcgattg acggcttagc
      241 accaggagtg tatcagctgg tagaaaccca agcgccaacg gggtatgaat tggatgcgac
      301 accaatcgag tttgagatcg aaagaagtca gacagcggtg gtagaattga cgaaagaaaa
      361 tcgacttaca ccaggcggtg tcgtgttgac aaagatcgat gaccaaagcg gagaagtcct
      421 gcaaggagct gtttttgagt tacaagatgc caatggaacc gttctgcagt caggtctaac
      481 cacaggagca gatggaaaac tggcgataga tggcttagca ccaggagcat atcagctggt
      541 agaaactcaa gcgccaacgg ggtatgaatt ggatgcgaca ccagtaactt tcaagatcga
      601 aaaagaacaa gaagtggcga tctttttaac aaaagagaat agaaaagttg ctgatagctc
      661 agatataaga ggaaacaatc caacaagcag tggaaataag catcttccaa aaactggaga
      721 aaccatcatt agccagttta tattgtcaat attgggtgtg ctacttgttt tcatcagtat
      781 aaaatttaga aagaaacgaa atatatgagc taaaataaaa aattgaataa gcatagtttt
      841 taaaatcaac tagtacccat caattgaatg gaaactaccg attttagaag gggtatacaa
      901 acaaatatta gtatcggagt agagaaggtt gaacccaacc agtaaataag taaagacaat
      961 tttgtgctgc gttgagaagt gaaaaataaa cctttgtagt aatactagtt aataaaaaat
     1021 atacaaaaac actgcgaaaa tcattcttga gtttcgcagt gttttttgcc taatgactca
     1081 agtttattgg gtaatacttt gattttcttt ctacaaataa tcagcgatca gttgtaattt
     1141 tttctctagt tttaatggct ctttgtcaat aaggagaaaa acaagtcggg tcttttgtct
     1201 cggaaagctt caaccaactc gtgaataaag gttacgcctc cttgaaaggc gtggaaaaac
     1261 caagtaatcg atcaatcacc aatttcgcat ttggtagaac atgtttggtg agctgaaagt
     1321 aggtggcgtt catgtctgta actaagaatt ctatttcttt aggattagtg cagctaagga
     1381 aatagtttgt taatcgagga attttttgtg taggtaaatt ttctcgatta atttttctgt
     1441 ctcgccatcc gcggaaataa aactcagttt atcctcaacg gaaacgtgag aacgaaattc
     1501 acaaccctca acactcaaga cagaattctt ttggaagact ttggtaaata gcttctcaac
     1561 tctttcaatg tgcgaataac ggttgtcaaa gatacctaac aacttttcga aataaaaaat
     1621 aacgacactt tttcagtcag taaagaagtg attttatgtc tgacatgatt tgaaatcgag
     1681 tgccttggac ggataaaata actttgagcg ttccaatggc ttcgacagtt tttacaggta
     1741 tagcgctgct tttttaggcg tataaccatt ggcatatgat tatattattc aaagtggaca
     1801 atggtttcct tcttcttgtt tttcaccacc attaatttcc tatttccatc taccacagca
     1861 gaaccacaat ttctacagac acatggagca gggaagagaa ctgcagcaac gatcaatgtt
     1921 tttttcttct gtaaggtttc gtaagagacc tctgtaatca tcaaattttt gtctattgat
     1981 ctcagaattt ttttgataga accattcata aagcatatcg ttctctcagt tgtttatttt
     2041 gtggtgattt aatcatacaa gaaaacgata tgttttttta taaaaacatg aataggaaaa
     2101 atagttgaca agaaaatgac aacgccatat gatgttccat agtatttaca acctgtcata
     2161 gatgactgta aaaaataaaa gtaaaaagaa aggacgacaa caggtggatt tttatcagtc
     2221 tctacaacta gatccgttta ttttaaaaca aaaaatacga gaagcagctt ctaaaaaaga
     2281 aaagtgcatg tatatttgtt cattgttttt acgatcatta ttcatcgtat tgtttgccat
     2341 ttgttttatc attataatta caacattatt tgaatcgaag cataaaccat acgctgtggt
     2401 tttattctgt atgttgatga gtattcgttt tgttgacttt ggttataaga tttcccattc
     2461 gatcataggt ttagcaatcg tcatgttctc actattagta gcaccgtatg tccagctgat
     2521 caaatggtca gttatgggca tgttgattca ttttatttta ctaagcagca tattaatggc
     2581 aaccgctagt gatcccaaaa tggggaatgc ttctttgtac ggcttttcat atctctttat
     2641 tgtttattcg ttacctaaag attcgttgaa caagaatttc tttacacaaa caagtagctt
     2701 attatttttg tttttttgtt ggttttcagt tctcctttac agaaaacatc gagaaaagaa
     2761 caaagacaaa agcttgttta aagagatttt tttgaaaggc atgtattcgc aggaaaaaat
     2821 ttggatgctc atctatgcat ttggcattag tcttttgatc gttgcaggag agtatgtccc
     2881 ttttcagcga ttgatgtggg cagggtttgc attttcttct attgtatcta gctatggatt
     2941 gatgtcgatt ggtttcaaag aacgagcagt agaccgaatc attggctcat tgattggatg
     3001 tgttttattt attggtatca gccaatttat tccttttaac tgggtcggta tattaggtgg
     3061 tctagcttta ggtgtttgtt ctacctatag atataaaaca gtttttaatt cttttggtgc
     3121 tttggcaatc actgccagtt tattcggggt tccaggagca gtaacgatcc gcatttttga
     3181 gaatatcctt ggggtatgtt taggaatcat gtatattggg gtgactgaaa tacttatccg
     3241 aaaaataaga gaaaaacacg gtttgaatca ttagatgaga tcgaataaaa aataaggaaa
     3301 aagtgaagat acacatggat gataaattgc tatcattcca tgtgtatctt tttacggtaa
     3361 atcagtgaaa cgttcgactt tcatttttaa tttatacttt ttacggagtt cgtttatttg
     3421 agccatcatt tcggatttgt gatgcttttc gattgcttct tcatctttcc aacggtcgat
     3481 cagtaaaaga gattctggat cttctagtgg aaagtaatag tcatatttct cgtttccttc
     3541 ttctgcacga atcgctttga caatgtttct ttcgaccatt tcttctgcaa attttctggc
     3601 ttgtccattt gtacttgtat acaaaatatt cactgtgata ctcactaaat cccctcctga
     3661 aaaaattcta tcactagtat atcacggtgc cagaaattat tattcaagat atgatacaca
     3721 ttttttccgc aatgctacgg aaatatatat tttgaataga aaacgcttac aagatatgct
     3781 tggtttataa aaataaaagg agtgattgat ttatgaaaaa agcattgatt atttgtgcgg
     3841 caggaatgtc atcctcgatg atggcctcaa agacaacgga tttctttaag caaaaaagag
     3901 aagaggtact cgtagatgca gtttcagcta cagaaggcga taatatgatt aagaatagtg
     3961 attttgattt atttctgatc agcccgcaaa cgacgatgtt tttggataaa ttcaaaaaat
     4021 taggagaatc tgttggaaag cctgttgtta gtatcccttt ccaagcgtat gtcccaatcc
     4081 ctacaggcat ccagaagtta gcagaggtga tcgaagagaa catatgatgg aataggagtg
     4141 gatgataatg aaacagaagg aaaaattgct gttgagatat ttgattgatc acaagaagga
     4201 gtatgtcacc agtcaacgtt tagcttctga attgttatta tctgatcgga caatccgtaa
     4261 ttacttgcag cggatcaaag agttagtaga aaaaaacgga ggtaagatca ttgctaaacc
     4321 aggttatggc taccaactgc atattttaca aagactgaca tttgacttgt ttttgagtcg
     4381 gcaagaaata gtacctggat atgctagaga accgcaagga ttttatgagt ccgaagatcg
     4441 acaaaagtat atattgaacc aattgctttt ggaagatgcc attcttttta tggatgattt
     4501 ggcagaagag ctttatatca gtcgttccag tttgacaaag gacatgcaag aaatcaagga
     4561 gcgattgatt ccatattcac taaaaatcgt ttctaagcat ggtcaaggga catggatcga
     4621 aggggaagaa agaaatcgac ggcattttat catggatact ttttttggaa accattatgg
     4681 aaattcattg aaagaatatt tagggcaaag ccgttttttc ccagacatca gttttgaaga
     4741 gctagtcatt atcattctcg atgaaacaag agaagctaag ttaaaagttt cggattttat
     4801 catacagaat ctgactttac acttggcttt ggggatcaaa cggctccgag aagggtttga
     4861 aatcaaggaa ttaggcatcg aacaagaagt ttctaagcga gtagagtatc aagtggcgca
     4921 gcggatcgtt caacgtattg aggtaatcgc aggtgtatcg tttcccgaag aagaaattgc
     4981 ttatttgaca ttacatctca tggctaagtc gaatcacaaa gaaaatcaaa atgatcagga
     5041 actatcaaag gaattagcga aagttctgaa aagattgtct caggaatttc atttttcatt
     5101 agtagaggat ctccagcttt tcaatggact actcgatcat ttgtcaccga tgatgattcg
     5161 attgagccga ggtattcgca ttgagaaccc attgactgag gagataaaga aagaaaatcc
     5221 caaagtcttt gacgcagtaa aaaggcattt cagcaacatg ccggctttga aaaactatac
     5281 catcaatgaa gatgaatggg cctatttggc tttgcatctc atggcggcat tggaaaaaga
     5341 aagagctgcg cataaactac atgcattgat tatctgtgca acgggttacg gcagtgccca
     5401 actattaaag aatcgcgtag tcagtgaatt cggtaaaaat attacagtgg taagtgtaaa
     5461 aggctattat gagatcaatg aaacaacttt aaaaggaatc gacctgatta tttcttctat
     5521 tgatctttcg acaatgtttt tcaaaattcc agttttacat gtcagtattt ttttgaatga
     5581 agatgatgtt cgtaaaatac gaaaggtagt tggagaatct ataccctatc attcctcaga
     5641 tagaccgata ccagcatttt ctctacagca aaaaaagcag atttacaccg aacagttgtc
     5701 tgaaaaattt ttcaagactt atacgtacga accgaaaaag gaagaaattc ttcatgattt
     5761 gctgcaaaag ctgagcataa atgaagaaga aggatatata agagaaatgc gaaagcagat
     5821 ccagcaacgt gaaaatatgg gacaaatcat attcagtgat acagtggcag tcccacatcc
     5881 cgctgttcca gttggaatcg aaacaaaggt agccgttgca ttgattcctt ctgggatgca
     5941 gtgggatcaa tatcaagaaa ttcattttgt ttttttagtt tcgccttctt atgtcgaaaa
     6001 tgaaggaatt acggtcgtga ctaaagcaat cgttaggtta gttgaccagc taaaagtaca
     6061 gcaggaaatc ttagctgaac ctatttttga gaatttcagc agtaaattta tcaaactgat
     6121 cgaataagga ggaaaacaga tggctgaaaa aataaacagt gaaacactac aagtgacttc
     6181 ttttgatatc attttccata gcggaaatgc acggacactg atacatgaag catttcgctc
     6241 gatgcgtaaa gagatgtttg aagaagtaga agagaagttg cgacaagcaa atgaagagat
     6301 tcttcatgcg catcagtcac aaactcaact tctgaaagaa tatgccagcg ggacaaaaat
     6361 agaaatggaa attatcatgg tccatgcaca agatcatctg atgacgacga tgacattatt
     6421 ggaagttgca caggaaatga attatttgta tcaaaaaaat aaatagaaag gaaggatcat
     6481 tgtgcaggat acagtaaaaa ggaaaatcga attagaaaaa aagcaactta gtttgaaaaa
     6541 catgtatttc aatcgttatc taatcgtacg ttacctgact gcctttttct ttttcatgaa
     6601 catgcaatgg atgattctgc ttctatcagc taaaagttta ggaagtagtt taccgatgat
     6661 tctgatttta gctattttgc ctgcagtagg agaacaagta aagctttatc gaaaacacca
     6721 aacgaacgtt ccatggacaa agtggtattt tttgttccag agtgtcttta atttattagt
     6781 gattccagtg ttgtttacct caggatttac attcttgtat ccttttatgg caaataacaa
     6841 tggaggacag ttatttgttt tcattcttgt tgtaagcggt atttttgcca gtgcatttat
     6901 acagtatcgt ttaaagaaaa tttcactcaa ccaagaccaa cagtatatcc gtattaaaca
     6961 atatgaaaaa gcgttatatt taggaaagga gaataactaa tgtttgattt tttgcagaaa
     7021 tacctgatgg gacctatggg gaaaattgcg caacttaaaa tcgttcgggc tgtcatggca
     7081 gcggggatgg cttctattcc tttcacgatt gtaggctcga tgtttttggt tttaaatatt
     7141 ttgccattac cgttcccatt tttagaagga ttttttaatg caactttttt caaagttagt
     7201 gacttgtaca tgatcgtcaa tacaatgacg atggggattt tatctattta ctttgccatt
     7261 gtgtttgctt atgaattaac gtcaattgaa cgagatgaac aaaaattaaa tgttaatccg
     7321 ctcacggggg cattactatc tgtttttgca ttctttatgt gtatccctga attgatcatg
     7381 actgatggta aaatcagctt gattgcttct atcaatgaga cagaaactgt agtaaatggt
     7441 attaggatgg gctcttttgt cgaacgttta ggaacttcag gaatctttac agctattatt
     7501 atgtcgatta ttgcggtcga attgtattgt atgtgtgtca agagaaattg ggtcataaaa
     7561 atgccagatg ttgttccgcc aggagtttct cgttctttca ctgctctgat cccaacattt
     7621 gtcattgcgt ttgtggtgat gatcatcaat ggattattag ttgcactagg aacggatatc
     7681 ttcaaaatga ttgctatccc atttgggttt gttacgcagt tgacgaatac atggcttggt
     7741 gtcatggtga tttatttctt gatccatgca ttgtggatcg tagggatcca tggagcgaat
     7801 atcatcactt ctttcttgac cccgatcgta ttagctaata tggcagctaa cgcacaaggc
     7861 gcaaattatc cgttggcagg agaattcaac aattcttatg taaccgttgg tggatcaggt
     7921 gcgactttag gattgatcat ctttattgca ttcctcgcga aatctgatca gctgaaagtg
     7981 ttaggaaaag cctcacttgt accaggatta ttcaatatca atgaaccaat cattttcggg
     8041 atgccaatcg tctacaatcc ctatttagcg gttccgtttt tcttagctcc gatggcttct
     8101 gctacactcg cttattttgc cattcggctg gaaatcgtca aaccgatgct tgcacagatg
     8161 ccgtggcctt caccagtcgg gatcggagca tttgttggta gtggtggtga ttggaaagct
     8221 gctgtgttag cagtcatttg tgcaatcatc gcattcatta tctggttccc atttatcaaa
     8281 ttctatgata ataaattatt aaatgaagaa agagaaaagg cagcagaatt agctagtgaa
     8341 aagttaaaat gatttctaag aaaaaaggaa ctgtgacaaa aaatcacagt cccttttttt
     8401 cttagaaatg ggaaaccact agtatataaa taccattatt cttcaatcaa ccgcttcacg
     8461 attgcataat ccccttcata aggttcatct acaccattga ttttttgata gggatccacg
     8521 ccttttcccg caataacgac tgaatcttgc ggagcgcttt ctgctaaagc gcgtcgaatc
     8581 gcttcaggac gatccatttc ataatgaacg atcaaatctg gattagtaat tgcttcattg
     8641 atttcttccg cgattttttt cggatcttca aatgctgggt catctgccgt caaaaaggcg
     8701 atgtcagctg tttctgatag cacttttcca aaatcatgtc gacgggagat tgctttgttt
     8761 cctgggctgc ctaatacaac aattacacgg ccatctggat gttcattttt ggcaaatttc
     8821 agcaatgttt tcaagcttag ataattatgt gcatagtcca catagacatg cgctccattg
     8881 gattggatca attgatccat tcggcctggt acacgagctt cttttaatcc gtcttgtgca
     8941 tcttgactgg aggcacctgt tagtgctgcg gcaatgatcg cactggctgc attttcttgg
     9001 ttgaatcctc cagccagcaa aatatcataa gcagtatcgc agatagctaa gcgatcttca
     9061 tgagaagata aagtaaaacc gtgagtacct ttatctgaac ggatcacttg ataattgctt
     9121 tcttccgaac gaccataagt gatcgttgga atctggaata gatcggctgt ttccctaagt
     9181 aaatcaaaat agtcactttc atgatttaaa acaactgttt ttgaattcaa gatcaattgt
     9241 cgcttgcaat agaaatagtc atcaaatgtc ggatgttcga tcggactgat atggtcaggc
     9301 gagatattca agaagattcc gacatctaaa gtcaagccgt atacgcgttg tgttttgtaa
     9361 gcttgtgaag aaacttccat cactagatga gtcataccat tttcaactgc ttctgacatc
     9421 atacgatata aatctaatga ctcaggcgtt gtcaaatggg atttgaaata tgttttgcca
     9481 tctaacgttg tattcatagt agaaagcaat gctgtttttt tatttgttgt gtgatccaag
     9541 attgcttttg taaagtacgc agcagttgtt ttaccttttg ttccagtgaa tccgatcact
     9601 ttcaatttat tttgcggata gtcgtaaaaa gccatagcaa gaagcgccat tgctttacgg
     9661 atatccgtta cgatgattcc tactgctgaa ttttcgtatg gttgttctga tacataatat
     9721 tgaataccct gttcgattgc tgaatccaaa tattcttctt tgaaattcat tcctttacag
     9781 aaaaacaaag tagaagcatc agcttttctt gaatcataac ttaactgaag aaattcagct
     9841 ttttctggta catcaagatg ccagcctctt gttgaaacaa attcttttaa tagattttct
     9901 tttaatagta aatgacggat gtcttctaaa gagaagctca ttgattgatc cctcgcttta
     9961 ttcaatttct tggtttatta tagcacactc tttttaatat atcgtttgtt tccgctatga
    10021 caaaatccag ctttattcaa attttcttga cacaggggac aagcggatga gtattttcat
    10081 aaacaatgaa ctgactgtaa tagataggag aacgaatagt gtctccaaca atccaaaaac
    10141 ataataattc aaaaagacga taagactatt ggtaagtaaa agtccttgcg gcattggaag
    10201 ccgaaaaaat ttgtggaaaa taatcggcgg tatcgttata ccgccatttg aggccccgac
    10261 tgtgtacaat gtgtaaaccc caagactgaa caaaagactt cctgaaatca gtgaaaagaa
    10321 atgtgaggag atcatttcca cttttggaac aatctctaaa acaacaggaa aaagcagact
    10381 tcccgctaca gtcttaaaaa atactgattt tcctaatagg acagcggcta gaagcagcat
    10441 cataagattg atgctccaaa cagtaactga gcgttggata gaaaaggccg tttcaactaa
    10501 atgtccgatg gcgcccactc cggcggaagc tatatcgtga gggagcaaga accaattcaa
    10561 tccaactgtg ataaagaaaa gacctaaaaa tataacgggt agttttttta tttcttgtgt
    10621 cattgatatc tcctataaat gttagccata aaaggctgta tatttcttta ctttaccaaa
    10681 aaaacgaaac cgaattcaaa aaaacaattg ttcttataaa agttatataa aatcgattgc
    10741 cagtttctgt tttaccaatt aaaatgatag taatgttttg tagaaagttg gaatctgacc
    10801 gatggataat caaagaaaac cagcattaga tcaattaaca ccgcaagaaa aaatggcacg
    10861 aggctctgct tggatgaccg ccagtaatat catttcaaga ttgctaggcg ctgtctatat
    10921 cattccctgg tatgcttgga tgggcgaaaa tgctaaggct gccaatggat tattcaatat
    10981 gggatacaat atatatgcat tgtttctact gatctctaca gcaggaatac ctgctgcgat
    11041 tgcaaaacaa actgcacgct ataattcatt gaatgaatat ggcacttcac gacgattatt
    11101 tatccgtgca ttacagatga tgggcgggtt aggcctgttg tttgctttgt tcatgtacat
    11161 cgcctctcct tggctagctc atgcttctgg tggcggagaa gaactgattc caacgatgcg
    11221 ttctttgagt atcgctgtat tggtttttcc ttgtatgagt gtgatacgag ggtatttgca
    11281 aggcaatcaa gaaatgatgc cttatgcgct gtcgcagatt gtagaacagg tggcacgtat
    11341 tttttatatg ctattggcta cattcatcat catgaaagtc atggatggtg actatgtgac
    11401 ggcggtcacg caatcaactt tcgcggcatt tatcggtatg ctagcaagta ttcttgtcct
    11461 tttgtatttt ttgaagaaac atcaagcgta tacatcggcg tttattcact acagcgaaaa
    11521 caaaataaat attgcaacga aggaattgtt gttagatacc gttaaagaag cgattccgtt
    11581 tattattgtg ggttcagggg taacgatctt caaattagtc gatcagttca cttttatgaa
    11641 gatcatgtct gcatccaccg agtattccaa tgcacagtta ctggatcttt tttcgatttt
    11701 tagtgccaat ccagataagt tgacgatggt agttattgca ttggcaacat cgatcgcgtc
    11761 tactgggtta ccgttgatta cagaagctgt tacgttgaaa gatcgagtag gtctggcaaa
    11821 attgacaagc ggaaatttac agttgttttc attttttatg tttccggcga catttggtgt
    11881 tatgttactt gcctatccgc taaatacatt attttataca ccggattctc taggaagtaa
    11941 tgtactgatc caagcaagtt ttgtaggact cttcctagga ctatatatgc tagtttccaa
    12001 catgctgcaa ggaatgtttg aaaacaaggc ggcaattaat tatttggtta ttgggtttct
    12061 agtgaaactc cttcttcaat acccagccat tcgtttattt gaagtatacg gccctttatt
    12121 ggcgacaatg atcggatttg ctgtgtcttg taccttgatc ttaaagagaa tccatgaagt
    12181 agcccgcttt aaaccaagtt ttgtctggcg caggacattg ctgatcttta ttcttacctt
    12241 gattatgctt ttggcttcgt ggatcacaaa gatgattttt ggtgcttttt taaacgaaga
    12301 tagcaaattc cagtctctat tgttgattct tgtagtagca ggtatagggg ctttagtgta
    12361 tggttattta gctttgaaaa tccgcttggc agaaagacta ctaggaccag gaatagcgcg
    12421 tttacgacat aaattaaaaa taaaataagt gaatcagaag agctagattg atgctctttt
    12481 gaaatggggg aaacgcatgc gtttagataa gtttttagca gatatgggac ttggcagccg
    12541 caaagaagtc aaaggattaa tcaaaaaagg aacgatcgaa gtcaatggac aagtggtgaa
    12601 aagcgataaa gcgcaagtaa atgaattcga agatgcggtt acttatttag gagaaatcat
    12661 tacgtatcaa aaagattttt actacatttt gcataaacct gcaggagtca tttctgctac
    12721 tcaggataat catgatcaaa ccgtaatcga tttattggaa gaccaggatt atcgggaaga
    12781 tctttttcca gtcggccgat tagataaaga cacagagggc ctcttgattt taacgaatga
    12841 cggggtgttt gcccatcagt tactatcacc taaaaaacat gtggagaaag aatatctagc
    12901 tgaggttagc ggtataatga cagaagaaga tatccgttta tttgcagaag gactgattat
    12961 tgatggcgat gagcagacat tgccggcaaa actccaaatt gatcaagtag atcaagagac
    13021 agaaacttca attatccgtc tgattttgca tgaaggaaag tttcaccaag ttaagcgaat
    13081 ggtcaaagca gtaggaaaag aagtcttata tttaaaaaga atccgtatgg gaaaattttg
    13141 gctgcctaaa gatttgaata aagggcaata tcgacccatg accaaagaag agctagaaca
    13201 agtgagagaa aaataattga atggatagct aaaagaataa gcagagtagt tgtagcgcca
    13261 tctgaaaagc agacttgtcc gtctgacttt cggatggctt ttttgtgaaa tttcggctta
    13321 tttccgtaga agctacttct ggaacaccgt ttgttcggaa taagggactg tgacaagact
    13381 tttgttacag tccccattgt tccttgtgac tcagagttag atagtaagga cacgatctcc
    13441 ctaatactca ggccaaaacg ctgaattcac aacctctgat aaacggtgtt caatcagctg
    13501 accctcggta ttagctcaaa aaacggcaaa atatgaggaa ctattttccc gtttttttct
    13561 ctaatactca ggccaaaacg ctgatttcac aacctctcct atactttgtt tcattttccc
    13621 tatactttgt ttatttattc tcattattga aagcgctata attacgttgc gaatgaatcg
    13681 aaaggggttt ttagatgaaa aaacgaaagg ggttcaatca tttttttatg aatctatggc
    13741 gttataaagc acttgtcctg atggctgtac cgggtatgct ttggatgatt tttttctttt
    13801 acatacctgt aatggcgaat gtcgtagcat tcaaaaattt tcatatttca gcagatgggt
    13861 tcttggctag cttgagggaa agtccttggg tgggttggga aaatttcaaa tttttatttt
    13921 cttcggatca agcatttctc atcactaaaa atacaatttt atataatgta gcatttatcc
    13981 ttttgaatct gctgatctca gtcgtatttg caatcattat gagcgagttg agaaataaac
    14041 gactcgttaa agtttatcaa acgatgtcct tactacctta ttttctatct tgggtcatta
    14101 tcagttactt tgtttacgct tttttaagtc ctgacaaagg aatattcaat cagtggatca
    14161 ctggacatgg aggagaagct gtcaattggt ataatgaacc gaaatattgg ccgtttatct
    14221 tggtgttcat tggtacatgg aaagggattg gttataacag cattatttac tttgcatctg
    14281 ttatgggaat cgatccgact tattatgaag ctgctatggt tgatggagct agcaaatggc
    14341 agcagatcaa acatgtgacg ataccacaac ttattccact aatgacgatt ttaacgattt
    14401 tagccgtagg aaacattttc cgagctgatt ttggcctgtt ttataacatt cctaggaatt
    14461 ccggtgcatt atatgatgtg actcaagttt tggatacgta tatctacaat ggcctgacat
    14521 ccacaggaga ttttggtatg acggctgctg caggacttta ccaatcagtt gtcggctttg
    14581 tcttgttgat gatcacaaat acgatcgccc gacgttttga tagcgattct gcattattct
    14641 aaggaggaaa aaaatgaaaa aaaagaaagt ggagagagtc caagtccgct ctttcaataa
    14701 gacggctaat tttttctcta atataatgat tgcactcttt gctatttcct gtattttgcc
    14761 atttatattt gttatcgcca tctcttttac cgatgagtcc atattgtcga ctcacgggta
    14821 cagtttttgg ccagaaaagt tcagtatatt tggttatacg tacttgttcg accagatgca
    14881 ggataaaatt ttccaagcgc ttttcgtcac tgtacttgtt actgttttag gtacactgat
    14941 caatagcacg gctacttctt tatatgctta tgcgatttca cggtcaaact ttccattccg
    15001 tcgttttttt accgtttttt gtttgattac gatgttgttc tcaccaggga tggtggccaa
    15061 ttatcttgtt atgacaaatc ttcttcagtt aaaagatacg atttgggcat tgatcttgcc
    15121 aatggctgtc agtcccttca atattattgt gatgcgcaca ttctttaaac gttcggtctc
    15181 tgattcgatt attgaatcgg cacggatcga tggagctagc gaactgcgaa tatttgttca
    15241 gatcgtttta ccgttagctg ttccaggtat tgcgacaatc agtttatttg ccgcattggg
    15301 ttactggaat gactggttca atgctttact atacatccaa gatgataagc tagttccact
    15361 acagtatctt ttgatgaaaa tccaaagtaa tattcaatat ctgacacaaa attcaggagc
    15421 aggaagccaa ttgacaggcg gattagcctc agttccagga gaatctgcca gaatggcgat
    15481 cgtagtcatc tcgacgttgc ctattgcgat cagctatcca tttttccaga agcattttgt
    15541 caaaggtcta acaatcggcg gagtaaaaga ataaagaaac aaaaatataa atcataaaat
    15601 taggaggaag aaaaatgaag ttatggaaga aactagcctt tacaggtgtt tcagcgttgt
    15661 tgttgggaac attagcagca tgcggaggtt ctggttctaa agaccaggca gaagctagca
    15721 attcggacac tttgcagatg tatcaaatcg gagataaacc agataatttc gatcaattga
    15781 tggaagtagc aaacaaacga atcgaaaaag agatcggtgt aaaagtcaat atcaattaca
    15841 ttggctgggg cgattacgaa aagaaaatga acgtcatcat ttcttccggg gaaaattatg
    15901 atatcgcctt tgcgaacaac tatgtttcca atgcccaaaa aggagccttt gctgatttga
    15961 ctgaattagc gcctaagtac gctgaaaaag cttataatga tttagatgaa gcatatatta
    16021 aaggaaatct ggtcaatgga aaattatatg ctttcccagt caatggaaat gtctttgctc
    16081 aacaagtact tacttttaac aaagctttac tagataaata cgatctttca attgatggaa
    16141 tccaatctta cgctgatgca gaaaaagtct tgaaggaatt ccatgaaaaa gaaccaaaca
    16201 cggcagcttt tgccattgga caaggcttca aagtccaagg gaactttgat tatcctttag
    16261 ggaatacgtt accatttgct attgatctgg atggagatga ttcgaaaatc atcaaccaat
    16321 acgataatga aacgtatatc ggactgttac gtacaatgca tagctggtac aaacaaggac
    16381 tcattccaag tgacgctgca acgagcaata aggattatcc actagaagga aatacttggc
    16441 taatgcgtgg agaaacacaa ggaccgtatg attatggcga tacaattttg acaaatgcag
    16501 caaaacaaga acttgtctca aaagcaatta ctgttccact aaaatctaca gctcaagcac
    16561 aaatggcaaa ttttgtcgta tcaaatactt caaaaaataa agaaaaatca gtagaacttc
    16621 tagggctgct taacagtgat ccagaattat tgaatgggct tgtttggggg atcgagggag
    16681 aagcatggga aaaagtcgat ggttcagatg ataaaatcaa attgctagat ggttatcagc
    16741 caaatacaca tatgtctgct tggaatacag gaaacaacaa gattctatat acacaagagt
    16801 ctattacgga tgacatgatt gccaaacgtg atcaatcgat tgctgatgca gaaacttcac
    16861 cgattttagg attcagcttc aacactgaca gtgtcaaaac agaattaagt aatatttcta
    16921 atgtcatgaa tcaatattta gatggattga atacagggac tgttgatcca gatgaaacat
    16981 taccgaaatt gaaagacgct ttggacaagg ctggctatga taaggtattg aaagaaatgc
    17041 aaaaacaata tgatgagttc cgacaagaat aataatgaat aaagacaaag gggttgtgac
    17101 aaaagacgtg tcacaacctc ttgttccgaa taaacggtgt tcaaaagcca gtttctcggt
    17161 attaagctaa atatatgtaa aaatgtggaa agccatattc acaaaatttt tcttactact
    17221 cgaaactaag cggctatttc acaaactctt ggtaaacggt gttcaatcag ctaaccctcg
    17281 gtattcgctc acaaaacgac aaaatatgag gaactatttt tccgtttgtt tctctaataa
    17341 tcaggtcaag gtgctgattg cccaaccttt ttttgtataa tgagaaagta taaacttaat
    17401 gtttggagga gaagatatgt tagggaaggt tttggaagag ttatttatcc ggatctgggt
    17461 cattataaaa ctaaccttgt atttctggat atatacattt gccggcggga tcatctttgg
    17521 attaggtgcg gcatggaaaa cagtcaatga actattttat ttgtacggat tcgagtataa
    17581 agaaatcaca ataaaaagag gctggaatat atataaaaga aattttttaa gaggcaatct
    17641 gctatttagt ttatttttat ctggtactgc tttattgagt tacaatttat atttatctgt
    17701 tcaaatcaaa gggctcattt ttttagctat tgattttatt ttactttttg ccttgctctg
    17761 cctatttgct acttatcaat acagtatgat tttagatagt gaatatacga tctctgttcc
    17821 taatctgcta aagctaagtt ttatttcggt tttttccagc ttttcgtctt ttctaaaaat
    17881 aattattggt agcggtataa ttttaggcgt gacttggcaa ttcaaaggat tgatcctgtt
    17941 tggtgtcatt ggtcttttga cggtttggaa tggtacgatg acaacacatt ggcgtgaaga
    18001 attagataaa cagttggaaa gttatgaata agtttcggat tctattttca aaaggaacct
    18061 tactaaatcg tctgttgcgg aaatacgcgg ttatcatgat cggtgtaacg atgactgcta
    18121 ccgttatttt tagtatccat acttgggaac aaaatcaaaa acaggcagaa aacatgacaa
    18181 gtgatgccgt tcaaagcaca agcagaatgc tgaatgataa aacaaccttg agtaggatca
    18241 tcaaaaacca gcttgtaggg aattccgaaa agatcgaaaa tgtgacgacc tacttaacaa
    18301 aacccataga tcagtatctg atgtatgtat acgaacagca aaacagtacc gatgaattag
    18361 tttctttccc aaatcaaatc aaagatctct acgcaaatta tgaagaacta agtgcgatat
    18421 atatcgtgtt gaatcaacta ccggagtatt atgaatcttc tagagaaaac aaaggtgggg
    18481 aaataaaaaa ggggacacca aaattactag acgcctttta tatcaagttg cctatcacac
    18541 aaggaggagc tgagtcagga agtattttta ttggttttca aaagaaagaa ctagatatga
    18601 ttttagaaaa tctaacgtca ttcaatggac tctctttata catgatttct ggaacgacaa
    18661 atcgactata cacattccat gataataaaa tagctaaaga aacgtttgaa agacaagaaa
    18721 tgatcatcac agagagtctg aagaaaaatt cacaattgcc gattgacgat ctggaaaaaa
    18781 acaattttgt tcaacaccaa gaactgtctg atgattatcg tatattagct gtactagata
    18841 aaaaaagtgt aaaaatcgaa acgggaaaaa atcttgcccc gctgatcatt ggtatcatta
    18901 ttttagacaa tatactgctt atctttcttt ataaaacatt caaacgatat tcacagcaag
    18961 tatctatcgt catgcaggcc atggatcaaa cagcagcagg gaatttagag acgcggatcg
    19021 acataacaaa cacggaatat gaactgaggg aactttcaat cggtatcaac gaaatgttgg
    19081 atagcatcaa tcagtttgta gaagacatct acaaattaga aatcaaacag caagacgcac
    19141 atatgcgggc gcttcaagca cagatcaatc cacacttttt atataataca ttagaatata
    19201 tccgaatgta tgcaatcagc gaaggaagtg aagaattagc agatgttgtg tatgcgtttt
    19261 ccgctttgct tcgaaacaat acgaatcaag agaaaacgat cactttaaaa gaagaactgg
    19321 acttttgcga aaaatatgtc tatttgtatc aaatgcgtta tcctaatcga gtagcctatc
    19381 attttatgat cgatcctgat ttggaaaaga tcgaagtacc taaatttgtg atccagccat
    19441 tagttgaaaa ttattttaaa cacggtattg attttactcg attcgataat gcgctaagtg
    19501 tcaaagtctt gcaggaaggc aaacgggtaa ggatcatcat caaagataat ggaaaaggga
    19561 tgactgaaaa acggctaaaa cagattgaag aaaaactgtc tcatcctaaa gtggaacttc
    19621 acggttctat cggtctgcaa aacgtgaatg aacgtttacg tgccagcttc ggcagcagtt
    19681 attatatgtc gcttgagaat aatgagacag gtggactgac cgtttctatt acattcaagg
    19741 agggatgagc gtgtatcaag tgttattggt agatgatgaa tacatgattt tgaatgggtt
    19801 aaaaaagata attgattggc aaagtttagg ctttcaaata gtagcaacag cagagaatgc
    19861 caaagaagga ctagctgtac ttgaacagcg acagattgat ttagtagtaa cggatgtgac
    19921 aatgccagaa ataaatggac tggaatttat tgaagcggct caaaaagaac gtcataattt
    19981 tgaatttatg attttatcag gataccaaga attcgattat ttgaaaggag gtatgcaatt
    20041 aggcgccgtg aattatttga tgaaacccgt gaataagttc gaattggttg aaagcctcaa
    20101 gaaaatcaaa acacggttag atcagcaaaa tgaacaaaaa aatcaacaag aaatctatca
    20161 agaactgtta ttcagtcaat ggttgaatga agaattggat gaaataagtg aagaagaaat
    20221 aataggacaa ataagtgaga agcagcatag gatagtattg attcaactgg caagaactca
    20281 tgatactctt atcaatagat ggttgcaaga acggcaacaa atcttttact atcaacgaaa
    20341 ttacggagac cgtgtattga tcacgttgtt gttggaggca gatgaagttg atggcttctg
    20401 ttgttttgtt caagaaaact ttcctgatca ggaatggctg atcagtatcg gggaagagac
    20461 agattctttg gacaaaatcc ctgaaacatt ccaacaagcg aaagacaatc tacagttgca
    20521 tcaattttat ggagataaag acgagcatgt tttttacgca gtacaagcag aaacaaaaga
    20581 tcaaaccatc gatttttcaa gtttcagacg cgtgctgcaa aataaacaat tggaagtcgc
    20641 tcaaaaaatg atcactgatt tttttgaaca gtttcagtta gcagccatga tgccggaaga
    20701 tatacgatat tccagttttt tactgtttat ggagattcaa cgggaattga ttgatctgga
    20761 agatgaggaa tatttacaag gaatcgaaaa aatccatcag gcaaaaacgg tgcaagaact
    20821 tcagcagcta cttctgtcat tcatcaaaaa gcatcagaga cagaaaaagt acagtgacaa
    20881 tgtggaaaaa gtaatcgtaa tcctgcacca gcattatcaa gaaccactta cactcaaaga
    20941 agtgtcagaa aacctgcatt taaatgtcat gtatctagga cagctgttca aaaaagaaac
    21001 gaaaaaaagt ttttcagctt atttgaacca tttacgtatg gaaaaagcga aacaactgct
    21061 tttgcacagt aatcaaaaca tcaatgagat agcaagtgag attggctaca acaacaccac
    21121 gtatttctct aaattattca agaaaatcgt cggcagatcc cctaaagagt accgagaaaa
    21181 cagtggagaa tcataaaaga aaaagccaat gtaatgaaat aacttataaa aaattgtcta
    21241 gaaatctaat tttgggctgt tcttattact tgaggcaagg cagctttttc atgacctctc
    21301 gataaacgga gttcaaaagc cagtttctcg gtattaagcc aaatttatgc aaaaatgtgg
    21361 aaagccattt tcacaaaatt tttcttaata ctcgaaacta aacagctttt tcacgacctc
    21421 tccactaaac ggtgttcaat cagctgaccc tcggtattag ctcaaaaaat ggcaaaatat
    21481 gaggaactat tttgccattt gtctctctaa tactcaggtc agaacgctga tttcacaacc
    21541 tctcaataaa cggtgttcaa aagcttgcat ctgcggtaat aagaccaacc aaacgaaaaa
    21601 tatgaggatc tatttttgct tggctgttct tattacttga tgcaggacag ctttttcaca
    21661 acctcttgtt aattatagtt tgataaggaa aaaggcaaat cttttgcttg atatttctta
    21721 gggtgaggga tgatccctaa ttggtaagag atcgtgccgc ctttcagcaa atcttgatgg
    21781 gtaaaatata atttcgtata ttctctgtcc atccgtttga ttttatggat aaattgttgc
    21841 tgcggccgat taggcgttgc ttcaatcgtc aattcctttc catttgacaa aagcaaaaca
    21901 gccttgtcaa ttaatggcat accgataaca tattcaccgg ttccaggcgt gacaggataa
    21961 aatcccagac tattgaagat ataccaagcg gccatacttc cattgtcctc atctccaggg
    22021 taacccattg gagaatcgtc gaaggtctga gtcatcaact gtttaagcag tggctgtgcc
    22081 atttcaggtt gtcctatata actgaacaag taaggatagt gaaaacttgg ctggtttgaa
    22141 atagctaatt gtccaaattc tatcgcggcc atctcactca tttcgtggat ctcaaaacca
    22201 tatcctcctg ggtcaaacgt tgggggctga ttgcacagct caatgagttt ttctttatat
    22261 ctctcactgc caccatattt tccgatcagt cctgaaaaat cgtgataaac agcaaagctg
    22321 ctttgccaag cacttccttc tgcataatct tgtccccaac gattagctga aaatggttct
    22381 ctgaacttgc catctttatc ttttgaacgc ataaatcctg actcatagtc aaagatattt
    22441 tgatagttca atgattgctg ctgatatttc ttcgtcattt cctcttcttc tagtaccttg
    22501 gcaacttggc taatacaaaa atcgctataa gcatagtcta atgtgtgatt gactgattcg
    22561 tggtagtgat taggaacata gccatatgtc aagtaatctt ctgttccttg gcgtccataa
    22621 tttggatgtt tgctttgaac cgtagcacct ttttccattg ccgttagaaa ttcaggcatc
    22681 aagtctcgtc ggatatcttt tacagcagca tctgcaatga ccgcatcgat caatgtccca
    22741 ggcataagtc cacgttcatc aggagacagc cattttggca aatagccact ttctctatag
    22801 ctgttcaaaa aaccttccag catttcttcg tatttttcta cagcaatcaa agaataaaga
    22861 ggataaaccg ttttatacgt atcccaaaac ccattatttg tatagagaac accaggtttt
    22921 atagatcgac tagtcgtatc atagtggatt ttttgcccac taggatcaat ttcataaaat
    22981 gtttgtggaa ataaaaacac ccgatataaa ttatgataaa acgtccgcaa ttgttgttga
    23041 tcatggtgtg tgagctgtat acgatcaaaa taggactgcc attgttcttg actgtgagca
    23101 aggtaagtct ctttgctttg gttttgttct agttccaggt tataagctgc ttgttccgag
    23161 ctgataaaag atgtcccaaa tcggatagtt tgtttttttg tctgttcaaa atacaacggg
    23221 atcgagtcgc tatttttgtt ggaaacagct aactcatcaa ttgttaaagg atattcaaat
    23281 ctcaatataa aataaaaaga aaaatcagga tcttctgaac cagcaaaatg tgtgattttt
    23341 ccttccactg tgtaaggatc tataacaaag agctgatggc gtcccggaaa ggataaaagt
    23401 aaaccgctgt catttttgct gtaatcaata gataagattc cgccatacat gctaggtatc
    23461 aatgtagaac agatgccata acgtaattga cagatcgaca gatgagtcgg gcaaaatata
    23521 ctttcttctg ggcggtaaga actttgagca tggaaaaggg tgttctcctg tagttttcca
    23581 ttgatcggcg taaacagcat atgactgaaa tcacccatcc aaggacttgg ttgatgggta
    23641 agacggtagc cttgaaaaac gcggtcatca ggatgaaacc accagctgcc gttttgatcc
    23701 gtagtttgcg gtgcaaaata attcatccca aaaggaacgc cagtataagg ctggcagttc
    23761 ccattagaga aatttggctg attcgctgtt gcatgtctag tatctatttg accgatattc
    23821 attttatttc tcctttgttg attgtctttt gatgaatgaa actggaaatt tactatcgga
    23881 tggtcgatct tttgtctcta accaagtgac caaagcatct cccgcaagct tacctaactg
    23941 ttcaaaatct tgtgcgatgg ttgtcagtgg cgggtcaaca agagctgctg cttggatatc
    24001 atcaaatccg ataattgaaa aatcttcagg gatatgataa ccatgctgtc tcaagagatt
    24061 catggtctgg atagcgacaa catcgttttc acaaacaaaa gcagttactt catacttagt
    24121 gatataagaa agcaattcat ctaatgtcgc gcttttgtca tccagtgaag tgtggaaagt
    24181 caaaccagct ttgtccaatg cttcaaggta accaaggtaa cgctgtcgaa cggattgtgg
    24241 atgagtctgc gtaccaaaca aataagcgat ttttgaatgt ccctgttcga tcaaataaga
    24301 ggcagcaagc gcaccgcctt ggaaattatc ggaaagaacg gtaggaaaag gtaactcata
    24361 gattttttta tctaatatga cgacagggaa ttctttcaat gaaagctcaa aaagtaagtc
    24421 aagatgttgt tctgtatcaa gagcataata aatcaatccg tcaaattcat tgattagctc
    24481 ttttgctgtt ttattttgta gaaagtcaag cgtcgtcatc atgacatcaa tctgtttatc
    24541 ttgcatcaca gggttcaatc ccttgctaaa atcaccaaca gacaaatcag cgacatatgg
    24601 aagaagaaac agaatccgtc gtgtttttgt aggagaagca agaatctgtt ccggagaagc
    24661 ggatttaacg aaactgcctt tcccgcgaac gcgataaata agtcctaatt gctctaattc
    24721 agtcaatgca cgtttggaag tgattctgct tactttatag gtacgtgaaa gttctttttc
    24781 agttggtagt tggctgtcta caggtaattc accagttaaa atagccgttt gcaaatcatt
    24841 aaatattgtc tggtacaatg gttgagtcat ataaacacct cttaatgata tatcatattc
    24901 aaataatacc ataatggatg aaaaagtaaa taaaaagata ttttttgaaa gcgtagacat
    24961 aattggctgt atgaagtata atctttaaag atatatcaaa tagaaagaag gttttattag
    25021 tggtttataa agatatacca aaatcggtgc agaaatttat ggaagatatc acacaaaaat
    25081 gtggggatat ccatgcggac tgggcaaaga attttacagc agcatttgcc aatactctgt
    25141 tgacaacagt caaaaagcaa aatgacggaa ccaccttttt attgacggga gacattccag
    25201 cgatgtggtt aagagactct actgcacaag ttcgtcctta tttagtgatt gcaaaagaag
    25261 atgaggaatt agctgacatg atcagcgggt tagtcaaaaa gcaatttttt tatatcaata
    25321 tcgatccata tgcgaatgcc ttcaacgaag aagctaacgg tgctggccac caaacagatc
    25381 atacaaaaat gaacgattgg atctgggaaa gaaaatatga aattgattct ttatgctacc
    25441 caatacagtt ggcctatctt ctttacaaaa atacaggaaa aactgatcaa ttcaatcaat
    25501 cttttattga aggtatcaaa aaaatcttgg acgtgttcaa aacagagcaa gatcatgaac
    25561 actctcctta tacatttgta cgagacacga caagaaaaga agatacgctg atcaatgacg
    25621 ggcgaggtgc aaaaacagct tttacaggaa tgacatggtc tggtttccgt cctagcgatg
    25681 atgcttgccg ttatggatat ctggtaccat ccaatatgtt tgcggtggtc gtgttaggtt
    25741 atgtggaaga aatttttcaa acgatactcg atgataaaga aattgtacag caaacacaga
    25801 aattgaagaa agagatccaa gaggggattc agaatcacgg acgaacggtt aatcaaaaag
    25861 ggcaaacgat ttatgcatat gaagtagatg gaatgggaaa cgccacagtc atggatgaca
    25921 gtaatgtgcc caatctgatc gccgcacctt acttgggata ttgttctgaa caagaggaag
    25981 attatctgca aacaagaaga acattgttga gtaaagaaaa tccatatttc tatgaaggaa
    26041 agtacgcaaa aggaattgga tcctcccata cgccagaaaa atacgtttgg ccgattgcat
    26101 tagctatgga aggaatgaca acgtcagaca aatctgaaaa agagcggata ttgaatttac
    26161 ttgtagcaac tgacgcagga acacacctta tgcacgaagg attcgatgtg aacaatcctg
    26221 aaaattatac gagagaatgg ttttcttggg caaatatgat gttttgcgaa ttagtcatgg
    26281 attactttga tattagagta gaaaagtagg aggtatcgta aatgaaaaag aaagtatata
    26341 tcatttctca tagccactgg gatcgtgaat ggtacatggc ttacgaacag catcacatgc
    26401 gactgattga attgatagac gatctactag agctatttga agttgatccg tccttcaaca
    26461 gttttcattt ggatgggcaa acgattattt tagatgacta tcttcaagta aggccagaaa
    26521 aaagacaggc aatccaacaa gcaatcaatg aaggaaaatt gagaattgga ccattttata
    26581 tcttgcaaga tgattttctt atcagctcgg aatcgaacgt gagaaatatg ctgattggca
    26641 tggaagaaag ccgtaaatgg ggaacccctg tcatgttagg ctattttccc gatacatttg
    26701 gaaatatggg acagacgcct caattaatga aacaagctgg tatttcagca gcagcgtttg
    26761 gccgtggtgt aaaaccaatt ggctttgata atcaagtttt ggaagcagaa aactattctt
    26821 cccaatactc agagatgtgg tggaaaggcc ctgatcaaac agcaatcttt ggtctgctat
    26881 ttgccaattg gtatagcaac gggaatgaga ttccagtaga aaaagaagca gctcttgcct
    26941 tttggaagca aaaattagcg gatgcagaac aatatgcctc tacgaaccac ttattgatga
    27001 tgaatggtgt tgatcatcaa ccggtacaaa aagatatatc aaaagcaatc catctcgcaa
    27061 atgaactgtt tccagattac gagtttattc attctaattt tactgactat ctggaagcag
    27121 ttcaaaaaga tgtgccagaa gacttaggct cagtagaagg agaattgaca agtcaagaaa
    27181 cggatggctg gtatacttta gccaatacag catctgctcg ggtttactta aaacaatgga
    27241 ataccaaggt ccagcgacag ctagaaaata ttacagaacc acttgcgacg atggcttatg
    27301 aagttagtgg aaactatccc catgatcaat tagattatgc ttggaagaca ttaatgcaaa
    27361 atcatcctca cgacagtatt tgcggttgtt cagtcgattc tgtccatcgg gaaatgatac
    27421 ctcgttttga aaaagcagat gaagttggaa agtatcttgc acaagattcc ttagaacagc
    27481 tgaccgcagc aatcgatacg accggattcc caaaagacag ctttccattt gtcattgtaa
    27541 atactgcagg aatggataaa actggtgaag cagaaataac catcgaatta gaaagaaaaa
    27601 gattcgctga aggaatacct gaacaactat atcaagagtt agaaaatttg cctaaacgta
    27661 agtatcatgt agaaacaaaa agtggggcca ctataccagc tattttatct gaagaaactg
    27721 tccaatttgg ttatgatttg ccgaaagacc gtttccgtgt gccatatatg gcaagaatga
    27781 tcaaagtgac tttaccatta gaaaacatgc cagcattttc ttgggaaaca ttcgcgcttg
    27841 tagaagggga agcgaaagca gaagaaaagg aaacaatgat tcatcagagt ggacgaatca
    27901 tcgaaaacgg ccctctacat ttgacgattg aaaaaaatgg taccattaca atggaagatc
    27961 gcaaaaacaa acgaaagttg aataatctgc atatttttga agatatcggc gatataggaa
    28021 atgagtatat ctttaaacaa cctttctgtg atcagccaat cctatccagt aacaaagaaa
    28081 actctgaagt aaaagtatta gtagatacac cggaaatagc aaaaataagt attctacagg
    28141 aaatggaaat ccctgtttct gcagacgagc gtttagaaaa agaacagcag atggttgtgg
    28201 aatttcgtta tcgaaaagca gaacgatcca aagaaaaacg aatccttcaa ataaaaacaa
    28261 tcatgacaat cagaaaagat agtaaaaaaa tcgactttga aacgactctc gacaatcaga
    28321 tgaaagatca tcgcttacgt gtcttgtttc ctacaaagtt acatgtggaa catcacgaag
    28381 cagatagtat ttttgaagtg gtgaagcgcc caaaccatgt gtctaaaagt tgggaaaacc
    28441 ccacgaatcc gcaacatcag caagcatttg tcaatattca tgatgaagag tacggtgtaa
    28501 cagtcggaaa ctttggtttg aatgaatatg aagtaacaga agacggacaa atcgcagtga
    28561 cgctacttcg cagtgtggga gaactaggcg attggggata ttttccaaca ccagaagcac
    28621 agtgtctagg tgagcatcgg ttcaattaca gcattgaact acatggacca gaagaaaaat
    28681 tctctactta tttgcatgca tacgcagcac agattccttt ttccactcaa caaatcaaac
    28741 atcatgaagg aacattgatt tcaaaacaac aatatttaac gataaaaagt gaaacgtttg
    28801 caattacagc tttgaaacgc agcaagttta gtgataaagt agtagtaaga ggtttcaata
    28861 tgtctagtca tttagaaaaa ttggaaataa ctaaagacaa tggaaaaact gtcatattga
    28921 atctgcttga agaaccaacg aagcaagcgg ttgtcccaat aatccaacct tatgagattc
    28981 ggacaatcgg attcgaggag gaaaacgaat gtatggcgga atagaagcag gcggaacaaa
    29041 atttgtgtgt gcggtttcag atggtggaga aattttagaa aaattaagta tccctaccac
    29101 aacaccagaa gaaacattgg atcaggtatt tgaatttttt gatcgttatg aattgaaggg
    29161 aatcgggatt ggctcattcg gcccgattgg tatcgacaaa acaaaagata attacggata
    29221 cgttttggca actcccaaaa aagggtgggc acaattcgat tttcttggca caatcaagga
    29281 acgctacaat attcctgctg cttggacaac ggatgtcaat gctgctgcat atggggaatt
    29341 gaagcaagga gcagcacaag gaaaaaatag ttgtatttac cttaccgtgg gaacaggaat
    29401 cggtgcaggt gtcgtgatca atgaagaaat tttttcaggg atcgcacatc ctgaaatggg
    29461 acatatttgg gtgaaacgcc atccagatga ccattatgaa ggtacttgtc cttatcataa
    29521 agattgttta gaaggattag cagcaggacc gtctattgag gcacgcacag ggatcaaagg
    29581 gcaagatctg ccgcaagatc atcctgtttg ggctattcag gcttattata ttgctcaagc
    29641 actgatcaac tatacgttga cgttagcacc tgaaaagatt attttaggcg gtggggtaat
    29701 gaatcaggat catctccttc aaaaaatccg tcagcaattt gttgaattga tgggtggtta
    29761 tatggaaaca cctcaagtaa gtgaatatat cgttcgttgg ggactgccga atgaaagtgg
    29821 gattatcggt agtttgctgt tagcagaaca cgaacatcaa aagtaagcag acaaattaca
    29881 caaaaagaac agagtcttat cattatctgc tgataagact ctgttctttt ttatttctat
    29941 tcccataatc caggtaaagc tttaccgact attttttctt gagtaaatgg atggatcatg
    30001 tgcagttcta atgcatggag catcagtcgt tcagctgttc tattttggta gagagggtct
    30061 ccaacgatcg gatgtccctt atgagctaaa tgaactctga tctggtgtgt acgtccggtt
    30121 tccaaaacac aatagacttg tgtttgttca gaaagtactt tgttgacagt gacatgtgtc
    30181 aaggcgctct gaccattttt atcatctacg accctttttc tccgatcatg gcgatctcgt
    30241 ccaattttat tggtgatggt catatctttg gcaatttctc cccatacgat ggcttgatat
    30301 ctccgataga tcattttttg ttccaacatt cttccaagta tcggcaagac aaatggattt
    30361 tttgcaaata aaacggcacc gcttgtttct ttgtccaagc ggtggacaac gtaaggtcgt
    30421 tgctgtttag tgtcgagata agcagctaaa tgattgagta aggtactgtt ttctgtcggt
    30481 tcatttggat gagtcttcat tcctgcaggc ttatccacta taatcaaatg ctcatcttca
    30541 tataaaacac ggatgtgctt cgcatctcct aattgaactt cttgataatt ataatctgtt
    30601 tcttcgaata acaatgtgat ctggtcattg ccctttactt catgatggaa caaagcaggt
    30661 tgttgattga tccaaacgtt tttacgtata cgcaaaaagt gtcgaacctt tctaggaact
    30721 aaccattcct tttcaagcaa gtcgcgaatt gttgtttcag gatggttcat aggtaaagtg
    30781 atcgtgattt tcacaagtgt actcctttca gtgcaattgc agactaattg taccattttt
    30841 aaaacatatt taagaaaaaa cttacatttt tctgttcatt cttatgttac tctacttgca
    30901 ctaacgtaaa aaaacatatt gaactacaac ttaagcagga agaaaacact ctttcactat
    30961 ggtaaaataa gacgattgaa tcaaatagac aggagaagaa gcaaatggat tttcaaagca
    31021 ttttagaaaa gataaaagct gctttgatca gtttttggcg ttggatcaga ccatatctcg
    31081 gacaattcca tcgatggcga aagcgcatct ggaaaaaata tcatgtgaac aaattgattt
    31141 tgctgattgg tttgatctgt gtcttagtga caagtatcta cctctttatt ttagcaaaac
    31201 aagcaaatgt cgaaacgtta aaatcaggat taagccaatc gacagtcatt tatgataaga
    31261 aaaacgaaga agctggcaca ctgtacgccc aaaaaggaac atatgtagaa ttaaacgcga
    31321 tttctcccta tatccaagat gcagtaatct cgacagaaga taggaatttt tacgagcatc
    31381 acgggtttga tatcaaaggg attgcccgtg cagcagtccg aatggtcttg tctggcggaa
    31441 catcaggggg cggaggaagt acgatcactc agcagcttgc caaaaatgcg tatctgacat
    31501 tagaccaaac gtttgatcga aaagcgaagg aattattttt ggcaatcgag atcgaaaaga
    31561 agtacagcaa agaagaaatc ttgaccatgt atcttaacaa tgcctatttt ggcaacgggg
    31621 tatggggtgt ccaagacgct tccagaaagt attttggagt agatgcaacg aatgttacgc
    31681 tgtctgaggc tgctactttg gcaggaatgc tgaaaggtcc tggtatttat aatccgatcg
    31741 atcacccaaa aaatgcgaat gatcggaaaa acacagtact aagtgtaatg gaagaaaacg
    31801 gaaaaatcac aaaagaacaa gaacaagcag aaagccagac agatatcagc gcgtatctta
    31861 atgacacata cgataattca gattctggtt atcgttatcc ttattatttt gatgcagtga
    31921 tcgatgaagc agttaacgag tatggtcttg atgaagaaga tgtcatgaat aaaggctata
    31981 aaatctatac agctttagat caggattatc aaaagcaatt agaagctact tatcaaaaca
    32041 gtgcattatt cccagcaaat gcttcagatg gtgccatggt ccaatctgct tcagtagctt
    32101 tagatccgaa tacaggtggt gtacaagcac tagtaggacg aagaggagat catgtcttta
    32161 gaggattcag ctttgctaca cagatgcgtc gttcaccagg atcaacgatc aaaccattaa
    32221 gcgtatatac accagcactg gaagctggat acaagccttc cagtatcttg aaagacgaac
    32281 cgcaaagtta ttacgatgcg cataattttg acgggacata ccaaggagaa gtaccgatgt
    32341 atcaagctgt tgcacaaagt ttgaatttgc ctgctgtatg gcttttgcat gagatcggtt
    32401 tacaaaaagg atatgataaa gcagaagaat tcggcctgcc tttagcaaaa tctgataagt
    32461 attatggctt agcccttggt ggactggaaa aaggagtatc tccattgatc atggcgggtg
    32521 cttacagtgc atttgccaat gatggtcaga tgtatacgcc gcatcttatc acgaaaatcg
    32581 ttgattcgac tggagcggtg atcgtcgata acacaaacaa aaaaccaaaa caggtcatat
    32641 caaaggaaac agccgatgaa atgaccagca tgcttcttgg tacattttca aatggaacag
    32701 cggctgctgc gaatcctgca ggttatacga tagcagggaa aacaggaacc acagaaacta
    32761 atttcgatgc cacaaaagca aatgaccaat ggatgatcgg ttacacgcca gacgtagtga
    32821 tttcgacatg gatgggttac gaagaatcca gcaagctgca ctatttggaa gggacaagtg
    32881 gaaatgttgt cgggaaagtc ttcaaatcag cggcagaagg catccttccg tatacttcaa
    32941 aaacaggatt tagtgtagca gatgcctatg ccacaggagg acaagtggta gctgcagatc
    33001 aagttggaaa cacaggaaat tctaatggag agtctggaaa ttggcaagat aacttgaatc
    33061 aatatggtga acaagcaaaa gaagggctga aaaacttcgg cgatatggtg aaagaaggcg
    33121 ttcagggaat tggtgaagcg gctaaagacc tctggcggaa atatcaagga gagtagcccc
    33181 tttgaaaacg aataacccca tgttacaata gagagtaact actaaaagaa aagagggcat
    33241 aacatgtcaa acatctacga tagcgcgaat caaatcgaac gtgagatccg tgaattacca
    33301 gaattcaaag cgttgaaaga agcgtacgag aaagtacaag caaacgaaga agcacacaaa
    33361 ctgttcaaag acttccaagc gatgcaaatc gaattacaag aaaaacaaat gagtggacaa
    33421 gagttcagcg acgaagatgc aatgaaagca caagaaatgg ctgtgaaaat ccaatcagaa
    33481 gaagtaatca atgacttgat gcaaaaagag caaggcttca gtacaatcat caatgacttg
    33541 aaccgtatca tcatgacacc agttcgcgac ctttacagcg aataaaacta ttttatgaaa
    33601 caacagaggg tgtgaaagag acgttcggcc ttgagcgatg acaaaattgc ttcttattgc
    33661 cgataggctg tctcttgaac accatttacc aagaggtcgt gagcctaccg tttaactctg
    33721 agttacaagg aacaatttca aaaaacagct tctcatgttt gatgactatt gtgacttgtg
    33781 accgaggagt tggcaggaga acaccgttta ttcagaaata ggagctacga caaaagtctt
    33841 gtcatagctc ctattttttg tcattcattt ttcaagcggt agaaatagcc tttttgagct
    33901 aacagttttt cgtaagtacc tatttcaacg atttttcctt catcgatcac aacgatttta
    33961 tctgcctctt tgatcgttga aagccgatga gccacaataa aagtagtggg tgtttctgtc
    34021 aggtaattca gggcttgctg gatttttttc tctgactggt tatctaaagc tgaggttgct
    34081 tcatctagca aaatgatttt tggctgacgt atcaacgctc gtgcaatgga gatacattgc
    34141 ttttggccac cagacagctt gttgccatgt tcgcctacta aggtatccag accatcagga
    34201 agctggtcaa tcagtgaaga aagttgcgca gcttcgatga cttcatctaa acgttctttt
    34261 gatacgtttt tcaagccata agtgatgttc tctttgatcg aagcggagaa caacaaagtg
    34321 gtttgaggaa caacggctaa ataatttcgc acacttcgca tatccagttc atccaaaggc
    34381 tgatcatcta gaaaaatttt tcctgaagtc ggctggataa atccaatcaa taagttaagg
    34441 agtgtagatt ttccagagcc agatggccca acaaaagcaa ttgtttctcc agctttcacc
    34501 tctagagaaa aatgatcgat aatcggtttg agtgagtccg gataaagaaa agaaacatcc
    34561 tcaaaacgaa aatttccatc caaatgttgg atttttttct taccgtgata agtttctaca
    34621 tcgccagcaa tcaacagttc gccaatagaa gtgatagatt ccatgccttt tgaaatcgta
    34681 ggaatcagcg tgatcattcc agaaacggaa ttgacgatcg tcgtgaaata cgtctgatac
    34741 atcacgacat cgccgggttg gatgcgccct tgtaaagcta atatcccagt gaaaatcaaa
    34801 caaatcagct gaaatatctg aaaactagcc cagctgactg atccgaaaac agattgcagg
    34861 atatctaagt ggaatccttt ttcatagatt tgagtaaaat gcttatttaa gcgagttgtt
    34921 tcgtgttttt ctaacccatg agctcgagca atcggtgcta attcgatcac ttcgatcact
    34981 tttgcagaag tctcttccat ttcttgcctg aagtctgagt tggactgtct gatcctttta
    35041 cggaaaaaga gagtaacgaa tgcggcgaga ggaacagtca ataagaaaaa catgaaaaca
    35101 acaggacttt tataaagcgt gatacctaaa gctacgaata aattgacagc gatatttaat
    35161 ccgctgacaa atacttgaga ggacaacgtt tggatagctt ctacatcacg gatgatcttt
    35221 gactggattc gtccagattg caattctttt tgataaggaa tagatagttc ttggattttc
    35281 ttgataaggg tcgctcttag tccagcttct acttgacgga tcgaagtgct atggtatttt
    35341 acatgaaaat aattcgtcaa tatattttga ccaatcagac aagccatcaa aaaagcgttg
    35401 aaataaatgg cctgcaactg agaagaatct ttcgatgaag ctgcattgat tacattggca
    35461 atagcgatcg gcattaacca agatggaaga tgtttgataa taaaaaagag tcctgacatg
    35521 gcaaaacgcc aataatttcc ctgataaaga tgcagcaatg tctgaaaagc atttgcatga
    35581 tatttttgat aacttttctg tattcgttcc tccataatct tctccttaac aatcttggta
    35641 aataaactgc aagtgttcta ttgcttcagg aacataaatc tcaggagtag cttcaagagt
    35701 ggcgtacatt agaggacgct catatttgat ataatgcagt accgggcgga aatctaaaca
    35761 gcccttgcca acaggtacca tgcggatctt tccattttcc atgatatagt ctttgagatg
    35821 aagcgctgta atataaccat gtaaattgtc caaggcacct cggatcactt tttcctgatc
    35881 agcatgattt tctttatgca tcaaattcgc acaatctaaa atgattttta aatttggtga
    35941 agctacttca tcgattaaac gtttagctaa ctgatagtta tagattggat gattgatccc
    36001 tgcttcaaca gcaacagtta cgccaaaatt ttctgcatga gcgactaatt caatgataga
    36061 atttctggcg atctgataag cttcttcagt aaaattttct tttgtgtaac cgacagagac
    36121 actaccggtt tccgtagcaa tcatcttggc ttgataggca gagcacagag caatttgctg
    36181 tttgaaagtt gccaatgctt gtgcacgaac ttcttgattt ttgctggata ggttgatata
    36241 gcatcctaaa atggaaagtt cgatattgtt tttttggaag tattcaccaa aataggcagc
    36301 actcccaagt gtcatgtcag ttaatttaag ttgttcagga aatgcttttt gtggtgcaaa
    36361 ttggatatgg gacaagtgaa gctgtgatac tttttgaacg atttcttcac gatttcgaac
    36421 atctaaatcg tgggcacgga tgcctaaatt taatggaaac atctcaaact cctttttgta
    36481 aataattagc aagaggcaga cgggattctt ttatcaattc aacaattatt cgggcaactt
    36541 tatcagcgcc ttctggacta aagtgggtat tgtcttttag tccttctggg taatttttac
    36601 aagtatcagg cataagatgc aggtagaatt tttttgtctc ttcttttgag aaggtctgga
    36661 acaattcttg tgatcttgag aacacatcta aaagaggaat atggtgtttc tcagcaaatg
    36721 cacgcatcgc ttttggatag tctccaagta tatctggatt caatgttccg ttttctaagt
    36781 aatctcgtcg cgtcacagat gtcagtaaga ccatttggac gttcgctttt tttgcgcatt
    36841 caaaaaatat ctgcagattt tttaaatagc taccgaaggg aagcgtacct ctttcttctt
    36901 ggattttctg gtcgttatgt ccaaattgta caaataagaa atcattcggc tgaaactgtg
    36961 aagctagacg ggctaacctt ccttcctgga taaatgattt ggtgctgcgt ccattctttg
    37021 cttggttatt gatctggatc tcgggtgaaa aataagcaga gattttttct ccccaacctg
    37081 tctcgggacg cttgttttca tctttttctg ccgcagtaga atcaccggca atatggatag
    37141 taggcatgtt atctcttctt tcataaaggt ttataggcaa tgaagcagaa cagcaagaga
    37201 agaagacgga gcaa
//
LOCUS       DAYYCY010000008        37189 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000008 DAYYCY010000000
VERSION     DAYYCY010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37189
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.008"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            7..375
                     /locus_tag="ACHAZB_000325"
     CDS             7..375
                     /locus_tag="ACHAZB_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HFM8613036.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            545..976
                     /gene="mraZ"
                     /locus_tag="ACHAZB_000326"
     CDS             545..976
                     /gene="mraZ"
                     /locus_tag="ACHAZB_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFM8613037.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            997..1956
                     /gene="rsmH"
                     /locus_tag="ACHAZB_000327"
     CDS             997..1956
                     /gene="rsmH"
                     /locus_tag="ACHAZB_000327"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFM8613038.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            1961..2362
                     /gene="ftsL"
                     /locus_tag="ACHAZB_000328"
     CDS             1961..2362
                     /gene="ftsL"
                     /locus_tag="ACHAZB_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFM8613039.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            2363..4555
                     /locus_tag="ACHAZB_000329"
     CDS             2363..4555
                     /locus_tag="ACHAZB_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFM8613040.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            4581..5543
                     /gene="mraY"
                     /locus_tag="ACHAZB_000330"
     CDS             4581..5543
                     /gene="mraY"
                     /locus_tag="ACHAZB_000330"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFM8613041.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            5557..6918
                     /gene="murD"
                     /locus_tag="ACHAZB_000331"
     CDS             5557..6918
                     /gene="murD"
                     /locus_tag="ACHAZB_000331"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFM8613042.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            6944..8032
                     /gene="murG"
                     /locus_tag="ACHAZB_000332"
     CDS             6944..8032
                     /gene="murG"
                     /locus_tag="ACHAZB_000332"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFM8613043.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            8047..9267
                     /locus_tag="ACHAZB_000333"
     CDS             8047..9267
                     /locus_tag="ACHAZB_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HFM8613044.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            9423..10748
                     /gene="ftsA"
                     /locus_tag="ACHAZB_000334"
     CDS             9423..10748
                     /gene="ftsA"
                     /locus_tag="ACHAZB_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFM8613045.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            10771..12012
                     /gene="ftsZ"
                     /locus_tag="ACHAZB_000335"
     CDS             10771..12012
                     /gene="ftsZ"
                     /locus_tag="ACHAZB_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFM8613046.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            12029..12706
                     /locus_tag="ACHAZB_000336"
     CDS             12029..12706
                     /locus_tag="ACHAZB_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HFM8613047.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            12720..13319
                     /locus_tag="ACHAZB_000337"
     CDS             12720..13319
                     /locus_tag="ACHAZB_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HFM8613048.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            13400..13621
                     /locus_tag="ACHAZB_000338"
     CDS             13400..13621
                     /locus_tag="ACHAZB_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HFM8613049.1"
                     /translation="MVYALLSWFPGGYQSGLGRLLAKICEPYISLFDRLPLRIGPLDF
                     TILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            13670..14452
                     /locus_tag="ACHAZB_000339"
     CDS             13670..14452
                     /locus_tag="ACHAZB_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HFM8613050.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            14473..15180
                     /locus_tag="ACHAZB_000340"
     CDS             14473..15180
                     /locus_tag="ACHAZB_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HFM8613051.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            15442..18240
                     /gene="ileS"
                     /locus_tag="ACHAZB_000341"
     CDS             15442..18240
                     /gene="ileS"
                     /locus_tag="ACHAZB_000341"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFM8613052.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(18289..19815)
                     /gene="zwf"
                     /locus_tag="ACHAZB_000342"
     CDS             complement(18289..19815)
                     /gene="zwf"
                     /locus_tag="ACHAZB_000342"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFM8613053.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(19830..20477)
                     /locus_tag="ACHAZB_000343"
     CDS             complement(19830..20477)
                     /locus_tag="ACHAZB_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFM8613054.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(20661..20990)
                     /locus_tag="ACHAZB_000344"
     CDS             complement(20661..20990)
                     /locus_tag="ACHAZB_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HFM8613055.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            21167..21895
                     /gene="sfsA"
                     /locus_tag="ACHAZB_000345"
     CDS             21167..21895
                     /gene="sfsA"
                     /locus_tag="ACHAZB_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFM8613056.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            21911..22924
                     /locus_tag="ACHAZB_000346"
     CDS             21911..22924
                     /locus_tag="ACHAZB_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8613057.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            22924..24201
                     /locus_tag="ACHAZB_000347"
     CDS             22924..24201
                     /locus_tag="ACHAZB_000347"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFM8613058.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            24264..26966
                     /locus_tag="ACHAZB_000348"
     CDS             24264..26966
                     /locus_tag="ACHAZB_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM8613059.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            27118..27435
                     /locus_tag="ACHAZB_000349"
     CDS             27118..27435
                     /locus_tag="ACHAZB_000349"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8613060.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            27465..27785
                     /locus_tag="ACHAZB_000350"
     CDS             27465..27785
                     /locus_tag="ACHAZB_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFM8613061.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            27893..29353
                     /locus_tag="ACHAZB_000351"
     CDS             27893..29353
                     /locus_tag="ACHAZB_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM8613062.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            29421..29642
                     /locus_tag="ACHAZB_000352"
     CDS             29421..29642
                     /locus_tag="ACHAZB_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8613063.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            29673..29855
                     /locus_tag="ACHAZB_000353"
     CDS             29673..29855
                     /locus_tag="ACHAZB_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HFM8613064.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            29855..30268
                     /locus_tag="ACHAZB_000354"
     CDS             29855..30268
                     /locus_tag="ACHAZB_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HFM8613065.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            30391..31572
                     /locus_tag="ACHAZB_000355"
     CDS             30391..31572
                     /locus_tag="ACHAZB_000355"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8613066.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(31643..31807)
                     /locus_tag="ACHAZB_000356"
     CDS             complement(31643..31807)
                     /locus_tag="ACHAZB_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8613067.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            31961..32034
                     /locus_tag="ACHAZB_000357"
     tRNA            31961..32034
                     /locus_tag="ACHAZB_000357"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31995..31997,aa:Arg,seq:cct)
     gene            complement(32080..32193)
                     /locus_tag="ACHAZB_000358"
                     /pseudo
     CDS             complement(32080..32193)
                     /locus_tag="ACHAZB_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(32576..33808)
                     /locus_tag="ACHAZB_000359"
     CDS             complement(32576..33808)
                     /locus_tag="ACHAZB_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFM8613068.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            34062..34631
                     /locus_tag="ACHAZB_000360"
     CDS             34062..34631
                     /locus_tag="ACHAZB_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8613069.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(34809..35249)
                     /locus_tag="ACHAZB_000361"
     CDS             complement(34809..35249)
                     /locus_tag="ACHAZB_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFM8613070.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            35407..36171
                     /gene="map"
                     /locus_tag="ACHAZB_000362"
     CDS             35407..36171
                     /gene="map"
                     /locus_tag="ACHAZB_000362"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFM8613071.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            36203..37126
                     /locus_tag="ACHAZB_000363"
     CDS             36203..37126
                     /locus_tag="ACHAZB_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HFM8613072.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
BASE COUNT        12301 a         6436 c         7832 g        10620 t
ORIGIN      
        1 acgttcatgg gcacattcac accacttatc ttgttttggt atatttaccc ggtaatcgtt
       61 ttgtttggtt gccaatttct tgtaaaatta ttttcgttaa atcgtcgttt taaaataaaa
      121 gcccctgatt tggcgattcc ttttttgtgg ggcggtatgc atgcattgtc tagaaataca
      181 gggacaattt ctatcttacc gtttttcttt atttctgttt tgctaatggg aatttgtatt
      241 gctgttttcc aagcatatta ttatgaagaa attatttatc cgcgttattt caagatgact
      301 tggcgtttgg tttttctgtt gacaatcgtc ttgtacgctg tcttgatcat tgtgaacata
      361 ctttcttacc tatgaaaaaa atagaaaagc tgtaaaaagt cttttaaaag gctttttgca
      421 gcttgatttt taaaaaaatg tttatgaata aaaaaagttg ttcccggatt tcccagaaaa
      481 ggtggtagaa agtgggggat tgtggtagac taattttagt tagtggagga atggggggct
      541 ttcaatgttg atgggtgaat tccaacataa tattgatgca aaaggacgtt taatcgtgcc
      601 ctcaaaactt cgtgaagaac ttggcgaaaa gtttgttctg acacgcggat tggatggctg
      661 tcttttcggc tatccgatga gcgaatggga aaatctggaa gctaaactga acgaaatgcc
      721 gttggctaaa aaagacgcac gtacttttgt gcgattcttc tattcggctg ccactgaatg
      781 cgaacttgat aaacaaggac gaatcaatat acccagcacg ttaagaaatt atgcagcttt
      841 gacaaaaggt tgcgtagtca tcggtgtatc taaccgaatt gaaatttggg atgagacacg
      901 atggcaagat ttttctgcag cagcagaaga gaactttgac gaaattgctg aaagtatgat
      961 tgatttcgga ctataagaat aggaagaggc tgactaatga ctgaaacatt tcaacactat
     1021 acagtcatgt tgaaagaaac ggtagatgga ttgaacatca aaccggatgg cgtttatgta
     1081 gactgtacac tcggcggtgc cggacatagt gaatatttat taagccaact aagttctaaa
     1141 gggcatctgt atgcgtttga ccaagatcaa aaagcaatcg atcacgccaa gctgcgttta
     1201 gcttcttatg tcgaacaagg tatggtcaca tttatcaaag cgaacttcag agaattagaa
     1261 gaacgcttga gtgagcatgt agataaggta gacggcattt tgtatgatct aggggtttcc
     1321 tctcctcagc tagatgaagc agaacgtgga tttagttatc atcaagatgc accgttggat
     1381 atgcgcatgg atcagtcagc tcctttatcc gcttatcacg taattaatga atattcctac
     1441 catgaattag taaagatttt cttccgctat ggcgaggaga aattttccaa acaaattgct
     1501 cgtgaaatcg aacgagtacg aaaaacaaaa ccaatccaaa caactggtga gcttgtcgaa
     1561 atcattaaac aagtcatccc agctcccgca agaagaaaag gcgggcatcc tgcaaaaaga
     1621 atttttcaag ctgtgcggat tgcagtaaat gacgaactcg gtgccgaaga ggcctcttta
     1681 gaacaggcaa tccggttatt gaaagtcaat ggaagaatca gcgtaatcac attccattca
     1741 ttggaagaca gaattgtcaa aagtatgttt aaagaatata gtacggtaca agatctacca
     1801 ccgggacttc ctgtcatacc tgacgaattc caaccagaac tgaaagtcat tactcgtaaa
     1861 ccaatccttc cttcagagga agagctaaca gaaaacaatc gatcacgcag tgcaaagtta
     1921 cgtattgcag aaaaaataag aatgaaagag gagtagaatg atggcagaat taaagaaaat
     1981 gcaagattac gaatatgatt tacctgaatt ggtggatcag ccggaacaac aaatgcctga
     2041 ttcttcttcg aatcaagaaa tcattcttcc acaatcaccg gcaaaacgat taaagcagat
     2101 ttctgctttg gaaaaaattg ctgtggcaag cgtggcgatt gctattgttg ctttatgtgt
     2161 tttaacagtt atgctgcgca caaatatcag cggagctgaa aaagaaatca cgtcaataca
     2221 aacagaaatc aatgataaaa agcaagaaaa aacgagttta gaacaagaaa aaaacgaact
     2281 ttctcgtact gaacggatta aacaaatcgc agaagaaaaa ggattgtcta ttaatgacga
     2341 caatttaagg aaagtgaagt aaatgagtct taaaaataaa attcggcggt ttatggaaaa
     2401 gaaaaatcta aatccgatga acaatcgcaa aaaagtaggt atcattttat ttgctacgag
     2461 tattggattg ttctttttat ttgccgtccg tttctcgtat atcgttattg gaggtcatgt
     2521 tgctggtacc tcattagctg aaaaaacaaa acagttgtac caaggaagtg aagtagtaaa
     2581 agcaaaaaga ggaacgattt atgatcgtaa cggtgtggcg ctcgcagaag atgcctcctc
     2641 ttattcaatt aaagcgatac tctcaaaaac atatacctct ggggataaaa aactgtatgt
     2701 tgaagaaaag aattttgata aaatagcaga aatcctgcat aagaatcttt ctatagacaa
     2761 aaaagatgca ctgaatattt tagaggacgg cgcaaaaaaa gagctgtatc aagttgaatt
     2821 tggtagctat gggaaaaata tctcccaaga aaccaagcaa aacatcgaag cggacatgaa
     2881 aaaagaaggt gtcgctggtc tttattttgt cgaccatcag gctcggatgt atccaaatgg
     2941 cgtcttttct tctcatttta ttggatacgc agttccagac aaagatgaga atggcttagt
     3001 tggtaaacta ggtttggagt ctgcttacaa tgatatcttg agcggtaaag atggtaagat
     3061 tatttatcaa aaagataatt ttcaaaatcc tctgccaggt acagtggcag aagaagaaaa
     3121 agctgtcgac ggacaagata tctacacgac tttagacagc agactacaaa gttatttaga
     3181 aacactgatg gatcaagtaa atgaagaata tcagccagaa gaattgacag cagtcctgat
     3241 gaaagcaaaa accggagaaa tcttggcaat gggacaacga cctactttca atcctgaaac
     3301 aatggaagga ttgactggaa aagatgctat ctggcgtaat ttcttggtac aagatagtta
     3361 tgaaccagga tcaaccatga aagttttcac tactgcagct gctatcgaag aaggagaatt
     3421 caacgagaat gaaaccttcc aatctggaaa aatccaagta gaagatgcta cgatcaatga
     3481 ccatgacttt ggtgaaaaag gtgtgctgac catgcgtcaa gctctttcat ggtcaagtaa
     3541 cgtcgggatg gtcatactgg aacagcgatt aggcggacga tggtataact atttacaaaa
     3601 actgggattt ggacaaagta ctcattctgg attagatgat gaagtaaacg gtgctttacc
     3661 tacattaaat attgtcgacc gagcaatgag tgcttatgga caagcggtag gtgtgacgaa
     3721 tttccaaatg atgaaagctt tcacttctat tgcgaataac ggtacaatga ttcaacctcg
     3781 atatatcagc aaagtggtgg atccccaaac tggagaagag cgaacaactc aaacagaagt
     3841 tctagggcaa cctttttcaa aagaaacaac agaaaaagtc cgtgaatata tgagagatgt
     3901 tgtcgaaagc gaaaactacg gtagtgctta tggtgtatat agtgtacctg gatacaatgt
     3961 atcggcaaaa aacggaacag cccaaatcgc ttctgataca gggggatatc aaacaggaga
     4021 tacagcctac ctgtattcga ttgtggagat ggttccttct gaagatccag actatgtctt
     4081 gtatctaaca atgaaacacc ctaagacata tgatcgaatg gcattggcta aaatcgcaaa
     4141 tccattgatg aaacgggcaa tggattttaa agaaactgaa gaagattcag atacagaaac
     4201 aaaaacagaa aaagtatccg tagccgatta tcgtaatctt gaagcggacg ttgcagcagc
     4261 tgatgctcaa aaaagtggac tgcaacctgt agtgatcggt aatggtaaaa aagtacaaaa
     4321 acaatctact gccaacggtg atcagttgat ctctggagaa aaactgatcc tatatactgg
     4381 tggtgataag ctgatgcctg acgtaacagg atggtcaaaa gcagatatca tgaagctggg
     4441 gaaaatcctc ggtattgaag ttagttttga tggagatggt tattgtgtca aacaagaact
     4501 tgctccatat gaaaaaatca ctgaagacaa acttagtttt actttagaag aataagaaga
     4561 gaaataggag agaaaagagc atggaatgga cacaagcatt gattcccatt gtcagcagtt
     4621 gtgcaatgac aattgccgca atgccgctat ttattggtta ttttcaaatg aaaaagcaag
     4681 gtcaggcgat tcgtgaggag ggaccaaagt ggcacaatag caaagccgga actcctacaa
     4741 tgggcggctt agtgtttctg atcggctcta ttttgacagg aatttgggtt ggagcttggc
     4801 aaaaacagct aactcctact cttttcatct tgctttttgt attagcgtta tacggagtaa
     4861 tcggctttct tgatgatttt ataaaaatat tcaagaaaag aaacatggga ctgaactcga
     4921 agcagaaatt gattggacag attctagggg gaatcatctt ttatcttgtt taccgttctg
     4981 aaggttatcc aggtgtattg aatttcttcg gactagatct acctttagga atcatttatg
     5041 gtattttcgc tctcttctgg cttgttggat tttcaaatgc agtaaatctg acagatggca
     5101 tcgatggatt agttgctggc ttaggaagta tctcttttgc agcttacggt ctaattgctt
     5161 ggcatcagca tcagtatgat gtattaatca tttgcttaag tgttttagga ggtttactgg
     5221 gtttcttcgt ctacaacaga aaacctgcca agatttttat gggagatgtc ggttcattag
     5281 cgttgggcgg acttttagcg gcaatttcta taatgctaaa ccaagaatgg acattgcttt
     5341 tgattggttt gatctatgtt atggaaacag caagtgtgat gcttcaagtt acttccttta
     5401 agcttacagg aaaacgaatt tttaaaatgt ctccaattca tcatcacttt gaaatgtgcg
     5461 gatggtcgga gtggaagatt gatacagttt tttggttgat cagcattgtg acttcgttga
     5521 tcacattatg gatcgtttgg taggagatga cgggaaatga aaaaaatatc aacatatgaa
     5581 aataagaaag tgcttgtctt aggactcgca aaaagcggag taagtgcggc caaattatta
     5641 catgaattgg gcgcacttgt caccgttaat gatggaaaac cttttgacga aaatccggaa
     5701 gcacaagagc tgctttcttt agggataaaa gtaataacag gtagtcatcc aatcgaattg
     5761 ttggatgaag aattttcatt gatggtcaaa aatccaggga ttccctacag tcatccttta
     5821 gtagcaaaag cccaagaaat gggtattccg gtaattacgg aagttgagct agcttacgaa
     5881 gtagcggaat gcccaattat tgggattaca ggaacgaacg gaaaaacaac gacaacaacg
     5941 atgacaggac tactactgaa tgcaggagca gatcaaggaa ttgcacgttt agcgggtaat
     6001 attgggtatc cagcaagtgg tgtagcgcaa gaagcaaaat cagaagataa aattgtgatg
     6061 gaattgtcta gcttccaact catgggaatc acagatttcc gccctcatat agcggtgatt
     6121 accaatatct atgaagcaca tattgattat catggtacaa gaaaagaata tgtcaaagct
     6181 aaatggaacc ttcaaaaaaa catgacagaa aaagattatc tgatccttaa ctggaatcag
     6241 tcagaactac aagaattagc gcaaagaaca aaagcacgcg tactgccttt ctcgacaaaa
     6301 gaagtgttgg aagacggtgt gtatgctgac gattactcaa tatactataa aaaagaaaaa
     6361 ataatggaaa tatctgaact tggcgtacca ggtaagcata atgtagaaaa tgcactagct
     6421 gctatttctg tagcaaaatt gtatggtata tcaaatgaag ctatacgaga aacgttgcat
     6481 tttttccatg gcgtaccaca tcgtacgcaa tatgtaggag aaatccaagg cagaaaattt
     6541 tataatgatt caaaagcaac caatatttta gcgactaaaa tggctctaag tggatttgaa
     6601 acgtctaagg tcgtgttatt ggctggcgga ttagacagag gaaatacatt tgatgaatta
     6661 attccttcat taaaaggaat caaagcaatg gtcgtatttg gccaaacaaa agaaaagttg
     6721 atggatgctg gaaaaaaagc aggcatcgag acaatagtaa cagccgattc tgtagaacaa
     6781 gcagttcctt tagcattaga aaacagtaca gatggtgatg tagttttatt atcaccagcg
     6841 aatgccagct gggatcaata cccaaatttt gaaactagag gaaacagatt catggaagct
     6901 gtgaaccgat taaaatagaa aaagtagaag cgagcgataa aaaatgaaaa ttttggttac
     6961 aggcggagga acagggggac atatttatcc agctcttgcc tttgtcaatt atgtaaagac
     7021 tcaagaaccg aatgctgaat tcatgtatgt gggagcaaaa cgaggtctag aaaacaaaat
     7081 cgtccctgat acaggaatgc ctttccatac cttggagatt caaggattta aacgaaaaat
     7141 ttccatgcat aacatcaaga cggtccgatt gtttcttaag agtatccgtc aagcaaaaaa
     7201 aatcctcagt gattttcaac cagatatcgt tatcggtact ggaggatatg tttccggagc
     7261 ggtagtgtac gctgcagcca aaatgggtat tccaacagtt atccatgaac aaaacagtgt
     7321 accaggtgtt accaataaat ttttaagccg atatgtggat cggatcgctc tttcatttaa
     7381 agatgcggct tccttttttc ctgaaaacaa agcagttctt gtaggtaatc ctcgagcaca
     7441 agaagtagca gatactaaaa aatcagatat tttgaaaaca tttgatttgg atccagaaaa
     7501 gaaaacagtc ttgatttttg gcggtagtca aggagcactt aaaatcaatc aagcagtatc
     7561 agttttttta agtgaatata ttccttcttc ttaccaagtt ttgtatgctt caggagatag
     7621 atattatgaa gagatcctaa ataaaataaa tcctgaaata caaaatatta gtgtccagcc
     7681 ttatatcaaa aatatggcag aggtcatggc ttccagtgat ctgctggtgg ggcgtgctgg
     7741 tgcaacttcg atcgcagaat tcacagctct ggggttaccg gcagtgttgg ttcccagccc
     7801 atatgttacg aatgatcatc agactaaaaa tgcaatgagt cttgttcacg caggagcagc
     7861 aaaaatgatt gcagataatg aactaacagg tgaatcacta agtcagacag tcaacgaaat
     7921 catgggtgat gaagaattgc aaaagcaaat gtgccgagct tctaaagaac aaggaatccc
     7981 tgatgcttca aaacgtcttt atgacttagt aaaacaaata atcaaaaaat aaaggcggtg
     8041 gtcttagtga gtaagaaaaa agaaccaaaa aaattgtctc atccgtcttc tgatgaaaac
     8101 aatttgactc cttggcaaaa ggctaaccga aaatatctgg cagaacaaga gatgaaaaac
     8161 caagagccaa aaaaagaaga cataacatta gatacacccc tttcagattc tgaagaagaa
     8221 aaaacggttc aagaggaaca gcaagaagaa agccagtccg agaaaaaaat cgaatttttt
     8281 gaagagatca agcaaaagaa aaacggtgga ccatataacg gttctttttt gaaccggctg
     8341 cccaatttaa aaagtcaacg gcacaaagta ctatttcgtc ggttagcttt gatcatcact
     8401 gtattgggta taccgctgat ttttttgatc tattatgttt ctccttatag caaattacaa
     8461 gcagttgcag tatcaggcaa taagacagtc aacagccaag aaatcatctc tgatacaaaa
     8521 ttgtcattag gagaaaatgt ttggggacag tattttcatc gatctactta tatcgatcgc
     8581 cttaaaaaag cacagccaag aatcgaaaca gcaaatatac atttcaaagg gatgaatgaa
     8641 ttcgatttgg atgtaacaga atataaagag attgctttga tcgcaaaaaa caatcaatac
     8701 tacccagtga ttgaaaatgg aacagtatta gatgaaaaag tagcaaatcc tacaaaaaat
     8761 ctccctatct tggaagagtt caaagactct gcaaaaatca aagaattaac aaagcaatac
     8821 aatcagttat catcagaatt acaaaaagca atttctgaaa tcaagtacac gccaagagca
     8881 agcaataaaa atctgatcca attgaatatg aatgacggca atcaagttat tgtaaatatc
     8941 aataatcttg cgaaccaaat gaaatattat tcgcaagtag ccaaagatat ggatgaaaaa
     9001 ggtgtgatcg acatggaagt tggtatcttc tcgcatcctt acggtagcac tcaagaaacg
     9061 gaagaatcgg gccaatcgga agaaacaact gaaagtgaag aaaatgcgga tcaaacaaac
     9121 gaatcaagtg aagaggcaga aacgcaaaaa gcacaaacac ctggatcgtc tagcacaaac
     9181 ggatcatctg attcaaatga ttcaagtgaa acaagtcaaa tagaagataa tccaagtaca
     9241 actccgtcaa gtttaaatga aaattgataa aacaacttta aaaagtaaga atgtactttc
     9301 taaaaaccag taatttatgg tagaattgga agcagtgaat atatctatta ataagtatat
     9361 tttaaaacgg acgctagttg ttcgcttgta tatggaatag taagtaggag ggaatcccat
     9421 tcatggcaaa aacaggaatg tatgtaggcc ttgatattgg aacgacatct gttaaagttg
     9481 tcgtggctga atatatcgac agccaaatga atattattgg agtaggaaac gcaaaatcag
     9541 aagggattaa ccgaggcatt atcgttgata tcgataaaac ggtccaagcg atacaacgag
     9601 cagtacgaca agcagaagaa aaagcaggta tccagatcaa aggcgtaagt gtcggtttgc
     9661 cagcaaacat gcttgaagta gaaaattgcc agggaatgat tgcagttaac ggagattcaa
     9721 aggaaatcac cgatgaagat gtcagaaatg tggcttcagc tgcccttgtt cgttcaatac
     9781 ctcctgaacg tcaaatcgtg tcaatattgc cacaagattt tactgtcgat gggttcgagg
     9841 gaatcaaaga tcctcgtgga atgatcggcg taagattaga aatgtacggt ctgttgttta
     9901 caggacctaa aacgatcatc cataacatac gtaaatgcgt ggaaaatgcc ggattaatcg
     9961 tcaacgaaat ggtgattact cctttagcgc tgactgaatc aattctttct gacggagaaa
    10021 aagattttgg tacgatcgtc attgatatgg gtggcggcca aacaacaacg gctgttatgc
    10081 atgataagca attgaagttt accaatcttg accaagaagg tggcgaattt gtcactaaag
    10141 atatctctat tgtattaaat acgtcattta acaatgcaga agcgttgaaa ataaactacg
    10201 gggatgcata cccagaacgg acatcaccag atgaagaatt tccagtagat gtgattggtc
    10261 agtcagaacc agtgaaagtc gatgaacgct acttgtcaga aattatttca gctcgaatgg
    10321 aacagatttt caacaaagca aaagatgcat tggaccaaat cgaagcatta gagttgccag
    10381 gtggcgtcgt attgacaggc ggagctgcta gtcttcctgg tgttgtggac ttggcacaag
    10441 aaatcttcgg cgtaaatgta aaattgtatg ttccaaatca catggggctg agaaacccgg
    10501 tatttacaaa tgtaataagt atcgtggatt attcagcaaa cttgagtgaa gtgtatcaat
    10561 tggcaaaaat tgctgttacg ggtgaaacct cagcagctcg tcaaatggta gttgaacaag
    10621 aaacaacaaa cacatatgag agctatgaag cacctgaaga aacaatctac gatgagccgg
    10681 aaccaaaaga gtccggagaa aatgtaaaaa ataaaatcaa gggtttcttt acaaatattt
    10741 ttgattaatt catcctggga ggaataagat atggaattct caattgataa taatatcaat
    10801 gacggcgcag taatcaaagt catcggagtc ggtggtggcg gtggaaatgc tgtaaaccga
    10861 atgattgaag aaaacgttaa aggtgttgaa ttcattacag ccaacacaga cgtgcaagca
    10921 ttaaaaaatt caaaagctga aacagttatc caattaggcc ccaaatatac tcgtggttta
    10981 ggtgctggtt ctcagccaga agttggacaa aaagcggcag aagaaagtga acaatcctta
    11041 cgcgaagctt tagacggtgc agatatgatt ttcatcactg caggaatggg tggaggaacc
    11101 ggtacaggtg ctgcaccaat cgttgcaggt atcgcgaaag aattgggcgc attgactgtt
    11161 ggtgtcgtga cacgtccatt cacttttgaa ggaccgaaac gtggccgttt tgctgcagaa
    11221 ggtatcgctc gtttgaaaga aaatgtggat acattattaa ttatctcaaa taaccgttta
    11281 ttagaagttg tagataaaaa gacaccgatg cttgaagctt tccgtgaagc agataatgtt
    11341 ttacgacaag gtgtacaagg tatctctgat ttgatcacag cgccaggtta cgttaacctt
    11401 gactttgctg acgtgaaaac agtgatggaa aaccaaggta cagcattgat ggggatcggt
    11461 gtggctagtg gagaagatcg cgtagtagaa gcaacgaaaa aagcaatctc ctctccattg
    11521 cttgaaacat ctattgatgg agctgaacaa gtacttctta acatcacagg cggtttggat
    11581 atgacattgt tcgaagcaca agatgcttca gatatcgtag caaatgcagc aactggcgat
    11641 gtaaatatca ttctaggtac atctatcaat gaagaaatgg gcgatgaaat ccgtgtgacg
    11701 gtcatcgcaa caggtatcga tgaatcaaaa aaggaacgca agtctagccg accagctaga
    11761 caagcacaaa tgcaatcacc tgctcaaaag actgtattag atatggatca agctaaacca
    11821 acttcttcag aagaagaaaa cagttttggc gattgggata tcagacgtga acaaaatgtt
    11881 cgtcctagag ttgatgattc caattttgac aatatcgaaa aaaaagaatt tgatacattc
    11941 aaccgcgaag aaacaaaatc tcatggcgat gatgaattaa atacgccacc gtttttccgt
    12001 cgtaaaaaat aaaggagagg aagagcacat gattgctgac aacttgcaga aaatcaagca
    12061 agaaattcag gattcgtgtg ctcttgttca tcgtgatccg aaagaagtta cattagtagc
    12121 tgtaacaaaa tctgttgaat ccgctcaagc tgaagaattg gttagagctg gcgttatgaa
    12181 ttgtgcagaa aaccgagtag ataaattatt agaaaagaaa caagatttat ttgattttcc
    12241 tgaaataaag tggcatttaa ttggtaattt acagcgcaga aaggtaaaat tgattgtaaa
    12301 tgaaattgat tactttcatg cgttggacag tttaagatta gcagaagaaa tccagaagcg
    12361 gttagagaaa gaattatcct gttttattga agtaaatgtt tctggtgagg aatcaaaaca
    12421 tggaatttca ccagatgagc ttttaccgtt tgtggaatct ttagctgcct gcacaaaaat
    12481 caaggttgtt gggttgatga ctatggcgcc taaagaagct tctgatgagc aaatccataa
    12541 agtgtttggt acgctgaaag aattgcgtga tcttgttcaa gcaaaaggtt atgcccatgc
    12601 accatgtagt gaattaagta tggggatgag tcaagacttt caaattgcga ttgaagaagg
    12661 tgcaacattt atccgtgtag gaacagcgct attcaaagac gaataaagga ggaacaaaaa
    12721 tgccgctttt aaacagagga gcaattgcta attttttcgg attagctgat gaggaagagt
    12781 acgaatacca agattatcct gaaacaacaa aaaaggctgt acagccaact aaaaaaaata
    12841 caacacagaa tcaatcaact cattctgtat ctgctgcgaa tactcatgaa aaacaaaaaa
    12901 cggcgacttc acgtcctgta acgcatgaaa gtattcagcc ggctgaaaag aaaaaagtaa
    12961 ttgaactaca tcaatcacct acaaaaactg tagcgaaaga aagaacagct tcaagaacct
    13021 ctacacaacc taaagcaaat cttgctggaa aaatcactgt aatggaacca agagcttatt
    13081 ctgaagctat ggcgattgct aaacgaatca ttgctggaga agcaacctta gtgaattttc
    13141 atttagtaga agaaaaacaa gcgagaagaa tcgttgattt tctaacagga acagtttatg
    13201 cactagatgg agatatcaaa cgtgttggag atgagatttt tctttgtaca ccgactggga
    13261 tggagattga cagttcaacc gctcaatctt ttgcagatgc gaatttgttt gatctgtaag
    13321 aggaggaact acgatttata ctattgcact attacttcat ttacttcaac gggcagccta
    13381 tttctatagc attatcttga tggtatatgc attgctctct tggtttccag gcgggtatca
    13441 atcagggcta ggacgattat tagctaagat ctgcgaaccg tatatcagtt tgttcgatcg
    13501 tctgcctttg aggattggac ccttggattt cacaatttta ggggcaatcc tgatcttgaa
    13561 cttagccgta caaggtttag cacagatcgt tatatggata actgtccatt tagtttattg
    13621 attgaagcaa caaaatcttt attctcagaa tagataggag gggaaaattt tggatgcaaa
    13681 tgtataccag cactttcggg cagatgaacg tacatttatc gattcggtag gcgattggat
    13741 tgaacaggtt caaggacaat atgcacccta tctgaccgaa tttcttgacc caagacaatc
    13801 ctatatttta gaaacactga ttcgacaaag cagtgatttg cgtttcatgt tttatggcgg
    13861 atatgaacag gcagaaagaa aaagatgttt gattttcccc gattattacg aacctgtaga
    13921 ggaagatttt gaaacagcat tgtatgaaat ccattatcct agcaaatttg ccagcctaag
    13981 tcatgggaaa atccttggaa ctttagtggg aacggggatc aaacgctctt actttggcga
    14041 tatcatctcg gacggtgatc gctggcaagt atttattgca aatgaggtgg atcattttgt
    14101 tgctgcacaa gtaacaaaga ttggaaaggt tgctgtacgc ttggaaccaa tcaaatacac
    14161 agaaatgatt atgccgaaag atagctggac acaagaaaga ggaacagtta gttccatgcg
    14221 actagacagt gtgatctctg agatttataa tatttccaga cagcggtcca agcagctgat
    14281 cgaagctggt aaagtaaaag tgaactggac agaaaataca agacctgatt ttgtacttga
    14341 actattagat attatttcag tcagaggtta tggcagagtc cagatccaag aacttgaggg
    14401 gaagacgaaa aaagaaaagt acagggtact gttcggtgta ttacgtaaat aaaataaaaa
    14461 ggtggtttac tcatggcttt aactccatta gatatccaaa ataagacatt cccgacaaaa
    14521 atgcggggat ataatcaaga cgaagtagat gatttcttag atttagttgt tcgtgactat
    14581 gaggaattga ctcaaagaaa tcgagaatta gaaaaagcag tcaaacattc tgaagaaaaa
    14641 cttgagtact tcaatgaatt aaaagatgct ttgaaccaat caatcatcgt agctcaagat
    14701 acggctgata aagtaaaaac aagtgcaagc aaggaatccg aagtgatcgt tacttctgca
    14761 caaaacaaag cagatgagtt agttgcgaac gctgaaaaac gtgcacatca attaacaact
    14821 gatgcagaag aaaaagcaag aaaaatcttg acagatgcaa cagaaaaagc aagacagctt
    14881 gcgacagaaa ctgaagatct taagaaaaaa acacgagtat tccatcaacg tatcagcttg
    14941 atgcttgagt ctcaattaga acaagtgaaa agtccggaat gggatgaaat tttacaacca
    15001 ttctccagct acgtgactga cagccatgaa gtgatcaaag aagttttagc aaaacagctt
    15061 gacaatgaaa atgatactga tgtaaactca gacacaggag tcatagaagc acctatgacg
    15121 gcatttgata cacaggctat tgatatctcg atcgtgcctg gtgaaaatga aaacgagtag
    15181 aacagaaaag cactttgtta ttcttaaagc gagccgatga gagtggaagt tcggtaagac
    15241 actgcatggt gaaagatcct ctacttgaga cctgcttttc cgaaattaga gtaagaagca
    15301 gcggctgacc tcgttattgg tcacagagag tttgcactag atagatgcaa ctaaatttgg
    15361 gtggtaccac ggcatgttcg tcccttgcgg ctgaacatgc ttttttgtgt ccggaaaaca
    15421 aataaaggag tgtatggttg aatgaaaatg aaagaaacct tgcaattagg aaaaacaaaa
    15481 ttccctatgc gtggtaattt gccaaaccga gaaggcgagt ggcaaaaaga atgggaagaa
    15541 aatcgaatct accaaaaaag acaagagttg aacgaaggaa aacctagctt tgttcttcat
    15601 gatggacctc catatgccaa tggtaatatc catttaggac attcattgaa taaaatcagt
    15661 aaagacatta tcattcgttc aaaatcaatg tctggctatc gtgcaccata tgttccagga
    15721 tgggatacac atggattgcc gattgaacaa gtattggcta ataaaggcgt gaaacgtaaa
    15781 gaaatgtcca tggcagaata cttggaaaaa tgtcgtgaat atgcactttc ccaagtagat
    15841 aagcaacgag cagatttcaa acgcctagga gttgcaggag attgggaaca tccttatgtt
    15901 acattagatc cttcttatga agctgcacaa gtacgcgtat ttggtaaaat ggctgaaaaa
    15961 ggctatattt acaaaggatt gaaaccaatt tactggtctc cgtcaagtga gtcttcttta
    16021 gcggaagctg aaattgaata taaagatgta aaatctccat ctatttttgt agctttcaat
    16081 gtcaaagacg gcaaagatat tcttgatgaa gatacagctt ttgtcatttg gacgacaaca
    16141 ccatggacat tgccagcaaa ccaaggtatt gctgtaaatc caaaatttac gtatgtattg
    16201 gtggaagccg atggccgtaa gtttgtcgta gcgaaagatt tgctagaaac agtccaatca
    16261 gaaatcggtt gggaaaatgt aacagttcta aaagaatttg ctggacaaga aatggaatat
    16321 atgactgcaa gtcatccatt ctatgaacgt gaatcacttg tgattttagg cgatcatgtt
    16381 actttagacg ctggtactgg attagtccat actgctccag gacatgggga agatgactat
    16441 atcgctggta gcaaatataa actatctgta gtgtcacctg tagactcaaa aggattgttt
    16501 acagaagaag cacctggatt tgaaggtatc ttttatgaca aagcaaatcc tatgatcact
    16561 gatctattaa aagaaaaagg agctttgctg aagctagatt tctttactca tagttatcct
    16621 cacgattggc ggacgaaaaa gcctgtgatc tatcgggcaa ctccacaatg gtttgcatct
    16681 atcgataaat tccgtcagaa tattttggat gaagtagaaa aagtagagtg ggttatccca
    16741 tggggtaaaa cacgtttgta taatatgatc cgtgaccgcg gcgattgggt aatttcccgt
    16801 cagcgtgcat ggggagtacc acttccgatt ttctatgctg aaaatggaga agcaatcatc
    16861 acaccagaaa caatcgaaca tgtggcaaaa ttatttgctg aacatggatc aattatctgg
    16921 tttgaaaaag aagcgaaaga acttcttcct gaaggattta cccacccagg ttctcctaat
    16981 ggcgaattta caaaagaaaa agatatcatg gatgtctggt ttgattctgg ttcttctcat
    17041 gaagctgttt tacgtcagcg accagaatta tcattcccag cagatatgta tttagaagga
    17101 tcagaccaat atcgtggctg gttcaactca agtatcacga ctagtgtggc tatcaatggt
    17161 gtagcaccat acaaatcagt tatttctcaa ggatttgctt tagatggtga aggccgtaag
    17221 atgagtaaat ctttaggaaa catcattgca ccagaaaaag tgatcaaaca atttggtgca
    17281 gatatccttc gcttatgggt atctagtgta gattatgaag cagatgttcg tgtttctatg
    17341 gatatcttaa gtcaagtggc agaagtatat cgtaaaatca gaaatacgat gcgtttctta
    17401 ttagcgaata cagaagattt cgatcctaaa aaagatactg tttcgtacaa tgatttacgt
    17461 tctgtagaca aatatatgac cgttcgattg aaccaagtaa tcaaagaaat ccgagaagaa
    17521 gggtacgaca aatataactt catgcatatt taccgtacag ttatgaactt cttgacggtt
    17581 gatctatcgt cattctacct ggattttgct aaagatgtag tttatattga agcagaagat
    17641 gattatcaac gtcgttgcat gcaaactgtt ttctatcaaa cagctgtagc tttgacaaaa
    17701 ttattgactc ctatcattcc gcatacagca gaagaaatct ggtcattcct gaaagaagaa
    17761 gaggagtacg ttcagttaag cgagtttcct ggttatgaag agtttgcaaa ccaagaagaa
    17821 ttgatggata cctggactgc tttcatggac ttccgtgata atgtcttaaa agcattggaa
    17881 gaagcaagaa acagcaaatt gattggtaaa tctttggaag caaaactaac tgtttatcca
    17941 aaacaacaag ttcgtgaaat gttaaaagct ttggatgcag atattgcaca attattgatc
    18001 gtttcaccag actactttga agttatggca gcagatgaac aagtgccaga taacgctatg
    18061 gtatttgatg atgttgctat tacagtggaa aaagcagacg gggaaacttg cgatcgctgt
    18121 cgccaagttc gtaaagatgt aggagtagat gaaaaacttc ctcatctatg cggtcgttgt
    18181 gcaaaaatcg ttgaagactt ctatcctgaa gctgtagctg aaggattcga agaaaagtaa
    18241 aagggaaaag ggggtgtgac atgttctttg tcacaccccc ttttgttatc aatcgtttaa
    18301 ttttccacgt tcacgatacc agttatctgg ttgccagatc cattcgaatc cgtctttttc
    18361 aagtagttcg aaagctgctt taggtcccat tgatccagct ttgtagttgg gaaaatccgg
    18421 ttgtgtttgg tcccaagctt ttcgaatgac atccacgata tgccaagatt gagcgacttc
    18481 ttcccaatga gagaagttcg ttccatcacc attcaacgca tctaatagta atttttcgta
    18541 agcttctggg ctattttcaa ccatttctgc actatttcta aattccaatt tgattggttc
    18601 cgtttcaaat ccttgcccta cttctttccc attcaaggat aaagaaaatc cttcagttgg
    18661 ctgaatatag atcgttagaa cattaggtgg aagtgtcgta tcgtcacatg gttcatcgac
    18721 agatgttttg aatacattaa caggaacttg tttgaatacg atgttgatcc gagtcccttt
    18781 ttcagtcaaa cgtttgcctg tacgtacata gaaaggaaca cctgaccaac ggaagttatc
    18841 gatagtgaat ttgcctgcga cgaatgtttc agtctctgat tgagcatcca ctttgtcttc
    18901 ttcgcgatag cccttgaatt gtacaccatc caattcacct tgagcatatt gcccgcgaac
    18961 gaagttttct aacacttctt cttcgttata aatgcggata gctttcaatg ctttgatctt
    19021 ttctgtacgg atttcttttt cggaaaatgc tactggcggt tccattgcaa gtaatgctac
    19081 aacctgtaaa atatgatttt gcaccatgtc tttaagtgcg ccactatgat cgtagtagcc
    19141 cccgcgatct tctacaccga ggttttcgcc aaaagtaatt tggatattgt cgatatagcg
    19201 attgttccat tgagcctcaa aaatattatt ggcaaaacgg atagctgaga tgttttggat
    19261 catttctttt cctaaataat gatcaatcct gaaaatatct tgttctggga atacttctct
    19321 gatttgctgg ttcaattcaa aagcagactg gtaatcagaa ccgaacggtt tttctatgat
    19381 caaacgattg aagccatttt ttgtcataat attttgtgat ttcaaatgag aaacgatcgt
    19441 tccaaaaaat tgaggtgcca tagccaaata atagacacgg ttaccgttta attgatagtg
    19501 tccatctaat cggtcagaca attcttttaa tgtctgataa tgcgttgaat catttacatc
    19561 atgggattga tagtaaaaat ggcttgcaaa ggtagttgct tcttcatctg aaggattcaa
    19621 tgcacggatt gtttctttga caacttcacg gtagtgctca tcgctccagg gacggcgagc
    19681 ggttccgatt acagcaaaat gctcgcctaa atttcctttt ctgtaaaggc ggaacaaaga
    19741 cgggtacaat ttacgctggg ctaggtcacc tgtaccgccg aatatcgtaa acaatacatt
    19801 tttttcatta gacatgtgga tacactcctt tatattttat cgacaaaaat cgtgctggcc
    19861 gccttaaagc tgacggggat tttctttcta tcattttgga tagtgatcgg tccttcataa
    19921 gctgcgatgt caacgatctc atattcttcg ttgatattca ggtcaatagt gactaagtaa
    19981 tccaacaatt ctttttcatc aagcactcgt gcgatacgca cttttgtacc gacaggatag
    20041 gactccaatg tttgtcggcg ttcttcatgg acaacttcct cttttttagg aatcgatcct
    20101 ccatgaggac aatgttcagg atgattcaaa tagttttcta aatgatctgc aagatgttcc
    20161 gaagtcacat gttccaatac ttcagcatca tcatggactt cattccagct atagtctaaa
    20221 tgttcaacta aaaaaacttc ccataagcga tgtttgcgaa tcagactgct agctttttgc
    20281 agtccgcttg cagtcaattg tacaccttga taaggggaat gttctacaag ctcttctttc
    20341 acaagtttag aaatcatttc actgacagat gcagctgaga catctagact agatacgatt
    20401 tgtttattgt tgactttcgt tgtatctcca cctagttcta aaatgatttt caaataatct
    20461 tctcgatttg gtgtcatatt cgatcatcct ttcatggtaa aaaaccatca ttagtaagtg
    20521 taacaaaaaa aacgttaatt gactattttt ctaaataaaa aacaaaggat ttttagacgt
    20581 ttttacaaca aaaaacgaaa gatgatagag aagactcatt ctttcgttta taaaggatat
    20641 atatatacat tgggagggat ctatttttca gataacaact tatatgaagt gtctgcagat
    20701 tcgtcataaa gagtttgttc gggataagaa aagataagat aaaaaccata ctgatcacgt
    20761 acgactaaac gatattcatc tatttctacc acagatgtgc caagagactg accgttgatc
    20821 aaaaacgtgt aatcagatgt aatctgtaat tgttgttttt gctggctatc actaaagaac
    20881 catgtacctt gatactttga gagtccttct gacttttccg cttgtttttt ctttttcaag
    20941 taaataaaac ttgagagaac ggtaatgagt gaaccaagaa aatagatcat tttttttatc
    21001 accaaatcac tcctttatga gaaaataatc gcatagacat attctttctt ctttatttca
    21061 ttgtaccgaa taaaagctaa aatttctcta agactttcga ctggtttttt tactgttttt
    21121 ctacaaatta tgataggata catagtagga agaaaggtga cataatttga agtatcagca
    21181 agtggaaata gcttttttta ttgaccgccc gaatcggttt atagcacatt gtatgaaaaa
    21241 agacggagaa ataattattg cgcatgtcaa aaatacaggt agaggaaaag aggtcctgat
    21301 accaggcgct gaagtggcta ttgtatttgc tcctggacct aaacggaaaa ccgcctatga
    21361 tctgattgct gtaaaaaaga agggtgactg gtttaatatc gatagtcaat tgcctaatcg
    21421 gctggcaatt gatggtattt tagacggcac gatcctttta ccaggattaa ctggtccatt
    21481 tacggtatac aagcgtgaag taacttatct tcactccaaa tttgatattt atttagagac
    21541 ggtggtcgga caaaaagcat ttgttgaagt caaaggtatg acacttgaaa ataaagcgat
    21601 tggtgctttt cctgatgcgc ccacactcag aggattgaaa catataggag aattaacgta
    21661 tgccgcacaa gatggttatg ccgcttatgt cttgtttatt gctcagttcg aacatcttca
    21721 tctggcaacg atccatgaag aaatgcaacc agcactagct gatatggttc gacatgcgca
    21781 gcaaagcgga gttcaaatcc tcgcctataa ttgtcaagta tcggcagatg aggtaactgt
    21841 caagcaggcc attccatttg atgtagatat cccttttgaa gatcctatac aatagaaaga
    21901 aggtaaaata atgatcaact taggtattat cggaacaaat tggattactc atcaatttgt
    21961 ccaagcagct ttaagcacaa aaaaatatga attgacagcg gtatattcta gacatttgga
    22021 aaaagcacag gaatttggtt ctcattatga aggggatatc gaattcgcaa cagatttgaa
    22081 gacttttttc aatattactc atatggacac catttatatt gcttctccga atagtcttca
    22141 ctttagtcaa gctaagcaag caattttggc aggaaaaaat gtgatagttg aaaagccagc
    22201 tttttcgacg ccaaaagaaa tggatgaaat catccatttg gcaaatgaac acaatgtgta
    22261 tttttttgaa gcagcaagaa atatccatga atcaggtttt aaaaagattg ccgattactt
    22321 accattaaaa gacgagataa tcggtgcaaa tttcagctat atgaaatatt cttcgcgcta
    22381 tgaccaagta ttagatggag aagaaccgaa tatcttctcg actcatttct caggtggtgc
    22441 attgatggat ttaggtgttt accttgtata tgcagctgtt gcttggtttg gaatgccaaa
    22501 agaagtccat tatttcccaa gaaagatttc tactggtgta gatggtcttg gatgggggat
    22561 cttacgttat gagaattttg atgtatcgat ccagccaggt aaaatcggtg attcttattt
    22621 accgtcagag atttattttg actcaggaac gctagtgatg aatggagtca atgcaattga
    22681 acaagcacaa tttcatgatc gcgcgcataa agaaatcatt gatttggcgg taacagctgc
    22741 agaaaatcca atggcagatg aagcagagga ttttgccaat gtcatggctc atccgaaaga
    22801 tccagcttgg ggattgttgt atgaagagtg gatcgagcta gctagaaatg tgaaccaagt
    22861 gatttatgat ttaagaaaaa atggcggcat caaattcgat gcagataatg aggaagattt
    22921 ttaatgaacg aatttgtaca agtatttccc gaaaattggc agaaaaaata tgaggataaa
    22981 gggtttatct caccttctct tattcaacga gcagtttttc agccactaag taataaacaa
    23041 agtatagtag cggtttctcc tacaggttca ggtaaaacac tggcttatct ttggcctcta
    23101 ctactaaacg tggaaccagg tgaagcaagt gcgttggtca tttttgcttc ttctcaagaa
    23161 ctagctattc aagtagcaga tgttgcacgt gaatggggaa aagacaaaga gttgaaagtc
    23221 caatctttgg tgggcggagc caacgtcaaa cgtcagattg agggtttgaa ggaaaaacca
    23281 gaaatcttga ttggtacacc aggaagaata ttggaattga tgaaagcaaa aaaaataaaa
    23341 gcacatcaag tgaaaacaat ggtatttgat gaagcagacc agctatttga cgctgggaac
    23401 agccaaatca ttgatcaaat tcttcatcaa gctccaacag agtatcagct agcttttttt
    23461 tcagcgacag cagatcgttc ccttgaaagc attgaaaaaa tcactgggaa aacgtttgag
    23521 acaattgacg tgacagcaga ggacgattct cgaaaaggac tgagacatta ctttttacgt
    23581 gtaccgatac gaaagaaaga tgactacttg agacgtctca cgcatataga agattttcat
    23641 ggtctgattt tctttaatca actgactgaa ttaggcgtaa tggaagaaaa attgctgtat
    23701 agaggtgtac cggtaggaag tctggcctct gatcagaaca agctactccg aaaagcagcg
    23761 ctagaacaat tcaaaaagaa aatggtcaga gcacttttga ccacagatgt ggcggctaga
    23821 ggtttagata ttacaggtct tccttatgta gtgaatgcag aagttccgtt aagtgaagaa
    23881 gcttatttgc atcgttcggg aagaactggt cgaatgggta atgaaggaat agtaattaca
    23941 ctcgtacaag ataataatct aagagattta aaacggcttt tacgtccatc aaatataaca
    24001 cttgaagaga tttttttata cggaggtaag ctgcaaactg agcagccaac caaagaagat
    24061 gcagctcctg cagtaaataa aaagtatgcg aaaacttttt ctggaaagga gcagaaccca
    24121 gaatataaca aagaaaaaat aagaaaaaaa gttaaaaata aatcaaaaaa agagagaaat
    24181 aaaggagcaa ggcgcaaata agcgcctttt tatgctatga ttatgaaaac gggaacaaaa
    24241 ttggttgcca gaggaggggt tatgtgaaag aacgtcaaga agagatcttg cagctattga
    24301 cagaaaacca acaaggattg acagcaagtg atgtagctga gcgcttaact atcgatcgaa
    24361 gcaatgcaag ccgatattta agtgaactat acaaggccca tcatattgta aaaaccgctg
    24421 gccgaccagt cgtttactct ttgccaacgg aaaagtcaaa atctgatgaa gtgcatgtcg
    24481 actcttctac gcaagtgact tttgaaacac tcgttggaga aaatgattct ttaaaagtca
    24541 gcatacagca ggcaaaagct gcgattcttt atccaccgcg ggggttgcat acgattattt
    24601 ttggagaaac tggtacaggg aagtcgatgt ttgctgaatg tatgtatcat tttgcaatcg
    24661 attcagagat gctttctgca gatgcgccat ttgtttcttt caattgtgcg gattatgctc
    24721 aaaatccgca actgctcttt ggacacatct ttgggatcaa aaaaggggct tacacaggag
    24781 cagctcaaga tagtcccggt ctaatagcca aagcagacgg cggtatattg tttttagatg
    24841 agatccaccg tttaccgccg gaagggcagg aaatgctgtt tactttcatt gataaaggta
    24901 tttaccgtcc gcttggagaa agtgcacaag tacatgaagc atctgttcag atcattgggg
    24961 cgacgactga atcttcagaa agtttcttat ctacgttcaa tcgacggatt cctatggcaa
    25021 tcaccttgcc gaatcttgct tcacgttcac tggatgagcg ttatgaaatc atctctcttt
    25081 ttatcaaaca agaagcgaat cgtttgaatc aacgtatcga tatcgaaaaa gaagcgattc
    25141 ttgccttcat gctttatgat gcggaaggaa atatcggtca agtgaaacga gacttgaaac
    25201 tagtctgtgc aaaatctttt ttacattacc gtactcatca tgagaaaaag ctaatcattc
    25261 gaaaagaaga actttccctt caagtacaaa aaggattact gaaaatcaag gaaataccgg
    25321 aacgtctcga ccgattcatg gacagcaaaa gtcagtattt gacatttgaa ccaggatttg
    25381 ctgatgttgt ctggtcacaa gatccagaac gaaacatgca ggtctataac gatattgagg
    25441 aaaaagtcac ttctctttcc gaaaccggtg tggagaacat tgatctagag accctcattt
    25501 caaaagatgt ggatgcttat ttccagacat atgtcaaaga attgactaaa agttcgattc
    25561 ccaaagatct tttgccagat gaaatctggc ggttaaccaa tcaattgtat gatgtggctg
    25621 aaaaacggtt agaccgtatt tataatgaaa aagctcgttt tgcttttgct ttgcatctgc
    25681 aaagtacatt ggatcgtgtg agagaaggtc atatgatcgt ccacccagat ttgaataacg
    25741 tacggaaaaa ttttaagagg gaattccagg tagcaatcga tctttcaacg atcattgaag
    25801 aagaagaaca agttgagatt ccttttgatg aaatcggctt tattagtatg tttctttcta
    25861 tcaatctagg agaaagtgag cctgttaaag aaaacaaagt agatgttctc atattgatgc
    25921 acggcagaca gactgcttca agcatgttgg agatggcaca agaactatta ggaacaaaag
    25981 tagggaaagc ttttaatatg ccactggtta tggaagttca aacaatgtat gaagaagtgt
    26041 tacaatatgt caaaaactat caagagcatt tgacgaatgg cctactcttg ttaacagata
    26101 tgggatcatt aaacaccttc agtgatttga tttatgaaga gactggcatt cggacgaaag
    26161 ctatcacgat gacaagtacc atgattgtga tagaagcgat acggatggca agtgttggaa
    26221 gaagcttaga agatatttat caaaatatcc agctttcctt tgaatcgatc gtacgtgatc
    26281 agttcaaagc ggaaaagtct gaaactttac agcgtaaaaa agccgtaatc gtgacatgct
    26341 ttactggtga aggagtagct gccaaattat atcagcgtat tatgccagtg attaatcaaa
    26401 ataaggtaga actgatccag atgcagtttt tagaacgtga atcattcaaa aaacacatcg
    26461 atgccttatt ggaagaatat gagatcaaag caattgcggg aacagttgat gttgattatc
    26521 aaaatatccc atttttttca gcctatgacg tttttgatga tgaaaaacta aatattttaa
    26581 agcgtatagc tgatgatgag atcccgacag ctaaaattgt cgattcactt gctggatcat
    26641 taagcgatgt cttttctatt gatgaactga tccgttcatt gcaaaagaca gttcagcaga
    26701 tccagaccga tttgtcgatt atcgtggaac ctggagtaga tactggaatc gttatccatt
    26761 tagccttcat gttagacgga ttgatcaaac aagaaagcca tcggcaattt gagaaactgt
    26821 ctattttctc aaaaatttat cgtttggaaa tggatatcgt tcgggcaaac ttgatggcac
    26881 tagaacgaaa atatcaggta aaaattccag aagatgatat cgcatttctt acacaaatgt
    26941 ttcttgaaaa taaaataaaa aaatgaacac actaatcata ctgtttacac actaaacatc
    27001 tgtgtgcgca ctataaaaaa gagctaaaaa gcatgaaaac gctatttcag ctcttttttt
    27061 tattggcatg cttcttgcgt tatataaaag tgtaaaaaaa taaaaggagt gttaaaaatg
    27121 gcaaaaaaaa caatcatgtt agtttgttca gcagggatga gtacgagttt attggtcaca
    27181 aaaatgcaaa aagcagcaga agaaaaaggg atggaagccg atatttttgc ggtctctgcc
    27241 tcagatgcag ataataattt agaatctaag aatgtcgatg tattattatt aggtcctcaa
    27301 gtacgtttca tgaaagctca atttgaacaa aaacttgcgc ctaaaggtat tccattagat
    27361 gtcatcaata tgcaagacta tggcatgatg aacggagaaa aagttcttgc acaagcagaa
    27421 aatctaatga aataaatcaa gtatgcagtt gggggtataa caacgtggaa gatcaacaaa
    27481 atctagaagc catcatgggg ttaatcatgt ttggcggtaa tgcaaagagc gatgcaatgg
    27541 aagcgattgc ggctgctaaa atcggtgatt ttgagttagc ggataggaaa atcgctgatg
    27601 cagaagaatc acttatacaa gcgcatcatg cacaaacaga catgttgaca caagaagcac
    27661 aaggaaatca tatgcaagtg acattattga ctgttcacag tcaagatcac ttaatgacat
    27721 ctattgcgtt tactgattta gcaaaagaaa tcatcgaact ttatcgtcgc gtatacagcg
    27781 aataaataca acttcttttt tgaacggaag aagaaaaatt tttgaacgga ttttttattt
    27841 tgttataagg agaactctct ttatataaaa aacttatagg aggaagtaaa aaatgaacgg
    27901 actatcaagt tggatggaga aatacatcct gccggttgcc gcaaaaatcg gagcgcaaaa
    27961 acatttagtt gctttgcgtg atgcttttat cggcacactg cccgcaacaa tggcgggatc
    28021 agtcgctgta atgatcaatg caattattcg tgatttaccg caacagttta acagtaccta
    28081 tgctgacagt ttagcagcag acaaaggaat ctttgccgta ttgaatgtca tcatcggaat
    28141 caatggtttt gtctggaacg gtacactagc aatcgccgga ttgatctttg ccttttcttg
    28201 ggggtataat cttgcccgag cttatggagt aaatgatcta gctgggggga tcgttggttt
    28261 agctaccttg attcaaggta tttcctttgg tccagcatta tcagcagcat tagatgtgaa
    28321 tgttccttca aatgtcgcag atgcaatcaa tagttctgag attggtgcaa cgattgcaga
    28381 tggcacactg tcagttggat tatggggttg gctaaaacta gatcatttga atggaaacgc
    28441 ttattttaca gttatgatca tgggagctat ttcagtcatt attttttgta aattgatgct
    28501 tgccaacatt acgatcaaaa tgcctgattc agtaccacca gcagtatcta aagcatttgc
    28561 agccatttta ccagcgacga tcgctttata cgtcattgct attatcaatt atatcgtagg
    28621 tcgtgtagct gatgggcaac tgatcattga tttggtgcaa aaatatattg cagagccatt
    28681 cctaggatta tctcaagggt tgggagcagt attgatcgtc acagtatttg ttcaagtatt
    28741 ctggttcttt ggtattcatg gaccaaatgt attggcacca gtgctagagg gaatctgggg
    28801 acaagcacaa ctaatcaata ttgatatttt ccaaaaaggt tataacggaa aaactggtac
    28861 accagccgtg ttagatgcaa tcgaagatgg aaaagcttat atgtgggtcc gcggatcgtt
    28921 cgatgctttt gcatggtttg gcggatcagg cggaacgatc gtcttactga tagctatctt
    28981 gattttctct aaacgtgcag attatctaac agtagctaaa ttatccattg ggcctggtat
    29041 cttcaatatc aacgaaccag tgatgtttgg tctgccaatc gtattgaatg caatcatgtt
    29101 tatcccattc ttgattgctc cagtagtagc aacagcaatt ggatggacag ctacttatct
    29161 tggcctagtc gctccggtat cgcagcaagt cacatgggta gtaccgccgt tcttattgtc
    29221 attcttagca acaggagcgg attggcgggc accgatcgta acattagtat gtatggtcgt
    29281 gactttcttg atttgggcac catttgtcat ctctgcaaac aaaatggacc ctgccgcagg
    29341 cgaacaacaa taagatgaac gtgaggagcg ggacgcttta agtttcgttc ctcataatga
    29401 attttcgagg aggaactgaa atgattacaa ttgccagtgc aatgcaacaa gatgaaatca
    29461 aagaattgtt agaaggatac aatgaagaag gaatgacttt tacatttaaa gaaaaacaag
    29521 gaatcaagct gttctttgaa acaagtgcag aagatccgga agttgcggct aaaaaagcaa
    29581 aggaactaat caaagcacag ccttggggaa cagtccttta ttttcaatca acagctgttt
    29641 agaaaatggt ttaaaaagat aggatggttg agatgataaa aaacggttta tttttatgta
    29701 gtattggttc tttgatcgtg ttgtatagtt tgaatccaca ttcgcaaagg tatgatttgt
    29761 atagtatggt cacaggtctt tttctaacag gattaggaat ctatttcttc atgaagggga
    29821 aaaaaagaga agaagcaaag gagaaaaata actaatggaa atcacgaaaa aattaaatat
    29881 tccagcatcg tttttttacg ataaagttat ggactctgtc cttttcgatg tacgtaaagc
    29941 gaccggcaaa tcagtaacta gaaaacagct gaaaaatttg gagtacgtca aacaattctc
    30001 gcaaaataac cgggctcgca taaaaatcga agaagtcatt gaaaaccagt cttataagtt
    30061 tcgtacatcg acaacgaaga atgaattcgt cgttcattat caaatcaaac cattggacga
    30121 ttcgacctgt gaggttcatt acacagaaaa aatggagtct tatggcatgc ttcaaaagtt
    30181 gaatgatatg cttttaggaa caatcttgat gtattttaaa cggagacgtt tcaaaaaaat
    30241 gctagatatg atcgaagcaa cgtattaaat caatgaaaca tggtactatt tagttattga
    30301 aaaatgaagt aagctagaag gaatttttca taggaagata gatctttcct gcgaatcctc
    30361 actagctttt ttgtaggaag gggcagcaaa atgacaaaca aagtaagaga gctaggtgta
    30421 tcttcgattg aggaaatgtt tgatttagcc acttatgcat ttaatggggc agatacacct
    30481 gaaaggcgag aacgttttcg tacacttgcg gagaattctt ggaattacgg attttttgat
    30541 gaggaggatc ggctaacaag tcaagtcatg gcgacccctt ttccagtgaa tttttacgga
    30601 caagagtacc taatggccgg cattggctat gttgcttctt atccagaagc aaggggacaa
    30661 ggcgggatca atcagatcat ggaaaagatc cttgaagatt gccgtgatcg taaagtaagc
    30721 ttatcttatc tggcgccatt ctcctatccc ttctatcggc gctatggcta tgaacaatca
    30781 tttgataaaa tcagttatca actaaacagt cgtgatgtgc cgtttatcaa aaaaaaatct
    30841 ggaaaaataa agcgtatgga ttttgaagat gcaaaagctg caatcagaag aatttatgaa
    30901 cagatgccgg aaaaccaacg agctggtctg aagcgcgaag actggtggta tacgtataaa
    30961 ttcaagcaaa aaggcaatta ccagtttgct ttgcattttg atgataatga ggaagcaacg
    31021 ggatatatcg tttatcagct tgcaacgtct tcctttatta ttgcggagtg gggatttttg
    31081 gatcatgatg ctttttgcag ccttgttcgc ttcgtaagtt ctcacaatgg tgcttttgaa
    31141 acatttgaat attcatgcgg ctacggcggt aatgatcaaa attatctatt agaaaatcca
    31201 tttgcttctg taatgatcac accttatatg atggcgagga tcgtagatat ttctttgttt
    31261 ttggaaaatt atccatttaa aaagagacaa gctgcatttg ccctacaaat agaagaagat
    31321 cattatgcga aatggaatga aggaatcgtg gaagtaacaa tcgaaaatgg aaaaaatcat
    31381 ctctcaaaag tagaacaaac aaaactccca atcgttcgag gaagtatcca acatatcacg
    31441 cagctattat tagggtatcg taaagtggaa gagttggtat tccaagaaag aatacaggtg
    31501 acaaaagaat atgaagaaac gttaagctca atcttgccac agcaacagcc aattcttaat
    31561 gactattttt gataaaaaac gggtgatgac aatatttttg tcaccaccct tttgtttttc
    31621 atttggcatt tgaaggaaaa tgttaaagta aatcctcttt tttcgtaaat ttgaattttt
    31681 tgttttcttt tgttttaggg ttttcttttt tgtggtcttg gattgcttct ttcgtaatct
    31741 tttcccgatt ttttttagat gaatctaagc gttctgcgta tactttttct tttggcttat
    31801 cattcattgg aaaggacctc cctacattat ttcattctac ctctagttta tcatctgtta
    31861 aggactagtg gaaacgtttt gcctaactta taagctgatt tttttagcag ataagcttgc
    31921 aagtaaaaaa caaacaagct acaattgatt tattgaaatt gttcctgtag ctcagtagga
    31981 tagagcaatc gcctcctaag cgataggtcg ctggttcaat tccagtcagg aacgtacaaa
    32041 taacccctgt atcctttgcg gtacaggggg ttattttgta taagtagaca ttgtagtttc
    32101 taaactgcta tgtcctaaac gtttcgatac gctaagtata tttactcctt gataaagtaa
    32161 aatagatgca tgcgtatgtc ttagtccatc aataaacagt cgaagagaaa atagggaaaa
    32221 attgattact aaagaaaaac ttactaggtc gacggtgtag ttgattttca tacttcaata
    32281 gattcgaatt aaacgatttc gattggctta taccagtcct atccaacgaa agatatatcg
    32341 ttcttaactc tagcggtata acttgaagct ttacgtaaag ccaatgggaa tgaactggag
    32401 atgacttttt attattttta gtaatagctg atgggttaat acttacaact ttccttttca
    32461 aagtaccata taaaaaaacg gtggggttgt ttagcgaaat aaaggatcta agccaatagc
    32521 ttagatcctt tattctatgc tttatattat tttttgcatg tgtgcgaaag tatactcaag
    32581 cccaatcgcc atttcggaaa atcggtactg tagagccatc ttcacggatt ccgtcgatat
    32641 ccatcttgtc agatcctacc atgaaatcta cgtgggtttg acttctattc aatccagctt
    32701 ctgctaattc ttcatcggac atttctgtac cgcctttcac actgaaggca taagcagaac
    32761 ctagtgctaa gtgattcgat gcgttttcat caaataatgt attgaaaaag atgatgccag
    32821 attgagaaat aggtgaaggg tcaggtacaa gtgcgacttc tcctaaacga cgcgcacctt
    32881 catctgtatc aagcagtttt gctaagacat cttctccttg ttcggcagaa aaatcaacaa
    32941 cttttccatc tttaaaagta aatttcatac ccgagatgat ggtgccagca taactcagtg
    33001 gctttgtact tgaaatgtaa ccatctactc gatgactgtc aggtgctgtg aacacttctt
    33061 ccgtcggcat atttgccatg aatttttctc cacgagcatt atagcttcca gcgccttccc
    33121 aaagatggtt tttaggcaat ccgataatga tatctgtacc gggagcagta tagtgaaggg
    33181 cagaaaattg ttctcgattc aattcttctg ctttttttgc taatttttca tcatgcttct
    33241 tccatgctaa gacagggtct tcttcataga cacgagtcgt tttgaaaatc tgatcccata
    33301 atgcctcaac ttgtttttct tctggcagtt ctgggaagac ttttgctgcc cattgtttac
    33361 cagccgcagc cacgacagtc caactgactt tatttgcttg agttgccttg cgtaaattca
    33421 ttagtgcttt gccattcgct gattgataag acgctactcg atggctatct acaccagcaa
    33481 atgcatcggg atcggcagaa acaacgctga tcctgcttgc tcctttttca agccattcat
    33541 ccgcttgatc gattttagat tgtggtacat tctcaatacg atccgttgca gcatgtaaaa
    33601 gaaattctct ttggatctgg tcgtcagtcc attgaacgat aacctcagcg gctcctagtt
    33661 tataagcttc ttgtgtgatc aatcgagcaa ggggtgcctg ttccacgctg atctgtaaaa
    33721 caactgtgtg tcctttttct gtggctacgc cagtttctgc aattaagcgt gcatattttt
    33781 ttagaagttc attaaaatca gataacataa taaaaattcc tcctcaccat tttttctggt
    33841 aactattagc atgataccat ttaattatga ttcaaacaat gaggctttaa ataaaaaaag
    33901 gaggttattt ttttaaggtt aagtttcatg atatatgtta ctacttgcta tttcagcttg
    33961 tgaagaaaat caaaaactaa ttttgcatat caaaaaaaca tgttacaatg aaattaatca
    34021 atggtggaag cgttataaaa aaattaagga gattaaaaaa tatggcaaga aaaaagacga
    34081 ttactagaga acagattttg gcagctgctt atgaagtagc tgccaccgat ggatttacac
    34141 gatttactgc gagaaacatc gcgaacaaaa tgaattgttc cacgcaaccg atttatcttg
    34201 agtttaaaaa tatggatgac ttgagacaag ctctttttga gcagatctat gatcatttac
    34261 aatatgaagt gttcactgtt gagcatacag ggaatacgat tatcgatctt gcttttaatt
    34321 atatccattt tgcaagaaga gagaagaagt tgtatcgttc actgtactta gaagaatatg
    34381 gtggaggaaa agagatgcag aatttctctt atacttactt tgtgaataca gtgaaacgcg
    34441 atccagctta tgtgaatctt tcagaagagc agatcgaatc tttgtataat gggacatgga
    34501 tcatagctac tgggatcgca gctttgatgt cttcaagcat catccatcca actgatgaac
    34561 agattgaaaa gatgatccaa gacagtatag atgctatctt gaaattcaaa gaaccaatcg
    34621 aaatcgattg aatatactca aaagttccac aaatcagtag gtaacaagag atgtgtcaaa
    34681 ataaggaaaa gttcgttttt cttacagcgt atgcattttt aactcccgaa gaactcttcc
    34741 atgaatgcaa tgaataaaag agccatacag attgaataaa tctggatggc tcttttattg
    34801 caagtatatt aaatttttgc gactagttct ttagcgaaag cttctaaatt ttcgatatct
    34861 tcttcttcag cattcagatc cactttaaca ttttcggctc cttttgatgc gcctgttttt
    34921 gcaaaagcag catcgaaatc atccaccgat ttgcagaatt catcgtaaaa agtatcacca
    34981 gatccgcaga caccgtaaat ttttcccaat agatccaatt cttgcaagtc ttcataaaag
    35041 tctacgatct catcaggcag atcgccatca tcgtatgtat aagtagctac tacgcaaata
    35101 tctgcctcct cgaattcatc tgcatctact tgggtacatt cgttgatttc gacttcgata
    35161 tccagatttt caaatgcttc tgcaacaata tcggcgattt cttcagtgtt tcctgtcatg
    35221 ctagcatata cgatttttgc taaagccatg atgtgcctcc tcgtttataa ttatttaaca
    35281 attatgaatt ataccaaaac atcattgaaa agaaaataaa acctaaagac acctggggaa
    35341 tgtcatttgg ctaaatgtat gccgctatgt taaaatgaga gggatttgta aaaaggagac
    35401 gaaaatatga ttactttaaa atcagcacgt gaaattgaaa tgatggcaga atctggagct
    35461 ttattagcag atgtacataa aaatctgcgt gattttataa aaccaggaat cacaagttgg
    35521 gatattgagg tatttgtcag aaactatatc gagagtcatg gcggaattgc tgcgcaaatt
    35581 ggctttgaag ggtatgaata tgcgacttgt atcagtataa atgatgaaat ttgtcatgga
    35641 ttccctcgta aaaagccttt gaaaaacggt gatttggtaa aagtcgatat gtgtatcgat
    35701 ttgaaaggcg gcatttctga ttcttgttgg gcctatgtag ttggagaatc tacacctgag
    35761 atcgaccgat tgatggaagt aacgaagaaa gctttgtaca tcggtattga gcaagcacaa
    35821 gtagggaatc ggattggtga catcggtcat gctatccaaa cttatgtaga aggcgaaaat
    35881 ctagcgatcg ttcgtgattt tattggtcat ggcgttggtc ctacgattca tgaagaacca
    35941 gcagttcctc attacggaga agctggaaaa gggttgcggt tgaaagaagg catggttatc
    36001 acgatcgaac ctatggttaa taccggaact tggaaaatga aaatggatcc taacggctgg
    36061 actgcgtata caagagacgg cggactttct tgccaatatg aacatacatt ggctattact
    36121 aaagatggtc caagaatttt gacatcacaa ggagaagaag gcacttattg atctatccag
    36181 tcaataggta cgggagaggt acatgaaagt ttggaacaaa ataaaacaaa atgaaaatct
    36241 gatgcgcttt attgagacga ctcaaaagcg catcactgat tcagaaatga gtacgacctc
    36301 tgtcgtcgtt gcttattatt tactcttgtc tttatttcca ttgattattg cttttggtaa
    36361 tattttaccg tttctccaca tcgatcaaga gactgtactg acctatttac gagaagtgat
    36421 tcctgagacc gtttaccagt ttatcggtcc tgcaatcaaa gaccttttga cacaaagttc
    36481 aggaggcttg ttatccgttt ctgcattagc ggctttatgg tctgcaagtc aaagtatcaa
    36541 tgctttgcaa attgcgatga ataaagcgta tggcgtagaa aatagaaaaa attttttgat
    36601 cgtccgtttc ttctcactaa ttgtcattgt cttgttcatg attgctatta gtggggtaac
    36661 tatcgttctt gggttgggac agctgatttt ggatgctatt cagccaatcg tacaaatacc
    36721 cgtgaacttt atcgatcagt tccaaacttt gaagtggcca atcacattgg tagctttatt
    36781 tgtcatcatg ttcttgattt atttaattgt ccctaatgct cagttgaagt tgaagacgat
    36841 cattccgggc acgatcttcg ctactgtcgg ctggatgctg ttatcacaag tgtttagtat
    36901 ctatgctagg tattttgcaa cgaggatcag cggttatcag atcataggaa gttttattgt
    36961 actcatgctt tggcttaatt ttgctgctac gatcatcatt ttgggcggaa tcatcaatgc
    37021 agttgttcaa gaatacgtga cgggtcatca aatcaaaaaa cgtcgagatc caagccgaaa
    37081 atggttcaat aaattgaaaa ctaaattttc tcataaaaaa aagtagaaac aagagttctt
    37141 attgtaaatt tatatcagta atttataata attatgactc ttaattaaa
//
LOCUS       DAYYCY010000009        35113 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000009 DAYYCY010000000
VERSION     DAYYCY010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35113
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.009"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(147..773)
                     /locus_tag="ACHAZB_000364"
     CDS             complement(147..773)
                     /locus_tag="ACHAZB_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8613073.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            complement(1068..1403)
                     /locus_tag="ACHAZB_000365"
     CDS             complement(1068..1403)
                     /locus_tag="ACHAZB_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFM8613074.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            complement(1390..1674)
                     /locus_tag="ACHAZB_000366"
     CDS             complement(1390..1674)
                     /locus_tag="ACHAZB_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFM8613075.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            complement(1730..3253)
                     /locus_tag="ACHAZB_000367"
     CDS             complement(1730..3253)
                     /locus_tag="ACHAZB_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFM8613076.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            complement(3246..4421)
                     /locus_tag="ACHAZB_000368"
     CDS             complement(3246..4421)
                     /locus_tag="ACHAZB_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFM8613077.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            complement(4425..4610)
                     /locus_tag="ACHAZB_000369"
     CDS             complement(4425..4610)
                     /locus_tag="ACHAZB_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8613078.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            complement(4576..6270)
                     /locus_tag="ACHAZB_000370"
     CDS             complement(4576..6270)
                     /locus_tag="ACHAZB_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFM8613079.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            complement(6267..6740)
                     /locus_tag="ACHAZB_000371"
     CDS             complement(6267..6740)
                     /locus_tag="ACHAZB_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFM8613080.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            complement(6809..6964)
                     /locus_tag="ACHAZB_000372"
     CDS             complement(6809..6964)
                     /locus_tag="ACHAZB_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HFM8613081.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            complement(7098..7478)
                     /locus_tag="ACHAZB_000373"
     CDS             complement(7098..7478)
                     /locus_tag="ACHAZB_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFM8613082.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            complement(7482..7697)
                     /locus_tag="ACHAZB_000374"
     CDS             complement(7482..7697)
                     /locus_tag="ACHAZB_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8613083.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            complement(7700..8107)
                     /locus_tag="ACHAZB_000375"
     CDS             complement(7700..8107)
                     /locus_tag="ACHAZB_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HFM8613084.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            complement(8373..9848)
                     /locus_tag="ACHAZB_000376"
     CDS             complement(8373..9848)
                     /locus_tag="ACHAZB_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapE domain-containing protein"
                     /protein_id="HFM8613085.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            complement(9838..10686)
                     /locus_tag="ACHAZB_000377"
     CDS             complement(9838..10686)
                     /locus_tag="ACHAZB_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HFM8613086.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            complement(10723..11085)
                     /locus_tag="ACHAZB_000378"
     CDS             complement(10723..11085)
                     /locus_tag="ACHAZB_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8613087.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            complement(11086..11244)
                     /locus_tag="ACHAZB_000379"
     CDS             complement(11086..11244)
                     /locus_tag="ACHAZB_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8613088.1"
                     /translation="MKRNLIIGSILIGGIFGLLPLIAKIAIFAGLALYLFTAYDEYDY
                     QLRTGGHK"
     gene            complement(11385..12009)
                     /locus_tag="ACHAZB_000380"
                     /pseudo
     CDS             complement(11385..12009)
                     /locus_tag="ACHAZB_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            complement(12006..12317)
                     /locus_tag="ACHAZB_000381"
     CDS             complement(12006..12317)
                     /locus_tag="ACHAZB_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8613089.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            complement(12361..12654)
                     /locus_tag="ACHAZB_000382"
     CDS             complement(12361..12654)
                     /locus_tag="ACHAZB_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8613090.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            12848..13495
                     /locus_tag="ACHAZB_000383"
     CDS             12848..13495
                     /locus_tag="ACHAZB_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8613091.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            13556..14701
                     /locus_tag="ACHAZB_000384"
     CDS             13556..14701
                     /locus_tag="ACHAZB_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8613092.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(14782..15087)
                     /locus_tag="ACHAZB_000385"
     CDS             complement(14782..15087)
                     /locus_tag="ACHAZB_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HFM8613093.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(15112..15543)
                     /gene="ruvX"
                     /locus_tag="ACHAZB_000386"
     CDS             complement(15112..15543)
                     /gene="ruvX"
                     /locus_tag="ACHAZB_000386"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFM8613094.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(15540..15806)
                     /locus_tag="ACHAZB_000387"
     CDS             complement(15540..15806)
                     /locus_tag="ACHAZB_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HFM8613095.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(16061..16786)
                     /locus_tag="ACHAZB_000388"
     CDS             complement(16061..16786)
                     /locus_tag="ACHAZB_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFM8613096.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(17147..17217)
                     /locus_tag="ACHAZB_000389"
     tRNA            complement(17147..17217)
                     /locus_tag="ACHAZB_000389"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17183..17185),aa:Gly,seq:ccc)
     gene            17334..18086
                     /locus_tag="ACHAZB_000390"
     CDS             17334..18086
                     /locus_tag="ACHAZB_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFM8613097.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            18102..18581
                     /locus_tag="ACHAZB_000391"
     CDS             18102..18581
                     /locus_tag="ACHAZB_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFM8613098.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(18832..20412)
                     /locus_tag="ACHAZB_000392"
     CDS             complement(18832..20412)
                     /locus_tag="ACHAZB_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFM8613099.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(20405..21148)
                     /locus_tag="ACHAZB_000393"
     CDS             complement(20405..21148)
                     /locus_tag="ACHAZB_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8613100.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(21324..22100)
                     /locus_tag="ACHAZB_000394"
     CDS             complement(21324..22100)
                     /locus_tag="ACHAZB_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFM8613101.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(22348..23184)
                     /locus_tag="ACHAZB_000395"
     CDS             complement(22348..23184)
                     /locus_tag="ACHAZB_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HFM8613102.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(23195..24124)
                     /locus_tag="ACHAZB_000396"
     CDS             complement(23195..24124)
                     /locus_tag="ACHAZB_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFM8613103.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            24316..24669
                     /locus_tag="ACHAZB_000397"
     CDS             24316..24669
                     /locus_tag="ACHAZB_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFM8613104.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(24743..25399)
                     /locus_tag="ACHAZB_000398"
     CDS             complement(24743..25399)
                     /locus_tag="ACHAZB_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFM8613105.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            25625..27559
                     /locus_tag="ACHAZB_000399"
     CDS             25625..27559
                     /locus_tag="ACHAZB_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFM8613106.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(27645..28868)
                     /locus_tag="ACHAZB_000400"
     CDS             complement(27645..28868)
                     /locus_tag="ACHAZB_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFM8613107.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(28862..30733)
                     /locus_tag="ACHAZB_000401"
     CDS             complement(28862..30733)
                     /locus_tag="ACHAZB_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HFM8613108.1"
                     /translation="MKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQIDYIHQGVG
                     YGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYWGEIHERDQR
                     MVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQFFKIIVGTII
                     QKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYGLIYSVLEPE
                     KPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAMLWADLLPFKL
                     GEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLWLNKIFLLLA
                     LPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPNFHTLTNEKY
                     LKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKTYGQFIVRNN
                     VRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLYETGFAIECS
                     PEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLMTWENTFIPG
                     LEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKNNKKRLKLC"
     gene            complement(30754..31872)
                     /locus_tag="ACHAZB_000402"
     CDS             complement(30754..31872)
                     /locus_tag="ACHAZB_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFM8613109.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(31955..32788)
                     /locus_tag="ACHAZB_000403"
     CDS             complement(31955..32788)
                     /locus_tag="ACHAZB_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8613110.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(32781..33698)
                     /locus_tag="ACHAZB_000404"
     CDS             complement(32781..33698)
                     /locus_tag="ACHAZB_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8613111.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(33925..35049)
                     /locus_tag="ACHAZB_000405"
     CDS             complement(33925..35049)
                     /locus_tag="ACHAZB_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM8613112.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
BASE COUNT        10540 a         7091 c         5735 g        11747 t
ORIGIN      
        1 gaaacccata tcaaagaact ataaaggtta tcaaaacagc gtttatgact aaatgaatta
       61 gagttatcta tcactatttt tcaagcgatt taaaccaaat ggcatacaat atggcataca
      121 aaaacttcaa taagccgtgg gggtttttaa atttcttctt gttctttaaa atcaatgagt
      181 ggagctacat aatttgaatc cactttttca aacgtcactt tttgcatcga cgaatgtttt
      241 ctttcccaag ataacgtaaa tagatttgac tctggactta taaagtctac ttcaccttct
      301 agtattactt tcttatgcaa atattcctct aataaatata gacttgctaa agcatacaac
      361 acattttcca aatttgcttc cttataattt gaagaacgac catgttttac tctgttatat
      421 attttccacc aatctggtgt atcttcaggc cacccatctc caaaaggttt tagtgaagtc
      481 aatgttaaag aattcataac cctaacacca ttatccaaac acattaattc gctagattta
      541 ataacagctt taaaatcagt tatgctttta ttgctactgc tatcaaatcc gcaaagttct
      601 ttgaaaataa tctcacactc cgaaccaatc gctagtagta gtttttgata ttcaatagag
      661 aatgttttga aattattctc atcgagcatc acaaaatctg tggtattaat aaactcattt
      721 tctaaatgta gataatactt ccaataaaaa tttgtaaact ctgctttgtc cattaaatca
      781 cctcttttga attataacaa aaaaaacacg tccacaacag acgtgttctt tgatttctac
      841 acaatcagct tgccaaaacc atccaatcag tcctagcatg cctgtattaa aattgaacgt
      901 tcatcatcta tcatctttct tagtccagca tgtttgcaac cacacctaca attttcagtc
      961 caacaataga tttcgactag ctaagtcttg caaaattcag gcagctaacc cttcatttac
     1021 taccttaatt atactaaata cccacaacgt ttacaagtgt aatcattcta agaaatctgt
     1081 ttagctatga tcgttgtata atctttaaac gccgttcctt cattgattga aataatttta
     1141 taactcttgt ttttccaagc tactttcata tcgtttgtta gttcataatc ttgatcgtat
     1201 cgaataataa acgtcaacgt tccttcaaga atggtaccta cactagcaag cacgtctttt
     1261 agcatttgtg aacgcacatt agcccaacat tcaaatacag tttcttcact atccaccatc
     1321 tgcccatctt catcttttac ccgttttgtc tttttaaact tgactctctc agtcaattca
     1381 tttacattct taatgattgg cataataagc acctctcatt tgttgtacta atgcttgtac
     1441 cgttactggt atccgttcgc tgctggcttc ttgacggttc aaataccaat gttgagtaag
     1501 taatgacacc gccaaatcaa attgctgata attatccata tctttatccg ttgcgctact
     1561 atcaatagca ctttttatat attcgcctgc tgtatcaatc aattgttgaa taaagtcatc
     1621 atcaatagtg tgatcgatcc gcatactttt ttttatgcta tccaaaatta ccattttatc
     1681 tctcctaatg aaaaaaaggc agtgggataa accgccgccc aatatttttc tatacttttg
     1741 gtgttacaac tggcgtaaac tcaatataca ctgaagcatt ttgatcaatt ttcttataat
     1801 cgttgcgaac gatcactgca agtccttgtg agtagtaatc gaatttttcc cattgagtcg
     1861 ttacttgatt tctacgagca acaaagatag attgtgcaat atcccccata atcataggga
     1921 atgttccagc tttaggattg gcaaacaatg tatctgcaat caatactact ggcataccga
     1981 ataatgattt accactaggt gctgtcacat cgggttgtaa aatgtaacgt ccatctgaat
     2041 ctttcaatgt atctaggtgg taatatccgc tttggtttag aattaccatt ttatttaatg
     2101 ctgggtctaa tgccacattg tatagttgtt tcaaatcgtc caacgtagca gccgttttct
     2161 tagtgaatgt ttttaataaa tccatgatat gcttattgtc agtgttttct accaatttcg
     2221 ctaactgatc tttgacctct tgtacaatat tcactgctga atcttccaca acctcatttg
     2281 ataaggcaat cttaccagca cgagtttcta ctttataatc aactgaagta aacatttcag
     2341 cgtcaatatc accaatttca gcaagttcag cttttgttgc taacactgct tgttggttag
     2401 ttgctactgg atatttacct tgtccatttg atactgtttt tactgttgca tattgagcta
     2461 agttataatt tgaacgtttc aaatcaaaga cttcaccgat tacttcttcg ggaacaactg
     2521 ccgctgcatt tacagtagtg actccatcac gcacttcgcc ttgtgaacgg atatattctt
     2581 catacccacg caattctgtt ttttcgttat cgattaaagt tttttccatg ttattactct
     2641 ccttttgtgc tttagaagtg gattctaagc tattgtttgt ttgttttgct tggttgttac
     2701 tcataaacga ttcgtaagaa cgcttattta cttggacatt tgatgaatca taagcgggaa
     2761 cggtcacgac actaatttca ttaagtgctt tgattttatc tattgatcgt tcaattgtgc
     2821 catcttcttt tttgtcgaat gaatctaatc caagcacgaa cccaaaggac atagaatcaa
     2881 caaccccttt tgagatattc tcatacacat catttgaata ggtcgtgtcg gttagctctg
     2941 cttcaaaatg taatcctgta tcatcgacat tcaatttcaa cgtacctgct ttgacactag
     3001 ctagtggctt tgaataatcg tgtccatata ataagaacac atttgataaa tccacctctt
     3061 ttaatgcttc gggtgtaatc acttctacaa agccgcctaa atcttttgac ggttgcccga
     3121 atttcaacgc atagcctgaa atagttttgc cgtctgttgg ttgtttttca ttttctttct
     3181 ctactttggg agaatcggct gttagctcag cttcttctgt cagccgtttt tccttttctt
     3241 cttcattatt caactggtgt aactccttcc tgttctttta attgatattt ctccaacgta
     3301 tctaaatacg taaagttcaa acttgctagt aaacgatcgc caccatccat ggggggtagc
     3361 cccattttcg accgcccctc attaatcgtg agaagtgacc ctttgacctg atccaataca
     3421 ttttttactt ttgtttcagg atcagtctct agtaactgat cggaattaaa gcggatattg
     3481 gtttcaagtt tcgtatctaa ttcactcaca aacacattaa agtaatgaat cagcgtactt
     3541 tggatatact ggagattact ttgaacggtg ctagaatgtt cattttcaac gcctaaacgc
     3601 tcaatcggta caccaaacgc cttggcaatt tgttttgtgt tccaatcatt ggaattgata
     3661 agcttcaaaa catcggtgtt tatttctaat gttttgtagt ccattgtttc atcaagaatg
     3721 atcgttctaa gagcattatc tccactactt gaaccgttgg cttcttcaaa cttttcacga
     3781 attgcgttct ttgctgatcc gtctaaatct gacttatgaa ccttcaaaat acctgaccca
     3841 ctgacaccac gagtaaagaa attgtgcaat gtgcggttgc cagcttgttg aattttcagt
     3901 tcatcacgta acgcataaag tggtggtaat cctgttaaac catcttgggt gaaatatttg
     3961 aagtgcaaaa tatcgctaga cttcacacgt ctttttttat caccaatctg ataagacaac
     4021 gtaccattat ctaattgagt aaccgttaca cttgagttag gtaataagtg aatttcttct
     4081 accttttcac cgttgcgttt aatctctgca aagctattac cgtttagcaa catattgacc
     4141 gccaaagcaa actttaaatg ccaaccatcc atttctgaat taggtttctc gtttagtaac
     4201 ttcaccagtt cattatcagc ttgtggcata ttgtttttga ccaattgaat cggacttgaa
     4261 gcaatatcac tagcaataat tcgaacggct gcaaacacat cactatttct taatgcacga
     4321 acgcttgtaa agctggtggt gtaatcatcc gattggattg ataccacatg ttgtaggaaa
     4381 ggatctcccg ttgtattttg tctcgcttta aaaaatgcca tcttttaacc tcccttctgt
     4441 tctgtattta gcatgaccgc taccccaata agcactatgc ccagcgctaa caagccatag
     4501 tagacatttg ttagtaaggt gatagcaacc aacacacaca ccaatccaag aagtaaaaga
     4561 acggtatgaa tgtaattaga aagaaaattc ttcacttgta taaaattcat tgtctgcttt
     4621 cgctccttcc gttgtggtga agtaatccat tgcaaacaca taggcattga ttaatgcagc
     4681 tattggatca attttgttac tgttctttga tttattaatc tgaatgccgt tgttatccgt
     4741 ttttaaaata gcgttgttca ctgcatgggt gagaatcgta tttttattgt gaataacgtt
     4801 gccttcataa acttgttctc taaaagttcg ggtcggaacg tttagagtaa tcgttccttg
     4861 tcttacttct aacattgggt agttggcttt ttcagcttta ctgataagcg aattagcgtt
     4921 atacgggtca taacaaatcc ccatacattc caaatcattt tcttgaatca aatcttgaat
     4981 aaactgaaat acttggtcat agtccacgat tccactttct aattgagtaa tggaacactc
     5041 accagctttt tctagttctc gataaggcaa accgtcgcgc ttttctttgt cttgaagccc
     5101 atatttggtg gctacaaagc tatgtgaatc acaataaagt tcgccgttat ctagcggtac
     5161 gatccatgaa acgctagtta aatcatctgt tttagataaa tccactccga tataaacggg
     5221 cttgcctgta atatcttgtg gttctacttc ggttgcctgc cagtcttcag caatcatata
     5281 gctgtcctca ctcgcttgtc tccataaatt gaagtttttc accaatacag aattaaggtt
     5341 attttgtttc aaagcaaggt tcacatcatc ttgaatggtt ggaatcatta cttttttgat
     5401 ttcttcactt tcaaaaatcg gattggcttt gatccatttc tcttgatcgt gaatttcttc
     5461 gggatcgtcc aattcccata tcgctataaa gtacctgtcg gcttgttctt cgccttttag
     5521 cacacgatct aacaataagt attcctcgta cataggaacg tttaaatcaa gcccagcggt
     5581 actaataaca cacaataagc cgttcttttg ttgggtcatt cctgacttaa tgacgttgta
     5641 cgttttacga gtttttgctt cgtgccactc gtcaagaatt gcggttgttg gtgcaaaccc
     5701 atctagcgtg ctggtttcac tagccagtgc catagcgaac gaattagagg gcaagtgctg
     5761 aatcttagag ttcataattt tcagttgtgg tcttaaaaac ttactagact tgaccacgtt
     5821 tctaagtgaa ttagacagca tatcatagcc caatttagct tgtttcaaag cgttacttac
     5881 aaacaatact tgccttgctt ctgctggttc ttgttccata atcaacgcat tggcagccat
     5941 gccacttgcg atatacgttt tcccattctt acggctcata ctggtaaacg ctcgattgaa
     6001 tcgcctgtaa ttcccttctt tagtacgcca gccataaaga ctgcctagaa tgaatttttg
     6061 aaatcctaac atcttgattg ctttaccgtc cgtagatggt agcaactcaa tgaatttaat
     6121 cgctttggtg gcttgttctt catcgaacac ataagggaag ctatctgact tagaacgctc
     6181 caaatcgctt atatgacgct cacacgcttg gataattttc ttgggtgcaa tcagcttacc
     6241 tgataggact ttctcaatgt aatcattcat gaattcatca actctttgaa cgggtcttcg
     6301 ggttcatcat ttttaacctt gttcaaagct agttttgatc gtgattctaa ggtcatacct
     6361 agtgaggtgg cttgtttttt caaatcactc atagcttgtg attgaacccg aaccgctgga
     6421 ttggctttct ttactccgtt ttcgcatgtg ataaacgtac ctgattttct tatctcggct
     6481 tcggctgttt tcattcgagc aaaggctaaa caataggcta ccaatgaacc ataatctgct
     6541 tcactcaatg gataatcttt ttttaatgtt ggtacgatac gattccattc acttctagcg
     6601 ctcgctgcca tccagtcagg gggcgtgata tttattaatg gttgctgctt gaacaattct
     6661 tctctagcgt ccacacggat tgctttttct tcacttgata tattgccttt tgtatcgtgt
     6721 aatattttag ggtgtcccat gttgtcctcc tttcagacaa aagaaaaagg aacgtaagca
     6781 attaagctca cattccttcg tttttcgatc agaatttttc tttatctaat ttatctatat
     6841 aaatgatttt tccgttctgc atttgaatgg tcacttgacc aaaatgaggt aactcaatgg
     6901 tgcgctgttt ccctgtttta tcagtaataa caattgtatt tttcaataaa tctcctactt
     6961 tcattatata ctttttcact tgtgattaca agtgtaatcg ttatttgtaa ttacccttat
     7021 gccttttcac aacggttatt tgtaccaaaa tgagtaggca tcgattgtca gtaagtttca
     7081 cactatgccc ccttaactca aagggtactc ttttctcgtc tttgtctttc attggctgtc
     7141 ttggaattat ggtgtgcatg gcataaagaa cgaaaattag tttcatctaa ccgtctattc
     7201 caatcatctt ttagttcaat tttgtgatct acaacatcag ctttccttat caagccttct
     7261 tctaggcaat cctcacatac tggatgattt aaccgataca agtatgaagc ctttcgccat
     7321 gttctacttt gatagaattg aaaatattta ccttcgcttt gctttctttc ttcacgctta
     7381 attgccgttt gctttgcttt atgcttccca caatacttct gcctataatc aatcaatgtt
     7441 ttacatccag catgattaca ttgtttttta actgccatag attaccaacc aaagcgatct
     7501 cgttcgtcag aatcatcttg ttcccatttt gatttgtatt caatataagg cgaaactatc
     7561 ttcaagcacg ttccgtcttt aaaattaact ttagtgattt tatcttcctc aaaactaata
     7621 aagtctacat cttcttgagt gtttgaatac tcaatgtgtt tctcaccatc gaagtatcta
     7681 atactttcca tatgcataat tattctacct ccaataaatt agttatcaat tcatcatctg
     7741 ttagtagcaa agccaagaac ttatcatgga tgtttaaaaa caaattatcc tcattcgatg
     7801 taagctctcg ttgtctcgct cgatcatata ggttaagcag acaattaaat tcatccatta
     7861 gctcacctgt atcatcatag ttgccactat ctaccaatga tcccactact agcgattgac
     7921 accacgctac tgactcctgc agccaatcag gttcttcttc cattatcttt aatacttctg
     7981 gatcaaatgt ggaataatct tctgttacca atcccacact aaatgccagc gtgttcatta
     8041 gaaaccgatc catttcatct gtatattgaa agttcggtgc tttgtttgtt gaaataatat
     8101 tgagcattta ctttcctcct tgattcgtaa cacgtggtaa cacctacgta acaggtaccc
     8161 gttaccactc aatcccaacg gggacaaggg ttctagcgtt ttggtaacag gtaacgggta
     8221 aaatccagta acattattcc ccagcatatt agctaactat ttcaaaagtt ttaaggaaat
     8281 aaggtgttac cccgttacca aattgctgta tcccttgggg cagtaagggt aaactagtaa
     8341 cgggctaggt gttactgacc cgttactacc cgttactttt tgtactttcc agctcgattt
     8401 ttattttcat aggcatttga cttattgtaa aatcctcgtc gtttatttct ttttggcagt
     8461 tgcttccgtt cccaaccagg aatactgcta ataatcaagg caatcttttt agtttctgca
     8521 tctgatcgac cattcaataa atcctttgct tgtttatcaa aaacagcttc caaaatatca
     8581 gcggtgagta cgctatcaac taaataaaac tcttgttgca tagtagctcc attatagaaa
     8641 tgctgtaaat ttttctcatt acatttactg tcaggatagt tctgataata agaacgtctc
     8701 gcccacgctg gagcttcttc ccatttatac gggatttcca tttccaaata tttagtgatt
     8761 gcttctttaa ttggattctc catcttggca tcctcttgat agtcttttgc aatttctaag
     8821 acttctttat cttcatcggg actaaaatag attcgttgtc ctttatcaaa aagcaccttt
     8881 gcttcagcta acacttgaag aaaatattca tcctttgttt taaacggatt catctttggc
     8941 ttattcttac aaggcaaagg ataaaaacgg cggtttcctg tatcatcttt caaatactgc
     9001 ccatcattcg acgttccaat aaatacacat tgccttgccc aagctatgac attccgacca
     9061 taaggaggtc taattttatc gatcttggca ctgatgaagt tttttatctg ttcaatttta
     9121 gttttgttca ttgaagataa ttctccaagt tcaataatta cattgccttg aagctgcata
     9181 taatcatctt tcttttcacc tagcgaatct aatgaatcgg tgaaaaaatc atccgtgtaa
     9241 agtaatgctg gaacggtact cttaccaatc ccttgcggtc catccaaaac tggaacgatc
     9301 tcaaacttta ttcctggttt atagatacgt gcgactgatc cgactatcca acgttttgtt
     9361 acttctcgtg tgtaaaggtt gtcgtctacc cctaaaaggt caataaacag tgtttccagt
     9421 ctaggctgtt gatcccattt aactttttcg atacgttctt ttactgggtt gaaaacattc
     9481 tttctagcca catttactac tgctttgtgt attttgttgt catcaaaaac tacattgtag
     9541 ttttcttcaa tatatgccgt taaggcatcc tcaaaactgt cttcccattc tccagcggta
     9601 cgattgatcc aaggagaatc ttttaatagg tgaatgtatc cgctaaattc gttataagca
     9661 attgttccag ctaaattgct atcattggtt aaaatcaatt ctgcattcac aacactgtta
     9721 cttttaatct gatcgtcttt attgcatctt aatcgtgaca tccaggtttt caattcaatg
     9781 atattgtctt cacgctcgct ggcttcggga aagtctaaaa tactatctaa tttatgctta
     9841 tgagccatga gctttccccc tcaaatcttt tttaatcatc gatcgaataa tttgattcac
     9901 ttctttatct tttagcggtg gttcattgta cgcatttgct gtataaatca attctgctaa
     9961 agtttgatta tctacaccgt gccatagtaa ccaaccgcat aacttagtta aatgatcgtt
    10021 tctactacct tcgccagcgc cttgaacaat catatctaat ttttttgctg tccctgtttt
    10081 tgctacgggt cggtattgag tctttatttc ttgcggtttt tttagcagta ggttcactag
    10141 ccaatcggga gctggtgcaa tattatttag agatacgttt tcaataggtt catatgctct
    10201 cccaacactt cttgaaataa tcatagttgg cgctattgga actcctttag tgataatatc
    10261 aatcccgctt aaacttttac ctttatcatc acgcccgaag atatcagcaa atgcacctgt
    10321 ttcttgttgg ataattacac catcaggaat tttaaagaag aaatggattc ctttacctgg
    10381 tgtgatttcc gtataagtag aaggaaatgg atcatacatt gaagcaagtt ttttataatt
    10441 ctcaacgccg tttcgtgttt cagaatgttg atctaggtca actaccacca tttgatgatc
    10501 ttcaagcatt aacccaatat ttctagttgc atcttccgtc caccattcat taatttgatt
    10561 agaatcgtta gttgcatcat tccaaccact agagcctttt attggaattt tagttccagg
    10621 agataaggga taaattgacc atcccgactt ttgtaattcc gttgctgctt gataattatt
    10681 tgccaataaa cctctcctcc tttttcaaaa tactggtata aatcattcag acacctccat
    10741 gagcttcaac tgctcaagtt cgtatttcaa cccatcccga acaaattcag caccttttaa
    10801 ttgattctga tactttaccc atgtattaaa tgttttcacc cttgcttctt gcatatcagt
    10861 aatgtttaac catgcggtta aaatctgatt ttttagctcg ttgatttctc gacaaattat
    10921 tttgtgttct gcatgttttt tgagtacata caaaactgtt agtttttcat tgaatgaatt
    10981 tccaaagtag gttttcccga tattattttc tagttcagcg atcccttgat taatttcctc
    11041 taattcaagg ctatcgagtt ggtttaatag ttttgttttg tacatctact tatgccctcc
    11101 agttcttaat tgatagtcgt actcgtcata agcagtgaag agatataacg ctaatcctgc
    11161 aaagatagct atttttgcaa ttaatggtaa taagccgaaa attccgccga ttaaaatgct
    11221 tcctataatt aagtttcgtt tcataatggt tccctccgat tttttaatat ggtataatcg
    11281 gaacaaagaa tatcgggttt tatcccaatt aagttgcctt tatcctgtcc gccaagactg
    11341 ttatggataa ggctttttct ttgttctcga tcatgttaat gctcctaagt tgtctgcaat
    11401 tcagcagaac gattataaat tttctcactc actgaaaact gcaacccata tttttcttta
    11461 acagtcatta attctactgt ctcatctaaa ataggttcac gatcaaccaa catttcagga
    11521 gtcatatctt tcttttttac cattttggaa tgaccataaa tagttgatac agctttattg
    11581 gctatcacat tcgctttaat gtagtctact ggttttggtt cagtaatagc gtggcttaat
    11641 ttagtcattg cttctttttg gtgttctttg tctagcatgc ggaatacttg aaagccttca
    11701 agtcctgtgg actggcgtag ttctttgatg atgtcgaaaa tccagtcttg aaatgcctcg
    11761 gcttcttttt tacggctttt gaatgccatg cggtaaatat tttttttcgt tgatgatgtt
    11821 tacatcttgt gttctgccca atgaatcgat gaccttacta gtagtaaccc catctttagg
    11881 caaagagtga atggctctag taacctgttt aagacctaac gcttccgcaa catctttcgc
    11941 cactgcccac cattcatcat taatatcgac aaatcttata atgtgtccgt tccaaatttc
    12001 agttttcatt caacgtcctc cagttcaagc cctagcaagc tcgctaacaa ctcaacttgc
    12061 tgatacaaaa tttcttgata ctcttcaata gaaagtcgtt ctttaaactc tgcttgtact
    12121 aaatcgttca agccacgcaa tactccgctt accgttggtt cttgtggtag caatttttga
    12181 tagtccattt gttcgtacaa acattcaaac gtttcttcta gcgctttgcc ttccaagttt
    12241 gctaattctg ccgctgtgtc ttctgtaaga attgttgata atactttttc gattgcttta
    12301 ttttgtgtcg attccattat tatttcctcc tattaatccc tttgccccca cggcttgcgt
    12361 ttatttttgg tattcttcca taaagcgatc acattcatct tgtgaaattc ttacgactcc
    12421 atcaactata atcaacttta aaccgttagg aacatatttc tgcatcatag tattgtatga
    12481 aacgttcata tacttagcag cttgtgcctt gttcatatat tttggaaact ggtacttttt
    12541 ttctaataat gaaagtaacg ttccttgagt ttgctctact tgttgaacaa ctcgatcgtc
    12601 aataatttta aaagctggat catcaagcgt gagtttgtca atttttaatt ccatatttat
    12661 cccccctcgt tagcatacat ttctatacaa agattttaaa aaaatgcaag aggctcttga
    12721 atacatttct atacctctta taaactcaat ataccatcat accttttcat ttgcaagaat
    12781 tttaacatac ttttttgaac ttttatgttt cacacatatt taaatcgtga tatagtagag
    12841 aaaatatatg aatggtggtg aaaatgtggc tagaaaaaaa taccccgaag cagtggagac
    12901 aggacaaaga attagactgg ctagaattga aaagggactc tcaatggaac aacttggagg
    12961 taaattatcc ccaccggcca gtaaaggtgc cgttagtaat tgggaaaatg gctataatct
    13021 tcccaataac accaggctta aacaactttc aaaggtatta gatgtatcca ctacctatct
    13081 attaaacgga tcttatacat taagagatat gcatatgatg cccgaaatta agcgaaatgt
    13141 gcttgaaaag caattgaaaa aaactattga aaaaaataac tctataattg attctaatct
    13201 ttctaatgct atcaataaca tcgaagaaga taacctgact gtctcacaga agatttattt
    13261 aacaaacgca ataaaatatc ttaaaacagg atctcgaatt gaatttcctg acgggagtac
    13321 cccaattgct tatctcgcag ctcttctaaa tacgctttta cgatatgact cattaattaa
    13381 tgattcagaa aaaaatacag atgagaaaaa agagtatcaa gaattttatg aaaatgatct
    13441 tctaaaagag ttcacagctc ttgttactga gctaaataaa cactattcaa aataataata
    13501 aactgcctcc tccaaccgcc cccacggctt gctacactat ggagggaaaa ctattatggc
    13561 aacattcaag caatatgaaa ctaaaaaagg taaattttgg ctctatgaag cctatcttgg
    13621 aatgaacaaa atgactggca aacctgataa ggttcgtaga cgaggttaca aaacaaaaaa
    13681 agaagcccag cttgccttgt cacgcttgca agtggactac gataaaaacg gtattaaaaa
    13741 agcgaacaac gaaacgttta aagaagttta tgacttgtgg ctagaaacat ataaaaccac
    13801 tgtccgagaa gccaccttca tgaaaactga aatcaagttt agaaagtgga ttcttccaaa
    13861 atatggaaat cttcgtgtga atgaagtcac ggtaaaaaaa gcccaacaaa ttgtaaatag
    13921 ctgggctgaa actactgacc aatacaaggt acttcattca acttctaagc gtatttttag
    13981 ctacgctatc aatcttggga ttattgagac taatccacta gacaacgtat taatgcctaa
    14041 aagaaccacc actaagcata aagaaaaggt caaagtttac agtaaagaac aactgatcac
    14101 cttgtttgat tacctaaaca gcaaacctga aacttatcga aacgactttg ataaaacgtt
    14161 actacgcttt ctcttttata gcggggttag gatcagtgaa gcccttgcat tgaattggtc
    14221 agacattgat tttcaagaaa atacggtcac catcaataaa acactaagcc aatccaaaaa
    14281 tggctataaa atcagtgatc ctaaaactga ttgtagtgct ggggttcttc cactagatga
    14341 aagaacaatt tctatattaa aaaaatggca gattaatcag cggaaatata tgctgacact
    14401 tggtgtaact gatccgacta tgattttttg tggtatctat aaagaactaa tcacacacca
    14461 cgctatttat gcaagattga ttactatttg tgaaaaagct atggttccat ttctcggtgt
    14521 gcatgtagca agacacactc atgcttcaat gctcttggac gctggagcta ctatggtaga
    14581 agttcaaact agattacggc atacaaaaat tagtcagacg atcgattgtt acggtcactt
    14641 ggcaaaagaa acgaaagaaa agactgttga aaaattagta caacacttaa atattgacta
    14701 gatggcatac aatatggcat acagtaaaaa ataaaaaaag gagaaaccct atcatatcaa
    14761 ggtttctcct agctttatag tctattcttc ttcttccgcc atgaatgtat tgaatacttc
    14821 ttcaatcatg tcccattctg cgtctgtttc gatttgttct aactcgcctt ctgttccatc
    14881 ttcgttttcg atgtatgcat aagcttgtaa ttctacatct tcatcttctg aaacaccagc
    14941 aggatataaa agaacatagt tacgtccaaa ttcttcttgt ccgtcaactg ttagtaggat
    15001 ttcatataat gtttcatttc cttggtcatc caccaatgtg atgtgttcgt gtccttcatg
    15061 gtcgtgggaa tggttatgtt ctgtcataag attacctctt ttcttttatt attaaaataa
    15121 ttgcttacct gagccgtcta aataattttg taagatcatc acagctgcta atttatcgat
    15181 gacttttttt cgtttggctc ttgaagtatt tgcctgctct actagcatac gttctgcctg
    15241 aacagtggtt aatcgttcat cttgataatc aactggcaag gagaatagtt ctttgatctt
    15301 ttctccgtat gacatggaag cttctgccct tggtccgatg gaattgttca tatttttagg
    15361 taatccaact acaaaccggt ctacttcata ttgtgctact aattccttca accgatcaaa
    15421 gccaaactct tcttctgctt cattgatccg aatgatctct actccttgag ctgtccagcc
    15481 aaaggggtca ctgatagcaa cacctactgt cttagaaccg acatccaagc ccattactct
    15541 cataaattca ctccatgatt tgagaggtag tccttgacaa ttacttccat gatctcatcg
    15601 cgttcgtgac ggcgaatcaa atttcgagca tcacgataac gaggaatgta ggccgggtct
    15661 ccagaaagca agtagccgac aatctgattg attggattgt agcctttttc ttccaaagcc
    15721 cgataaacaa tcgctaatgt ttcgctcact tcttttttgc gactatcgtc aaaaccaaaa
    15781 cgtacggttt catctgtaaa acccatgatt ctacacctct ttcccgcaag gtaattccag
    15841 cttaccttct acaaccttat tttactcgaa gaaagggaaa actacaaaca aatattccaa
    15901 acttccgaat aaattaagaa acactatttg tatgtttttt tccttttttc tgtagcttta
    15961 tcaaaagtac tcttttcata aagtatacgc tatatttctt tgaatgtatg tttttacaca
    16021 attttttcat aatattcata aaaaattcga atagctgcta ctaatttttt gtttttgttg
    16081 cttgtcctgt ctcataattg atcgtgtagc catcttgaac attgtagata aagacattga
    16141 attcaatttt tttgtcttca atgctttctg cttccaattg cactcccctg gctaccagtt
    16201 cattcccctc aaaatgagga gtgacacggt agcgtacatg atgatttgtt tctttgatat
    16261 atgccgcaat atcattttcg tgagcaagca tgtatggagc attcaacgaa cgtgtccctg
    16321 tcatcaagtt tttaatgttg ttattttgac cagtcagctg gaaaccaatt aaatggctgc
    16381 gattatataa ccattgcccg tcacttaatt tcttttgttt ccaacctgtc gggtcaatat
    16441 acaaaggctc tctcttttca gtagggaaca aatcttttcc aagcatagca ttggcttgtc
    16501 ctactctgtt caatttatca atatttgaat agctttccca tgttcctttg gaaaggctaa
    16561 gatcctcttt tgtaaattcc ggctgattcc cattgatttc tatttcttga tgttttccat
    16621 catattctgg cacatccgct acttgagaag cttgtggtgt ggaatcaaaa atagttgtaa
    16681 tctgctgaaa gatatctgaa tctttttgtg tggtggaaac accgaatgcc cctgcaatca
    16741 cagcagcgac gatccccacg atcgccttgg ttgttttttt gctcatttct ttactccttt
    16801 actaacagct ccctaaatct taacatatgt tcccctataa ataaatacta gaaagaaata
    16861 gaaacagcaa aaaataaaac ctgttttaag atggcttaaa aatacaaaaa agagacggat
    16921 tcctcgtctc taatcagtca ccactctatc tgtaaggtat ttattattat tcaaaaattt
    16981 tcttcatact caagacaata cactgatttc acaaccgctt ggtaaacggt gcgcaaaagc
    17041 ttgcatctgc ggtaataagt ccatccaagt taaaaacata agaagttgtt tttgcttggc
    17101 tgttcttatt acttgttgca gagcagcttt ttcacaacct attttaagcg ggtgacggga
    17161 atcgaacccg cgacatcagc ttgggaagct gggattttac cactaaacta cacccgcaat
    17221 acaaaaacag tatacgcctc ttttattcct tcctcaagtt tttttattct ttacgcttct
    17281 ggtatgtttt gtagtaaaat aaaactaatc aatcaattat tggagaagat gctatgacgg
    17341 gaaaagaatt agatctgttg ctggacactt gtttactcgc tgggaaaatc atgatggaaa
    17401 gcaatgcgga gatgtatcgg gtagaagata cgatgagtcg aattgcttta gcttctggaa
    17461 atttccgttt ggtcagctat gtcactcaaa ccggtctctt tattggtctt gatcgtacct
    17521 cgaccatccg aatggaacaa attaccaacc gttcgattaa tttagaaaaa gtagtaaaag
    17581 taaatgatct ttcacgtaaa tatgttgctt cagagctaac attagaagaa ctttactgcg
    17641 aactgcaagc aatcgaccga gaccgttctt tctttcctgt ctggttgcaa atcgttagcg
    17701 cggcagtgat cagtggaaca attatggtgt tatttggcgg aaagatgacc gatttacctg
    17761 tgactttagt cattggagga ttgggatacg cagcttatct atatagtttg aaatttttta
    17821 gaatcaaatt tttatcggaa tttttatctt ctttattgat tggcatagcc gctatctaca
    17881 gcgtacgttt tggcttagga agcaatcagg atctaatcat tatcggatgt gtgatgcctt
    17941 tagttcctgg agttcaaatt acgaacgcta tccgtgattt gctagctgga cattatgtct
    18001 caggagtttc tcgcggaaca gaagcgatga tgactgctac gatgatcggt tttgcgattg
    18061 cttttatttt ccagcttttt tattaggaaa ggaacacaag tatggggacg atatttattc
    18121 agttttcttt tagtttttta gcgactgcgg catttgccgt tattacaaat gtacctaggc
    18181 gttcattgat ttgttgtggt ctttctggtg cttttggctg gatgatctac tggatctgtg
    18241 ttcaaatagg cggatctgct gcttttggtt cattgcttgg ttctttagga gtagcattca
    18301 tcagcgatct tttctctaaa cgtttgaaaa tgcccgtaac gattttcaat attcccggga
    18361 tggttcctct agtccctggt ggtttagctt atcaagcggt tcgaaatctc gttactgaat
    18421 cctatcgaga agcggctcat tatgcggtcc aagcaattat gattgctgga gcaatcgctt
    18481 taggtcttgt attatcagaa gtctttaacc ataacatccg taatttcaaa gtaaagcgtg
    18541 acgcaatcat gatgaaacga aaaaaacggc tttttaaata aacgaagtgt aacaaagaaa
    18601 aggttgtgaa agagacgttc agccttgagc gatgacaaaa tatctcacaa acattgcgac
    18661 aagtaaccgc aggagcaata agaagtaatt atgagtatta aaaaaagttt tgtaaaaata
    18721 gcttctcaca tttttacata aaattgactt aatactgaga aactgacttt tgaacaccct
    18781 ctatctgtaa aaaaggggct gcgacatgat ttttgtcacg gcccctcttc tttattcaaa
    18841 ttttttccaa aacgttcgtt gataataaac gatccataga gcggaaagta ttagaactaa
    18901 accgatcaca ggaatattca ctaaccaaaa tggataaaac aaagccaaca ttccgtataa
    18961 gacgataaga ataatactga tgatcaatga acccatcata gttgcgacta aacccaaact
    19021 tcctgaacct ctgttaaata attggttaat attggtccag ttcagcagca gcaaccgata
    19081 atctctagcg aaataacgta gtgacaataa gtagcagccg aataaagaac caaatagcaa
    19141 gctagggata aaaaacaaag gcagctgaaa agacagtccg cttaataatg cgatcccacc
    19201 agttaaaaaa ctctgcaata tccatccgaa tctgaacttt tcttttaaat attggttcat
    19261 agagattggc aaagaacgga taaacagaaa attttcttga tccaaagaaa taagattgct
    19321 gataaaagaa gtctgattaa ctgttaagaa agccaatgcg atccctacca ggaaaacaac
    19381 accgattaac ttattatcta aatcagttaa ttttatttcg cctcctaggg caaaagcaag
    19441 aataaaaatg attggagtta acaaggaaga agaaagaact tgcataatca aattaggttc
    19501 ttttactaat tgagcattgt aactaaataa aagctgatgc acattttggt tctttttatg
    19561 ggttcttttt attttgaact cactgtttga atcagtcagt tgctctgtaa gctttggtac
    19621 tatcagctgt tttacagcaa ggaaaaatcc cagagccgtg atagctagta ttccccaacc
    19681 caataaacct tgttgagaaa gtggctgact ggcagcatca tagaatggca ataatatcga
    19741 aatcggatgc cgatctgtta actcattttc aatagtcccc gtttgatgat tcatccacat
    19801 gattccagca aatgcgatca gcatcgagct gccaagcaag atactagaaa ctgtttttct
    19861 atgtttttta aacactgctg ttcgagccaa accaaaaaca atcaaactag ccagacaaaa
    19921 aagaataatc aaaaatagag aaaacaaaag gattgagata atgatagtaa gcaaaatccc
    19981 tagatcagct cgccatccgg ttaaaataaa catgactaga agcggaaaaa caaaaggaat
    20041 gatggtcaaa gcaactacca aaatctttgc tgaaaaaacc tgttcttgtc caaaaggcag
    20101 aggcaaaaat acttgcaggt catttccttc aaaaaagata ttataaatga ctgatatact
    20161 ttgagttaaa cctaacatac caaaaagtgc cacgtaataa gtaaagaaac ctggcaattt
    20221 aacaaagttc atagcaaaca tagcaaatcc gtaaatcagt aaaaaaatca gccctgaaaa
    20281 aagattttga gacaaaagat agatagttaa tcgctttccc ttttttcctt tctctcttgc
    20341 tttattcgtg agctgtggat taacatataa aagattaact cgaattaatt ctaataattg
    20401 tcgcttatac atacggtcca tctcctgtcg cttgccttcc agccattttc aaataaatct
    20461 tttctagcgt ttcatcaggt gagccattca ataattcact aactgccccc tgataaatca
    20521 attcaccttt cttcaaaata gccagtttat cgcataactg ttgtgcggta tccaatacat
    20581 gtgtagaaaa aatgactgtt tttccttttg cggcatgagc cttcatcatt tctttcaaat
    20641 caaatgctgc ttgaggatca agtccttgca aaggttcatc caatacccaa atatctggat
    20701 ctggcagcaa cgtgccgatc aaaatcgttt tttgacgcat tccatgtgaa aaacttgcga
    20761 ttgtttcatt ttgatgacta tacatatcaa aaagcgctgt caattctgct aaacgctttt
    20821 cttttttttg tgtgtctaat tcataggcag ctgaaatcaa gtcccaatat tctccagccg
    20881 tcaattgtaa aaaaatatct ggggaatcag gcacataacc gattttttgt ttgatactca
    20941 atcgattttc cgtcaatttc attccatcta ctaaaatcgt tcccgatgat ggttcaataa
    21001 tactaactaa acttttaatc gtagttgatt ttccggcacc attatgtcct aaaaatccaa
    21061 aaatttcccc ttgtcttatc gagagagaca agtcttttaa tgcttgtttt ttcccgtaac
    21121 ttttacttac attgatgaat tcaatcattt ctgtactcct tttgtttgtt attacttttt
    21181 attgtaccct tcgttattgg tggagtaaat gtcaaaaaga gtgcataaca aaaaaggata
    21241 gaaacgacta ggctgctact tgggttgttc tcttctacag gaaaacactc ttctaacagg
    21301 taagatatga gtctttgttt tctttaaaat gattcatttt ctatcttttc ttgcataaaa
    21361 ttctttagtt tatcaatact tctctttccc atctctagtt gttctccatt tgcaaatgtt
    21421 actatttcgt ttactctgct aatagatgta atgttttcag tattaatgat ataagctttt
    21481 aaatctttac ataaatactc atattcgagt gattttttat attttttaat tgtgccgttt
    21541 acgagaatct cttcatcttt caatactagg cctattttat ttttataacc tggcatgctc
    21601 attagataaa ggatttcatc gtaatggata tagtgatcgc tattaccata acggacagtt
    21661 gtccaccttt ttttcgtagc atgttgaaca ggatagtttt ctttttcttt caataagtaa
    21721 ctttggatgc tttttttatt tttttcataa gtgaattctt ttatcaaata accactaggc
    21781 gtaatctttc gattaataac agttattgct ttattcggat gagacgttag ataaacaatt
    21841 ttacactttt cattcttttc tctaagcatt tcgcctagtt cgataccgct ataataggcg
    21901 ttcaaatcga tgtctaggaa aaaaatatct gaggaataga tttcattatt tttaatgaat
    21961 tcaacaaatt gtataacgtt caatccctga acgatctcaa atttccaagg acttaataaa
    22021 attctggatt cttccaaaat ttctactagc attttttcaa aaaccaaact atcatccaaa
    22081 ataaatatac gcgttaccat aaaaagtacc tccactcgat attttactat caaaatcatt
    22141 tcatacaact tttcaacata catgttagga ataaattgta attctcttca caatcttttc
    22201 caacaccttt gaaaccgata tcaaatactt tcaggatgct gcttctaaaa taaatatcaa
    22261 acaaatgaat acatcatgca aaaaaagcca gacaaccgac gtccttgcgt gtcagctgtc
    22321 tgagctatgt ttatttattt tgttctttta aaaagaacga tacacatact gatttttcgg
    22381 agcagagact ttctcagcgt ttgtaacaac tacagatggg atttcacgct tgaatggttg
    22441 ataatagtct gattggatgg ttccttctat tttataccat tcatcatttt taaccgttac
    22501 tccttccggc atatgcgtca acaaaccaaa gactcctgaa tctgccacac aatggataat
    22561 gccgaatcgg aacaagaaga tatctgtatt gctatcgttc ggtgtttggt aaacaaatcc
    22621 ttcatagctg atttttttgc caatgaattc gctagggtaa ttataaatta gctccataac
    22681 ttctaaatag ttttcatctg tgatcttgat agtttgctta tccccatact tttctttcaa
    22741 ctcattcatt tggtctaagt agtctgattt gttgaaataa atactggtat ccggctttaa
    22801 gtattgtgtc tgcatctccg gatcccctac tgattcttta cttactggga aattaaatcc
    22861 tttggcttct acgatcgtag tgtcgagact aactgttgga aacaacgtcc ccacgattac
    22921 tggcaaagct agcagtacat aagcgattcc acgttgataa ggttttgaca acccatggtc
    22981 atgatcgtgc tcgtgtgcct tttcttctgc ttttccgttt ttgacccaca aatacagctg
    23041 aactaaagcc aaaagagcag ataaaatcat tgaaagaata gctaagtagc gataatggac
    23101 attgatatat tgatcgagtt tgccagaaac ttgcaagtac atcatcaatt cgaaataccc
    23161 agataaaatc aaaaaccgca gcatattttc cctcctaaat aaataatgtg aatccaataa
    23221 cgctaagggc aatcagtccg atcagcgata aaataaagcg tcctgtaaaa tatcgtttca
    23281 tcatcagtag atttttgata tcgaccattg ggccaaagac caaaaaggca gctacaggtg
    23341 ctgtgccgaa cagacttaac aaagaagatc ctataaacgc atctgcttct gagcaaagag
    23401 acatcgtaaa ggccaataac agcatgatga taatggctaa aatctttgtt gagccgagcg
    23461 ttagcataat tctagttggt aaataagtct gcatcgcagc tgcgattaat ccgccaatga
    23521 tcaaataacg acctgtatca aaaaattcgt cgattgaatg tgtcaacaca tgcccagttt
    23581 gatggatcag ctgatttttt ggttcattct tctcactttg ttcttcttca tggacatgat
    23641 gatggtgatt ttctgctact ttagacgtga ttttttcttt caatacagcg tctttttgaa
    23701 aataggcaag ccagacacca atcacaaggg caacaaaaaa gctgcctatc acccgccagc
    23761 ttgccatctg ccaagaattg ccaaatgcga taaaagtgga aaaaatcacg attggattga
    23821 tgatcggagc agtcagcata aaagcaaacg ctgtgtgaac tggtacgcct tttttaacaa
    23881 attgatgaac gatcggtacg atgccgcatt cacatgaagg aaagaaaaat cctaagatcg
    23941 aaccagtaaa aatagagaag atacgatttt caggaagcca tctcttcaca cgttctgggg
    24001 tcaaaaatac ttgtaaggcg cctgagataa gacagcctaa taatacgaaa ggtaatgctt
    24061 caataacgat cgataagaaa atcgtactca tctgcaaaac cgaatttgga ataaaagaaa
    24121 acatgagttt cctccttttt atttctagtt cattcgcttt gcaaatatac gcttttattc
    24181 aaaaaaaaca agttgctctg taaattttta cacaactctt attttttacc aaagcacttc
    24241 aattgacttt ttctgttatt cccattaaat taaggtctca tacatatcac ttctatcata
    24301 aaagggagaa taaaaatgaa acaaaccact aacttgactg aagttcaaga catactgcaa
    24361 cagtcatccg ttgccgtcat ttatctttcg atgcctaatt gcagtgtttg tcacgctgtt
    24421 cgtccacgac tagaggaact tctcacacat tatgatattc cggcacttca tttggatgca
    24481 tttgaaacac cagaagtagc tagtaaattt gaagtgctga ctgcgccagt tgtcctgatt
    24541 tttcatcaag gaaaagaagt ctttcggcaa gcacggttca tcgactttaa aaaaatccgt
    24601 tatctactag atgaactgac agcagaagat gattcccttt cttatgaaga aatcttccga
    24661 acaaaataat cgttgtccaa acagaaaaaa gcgattgaac aagtgaaagt tttgccttca
    24721 tttgttcaat cgctttttat ttttattctg ctgtattttc ggaatgcgca tttgcgtcag
    24781 aatgcaagaa atagatcaag gtttgcaatt cattcgtcaa atcaacattt tggatcaata
    24841 catctgacgg agcattcaat cgagcagcgg taaagttcaa gattcctttg attcctgctt
    24901 ccgttaactg attgactact tcttgtgctc tttcagcggg tatcgttaaa attgctactt
    24961 cgatttgttg gatcttgatt tgttctgaca tatcttccat tggataaacg gggataccgt
    25021 ctacgatgcg cccaacgatt tcctcattca catcaaaagc acaactgata cgtatgctat
    25081 tactttgatg gaatttgtat tttagtaaag cacttcccaa gttccctaca ccgatcagcg
    25141 caacatttgt caattgatcg tcgtttaatg tctttgcaaa aaagtgcata agatcttcta
    25201 catcataacc atacccacgt tttcctaatt cgccaaaata agaaaaatct cgacggatcg
    25261 ttgcactatc tacttgaacg gcttcactta attcagttga agacactttt ttctttccag
    25321 cattatttaa aatccttaaa tagcgataat acaaaggcag tcttttagct gtcgcctttg
    25381 gaataatatg atctttcacg aaatgacctc cacgtattgt ctaattacat ctttgatttc
    25441 tccatcatag cagtatttat aggaaaaaat caattccatt gctcagatat tgtgaaattt
    25501 tattctttat tcatttattt atcatttctg caaatctcgc agtataatgg aatttatggt
    25561 agactactaa ttgaaaaata gttttttatt tgaggagaat tcacaaagta gaaaagagga
    25621 ttccatgata ttattacaag ccaatcagat tgctcgccat tttggtgcag aagtcttatt
    25681 tgaaaacata catttagaaa tcagtacagg cgctcgtgtc gctttagttg gcagaaatgg
    25741 cgctggaaaa tcgactttat taaagatcat tgctggaatt gaagcacctg atgaaggaaa
    25801 gatcgctaaa aataaaacag cgactctcgg atacctcgct caggacacag gacttgattc
    25861 caatgaaaca gtttggaacg agatgctgaa agcttttgaa caagttagac aaatggaaga
    25921 gcggatgcgc gagctagaga ttttcattag cgaaggtacg ccagacacac ctgcttatag
    25981 ttcttattta aaagaatacg accggctgca acacgatttt tcagatcgaa acggctatgg
    26041 ttatgaaaat gaaattcgtt ccgtattgca tgggtttaaa ttcgattctt cattttatga
    26101 tcagaaaatc gatactttat caggcggaca gcgcacacgt ttagctttag ctcgcatgct
    26161 tttgcaacac cctgacattt tgattctaga tgaaccaacg aaccatttag atatcgagac
    26221 actttcctgg cttgagaatt atctgcaaag ctatactggt gcattattga ttgtttccca
    26281 tgaccgttat tttttagata aagtagtcaa tgaagtctat gaattaagca gaagaaaaat
    26341 gcgccattac aaaggtaact attcgaaata tctagatttg aaagcagaac agttagcaag
    26401 tgaatggaag gcttatgaaa agcagcaaac agaaatccat aaattagaag acttcgttgc
    26461 tcggaacctt gttcgcgcct ctaccacaaa acgagcgcaa agccgcagaa agacattaga
    26521 aaaaatggac cggctggatc gtcctcaagg ggatgaaaaa caagcaaact ttctattcag
    26581 tatagaaaag ccttctggaa atgtagtctt gcaaatcgaa gatgccgcta tcggatatga
    26641 taatgtcacc ttatctgaac caatcaatct agatatccgt cgtcaagaag cgattgctct
    26701 agtaggtccg aatggtatcg ggaaatcgac gttgctgaaa tcaatcattc accagatccc
    26761 ttttatccgt ggaaaagaaa cgctagggac aaatgtcacc atcggctatt acgatcaagg
    26821 tcaagctgat ctgcatacaa acaagacgat cctagcagaa ttgtgggatg agcatcctac
    26881 cactccagaa aaagagatcc gaagtgcttt aggtgctttc ttattttctg gcgaagatgt
    26941 agaaaaacca atccctctgc taagtggcgg tgaaaaagca cgggttgctt tagccaagtt
    27001 agcgatgaac aaagaaaatt ttttgatttt ggatgaacca acaaaccatt tagatattga
    27061 caacaaagaa gtattagaaa atgcattgat cgattatgac ggcacactgc tttttgtttc
    27121 ccatgaccga tatttcatca atcggatcgc gacaaaagtc gtcgaactct ctgaaaatgg
    27181 cagcaaagtc tatctaggag actatgatta ttatttagag aaaaagcagg aagaagaaga
    27241 aatcgccgaa ttgttggcac aagatgagcc agaatctgct cctgctccca cgaacaaatc
    27301 tgattattac caaagcaaag aacagcaaaa gcttatccgt agccttcaac gaaaaattac
    27361 tcaggttgaa gaagaaatgg cgcggatcga tgaatggatc gatcagttgg aacaagaaat
    27421 ggtcgcgcca gaaaacctgg aagatcacgt aaaactcaat gagctgaacc aagatttaga
    27481 agctgctaga caagatcaag aaacaaaatt gacggaatgg gaagaactaa gcttggaatt
    27541 agaagaatat gaaaattaac gaaaaaacca aaacagaaac cattcatgct gttttggttt
    27601 ttcttttttt attttatcaa acaaaaattc catatttacc tttttcacca ataagaagac
    27661 caattactag tcattctcac taaagcttct aaataatagt aatcacccca aatacaaggt
    27721 gtatttaccc cattattttt gggcttatta taaacagact gtgttaaaaa gccatcttct
    27781 tcttccgttg tagcatatct ttctttcaat tgtcgcataa ttttccaaac agcagactgg
    27841 tataagttct tttctgtttt gtctaataga cctgattcta ttaattctaa taatccacaa
    27901 accatgatca ccatactaga tgtatctttt tcttctctgc tagaatcatc aaagatcaaa
    27961 tcccaataac aaatggtatc ttctggcaac cgattcataa aataattggc taatattttg
    28021 cttaaatcaa aatatttttg ttctttcaaa taacgataac ttaatgcaaa accgtaaatc
    28081 ccccatgctt ggcctctagc ccaacaggaa ttatcactat aaccttgatg cgtacttcca
    28141 tatcttggtt ttccatcttt gacatccata taaaatgtat gataagttga accatcttct
    28201 cttactaaat aatctgctgc attttgaata tgagcctgag cagctttttt atatttttga
    28261 tttccagttt cttcactcgc ccaatacaat aatggaagat tcatgcaaca atccatgatc
    28321 attctacctt gttgtgaagg atcactagga tctccccaag cttgtataac tcctgccttc
    28381 tcccaaaaac gctgaagtaa tgtttctgca gcatcaattg aagcttcctt catttttcga
    28441 tttcccgtta ttttataatc tgctacggtt gataaagaat acaaaaaacc aagatcgtga
    28501 tgattagtga taatttgaca gtcaatcctg tggataaaat ctatatctgc tttttcagct
    28561 gtttgttgaa ataaaggatc acccgtcttc tcaaataaca accaattaat tcctgtccaa
    28621 aatccttctg tccaaccaat attgtctctt aatggataac gtccttttat aggacactcg
    28681 cttgggaatt gatactgact gttactgata cgtttgatta acctctccgt gtgtctgcga
    28741 caacgctcga tctcattatt ccattctttt tccattaatt ctttataaca ttcatttatc
    28801 catttttctg gatgtattat ctcttcaaac atcactttat caagcatttc cccaccagcc
    28861 tttagcataa ttttagtctt tttttattgt ttttattttg gatagttagc ccggttgtcc
    28921 catcaaaatc aattttggga cgattgttcc atacttctaa actataatcc cgatcttgat
    28981 gagcaaccac tcctgttccg agccaatggg ttccttttcc aaatgtaccc tctaatccag
    29041 gaataaaagt gttttcccat gtcatcaagt ttgtattggg caaacccata atagctgtgg
    29101 attttcgttt aatagtatcg tcacttaaac agataatacc tgaaaagcca gcttcactca
    29161 ccctatagtt ttccttgtct tcttctatga ttattccttc tttctcagga gaacattcaa
    29221 tagcaaaccc tgtctcatat agaacatatt ctgtatctaa ttctactttg tgtacgcgaa
    29281 tctgccagcc ttcaaaagga ataagccaag ttctgatttt tgcctgtaaa gtaggttccc
    29341 atgtagaggc aagtacctct ttcttacata caatgtctcg tacattattt cgaacgatga
    29401 actgtccata ggttttgatg ctctcattct taaaactggt cgtgatatat gtgtccgctc
    29461 gctgaatccc tagcattgaa tccattgcca tttctgcttt caattgattc gctcgcggta
    29521 tcgaaaaacc aaattgacta gaataagcaa attttaaata tttttcattg gtcaatgtat
    29581 ggaaatttgg ccctaattgt cccgcattca aaaaaaatgt atgtccatta tcatgttgta
    29641 tgagcaaatt ggcttctgaa agaagtttag ttccttcacg aataggcata ggcatttctt
    29701 tagccgtcca atactcatgg tcttcaggta gcgccaacaa taaaaaaatc ttattcagcc
    29761 ataatggaga taacgtacta ttatagggtt cagtcaatgt caattgaggg taggtataac
    29821 caatattcag gatacctttt tcatcaaata tttctttttg catccaccat cttatattcc
    29881 gattgactaa ccccttgatt tcacctaatt taaaaggcaa aagatcggcc cacaacatag
    29941 ctgaccaaaa gcttgaaact gcaaaacgat aaatcatgct tcgaccaaaa ggcacatttg
    30001 caccatcttc cgcaaaaaaa tgaatatagt ctctagaaaa atcaatagct cggttcttat
    30061 atatctggct aatgggcttt tctggttcta gtacgctata aatcaaacca taataatgaa
    30121 atgcaaaagg attataataa tcttggcgac ctcttgaact gtctcgatac cagccatttt
    30181 gaagataaca attttctata ttatcaaatg cttctttcag tgctgattct ttaactggat
    30241 aaccaaattt ttggataatt gttccgacaa ttatcttaaa aaattgccaa ttaccatctg
    30301 cacattcata ttcaaaaatt tgcgagaacc aatgaataat ctggcttttt tctcctgtat
    30361 ctaatttttt ccataataat tgttcatgat gatataacgc taataacatt gcgggcattt
    30421 ctaccatacg ctggtctctt tcatgtattt ctccccaata atcgggatgg gaaggatctg
    30481 ttccagcttt tattcctgat agaattaatt tcaaccatcg ttcatctaat tggtcaattg
    30541 cgggccccgc tccccataaa acccgagaga atccttccat tcctgcaatt ttatttccat
    30601 atcctacccc ttgatgtata taatcaattt gtgcgccact tgctcgataa aacggctgga
    30661 gtggttgatt tatcttttta aataagtcaa agtaatcctg tctggtcctt aatagtggta
    30721 ctggatgttt cattgtattt tctcctcttt ttactaccaa atagctaatt ccgactgttc
    30781 taatcgaagt aagccttcaa taaaaaaata atcagaatag atcagtggca cattataagt
    30841 cagttctcta tggtatgctt ctgctccgcc tccaatgatc ccatcttcat ccatattcca
    30901 atttgcatag tgcttttctg tcttttggag tatattgata gcagcttcat gatacatttt
    30961 tgatcgtaca gaggaaacat atcctgctaa ttccagaaga ccgcatgcag cgcatactcc
    31021 agcactggta tcaatattcg aatcttttac agcaaaatca atgagaggaa tataaccagt
    31081 tttttcgatt ttctgaataa aatttactgc tatcttttct gctatagcta gatagtacgg
    31141 attttttgtg taacgataac ttaacgcgaa tccatataaa gcccatgcat ttcctctgct
    31201 ccaacaagaa ttctcattgt atccttgccc gccaagtaac cgaatatatt tccctgattc
    31261 tgcatcaaat tctgcgatat gcccggttga tccatctttc ctcactagat acttagctgt
    31321 tgtatctgca tgctgccgag caattttaga gaacctcgga tcaccagttt ccctcgaagc
    31381 ccaatacagt aatggtaaat tcatcatact atcaataatc atccaaccag gtttatggtc
    31441 attccatgct actaaatagt tccctgaaat attatagcgt ccagcaagta ccgtagctgc
    31501 atgtaaccct agttgtcgac tatgtttgtt ttgagactgt tgataatgtg caatagccga
    31561 tggtaagaat aaaaaaccta catcatgatg aacgtctgaa aaatggtaca gggcagattc
    31621 taataattgt tcttgttgta ttgctgcctc tttgaacttt tctcgttttg tcttgtgaaa
    31681 gagctgccat aaaataccac tccaaaaacc attggtccac cagtcgattc ctctgtcctt
    31741 tattacgtct gtatattttc cgttgtaaga aatatatgga attttattcc ccattctgtc
    31801 taattccgca tctatctttt cagaagctac attcaaaatt tttcttgacc attccttcgt
    31861 ctgttcattc atggatatcc cgccattctt ttaattttta tgaatgtgga gttgctccta
    31921 gaacgaacta gatggttaac attcgttatt ttttttactt atttgcagct ccaatgtcat
    31981 tctccttaaa tttagactgc gcccacacat aaatcaagac cattgggatc actgaagttg
    32041 ctaaaattag cataattgcg ccataattaa ttggctcacc tgtactagaa accgtcatat
    32101 ttttgataat taagggaacc gtatatttat ctttatctga aagcatgact aatggaaata
    32161 gatattgatt ccaacttccc ataaagataa gtaaaccact agtaataatc gtaggtgata
    32221 aaatcggtac agcgattttc gtaaaaatca cccactcatt ggcaccatcg attctggcag
    32281 cctccatcag tgctgtagga aattgactgc tatattgtcg catcataaac actgaagttg
    32341 tactggcaat cgaagggatg atcacacctg cataagtatt cgataatcct aaacgactca
    32401 taatgtaaaa taaaggaatc aatgtggtaa aaaaaggcac catcctagat agcataactg
    32461 tattgaacaa cagtttattc cccataaaac gatactttgc taatgcataa cccgccattg
    32521 tagaactggc tgtagctaaa actgtactga ttacagcaat aacaatcgaa ttccaaataa
    32581 tcaagggata attatatctc gccatgatgg cttgccagtt ttttaatagc atatccccaa
    32641 acttgaaatt gattccgcca cttaaaattt cttgattact ttgcgttgaa gctacaatca
    32701 tatataaata tggcatcaaa aatattaagg caccaaacac aataatgacg tacgtaaaaa
    32761 ttctgctttg tatcttttgt ttagtcattt ttatcacctc cgaatttaaa ttgaatagcc
    32821 gataatataa cagatacaaa caccatcgtc caggcaatag cagaggcttt tccaaaatca
    32881 accatttcaa aacttgtttt atataaattg actcctacga ccaaagctga attttctggt
    32941 ccattggaag tcccaaagaa tatatttggg atattgaaat tacccaagcc attgatagtg
    33001 gctaaaactg tggccattaa aatgattggc tttagcatgg ggatcgttaa taaaaagaat
    33061 tgccgaaatt tcgatgctcc atctatttca gcactttcgt atatctcttt agaaatccct
    33121 tgaagaccag ctaaaaataa aatagtgtta tatcctacac taagccaaat catcgtgatg
    33181 atcattgata ttcgtatcca aaaaggattt gttaaccact gtacaccgtt aattcctagc
    33241 gcattcaaaa atccattgaa taacccagtc ggattgaaaa aagtcatgaa tacggaagct
    33301 acagccaccg ttgatgtaat ggttggaata taatagacag ttctaaaaaa tccttttcct
    33361 tttaactgtg ctgaattcaa taaaacagca aagtaaatag ctaatagcat cgttattgga
    33421 attgatcctg ccatgataat caaaacattc gtgtaagatt taataaacaa tggatctcgg
    33481 aagatagcta gataattgtc aaatccaaca aattcaaatg agtagccaat tttcataaaa
    33541 ctaaaagcaa acgagataat gatgggaatc aaaatcgtca caataaagaa aataacaaaa
    33601 ggtgtgacca atccataagc acttttttta tgagacgtat ctgttgcttt tttcttgctg
    33661 ttctttattg tcttgcttgg taacgaagat tgcatcatcg ttaagtcctc ctaatatttt
    33721 ttaatcacca gttaatttta cgccgtactt atgagcaaaa ttttctgctt cttttagata
    33781 tgaattttca aaatcacctg tttgccaata gttatatgca gcggtagtca aataagacgt
    33841 agcatctgaa taagtggctg gtaaaacagt atttgatgta ttttcattga ttttattcaa
    33901 tagttcatag tatttttgtc ctccttagta gtcaaacttt ttctcagctg cttctgtttg
    33961 ataggccaat gagttagatg tagccacgcc cagctccagt gcgttggcta agcattcttc
    34021 atctgtcagc ataaatgtaa gaaattgttg tgccacttgt ggatttttgg catctttagg
    34081 gacataaaaa cttgatcctc ctgatactgc accttttcct ggattttctt ttgaaaaagg
    34141 aatcagtgga gccatgcgcc atttgtcaga tgcatcagga aaattcaaag acatattcgt
    34201 agcataccag ccgccagcaa caaacattgc agattcttga aatgctttct cagcatcttg
    34261 tgcaccgtag aaatgtacaa tatctgagtc gatcatttct ttgattaact ttgcagcatg
    34321 tttcgcttct ttagtattca gacggatatt tccttcttca tcaagcaaag attgattctg
    34381 ctgggctagc atgttgataa acaattcttg gtccccagat tctggcaagg caattccaaa
    34441 actaccagtt ttttctttta atgcttttgc cacctggata tattcttccc aagaactgat
    34501 ttcttcaaaa tctacaccta tttcagcaaa tttttttggt tgataataac aaagtgctgg
    34561 ggtaaaatca ctagcccaag gaataatcgt ttcattactc gtttgttgac gcaaaatatt
    34621 caatttttga tcataaaact gatcgccata tttgtcataa aatccatatt catcagcact
    34681 ataaaaagca tcttgaaatt tttccagcac acctgctgct gaagtgtctg ccatggagat
    34741 aatatccggc atatcttcgt gtgttaccaa ttttggggtg atcatttgta ctgttgctgt
    34801 tcccgaagct acattcacaa tttttaagtc aatatttgct ccagttttgc ggttatagat
    34861 ttttgcagca ttttcaacat agtttgtcag tttgccgttt gcgacatata cttctaatgt
    34921 agtggctttt tttatcattg gatctgcgat gtttgctgtt acttcttcgt tagtttttcc
    34981 acatgcactt aagctgaata atagggtaat gtttagcaaa gctatcacca atttgtatct
    35041 ttttttcatg tctatccctt ctttcttttc aagttcttat ataaaacgct ttcacttatg
    35101 tatattaacg gaa
//
LOCUS       DAYYCY010000010        33696 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000010 DAYYCY010000000
VERSION     DAYYCY010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33696
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.010"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..827
                     /locus_tag="ACHAZB_000406"
     CDS             <1..827
                     /locus_tag="ACHAZB_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8613113.1"
                     /translation="LTKKSKEYANIKIIKGYVRSMFDIAEILNYIEFNRTTKIIQSIT
                     APKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLLTFHDYTLFMITLYLGDRK
                     SETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTVIQVPEVVMTLLSEWKSVQ
                     ADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRINSIKRRHPDLVHLSPHKL
                     RHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIVDKAVFEAFQRGLNKCD"
     gene            complement(915..1307)
                     /gene="rpsI"
                     /locus_tag="ACHAZB_000407"
     CDS             complement(915..1307)
                     /gene="rpsI"
                     /locus_tag="ACHAZB_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFM8613114.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(1323..1751)
                     /gene="rplM"
                     /locus_tag="ACHAZB_000408"
     CDS             complement(1323..1751)
                     /gene="rplM"
                     /locus_tag="ACHAZB_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFM8613115.1"
                     /translation="MAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPTFTPHL
                     DTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLIETSVK
                     GMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(2285..2752)
                     /locus_tag="ACHAZB_000409"
     CDS             complement(2285..2752)
                     /locus_tag="ACHAZB_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HFM8613116.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(2872..4410)
                     /locus_tag="ACHAZB_000410"
     CDS             complement(2872..4410)
                     /locus_tag="ACHAZB_000410"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFM8613117.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            4490..5176
                     /locus_tag="ACHAZB_000411"
     CDS             4490..5176
                     /locus_tag="ACHAZB_000411"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFM8613118.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(5377..5904)
                     /gene="msrA"
                     /locus_tag="ACHAZB_000412"
     CDS             complement(5377..5904)
                     /gene="msrA"
                     /locus_tag="ACHAZB_000412"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFM8613119.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(5988..6665)
                     /gene="pgmB"
                     /locus_tag="ACHAZB_000413"
     CDS             complement(5988..6665)
                     /gene="pgmB"
                     /locus_tag="ACHAZB_000413"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFM8613120.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(6662..7792)
                     /locus_tag="ACHAZB_000414"
     CDS             complement(6662..7792)
                     /locus_tag="ACHAZB_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFM8613121.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(7792..9975)
                     /locus_tag="ACHAZB_000415"
     CDS             complement(7792..9975)
                     /locus_tag="ACHAZB_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HFM8613122.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(9972..10997)
                     /locus_tag="ACHAZB_000416"
     CDS             complement(9972..10997)
                     /locus_tag="ACHAZB_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8613123.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(11042..11869)
                     /locus_tag="ACHAZB_000417"
     CDS             complement(11042..11869)
                     /locus_tag="ACHAZB_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFM8613124.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(11866..12933)
                     /locus_tag="ACHAZB_000418"
     CDS             complement(11866..12933)
                     /locus_tag="ACHAZB_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFM8613125.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKHGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLIGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(12959..13795)
                     /locus_tag="ACHAZB_000419"
     CDS             complement(12959..13795)
                     /locus_tag="ACHAZB_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8613126.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(13792..14661)
                     /locus_tag="ACHAZB_000420"
     CDS             complement(13792..14661)
                     /locus_tag="ACHAZB_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8613127.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(14731..16014)
                     /locus_tag="ACHAZB_000421"
     CDS             complement(14731..16014)
                     /locus_tag="ACHAZB_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8613128.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(16028..17713)
                     /locus_tag="ACHAZB_000422"
     CDS             complement(16028..17713)
                     /locus_tag="ACHAZB_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFM8613129.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            17988..19013
                     /locus_tag="ACHAZB_000423"
     CDS             17988..19013
                     /locus_tag="ACHAZB_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8613130.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(19608..23261)
                     /gene="rpoC"
                     /locus_tag="ACHAZB_000424"
     CDS             complement(19608..23261)
                     /gene="rpoC"
                     /locus_tag="ACHAZB_000424"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFM8613131.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHADITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(23340..26957)
                     /gene="rpoB"
                     /locus_tag="ACHAZB_000425"
     CDS             complement(23340..26957)
                     /gene="rpoB"
                     /locus_tag="ACHAZB_000425"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFM8613132.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            27685..28653
                     /locus_tag="ACHAZB_000426"
     CDS             27685..28653
                     /locus_tag="ACHAZB_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8613133.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            28671..29576
                     /locus_tag="ACHAZB_000427"
     CDS             28671..29576
                     /locus_tag="ACHAZB_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8613134.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            29573..30379
                     /locus_tag="ACHAZB_000428"
     CDS             29573..30379
                     /locus_tag="ACHAZB_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8613135.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            30536..31504
                     /locus_tag="ACHAZB_000429"
     CDS             30536..31504
                     /locus_tag="ACHAZB_000429"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HFM8613136.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(31821..32345)
                     /locus_tag="ACHAZB_000430"
     CDS             complement(31821..32345)
                     /locus_tag="ACHAZB_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HFM8613137.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            32632..>33696
                     /gene="scm"
                     /locus_tag="ACHAZB_000431"
     CDS             32632..>33696
                     /gene="scm"
                     /locus_tag="ACHAZB_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFM8613138.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSS"
BASE COUNT         9551 a         7196 c         6321 g        10628 t
ORIGIN      
        1 aattaaccaa aaaatcgaaa gaatatgcaa atataaaaat cataaaagga tatgttcgtt
       61 ccatgttcga tattgcagaa atactaaatt atatcgaatt taatcgaact actaaaatta
      121 ttcaaagtat cactgcacct aaaaaaaatg ctttagaaga aaaaagaata caagaaggta
      181 agcaagcact atcatcaaaa gaacttacta attggataga agcagtaaat gatgatttta
      241 ataatcattt acttactttt catgattaca cactttttat gattaccctg tatcttggtg
      301 atagaaaaag tgagacttac gctttgcaat ggaaatatat tgattttgaa aaacaaacgg
      361 tccgtcttaa acatacttta gataaatatc aaagaaaaaa gtttacaaaa ggtcgaaaag
      421 ataccgtcat tcaagtacca gaagttgtaa tgacattgct aagtgaatgg aaatcggtac
      481 aagctgatca attgttgaaa ttaaaaatta agcaaacgtt agatcagtat ctctttactt
      541 atactaaacc atctggagaa gtaaattgtc ctgttcatgc cgattaccta aactatcgaa
      601 tcaattcaat taaaagaagg catcctgact tagttcattt atctccacac aaactccgtc
      661 atacttatgc aactatagct agacaaggtg gagcagatat gaatcaaatt tcaaatgctt
      721 taacacactc agatatttct acaacaaaga tatatgtgaa cacaccagat atagttgata
      781 aggctgtttt tgaagctttt cagagaggat tgaataagtg tgattgatat gtgttcttaa
      841 atgtgttctt aaaaacaaaa gaagcacttt ccaaataatt gaaaagtgcc agaaacgttg
      901 atatataaag gatattaacg ttttgagaat tgtgaagctt tacgtgcttt cttaagacct
      961 ggttttttac gttcgaccat acgtgcgtca cgagtaagta agccagcacg tttaagagct
     1021 aaacggaagt caggatcaac ttctagcaat gcacgagcga taccatgacg gatagctcct
     1081 gattgtccag cgtatcctcc gccgtttacg ttaacaaata cgtcatatgc gccttttgtt
     1141 tcagtaacag cgaaaggttg attgattact tcacgcaagt cagcatgtgg aatgtattct
     1201 tcaacatctt ttttatttac agtaattttg ccagttcctg gtactagacg tacgcgagca
     1261 actgcgtttt tacgacggcc tgtgccgata tattgaactt gtgccaatga aattccctcc
     1321 tattagatta agtttgtgat gtctaaaact tctggttgtt gtgctgcatg tggatgttct
     1381 gctccaccgt atacgttcaa ttttgtaaat tgtttgcggc ctaaagtgtt tttaggaagc
     1441 atacctttta cagaagtttc gatcaaacga cgagaatttt tagcacgcaa ttcgccagca
     1501 gtgattgatt tcaatcctcc tgggtagttg ctgtgacggt aataaatttt gtcagtcgct
     1561 tttttacctg ttaatttcac tttgtctgca ttgatgacga tgacgaaatc gccagtatcc
     1621 aaatgaggtg tgaaagttgg tttattttta ccacgtagta cagatgcaac aactgttgaa
     1681 agacgtccca atggaacatc agttgcgtct actacatacc atttacgttc tacttcgcct
     1741 ggtttggcca tatatgttgt acgcacgtta ttttcctcca atttaagttc tgatgtttga
     1801 catacacttc gagtttccgg ggctctttgt ggggcaaaca ataccattta acatattacc
     1861 cgctaatcta gtcaatgtca acgtttttct gcttgattga aaaatttata tgaaacttta
     1921 cttcttttct ttcttttata taaaagggtc gtttcctgtc aaaatgaaaa acaaaaagag
     1981 gctgtgacaa aagtcttgtc acaacccctt attttgaata aacggtgttc tcctgccaac
     2041 tcctcggtca caagtcacaa tatccataaa acataagaag ctgtttttta aaattgtttc
     2101 ttgtgactcg gagctgaacg gcaggcttac aacctcttgg taaacggtgt tcaatcagct
     2161 gaccctcggt attagctcaa aaaacggcaa aatatgagga actattttga cgtttgtttc
     2221 tctaatactc aggtcaaaac gctgatttca caacctcttt ttcggtcaag ttcaacaaca
     2281 gtttttacgc gtcgatctct ggcgctttgc ccaaatgagc ttggatcatc cagatacgtt
     2341 tttctgtggc tgttttgaat tcgataaaga tatcttgtgt tggatcgtcg ccttcttctc
     2401 cagaaacttc gattcctttt tggtaaagat ctgctagata acgatagcct gcaacaagtg
     2461 tttccaaacg ttctggaata gtacgatccc agcggccaat ttcatcaggg atttttgtgt
     2521 tatctgcaaa ttcacgcaat gtagaataag gtgaaccgtc taatgtgatc aagcgttcgg
     2581 atacttcatc taactgagcg ttcaaatcat ccatgaactc atccatcatt gggtgcaatg
     2641 taaggaaacc tggtcctctc atgtaccaat gtgtttggtg aacaacaaca gacatttggc
     2701 ttagatctgc aactaattga tttaatactt ctttggtttg ttggaatttc atctttcttg
     2761 cctcctaaca ttttcttaca cttaaaataa tagtagactt tcaatgaaaa gtacaacaaa
     2821 atcatttacc tgagaacgat tttcagttcg cttttttatc cgtaaagcca atcatttttc
     2881 agaatcagct aattcttctc gcagttgagc aaattgctga taagtctgtg ctaaatgctt
     2941 ttgtgtcgaa acaaataatg gatagatttt ctgatatttt ttatgatcag tttcttgtac
     3001 tccaacttgc tctttcaatt ctatcaattc ctgtgcttca ctgatacttt ggatctttcc
     3061 gatgcttctc cagcctaaaa tcacagctcc taatgcggat gcttctgtga cagtagggaa
     3121 aacaagcggg taacccagta catcagccat catctgacga aaaatcggag attgagcaaa
     3181 cccgcctgta gcacggatct ctgtgagctg attttcctgg caaactcctt ttaaaatccg
     3241 ataaaggttg agacagatcc cttctagaat cgccttcatc atttcttttt ctgtatgttg
     3301 ccgctgtaaa ccgataaaac tgccaaacgc atctgcgtcc caaagcggtg ctcgctcacc
     3361 aagtaaatag ggatgaaaaa tcagaccatt ggcacccgct ggcacaaaag cgatagccga
     3421 aagcagtgca tcatatggat tttctccttt ttctttagca gattgaacga cttcctgtaa
     3481 aaatgtatgc cctgcccaat caaagatgcc agcaccattg ctagtcgctc ctcccactac
     3541 ccaatgtttc tgatccagaa cataacaaaa cgtctccatt tctggatgga actgaggctg
     3601 agtagtaata aaacgaagtg ctccgctcgt tcctacggtc aaagcggcga tcgtatcacc
     3661 aaaagcacct aaccctagat tagaaagcgg cccgtctgcc cctcccaaaa caaatgatat
     3721 atcatttggc aaacctagtt ctgctgctgt ttttgatggt agacctgtta cttgataggt
     3781 ggaaggaaag acttgcggca attgctctat tcgaatgttc aagtagtcaa gcacttcttc
     3841 tgcccattgg aattcatgga tatcaaaata tccagttcct gaagctgttc cgtagtcgct
     3901 gatcagttcg ccaaacaatc tataaaagat atagtctttg ataccgacaa aataagcagt
     3961 ttttttgaat aattcaggtt ggctgttttg cagccacttg attttgttca atggtgtcat
     4021 tggatgaatc ggcgtcccag tctgccgata aaattctttg ccaagcacgg tatatttcag
     4081 tttttctgct tcgtccgctg ctcggttatc tgcccaagtg atcattcgag ataacggtgt
     4141 tttctcttca tccaacaata tcaggccatg catcgcagag gagaaggaga caaattgaat
     4201 gattgtgtct gaaggcaact gtttaattac ttcttttata acggtgcaga caccttgaaa
     4261 gatcaattcc tgatcttgtt ctgccatccc ttgtgtttct tggatcaaag ggtagcgaaa
     4321 ataagcggat gctgcttctt gggcttcatc tgtgaaagca actgctttgg tttgtgtggt
     4381 tccgatatct aatccgattg aaacgttcat tttttcacct caaattttat gtatgtacaa
     4441 aaatttagtt taagcttgcg tatcttggtt ttgaaaaggg ggagatggcg tgttacttta
     4501 ttttatcatt ggttgctgta tcggatcatt tttatgttta accgcccagc ggattccgtt
     4561 aggtcactct attatttatc ctagatctca ttgtgtcaaa tgctgtcgtt cgctttcttg
     4621 gtatgaattg attccgattc tctcgatcat cgtccaacga tttcgctgcc gctattgcag
     4681 gtgtcggttg cccttctact atttgttggc agaagcctta tgtggtggtc tttttgcctg
     4741 gttctttact ttttcttccg caagaaattt ttcgacgttc atatggatgc tttcggcttt
     4801 gctattttcg ttgatggatt tattttactt catggtccat tctcacacgc tattctgttc
     4861 ttgggcaatt ttatggattt tttggttgca ggccggcgtc ttccaatggc aaagtgtgct
     4921 gttaacgttt atcgtaggcg gtgtgtgcct tcgctatggt caatcctatc tcggagctgg
     4981 cgatactctg ttgatgcttt cttggagtgg tggcttgtct ctagaagagt tgcttcagat
     5041 cttgtttctt gctagtctgt ttggaatatc cgttttttct gtttacttta tctgtcataa
     5101 aaaaaagttg gaaaagctgc ctttcattcc ttttttgagc agtgcgttgc tgattgtcct
     5161 tcatcttagg tattgaaaaa aggttgtgac aaacgacttg tcacaacctc tgatatatag
     5221 tgttctctta ccaaatcctt gtgacttgga gctaggcgac aggttcacaa cctctccact
     5281 aaatggtgtt caatcagctg accctcagta ttagctcaaa atgctggttt tacaacctct
     5341 gatatacggt gttccataag tagctttttc ggcttcttat ttcttgacgc cccaatgtcg
     5401 acgaaggaaa cctgcacgtc cagaaccttc tcgataaaga ttgtaatgga gcttgttttt
     5461 cttgtaataa tcctgatggt aatcttctgc aggataaaat tcgcttcttg gttcgatttt
     5521 tgtcacgatt ggcttatcga atcgcccgct gtcttgcaat gcttgttttg aacgctctgc
     5581 aatatttcgt tcttcttcat cattatagta gatcactgga cgataagaat caccgcgatc
     5641 agcgaactgt cctaatgcgt cagtcggatc ggtttgctgc caatagattt ccaccaactg
     5701 atcataagaa ataacttctg gatcatattc gatctctact gcttctgtat gccctgttgt
     5761 gcctgttgtt acttgttcat aagttggatt tggtacatgc ccgccagtat aaccggatgt
     5821 cacagaaatg atccctggct gctcttcaaa tggtttgacc atacaccaaa agcatccgcc
     5881 tgcgaaaact gctttttttg tcatataaaa tccccctttt ttctcatcat accttgattt
     5941 ccaaaaggag gataacgttt tgcttgtgac agaaaaaacg gaaactatta tatacgtgat
     6001 acggcttctg ataaatgcca aaacgaacgt acttgatcaa aaacggttaa atcaggaatc
     6061 acatattcag cagaaggaag atttatgttt tcacctattc ccaatacatg catgttacca
     6121 gccaaagctg cttgacaacc agcttctgaa tcttcaatga ccaagcatgc atttggcggt
     6181 acattcaatt gctgtgctcc tttgagaaaa acttctggat caggttttgc ttttgatact
     6241 tgcgtgccgt caataaggac atcaaaataa cttgtcaaat tcgttttttc taaaatgagg
     6301 cgtgcatttt tgcttgctga tcccaatccg atcttgatgt ttgttttttt caagtactca
     6361 agaacagcta ttgcaccagg caaaacggat gtttcatcca ttttttgaat cgcttgaaga
     6421 tatagattgt ttttttgctc agcaaaggct gatttctctt tttctgagaa tttctgttcc
     6481 atttttccaa atgacagcaa tcgctctagt gactcggttc tggatattcc tttcagttgc
     6541 tcgttttctt tctcagtgaa tggaatggac agctggttag ctaaaaccag ccacgcttcg
     6601 taatgataac gagctgtgtc tacaagaacg ccatctaaat cgaatagtgc tgctttgatt
     6661 ttcatcgatt acctccttga aaatgaacga cttgaagtaa agatgcgatg tttttagctg
     6721 tgcgatgaag attatcagtg gcttgctgta aagcttcttg taaaggcaca gctgcaggaa
     6781 ggatcggaaa aacggccgaa atgccaacat ctgctgcttt ttcgcttcct ttaccaacac
     6841 ttccgcaaat agctatcaca ggaacgccct ctggtactct tctagcaatc ccaactggcg
     6901 cttttccttt tagactttgt ccatctagac gtccttcgcc tacgattacg aggtctgctt
     6961 gctgtacccg tttatcgaac tccagtaaat ccaatacgta gtcgatgcca gagactattt
     7021 tccctgaagc aaatgctaac agtcctgcac ccattccacc accagcacta ctgcccgatg
     7081 cctcgattac ttgaggggca aatctcatat aaaaatcatg catggcttgg tcagccactg
     7141 ctaattcatt ttctttcaag cctttttgtg gtccgaaaac aaaagaagcg ccttcttttc
     7201 cgcataaaag attagtaaca tcagtaatga cagtgatttt ggctttaatc agctcaatag
     7261 gcacgttcgt tttatcaact gtagcaattt tacctagatt ttctcccagt gcttctactt
     7321 tttgattatc ttgatcgtaa aaagcataac ctagaccttc tgccatgccg atgccgccat
     7381 cgttggtact acttccgcca acgccgatga tgatgtgtcg aacaccttgt tgcaacaatt
     7441 gtcgtatcag ttctcctact cctcgcgtgg ataacctaag tggctgttgt tggtttagtg
     7501 ggacatcttt caatccgacg atttctgcca tttcaaaaat cgcagtttga ccgtctgaag
     7561 cgtactggat ttctttgttt ttgccaaatg cattagtaac gactgctgtt tgccgcttca
     7621 accttaaact gtctgctaat gatgaaaccg ttccttctcc tccatcgcct ataggtagca
     7681 gatcatattc agcctgaaca aaaacttctt gaaaacctga ttgaatggct tgtgctgcct
     7741 ctaaggctgt cagactttct ttgaaagaat ctggcgcaat cagaatacgc attattttcc
     7801 cctccgttcc aagatcagtt ctttttccag ctggtattgg tctccattga caaaaatagg
     7861 taatgtttct ttcgtctctt tttttattac tatttgtttt tttgtcaaaa caaatagcaa
     7921 cctgtgattt ttccaaataa aaggaaaaga aattttttcc catgctgaag gaagtcttgg
     7981 agaaatagaa agagttccat tcgttactgt aatacctgaa aaaccaaata taactgctag
     8041 ccatagtgct cctaatgaag cagcatgaat tccgtcatct gatgaatgcg gatctggtcc
     8101 taaatcaatc aggcacgctt cttggaataa atgatacgcc cattccattt cttggatacg
     8161 gcaagcctct atggcgtgaa tcgctttact caacgatgag tcgtggattg ttttgttttc
     8221 atagtaatgc aaattcttag aaacgattgg ttgagaaaaa tactcaggta ataagtataa
     8281 cagcatgaca agatctgctt gcttgctgac ttggagttca tttacttcgc tacgggcata
     8341 atcaagtaaa atagactggc tgccaggacg ttctttgtaa gtggtcaaat caataactgg
     8401 cttactgaga tacgtgtcat cttgcggaat cagctgttct ttgttaggca ctggcaaata
     8461 taacttatct aaaaaatttt gacattcttt caaaaaaagc ggatcgccag cttgatattt
     8521 aacagctaag gccacattat aatgcgccag atagttcgta tatgcattat tgtcgatata
     8581 ttctgtgtat tcatctggac caatgactct gtgtatctct aaatgatcat cgacttttac
     8641 agctctgctc agccaaaaag ctgctgtttc atgaatgagc gtttttccct ttgtttctaa
     8701 aaacgattga tcctctgttg cttggacata ctcttgaacg gcataagcaa ggtctgcact
     8761 gatatgatgt tctgcggcag ctgaagcaac ttcttgtctt gttccggttt tgatattcaa
     8821 tgcagcaaat ttgggtgtct cttcctgacc agtgtgtgcg ctttcccatg ggaaaagtgc
     8881 accttgatag tgataatctc ttgctttttg ccgtgcgcct tctaatcgat cataacggta
     8941 cagcagcaaa ttctttgcta cttcaggaaa ctgatataaa aagaaaggca ggataaaaat
     9001 ttccgtatcc cagaaaacat gccctttata accttcgcca gttagtccct tcgcccctac
     9061 actaacactt ggatcattgc ggggagtcat aatctgtaaa tggtaagcag cgaagtccat
     9121 cgctaactga tcgaaagaag aatcgccttg gatcgttatc cgatgctgat gccaaaaatc
     9181 tgtccaataa gacatcgact gctctaaaat ttctgtataa ctaacgcttt tctgggcttg
     9241 ggcaatactt ttcttaaacg ggtctgtttg ctgaagagag gtagagagca cgatgatctt
     9301 ctccagcgta aaggacgtat tttttctgat cttttctgaa aaggaagcgg tgatccggcg
     9361 attttttgct tggaatacac ctgctttatt gaatttagct gcaatcccaa tatcaatttt
     9421 actttctgtt gtctggtagg atgcactgat caaatcgtca ccaaatactc gcagttcttt
     9481 ttcaaccaac tgctgtgtac caaaattggt ttgctgtcca tcgatacccg ttttcacatc
     9541 tacttggcag tcttcggtca acggcttgat gaccactttc agggctgcct gatggagatt
     9601 attttgatca gcaacacgac aagtaatgac ttccactttt tttccttgag cagtttcaat
     9661 gagcgcatag cgtttaagct ctcccatttc taaatctagt gcccgctcgt atgcaagaac
     9721 tttcatcgtc tcaagagaaa aatgttcacc gtcgattttt atttcccatc tgatgacatc
     9781 tgggagattc actaactcgg ctgattctgc tcctaaaggt cggttatata caccagctgc
     9841 aaacattccc cttacttgat tccgatagtc ttcctcctgg cttcctctga taccaaaaaa
     9901 tccatttcca atggtcatct gactcgcata cttattgata tactctaaat caaactgttt
     9961 ttcttctact ttcataaggt aataacttct cctgtttctg ccgaacgata taaattttcc
    10021 accagattct ggatgacata tccttgccag ccatctgcaa tcatctgagt ctggtcaccg
    10081 agcgcccgat tcacaaaatg agccatactt agttcatgtg ggtcattaac tggcgtggtc
    10141 ttttgtaagg aaaccagctt tccatctaca tcttcgaata gttcgcctgg atacagatta
    10201 gcacctgcct gatcgccaca aaaagccaca ttcagtttgt cttctctgat attcaaagca
    10261 aacgaagtat tcagttggat gatcttgccg ttttctaatt ccagtgtgcc aaaaattgca
    10321 tcttctactg tgaatttgct tggatcccat ttcccgaatt tccctgaatt ttttttcgta
    10381 ccaatttttt ggaagctgta cgcccgaacg ctttttactt taggaaatcc agtaatatac
    10441 atcgctgtat caagcatgtg aatgccataa tcgatcaaag gcccgcctcc ttgaagttct
    10501 ttgtcgataa acgttcccca gcctggaaca ccagaccgac gaagcgcatt cgcttctacg
    10561 taataaatat ccccgagaac atcaagatga ttcttgatca ttgttgcttc gtcagaaaaa
    10621 cgatgctgga atccataagc caaaatctgc ccgtttttat cggctgtctc tgccatcttt
    10681 cttgcttgat cagctgtcat agctggtggt ttctcgcaca tcactgaagc attagcttcg
    10741 agggcagcca atacatgttc gaagtgaaag cgattgggtg tacagacact cacgatatcg
    10801 ggagaaatct cattcagcat ttgtgtaact gatgtatacg catgagaaaa tcctgtacgt
    10861 cggcaaaagt cttctgctcg ctggctgact ggatcaacca cagcggccaa ttctaagaca
    10921 tctgatcttt tttgataata agcagcatgt actttttctg ctacttgtcc agcgcctatg
    10981 attgctactt tcttcattta tttctctctc ctttgcttat ttacttattg ttattctttc
    11041 tctattcttt ctctaaatca tttaaatgac gcgctgtttc cagacagttt ttcaagtatt
    11101 gcaatgattc ctgataaatt ttcacttcat tttctcctcg caaacgacat tcatatacca
    11161 tataaccttc atactcattt tgaagtagcg tttgaaaatg gcgagtaaaa tcaattgcgc
    11221 cagatcctgg ttggtagcga tgattatctg ctaaatggat atgacccaca tactcttttt
    11281 gcgtagtcaa tgcttttgaa atatcatctt cttcaatatt catatgataa aaatcagcga
    11341 tgatctgcac atgtttcaat tgattagagt caatgtaatg acgcgcatcc gctaacaaat
    11401 tgatcatatg gtcttggtat cggttcaaag gctccaaata aataaccgta cctgtttctt
    11461 cggctacttt atctaaataa cgaagtgatt cgctgactgc tttatagtct ccttctttgc
    11521 ttcggggtga aaccatcggc ggtaatcgat aagtgaacat tccccaagct gctgggataa
    11581 cgattccttt gcctccaacc tcgcttagtg cacgcaaaat ctcagcaatt tgacgtacac
    11641 cattaagacg ccgttcttcg ataaagtcac cgatccaccc atcgtagccg ttacaagctg
    11701 tcactactgg gatccctgtt tctttgattg cctgtttgac tgtgtcgata ttttctatta
    11761 acaacttacc gtcgatttcg tattcttcaa atccacagtc tttgataaat ctaaattttt
    11821 ctaaaatgtc tcttgggaaa aatgcttgat cttgcgtacc gaatttcatt gttctcctcc
    11881 tcggacttga acgcccattt tgatactttc ttctgggtgc tggtctacat aacgcatgta
    11941 agcttctgcg ctttctgcaa atggcacaac tggatcaatg atttcttcac aatccaagta
    12001 accgttcatc aataattccc aacatgtgtc ctcgattcgt ttacggttcc atcttgggta
    12061 atctgggttt ggttcactag atgctcgagc aaagacgatt tttgcattat tgaaatgtgc
    12121 ttcgcgacca agattaaacc cagctggaaa aggtttagca aaggcaacat aagaaatgat
    12181 cccgccatag ccgatcccta ttaaagaagc ttgtaatgcg gcagcactgc cactcgtttc
    12241 aataactgaa tctactccac gtttatctgt caattccttt agacgaaaac cgacatctgc
    12301 tttcatagga tctagcgtgt aatcggcacc gtgtttttct gctaattctc tacgctgcgc
    12361 tatgggatcg atcccaacga tggtagtagc acctgctttt ttagctaatt gaatcgcaat
    12421 ttgcccgata gcgcctaaac cgaagattgc tacagcatca cctgctcgaa catgtgcttc
    12481 ccggatacca cctaaagcga attgagcagg atcgtaacat actgcatttt tccattgatc
    12541 caaccgatca agtttacgta gtttataatt atcaacaccg tttgtaatca gcgtttcacg
    12601 aatgggtcca taagtgcaga ctaactctcc tttttgatac tctgtcacca aactgcccgt
    12661 ttcttcgatc gtaccgacaa tcatatttcc taattgaaat tcgccaaata ccacaccgcg
    12721 ttggcttcct tcttctcttg gcaagaatag gcgccattct tctgaaaatt cttcatcgat
    12781 aaatggactt aatcctctaa agtcgacaat ttctgttcct ggttttggag tagcgaaaat
    12841 tactcggata cgtacttcgt tcttttcgat ctcacggtct tcgtaatctt tgaatccggc
    12901 tattcttggt tctaatgcaa ctaatttttt catcttttct ttcctctttc ttttcttttt
    12961 atccttttac gccgccttcg actaaatcac ctttaataaa tcgttcagat aacgcataca
    13021 taatgatgac tggtaaagcg gtcaccaatg aagcggccat cattcgtccc caaatgtagt
    13081 ctggtgtact gaataatgtg ttcaaaccga ttggtaaagt aaatttttcg gaggtcgaca
    13141 ggaaaatcga agcaaataaa taatcattcc acgaaaccat aaagcaataa acgaagacag
    13201 aaatgattcc agaaatagcc aatggtataa tgatccgaat aatgatctgg atccggctaa
    13261 gaccatcgat cattgctgct tcttcaatat cagtcggaat cgtgtcaaaa taacttttta
    13321 gcatgaatat cgcagtagga agtgtttgaa cgaccatcgt gataatcagt gctgtattgg
    13381 tatcatacaa acctagaccg gaaatgattt taaataatgg aacaacgatc aaaataccgg
    13441 aaaacatata aacggtgtaa aagctagcat tgattttcga gcgagctttg aagcgtagtt
    13501 ttgacaaagc gtatgccccg ctgatcccaa tcactactgc agttacggag gctatcaagg
    13561 agatcactaa actatttttg aaatacatga tgaatggaaa aatctcagca ttgaaaatat
    13621 cctgataatg ttcaaaagtc cattctttcg gaaaaatagt ttgtgtcgtt gcaattgctt
    13681 cttgtgaatc cttgaatgat gtcatcagca tgatcaaaaa tggaaacaaa ttgacagcta
    13741 atagcaacaa aatcgtcgcg taaaataaga ttttcctgct ctttttattt ttcatttcaa
    13801 gtccaccttc tttcgtgaga gcaagacgac cgctgcaata atgatgaaca atagaatcga
    13861 tatagctgct gcttttccaa gatcattgaa cgcaaaggct gtctgataca aataaacgcc
    13921 taagatattg actttattcg tcagtagata gacatctgta aacatataga acatccagat
    13981 agatcgtaaa acaatcacag ttgctagtac tggtttgatt gccggcagtg tcacgatctt
    14041 gaacttctgc caagcatttg caccgtccat ttcagctgct tcataaagcg ttttgtcgac
    14101 tgtttgcaaa atagccaaaa atgaaataaa cgcatacgga tagtatctcc agatagcaaa
    14161 caaaacgacc ataaagaagc tagcaactgg ctggtcgaac cacagcggtg cttcgttgaa
    14221 aagctgcaac ctatctacta acagatcatt gacgattcca taactattat tgaacatata
    14281 tttccaagca aaaatcaaag aaatagatgg cgtaacataa cttaataaaa tcagtgaacg
    14341 caccgttttc ctaaatctga acttgcggtt aagcaaaata gctacggcta atcctaaagc
    14401 tgtactgcct gctacaacta agaatgtgta agcaactgtt aaacctaaag aacgataaaa
    14461 ttcactatca cttaaaattt caagataatt gcttaatccg ataaaaacgg cgtctgagtt
    14521 aggattcagc ggcatatcga aaaaactgat ctgaatattg gaaagcattg gaatcagcac
    14581 taaaacagct aataaaagca tgcttggtgc taaaagtccc atggctaatt ttaagtcaga
    14641 atgtttcctt ggacgattca ttgaaacctc cccctgataa tctgccaata gctatctact
    14701 tttttcgaac accattggca gaacttcttg ttatttcgta tttttttgtg cgtcagctaa
    14761 agcttgatcg acatcttcgt ttccgacagt tacttggttc actgcttttc cgatcacacc
    14821 tgagcttgtg atatctccca ttttcgtgaa attcttttcc ccgactagtc caaagacttg
    14881 tacttcatca aaagcactag caatctcaga tggcagctcg ccaaaagact tgatgacttc
    14941 attttctttg taaccatcca gttcgactac ttgtttattc actggttgag caccaccagg
    15001 agacatcaag acccactttt ccatgttttt cggttgagat aagaaagctg tgaatgcttc
    15061 agcagcctct ttttgtttct cgtctagacc agaagaaatc gtcaatccag atactgtgcc
    15121 atagacagct tgatcttttt gtgttgggat agcaaaaccg atattttctg agttccccgc
    15181 ttcataaaca gaaggcaaaa tgtaagttga atagattgcc attggtgctg ttccattcat
    15241 gaaagcatct ttgatttctg tcacatcatt agagcctggc atcgtgtatt gggataagtt
    15301 ttgataaaac gacaatgctt ctctcatctt ttccgtatcg atcgttacct cgccttcatc
    15361 atccaaaaca tttgctccat ttgacaaagc aaattgagag aacgcttgct cactcattgt
    15421 tccttcagca gtaggcatag caattccata ttgtttattc tctttatctg tgaaatgttg
    15481 agcaattttt aaaatatcat cccaattttc tggttctgaa aaaccagctt ctgataattt
    15541 ttctttgtca taccaaatac cttgaaccca gccgctaatt ggtgcagcaa tatagctttt
    15601 tccatcttct gaacgaacaa ggtttttggc tccgtcataa taattatctt ctccagcttc
    15661 ctcgataacg ttttggactg ccgtctgatc aatcagttca tctttatcca tcactttagc
    15721 aaaatcctga ctgacttcca ttacttctgg taatttaccg gaacgagcta acgtaacgat
    15781 ttttgtattg aacgctgatt cttctaccgg cacttgcttg attttgatgt ctggattttc
    15841 tttttcaaaa tcagcaacca gttcattgat cacacttaat cgttcctgtt cgacagaaga
    15901 gtgcatgaat tgaatcgttg tctgttgtga ttcgttagag ctttgacttt caccacaagc
    15961 agtaagaaga aatccagtag ctagcagaca ggtagtaaca ataatttttt tcattttttt
    16021 ccacctctta ttttattatt ttcagccaca tatattcata cggctggaat atcaactttc
    16081 gttcttgact gcaatggata atttgtccag aaaacaggtc taggtaatct ccttttggta
    16141 aaagataacc tgctgattga ttggaaaaat tatggataac taagactttc tcttccttcg
    16201 cttcgcgaat aaaactgata aaagaatctg ctgagtccag taagtaaatc aaactattcg
    16261 gatggaaggc tttttctttt tttcgaatag atagcatctg ggtcaattca ccatatactg
    16321 ctgttcttaa tggatctgtc tgcaactctt tctcaatatc tgctaacaaa aatttttgcc
    16381 gattgatttt tcgattctct cctgtttttt ctacgccttt gtaatcattt cgacttccca
    16441 gcacactttg aatataaatt gccggcacgc ctggtaacgt taccaaaaat gaatgagcag
    16501 ctaaaaagcg tttgcttctc cattccgtcg tatctgttgg agaacttaac gcatctagat
    16561 acgtagcgtt tatctcatat ggactggtcg ttccatctgg gtttttcttg tagtttactc
    16621 gggcaccctc tttcttcagc tgttccacta attcaaggat cgttttttcc ggcagaatac
    16681 ctcgtaccgg attcaatccg ataccgtcat gggaggctaa aaaattaaaa aaagttgttt
    16741 ttttcgatgc gaaatcgatg gtttttaacc attctttcaa cttacttgta ttacccgaac
    16801 gaatcgcttg aagggtcaaa ggtggtagcg aaaactgata gaccatctgt gcttcattct
    16861 cgccattccc aaaataactg atattatctt tgtgtggcac atttgtctca gtgatcaata
    16921 ccgttcctgg agctgcttca tcagcgattg cacggaataa ttggatgatt ttatgcgttt
    16981 ctggcaggtg tatacaagaa gttcctagtt ctttccataa aaatcccaca gcgtccaagc
    17041 gaataaatga agcgcctttt tctaagtaaa acaaaagaat atcgatcatt ttcaataaaa
    17101 cttctggatt tttaaaattc aagtctactt gatcttcgct aaaagtcgtc catacgtgtt
    17161 tttctttccc gtttttgtca atgaacgttg taagtaacgg gctggtcctt ggtctagtca
    17221 cgctgcttgt atctgtatcc agtggcaaat caataaaaaa atctttgtac gtatcattca
    17281 agtttaaata ttcctgaaac caagtacttt cggcagacat atgattgcag acaaagtcga
    17341 acattaaccg tgtttcgctt ctcatttctt ctatatcttc ccatgtgccc aaacggtcgt
    17401 tgattgcctg ataatttaca acggagaaac catcatcaga tgtatatggg aaaaatggca
    17461 agaaatgtac aactctaaat gtatctgcca aatacttttg ataaaatttc ttaaatgtac
    17521 gcaaagttgg ttgctgctct tcaaaaaatt gatccccata agtaatcaag aaaatatctt
    17581 cttcgtccca atgcagtttg cctgcctggc ctttcttctg ctgataaaca ctgacttttt
    17641 gttcaatcat agaggcggtt ttctcagaca aatctgagtc gcgataaatc gcttgtaaaa
    17701 gttttgtata catcggattc ccctttcttt tttgggagcg ttcccaacga tttgatcata
    17761 ttatggaaaa cgcttactgt caatagcatt tttttaaata attttgatat acacaaaaat
    17821 acaaataaga aagcttatgt atcaataatt aatagcttct taaaaaaata tggtctctca
    17881 tgatcatgca agctattcct aaattttcaa aatcatagac aaaaaagaat gtctattgta
    17941 ctatggaatt attctaaaac gctcccaaaa aggaggtgca tataaaattg aaacctacta
    18001 tttatgacat tgcgaaaaaa gctaatgtct caaaatcaac tgtctccaga gtattaaata
    18061 accagccaaa tatctctgat aaagcaaaag caagtgtact tcaagcaatc aaagaattga
    18121 attatgaacc gagcaaaatt gcaagaggac tgtcaggaaa tggttttgac gcgattctag
    18181 cattatctca tcgtacttct catacgacct atggcaatcc ttttttttca gatgttattc
    18241 aatccatttc tgccgtttct gaacaaaata acttcgactt gattctacaa acggcaaaaa
    18301 caccagaaga agagctagaa aaatgcgcga aaaagatcca agaaaaaatg atccgaggaa
    18361 ttattatttt aagctctacg atggatgaaa cacttctttt tgaattggat caatataata
    18421 ttcctattgt ggtaatcgga aaagtcggaa acgactacac gaacatctac tcagtggaca
    18481 cggataattt ccaggatagt tacgatttgg tggaaacact gatcaagaat ggacaccgtc
    18541 atatcggctg tctccatgct ccgacagaag tttatgtatc caatgatcgt ctcaatggtt
    18601 accgtcaatg cttatttgat caccacctag atatccgtaa tgaatggatt atagacggcg
    18661 gctttcgctt agaagatagc atggcagcaa tgaatgattt aatgaagcta cgagaaagac
    18721 caactgcgat tttttctacc gatgcattaa aatcattgag tatttacaaa gtggcagaac
    18781 aatctggaaa gcagatacct gatgatcttt cgatcgcagg attcagtgat cccttattgt
    18841 cctccctatt ttcgccacag ttgactcgga tagacattcc gacaaatcag ttaggaatca
    18901 aagccacaga acttttattc caactaatcc accaccaata ccctgaagaa aaaagaatca
    18961 ttctttcaac cgagattact ctcacagatt cgatccaaca acaagaaatg tagaacagaa
    19021 ttataaaaga acagaaacac tgaggataag tcagttactc tgttcttttt tcaatcactc
    19081 atccctcgtt accccctccc ggtattttag cgaatttttc ttattttcca tctgttttag
    19141 atagctgtgg ataactataa tgtttgtgtt aaactatttt ttccacaggg aagaaaaaac
    19201 tatttttaac cacgttgtgt ataagttttt atagttcgtt tttttaaaca catttaccct
    19261 tatttcaatc catgttattt ggggatactt atctctaaaa cataaataga ataacagaca
    19321 tgtctactgg ttttgtttta ttcgaggaat tattcaataa tagctagcaa aatattaaaa
    19381 ataaccgctc tatcttccca tcaatttatt ttgcattcaa aaaatgatct atctttactt
    19441 ttgttgattg acttctcttg gaagatgatc gttccgatac cttaattgga tacgaaccga
    19501 tgttgttaag atcctttccc acttttcttc tagttagaca aattatttta gacaaaccaa
    19561 aaaagaggtt gtgacaataa tttgtcacaa cctctttttt aaaatatcta ttttgtttga
    19621 ttgttcattg cgtcttctgc agccatttgc gcttcaatat ctgaaatact gtagacattt
    19681 tcgcttgcta caccgacttc ttttggttcc atgtcacgat aacgttgcat accagtacca
    19741 gctgggatga tcttacctat aataacattt tctttcaaac caagaagagg atctttctta
    19801 ccgcggatcg cagcatccgt caagacacga gttgtttctt ggaatgatgc agcagataag
    19861 aaactatttg tttctaatga tgcttttgtg atacctagta agacaggtcg agaagttgca
    19921 ggaacgcctc ctgagactag cgtattgtag ttttgatctt taaagtctgc gatgtctaat
    19981 aatgtacctg gtaagatatc tgtatcacct ggatccatta cacgaacttt acgcagcatt
    20041 tgacgaacca ttacttcgat atgcttgtcg ccgatttcta ccccttgcat acggtaaaca
    20101 cgttgtactt cacgaagcaa gtagttttca acagataata catcgcgaac ttgcaacaat
    20161 tgttttggat cgattgatcc ttctgtcaat ggtgcaccac ggtggatcat atcgccttct
    20221 gctactttca tacgtgctgt ataaggaaca gtatacgtac gtgtatctgt ttttcctttg
    20281 attgttactt ctttggtacg tgttgcagga tcttctgaga tatcgatgac atcccctgtt
    20341 acttctgtga tcactgcttg ccctttcgga ttacgtgctt caaagatttc ttggacacga
    20401 ggaagacctt gagtgatatc gtctccggca accccacctg tatggaatgt acgcatcgtc
    20461 aactgagtac caggttcacc gattgattga gcggcgatcg taccaactgc ttctccgact
    20521 tcaacgtcag agccagttgc aaggttgcgt ccgtaacaat gtttgcagac accatgtttt
    20581 gtattacatg tgaagacaga acggattgtc actgtttcga cacctgcatc aacgatttgt
    20641 tttgcgatgt cttcggaaat caattggttt cctgcaatga tcaattcatt tgtttcagga
    20701 tgacgtactt ctttacgggt gtaacgtccc actaaacgat cttctaatga ttcgatgatt
    20761 tcatttccct cgcggatggc ttggatttca agacctcggt cagtaccgca atcttcttca
    20821 cggatgatta catcttgggc cacgtcaacc agacgacgag tcaagtaacc tgaatcggca
    20881 gtcttcaagg ctgtatcggt catcccttta cgagccccgt gagttgagat aaacatttcc
    20941 aagacggata gtccttcacg gaagttcgaa atgattggca attccatgat tcgaccatta
    21001 ggagcggcca tcaaaccacg cattccggca agctgtgtaa agttggaaat gttaccacga
    21061 gctccagaat cagacatcat gaagattgga tttttcgctt ccaaaccttc catcagtttt
    21121 tgttggatgc tgtcttttgc agcattccaa acagcaatga cacgttcata acgttcgtca
    21181 tctgtgatca gaccacgacg gaattgtttt gtaatagttt caacttgttt atgggcattt
    21241 tcaatgattt cttgtttttc attcagtaca ctgatgtcag cgatccctac tgtgatatcg
    21301 gcatgtgtcg aatgtttgta ccctaagtct ttcatacggt caagcatctt agaagtttct
    21361 gttactttga atcgtttgaa gacttctgcg atgatgtttc ctaagttttt cttcttgaac
    21421 ggtaatacta attcttgctc tttgatatgt gcaggaatat cagtaccagc ttcaacaaag
    21481 tatttgtccg gtgtttggac ggtcaagttg aagtctgttg gttcattcag ataagggaat
    21541 tcaggaggca tgatctcgtt gaagatgatc ttaccaactg ttgtgatcat cgtacgttct
    21601 ttttgccatt cagtaaatgg tttttcacct aatgagttag ggttcacacc gatacgtgaa
    21661 tgtaaatgaa catatccgtt gcgccatgcg ataacggctt cgttttgatc tctgaagacc
    21721 attccttcgc cttcgcgtcc ttcttcttcc attgtcaagt agtagttacc tagaaccata
    21781 tcttgagaag gagtaacaac tggtttacca tctttagggt tcaagatgtt ttgagcagct
    21841 aacatcaaca tacgtgcttc tgcttgtgct tcttcattca atggaacgtg gacagccatt
    21901 tggtctccat cgaaatcggc attataagct tcacatacaa gtggatgcaa acggattgcg
    21961 cgtccttgta ctaaaactgg ttcgaatgct tggataccaa gtcggtgaag cgtaggtgcc
    22021 cggttcagca atactgggtg ttccttgatt acgtcttcta aaacatccca tacttcgtct
    22081 tcttgacgtt cgattttacg tttcgcattt ttgatgtttg aagctagttc gcgttgaaca
    22141 agctcacgca tcacgaatgg tttgaataat tcaatggcca tttcttttgg aagtccacat
    22201 tggtacatct tcaagaaagg tccaacaacg ataacggaac gacctgagta gtcgacacgt
    22261 ttacctagca agttttgacg gaaacgacct tgtttccctt tcaacatatg agaaagagat
    22321 ttcaatgggc ggttacctgg tccagtaact ggacgtccgc gacgaccgtt atcgatcaat
    22381 gcatctactg cttcttgcag catacgtttt tcattttgaa cgatgattcc aggtgcattc
    22441 aagtctaaca aacgtttcaa acggttatta cggttgatta cacgacggta aagatcattc
    22501 aagtcacttg ttgcaaaacg tccaccttct aattgaacca ttgggcgtaa atctggtgga
    22561 ataacaggga taacgtccat aaccatccag cttggttgat ttcctgaagc gcggaatgct
    22621 tctaaaatat ccaaacggcg gattgcacgt gtacgttttt gtccttgtgc agatttcaat
    22681 tcttctttca attcggctgc ttctgcatct agatcaacat tatcaagtaa ttgtttaatc
    22741 gcttctgcac ccatagcagc ttggaattct tgtccatatt gttcacgttt ttcgcgatat
    22801 tcgcgttctg tcaacaattg ttttttctct aaattcgtat ttcctggttc gatgactaca
    22861 tatgaagcaa aataaatgat ttcttccaaa gcgcgtgggc tcatgtctaa tacaagaccc
    22921 atacgtgaag gaatcccttt aaagtaccag atatgtgaaa caggagctgc taattcaata
    22981 tgtcccatgc gttcacggcg aactttcgaa cgagtaactt ccactccgca tcgatcacaa
    23041 acgattcctt tataacggat acgtttgtat tttccgcagg cacattccca gtcttttgta
    23101 ggaccaaaaa tccgctcaca gaacaaccct tcacgttcag gttttaaggt acgatagtta
    23161 atcgtctcag gtttttttac ttcaccgtaa gaccagcttc tgattttttc gggagaagcc
    23221 aaacctattt gcatactttc gaatttattt acatcgatca aaaggggttc cctccatttc
    23281 atttaatggc aaaccgcaag acggctgatc ttcgaccgcc ttgcagctca tagaccggat
    23341 tagtctaaat tatcttctgc ggtttcaaag ttttggatga catcgtcttt ttcatcttcc
    23401 acttgttcag cagctttctt ttctaattct ttggcagttt gctgttcggc aaattttgtc
    23461 aaagcatcaa cagtgatcaa atcgtcgtct tcatcgtcca tgtcgcgaag ttcgatctct
    23521 gtttcttcga tatccaagac acgcatgtcc agacctagtg attgcaattc tttcaccaat
    23581 acgcggaatg attccggtac acctggtttt ggaattggtt cacccttgac gattgcttcg
    23641 tatgttttca cacgtccgac tacgtcatct gatttgtatg tcaagatttc ttgtaaagta
    23701 taagccgcac cgtaagcttc cagtgcccat acttccattt caccaaaacg ttgtccacca
    23761 aattgagctt tacctcccaa tggttgttgt gtaaccaatg agtaaggtcc gattgaacgt
    23821 gcatgcaatt tgtcatcgac catgtgagca agtttgatca tgtacatcac accgacagac
    23881 acacgtccat caaatggttc acctgtacgt ccgtcgtaca gaacagtttt cgcatcgctg
    23941 gccataccgg cttcacgaac tgtttcccat acgtcttcat cacttgcacc atcaaatact
    24001 ggcgttgcta cgtggatacc taactgacgt gcagccatac ccaagtgtaa ttccaatact
    24061 tgcccgatgt tcatacgtga aggcacccct aaaggattca gcatgatatc gattggtgtt
    24121 ccatcaggta ggaatggcat atcttcttcc ggcataatac gggatacaac ccctttattt
    24181 ccgtgacgac cggccatttt atccccttca tggattttac gtttttgaac gatataaaca
    24241 cgaactaaca tgtttacgcc tggtgacaat tcatctccag cttcacgagt aaagattttc
    24301 acatcgtgaa cgataccgcc gccgccatgt ggtacacgta atgatgtatc gcggacttca
    24361 cgagcttttt cgccaaagat cgcatgcaat agacgttctt ctgcagataa ttctgttacc
    24421 ccttttggcg tgactttccc tacaagcaaa tcgccatctt ggacttctgc accaatacgg
    24481 atgataccca tttcgtctaa atcttttaat gcgtcttccc cgacattagg gatttcgcgt
    24541 gtgatttctt caggtcctaa ttttgtatcg cgggcttctg attcatattc ttcaatatgg
    24601 atagaagtgt atacgtcatc ttttaccaaa cgacggctca tgatgatcgc atcttcatag
    24661 ttgtaccctt cccaagtcat gaaaccaact aaaacgtttt ggcctagagc catttcgcct
    24721 tgttccatag atggtccgtc tgccagagta tccccttttt cgactttctc acccaaatga
    24781 acgattggac gttggttgta gcttgttccg gagtttgaac gacggaattt tgttacatcg
    24841 tatttatcta atgcaccgtt gtcgcggcgt acgcggattt cagaagcatc aacgtattca
    24901 acgacaccgt catgtttaca taacaaagca gctcctgagt catgcgctga tttgtattcc
    24961 ataccagtac caacccatgg tgcttgagga ttgatcaacg gcactgcttg acgctgcatg
    25021 ttggcaccca tcaaggcacg gttggagtca tcgttttcca agaaaggaat acatgctgtc
    25081 gcaacggcaa ctacttgttt aggtgatacg tccatgtagt ctactttatc aatagatact
    25141 tctaagtttt cactttgagc acgagccata acaacgtctt gagcaaatgt tccatcttca
    25201 ttcaatgggc tgtttgcttg ggctacgatg taatggtctt cgatatcagc tgtcaagtag
    25261 tcgatttggt cagtaacacg acctgtttca cggtctacac gacgataagg tgtttcgata
    25321 aatccgaatt tatttacttt cgcataactg gacaaactat tgatcaaccc gatgtttggg
    25381 ccttcaggag tttcgatcgg acacatacgg ccatagtggg agtagtgaac gtcacgaact
    25441 tcataaccgg cgcggtcacg agtcaatccg ccaggtccta aggctgaaag acgacgtttg
    25501 tgtgtcaatt cgccaagagg gtttgtttga tccatgaact gtgacaactg agaagaacca
    25561 aagaattctt tgatacttgc cacaactgga cggatattga tcaattgttg aggtgtcaat
    25621 gtttcagtat cttggatcga cattctttca cgaaccacac gttccatacg tgctagcccg
    25681 atacggaatt ggttttgtaa aagttcaccg actgaacgga tacgacgatt tcctaagtgg
    25741 tcgatatcat cgacattgcc gataccttcc atcaagttca agaaatagct cattgaagca
    25801 atgatgtcgg ctggacgaac cgtttttaca gctgcttctg gatagccgtt tccgatcacg
    25861 tttacttcac gttcagggtc tcttggtgaa aatactttga tgacttggac agtcattgga
    25921 tctgtgacca caccgtcttc tgatggatag taagtcactg aatttaggcc attatcaatg
    25981 aaaggagcta gtgtttccat gacttggtga gtcagaactg ttcctttttc aacgatgatc
    26041 tcgcctgttt ctggatcaac taatgtttca gctaatgtca gattcaatag acgtgttttc
    26101 aaatctaatt ttttgtttac tttgtaacga ccaacgtttg ctaagtcgta acgttttgga
    26161 tcgaagaaac gagcattcag caagttacgt gagctgtctg ctgtttttgg ttcgcctgga
    26221 cgcaaacgtt cgtaaacgtc tttcaaacct tcttctgttc ttgagtcgct tgcgttttta
    26281 tgcaagtctt tttcgactgt attgcgtaat gtttcgctgt cgccgaagat ttcaaagatc
    26341 gtatcatcgg aaccaaatcc taacgcacga actaatacgg ttaaaggaat cttacgtgta
    26401 cggtcgatac gtacataaga gatgtctttt gcatcagttt ccatttctag ccaagctcca
    26461 cggttaggga taacagtaga accgaatcct tcttttccgt ttttgtcgac ttttccatgg
    26521 aaatagacac ctggtgaacg gactaactga gaaacgatta ctcgctctgc cccgttgatg
    26581 atgaatgttc cttgttctgt cattaatggg aaatcaccga agaatacttc ttgtgctttg
    26641 atttcgccag tctcgcggtt tgtcaaacgc aatgttacat gtaatggtgc agagtagttt
    26701 gcatcatgcg cacgagcttc tgctactgta tactttggtt ctttcaattc ataatctaca
    26761 aattccaaag atagattgcc attgaagtca tcgattggca aaatgtcttc aaacatttct
    26821 ctaagtccct catctaagaa ccattggtaa gagtctgttt gaatttcgat caaatttggt
    26881 aattccaaca cttcactgat tcttgcgaaa cttctacgtt cgcgatgttt tccgtatttt
    26941 actacgtgtc cagccaagct cttcacccct ctaaaatgtc attcaaaaag actacccgat
    27001 caattgttac aataatattt caaatattat ttttaggtaa caacagacat tttttgcggc
    27061 ttttaggcaa aaaaaaacca aaaatagaac attctcttga aaccttactt cgtgagcttc
    27121 tacttttgtt tccttatatt aaagccgaaa cggacaatat ttcgtttctg ccttttcttc
    27181 atcagatagt ttttgcaact tcatatacta cacttatttt ctataaaagt caacacttcc
    27241 tggcgtttct tctgcttttt cgcaacattt tcttaatgtt ttcaatttta tgttttcaat
    27301 tatctgacaa ttcttaattg acagaccttt ttatctcggt tatactttag aaaattcatt
    27361 ttgtggagga acttctatgc gaaccaacat caaccaaatc gcaccaacac aactgaataa
    27421 cttttacttt agctacttta gataggtttg tattcatcat gatggataca aacgttaaac
    27481 gacagtttgt atctatgatg gctaaagaac tttgtattgc agcaaaaagc cacgtagaca
    27541 aaatgctaag tggctgagca gtgaaaatac aaagtaaaag tatcaagcat tctacttttt
    27601 caatttattt tccgactttt caatccattt ggacattttg tctgaatgga ttttttgttt
    27661 cccaaaatta aggaggaaaa agaaatgaaa aaaaatataa tcatcggatt aggcgctgcc
    27721 ctgctactag ctttaggagg ttgcgcacaa aaagacaatt ccgcagaggc atcagatgga
    27781 caaaaaacaa ttggcgtatt gcaacttgta caacataact cgttagatgc tgcctataaa
    27841 ggattcaaag aaggattagc agaagaaggg tataaagaag gcgataatct aacaatcgat
    27901 tatcaaaatg cccaaaacaa tcaagacaac ctgaaaagta tgagtgagaa actcgtcaaa
    27961 gaagatcctg atttgctact tggaatcgcc actccagcag cacaatcttt attaaatgaa
    28021 acaaccacta tcccgatgac tgtcacagcg gtcacagact tggaacaagc ccatttagtc
    28081 acatccaatg aaaaacctgg cggcaatgtg actggcacga cagatatggt gccaatcgaa
    28141 aaacaaatcg gcttactgtt aaatatcgtg ccagacgcca aaacaatcgg catcatgtat
    28201 aacaacggtg aagcaaactc taagatccaa gctgatttgg cagaaaaaga attgaaaaaa
    28261 gcgggcgtca aagtcaaagt actgacagca aattccacaa atgatgtcca acaagtgacg
    28321 aaaagtctag ctaaagatgt agatggaatc tatattccta ctgataacac gttcgcaagt
    28381 gcggcaagtg ttgtcggtga agtcgcaaaa gaaacaaaaa cacctgtcgt tgctggcgca
    28441 gttgatcaag tcaaagacgg cggtctagca acagtaggaa tcgactacga aagcttgggc
    28501 aaacaaacag ggatcatggc agcaaaaatc ttagacggca agaatccaag tgaacttcct
    28561 gtcgaaacag cgaatgattt ggaactgtat gtcaataaag acatggccaa agcattaggc
    28621 atcaatccag acagcatcaa agaagctgaa taaaacacag gaggattctc atgacaaatt
    28681 tgctctctct tttcttatca tctgcatcgc aaggggtctt atgggcccta cttgcgattg
    28741 gtgtatacgt cactttccgt atcttggaca ttgccgactt gactgctgaa ggaagctttc
    28801 ctttaggtgc agggatcgcg gcagtaagta ttacaaatgg ctatcatcca ttgacagctt
    28861 gtcttctagg ctttttaggg ggtgcggcag cagggcttgt ttccggactg cttcacacaa
    28921 aattgaaaat tcccgccctt cttgcaggga tcatcaccat gactggttta tattctgtca
    28981 catcccgtgt gatgggtgcg ccgaacatct ctttattggg tcagccaacg atctttcatt
    29041 gggtcgagtc attcggcata acaaaagaca acgctgtact gatcgtcggt ctgctttttg
    29101 ctttgatcgt tgtatgttta cttgttttgt tcttacgtac agaaacagga cttgctattc
    29161 gtgcaactgg cgataatctg gcaatgagcg aagcaaacgg gatcaacaca gataatatga
    29221 agatcatcgg ttatatgatt tctaatggat gcattgctct ttctggctcc ttacttgcgc
    29281 aaaacaacgg ctttgctgat ttaaattctg gtattggaac gatcgtaatt ggacttgctt
    29341 ctattatcat cgcagaagtt ttgttacgta accagccact atttctccgt ctcttgactg
    29401 tcgttttagg tgcggtcatt tatcgtttta ttttagcgtt agtcttcgaa ctgaatgttg
    29461 aaccaagtga ttcgaaactt gcttctgctt tcgtgctagt gatctgtctt tctttcccgc
    29521 agatccagca aaaattccat ttacctcaaa aaaagaaagg cggtagtgtc aaatgaatga
    29581 cgtcttaaca ttggaaaatc tccatcatac ctttgaaaaa ggaacgatca acgaaaatca
    29641 tgtcctgaaa ggcattaatc tttccttaga tcccggcgac ttcgtgacga tcatcggtgg
    29701 aaatggggct ggtaaatcta ctttgctgaa cagcatcgcc ggtacatttt ctgtcgatca
    29761 gggtcaaatt ttattgaatg gaaaaaatat cacgaaaaaa tctgttgtcg aacgttcgaa
    29821 atccatcagt cgcgtattcc aagatcctaa actagggaca gccgttcgtt taacggtaga
    29881 agaaaatctt gctcttgcta tgaaacgagg aaaaaaacgc ggcttcttcc gtggtgtcaa
    29941 accacaagac cgaacatttt tcaaagacca gctggttcgg ctaaatttgg ggttggaaaa
    30001 tcgtttatcc tcagaaatcg gtttgctgtc tggaggacaa cgtcaagcta ttacgttatt
    30061 gatggccaca ttacagcgtc cagaactgat cttattagat gaacacactg cagcgcttga
    30121 ccctgctact tccaaaacgg tgatggaatt gacagaaaag ctgatcactg agcagaatct
    30181 cactgctttt atggtcactc atgacatgga agacgcgatc cgctatggga gtcgactgat
    30241 catgctgcat caaggaagga tcgtcgtaga tatccagcaa gaggaaaaac agcagctcac
    30301 agtgccccaa ttattagcgt tgttcaaaca aaacagcggc acggcattaa aagacgatca
    30361 agtactttta tcttcataat cagaaagagg atgggacaaa aaatgaacgc ccaccctctt
    30421 cttttttctc tttccttctt tcgtttttgc tttatgtatc aaccgttgtt tcttcgaaaa
    30481 ctattttgtt ctctccttct tcctgataga atggatggga aaggagcttg atgccatgac
    30541 aacgaaagac aaggtacttc aattattgaa aaagacacca gattttcttt ccggagaaaa
    30601 gttggcacag caactggaag tttcccggac aagtatttgg aaagccatca aagaattgga
    30661 aaaggcaggc tatcgttttg agcacgggcc tacagggtat cgttacctgc cctctgacgt
    30721 actagatgca aaagaaatcc agcaagagct acggtcgttt attcctgatt tgaaagtaca
    30781 aacgatgttg acttctgaat ctacgatgaa agatgccaaa ctggcagcag ctcaaggaga
    30841 ttctgtttcg aagttatttg ttgctgatac acaagaacaa gctcgtggac gttttggacg
    30901 gccttttttc gctgaacctg gacgaggaat ctacatgagt ctcttgctcc atccagacaa
    30961 acggtttgat gaattgcctc aatatacgat catcgcagcc agtgcctgtg tgaaagccat
    31021 tgaatctgta accggtcgtt ctgcttcgat caaatgggtc aatgatatct acatggatgg
    31081 aaaaaaagtc tgtgggattt tatctgaagc agtcagtgat atggaaagcg gcaggatcag
    31141 tcatatcatc attgggatcg ggcttaattt ttctgttcct caaaatcatt ttccagctga
    31201 tctccaagaa aaagccatct ctgtctttcc cgacggacaa gctagtgtca caaggaatca
    31261 gctaatcagc aaaatctggc aagaattgtt cacttatctt tctcacccag aaaaagcgtt
    31321 ggacttctat aaagaaaaat cattcgtatt gggaaaaacc gtgtcgttca atcaaaaagg
    31381 aacgacttat gtcggtcttg caaaagaaat cggtgatcat ggcgaactga tcgtccaaac
    31441 aaatgaacag aagatgagtt tgtcttctgg cgaaatcagt ctcacatcta tccgtacgca
    31501 ataattatca gatttcacaa aaggacacct gatctcatac agtgtccttt tttctgaatt
    31561 ctcttcaaaa gactctcctt attctatcag ctgacagttt ctctatcctc tagaaaaagg
    31621 tattctaaaa atatctataa agcatggtac actttcttta aatgaatcaa tgcaaaaaac
    31681 aagagagtca tcagtttaca cgatcaaaag agatagcagg ccgcttcctt tttcaatctt
    31741 atagatagac catgggtaac gataaaaaca aaaaagaact tgcgacttat tttgccgtaa
    31801 gttctttttt agctagtgcc ttaatctctt aatcccaaca aacgtctcgc atagatcacc
    31861 tgaccagagt gcatagcagc atcatcaata atagaaacga gacgcattcc tcgtgtgact
    31921 ggtggtgtcc agttctcgtc aattacttca tctagctcgg aaggttccag ccttcttaag
    31981 taagaaatag caaaatcagc agctcctcgc aagtatccta gcagatcttc tatttgatta
    32041 gcttggatct gctttgcttg ttccagacta tgcttccaat cttgttcgtt atctgctatc
    32101 gtcatcggaa accggctttc ccagtcttga ctatgccaaa ttggagaagt tcctgctaaa
    32161 tcagcaatct ggaaatctaa ttctcgagcg gtatgccaag ccagccaagt cattgatttg
    32221 atctgcgggg ctctatcttc cactggaaaa cgattggcct cctcaataga gacgccttca
    32281 aacaaacgtt caaaccgatc gtatgcccga tccacactct cgcatagcat atctaaataa
    32341 tccatttatt ttcctccttt ttatttatcc tacttacttt ttcctaattc taggatacct
    32401 acttatatgt tactacacac ccttattttt tacactttaa cagtcaccat catcaatagt
    32461 ttggagattg aaagaaaaag atagtgtcga actatcaatt gaacaagaag caacaatatg
    32521 aacgtttcat taatattctt taaataaaca atgatttttc aaacgaacag tttaagacta
    32581 ggagaaaaag agtataatag gaaaatgttt gaaaggaaag gaaggggaaa aatgaaaaaa
    32641 agggcaagtt tactagtcct agttgctttg ttgttcaata tcttttcgta tggtgtggcc
    32701 gtttcagcgg aaacatacat tcctaaagac cctgggaata tttataatga tctaactggt
    32761 aactatggct tgttaggaat tgctagtcaa ttccacgtat ttgctaaaaa taagacaact
    32821 gtgaatgcac atacagacgg aaatatagca acacatgaac ttgatgcaaa aaataatttt
    32881 ggtacagagc ttcatgaagg tttattgaac caagaaataa attatataca aaaaactgat
    32941 tctttcgcta attcttcagg catacctaca ggtagcacaa gaatgaataa atttgtcgtt
    33001 ggcagtaacg tggatgtagc acttcaagat ggtcatccta cagtcaatgg atccaggctt
    33061 gatcacttaa ctccagatga tttttataaa gatcgtgctg ggcatgaata tattgatttc
    33121 gagcaggaat ttgctaaatt gaatgtagca gcaaatgatc ttgcaacgat cactccagct
    33181 aaaacatata ctgctgcaga ttttccagac atgaacaatc ggacaatcga tttaactgat
    33241 ttatcagata ccgggttttt attggtaaat atcgatgcag aagtgttaac gatgaatacg
    33301 ccacttcaaa tcattaatcc aaatgatcaa gtagttgtat ttaatgtcat caacagttct
    33361 tctgcattga acgtacagtc accaatcaaa tacaatgatc gttcaaatca tgaaacagaa
    33421 gatttttcgg atgcgaatat ctcttggaat tttggaaatg agatgactga tttgacaatc
    33481 agtgctcctt ttcaaggaac aatccttgcg cctaatgcta ctatccgcat tactcaaaac
    33541 caagacggaa cgattatagg aacaaatgtg attttagatg cagctaccaa tcgttgggat
    33601 cctaacgaaa tatttatcag cgacaatggt acagatacga cagacacaag cgacagtaca
    33661 gatacatcta ccacaagtga tagctcagat tcatca
//
LOCUS       DAYYCY010000011        33054 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000011 DAYYCY010000000
VERSION     DAYYCY010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33054
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.011"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            8..865
                     /locus_tag="ACHAZB_000432"
     CDS             8..865
                     /locus_tag="ACHAZB_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFM8613139.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            980..1900
                     /gene="pstC"
                     /locus_tag="ACHAZB_000433"
     CDS             980..1900
                     /gene="pstC"
                     /locus_tag="ACHAZB_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HFM8613140.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            1900..2784
                     /gene="pstA"
                     /locus_tag="ACHAZB_000434"
     CDS             1900..2784
                     /gene="pstA"
                     /locus_tag="ACHAZB_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFM8613141.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            2799..3605
                     /gene="pstB"
                     /locus_tag="ACHAZB_000435"
     CDS             2799..3605
                     /gene="pstB"
                     /locus_tag="ACHAZB_000435"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFM8613142.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            3621..4379
                     /gene="pstB"
                     /locus_tag="ACHAZB_000436"
     CDS             3621..4379
                     /gene="pstB"
                     /locus_tag="ACHAZB_000436"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFM8613143.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            4392..5069
                     /gene="phoU"
                     /locus_tag="ACHAZB_000437"
     CDS             4392..5069
                     /gene="phoU"
                     /locus_tag="ACHAZB_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFM8613144.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            5646..6338
                     /locus_tag="ACHAZB_000438"
     CDS             5646..6338
                     /locus_tag="ACHAZB_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFM8613145.1"
                     /translation="MLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISIL
                     FVVIGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYL
                     TRPYRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVI
                     GSMTNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPIS
                     VLFFLTVRRLKR"
     gene            6335..7102
                     /locus_tag="ACHAZB_000439"
     CDS             6335..7102
                     /locus_tag="ACHAZB_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFM8613146.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            7202..8770
                     /gene="liaX"
                     /locus_tag="ACHAZB_000440"
     CDS             7202..8770
                     /gene="liaX"
                     /locus_tag="ACHAZB_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HFM8613147.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            9067..9387
                     /locus_tag="ACHAZB_000441"
     CDS             9067..9387
                     /locus_tag="ACHAZB_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HFM8613148.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            9387..9737
                     /locus_tag="ACHAZB_000442"
     CDS             9387..9737
                     /locus_tag="ACHAZB_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFM8613149.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            9904..10842
                     /gene="hprK"
                     /locus_tag="ACHAZB_000443"
     CDS             9904..10842
                     /gene="hprK"
                     /locus_tag="ACHAZB_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HFM8613150.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            10857..11687
                     /gene="lgt"
                     /locus_tag="ACHAZB_000444"
     CDS             10857..11687
                     /gene="lgt"
                     /locus_tag="ACHAZB_000444"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFM8613151.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            11731..12756
                     /locus_tag="ACHAZB_000445"
     CDS             11731..12756
                     /locus_tag="ACHAZB_000445"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFM8613152.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            12771..13709
                     /gene="galU"
                     /locus_tag="ACHAZB_000446"
     CDS             12771..13709
                     /gene="galU"
                     /locus_tag="ACHAZB_000446"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFM8613153.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            13834..14274
                     /locus_tag="ACHAZB_000447"
     CDS             13834..14274
                     /locus_tag="ACHAZB_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HFM8613154.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            14277..14879
                     /locus_tag="ACHAZB_000448"
     CDS             14277..14879
                     /locus_tag="ACHAZB_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFM8613155.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(14938..16044)
                     /locus_tag="ACHAZB_000449"
     CDS             complement(14938..16044)
                     /locus_tag="ACHAZB_000449"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFM8613156.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            16254..17255
                     /gene="ccpA"
                     /locus_tag="ACHAZB_000450"
     CDS             16254..17255
                     /gene="ccpA"
                     /locus_tag="ACHAZB_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HFM8613157.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(17592..19937)
                     /locus_tag="ACHAZB_000451"
     CDS             complement(17592..19937)
                     /locus_tag="ACHAZB_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HFM8613158.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            20216..21118
                     /locus_tag="ACHAZB_000452"
     CDS             20216..21118
                     /locus_tag="ACHAZB_000452"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFM8613159.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAEKGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQTFDPEVFPELLAEEVIL"
     gene            21331..23106
                     /locus_tag="ACHAZB_000453"
     CDS             21331..23106
                     /locus_tag="ACHAZB_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HFM8613160.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(23172..24758)
                     /locus_tag="ACHAZB_000454"
     CDS             complement(23172..24758)
                     /locus_tag="ACHAZB_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFM8613161.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVSQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            25009..25233
                     /locus_tag="ACHAZB_000455"
     CDS             25009..25233
                     /locus_tag="ACHAZB_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HFM8613162.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            25375..27126
                     /locus_tag="ACHAZB_000456"
     CDS             25375..27126
                     /locus_tag="ACHAZB_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HFM8613163.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            27126..28910
                     /locus_tag="ACHAZB_000457"
     CDS             27126..28910
                     /locus_tag="ACHAZB_000457"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8613164.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFLAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     VLTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            29236..30087
                     /gene="licT"
                     /locus_tag="ACHAZB_000458"
     CDS             29236..30087
                     /gene="licT"
                     /locus_tag="ACHAZB_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFM8613165.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTITIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFGCVEKIADFLAETFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            30180..32066
                     /locus_tag="ACHAZB_000459"
                     /pseudo
     CDS             30180..32066
                     /locus_tag="ACHAZB_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
     gene            complement(32106..33008)
                     /locus_tag="ACHAZB_000460"
     CDS             complement(32106..33008)
                     /locus_tag="ACHAZB_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM8613166.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
BASE COUNT        10515 a         5874 c         6971 g         9694 t
ORIGIN      
        1 atttctaatg aaaaaactta aattattctt accaataata ggattagcta ttcttttatc
       61 aggctgttca aagtggattg atcgaggaga atccattacg gctgttggct cttctgcttt
      121 acagccttta gtagaaacag ttgctgaaac ttaccagaca gaaaatcctg gaaaattcat
      181 caatgtacaa gggggcggaa gcggaactgg tttgtctcaa gtacagtcag gagcagtcga
      241 catcggaaac tctgatttgt ttgcggaaga aaaatcgggg atcgatgcct cagcattagt
      301 cgatcatcga gtagcagttg ttgggatcac tcctattgtg aacaaggata ttggcgtaaa
      361 agacatcagt atggaaaacc tgaaaaaact atttacaggc gagatcaaaa actggagtga
      421 agtcggtgga aaagatcagc cagtagttat attgaaccgc gcttcaggaa gcggaacccg
      481 tagcacgttt gaaaaatggg tattggacgg agaaacagct gttcaggcac aagaacaaga
      541 ttcaagcgga atggtgcgcc agatcgttgc cgacacaccc ggtgcaatca gctatgttgc
      601 tttttcttat gtttccgatg atgtagatac gttgaatgtt aacggtgtag aaccgacaga
      661 tgaaaatgtg acaacgaaca aatggacgat ttgggcatat gagcatatgt acacaagaga
      721 tcatccaaaa aaactgacaa aagaattttt agactatatc ttatctgatg aagtacaaga
      781 taacatcgtt ggagaactag gttatatccc tgtttctcat atgaaagtcg aacgtgattg
      841 gcaaggaaat atcgttaaag agtaatcttt acacaaaatt tacaaactta gctaaacttt
      901 tacatgttat taaaaccaaa ttgatgttca atttgtacac tgagtgagta tgttaaacct
      961 gattaagggg gagtcatctg tggaagatgt gaaaaaagta ttattaacaa aatcaaaacg
     1021 ttcaaaaatg gaacagcgtg gaaaattcat cagtttttta tgtatcgcgc tgatcgtgtt
     1081 ggttgtatta tctatttttt attttgtagc tagtaaagga ttggccacct tttttactga
     1141 taagatcaat gtatttgatt ttctgtttgg tacagattgg aatccaagcc aaatcggagc
     1201 aaatgggaaa ccaatggtag gggcattgcc tatgatcaca ggttcgttta ttgtgacctt
     1261 tttatctgct atcgttgcca cgccatttgc cattggtgca gcagtattca tgacggagat
     1321 ctctcctaaa aacggaacga agatcttaca gccggtcatc gagctgcttg taggtattcc
     1381 ttctgtcgtt tatggtttta tcggtttatc agttatcgtg ccttttgtca gaagtatttt
     1441 tggcgggact gggtttggta tcttagctgg tacatttgtg ttgttcgtta tgatcttgcc
     1501 gactgtcaca acgatgactg ttgatgcatt aaaggcagtg ccacggcact atcgggaagc
     1561 ttcattagcg cttggtgcca caagatggca aacaatctac aaagtcgtgc tacgagcagc
     1621 cgtgcccggg attttaacag cagtcgtttt tggtatggct cgtgcgtttg gtgaagcctt
     1681 agctatccaa atggtcatcg gtaatgcagc attgatgcca acaggtctta ctactcctgc
     1741 ttctacattg acgtccatct taacaatggg aatcggaaat acaatcatgg gaacagttga
     1801 aaataatgtg ttatggtcat tagcgctgat cttgctattg atgtcattat tctttaatat
     1861 cgtgatccgt atgattggga agaaaggaga gctgaaataa tgaacgcaaa acgctcagat
     1921 aaaattgcta caggtgtttt atatatagtt tcggggatca tcgtattgat tttagcagct
     1981 ttactacttt atatcttagt tcggggaata ccgcacattt catggtcctt cttgaccgaa
     2041 ccttccaaag cctaccaaga aggcggtgga atcgggatcc agctatttaa ctcgatttat
     2101 ttgcttttga tcacgatgat catcagtttt cctatttctt taggtgcggg tatctatctt
     2161 tcagaatatg ctggaaaaaa ctggctaaca gatgtgattc gcacgtcgat tgaaatcttg
     2221 agctcgttgc cttctgtcgt tgtcggttta ttcggtttct tggttttcgt tattcaattc
     2281 cagtatggtt tctcgattat ttctggtgca ttggctttga ctgtattcaa cttgcctctt
     2341 ttgactagaa cagttgagga gtctttacaa gcggtccatt acacacaacg agaagcagga
     2401 ttggccttag gtttgtcacg ctgggaaaca gtgataaaag tggtggttcc agaagcgaca
     2461 ccagggattt tgactggtgt gatcctaagt tctggaagaa tttttggtga agcagctgca
     2521 ttgatctata cggctggaca aagtgcacct gcgttagatt ttacgaactg gaatccatta
     2581 agtgtttcaa gcccgatcag cattttccgt caagctgaaa cattagctgt ccatatttgg
     2641 aagatcaata cagaaggcac gatgcctgat ggtgtttctg tttcggctgg cgcttctgcc
     2701 gtgttgatta ttgctgtttt actgttcaac ttcggcgctc gtgcgattgg caaacgatta
     2761 taccgaaaaa tgacttcagc ctaaggagaa gaagcaaaat gaaaaaatat aactggaatg
     2821 aaacaaatgt catcaccatg ccaaaagaga aagaagtagc actatatacg gatgatctac
     2881 atgtctggta tggcgacaat gaagcaatca aaggagtgga tctggaattt gaaaaaaata
     2941 agatcactgc actgattggt ccttccggct gcggaaaatc tacgtatctt cgttcgttga
     3001 atcgaatgaa tgatgggatc gctaatacga aaatcactgg gaaaatcatg tatcaaggcg
     3061 tagatattaa tgcgcctcaa gtggatgttt atgagatgcg caaacggatt ggcatggttt
     3121 ttcaacgtcc taatccgttc agcaagtcga tttatgacaa tattacattt gctttgaaac
     3181 agcacggcga aaaaaacaaa aagaaattag atgaaatcgt tgaaacaagc ttgaaacaag
     3241 ctgccttgtg ggaccaagta aaagacagct tggacaaaag tgccttggct ttatctggcg
     3301 ggcaacagca acgtttatgt attgctcgtg cgatcgcgat gaaacctgat atcttgctgc
     3361 tagacgagcc ggcaagtgct ttggacccta tttcgacagg aacggttgaa gaaacacttg
     3421 taaacttgaa agaagactat acgatcatca tcgtgacgca taacatgcaa caagcagctc
     3481 gtatcagcga ctatactgcc tttttctatt taggaaaagt aatggaatat gacaaaacta
     3541 acaagatatt taccagacca aaaattcagg caaccgaaga ttatgtgtcc ggtcattttg
     3601 gttaagacgg aggcctcaac atgacaaaag agattatttc atctaaagat cttcatctgt
     3661 attatggaaa gaaagaagca ctaaaaggga tcgatctaag cttcaatcaa ggggaaatca
     3721 ctgcgatgat cggtccttct ggttgcggaa aatccactta tctgcgttct ttgaaccgga
     3781 tgaatgacct gattccgaat gtaacgatca ccggaagtgt tttatataaa gacaaagaca
     3841 tctatggtcc aaaaacagat gttgttgaac taagaaaaga aatcggcatg gtattccagc
     3901 agccaaaccc atttcctttt tctatctatg aaaatgtcat ctatggattg aaattaaaag
     3961 gggaaaaaga taaaaaagtc cttgatcaag tagttgaaga aagcctgaag gcagcgtctg
     4021 tttgggatga tgtgaaagac aaattgcata aaagtgcttt atctttatcc ggtgggcagc
     4081 agcagcgtgt atgtatcgca cgtgttttgg cagttaatcc ggaaatcatc ttattggatg
     4141 aaccaacaag tgcgcttgat cctgtttcta ctgggaaaat cgaaagtatg ctgttagaat
     4201 taagagagcg gtatacgatg atcatcgtga cccataatat gtctcaagct tcacgtattt
     4261 cagataagac ggcattcttt ttagatggtc acttgatcga atacaatgat acgaagaaga
     4321 tattcatgaa tccagaaaaa caagagacag aagattatat ttcaggtcga tttggataag
     4381 gaggaaacgg aatgctacgt acacaatttg aagaagaatt gctgaatctc cataatcagt
     4441 tctacgaaat ggggatgatg gtgagcagcg ccgtgcacaa gtcagttcgt gcatacatca
     4501 aacacgataa aatcttagcc caagaagtta tcgaaaacga tataaatatc aataacatgg
     4561 aaacacgctt ggagaagaaa agttttgaaa tgatcgcatt gcaacagcca gttacaactg
     4621 atttgcgtat gatcattacg gtcatgaaag ctagctcgga tttagaacgt atggccgatc
     4681 atgcggtgtc tgttgctaaa tccacgattc gcgtaaaagg gcagacgaga ataccggaaa
     4741 tcgaaaaaga gatctccgat atgtccgatt atgtgaaaaa aatggtggac aacgtcctag
     4801 ttgcttatgt caaaacagac gaaaaagacg cacgtacgat cgccaatatg gatcaaagag
     4861 tcaatgacta tttcaatcga atctataatg caacaatcaa aaatatgcag gaaaatcctg
     4921 agacagtcat cagtggaaca gactacttga atgttgccag ttatctagaa cggatcggag
     4981 actatgtgac aaacatctgt gaatggatcg tctacttggc tacagggaaa atcactgagt
     5041 taaatacgaa ccacaatgag attttctaaa ctgaagctgt gattatgccg tttgtttagg
     5101 aaaagaggtt gtgaaatcag cggttgtgaa aaagctgttc tgcatcaagt aataagaaca
     5161 gtcaaacaaa aaatagctcc tcatattttt cgtttggctg gacttattac cgcagatgca
     5221 agcttttgaa caccgtttat tcgggaaagg gtttgtgaca aaacatgtca caaacccttt
     5281 tcatcatttc atagaaagga taaagaatgc aaaaaaaaat tagcatcatc actatatttt
     5341 ttatttgtgt gcttacttca tgggttattc agcatcaagc cgctttatat gaagtacctg
     5401 tcatcgagat agaaaaagta cgaataaaag aggatcatca attgattaca ggaaaactga
     5461 tgaacacgga tcaaaaagga gaatcgattt atttatcggt tccttatcaa aaaaatcaaa
     5521 tagaaacagc aaatataaaa gcgagacaaa tgtatctagt ccaaatgcat ggagatgaat
     5581 ggcagctagt agcaaaaaaa aatgatggct ggcttttctt cttcacaagt atcttcattg
     5641 gcgccatgtt gcttattggt ggaaaacagg gtctatttgc tttaggaagt ctcatcatca
     5701 atatcgtttt acttttactt ttgttgctca tctttactaa aacagaaggg atttctctat
     5761 taaatatatc tatcctgttc gtcgttatag ggatgattat tagcatattg gctttagatg
     5821 gatggaatag aagcagtata atcaaaattt cagctgcagt tacgagtgtt tttttagcat
     5881 tttttatttg tcttttgaca atgaatcatt ggaaagacca aggactgcgc tatgaggaac
     5941 tgaattatct gacacgaccg tatcgttctg tttttctttc tagcgtactg attggaacag
     6001 ctggaggaac actcgatacc gttatcacag tcattgcgac attagaagaa ttgactaagc
     6061 gacaaccaga aataacagcg aaacagttat ggctttcagg aaggcaagtg ggtcgagatg
     6121 tgattggctc gatgaccaat atccttttgt tcgtctatat cagtgggtca gttccatcgc
     6181 ttcttttata tttaggaaat caatggtcgt tcaaagaaac gatagaacag catttatcat
     6241 tggaattttt acgcgtcatt gctggcagct taggaatagt attatcgatc cctatctcgg
     6301 tcctcttttt tctgactgta aggaggctga aacgatgaat gtacttatta tacttgttgg
     6361 gattttagca atcagcgtgc tacttattgg cggtacgcaa ggtatgtata ttcttttagg
     6421 cttattcatc aatttaggga tctttttcct gttgttattc ggttatcatc agaagtggcc
     6481 gatactggca ttgtcggtca taggattttt attgattgct gtggttatct tattttttat
     6541 caacggttat aatctaaaaa tgcgtgcagc ttttgtgagt atactgatct ttttattttg
     6601 tttcctacta ttgcttccta tcactgattt tttagcaatc caaggtttta catcggtaga
     6661 actagaagaa ttatctggct tagataagac tttagcgatt gattttagat tacttacgag
     6721 atcgttgctt ctgattagtc ttagtggtgc tgttttagat gcgagtgtag caatttctag
     6781 tgggacattc gaagtatatc aggcaaatcc gcaattatct ttttaccaat tgcgccattc
     6841 tagtttcgct attgctaaaa aaatattggc gtcaacagta atgacacttc tttttgcatt
     6901 catgggaagt tctttagcgt tgatcctctg gtttattgat ttagacattc cttttcagca
     6961 agtgatcaat gaaaaatcat ttgttctaga atatacgatg gctattctta ccacattgtc
     7021 cgcactactt gttcttcctt taacctgcgt gactacagca tatctcttta caaaaaagaa
     7081 agaacaactc aagatggagt aaaagttaca acttaagtcc tatgacattt ttactgaatc
     7141 agtgcataat atggatgtaa ggaaaagctt gcaaacaatt ttgcttaagg aggcaatttc
     7201 catgaaagaa agagaacgta ttttagattt agtgaaaaaa ggaatccttt cgactgaaga
     7261 aggcttagat ttattagaaa gcatggcaac tgaaaaagac gaaaagcaaa tcaaaaaaga
     7321 agcagacaag gtaactgcta gtcataaaga aaaagatcag gcaagtcaat tgatcgataa
     7381 acttgaaaac ggcgaagaag aaatttctga gccagttgat ccaaaggaaa gagaaagaca
     7441 agatcaagaa aacttagaaa agatcttaga tgaactagca actgaagcga ataaaacttc
     7501 ggcgcgtcta gatgaagtaa atgctcaatt cgctgacaat aaggcagcac gcaatgagaa
     7561 acaggaagca ttgatgcagc tgaatacaaa agaagaatta ggcgagttga cagaagaaga
     7621 attagcacag cgtcagacgt tagaagcaga aatcaaagaa ttagaagcta gtggcgatac
     7681 actgttagaa gaacaattga aattagaagc agaattaaaa gatatccgta agaatcaatg
     7741 gtctgagaaa aaagaaacgt ttactgataa gtttgaattg ccagatgatt ggaaagatca
     7801 agcaacagat acactgaatc aagtaggcga aaaaatgtca gaagctggat cacagttagg
     7861 caaattcttg aagaaaacat tccaaactgt ttcagaaaca gtgaatgaca acatggaatg
     7921 gaaagatgtc agcttacgtg tccctgggat tgcgacaacg aaatttgagc atgaatttta
     7981 ctatgaagct cctgcagcaa gtatccttga tatcaaggca gcaaacggaa acgtgacgtt
     8041 aaaaacgtgg gacagcaacg atgtgaaagt tgaagctaag atcaaacttt acggaaaaat
     8101 gggtgctgaa ccatttgaag cattctctga acggagccag attgaggtta atgaagatca
     8161 tatctccttc caaattccaa ataaacgtgt gcgtgcagac cttgttttct acttacctaa
     8221 acgagtatac gaccacgcag ctattaaact tctaaatggc aacatcatga ttgaaacatt
     8281 ggaagctaaa gatatttata caaaatctac gaatggtaac atcatcgtgg accaattaac
     8341 tgcaacgatg ctggaagtag aaggtgtaaa cggcaatatc gatattcgaa acgggaacat
     8401 cttagattct attatcgaaa cagtaaatgg tacagtgaca tttggtgcga cagcagaaaa
     8461 cctttctgtg tctttggtaa atggtgatgt acgtcttacg attaaagaag ataatttgaa
     8521 aaaagtagaa gcaagttcag tgaacggaaa tgtcaaagtc gctttaccag atggcatcgg
     8581 tttggaagga catgctaaaa caagtcttgg aagtatcaac agccgtctgt ccaattacga
     8641 agtagttcgt gagaaaaaag aacggacaaa ccaaatgttg caattccgtc gtgtttcaga
     8701 tggtgaaatc gcgcaagtgc aactttcaac gacaacagga agtatctatt taaaagatac
     8761 agataaataa agaggttgtg aaagagatgt tcagccttga gcaataagaa gcgattttgt
     8821 aaaatagctt cccctatttt acaaaattgt gccttattgc cgatagtcta tctcttgaac
     8881 accgtttacc aagagattat gaacctgcca attatcgccg agtcacaagg aataatttca
     8941 gaaaaagctc gtcacgtttt atgaatatgg taatttgtga ccgagaattt tgcagaaaga
     9001 caccgattaa gaagaaggtt gtcacaacga ttttcttcat ttaaaatagt agaacagagg
     9061 tgagacatga tgaaaaaaaa gttgacaaaa tctaataaaa atgttgtttt gacagggact
     9121 ttagcaggaa taggagaata catcgggatt gatccgactg ttatccgtgt gctttatgta
     9181 ttcctgagct tagttgcttt tggaagtcca attattttat acattttatt agctttgatt
     9241 atcccatctg cgcgttctgc acgaacaaac tatggacata acaatgcata ttatgaaaaa
     9301 aacaattatc gtgaacaaac tcgtgaacaa acaaaaaaac gcaaagaagc ggaaaaaatc
     9361 gatgatgacg aatggagtga cttttaatgt cgtactttca acgtatttta gttaatacgt
     9421 taacattcat atcattggct gttctttttc ctgcgatggt ccatgtgagc agtatttgga
     9481 cggccgttct tgccagcttc gtattgtctg tgttgaatat gtttgtcaaa ccgattttga
     9541 cgatactgtc cctgcccttt acattagtca cattgggatt gtttacgttt gttatcaatg
     9601 cagcgattct taaaatgacc tccttttttg tgggagcaga acattttagt ttttcttcat
     9661 tcggtgcagc gttgttagta gctatcttga tgtcgcttgt caatgtaatt gtcagcgaac
     9721 ataacagtag aaaataaaag aaagctgtgc cgaactatcg gcgcagtttt tttatttaga
     9781 atcaggaatg cgttttctat tacattggta attaaataaa aacgtttttt ttaattggta
     9841 gtacttttgg ctagacaatg ataaaatgat agagaacact ctaatagaaa tgaggtagca
     9901 tgaatgtcag aagttgtaaa agttagccaa ttagtgaaaa aattatcttt ggaaatcgtg
     9961 acaggcgacg agaaaagctt agaccgtgtc atcgtaactg gggacatctc acgcccagga
    10021 ctagagttga ctggttattt taattattat tcccataatc gtttacagct atttggcagt
    10081 aaagaaatca gttttgcgga acggatgatc ccagaagagc gtttgatcat catgagaaga
    10141 ttatgtagtg aagatatgcc cgcatttatt atttcaagag gattaccagc tccagaagaa
    10201 atgatccagg cagcaaatga aaatgggata gctgtactgc gttctcccat ctcaacttct
    10261 cgtttattag gagaattatc cagttattta gatggtcgcc ttgcccctcg aacaagtgtc
    10321 cacggtgttt tagtggatgt ttacggatta ggtgtactga ttcaaggtga cagtgggata
    10381 ggaaaaagtg aaacagcgtt ggagttgatc aaacgcggcc atcgattgat cgcagacgat
    10441 cgagtagatg tttatcagca agatgaatta acggtgatcg gagaaccgcc taaaatcctg
    10501 gaacatctga ttgaaatccg aggaatcggg atcattgatg taatgaatct ttttggtgcg
    10561 agtgcggtac gtggctctat gcaagtccag ttggctgttt atctggaagc atgggcaaaa
    10621 gataagaaat acgaccgtct gggctctgaa gacacgaccg ttgaaatcgc ggaagtgggc
    10681 attccacaaa taaaaatccc tgtcaaaaca ggacgaaacg ttgcaatcat cattgaagta
    10741 gccgcgatga atttccgcgc acgtacaatg ggctttgacg caacgaaaac atttgaagaa
    10801 agattaagtc gcttgattga agaaaactca ggcaacgact agcaaggagt gacaatatgt
    10861 tagcacaaat caatcgagtt gcttttgagc ttgtagggat tcctatctac tggtatgcat
    10921 tgatcatcgt atcaggtatc attatcgcta tgtggcttag cagcagagag gctgttcggg
    10981 taggattaaa agcagatgat gtgacagatt ttatgttgtg gggattgcct atctccatta
    11041 ttggcgctcg cttatattat gttttatttg atcttcccca gtatatagct gatccaatcc
    11101 aaatattcaa tatccgttca ggaggcttag ctatttatgg cggactgatt gcaggcgcga
    11161 tcacgctgta tttttttact aaatatcatt ttatttcttt gtggactttt ttagatatcg
    11221 ctgcaccaag tgtattatta gcacaggcaa tcggtcgttg gggaaatttc atgaaccacg
    11281 aagcatatgg accagacacg acgcgaagct tcttagaaag tctgcattta ccgcaattca
    11341 tcattgataa tatgtatatc gaagggatct atcgtcaacc aacatttctt tatgaatcac
    11401 tttggagttt gattggattt attttgcttt tgtttttacg aaagaaacca caccttttga
    11461 aaaaaggcga agtcctttta tgttatctaa tgtggtatag tttcgggcgt ttctttattg
    11521 aaggattacg tacagacagt ctttactttt taggtgtgat ccgtatttct caattattat
    11581 ctgctgtgtt atttgtcggt acattgattc tatttatttg gcgcagaaaa aaaggaaatt
    11641 taccggatta tgaccgctca ttaggaaaaa atcaacaaat tatctgattt ttagagcgtt
    11701 aaaaatgaaa aaaatttcag gagtggtaaa atgaattata gagtagcggt tttaggacca
    11761 ggctcttggg gtactgcttt ggcacaagtc ctcgccgaaa acggacatga cgttcggatt
    11821 tggggacatc gtgcttcaca agtagaagaa atcaacaccc aacacacgaa tcaacgttat
    11881 ttaccagatc attatctacc cgattccatc caagcattca ccaatatgga agatgcagta
    11941 aaagacgcag atgcggtgct attcgtcatt ccaacgaaag caatacgttc tgttgcaaaa
    12001 gaactagtgc ctaaattgaa tacaaagcca gtgatcatcc atgcaagtaa aggattagaa
    12061 cagggaacac ataaaaggat ctctgacatc ttgttagagg aaatcccagc agaaaaaaga
    12121 aaagctgttg ttgttctttc tggaccaagc catgcagaag aagtagcaaa acatgacatc
    12181 actacgatca cagctgcaag tacagatgca aaagcagcaa aatatgtaca agagctgttc
    12241 atgaataatt attttcggat ctataccaat cctgatgtga tcggtgtaga gatgggggca
    12301 gctttgaaaa atattattgc tattggtgct ggcgctattc acggcttagg gtttggcgat
    12361 aatgcaaagg cagcaatcat gacgcgcgga ttagctgaga tcagccgttt aggtgtagca
    12421 atgggtgcca acccgctgac ctttatcgga ttaagcggaa taggcgatct agtcgttact
    12481 tgcacaagtg ttcattctag gaactggcgc gcaggcaact tgttaggaca agggcagccc
    12541 ctagaagagg tgctagccaa tatgggaatg gtcgttgaag gtgtgaatac tacccaagca
    12601 gcgatggaac tagcccaaat gatggaaatc gaaatgccca tcactcaaac aatctatgaa
    12661 gtgctgtatg aacaaaaaga aatcaaaaca gccgcaaaag aaatcatgtt acgtgatgga
    12721 aaaaaagaaa atgaattctc attaacaatt ttttgaagga gcgaatgtat atgaaagtta
    12781 aaaaagcagt tattccagca gcaggattag gaacaagatt tttaccagca acaaaagcta
    12841 tggcaaagga aatgttgcca attgttgata aaccaacaat ccagttcatc gttgaagaag
    12901 cgttgaaatc aggtatcgaa gacatcttga tcgttacagg aaaagctaaa cgaccaatcg
    12961 aagatcactt tgattcaaat attgaattag aaacaaacct aagtgaaaaa ggaaagaccg
    13021 aattattgaa attagtagaa gaaacgacag atgtcaactt gcatttcatc cgtcaatctc
    13081 atccaaaggg tttaggacat gccgttcttc aagcaagagc ctttattgga aatgaaccat
    13141 ttgtcgttat gctaggcgat gatataatgg aagacgaagt accattgaca cagcaattaa
    13201 tcgatgatta cgaacgtact catgcttcta cgatcgccgt tatgcaagtt cctcatgatg
    13261 aaacttcaaa atacggaatt atcaatccag gagaagtatt ggaagatggg ttatacaatg
    13321 tcaaaaactt tgttgaaaaa ccagatccaa gcaaagcacc aagtgattta gctattattg
    13381 gacgttactt attgactcca gaaatttttg atgtattgtc cgagcaacaa ccaggagccg
    13441 gaaatgaaat ccaactaact gacgcaatcg atacattgaa taaaacacaa cgcgtatttg
    13501 cccgtaaatt cacaggtaaa cgttacgatg tcggtgacaa atttggtttt atgaaaacaa
    13561 gtattgaata tggtctaaca catccagaag taggtgcacc tttacgtgag tacatcctgt
    13621 ctttaggtaa aacattagaa gcagaaggaa gcaagatcga tcataatcca aaaggaataa
    13681 cagaaatcaa agaagaaaag aaaaaataac ccttttttct aacgagcttc agctgctaat
    13741 tgaataaaaa gtagttgaag ctcgttttta tttgtttaaa aaacacgaaa atgctatcct
    13801 tatagaaaag agagacaaga agggagtttg atcatgtcag gaggagaaat tgcaggattg
    13861 atcgcagcaa tcgcctttgc gatacttgtc gtttttatcg ttcgtgtgtt gttgcaagtt
    13921 tctaaaactt tagaaaaagt agatcagact gtagcagaag caaatacgac gatcgaaatc
    13981 gtcacaaaag acgtagatct tttgtctaga caagtagaag gcttactggt caaaagtaat
    14041 gaactgctct tggatatcaa tggaaaagtc gcaacgatcg atccattgtt cacagcagtt
    14101 gctgatttaa gtgaaagtgt atcagaactt aatcattcta gtaaaaacat cgcgacgaaa
    14161 gtcggcggaa tcggtaaaac gacagctaaa gcgacggttg ccggaaaagt cggtgggaca
    14221 gcgatgaaat tttttaaaaa caaaaaacat actgaaacga caggaggaaa ataaaaatgg
    14281 ctaaaaaagg tggattttta ttaggtgcag tagtcggagg cacagccgca gcaatcgccg
    14341 cgttattgtt agcaccaaaa tctggtaaag aacttcggga tgaattagct gcccaagcgg
    14401 atgatttgat ggatatggct tcagattaca cagatatggc gaaagaaaaa tcgactgagt
    14461 taacaggtat tgcaaaagaa aaagcgcaag atctttctca acaagcaggt gatctagcag
    14521 gtagtgtcaa acaaaaagta agtgaatcag cagatgaagc aggaagtgaa tctgacgata
    14581 tcttgttaaa tcttaaagaa caatcagctg acttatctaa tcgtttcaaa aaaacggcag
    14641 aagatatgaa aaaacctgca gatgaattag gaagtattgc taaagatact tcagaagata
    14701 tcatgattga tgtaaaagac gctgctggta aagcaggaga tactgtcaat gaaggaatgt
    14761 cagaagcaaa accagttgca gatgactcaa aagaaatgat gaaaatggca aaagaagatg
    14821 cgaaagaagc taaaaaagaa ataaaagata caatgacaga ttcatctaca aatcactaaa
    14881 atcacaaatt tatagaataa aaaggtggga gcggactagc tctcaccttt tttactctta
    14941 tttttctaaa acaagtaatt cttttggtgt agtcgtgaat ggcacacagc catcgcttgt
    15001 cacatataca cagtcttcga tacgtacccc tactttttca ggaatataga ttcctggttc
    15061 aagagagaag cacatccctt cttcgattac taagtcattt ccttctacaa gggaagggaa
    15121 ttcgtggacg gttgttccga tcccgtgacc taaacggtga ttgaaatatt cgccgtagcc
    15181 atagctgttg atcacgtttc gtgcgatttg atcaagttca cctgctgtca cacctggacg
    15241 gacggcttct tgtgcggcaa gctgtgcttc aagagtgatg ttgtagattt tttcttggaa
    15301 gtcggttggt ttttgataag caattgtacg agtagcatca ctgcaatagc cattccaaac
    15361 aacgccaaga tcgaataaca caagttcatt cggtgaaatc ttcgtatttc ctggtgtacc
    15421 atgagggctt gcagcatttg ctcctgcaag gactaatgtg tcgaaagaca tttgtgaaac
    15481 accttgtttt ttcaattggt attcaatttc tgcaatgatc gcttgttcag tcacgccttc
    15541 tttcactgct ttaaagccaa tctcaaatgc aacatcggcc catttaccag cttcgatcag
    15601 tttgtctttt tcctctagag ttttgatcag ttgtagacgt tggatgatgg aagttagatc
    15661 agtgtaagaa tccgttcccg tcaagatatc agagatagca tcgaagcgct cgacagtcaa
    15721 ggcgttcttt tcaatcccta cattgccagg ctgaccatag cgagcacgaa gttcttttcc
    15781 gatgatctcc caagggtttt cgctgtctaa atagccacgg acatcatacg cccaactact
    15841 gttttgagca tcttcaactt ccaaagcagg agtgaaaagg aaaggatctt gttcaagagg
    15901 gataaaaaga gctagtacac gctcatgagg atcactttcg tatccggaaa agtaagcgat
    15961 gtggccaggg tcgctgatat aagccaattc gacttggtgt tgtgccatcc attgacgtag
    16021 ttcattgatt tttacgttat tcatttggca acctactttc taaattaatt ttgtattcaa
    16081 taattgtacc actgatttat taaaagacca acgttctaaa aactgaaaaa agtaaaaatt
    16141 ttcatgaaaa cagagataaa acggttgcaa ttcttgagat tatttgatat tctattttag
    16201 aaatgtaaga cgtattttca gaaaacatat aaaatatgaa taggagaaca caaatggaaa
    16261 agcaaacaat cactatctat gatgtagcga gagaggcaaa tgtttcgatg gctactgttt
    16321 cacgtgtcgt aaatggcaat ccaaacgtta aaccagcaac aagaaaaaaa gtcttggaag
    16381 ttatcgatcg cttggattat cgtccaaacg cagttgcgcg tggcttggca agtaagaaaa
    16441 caacaacagt tggtgtaatc atccctgatg tcagcaatat gttctttgct tctttagcac
    16501 gtggtatcga tgatgtggca acaatgtaca aatacaatat tatcttagca aattcagatg
    16561 gcaacgacca aaaagaagtc aatgtattga ataacttact ggctaagcaa gtggacggcg
    16621 tgatcttcat gggtcaccat atcacagacg aaatccgtgg agaattttca cgttcaaaaa
    16681 ctcctgttgt attagctggc tctattgatc cagatgaaca agtaggtagc gtaaacattg
    16741 attatgtatc cgcaacaaaa gatgcagtaa acaaattggc aaaaagcggc aataaaaaaa
    16801 tcgcttttgt cagtggtgcg ttgatcgatc ccatcaatgg acaaaatcgt ctgaaaggct
    16861 ataaagaagc attaagcgaa aataacctac catatagcga aggattgatc tttgaagcac
    16921 aatacaactt caaagatggt ctatctctag tcgatcggat ccataatagc ggtgcaacag
    16981 ctgtctatgt ttcagatgat gaattagcca tcggtatttt ggatggttta ttagaccgtg
    17041 gcgtgaaagt accggaagaa ttcgaaatca tcacaagtaa caattcacta ctgacagaag
    17101 tagctcgtcc tcgtttaaca agtatcacac aaccattata cgatttaggg gcggtatcta
    17161 tgcgcctatt gacgaaaatg atgaataaag aagaagtcga agaaaaaacg attatcttgc
    17221 cttatggaat cgaagaaaaa ggatctacaa aataataaaa ggacaaccat tcagaggttg
    17281 tgtgcctgcc gtttaactct gcgttacaag gagcaatttc aaaaaacagc atctcatgtt
    17341 tgatgaatat tgtgactcgt gattcggagt tggcaggaga acaccgttta tttgggaaaa
    17401 ggggttatga caaaattttg ttataatcct ttttttgtgt cttaaaaaag agagaaagtg
    17461 gttgaaatta catttatcgt atataaaaca atccgtgtat agatgctgca ttttgttcac
    17521 caccatcaaa aaaataaggg actgtgacaa tactattgtc acaatccctt attactgtgt
    17581 attctattaa atcaatcatc tttctcatct gattctttag ttgcttgatt cgctcttgca
    17641 taagctgaag cggtgttcca gtaatctgta tagttttcat cggtaccgcc aacgccaaat
    17701 ttaaaggcgc ttttcttcgg tccatttttc gcccatagcg attcaactgt ttttcctggt
    17761 gtttggatgg attttttatt gacagtaact tttccaggtg tttgaccagt aaatccagat
    17821 acttttttct tcatcacatc agatgataat tcgaatttct catcagtgcc aaaaatgtca
    17881 ggttttgctt ggtaagcttg attgatcaga ttggcaagat aattagccga acgtatcctt
    17941 gctttgctgt ccattccagt attgtcatcg tgccctgccc agctactgat ggtaatagac
    18001 ggagtagaga caattagcca tgaatctcgg tattcattgg tagaaccagt ttttccgacc
    18061 caatctgctt ttcctagatt tccgtttaag cttgagatgg catctttgaa tggtgtagtg
    18121 atttttgcat tgatcacact acgcatcatg tcattcatga tcgatgccgt tgcttccgaa
    18181 tagatccgga cagagttgct ttcgtgttta taaattacat ttcctgcgtt atctgtgatg
    18241 gaatcgatga tatagccttc ttcatagacg ccgccgtttg ctaaagcctg aaatccgttg
    18301 gtctgttgaa gcgtcgtgac atctactgta cctaaaggag cagattcaac gccccaattg
    18361 tcatttgccg ggtagttcat ctttgccaag tagtgctcgt aagcatattg tttcgatcca
    18421 ccggaattca acacatcttg atataagtga taagccggga tattgttgga ccattctagt
    18481 gactcacgga cggtttggaa ggtgttcgat ccttcgttag tagcattgac gattttctcg
    18541 ccagcatttt ctccttcttg ccaagttgtt gcataatctg atacacggga ttctgatccg
    18601 ataagcccca tgtcgattgc aggtccataa acaagaactg gtttgattga tgagccagct
    18661 tgacgttctg tatcgaaggc atggttattt tgattctgac tgtaattccg tccgccaacg
    18721 aatccgtaga tgcggccagt tctgttatcc atcaatacat ttccggtttc tacttgactg
    18781 ccagcaccat catctaacat gtatccataa ttttcaacgc cattttgcaa tgcagcatag
    18841 acatctttat cgattgtcga atggattgta tatcctttat tttggatcgt ttgctgtgcc
    18901 ttttgataat aagcgtcact gaccgatgaa tcagacatat cagcgttgtc tttttcagcc
    18961 aattgttgtg caatgattct tgttgcttca ttcatcacag tataatacaa gaattctctg
    19021 tcagtctgtt ctgcgatttg ttgtggtaga aagtcttttg taaggtcata ggcttttgct
    19081 tcttcgtatt gttctttcgt aatttggcct tcacgataca tactgaataa aacaaaatct
    19141 tttctggcaa ggccggcaga aagatcttct tttagcgcgc ctgtgttcgt gtatggacta
    19201 tacgtgatcg ggctttgggg taatccagcg atataagcag cttgcggcag cgtcacatct
    19261 tttgcatcta caccaaagat tccttttgca gcttcctgga ctccagcaat gttttgtccc
    19321 ttattattac gaccaaatgg tgagacattc aaatacgtag caatgatctc atcttttgaa
    19381 aagtattttt ctacttgtgc agaaagaatg atctcgtttg ctttacgttt gaatgttgtt
    19441 tcatctgtaa ggatctgctg tttgactaat tgctgtgtca aagttgatcc accagaagag
    19501 ccaataccag tagcttcaga tactaatgct cgtaaaacag ctttaggaac gtaaccttgg
    19561 tgtttatcga agtattcatc ctctgtgctg ataattgcag ttttcaataa aggtgaaatc
    19621 ttatcagatg aaatagtcgt gcgcatcaga tctgtctgga tttttgatat ctctgtatta
    19681 tctgcataaa caatctttga cgtttctgta atgtttccta gttctgtctg cagtgctttt
    19741 ttagtcggca attgtgtgtc ttcgacgaga taggcaaaat agccagcgcc tattccaata
    19801 cctagagctg cgaacatcaa aagaagaaca gtaataaaaa cagtcaatga ttgaaaaact
    19861 ctaaaaataa tcttaggaac aaaccatttt tctttttttc ttctattctg tttttttcga
    19921 gtatttttgc gtggcaaaag ggtcacctca actaatggtt ttatttcgct aattatagca
    19981 aataatagaa caaaaaaaca gacaaggctg tttttggcag gaatttcttt tttaaaagag
    20041 ttaaaacgtg agaaaatgat ctttattata aaagagttct taagcttttc tttagagaaa
    20101 atttcagtta ctatggagaa cagggcaaag aaaaatcaat cattttcttt gtatatcatc
    20161 ttaccaatat ataggaattg ttagtaacaa ttgcctttca aggaggaatc catccatgtt
    20221 attaggctca cacgtcagca tgagcggaaa gaaaatgctg ttaggagcag cagaagaagc
    20281 agccagttat ggatcttcga cttttatgat ctatacaggc gcccctcaaa acactagaag
    20341 aaaagccatc gaagagatga atattgatgc cggaaaagcg tacatggcag aaaaaggtct
    20401 aacaaatatc gtcgttcatg ccccttatat cattaatttg gggaatacga tcaaaccaga
    20461 aatgtttcct tttgccgtcc aatttctgag agaagagatt gttcgtgcgg aagctttagg
    20521 cgctactcaa attacacttc acccaggtgc tcatgtaggg gcaggaacag aagccggaat
    20581 tgctagaatt attgaaggct tgaatgaagt tttgacaaaa gaacagacgg cacaaatcgc
    20641 tttggagaca atggcaggta aaggtacgga aatcggtcga acatttgagg aattagcgca
    20701 aatcatcgat ggagtaaaat tgaatgaaaa attatccgtt acttttgaca cctgtcatac
    20761 gaatgatgcc ggttataata ttaaagaaga cttttcaggt gtaatggaag agtttgataa
    20821 gatcatcggg ttagatcggc ttaaagtact ccatatcaat gattctaaaa atccgcaagg
    20881 ttcgcataaa gaccggcacg ccaatattgg ttttggaaca atcggattcg acacgctgaa
    20941 taagatcgtc catgatccaa tgtttgacaa tatttcgaaa atcttggaaa ctccctatgt
    21001 aggcaaagat aaaaagaaag cttacgcacc atatggaaaa gaaatcgcca tgttcaaagc
    21061 gcaaacattc gatccagaag tgtttcctga gttattagca gaagaagtga tactctaaga
    21121 ttttctatct gacagcagca aaaaaggacc aggcacattg ccttgtcctt ttttgtttaa
    21181 gaaacaaagg agtacttttt gcagtctttt tcatcatgca tcttctcccc cctcttttta
    21241 aatttagaca aacaaggtat actggtagaa aagtaaaaaa acgaaaagtt tcacaagcaa
    21301 gtggcaaaac gtgattggaa ggacgaaaag atgctgaaca ctttaaaacc cttgatcaac
    21361 caactaaaaa agaaacatac atactttatt cttggcatag ccctattatt tacaattatt
    21421 cgtttgtatt tatatagtaa agcaatctat ggaacagaca tgtcggcaac ctatgatgac
    21481 cagctgctga ttcgctatgc tcaaaatatt ttggacggta aatggctagg ggagtatacg
    21541 actacaacac tttccaaagg aatctcctac agcttatttt tagtacttgc acataaactg
    21601 catctttctt atgcagtttt gttcggcatg ttcagtattt ttgccagtat attggttgca
    21661 ctcgcgttta aaccgattgt gaagaacaag tatcttcttt taagtatcta tttttatttt
    21721 ttgttttctc cgattagttt tacacatgaa tattccactc gaacatatcg taatgccctc
    21781 gtgattccgg ctgttgagat cattatcgct tgtttattag ctatttatta ccgcaaatac
    21841 ggttctagca gaaaattgct gccatggttt ttagggcttt ctgtcgcttt tcctttcttt
    21901 tggttcattc gcgaagattc tatctggctt cttcctttta ctgtagtagc tttgggtatc
    21961 agtttcgtcc agatcttgct agggcaagac ttttctttca aagaagtaaa gaagttaaaa
    22021 aaacgactct ttcttctctc gaaacgggaa tggctgaaac tttcattgtt tttacttcct
    22081 atagttggct tttccatcgt ctatcaatgg atcgaatata aaaatgagac aacatacggt
    22141 attgcaacca tcaatgaccg ttccagtgga gaatttggac gtttgatgaa acaactgatc
    22201 cgaatagatg atgaaacgaa cttgaataaa gaaaacagtg agatatgggt ctcacatgct
    22261 gcactaaata aagcattagc cgcatctcct acattccata ctgtttctac ccgtattgac
    22321 gaattatata cgactcacgc gtggactcaa ggtggaaaag taaaagaatt acccggagat
    22381 atcatctttt gggcattgcg agatgtggta agcgaatctg gttattacca aaacaatgca
    22441 aaagaaacca atgatttttg gaaaaaagtc aaccaagaat tagaagaagc ctatcacaag
    22501 ggaacaatca aaaagaaaaa agagttctat ctgatgggat caggagatgg aaagacgatg
    22561 agtgatctgc caatcgtatt cgatttcctt caatcaggat acgcttatgc atggcgatat
    22621 aaagatttcc atcaaggagg aaattatagt ttcggaacgc aagaacaagt caaacaggct
    22681 caagaattat taaaccttcc gctaatggag aattggatcg atagtaatga accgagaaca
    22741 cctaatttcc aattaacaaa atcagcaaaa gtcgcgaatg tcatcataaa aatttaccaa
    22801 gttctggcta ttcccgtgct gattgcttca atagcaggga tattacttat gatactagga
    22861 accgtatttg caaaagctga tcgtgcctat tatagttcct ctttgattgt ggtcaccgga
    22921 ttaatactga cacaattggt ttttcttttc ggtgtctcat ggttttgttc ttatgcgcca
    22981 gaaaaaaagg actatttttt aagtgtctac actggtgcag gcgtgccatt aacgcaattc
    23041 atcgtagtaa caagcatgct agttttcagt aaattaaatc tgaaaaaagc tttatcttct
    23101 aaataaaaag acccttggta gctagatttt taacctaact atcaagggtc tttgtgtgga
    23161 aaaatcagtt tttattcgat ttcttctacc gtttcctgca aaagctgttc atattttttg
    23221 tttcctttat ataaatcacc aatcgtccat gccaacagga gaaggacaag aacaatgatt
    23281 acataagcga tgtgatcagc caatgctagt tgagcggaag tggcttgctc gccgaagaaa
    23341 ctttcgatct gtcctggtaa tcctcgtaag ttcaagtaag ttagactaat cacagagaac
    23401 cagccaagaa gttttacgat gaatgaattt ttaaatcggc tacccatctc cgcttggctg
    23461 ttggtcatca tcagtaaagg aatcatcgaa aacggcaacg caaaagccaa gaacacctgg
    23521 gattcattca ttaagttatt caaagcggca tgttcttcga tcgttccttt tgtacttgta
    23581 aacaaaacac aaatcaatac aggaatcaca gaaagcaatc gtgtgaccag tcttcttagc
    23641 caaatcggga tacgcatatg gatgaatcct tccatgatga cttgaccggt caacgtacca
    23701 gtaatagtag agttctggcc agaagcaaga agtgctacag caaataaagt ggacaagata
    23761 ccagattttg ctacagcaat caaaatacca ttgctcatag ctgatggatc agataaagct
    23821 tcatacagac caaaaaagga aggatcttgg acagaaccgg ttttgaagac agcgacaccc
    23881 atgatcaata gcagtgcatt gactagaaaa gccattgaca actggatatt agaatcccaa
    23941 gtagaaaaac gtacagcttg tgctacatct tcttcgtttt ggtgatcgat cttccgagtt
    24001 tgtgaaactg ctgaatgaag atataagtta tgcggcatga ctgttgcacc aataatccct
    24061 aaagctccag tcagcggagt ttcgccgccg atgaccggtg agtctgagaa tgtatgactg
    24121 ttagggataa agccagcaaa taaactgtgc caatctgggt tagacaagcc aacttgatag
    24181 acaaaaacga ataaaatgac gaaaattaag cagacaacca gtgcttcgat cttacgaaac
    24241 ccgacttttg tcagtagtaa gagaacgaaa acatctaata cagtaataaa gacggcaatc
    24301 accaatggaa tatcaaaaag taagtagagg gcgattgctg caccaatgac ttctgcaata
    24361 tctgttgcca taatggctaa ttcagtcaag atccataaaa tgacacctaa tgtcttactt
    24421 gtgcgtgctc ggattgcctg tgccaaatcc atttggctca cgattcctaa ttttgctgcc
    24481 atatactgta aaagcatagc aatcagacta gacatcaaaa taactgacat caataaatat
    24541 tggaaatttt gtcctccggt gatcgaagta gaccagttac ccgggtccat atacccaact
    24601 gctactaacg caccaggtcc agagtaagca agaagtgttt taaaaaaact tttccccttt
    24661 ggtacatcaa ctgttccatt tacttcagct aaagataaac catttgtatg ttcaataagt
    24721 cgatgcttat gaaattgttt attggattga ttattcaaga aaaaaccacc tttcctattt
    24781 atctacgatt acattctaca cctcaaaaag aaaaaattaa agaaaaagat tcggtacgcc
    24841 taattttttt tcatggcgcg tcttgctaga tgacgaaaga aattttgttc tcctctaaaa
    24901 aaaccactat tttttgcatg aaactaaaga atacgccttt cttttttcaa taaactttag
    24961 taaaataagt cattgagagc aattcggaaa tagaaaggag agatttatat gttatcaaca
    25021 ggtattgtcg tacttattgc tattattgct ttgctaatcg gcgcagtagg tggctttttc
    25081 ctagcgcgta aatatatgaa agactattta gaaaaaaatc ctcccgttaa tgaggacatg
    25141 ttgcgatcaa tgatgatgtc aatggggcaa aaaccatctg agaagaaaat tcgccagatg
    25201 atgcagcaaa tgaagaacca agggaaaaaa taggttctga cccaacgact ttttacgggc
    25261 tgattttttc agtccgtttt ttatttcgta atttgttaga ctattcagat tattcagcaa
    25321 aaaagacaag cacagacaat cctaggctct tgataaagac aaaggaggag taaaatgtcg
    25381 atttttagaa aattaggctg gttttttcgt caggaaaaga aacattattt aattggggtt
    25441 ttctcgctat tcgcagtggc cttggtacag ttagttcctc caaaagtgat tgggatcatc
    25501 attgatgaga tcgccgataa aaatttatca ataaagatca ttttcgggtg gatcttccta
    25561 ttgattatcg ctgctttttt acaatatctt tttcgttata tctggcggac gaatatttgg
    25621 ggaagtgcag ctcgattgga gaaagaactt cggcagcagt tattcaaaca ttttacagaa
    25681 atggacaatc tcttttacca aaaatatcgg actggggatt tgatggctca cgcaacgaat
    25741 gatttgaatg cgatccagaa tgtagcagga gctggtatct tgacctttgc agattcagtg
    25801 attacgggcg gaacaacgat cattgcgatg attttgttta ttgattggcg cttaacactg
    25861 attgcgctga ttcctctgcc tttgctggcc gtcacttcac gagtgctggg gtcaaaactc
    25921 catgatgctt ttcgtgattc gcaagaagct ttttctagta tcaatgataa gacacaagaa
    25981 agtatcactg ggatcaaagt cattaaaaca tttggacaag aaaaagaaga tctagcggat
    26041 tttgaagaaa agattgaaga tgcaattatt aaaaacaaac gagtcaattt tttagactcc
    26101 ttatttgatc cattcattac cttgattatc ggtctgtctt atgttgcaac aattatttta
    26161 ggtggaaagt atgtcttgga aggcacgatc tcgatcgggc agatggtctc atttattagt
    26221 tatatcggta tgcttgtttg gccgatgttt gcgattggtc gattgttcaa cgtattggaa
    26281 agaggaaatg cttcttatga tcgcgtcaat gaacttttgc atgaaaagac gcatatcgtg
    26341 gaacaactgg gtgcagtgca gaccccggca aaaggcgagc tgaatgtagc tatttcctct
    26401 tttaattatc cgaaagaaga atcaccagca ttagaacaaa tcaagtttgc gatccaagaa
    26461 ggagaaacat taggtattgt tgggaaaact ggtgcgggaa aaacgacgat catcaaatta
    26521 ctgatgcgtg aatacgatga ttatcaaggg aaaatccaat ttggcggacg ttcgatcaaa
    26581 gaatattcat tgaatgcttt attaggatca atcggctatg tgcctcagga tcacttcttg
    26641 ttttcgatga ccattcgaga taatatccgt ttttctaatc caaaactttc agaagaagaa
    26701 gtagagcatg cggcttcttt agcatttatc aacaatgaaa tcaaggaatt accaaaaggt
    26761 tatgatacct tagttggaga acgtggagtc tctttatctg gcggacagaa acaaaggatt
    26821 tcgattgctc gagcattgat caaaaatcct gaattattaa tattagatga tgcactctct
    26881 gcagtagatg ccaaaacaga agaagctatc ttgcataatt taaaagaaat aagaaaagag
    26941 aaaacaacta tcattgcagc tcaccgttta agtagcgtga tgcatgcaaa agaaatcatt
    27001 gttgtcgatc aagggaaaat cattgaacga ggaacccatc atgagctgct tgcactaggc
    27061 ggctggtatg aacaaatgtg ggagaaacag caattagaag cgaagataga aggaggcgag
    27121 gcctgatgga tgaaaattat caatcggaat ggacaaaatc gattccactt aaagaacaga
    27181 aaaagatcat caaacgactg atgaagttcg ccaaaccttt tagaagaacg ttcatcgttg
    27241 ctatcttatt tgcgtttgct ttgtctgtga tcaatgtcct attgccaagg atcatccaaa
    27301 cttttatgga tgatcatctg gctaaacagt cggctacgac acaagttatt ttgttttttg
    27361 ctggtatgta cttatttgga gtcatagtaa aaagtatcat ttggtttttc caatggtatt
    27421 tgtattccat ggcctcattg aagacctatc agtatattag ggtaaaatta tttgaaaagc
    27481 tgcatacact aggtatgcgt tattttgatc agacaccggc aggatcaacc gtttctcgag
    27541 tgacgaatga cacagaaacg ctatttgaat tttggtacgt ctttttgatg gtattgactg
    27601 ggatctttgc agtcgtttct tcattccttg ccatgtttca aatcaatgga gaaattgcct
    27661 tgtggacttt ggtgtttttg ccgattttag gaatcgtcat ctggtattac cagaaattca
    27721 gttctcgtat ttaccgaaat atgcgggaaa aattaagtca gctgaacacg aaattgaatg
    27781 aatcgatttc cggcatgcgt attatccagc agtttcgcca agaagctcgt ttagctaaag
    27841 aatttgaaca agtgaacaat gagtatttag acacgcgtta tgcgatgatc aaaacgaatt
    27901 cgattctgtt aagtccgatc atcaattttc tatatgcatt ggcgattgcg ttggtgttga
    27961 ctttgtttgg gatcgatgcg ttgcattctc ccgtagaagt tggactgatc tatgcattta
    28021 cgacctatgt ccagggattt tttaatccaa tgtctcaaat gatggacttt ttgagcatct
    28081 tcacagatgg gatggttgct ggtagtcgta tcttgaagat tatggatacg gaagaatatg
    28141 caccacaaca aaaccctgga gcagatcaaa tgattacttc aggtaagatt gagtttcgca
    28201 atgtaagctt ttcttatgat ggagagcaca atgtattaaa caatatctct tttgttgcta
    28261 atccgggaga gaccgttgca ttagtgggac acaccggaag cggaaaaagc tcgatcatca
    28321 acgtattgat gcggttttac gaattttatg aaggtcagat attgattgat gatcatgaca
    28381 ttcgagaata tccaatggaa gagttacgta agaaaatggg gctggtcttg caagatgctt
    28441 tcttgtttta tggggatatc gcaggaaata ttcggatgat gaatccagct atcacagacg
    28501 aacagatacg ccatgcggct gagtttgttc aagcaaacaa attcatcgat gaattaccaa
    28561 aaggatatca ctcaaaagtg atcgaacatg gtgccagcta ttctagtgga caacgacagc
    28621 tgatcacttt tgcgcgaaca atcgtgactg atcctaaaat tctagtattg gatgaagcaa
    28681 cagcaaatat cgatactgaa acagaaggat tgatccaaga aggattggca aaaatgcgtc
    28741 aaggacggac aacgattgcg attgcccatc ggctgtcaac gattcgagat gcagaattga
    28801 ttttagtttt agacaaaggt catatagtag aacgcgggaa tcatgaaaca cttctagcgc
    28861 aaaatggttt gtatgcagat atgtacaaac tgcaaagcag tggtatataa aagatgttgt
    28921 gacagtagaa ttttgaccta agcactagag tgaaaataaa acatagcttc tcatattttt
    28981 catctgtgag agttagtgtc gtagtcacca gatttaaaaa aagattcgac agctagtcag
    29041 aagtgcctaa tagccactaa gaaagcgttg acaaaagcat ttgctttcga taatataaag
    29101 aaaacgatac aacttggata gtgattgcct gatgcaagct aaacctgatg agtctcccat
    29161 ggtttcatgg acggcttttt gggtttttct ttttgttttg gatacagccc tatacatcat
    29221 ggaaaggagc aaaacatgct gattgagaaa gtactgaata acaacgtcgt aataaccagg
    29281 gatgaaagaa atcaggaaat gatcgtgatg ggaaaaggtt tggcattcaa aaagaaagtg
    29341 ggtgacccga tttttgactc atgcatcgac aaaaaatttc agttgacaaa tgacgggctt
    29401 tcacaacagt tccaagaatt ggcacaagcg attcctcttg agtacttaga aatttcttgt
    29461 gaaatcattg aatatacaaa agaggctcta caagtccagt tgaatgattc tgtgtatatt
    29521 gcattgacgg atcatttgta tacggctatc gaacgtcaca aaatgggaat atttgtacca
    29581 aatgtattgc tgtatgatat caaacaacta tttcctaaag agtatgccat cgggaaaaaa
    29641 acagtgaaga aaatctcgga taaataccac atcaagttat cagaagatga agccggattt
    29701 atcgcattgc atttagtcaa tgcacaaggc attcaaagcc agcaagacaa tacctacctg
    29761 tttactataa caatacagga tattctaaat atcgtccgct attactataa gattacattt
    29821 gatgaagatt cagtccattt ttttcgattc gtcacacact tacgtttttt tctgacacgc
    29881 gtcctagatg gagaaaaatc agaagtggga gaagtttctg aggaattgtt tcaggttatt
    29941 caaaaaaaat ataaggatac gtttggttgc gttgagaaaa tagcagactt tttagccgag
    30001 acgttcaact accagatgtc cacggacgaa aaaatgtatt taacgatcca tactgcgcgt
    30061 ttagtgcatg agaatcgttg taaataaaaa aaggaggata aattaacaag aacgtatgaa
    30121 ataagggtag tgacattcag ttggctaaac cttccactaa taaattggag ggaatagaaa
    30181 tgggaaaata ccaagaatta gcgagaaaaa tcgtagaaaa tgtcggcggg aaggaaaata
    30241 tcaatggttt gacacattgt atcacaaggc ttcgtttcaa attaaaaaac gaagaaaaag
    30301 caaatgatga gatcttgaaa aatatggacg gtattgtaac ggtaatgcga gcgggagggc
    30361 agtatcaagt tgtcattggg aatcatgtgc cagtcgtgtt tgaagaggtt ataaaagcag
    30421 gaaaccttac ttttgatgag gctgtttcaa cagaaaaaat gagaccgttt gatatgctta
    30481 ttgatggaat atcgggctgt ttccagccat tccttgctat cctagctgct ggcgggatga
    30541 ttcgtggact gacagcattc cttgtcttct taggcgcgtt tgaccgcggt tctggcacat
    30601 ttgttatgtt tgataatatc ggtgattctg tattccaatt tatgcctgtt attatcggtt
    30661 taacagctgc ccgtaaattt aaagtaaatg aattcgtagg gatgttgctt ggcgcagcat
    30721 taatgaatcc tagcttgtcg ctggaagctt tgagtggtgc agcagaagca ccactaacga
    30781 ccatattttc tggcactatt tttgaagcac ctatttatca aacagtattc ggcattccct
    30841 ggattgcgcg taactacgct tcttctgtca ttcccattat ctttattgtg ttgttagcta
    30901 gccaaattca gaaaccaatt aaaaagttag tacctgagat gatcgcgaat ttcttcgttc
    30961 cattcttcac cgttcttatt acaatgcctt taggtttttt actcgttgga ccagtattca
    31021 cattcgcaac tgatatttta atggctggat ttgaaacact tttagcactt tcgccagtta
    31081 tttatggagc aatcgtaggc ttcttctggc aaattctggt gatgtttggt ctccattggg
    31141 caatcgtacc aatgggattg atgcaatttt ctgtgaatgg ttggcaaaat attatgacac
    31201 ctgttgcagt agtgtctttt ggacaaactg cagcacttac cgcactctac tttaagctta
    31261 gaaatccaaa agacaaagca attgcaatac cagccattgt ttctggtatt gtggggatta
    31321 cagagccagc tatttatggg tttacattac cacgtaaaaa aatatttatt tttacctgtg
    31381 tcggtggtgc gttagggggc gcgtatagtg ggttaatgaa tttaacttct tggaatcaag
    31441 gtggactagg catctttaca attcccaact atattagacc agatggtgat ttaacagatg
    31501 tgattaatgt attgattggg atcgccattg cgatggtggt atcctttacg ctgacttttt
    31561 tcttttggaa ggatgaagca ggtgaaacag atgatataca gaaaaaatca ggtaaagaaa
    31621 ttgtaaaaac accgatacaa ggacaaatcg ctccactaaa tgcagcaaag gatgcagctt
    31681 ttgcacaagg aacactaggt cgaggaatct tgatctaccc tgaaaaagga gaagtacgtg
    31741 ccccttttga cggaacagtc atgaccctgt tcccgacgaa acaggcaatt ggaatggttt
    31801 ctgaaacagg gctggaattg ctgattcatg tcggattgga tactgtgcag ttggaaggga
    31861 aatattttga aagcttagtg cagcaaggat aaagagtgaa acaaggcgat ctgttgctac
    31921 gcttcgacaa aaagaaaatc gaaaaggaag gatacagttt agaaacacct gtgatcgtca
    31981 ccaattacac ggattatttg gatattttgg aaaaccaagg ggagacggtc gggccagacg
    32041 atgcacttat caccgcactg acctgaacgg ttaggtgaag aatggactag gcttaactat
    32101 tctttttatt ccacttcttt attttcaaaa ttgaaaggct ctttcatgat ggctgagctg
    32161 atagggcgtt tatcgaattt ttgaccgaca gaaattagat ggactaaatt ctgttgataa
    32221 ttttctgcaa aaacacaact atataacacg tctagaagat aacaaattga tgtattgatc
    32281 gtaaaattac caatttttga atacagtcgc tcgcgtgtcg taagtcgcaa agcggctgta
    32341 ctcattgcag atagcgttga atcaccgata ctggtcaaag ctaaaattgg aacatgattt
    32401 tgtttcaata tttctgcggt acgaaggatc acattgtttt cacccgtata agaaatcaga
    32461 atcccgcaca tatcactcgt gcaattgtac gcttcataga atgattcacc aaaagtggtg
    32521 acaatcgtga catccatttt gatgcgcttc attttcagca tgaaatcttg agaaatcaaa
    32581 gtattcgagt tactggcgaa aatcacgatt ttttttgctt gaagcattag ttgtttggct
    32641 ttttgcagtt ggtcatgatg aatgagagaa agtgtatcat gaatcgtcat ctgttctaaa
    32701 gaagccattt tatttgcgat cgtcataata ccgtctgtgg tggaaaaagg aagattcgca
    32761 tctacgtctt gaaaattccc ttcaagatag gctgtttcag ctaaaaaagc attacgaaaa
    32821 ctgacccagc catcaaaacc catttttttt gctacacgga tgagtgtaga ggggtggaca
    32881 taacatcgct cagcaatcgc ttggatggtc atttcgccaa gtgctggaga atcgcttaag
    32941 agaaactgga tcacttcatt ttctgaagga gaaaaggaga tttctttgat tttttcacgt
    33001 aataacatgt agataccttc ttcttatttt tttacgtaaa ttataacttt tttg
//
LOCUS       DAYYCY010000012        32849 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000012 DAYYCY010000000
VERSION     DAYYCY010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32849
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.012"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..364
                     /locus_tag="ACHAZB_000461"
     CDS             <1..364
                     /locus_tag="ACHAZB_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6037 family protein"
                     /protein_id="HFM8613167.1"
                     /translation="SNLGDVLFQFSQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSE
                     DPRKKYCFSVRRNPLKGNGELGKRSPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPD
                     DEETDEGIIAKWTKNKIE"
     gene            436..831
                     /locus_tag="ACHAZB_000462"
     CDS             436..831
                     /locus_tag="ACHAZB_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM8613168.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            815..3268
                     /locus_tag="ACHAZB_000463"
     CDS             815..3268
                     /locus_tag="ACHAZB_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8613169.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            3265..5292
                     /locus_tag="ACHAZB_000464"
     CDS             3265..5292
                     /locus_tag="ACHAZB_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFM8613170.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            5289..6311
                     /locus_tag="ACHAZB_000465"
     CDS             5289..6311
                     /locus_tag="ACHAZB_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HFM8613171.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            6328..7257
                     /locus_tag="ACHAZB_000466"
     CDS             6328..7257
                     /locus_tag="ACHAZB_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM8613172.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            7571..7618
                     /locus_tag="ACHAZB_000467"
                     /pseudo
     CDS             7571..7618
                     /locus_tag="ACHAZB_000467"
                     /inference="COORDINATES: protein motif:HMM:NF011335.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7634..8434
                     /locus_tag="ACHAZB_000468"
                     /pseudo
     CDS             7634..8434
                     /locus_tag="ACHAZB_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
     gene            8672..8908
                     /locus_tag="ACHAZB_000469"
     CDS             8672..8908
                     /locus_tag="ACHAZB_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8613173.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            8915..10867
                     /locus_tag="ACHAZB_000470"
     CDS             8915..10867
                     /locus_tag="ACHAZB_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFM8613174.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(10939..11436)
                     /gene="dfrG"
                     /locus_tag="ACHAZB_000471"
     CDS             complement(10939..11436)
                     /gene="dfrG"
                     /locus_tag="ACHAZB_000471"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFM8613175.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            11711..12838
                     /locus_tag="ACHAZB_000472"
                     /pseudo
     CDS             11711..12838
                     /locus_tag="ACHAZB_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetM/TetW/TetO/TetS family tetracycline
                     resistance ribosomal protection protein"
     gene            12936..13124
                     /locus_tag="ACHAZB_000473"
     CDS             12936..13124
                     /locus_tag="ACHAZB_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HFM8613176.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(13182..13535)
                     /locus_tag="ACHAZB_000474"
     CDS             complement(13182..13535)
                     /locus_tag="ACHAZB_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8613177.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            14066..14488
                     /locus_tag="ACHAZB_000475"
     CDS             14066..14488
                     /locus_tag="ACHAZB_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFM8613178.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            14485..14715
                     /locus_tag="ACHAZB_000476"
     CDS             14485..14715
                     /locus_tag="ACHAZB_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8613179.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            15213..15413
                     /locus_tag="ACHAZB_000477"
     CDS             15213..15413
                     /locus_tag="ACHAZB_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HFM8613180.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            15440..16633
                     /locus_tag="ACHAZB_000478"
     CDS             15440..16633
                     /locus_tag="ACHAZB_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8613181.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(16701..18266)
                     /gene="guaA"
                     /locus_tag="ACHAZB_000479"
     CDS             complement(16701..18266)
                     /gene="guaA"
                     /locus_tag="ACHAZB_000479"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFM8613182.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(18325..19248)
                     /gene="coaA"
                     /locus_tag="ACHAZB_000480"
     CDS             complement(18325..19248)
                     /gene="coaA"
                     /locus_tag="ACHAZB_000480"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFM8613183.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            19444..20049
                     /locus_tag="ACHAZB_000481"
     CDS             19444..20049
                     /locus_tag="ACHAZB_000481"
                     /EC_number="2.1.1.172"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0052916 - 23S rRNA
                     (guanine(1835)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8613184.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            20357..21373
                     /locus_tag="ACHAZB_000482"
     CDS             20357..21373
                     /locus_tag="ACHAZB_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8613185.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            21514..22815
                     /locus_tag="ACHAZB_000483"
     CDS             21514..22815
                     /locus_tag="ACHAZB_000483"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HFM8613186.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            22861..23523
                     /gene="deoC"
                     /locus_tag="ACHAZB_000484"
     CDS             22861..23523
                     /gene="deoC"
                     /locus_tag="ACHAZB_000484"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFM8613187.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            23544..23942
                     /locus_tag="ACHAZB_000485"
     CDS             23544..23942
                     /locus_tag="ACHAZB_000485"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFM8613188.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            24008..25090
                     /locus_tag="ACHAZB_000486"
     CDS             24008..25090
                     /locus_tag="ACHAZB_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8613189.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            25302..26864
                     /locus_tag="ACHAZB_000487"
     CDS             25302..26864
                     /locus_tag="ACHAZB_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8613190.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            26857..27990
                     /locus_tag="ACHAZB_000488"
     CDS             26857..27990
                     /locus_tag="ACHAZB_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8613191.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            27990..28946
                     /locus_tag="ACHAZB_000489"
     CDS             27990..28946
                     /locus_tag="ACHAZB_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8613192.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            29045..30211
                     /gene="deoB"
                     /locus_tag="ACHAZB_000490"
     CDS             29045..30211
                     /gene="deoB"
                     /locus_tag="ACHAZB_000490"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFM8613193.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            30235..31053
                     /locus_tag="ACHAZB_000491"
     CDS             30235..31053
                     /locus_tag="ACHAZB_000491"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFM8613194.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            31070..31777
                     /gene="deoD"
                     /locus_tag="ACHAZB_000492"
     CDS             31070..31777
                     /gene="deoD"
                     /locus_tag="ACHAZB_000492"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFM8613195.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(32075..32761)
                     /gene="gpmA"
                     /locus_tag="ACHAZB_000493"
     CDS             complement(32075..32761)
                     /gene="gpmA"
                     /locus_tag="ACHAZB_000493"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFM8613196.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
BASE COUNT        10713 a         5784 c         6777 g         9575 t
ORIGIN      
        1 tagtaacttg ggagatgttt tatttcaatt tagccaaact cttgctcgat ttattccgac
       61 agaagttagt gagaagaaaa atgaagacca aaaagaagcc atgtgtttct cattaagtca
      121 aagtgattca gaagatccca gaaaaaaata ttgtttttca gttagaagaa atcctttaaa
      181 gggaaatggt gagctgggaa aacgaagtcc ttttaatgac aataaaacga gattgtatag
      241 acccagctta tatgaacgat tagggtcaga taccaattta agtttcagat attcaatgaa
      301 tccagatgat gaagaaactg atgaaggtat tattgcaaaa tggacaaaaa ataaaattga
      361 atagttaaag cattgaaaga gcaatcacct attttgattg ttctttttct atttttacgg
      421 aaaggacgtg aagacatgaa aaaaataaga agttacacca gtatttggtc agtagaaaaa
      481 gtgatttatg cgattaatga tttacaactc cctttcccaa ttacctttac gcaaatgaca
      541 tggtttatcg tttctttgtt tgtcgtcata ctctttggca gtttaccacc actttctctg
      601 attgacggag cactactcaa atacttaggg attcccattg ttctcactgg gttcatgtgc
      661 caaaagacct ttgacggaaa gaaaccctat ggatttctaa agtctatcat tacctatgct
      721 ctacgcccta aagtaaccta cgctggcaaa caaatacgat taaaaaaaga aacaatgaat
      781 gaatcgatta cgattgtaag gagtgaacaa ctagatggca tatccgatta aatatatgga
      841 agataatctc gtatttaatc atgatggcga agtattcgct tattatgaat taattcccta
      901 taattacagt tttttaagtt ccgatgaaaa aattcaagtt catgataatt tctgtcaatt
      961 gattgcccaa aatcgtgatg gcaagattca tgctctgcaa ctagcaaccg aatcaagtat
     1021 tcgagccaca caggaacaat cgaaaaaaga aatcactgga cgattaaaag agatagctta
     1081 cgaaaaaata gatgaacaga ccgaagcgtt aatttctatg attggtgaca atcaagtaga
     1141 ctatcgcttt tttattggtt ttaaactcta cctcaatgaa caggaagtat ccgtaaaaaa
     1201 tgttgggaaa gaaattcaaa aagcatttgc ggaattcttt catggtgtga atcacaaatt
     1261 aatgggcgat tttgtttcta tgcctatggc ggaaatcgaa cgcttttcta agatggaaca
     1321 gttgttagaa agtaaattat ccagacgctt tcagattcgt aaattggaga aagatgattt
     1381 tggttatatt attgaacatc tttacgggca aacaggtgtc ccttttgatg actacgacta
     1441 ccatttaccg aaacgatacg aagataaaaa agtgttggta aagaaatacg atattctcaa
     1501 acccactcgc tgtttaatcg aagaaaatca acgctattta cggattgaac aggaagatac
     1561 cactacttat gtggcgtatt ttacaattaa tagtattgtt ggtgaattgg atttccctaa
     1621 tagtgaaatt ttttattacc agcaacagca gtttactttc cccgttgata ccagtatgaa
     1681 tgttgaaatt gtcgccaata aaaaagcctt atctaccgtt cgcaataaaa agaaagaact
     1741 gaaagattta gatagccacg catgggaaag cgacaacgaa accagtacta atgtggtgga
     1801 cgctttagaa tctgtcgatg aattagaaag taatttagac caaaccaaag aatctatgta
     1861 caaactaagc tatgtggtac gggtatcagc aaagaatttg gacgagctga aacaacgttg
     1921 tgatgaagta aaagattttt atgatgatct gtctatcaaa ttagtacgac cttttggcga
     1981 tatgctaggc ttgcataatg aatttattcc ctcaagcaag cgatatatca atgactatat
     2041 tcaatatgtc acgtcggatt ttttggctgg tcttggtttc ggtgctacac aaatgattgg
     2101 tgaaaattct ggtatttacg tcggttacaa tctcgataca ggcagaaata tttatctcca
     2161 acctagcctt gcaagtcaag gaattaaagg ctctgtaaca aatgcattag cgtccgcttt
     2221 tattggttca ttaggtggcg gaaaatcatt ctctaacaat atgctggtct attattcagt
     2281 actctttggc ggacaggcaa ccattgttga cccaaaagct gaacgtggtg aatggaaaga
     2341 aaccttacct gatattgccc atgaaatcaa tattgttaat ctcacgagtg agaacgacaa
     2401 taaagggtta cttgaccctt atgtgatttt aaaactcccg aaagattcag aaagccttgc
     2461 cattgatatt ctcacgttct taacaggtat ttccagccga gatagcgaga agtttcctgt
     2521 cctgcgtaaa gctattcgga gtgtaactca aagcaaacaa cgagggttgt tattggtgat
     2581 tgatgaattg cgaaaagaag atacgcccat cagcaatagt attgctgacc atatcgaaag
     2641 ctttgtcgat tatgattttg cccatctgtt attcagtgat ggcagtgtca cccaatccat
     2701 tagcttggaa aaacaactca atattattca agtggcagat ttggtcttac ccgattccaa
     2761 tagctctttt gaagaataca ctaccatgga attattaagt gtggcaatgc tgattgtcat
     2821 tagtaccttt gctttagact ttatccactc tgaccgttcc atttttaaaa ttgttgacct
     2881 tgatgaggct tggagttttt tacaagtggc acaaggaaaa gctctatcta tgcgattggt
     2941 tcgagcagga cgtgccatga atgcaggggt ctatttcgtt actcaaaacg cggacgattt
     3001 actagatgaa aagatgaaga ataatattgg cttgaaattt gcctttcgtt ccactgacct
     3061 agtggaaatc aaaaagacct tagaattttt tggtgtggac aaggaagatg aaaacaatca
     3121 gaaacgctta cgtgaattag aaaatggaca atgtttagtt tcagacatct atggtcgtgt
     3181 aggggttatg cagtttcacc cgatttttga agagttgctt catgcctttg ataccagacc
     3241 gcctgtgcga aatgaggtgg aagaatgaca caaaaacaaa agaaatttct tcactatagt
     3301 tggataacgc tcctttgtgt gggcgttatt ttacttttat taggcacgct gggcaatgca
     3361 gtacaagcga caggcttagt tgatgaaacc attgatacaa gcaatgaata ctccaagtat
     3421 ggtttgaatc aatatcagct tgattattat gtggacaata gctggggctg gcttccatgg
     3481 aattggagtg atggcatagg acaatcggtg atgtatggat tgtatgccat tactaatttt
     3541 atttggacaa tttctcttta tctatcgaac gccacaggtt atttggtgaa agaagcctat
     3601 gccttagatt tcattagtca aacatcccaa gcaattggcg aaaacatgca gacgattgca
     3661 ggcattacac cgaatggctt ttccagttca gggttttatg tcggtttcct ccttcttttt
     3721 attctcattg ttggaatcta tgttgcctat acaggattgg ttaagcgaga aacgacgaaa
     3781 gtgatacgag ctgttttaaa ctttgtcgtg gtgtttattc tatcggcttc ctttattgcg
     3841 tatgcacccg attatattgc gaaaatcaat gatttttcca aagatgtcag taccgccagt
     3901 ctgaatattg ggacaaaaat tgttcttcct aacacggata gtcaagggaa agacagtgtg
     3961 gacatgatac gtgacagctt attctctgtt caagtaaagc aaccatggct attgcttcaa
     4021 tatggctcca ctgatgtaga agcgttaggg gaagaacgtg tggaaagttt attgtctacc
     4081 agtccagata caaacaatgg tgaagataga gaaaatgtag tgattgaaga aattgaagat
     4141 catgacaata cctatttaac cctaccgaag acgatttcca gattaggaac agtcttcttt
     4201 ttgtttgtat tcaatattgg tatctcgatt tttgttttcc ttctaacagg aatcatgatt
     4261 ttttcgcaag tgctatttat tattttcgct atgtttttgc caatcagttt cctattaagt
     4321 atgttgccga gttttgatgg catgggcagg catgccatta ccaagttatt taatgtgatt
     4381 atgacaaggg ctggtattac cttgattatt accgttgcct ttagtatttc cactatgctt
     4441 tattcgctgt cagcaagttc tccctttttc atgattatgt ttttacaaat tgtgaccttt
     4501 gcagggattt attttaaact gggcgattta atgagcctat tcactttaca aagcaacgac
     4561 tctcaaaaca tgggcagaca agtgtttcgc agacctcgtc aaatgatgaa tcgacaaacc
     4621 agaagatttc aacgcagtat gagtaagatt tttcgtggaa aagcagggtc aacgaagcaa
     4681 aagacaatga caacaaagca gaatcaaacc aataaagcta accatactcg tcaaaatgaa
     4741 cggactacac caaagaaaga aacagcactt tccaatgtga aaaagcaaca aggcggtatc
     4801 gggcaacgat taggtgaaaa gacagcccat gtattggaca cgaaaaatcg tctagtcaat
     4861 aaaagaaagc aggcaaaaca acagatacag gcgacaccaa cccatgtcaa atatgccctt
     4921 catcaaggaa aagagaatgt cactcataat gtaacagact ttaaacaatc tatggcagat
     4981 accaaagctc aacgcaaaca acagcgtatg aaccagcagg agcaaaaacg aaagaacatt
     5041 gctcaaaagc gtttggagct ggataaagca aaagaaaaaa gacgaaaaga aaacccatca
     5101 tctcctgttc agaataacca gcagcatgtt cgcaagcgtc agcaaaccgt cacgcctgtt
     5161 ttgtcacaaa acactaaacg tgaaaatcat cagcgaccat tgaaaaaaga gcaaaaacaa
     5221 aagacaccac cgaagcaatt caatcagagg aagctaaata aaagaacgga taagcgagga
     5281 caacaaacat gaagctaaaa ataacagtga tttgtagtgt ggtcttctct ctcttctttt
     5341 ttctcttatt attcctagtc attttctttg cagatgatac ggacagtggc gaaaacaaca
     5401 aggattcttc tattccacaa ggcggtgtga ccgtttcacc agaagtgttg gcacaccgtc
     5461 cgttgattga aaaatatggt aaggaatatg ggattgaaga ctatgtttct tatatcttag
     5521 ccattatgca agtggaatcg ggcggtacag cagaagatgt tatgcaaagt tccgaatctt
     5581 tgggtttacc gcccaacagt ttgagtaccg aagagtccat caaacaaggg gttaaatatt
     5641 tcagtgaatt attaaccagt gcagaacaac aaggagtgga tatagattct gttatccaat
     5701 cttataatta tggcggtggc tttttaaatt atgtgaggag tcatgggaaa aaatatacct
     5761 atgaattagc cgaacagttt tctaaagaga agtcgggtgg tcaaaaagca gactatccca
     5821 atcctatagc catacctgtg aatggtggtt ggcgatataa ctatggaaat cagttttatg
     5881 ttcagttggt ttcacaatac ctaaccgaca cttccccaac agaatttgat gatgaaaccg
     5941 tgcaagtcat tatggacgaa gccttgaaat acgaagggtt tccctatgtc tttggagggg
     6001 cgtcgcctac cacctctttt gattgtagtg gcttaataca atgggtgtat gacaaagctg
     6061 gcatttctct ccctcgtgtc gcccaagatc agtatgacgc tacgcaagaa atctctatgg
     6121 aagaagccca agcaggcgac cttatcttct ttcattcaac ctataatgca ggcacatatg
     6181 tgactcatgt ggcgatatat ttagagggaa atcgctttta tcatgcaggc gacccaatcg
     6241 gttatggcga cttaagcagt cgttattggc aagaccatct gattggggct agacgtgtta
     6301 ttcataacta agaaaggaat gaatcaaatg attataaaaa tcgagcgaaa agaaaagaag
     6361 gagaaaccaa agaaatctaa aaaaccgagc aagcaaagaa aagttccgat gattaaagtt
     6421 ggcacgcata agaaattaac ctttgtttta tgggttttat tgattggtag tgtcggattt
     6481 ggaatctata aaaattttac tgccattgac actcatactg tccgtgaaac agaaatcatt
     6541 aaacagcaga ttgtcgatac caaccaagta gaaagttttg tgaaatcctt tgcgaaagac
     6601 tacttttcgt ggcaacaatc ccaagaagcc attgataaac gaaatgaaaa attgacccat
     6661 tatttgacgg aagaattgca ggtattaaat gaagaaatga ttcgtaagga tattcctact
     6721 agttctagtg tgaacgatat acaggtgtgg caggtttctc aagtgaatga gaacaccttt
     6781 gaggtactgt tttctgttga acaagttatt accgaagaca aagacaagga aactatatcg
     6841 tctagctttc atgtggttgt ccatatagat gaatcagata atatggttat tatcaaaaat
     6901 ccgacaatga gtaagaaacc tcaaaaatcg gactaccaac ccaaacagct agaaagtgac
     6961 catacagtag atacagaaac gatggacgaa atcactagct ttttagaaac gttcttccag
     7021 ctctatccta ccgccacaga aaaagaattg acttactatg taagtaatca tgtgttacct
     7081 atgattaaca aggaatatgt tttcgaggaa ttggtgaatc ctatctttac cagaaaagat
     7141 aaccaagtaa tcgtgaatgt cgctgtgaaa tatttagatc aagaaacaaa ggcaacccaa
     7201 atctcgcaat ttgagttgat attagaaaaa caagataatt ggaagattgt aaaataacaa
     7261 atattggtac atgattacag atactttgta atcatgtact ctttttgata aaaaattgga
     7321 gattccttta caaatatgct cttacgtgct attatttaag tgactattta aaaggagtta
     7381 ataaatatgc ggcaaggtat tcttaaataa actgtcaatt tgatagcggg aacaaataat
     7441 tagatgtcct tttttaggag ggcttagttt tttgtaccca gtttaagaat acctttatca
     7501 tgtgattcta aagtatccag agaatatctg tatgctttgt atacctatgg ttatgcataa
     7561 aaatcccagt gataaaagta tttatcactg ggatttttat gcccttttgg gtttttgaat
     7621 ggaggaaaat cacatgaaaa ttattaatat tggagtttta gctcatgttg atgcaggaaa
     7681 aactacctta acagaaagct tattatataa cagtggagcg attacagaat taggaagcgt
     7741 ggacaaaggt acaacgagga cggataatac gcttttagaa cgtcagagag gaattacaat
     7801 tcagacagga ataacctctt ttcagtggga aaatacgaag gtgaacatca tagacacgcc
     7861 aggacatatg gatttcttag cagaagtata tcgttcatta tcagttttag atggggcaat
     7921 tctactgatt tctgcaaaag atggcgtaca agcacaaact cgtatattat ttcatgcact
     7981 taggaaaatg gggattccca caatcttttt tatcaataag attgaccaaa atggaattga
     8041 tttatcaacg gtttatcagg atattaaaga gaaactttct gccgaaattg taatcaaaca
     8101 gaaggtagaa ctgtatccta atatgtgtgt gacgaacttt accgaatctg aacaatggga
     8161 tacggtaata gagggaaacg atgacctttt agagaaatat atgtccggta aatcattaga
     8221 agcattggaa ctcgaacaag aggaaagcat aagatttcag aattgttctc tgttccctct
     8281 ttatcatgga agtgcaaaaa gtaatatagg gattgataac cttatagaag ttattactaa
     8341 taaattttat tcatcaacac atcgaggtcc gtctgaactt tgcggaaatg ttttcaaaat
     8401 tgaatataca aaaaaaagac aacgtcttgc atattaagta gttcagtttt ggagtacaaa
     8461 aagtgaaaat gtaaacctaa atgagtatca aattgcaact gaatttgata ctcgtttttt
     8521 attgttaaat caacgtttgt tgttaattta caccacaaaa tgtaagttaa aatgtatata
     8581 aaaatgcgaa cgtgaaataa ttattgtagg aatgcaaatt ggtgtgttaa aatggttaca
     8641 atttactaag caaaaagggg tgcattttcc tatgcaattt cttgagccaa aaaataagaa
     8701 cgctaagtct gtggattggg aaatatcaga gcaagtaagg gtgatagtta agcaatacgc
     8761 tgaatatgca gagcgtactg aaagcgaagc agtggatgaa ttcttattga acattttaga
     8821 cgataagaag tttattgaat ggattgccaa caagcgaagt aataagcgga ttgttgaaaa
     8881 aatgggtatc aaagatagag tgggttgata gacaatgacc aagcttaaaa gattagccac
     8941 gaaggaagat gaaattgtag atgttaaaat tccgatttca aacgaagaat taaaagatag
     9001 ggctaaacaa tatgatttat taactccaaa acgatttgca acaaggtata acaaaatgtt
     9061 gttcttgcca acgtctttta aatggaacgg ttctgaatat cccattcaat ataattactg
     9121 catcaatccg ttttgttgta attttgggaa agaacagcac aaattcaaag atgtaaaagg
     9181 gaaaccaagt cgatataaaa tgactggtag ttccaaagat aaaggtcata aaggtatgta
     9241 ctgcaatgac aatcctattg gcagaggtgt atcgcaaaac tgtacagtaa cccctttatc
     9301 taattggtct gttgttgaag aaattaaacg ccttatagaa atcaatagca tccaagatgt
     9361 tgagccagat tatcaatttc ataaagaagg ctgctcggaa gaagaatcaa ccccattcaa
     9421 tgagccgaag caattttaca aaagggggaa aagtaggggc aaatctcaac gctatcaatg
     9481 taaggcgtgt aagaagttca ctaatgttct accaaaaaga gaagaaacca ctacttatca
     9541 ccaacaaaag aacaccattc ttccaatgtt cgctaagatg gttgttggaa gggtttcagt
     9601 cagtcgtact tgtgatattt taggaattgg agttggaacc tactatcata agctggagtg
     9661 gctgtatagg cgttgtttgg agtttttaga acgatatgag acacaacccc ttcaaacaaa
     9721 gaaatttaac gagatgtggc ttaatacgga caagatgcac tactacctaa ataacgtccg
     9781 taagaaagga caaggttcaa agaaatatac ggggtttgaa gaccttaata tgcaaacgta
     9841 tatagttgta tctgctgagg tcttatcaag atatgtgttc cgttctgatg ttgcctatga
     9901 ttggaatatt tcaatggatg aattaaatga agatacaaga aaattcaaag aagaccatct
     9961 caatactttt agtcgtaaaa atgataggtt agattggtct tattatccac aagaaccatc
    10021 cgctaacgat agcgaaaata ggaatgctta tcttcacgaa ttagggaaaa ttactaatag
    10081 gagtagattt gtcgatggtt taaatgttga tgctccttat actacaactg ctcactattg
    10141 gttaattaaa caaatggtta atgccgacga atggcgaatg attagtgatg atgacttctc
    10201 tattagaaac gccttttata gagtattcac gaaagaatta aggttgagcg atgcacatca
    10261 ttttatttgc caagttaaca agacgaaaag cagaaaacaa tgcttaaaag aattcggaca
    10321 agcaaaagca gaactgctcg attggggaga tatcagagga ttcaaaacaa agtttctacg
    10381 gactttggca tctcattatc taactgaatt actaacaagt catcaattcc acgaagaggc
    10441 tattagtaaa gatggagaaa gatatcgcaa atatgccgat aaccctatca aacatccatt
    10501 agccactaag gataaaggat tttattcagt tgattgtaga acggatttat ctgctctgga
    10561 accaaacgaa atcgcaaaga tgctattaaa tgttaatgac cattcgacca atagctttat
    10621 tcaacagata agacgttata tatcatctct tgaaagacca ttaacaacag ctcgtggtga
    10681 taagaagagc tatatttacg ctaacttcaa tccgaagtat gctcaatttg cgattacaat
    10741 tctaagaact tactacaact tttgtcgccc ttttaaatca gcggataaaa aggttttgac
    10801 acctgctcaa agattaggga ttacagataa acaatttgat tggaaagata taatttattt
    10861 caaatgacaa aaagagcttg gagtaatccc aagctcttgt gctttatctt tactgttctt
    10921 cttcaattaa cgcacccgtt aactcaataa gttttttctt tcatatacat gaaaatagta
    10981 gttatacgga tttttatcat tctttatccc tttttgggca aatacctcat tccattcctc
    11041 ataattcact tctggaaaaa aagtatctcc ttcgaattca tgatgtattt ttgtgatgta
    11101 cattttctca acataaggga aaaacaaatt ataaatctgt tctcctccga aaataaaaat
    11161 ttcttcttcg tttttacata actcaaaaac atcttctatt gaatgaacaa tttcacaacc
    11221 attaaaggta aaccccttat ctctcgtcag aataatattt cttctgtcag gtaaggctct
    11281 tccgattgat tcaaggttct tcctacctaa tattatcgga tgtccctttg tagtattttt
    11341 aacatattcc cagtccttgg gaatcctcca aggaatgtca ttctctttgc caatcactct
    11401 attcttatcc atcgcagcaa tcaaagaaac tttcatttat aacacagcct ttttaactaa
    11461 tttaccatct aatttcttat ttctatcaaa aaaccttttc tcattatcct gcccgttagt
    11521 tgaataagca attttttcta taagttatat tatcataata ccacaaaatc acaaattttc
    11581 attcaattat cattttcaat tgcattttat aaatcatttt gtatgtaatt tggtatgtaa
    11641 gttaaaatga aaaaagtagt ccattttgaa gtacattttg tcattttgta ctccaaaact
    11701 gaactactta cttgcatata tacgccttta tagtggagta ctacatttac gagattcggt
    11761 tagagtatca gaaaaagaaa aaataaaagt tacagaaatg tatacttcaa taaatggtga
    11821 attatgtaag attgatagag cttattctgg agaaattgtt attttgcaaa atgagttttt
    11881 gaagttaaat agtgttcttg gagatacaaa actattgcca cagagaaaaa agattgaaaa
    11941 tccgcaccct ctactacaaa caactgttga accgagtaaa cctgaacaga gagaaatgtt
    12001 gcttgatgcc cttttggaaa tctcagatag tgatccgctt ctacgatatt acgtggattc
    12061 tacgacacat gaaattatac tttctttctt agggaaagta caaatggaag tgattagtgc
    12121 actgttgcaa gaaaagtatc atgtggagat agaactaaaa gagcctacag tcatttatat
    12181 ggagagaccg ttaaaaaatg cagaatatac cattcacatc gaagtgccgc caaatccttt
    12241 ctgggcttcc attggtttat ctgtatcacc gcttccgttg ggaagtggaa tgcagtatga
    12301 gagctcggtt tctcttggat acttaaatca atcatttcaa aatgcagtta tggaagggat
    12361 acgctatggt tgcgaacaag gattatatgg ttggaatgtg acggattgta aaatctgttt
    12421 taagtacggt ttatactata gccctgttag tactccagca gattttcgga tgcttactcc
    12481 tattgtactg gagcaagcct ttagaaaagc tggaacagaa ttgttagagc catatcttag
    12541 ttttaaagtt tatgcaccac aggaatatct ttcacgggca tataacgatg ctcccaaata
    12601 ttgtgcaaat atcgtaaata ctcaactgaa aaataatgag gtcattatta ttggagaaat
    12661 tcctgctcga tgtattcaag attatcgcaa tgatttaact ttttttacaa atgggcttag
    12721 tgtttgttta gcagagctaa aaggatatca ggttaccact ggcgaacctg tttgccagac
    12781 ccgtcgtcta aatagtcgga tagataaagt aagatatatg ttcaataaaa taacttagtg
    12841 cgttttatgt tgttatataa atatggtttc ttattaaata agatgaaata ttctttaata
    12901 tagatttgaa ttaaagtgga aaggaggaga ttgttattat aaactacaag tggatattgt
    12961 gtcctatttg tggaaataaa acaagactac gaatacgagt ggatactata cttaaaaatt
    13021 tccctttata ctgccccaaa tgtaagaacg aaactttaat taatgttcaa aaaatgaata
    13081 taataacaat caaagagcca gacgccaaga cgcagagccg ataatttgag aaatgaaact
    13141 ctcatcttat cggctctttt tgtttatctg aattttactg actagccttc aatatttctt
    13201 gcttcattga taccttttgc tagtgcttcc attaaggata gttctttgtc tgtaaagcta
    13261 tccatgtatt tttctacctg taaccttcga gtgcttttaa tcaaattatc tgtaggtaag
    13321 aaaaattcat caatagatac atggagtaat gatacaagat catacagcac ttgtgtactt
    13381 gggtgttgtc ctttgttttc tatatttgtt aaataacgtg ggtcaatttc aatcattgtt
    13441 cccacttgtt cacgagtgag accccgtttc attcgagcct ctttaatcgc taaaccaacg
    13501 gctctaaaat cgtatttatc ttctttttta cgcatactaa atcacctcta tacattttac
    13561 tgttcctata taaccataaa caggtataga aaaacatgtt atatagttta tgagttccta
    13621 tagaacactg tggaatttat taatgcgatg aatccaattt caaatagaaa ggtcgctgat
    13681 tttatttacc tatacaactt tataaaattt agtcatactc ctagaaagta gataaaattg
    13741 gcgacttaat ggagggaaaa gtcgccatga gaaagataat ctgtcatcta gttctcttaa
    13801 ttaagttctt atcaaaaaaa cttatccacc tgtactggat agatatgacc atcaatgtgg
    13861 ttttgcagat tacatagata gaaaaatcaa agcatatgaa cagagaaaac aatctgttta
    13921 tgtgcttttt tggtcactta ataaatatct tttaggattt ctcttcattt atgcaacaaa
    13981 tcccccgttc gtgtcggggt ttagtatgaa gggagataaa gaagcacatg ccactccctt
    14041 tcacgacaga aagggggtga aaagcatgaa accatcttct tttcaagaaa ccatagaaaa
    14101 tcagtttgac tatatctgca aacaagttat tgaagatgaa cgaaaagatt acttcaaaca
    14161 tctagacaga ctatcagcaa aagaagtctc gttttctgat atagataact accttgtcaa
    14221 ctctttttct actgttgata cgtacgtcac tgatttccaa ctatttaaat tatttggatt
    14281 aagaatcaat gttgaaagtg atttattaag tgaagccctg cgtcatcttt cagagaaaaa
    14341 acggaacatt attttacttc attactttat ggatatgagt gatgtggaga ttgcggagtt
    14401 actaaatttg aatcgttcga ctgtttatcg tcatcgaata agtggactag ccatgattaa
    14461 agaattcatg aaggagtgta aagaatgaaa acagcttatc caagggttcc cttccccctc
    14521 attgtaaagg cgacagatgg cgacgtggaa gcaattaatc agattgttaa acattatcga
    14581 gggtatacct caaaacgttc attacgtcgc atgacagacg aatatggaaa ttctcatatg
    14641 gttattgatg aaaccctacg tggcagaatg gaaacgaaac tcatctctaa gattttatcg
    14701 ttcgaaatta aatagtccca ttttttcgtg gaagcgtgtc gaatcgtatc cacgcttccc
    14761 gatttgggag gtttgttatt ccatcaaagc atattgtgtg ttcagtaggt tttgataggc
    14821 taacaagcca aacgttcttt gacaaccaaa taaaggcaat cgaatacgtt tcggtaagaa
    14881 aagagctgat gaactaacac gccatgacct acaaaattag tgagcgattc atgttagtaa
    14941 ttcgaaagta atctttagca ggattgctgc catgactttc ttatcgtgat aatgatactc
    15001 ccgtatagcc atagtccgag cgttagaagc gtcacaggca atgggtacgg ctacatgaga
    15061 accatgcaga agtgaaattc ctgtgagctt tgctagagct gttcgattgc taagatattc
    15121 ttctcttgtc agtgtattat tataaaacta tttgatataa ttataggtga atattggagt
    15181 tttcctagcc tataattggg aaaggagtaa taatgataac ggtcacaaaa aaggatttag
    15241 ttgagctagg atatggaact tctttttcag cagacatcat aaaaaaagca aaagaattga
    15301 tgattacaaa agggcatatc tactatcaat ctcgaaagct agatagagtc cctcgtgaag
    15361 ctgttgaaga aattctagga attcggtttt caaatgaaaa caggtctgag tagtttttgc
    15421 acagtaagga gtaaaaacta tggttaaaga cccaataaaa aaagcaaaga atggaaccta
    15481 ttattttcgt gcaaatctag gttatgattc taaagggaaa aaaattcaaa aatatcgtag
    15541 tggattcact actaaaaaag aagcaagaga agaatattcg aaactgctat tgatgaaacc
    15601 agaagaatta agtgagaata aggataaaat gacgtttaaa cattatatct ttgaaatttt
    15661 ccttccatgg tataagacac aggtaaagct cagaacatat gaaaatcgct taccaactat
    15721 taagaagcat ttctcttatt ttgataaaat ggctgtttcc gatattgagc ctattgatgt
    15781 gcaaaattgg caactaaaat tatcaaagaa gtgtaagtct tcttatgtaa gagctgttca
    15841 aggactattt tcagttgcta tggatcgagc aattgtcctt gggataacca ccacaaatcc
    15901 atctaagatt attggcaatg tgaaaaagca aaagtctaaa attgaatttt ggacaaaaga
    15961 agaatttgaa aaagtaatct ctctaattta caaagaagat tactatcaac actttttatt
    16021 tatctctctg tggtttttat ttatgactgg aatgcgaatt ggtgaagcta ctgccattca
    16081 atgggaagat attgattttg actcaggagt actaaccatc aacaaaacac tctattacaa
    16141 gaatcaatcg aattatagat ttgttgaacc aaaaacaaag gcgagcgttc gtcatattgt
    16201 attagataaa tgcacattaa cttatttatc cgaatggaag gaagtacaac aaaatctaat
    16261 tcagacagac tttgtaatga gctataacgg tattcctacc caaaaacata cactatcata
    16321 tgctattgaa cgctatgcta agctggctgg tatccatcga atcagaatcc atgggttacg
    16381 gcattctcat gcttccttgt taatcagtat gggagaaaat ccacttatca ttaaagatcg
    16441 tctaggacac gaagatattg agacgacttt aggaacgtat ggacatcttt atcctaacag
    16501 taatttcgag gtagctaaca aattaaatgg agccatttcc tttaaagaag caaaacaaaa
    16561 ttatgatact tcaccaaaga atcaatttac tgtagactac ttgcgaaaca aagaaatgaa
    16621 aaaagtgcaa taaaagtgca gtaaaaagat aagaagagcc agaaaccctt ggtataagag
    16681 gatttctagc tctcctacaa ctattcccac tccactgttg ctggtggttt agaagtgatg
    16741 tcatacacga tacggttgac gtgggctact tcgtttacga tacgtacaga gattttttgc
    16801 aagacttccc atgggatacg ggcaaagtca gcggtcattc cgtcgattga agttacagca
    16861 cggataccga ctgtgtagtc gtaagtacgt ccgtcaccca taacaccgac agagcggatc
    16921 cctggaagaa cagtgaaata ttgccagata tcacggtcaa gtccagcagc tgcgatttct
    16981 tcacgcaaga tagcatcaga ttcgcgtacg atttctagct tgtcttcagt gatttcgcca
    17041 agaacacgaa taccaagacc aggaccaggg aatggctgac gccaaacgat tgaatcaggc
    17101 atgcctaact gagtccctaa ttcacggact tcgtctttga ataatgtgtt taatggttcg
    17161 atcaattcaa attgcatatc ttctggcagt ccacctacat tatggtgaga tttgatcgtc
    17221 tgtgctgttt ctgtaccaga ttcgataacg tcagtataca acgttccttg tgctaggaaa
    17281 gagacgcctt cttcaccagc aagttttgtt gcttcgtcat caaaaacata tacgaattca
    17341 ttcccaatga ttttacgttt ttgttctggg tcagatacac ctttcaactt gtctaagaag
    17401 cgatctcttg catcgacttt gatgatgttc aagccgaatt ttccgcctag actttccatt
    17461 acttgttccg cttcgccttt gcgtagcaga ccgtggtcaa caaagataga tgtcaattgg
    17521 tcaccgatcg ctttttgtaa aagaacccca acgacactag aatcaacgcc acctgaaaga
    17581 ccaagcaaga cttttttgtc accaacttgt tcacggattt tttctacttg catatcgata
    17641 aagttatcca ttgtccagtt accttcacag tgacaaacat caaaggcaaa gtggcgcagc
    17701 aattcgattc cgtattcgga atgacgtact tctgcatgga attggatacc ataaaaatta
    17761 cgtgttgtgt cttggataga agcaatcgga caatctttgc ttgttccgac tgtttcaaag
    17821 ccttcaggaa ctttagttac caagtcacca tggctcatcc atactgtttg ttgttttggt
    17881 gtttctttaa acaagacagc ttgatcgctt gttacttcta acaaggcttt cccatattcg
    17941 cggtttttag caggctctac ggaaccgcca agtttgtacg tgatcaattg cataccatag
    18001 caaatcccta atacaggaat acctaaattg aagatctcag gatcgatccc aaatgcattc
    18061 tcatcatata cactgtttgg tccgccagaa agaatgattc cctttggatt catcgctttt
    18121 acttcttctg cagtaatgcg gtggctcaat aattcggaaa agactccaag atcacgaata
    18181 cgtcttgtga tcaattggtt atactgacta ccgtagtcta atacgataat cttttcaacg
    18241 ttcgtcatat cggcaacgtt tgtcaaattt attaccccta tccctttaaa ttttatctca
    18301 aaatagtcaa actattttaa aatgctaata ttttcgcatg aaaatcgaat cgatgatatg
    18361 attttcagtt ttatggagaa ttatatcggc tcttcctcgt gtaggcaaga tatattcctg
    18421 taaatttttc aaattcacat ttttccatac atcttttgcc attttaaagg cttcttcacg
    18481 atctccgata gcaaaacgat aataataatt ctctggattt tggaaagccg tatccagtaa
    18541 cgcaccaaaa cgatccaaat accatttttc gatcagcacg ggatcggcat cgacatagac
    18601 agaaaaatca aaaaagtcgc tgacgtagat ttgctgattc gcaggtaatt gaagcgtatt
    18661 gatcccctca acgatcaaga tatcaggctg ctggatcaat tcgtacttgt ctggaatgat
    18721 atcatacaca tcatgtgagt aaacaggtac ttttatctct tcctgaccag attttacacg
    18781 gttcaagaaa tcgatcagca tctccatatc ataactttca ggaaatcctt tacggtccat
    18841 gattccacgc tcttttaata tacgatttgg ataaagaaat ccgtcagtcg tgatcagctg
    18901 aacgtttctt cgtttgaagg tacgagacaa aatcgtctgc agcagtctgg ctgttgtact
    18961 ttttccaacg gctacacttc cggcgatccc gataataaaa ggcggtactt ctacgtattt
    19021 atgaagaaat aaacctttac tcaagaacaa agattcaaat tctttcatat aaagataaat
    19081 cagatgagca agaggtacat agatatctcg aacatcctgc atggaaatgc ggtcattcaa
    19141 acttttgata ttatctagtt catcttgtgt caaaggtgct tgcccattac gataaaattc
    19201 tcgccattct tctcttgcca cacggtagta gttcattgga tcattcatat tagcctcctg
    19261 cgttctccca tcaatggaac tattttaaca caaaataaac gcaaacccca ccacttaaga
    19321 gagattgccg atctttttgt ataatcttta taatagaaag aaacggagtc ttattcttga
    19381 cttgcgcacg gatacataaa aggatagact gttgtcatga aagaaatgag aaggatggaa
    19441 gacatgacaa atcattacta ttctgaacgc ccagatacgg cgcacgattt tgaacaatgg
    19501 gcttttgagt taaaaggcag aacctttcaa tttgtaacag attctggtgt attttcacgt
    19561 gaaacagtgg attatggttc acgtgtcctg attgatgctt tcgattggga agagttacct
    19621 gatgaaggaa agatcttaga tgtgggatgc gggtatggac cgattgggtt agctttggca
    19681 tttgcgacac agcggtttgt tgaaatggtc gacattaatg aacgagcggt aagtctagcg
    19741 caaggaaacg caaagcgtaa ccaaatcgaa catgttgata tccatcaatc gaatatctat
    19801 gaagcagttc atgaagaaac ctatgcagct atcgtaagca atccgccgat ccgtgctgga
    19861 aaaaaagttg tacatgaaat tttgacaggc gcatatcctc ggttgaagaa gggcggtaca
    19921 ctgacaattg tgatccagaa gaaacaagga gcgccgagtg cgcaaaagaa aatggaagaa
    19981 acatttggta atgcagaaat cgtcacaaaa gataaaggat attatatcat caaaagtgtg
    20041 aaagaataac tagctgaaaa atagtggagc gacgaaagag tggaactggg cgtgcatgta
    20101 cgggtacgta tcagttcttt ttttgttgag aagtttttaa aaacaaagaa ctattttttc
    20161 ttcaaaaaga aatagtagga acagagtcgt tgttttttac gataaaataa ataagataca
    20221 gaacttgtat agaaggctgc caagtcggta gataaaggag gtttttatca aaaaacatac
    20281 tttccttttt tctctattct tttcttgggg gttttggtac actgataggg aatttagata
    20341 gaagggagcc aataggatga agctgaccat cagagatatc gcagaaatgg caggcgtatc
    20401 agtaacaact gtctcacaga ttttgaataa caaaggaagc agatttagtg ataagacaag
    20461 aaaaaaagtg ttgtcgatcg tgaatgaaca tcattataag cccgattatt tcgcttcaaa
    20521 tgtgatcaat cgacattcaa agaccatcgg aatgatcgtc cctgatgtca ctgatttctt
    20581 cttctccaaa ctgatcgaag gtgtagagag ctatttgaat cctttgggct atgtgatcat
    20641 gttatgcaat tctcgtcata gccaagaaaa tgagatcaag tatttacaag agctttccca
    20701 ccgctcagtg gacggaatct tgctggccac accaaatatt ttacctgaga aatatgcatt
    20761 agacagcggt ttttatcaaa aaatgccaat cattttaatc gatcgcggat tgaatcgtcg
    20821 ggataacgga cggcttatcg taaaggaata tgagggcgcc tatcaagctg tttcgctgtt
    20881 tattgaaaat ggccatacga agatcggcat gctgaaggaa accactggat actatcaatt
    20941 agaagaacgt ttcaacggat atcgtcatgc gctgaaagat catggcctgc cttttaatgg
    21001 gaaatttgtt gaacatggag agttgactgt ccaaggtgga tatactgcat caaaaaaatt
    21061 actcaaacat aaagatatca cggctatttt ttgcgggaat gatgccatgg cgatcggatg
    21121 ctatcaggcg atcgatgaat tgggcaaaaa aatcccagaa gatatctcag tcataggatt
    21181 cgatggatta aagctgtcag aatacatgat tccgcaattg acgacagtgc agcagccaag
    21241 ttttgatatt ggattttatg ctgcacgttt cctgatcgat acgatcgaat ttcctcaaag
    21301 aaaagttccg aacaaagtgt tcgaaacaaa attaattatt cgtgagagcg taaaagtatt
    21361 gacaaagggc taactcggcg gtatattgct tatgtaagca tttacaaagg ctagttagtg
    21421 ctttttttat ttttagtaaa ccagtttact aaagtggttt acctttccta aaaataatgt
    21481 ataataccga atgaagacaa ggaggatcag gcaatgcgaa tggtggatct gattgaaaaa
    21541 aagagggatg gaaacgagct gtcgaaagag gaaatcgagt acatcgtcac gaactatacg
    21601 aatggaaaga ttccagatta tcaagtcagt gcattattga tggcaatttt ttatcaagac
    21661 atgacaaatg aagaaatcac gaacttaaca ttggcgattg ctaattctgg tgatgtcatc
    21721 gatttgagtt ctcttgaagg aataaaggta gataagcatt cgacaggtgg cgttggtgac
    21781 acgaccacat tgattttagc ccccctagta gctagtgtcg gagtgacagt ggctaaaatg
    21841 tctggacgcg gtctaggtta cacaggtggt acactagaca agctagaagc aattcccgga
    21901 tttcagatcg agctttcgga tgaagaattt gtacgcattg ttaatgaaag taaagtagcg
    21961 gtaattggtc aatcaggaaa cttggcaccg gcggacaaaa aattgtatgc cttgcgtgac
    22021 gtcacagcga ccgttgattc tcttccgctg attgccagtt cgattatgag taaaaaaatc
    22081 gcggcaggag cagatgcaat cgttttggat gtgaccactg gagacggtgc tttcatgaag
    22141 aacatcgaag atgcaagacg tctggcaaag accatgacga gcattgggaa actggctaac
    22201 cgagaaacag tggcggttat ctcagatatg tcagaacctc ttggcgaagc tatcggaaat
    22261 agtctagaag ttgtcgaggc aatcgaaaca ttacaaggaa atggtccaga agatctagta
    22321 gaaatgtgct acgccttagg aagccaaatg gttgttcttg ctggcaaagc taagacaacc
    22381 gatgaagcaa gaacgttgct tcaagaagct ctggaatcag gtaaagcgtt agcaaaattc
    22441 aaagaaatga tccaaaatca agggggagac ccagcgatcg tggaacatcc agaacgaatt
    22501 ttgactgcac gctacacaat ggagcttcct gctaaacaaa gcggagttgt gtcaaaaatc
    22561 gtggcaaatg aattaggaat tgctgcaatg atgctaggtg ctgggcgtaa aaccaaagaa
    22621 gaagatattg atcacgctgt tggattgaaa ctgcataaaa aaattggtga cactgtgaca
    22681 aaaggtgagt cactattgac gatctatagt aacgacgaag aaatcacttc tgtcattgaa
    22741 ttattatata aaaatattga aattggtgaa tcggcaatga agccaacgtt gattcatgac
    22801 atcatcacag aataaaggaa aaaagcggac tttaccgttt taaagaaaag gggaaaagaa
    22861 atggaactaa accgaatgat cgaccatact attttgaaag cagacgcatc taaagaagct
    22921 gtgatgcaaa ttatcgaaga agcaaaaaaa taccattttt attctgtctg catcaatcca
    22981 gcatgggttg cgctagcgaa agaacaatta caaggtgagc cagtagccgt atgtacagta
    23041 atcgggtttc ctctaggagc aaacacaccc gagacaaaag catttgaaac aacagatgcc
    23101 atcaataacg gtgcagatga agtcgatatg gtcatcaata tcggtgcttt gaaatcaaag
    23161 gatgatcaac aagtccaaaa agatatcgaa gcagttgttg aagcagcaaa agatcgtgcg
    23221 cttgtcaaag ttattattga aacatcgtta ttagatagag aagaaatcat ccgagcttgc
    23281 gaaatagcaa aagctgcagg cgctgatttt gtcaaaacat caactggttt ctcaacaggc
    23341 ggagcaaagg tagaggatgt ccgtttgatg cgtgaaactg taggaccaga aatgggtgtc
    23401 aaagcttccg gcggtatcca taacgaagaa gaagcaatgg caatgatcga agctggtgct
    23461 acaagaatcg gaacaagcgc aggagtagca atcgtatctg gttcaactgg tgaaggctat
    23521 taattttttc aaaggagaag ctcatggcag aactcaaaca agaatggatc aacacagcta
    23581 ttgaagcgtt agataaagcc tatgttcctt attcccattt tccggtaggc gcttgtctcg
    23641 taacggaatc aggcaagatt tatcaaggca tcaacattga gaatgcatca tttggattaa
    23701 cgaattgtgc cgagcgcaca gcttttttca aagcagtatc tgaaggagaa aaaacattta
    23761 cgcatctagt cgtagcgggg cacacccccg atcctatctc tccttgtggt gcatgtcgac
    23821 aagtaatggc agaattctgt tcccctgata tgccggttac gctagtagga gacaatggtg
    23881 tgacgaaggc aacgaccgtt cgtgcactgc tgccttatgc atttactgaa aaagatctat
    23941 aactcatgtt cattcagtca gctgactgtt gaaaaataaa acaattattt taggaggctt
    24001 tacagaaatg aaaaaagcaa aattatttgg tttaggtgct gtcgcactag cagcagggtt
    24061 atttttaggt gcatgcggca acaacggctc tactgattca tccggtggca aaagctcatc
    24121 agacacaaca actgcagcat tgatcacaga cacaggtggt gtagacgacc gttcattcaa
    24181 ccagtcagct tgggaaggac tagaaaaatg ggggaaagat caaggacttt cacgaggaaa
    24241 tgatggcttc caatatttcc aatcttcaaa tgaatcagat tacatcccaa atattgacca
    24301 agcattgaat gctggattca aaacgatttt tggtattggt tataaactaa aaccagcgat
    24361 ccaagaacaa gcaacaaaca atacaggtac gaactttgtt attatcgatg atgtcatcga
    24421 tggattagat aacgttgtat ctgcaacttt caaagacaac gaagcttctt atcttgcagg
    24481 tgttgcagca gcttatacga ctgaaacaaa cgtagtcggc tttatcggcg gtgtaaaagg
    24541 tgaagtaatc gatcgttttg atgctggatt caaagccggt gttgatgcag gtgcgaaaga
    24601 attaggaaaa gaaatcaaag tattgaatca atatgctggt gacttctctg ctccagataa
    24661 aggacgttca attgctcaag gtatgtatgc acaaaacgca gatatcatct tccatgcttc
    24721 aggcggtaca ggtaacggtg tattccaaga agcaaaatca ttgaacgaat caggcgacaa
    24781 aaaagtttgg gttatcggtg ttgaccgcga ccaatcagac gaaggcgaat acacattaaa
    24841 cggcgaaaag aaaaacttca cattgacatc tacactgaaa gcagttggaa cagtagtaga
    24901 agatttggca caaaaatcag cggacggtaa attccctggc ggcgaacaca ctgtttatgg
    24961 attgaaagaa gacggtgttg gtctaacaga aggacaactt tctgacgaag cgaaaaaagc
    25021 agttgatgaa gcaaaagaaa aaatcatttc aggtgacgta aaagttccag aaactcctga
    25081 agaaaactaa aaccatttct atagccttgt tagacacatg tttgacaagg ctatggtttg
    25141 attagaacaa gaattgttca tttatccaag ctgccttgtg acagtttgga taagggaaca
    25201 actccctgaa gtaatgtttg tttcaaaaga agaacgctca ctaagcgtga atcttagtca
    25261 atcgttgccc tagatagaaa tcgttaggat gtgaaaaaaa ggtggttcaa gaaaatattg
    25321 tcatagaaat gcagaatatt accaaagaat ttggaacatt caaggcaaat gacaacatca
    25381 atttacaagt aaaggcagga gaaattcatg cgttgcttgg agaaaacggt gctggcaaat
    25441 ctacattgat gaacgtgctt tccggattat tagagccgac atcagggaaa attttgatgc
    25501 gtgggaaaga agtaaagatc acaagtccga ccaaagccaa ccaattaggg attgggatgg
    25561 tccatcagca ctttatgctt gttgatgcct ttactgtaac agaaaacatc gtgttgggaa
    25621 gcgagcctag tcgcgcaggg atgcttgacc ataaaaaagc acgaaaagag atccaaaaag
    25681 tttctgaaca gtatggattg tcagtcaacc cagatgctta cgttcgtgat atttcagttg
    25741 gaatggaaca acgggtagaa attttaaaaa cactttaccg aggagcagat gtactgattt
    25801 ttgatgagcc gacagctgta ttgacccctc aagaaattga tgaattaatc gtgatcatga
    25861 aggaattagt caaagaaggc aagtcgatca ttttgattac gcataagtta gatgaaatca
    25921 aagcagtagc tgaccgctgt acagttatcc gccgtggaaa aggaatcggt acagtcaacg
    25981 ttaaagacgt aacctcacaa caattagccg atatgatggt tggaagaacg gtttcattca
    26041 aaacgatgaa aaaagaagcg aagcctcaag aagtcgtttt gtctattgaa aatctagtgg
    26101 tgaaagaaaa tcgtggatta gaagccgtga aaaacttgaa cttagaggtt cgtgctggcg
    26161 aagtacttgg tatcgctgga atcgatggaa acgggcagtc ggagttgatc caagctttga
    26221 ctggtttgcg aaaggcagaa agcggacata tcaagctaaa aggggaagac atcactaata
    26281 aaaaaccccg aaaaatcact gaacatggtg taggacacgt gccagaagac cgtcataaat
    26341 acgggttagt cctagatatg acattatctg aaaatatcgc actacaaacc tatcatcaaa
    26401 aaccttacag taaaaacggc atgctgaatt attcagtaat gaatgaacat gccagagaat
    26461 tgatcgaaga atatgatgtt cgaacaacga atgaacttgt tcccgcaaaa gctttatcag
    26521 gcggaaatca gcaaaaagca attatcgctc gtgaagtcga ccgagatcct gatctgttga
    26581 ttgttgcaaa tccaactcgt ggactggatg taggtgcgat cgaatttatt cataaacgtc
    26641 tgatcgaaca aagggacaaa tacaaagcag tgttactgat cagtttcgaa ttagaagaaa
    26701 ttttaaatgt ttcggatcgt attgctgtta tccatgaagg agaaattgtc ggtatcgttg
    26761 atccgaaaga aacatctgaa aatgaattag gtctattgat ggcgggctat accttagaag
    26821 aagcacgaaa agagttagaa aagggggctg gcgcacatga atgatcgcag cgaaaaattc
    26881 cgtaatttat tagtgcctgt actttcagtt gttttaggat tgatcttagg cgcgatattg
    26941 atggctgctt ttggttttca gcccatccaa ggttattctt ctatgctaga tgcagccctt
    27001 ggttcgcaaa gagggatagg tgaaacgtta aggcaagcaa cgccgttgat ttttacagca
    27061 ttaggttttt ctgtagctaa ctcagctggg tttttcaata ttgggctttc tggacaagca
    27121 ctttgcgggt ggatcgtcag tgtctgggtg gcacttgcgt ttccagatct gccaaaagtt
    27181 gttttgcttc cgttatgcgt gatcttaggt gccttagctg gtgcgatggc agcggctgtt
    27241 ccaggtttac ttcgagcatt ttttggaaca agtgaagtga tcgtaacaat catgatgaac
    27301 tatatcttat tatacgtaag cacatttgct ttacatgaag tgatgcctga aagctatcgc
    27361 tcaagtttgg actcttccaa tatgatcagt caaaatgcct cactgcggtt accatggtta
    27421 acacaagctt ttggcggctc tcgcgtaaac tcaggattat ttttagcaat catcgcgctt
    27481 attttcgttt ggctgctgat gaagaaaacg actttaggat ttgaaatccg ttctgttgga
    27541 ttgaatcctt ttgcttctga gtatgcagga atgagtagta aacgtacgat cgttttgtcg
    27601 atggtcattt ctggcgcttt ggcagggctt ggcggtgtgg ttgaaggatt aggaacgtat
    27661 caaaacttct ttgttcaaac gacttcgcta tctattggat ttgacggaat ggctgtttcc
    27721 ttgcttggtt ctggatcagc aatcggtatt cttttatcag cgatcttgtt cagtattttg
    27781 aagatcggtg gacttggcat gcagacagga gcaggtgttc cgtttgagat cgtcaatgta
    27841 tcgattgctt tgattatctt ctttgtcggg atcaacttcc tgattcgttt cgtcatggct
    27901 aaagtcttcc aagggaaaaa acaagaagag atcgttgcga caatagaatc gaaaccaacg
    27961 aatcaaaacc atgaaggggg cgaactataa tgtcgactat cgaattaatt gccttgatcc
    28021 ttacttctac actggtttat tcaacaccgc taattctaac gtcactaggc ggaacttttt
    28081 ctgaacgaag cgggatcgta aacgtagggc tagagggaat catggtcatg ggtgctttta
    28141 gttccatcgt tttcaacttg acgatgagtc catcacttgg ttctatgaca ccatggatcg
    28201 ctattttagt gggtggcgtc gtaggtatcc tattttctct gattcacgca gtagcaacag
    28261 tcagtttgcg tgctgaccat attatcagcg ggacagtaat taatctgatg gcacctgctt
    28321 taggtgtttt cttgatcaaa gcatggtatg gtaaaggtca aactgataac atcacgcaga
    28381 atctggggta tttttctttc ccaggattag cagatattcc ggtcattgga ccaattttct
    28441 ttaaaaatac attcttacct gcttattgtg ctattttgat tgctgtccta gcttgggtga
    28501 tcatcttcaa gacgaaattc ggcttgcgcc tccgttcagt tggtgaaaat cctcaagctg
    28561 ccgacacatt aggtatcaat gtatatgcta tgcgttattc aggcgtactg atttctggct
    28621 tgttaggcgg tatgggcggt gctgtttttg ctcagtcgat ttccggtaac ttttcagctg
    28681 gtacgatcgt tggacaaggg ttcatctcca tggctgctat gatctttggt aagtggaatc
    28741 ctttaggggc aatgggtgcc tcattgttct ttggttttgc tcaaagcttg agtatcattg
    28801 gcgcacaatt accagttatt tcttcgattc cgccagtatt gctgcaaatt gctccatatt
    28861 tgctgacaat cattgtgctg gttgttttct tagggaaagc ttctggtcca aaagcaaacg
    28921 ggaagaacta tatcaaatca aaataatctt ttagcgaata aaaagggtgg gttacgggaa
    28981 gtgtttttcg tctcgccctt tttgttttaa aggcaataaa aataaaaaga aagaaggaat
    29041 aatcatgttt aaacgcgtac atctgatcgt catggattca gtaggaattg gagaagcacc
    29101 agatgccgat aagttcggtg atgttggttc tgatacatta ggacacattg cgaaagaagc
    29161 aggattgaac gttccacatt tagagcagct tggtttagga acgatccgtc ctcttgaagg
    29221 tgtggaatca gtgaaagatc atcaaggcta tgcgacgaaa ttagaagaag tttctgtagg
    29281 gaaagatacg atgacaggcc actgggaaat catgggattg aatatcaaaa ctccatttcg
    29341 tgtgtttcct aatggattcc ctgatgatct gatcaaacaa atcgaagaat tttctggccg
    29401 taaagtcgta tgtaacaaac cttatagcgg aacagctgtt attgacgatt acggtgaaca
    29461 ccaaatgaaa acaggcgatt taattgtata tacttctgcc gatcctgtcc ttcagatcgc
    29521 ggcacatgaa gaaatcattc ctttagaaga attatatcgg atttgccaat tcgttcgtga
    29581 tataacaaaa gatgatccgt atatgattgg ccgaatcatt gcacgtccgt acgtgggtga
    29641 accaggagac ttcacacgaa caagcaatcg ccatgattat gcgttgaatc cattcggaca
    29701 cacagtcctt gattctttga aagaagcagg aaaggacgtg attgctgttg gaaagatcaa
    29761 cgatattttc aacggacaag gaatcactga atctgtccgc acgaaaagca atatggatgg
    29821 cgttgaccag ctgctgaaag ttatgaaaaa cgatttttca ggaatcagct ttaccaattt
    29881 agtagatttt gatgcattat acggtcatcg tcgggatgta gtcggctatg cgcatgcgat
    29941 tgaagaattt gacttacgaa ttccagaatt gcttgaagcg atggaagaag acgacttgct
    30001 aatgattaca gccgatcacg gaaacgatcc gacgtttact ggtaccgatc atacgcgaga
    30061 atatgtaccg ttattggttt acagtaaaaa aatgaaagaa caaggggcat tgcctcaagg
    30121 atattattca gatatttcag caacgattgc tgagaacttt agtgtttctt cgactgaaaa
    30181 tggcgaaagc ttcttgaaat tattaaaata atctttttaa ataggagaaa gcaaatgacc
    30241 aagttaagcg agatgttaca agaaacgacc cagtttatcc ttgataaagg agtaaaagaa
    30301 attgattttg gtttgattct aggttcagga ttaggtgaat tagcagatga aatcgaagat
    30361 gctgtaatga tttcttatga tgagatcccg ttctttcctg tttcgacagt agttgggcat
    30421 gcaggaaagc tagtttatgg gtctctggca ggcaagaaag tgttagccat gcaagggaga
    30481 ttccactact atgaaggtca ttccatgcaa acagtgacct atccagtacg tgtgatggcc
    30541 ggactagggg cccattcagt cattgtgact aatgcatgtg gtggtgtcaa cgaatcattt
    30601 gtaccaggag atttgatgct gattaccgat catatcaatt ttactggtga aaatcctttg
    30661 atcggtccga atgaagatgc aatgggccct agatttccag atatgagtca agcttatacg
    30721 cctgactatc aagcaacagc aaaagcagca gcagaaaaac taggtttgga tcttaaagaa
    30781 ggcgtatata tgggctattc tggaccaact tatgaaacgc ctgcggagat ccgaatgtct
    30841 cggacaatag gagccgatgc agtagggatg tcgactgttc cagaagtcat cgtagcagct
    30901 cacagcggat taaaagtgtt aggaatctct tgtattacca acttagcagc aggtatgcaa
    30961 gcgaatctga gccatgaaga agttgtagaa acaactcaga gagtcaaaca atcttttaaa
    31021 gcattggtta aagaaacatt gacattattg taataggagg aacaataaaa tgagtgtgca
    31081 tattgaagcg aaagaaggcg agatcgccga taagatttta cttccgggag atcctttgcg
    31141 agctaaatat attgcggaga cttttttgga agatccagtt tgttacaatc aggtacgcgg
    31201 tatgttaggc tttaccggca aatataaagg agaacgtatt tctgttcaag gaacaggaat
    31261 gggaatgcca tcagccacca tctatgccca tgaattgatc cagtcgtatg gtgtaaagaa
    31321 attgattcga gtaggtacat gcggtgcatt gtcaaaagac gtccatgttc gtgatttagt
    31381 actagcacaa ggtgcagcga ccagttcttc gatgatcgaa aaaaatttcc aagcctttca
    31441 ttttcctcca atcagtgatt tcaatctatt actgaaagca tatgagatag ctaaagaaaa
    31501 gggctatacg gttcatgtag gaaatgttct ttcggaagat tctttctaca aagatgattt
    31561 gacagagacg ttccaattag ctgaacttgg cgttttagga gtggaaatgg aagcagcggc
    31621 tctttattac ttaggcgcga aataccatgt gcagacatta agtttgatga ccgttagcga
    31681 ccatttgatc accggtgaag aaacaacagc tgcagaacgt caatctacgt tcaatgaaat
    31741 gatcgaagta ggattggaaa cagcgatagc agaataagaa ggaaatgaaa aagctgccct
    31801 gcattaagta ataagaacat ccaagcaaaa atagctcctc atgtttttga cttggctgga
    31861 cttattattg caaatgtaag cttttgaaca ccgtttacca agaggttgtg aatctgccgt
    31921 ttaactctga gtcacaagga acaatttcaa aaaacagctt ttcatgtttg atgaatattg
    31981 tgacttgtga ccgagaagtt ggcaggagaa aaccgtttat acgaaaaaag ggactgtgac
    32041 aaatcgtgtc acagtccctt ttttatagtg ttttttatag gtagtatttt ttagcaactg
    32101 ttaagtcatc atttaattca taaacaagtg gttgacctgt tggaatttca agatccatga
    32161 tgtcttcgtc agagatacct tcgatatgtt tagctagggc gcgtaatgag ttaccatgtg
    32221 cagctactaa aacagttttg ttgtctagta atgctggagc aatttcatct tgccagaatg
    32281 gcaaagcgcg ttctaatgtc actttcaagt tttctccacc aggaatatcg cgtttgtcta
    32341 acattgcgta acgacggtca tttgctgcag aaccttcgtc agtttcttcc attaatggag
    32401 gaagagtatc gtaagaacga cgccagatgt gcacttgttc gtcgccgtat ttttcagctg
    32461 tttctttttt gttcaaacct tgtaatttac catagtgacg ttcgtttaag cgccatgatt
    32521 tgatttgtgg tacccaaagt tgatctgaat attctaagat caagttacat gttttgattg
    32581 cacgagttaa aacagaagtg taagcaacat caaattcgat tccagcttct ttgattttgc
    32641 gtccgccttc ttttgcttct tcgattcctt ctggtgctag atttacatca gcccaaccag
    32701 taaattggtt caatgcattc cattcactaa gaccgtgacg agaaaaaact aattttggca
    32761 tgtttagctc tccttttaag ataggtttat atttctgaaa ttttcagttc tgttttattg
    32821 tacaattttt tggaagagat ttccatcga
//
LOCUS       DAYYCY010000013        32261 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000013 DAYYCY010000000
VERSION     DAYYCY010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32261
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.013"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1872
                     /locus_tag="ACHAZB_000494"
     CDS             <1..1872
                     /locus_tag="ACHAZB_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HFM8613197.1"
                     /translation="SAENMEEIKKMFPKSTWFGFTGTPIFPENRKQAKGQLARTTHDQ
                     YGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENKIYRKLKEVETYAEYSSEQIN
                     RMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDNAYTKFDFRNGRPTKSAILTT
                     SSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTMEDPDFPRIAITYSLEENSRD
                     SSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNRLARKRAEFKQFGKQIDLAIV
                     VDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTYPDKTKGLIVTFRKPATMEKN
                     VEDATKLYSKAKEESGLIYPSYQESKKRFKQAYGKLKEVSSMESIDEHTPLEIRIEYV
                     KAFQELNRAYESLVTYDDYNDDMVKSPTLNNQIQLLEEQIGVYETVKGSLIEEEPEKE
                     GEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSDAREEIEKALAKLNKTEGVKE
                     VYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHVFSNEWFVSEKELHASNLQYL
                     PGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMKRAWRKMLDDELIPLENELR"
     gene            complement(3807..4472)
                     /locus_tag="ACHAZB_000495"
     CDS             complement(3807..4472)
                     /locus_tag="ACHAZB_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8613198.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            4598..6634
                     /locus_tag="ACHAZB_000496"
     CDS             4598..6634
                     /locus_tag="ACHAZB_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HFM8613199.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            6638..7276
                     /locus_tag="ACHAZB_000497"
     CDS             6638..7276
                     /locus_tag="ACHAZB_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8613200.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            7304..7732
                     /locus_tag="ACHAZB_000498"
     CDS             7304..7732
                     /locus_tag="ACHAZB_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8613201.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            8189..9100
                     /locus_tag="ACHAZB_000499"
     CDS             8189..9100
                     /locus_tag="ACHAZB_000499"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM8613202.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            9317..9754
                     /locus_tag="ACHAZB_000500"
     CDS             9317..9754
                     /locus_tag="ACHAZB_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8613203.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            9849..10733
                     /locus_tag="ACHAZB_000501"
     CDS             9849..10733
                     /locus_tag="ACHAZB_000501"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFM8613204.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            10957..14124
                     /locus_tag="ACHAZB_000502"
     CDS             10957..14124
                     /locus_tag="ACHAZB_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFM8613205.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            14192..14689
                     /locus_tag="ACHAZB_000503"
     CDS             14192..14689
                     /locus_tag="ACHAZB_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HFM8613206.1"
                     /translation="MILLLGFILWQTPVHASEFNFAVDFELPANQIDKNKTYINVRME
                     PGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESKSKFDETLSYDMKNLAK
                     TVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKENVSNKSTVGVVNQFSQV
                     KAILI"
     gene            14708..15172
                     /locus_tag="ACHAZB_000504"
                     /pseudo
     CDS             14708..15172
                     /locus_tag="ACHAZB_000504"
                     /inference="COORDINATES: protein motif:HMM:NF023225.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
     gene            15169..15927
                     /locus_tag="ACHAZB_000505"
     CDS             15169..15927
                     /locus_tag="ACHAZB_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFM8613207.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(16013..16753)
                     /locus_tag="ACHAZB_000506"
     CDS             complement(16013..16753)
                     /locus_tag="ACHAZB_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFM8613208.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            16814..18067
                     /locus_tag="ACHAZB_000507"
     CDS             16814..18067
                     /locus_tag="ACHAZB_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFM8613209.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            18389..18616
                     /locus_tag="ACHAZB_000508"
     CDS             18389..18616
                     /locus_tag="ACHAZB_000508"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFM8613210.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            18739..19065
                     /locus_tag="ACHAZB_000509"
     CDS             18739..19065
                     /locus_tag="ACHAZB_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFM8613211.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            19133..19846
                     /locus_tag="ACHAZB_000510"
     CDS             19133..19846
                     /locus_tag="ACHAZB_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFM8613212.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            20156..21007
                     /gene="licT"
                     /locus_tag="ACHAZB_000511"
     CDS             20156..21007
                     /gene="licT"
                     /locus_tag="ACHAZB_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFM8613213.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            21128..22993
                     /locus_tag="ACHAZB_000512"
     CDS             21128..22993
                     /locus_tag="ACHAZB_000512"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFM8613214.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGKPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            23007..24450
                     /locus_tag="ACHAZB_000513"
                     /pseudo
     CDS             23007..24450
                     /locus_tag="ACHAZB_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738852.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
     gene            24558..25259
                     /locus_tag="ACHAZB_000514"
     CDS             24558..25259
                     /locus_tag="ACHAZB_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8613215.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            25385..25897
                     /locus_tag="ACHAZB_000515"
                     /pseudo
     CDS             25385..25897
                     /locus_tag="ACHAZB_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014566402.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(26295..26504)
                     /locus_tag="ACHAZB_000516"
     CDS             complement(26295..26504)
                     /locus_tag="ACHAZB_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit beta"
                     /protein_id="HFM8613216.1"
                     /translation="MYYFKQQILVSFINEKIILFSCYVSLINDVSLNKKADELNDQNL
                     IIFVHQLLLKIFLLTINSWICKNIT"
     gene            26745..27320
                     /locus_tag="ACHAZB_000517"
     CDS             26745..27320
                     /locus_tag="ACHAZB_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HFM8613217.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            27320..27760
                     /locus_tag="ACHAZB_000518"
     CDS             27320..27760
                     /locus_tag="ACHAZB_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFM8613218.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            27776..28477
                     /locus_tag="ACHAZB_000519"
     CDS             27776..28477
                     /locus_tag="ACHAZB_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFM8613219.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(28965..29390)
                     /locus_tag="ACHAZB_000520"
     CDS             complement(28965..29390)
                     /locus_tag="ACHAZB_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8613220.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            29764..30735
                     /locus_tag="ACHAZB_000521"
     CDS             29764..30735
                     /locus_tag="ACHAZB_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8613221.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            30767..32164
                     /locus_tag="ACHAZB_000522"
     CDS             30767..32164
                     /locus_tag="ACHAZB_000522"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8613222.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
BASE COUNT        10968 a         5224 c         6634 g         9435 t
ORIGIN      
        1 agtgctgaaa atatggaaga aatcaaaaaa atgtttccga aatccacttg gtttggcttt
       61 acaggtacac cgatttttcc tgagaatcgg aaacaagcca aagggcaatt ggcacgtaca
      121 acccatgatc agtatggaga ggtcttgcat acctatacca ttaagaatgc gctagaagat
      181 ggctctgtct taggatttca agtagagcat gaaaacaccg tggagccgac ttcactagag
      241 aataaaatct atcggaaatt aaaagaagta gaaacatatg cagagtattc atcagaacag
      301 atcaatcgta tgattgatca aatggaacct gtcaaaaaag aaagttattt ggacccagct
      361 gtttttgaag cagatgaaca tatccaaaaa gtcattcata aaatgttccg tccagataat
      421 gcctatacaa aatttgattt tcgaaatggc aggccaacga aatctgcaat cttaaccacg
      481 agttccattg acatggccaa aaagtattat cgggccatca aagagatgac gaaagagcct
      541 gattggttaa caaaagagtt tcccaatcag ctcattcgtg aaggacgaac gatggaggat
      601 cctgattttc cacgaatcgc tattacctat tcattagaag aaaatagccg agattcaagt
      661 gcccaacaag aagaaatgca aaaaattatt gaggaatata atcagtatta tgatacttcc
      721 tggtcattag cagacattga gcgttataac ggagacatca ataatcgttt ggcacgaaaa
      781 agagcggaat tcaaacaatt tgggaaacaa atcgatttag cgattgtagt ggatcgtcta
      841 ttaacaggtt ttgacgcccc tacgattcaa accctatttg tggatcgtaa tctagagtat
      901 gcaggattga tccaagcttt ttcacgtacg aatcgtacgt atcctgataa gacaaaagga
      961 ttgattgtga cgtttcgtaa gccagcgaca atggagaaga atgtcgagga tgcgacaaaa
     1021 ctgtattcaa aagcaaaaga agaatcaggt ttgatttatc caagttatca ggaatccaaa
     1081 aaaagattca aacaagcata tggtaagtta aaagaggttt catccatgga atccattgat
     1141 gaacatacgc cacttgaaat ccgtatcgag tacgtcaaag catttcaaga attgaatcgt
     1201 gcttatgaat cgttagtgac ttatgacgat tacaatgatg acatggtgaa atctccaact
     1261 ttgaacaatc aaattcaact tttggaagaa caaattggcg tgtacgagac cgtgaagggt
     1321 tcattaatag aggaagaacc agaaaaagaa ggagaagatt tttcaacgat cgaattttat
     1381 agtgaaaata ccactaagtt gtatgatatt gattcaactt atattgatca attactagga
     1441 acttatgctg cgaatagttc agatgcacga gaagaaattg aaaaagcttt ggctaaattg
     1501 aataaaacag aaggagtcaa agaagtttac cgacaaattt taaatgcatt tgatgtgggg
     1561 actttagata gtaatgaaga tatctttgtc attaaacgtc gctattttac acaagcgagt
     1621 gatgatttga ttcatgtctt ttccaatgag tggtttgttt ctgagaaaga attacatgct
     1681 tctaatctgc aatatctacc aggtgaagat ccaattccta atatgaaagc aattatcaac
     1741 agtaaggatt acgaaggata taaagccaaa catccagaag cgaagccatt taaataccca
     1801 caagaaatga agcgagcatg gcgtaaaatg ttagatgatg agttgattcc acttgagaat
     1861 gaattgaggt aaacgagaag agttaagaaa attgtacgat tcaaagatgg tagtcaagtg
     1921 gaagtgtaga aaatagggat agatgtcttt tatttgttca cattttataa cgaagtattt
     1981 aagacagcta ttgaataatg tttcctccac ctttttcatt catttacccc accccgttgt
     2041 tatatgaccg tgttttgaga ggaaccaata aaaagcatag gctaactagg ggttcctttt
     2101 tcttttgttt ttttggaatc cgcttaagtg aaatgtatct gaaatgaaac ggagataaag
     2161 tgattttttg ctataatgac aaggttttag ctgtttcaga taccatgtaa cccaaaatag
     2221 tttattgaga aaataggaga gtatttgata ctaactagta ggaaaaaatg agataaaaat
     2281 taaatggaat catttcaggt aatctcaaat aaaaaaatat acagtcctgt ggcactttac
     2341 ctatgggact gtatattttt tactctcagc gggcttgttt tgactccgtg tctgactccg
     2401 tcgtattgat tttaaatgat attcgatgac catttaacag gtcatttttg aaatttttgt
     2461 ctctatatga ctcagtttga taattattga tagatcaatt tgggtctata tcgaacaagg
     2521 gaataatcca ataaaaaatg atttagaata tcaaaataac aaaaaagagg actacctgta
     2581 ttcgtcctct tttttgacca gattttgacc agtctaattt acattctaat atattcatat
     2641 gagctaattt tttgattaaa tggtaagttt ttccttgttt gttttagttt aaactagtgt
     2701 gtaatttaaa aatctatatg gaagggtctg tggggtaata cttaaataaa ggctaggaac
     2761 ctgataacat caaggttcct agccttttta taataaaatg aagcgaaaat aatttctttt
     2821 aaagagccga gattatcagc tatataagag tgaatttgat gcaaaaaaca gagccgaaac
     2881 agtcttggtg ttcgtagctc tgcttatatg tttcgaattt aagacaaaga gttggcaaaa
     2941 gatgttgata aggaagataa gataactgtg ttatataaac taggtaacta actgtaacaa
     3001 gctaatttta agagattctt attagaagac gtttaggaat ataactaaga aaatgattat
     3061 acaggtaatt gtcatttcaa taatgttttc taattttcgc gtctttgttt taagaaaaag
     3121 gccagaatca gttggaaaaa agttcgtacg atagtgaaga ggggcaaaat ttcctactaa
     3181 tattcccact aacaagagat aaaacggcca ggttggtgtg attcctctat aaaaaaatgc
     3241 ccatgctacc gaaatcccat agattaacaa ctgaacaatt tttacaatga aaaaattgtt
     3301 ctgccattac tcaaagtctc ttcctccttg tatcgtttat ttgtttcatt ttatcattat
     3361 gataggcgct tgaacaatcc attttgtatt ttattgagta ctcttggata aactaccagc
     3421 gattatcata tttatttatg ttactgtaag ccatcatata aaagtaaatg agcagatcat
     3481 agaaatataa aaaagaacta aaattgatct aaattatgtg cagcttgact acgtaccaat
     3541 ctatattact tactagattg gtcgttaatc tattcggatc gaatgagcaa aatatttttg
     3601 tgaatgtcca tccttgatta tgattccaaa aagaatcatt gggaggctgt ggcgtaaata
     3661 ttatactata agccagaaat acctatcatt ctgaactttt ttatttagga actttcatct
     3721 caaggctttt tcaattaatt tgtacccctg ttttttggat aagatcatgt gcattgattt
     3781 tttctatctc tacgttcatc tcctacttaa tgcctatctg gctcctgagg gcttcttttt
     3841 aaggcgtcgt catagtcttg gctgaggatt tcttggtaat ctgagggaat ggcttcccaa
     3901 gtgagcaggc gaacgttcat gacgtgtttt cctttgtgtt tgattacagc atggtttttc
     3961 tctttttcta cctctccagc gtgaaaagac agccatgtac catggccaaa gatatcatta
     4021 catacccaac gttcgatgga attgtccacg tatcctacag ggagtgtggt atagccgatt
     4081 tgtggtttca aaaaaggatt gaatcgatcg atcgtttcat ttttgtattg ataattgata
     4141 agaaaaagat agaccaaaca aaagacgagc agaaagaaag atagtgtctt tttgagtgag
     4201 aagggttgtt ttgatgaata agatttcttg ttaacaagag gttcatctgg caatgttggt
     4261 tctattgatt cggtagctga ttcaatagaa ggaaaatcaa tcggcagacc taaagtatga
     4321 tgtaaaagtg acagcatttc aaggtctggc attgtacgag aattttccca actagaaatc
     4381 gttttatcgg aaacaccaat tttttcgcct aattcttttt gcgtaagctt acaatttctg
     4441 cggtaagttc gtattttttc tccggtgttc atcttcttct cctttcgatt ctacagtata
     4501 tagatttatt ctacatgacc tatacacttg atagatacgt ttttttgatg aaaaacggta
     4561 tgattttatc aaagaatcat ttaagtaagg gagagtggtg ataatcaaaa aaaagttcac
     4621 agttttactc acaatcatag tgaccggtat catgagtggt tttgccttgg ttagtataaa
     4681 taaacaggta ctctatcgga caaatgaatt tttccatcaa acagatactt atactccgat
     4741 tcgattgcca caacatttta ttgggcaaga agaatatgag cagctaatca tggtatttga
     4801 aaatgtctca tcagaaactg agctttctta tatgaaaaaa acagtaaatg aatccaatga
     4861 agtagataca tcttggaact tcaagataca gccaaagctt gaaatgacat tgtattatca
     4921 aggggatgtc aaatcttctc acgagaaggt accctattct aatacctcct ttcgcgtaac
     4981 gtccattgaa gaatcgatca aaataaaaga ttttgaaggt gaatttttct tgatgacgag
     5041 agaaaaagaa gcctatgaac ggttcatttc actattcact gatcgattca atcaagcatt
     5101 tcacactgat tatgccacta atatttttga agactttgaa aatagctatt cgtattcggt
     5161 tatttcaaat acagacgtat ataattatcg agtgtattta actattggat tgattctttt
     5221 attccttttg ttatcatcct ttttcttatt gatgaataaa gaaatcagtt tattgttgaa
     5281 taatgacttt tcattacatc aagtattgtg gtgtttattg gggaaaaaga aaatcattat
     5341 tacattcata attgcaggaa tcattggtgg ttttattttt atgtattata aggagcaatt
     5401 tctttatttg ttccagatgt acgttttctt gttcagcgga atatatggta tctctttgat
     5461 ttctctttta ttagttgaaa aaatcgcttc gttaagagaa gcgttcaact atatccaata
     5521 cattcttttt acatttggat tgttgttttc tcttcttttg accgttgcca atgtcgaatt
     5581 agcaactatc gttttttctt cttcacagtt agctgtttcc catgaaccac caaaagaaat
     5641 cgctggtgag gactatcatg tattttatcc agtaaccatc gggaaaaacc acgtggattt
     5701 tatttattcc aatcgttttg catcagcagc agatcaggaa ttatatgaaa ctttgaataa
     5761 aaacggaagt atattagtca atgtatcaga ttacttgaat gaagaaaatg aacagtttgg
     5821 cagagggata aaaataaatc tgaattatct taagaaattt cctttgattg atgtctctgg
     5881 aaggttgatc caaataaccg aaaaagaaac gcaccctgtt atattgatcc ctgaacgata
     5941 tagacataca gatttaaaaa atgacctggc gcaaataaca gaatattatc aagaaatcag
     6001 tgaaaaagaa ccgaaatttc tatttataaa aaacaatcaa cccatttata catttattcc
     6061 ttctatcccc tggatcgaac attatccggt tgttgaaata ctgacattga aaaacagtat
     6121 gtatttggaa agaaatattc tcagcggaga aatctatcca ccgttaaaga tcaagatagg
     6181 ctctttgtcg aaagaaaggt tatttgagct gattgaagaa agtcagttac gtgataactt
     6241 gcagttatca tttccgtata ctcagactga agagatagct gtcaaagtgt taagcagatc
     6301 atttcattac ttgatacaga tatttttact cactttcttt tctttcttct tgttgagcta
     6361 tgtcatgtta tgcatatatt ttgtctacaa ttgtcggaaa atcgctatct ttcgttcaag
     6421 tggttattcg ctctttgaaa cgtataaaga tttcttcatg atgaacctca tcaaatgggg
     6481 aacgacaagt gtaatcttcc tttttcttat agaaagagag cctaaatatc tttttaatat
     6541 ttttttcttt agcttcattg attttattct ttcagttgtt tttattttgt ctacagaaaa
     6601 gaaaagtcag ttattgctga tgaatggagg ttaacaattg gagccgttga tagtgataaa
     6661 aggtgcgaaa aaaatcattc gaaacaaaac gattcttcat ttggatcgtt tcactgcgga
     6721 tgctggggat cttgtgactg ttgttggaga aagtggagca ggtaaaagta cgttattgaa
     6781 tatattagga ttgaatgatc agttcagttc aggacaattc tatttgttcg atcaagagac
     6841 gaaacaatta actgggagag agaagcgctt gataaaaaga aataccatat cttttctttt
     6901 tcaagatttt gggttgattg aatatgaaac aatcgatttt aatttagata ttggattgaa
     6961 gttttcgaat ttaaatgttt cagataagcg aacagcaaag tatgaggcat tgaaaaaagt
     7021 aaatataaac aagaagctaa gtacgcctat ctacacatta tctggtggtg agaagcaaag
     7081 aatcgcctgt gcgagagtct tattgaagaa tagcaaaatc attttagccg atgaaccgac
     7141 tgggtcattg gataaaagaa ataaggataa agtagttgaa atcttatcgc aacaagcttc
     7201 ccaaggtacg ttagttatcg tagtcacgca tgatccatat ctgaccaaaa taggaaacaa
     7261 gaagatcatg ctttaaaaat caaaaaggaa aggatgtcgt tttatgaaaa aagcagttat
     7321 tatttgtgca ctagggatag gttttgttgg aggaaacgct atgtcatctg aagtggaagc
     7381 aaattcagtt cacggtgaaa tctattacaa atctttaaag ccaggagaga ttcattttga
     7441 tgctaaaggt aatattatta agtcaaattt accagtagac cgggaaattt ctagaacagc
     7501 aattaatcat agctctggaa gatgggttta ttatagtgat acaacttgta cgagaaaaac
     7561 aggtcattct aaccactata gtcgtgtcta taatcgtcac ggtgcaaaag cgaaagttgg
     7621 aaactctatt cgatcagcaa atgcaacaaa aggagtatgg gcctatgcaa gtgcgacagg
     7681 aaatagaact gacacattct catgttggta caatccaacc ggttggtatt aagtatccaa
     7741 aagtttatat aaaatagaaa aaacatataa tgaaacagcg ttaaaagtta tttttggtag
     7801 ttttaatata ttcattttta gttgtataaa aatatatcat aataaaaaca acaaaaaaat
     7861 gtctggaaaa tcgaacataa aagtgtctga tttgaatatc ccagcagcgg aagaagaaaa
     7921 acaaaatcga taacatttgt tctaagaatt agttacgtta aatacggaac gcaagataga
     7981 cttgaaaaac ggtaacaaaa gaagtagtat attaaggtgt gatgtgctac ttgacattta
     8041 ttctgctgag cgaactttct ggattgtgta gccttatatc tattcaggac gtatcgagca
     8101 gattagtaaa ggtgttcttg atgattttca tcttgaacac ctttgttttt agcaattcgc
     8161 aaaaaacgaa aagtaggaga attgataaat gaaaaaaaga tttttagcac ttgccatcgt
     8221 actaggcact ggcttattgt caggctgtac gaatgccggt gaaaaaacag ccgttagtta
     8281 taaaggcggt acaatcagcg aacaagaagt aatggatagc ttgaaaaaaa tgcaaggagc
     8341 agattccgct gtgcaacagt tgatcgttta tcaagtattt gaagataaat atggagatga
     8401 cgtatcaacc aaagaaatcg attctcaata tgatcaaaca aagaaacaat tgggtgattc
     8461 atttgattca caattgaaat ctgcaggtta cacagaacaa acattcaaag acagtatcaa
     8521 acaaagtcta gcctttcaag aaggtctaaa gaaacatatc aaattgaccg atgaagattt
     8581 aaaaacggct tgggaatctt tccatccaga agtggaagct caaattatcc aagtagcaag
     8641 tgaagatgat gcaaaagatg tgaagaaagc tgctgataag ggagacgatt tctctaaatt
     8701 agccaaagac aagtcaactg atactacgac aaaagaagat ggcggaaaag tgaaatttga
     8761 ttccactact acaacggttc cggcagaagt caaagaagca gcctttaagt tgaaagatgg
     8821 tcaagtttct gatgtgatca cttctaccaa tgcttcaact tatactacgg aatattatgt
     8881 ggttaaaatg gtgaaaaacc aaaacaaagg caacgacatg gataaataca aaaaagaatt
     8941 gaaagaaatt gcaacagaca caaaattaag tgacagtacc ttccagaata aagtaattgg
     9001 cgaagtattg aaagacgcta atgtaaaaat caaagacaaa gactttgaaa atgttttgag
     9061 tacattcact tcagattcta gtacagcaag tagtaaataa gcggtataaa taaaaaatat
     9121 gaactattta aatcaataaa actgttcatc taaatctttt tattagaact aaatcaacag
     9181 tagtttaatt actacttgat ttagttcttt ttattttaaa aatttttttt acttagaatc
     9241 cagcgaccct agatattttt ctgtataatc ttaaataaag aaaacaatat caaaaaatgc
     9301 acgtagaagt agataaatgg agtggttttg caaatttctt ttatttctag aagttattca
     9361 cgtatacgga tttgtttttc tcacaaataa aaaggcagga agtacaatgg atcatttgta
     9421 tataaaatat attcatcaat tttccgtttt ggtgttaggc acactaatta tagcggggat
     9481 tgtcgcattt attattcaag gccgtgacat attaacagta catcaagagt ttttcagtta
     9541 tgcatttggt attcctctga tcgctataag gtctgataac aaaaagtgga agaaacgcta
     9601 ttacattctc ttgctatttt tgtgttttta tctgccacct aagattttta ctttattagg
     9661 tatacaatgc ctatcatttt cttttatttc ctatatcatt gttttaattt tcctcagttt
     9721 catagtatgg atgttttatc ttgtcaaaaa ataaatgaat gtttcatgaa cagtacttca
     9781 tcacagccta atttttttct ttatgctatc gttatattaa agaaatcaat cagaagggaa
     9841 agaaagtgat gaaaaaagtt ttattagtgg taaaccccag ttcaggcggt gaacaagcaa
     9901 aggaatttga gcaattagcg atagcaaaat tggaatcggt gtttgatgaa gtggtcgtct
     9961 tgcatacaaa aaaagcaggg gatgcaaaaa actttactcg cgaagcggct acggaagggt
    10021 atcatagtgt gtttgtcatg ggcggagatg gaacagtcaa cgaaggaatc agtggaatcg
    10081 ctgaacaaga acatcggcca aatttcggat ttttcccatt gggtacggtg aatgacttgg
    10141 ctcgagcatt agggataccg ctagaacctg aggaagcgat caatcatttt tcgattgaat
    10201 cagtcaagcc attggatatt ggaaaaataa atgacgacta ttttatgaat gtcgtcgcaa
    10261 ttggctctat ccctgaatca atcaatgatg tagatgcgga gaagaagacg aaatttggaa
    10321 aactagctta tttcatgtca ggaatcaaac agttagcttc cacacaatcc tactcgtttc
    10381 acgtagaagt tgatggaaag aaggaagaaa ttgagagcag taccttgctg ataggactaa
    10441 ctaactcagt cggcggtttt gaaacattac ttccaaatgc aaaagtggat gatggtaagc
    10501 tccatttggt ttatttaaaa gattcttctt tgatcgatac gcttaaaaca ttgccagacc
    10561 tattaaaagg agtagatgaa tctacgaaaa atctagccta ccttacttgt gagcaattag
    10621 ttgtgtcatt agctgatgag gaagaattga cgacgaatgt tgatggagat gaaggggata
    10681 aattacctgt gacaatcaaa gtattgccct cacatttaca ggtttattgt taaaatggag
    10741 gttgtgaccg aggagttggc gggagaacac cgcttgttcg ggataaggga ctgtgacaag
    10801 acttttgtca cagccttttt tttgtacaaa aaagaaattt acacctaata gaaaaatttt
    10861 tccataaaaa tacatatttt aaatatgaaa gtacaattgg aaatagatta aaacgataaa
    10921 ataaaattgt caaaacgtca gagaggatgg tggagaatga atcaatgtgt aaaaaagctg
    10981 attgcgtcga ttttgtttgt cttccctctc ttgatggtga catccgtttt tgccgatgta
    11041 gagaaaacaa acgaatcaac gcaatcagag aatcaaagtt ttgacaagac tactttttca
    11101 gaagaatcga ttacttctga aactacagaa tctcaaagtg aggcttctac taataaaagg
    11161 caagaagaaa ataagacaag tgagacctca gaagtagctg aacaaacgtc tgatagcagt
    11221 gaagcggcac tggcaagagc tgtttcagcg gatgggtaca gcgaagctgc cacaacagaa
    11281 gaactggcgc agcttctcgc agacgaatca gtaactaaga tccgactgat tcagcctttg
    11341 actttagaca gggaattaga gatcaaacga gatatcgtta ttgattttgg tggattcgct
    11401 cacaatttcg gcacacacca tatttatatc aatgagatac ctcagtcgat cgagttccag
    11461 aattttaaag gaacagctgc acatccaggt ggattgacac caaatatcga tggaaatgcc
    11521 attatcattg cttatatgac cgatttttgg ggaagcagat atcattttac gggagaaata
    11581 aaatttaccg ggacacttga tttgtatgat gggtccaaat taggactgat ttacgctcct
    11641 cgtgcaactg tcacattaga tggtgtttcc ggtgtgttgg atgtccaacc agtgaaggaa
    11701 ggactgaatg ccgctccggg taaagcctat ttttgtagaa gctatcaatt gaatgtgatc
    11761 aatggtagtc aactttatgg accgtatcta gggaaattct acggttttct agattcagga
    11821 gatgagactg gtagtggaaa aacagcgcca ggaatccata tcttaagcgg ttcaaaggtc
    11881 tctcttgatt atgatcgggc agatggcgtt agcgatgaag gagaggcagt cgatacgcta
    11941 cctggtaatg tcatgtttga agtcagtggt tcgggaagcg aattttcggt aaatacaaag
    12001 atcaatatta ccaatgacaa caatcgcgga atcatccaaa tgagaggttc aggatcgaaa
    12061 gtcactgtat cgaatggtgg gaaaatcacc atcaatacag caactacggg cggatttcgc
    12121 ttgcaaggag attctagtca aatccatgta tcaagtggtg gacaaatctc ggtcaaaatg
    12181 gctggagatg acgatcaacc cgggaacaat gggatgcgtt ttgtaggaaa aggcctttct
    12241 ttgatcgtag aaggagccgg atcagttatc gacatcgata agcagagtgg gcgttccagc
    12301 gcagttcgtt ttgagaatgg tacgcaaaag ctgacagtaa ccaatggagg ctcattgagg
    12361 gtaagaaata caggtgatgg caaatcttac gtgaacagac tcaatcaagg aaatcaagcg
    12421 atccaatttt atgacgcttc tggttttcta gaaagtccag gttcagctga ttttacagtc
    12481 actggagaaa aatcaaatat cgatatacgc gcagattccg gagcaactgt agatacaact
    12541 ggagatattg atttaaattt tacggcaggt gaaaaaactt atttagtcat ggtcgggaaa
    12601 acaggggaag ataagaccgg catcatcagt ggggatgtgt tgaatgtgga cttagaatct
    12661 cctacctatt ttgatttcca aaacaaacgg attggaagcg agaccaatgg cataggcggc
    12721 tgggtattcc aaggaaatgg agattcttct ttaaagatcg cccattcaga aatcgcttta
    12781 tggcgaaaaa caggcgcaac attcgataac tttgaaaaag acccggatta tttttctgat
    12841 atgacagact tatcgatcaa tgggaccgac ttaggcaatt tcgtatcaag ccctagtgca
    12901 ggtttaacaa atgaatttgc tcagacaggt gccggaatga aaaattataa tcgaatcagt
    12961 gccaataatc agctcccgat gattgaatcc ttaagagtgc caacgaatgc agataaaaaa
    13021 atctatgggc atgtcgttgt tcccgaaggt gtcgaggcaa agccaagaga tgcttggaca
    13081 ggcgaagtag aagtgacttt acgtattact tacgcagata cgacgaaatc atctgtagaa
    13141 attactgctc tcacgcaagg acaaacagat gaaaatgaag gggcaggata taacccatgg
    13201 ggagaaggcg aacaaggcgg cttgttcgaa gttcaagtac ccaatgatga gagattaaaa
    13261 acggaggatc aagtgcaagt tgttgctgct aaaaaagtaa gaggtggcca agaagtgaca
    13321 gaactggact ctgccaaaac gacagtcgat gtgattcctc ctgaacctgc taatttaagt
    13381 accacattga tcaatgcagc ttctcgcacg atcacaggta caggtgaagc aggagccttg
    13441 gtttcattga aaaaaaatca agctgttttg tcagaaacaa ctattgatga tacaggggaa
    13501 tttgttcttt ctatcccaga aaatcaactt tttgcaggag atgtattaga aattgtatta
    13561 aacgaccaag caggagaagc tactgctaag ggtgtgcaga acaagccaag taccaacgat
    13621 gaaattggga atcacaatcc aagtgatacg ccagttgttt atcatgatgc cccgcctttt
    13681 gcacctgcta cgaagatcgt agtagaaggt ggtctctcat ttcttgcccc ttccaaactc
    13741 gattttggtc agatcaaagc aactggtttg acacaagaag catttggcca aatggatata
    13801 caggaaaaat tatggattta tgatcagcga gaaacgaaaa atccgtggat cttaacgata
    13861 aaattatctc agcctttcac aaatgatgca ggattttcta tgcaggatgt cctatatttc
    13921 aagaaaggag aggagtatca attagtgaat gaagaagaac tggaaattgt cagaggacaa
    13981 aattcaacga atgtgcaagc agattattca gaatatttaa ataacggtcg ggcgtttaaa
    14041 cttgtcttaa ccaaagaaaa tcagatagct ggtaattact cagcagagat aacatggaca
    14101 ttagcagatg cgccgacaga ataaagatca aggaggatga ttgatgaatc gaatatggaa
    14161 taaaagtcat cgaaacaact gctctttact tatgatcctt ctccttggat tcatcctttg
    14221 gcagacacct gtccatgcct ccgaattcaa ttttgcggta gattttgaat taccggctaa
    14281 tcagattgat aagaacaaaa catatataaa tgtaaggatg gagcctggga aaacacagga
    14341 aatcccctac acggtcttta accgaacaga taaatcagta acagttgaga cattgatcca
    14401 ctccgctacg acgaattcaa acggtgtgat cgaatatgga gaatcaaaaa gtaaatttga
    14461 tgaaacatta agttatgata tgaaaaactt agcaaaaaca gtgggagaaa taactgttcc
    14521 tcctcaaagt gcggtcactg agacactttc actgaaagcg ccagaaaaag agctggctgg
    14581 tgtggtcgca ggagggatca cgtttcaaga aaaaaaagag aacgtttcta acaaaagtac
    14641 agttggtgtc gtcaatcagt tttctcaagt gaaagcaatc ttgatttgaa atagcgaaaa
    14701 agagtcttcc ccagatttga aattaaaaaa agcttatgcg gatcaaagcg atggaagaaa
    14761 tgtgattaag gcgaatcttc aaaatccaaa tgcttcttat atgttcggct tgactacatc
    14821 ggttgaaatc tacaaaaaag aaacaaaaga gccttacctt tcaataaaaa aagaaaaaat
    14881 agatgtagca cccaatacaa attttgattt acttgtccca ttagaaggaa aaaaattagc
    14941 tccgggacat tatcaagcgg ttgtgaaggg tgcttggaaa gaaaaacgct ggaatctcca
    15001 taccgatttt gaaataactg atgatgcagc ggaccaattg aatgaaaagg atattgattt
    15061 aaaaaatcag ccagaaacat ttgactggcg atggctgata cttgttggag tcattcttat
    15121 tggcttattt gctggattat ttattttaaa agaaagaagg aaaaagaaat gaaaaaaaca
    15181 attgcattaa gtaccgttct agctggaagt ctattattat gtacacaaag tgtttttgct
    15241 gagaataaga gctcagccac tacagaagga acgatttctg tgaccaaacc agatgctgca
    15301 aatcctgaag acgttgttcc agttcctcct gttgatccaa atgatcctga tactcctttt
    15361 acacctgacg aaccaaatca gggaacatcc ggattactga cgattgacca agctccagat
    15421 cttgattttg gaacaatcaa gttaggtaca gcaaaaacag tatatgctgc attacaaagc
    15481 ggaacggaca gtactggagc acaaacacaa gtacagaatt tcgtacaagt aacggataaa
    15541 agcggaaatt atgccggttg gaaattatca gtcaaacgaa cagaatttat tggtacaaca
    15601 tctgatacga ataaactaac tggttcaaag gtgaaattca aaaacgccgt gattcgaaat
    15661 agcgcaacta atagcagtgg aatgccatct accgtttctg gaactggcgc tgaaggaatc
    15721 gaaatcccag cagatcaggc agtagatcta gtaactgcag gagcaaatga agggattggc
    15781 acatggattt attcattagg agagaatgtg gaacaaggga gacaatcagt ggaattattt
    15841 gttcctgtat ccaattatgc tgtagacaac tacgtttcaa cattcacatg gtcgttaaca
    15901 gatgctccta cagaagagga agcgtaaaat catatcaacg atttcaggga gtggagccga
    15961 ttcaaggccc actccctgaa atctgctatg cttacgtctc agctcttatt catcaactcg
    16021 aagcagtttt ttggaagtca tggaaacgtt cctttgtctt ttcaaagatc tttcgaccaa
    16081 cgagtaagga gtcaccgttg aaaaattcta acacataatg agcagaatca attttctgca
    16141 attgcgataa gttgatacag taagatttta aaagtaaaaa gtaagcatac ttttctaact
    16201 ctgtcttcaa ttgttttaaa gaagtattca ctaatttatc ttctgtagtt gttttgacca
    16261 taacttggtt tttcaaacct ttgatacttt caatatataa aatttcccga ggatacagga
    16321 cgagacttcc acctttttga tcaataaaga ttttatcgac atttacgatc tgctgttctt
    16381 tccaattgat ctgttctagc acttgacgaa tttgttcaga taaattcctg tcttttctga
    16441 tatatccaat cggaaggatt ttccgattga tgatctctac tgcaaaattc gtataagcgg
    16501 tgaaaaaaac aatattacta tgtgcatttt ttttacgtat ttgttcggcg acatcaatac
    16561 ctgtgagtga ttgcttcaag tcgatatcga taaagaaaac atcgtgatta tcaatagtgc
    16621 ccaactggat caactgattg atttgagaga tagaagtgag ctgcacgata ttctcgatat
    16681 gatgattgac caattctctg atgatccatt cgctgtgaaa ctggttatct tctatgataa
    16741 aatacttttt catatgcagg ctcctattaa aaaaatatgt gtacattttg aaacaaaaca
    16801 atggaggaat aagatggtcg caacagtgac actttatttg acctttactt tgattttaga
    16861 tttaggtatc tatttattct tttcgaaaca actgctagta aaaaacagct ttggttgtat
    16921 agctttgatg gtggtgacac ttttacttcc tatccctaaa gtgagctttc cttacctagt
    16981 tggcttatta ttgatgtttc ttttttattt tactacgaaa aacctgttta tcagctgttt
    17041 gatcagtaat cttttttttg ctatcactgg actcagcttt tttctggttt ttgatctgat
    17101 ggattattta ggaaagagac atcttggaac agcaagtttt tttgcactac ttatcgcatt
    17161 tattttctat ctattagtaa aaagtataga caaacagacg agatttctca acttgctttt
    17221 tacttatcca actgaacact attttggagg tatttgtatt ttattaagct ggggcgtgat
    17281 ttacttggta ttgggaagtg ttcacttccg ctcatctaat tatctgcttc tatctctttt
    17341 gtcgatagtc atcagttttt tttctttgtt tttttccatt atgttgatca gtttccaagt
    17401 aaaagaaaga caacatcagg aatcgcttca actgtatcaa ataaatgaag aatattacca
    17461 tcatctagag gaattcaaac atgattataa aagtctactg ttttcattaa aaattactct
    17521 tcaggaaaat aaaaaagaaa cgttggaatt tttagaaata ttagaagatc attctgcagc
    17581 tatttttgaa aaaccgcaaa tacaacagct aacaaatatt gtatccccag ctgtcagggg
    17641 tgtcttttta aaatttctag aaaaagcaga atcggagaaa atcccgatcc aagtagttat
    17701 tcctgtcgaa gtgaaaacaa tcccgattga tctggttgtt tttattcgct gtttatctat
    17761 ttttatttct aacgcattcg atcatcgtgt agccgatcag agtccgatta cgatcaatct
    17821 gctggaagaa aaagagggat cacgttttag tatagcgaac aaggcaaatc caacgaattt
    17881 gacgctatcg gagatggtca aaagaggaaa aacctctaaa aaaaatggcg ggttaggtct
    17941 atacagtgcg aaaaaaatgt tggatgttta cgaaaatgca gaactaaaaa tcgaattttc
    18001 aaaacagaat cactattttt ttgtagaaat gtatctggca tctagaaaga gcaaatcagc
    18061 ctactgactt actgtcaaag gataagtttg aggaaagtct acacggagat agagacaaca
    18121 acacagcata ttcaaaagaa acagaagcgt gatgaccgtt gtcacccttc tgtttctttt
    18181 gctatgtagt taaacaaagt agttgacact tccgctactt agtattagta cgaaagtata
    18241 cttcatgata ctaagtagat aagaagtgaa aagcggaacc cacgatcgtc aaaatgctaa
    18301 ctgctttgct tacaccagat aaagggaaga gcaatatcgc ttattttagg cgtggagaag
    18361 caagcaaatc cctacgatca caaaaaaaat gctgacccag cagttaagag agttagaaca
    18421 agatgaaagt atccatcgaa aagcctataa ccaagtcccg cctaaagtag agtactcgct
    18481 caccgaagaa ggaaaatcat taagagagat tctagtagcg ttgtctgttt gggaagaaga
    18541 acgtatcgaa aaacaaaaag cctcaaggga agaagacacg ctactgcata cacatggtgg
    18601 ctacctaaat tattgatttt ctatgacatt tattttaaat aaaagaaaac aggagttttt
    18661 ttaaaagcat catctatttt ttatgtaaaa aaatggtatg attaatcaag taagaagtga
    18721 ttggaagaag gcaagcaaat gaatagtatg gaaaaagtca accagtttag agatgaacgt
    18781 aattggcgac aatttcataa cgaaaaagat ttggctatct ctatttcttt ggaagcatct
    18841 gaattgttag aactttttca atggaaaacg ccggaagaag taaaagaaac acaattagaa
    18901 cgaatcaaag aagaactggc agatgtattg atctattcat acatgatggc agacaacctt
    18961 cacctggatc tagatgagat cattgaagaa aaattgatca aaaataattt gaaatatcct
    19021 gtagaaaaaa gcgcaggtgt acgaaaaaaa tacacagagt tgtagaaatg tgtcattcgg
    19081 ctgttggaaa gagcaaaaga aaaacagtaa aatgagtctg gagggagaga aaatgaaaaa
    19141 aaaaggaaaa gcaaaaagat ggctagtcaa tatcctcttg ttcctcttgt tattagtagg
    19201 actagctttg attttcaatg aacagatcaa ggattatttt gtcagagaga caggagataa
    19261 atatgctata gcgaatgtaa caaaagaaga tctgaaaaag aataacgata aagatgtcag
    19321 ttttgacttt gatgcggtag aaccaatgac gacagaaggg gtcatgaggt cgcaaatgag
    19381 aggaacagat ctaccagtga ttgctagtat cgcagtccct tcagtttcaa tcaatttacc
    19441 ggttttcaaa ggcttggata atacgtcact actttacgga gcaggaacat tatctccaga
    19501 tcaagaaatg ggaaaaggaa attatgcctt agcaagccat cgtgcaacga accctgaatt
    19561 gctgtttaca ccgctagaaa atttagagat gggtgccaag atctatttga cagatttaga
    19621 aaatgtgtat acatacaaaa cattttttaa agaaaaagtt gctcctacag atacccaatt
    19681 actgaatgaa gtcgagggaa aagaaattgt cacactaatt acttgtggcg acatggacgc
    19741 cgtcacgcga ctagttgtac aaggagaact ggaaagtgtt acatcgataa aagatgcgac
    19801 agatgatatg agatctgctt tcaatctaga gacaaaaaca ttttaggata ctgggacata
    19861 agtcgttaca gattaaaaac agccaaagga tatgaatcga taaggaaaaa ccaatttgct
    19921 gaaaaaaaga gaaacgcaaa gaggtgcatc tctgatttgg ttgttttcta gatagacttg
    19981 tgttccagtt tttttatttt ttatatgaaa aggcttgcat aaataaaaat tcgatggtat
    20041 gatgattttg aataagaaat ggattgttac tgtacggcag gcaaaaccag aattcagcta
    20101 agaaacagta ggcgaattct aggttttttt atttttcttg agagggcgaa gcaaaatgaa
    20161 aattgataaa attttgaata acaatgtcgt catttcgaaa aatggattcg gggaagaagt
    20221 cgtttgtatg ggaaaaggac tagcttttca aaaaaagatt ggggacgaga tttcgccaga
    20281 agcggtccaa aaagaatttg ttttaagaga ttcttttgct aaaaaccaat tccagcagct
    20341 gatggctgat attccggcag aggagatcga atgggtcaaa caagtagtcg aactggctga
    20401 agataaacta aaagttgaat tttcgcctaa tatttatctg acattgaccg atcatcttca
    20461 ttatgcgatc acacgagcaa aagaaaatat ccaactgcct aatccattat tatttgaaac
    20521 aaaaaaattt tatccgaaag aatatcaagc agcgaaagag gctcttttgt tcgtgaaaca
    20581 aaagacgggt gtggaacttt ctgaggatga agccggtttt attgcctttc attttgtgaa
    20641 tagccaacaa ggaaacgaag acatgcaagt gacgatgacc gctaccacaa tggtaagaga
    20701 tatactgagt atcatcagta aattcttcgg catcgtgttc gatgaagaat ctttgaatta
    20761 tcagcgtatc attactcatc tgcagttttt tgcccagcgt tatctcaaag gggaaggatc
    20821 tgacgagcaa gatgaatttc tctacgcatt agttcaagga aagtatccaa aagcgtttca
    20881 ttgtgtcgaa cggatcaatg agtatcttcg acaaacgcaa aaacaggaaa tggggatcgc
    20941 agagcagatt tatttaacga ttcatattca aagagtagta agtgagaaaa aaagcatcca
    21001 atcataagga ttgttactgt atggcaggca aaacctggat tgaaaaaata ggttcacgaa
    21061 tgtttgtgac tgtttttttc aatctgggtt tttatttttt aaataaaaaa gaaagaagga
    21121 aaaaacaatg gataatcaag cagttggtcg tcgtgtatgg gaagcggtag gaggacaaaa
    21181 gaatgtcaaa agtttagtac attgtgctac gcggttgcga ttcagattaa aagatgaatc
    21241 tttagcagat acgcaaaagt taaaagaaga cccggacgtg atccaagtcg tacaaagtgg
    21301 cggtcagtat caagtagtga ttggcagtaa tgtagcagat gtttatcaat cgatcgtgga
    21361 tgaggaaggt cttgcagttt cggaggatgg aaaagaagag tccaaaaata ttttgaatcg
    21421 tctgatcgat attatctcta gtatatttac accttttcta ggggcgatgg cagccgccgg
    21481 ggtattgaaa ggattcttat cattggcaac ggtgatgggc tggctgacag ctgatagcgg
    21541 tacttatcaa atattgtttg ctgcagcaga tggtgtcttt accttcttac ctgtcatgct
    21601 tgcttttaca gcagctaaaa agttcaaagc caaccagttt ttagcagtag caattgctat
    21661 ggcacttgtg tatccagcta tcacagcagt tgctggtgcg ggagaagcta tcagtttctt
    21721 tgggatccca gtgattcttt caccatctgg ttatacttct tctgtcatac caatcattct
    21781 agccgtatgg gttcaaagta aattagagcc attcgtaaaa aaagtgattc cgcaatttct
    21841 gcaaatgatt ttagttccgc ttgtcgtttt agtagtgatg gttccattaa catttcttgc
    21901 tttaggacca atcgggacgg ttgccggtaa tgctttaggc ggtctcttca actcgattta
    21961 tggatttagt ccaatcgtag caggtctaat catgggaagt ttatggcaag tattcgtgat
    22021 gttcggcatg cattggggat tcgtaccgat catgtttttg aatatcgaac aatatgggtt
    22081 tgatgtattg atgccaatgc ttcttccagc catcttggca caaggtgggg ctgctttagc
    22141 tgtagcattg cgaacaaaag atacgaaact tcgagcatta gggatttctt ctacagtgac
    22201 ttcgttattt ggaatcactg aaccaactgt gtatggtgtg acattacctt tgaagaaacc
    22261 attcattgcc gcttgtattt ctggaggtat tggtggagct attatcggat tttccggagt
    22321 aaaagctttc tccagcagtt tggtcagctt attgacgatt ccgactttca ttaatacggt
    22381 ggatggtgta gagtcaaatg tgacagtagc agtaatagct acaggaattg cttttgttct
    22441 tgcattcgta ggtacattga ttttaggatt tgacgaacaa acacaagaca atcaattaga
    22501 aaacaaacat gcgaatgcag gaaaaccgat tacttcagca cggcatacct taaaaagtcc
    22561 tttaacgggt aaagtactgc cattgagtga agtaccagac caagtttttt cttctggcgt
    22621 gatgggaaaa ggtatcgcta ttgatccaga agtaggagaa ctagttgcac ctgcagacgg
    22681 agagatcacg actatttttc caactggaca tgctgtaggg atcacaacga ctgacggagc
    22741 ggagattttg attcatatcg ggatggatac agtagaatta aatgggaatg gctttgagat
    22801 cttagtcaaa caaggggatc tcgtcaaagc aggagattta ttgattcgtt ttgacatcga
    22861 agcaattaga gcagctggct atagcgtaat cactccagtt gtcattacga atactgatgc
    22921 atttgccgat atacttgaat tagaccaaaa agagattatt gcaaacgaag atgttctggc
    22981 aattgtaaaa taaggaggaa atcaggatgc aatcacgttt ttcagaaaaa tttttatggg
    23041 gtggagcaac tgctgccaaa tcaattagaa ggggcttatg atcgagacgg aaaaggacta
    23101 tccgttgcag atgctatgcc tggaggtaag cagcgctttg cgattatcgg cagtgaagaa
    23161 tttgactgga cgattgatca agaaaaatat atttacccga atcatcgagg aatcgatcat
    23221 tacgatcgtt tcaaagaaga tcttgccttg tttgcggaaa tgggattcaa atgttatcgt
    23281 ttttcgattg cctggacacg aatttttcca aatggagacg aaggtactcc taacgaagcg
    23341 ggattagaat tttatgatca gctgattgat gagtgcttaa aatatgatat tgaacctgtg
    23401 atcacgattt ctcattacga aatgcctctt catttggcaa aagaatacgg tggctggaaa
    23461 aatcgaaaac tgatcgactt ctatgaacga tttgctcaga cagtattaga gagatacagc
    23521 agtaaagtca aatattggat gactttcaat gaaatcaatt cggcctttca ctttccagct
    23581 ttaagtcaag gattagtaaa aagcaatgga gcaggagagt accaaaatat tttccaagca
    23641 tggcataatc aattcgttgc aagtagcaaa gccgtaaaaa tcggccacga attgcgttcg
    23701 gatatccaaa tcggctgcat gattatctat gcaacaactt acagcatcga tgccaatcca
    23761 gtcaatcagg cagcaacaat gatccaaaac caagaattca attttttctg cacggatgtc
    23821 caagtacgtg gagaataccc tgcatacaca gcgcgtaccc ataaaaaata tggagtcgat
    23881 ccggaaaaat tagagcaaac ggaaaaagac ttccagttgc taaaagaata tccagtggac
    23941 tacatcggat ttagttatta tatgtctacg gcaatcaatg agactgatcc acaagcagca
    24001 acttcagaag ggaacttgct gggaggagta aaaaatccgt ttcttgaggc aagtgaatgg
    24061 ggctggcaga tcgatccaga agggctgaga atcgcattga atgaattata caatcgttac
    24121 cagaaaccat tattcattgt tgaaaacggt cttggagcca ttgatcatat ggaagaggat
    24181 ggcacgatta ttgatgatta tcggatcgat tatttacgtc gtcatatcga agcgatggct
    24241 aatgcagtag cagacggtgt agatttgatg ggctatacgc cttggggatg tatcgatctt
    24301 gtcagtgcat caacaggaga aatgagtaaa cgttacggct ttatctatgt ggatctagac
    24361 gatgaaggaa atgggacact gaatcgatca aagaaaaaat cttttgattg gtataaagaa
    24421 gtaattgcct caaatggcgt aaacttgtaa ttaaagcagc tgaatgaaaa agaacacact
    24481 tctcacatgt gatttgtgtt ctttttttat tttttatgtg ctattctttt agtaatagta
    24541 aagcaaaggg tgacgttatg aaaaggacac gtgtactata cttggaagtc gcagataaaa
    24601 ttaaagaaga tattttctca ggaaaatatc cagtcggttc gatgttgccg acagaaagtg
    24661 aattagaaga actgtttcaa gtcagcaaaa tcactgtccg aaaggcaatc gaactgctgg
    24721 catcagatga atatgtcgca aagaaaagtg gtcgtgggac gacggttcta agcaatcgtc
    24781 cttacaatcg attatccaaa gcggcaagct ttacgcaaat cttggagaac tcccgatatg
    24841 aagtccggaa agaaacgctg gatttgcaat tggtccatat aaagaaggaa gatcctttgt
    24901 tttccttttt cggtgagcaa gctgtttgtt ttaagcgctt atattatttg aacaatcagc
    24961 cttatattta ttttgttcat tatcttccac aagaaatgat gcaattcagc agagaagaat
    25021 ttggcaaaga atctttgtat cgtttgctga cgaaaaaagg atatactatt gactcatttt
    25081 ctgatgattt ctcggcagta tttttaacag aagaagaaca aggaattctg aaaacagacg
    25141 cacagatcgg catcaaacgg atacggaaat cgtctacgca aattggggaa atgattgaat
    25201 attcggaggc ggtctaccat acggagctgc atccttacca tattgattat gaaacataaa
    25261 taaaaaaaga ggttgtgaaa aagctgccct gcatcaagta ataagaacag ccaaacaaaa
    25321 atagcttctc atatttttcg tttggttagg cttattaccg cagatacacg gctgtttctg
    25381 gattaccatt tattctggat aagggggtgt ggcacgattt tcagaaggaa tctatgaacc
    25441 aatcactgta ttgattttag cttcgatggt aaccccattc ggcttgacga tcgcttattt
    25501 cttagggaaa atcattcgta aaaatatact caatcgtcaa gaaatcgata cattgaagac
    25561 cgcttttcct atggggatct gtcagatcac agagggatgt ttccctatcg tattgaacga
    25621 cttggcacgc aatgtcatag caacgggagt cggtgggaca gtaggtggcg gattaagcat
    25681 gttttggggc gcagatagcc gtattccggc atcgggaatg tttgcagtcg ctacgatgac
    25741 cagaccatgg gcattcattg gtgcattgtt agctggttct tttgtcacag gaataaccat
    25801 tttattattg aaaaaaccag tagatccgaa tgcagaaatc atccaagaag aaaaagagga
    25861 agaagacata tcttgggatg atttgacaat tagttaggag aggcagaatg gattacaaga
    25921 aaaaagtaca atagatgtac cttgagacag gaatggtatg aaagaatcag agttagaaag
    25981 aatcgaatat acagattttg gtttaggagc gatcgaaact gtccagagca tcggcaccct
    26041 ccttgaaatg acgatgaagg aaaaatgaga acctttcgtt gtcgaaaggg tctggtatac
    26101 cttatgtcga aggaaaggaa acaaaaaaac tttcctgtac tattccaaaa aggaaagaaa
    26161 gttattatac agtaaaaaaa gaaattattc ttcatgctag agatcagtac acgttcgagc
    26221 ccaatatcaa acactagttc caggcaggcg caacaggtgc ggttatcact gaattttctt
    26281 ccagcagtga cgattcaagt gatattttta caaatccagc tatttatcgt taacaaaaag
    26341 atttttagga gaagctgatg aacaaaaatg atcaaatttt gatcgtttag ttcgtcggct
    26401 tttttattta gagaaacatc atttatcagt gaaacataac acgagaacaa aatgattttt
    26461 tcgttaataa aagaaacaag tatttgttgt ttaaaataat acacgttatt ggcatatagg
    26521 acgaaatccc agtatcttct ataagtaagt catttcatat cgatgaaaac acgttattta
    26581 tttttgtttt atctataaaa tggctgaaaa atgatttttt tctaaatact atatatagtg
    26641 gcgtctatcc tatataccac taaaaaacta caaaatctag tcttttattt taaaaaaaca
    26701 cgtgtagaat gaagatagac ttttgagtta gggagtgttt ttttatgaaa cttactgttt
    26761 tttgcggttc ccgttttggt aataaagaaa gttacaaatt tattgcgcaa acgttaggaa
    26821 aatacatggc acaagaagaa atcgaactgg tttacggtgg ttcatactcc gggattatgg
    26881 gagtgattag taaaacagta ctggaaaata acggaaaagt cacaggtatc tatccgaatg
    26941 ggctttttga aactgaattg cctggaaaag atgttactac atttattcct acagaaacga
    27001 tcgatgaaag aaaggtatta ttatttgaaa agggagatgc tgtcctcgtt ttccctggag
    27061 gtctaggtac attggaggaa ttctcacaac ttctttcttg gatagcaatc ggtctgacgc
    27121 ccgacaagcc aatcggaata ttgaatattg gcggttatta cagcggcttg caacaattat
    27181 tagaaacttt tgcaaaagag gattttatgg ataaaaaatg gttggatcaa gtaatctttt
    27241 ccaacaatcc attggaactg gtcaatgaat tacgagcagt atctattaaa aatcagttat
    27301 tattgaagga ggcaaaataa tgagtcaaat atatttagca ggaccatttt tttcagagga
    27361 gcaaatcgat cgagtaagtc gtatcgaaaa agcgttggag gaaaacaaaa cagtcacaag
    27421 tttctactct ccacgtcatc accaagaaag caattatgaa ctattcagcg ctggatgggc
    27481 gcaggaagta tacgaaaaag atatggaaga actgacaaat gcagaatttg ttgttgctat
    27541 ccttgacttt gagcatcaaa caatcgatcc aggaacggct tacgaattag gtgtggcaac
    27601 tatgttgaaa aaaccgatga tcattgtaca agaagagacg gtacctacga acttgatgat
    27661 tacacaaagt ctccatacgt atttaaaatc agaccaagct gtacgtgaat atgattttga
    27721 aacattgcct gttgaaactt acgtaggcga gtacttatag aagaggaagg gttgtatgtc
    27781 agtgattttg atttcaggaa cgattggtgc aggtaagagc agtttgacgg atatgcttgc
    27841 aaaagagatt gattctaaac cattttatga aaatgtcgaa gataacgaag tattgccgct
    27901 cttttattca aatcctgagc agtacgcttt tttattgcaa atcttctttt tgaacaagcg
    27961 atttttagca atgaaaaatg ctttagtgaa cgatgacaat gtgctagacc gatccatcta
    28021 tgaagatagc ctgttgttcc atctaaacgc tgatttaggt cgggtaacgg atatcgaagt
    28081 acagcagtac gatagcttat tagatacgat gctaaacgag ttagatgatg ttgctccaaa
    28141 aaaacggcct gatttgatgg tccatatcaa agtttctttg gacacgatgc tagaacgtat
    28201 caaaaaacgt gggcgtgact atgaacagct agaaagcgat gagacgttat atacctatta
    28261 tgaaacgttg aacacccgct ataatcaatg gtatgaagat tttgatgtat gtcctaaaat
    28321 acaagtggat ggggataaat tcgattttgt agaaaatcca gaagatgcaa aaaaggtgat
    28381 ccgtcagatc caagaaaaat tagctgaatt ggaaggaaag cctgctggaa aatattttac
    28441 ggataaccag catgcaaaag gtcaagaatt aggctaaaaa taaaaaacca aacgacggga
    28501 aagaaaaatt actacgtgat gtagaataag cctttctatc gtttggttat tttatattca
    28561 aatatttgaa ataaataaat ctttactctt tgatttgtaa actgaaatga tccactgttt
    28621 gttttactga tgaagcttga tcaaaggaag aagctgagtt tgaagttcat aaaaatcagc
    28681 catagtgagt tttgcttgga tttgtttatc ttcttctact ttttacataa ttttgaacag
    28741 gataagattc aaatgtttta aatgaatctt tgactagttt gtgacagttg tcgcatatgc
    28801 tcaagcttcc ttggtcatta aattctgtat ggatattttt gttgttatta catgtacaat
    28861 aatcaggact atcgatcatc catttcgtct cctttacaat catcttgctt ctttactata
    28921 gcagaaaaga aggggagaaa caaaagaaga tccaacagac ggcactatag actgtagatg
    28981 ttgccatcct catccttcaa acggtaatta ccgatttttg taaatatgac tgttgtttct
    29041 tttttatttt cttttcctga aataatatat tcgtcatctt tggtagtgat cttgatatct
    29101 gtcagcactg ttttttcttc agatgattca gaagaatcag ttccttgtgt ctgatacgtg
    29161 cgtgtccatt ccattttttt atctgtaaat gtcaactgtc ccgcggataa atccatatct
    29221 ttacgtactt ttagcgtata tacactgttg gtggtatctg ctttttccgc ttttaagaca
    29281 gaagaatcgg ttgtttcgtc cgaggattgg tcagagccgc caaacaaatc acagccagtc
    29341 aatgtcaaaa gtcccgcaga caaaataaat agtacgatta atttcttcaa aatacttccc
    29401 ccatttgata ggataaagaa attataccat tggaatgtaa aacttcaaac ggatggaaga
    29461 gaaaaacgca caaaacaccc acaaatagag taaattttag aataaaattt taaaaaggca
    29521 aaaaatccgc cactttcaga aagcgtttta aaaaagtgtt gcaatctttt aaaaaaatgc
    29581 tatattaaaa atgaaccggt tcaaatactt gtttaagtat aattaatagt gagaggaagt
    29641 tgctctactg aagactttag ttatatggat attgatgaca agcaacacta tgcaaagaat
    29701 gcggtcatca taatcgaaca acaagaaaga gaaatcaaat gtaactggca ggagtgagaa
    29761 tgtatggcat cgatccgtga tgtggctaag atggctggtg tttcagtggg aacagtttcc
    29821 cgttacttga atggtcagca actgaaggaa aagaacatga aaaaaatagc agaagcgatt
    29881 tcagcgttgg actataaaga aaacatcatt gctaaagggt tgaagaataa tcgaagcttc
    29941 tctattggac ttttgataaa cgggatttcc agccgttttg ggtcggaagt tgtctcaggg
    30001 atcgaaagag tagctgaaga aaatgggtat agcttattat taagcgggtt tgccgatcag
    30061 ccagaaaaaa ttgaacaaaa aattgaatac ctgatgaagc atgtgatcga tgggctgatt
    30121 gtcttcttat ccgaagaaga gtggtctgga tttgaaatgc tcacaaaaat gagtattcca
    30181 gttatcgcat tgaattgtcc agaaggacca gcaggtattg atacaatatt agtgaatgat
    30241 cgagaaagtg tagcaaaagt gatcgctcat catggtgaac aaggacataa aaaaatcgga
    30301 tttattgctg ccactcaaac agactacgta gcaagagaac gtttagcagg agccaaagaa
    30361 gcggtccaaa atgatccatc gatccaatta gaggtcttta caggagacta ttctagaaaa
    30421 agcggctatt acggggcaaa agcattatta gaaaaagggg caactgctat ttttgtcagc
    30481 aattacaata tgtcgattgg tgccatcgaa tgtttcaatc aagaaggtgt cagaatcggg
    30541 aaggatattg cgttcagtca ctatgattat ttagataaaa acgatctgag tatccttccg
    30601 aaaatcacga tcacgccacc aacagataaa atcggggaac gagcagcaga acagctgtta
    30661 aaacgaatca aaaaagaaga ggaagaaaat gaaaaagtga ttgtgatgga aaatatcata
    30721 catggaatca actaaaacga actaaaaaag aaaaagaggt gctttgatga aaaaatatca
    30781 atttccagca aatttttggt ggggttctgc agcaagtggc ccacaaacag aaggaaggat
    30841 ttcaggcgat ggaaaaggag agaacatttt tgattattgg tacaaaaaag aaccgcaaaa
    30901 gttctttgat caagtaggac ctgaaaaaac ctcgcaagtt tatacgaagt atcaagagga
    30961 tgtacagttg atgaaacaga ctgggcataa ttcatttcga acatctatcc aatggagccg
    31021 tttgatacct gatgaatctg gaacaatcaa cgaagaagcc gttcgtttct atcatcgtta
    31081 ttttgatgag ttgattgaaa acggtatcga accctttgtg aatctgtatc atttcgatat
    31141 gccgatgtat ctgcaagaaa aaggcggatg gttgaaccga gaaacggttg aagcctatga
    31201 aaaatatgcc aaaagttgtt ttgaattgtt tggttcgaaa gtaaaaaaat ggtttactca
    31261 taatgaacca atcgttccag tcgaagcagg ttatttatat cgatggcatt atccagaaga
    31321 gtcagatatg aaaaaagcga tccaagttgg ctatcatgaa gcgttggctt cagcgttagc
    31381 catcaaatct taccatgaaa tggagcaagg tggtgttatt ggtatcatct tgaatctgac
    31441 accaagctat ccaagagacg aatcaaaccc tgaagatgtt aaagcagcgc aaatagcaga
    31501 tgcgttcttc aatcgttcat tccttgatcc agctgtaaaa ggcgagtttc caagtgaatt
    31561 gattgagata gtaaaagaac ttgatctcat gcctgtgatc catgcagagg atttgacggt
    31621 tatcaaagaa aatacagtag atcttttagg tatcaattat taccagccac gccgggtaaa
    31681 agcaaaagaa acaccaatcg atactaaaaa cggtccaatg cctgaggatt atttcgacaa
    31741 ttatgaaatg cctggtcgta aaatgaatcc ttatcgcgga tgggagatct atgaacaagg
    31801 aatctatgat attttgatca atgtacgaga tcattatgga aatatcgact gctttatttc
    31861 tgaaaacggt atgggtgtag aaaacgaagc acgatttatc aaagaagatg gcatgatcca
    31921 cgatgattat cggatcgaat ttgtccaatc tcatctgaaa tatgtccatc aagccattca
    31981 agaaggtgcc aattgtttag gctatcatat gtggacatgt atggataact ggtcgtggac
    32041 caatgcatat aagaatcgat atggctttat ttcagtagac ttagcacaag atggaaaaag
    32101 aacaatcaaa aaatcgggtc attggttcaa agacgtatca gataaaaacg gatttgaggc
    32161 ttaagaaaga atagttgaga gcaaatgcgt gaaattctcc attcttacca ttttgtgaaa
    32221 aagaagttca attatttacg atctttttca acttttttgt c
//
LOCUS       DAYYCY010000014        30228 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000014 DAYYCY010000000
VERSION     DAYYCY010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30228
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.014"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            2..370
                     /locus_tag="ACHAZB_000523"
     CDS             2..370
                     /locus_tag="ACHAZB_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFM8613223.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            550..1491
                     /locus_tag="ACHAZB_000524"
     CDS             550..1491
                     /locus_tag="ACHAZB_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFM8613224.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1560..2291
                     /locus_tag="ACHAZB_000525"
     CDS             1560..2291
                     /locus_tag="ACHAZB_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM8613225.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2435..4615
                     /locus_tag="ACHAZB_000526"
     CDS             2435..4615
                     /locus_tag="ACHAZB_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFM8613226.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4635..5117
                     /locus_tag="ACHAZB_000527"
     CDS             4635..5117
                     /locus_tag="ACHAZB_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HFM8613227.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5133..5894
                     /locus_tag="ACHAZB_000528"
     CDS             5133..5894
                     /locus_tag="ACHAZB_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFM8613228.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6025..7359
                     /gene="murC"
                     /locus_tag="ACHAZB_000529"
     CDS             6025..7359
                     /gene="murC"
                     /locus_tag="ACHAZB_000529"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFM8613229.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            7666..8151
                     /locus_tag="ACHAZB_000530"
     CDS             7666..8151
                     /locus_tag="ACHAZB_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HFM8613230.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8144..8827
                     /locus_tag="ACHAZB_000531"
     CDS             8144..8827
                     /locus_tag="ACHAZB_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFM8613231.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGIILAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            8953..11598
                     /gene="polA"
                     /locus_tag="ACHAZB_000532"
     CDS             8953..11598
                     /gene="polA"
                     /locus_tag="ACHAZB_000532"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFM8613232.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            11643..12479
                     /gene="mutM"
                     /locus_tag="ACHAZB_000533"
     CDS             11643..12479
                     /gene="mutM"
                     /locus_tag="ACHAZB_000533"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HFM8613233.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12443..13072
                     /gene="coaE"
                     /locus_tag="ACHAZB_000534"
     CDS             12443..13072
                     /gene="coaE"
                     /locus_tag="ACHAZB_000534"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFM8613234.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEILVGQIENWLFANRFL"
     gene            13124..13456
                     /locus_tag="ACHAZB_000535"
     CDS             13124..13456
                     /locus_tag="ACHAZB_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFM8613235.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            13768..14259
                     /gene="nrdR"
                     /locus_tag="ACHAZB_000536"
     CDS             13768..14259
                     /gene="nrdR"
                     /locus_tag="ACHAZB_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFM8613236.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14282..15655
                     /locus_tag="ACHAZB_000537"
     CDS             14282..15655
                     /locus_tag="ACHAZB_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFM8613237.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            15655..16581
                     /gene="dnaI"
                     /locus_tag="ACHAZB_000538"
     CDS             15655..16581
                     /gene="dnaI"
                     /locus_tag="ACHAZB_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HFM8613238.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            16710..17150
                     /locus_tag="ACHAZB_000539"
     CDS             16710..17150
                     /locus_tag="ACHAZB_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFM8613239.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            17294..17995
                     /locus_tag="ACHAZB_000540"
     CDS             17294..17995
                     /locus_tag="ACHAZB_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase TrmK"
                     /protein_id="HFM8613240.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            17992..19113
                     /locus_tag="ACHAZB_000541"
     CDS             17992..19113
                     /locus_tag="ACHAZB_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFM8613241.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            19128..20360
                     /gene="pepT"
                     /locus_tag="ACHAZB_000542"
     CDS             19128..20360
                     /gene="pepT"
                     /locus_tag="ACHAZB_000542"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFM8613242.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            20492..21400
                     /locus_tag="ACHAZB_000543"
     CDS             20492..21400
                     /locus_tag="ACHAZB_000543"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFM8613243.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            21435..21707
                     /locus_tag="ACHAZB_000544"
     CDS             21435..21707
                     /locus_tag="ACHAZB_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HFM8613244.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            21718..22764
                     /locus_tag="ACHAZB_000545"
     CDS             21718..22764
                     /locus_tag="ACHAZB_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HFM8613245.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            23015..25624
                     /gene="clpB"
                     /locus_tag="ACHAZB_000546"
     CDS             23015..25624
                     /gene="clpB"
                     /locus_tag="ACHAZB_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFM8613246.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(25775..26461)
                     /locus_tag="ACHAZB_000547"
     CDS             complement(25775..26461)
                     /locus_tag="ACHAZB_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8613247.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(26439..26933)
                     /locus_tag="ACHAZB_000548"
     CDS             complement(26439..26933)
                     /locus_tag="ACHAZB_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFM8613248.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(26953..28173)
                     /locus_tag="ACHAZB_000549"
     CDS             complement(26953..28173)
                     /locus_tag="ACHAZB_000549"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8613249.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            28320..30155
                     /gene="lepA"
                     /locus_tag="ACHAZB_000550"
     CDS             28320..30155
                     /gene="lepA"
                     /locus_tag="ACHAZB_000550"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFM8613250.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
BASE COUNT        10133 a         5387 c         6523 g         8185 t
ORIGIN      
        1 gatgtttacg aatgaaataa aaatcatgct ttatgtggac gatgtagaaa aaaatgcaac
       61 gttttggaga gcagttggct ttgcggaaaa agatcgtcaa gaaatggatg ggacgcttat
      121 aattgaagta ggagttgagg acagtcaaac ctcatttctg ttatatgatc gagcatttgt
      181 tgagtcccat tcacctggca cagctactag tgccccatct ttgatgtttt acagtaaaga
      241 tatctttgga ctatataaaa aaatgcagac gcaatcagtc acattaggtg atatggtaca
      301 gcttggagaa gaatatgtct ttaactttgc tgatcccgat ggcaactact ttgcggttac
      361 aggtaaataa tagaaataaa gactttgttt ttctgtgcga aacaggaaag cgaagtcttt
      421 ttaattcaaa taataatatt atgtaaacaa aaaactttcc atgcgtaatt atacacaagc
      481 aaataaatcg tcaaatgcga acgccttctt tatactgttt aggaacgaaa agaaggaagg
      541 aagaaataca tggattatca agtactgctt tattacaatt atacaaaaat cgaagaacca
      601 gaggaatttg ccaaaaatca tttagccttt tgcaaatctt tggatcttaa aggtcgtatt
      661 ctagtagctg aagaaggaat aaatggtact gtgtctggaa ccgttgaaaa cacgactgcc
      721 tatatgaaag caatgaaagc tgatcctcga tttgctgata cgatgttcaa gatcgatcca
      781 agcgaaggac atgcctttaa gaagatgttt gtaagatacc gtccagagct tgtaagttta
      841 aagctagaag aggacattga tccaaaccaa gtgacaggtg cctatctttc accgcaagaa
      901 tttaaagaag ctattttaga tgaacagaca gtggtgattg atgcacggaa tgattatgaa
      961 tatgacttgg gacattttcg aggggctgta cgcccagata tacgcagttt tcgagagctt
     1021 ccagactgga tccgcgagca caaagaagaa tttatggaaa aacgggtagt gacctactgt
     1081 actggtggca ttcgttgcga gaagttttct ggttggcttg tccgtgaagg attcaaagat
     1141 gtcggacagc tgcatggcgg tattgctact tatggtaagg atcctgaagt ccaaggagaa
     1201 ttatgggacg gcaagatgta tgtgtttgat gaacggatcg ctgtcgatat caaccaagta
     1261 gataaacaaa tcgtcggtcg cgactggttt gacggaacac catgtgaacg atatatcaat
     1321 tgtagcaatc ctgagtgcaa tcgtcagatt cttgcttcca ttgaaaatga agaaaaacat
     1381 ttaggagcat gtagcataga gtgtgcgaaa catccgcgca atcgctatgt tcaagaccat
     1441 ggaatctctc cagaagaagc agaactgcgg tttacagctt taattaaata gaaaacaata
     1501 gttttgattg tcgaccattt ccaatatgcg ctatactagc gttgaggatg gtgatccaca
     1561 tgaaatcatt gatttatgct attggggatg tccatggcaa acatgagctt tttgaaaaac
     1621 ttattcacta ttatgatcca aagatccatc agcttgtttt attaggggat ctcaatgatc
     1681 gaggtcctcg tacaaaagaa tgttttttat taggaaagca attagtcgaa gaaacaggag
     1741 ccatttacct ccggggaaac catgaagaat attttttgca atttctgcaa tcaccggagg
     1801 actggtatgc gccttatatg agaaatggtg gaaaagaaac gtttgaaagc ctgattcacc
     1861 caggggcatc ggaggaatac tccccaactg aaatctcgat gatgatccaa tcgagatatt
     1921 cagaactgat taaattttta gtccagcgcc ccttgtatta tgaatgggaa aattatcttt
     1981 ttgtccatgc cggtgtggat ttgacaaaga aagactggcg taagacaagt ctaagagact
     2041 ttatttggat aagggaacca tttcatgaag ggaaaaacaa tacaggtaag acaattgtat
     2101 ttggacatac gattacacca atgctccatg gagatatgca gacaacagac ctctggattc
     2161 aggatcataa aatcggaatg gacggtgggg ctgtattcgg agggtcattg catggggttg
     2221 tttttgacaa ggaaggtatt cttcaagata ttgaatatca gaacaacgaa ggaccttggc
     2281 atggaatctg actaatggaa agaatgaagg agctgtgact ttgttgcggc tctttttatt
     2341 tttatagaga atattgtttc tttcagtagt ttaacccagg ataaaaagta tgatagaata
     2401 aagaagcaat ttttagcaga aaagaggcat tttaatgacg gaaaatttga accagacgat
     2461 cattcagctt gtacaaaaag aactttcaga ctatcgtcct aagcagttga ctactgtact
     2521 taatttgtta aatgaaggaa acactgttcc ttttatcgcg cgttaccgaa aagaaatgac
     2581 tggaagtctt gatgaagtcc agattcgtga aatcgaagag cggtatgcct atcttgaaaa
     2641 cttagaaaaa cgtaaaactg aagtgattcg gctaatcgat gaacaaggga aactgactcc
     2701 cgagttagaa gcagaaatca cccaagccgt gaaaatgcaa caagtggaag atttgtatcg
     2761 tccatacaaa caaaaacgac ggacaaaagc aacgattgct aaagaaaaag gacttgaacc
     2821 actcgcgaag tggctgatgc agcttacaga tggagaggtg caatctgaag cagaaaaata
     2881 tattgacaaa gaaaaagaag tctcttctgc cgaagaagcg ttgcacggtg ctcatgagat
     2941 tattgctgaa caagtcagcg ataatgcaaa atttcggact tggattcggt cttatacgta
     3001 taacaaaggt atgtatgtca gcaatgtcaa ggatgagcaa gcagatgaaa aaggcgtata
     3061 tgaaatgtat tatgactttg cagaacccgt ccataaaatg gtttctcatc gaatattagc
     3121 tactaatcgt ggagaaaaag aagatattct gaaagtattc tttcaagtag atgaagcagc
     3181 aattttagct tacttggatc gccaaatagt caaaaatcct gcctctcctt cttcttcatt
     3241 cgtcagagaa gcctatcagg atagttataa acgctttatt cagccagcaa tcgagcgaga
     3301 actgcgcaat gaattgactg aaaaggcaga tgagcaagca atcgctattt tcggcgaaaa
     3361 cctccgtaat cttttgttgc aaccgccact aaaaggtaaa gttgtcctgg gatttgatcc
     3421 ggcttatcgt actggatgta aactagcagt cgtagatgcg acagggaaag ttttagcaat
     3481 cgaagttatt tatcctcata aacctgcagc gcaagcaaaa agagaagctg ctggaccagc
     3541 ttttatccaa ttaattaata aatatcaagt agatatggta gctatcggaa atggtacagc
     3601 cagtcgggaa tctgaacttt ttgtggctga gcagttgaaa tccgccgacc ataaaaccta
     3661 ttacgctatc gtcaacgaag caggcgcttc tgtttactcc gctagcgaaa ttgcacgaaa
     3721 agaatttccg catctacaag ttgaagaaag aagtgctgtt tctattgccc gtcgcctgca
     3781 agatccttta gctgaattag tcaaaatcga tcctaaagct gtcggagtag gccagtacca
     3841 acatgacgtt tctcaaaaac gtttggctga acaattggat tttgttgtgg aaacagcggt
     3901 taaccaagta ggtgtggatg tgaatacagc aagcccgcag ctcttgcagc atatatcagg
     3961 attgaataaa acaactgccc aaaacattgt catttatcgt gaagaaaacg gagaattcac
     4021 cgcacgaact caattgaaaa aagtgccacg cttaggacca aaagcatatg aacaagcaat
     4081 cggattcttg cgagtccctg gtgggaaaaa cattttggac aacacaggta tccatcccga
     4141 aagttattct attgcaaaag aaatattaac ttcagtccaa ctttcagaaa aagagcttgg
     4201 tacagaagaa gcagtagaaa aattgacacg attatccgta gagaaactgg ctgaaagcct
     4261 atcagtcgga gaagaaacgt tagccgatat tttagctggg ctgacacaac ctggacgaga
     4321 tatgcgtgat gagatgccag cgcccttatt acgtacagat gtattgagta tggaagactt
     4381 gaaaccaggg atggaattga ctggaacggt ccgtaatgtc attgacttcg gcgcatttgt
     4441 tgatatcggt gtgaagcaag acggacttgt ccatatatcg aaactcagca aaaaattcgt
     4501 caagcatcct acagatgtcg tgtcagtagg agatatcgta acagtttgga tagaacaagt
     4561 cgatacgaaa aaaggcagaa tcagtttgac gatgctttcc ccttatgaag agtaacaatg
     4621 aggagtaaca aaaattgaca caagaagaac tgcagcagtt agtagaagag atatcattaa
     4681 aaagcttcca gcttcctttc aagcaccaag caatgttcaa tccacgctta cgtacaactg
     4741 gcgggcgcta tcatttaaac gatcatcatt tagattttaa tgcgaagcta tttgaagagt
     4801 cagacgaaga gacgattgca ggaatcatca aacatgaact ttgccattat catcttcatc
     4861 tagctggaag agggtatcag cataaagata tggatttcaa gtatcttttg aaaaaaacag
     4921 gagggttgcg ctatgcacca gccttgacga agaaacagat aaagatggag tattatcgat
     4981 gccaagattg ttctgccacg atctgccgca agagaagaat cgatgtacgc aaatatcgtt
     5041 gtggaagatg tcatggagaa atcaaatgga ttgagacaaa aagagaagac tttaatgaag
     5101 cacaaaaaat cagatgaaag gatagaaggc ctttggaaaa aagtgagcag aggaatctaa
     5161 aagactggaa tcagctatta aattttttag ccgcacctga agagggaaat tttgaggaac
     5221 tgacggcacc tacacttatt ttagctggaa actgtcttcc tatcttagct gataaagcag
     5281 cacaaatgta tttaaatggc caagtagatc aattgttttt ggtcggaggg gtggggcatg
     5341 cgactagaat cctttatgag aattttgaaa agcaaggatt tcattttgaa gatggcatga
     5401 gtgaaagtga aatctgtcgt cagtatctaa aagaagtcta tgatttgccc gataaggctt
     5461 ttctgataga atcgaagtct acaaattctg gagagaacgc cactttttca ctggagattc
     5521 ttcattcctt aggagctgta ccagaaaaaa tcttattgat gaatgatccg acactgcaac
     5581 gcaggactcg agcaactttt gagaaagtat ggcaaaatga acaaactatt ttcgcgaatg
     5641 ctgtgccatt tgtgccggaa atccttcact tttctgaaga gatcatcttt acagcaaaag
     5701 aactgaatca tcaatggcct aaggaatatt tccatgcgct agtattagga gaaatggaac
     5761 gtctccatga cgatgagaac ggctatggtc cgaaaggaaa agattttatt cctcacattg
     5821 atatatcaga ggaagtttgg tcttcttaca ccagaattaa agagtcaata aaaacggatt
     5881 tttctcgtac ttgaccattt tagcaagcga atcctccttt ccaaataaat ttggttgaaa
     5941 ttttcaatcg tttataagat aatgaggagc gtgttgaatg taatagctaa aagaatggac
     6001 gctagataag agaagaggat tcaaatggaa aatcaaaata aattgtatca ttttgttggt
     6061 atcaaaggtt caggaatgag ctccttggct cttgttttac atgaacaagg tctaaatgtc
     6121 caaggatcag acattgaaaa atatttcttt acacaaagag atttagaaaa agcaaatatc
     6181 acgattctgc catttaacgc agataatgta aaaccaggga tgacgatcat tgcaggaaat
     6241 gcatttcctg attcacatga agaaatccag cgagcaaaag aattaggttt ggaagtgatt
     6301 cgctatcacg actttattgg tcattttatc caaaattata cgagtatcgc tgtaacaggc
     6361 tctcacggaa aaacaagtac gactggactg ctctctcatg tattgagtgg tgtccgtcca
     6421 acaagttatc taattggaga tggaacagga catggtgatc cgcaagcgga attcttttca
     6481 tttgaggcct gtgaatatcg ccgtcatttc cttgcctatt caccagacta tgcaatcatg
     6541 accaatatcg attttgatca tccggattat tatacaagta tcgacgatgt atttacagct
     6601 ttccaaacaa tggctggaca agtgaaaaaa gctatttttg cgtatggtga cgatgcttat
     6661 ttacgcaaat taaaagcaaa tgtgccgatt tattattatg gtgtcacaga aaacgatgat
     6721 atccaagcac gaaacatcga acgcacaact tctggttctg cttttgatgt ctatcatggg
     6781 gatgaatttg ttgggcactt cactgtacca gcattcggta aacacaatat cttaaatgct
     6841 ttaggtgtta ttgcagtagc ttatttcgaa aaactggatc tgaaagaagt tgcagaagaa
     6901 atgctgactt tcccaggagt aaaacgtcga ttcagtgaaa agatcgtagc agacatgacc
     6961 gtagtcgatg attatgcgca tcaccctgca gagatcaaag caacgattga tggcgcacgc
     7021 caaaaatatc cagataaaga aataattgca gttttccaac cccatacgtt tacgcgtacg
     7081 attgcattga tggatgaatt tgcagaagca cttgatttag ctgataaagt ttacttatgc
     7141 gatatttttg gttcggctcg ggaagaacaa ggaaacgtta agattgaaga ccttggagcg
     7201 aaaatcaaaa aaggtggaga agtcatcaaa gaaaataatg tatctccatt gttagattat
     7261 catgatgcag tagtaatctt catgggtgct ggagatgttc aaaaatttga acaagcgtat
     7321 gaaaaacttt taagcagcac gacgaaaaat gtactgtaat acaattactg cagtgtcctt
     7381 ataaaaatag aaagaggggg agtggcaaaa atcttgccaa gccccctttt ttgcaactta
     7441 tgataaacgg tgttcaaaag cttgcatctg cggtaataag cccaatcaaa tcaaaaacat
     7501 gagaagctgt tttttcttgg ctgttcttat tatttgatgc aggacagctt tttcacgacc
     7561 tctgtaaact ataggaatct gttaaatcaa gggagcagaa cttgcctttt acgctacttt
     7621 tggtacaata atgtatgatt aaaaataaac tggagggttt ctatcttgaa cataggtaag
     7681 ccgagaaaac taggaaaaac aataaatgaa atcgaggaag gcgactcgtt atccttaaca
     7741 gaatctattg aagataacca gcttttgctg tatttgggat tgacgaatga tgcaaatcct
     7801 ttgtatatcc aacatgactt tgcacaaaaa acagaatatc aacagccaat cgttccatca
     7861 gtgatgctga tggggatcat cacaagtgca atttcaaagc atcttcctgg accaggatca
     7921 catgttgtca atttttctgt caattttatt gaaccggttt tccattatga aacactgact
     7981 tttcagtttg aggtcataaa agtagataag atgaaggatg tggtgacgat ttctgtagaa
     8041 gcggagaata cgaacgaaga tcgtgtgttg gatgctgtcg ttatggtcca accgccacaa
     8101 gttcctgaat tggaaatagc aatcgaaagt gaaggtgaat tgaatgaata atccaagtac
     8161 atcaatgtca ggtatcaatc gtttttatgc aaaagtttat ggatttttag gtatgggact
     8221 tgcccttagt gcagtcactt cttacttggc tctttgggta tttcctttgc aattagcaac
     8281 ttttatcaat aattttccac taggctttgt gggtctgtgg atcgttgaac tgattttagt
     8341 aatggttcta ggcgtaaaag cacaaaagaa tccgagctta gcaatcggtg gatttattgc
     8401 ctactctctt ttgaatggca taatattagc ggttactctt gcatactacg atattggaac
     8461 agtaacgcaa gctttcttga cagctacttt gatgttcatg gggatgtctg ttgtcggagt
     8521 aacaacaaaa agagatctta gcggggttgg aagagcagga caggcagctt taatcggagt
     8581 gatcattgca ctagtcttga acatcttcat tttgagaagt agcgtagttg agctgttcat
     8641 ctccctactg ttagtcgtca tctttgcagg gattacagct tatgataacc aaagcatccg
     8701 taatatctat gtccagacaa acggaacagc acatagtgga attgctgtat tcatggcatt
     8761 gaagctgtat ttggacttta tcaacttgtt cttggcgttt ctgagaatct ttggacggaa
     8821 caactaaaaa agaaaaaaat tcatcagttg gggctatacc gaattatttg gtatggtccc
     8881 gctttttctt ttcgcattcc gcaggattct ttgttagaat gaataagaac tttacagtaa
     8941 ggagttaaga agatgacaaa aaataaatta ttactcgttg atgggaacag tgtggctttt
     9001 cgcgcattct ttgcgcttca caattctctt gaacgtttca aaaatagaaa cggtcttcat
     9061 acaaatgcaa tctatgcatt caatacgatg tttgaaaatg ttatgcaaaa agagcaaccg
     9121 actcatgttc tggttgcgtt cgatgcagga aaaacgactt ttcggacaga aatgtatgcg
     9181 gagtacaaag gtggaagatc caaaacacct ggcgaattca aagaacaaat gccatatata
     9241 cgtgatttgt tgacaggact aggggtacag tattacgaat tgcccaatta tgaagcagat
     9301 gatattatcg gtactttggc agaaaaagta gacaaagatc aatttgatgt cgtcgtgctt
     9361 tcaggtgatc gggatttgac tcaattagct agtaaagaag taaaagtgga tatcactgta
     9421 aaaggtgtca gcgatatcga aagctataca ccagaacatg tagcggaaaa atatgatggg
     9481 ttgacgccaa aacagattat tgacatgaag ggactagccg gagatgcctc tgacaatatc
     9541 cccggtgtga ctaaaatcgg tgaaaaaaca gctatcaagc tattgaaaca atatggctca
     9601 gtcgaaggaa tttatgaaca tatcgatgaa atgaagcaaa gcaaaatgaa agaaaatctg
     9661 ataaatgaca aagagcaggc tttcttatcg aaaaaattgg cgaccatcaa tacttcagcg
     9721 cctgtggatg tctctatcga ctcattgaag tatgaaggga aaaatctcga taaactcgtt
     9781 ccgttttata aagaaatgga tttcaatcag ttcctttcta aattgaatat cgtagaagaa
     9841 gaagtaaaaa tggatgatat tttgtttgaa gtcgttcatg aattcaaaga ggaaatgttt
     9901 actacggata tggcgttgta cgtggaaatg atgggcgaca attatcatac agaggaaatc
     9961 gtcggagtag cgtgggggac agaaaagaaa atttatgtca cgaacgatct atctgtattc
    10021 gaaaaccgag cgttccattc ttggatcact gatccgactc ggctgaaaaa agtatatgat
    10081 gcgaaacgaa cttatgttgc tttaaaccgc tatactggaa aaacgaaagg aatcgatttt
    10141 gatgtcttgc taggtgcata ccttctcgat acgaatgaaa aaagcacaga tatcgaagga
    10201 atagctgctc attacggata caacgatatc caatcagatg aagctgtata cggcaaaggg
    10261 gcgaaaaaag gtttaccaga agaagaggaa ctcttttttg ctcatttagc gcgaaaagta
    10321 gctgcaatca atgcgttgac accaaaattg agccaagaac tggcagaaaa aaaccaagaa
    10381 gatcttttcc ggaaaatgga actgccattg tctatcattt tggcagagat ggaaataagc
    10441 gggatcaaag tagatgcgac tcgcttacaa gaaatgaagg gcgagttctc tgctcgctta
    10501 agagagatcg aacaaaaaat ctacgaagaa gccggcgaag aattcaattt gaattcacca
    10561 aagcaattag gagtgatctt atttgaaaaa atgggattac ccgttatcaa aaaaacaaaa
    10621 actggatatt ccacagccgt agatgtactg gaacagcttc gtgaacaagc accaatcgta
    10681 gaggatatcc tcacctatcg tcaaattgca aaaattcaat ctacctatgt cgagggactg
    10741 ctgaaagtga tccaatcaga tggaaaagtg catacccgtt atgtccagac attgacccaa
    10801 acaggacgat taagctctgt tgatcctaat ttgcagaaca tcccgattcg attagatgaa
    10861 gggagaaaga tacgtcaggc atttgttcct agagaaaaag actggctgat ttattcttct
    10921 gactattcac aaatcgagtt acgcgtactt gcgcatatct caaatgatga acatttgaaa
    10981 gctgccttcc tagaaggtca agatatccat gccagcacag cgatgcgtgt atttggaatc
    11041 gaaaaggcag aggatgtcac accaaatatg cgtaggcagg caaaagcagt gaattttggt
    11101 atcgtgtatg gcatctctga ttacggactt tcacaaaatc taggaatcag ccgaaaagca
    11161 gcacagcaat acatcgatac gtactttgaa aaatacccag gtgtcaaaga atatatggaa
    11221 agaatcgtac gcgaagcaaa agaccaaggc tacgtcgaaa cgctgtatca ccgccgtcga
    11281 tatctgccag acatcaattc aagaaattac aatatccgtt cttttgcgga acgaacagct
    11341 atcaatacac caatccaagg aagtgctgct gatattttga aaatcgcgat gatcgaattg
    11401 gataaacgat taaaagaaac aggtcttcaa gctacgatgc tgttacaagt tcatgatgaa
    11461 cttgtcttcg aggtaccgga aaaagaactt gaatcattag ataaattggt gaaggaagtt
    11521 atggaacaag ctgtttctct tcatgtacca ctgatcactg acagcagttg gggaaaaacg
    11581 tggtacgaag caaaataaac attgtttaag acagagcaat ataatgaagg tgaaaagaaa
    11641 aaatgccaga attaccagaa gtagaaaccg ttcgtaaagg attggaacgg ttagtagtag
    11701 gaaaaacgat tcaaaaagtt caggtcttgt ggcccaagat catcgagcaa ccagagacac
    11761 ccatctttga ggcttctctt gttggcgaaa ccatccaatc cattggacgt agagggaagt
    11821 ttttaatttt tcatctcgat cattgtgaac tgatctcaca cttgcgaatg gaaggaaaat
    11881 atcaatttac gaaagaaaat acaccaatcg ataaacatac gcatgtactt ttcttttttg
    11941 aggatggaag ccaacttcgc tacaacgatg tacgaaaatt tggtcgtatg acaatcgtcg
    12001 aaaaaggagc aagtgcaact taccgaggaa tcatgaaatt gggacctgag cctttgccag
    12061 atagcttctt attagcagat ttcgcaaatg gactgaaaaa gtcccacaaa gcaatcaagc
    12121 cattgctgct agaccaacgt ctagtgactg gtctaggtaa tatttatgta gatgaagcat
    12181 tatgggaagc gaagatccat ccagaacaac cggcgaacac actgcgcccc aaagaggtag
    12241 aacaattacg tctatcgatc attgatgttt tggatcgtgc aatagaagcc ggaggtacca
    12301 cgattcgcag ttatttgaat gcattaggag aatcaggcgg atttcaagta gcactgcatg
    12361 tctatcagca aactggcaaa ccatgtatac gttgtggaac gccgatcgtg aaaacaaaag
    12421 tggcacaaag ggggacgcac tattgtccga attgtcaaag gctaaaaatc gcaaagtagg
    12481 atttgtttta ggtttgacgg gtggaatagc taccggtaaa agtacggcag ccaaagtctt
    12541 tcagtctcat ggatttcctt tgatcgatgg cgatgtgatt gccagagaaa ctgttgagcc
    12601 tggaacgctt gctctgagga aaattgtatc agtgtttggc caagaggtat tgcagcctga
    12661 tgggcaacta gatcgaggga aacttgggat gattgttttt ccatcaaagg aaaaacggca
    12721 aaaacttgat cagctattag atccgtttat ccgaaaagcg ataaaagatc aaatcgcctc
    12781 tctaagttcc aatcatcctt tagtaattgt agatatccca ttgctctacg aaggtcatta
    12841 tgaccactat atggatgcag tagctgtcgt ttatactacg ccagaaactc agcttcagcg
    12901 tttgatgaaa cgtaatcaac tgacgctaga acaagcgcag cagagaatta gtagtcaact
    12961 tccaatcgaa gaaaaaaagc agcgtgcgga tattttgttc gacaacaatg gaacaaaaga
    13021 aatacttgtg ggacaaatcg aaaattggct ttttgctaat cgctttcttt gagtatctgt
    13081 gtcaattttg ctatcctgag tatatagaaa gtgagggata gcgatgggat cattaaaaaa
    13141 tcaaaaggat aaagtcgtag gtaacgcaaa agaaaaaatc ggcgagatgc tcaataaaga
    13201 aaatctaatc gatgaaggtc gtacgcaatc agaaacagca gaagaaaaag aaaagcagta
    13261 ccaagcaaga atcgatgaaa tagaagacca gcaggcagaa gaagaagcga agcggcaaga
    13321 tttgactcaa gaacgaaaaa atccgacacc tgatcgtagt aaaacaccgc tcccagaaag
    13381 tgaccacatt cccgatgagc aaaaaatcga ggaacagcga atgaaacact atacaggaat
    13441 cgaagagaag ttataaaata gtaaaaaatc gagatgtgac ttgaagcaga tgagaaggtg
    13501 ttcatctgct tcaagttttt tgtgctagat gactaaatca gcttttgtgt aagttatttc
    13561 tcagtaatca aggtgatcag tattggaaaa ttgaaattta gtaaggacag gggacacaat
    13621 tacgcatgaa agtagaaaaa attgttctta tatccgtgtt ttttgggtag gacagtcgcg
    13681 tttgtgattt gtttcgtgtt ataatacaac tagttgtaaa gaaagacaca tcctttatat
    13741 aaaatgcgat gcaagaggtg agacaaaatg agatgtccca gatgtcaaca taataattca
    13801 cgagtaatcg acagccgtca agcagatgat ggacgtgcga tccgtcgccg tcgcgaatgt
    13861 gagaattgtt cgttccgatt cactacattt gaacgaattg aagctgctcc gttattagta
    13921 atcaagaaaa acggggaacg cgaagagttc aatcgcgata aaattttaag aggactcatc
    13981 cgttcagcag aaaaaagacc tgtagctatg gaacaaatgg aacaaatcgt caaccatgtc
    14041 gaaaaccgcg tacgcagtct cggagaaaac gaagtatcga cgaatctaat tggtgaatat
    14101 gtgatggaag atttagttga tttagacgaa atcgcttata ttcgttttgc aagtgtttat
    14161 cgccagttca aagatatgtc tgttttctta aaagagttgc aagatatcgt ggataaagca
    14221 aaagaaaata ataaagagaa taataaagaa aacaattaag aaacaaaaag gaggcatttc
    14281 cttggaaaat gcttggaaag agcttcaacc aaaaaatatt taccaaggaa gaaaagcggc
    14341 tccgctgaga gaagacagca agaacgcctt gctgtatctt tatcagccac taatcggcgg
    14401 agaagccttg agtatctatt tcaccctgtt atcagaaatc tctcaagaat ctggcaaagg
    14461 tcctgaaggg atgcatgcag atctgcttag tagtctaggc tgcggtatcc cgcaatttta
    14521 tgatgcaaga aaaaagctag aaggaatcgg cttactggat gtttatttta aagaagatag
    14581 tgatctggga cctcgtttta tttatgagct gttagaacca ctcattccta aagcattttt
    14641 caaagactct gttctggctt ttcttcttct ggaaaaagtt ggtgaacgac gattccagaa
    14701 gcttacccaa ttctttgaac ctaaaaggca tacagcagag ggatatcaaa aaatcacaaa
    14761 aaaattttta gatgtttatc gcttcaatga agcagtgtac gctgctaatc aagaaaacat
    14821 cgaatcgctg aataaatctt ttgaagaatc tgaatcagta gcattacttg aagcagattc
    14881 gacattcgat tggcagtttt tgaatgattg gctggcaaaa aaacatattg caagtcttga
    14941 tgatccaatc atcaaacaag tcgacatgta tcatcagctt tacggtttcg atgaattgac
    15001 acttggcgaa ctgatcagtc aatcttatga ctttagtgaa caaaaagtat ctgaaaaaga
    15061 actgcaaaaa gccattttgg caatgcagag acgtccggtg aagcaaacaa aagaaacagt
    15121 tgcggtttct gaaaacagta atgaaccctc gtcaaacgaa catttgacaa acgcagcgct
    15181 tcagctgata aaagaagcac agagcattcc accgatgaaa tatctggaag caatcaagtc
    15241 agaaaaaggc ggatatgttt ctaaacaaga aaactggtta ctgcaagatc ttgtttctca
    15301 atcaggtctt tccagtagcg tgatcaatgt tttgctgaat tatgtactag tgatcaagaa
    15361 taatgctagc ttaaatgcca gttatgtaaa tacagtggca aatgaatggg cacaaaagaa
    15421 aatcgccaca gcagaagaag cgatccagca cattcggcaa ataagtaatc aagcaaaaca
    15481 atcaaaacaa aagaagcaaa caaattatca aactggcaag cgaaatgttc gacgggaaaa
    15541 gcttcctgat tgggtcaacc agccaaaaga tgaaaaacag atcagcagcg agaaaaaagc
    15601 agagattgac cgccgtttta aagaatacct ggcaaaaaag gagggtgata tctagtggaa
    15661 gacgtaggca aagaattgaa gaggatcatc agcaaacgca attatcaaga aaagtatgct
    15721 gaaatgatcg ctgaagtctt aaatgatgcg gatgttcaag cttttctaag cgcacaccaa
    15781 gaagaattga cacaagcgga tattgaaaaa agttacgcta aattgtatga attcgtgcaa
    15841 gaaaagcgta aatatcaaaa aaacgatcca acgatgatcg ctcccggcta tgagccgaag
    15901 ctgacgttga attttcatta tgtggatgtc acttatgtac caacggaagc attgttagca
    15961 cggcaaagac aagaagaaat cagaagccga gtaaaagcaa tggatatgcc aaaagatatt
    16021 caatcggctt cttttgaaaa ctttgaacgt acagatggac gaggatatgc gagcttagaa
    16081 gccctagatt ttgtagagaa atatactgct gatccaaaag ggtatcataa aggactgtat
    16141 ctagtaggaa gttttggtat tggaaaaaca tatttattag gagcaacagc gagagaatta
    16201 gcgattgctg gattcaccac gacgctagtc cattttccga catttgccgt tgagatgaaa
    16261 caggcaatcg gaaaagatca agtggcagaa aagttagatg cagtgaaacg ttcacctatt
    16321 ttgatgatcg atgacattgg tgcagatgcg atgagtagtt ggatacgaga cgaagtattc
    16381 ggtgttatcc ttcaatatcg gatgcaggaa cagttaccaa ccttcttctc atcgaatttt
    16441 acaatggatg aattggaaca gcatttgtcc gtcactcaac gtggagatga agagccgcta
    16501 aaagcgaaac ggataatgga acgcatacgt tatttgacaa aagaaatcga aatgactggt
    16561 cgcaaccgac gcaatggttg aacagttgac aagtccaact gacatttgac ctagtagtaa
    16621 aagcaggtca gcgaatcatt atatggacag aaatgaccgg agacaatcac cgcaacaagt
    16681 aatgcagaaa cttaaacgga ggatttttta tgagggctat tttttctagt atatctgtag
    16741 ttggtctatt ttttctttta gcaggctgcg gtagcgcaga tagcaacaca ggttctgttg
    16801 attcgattga tgcgggtcaa acagtaaaaa caagcagtac gaaaacggag agacaaagca
    16861 tatttgtcgg aacgattgaa agtatcactc aagtagaagc agaagaagaa acgcttcagt
    16921 tgaaactcgt cgatgtcatg gccaaagaag atccagcgaa tatgggaacg tcattcagca
    16981 atgacggagt gatcttaaat gctactgctg aaaaaataag cgtggacata aatgaattga
    17041 aaatcggtga caaagtccaa tttacattag ttgagatgcc gatcatgacg atgtctatcc
    17101 caccgcaagt tcctggagat tcgattattg atgtttcatt gaacaaataa ccaataacat
    17161 tttttcaaaa ataagaaaat gttgttttat agaacgaaaa ggtatatttt ttaaggagat
    17221 atgggaattt ttttattcct agtctcctta ttttatttgg aaaatgctat aattatctag
    17281 aaggagtgaa gctatgaacc atttagattt atctcagcgc ttagccgcag tgggtgactt
    17341 tgtccctgaa tccgctcgtt tagcagacat tggatcagat cacgcttact tacccgttgc
    17401 attgatatta agaggaaaaa tcgaatatgc agttgctggt gaagtcgttc aaggtcctta
    17461 tgaatcagca cgtaagcaag tcaaaaaaga tggattagaa gaccgtatca ccgtacgcct
    17521 tgcaaatggt ctagatgcta tccagccaca agatggtatt acagctgtca cgattgcggg
    17581 tatgggaggt tcactgatcc gagatatcct agaagctggc aagttgaatc aacgcttaag
    17641 cggacaagaa cgtttgatcc ttcagccaaa catcggagag cgtacattac gatcttggct
    17701 gcaagaaaat cagtataaaa tccaatcaga aaaaatactt gaagaaaata aaaaaatata
    17761 tgaaatcatt gtagccgaaa aagaacaaaa accagttact tacaatgaaa tggagctgat
    17821 gttcgggcca tttctgcttg aagagaaaag tgagatcttc aaatcaaaat ggacaagaga
    17881 gcttcaacaa agaaaagcta tccgtgaaca gctcaaacaa gcagctaatc aagaggcacg
    17941 tcaaaaacaa atcgagcagg aaatccaaat gattgaggag gttttaaagc catgacaatc
    18001 agcggacgca ctttcatcca aaagtttgaa agctattgtc ctcagtggct agctgaagaa
    18061 ggagacccag tcggactgca tatcggaaca ttggataagc caatccaacg agtaatgatg
    18121 acattagacg ttcgtccaga agtggtcgaa gaagcaatca agaaaaaaat cgatttgctg
    18181 attgctaaac atcctccaat ctttcgtcct gtaaaacggc tggttactga tcagccacaa
    18241 gaaaaaatgt atgcagattt gttgaaacat gatattgccg tctatgcagc acacacgaac
    18301 atggatatta tttgggatgg actgaatgac tggttttgtg aacttctcgg tattgaagtg
    18361 gagagttatt tagtaaagac tcatgagatt cattataaaa aactggccgt ttatgtacct
    18421 gtggatcatg cacagaaaat gcgtgaagta ttagctgcag ctggtgctgg tacacaaggt
    18481 gattataccg gtacaagttt tacctcgatt ggacacggtc gatttactcc ggaagctggt
    18541 gcacaaccag cgattgggaa agtagggaag acagaacaag ttcaagaggc aaaagttgag
    18601 gttattttgc cggaaactat cgaaaaacaa gtgattcaag ccatgcggtc agctcatcct
    18661 tatgaggaac ctgcttatga tcttttcgct atagatgaac cagtcgaaat gtttgggtta
    18721 ggacgagtag gtgaattgcc gcaagaaatt tctatagagg catttgtaga acaagtaaaa
    18781 gaagcgttcc aactagatgg actgagaata gtccagccaa aaaatgcaaa atcctctgtg
    18841 aagcgcattg cgatttgcgg tggaagcggt gaaaaatttt atccgcaagc aatcgcccaa
    18901 agagcagatg tctatatcac gggagatatc tattatcata cggctcatga tatgcaaagc
    18961 gctgggttga ttgcaattga tcccggtcat tatattgaat cgttatgcaa gcaacgattt
    19021 atcgagaaat ttgaatcttg gaaacaagaa gaaaattggg atattaactt tttcgtttct
    19081 gaaacaaata caaacccatt tcaatttaat taaaaggaga gaatactatg tacgaaaatc
    19141 ttttgccacg ttttttacga tatgtcaaaa cagaaacacg ctccgatgca acaagcaaaa
    19201 cgactccatc gactcagaca caagttgctt ttgcgcaaga attgaaaaaa gaattagaag
    19261 agttaggctt agctgatgtt ttctataatg aagataacgg attcgttatg ggaacattac
    19321 caagcaatgt tgatcatgaa gtgcgttcca ttggatttat ttcccacatg gatacagcag
    19381 attttaatgc agtaggagtc aatccacaaa tcattgaaaa ctatgatggc gagtctacga
    19441 tcgtactaga tgcggaagaa cgctatacat tgaatacaaa agattttcca aatttgaaaa
    19501 attatgaagg ccacacattg atcacaacag atggcagtac attactggga gcagatgaca
    19561 aatcagggat tgcggaaatc atgactgcaa tggagatctt gatcaataat ccttctatcc
    19621 ctcacggtac catcaaagtg gcttttggtc ctgatgaaga aatcggtgtc ggagcggata
    19681 aattcgatgt tgagcaattc gatgtagatt ttgcttatac agtcgatggt ggtcctttag
    19741 gtgagttgca gtatgaaacg tttagtgctg ctcaagcaaa tattacgatc caagggaaaa
    19801 atgtacatcc agggacggca aaagatacaa tgatcaatgc tctacagtta gcaatcgatt
    19861 ttcataatca gcttccagcc gatgaagttc cagaaaaaac ggaaggatac gaaggtttct
    19921 tccatttgat ggcattggat gggaatcctg aagaagcttc tatgagttat atcattcgcg
    19981 atcatgatcg tgaaaaattc gaagcacgca aagcacaaat cacacatatc caagaagagt
    20041 tgaatcagcg atttgatcaa gaacgaatca aagtagatat gtatgatcaa tattacaaca
    20101 tgaaagaaat cattgaaaaa gatatgagca tcgtggaatt agcgaaacaa gcaatgatcg
    20161 agcttggtat ctctccagtg atcgaaccag ttcgtggagg aacagatggt tcaaaaatct
    20221 cttacatggg tattccgacg ccaaatctat ttgccggagg agaaaatatg cacggacgat
    20281 tcgaatttgt ttctttacaa gtaatggaaa aagcaacaga cgtgattgta aaaattgcag
    20341 aattgaacac aaagagttaa aatcgaaaaa gaacagctta tgacaaggcg ttcgtcccaa
    20401 tgatggaatg tcatctgtct aaggctaaag ggcaggacaa tggaattggt tgtcccgccc
    20461 tcttttttga agagtaaata ggaggtaaag aatggaaaag ctgctagttt tttataacaa
    20521 aagttcaggg aaagatgagg gtgagaaatt agcacgctgg tttcaagatt atgtaaaaga
    20581 aaagcagcca gaattatatg tagaactagc tgaaactggc ccatcgattg atgatgcgga
    20641 attagtcaag aaagcagaag ccatagaagc tgatacttta gtaattatag gtggagacgg
    20701 cacgattcat catatcgtcc aagcgttcca aaaaacatta tctcaatacc atgtcggatt
    20761 acttccaggt ggaactgtca acaaccttgc acgtgttctt gagattcccc tcaaaaaaga
    20821 agaagctgct gacgtgatcc tggaaggcaa tgtacggaaa atagactatg gtcaagtcaa
    20881 tgatgaagtc attatctcta cactgacaat cggaatctta gcagatacag cagtttggat
    20941 cagtcaagaa gaaaaacagc gttacggctc gtggatcttt atcaagaagt tttttaagtt
    21001 attgctgaag aagaaaaaat atcatttgga tttagaaaca gacgaggagc attggaaagg
    21061 aaaatcccaa ctgttgacag tcacgatgtc taactccgtg ggcggtttta caaattttga
    21121 cgactcagcg accctagatg atggaaaatt tcatgtaacg atcgtacctt ccttagatat
    21181 tttccgattt attttttatt tgcctagaat catgagaggc aaattctata cgatccccgg
    21241 cattacttat ttcacagcca aacgcatgaa aatccaagcg caggaaaaat ctgtgggcac
    21301 tcgaacggat ggagacacga cagatgatct tccagttgaa ttatctgttg tatcaaaagg
    21361 attaacagtt tattcaccaa agaaagaaga aaaagcttag aaatagtagt taacagaaag
    21421 ttggaagaag agtaatggat gtattgttac acaatccttt gtggttttac cttttcattg
    21481 tgaattttta cttattttgt ctgatggggt atgataaaca tcaggctaga actagcggct
    21541 ggcgtgttcc tgagaataat ttgatatttg tcagcctgtt aggcggtggt ttgggaggat
    21601 tgatctctcg aaaagttttc catcataaaa ctcggaaaaa gaagtttact ataggattcg
    21661 ctgcaggtgt acttgttgat ttgattctca tttatttttt ccattaagga gcaggagatg
    21721 tcgtctaaag gaacgaaaac taaaatcatt ctgaatctgc ttttgctgat tgtgattttt
    21781 gccattattt attatttaat caatcaatca tttgcagata tctttgccga attgctgtct
    21841 accagtgttg ttgttttggc agcggctgtt atattaggga cagtttacca aatcgctgaa
    21901 gggcgctcaa tcaaagaaat tgctgaacct ttcgcgccga attttacaac gaaagatggg
    21961 ttcttgacct ctagctatat tgcgttttac cgaatcgttt cgtttggtac aggaacgctg
    22021 atctcggaga tttattttta taacaaaaaa ggaatgaagc tatcccaggg agcaggggtg
    22081 acagcactcc atatgattat gtacaaactg gcagtgatca cttatgcatt agctggcttg
    22141 gtcatccaat tctctctttt ttattctaat ggtccatcaa tgatctggtt tatcatcgct
    22201 ggtatccttt tgacgatcgt gattgttgcc tttttattgg cgatgtccgt cagcttgaat
    22261 gttcaaatat tttttgtcag cttatcgaat aagttattta aaagtgagcg tttgcggaaa
    22321 tgggtggata catgcaatca gcagatctac tctttaaggg aaacggtcca aaccattatc
    22381 caagacagga ctgcgctatt gcggatttat ctatggaata tgttaaaact tgtattttgg
    22441 tatgtacttc cttatttgat tctgattgaa aaccatccta aaatagattt tctactgacc
    22501 atttcgttta tcagctttgc agtagtgctt tcaggggtta ttcctacgcc tgctggtatt
    22561 ggttcctttg aatttgtcta tttgttatta ttcagaccag ttgttggaac agttgacgca
    22621 gtttcttcgc ttttactgta tcgtttcagt tcctttatct tgccatttct ctatggattt
    22681 gtgtatgtga ttgctgaacg gcgaagagtg atcaaagaag aaataataga agtaaaaaaa
    22741 gagaataaag aaaatcaagg gtaggtcttt tataagatct acccttttta tcaaatcaaa
    22801 gaaaaacgtt atggataaga taattttacg tttgtttggc tcttttttta tatacataca
    22861 atcaattttg tttttttaag atttatataa gtcgagaaaa aaagtcaaaa aactatgtgt
    22921 atattttgac caaaattgac caaagcgata taatagtttt tgtaaagatc aaagaaataa
    22981 gtgatcttac ctttaattag gagggtatac ctgtatgaat attgagaaaa tgactacgac
    23041 gcttcaagaa gcgattgctg aagctcaaca gattgcggtc actcgaaaac atcaagatat
    23101 cgacattgcc catgtatgga aaatcttttt acaatctaat cattttggtc gaaatttcta
    23161 tacggatgcc ggattggatg tcgaatcttt tgaacatgaa attgatcgtc tactggatga
    23221 gtatccagtt gtttctggtg gaaacgtcca atatggacaa aacctcagcc agaatttctt
    23281 tcgattattg aatgaagcag accaaatacg tgaaagcttc ggcgacgagt ttctttcaac
    23341 ggaagtcgtt attttagcgt tgatgaaatt aaaaaattat ccgttaactg tttatttgaa
    23401 taaaaacgga ttatctgaaa aagaattgcg taaaaacatc gaagagatga gaggaggaga
    23461 aagagtgacc tcacaaaacc aagaagaaca atacaaagca ttagaaaaat acggcgttga
    23521 tttagtacag caagtcaaaa gcggaaaaat ggatccaatc atcggtcgtg atgaagaaat
    23581 ccgtgatgtc atccgtattc tttcacgaaa aacaaaaaat aatcctgttc tgataggaga
    23641 acccggtgta gggaaaacgg caatcgttga agggcttgct caacgtatcg tacgcaaaga
    23701 tgtgcctgaa aatttaaaag ataaaacgat tttttcatta gatatgggtg cattgatcgc
    23761 tggagcaaaa ttccgtggtg aatttgaaga aagactaaaa gcagtcttga aagaagtcaa
    23821 aaaaagtgat ggacgtatcc tcttgttcat cgatgaaatc cacaatatcg taggggcagg
    23881 aaaaacagaa ggaagtatgg atgccggaaa tctgttgaaa ccaatgcttg ctcgcggtga
    23941 attacatttg atcggtgcca caactttaga tgagtaccgt caatatatgg aaaaagacaa
    24001 agcgttggaa cgtcgctttc aaaaggtatt agtcaaagaa ccaacagtgg aagatacgat
    24061 cagtattctg cgtggattaa aagaacgttt tgagatccac catggtgtta acatccatga
    24121 caacgcatta gtagcagcag ctactttatc tgaccgttat attactgatc ggttcttacc
    24181 ggacaaagca atcgatttga tcgatgaagc cagtgcaacg attcgggtag aaatgaactc
    24241 tatgccaacg gaattagatc aagttactag acgtttgatg cagttggaaa tcgaagaagc
    24301 tgcactgaaa aaagaatcag atgatgcaag taaaaaacgc ttgaaaaatt tgcaagaaga
    24361 attagctgaa cttcgtgaag aagcaaatgc gatgaagatg cagtgggaaa cagaaaaaga
    24421 agaagtcaat tctgtttcag caaaacgtgc tgaaatcgat aaagcaaaac acgaattaga
    24481 agatgcagaa aataactatg atttagaacg cgctgctgta ttgcgtcatg gtacgatacc
    24541 tcaattagaa aaagaattga aggaattaga agctaaagca aaagacagcg agattaagat
    24601 ggttcaagag tccgttaccg aaaatgagat tgctcaagtc gttggcagat taacgggtat
    24661 ccccgtaacg aaacttgtag aaggtgaacg agaaaaacta attaaattga atgaaacact
    24721 tcataaacga gtgattggtc aagatgaagc agttgatgcg gtcagtgacg cggtaattcg
    24781 ttcacgggct ggattgcaag acccaaatcg tcctctaggt tctttccttt tcctaggacc
    24841 aactggtgtc ggaaaaaccg aattagcgaa agcgttagct gagaacttgt ttgactcaga
    24901 agatcatatg gtacggatcg atatgagtga atatatggaa aaacatgctg tgtctcgtct
    24961 agtcggagct cctccaggct atgtaggtta cgaagaaggt ggtcaattaa cggaagctgt
    25021 cagacgaaat ccttatacaa tcgtcttgtt ggatgaaatc gaaaaagcgc atcccgatgt
    25081 cttcaatatc ttgttacaag tcctagatga cggacgcttg actgattcaa aagggcgagt
    25141 agtagatttc aaaaatacgg ttctgatcat gaccagcaat atcggttcac agttactgct
    25201 ggaaggcgtg actgctgatg gaacgattcc agaagcagtg gctgaacaag tcaacacgct
    25261 attacgtgga aacttcaaac cagaattctt gaaccgaatc gacgacacga ttttatttac
    25321 accattgagc ctagacaacg tgaaaggaat cgttgacaaa atggtggcgc aattggcaca
    25381 acgtctggaa catcaagaaa tcctgctgac aatcagtgat gaagccaaaa cgtggattgc
    25441 tgaaaatgct tatgaaccag catatggtgc aagaccattg aaacgattta tcactaagga
    25501 agtagaaaca ccactagcta aagaaatcgt tgcaggacat gttatgccta aatcaaaagt
    25561 aacgattact ttacttgatg gacaacttca tttcaaaaca gaagagctag aagaaatcgt
    25621 ctaatcttcc attgagacta taaaagaaaa aaggggtctg acacaactag tgtcagacct
    25681 ccttttttca aataaacagt gttccagaag tagtttcttc gaaaaataag ccgaaatttc
    25741 ttgaagctgc ccgcttctgt cacaacctct tttattaggt atttttcact ttttctaatt
    25801 tataactttc aaagtaacgc tcatcttttt tactttgaga ccatgcaaac aatcccatta
    25861 aggcagcacc tatcgtattt gtcagcaagt cgcccatcgt atccatcaat gctgctcgtc
    25921 cgacaaacag tgtgccatca gaagcagcaa aacgttgaag attcatgcct aagaactggt
    25981 cgcataagaa ttcccagaac tcccaaaaca cgccgcaaag tccagcaaac gcaaagccga
    26041 ataagaggaa caaccaagga ctggtttttg atatctcagc atctttcatc aagtaaccaa
    26101 tcaatccata gccaagagct gtcaggacca ttggactcac cgcatgtaag attttgtccc
    26161 aaaaagagat gatactgatc aaatgcatcc ctgtgcctaa aaagacagag atgaacaaga
    26221 aaaaccagta aaacaaaacg attgctgctg gtattttaag tttgaaaact ttcaaaatga
    26281 cttgtggcat gaaaatcaat gcaatcccta ataaagattc cacgataaat gtgattcctt
    26341 gagaagtcga atatttttgt gtgataaatt cataaaaatt atagcagaca acgaatacac
    26401 caaaagcaag taaagctatt tgcacctttt tgatttcttt aatcactgaa agtccctcca
    26461 tctaaaaatc tttttacttc tgaaagcaac tcaatttctg aagaaaccac ttttccgttc
    26521 aatttgatca aacctattgt atataagttc aggtagtgaa attgattttc agctgtttct
    26581 tgtagtgcag cgattttttt cgggtgggtt ccgccttgct gtcgagtatc agtatataag
    26641 ccgatcactg ggatgccttt cgcataagcc acaccgattt ccgatgctac cccggcatcg
    26701 atcgtcagcc catctagtaa agcaatcatt aaatcactag ccaacacttt ttctgtgtca
    26761 gcaagagcaa tcattcgact gtctgcataa gcagttttgt cattgattgc ttcattttct
    26821 tgaggaagat agatcgaaag ctcagaagag agctctctga tttctttaac taactgttgg
    26881 ttatataaca aatcactttt agcaaacatt ggtgcggcaa aataaatttt catctaaaca
    26941 tcatcctttt tatcaaaaat aatcgctaaa gtaagcttcc atgtttggaa tgatacgctc
    27001 tagtgacttc agtgtttctt catctgtttc taaccgttcg attcttgcca aataagaagc
    27061 acggcgataa ttcatcatca agcaagtcaa tgtttggata tccagtcgaa cggattttcc
    27121 tataggttca tttaagacac ggacttgtcc ctgttcatcc catgttaaag cgaagacacc
    27181 attattccaa ggagcgacag gatcttcaat gatgaaatga aaagcatcag ctgttccaac
    27241 aaacggaaac cgttgaagga attcttttac atcaacgatc cgagccataa aatatggttc
    27301 gatctgctct ttgatctgac tatcttcaat caaaaatgct aaaggctcat ttttataaat
    27361 atctccatgt acccagtaga tcatggaaaa atgtgcggaa ataaagttcc ataagccatt
    27421 tcgagcttct tgatcaagat agaacatttc ttttacgtga aatatttctt cagctaccca
    27481 gtagaacaga acacctcttg gttcatggtt gctatcgtag tagacagcgg ctgtgcgctc
    27541 atcttcattt tcgaaacgcc aatactcttc ccaatgaaaa tcactgcgga ataaagcacc
    27601 atggttttgt tgggcaaatt tgtcataaac ggaaaatacg tctggatgat caactggtaa
    27661 tcgctcaacc ataccactta ctgggatttg tttaggtagc tgtgtatctc tgattttaaa
    27721 ggaaagttta tcagacatga tttcccatcc tttacgccga tagtagggga tactgtaagg
    27781 atacaaataa gagatccatt gcttattctt gcgcatatta tccaatgcaa cgataattag
    27841 atcttgcatc aatccatggt tagcatattc tggataagtt ccaactccag tcacaccacc
    27901 cattttataa cgtgcaccgt gaatattgac ttcacaagga tagatagcga tttgagagat
    27961 cagtttattt tcgtggaacc atccgaacac tttcgatact tccaaaatcg gttttttgga
    28021 acggataaaa gctcgtttat tctcaaaccc gctttcctcg atgtctgctt cagtcacttg
    28081 gaacacatag gaaagcaatt cattgaattg atcgatatgt tcttgagtga ccggttttaa
    28141 ttcaagattt tctctaaaat ctttttgatc cattttttca tctccattta ttagcttgat
    28201 aacagtatag caaaattcta caaaaaaaac ttctatagct gactaattaa aaactcttta
    28261 aattactgct tttcgttggt ataatggaaa gtgctgatta tatgaaaaga ggagacgcta
    28321 tggatataaa gaaaatgaaa gaacgccaag agcagattcg taatttttcg atcatcgctc
    28381 atatcgacca tgggaaatct acattggccg atcgtatttt agaaatgacg cacactgtga
    28441 cgtctcgcga aatgcaagat cagctgttag actcgatgga tttggaacgt gaacgtggaa
    28501 tcacgattaa attaaatgct gtagaactca attacacagc gaaaaatgga gaaacatata
    28561 tctttcactt gattgacacc ccagggcacg tcgacttcac ctatgaagtc tctcgaagcc
    28621 ttgctgcctg tgagggtgcc gttttagtgg tcgatgcagc acaaggaatc gaagcacaaa
    28681 cattggccaa tgtttattta gcgttagaca atgatttgga gatcctgcca gtcatcaata
    28741 aaatcgacct acccgcagca gatcccgaac gtgtccgtca agaaatcgaa gacgtaatcg
    28801 gaatcgatgc tagtgaagct gtattagcca gtgccaaatc agggattggg attgaagata
    28861 ttctcgagca gatcgtggaa tatgttcctg caccaagtgg agacttagat gcaccgctaa
    28921 aagctttgat ctttgactct atttatgata gttaccgcgg tgttgttcta aatgtccgga
    28981 tcacggacgg tatggtcaaa cccggagaca aaatcaaact gatgagtaat ggcaaaacat
    29041 ttgatgttac agaggtcggg gtcttctcgc caaaagcagt tgctagagac tttttgatgg
    29101 tcggtgatgt cggttatatc acagcaagta tcaaaactgt ccaagataca cgagtagggg
    29161 atacagtaac gttagcagac aatcctgccg ctgaagcttt accaggttac cgaaaaatga
    29221 atccgatggt ttattgtggt ctttatccaa ttgatacttc tcgttacaac gatttgcggg
    29281 aagctttaga aaaactgcaa ttgaatgatg ctgctttaca attcgaacca gaaacctctc
    29341 aagcattagg ttttgggttc cgctgtggat tcctcggact tcttcatatg gatgtcgtcc
    29401 aagaacgctt ggaacgggaa ttcaatctag aattgatcac gactgcacca tccgttattt
    29461 accatgttaa taaaacggat ggtacgactg ctacagtaga taacccggca gattttcctg
    29521 aacctgtaac gattcaggat gtagaagaac cattcgtcaa agcacagatc atggtcccaa
    29581 atgattttgt cggtgcagta atggaacttt ctcaacgaaa acggggagaa ttcattacga
    29641 tggattattt agatgattac cgagtaaatg tcgtctataa tattccgctt tctgaaattg
    29701 tctttgactt tttcgacaaa ttgaaatcaa gcacgaaagg ctatgcctca ttggattatg
    29761 aaatgtctgg ttatcaaaaa agcaagttag tgaagatgga catcttgctg aatggagaaa
    29821 aagtcgatgc attaagcttt atcgttcacc gagactttgc ctatgaacgt ggaaaagcaa
    29881 tcgttgaaaa actgaaaaaa ctaatcccac gccaacaatt tgaggtgcca atccaagcag
    29941 ctattggaca aaaaatcgtt gcccgttcag acatcaaagc cttgcgtaaa aacgtcttgg
    30001 ctaaatgtta tggtggagat gtttctcgta aacgtaaact cttagagaaa caaaaagaag
    30061 ggaagaagcg gatgaaacaa atcggctccg tcgaagttcc gcaagaagcc ttcatggccg
    30121 ttctgaaaat ggacgaggac gaaccgaaga aatagcaagg gtttgtttga aaacagtgta
    30181 aaaaataaac catgtttaca tggatttgcc accggtttgc caccggtg
//
LOCUS       DAYYCY010000015        30130 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000015 DAYYCY010000000
VERSION     DAYYCY010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30130
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.015"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(40..618)
                     /locus_tag="ACHAZB_000551"
     CDS             complement(40..618)
                     /locus_tag="ACHAZB_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFM8613251.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(695..1225)
                     /locus_tag="ACHAZB_000552"
     CDS             complement(695..1225)
                     /locus_tag="ACHAZB_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HFM8613252.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(1229..2089)
                     /gene="rlmB"
                     /locus_tag="ACHAZB_000553"
     CDS             complement(1229..2089)
                     /gene="rlmB"
                     /locus_tag="ACHAZB_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFM8613253.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(2082..2477)
                     /locus_tag="ACHAZB_000554"
     CDS             complement(2082..2477)
                     /locus_tag="ACHAZB_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HFM8613254.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(2474..3886)
                     /gene="cysS"
                     /locus_tag="ACHAZB_000555"
     CDS             complement(2474..3886)
                     /gene="cysS"
                     /locus_tag="ACHAZB_000555"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFM8613255.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(3883..4419)
                     /gene="epsC"
                     /locus_tag="ACHAZB_000556"
     CDS             complement(3883..4419)
                     /gene="epsC"
                     /locus_tag="ACHAZB_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HFM8613256.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(4505..4744)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(4741..6201)
                     /gene="gltX"
                     /locus_tag="ACHAZB_000557"
     CDS             complement(4741..6201)
                     /gene="gltX"
                     /locus_tag="ACHAZB_000557"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFM8613257.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(6371..7528)
                     /locus_tag="ACHAZB_000558"
     CDS             complement(6371..7528)
                     /locus_tag="ACHAZB_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HFM8613258.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(7562..8932)
                     /gene="radA"
                     /locus_tag="ACHAZB_000559"
     CDS             complement(7562..8932)
                     /gene="radA"
                     /locus_tag="ACHAZB_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFM8613259.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(8995..9522)
                     /locus_tag="ACHAZB_000560"
     CDS             complement(8995..9522)
                     /locus_tag="ACHAZB_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFM8613260.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            9752..10558
                     /gene="proB"
                     /locus_tag="ACHAZB_000561"
     CDS             9752..10558
                     /gene="proB"
                     /locus_tag="ACHAZB_000561"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFM8613261.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            10555..11802
                     /locus_tag="ACHAZB_000562"
     CDS             10555..11802
                     /locus_tag="ACHAZB_000562"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFM8613262.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            11878..12222
                     /locus_tag="ACHAZB_000563"
     CDS             11878..12222
                     /locus_tag="ACHAZB_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8613263.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(12339..12665)
                     /locus_tag="ACHAZB_000564"
     CDS             complement(12339..12665)
                     /locus_tag="ACHAZB_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8613264.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(13280..14482)
                     /locus_tag="ACHAZB_000565"
     CDS             complement(13280..14482)
                     /locus_tag="ACHAZB_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8613265.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            14599..15114
                     /locus_tag="ACHAZB_000566"
     CDS             14599..15114
                     /locus_tag="ACHAZB_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM8613266.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(15313..15759)
                     /locus_tag="ACHAZB_000567"
     CDS             complement(15313..15759)
                     /locus_tag="ACHAZB_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFM8613267.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(15806..17284)
                     /gene="msr(C)"
                     /locus_tag="ACHAZB_000568"
     CDS             complement(15806..17284)
                     /gene="msr(C)"
                     /locus_tag="ACHAZB_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFM8613268.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(17638..18267)
                     /locus_tag="ACHAZB_000569"
     CDS             complement(17638..18267)
                     /locus_tag="ACHAZB_000569"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFM8613269.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(18251..18715)
                     /locus_tag="ACHAZB_000570"
     CDS             complement(18251..18715)
                     /locus_tag="ACHAZB_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM8613270.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(19202..20470)
                     /locus_tag="ACHAZB_000571"
     CDS             complement(19202..20470)
                     /locus_tag="ACHAZB_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFM8613271.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(20701..21438)
                     /locus_tag="ACHAZB_000572"
     CDS             complement(20701..21438)
                     /locus_tag="ACHAZB_000572"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM8613272.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(21505..22074)
                     /locus_tag="ACHAZB_000573"
     CDS             complement(21505..22074)
                     /locus_tag="ACHAZB_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFM8613273.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            22202..23074
                     /locus_tag="ACHAZB_000574"
     CDS             22202..23074
                     /locus_tag="ACHAZB_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM8613274.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            23416..24288
                     /locus_tag="ACHAZB_000575"
     CDS             23416..24288
                     /locus_tag="ACHAZB_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM8613275.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            24285..26243
                     /gene="nagE"
                     /locus_tag="ACHAZB_000576"
     CDS             24285..26243
                     /gene="nagE"
                     /locus_tag="ACHAZB_000576"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HFM8613276.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(26575..27426)
                     /locus_tag="ACHAZB_000577"
     CDS             complement(26575..27426)
                     /locus_tag="ACHAZB_000577"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM8613277.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            27549..28016
                     /locus_tag="ACHAZB_000578"
     CDS             27549..28016
                     /locus_tag="ACHAZB_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFM8613278.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(28279..28761)
                     /locus_tag="ACHAZB_000579"
     CDS             complement(28279..28761)
                     /locus_tag="ACHAZB_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFM8613279.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(28861..29724)
                     /locus_tag="ACHAZB_000580"
     CDS             complement(28861..29724)
                     /locus_tag="ACHAZB_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFM8613280.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
BASE COUNT         8604 a         6373 c         5455 g         9698 t
ORIGIN      
        1 aataaaaata aataagaagc tcattaagat ttttctaaat tattcctcat tataaaattt
       61 caagtgtttt tttattttga gttttactct cccgtaagcg ctcattactt gcttgtgatc
      121 cattgctaaa cattcagcca tgtctacaaa atcaacgcct tgcatataat aatgaaaaat
      181 tttagcttct atttttgaaa actggatttc gcaattttcg attgcttctt ttaataaaag
      241 atgctgaatt gctaaaggtt cttgcaatgt gatctctgct aaaaagtctg gtccttctgt
      301 ctctattttt tggtcgattg agacttcatc tttttgatca cgacgcttca gcgcgttctg
      361 cttcctcaac agagaataga tttgattgct tataacgctt ttataataac ttccaaagct
      421 aacacctttg cttttttcgt aagttctcaa tgcctgatat aaagcgatcc ttgcttcttg
      481 ttcccaatcc tcagaaacat aatttcttaa ataataggtg tttttcaaac gtagtaaaag
      541 tggaaaatat ttttgataca gggcagcaaa tgcttcttcg tcaccattac gtgcagcgcg
      601 caataactcc tcagacataa aataattcct ccataaattc gtttatggag gaatcttatc
      661 atcaatgatt catcgaactg attagaacga tatgttattt attttttctc attaaatcgt
      721 ccaatttttg ggacaatttt tttaactgct cttcattcca tggagaatta cgtcggaaat
      781 caataaaatg catatcttta ttttcttggg ctattgtttt ttccgttttt tttatcgtct
      841 tatataattc ataagcagaa gttcgtaatg ctccttggga aaagatcatc cactgttctg
      901 ctaaatcgct tgtcgccact gtcacttgtg tcaaacggtc attcagctga ccagcgattc
      961 tctcgatata actgtcagcc gtttcgtctt ctttcgtaaa aacaacatcc aactggtatt
     1021 tcttataatt ttgttggatg cccggaacta gttgggcatc gaagacaacg atgacttcca
     1081 ttccttcata tttcgcataa tttgataaac gatgtaaaag agcttctctt gcatccgcca
     1141 ttttgtcctg ctttttaagc tggacaagtt ccggccacga accaatcatg ttgtagccgt
     1201 ctacaatcaa cagctgtttt ttcatggtct actccccctg acgttgggaa aaagcctgat
     1261 acataagcaa accagcagca acactggcat ttaagctctg gacgtgcccg gtcatcggga
     1321 tcgtcagcaa ttcatccatt tcttttttca atcctggact cattcctttg ccttcattac
     1381 caatgactaa agcaattgat cctttggcat tccatttttg gaagtttgtg ccggtcatat
     1441 ctgtgccaaa tacccaaaat cctgtttctt tgagctgttg cacagcttgt accaaattcg
     1501 tcacgcgtgc aacagggaca tgttcgactg ccccagtgga agtctttgtt acgataggtg
     1561 tgattccaac tgctcggtgt tttggtatga tcacaccatc gacgccactt gcgtctgctg
     1621 tccgtaaaat cgacccaaaa ttgtgtggat cttcgatgct atccaaaatc aaaaagaagg
     1681 ggtcttcttt tttctctttt gttcgttcaa tcagttggtc aagggataaa tattcataag
     1741 gtgtaatcgc aagaacgact ccttgatgca cgccgtgatc gctcaaagtt tctaattttt
     1801 gtttaggaac ccatttgact ggcacagctt gttcacgtgc gatttctttc agttcttcta
     1861 ctttttctcc tcgtgaatct tcttgaagaa agagtttatt ccctcgccct tgttgtagcg
     1921 cttctactgc ggcatggtgc ccaaagacga aattatcttc taatgcttct tcattttgat
     1981 cagaaactga ctgatttcgt ccagtttgct ttttatttga atatctattt tgttttttgt
     2041 cttcaaatcc acgtttcgat tgatgtttgt ttgttctttc tttacccatt tttttcacca
     2101 actttctgaa tacaccagcg gatcaattct tccatccgct cggtttgttt tgtcaaatgc
     2161 agatagccca tgagcgcttc gaaccctgtt gcgacccgat acgtcgtaac atcagcattt
     2221 tttgcgctcg tatggctttt agcatttctt cctctgcgat aataaagttc ttcttcttct
     2281 gtcaaaagat gttcttccag catttgctga atcaaaaagg cttgtgcatt cgcagaaaca
     2341 taattcgttg ccgcacgatg cagcttattg ggttttgttt ctccttgatc aatcaggtgg
     2401 tcacgaacat agatttcgta aatcgcatct ccaacataag ccaaggctaa accgttcaat
     2461 tgtgtgtaat ctctcatgct tctcttctcc atcgggttcc ttgcggtgta tcttctaata
     2521 agatcccttt ttctttcaac aaatcacgaa tttcgtcgct tcggacaaaa tcacgattgc
     2581 ttcttgcttg atttcgttct tcgatcaatt gttcaatctc ttcatctaat aactcttctg
     2641 ctgtaaataa aataccgaag atcgaaagcc attgcgagaa taaagcttct ccttgtgcca
     2701 agacagcggc agaaactttc ggctggtcta gataagtgtt cagccactta gcaagctcat
     2761 agaccactgt gatcccattt gctgcattaa aatcatcgtc catttcttcg atgaatcgct
     2821 gttctaaatt ttctaacaaa gcgattttat ccgcatcatc tgccaaattc tctgaggcag
     2881 tttcttggcg gaatttcaaa ttgtcaaacg tattcttcag tcgttgtaaa ttggtggctg
     2941 cttctttcat cgtcgtttca ctgtagcgga tcggacgacg gtattgtgtg gtcgccatga
     3001 agaaacgcaa gacttgtgga tcaacgtttt tgatcatttc atgtacagtg atgaaattcc
     3061 ctagagattt actcattttt tcatcgtctt cgccaatcgt tacatagcca ttgtgcatcc
     3121 agtaattagc gaattttttc cctgttttcg cttcactttg tgcgatttca ttttcatgat
     3181 gaggaaattc taaatcttgc ccgccaccat ggatatcgat cgtttctcct aaatgttttg
     3241 ttgccataac ggaacattca atatgccaac cgggacgtcc tttgccccat ggtgaatccc
     3301 aagaaatctc gccaggtttt gcttgtttcc ataaagcgaa atctaatggg tcttcttttt
     3361 gctgctgttc ttcccctgta cgctgacttg ctccgacttc cagttcatcg atggattggt
     3421 cacttaattt cccataattt tcaaatttac gggtacggta atacacatca cctgaagatt
     3481 cataagcata gcctttttca accaacactt gaataaatgc taaaatatct ggcatatgat
     3541 ccatgactct tggatgcagt gtagcaggtt cgacattcaa cgctttcgta tcttcttcaa
     3601 atgctcggat gaaacgatca gcaacttctg gtgctgtaat acctaattcg tttgctgtac
     3661 ggatgatttt atcgtctaca tccgtgaaat tcgatacgaa atcgacttta tagcctcgat
     3721 attcaaaata gcgtcgaatt gtatcgaaag caatggtgct acgagcattt ccgatatgga
     3781 tataattata cacagtcggt ccgcagacat acataccgac atgtcccttt tgtataggga
     3841 tgaattcttc tttttttctg gtcaatgtat tgtagatttt tatcattctg atctcctcac
     3901 tttttctcct ttgattcgaa cgacttttgc tggtactcct accgctgtag cgtcgtctgg
     3961 aacatctttc aacacaacgg cacctgcacc gattttggcg ttttttccga tcgttaccgg
     4021 tcctaatatc tgcgcattcg cagagagcat ggctccttgt ttaacagttg ggtgtctttt
     4081 acccgtttct tttcctgtgc cgcctaatgt tacgccatgg aataaaatca catcgtcctc
     4141 gatttctgcg gtttcgccaa tgacgattcc cattccatgg tcgatgaaca cgccagttcc
     4201 aatcgttgct cctggatgga tctcaacacc agttaggaaa cgccagaatt gagcattgat
     4261 cttcgccaat aaaaagagcc ggtgctgata taaaaaatga gagaaacgat gccaaaacaa
     4321 ggcatgaaaa ccagggtatg tcaaaacgat ctcagctgtc gaacgtgcag ccggatcgtt
     4381 tctctttgct gcatcaatcg ctcgggtaaa ccaactcatg actttccact cctttcgtaa
     4441 gaattttatg taggaaatca tcttcttata aagaaaagaa gcgcctttta aataagtttc
     4501 cttatcaaaa gacgctttcc gcgtggttcc acttttcttc ataatcaatg attatctctg
     4561 acagctaacg cctgttatac gttcttggct actttgtttc ctataaaaca gtttttcctg
     4621 ttttctgtgt ttcgccaaga ccactcccag aggcatttcg gcaaccattc attgatacct
     4681 tgcaccaccc ggtatctctc ttgtaatgat cagctgctta cttttttctg ttcatcgtgt
     4741 ttagttcagt acttgattta aatgtgctaa tgatttttca cgaccaagca attcgatcgt
     4801 ttcaggcaat tctggaccat gcatttgtcc tgatacagct acacggattg gcatgaaaag
     4861 attttttcct tttacgccag tttctttttg aacagctttg atggcagctt tgacagttgg
     4921 agcatctact acttccattt gttctagctg tgctttgaat gcgtggagaa cagtaggaac
     4981 tgtttcacca gcaagtactt cttttgctgc ttcattcaat acaggatgtt cattgaaaaa
     5041 tagcgtagac agatcaacga tttctgcagc atagctcatt tgaggttgat acaggctaac
     5101 gatcttcttc acccattcga ttgtcttttc atctgggttt tctgctacac gtccatcagc
     5161 gattagataa ggcagacaca tgtcagtaaa tgcatctaga tccattgctt tgatgtaatg
     5221 gttgtttacc cattgcagtt ttttcgcatc gaaagctgcg ggtgatttac ttaggcgttt
     5281 tggatcgaac attttgatca attcttggtg ttcgaagatt tcgtcttcgc ccacaggtga
     5341 ccagcccaat aatgcaataa agttgaacat tgcatctggt agatagccta attcgcgata
     5401 ttgttcgata aattgcaaga ttgattcgtc acgtttgctt agttttttac ctgtttcact
     5461 gttgatgatc aatgtcatat gtccgaaggt tggtggtgtc cagccaaatg cttcatagat
     5521 catcagttgt tttggtgtat tggcaatatg gtcatctcca cgtaaaacgt gagtgatttt
     5581 catcaaatga tcatcgaccg caacagcaaa attataagtt ggcataccat cgcgtttttg
     5641 gatgacaaag tcgccaccga tattatcgga ttcgaacgaa atagcgcctt tcaccatatc
     5701 tgtaaatgcg tattctgtat tgcgaggcac acggaaacgt acaacagatt ctagaccttg
     5761 tgcttctttt tctgcttgtt cttcaggtgt caagttcgca catgttcctg cgtaatgagg
     5821 catttctcct cttgcgcgtt gtgcttcccg ttctgcttct aattcttctt ctgtgcaata
     5881 gcatttgtat gctcgattgc tagcaagcag ctgatcaatc agcggttgat agatttcttt
     5941 acgttcagat tgacggtaag ggccgtattc tccaggattt tcaggagatt catcccaatt
     6001 cattcccaac caagcaaggt tttccaactg gcttttttcg ccatcttcga tattacgttt
     6061 ttggtcagta tcctcaatac gaataataaa gtctccatca ttgtggcggg caaacaaata
     6121 attgaataat gcagttcttg catttccaat atgcaggtgc ccagttggac ttggtgcata
     6181 gcgtacgcgt actttcgtca ttttcttcct cttctttcaa actagacttt gactaacagc
     6241 aaaaattccg tcagttacgg cttctcaaat atcatttatt taaagccact cagcacaaat
     6301 tttacctttt ctaaggtaaa tagtaaagcc aactggttga atttccttac cagttggctg
     6361 aatttctttt ttattttgat tgtttagtct ttttctctgt ctctttttta ttgtcgattc
     6421 ctcgtcctgc atgtgcaggt ttagcaaaga tcatgcgtcc agcagctgtt tgaagggcac
     6481 ttgttacaac gacttcgata cgttcattca tgtaatgctg tccatcttct acgacaacca
     6541 tcgtgccgtc atcaaggtag gctactcctt gttggcgttc tgtcccggct tttactacta
     6601 gaacattcat cgtttcacca gggatgacga ctggttttac ggcatttgcc aacgcattga
     6661 tattaagaac aggtacattt tggaattcag agactttatt caagttatag tcattggtta
     6721 caatgacacc atctaaaagc ttagccaatt tgatcaattt gctgtctact tcactgatat
     6781 cttcaaagtc tccatcgtac atttcgacag atataccctc ttctttttgc aaggcattta
     6841 ggatatctaa tccgcgacga ccacgcacac gtttcaaact atcccctgag tccgcgatgt
     6901 attgtagttc gtaaagaaca aaattaggga tcatcaaggt tccttctaag aatccagtct
     6961 ttgtaatatc ataaatccga ccgtcaatga tcacgcttgt atccaagatt ttatatttat
     7021 ggaaattttc tcctgatttt cgttctaaca gttgactctc aacttgacta gctgtttctt
     7081 gctcgatttt tttgcttcgt gaaccaaaaa ctttcttcca ttcttcgatt cgtgtcgttc
     7141 ccattctcaa tcccagatag cctaagatga tcatcagtaa aatcggcaat acactattga
     7201 cgaatggaat ttccatattg taaagagggg tcgagatcac aacacctaaa gtcaatccga
     7261 tgatcgcacc gatcgcaccg aagagaagat aagtcaagct gacctcactt agtttttgtt
     7321 ctacttgttt gataatagct gagatatgtt tggccaacag caaagataat aaaaagaaaa
     7381 taagtgcgcc taacaggctg ttggtaaatg tattattcag ccatccgttt gactgttgac
     7441 cgattgcctg ccaagctgag ggtaaaaatg aaatacccaa gcttgcgccg acaatgatca
     7501 tcagcaaagt aatgacacgt ttttgcatcg tttttcctcc gtttcgacaa tcatttatta
     7561 gttagcggaa cacacgcttc aacgtttcag ccaaagttgc tactccaact atctcgattc
     7621 cttttggcgc ttcccagtta cctaagttgt ttttaggtag gtatactttt gtgaagccga
     7681 gtttttggac ttctttgact cgttgttcaa tatgactgac acgtctgatt tctccagtca
     7741 gtccaatttc accgatgaaa cattccgttg gctgtgttcc tttttcttta tagcttgaag
     7801 caatactgac agcgatagct aaatcaatcg ctggttcatt taattttacc ccacctgcag
     7861 cttttaagta agcatcttga ttctgaagaa gcaacccagc tcttttttcc agcactgcca
     7921 tgatcagaga tacccgattg aaatcaagac ctgttgttgt tcgtttggca tttccgaaca
     7981 tagtaggtgt gactaatgct tggatctcta ccaaaattgg acgtgtccct tccattgcca
     8041 caacgatagc agagccagtt gcaccgtcta ggcgttcttc taagaaaatc tgtgaaggat
     8101 tagcgacttc ttccaatcca tgctcatgca tttcaaaaat cccaatctca ttcgttgagc
     8161 caaatcggtt ttttactgct cgcaaaatcc gaaaactatg atgttgttca ccttcaaaat
     8221 ataacaccgt atccaccata tgttctaaca ttcttgggcc ggcgatcgag ccttctttgg
     8281 taacatgacc gacgataaaa atggctatcc catttgtttt agcggttttg agtagttctg
     8341 cagtggtttc tcttacttgg ctgacacttc ctgccacact tgtgatgtct ggctgcgtca
     8401 tcgtttggat ggaatcaatg ataacataat caggggagat atgttcgatg gcacgactga
     8461 tttcgttcat atctgtttca gcgtatagat aaaattctgt gttgatactt ccgaggcgtt
     8521 ccgcgcgtaa ttttatctgc tctgcgcttt cttcgccgga tacatataag acttttccgc
     8581 ctatggctgc taattgctga gaaacttgta acagcaaagt ggatttcccg atacctggat
     8641 cgccaccaat caagaccata gaccctggca ccacacctcc gcctaacaca cggttcagtt
     8701 cttctaattt tgttttgatc cgaggttctt ttttagggac gacgtctgcg atttttgttg
     8761 gttttgcttt ttctcctgtc aaacttgtcc gagttctgcg gtccgttgta tcttgttcga
     8821 tttcttcgac catcgtattc cattttccgc aatttgggca gcgtcccaag aacttaggcg
     8881 acacataccc gcaattttgg caaacaaatt gtacttttga tttttttgcc attgtaccgc
     8941 ctccttttat cctaatcatg agacattaaa tcaagcttaa gtatctttaa cgttttattt
     9001 actggaagaa ccaaatccgc cttgacgttg agcagtcgtt tgatcttgat ctgcttttaa
     9061 aaacggcaaa aagatccctt ggccgatacg ctcgcctttt ttgatcacta catccttgaa
     9121 accaaagttg gtgaattgga acatgatatg gccttcattt gcctcattat tgtaataatc
     9181 actgtcaatc acacctactc cgtttgtcaa cactaaaaac cgcttcaacg gattgcttga
     9241 acggttagca agctgcaaaa attcatcttc tcccatgtaa gctttgattc cagtaggcac
     9301 gagggttggt ttcaattgtt tttcattttc tgatacatct tttttcaatc catctgcgac
     9361 tgtttccaac ccctttttcc agcctgcttt ccagatactt ggaatcacga cctcttctgc
     9421 tgcttcaaaa tcatagccag cagcatgatg tgtagcgcgc tgcggtagat tgatattttg
     9481 atctttatat ttagaaataa cttcaaatcc tctttttctc accggttttc ctccctaaat
     9541 aaactactcc tattctaaca tatccgaggc tgtaaaagac tttctttaca aattcttcaa
     9601 gaaattctgc tatcttaaag taaaccatta attcgttctt tttccaaacg actgcaagtt
     9661 gtacaaaaaa actatttttt acatgacttt actcgtcaaa acaagtagaa tagaacgaaa
     9721 ttaatgagtg gatatttgat tgaggtgtca catgcgtaaa gaaattaaaa atgcaaaacg
     9781 aattattgtc aaagtaggaa caagtacatt gatttatccc aatggaaata tcaatcttgc
     9841 ggcaatcgat cagttagctt ttgtcctttc tgatcttaga aatcggggaa aggaagtgat
     9901 cttagtctca tcaggagctg taggcgttgg tatgcataaa ttacatttgt cagaacgtcc
     9961 gactttgatt cctgaacagc aagctgcggc ggctgttggt caagccgaac tgatgaatat
    10021 ctataatcaa agattcagtg tatataatca acagacggca cagatcctcg tgacaagaga
    10081 cgtggtagat tatcctgaaa gtcgtaaaaa tgtgaggaat acattggaac agcttttgat
    10141 tatggacacg atccctatcg tgaacgaaaa cgatactgta gcagtggatg aattagatca
    10201 tctgacaaaa tttggcgata atgatcagct gtcagccatc atggcaaaac ttgtcgcggc
    10261 agaccttttg atcgtccttt ctgatatcga cgggttttat tcagataatc cgactacaaa
    10321 tcccgaggct cagatgtatc gctatatcga taaaatcgat gaaaagattc tggcacaagc
    10381 aggtggtgct ggtagcacat ttggaacagg cggtatgcag agtaaattaa aagccgcttc
    10441 taggatattt gaagcaaaca gcgcaatgat tttagcaaat ggaaaaaatc cggcaattat
    10501 ttttgatatt cttgcaggaa aagaagtagg tactttattt aaggaggact tgaaatgact
    10561 gacttgatgc aattaggaca acaggcaaaa gatagcgcgc aacaactagc acttatgcct
    10621 acaaaccgca aaaatgagct acttgttcaa atggcacaaa ccatcaaaaa gaatcaacaa
    10681 gcgatcattg aagcaaatca taaagactta gaaaaagcag tcgaaaacaa tatcagtgaa
    10741 acgatgcaag accgcttgca gctgacacct gagcgaatca atgccatggc tacagagatc
    10801 gaaaaaattg cttcgttaga cgatcctatt ggaaaagtag atgagatgtg gacgaacacc
    10861 gacggattga gaatcggcaa aaagcgcgtg ccactaggcg tgatcggcat catttacgaa
    10921 tctcgtccaa atgtcacgac agatgcagct agtctagcat tcaaatcagg gaatgccgtt
    10981 atccttcgcg gtggcaaaga agcattcttc tcgaatcaat tattagtcca attattgcag
    11041 caagtactgg tcagtgctgg cgaatctcct tatgccatcc agtttgtcga tgatacttct
    11101 catgaaactg caggacagtt gatgcgattg acagaatact tagatgtgct gattcctcga
    11161 ggcggagcaa aattaatcca acgagtcaaa gaacaggcaa cggtacctat cattgaaaca
    11221 ggtacaggaa ataatcacgt atatatcgat aaagaagcac agttatctat ggcagtgaac
    11281 atcatcgtca atgcaaaagc ttcccgtcca tctgtctgta acgcggcaga aacattgctg
    11341 atccattctg agatcgcacc ttttttcttg ccagctattg aaaaagaact ggtagaacac
    11401 ggggtctctt tacgtgcgga tgctcgtgcg ttagaatatc tagagacggc tgctttagca
    11461 gaagacacag attgggatac tgaatatcta gattatatcc ttgctgtcaa agtggtcgat
    11521 tctttggatg aagcaattgc acatatcaat cggcataata ctaaacattc agaaacgatc
    11581 gtgacagata gttatgcggc tagtcaacgt ttcttaaatg aagtggacgc ggcggctgtc
    11641 tatgtcaatg cctccactcg ttttacagac ggatcggtat ttggttttgg tgcagagatt
    11701 ggtatctcta ctcaaaagtt gcatgcccgt ggtccaatgg ggttaaatga attaacttcc
    11761 acaaaatata tcatttatgg cgatggacaa atccgcgaat aatgtttcgt gtgaaacatt
    11821 attcccgcct tttgcctttt ttctgtaaag gttctatcca actagaaagg tgtcaatatg
    11881 tttgaaattt taatgatttt ttttatttac cttatttctc tgaatatcgc tgcttttctg
    11941 ggagtcggta ttttatccct tttctttcaa ttcaaaaaac gaagcatagg gagtcaacgg
    12001 gaaaaatggt cgcagtattt tgacaaaatc gggcaaaaag ggctggttac acgcttgcac
    12061 attagttaca tggttgcatt atgcttatta gcgacaatca attactattc ttttttcgat
    12121 cattcaatcg cttatacgat tacgctactg attgctggga tttttcactt atcttataaa
    12181 tatcagctga ataaaaacca cttgaatcgg actttccgct aaaaaatatc cgtacgataa
    12241 gtagagtgcc ttgaagatcg gcaaaaaact gccggacttt ttagaggctg tgacaaagat
    12301 tttgtcacag cctctttttc catataaacg gcgttcgcct accagctgcc tggtaaatct
    12361 gtttcctctt cgttaagaag cgaaaatccg tcaaatgttt cgccatcata ataacgaagc
    12421 agctcatcca tggacaagtt catcgttttt tctttttctt cttccgctga ttcagccagt
    12481 agctgttccc gatacacctg atacatacca gctatttttt gctgcatcgc ttttctagtt
    12541 tcaccttccg cattgattat tggcaattct ttgatatagc cacgataaat caaccgatca
    12601 tctgaaaatt caatgggtaa ttcaacgagt gtcaacccga cttcctgaaa atattcttcc
    12661 agcatctatt atccccccat tctttttctt aaatgaattt tagcttacct actgaaaatt
    12721 ttccagtttc attattttga gaaaaaaagg gaatgtgaca aaaatcgtgt cacatcccct
    12781 ttttccgaat aaatggtgtt cttctgccaa ctcctcaatc ataagtcaca atattcaaca
    12841 aacatgagaa actgtttttt gaaattgttc cttgtgattc agagttaaac gacagtctca
    12901 caacctcttg gtaaacggcg ttcaatcagc tgaccctcgg tattagctca aaaaacggta
    12961 aaatatgaga agctattttt ccgttttgtt tctctaatac ttaggataaa acgctgattt
    13021 tacaacctct gttaaacggt gttcaatcag ctgtccttcg gtattagctc aaaaaacggc
    13081 aaaatatgag gaactatttt tccgtttgtt tctctaatac tcaggacaaa acgctgattt
    13141 tacaacctcc tgataaacgg tgttcaaaag cttgcatctg cggtaataag cccaaccaaa
    13201 cgaaaaatat gagaagctat ttttgtttgg ctgttcttat gacttgatgc aggacagctt
    13261 tttcacaacc tctgatttct tattcttctt catttgtagc aaaataatta cggtgataaa
    13321 tgatacaaca tacactgata agaacaatgg cacctgcaaa ataaaacgca tagctttcac
    13381 caatatgttc accaatcaca cctgcaataa atggtgctaa agctccaccg aaaaagcgga
    13441 caaaactata gctggacgaa gcaacgtttc gttgcagacc tggtatttcc atagcaatcg
    13501 tcgtcaacag tgtattgata attccttgga aaaatcctgc taacacaatg ctgatcggta
    13561 caaggactgg catagaagtt ccagctccta ataacagcaa acaaataatg aataagacaa
    13621 atgctgctaa aattgtccga taagtcgtaa acttccgctc aagagccggt gcaacgaaaa
    13681 tcgaagcaat agccagaagc acaccccagc caaaaaatac aaaaccgact tctaattcag
    13741 aatagccaat caataaaaat ggcgcatacg caagtaaagt aaagaatccg aaattatata
    13801 acaatgcgat tagtccaact gaacgaagtt tatgattgct taacgctttg acaccttccc
    13861 aaaggcctac ttttctcttt ggtttctcag tttcaggtaa aagtaccgct agggctataa
    13921 aaccgcaaaa catcaaaaat gctaccccaa aaaatggata acgccatgaa ccactgccca
    13981 agaccccacc gactagaggg ccaacagcca ttccgcagcc aagtgctgct tcatacatca
    14041 tgattgcttt ttctgtgtta ccaactaaaa tactgacgat cgttgctaaa gcagtagaga
    14101 caaataaagc attcccgaca ccccagccgg cacgtaaccc aatcagcatt ccgatattcc
    14161 cagataatcc gccaagacca gcgaatacaa cgatgataaa caatcctgtc atcaatgttt
    14221 ttttcaaacc gatccgacta gataagaaac ctgtaaatag catcaccaca ccagtgacta
    14281 acatgtaact agtaaataac agggtcgttt cagctggcgt agcgtttaac tgtgcagcaa
    14341 tcgtttttaa gattgggtct actagaccca ctcccataaa agcgatcata caggtagctg
    14401 ctacaactag cgctgaactt ttcccctttg tttcttcatt tgtttgttct tgtatatcta
    14461 tttgattctc caatatttcc atccctttct ctctattagc atcatacatg atttatttta
    14521 catgtcaata tgcacatgta aaacgtggaa actttactgt gatataataa agaaaataag
    14581 acgagaggta ggatttttat gaatacttta gcctatatat tattatgcgt gcttttatcc
    14641 aaaccacgtt ctgggtatga attgaagcag ctgatcacaa ttttttggga agcccatcac
    14701 agtcagatct atacaacgct tgccaagtta gaccagcagg gttatgtgac cgttttggac
    14761 aaagacgagc actctcaaaa aaagatctac cacttgactg atgaaggacg tgctcttgta
    14821 gaggaatgga tcaaagagga aactcccgct cctacgcaaa aagatgaatt tctagcaaaa
    14881 atctatgctt tttcgacgct cgataaaaca acagctactg gactgctatt cactagagaa
    14941 caacagctga acaaaatcat gcaaaaaaat caaaaaaaat tgaatgacct ctcgctagac
    15001 aagaaacaag aatttggacg ttatgttgtg atccagcgca gaattatgct ttgtcagcag
    15061 gaactccaat ggtgtcagca agtaagagaa caaatgcagc agttttttga gtaagggata
    15121 aacagaacct ttctagaaat atctattgaa gagcaaatta tcttcaataa aagagcaata
    15181 cagttggaaa agaaaaagcg atccgaacga caggccaaaa cctatcgttc ggatcactat
    15241 ttttctagaa ctgcattcaa gagactatga caaaaagcat gccaaggcct tttttcaaat
    15301 aacagtgttc catcattcat tactttgaat tgcttttagc acacttcttg aaagcgggcc
    15361 aacgatgatc aactgcaacg gtaaagcggc aatcacattc attttccaag cattcatata
    15421 agcagagaat aattgatcag gtattccttg ttccatgatc acaccgaata aagacataca
    15481 tgtcaccatt cctaaaacca tcaagcatga aatagttaag atcatttgga ccttgctttc
    15541 tggatttact ggtagtttaa aggcaatttt tttcgccagc acaccgatga tgaagacatc
    15601 caaaatgaaa gccaccacga atccaggcac tagaccaatc aacaaacttg ataaatgaag
    15661 atcttgatgc agccataaat tatacgcgct catccccaag accatcaaaa aacacatcat
    15721 tgttgtaaaa attattcctt cttttttatt tgtaggcaat ctgtaccact cctttaaatt
    15781 tgtcacgaaa atcatcgtag cacatttaaa aatttctcgt aagtactttt tttattttct
    15841 gcccttttat ttcataaact tcatcagccg tacgctcgac aaagtaagaa tcatgagaag
    15901 taaacaaaat cgttcccgga tatatttgca ttagcttctc taaagcttcg atcgttgcca
    15961 tatcaataaa attagtcggt tcatctaaca gcaacacatt acttggctta gtaaaaagca
    16021 aagcaatcgt cagacgggtc gcttctcccc cacttaatgt acataacggt ttcgtcaaag
    16081 tttcctgagc aaaccctaag tgattcagca aactacggac aagcgattct gaaaactctg
    16141 tttgtctcat gacaaaacga atgatggatt cttcagatgt catttgataa cccttttgct
    16201 gatatacttg aaagcttact ttcggagaga gaaggattcc ttctccttgt ttgcggatat
    16261 gttctaataa ggttgtctta cctgaaccat tttcaccgac aatcgcgatt tttttaccta
    16321 aagaaaactg aaaatcacca tttaccagta atgttctccc acttctttcc aattggacat
    16381 tttgtcccat gatgggatag cggctgtgga gttcaagaga ttttggtact ggaaaacgga
    16441 tttgtttcat ctgctgcggc ttggttgttt ctcctatttt ttggagtctt ctctctaatg
    16501 ttttcgcatt cttttggatg gctttttgta cactgtcttt ttgtttagag gaagacaacc
    16561 gatccagtct gatttgttgc tttttctttt tactggacac tttttccaac ttttccgctt
    16621 gttttttctt ttcttggatc gatttttcca atcgtttttt ctctttcatg atcgactctg
    16681 cttctcgtag ctgagtcttt ttctccaatt ccttttgagt gagataggca ctataattcc
    16741 ccgaaaactc tcggatttct ccgtctttta cttcccagat tttcgatgct agctcatcta
    16801 gaaaatagcg atcatgacta acaaaaacca gcgtaccata ataataacga agctcttcaa
    16861 tcagtcgatc aatacttttt ctatctagat gggttgttgg ctcgtctaga agcatccctt
    16921 gttcataaga agaaagaaga cgtatcaact cgatctttcg ttcttcccct ccgctccatt
    16981 tccgctctga agtttcttcc agtcttaact ggaaatgact ggctaattcc aaatttaggt
    17041 cctttgcttc agcgagatag gttaattgag gcaggtagtt gaaggtgatc tctgtttgga
    17101 ttttcccatg atcgggaaaa agttcaccgg ctattagttt gagcaaagtc gatttaccga
    17161 caccattttc tcctatgatc cctatacgat cttgctgata gacacgaaga gaatcaatag
    17221 atagttctag ctggttgcca aagctgactt gtagatttgt tatatttact gctaaatttt
    17281 ccatatgttt tacactccta tttcatgtaa aacaacaatt atcgatacta aaaaagagca
    17341 gaaaaatctg tttgcaaaaa aactgcatca catcttttcc tactctgaac gaaataacta
    17401 tgactaaatc accaaatctg tcatagcttt cacctattct actgtcgttc gaaaaaatgg
    17461 acatactcca tccatgaaat tgttgtttat acatgtcttt tgaatttatt tagttgttat
    17521 tcaaaagttc gaaacgtaat ttcatcgatg cagtcatttt tccgacctcc ttttttatta
    17581 ggttaccaca cacgattcaa aaaaagcaat agctactgaa tagtaactga aaacctttta
    17641 ttcgaacata gtgatctttt ctggtgctaa acgaacagac tcgtaacctt cttctaccgc
    17701 ttttcctatt gataaaataa taactggaac ataacggtct ttatccaaac caaatgcctc
    17761 tgccaactga tctgcttcga atccaccaat tggatttgtt tcatagccat gatcacgcgc
    17821 aactaacatg aattgcatag ccgctaagct agaatcgact ttgactacat cgttcatttc
    17881 ttctcttgaa aaactcttgt aataaggaat gatcgcagct aattgttgat ctctgacttc
    17941 ttttggcatc ttccctgatt cgtaagcttg gttgtagatt tcttcgccat attcgtaaca
    18001 ttgcatatcg ccaaaaatca tgaccattgc agaagaagta tcattttgtc tcgtattgaa
    18061 acggatcaaa ggacgcaaga tttcttttgc ttcctcgctt tcagcgacca caaagcgcca
    18121 tggtttcata ttgacagaag aaggtgcagt cgtcgcttct ttgatcattt ccagcatttc
    18181 ttcgtgtggg attttgtatg tttcatcata tacgcgaacg gatcttctac cagtcataat
    18241 tgttgaaaaa tcattttttt gttccattat tattaacctc tttcgtttct gttgttatat
    18301 tgcaaagttg ggtattcaat ttatctaaaa gtgttagaaa ttgtttcatt tctacatcgc
    18361 tgaaagccgg ggatagcacg gaacctgtat ctttatggtc catttcacaa tgcttgattt
    18421 tcttctgggc tttttctgtt agcgctacat acacttcccg gttgttctga ggatttctct
    18481 gacgcgaaac atagccattt gcttccaata ccttcagatg acgagtaact gctccttgat
    18541 caatctgcaa cttttcttgc aaactgcttt gtagcaatgg attttcttct ttcagtctca
    18601 tcatgatctc gtatctagta agacttatac cgatattttc ttcaaagatc tggacgattt
    18661 tttgatcggc taatttgatt tgatataaaa ggtgactaat ctcttctaaa gacataattt
    18721 cctcctttct aataattgac tcgtcaacga ttgacttgtc aataatagaa taaaaaaaga
    18781 aaaaagtcaa agaatcagct gtgtaataaa aaaaacgctc tcaatagaaa agtatagaga
    18841 aaagaaattt tttgtagaat gaacggaaaa aatgataaag aaagcacaac gctaaaacca
    18901 agattattga ttcttatcaa actatagaag atagctcttc tgcagtagat aattttacac
    18961 cgccaaaaat ttccgaaatc gccttttgca agaatcccgt cgaatattag aaattggatc
    19021 agtatgctta aacagtaata aaaaagttat gacaagatgt tgtcacaacc ttttttgagt
    19081 aaacggtgtt caaaaacttg catctgcggt aataagttca accaaacaaa aaacatgaaa
    19141 atctgttttt gtttggctgt tcttactact tgatgcaggg cagctttttc actacctctt
    19201 tttaataacc ataaaatcct attccttcat atctccagcc catcggtgtt agccaatctc
    19261 tttccttagc actcaaggta tagtgatggg taccagtttt ttcatttgga ttaaatagtc
    19321 gataaattgg cttggaacca ccagaataaa aacttagacc ttcataacgc caaccgattt
    19381 ttgttaaaga gttgatctca gattgtgcca ttgtgtaatg atggtctcca ctgtaaggat
    19441 tgtataaacg gtagacaggt tgtccgctgt ttggtgcttg ccaagcaatc ccttcatatt
    19501 cccagcctac agttggcaga taattttttt ctccggcatt caatgtatat aaatgttctc
    19561 ctgtatgacg gttatacaga cgatacatcg gagaagttga agttggtgct gtatcatact
    19621 gtgtcagatt ataggtttcg attacattgt tcaatgtaga accataatta ggcgcagtgg
    19681 cataacgacc tgttagccaa gccgtagcat ctttgtaaga gttcgtattg cttttccatg
    19741 caccggaata gtagtaaacg cctggctgaa aagaagtcgt cttcaatacc gttacgttat
    19801 cttgcaatga ttctttgtac gaaggatatt ttctaaaagc ggcattcacc gtaatccatt
    19861 gcccattcac atattccgaa gtaggcatcg taacagattg gccattatag cttcctttaa
    19921 tcccaaatag attgtaatta ggggcagaag ccaaagtact gttcccccag ccactttcaa
    19981 cgatggcttg agcaatcatg actgatgcat ataagtcatt ggcacttgcc aaactttgtg
    20041 cagttgaggc aatactatca atgaacgctt gttgagaatt agcagatgag cgagcataaa
    20101 aagcatagcc agtatagttt tcgaattcag acaaatctgc ttgatgggta tgtgtcaatg
    20161 tttgcccatc tgtcaattgg ttcgtgattt cttctgcatt ctctggattg ttctgagctt
    20221 ctgctgatgt actgtcttcg gtttgtgttt gttctgttgt ttcagttgtt ggttcttcgg
    20281 ttgaaattgt attttctgag acagtagctg actgcaaagt actttctgta tcgttttcaa
    20341 ttcctgtttc tgaagaactc gatgtctgag ttgcgacagt ttctgtcgat tcttcagcta
    20401 gcactgttgt gttggcaact gctagatttc ctaaaagaat aacgctaaat aataaattct
    20461 ttgctttcat cttttttctc ctttgaatta tgaaactttt cctaaataac agtgtaacta
    20521 aaaaagtaaa agcaagtcaa tgtccatatt taaataacat ttgtttcttt ttaataaatt
    20581 tgctttttct gcataaaaag acgtaaatga agaatattct tctccattca cgccttaaat
    20641 tgatggtcgt ttgaataact atttaacaag taattctctc tggatattat ccgttatgga
    20701 ttatacttcc tgtgtcgtcg gacggataat catgttgctg acagacatcc ggtctggcgt
    20761 ttctatcgca aatgctacag cagaagctat atcttcagat gttaatcccc attctttttg
    20821 cgcttcatga agttcaagag cagccttttt gtcgctgatg gtttgatata attcagtttg
    20881 aacagctcct ggagagatga tcgtagattt gatgttattt tgccgttgct cttgacgtaa
    20941 tccttccatg attgcacgga cagcaaattt tgttccgcaa taaacggcgg aatcaggata
    21001 aacaacatgt cccgccacag aatcagttga aatgatatgc ccagattttt gtttttccat
    21061 gattggaagt acggcggaaa ttccatttaa cacacccatg atgttgatat caagcatatt
    21121 ttgccattca tcacggtgac cttcaactaa tggagcggtt ggcatgattc ctgcattatt
    21181 gtacaataca tcgattcgac cgaacttttc catcgctaaa tcgataactg cttggacttg
    21241 tgcaaaatct cttacatctg ctgtttgaat atatagctca gcttctggta aagattcttt
    21301 tattgctttt aggcgatctt ctcggcgtgc agctataact agtttagccc ctttttctgc
    21361 taatagcttc gttgttgctt caccgattcc gcttgaagcg cccatgatga caatcacttt
    21421 ttctgataaa tcattcattc tattctcctc ctgttttgaa aaatctcttg cttatactca
    21481 ctatctctta tttcatctta ctgttcaaat aatccgttcg acaggtttga cgattttggc
    21541 agggattcct gcaacgatgg tattgtctgg gacatctttc gtaacagttg cattcgctgc
    21601 aacaatggca ttctcgccaa tcgttacgcc tggcagaata gttgctcctg ctccgatcca
    21661 agcatttttc ttgatgtgga ttggctttac tgacaagcct cttcgttctt ttggatcggt
    21721 tagatggttc accgtgatga ggttgacacg aggaccaatc aatactgaat cttctaaaca
    21781 gatgccacct aagtcaacga acatcacttg acgattgata aagacatttt tccctatact
    21841 aatatgtttg ccgaaatctg tataaaaagg cagcagaaca gtcaccgtat catctacttt
    21901 gtcactaatg atattttcta gccgtttgcg gatatctgct ggttcatggt acccagtatt
    21961 cagcgctgct aaaggctctt cgttttccgc ttggatctga tggatcactt caaagagcgg
    22021 atccgttact cgaatctctt tattgacgat ttgtttttcg atgaggcttg tcattttgct
    22081 ccctcctttt atccttactt tataacggac ggcttatgat gtctaatact tataaaacat
    22141 acttaactat tccttttaac tatacttagt ggtatgcttt tttaaaaaca ggagggaaaa
    22201 aatggatatt cgtatactga cgtatttttt agcagtagct agagaaaaaa atatcagccg
    22261 agcagcaaaa tctctgcata tcacgcaacc tactctctca aaacaactga aagatttaga
    22321 agaagaactt ggtgtgacac ttttcactcg aggctcccga gagatacagt tgacggattc
    22381 tggtcgatat ctttattcaa aaggaaaaga aatcttgtcg ttggtagaaa aaacggcgaa
    22441 caatttaaca aatgacgcaa tcatctctgg agaaatctac attggcggag gcgaaactaa
    22501 agcaatctct ttcatttctg atagccttca ttcgttgatc aaggcgcatc ctgatatcca
    22561 gtttcattta tatagcggga atgcagatga tatcatagac aaactggata gcggattact
    22621 tgattttgga ctcgtaatcg aaccgacaaa caaacaaaaa taccagtcat ttcgactgcc
    22681 agcccaagat cgttggggac ttttgataca tcggaatcat cctttagcaa agcaaacagt
    22741 catccatccg caggaactga aaactgttcc tttgatcatt tcccggcaaa cgtcagtgga
    22801 cagtcaacta gctgaatggc taggtttcag catagatcag ttatcgatcg tcggaacata
    22861 taacttattg tacaatgctt ctttgatggt cgataacggg acttttggtg cattagctct
    22921 agatgggatc atcaatactt atgggacgga aaaagtattc atcccactcg atccgccatt
    22981 acaagcttca ctgaatctca tttggaaaaa agaccagcct ctttctcctg ctgctcgtaa
    23041 atttttagat gtcctaacgg agaaatacag ttaagtagag tttttttcct tcgctttcag
    23101 ctttttaaca acgaataagt taagcaaaag atccaatgat taaataacat aagtttctta
    23161 atttgttcct tccttttatt tctgataatt ccagtaaatt actataatta taaaaaaaga
    23221 attgacaaaa cataactgtc tatataaaat tgattacgtt aacaataatt cgtttcatgt
    23281 gactagatca tactaggcat gaaaaatctc tgaggaacat ggtttcccta ggaggttttt
    23341 cgtgtctatt tttgtttggc cgcaatcaac tggtctagca aacgtgaaaa ccaaatcttc
    23401 aaaaagggtg cgcgcatgaa aatcaaaaag atctttaatc aaaatgcagt attggtagat
    23461 gacggtggac aggaaaaagt agctattgga aaagggattg gatttgacag aaaaagaaat
    23521 gatttgattt ttgatcgtga tgtcgaacgg atatttgtca tggaaccaga aggtcaaatc
    23581 aagttgcaaa atttacttaa tcagattgat gaacgctttt tatttgcagc agaaacgatc
    23641 attgatcatg cggaaactgt tttgatggaa aagttgaatg aacatgtatt gatcgctttg
    23701 accgaccaca ttgctttttc tgcggagaac attaataacg gtatcgtcat ccgcaacaaa
    23761 cttttaagag aaatcgaagt gctttacagt gatgaattca gtattgctca atgggcagta
    23821 gagtatctga caaaagagct tgggattcca tatacttatg acgaagcagg atatatcgca
    23881 atccatattc acagcggacg aagcggtcgg aacaataacc atcgcagtat ccgtgaagtg
    23941 acgattgtat ctgacatcat ccatttagtg gaatcagaat tggaaacaga tatccattca
    24001 gaaccatttt cactgaatta ttcaagactg gtgaatcatt tacgactgct tctacaacga
    24061 acccatgcgc aacaatatgc tgttttagac acggaaatcg tagaaatggt caaacgaaaa
    24121 tacccagaaa gctataagat ttctaaaaaa atccgtgtgt tgctgaccaa agaataccag
    24181 ctcgcgatca cgaaagaaga actgggttac ttggccatcc atatcgagcg tttaagaagc
    24241 gcaatcgtac aaacaaatgt taacaatcat gaggaggaaa aaaattgaaa gcatatctac
    24301 aacgaatggg acgttccctt caacttccag ttgcggtctt acctgctgct gctttactag
    24361 tagggatcgg aaactggtgg gcatcttata gtaacgacat tgtcgctcat tttttacaag
    24421 ctggcggtaa cgcagtttta ggtcaattac ctttgctgtt tgccgttggg ttagcactag
    24481 ggatgtcagt ggacaaatca ggtgctgctg ccttggcagg tttggtcgca tttgaagttc
    24541 cagtaaatgt tttaaaaact gattcagtag ctactttatt gaatatcaaa ccagaatcag
    24601 ttgatccatc ttttgctcag atcagtaacg tatttattgg tattattgcc ggattgattg
    24661 cggcagctct ttataatcgt ttccacgaag caaaattacc gatggcttta tctttcttta
    24721 gcggcaagcg tctagtgcca atcttggctg cctttgtcat gttgctgatc agtgcagtgc
    24781 tactgctcgt atggcctccg gtttactctg ctttagtttc ttttgctaaa ttcattttga
    24841 gtttaggctg gggtggtgca ggtctttatg gcttcttcaa ccgtttactg atccctacag
    24901 gattgcatca agcaatgaac tccgtgttct ggtttgacgt agcaggaatc aatgatattg
    24961 gtaaattttt ggcttctgaa ggaacaaaag gcgtaacagg aatgtatcaa gctggatttt
    25021 tcccaatcat gatgttcggg cttccagcag gagcatatgc tatttacaga aatgcacgtc
    25081 cagaaaacaa agcaaaaaca gcttccttga tgttagcagc agcttttgct tcattcttta
    25141 ctggtgtaac agaaccactt gaattctcat ttatgttcgt tgcttggcca ttatatgtct
    25201 tgcatgcaat tttcactggg atttctttag cagtgagtgc attcttccat tggacagcag
    25261 gatttgcctt tagtgccgga tttgtcgatt acttcctatc tttgaaaaat ccgattgcga
    25321 atcagccatt gatgttgatc gtccaaggat tagtaacagc agtgatttac tactttggct
    25381 ttaactttgc tatcaaaaaa ttccatctga tgactcctgg ccgcgaggaa gcagatcttt
    25441 cagatgacga tactgcaacg actaattcag acaataaata tgcggcacaa gcaagcaaaa
    25501 tctacgctgc attaggcgga gccgacaatg taacttcgat cgataactgt actacacgcc
    25561 ttcgtttgca agtcaaagat acaggcctga tcgatcaaaa taaaatcaaa gccactggcg
    25621 tacctggtat gaagatcatc gacggaaaaa atgcgcaagt catcgtaggt acagaagtac
    25681 aattcgttgc agatgagatg gcaaaactac atggtggtgc tgcaacacga cctgcacaaa
    25741 caaacaccgt ggttaaaaca gaaacatttg ccggtggaac gatcgaacaa gatatttacg
    25801 ctatcgcaaa tgggaaactg attccaatca ctgaagtttc agatgatgta ttcgcggaaa
    25861 aaatgatggg tgacggctat gctgtattac cagaaaatgg agaaatcttc tctccaatcg
    25921 ctggtacgat cacaaacatt ttcccaacga aacatgctgt cggaatccaa acagatgctg
    25981 gtatcgaagt cttgcttcat atgggcatca acactgttga cttaaaaggc gaaccattca
    26041 ctttatatgt aaaagaaggt caaaaagtcg caagaggtca attgattgct ttagttgatc
    26101 ttgccgctat tcaatctgca ggcaaaaaca cggatatggt cgttgtattc acaaatggcg
    26161 acaaagtcca aagcttagaa atcgaacccg cacgtgatgt aaaagcaaat gataaaatcg
    26221 gctctgtttc caacaaagcc tgagccatcg tcttccccag acttttttat cgataaacaa
    26281 acaggagctg agacaaaaat cttgtctcag ctcctttttc cgaataaaaa cggcgttctc
    26341 ctgccaactc cgcggtaata agtccagcca aacgtaacat atgaagagct attttttatt
    26401 tgactcttct tattacttga tgcagaacag cttttttaca acctctgata aacggtgttc
    26461 aaaaaccagt ttatcggtat gaagtcaaat ttatgcaaaa atgtggaaag ccattttcac
    26521 aaaatttttc ttcatactca aaactaatcg gctttttcac aacctcttta tacattattc
    26581 ctgatccaat atttctttta atgctgcttc aagagaagtt aatggttttc ccaatacttt
    26641 ttctaaatca tcagaggaaa tagctaactg atccttttta atatcatgtt ggatcgctaa
    26701 aaacatgtca gctgcttctt gagggaatcc ggcttctttt agattttcga caaacgctgc
    26761 atcacttgct tcaataattt ctaacttttt acctgtcgca cgctctaatg ccttggctaa
    26821 ttcttcatag tcagttagat tgccggataa ttctaagatt tctggagagt cactttctac
    26881 tagtactttt gctgcagctt ctgcatattc acgtttcaat gcccaacctg tttttccatt
    26941 tcctgcagca taaacgaatt gaccagtttt taaagcctgc ccaatgatag gtaattcatt
    27001 ctctacatac caattattcc gtaaaaatgt atgggaaata cctgaatcct taatcatttt
    27061 ttcagtgaat tggtgatctg gcgctaatac actgatcgag tgatcggcat ctgcaaaact
    27121 tgtataagcg atataagata ccccagctgc ttgtgctgcc ttcactacat tcttatgttc
    27181 ggcttgacga tttcctggaa cgccagagac aaaaagcaat cgatctattc ctttcaacgc
    27241 ttctttcatt gagtctaaat catcataatc accaatacgt atattaaatc cagcttcttt
    27301 cagtgcttct cctttttctt cactacgtac tagtacatag atttcatttt gtggaaccat
    27361 cttttttaag aattccagcg cataaccacc aaaacctcca gttgctcctg ttactaaata
    27421 atgcatattt gccactccct attcttttta tttgttaaga tttacagctg tacatttata
    27481 aattacacct ttatcataaa gagtgtggta agataagtca agcaaaaaaa atgaaaggat
    27541 gacaactgat gaagtattct ctgcaattta gtgatgcaat ccatgtactt gcctatattg
    27601 aaatgtttca agatacccga tcactttcta gtgagatgat tgccagtagt atcgaaacaa
    27661 atgcagcaaa tgtacgtaaa atcatgagtc agctcaaaaa atccggtttg atctttacaa
    27721 aaaccggaaa accgcagcct tcccttgcca aaagtccaga tcagatttct ttactagatg
    27781 tctatcgcag catcgaaggt aatacacagc ttatccaagt cgatccaaaa acaaatccaa
    27841 attgcgtcgt tggtgcgaat atccaagaag ctttagaaga aagctacgct cggctgcaga
    27901 aaaaggcaga agaagaaatg gctaagatca cacttgatag tctgatacat aaaatagccg
    27961 ttttggaaaa agaaaaaaga ccagaaaaca caggcctcgt agagagattt ttataattct
    28021 ctctactgtg cctgcatttt tctgatcgga atcgtctaca taaattcttt tattctccgg
    28081 gggacaaaag ccttgtcatg cccctttttc ctctctaatg ctcaggtcaa agcgctgaag
    28141 tcatgacgtc ttgataaacg gtgttcaagt agctgaccct cggcattagt tctgaaaaat
    28201 gcaaaatatg gaaagctatt tcccattttt cctctctaat gctcaggtca aagcgctact
    28261 ttcacgacct ctatattatc atggtttatt gactaccggt atccagattt ctcccatata
    28321 tttcccgttg gtaactccca tttcgactac agcatttgga cccccaacat aggcgtaatc
    28381 ttctacttct cttaaaacat gcccaaaggc cgtattcgtc aaatcatttc ttaattcttc
    28441 aggcgattct gctaaagcga ttacaacaag atacgttcct tttgggaatg ggatgattcg
    28501 ggtagtgttt ggaacatcct gactcgtttg aacaccgaca tagtacatca ttttcccgtt
    28561 atagggttcg ttgactgtaa agaaataatg attttcagca gcttgtttca atgcatctat
    28621 tgttccatta ccgataactt tttgctcaaa tgcttgtttc tctgcagcca aaccgacaaa
    28681 atccgtattg ctactcttca attccacacc aatacccgtc accgtaaaat ctggtttttc
    28741 ttcgatccta tactttgcca tttctcccac cctttctatt atattaagtt catcttacat
    28801 aaaattaaag ggaaatgaca cgattacgct cttttttcta gggatatttt tattttttaa
    28861 ctattttttt gctttcactg caatccaaat ctcactgtaa acattactga aatcctctcc
    28921 ccattttgta aaagagatct cgggagcttc gactaactca taatctgaag aaggaagcca
    28981 ttctgcatag attttccccc aagtatcttg taaagtttgg ggaaatggcc ctttattagg
    29041 aaaaatggcc caagtgtgtt cttctatagc aagcatttct aaatcagcgt acggattttc
    29101 ttttgtcgta gcaaagccaa tcatgtgcat caaactgccc ttttcttcca agcgggctcc
    29161 atcaaatcca taagaaacgt tgattacttg atttggatat aaatctccta acttatgcat
    29221 ctcttccctt tgagtctccg tgatcgtctg cgctaacttt tgtatttctt gattgactcc
    29281 ttcaaatttg acaggaactt ttttcgccac acctgccaca taaaactgtt ctttcttttc
    29341 gatacgaaat tccatcgatt ttcctcctct gacatcaata aaaaatgtca agcgtggaaa
    29401 agatttttgt atgccgtatc tgctgatttc tgaaggcaga tatcctgacc aatcacgaaa
    29461 agctcttgaa aaaccttcga ctgtttcata tccatatcta aacgccgttt ctgtcactct
    29521 tgatccatct aggagatcgc gattagccaa agccaatcta cggtttttga tatagtccat
    29581 tagcgacatc cctgcaataa acgtaaagat gcgttttaat tgatattcag agattcctgt
    29641 attttcggct attattttat aggaaagtcc ttctgtcaaa tgttcttcga tgtaatcgat
    29701 caattggttc agatctttaa gcatatttat cttcctcctt gcttgccttt ttatcatatt
    29761 atcaaagaaa gtcaattact tgatgaatca ggaaccaatt tagtcagatg tagaaaatgt
    29821 tgtatggatc taaagaaaat tctcaagcag cgtttcgtaa cggataattg gtattatctg
    29881 cctatctatg ttttaccttt agaaaaataa attttgggga agacacattc taagtaaaca
    29941 aagcggtaaa aatatctttt attcccttag ttaatccgct tactacctac tgtacaaagc
    30001 aaattgttgt tcaaactgct gatcagaacg ctgcttggat ttttctttgc gatacatcat
    30061 acctctttta tcaattggaa caattattta tttctaacaa aatcacttca tttttgtcag
    30121 ataaataaaa
//
LOCUS       DAYYCY010000016        27964 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000016 DAYYCY010000000
VERSION     DAYYCY010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27964
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.016"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(114..473)
                     /gene="rplT"
                     /locus_tag="ACHAZB_000581"
     CDS             complement(114..473)
                     /gene="rplT"
                     /locus_tag="ACHAZB_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFM8613281.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(526..726)
                     /gene="rpmI"
                     /locus_tag="ACHAZB_000582"
     CDS             complement(526..726)
                     /gene="rpmI"
                     /locus_tag="ACHAZB_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFM8613282.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(798..1322)
                     /gene="infC"
                     /locus_tag="ACHAZB_000583"
     CDS             complement(798..1322)
                     /gene="infC"
                     /locus_tag="ACHAZB_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFM8613283.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     misc_feature    complement(1366..1491)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            1642..2274
                     /locus_tag="ACHAZB_000584"
     CDS             1642..2274
                     /locus_tag="ACHAZB_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFM8613284.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(2276..3181)
                     /locus_tag="ACHAZB_000585"
     CDS             complement(2276..3181)
                     /locus_tag="ACHAZB_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFM8613285.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(3266..3925)
                     /locus_tag="ACHAZB_000586"
     CDS             complement(3266..3925)
                     /locus_tag="ACHAZB_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFM8613286.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            4241..5185
                     /gene="mvk"
                     /locus_tag="ACHAZB_000587"
     CDS             4241..5185
                     /gene="mvk"
                     /locus_tag="ACHAZB_000587"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HFM8613287.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            5178..6155
                     /gene="mvaD"
                     /locus_tag="ACHAZB_000588"
     CDS             5178..6155
                     /gene="mvaD"
                     /locus_tag="ACHAZB_000588"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HFM8613288.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            6168..7253
                     /locus_tag="ACHAZB_000589"
     CDS             6168..7253
                     /locus_tag="ACHAZB_000589"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004631 - phosphomevalonate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HFM8613289.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            7250..8314
                     /gene="fni"
                     /locus_tag="ACHAZB_000590"
     CDS             7250..8314
                     /gene="fni"
                     /locus_tag="ACHAZB_000590"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFM8613290.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(8563..9366)
                     /locus_tag="ACHAZB_000591"
     CDS             complement(8563..9366)
                     /locus_tag="ACHAZB_000591"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFM8613291.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(9386..10228)
                     /gene="kduI"
                     /locus_tag="ACHAZB_000592"
     CDS             complement(9386..10228)
                     /gene="kduI"
                     /locus_tag="ACHAZB_000592"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFM8613292.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(10244..11251)
                     /locus_tag="ACHAZB_000593"
     CDS             complement(10244..11251)
                     /locus_tag="ACHAZB_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFM8613293.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(11258..11902)
                     /locus_tag="ACHAZB_000594"
     CDS             complement(11258..11902)
                     /locus_tag="ACHAZB_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFM8613294.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(12002..12760)
                     /locus_tag="ACHAZB_000595"
     CDS             complement(12002..12760)
                     /locus_tag="ACHAZB_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFM8613295.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(12887..14038)
                     /gene="fucO"
                     /locus_tag="ACHAZB_000596"
     CDS             complement(12887..14038)
                     /gene="fucO"
                     /locus_tag="ACHAZB_000596"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008912 - lactaldehyde reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFM8613296.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(14120..14431)
                     /gene="rhaM"
                     /locus_tag="ACHAZB_000597"
     CDS             complement(14120..14431)
                     /gene="rhaM"
                     /locus_tag="ACHAZB_000597"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFM8613297.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(14433..15278)
                     /gene="rhaD"
                     /locus_tag="ACHAZB_000598"
     CDS             complement(14433..15278)
                     /gene="rhaD"
                     /locus_tag="ACHAZB_000598"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008994 - rhamnulose-1-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFM8613298.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(15292..16548)
                     /gene="rhaA"
                     /locus_tag="ACHAZB_000599"
     CDS             complement(15292..16548)
                     /gene="rhaA"
                     /locus_tag="ACHAZB_000599"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFM8613299.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(16545..18005)
                     /gene="rhaB"
                     /locus_tag="ACHAZB_000600"
     CDS             complement(16545..18005)
                     /gene="rhaB"
                     /locus_tag="ACHAZB_000600"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFM8613300.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            18158..19027
                     /locus_tag="ACHAZB_000601"
     CDS             18158..19027
                     /locus_tag="ACHAZB_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFM8613301.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(19153..20052)
                     /locus_tag="ACHAZB_000602"
     CDS             complement(19153..20052)
                     /locus_tag="ACHAZB_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8613302.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(20217..21041)
                     /locus_tag="ACHAZB_000603"
     CDS             complement(20217..21041)
                     /locus_tag="ACHAZB_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFM8613303.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(21052..22347)
                     /locus_tag="ACHAZB_000604"
     CDS             complement(21052..22347)
                     /locus_tag="ACHAZB_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFM8613304.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(22363..22842)
                     /locus_tag="ACHAZB_000605"
     CDS             complement(22363..22842)
                     /locus_tag="ACHAZB_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFM8613305.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(22848..23876)
                     /locus_tag="ACHAZB_000606"
     CDS             complement(22848..23876)
                     /locus_tag="ACHAZB_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFM8613306.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(23893..24999)
                     /locus_tag="ACHAZB_000607"
     CDS             complement(23893..24999)
                     /locus_tag="ACHAZB_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFM8613307.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(25228..27825)
                     /gene="adhE"
                     /locus_tag="ACHAZB_000608"
                     /gene_synonym="adhC"
     CDS             complement(25228..27825)
                     /gene="adhE"
                     /locus_tag="ACHAZB_000608"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFM8613308.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
BASE COUNT         7886 a         6133 c         5043 g         8902 t
ORIGIN      
        1 tattttagac ttatacgtta ttgtcctgtt tcatttgatg gaggattgta aataaaaaaa
       61 gaggaaagaa cataactttt cgttatcttc tttcctcttt ataattgttt agcttattta
      121 gccaaagctt cttttgcttg gtcagccaat gcagtaaatg ctgctgcatc gttaacagcc
      181 aagtcagcta acattttacg gttgatgtcg atttcagcaa ctttcaaacc gtgcattaat
      241 tttgagtagc tcaagccatt catacgagct gctgcgttga tacgagcaat ccacaattta
      301 cggaagtcgc gtttcttttg acgacgatcg cgatatgcgt agttgtatga attcattact
      361 tgttcttttg ctgttttaaa taaagtgtgt tttgaaccgt agtaaccttt tgctaatttt
      421 agcactcttt tacgacgttt acgagttact gttccacctt taacacgtgc catgtataat
      481 tcctcctaat aaaaataaat cttcagattc tgttatttgt acttcttatt tcatttttgc
      541 tagttgttgg cgaatacgtt tgaagtcgcc actagaaacc atgcttgctt tacgcaattg
      601 acggcgttgt ttttttgttt tgccgtggaa acggtgactt gtaaacgcgc ggaagcgttt
      661 caatccgcct ttaccagtac gtttgaaacg ttttgctgat ccgcggtgtg ttttttgttt
      721 tggcatgact atatttcctc ctcaaaatct ttcgttatcc gactatcggc tgtccaacag
      781 ccagttagtc attcaaatta cttactgtcg tttttcggtg ccagcgtcaa gaacatgctg
      841 cgtccgtcca ttttcgcttt ttgttccact gttgcaatat ccgcagtttc ttcagctaag
      901 cgattcaaga ctttttgacc aatttcttta tgggtaatgg cacggccttt gaaacggata
      961 gaagctttca ctttatcgcc cttttccaag aacttacgtg cattacgaag ttttgtattg
     1021 aagtcattca agtcgatcgt aggacttaag cgaacttctt ttacattgat cactttttgt
     1081 tttttacgcg cttcgcggtc tttcttttgt tgttcgaaac ggtatttacc gtagtccatg
     1141 attcgcgcaa ctggtggttt cgcaccaggg gcaactaaca caagatccaa gtttgcttgt
     1201 tctgcaatat tcaatgcttc tgcttttgtc ttaactccta attgctcacc atctgaaccg
     1261 atcaatcgta actctcgtgc acgaatgccg tcgttaacca tcatatcctt tgctatggtc
     1321 attcacctcc aaatattatg agagaaagaa aagcttaccg aataaacggc aataaaaaaa
     1381 cgagttctct tacagaaccc gcgcgtactt cacctaatag aaccagtgaa aactatctag
     1441 cccagtgaca ttatcaacta aggcgagaag cgggtgcttc tgcttttatt tctcaaccat
     1501 catagtatag caaaaactaa tctttttgtc aatgtttttc atacagtttg tcttcctttt
     1561 ctgaaaatga ttagattagc agactttcta gcagtgattc ccgcttcttt gctatactaa
     1621 agaaaaacga ggtgaaccca catgaaaaaa atcgaaccta ttccaccttc tcttcctctt
     1681 cttacaaagg gagagtttta tttagaagat gaggagatct tctctggtct tctgatcaac
     1741 gggggtgacc aaagctatct cgatgccaaa aatatggtat taagagagag tcatatccaa
     1801 aaacttacga tgcaaaaaac agttttacgt cattttgaat gcagtaatgt catttttgaa
     1861 aaatgtgatt tttccaatct ggagtggatt ggtggcagtt tccaccaagt cgtgtttcat
     1921 caatgtaaat taacaggtac gaattttgcc gaaagttatc ttcgagactg tactttcact
     1981 gattgtattg cttcgatggc ttcctttagt agtacaaact tgaaagctgt ctccttcgat
     2041 cattgtcaat tagaagacag cgaattttat gaggtcactt ggaaaaacct ttttttgtct
     2101 gacaatcagt tgaccggttc gaactggttt cgaacttctc tgaagggatt agactttacg
     2161 actaatttct tcacagcaat cgctttatct caagattctt tggcgggact aatcgtcaac
     2221 caagagcaag cacttgtgat cgcccaagcc cttggtttgc agatcaaaga ctagatcaac
     2281 gaatccgccg ccacagtgca ataagcatcc aaatagataa gaccaaactg atgatcaagg
     2341 cgattcccca tccgaaaacg gaatgggtaa agggtaactg gagattcatt ccgaagaatc
     2401 ctgtcacaat agttggaacg gtcaagatca gcgaccagac cgtcaaaaat ttcatcgtgt
     2461 cattcaagtt gttattcagc aaattattat aagtgccaga gagctggtct aaaatctgcg
     2521 aagctaagtt cgtcatttca actgcttgtc gtgcttcgat cagcgcatcg tccaactctt
     2581 ctttttcctt ttctgacaac ttgcgataaa tagccaatgc cttgatttgt tctaaaagta
     2641 ctacattttg ttttgttcct gtaactaagt aaaccaaacc gatctctaaa tctgaaagtt
     2701 gcagcagatt cttgttcgtt gttttttctc ttaatttcag attaagccgc tgacgttcac
     2761 tattcacttc ctcgattaaa ggaaaaaagg aatcagaaat caaaaacaat gtacgaaaca
     2821 ataaactgta tacactcatg tctggcattc gttccaaaag cgatcgaatt agtcgaatca
     2881 cgtactcggt attcggcgtg gtaaatgtaa acaaaccgtc ttcttttaaa ataaaagcga
     2941 taggactagt ttcataatga ttatcgactt tttgctggtg cggaacattt gatacaagca
     3001 gtaacgcttc atcaaaagaa tcgtactcta cgcgcgcccg ttcattttta tctagagaat
     3061 aagtcagcat ctcgtcacta atgccatatt cttcttttaa gtttttaaca gctagcacat
     3121 cgttttgctg tacattgatc caaaaattac ggtcttgatt aaaatagtgt ttttcccgca
     3181 tactctatcc tcctgacatt tgtttccagt ataaagaaaa aagtcgctca aataaaggtt
     3241 taccctttat ttgagcaact gtaaatcaaa tggctacttt ggtttggcga atctctgcta
     3301 atttttcttc gatcattttc aagacttctt cttttgcttg aggatcttcc acaaaattat
     3361 agcggtctcc atcgatttgg atttttggac tttctttgta ttcttcatac cattgatcgt
     3421 aacgtctgtt caattctttg taataatcgt atagacttgg atcaaaggac aattgttcat
     3481 acgaacgacc acgtttttcg atacgctcca acatcgtatc aaaagataca cgaatatgaa
     3541 ctaataaatc cggatgtttt ttatgggcag catagggaag ttcttccatc atgttttcca
     3601 gcagttcatc atacacacga acttctgttt ctgttgctct tcctagatct gcatttagat
     3661 ggaaaagcag tgaatcttca taaatcgaac ggtccaatac attattgtca tctttcattg
     3721 cttgtttgat gcttgcaaaa cgtttattca aaaaataaat ttgcagcaaa aaagcatact
     3781 gttcagggtt tgcataaaat aagggcaaga cttcattatc atcaatcgat tcataaaatg
     3841 cctgactatc aaagtgttca gccatcattt ctgttaaact tgattttccg gcaccaattg
     3901 tacctgctaa aacgatcacg ctcatttacc tccagctcac ttcatcacta aatattgtgt
     3961 ttaactccaa cctttttaaa tataccccca atatctagta gtgtcaattg tatttttttc
     4021 tagtcaaaga agaaaaaaag tcaaaaaatt cttaagaaaa aagtgttttt tctttagaaa
     4081 acgtattctt agttttactt ctgagatggt tttagtaaaa taaaggagga atgttttttg
     4141 agacccttag tggacaaaaa aattcgtgaa gcagcgcaac tcgttttggg cgctttattt
     4201 tttgcttaaa aatcactttt acgtgagaag gagttagatt atggcaaact atggccaagg
     4261 agaatcaagc ggaaagatca tattgatggg cgagcacgcg gttgtttatg gagaaccagc
     4321 gattgccttt cctttctatg caacaaaagt caccgcattc cttgaagagc tggatgcaat
     4381 ggacgatcaa ctggtttctt cctactattc aggaaattta gccgaagctc ctcatgcatt
     4441 aaaaaatatc aaaaaattat tcattcactt aaaaaaacag catgacatcc aaaaaaactt
     4501 gcaactgacc attgaaagca cgattcctgc tgaacgtgga atgggatcaa gcgctgcagt
     4561 cgccacagca gtcactcgtg ctttttatga ttacttagca tttcctttgt ctcgtgaaat
     4621 actattagaa aatgtccagc tttcggaaaa aatcgcccac ggtaatccta gtggaatcga
     4681 tgcagccgct actagcagct tgcagccgat ttattttaca aaagggcatc ctttcgacta
     4741 cttttctttg aacatcgatg cttttttgat tgtcgctgat acaggaatca aaggacaaac
     4801 aagagaagcc gtcaaagatg tcgctcacct ctttgaaacc cagcctcatg aaactggaca
     4861 aatgatccaa aaattaggat acttgacgaa gcaagcaaaa caagcaatca ttgaaaattc
     4921 accagaaacg ttggcacaga caatggatga atcacaatca cttctggaaa agctgacaat
     4981 cagcaatgat tttcttaatc tattgatcca aacagcaaaa gataccgggg cattgggtgc
     5041 caaattaact ggcggtggac gcggtggctg tatgattgca ttagcacaaa caaaaacaaa
     5101 agcccaagaa atcagtcaag cacttgaaga tgcgggtgct gctgaaactt ggatacaagg
     5161 attaggagta catacctatg tttaaaggca aagcacgcgc atataccaac attgctctaa
     5221 tcaaatattg gggtaagaaa aatgaagaac tcatccttcc aatgaacaac agcctttcgt
     5281 taacattgga tgcattttat acagagacag aagtcatctt ttctgatagc tatatggtag
     5341 atgaatttta tctagatggc accttgcaag acgaaaaagc aacaaaaaaa gtcagtcagt
     5401 ttcttgacct ttttcgtaaa gaagccggcc tctcactaaa agcttcagta atcagtcaga
     5461 atttcgttcc taccgcagct ggattagcct cctctgccag cgggctagct gctttagcag
     5521 gcgcttgcaa tactgctctt aagcttggat tagatgatct ctctctttca agatttgctc
     5581 gacgcgggtc tggttcagct tgccgaagta ttttcggtgg tttcgtcgaa tgggaaaaag
     5641 gccatgacga cttaagttct tacgctaagc cagtcccttc cgattctttc gaagacgatt
     5701 tagcaatggt tttcgttttg atcaacgacc agaaaaaaga agtgtccagc agaaatggga
     5761 tgcgtcggac agtcgaaaca tccagttttt atcaaggctg gttagattcc gttgaagggg
     5821 atctatatca attgaaacaa gcaatcaaaa caaaagattt ccaacttctc ggagaaacga
     5881 tggaaagaaa cggactaaaa atgcatggaa cgactttagc tgcccagcct ccattcactt
     5941 actggtctcc tgacagctta aaagcgatgg atgcggtgcg acaattgaga aagcaaggca
     6001 ttccatgcta ctttacgatg gatgctggcc ctaatgtcaa agttttagtg gaaaacagcc
     6061 atttatctga ggtacaagaa acatttacca aattatttag caaagaacaa gtaattactg
     6121 cacatgctgg tccaggaatt gcgattattg aataaaggga gatttttatg attgaagtat
     6181 ctgcaccagg caaactctat attgccggag aatatgcagt tgttgaaaca ggccatccag
     6241 cagttatcgc tgcagtcgat caattcgtga cagtcactgt agaatctgca cgaaaagtcg
     6301 gaagtatcca atctgcacaa tatagtggga tgcctgtacg ttggacaaga cgcaacggag
     6361 aattagtttt agatattcgg gaaaatcctt ttcattatat ccttgctgct attcgcttga
     6421 ctgaaaagta tgcacaagaa aaaaacattc ttttatcctt ttatgatctg aaagtaacaa
     6481 gtgagttaga cagttcaaat ggccggaaat atggattggg ttctagcggt gccgtgaccg
     6541 ttgcaacggt caaagccttg aatgtttttt acgcgttgaa tttatctcag ttggagattt
     6601 tcaagattgc agcactagcc aatttagcag ttcaagataa tggttcctgc ggcgacatcg
     6661 ctgctagctg ttatggtggc tggatcgctt tctcaacctt cgaccatcct tggctccaag
     6721 aacaagaaac tcagcattct atcagtgagt tacttgccct ggattggcca ggtctatcca
     6781 ttgagccatt gattgctcct gaagatttac gtttattgat tggttggacg ggtagccctg
     6841 cctctacttc tgatttggtc gatcaagttc accgttcgag agaagataaa atggtggctt
     6901 atcagctttt cttaaaaaat agtacagaat gtgtcaacga aatgatcaaa gggtttaaag
     6961 aaaataatgt aacgttgatc caacagatga ttcgaaaaaa ccgacaatta ctgcatgatt
     7021 tatctgcaat cactggggtc gtcatcgaaa cgcctgcttt gaacaaattg tgtaatttag
     7081 ctgaacagta tgaaggagcc gcaaaatctt ctggtgcagg tgggggcgat tgcggaatcg
     7141 taattgttga ccagaaatct ggcattcttc ctttaatgag tgcatgggaa aaagcagaaa
     7201 tcactccact gccgttacat gtctatagcg atcaaagaaa ggaaaaccga tgaatcgaaa
     7261 agacgaacat gtttcactag ctaaagcctt tcatgataaa caaaaaaacg aatttgactt
     7321 cgtacggatc atccataacc ctttgccgca aatagcagtt tctgatgtcg atctaagtac
     7381 acaggcagtt gggtttacac taagcagccc attttatatc aatgcgatga ctggcggaag
     7441 cgaaaaaaca aaaaaaatca accaagatct tgcaatcgtc gcaagagaag cagatttgat
     7501 gattgccact ggttcagtca gcgctgcctt gaaagtccct tctttagccg atacttatac
     7561 gattatgcga caagaatatc ctcacgggaa aatcattgcc aacatcggtg cagggacttc
     7621 tgtcgaaaga gccaaagaag ctatccgatt gtttcacgca gatgccttac agattcattt
     7681 gaatgcacct caagagttgg ttatgcctga gggagaccgt gattttacca attggaaagt
     7741 acttattcaa gaaactcaaa cagccattga cgtccccctc atcgtaaaag aagtcggttt
     7801 tggcatgaca agagaaacgc tcaatgatct agctgcttta ggagttcata cagttgatat
     7861 cagcggtcga agcggaacaa gcttcaccca gatcgaaaac gcccgtcgct ccaagcggga
     7921 actgagttat ttagctgact gggggcaatc gacagtctct tcattgcttg aagcaaatga
     7981 agcagacact tctatggaaa tcctagcctc cggaggtatc cgcaatgcct atgacatatt
     8041 taaagccctt tgtctcggcg caaatgccgt gggtacttca ggaaccgtac tgacgcatct
     8101 gatgaatcac ggcgtagaag aaactattat actgatgaag caatggcaag aagagcttcg
     8161 tctgctttat actatggtcg gcgcaacgaa taccgcggcc ctacaccaac aatctctgat
     8221 cttttccgga ccagtgaagg attggtgcga agcaagaggg atcgacttag tgaagtatgg
     8281 aagacgcaca gaaaagagcg tgggacaaaa gtaaagaaca cttttgtctc acgctctaaa
     8341 cacgtacaaa cggcggaagt aaaagcaact ccttcggaaa taagctgaaa ttcacaaaaa
     8401 tttgaaaagc aattttcgtg aattccttct tatttcttgg agttaaatgc ttctgtccca
     8461 gcctcttttt tcgaataaat gatgtttaat cagctgtcct tcggtattcg ttcaatccgc
     8521 tgatttcaca acctctccac taaacagtgt actaaagctc ttttaaggtt gttttccgat
     8581 ataggcaagt atgcctccgt ccacatacaa gacatggccg ttaacgaaat cagatgcgct
     8641 agaagccaag aagacagatg gtccagccaa atcgattggc tcgccccagc gtgctgctgg
     8701 cgtacgtcca acgataaatt gatcgaatgg atgacgttca ccgttttctt gtgtttcgcg
     8761 taatggagca gtttgcggtg tagcaatata acctggtcca attccattac attggatatt
     8821 gtattgaccg tattcagaag cgatgttttt cgtcagcatt tttaatccgc cttttgccgc
     8881 agcataagca ctgaccgttt cgcgtcctaa ttcactcatc atggagcaga tattgatgat
     8941 tttaccgccg cctttttcga tcatatccgg aataactgct tttgaaacga tgaatggggc
     9001 atttaaatcc acgtcgatta cttggcggaa gtctgccgca tccatttcga tcatcggtgt
     9061 ccgtttgatg atccctgcat tgttgactag aatatcaact gaacctacat cttctttgat
     9121 ctgttgaacc atcgcttgaa ccgcttcttc atcggtcaca tcgcagacat aaccttttgc
     9181 atcgatgcct gcttctttat aatgcgctat accttcatca actgattctt gattcaagtt
     9241 attaaatacg atttttgctc ctgctgtagc taaagagcgt gcaatctcaa atccaatacc
     9301 atagacagca ccggtgacta atgctacttt tccatctaaa cgaaacatat ccatactaaa
     9361 ttccatcatt ttctctccct tatgcttatt ttaattgatc catttgcacc atatccatgt
     9421 ctgtataggt gatattttct ccgcacattg cccagatgaa tgagtaattg cttgtaccta
     9481 cacctgaatg gatcgaccag cttggcgaaa taatggcttg ttcattgtct acaactaaat
     9541 gttttgtttc atctggtttg cccatcatgt ggaacacacg tgtatcttca ttggcataat
     9601 caaagtagac ataagcttcc atacgacgtt catgtgtatg acaaggcatc gtattccaag
     9661 cgcttcctgg ttctaaaatc gtataaccca tttgtaattg acagctttcg catacatttg
     9721 gatggatgta ttggtagatt ttacgttcat tcagtgtcag gccttcacca gtctccattg
     9781 gttttacttg gtcgatactg atttttacat ttggatattt atgatgagct ggaacagaac
     9841 taatatagaa tttagctggg ttttcagcat cttgagaaga aaatacaaca tgtcgtgttt
     9901 cttttccgat atagtatccg tcttgttttt tcatttcttc tttttgccca tcgatctcga
     9961 tgaatcctgg tccaccgata ttgatcacac ctagttcacg acgctctaag aagtaatcaa
    10021 cacctagttc tttgtctaat gtgatttcta attctttttc agtaggtgtc actccaccaa
    10081 agatcattcg atcgttgtgc gtataagtca aactgatttt ccctggttca aatacctttt
    10141 ctactaaaaa ttcttttctc aattgttccg ttgaataatg acggatatct tctggactgt
    10201 gtgtataacg tgtttccatt tcttgcattt tcttctactc cttctatcga atgatttttc
    10261 cagagccgca tgctaaaaat tgtgcgactt cttcttgact aaattggttg cagtccccat
    10321 ggatcgtatg cttcaaggca gaagctgccg tagcaaaatc aatgatttct tgcggagaca
    10381 tattggataa aatcccatgg agcacgccac ctgcaaaagc atctccgcca cctactcgat
    10441 ccacgattgg ggtgattcta tggacttggg attccacata ttcatggttc atccacaatg
    10501 tccccttcaa ttcattttca cttgctgaat gaacggtacg cttggtcgaa taaaacacct
    10561 gaatattcgg gaacttttcc tgcatttttt gataataata aattagaggt tgttcttctg
    10621 cttctggcgc tttctcgata cctaacaaat aaagagcatc catttttcca gctgagcaga
    10681 tatctactaa aggtaaaagc tgatggatta cttcgctggc ttcttgctgt gtccataatt
    10741 ttccacgata gttgatatcg aagctgatca ggcagccagc tttttttgca gcttctatca
    10801 cctgtttcgt cattttttgc cattttttag acaaagctgg ggtgattcct gaaacatgaa
    10861 atatatctat tccttggaat aactcctcta gcgaccattc caaatgatcc atttcagcaa
    10921 aacttgatcc ggcacgatca tagatcactg aggcggcgcg ttcgccacta ccagtttcca
    10981 tataataagt acctagtctt gatccaccag ataatacctg tttcgtatct actccataac
    11041 attgcaagtg ctttttgact gcttttccca atgcgttatc aggtactttt gtagcaaaaa
    11101 tcgcttcatg tccgtaatta gctaaagaaa ttgctacgtt tgcttcccct ccgccataat
    11161 gggcagcgaa tcgttcactt gaatcgatac gggttccctg ctctgtggac aatcgcaaca
    11221 tgatttctcc gagtgtcact acttttccca taagcagcta cctcttgatt tctttcattt
    11281 ttgctgcata ctgttgcgcg acttccgtta ctttgtcaaa atctcctgta gctgcgggtg
    11341 ctaaaaggtt cccacctact ccgactgtta cgacaccagc atcgaaccac tctttcatat
    11401 tgtccagact cacaccgcca gttggcatga tattgacttg aggcatcggc gccttgaagg
    11461 cagaaatgat acttggtcca taagcgcttc caggaaatag tttgacgata tctaccccgc
    11521 ttttcagcgc tgttttgatc tcggtaatag tcatacatcc tggtaagtaa ggaatttgat
    11581 acagattgca catctctgcc gtttcttgat caaaggaagg gctaacaata tactctgcgc
    11641 cagctaagat agccaagcga gctgtgacag cgtctaatac tgtgcctgcg ccaatcacta
    11701 tttctggttg gtctttgtag aatgacaaaa gttctttgat tacttgatct gcttgaggga
    11761 ctgtaaaagt caactcaata ccagttagac cacctttgat gattgcgtgg caagcattca
    11821 aagcttcttc tttgcttttt ccgcgtacca ccgcaatcac tccggcgttt ttcaatcgag
    11881 ataaaatatc aatttttttc atcttctcca cctatttcct atttagaaat tttattttat
    11941 aacttggaaa tctataccca tattatacgg cagaaaagaa aaattgcaaa cgcttttttc
    12001 attatttttg aattctttgc tcgatttcgc tttttgtttg cttgagggca ctgattattt
    12061 gttcttttgt ctcatctgtt aaacgatatt tcggcatact tacactaaat gcaccaatga
    12121 tttgttcgtt tttcacgatt gaggctccta tacaaaaaat atcacgttcg atttcttcat
    12181 cgtcaaatgc gatgtgggtt tcttttaccg ttttgatttc ctttttcaac cgtaatgcat
    12241 tggtgatcgt atattctgtg taagggatca acggatgctt ttcaagataa tcagtgtact
    12301 gctcttcact gaattctgct aatacggctt tgcccatcgc agaattgtat aatggtcggg
    12361 tgattccgat ttttgaagac atgcggatcg tttgatgttt tggatctagt ttgttcacgt
    12421 acaagatctc attgttgtcc aataccccta agtggatagt ctcatcgatc ttttgctgca
    12481 gctgttctaa aaacggtaaa gtcaactcta ccaaatcgat ttgttcaatg tttttattcg
    12541 cgtagcgaat aagctttgca cctaaacgat agtttttctc tgaattttta tttacgtagc
    12601 cgatcatcaa caaagtatct agtattttca agactgtaga agaggtcatt cccacaccct
    12661 ttgcgatcgt ctgcaaagag acatcttggt tttccgctaa atagtctagt attttagagg
    12721 ctttgatcaa tacagttcca taaggctttt tattttccat ataatattct cctatttctt
    12781 tataagaaaa attatagcgg aaataccatc aatttaaaag agatatccag atataaacta
    12841 ccatttctct ttacaggcaa tttaatgttg aagatccgct tcgtttttac atcaaacttt
    12901 gatataaagc aatgatttct tctactgtcg tttctcttgg atttcctggt gtgcatacat
    12961 ctcgcaaagc atcttctgca attgctggaa tatctgcttc ttttacacct aattcagaaa
    13021 tggtttctgg gattccaacc gctttgctca accgatcgat ctcaccacaa gccgcttctc
    13081 ttacatcttc taaagacatt tcagctgctc ctttaatccc tagaacaaga gcgatctcac
    13141 ggtacttttc tccagtatat tctttgttat atttcatgac agttggcaaa agtgctgcac
    13201 acgctacgcc atgaggaatg ttgtaccatg cacttaacgg gtgagccatc ccgtgtacca
    13261 gtcctaaacc gacatttgag aagcccattc ccgcaatata ctgtcctaat gccatttttt
    13321 ctcgcccatt ttgatcgcca tctacagaag cttccaaaga atggccgatg atttcgattg
    13381 cttttaaatg cagcatatca gtcatttccc aagcgccttt tgtgataaac ccttcaatag
    13441 catgcgtcat agcatccata cctgttgatg cagccaattt tttcggcatt cccatcatca
    13501 tatcgctgtc gatgaacgcg acgattggga tatcatgagg atcaacacaa acaaattttc
    13561 ggtgatttac ttcatctgtg atgacgtagt tgatcgttac ttctgctgct gttccagaag
    13621 tcgttggtat agctaatata ggtaaacatg gatttttcgt atcagcaaca ccttctaaac
    13681 taatgacgtc tgaaaactct ggattcgtca aaataatccc aatggctttt gccgtatcga
    13741 ttggcgaacc tccaccgatt gcgatcaaac agtctgcctc tgcctcttta acaaaagcca
    13801 ctccatcttt tacattttgg atcgttggat taggtacgat cccgtcgtat aaagcataag
    13861 cgattgctgc tttatctaga agttctgtta cttttgttgc tataccgtgc tcgatcaatc
    13921 ctttgtcagt aatgacagct gctttgccga atcctcgctg ctgaaattca gggacgatcg
    13981 actcaatcgc tccttttcca aaatacgatg tttcatttaa aatcattcga ttactcattt
    14041 tctattcctc ctaatttgta ttatttttca caataaaaac gaaagaaccc caagcaaccg
    14101 gttgcccagt tgcttgttgc tacagttcaa agactggcat caattcccaa ctgactggag
    14161 aggcatctgg atttgtctcc atgatatctt ccatgtactt ccaccatttt tgattgatag
    14221 atgtatttgc cattttttcc cataactttt catcttcgat cactaagtaa ccaaataaca
    14281 aacttgtact cggatcaaga tagatcgaat agtcgatgac cccgtgttca cgaagtgctg
    14341 tacgcatttc tggccaaagt tcatcatgtc tttttttgta ttcttcttgt ttttctgggt
    14401 aaactttcat gcaaaaagct tttttcacca tgtcagacac ctgctttcgt atgaacttcc
    14461 tccaaataac ctgcttttgg agtgacacca aacgcatctg ctaaatgcca caggttttcg
    14521 tccgtgattg tttgcaagat tggtccttgc gctttgacgt acgtatagac ttctgctgcc
    14581 ttttctgctg tctcgatcaa accaaagact tcatccatgt cttttcctga cccgtagatt
    14641 ccatgttgtg gccataaaac caatcgagtt tctttcattt tttcagctgt tgcttcccca
    14701 atctcatttg ttcccggaac catccacgga atgatggaaa ttccttctgg aaaaacaact
    14761 aagcattccg tacacatttg ccatagcgta cgactaaagg aacgttcgtc taattcatgc
    14821 gtaaaactca tcgctaatag atgactagca tggcaatgca taacgatccg attatctggg
    14881 tctacttcta aacgggcgat atgactcatg aaatgacttg gcaactcgct tgtaggaagg
    14941 cattcgtttt ccagccccca caaaagttcc agtgttttgc catcttcagc aactcgaacg
    15001 attccaagat tctcggcagg ttcgctcgct acatttttaa aatatcttcc tgaaccagtt
    15061 acaataaaat agcatcctgc taaacggctg gcatcaaaaa tcatcgagat gcggcggatc
    15121 actttttcag gatccaagaa cggtgtgatc tcttcttctt ttagtaaata agaaatattc
    15181 ccgccatttc tttcatccca tcccaagcga taaagattcg acgtcgtttc gatcatttct
    15241 ttcacataag gcgcttgtaa aatatctatt tttttcaata tagccacact cctattttct
    15301 taattccaac acgtcttttt gatattcctg gacttcttcc agccaatcca aaccaactgg
    15361 aacttgattt ttgtaacaga aataattcca tacatcggca tacgggaaat ctttcaattc
    15421 ttccgtataa gccattcttg aagtaaagtc aaaagccagc tccatttctt tcaatcgctc
    15481 aactggttcc agcatagctt tcagcagtgc tttttgtgta tttctcgtac cgatgaccca
    15541 agcagcgata cggttgatcg ttgcatcaaa gaaatccaga ccgatatttg ttttttccaa
    15601 caattcgtta cggactaatt ctttagcgat ttcctgcaat tcatcatcca tcaaaaccac
    15661 atggtctgaa tcccaacgca ctggacggga aacatgcaac atgattcctt tgctgaataa
    15721 agctaatgaa gataatttat tggaaatcac ttctgttgga tgaaaatgtc cagcatctag
    15781 acaaacaagt ttgtcacgag tcaacccgta acccatataa aattcatggg aaccgactgt
    15841 ataagcttca gcgcctaaac caaatagttt gctttctact gcatcttgta cataccgttc
    15901 atctaactct tctgtaaaaa tttcatcgag tgattccatt aaacgttccc gtggggtaaa
    15961 acgatcaatc ggattatctt tcatcccatc aggtacccaa aagttattga tacttgtttg
    16021 cccagtttct ttcccaaagt actccgcgat tttgcggctt cttttcccgt gttcgatcca
    16081 gtattcacga acttctttat ctggatgagc caatgtaaag ccgtctttca tcattggatg
    16141 cgaaaagaaa gtcggattga agtcaagacc gatttcttct ttttttgccc actctaccca
    16201 tttttcaaaa tgtttcggct caatttcatt tagatcgaca gattcttctg tatccaaata
    16261 gatagcatga aggttcaatt tgtgtttgcc cggtatcaac gaataagcct tctccagatc
    16321 ttgtctaagc tcttcaggtg ttcgtgcggc tcctggaaaa ttccctgtcg ccatgattcc
    16381 accggtcaat tcgccatccg gtgtcaagaa acctttgaca tcatctcctt gccagcaatg
    16441 catagaaatt ttgatgtcct gtatcttttc tagtacagca tctgtatcta caccgatagt
    16501 tgcatatttt tcttttgctt gttcgtaatt tctttcaatc gtgttcatac gattactcct
    16561 ttgattttat tcacgtacca ttccatcgtg ttttgcttat tttcatctgg gtaaaaagtt
    16621 tcacatggaa acgactgttt gattacattt cttgcttgtt ctatattcga aaactgacct
    16681 gtactgagca tttgcatggc aacattcccg attgctgtgg cttcactagg tcctgcttct
    16741 acaatgattc cggcataatc tgctgtcaat tgattcagta gacgattatt cgatccgccc
    16801 cctacgatgt gcagcttttc caatttcgtt ccagttaagt gttctacttt gatcagctct
    16861 tgtgcgtaac aaagagcaag attatcatac acacaacgag cgatagctcc cgatgagaca
    16921 gggactggct gctctgtttc aaaacagtat tgctgaatct cttctatcat atttggggga
    16981 ttcaaaaatc gagggtcatt gatttccatc acttgctgca gcggtttttc ttttgcagca
    17041 agctctgcca attccgcata actgtactcg tactcttgca ttcgggcaac ttcctggatc
    17101 aaccacatcc ccatgatgtt tttcaaaaaa cgcaccgtgt tatgggcacc ccattcattc
    17161 gtataatttt cagaaaaagc attgcgagaa acattggcga tctttgtttc aatccctaaa
    17221 agcgaccatg tcccactgct gatataaccc caacgttctc cttgacctgg cgttcctaat
    17281 acagctgacg ccgtatcatg acttgctact gtgataaagg tcacatctgg caaatcatat
    17341 tttgaaaatt gttctttttg cagatctcct agtatcgttc ccggctctgt caatggtgga
    17401 aataattcct tcgagacacc gattttttca agaagttcta tttcccattc ttttgtccct
    17461 gcattcagca gctgcatagt agatgcatta gttttctccg tcaccatgtt accagtaaag
    17521 acatacccta aataatcagg tatcagtaac aatttatgtg cttttgccaa attttctttt
    17581 tcttcagcaa acaattggaa caaggtattg aaaggctgga tctgaatccc tgtctgctca
    17641 tacaacttat ctagagaata gatttcagaa aactgttcta ctgctccatt ggtacgcttg
    17701 tcccgataag caatcggatc atttatccga tccccttgtt catcaatcag acagtaatcg
    17761 actgcccatg tatcgatccc cgcaaaaagg cgatcaatcc ctgtctgctt taatttttca
    17821 aatccaatca aaatctcttt gataagataa tcgatcttcc agcgatcgtg accgtctttg
    17881 ttccgaaagc cgtttttgaa ccggtgtact tcttccaaga tcaaagaacc ttttgcatcc
    17941 ctcgtacttt tcattagtcg tccgcttgat gcaccgatat cgatggcaac aaatgttgga
    18001 ttcatttctc cacctctttt actttattgt tgattagcac tttattaata aagcgttatc
    18061 atttattgac aatgctatca taatacagaa tcttatattt tgcttcacac tattttggta
    18121 tataattgca ttatcttgac aagttgggag tgtttacatg gattactggg aaaaagccaa
    18181 ccactcatgg tcggaagact caaaaagagt gatcttgacg cctacacaaa gaagcaggaa
    18241 tttatttttt tatatccaag aaatcggtta cttcaaagca ccaatccctt actatactga
    18301 acgagcacat ttaccttctt acttgataaa gtttactttg tctggcaaag gttcgcttac
    18361 atataatgat aatcagcaac agctaaaagc tggagactta ttttttattg actgccaaga
    18421 atatcagcgt tatcaaacga tcagcgaaga accatgggaa atggattgga tccatttgaa
    18481 tggtcagaac gtccaatcat tttatcagga gttcacaaaa gacggccata acaccttcca
    18541 ttcaacagga gatccatcag aaaacaagat ccaccatttg atcacagaat tgatccacat
    18601 gcaacaaggc acgaatgccc gtaccgattt ccagtcctct gtagctatcc acgagttgct
    18661 gaatgaactg attttgcaaa agtatcagct agatttcgaa gaagaggata ttcccgccta
    18721 cgtttacaaa ctgaagcaat acttagacga acatttcaaa gaaacgatta cacttgatca
    18781 attagaacaa caattcttat tgaataaata tcagctgagc aaagattttt ccaaatttat
    18841 cggttctcca ccaatcgatt atttgatcag caaaaaactt tcttacgcta aagaccttct
    18901 tcggtataca gaatttacgg ttcgaatgat ttccgaagaa atagggattg agaattttgc
    18961 ttactttagc cgcctgttca aagcaaaaac aggactatcg ccgagagact ttcgtaaatt
    19021 cagctgattc atataatatg tacgtcattt tttcaaaata ttttgaaata tatagataaa
    19081 aataaaataa ccgcagtttc tgcttctatg taaacaacag cagctactgc ggttattttt
    19141 tagtgatcat tctcattgtt catcttcaaa atgttcataa tgaaccaact gtttctgtgt
    19201 atcacgtttc aaaaattctt taggcgttat gccgaactct ctcttgaatg ctttcagaaa
    19261 atgcggataa gaagaaaaac cacttttact atatacagca ctagagctgc ggtattctcg
    19321 caacaagtgc ttggcataga tcagcttttt cttcattaca tactgatgaa acgtaaatcc
    19381 agtgtacttt ttaaattctc gtgtgatatg gtatttgctg acaaaaaact gcttttccat
    19441 ttcctctaaa gaaagcggat gagataaatt ttttgagata tattgtaaca tttgttccac
    19501 acgctcatta ggaactacag cattctgttc ttgcgcactc tcttcttttt gtacaacttg
    19561 attgagaaaa atcaagtatt ctaccagagc ttgttcatat tttatgtctt caccgtaaat
    19621 ttttttatca ggaggatggt cgataaatga taatttttgc ttcaatactt cctgttctat
    19681 ttgcaataca cgactcctct tctctcccat tggttgaaaa caagccgata gattagacca
    19741 tggcgtggaa cgactttcta gaaaagaagg ggtgacaaag atatagacac gctcgaatac
    19801 atctgaagat tggcggacga tacgatgcaa atcattggaa tggatgagca gcacacttcc
    19861 tgctttgatc gtaaattgtc gtccgttcaa ataaaaagtc gcttctccct ttaatgtagc
    19921 atggatctca tagaaatcat gatgatgata gatcgtttta ttttggctga cgacatcttt
    19981 gacatgatag atttcatact gatcagaaac catcgtattg acttgttcac cgctgatttt
    20041 cttttggagc atgtgccgca cctccttttg ttcacttatg cacaatttta ttataccacg
    20101 ttttttgtaa tcaaaaagag ctgcttttat atactcttat aaaaaagaag agacgtggca
    20161 tcttctagcc ccgccccctc cattctaaat attagttaac caacgtcttc tttacattat
    20221 tcaagaagac ttcttccata acgaatccca tcatcttttg tttcttcaag aaccacatac
    20281 aggtacggac gattcttcat gatgatttct ttgatttttg tgtactcgat cacaccgtct
    20341 cccaaattta ccggcacgat tttttcctgt tcgacacgaa aatctttcaa atgaaccgca
    20401 catatctttt caccaaaacg ttcaaaagct tcttctacca attggacttg ttcttgataa
    20461 tcactacttg tgatcaaatt ggtcggatct aaaatgattc ctagatagtc cgaatctact
    20521 gcttggatca gctgctctac ccttgctaaa gaatacaccg gatgattcaa acctggttct
    20581 atccccacca tcatttggta tttttctcca gcttttacca agcgacggat aacggacaca
    20641 gcttcagcaa atgcttcatc tgtgaaattt tcttccgtat attggatttc cggcaaaaca
    20701 catccagttt ccgaagcaac catcgacgca ccaaaggaag aagcatagcg aagatactgc
    20761 tcgaactttg tcagcaactc ttgtcgtatc gttaaatctg gatgaatcat attgatatag
    20821 caacttaaaa ttcctacggt cacttcttgc ttttctaaaa ctcgtttgac ataatttccc
    20881 atcccagaat tgatctcatt tgcgccagaa ggtatttcag gaaacgaaag acctaaggcg
    20941 agctgcagtg tatgaatccc ttgttctttt gttttttctg caagatcttc tatcgtttgg
    21001 acttcggaaa gatcatgtcc tctaattgta atgttcaaca taactattct cctaactcag
    21061 catattgggt agccacatga cgatttgcgg gaagtaagta atcaaaaaca agacgactaa
    21121 gataacccca taaaaaggaa gaagcggttt cagcattgcc tctgctttta cgcctcctac
    21181 acttgcaccg acatatagtc ctgtacctac tggcggcgtg atggagccta cacacagatt
    21241 catgattgaa aacaacccgt aatgtacagg atcaacccct acacttgtga cgattggtaa
    21301 aaatattggt gtaaaaatca agatagcagg cgccacatcc ataaacatac ccaccagtaa
    21361 taacacaaga ttgactaata acagaataat aaatttatta tccgttacgc ccataatcaa
    21421 cgaactaatt gcctgaggaa taccggtaaa cgccatggcg aaagacatca tggaagaagt
    21481 agccaataag aacatgatcg tccctgacat tgctactgat tgaaacaaca tattaggtat
    21541 atcttttagt tttaccgttt tatagtaaaa catagcaatc aacagggagt aaatgacagc
    21601 tatcgctgaa gcttctactg ctgtaaatat ccccgtcaag ataccaccga taatgatgac
    21661 aatcaataga aggctaggaa tcgcctcttt tattattttt ccagcatgag ccatctgtcc
    21721 tttttcgaca acagggtatt tattcttttt agcaacgatg aatgctacta gcatgattcc
    21781 tagcgcccac aaagcgccta cgagataacc tcccataaac aaagcagaga tcgaagcacc
    21841 accagcaaca agtgaataca tgataaaagc agtacttgga ggaatgacca tacctgtcgg
    21901 agctgacgcg atattgaccg ctgtcgcaaa cttccggtca tatccttctt ccacttgctg
    21961 agggatcaac actccgccaa tcgcggtaga agcggcaatg gccgagctag agatggaacc
    22021 aaataaagca ttccctaaca cattcgtatg agccaaagcc cccggcacgc gcccgacgaa
    22081 caacatcgca aaatccacta gtttcttagc tatccctcca ttattcatga taatgcccga
    22141 gagaacgaaa aacggaatag cgatcagcga aaaactattg atacttccca ccattttttg
    22201 agctgaacta aaagcagaaa catcaaacgg tataactaac aataaagtca tcatcgatga
    22261 gaatgcaata cttatagcaa tcggcatccc taatacaagc aacacaacaa atacaaaaaa
    22321 taacactaat ccggcttcta ttgccatttt attcctccat ttttatattt tttcttgaat
    22381 tgctggcttg ttgcttaatg tatcgattag attgatcagg ctatagataa tgatgagtac
    22441 ccccgataca ggtactgaca gataaacata tcccatagga atacttaaag aaggcgagat
    22501 ctgccccatt gtcaatgtga cagcattcca tccgccatac accatgatca tcgcagcaaa
    22561 taccagaaat aaactttgaa caacaacttc taaaatttta ctttttttgc cagttaattt
    22621 ttcacttaaa accgttacgg ctaaatgtgc tttttgtccc acaacatacg cggaagacaa
    22681 catggcaaac cagacaagac cgaatcgaac aagctcttcc gtgaccgtat ttggatttcc
    22741 taaaaccgtt ctggaaaata cttgccataa aacaacagcg accatcccaa caagcaaaac
    22801 agctcccagg atctctaata ccttgtttaa aatcattttc atcacagcta cgctccttgc
    22861 tcttggatct tttgataaag gtctttgtag gtaggatcgt ttttgaatat atcatgaagt
    22921 ggggcaactg cctctcgaaa cggtgtatta tctacatcat tgaattggac tccatgttct
    22981 tcttgtgcta aagctttttc ttcttcaatt gcttttgcaa aagcttcaat ttcaaaaacc
    23041 gttgattctt tcgctgcttc ttcaatggct ttttcctgct catcagttag ctgttctttg
    23101 atggcatcat tcatgatgat gatatctggc acacgtgtat gctcatcata ggaatagtat
    23161 ttagccacac caccatgtcc agctgtatac aagacaaatt cattgttttc tgatccgttg
    23221 atcaaattgg attgaagaga cgtgtacact tcatcacttc ccataggtac gggcgaaccg
    23281 cctaaaagct ctaccatttt gatagcggtt tcgctttgca tcacacggat cttctttccc
    23341 ttcaaatctt ccggcgtatg gatcggaccg tctaccatat aaaagctgcg ctgaccagaa
    23401 tcataatagg tcaatccggt aaatcctaaa tttttggtcg attggtagat tggctccatt
    23461 atttcttcat tatccatcac cttaaagaaa tgttcttcat cattgaatag ataaggaact
    23521 gaaaaaatcg aataatcttt agagaaactt tccaacgctg aggcacttac ttttgtgaaa
    23581 tctactgctc ctgactgtgt caattcgatc agttcacgtt ctccacccag ttgtccatct
    23641 gggaaatact ggatctgtac ggaaccatct gttttttctt ttactaaacg cccaaattca
    23701 ttcattgcat caattactgg ctggctgttg cttgcataag caaagcgcaa ggtaactatc
    23761 tcatttacat ctgcatctgc gacagttgcc acactagaat tatttccgca gccgcttaga
    23821 cctagcagac ctaggctaag cgaacttgct gcaaacagca gtgatgtttt tttcatatgg
    23881 gtttcctttc ttttaacaat aagagacgag caattctgtc aaacaaagca ccgttaacga
    23941 ctgtccataa ggcattgctg tgattccgat attccgataa aaatctaaac tgtcccccat
    24001 tcctgtcccg attgaaacgt tttgaacttc tcctttttca tcgatttgtt caagcaatcc
    24061 ttggatagca cgataagcga cttctttata ttcttgtggt aaataacgtt tatggaccgc
    24121 tttcaaaata ccgtaagcaa atcctgcagt tgcagaagat tctacgtaag aagaaggatc
    24181 atccaacaac gtatgccata aaccgctttc agactgataa gtttttaatg cacgcacttg
    24241 tgcttcaagt gtttcgatca ataatttgcg caatgcatct tctttcgata attctaagat
    24301 ttcaatgatc tctggaatag caatcgtcaa ccagcagttt ccacgagccc aaaatgcttc
    24361 ggcatagtta tgatttcctt caaacgtcca gccgtgatac cacaatcctg ttcgtttatc
    24421 catcaagtat ttgatatgga tcataaactg gtaacgagct tcttctaaat attctgggcg
    24481 attcaacagt ttcccgattt ttgccaaagg aagcactgtc atcatcaatg tatcgtccca
    24541 caactgattc ttattttccg gtccatatgt ggcatgctgt aacccgtctt catttgtccg
    24601 aggcatgtca ttcatgaccc attctgccca ttgttctaaa taaggcaaat agcgtgaatc
    24661 tttcgtgtct tcatacaagt aagccatagt caatagcggt gccattgtat tgacgttttt
    24721 gggcggagcg ccttcttcca tacgatcctc aaaccattca ttgaccactt gataagcgcg
    24781 aggatttttt gtcatctggt aatttttata aataccatat agaccgacac cttgtggcca
    24841 attccacact gtccagcttt tgtcatctac tttcagtcca tcaaagtcca acaaaaattc
    24901 accattatcg tctttgatcg ttgtcaggtt ttcaacgagc aggtcaattt tttctttcac
    24961 taattcttta ggtacaaaaa tctcttcttt aataatcatc tttacgccac ctttttaaac
    25021 gctttcattt tatatcctta atatagcagt aataccgtcc actataaata tcgctttttg
    25081 aacaaaaact gtctaaaatt gacctaaatg ttaaataaca aaagtaactt tcagttactt
    25141 ggaaattaaa aaaagaactt ggagaaacaa cgttttccaa gttctttttc atctattttc
    25201 ttatcttttt tctacgcttt cttttgctta tccgttgtaa gcatcaatga tgatctgttt
    25261 caattcagag atcaatggtt ctttcggatt agctgtcgta cattggtctt cgtaagctaa
    25321 ttcagccata cggtctacgg ttgtatctag tgtttcttgt gtgactcctt gagcttttag
    25381 actcatgttg atgccgacag attttcctaa ttcataaaca gccgttgcta aagcttcgac
    25441 taattcctct gttgtttcgc ctttcagacc taagaatttt gcgatatctg cgtaatctgt
    25501 atctgcacgg aaatagtcat atttagggaa catcgcatgt ttttgtggat cttttgcatt
    25561 atagcggata atatgcggta aaagaatcgc atttgtccgt ccatgaggga tcccgtattc
    25621 cccgccgatt ttatgtgcga ctgagtgaca aatacctagg aatgcgttag caaacgccat
    25681 accagccatt gtagaagcgt tgtgcatttt ttcacgtgat tccatatcag gtgtttttac
    25741 tgatttttct aaatagtcaa agactaactt gattgcttgt aggctcaatc cacgtgtata
    25801 atctgaagcc atgactgaaa cataggattc gattgcatgt gttaacacat ccatcccggt
    25861 atctgctgtg acagatgctg gaactgacat gacgaattga ggatcgacga ttgcgacatc
    25921 tggcgtcaat gcataatctg ctaacggata tttcacatgt gtttcactgt cagtgatgac
    25981 cgcaaatggc gttacttcag aaccagtccc tgatgttgtc gggatacata cgaattgtgt
    26041 tttttctggt ttgtcgatct tatatgtccg tttacggata tccaagaatt tttgttttgc
    26101 accaaagaaa gatgtgtctg ggtgttcata gaacatccac attcccttag ctgcgtccat
    26161 tgcagaaccg cctccaagag cgattactgt atctggcttg aagtctgcca tcagtttcgt
    26221 tcctgcgtat acggtatttg tcgatgggtt cggttcaaca tctgagaaga cttcgatctt
    26281 cacatcattt ttacgtcgtg ccaatacttc acgaactgta tcgcagtaac cgaattggac
    26341 cataccaggg tcacaaacga tcatgacacg ttcaacgttt tccattttct ctaagtacaa
    26401 tagagagttc ttttcaaaga agatttttgg cggtaatttg aaccattgca tgttatttct
    26461 ccgtttcgct acagttttga cattgattaa gtttacagca gagacgttac gagaaacaga
    26521 gttcttacca taagaaccgc atcccaaagt caatgacgga atcatttcgt tatagatatc
    26581 accgatgccg ccttctgcag atggtgtatt cactaaaata cgacaagctt tcattcgaag
    26641 accgaatttg acatgcaact cttcgttttc tgaatggata actgctgtat gtcctaatcc
    26701 tccaagattc aacatacctt cacacaaatc aaacgcatgg tccgtgttgt tagctttgat
    26761 catggcaaga actggagaaa gtttttctct tgatagcgga taatctgggc ctacgccttc
    26821 caattcagca atcagcattt tcgttccttt tggtacactg attcctgcca attttgcgat
    26881 ttccatcgca gaatgtccaa cgattgatgg attgaccgca tattttcctt cgttcatcac
    26941 tgcatcttct aatttttgca attcatctgg ttttacgata taaacttggt gtgcttggaa
    27001 ttctgctttt actgctgcat agatttcttt atccacgatc acagcttgtt cagaagcaca
    27061 aatcattcca ttatcaaatg ttttcgaaac gatcaaatcg ttcactgcac gtttaatttt
    27121 tgctgttttt tcaatataag ctggtacgtt acctggacct acacctaaag ctggttttcc
    27181 tgtagagtaa gcagacttga ccattccagc tccacctgtc gctaagacga tcgcgatacc
    27241 tggatggttc attagcatag aagtcgcttc gatcgaagga tgttcgatcc attggataca
    27301 attttcagga gctccggcag cgatagcagc atcacgtacc actcgtgctg cttctgcaga
    27361 agatttttgg gcacttggat ggaaagcaaa aacgattgga tttcttgttt tcaacgcgat
    27421 cattgatttg aaaatcgttg tggaagtcgg attcgttgtt ggagttactc cgcaaaccac
    27481 accgactggt tcagcaattt caatcaaccc tttttggaca tcttcattga tcacaccaac
    27541 tgttttatca tgtttgatat tatgccaaat atattcggac gcatacatat ttttgattgc
    27601 tttatcttcg taaacaccgc ggcctgtttc ctctacagcc atcttcgcta atggcatatg
    27661 ttgatccaaa gctgccattg ccatttcatg cacgatatgg tctacttttt cttgatcaaa
    27721 gctttccatt tctttcaatg ctgcatttgc tttttctgcc aaatcatcaa tcattgcttg
    27781 tacgtcaatt gtcttcgttt tttctttttc tattacttta gccatcatta tcctcctaat
    27841 gggtcatggt tatttgttaa ataattcaca tgtatatcat acaatattta tttttctatg
    27901 taaacacttt tttgtgattt ttttcacagt tatttttaaa tataaaattg ctttatttac
    27961 aggt
//
LOCUS       DAYYCY010000017        27581 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000017 DAYYCY010000000
VERSION     DAYYCY010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27581
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.017"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..580)
                     /locus_tag="ACHAZB_000609"
     CDS             complement(<1..580)
                     /locus_tag="ACHAZB_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8613309.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(713..1396)
                     /gene="rpiA"
                     /locus_tag="ACHAZB_000610"
     CDS             complement(713..1396)
                     /gene="rpiA"
                     /locus_tag="ACHAZB_000610"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFM8613310.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            1715..2128
                     /gene="rpsL"
                     /locus_tag="ACHAZB_000611"
     CDS             1715..2128
                     /gene="rpsL"
                     /locus_tag="ACHAZB_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFM8613311.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            2212..2682
                     /gene="rpsG"
                     /locus_tag="ACHAZB_000612"
     CDS             2212..2682
                     /gene="rpsG"
                     /locus_tag="ACHAZB_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFM8613312.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            2776..4863
                     /gene="fusA"
                     /locus_tag="ACHAZB_000613"
     CDS             2776..4863
                     /gene="fusA"
                     /locus_tag="ACHAZB_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFM8613313.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            5035..6222
                     /gene="tuf"
                     /locus_tag="ACHAZB_000614"
     CDS             5035..6222
                     /gene="tuf"
                     /locus_tag="ACHAZB_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFM8613314.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            6629..6937
                     /gene="rpsJ"
                     /locus_tag="ACHAZB_000615"
     CDS             6629..6937
                     /gene="rpsJ"
                     /locus_tag="ACHAZB_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFM8613315.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            6968..7597
                     /gene="rplC"
                     /locus_tag="ACHAZB_000616"
     CDS             6968..7597
                     /gene="rplC"
                     /locus_tag="ACHAZB_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFM8613316.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            7625..8248
                     /gene="rplD"
                     /locus_tag="ACHAZB_000617"
     CDS             7625..8248
                     /gene="rplD"
                     /locus_tag="ACHAZB_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFM8613317.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            8248..8538
                     /gene="rplW"
                     /locus_tag="ACHAZB_000618"
     CDS             8248..8538
                     /gene="rplW"
                     /locus_tag="ACHAZB_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFM8613318.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            8577..9410
                     /gene="rplB"
                     /locus_tag="ACHAZB_000619"
     CDS             8577..9410
                     /gene="rplB"
                     /locus_tag="ACHAZB_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFM8613319.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            9453..9731
                     /gene="rpsS"
                     /locus_tag="ACHAZB_000620"
     CDS             9453..9731
                     /gene="rpsS"
                     /locus_tag="ACHAZB_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFM8613320.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            9754..10101
                     /gene="rplV"
                     /locus_tag="ACHAZB_000621"
     CDS             9754..10101
                     /gene="rplV"
                     /locus_tag="ACHAZB_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFM8613321.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            10115..10771
                     /gene="rpsC"
                     /locus_tag="ACHAZB_000622"
     CDS             10115..10771
                     /gene="rpsC"
                     /locus_tag="ACHAZB_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFM8613322.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            10774..11208
                     /gene="rplP"
                     /locus_tag="ACHAZB_000623"
     CDS             10774..11208
                     /gene="rplP"
                     /locus_tag="ACHAZB_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011375443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFM8613323.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            11198..11386
                     /gene="rpmC"
                     /locus_tag="ACHAZB_000624"
     CDS             11198..11386
                     /gene="rpmC"
                     /locus_tag="ACHAZB_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFM8613324.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            11411..11677
                     /gene="rpsQ"
                     /locus_tag="ACHAZB_000625"
     CDS             11411..11677
                     /gene="rpsQ"
                     /locus_tag="ACHAZB_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFM8613325.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            11735..12103
                     /gene="rplN"
                     /locus_tag="ACHAZB_000626"
     CDS             11735..12103
                     /gene="rplN"
                     /locus_tag="ACHAZB_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFM8613326.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            12140..12448
                     /gene="rplX"
                     /locus_tag="ACHAZB_000627"
     CDS             12140..12448
                     /gene="rplX"
                     /locus_tag="ACHAZB_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFM8613327.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            12476..13015
                     /gene="rplE"
                     /locus_tag="ACHAZB_000628"
     CDS             12476..13015
                     /gene="rplE"
                     /locus_tag="ACHAZB_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFM8613328.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            13034..13219
                     /locus_tag="ACHAZB_000629"
     CDS             13034..13219
                     /locus_tag="ACHAZB_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HFM8613329.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            13255..13653
                     /gene="rpsH"
                     /locus_tag="ACHAZB_000630"
     CDS             13255..13653
                     /gene="rpsH"
                     /locus_tag="ACHAZB_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFM8613330.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            13685..14221
                     /gene="rplF"
                     /locus_tag="ACHAZB_000631"
     CDS             13685..14221
                     /gene="rplF"
                     /locus_tag="ACHAZB_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFM8613331.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            14382..14738
                     /gene="rplR"
                     /locus_tag="ACHAZB_000632"
     CDS             14382..14738
                     /gene="rplR"
                     /locus_tag="ACHAZB_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFM8613332.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            14759..15259
                     /gene="rpsE"
                     /locus_tag="ACHAZB_000633"
     CDS             14759..15259
                     /gene="rpsE"
                     /locus_tag="ACHAZB_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFM8613333.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            15274..15453
                     /gene="rpmD"
                     /locus_tag="ACHAZB_000634"
     CDS             15274..15453
                     /gene="rpmD"
                     /locus_tag="ACHAZB_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFM8613334.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            15497..15937
                     /gene="rplO"
                     /locus_tag="ACHAZB_000635"
     CDS             15497..15937
                     /gene="rplO"
                     /locus_tag="ACHAZB_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFM8613335.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            15937..17232
                     /gene="secY"
                     /locus_tag="ACHAZB_000636"
     CDS             15937..17232
                     /gene="secY"
                     /locus_tag="ACHAZB_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFM8613336.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            17294..17941
                     /locus_tag="ACHAZB_000637"
     CDS             17294..17941
                     /locus_tag="ACHAZB_000637"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFM8613337.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            18132..18350
                     /gene="infA"
                     /locus_tag="ACHAZB_000638"
     CDS             18132..18350
                     /gene="infA"
                     /locus_tag="ACHAZB_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFM8613338.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            18383..18499
                     /gene="rpmJ"
                     /locus_tag="ACHAZB_000639"
     CDS             18383..18499
                     /gene="rpmJ"
                     /locus_tag="ACHAZB_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFM8613339.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            18517..18882
                     /gene="rpsM"
                     /locus_tag="ACHAZB_000640"
     CDS             18517..18882
                     /gene="rpsM"
                     /locus_tag="ACHAZB_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFM8613340.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            18910..19299
                     /gene="rpsK"
                     /locus_tag="ACHAZB_000641"
     CDS             18910..19299
                     /gene="rpsK"
                     /locus_tag="ACHAZB_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFM8613341.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            19379..20317
                     /locus_tag="ACHAZB_000642"
     CDS             19379..20317
                     /locus_tag="ACHAZB_000642"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFM8613342.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            20345..20725
                     /gene="rplQ"
                     /locus_tag="ACHAZB_000643"
     CDS             20345..20725
                     /gene="rplQ"
                     /locus_tag="ACHAZB_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFM8613343.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            20997..21836
                     /locus_tag="ACHAZB_000644"
     CDS             20997..21836
                     /locus_tag="ACHAZB_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM8613344.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMIFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            21812..22681
                     /locus_tag="ACHAZB_000645"
     CDS             21812..22681
                     /locus_tag="ACHAZB_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM8613345.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            22671..23468
                     /locus_tag="ACHAZB_000646"
     CDS             22671..23468
                     /locus_tag="ACHAZB_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFM8613346.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            23671..24417
                     /gene="truA"
                     /locus_tag="ACHAZB_000647"
     CDS             23671..24417
                     /gene="truA"
                     /locus_tag="ACHAZB_000647"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFM8613347.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(24750..27419)
                     /locus_tag="ACHAZB_000648"
     CDS             complement(24750..27419)
                     /locus_tag="ACHAZB_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HFM8613348.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDGGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
BASE COUNT         9007 a         5129 c         5873 g         7572 t
ORIGIN      
        1 agatgcataa ttgctcgatt tctttttcag gtaaattgat atatttataa catgttccaa
       61 ctgaaacacc gcattttgaa gaaataaatt ggatcgtttc ttttttttca taatagagac
      121 gtcggatttt tttgatcgtt tttgcatcga tttttggtct tccgccgatt tttccttttt
      181 tacgagcttc atctaatcca actaatgtac gttctttgat caatgcgcac tccatgtttg
      241 ctaggttttc cattaattgg aaatagattt ttcccatttc gcgcgtatcg attccttcat
      301 caagactaat caagtgcagc ccgcgatctt tgaataattg cgtgagttct gttaattgcc
      361 gcgtagattt gcctaatcga tgaagttggc aaatgactag tgcatcgcct tgttgcatgt
      421 cagcgatcac tgactccaaa tgaacagcag tagaatcatt ttgttccact tcatatgtct
      481 catggaaaat ttcttgacaa ccaaagtcag ataattgatc aacctgagtt gttaaatcaa
      541 cgtcatttat ggttgtgcgt gcataaccaa taattttcat ggtgattcct ttctagcagt
      601 gattttctaa ctggatataa cagtaaaaat gtagcataaa gtacgtcaaa aagatagttt
      661 tttcgcaaat tttccgctgt ctttttttat tggatgatca agcagttttt ctttatttac
      721 gacggattgc gtcaagaatc tcaacgcctt tttctgagcc gacaactact ttagaaacca
      781 tgtttaggaa aagcccatgt tctactaccc caacaagttc atctaaatag gtagctaagc
      841 tttctggctg ttcgatgact tctagatgca gatcaatgat gtaatgacca ccatctgtgg
      901 ttaagacttc tccgttttcg tctgtacgca agacgggttg gaatcttttt tcttcaaaaa
      961 tatgaagtag ctgttgggag ccataaggga tcacttcaac tgggagtggg aacttcccaa
     1021 gtttatcgac taatttagaa gaatcaacga tccaaatcgt ttcttttgag tacgtagcga
     1081 ctattttctc gaaaagcaaa gcagcgccgc cgcctttgat cccttgaaaa tcagcactga
     1141 tttcatcagc accgtctatt gtcaaatcga ctacggggac ttcatcaata ctctttaacg
     1201 gtattcctaa tttttccgct tgttcttttg tcgcatttga agtagtaact cctgtgatac
     1261 gtaaaccttc ttcacgcata cggcgaccaa tctcttctac catataatag gcggttgaac
     1321 cggtcccgag acctacgatc atcccgtcct ttacgaattt cgcggcttca atgccagcta
     1381 attgttttaa attcatcttt gcgtccacct ttttttagtt tgagcatcgt gctttcctca
     1441 ctattttagc gtgatttacg gtaaaaagat accattcttt tttattccaa taaagctttt
     1501 tcaaaaaaaa ttgcatctct tgttgacatg ttttttcatt cgtgatattc taacaaaggt
     1561 gctttatcta caggtttctg tgaagaaacg tgctgactgt ttatcaaagc atatttgata
     1621 aggatgcaga cattgcattt attttttatc acaaaaaaag aaccacctgg atgtgtggaa
     1681 ctagacgcaa atcgtgggaa ggaggaaaca aggaatgcct acaattaacc aattagtacg
     1741 taaacctcgt aaatcaaagg tggaaaaatc taattcacct gctttgaaca aaggatataa
     1801 cagctttaag aaaactcaaa ctaacgtaaa ctcacctcaa aaacgtgggg tagcaactcg
     1861 tgtgggtaca atgacaccta aaaaaccgaa ctcagcttta cgtaaatatg cacgtgttcg
     1921 tttgtcaaac ttaattgaag ttactgctta tatcccaggg attggtcata acttacaaga
     1981 acacagtgtt gtattattac gcggtggtcg tgtaaaagac ttaccagggg tacgttacca
     2041 tatcgttcgc ggtgctttag atactgctgg tgttaacgac cgtaaacaaa gccgttctaa
     2101 atacggaact aaaaaaccta aagcttaatt ttaattatta aacaaatgaa acttgcttct
     2161 actatataga agtaattact atgaacaata tttcggaagg aggagttacg gatgccacgt
     2221 aaaggtcctg ttgcaaaacg tgatgtttta ccagatccta tttataactc aaaattagta
     2281 actcgcttga tcaaccgtgt aatggttgac ggaaaacgcg ggattgcagc taacattatc
     2341 tataatgcct ttgacttgat caaagaatct acaggtaacg atccattaga agtgtttgaa
     2401 caagcaatga aaaacgttca acctgttctt gaagttaaag cacgtcgtgt tgggggttca
     2461 aactaccaag taccagttga agttcgccct gaacgccgcg ttactctagg tcttcgttgg
     2521 gtagtaaact acgctcgctt gcgcggtgaa cacacaatgg aagaacgctt agcaaaagaa
     2581 atcatggatg ctgcaaacaa cacaggtgct tctgttaaaa aacgtgaaga cactcataaa
     2641 atggctgatg ccaaccgtgc gtttgctcac tatcgctggt aagattctct ctgtttggtc
     2701 ggcttttata agtcgacctc agatagtgtc cttattttta gcaactagat agtaaagaga
     2761 ggagaaacaa ggagaatgac cagagaattt tctttacaaa acactcgtaa tatcggtatc
     2821 atggcccaca tcgatgctgg taaaacgact gctactgaac gtatcttgta ctatactggt
     2881 aaaatccata aaattggtga aactcatgaa ggagcttcac aaatggactg gatggaacaa
     2941 gaacaagaac gtggtattac gatcacttct gctgctacta cagcacaatg gaagggtcac
     3001 cgaatcaaca tcatcgacac accaggacac gtagacttca cagttgaagt tgaacgttca
     3061 ttgcgtgtac ttgatggtgc tgtaactgtt cttgatgcac aatcaggtgt tgaacctcaa
     3121 actgaaacag tttggcgtca agcaacaact tatggtgtac ctcgtatcgt atttgcaaac
     3181 aaaatggaca agatcggtgc tgacttctta tattcagtat caactttaca tgaccgtttg
     3241 caagcaaacg ctcacccaat ccaattacca attggtgcgg aagacgactt cacaggaatt
     3301 atcgaccttg taacaatgaa ggctgaaatg tatacaaacg atcttggaac agagatcgaa
     3361 gaaacagaaa ttcctgaaga ataccgcgaa ttagctgaag aatggcgcga aaaattggtt
     3421 gaagctgtag ctgaaactga tgaagaactt actttgaaat acttagaagg tgaagaaatc
     3481 actgaagctg agttaaaaga aggtatccgc cgcgcaacag ttaacgtaga attctaccca
     3541 gtactatgtg gttcagcatt taagaacaaa ggggttcaat tactattgga cgctgttctt
     3601 gattacttgc catcaccgtt agatatccca gctatcaaag ggattgaccc taaaactgat
     3661 gaagaagtag aacgtcctgc agacgacagc gctcctttct cagctttagc atttaaagtt
     3721 atgactgacc cattcgtagg tcgtttaact ttcttccgtg tgtactctgg tgttttacaa
     3781 tcaggttcat acgtacaaaa cgcaacaaaa ggcaaacgtg aacgtgttgg tcgtatccta
     3841 cagatgcatg ccaactctcg ttcagaaatc tctgaagttt acgctggaga tatcgctgct
     3901 gctgttggtt tgaaagacac aactactggg gacacactat gtgatgaaaa gagtctagtt
     3961 attcttgaat ccatggaatt cccagaacca gttatccaag tcgctattga acctaaatca
     4021 aaagctgacc aagataaaat gggtgtggct ttacaaaaac tatctgaaga agatcctact
     4081 ttccgtgctg aaacaaacgt ggaaacaggc gaaacaatca tcgctggtat gggtgaactt
     4141 cacttagata tcatcgttga ccgtatgcgt cgtgaattca aagttgaagc taacgtaggt
     4201 gcgcctcaag tatcttaccg tgaaactttc cgtgctggca ctcaagccga aggtaagttt
     4261 gtacgtcagt ctggtggtaa aggtcaatac ggtcacgtat ggattgaatt tacaccaaat
     4321 gaagaaggtg ctggtttcga atttgaaaat gcgatcgtcg gtggggttgt tcctcgtgaa
     4381 tacattccag ctgttgaaac tggtttgaaa gatgctatgg aaaatggtgt cttagctggc
     4441 tatccattag tagatatcaa agctaaactt tacgatggtt cataccatga tgtcgattca
     4501 aatgaaactg ccttccgtgt tgctgcttct atggcattac gtgcagcagc taagaaagct
     4561 aatccagtaa tccttgaacc aatcatggct gtagaagttg ttatccctga agattattta
     4621 ggtgatgtta tgggtcacgt aactgctcgt cgtggacgtg ttgaaggtat ggaagctcgc
     4681 gctggcggcc aacaagttgt tcgtgcgatg gttccacttg ctgaaatgtt tggttatgct
     4741 acaactcttc gttctgctac tcaaggtcgt ggtacattta caatgacatt tgaccactac
     4801 gaagatgtac caaaatcagt acaagaagaa atcattaaga aaaacggcgg aaaagctgaa
     4861 taatttctta aaaccggata gtcctgcaaa gagattatcc aaagagacta tatttttaat
     4921 atagtttcgt gtaaaataat tatcgatgat atgggcatgt ggtgtacctt gtgtacatca
     4981 tatacctatc agacaaataa aaaatatatt gtatttttag gaggacattt taaaatggca
     5041 aaagaaaaat ttgaccgttc taaaccacac gttaacattg gtactatcgg acacgttgac
     5101 catggtaaaa ctacattaac agctgcaatc acaactgtac tatctaagaa aaacggcggc
     5161 caagctatgg cttacgatca aatcgatggt gctccagaag aacgcgaacg cggaatcaca
     5221 atctcaacag ctcacgttga gtatgaaaca gatacacgcc actacgctca cgttgactgc
     5281 ccaggacacg cggattacgt taaaaacatg atcactggtg ctgctcaaat ggacggagct
     5341 atcttggtag tttctgctgc tgacggccca atgcctcaaa ctcgtgaaca catcctattg
     5401 tctcgtcaag ttggtgttcc ttacatcgtt gtattcttga acaaagtaga catggttgat
     5461 gacgaagaat tactagaatt agttgaaatg gaagttcgtg acctattaac agaatacgaa
     5521 ttccctggtg acgatgttcc tgtagttgct ggatcagctt tgaaagctct agaaggcgac
     5581 gcttcatacg aagaaaaaat tcttgaatta atggctgcag ttgacgaata catcccaact
     5641 ccagaacgtg acaacgacaa accattcatg atgccagttg aagacgtgtt ctcaattact
     5701 ggacgtggta ctgttgctac aggtcgtgtt gaacgtggac aagttcgcgt tggtgacgaa
     5761 gttgaagttg ttggtattgc tgaagaaact tcaaaaacaa cagttactgg tgttgaaatg
     5821 ttccgtaaat tgttagacta cgctgaagct ggagacaaca ttggtgcttt actacgtggt
     5881 gttgcacgtg aagacatcca acgtggacaa gttttagcta aaccaggtac aatcacacct
     5941 catacaaaat tctctgcaga agtatacgtg ttgacaaaag aagaaggtgg acgtcatact
     6001 ccattcttca ctaactaccg tccacaattc tacttccgta caactgacgt aacaggtgtt
     6061 gttgaattac cagaaggaac tgaaatggtt atgcctggcg acaacgtaac aatggaagtt
     6121 gaattaatcc acccaatcgc tatcgaaaac ggtactaaat tctcaatccg tgaaggcgga
     6181 cgtacagttg gtgccggtgt cgttacagaa atcaaagctt aattcactta agttttaaag
     6241 taaagcaaca tttattcgga cgtaatcgct cggaacgaag gtcttaggat ctttgttccg
     6301 agctttttta tttattgcag gatcaggaaa aaagacagga gtattataaa tagaagaaac
     6361 acgaaataaa agagtcagta ttttctaaat gaaactttat gaaaacaaaa tgttttttac
     6421 aagaaaaagc cttgctttgt gagtttatat atattataat gacaaaggtg cgtttacttt
     6481 agtgaattgc acagacgtaa tcagaatgat ttcgtcggct aagaaacgag aggttgcgac
     6541 acgcccggac gctttgccac gaacgagcgt gacggaaatt ttcgtggagc tatgtctact
     6601 tttcaaaata ggcgaaggag ggaacaagat ggcaaaacaa aaaattcgta tccgtttaaa
     6661 agcgtatgaa caccgtattt tagatcaatc agcggataaa atcgtggaaa ctgcaaaaag
     6721 aactggagct agcgtgtctg gtccaattcc attaccaact gaacgcagtc tttacacaat
     6781 catccgtgcg actcataaat acaaagattc acgcgaacaa ttcgaaatgc gtacacacaa
     6841 acgtcttatc gacattgtga acccaacacc aaaaactgtt gatgctttga tgaagctaga
     6901 cttaccatct ggtgtaaaca tcgaaatcaa attataaaac taaaacaaaa ataatggagg
     6961 tgtactcatg accaaaggaa tcttagggaa aaaagtggga atgacacaaa tcttcactga
     7021 atctggtgaa ttaattccag taactgttgt agaagctact ccaaacgtag tactacaagt
     7081 gaaaacaatg gaaacggatg gctacgaagc tattcaagtt ggttaccaag atatgcgtga
     7141 agttttatca aacaaacctg cgaaaggtca tgttgcaaaa gcaaacacgg ctcctaagcg
     7201 cttcattcgt gaattcaaaa atgttgagct aggagaatat gaagtaggaa aagaaattac
     7261 agtagatgtt ttccaagcag gagacattat tgatgtaaca ggtactacga aaggtaaagg
     7321 attccaaggg gttatcaaac gccacggaca aagccgcgga ccaatggctc acggttctcg
     7381 ttaccatcgt cgtcctgggt caatgggtcc agttgcacct aaccgtgtat ttaaaaacaa
     7441 acgtcttgct ggccgtatgg gtggcaaccg cgtaactatc caaaacttgg aagttgtacg
     7501 tgtcgatgca gaaagaaatg ttatcttgat caaaggtaat gttcctggag ctaaaaaatc
     7561 tttaattact atcaaatcag ctgtgaaagc taaataattg taggaagaaa ggaggaacta
     7621 aggaatgccg aatgtagcat tattcaaaca agatggaagc caaaacggtg aaatcacact
     7681 taacgaagaa atcttcggaa ttgaacctaa tgaaagtgtt gtttacgatg caatcgtaat
     7741 gcaacgtgcg tcattaagac aaggaacaca cgcggttaaa aaccgtagcg ctgttcgcgg
     7801 cggcggacgt aaaccatggc gtcaaaaagg aacaggtcgt gcacgtcaag gatctatccg
     7861 ctcaccacaa tggcgtggag gtggcgtagt ctttggacca acaccacgtt cttacagcta
     7921 caaacttcct aaaaaagttc gtcgtttagc aatgaaatct gtattgtcag aaaaagttgc
     7981 tgaaaacaac ttggtagcaa tcgaaggctt gaacttcgac gcaccaaaaa caaaagaatt
     8041 caaacaagtt cttgctaact tatctatcga ctctaaagta ttggttgttc tagaaaaagg
     8101 taacgacttt gctgctttat cagcacgtaa cttaccaaac gtttctgtag taacttctga
     8161 taatgtgagt gtgctagacg ttgtatcaaa tacaaaagtg ttagcaacac aaactgctct
     8221 tactcaaatt gaggaggtgc ttgcataatg aacttactag acgtaatcaa acgcccagtg
     8281 atcactgaaa aatcaatgct tgccatggat gacaagaaat acacttttga agtggacact
     8341 cgcgcaaaca aaactttagt aaaacaagcg gtagaagctg cttttgacgt taaagtcaaa
     8401 aacgtgaaca tcgtgaacgt acgtgcgaaa ttcaaacgta tgggcaaata tgcaggatat
     8461 acaaaaaaac gtcgcaaagc gatcgttaca ttgactgaag attcaaaaga aatccaatta
     8521 ttcgaagctg ctgaataagc actaatagat aactcattaa attaggaggg aaaaacgtgg
     8581 cgattaaaaa gtacaaacct acctcaaatg gtcgtcgtaa catgacaggt tctgattttg
     8641 ctgaaatcac aacatcaaca cctgaaaaat cattgttaca accattgaaa aaccatgctg
     8701 gacgtaacaa caacggccgc atcactgtac gtcatcaagg tggcggacac aaacgccaat
     8761 atcgcgtgat cgacttcaaa cgtaacaaag ataacgttgt tgcaactgtt caaacgatcg
     8821 aatatgatcc aaaccgttca gctaacatcg cgttagtaca ctatgaagat ggagtgaaag
     8881 cttacatctt agcacctaaa ggactagaag tgggtatgaa acttgtttca ggtccagaag
     8941 cagatatcaa agtcggaaat gctcttccat tagaaaacat tccagttggt actgttatcc
     9001 acaacattga aatgaaacct ggtaaaggtg gacaattgat ccgttctgct ggtacaagtg
     9061 ctcaagtact tggtaaagaa ggcaaatacg tattgatccg cttgaactct ggtgaagttc
     9121 gtatgatctt agctgcatgc cgtgctacaa tcggttctgt aggtaacgaa caacacgaat
     9181 taatcaacat cggtaaagct ggacgctctc gttggatgcg taaacgccca actgtacgtg
     9241 gtagcgtaat gaaccctaat gatcacccac acggtggtgg tgaaggtaaa gctcctatcg
     9301 gacgtaaagc accagtttct ccttggggtc aaccagctct tggttacaaa actcgtaaca
     9361 aaaaagctaa atcagacaaa cttattgttc gtcgtcgcaa aaataaataa ttactttgac
     9421 catgtgtcgc acattttgag aggaggttca ccatgggtcg tagtttaaag aaaggacctt
     9481 ttgtcgatga gcatttaatg aaaaaagttg aagctcaagc aggggttgaa aagaaaaaag
     9541 ttatcaaaac ttggtcacgc cgttcaacag ttttcccaag ttttgttgga tataccatcg
     9601 cagtttatga tggacgtaaa catgtaccag tttacatcca agaagacatg gtaggacaca
     9661 aattaggtga atttgcacct acaagaactt atcgtggaca tgctgctgac gataagaaaa
     9721 cgaaacgcta atttgagggg aggataaatc caaatggcag aacaaatcac atcagctaaa
     9781 gcaactgcaa aaacagttcg cacttcacct cgaaaagccc gtttagtaat tgaccttatc
     9841 agaggtaaaa gtgtcgcaga tgcaatttca atcttgaaat tcacaccaaa caaatctgct
     9901 ggaatcattg aaaaagtatt gatgtcagca gttgcgaatg cagaaaataa ctttgactta
     9961 gatgttgaaa acttggtagt aagcgaagct tttgttaacg aaggacctac aatgaaacgt
    10021 ttccgtcctc gtgcaaaagg atcagcttct ccaatcaaca aacgtacaag tcatatcacg
    10081 gtagtcgtat cagaaaaata aggagggata atcagtgggt caaaaagtac atccaattgg
    10141 aatgcgtgta ggcatcatcc gcgactggga tgctaaatgg tatgctgaaa aagagtatgc
    10201 tgagttctta cacgaagatt taagaatccg taaatttatt tcaactaaac ttgctgatgc
    10261 tgctgtatca acagttgaaa tcgaacgcgc aacaggtcgc gtaaacattt caattcacac
    10321 agctaaacca ggtatggtta ttggtaaagg cggttctgaa gtcgaaagcc taagaaaaga
    10381 attaaacaaa ttaactggta aaagaatcca tatcaatatc gtggaaatca aaaaaccaga
    10441 tctagatgca aaattagtag gtgaaggaat cgcacgtcaa ttagaaaacc gtgttgcttt
    10501 ccgtcgcgct caaaaacaag ccatccaacg ctcaatgcgt gctggcgctc aagggatcaa
    10561 aactcaagta tctggtcgtc taaacggagc agatatcgct cgttcagaag gatactcaga
    10621 aggaactgtt cctcttcata ctttacgtgc ggacattgat tacgcatggg aagaagcaga
    10681 tacaacatac ggaaaactag gagttaaagt gtggatctat cgtggagaaa ttcttccaac
    10741 taaaaaaaac actgagaaag gagggaaata atcatgttag tacctaaacg tgtaaaacac
    10801 cgtcgtgaat tccgcggaaa aatgcgcggg gaagctaaag gcggaaaaga agtagcattc
    10861 ggtgaatacg gtttgcaagc tgttgattca cattggatca caaaccgcca aatcgaagct
    10921 gctcgtatcg caatgactcg ttacatgaaa cgtggtggga aagtatggat taaaattttc
    10981 cctcacaaat cttatactgc caaagcaatt ggggtacgta tgggttctgg taaaggggca
    11041 cctgaaggat gggttgcacc agtaaaacgt ggtaaaatca tgtttgaaat cgcaggcgtt
    11101 cctgaagaag tagctcgtga agcgttacgt ctagcttctc acaaattacc aatgaaaact
    11161 aagatcgtaa aacgtgagga aatgggtggt gaatcgaatg aaggttaaag aaatcagaga
    11221 attaaccact gccgaaatgc ttgatcaaga aaaacaatta aaagaagaat tgtttaatct
    11281 tagattccaa ttagcaacag gtcaattaga aaacactgca cgtattaaag aagtacgtaa
    11341 atcgattgca cgcatcaaaa ctgtgttgcg tgaacaagct aagtaattat ggaaggaggc
    11401 cattaagcgt atgactgaag gaagaaatca acgcaaagtt tatcaaggtc gcgtggtttc
    11461 agacaaaatg gataaaacaa ttaccgttgc tgtcgaaaca aagaaaaacc accctatcta
    11521 tggcaaacgc atgaactatt ctaagaaata caaagtgcat gatgaaaaca acacagcaaa
    11581 agttggcgac atcgtgagaa ttatggaaac tcgtccatta tcagctacaa aacgtttccg
    11641 tttgttggaa gtagtcgaag aagcagttat tatctaataa cacgagattt tcctatatag
    11701 attgaatgca aaatctgaaa ggaggataca caacgtgatc caagcagaaa gtcgtttaaa
    11761 agtcgctgat aactcaggcg cacgtgaaat tttgacgatc aaagtccttg gtggatctgg
    11821 tcgtaaaact gctaacatcg gtgatgtgat cgttgctacg gttaaacaag caacgccagg
    11881 tggggttgtc aaaaaaggtg acgtcgttaa agccgttatc gttcgaacaa aatcaggagc
    11941 tcgtcgtaca gacggttcat atattaaatt tgatgaaaat gctgcagtga ttattcgtga
    12001 tgataaaagc cctcgtggaa cacgtatctt tgggccagtt gcacgtgaac tacgtgaaaa
    12061 caacttcatg aagatcgttt cactagcacc agaagtttta taatcttgag acacttatca
    12121 aaggaggtgc gaaacagtta tgtttgttaa aaaaggcgat aaagttaaag ttatcactgg
    12181 aaaagacaaa aacaaagaag gcgttgtact agcagcgttc ccgaaaaaag ataaagttat
    12241 tgtcgaaggt gtaaatgtcg ttaaaaaaca ccaaaaacct tctcaagctg ctcctcaagg
    12301 tggaattgtt gagatggaag cgccaattca tgtttctaac gtgatggtgg ttgactctac
    12361 tggtgtagct ggccgagttg gctacaaaga agtagacggc aaaaaagtcc gtgtttctaa
    12421 aaaaaccggt gaagttttag ataaataaaa gtttaaggaa ggagggtctt actgaatgaa
    12481 ccgcctaaaa gaaaaatatc ttaaagaagt aactccatca ttgatggaaa aatttaacta
    12541 tagctctgtt atgcaaacac ctaaagttga aaaaatcgtc atcaacatgg gtgttggtga
    12601 tgcggtatca aacgctaaaa acttagataa agcagttgaa gaattaactt tgatcactgg
    12661 tcaaaaacca atgattacaa aagctaaaaa atcaatcgct ggattccgtt tacgtgaagg
    12721 aatgccaatt ggtgcaaaag ttacattgcg cggagaaaga atgtacgaat tcttagacaa
    12781 attggtttct gtttcactac cacgtgtacg tgacttccat ggtgtaagta aaaaagcctt
    12841 tgacggtcgt ggaaactata ctttaggtat taaagaacaa ttgatcttcc cagaagtaga
    12901 ttatgattta gtagataaag tacgtggtat ggacatcgtt attgtaacaa cagcaaactc
    12961 tgatgaagaa tctcgtgagt tgttgacaca attaggcatg ccattccaaa aataattaaa
    13021 ggaggcgaac tacgtggcta aaaaatcaat gattgctaaa aacaaacgtc ctgcaaaatt
    13081 ttcaacacaa gcttatactc gttgcgaacg ttgcggacgt ccacattcag tttatcgtaa
    13141 gttccatctt tgccgtattt gcttccgcga acttgcctat aaaggtcaaa ttcccggcgt
    13201 gaagaaagct agctggtaaa aagtaaattc gcataagaag gaggtaaaag atcaatggtc
    13261 atgacagatc caattgcaga ttttctaact cgtatccgta acgcaaacat ggttaaacat
    13321 gagtctttag aagtacctgc atcaaaaatt aaacgtgaca tcgctgaaat cttgaaacgt
    13381 gaaggtttcg ttcgtgatgt tgaatacatc gaagatgaca aacaaggcgt gatccgtgtt
    13441 ttcctaaaat ttggtaaaaa cggcgaacgt gttatcacaa acttaaaacg tatctctaaa
    13501 ccaggtttgc gtgcttatgt caaagctgac gaagtaccaa aagtattgaa cggtttaggt
    13561 atcgcaatca tttcgacttc tgaaggtgtt gtcactgata aagaagctcg tgaaaaaaat
    13621 atcggcggcg aagttatcgc ttacgtatgg taatcaagta gaatctacaa ggaggtgtct
    13681 ttaagtgagc cgtatcggaa ataaagtcgt tgttatccct gaaggagtaa cagttactca
    13741 agacggaaac aacgttacag ttaaaggacc aaaaggtgaa ttgactcgta ctttttcagc
    13801 agatatcaaa atgaatatcg aaggaaacga agtaactttc actcgtccaa acgacagtaa
    13861 agaaatgaaa acaattcatg gaacaactcg tgcgaacttc aacaacatgg ttgtaggtgt
    13921 cagcgaaggt ttccaaaaag cgttagaact aatcggggtt gggtaccgtg cacaattaca
    13981 aggatcaaaa cttgtattga acgttggtta ctcacatcca gtagaaatca caccaccagc
    14041 tggagtaact gttgaagtac cagctaacac acaagtaatt gttaaaggtg cgaacaaaga
    14101 agaagttggc gaattagctg caaacattcg tggaactcgt cctccagaac cttataaagg
    14161 taaaggtatt cgttatgttg gcgaatatgt acgccgtaaa gaaggtaaaa ctggtaaata
    14221 atcgtttaga aaaattgcat agaagccagc agcaggaaaa aggcatttgg ctgaagttta
    14281 ccttcaaaaa aatacatttt tacttgtgcc gcagcttcta tttttcgaaa cgtatttact
    14341 gcttgaaata gtagtacaat aaaaataaag aggtgacaat tgtgattaca aaaccagata
    14401 agaacaaaac acgtcaaaag agacatcgtc gtgtacgtaa caatatctct ggtactgctg
    14461 agcgcccacg cttgaacgtt tttcgttcta acaaaaacat ctacgctcaa attattgatg
    14521 acgtagcggg tgtaacgcta gcaagtgcct ctaccttgga taaagaaatt tcaggtggaa
    14581 caaaaacaga agcagcacaa gctgtcggta aactagttgc tgaacgtgca gctgaaaaag
    14641 gcattaaagt agtagtcttt gaccgtggtg gataccttta ccatggccgt gtgcaagctt
    14701 tagctgaagc tgctcgcgaa aatggactag aattttagaa gaaggaggaa taccattcat
    14761 ggtttatatc gatccaaaac atttggaatt agaagaccgc gttgttgcaa ttaaccgtgt
    14821 aacaaaagtt gttaaaggtg gacgtcgtct acgttttgct gctttagttg ttgtcggtga
    14881 caaaaacgga cacgtagggt ttggtacagg taaagctcaa gaagttcctg aagcaatccg
    14941 taaagcaatt gaagatgcga agaaaaactt agttgaagta cctatggttg gttcaacaat
    15001 cccacacgaa gtaatcggtg cttttagtgg tggtagaatc ctaatgaaac ctgctgtaga
    15061 aggttctggg gttgctgctg gtggaccagt tcgtgccgta ttagaattag ctggggtagc
    15121 agatatcact tctaaatctt taggctcaaa tacaccaatc aacgttgttc gcgcaacagt
    15181 tgaaggtttg aaacaattga aacgtgcaga agaagttgca gaacttcgcg gcaaatcagt
    15241 tgaagaatta atcggataag gaggacaaca ataatggctg aattaaaagt tactttaaaa
    15301 cgcagtatta tcggacgtcc tcaaaaccaa cgcgatacag ttaaagcgtt aggtcttaca
    15361 aagattaaca gttcagtggt taagccttct aatgaagcaa ttaaaggcat gatcaatact
    15421 gtttctcacc tagtggacgt tgaagaaatt taatttagaa aaatcataac tagaacatta
    15481 aggaggtgcc aaacctatga aacttcatga attaaaacct gctgaaggtt cacgccaagt
    15541 acgtaaccgt gttggtcgtg gtacttcatc tggtaacgga aaaactgccg gacgcggaca
    15601 aaaaggacaa aaagctcgtt caggcggcgg tgtgcgttta ggatttgaag ggggacaaac
    15661 accattgttc cgtcgtttac caaaacgagg attcacaaac gtgaaccgta aagactatgc
    15721 agtcattaat cttgatgtat tgaaccgctt tgaagacgga gctgaagtaa ctcctgttgc
    15781 tttagttgaa gctggaatcg tgaaaaacga aaaagctgga atcaaagtat tagctaatgg
    15841 ggaactaaac aagaaattaa ctgtgaaagc agcaaaattc tctaaagcag ctcaagaagc
    15901 aattgaagcg gctggtggat caatcgaggt gatctaatgt ttagactatt aaaagatgct
    15961 tttaaagtaa aagacattag atcgaaaatc ctatttactg tatttgtgtt gtttgtattt
    16021 cgcctaggtg cacatattac tgtaccaggc gtaaatgctg cttcattgca aaacctttct
    16081 tctttgccat ttttcaacat gttgaacttg gtaagtggta gtgcgatgca aaacttttcc
    16141 attttctcaa tgggggtttc accatacatt actgcttcta tcgtcattca gcttcttcaa
    16201 atggacatcg tgcctcgatt tgttgaatgg tcgaaacaag gggaagttgg tcgtaaaaaa
    16261 ctgaatcaag cgactcgata tctgacatta gtattggcat ttatccaatc tgtcggtatc
    16321 actgctggat tcaatcaata cgctcaatta ggtttcgtga ataatcctaa cgtagcaact
    16381 tacgtaacga tcggtttgat tctaacagcc ggtacgatgt ttgttacttg gctaggagaa
    16441 cagatcacag aaaaaggtat cggaaatggc gtatcaatga tcatttttgc aggtatcatt
    16501 tctagattgc ctgaaagtat cagagaaatt tatgaagatt actttataaa tattgatcct
    16561 tcagacatat ggaaatctgc tatttttgtg gccattttac tagtagcggt cttgttgatc
    16621 gtgacattcg taacattttt ccaacaagct gaacgcaaga tcccgatcca atatacgaaa
    16681 cgcgtagctg gcgcgcctac aagcagctat ctgccgttga aagtaaatgc agctggagtt
    16741 attccagtta tctttgctag ttcgtttatt gctacgccaa atgctgtttt acaagcattg
    16801 agcggtagat tcggttctga aaactggtac aaagtgatga tggagatctt taactataat
    16861 acagcacctg gtgctacgat ttatacgatt ctgatcgttg ccttcacttt cttctacgcc
    16921 tttgttcaag taaaccctga gaaattagcg gaaaacttgc aaaagcaagg aagttatata
    16981 ccaagcgtac gccctggtaa ggggactgaa gagtatgtct caagattact gatgcgttta
    17041 agtacagttg gtgcagtctt ccttggttta gtggcattgc ttccaatcat tgctcaaatg
    17101 gtttgggatc ttccacaatc aatcggatta ggcggtacaa gcttactgat cgttatcggt
    17161 gtagctttag aaactgcgaa acagttagaa ggcttaatgc tgaaacgtaa atatgttgga
    17221 ttcattgagt aagaaagcgt gttgaggttt cccctcaaca cacgttctca cacatttatt
    17281 aggagggaaa acaatgaacc tcattttaat ggggctgcca ggcgcaggta aaggaacaca
    17341 agctgaaaaa atcattgatg cttatggtat tccgcacatc tcaacaggag atatgttccg
    17401 cgcagcgatg caaaacgaaa cagctcttgg cctggaagca aagtcttata tggataaagg
    17461 tgcattagtt cctgatgaag taacaaatgg aatcgtgaaa gagcgactag ctgaaccaga
    17521 tacagagaaa ggcttcttat tagatggctt tccacgtacg ctagatcaag caaaagctct
    17581 ggacgcaatg ctaaaagatt tgaataaaaa aattgatgct gtcatcgata tccatgttgg
    17641 tgaagaaatc ttggtcgaac gtctagccgg ccgatttatc tgcagcaatt gtggagcaac
    17701 ttaccataaa attttcaacc ctacaaaagt tgaagacaca tgtgatcgtt gcggtgggca
    17761 tgaattctat caaagagaag atgataaacc tgagacggtt aaaaatcgtc tggctatcaa
    17821 catcaaaaat agtgaaccaa tcttggctta ttataaagaa caaggtttgc taaacactat
    17881 tgatggtgct cgtgaaatcg atgctgtatt tgcagatgta caaaaaatca ttgaaaaata
    17941 gcacgattgc ccgtagccga cttgcattat gcagttactc ttgtcaagtt caataaatta
    18001 tgatagaata tgtcagtccg acttctagag aatcctctag atgatccatt ttgagaattt
    18061 tgaggtggga actgcagttt ccgccatttt atcgtgtgaa aatgcgaaac aagtacaagg
    18121 aggtactgtg cgtggcaaaa gaagatatga ttgaagtcga aggtacagtc gtcgaaactt
    18181 tgccgaatgc aatgtttaaa gttgaactag aaaacggaca ccaagttctt gctactgttt
    18241 caggtaaaat tcgtatgcat tacatccgta tcttacctgg tgacaaagta acggttgaat
    18301 tgtctccata cgatcttaac cgtggccgta ttacttatcg ctttaaataa ttgtactccg
    18361 taaaactaca ggaggtatta tcatgaaagt aagaccatca gtaaaaccaa tgtgcgaaca
    18421 ttgtaaagta attcgtcgta aaggacgcgt tatggtgatt tgcccagcaa atccaaaaca
    18481 taaacaacgt caaggataat tggaggtgct ataaatatgg ctcgtattgc aggagtagat
    18541 attcctcgtg ataaacgtgt agtagtttca cttacttata tctatggtat tggtaacact
    18601 actgctaaaa agatcttagc tgatgctggt gtatctgaag atgttcgcgt tcgtgaatta
    18661 acgaatgaac aaacagatgc tatccgtgca gaagtagata aattaaaagt tgaaggggat
    18721 cttcgtcgtg aagtgaactt gaacatcaaa cgtttgatgg aaatcggttc ttaccgtgga
    18781 atccgtcatc gccgtggatt gcctgttcgt ggacaaaaca cgaaaaacaa tgcacgtact
    18841 cgtaaaggcc cagctcgcgc tatcgctggc aagaaaaaat aattcttaag tgaaggaggt
    18901 taaaacttca tggcagcaaa aaaagtgaat cgtaaacgcc gtgtgaagaa gaatatagaa
    18961 gcaggtattg cacatatcca ttctacattc aataacacta tcatcatgat cactgatact
    19021 cacggaaatg cattagcgtg gtcatcagct ggttcattag gtttcaaagg aagcaaaaaa
    19081 tcaactcctt ttgccgctca aatggcagca gaaactgcta caaaagcagc aatggaacac
    19141 ggattaaaga ctgttgaagt aacagttaaa ggacctggtt ctggacgtga agcagcaatt
    19201 cgttcattac aagctacagg tttagaagtg actgcaattc gtgacgtgac tccagttcct
    19261 cataatggtg cccgccctcc aaaacgccgt cgtgtttaat gagtgccgct cattttgagt
    19321 tttatcagta acgtcattga acaagacact tttcgttttg aaaggggtaa agataagaat
    19381 gattgagttc gaaaaaccaa gaatcgcaaa aattgatgaa gaaaaagatt atggcaagtt
    19441 catcgttgaa cctcttgaaa gagggtatgg gactacttta gggaattccc tacgtcgtat
    19501 tttattatct tctttgccag gtgctgccat cactaatatt caaatcgatg gtgtactgca
    19561 tgaattctct accatcaaag gtgttagaga agacgtcact caaatcatct tgaacattaa
    19621 aggcttagcg ctgaaaatgt atggccaaga agaaaaaacc cttgaaatcg atattaccgg
    19681 acctgctaca gtaactgctg gcgatattat cgttgatagt gatgtagaaa ttcttaacaa
    19741 agatatgtac atctgtagtg tctctgaagg agctacgttc catgctcgct taacagtgaa
    19801 acctggacgt ggttatgttc aagcagatga aaataaaaaa gaagatatgc caattggcgt
    19861 tcttccagtc gattcaatct acacacctgt tcgtcgtgtg aactatcaag ttgagaacac
    19921 acgtgttgga caccgtgaag attttgataa attgacgatg gagatttgga cagatggttc
    19981 aattgaaccg ttggaagcaa tgagtttgtc tgcgaaaatc atgactgaac atttggatat
    20041 ttttgtgaac ttaactgatg aagcaaaaaa tgctgaaatc atggtcgaaa aagaagaaac
    20101 acaaaaagaa aaaatgttgg aaatgacgat cgaagagtta gacttgtctg ttcgctctta
    20161 caattgtttg aaacgtgcag gaatcaatac tgtacaagaa cttacaaata aatctgagcc
    20221 tgaaatgatc aaagtccgta acttaggacg taaatcatta gaagaagtta aagcaaaatt
    20281 gcacgactta ggtttaggtt tacgtaaaga agattaaaac cttttacgaa ggagggaaac
    20341 caacgtgagt tatcgtaaac taggacgcac atctagccaa cgtaaagcga tgttgcgtga
    20401 tttaacaact gatttattaa tcaacgaacg tattgtgact actgaagctc gtgcgaaaga
    20461 agttcgctca actgcagaaa aaatgatcac tttaggtaaa cgcggagatt tgcatgcacg
    20521 tcgtcaagct gcggctttcg tacgtaatga agttgccagc gtgcgtgaag aaaatgagga
    20581 catcgtcatt gaatcagctt tacaaaaact gttcaacgat attgcacctc gttacgcaga
    20641 acgtcaaggc ggatacacac gcattttgaa gacagaacca agacgcggtg atggtgcacc
    20701 aatggttgtc ttagaacttg tctaaaatca cactaataac atcactttat agatgattct
    20761 tttagagtgt tatgatgttg gaagatattt ttccgagtct agctcagcgc tgctcctcaa
    20821 gcgaatatcg cttggggagt aagcactcat cataaagtgt ttttttatac cttcaattac
    20881 ctgtcatgac agatatgttt gaaggcataa aaaaacattt taagcgagaa ataattgaaa
    20941 attttgtctt tttttgatag tgttagtagg aactttagga tgaagaagga gtaatcatgg
    21001 agccaattat tgaattagaa aagataaatt ataaatacca gccagatgat cttcgccccg
    21061 cattgaaaga tgtctccttt acaattgata aaggtgagtg gatcgctatt attggacaca
    21121 atggttcagg aaaatcgaca cttgccaaaa cgatcaatgg attgcttctg ccagaatcag
    21181 ggatcgtgaa agtgggaaat caaatattgg atgaagaaaa tatttggacg atccgacaga
    21241 tggttggaat gatttttcaa aatccagaca atcagtttgt tggttcgaca gtagaagatg
    21301 acgttgcttt tggtcttgaa aatcaaggga ttcccagaga agaaatgcta gtgcgtgtga
    21361 aagatgcact tgagaaagtt agaatggctg agtttgcttc acgcgagcct gctcgtttat
    21421 caggagggca aaaacaacgt gttgccattg ccggcgtggt tgccttacga cctgatatca
    21481 ttattttaga tgaagcaacc agtatgctag atccagaagg tcgtgaagaa gtgatctcta
    21541 cgatcaagaa gatcaaagaa gaaagtcaat tgaccgttat atctatcact catgatattg
    21601 atgaagcagc taatgccaat cggatactag tgatgagaca aggcgaactt gtccgtgaag
    21661 gtacaccaaa agaaattttt tctgctggtc ctgaactgat cgacttaggt ctagatctac
    21721 catttcctga aaaactgaag agcgcactga aagaacgtgg ggttgatgtg cccagtgaat
    21781 atatgacaga agaaaggatg gtggattggt tatggacatc cgttttgaac aagtagactt
    21841 cacttatcag ccgaatacac catttgaaca aagagcctta tttgacatca atatgacgat
    21901 caaagaaaac agttatactg cattagtagg gcataccgga agcgggaaat caaccttact
    21961 tcagcatttg aatgcgcttg ttaagccaac aagcggtaca gtccatattg gagaacgaga
    22021 tattcagccg gatacggata ataagaattt aaagcccatc cgaaaaaaag ttggcatcgt
    22081 ctttcagttt ccagaagcac agctattcga agaaacggta gcaaaagaca ttgctttcgg
    22141 tcctaaaaac tttggtgtca gcgaagaaga agcactagtc ctagcaaaag aaacattaga
    22201 acaagttggg ctggatgaaa gctatttgga acgttcgcca tttgaacttt caggagggca
    22261 aatgcgtaga gtagcaatcg ctggtgtgct tgccatgagg ccggaagtac ttgtattgga
    22321 tgaaccaacg gcaggacttg atccccaggg cagaaaagaa atgatggaga tgttttggcg
    22381 tcttcataag gaacagcaga tcactattgt attagttacg catttaatgg atgatgtagc
    22441 caattttgca gattatgtct atgtgttgga aaagggacga atcgttaata gcggggagcc
    22501 gcaagaggtc tttcaaaaca ttgaatggct aaaagaaaaa caactaggcg tacctactgc
    22561 caccgagttt gcagaagaat taatggctaa agggatgaac tttgctacct tgcctttgac
    22621 agctgaagaa ttagcagacg ctatcgtcca ttatgctgga ggaaatgccc atgatgaata
    22681 aattgatatt tggtcgttat atgcctggag attccttgat ccatcgattg gatccccgag
    22741 ctaaattatt agccagtttt tattttatcg gaatcatttt tttagcaaat aattgggaaa
    22801 cgtacctttt gttggcattt ttcacattac tttctgtctt cttgtcaaaa gtagatttga
    22861 aattttttat ccgaggcatt cgcccattga tctggctaat cctcttcact gtagctttgc
    22921 aggtcttgtt tacaagtggc ggagaaactt attggagttg gggtgttttc catatcactg
    22981 aatttggtgt gatgaacggc ctctttattt tctgtcgatt cgtgttgatc atttttatgt
    23041 cgactctact cacgcttact acaccgcctt tggaattatc ggatgcgatt gagtacttgc
    23101 ttcgtccatt gaaagccgta cggttcccag tccacgaaat ttcattgatg ctgtcgattg
    23161 ctcttcgttt tgtgcctaca ttgatggacg aaacagaaaa gatcatgaac gctcagcgcg
    23221 ctcgcggtgt tgattttggt gaaggaaacc tagttcagaa aatgaaagca gtcattcctt
    23281 tgctgattcc tttatttgtc agcagcttca accgtgcgga agatcttgcg actgctatgg
    23341 aagcaagagg ctacaaaggt ggggaaggaa gaacgaaata tcgtgtattg cattggcata
    23401 atagagacac gatggttatc attgcttttg tattattaac tgtgctactg atctttttaa
    23461 gaggataaac gttcagtggg ccgctatcta ttcttttctt gctgatctgt ttgcaagaaa
    23521 ggatgcaagc agtgtatgac aagaaatatt agggtctgtg gcagactaag gtcacaggcc
    23581 tctttctttt atctgaaaag aaagaacgaa aaaattttat agaaaaatca ttattccaga
    23641 taacctttta ggaacaaagg agagaaaaac atggtacgtt acaaagcaat cattgcgtac
    23701 gacggaacaa attttaatgg attccaaaaa cagccaaatg ggcgtactgt tcaagaagaa
    23761 gtagaaaaaa cattacaaaa aatggcaaat ggaaaagaaa tcacggtctt tggttcggga
    23821 aggacagatg ccggagtaca tgcgatcggg caagtaatcc attttgatta tccagaggag
    23881 cgtccgttag aacgaatgcg tttcgcgcta gatacccagt caccagagga tattgctgta
    23941 agacaagtag aaattgtttc agaagacttc cacgcaagat atttggtcaa agaaaagacg
    24001 tatcaatttc gagtggatat agggaaacca cgcagtcctt ttcgacgcca ttatgcaagt
    24061 tattttcctt acccacttga tttggaaaaa attcagcggg cgcttcctga tttattaggg
    24121 actcacgatt tcacctcttt ttgtgcatca ggaagctcga tagaggataa aatacgtacg
    24181 atctacgagg ctaagatgga ggtaaatgaa acaaaagacg aattgttgtt tacatttagg
    24241 ggaaatggct tcttgtataa gatgatacgg atcttagtag gaacgctttt gaaaattggc
    24301 aatggccgat tgcctgaaga tagtatccca gatattattg cgaaaaaaga tcgtaatgca
    24361 gcaggaccta cagcacaccc tgaaggactt tatttgtatg aagtgaaata tgaataagag
    24421 gttatgatag aagtggtcag cttcaagaaa taagaaggta tttacgaaaa ttacttctca
    24481 aaattttgca aaatttcggc ttattttcga agaagctact tctggaacac cgtttaagaa
    24541 agaggtcgtg agcctgtcat ttaactctga gtcacaaaga gcaatttcaa aaaacagctt
    24601 ctcatctttg atgaatatcg tgacttgtgg acgaggcgtt gataagtgaa tcatcttttt
    24661 gttacctatt ttttctgtaa ccaaaaaacg atggaacgtt gttttgattg cccttggtgc
    24721 ggacagtctg aacaggttcc atcgtttctt tattttctac gtcttcttct gtgtgtgttt
    24781 cgtggttgtt cttctattgc tggatcaatc gtgccataaa aggggcgatt gcggtcaagg
    24841 taccagacat aaagaataaa caatgcaata cataggacaa aaaggattgc acctatcgta
    24901 aatccttcag gcaagtaact gaaagtgaca gtatgtttac ctgctggaac ttttatcatc
    24961 aagaatgcgt ctttgaaatt gctgaccttg actggtttcc catcaatttt agctttccat
    25021 cctttgtcat aaggaatagt tgtcaccaac atggaatctt cagcggtatt tacagtccca
    25081 gaagcttttc tgccagtggc ttccatttcg acaccttttt ctttgatctg attcattgcg
    25141 gcttgatagg ctacaacatc tagaccaacg actttaggct ccatgaaact tatttctttc
    25201 gtcccataaa aacttgcagt aaatgtcact gttgtctctt gaggataata gccaagatca
    25261 tagtattgac cagaaatgtt gatttgcgtt tttcgcgatg taccattgac agtgatcgtc
    25321 gcactagaac tttctaattg cgcaaaattg gttgggaaca aacttaagta agcttgtgta
    25381 tttgcaggca catggacttc ccaagtgagc tcttttggct ggttatctgc tgtttctgag
    25441 taagtgacgc cagtcgttgt ctttgtgatc gtcgtattca actgattctt caaagtaggt
    25501 gtatagaatg taaaatactg ttcatttaga ccagacaatg tattgatcaa gtttgtctga
    25561 ctggtcaaat tatctgtcac tggctgctca acatcattaa cggacaacgg tgcagtaaat
    25621 gctagtggca atgcattgac attctgatac agtgtatagt cgccgctttg accagcttgg
    25681 cggaagccat atttactgaa cgtgctttcg tttttagaaa tgttatattt gattcctgta
    25741 aacccatcca tcagcaaagt attattgtta taacggatat tcaaatttgt ccctcttgaa
    25801 cggaaaccta atttatccag gtaagaggaa gaattacgat ttcttataga agaaaacaaa
    25861 ctgataccac tgtaaccata gttgatcgaa tcgtttgcag atactggatc cagattttcc
    25921 aagcggtaaa acgagtcgga atcttttttt gtttcattta caagagcttt caagtcagga
    25981 taaggatcag tatataaact gcgggaagca taattccaat catcaagaat gccatggatc
    26041 atactgttcg tattgaccag tgcttcagca gacatgatca ataataacaa tatactcaga
    26101 tgattgaatg aaagtttttt atgctgataa aatcccacag caaaagcata aatccctaag
    26161 aaaaccactg tcaatacaaa ggaagtcatc gtcacatagg tgtagcttcc ttcaggtttg
    26221 atgccccatg ccaccgcaaa gagagcagct acgaccaaaa tgattcctaa caacagccca
    26281 ccatcgtctt gttctatctt ttcccatcct tttcctgcaa gcaggagcac taagaaagag
    26341 aacagatagg cataacggaa taaaaacata ttcggtgcat gcataccatg ccagaacaaa
    26401 ttcaatggaa caagataaaa acttgcgata agtatggcga aaagactgcc gtacaaaaat
    26461 ttatttttca acggtacttt ccgactggca aaataaaaca gacagaggac taatggtagc
    26521 agacctgcgt aaacgaacgg aatcgatccg tattttgtcg tgtcataaac accaatcata
    26581 tttttcatga ttagatccag ataagaagtc gcttctgttt tcaacgtagt gatctgactc
    26641 agtgcctcgc catttgaccg aagatccagc actgctggca atacaatgat cattgaagcg
    26701 ccgcctgcca gtaaagaagt gataccataa ggaacaatcg tttttttata ttctgaccag
    26761 tttgtcaata gacgggcgat aaaatataag aaagaaaata cgccaatcat aaaacccata
    26821 taaaaactgg ataaaaacaa gagtaaataa ctgataaata aaagtttcgg ccgtttaaga
    26881 tccataacac gatgaatccc caaaatcact aatggaagat aaacaaatgc atccagccac
    26941 atgatgatct ccgaatgtgc agtgataaat gagatcaatg cgtaactgac gctaagtgca
    27001 acatgccccc agcgaggaat cttgaacgta cgtgcaagaa accaaaaagc caatccggca
    27061 cagccgacct taagcaaagt cagaacatac agtgcatctg gcatcatctg gtttggaaaa
    27121 aagaacacca gtggcgtgaa gatcccgcct agatagtaag aaattaaaga taaataattc
    27181 aatcctagag aagcattcca tgtataaaaa atactttgct ttccaagaag aacatttcgg
    27241 aagctggcat gaaaattaga aaactgtgaa aaggcatcac ttgccaaaat acttctgctg
    27301 cttccaggat agattccgat acttagataa acaagtgcta aaatcaaaaa cggaatcagg
    27361 aaactagctg ctgcgtagcg tccgttttct ctaaagaatg actttaaatt ttttttcata
    27421 tttattttcc accaaatttc tatgaagttg tattcagttt atcatatcct tgtttttctc
    27481 tgcttaaaaa gagtctagtt ttcacaaaaa aataaaattt agaataaata acttcataat
    27541 ttgttcgtat aacaaaaata aacgcaaaaa aagggtagga a
//
LOCUS       DAYYCY010000018        27017 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000018 DAYYCY010000000
VERSION     DAYYCY010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27017
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.018"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            293..1462
                     /locus_tag="ACHAZB_000649"
     CDS             293..1462
                     /locus_tag="ACHAZB_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFM8613349.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
     gene            1935..2489
                     /locus_tag="ACHAZB_000650"
     CDS             1935..2489
                     /locus_tag="ACHAZB_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8613350.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(2662..3135)
                     /locus_tag="ACHAZB_000651"
     CDS             complement(2662..3135)
                     /locus_tag="ACHAZB_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8613351.1"
                     /translation="MRIKPIKDGEQLLFSSEENKPLYLDYVNHNLKIIIKENNLKHIT
                     LHGFRHTHCSLLFESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFM
                     SSTEEKQGSLQKVKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            3147..4094
                     /locus_tag="ACHAZB_000652"
                     /pseudo
     CDS             3147..4094
                     /locus_tag="ACHAZB_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634498.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
     gene            4365..4800
                     /locus_tag="ACHAZB_000653"
                     /pseudo
     CDS             4365..4800
                     /locus_tag="ACHAZB_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345965.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
     gene            4816..5649
                     /locus_tag="ACHAZB_000654"
     CDS             4816..5649
                     /locus_tag="ACHAZB_000654"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8613352.1"
                     /translation="MLYFSAIDNADFVFSDPFTAEVLSGIEEQAKNHGYFVLVHNVNS
                     AEDVKTIQRNWRFEGFIVVGVRVKEFLELNNVFDGPVVYIDTHLSKETLRELEQQMNR
                     LFVNTNDYQASTLAVEHLIHYGHEKIAFLSFDYIVGEPSVIQERHQAYIDTLKKYHLT
                     FKSHWIYNNSEFERIYQELAEFTAIVVTGDYLAAKLVKFLKNKNKRVMEQLSIIGFDD
                     ITFAELMDPALTTIRLNPGNKGAIAFHQLIGLAKQEEIKNQVLLLEGELISRDSVHKI
                     K"
     gene            5894..6919
                     /locus_tag="ACHAZB_000655"
     CDS             5894..6919
                     /locus_tag="ACHAZB_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8613353.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            7057..7182
                     /locus_tag="ACHAZB_000656"
     CDS             7057..7182
                     /locus_tag="ACHAZB_000656"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8613354.1"
                     /translation="MKHFKKIALTGIAVLTGTLLAACGGGDSNSKDGQVTLSYAS"
     gene            7207..8322
                     /locus_tag="ACHAZB_000657"
                     /pseudo
     CDS             7207..8322
                     /locus_tag="ACHAZB_000657"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
     gene            8405..9301
                     /locus_tag="ACHAZB_000658"
                     /pseudo
     CDS             8405..9301
                     /locus_tag="ACHAZB_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338086.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
     gene            9317..10141
                     /locus_tag="ACHAZB_000659"
     CDS             9317..10141
                     /locus_tag="ACHAZB_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8613355.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            10173..11483
                     /locus_tag="ACHAZB_000660"
     CDS             10173..11483
                     /locus_tag="ACHAZB_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HFM8613356.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            11631..12890
                     /locus_tag="ACHAZB_000661"
     CDS             11631..12890
                     /locus_tag="ACHAZB_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM8613357.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            12900..13772
                     /locus_tag="ACHAZB_000662"
     CDS             12900..13772
                     /locus_tag="ACHAZB_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8613358.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            13784..14614
                     /locus_tag="ACHAZB_000663"
     CDS             13784..14614
                     /locus_tag="ACHAZB_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8613359.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            14654..16837
                     /locus_tag="ACHAZB_000664"
     CDS             14654..16837
                     /locus_tag="ACHAZB_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFM8613360.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            16898..18118
                     /locus_tag="ACHAZB_000665"
     CDS             16898..18118
                     /locus_tag="ACHAZB_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFM8613361.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            18115..19575
                     /gene="gtfA"
                     /locus_tag="ACHAZB_000666"
     CDS             18115..19575
                     /gene="gtfA"
                     /locus_tag="ACHAZB_000666"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HFM8613362.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            19652..20758
                     /locus_tag="ACHAZB_000667"
                     /pseudo
     CDS             19652..20758
                     /locus_tag="ACHAZB_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            21080..21289
                     /locus_tag="ACHAZB_000668"
     CDS             21080..21289
                     /locus_tag="ACHAZB_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFM8613363.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            21282..21431
                     /locus_tag="ACHAZB_000669"
     CDS             21282..21431
                     /locus_tag="ACHAZB_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFM8613364.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            21585..22250
                     /locus_tag="ACHAZB_000670"
     CDS             21585..22250
                     /locus_tag="ACHAZB_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8613365.1"
                     /translation="MELREIRPIDIQKWQNNLLSKYKNSYVRNMYGIFQMSLDRAVVL
                     GLLEKNPAKVIGNVKKRKTTVDFWTKEEFTKVISTFYIDDLYQHFGFICMWLLFMTVM
                     RLGEATALTWKDIDFENKNLRVNKTLYYKRAKTYEFVEPETASSIRTIELDDNTITFL
                     KAWKERQVSIGGMSFVLSYNGTPTQKYTIRHIMRRHATIVGVHIIRIHGLRHSHASLL
                     ISQ"
     gene            complement(22534..23619)
                     /gene="xerS"
                     /locus_tag="ACHAZB_000671"
     CDS             complement(22534..23619)
                     /gene="xerS"
                     /locus_tag="ACHAZB_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HFM8613366.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            23953..24186
                     /locus_tag="ACHAZB_000672"
     CDS             23953..24186
                     /locus_tag="ACHAZB_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HFM8613367.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            24183..24590
                     /locus_tag="ACHAZB_000673"
     CDS             24183..24590
                     /locus_tag="ACHAZB_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFM8613368.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(25727..26245)
                     /locus_tag="ACHAZB_000674"
     CDS             complement(25727..26245)
                     /locus_tag="ACHAZB_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HFM8613369.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            26612..26860
                     /locus_tag="ACHAZB_000675"
     CDS             26612..26860
                     /locus_tag="ACHAZB_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8613370.1"
                     /translation="MVKLKMLYIGLQHRSFFDCALGKKYYEVKIDDDTFLTAHQMKQL
                     QKRNDQLEENLILKKRLPYSRLARRTIKGQDLPEDHPF"
BASE COUNT         9247 a         4099 c         4995 g         8676 t
ORIGIN      
        1 ttaataatat actaccattt gttggtagta taatttaaag gtaataagtt attcttttat
       61 tactatgttt gggttttata gcaaagaaag tttaataaga gtttggacct agcaaacaat
      121 attaatgagg tcattaaatc ttattatctt tagtgatata acgaaatatc tacacagaca
      181 aaatgatagc gtaaaatatt ttgtgtaaat agatatttag ggtagaaaaa aagaaagtcc
      241 attctgtaaa attaaagtta ccacaaccga aattttatag gaggactttc tcatgcccaa
      301 ttttactaca gaaattatgg aaacactaat caataaaggt gatttagatg aattatttcg
      361 atgtcattta gaattagctg ttaattcatt attgcaggct gaattaacag cttttcttga
      421 ttatgaaaaa tatgatcgtg ctgggttcaa ttcaggtaat tcccgtaatg ggaattattc
      481 acgttcattc agaacagagt atggagaatt aaatttggta attcctagag atagaaatgg
      541 taagttttct caacaaactt tacctgctta taaaagaacc aatgactctt tagaaaccac
      601 tattattcag ctatttcaaa aaggaattac tatgtctgaa atctctgaat tgattgaaaa
      661 aatgtacggg catcactata cgccacaaac catttcaaat atgactaaaa tagtttctga
      721 agatattatt gcctttaaag aaaggtcctt agaatccaga tactctgtca tttttatgga
      781 tgctactcac attcctttaa aaagacaaac tgtttcaaaa gaagcagttt atattgtaat
      841 tggcattcga ttagatggaa ccaaagaggt tcttgggttt agtcttgctc caactgaatc
      901 tgcttatgtt tggaaagaga ttcttcaaga cctaaaagac cgtggtttaa aagaggtttt
      961 attagtcgta actgatggtt taagtggcat taacgatagt attcatagtg tttatccaaa
     1021 tgctcaattt caacaatgtt gtgtccatat atctagaaat atcgctcata aggttcgtgt
     1081 tagtgatcga caagaaatct gtagcgattt caaattagtt tatcaagcaa gttcaaaaga
     1141 agaagcaaat aatcaaataa ggtttatgat agataaatgg aaaaagcagt atccacgagt
     1201 ggttaaattg ctcatgaatc ctgctatatt aaccttttat aatttccctc cgtcaatcag
     1261 aagaactatt tattcaacta atttgattga aggttttaac aaacaattaa agaaatatac
     1321 caaaagaaaa gagcaatttc ctaatgaaga atctctggag agatttctag tatctcaatt
     1381 caacaactat aatcaaaagt ttctatgccg agttcataaa ggttttaaag aaatacatga
     1441 tacattagaa tcaatgtttt aacttgatca acagaatggg cttttccatt tacacaaaat
     1501 tattgacgct cccgacaaaa ttatgatctt gcgtaaagtt ataaaatatt aataaaaata
     1561 ttttattaat gaaaagcttg gtagcacggt tattcatatg ctaatttatt tcactttata
     1621 gtaaggggag ttcttagtat attgttcaaa cttaaatttc atataattta attatgtaaa
     1681 ttagctattc taagaacttc tacggataac tgcccgtcta aaatttaaat aagagcttag
     1741 aatcttcata taagtaaggt taattttttt gtacaaaaaa gtcggaatat tctgagttac
     1801 tgaaatgata taaaatcata aactaaatcc cttgatttta ttgactttca aaagtttata
     1861 tatataagtt aatgattatg gtaaaatata tacataatca ttaactcaga atatttttaa
     1921 gaagggaaac aatagtgagc tataatgtac aaccgttaag gacaaatgaa gagatacaag
     1981 attttcagtt ttggctaaga agaacaagta atccggaaag agatagtttt ttatttttgt
     2041 ttggaatcaa taatgggtta cgtatgtcag atattatagg attaaaagtt ggagatatac
     2101 gaggcaaaag taagcctatt attgttgaac ataaaacagg taagcgaaaa ccgattttta
     2161 tagataattt gagagaagaa attttattat atactgaagg taaagaagaa aatgattggt
     2221 tatttccgag tcggcaacaa ggtcgacata taacaaggga tcgtgtctat cagatatatg
     2281 cagatatagc agaaaaatta ggacgagatg atataggaac acatacatta agaaaaactt
     2341 ttgggtatca ctactacaag aaaactagag acattgctac cttaatgttt atttttaatc
     2401 atagctcgca agcaattaca aaaaggtaca ttggtataac tgaagatgaa ataggagcat
     2461 cacttcgtgg atttaaacta ggtgtataat ttaaaaattt ttataaacag taaagacaaa
     2521 gattaaaagc tatttttatc tagaacgatt ttatttcata aagttcgcgg aataaaagct
     2581 ggggttactg gtacttggta taggttcgtg ttatataaac taagtaatta aataacattt
     2641 ttaggttgga aaaggatgaa attaaaaaaa gagaatcaat acggtctcaa aaacgcaaac
     2701 gctgcggaat aagaaaaata ctgataactc aacgtttagc agactatcta atagggatag
     2761 tctgcttttt tcgtttttga ccatactttt gaccttctgc aaacttcctt gcttttcctc
     2821 tgtagaactc atgaaatctg caaaacgaat tgccgtttcc tctgtctgct ttttcgcgat
     2881 atgggtgtat atatcatcca tagtggtttt aatatctgta tgtccaagtc gaacatacac
     2941 ttctttgaca gacgcaccag attcaaagag cagactacaa tgggtatgac gaaaaccatg
     3001 aagtgtgata tgttttaagt tattctcttt aatgatgatt ttcaaattgt gatttacata
     3061 gtctaaataa aggggcttat tttcttctga tgaaaacaaa agttgttcac catcttttat
     3121 aggtttgatt ctcattttta gatattatgc ccgcacgtat tcatcacatt tctattgtga
     3181 atcgctttat tcgaccgaca tttgactttt atcataatat actcggttta aaattactaa
     3241 tgaaaacaat taatcaagac gatcatacca tgtatcacct gtttttttca gataatcacc
     3301 accgtgtggg aactgaactg actttttttg aagtacaaga agggaacaac cagttttttg
     3361 gaaccaatac gattgaacga acgatactaa aagttccttc cgaagcatca ttgcattttt
     3421 gggaaatata ttttgaaaca caaggcgttt gccattatgg aatcgaatca ttttctggac
     3481 gacctatctt acgttttgaa gggccagatg caacacgatt agctttagtt cccttacgcg
     3541 aatttgaaga tattgatgat tatttcccgt atgtcacaga ccagatccct acagagcatg
     3601 ccattttagg aattgatgcc attcaactac gcgtacaata ttccgatgcg actgaacgag
     3661 agttattgtc ggtaggctgg tagcacaaag aatacgtcag tttcttttcg ggtcatcaac
     3721 gagtgacaat cttagaaaat caagacactc agttctatca agaagttcag attattcaag
     3781 atcgtgagaa tccagttgcc attgaaggga ttggtagcgt ccatcatgtt gcgtttggcg
     3841 tagaaaataa atctgattta caacgcatcg acaaacaatt acaagagcgc aattttataa
     3901 attcaggaat aaaagatcgt gaattttttg tatcacttta ttatcgtgac ccaaatcaat
     3961 tattgattga aattgctaca ggggaaggaa acctagatgc aaaagcgtat gaaaatcaaa
     4021 gtccacgctt tgaagaaatt cctttatttt taccgcagta cttaaatttc attcgtgaac
     4081 aagtggagca ataattagct aatcaaatcc atgacgaaga gtaagaggtt gactcataag
     4141 tcaaaaaata aagaaaaacg catcaaatcc attctaaaga atgttgattt gatgcggttc
     4201 ctatttttat aaactatcat tttcgactaa taaaactact tatgagtcag cctctctttt
     4261 tttcttgatt tttatacctc tttctaggta aactaaacat ctgccaaaaa taccgtgtct
     4321 cattgaaaca ttctcaacta tttcaaaata aaggggagta ccaaatgaac gagtatattg
     4381 aaattatcca cgcaagagaa acaacttaaa aatgtcagtc tcaaaattcc caaaaataaa
     4441 attagtattt ttaccagcgt atctggttca ggaaaatcat cgattgtttt tgatactatt
     4501 acccaagaag ctggtcgtca actgaatgac acctatgata gttttacccg tttattttta
     4561 cctaaataca gtcgtccaga ggttgatgag attgataatc tttctacggc gattgtggtc
     4621 aatcaaaaga agattggtgg cagtgcgcgc tcaacgttag ggacgattac taacatcaat
     4681 gcgttatttc gtgtgttgtt ttcatgtttt gcaacaccta gtttaggata tgcaaatgct
     4741 tattcattta atgatcctag tggaatgtgt tctgtctgtc agggaatcgg taagacgatt
     4801 gcatttttaa taggattgct ttatttttcg gctattgata atgctgattt tgtattttct
     4861 gatccattta ccgcagaagt tttgtctgga attgaggaac aggcaaagaa tcatggttat
     4921 tttgtattgg tgcataatgt taattcagca gaagatgtta aaactattca gagaaattgg
     4981 cgttttgagg ggttcattgt tgttggtgta cgtgtaaaag aatttttgga attaaataac
     5041 gtttttgatg gtcctgttgt ttatattgat acgcatcttt caaaagagac attaagagaa
     5101 cttgaacaac aaatgaatcg tttatttgta aatacaaatg attaccaagc tagtacattg
     5161 gcagtggaac atttgattca ttatggtcat gagaaaatag cgtttctttc ttttgactac
     5221 attgtaggtg aaccaagtgt tattcaagaa cgacatcaag cttatatcga tacattaaag
     5281 aaatatcatt taacctttaa atcgcattgg atatataaca actctgaatt tgagcgaatt
     5341 tatcaagaat tagctgaatt tactgcaatt gttgttactg gagattattt agcggctaaa
     5401 ttagtaaaat ttttaaaaaa taagaataaa cgagtgatgg aacaattatc gattattgga
     5461 tttgatgata ttacttttgc agaattaatg gatcctgcgt tgacaacaat acgactaaat
     5521 ccaggtaaca agggggcgat tgcttttcac caactgattg gactggccaa acaagaagaa
     5581 ataaaaaatc aagtactact attagaggga gaattgattt ctcgagactc tgttcacaaa
     5641 atcaaataaa aaagctctgt gaaataagta tagttaacaa taaaatatcc gaacgataga
     5701 gagaatgaac tgtgatctag cagttcctcg tcgcaaattc actagaatta gcattattta
     5761 ctctaaattg ttcggatatt ttttcttgtt tttaattatg attacagtgc ctatttttaa
     5821 cgaaatttgc ttattttcaa aatgtatgat tatatgttag taaacacttt caaatatgaa
     5881 aagaggagct attatggtag gaattaggga tgttgcaaaa cgggcgggtg tgtccattgc
     5941 aacagtatcg cgcgtgctaa atgaggatcc gacactttca gtaaccgatg aaaccaaacg
     6001 aaagattgca gaatcggtta atttttatca ttatgtcaaa aaaagtcccg ttgcaaaaaa
     6061 tttaacaaat attgcattaa taacgacagt ttctgaagta gatgagttgg aagatcccta
     6121 ttttcgaacg attcgcagag ggattcaagt cgaagcagaa caaagcaaag tcaatttgaa
     6181 gaaaattatt cgtttgtcag aaacaccgtt ggagcaagac gatttgctaa aatgtcaagg
     6241 tgtgttgatt attgggcagg tgctatcttc tgtgattgaa gaagtatcag caattaattc
     6301 gaatgtcgta gtgattgatg accctagtat tgaagctgaa gtagatgcag tttatactga
     6361 tttaacaaaa gcaacgaatc agcatttaga tcgattgtat aaaaaaggac accgaaacat
     6421 tgcgtttatc ggaggaggac gtgttcgttt agatagccaa ggaaataaac acgtgtcaga
     6481 tgatgattat cgaaaaatag cctatgagac gtggatgaaa caaaaaggat tagaaaaata
     6541 tagtcaaatt tatttagaag gctggaccac acttgacgga atgcaggcag cagatttact
     6601 tttgaaaaaa catgctaaag ataagttgcc gactgctatc atggtaggaa atgatcctat
     6661 tgctgtgggg gtttatcgtt cacttcaaaa aaatggtcta catattccag aagatatttc
     6721 gattgtgagt tttgataata ttgaagttgc tgaattttta acacctgcat taaatacggt
     6781 gaatgtgaat acagaagaaa ttggaaaact agcagttcgc atggttaatg aacgaataaa
     6841 agaagttaga aatgtttcga tcaaagtgat tgtttctaat aatattattg ttagagaaag
     6901 tgaaaaaaat atcgattaaa aaagtaaaca tccaaaaaag ggtgtttact tttttgttta
     6961 ctaatatttt tagtaaaaaa tctcgacaaa acattaagta aacagttatg gtgttgttaa
     7021 caaaaagaaa acgcattcaa aacaggagga attaagatga aacatttcaa aaaaatagct
     7081 ttaacaggga tagctgtttt aactgggaca ttactcgcag catgtggtgg gggagatagt
     7141 aattctaaag acgggcaagt aactttaagc tatgcgagtt gagatactga ccaagctcca
     7201 ggactaagaa aaatgttaga tgaatttgaa aaacaaaatc ctgatatcaa agtagaaatg
     7261 gagacaacgc cttgggatca atattgggtt aaattagaag ctgcaacaac gggaggtaat
     7321 atgccagatg tagtaaccat gcactctagc gaatcctata aatatatgtc acagaatgct
     7381 ttgatgggac ttgatgatgt tatcaaagaa aatgatattg acatgggaaa ctttactgaa
     7441 ggaattgctg atttctatac gcttgaagac gaactatacg caatccctaa agatgcttct
     7501 gtagtggggc tatggtacaa caaagaatta tttgataatg caggtgtcga ttatccagat
     7561 gaaacatgga catgggaaac actcagacaa gctgcaattg atttaacaga tgaatcaaaa
     7621 ggcatctatg gttttgcatc acatattgga acagagactg gctactggcc atttatttat
     7681 caaaatgggg gagaagtatt tgccgatgac aatacaaaat caggtttgaa taccccagaa
     7741 gcacaagaag ctttagcttt ttatcgagat attgcacttg ttgatcatgc ttcaccaaca
     7801 attacagaat tacaagatcc tgataagtta agtcgttttc aatcaggtag attagcaatg
     7861 atgattgaag gaaactggtt tgcaccggga tttttgaaaa atgactatct agttgaaaat
     7921 gttgcttctg ccgtgttacc taagggaaaa gttaatgcaa caattacaaa tggacttggt
     7981 tgggctgcca gtgcgagtac aaaataccca gaagaagtga aaaaactaat ggcattttta
     8041 gctagtgagg acgccaataa aatccaagcg gaaacggggg catcaatccc agcactaaaa
     8101 ggctttgaag acgagtgggc tgcttcttca gatacaattg atttgagtgc ttttggaaaa
     8161 atgttggagt atggagtccc tcgtccatac aataaagatg gattacgagc tgaacaagca
     8221 gaagttcaaa ttattaaccg tattctgtct ggtgaagtaa ctgttgaaga tggaaccgct
     8281 caaattgaaa aagaagtcaa tgcgatattg agcggaaatt aaattagtta attatacaat
     8341 atacaggcga aaggattcta tttcgtctgt atattaatgg atgaatacca aaaaggggga
     8401 tattatgaaa aatcgaattt ttggaaaata tcgtaaagat caagtgatat ggggctatat
     8461 ttttattttt ccaacggcat taggattaat tttattaaat atatggccag caattcaaac
     8521 agcaatttta agttttcaaa aaacggtagg gttcggtgat actgaatggg tcggattaag
     8581 aaattaccaa aaaatgtttg cagacggtga agtttttcag tctttattaa acacattaat
     8641 ttatgcagtg gtttcgatac ctagtattgt tgtattatcc ttgttggtgg cagttttaat
     8701 gaatagaaaa gttaaaggat tgtcgattta tcggacaatt tattttctac cagtcgttgc
     8761 tgcaccatcc gcagtcgcaa tgatttggcg ctggatgttc aataatgatt atggactgat
     8821 taataacatg ttggctaaaa taggtctcga tggaccagca tggttaacgg atccatcgat
     8881 tgcgatttat tctattatca ttgtaggagt ttggagtgct attggttata acatggtgtt
     8941 attactagcc ggcttataag aaattcctaa agattattat gaagcagcgg aaattgatgg
     9001 tgctagtcca atccagcagt ttttcaacat tacgttacca ttagttacac caacattatt
     9061 ttttgtagtt gtaacaacag ttatcaatgc tttccaagta tttgatgtta tttttatgat
     9121 gattactcct aatagtaccg ccatgtttaa atcacaatca ttagcctatt tattctataa
     9181 acattcattt attttaaatg acaagggata tggttcggca atcgttatgt tccttttagt
     9241 gattatatta attattactg caattcaaat gaagttgcag aaaaagtggg taaactatta
     9301 aggaggctat caaattatga aaaataaatt tttttcaaat gcgccaatcc atatcatgct
     9361 tattttaggt ggtattgcaa tggcattacc ttttatttgg atgattttaa cttcagggaa
     9421 aacgttggtt gaatctaccc aaattccacc tacaattttc cctgagaaat ttcagctaag
     9481 taactatcaa caagtacttg atttattacc attcatggct ttttatatca atacattttt
     9541 aatggttgtt ggacgagtga ttggctcggt tttattttca gcaatggctg catatgcttg
     9601 tgcgcgtcta agttttccag ggaaaaatat ccttttcagc ttagtactta ttcaaatgat
     9661 gattcccggt tccattttta ttactccaca gtatttatta gcacaaaaat tgggtatttt
     9721 aaatacaatt ccagcattaa tattaccagg ggtagttagt gcgtttggta cgtttttatt
     9781 aagacaattt ttcttaggtt tacctgatga attggaggaa gcggcctatt tagaaggtgc
     9841 caatatttgg acgatctttt tcaaaattat gttgccgctt gctaaatcag gattggttgc
     9901 acttggaata ttcacagcgt tgtttgcatt taaagattta atgtggccat tgattgtcaa
     9961 tacttcaatt gataagatgc ctttagctcc gggattagca aacttacaag gacaatatgc
    10021 cacgaacttc ccacaattaa tggcaggttc gttattagca atttggccaa tagttgtgat
    10081 attcatcgtt ttccaaagaa aattcattga aggaattgct acaacaggta gtaaattata
    10141 aaactattaa ttagtgattg gagagtaagt acatgacaaa aattacattt attggtgcag
    10201 gaagtactat ttttgcaaaa aacgtattag gcgatgcgat gttaactcca agtctacaag
    10261 atgcacatat tgcattgtac gatattgatg aaaaacgact aaaagaatca aatttaatgc
    10321 ttaaaaatat taataaaaat tcgaatgaaa atcgtgctga tattgtttct tattatgatc
    10381 gcaaagaagc gttaagagag gctgattttg ttatcaatgc tattcaagtg ggtggttata
    10441 aaccttcaac agtgattgat tttgatattc ctaaaaaata tgggttgcga caaactattg
    10501 gtgacaccat ggggattggt gggattttta gaaccctacg gacactacca gtaatgttag
    10561 actttgctaa agaaatggaa gaagtttgtc caaatgcttg gttattgaac tatacaaatc
    10621 cgatggcgat gttaaccatg ggtgttttaa aagcgacgag tattaagact gttggtttat
    10681 gtcatagcgt tcaagtatgc gtaccagaat tgtttgagca tttaggaata gccgatcaat
    10741 atgacttaaa tgactttcaa tggaaaattg caggcattaa tcatatggca ttcttgttgg
    10801 aaattacaaa aaatggggaa gatttctatc caacaattcg tcagttagct gcagaaaaac
    10861 caaatccaca tccagattct gtacgttttg aattgttgaa acgttttggt tactatatta
    10921 cagaatcaag tgaacacaat gcagaatacc atccatactt tattaaatca aaatacccag
    10981 aattgattga cgaattaggg attccgttgg atgaatactt acgtcgttgt gaacaacaaa
    11041 ttaatgattg ggaaagcatg cgagaggaca ttgtaaataa tggggagtta acacatacga
    11101 gaagccgtga atatgcttca tatatcatgg attctattac gacaggaact ccgtcagtga
    11161 ttgcaggaaa tgtcttaaat aaaggcttaa tcacaaactt accgacagat gtttgtgtgg
    11221 aagtaccttg tttagtcgat aaaaatggcg tgcaaccaac ctatgtcggt gaattgccaa
    11281 cacaattagc tgctttaaat cggacgaata tcaatgttca agaactgaca gtagaagccg
    11341 ctttaacact agataaatca aaaatttatc aagcagcgta tttagatcca catctatcat
    11401 ctgaattgtc tttaacagat atcaaaaatt tagtggatga tttaatcgaa gcacatgggg
    11461 actatttacc tgaatataat taaaaagaag ctgaaagagt aaggaatttt caattcctta
    11521 ctcttttctt agttaaattt agtaaaaata ttgacaaaac acttagtaaa cagttacatt
    11581 agtgttaaca aaaagaaaac gcattcaaat tgtataagga ggagcataaa atgaagtggt
    11641 ctaaaaaaat tattttagct tgtatagcac taggagcaac gatctctttg actgcttgtg
    11701 gaaaaaatca ggaagatgca agcggtaagg tagaaataga gttttttaat caaaaaaaag
    11761 aaatgactca aacgattcag gagattgcta aagattttga agcaaaaaac cctgatattc
    11821 atgtaaaagt tgtggatgta ccgaatgctg gtgaagtaat taaaacacgt atgcttgcag
    11881 gcgatgtacc agatgtcatt aatctttatc ctcaaagtat agaattgaaa gaatgggcga
    11941 aagctggtta tttagaggac ttaaccgaag aaccgtattt agagaatatc aaaaatggat
    12001 atgcacaacg ttttgctatt gaggatcgtg tatatagcat accattaacc gctaatgtct
    12061 atggttttta ctataacaaa actgcttttg aggaaatggg gattcaagca ccagaaactt
    12121 gggcagaatt tgaaaaaata gtagctgaga ttaaagatca aaacaaagta ccgtttgcga
    12181 ttgcaggagc agaaggctgg actttaaatg gttatcacca actagctata gcgactgctg
    12241 ctggtggtga agaggaagcg aacaatgttt ggcgtttctc agaagttaat ggaattaatg
    12301 ctgaaagcaa agaaatgcaa aaagatttta atcgattaga tttgttaaga gaaccaaaag
    12361 ctttgcaaaa caactggcaa ggtgccggtt ataacgatac agtagttact tttacaaaag
    12421 gtgatgcgtt gattatgccg aatggttcat gggcaatgcc gatgattcat tcacaaaatc
    12481 cagattttga agtaggaact tttccttttc cagcagatga agcaggtcaa agtctaacga
    12541 ttggcgcagg agacctagcc ttatcagttt ctgcaacttc tcaacataaa gaagaagcca
    12601 aacgttttgt agaatatatg acaacacctg aagcgatgca gaaatattat gatgtagatg
    12661 gtgcaccgtg tgctgttgag ggcgttatcg agaatactca ggattcacca ttaagtggtt
    12721 taactgaact tgcttttact gatcgtcatt tagtttggtt agccaaagat tggaatagtg
    12781 aaaatgattt ttatacctta acaacaaatt atttacacaa tggcaatcaa aaagccatga
    12841 ttaacgcatt aaatgctttc tttaatccaa tgaaagctga cggaaggtag gaaaataaaa
    12901 tgaaaataaa agaaatttta aatcgttact gggcaatcat ttttgtagca attccaatta
    12961 ttttgcagat gatttttttc tactttccaa tggttcaagg tgctttttac agtttaacta
    13021 attggactgg tctgacttat aattttgatt ttgtgggttt gaacaattat aagattcttt
    13081 tttctgatcc gaagtttatc aaatcgattg gcttcacttt aattgtgacg ctttgcatga
    13141 ttgctggaca aattggtttt ggtattttga ttgcgcgggc gttgaacgca aatattaaag
    13201 ggaaaacatt ctttagagca tggttctttt ttccagcggt cttatcgggc gtggttgttt
    13261 ctttagtatt taaacaaatt tttaactatg gtttaccaaa aattggtagt atgttaggca
    13321 ttgaattttt acaaaccagt cttttaggaa caacatgggg agcgattttt gcagtaattt
    13381 ttgttttact ttggcaagga attgccatgc cagtcatcat tttcttagcg ggttttcaaa
    13441 gtattccaca agaaattatt gaagctgcta gaatggatgg ggcgaatgat aagcaaattt
    13501 tttggaatat tgaaatcacc tacttattac caagtatttc aatggtattt attatggcat
    13561 taaaaggtgg cttaacagca tttgatcaaa tttttgcttt aactggtggt ggaccaagcg
    13621 atgccacaac atctttgggg ttacttgttt ataattatgc ttttaaaaac aatcagtttg
    13681 gttatgcgaa tgctattgct ttagtcttat ttatcttaat tgggattgtt tcactggcac
    13741 aattaaaatt atcgaaaaaa tttgaaatct aggaggtttc ccaatgaaaa catcaaaaga
    13801 aaaaaatatt tctaaatact tgcttttatt attagggggc ttcatgattt tagttccctt
    13861 agtttcaacg ctattcggtt catttaaaac aacacccgat attatgaata atttctttcg
    13921 ttttcctaat ccagcgactt tggaaaattt ccgaagattg ttagcggatg gaattggtca
    13981 ttacttttgg aattcggcaa tcatcacaat cattgcagta gttgctgtga cagcaattat
    14041 tcctatggct gcttattcac tagcaagaaa aatgactcgt gggaaagcat acggattgat
    14101 gtatttattt ttaattttag gaatttttgt tcctttccaa gtaattatga ttccgattac
    14161 cgttatgatg agtaaaattg gtttagcaaa tatgtgggga ttaatcttac tctatctcac
    14221 ttatgccgta ccacagacac tatttttata tgttggtttt attaaactaa atgtgcctat
    14281 cagtctggat gaggctgctg aaattgatgg ggctagtcac tttacaactt atttcaaaat
    14341 tgttttccca atgttaaaac caatgcatgc gacgactatt attcttaatg ctctatggtt
    14401 ttggaatgat ttcatgctac cattattaat tttaaatcgt gattctaata tgtggacact
    14461 accattgttt caatataatt atacgggaca atattttaac gactatggtc caagttttgc
    14521 ttcctataca gtaggaattg tcacgattac actagtttat cttgctttcc aaagaagtat
    14581 tatagctgga atgagcaatg gagcagtgaa ataaccaata aattattctc attataaagg
    14641 agttcataaa cttatgatta tatttgacaa tcacacacaa acatttcatt tacaaaataa
    14701 aaaaattagc tatctattag gaatcgaaga aaataattat ttaacgcatc tttattttgg
    14761 aaaaaagatt acgcactatt ctggtgggta tcgctacccc cgaacagatc gttccttctc
    14821 acccaacaat gcaaatgcgc aagatcgttt attatcttta gacacattac cattagaatt
    14881 tccaggatat ggacatggtg attttagaga accggcgcat aatattaaat taggaaacgg
    14941 tagtcgtgta aatgattttc gttatgatag ctacgaaatt caaaaaggaa agcccagctt
    15001 agcgggattg ccagccttat atacggaaga tgatgatgaa gctgaaacgc tgattattca
    15061 attgaaagac gcgattagtg gattgacttt acagttgttc tacacaattt atcaaaatca
    15121 cagtgtaatt attcgttcta cgcaattagt aaataatagt gatgaaacag tggagattaa
    15181 ccgcttagct agtcaatcaa ttgattttcc aaatcgtcca ctcgagttaa ttcatttaaa
    15241 tggtacctgg gcaagagaac gtcaaatgac tagagaaaaa attcatacag ggataaaagt
    15301 attagatagt aaacgcggat caagcagcca tcaacaaaat ccgtttgttg ctttagtaga
    15361 tgccaaaaca gatgaatttc aaggagaggc atatggattt aatttagttt atagcggtaa
    15421 tcacgaaacg gtgattcaaa aagatccatt tgaccaaaca cgggtcatta tgggaattaa
    15481 ttcatttaat tttggttggc aactaaaaac tggcgactca tttcaaacgc cagaagtcgt
    15541 gatggtttat agtaatgaag gtctgaatac gttatcacag acgtatcatg aattgtataa
    15601 tcaccgttta attcgtggaa actatcgttt aaaagaacgt cccacgttaa tcaacaactg
    15661 ggaagcaacg tattttgatt ttaacgaaga aaaaatcatg aatattgtcg atgatgcgaa
    15721 gaacttgggc attgaaatgt ttgttttaga tgatggttgg tttggcggtc gtgaaaatga
    15781 cttcacttca ttaggtgatt gggttgaaac agaaggcaaa cttgcttgtg gtttggaaaa
    15841 tttagccaag caagtgcatg ataacgggat gaaatttggg atttggtttg aaccggaaat
    15901 gatttctgaa gatagtgaac tatttcgagc acatccagat tgggcattgg gtattccagg
    15961 tcgtgaccgc tcaattagtc gaaatcaata tgttcttgat ttttcaagac aagatgttcg
    16021 tgataatatc tatcaacaaa tgacggcgat ttttgatcgt gtaccgattg attatgtgaa
    16081 gtgggatatg aatcgaaata tgacagaagt ctattctgtc ttgttggact caacgatgca
    16141 aggtgaagtg gctcatcgtt atatgttagg gttatatgaa tttttagaaa agttaacaac
    16201 gaattatcca actattttat ttgaaagttg ttcgggtgga ggcggtcgtt ttgatgcagg
    16261 tttcttgtat tacatgccac aaacttggac gagcgacaat accgatgccg tggctcgtct
    16321 gaaaattcaa tatggaacca gcttagtgta tccaatttca agtatgggtg cgcatgtttc
    16381 agcagtccca aatcatcaaa caggaagaga aacgagttta actattcgtg gcaatgctgc
    16441 gatggctggt gtttttggtt atgagctaga tctaacgcaa ttaacttctg aagaaaaagc
    16501 agaaattaaa gaacaagttg ccttttataa aaagcatcgc caacttttgc aatatggaac
    16561 tttttatcgc ttggaaagtc cgtttgaagg caatgatact gcatggatgt ttgtctcaaa
    16621 agaccaaaca gaagccatgg tcttttattt ccgagtatta gcagaagctg cgcatccatt
    16681 agtcacattg aaattagcag gattagcaga agaagcaact tatcaaatgg aagatgtaac
    16741 atttgctggc gatgaattga tgaatctcgg tttttatatt gactcagaac tacatggtga
    16801 ttatgcgaca caacgctttt atttgaaaca gatatagtag cttgaattta caatataagt
    16861 gcaacaccaa aagagactca ttccaaacga agaaaatatg ttttatttac acgcatttac
    16921 taaaaagcag cctgatttga attgggaaaa tgaagaagta cgtgaagaaa tctatgcaat
    16981 gattaactat tggttagata aaggactagg cggatttaga atcgatgcga tattaaacat
    17041 caaaaaaaaa ctagagtatg gcatctttga atctgatgga gaagatgggt tagccttcat
    17101 tgggccctgg attcttaatc agtcgggcat tgaagtgtgg ttaaaagaaa tgagagagcg
    17161 gacatttaaa ccacataata gtatgacggt agcagaagcc gatgttccta atgaacgttt
    17221 agatgaatat attggtgaag atggctatta ttcgatggtt tttgatttta gttatacgga
    17281 tattgatgta cctgaaactg gtgaatggtt taaagatagc caatggacat ggacggatat
    17341 gcgtaccaat atttttacga atcaattagt tacgcaagat aaaggttggg gcgccttata
    17401 tttagaaaac cacgatcaac cacgttcaat taataaatat attccagaag aatggatcaa
    17461 tgattatagt aaaaaaatgt tggcgacgct ttttatgtta ctaagaggta ccccttttat
    17521 ttatcaagga caagaactag gaatgaccaa catagaaatg gcatcattag aagattttga
    17581 tgatgtagct acgcattctc agtataaacg ggcattagaa tatggattgg caccagagca
    17641 agcattgaaa gctgtcaata aacgaagtcg ggataattct cgcacaccta tgcaatggaa
    17701 tacgcaaaaa aatgccagat tctcaacttc tgataatgta tggttaaaag taaatcctaa
    17761 ttatccagaa ctcaatgcag aagctcagtt aaccaatcaa cagtcggtat tcaattatta
    17821 tcatcaatta attaaattaa gaaaaaatag tgtatacaaa gacgtgctaa tctacggaca
    17881 atttctacca gtgaaagata aaaatgaaca tattttgctt tatgaacgcc aattagaaga
    17941 taaaatcgta ttagttatct taaacgttac agcaacagaa caagaatatt atgtagatga
    18001 acagtaccaa caagtattaa tcaataatta tgaagatgtt ttattagatc aaagtcgaaa
    18061 attgcgttta cgaccgtttg aaagtattgt attagctaat tatggaggac aattatgaaa
    18121 attaaaaacg aagcaatgct catcacctat gcagatagtt taggaaaaaa catgggcgaa
    18181 ttaaaacaag tcttagacaa acatttacaa ggtgttattg gtggtgtaca tctgttgcct
    18241 ttttttccat cgacaggtga tcgaggcttt gcgccgagtg attatacgac agttgatcca
    18301 gctcttggaa catgggaaga tgtggaagca ttaggtgaag attattattt gatgtttgat
    18361 tttatgatta atcacatttc tcgtgaatca aaatttttcc aagatttcaa aaaaaaccat
    18421 gagcagtcac catacaaaga aatgtttatc cgtattcatg aatttttccc agaaaatcga
    18481 ccaacacaag aagatattga tttaatctac aagcgtaaag acaaagcgcc gtttcaaacg
    18541 gtagaatttg cggatggtac gacagaagag gtttggaata cctttgggga agaacaaatt
    18601 gatttagatg tgaccaaaga agttgtgaaa gaatttattc gagaaaccat caaagatatg
    18661 gcgaaccatg gctgttcttt aattcgatta gatgcgtttg cttatgcgat taaaaaatta
    18721 gataccaatg atttcttcgt ggaaccagat atttgggaac ttttagacga agtgcgtcaa
    18781 gaagcagcga aatatgacgt cgaattatta ccagaaatcc atgagcatta ttccattcaa
    18841 atgaaaattg ccaatcatga ttattatatc tatgacttcg ccttgccaat ggtcactctg
    18901 tattcacttt atagtggaaa atcagaacgt ttagccaatt ggttgaaaat gagtccgatg
    18961 aagcagttta caacattgga tacgcatgat ggaattggtg ttgtggacgc tcgtgattta
    19021 ttaacagatg aagaattgga ttacacctct gaggaattgt ataaagtcgg ggcgaatgtg
    19081 aagaaggtct attcaagtgc caattacaat aatttagata tttatcaaat taacagcacc
    19141 tattatagtg cattaggcga taatgaccgt agctatctgt tagcacgtgc gattcaagta
    19201 tttgcgccag ggattccaca aatttattac gttgggttat tagcaggaaa aaatgatatt
    19261 gaactgttgg aagcaacgaa agaagggcgt aatattaatc gtcattacta tgatttagcc
    19321 gaaattgaag aagaagtgca acggccagtg attcaacaat tatttgcact tttaaaattt
    19381 agaaatgaat ctgcggcgtt tgatttggat ggaacgattg aagtagcaac gccatcagaa
    19441 tcagaaatag tgattacacg taaaaataaa aacggtagtg atagtgcaac attaacggct
    19501 aacttggata cgaaagaatt tatcattgag tctaaagata taaaaatttt cactagtgtt
    19561 gcattaaaat tttaacctat tccatagaac aaaaaatcct ttatactagc acttggatga
    19621 cttatccaag accaataaaa aggactcata catggataag tataaaacaa atacagcatt
    19681 taataaatgg ttttctgcca taaaattagg aaaactttct gtcaactccc aaaaatgtat
    19741 ttttgattac aattcgtatc gtaaaaaact gtcttttgaa gctgtgctca agctctttct
    19801 ctacgcagtt aatgaagaaa aagaaagttt gcgtgatttg agtacagctc ttatcgaccg
    19861 atcgctccaa ctagaaaccg atgtgtcatc gattagtcat acccaattgt ctcgagcatt
    19921 taatgcactt gaacctaaag tgttagaaga aatttttcaa caactgttag aaaaagttca
    19981 acatcaaatc aaaccaacca agcgaaatca tctgtatctg attgattcca gtacgttttc
    20041 gcttagttta aaacgtcata agtgggcgac tttccgtcaa acaaaatcag gtgtcaagtt
    20101 acacttaaac tattgttata tggacgatac tacgatgtat ccaactgatt ttaccttaac
    20161 aaatgcccat gaacatgatc tcaatcaact accagtattg gtgaatcaac cagaggcaac
    20221 ttacgtgttt gatcgtgggt attaggactt tgaaaagatg gatcagatgc attgggatgg
    20281 ctatttcttc gtcactcgga ttaaaaaaaa caccaaagtt cacgtgatag atactctgga
    20341 aacatcgcca gaaacagaaa ttctacgaga cgagctggtt cgtttaggat ctaaaaccta
    20401 tctaaccgcc aatttccgac tagtcactgt tcaagataag aacggacgtg tgtttcaatt
    20461 tatcaccaat cgaatggacg tctcttcaaa agaaatctct gatatgtatc acgctcgttg
    20521 gcaaatcgaa ttgtttttca agcacatcaa acaacacatg accattaaaa ccttcttttc
    20581 tcaaagtgaa aaaggcgtgc agaatcaatt gattttaacg atgatttccg ctctcctcac
    20641 gtttctcatc aaattggaga cgaaaacgga aaaatcagtg ttccaaatca aacgattctt
    20701 tcgttattta ctgtttcagc cctttgaatg ttgtctagaa aaattaattc ccacatgaga
    20761 aaaaagaaat ttggctcgtt acttttggtt ttcagttgaa taacagataa aaaattttct
    20821 ggaaaaagtc atcctctgcg tcagcacagt tttttttgac ttattttcag aggaaatagt
    20881 tttcagaata ttctaaaatg ttttatgcaa cactagtgat attttttaga attagatttc
    20941 taatgtgcat tttttgagta ttaaggagca agtatacaaa gatttataaa gctgattaaa
    21001 tttgaataaa aatatgcagg aacgctacta tttttagtag tgttcttttt tatatttgaa
    21061 taaaaaatgt ggtggttaaa tggtggttat aacaaaaaat gaattaatga aattaggtta
    21121 tgggaagtat cagtctgaga gaatcatacg acaggcaaaa gcaattatgg tttcaaaaag
    21181 ctatacctat tacgaaaata gaagattagg actagtacct gttgaatctg tagaagaagt
    21241 tttaggtatg aagatagagg tagaggagga ttttcagaat catggttaat tttaaaaaag
    21301 ataaaaatgg gaaatatcat tttactgcta gtttgggatt cgacaaacga acagggaaaa
    21361 gagtacaaaa aaacgtagag gggttaaaac agttaaagaa gaaaaaaagc gtataccgaa
    21421 cttgtttcta attatggaaa agaagcagaa acttctcaaa gtactatgtt gtttgaggag
    21481 tttttttata aattattcct accttattat caagggaaag tagaagaaag aacatataaa
    21541 aatcggttat ctacaatcaa aaaaactttt cttatttctt taaaatggag ttaagagaaa
    21601 tacgtccaat tgacattcaa aagtggcaaa ataatttatt gagcaaatat aaaaattctt
    21661 atgtaagaaa catgtatggg atttttcaga tgtcattaga tcgagcagtt gttttgggac
    21721 tacttgaaaa aaatcctgca aaagttattg gaaatgtgaa gaaaaggaaa actacagttg
    21781 atttttggac aaaagaagaa tttacaaaag ttatttcaac tttttatatt gatgatttgt
    21841 accaacattt tggttttatt tgtatgtggt tactttttat gaccgttatg cgtttgggag
    21901 aagccactgc attaacttgg aaagatattg attttgaaaa taaaaacttg cgtgtgaata
    21961 aaacactcta ttataagaga gccaaaacct atgagtttgt agaacccgaa acggcttcta
    22021 gtattcgtac aatcgaacta gacgacaata caattacctt tttaaaagct tggaaggagc
    22081 gtcaagtttc aataggaggc atgtcgtttg tactttctta taatggaaca cctactcaga
    22141 aatatacaat ccgtcatatt atgagaagac atgcaacgat cgttggtgtt catataatcc
    22201 gcatacacgg attaaggcat agtcatgctt ctttgcttat atcacaataa aaagatgcac
    22261 ttcgaattaa agaaaggtta aaacatgctg atgtacaaac gactttagga acttatggtc
    22321 atttgtatct aaattcattg agagagataa caaatgatcc cacagattat atccaaacta
    22381 atttttctaa aatcaaaccg acacctattt acgataataa aaaataaatg ataaacaaaa
    22441 gtaggatcac accaaaagaa acacactaga aaaactataa tacaggcttt tctagcgtgt
    22501 tgtttcttat ttccgcctat ttatacatta tactcataaa gcatttaatg catcgatcgt
    22561 agccgcattg tctatatggg tatataaagt tgttgtattg gtacttgaat gtcccaattg
    22621 ttgggcagtt aataccaatg aatttgtctg ttggtagagt ctagtagcta atgtatgacg
    22681 cagtttgtga gggctgactt gaattctaaa gccttttgaa tattttgcta ccatccgttc
    22741 gattgcaata ccggatatac gcttggcttc tccttggtaa attgctagaa ataaagcttt
    22801 ttcgttttct gttactttat atcgttcttt ccgaatagct agataattag ctaaatattc
    22861 cattgcgaat gctgctatat tgacagcatc tcttttattt ccttttctag ttactaccac
    22921 attattctta tctaaagata ggtcatctaa attaatattt ataacctcgg agagtctcaa
    22981 tccagatcct aaaatcagtg cattgatcgc caggtctctt tctacatctc gttgataata
    23041 gatcaaagct cgtttactca aagtattttt atattcattg gcaatataat ctagataatt
    23101 ttgtgtatcc ttatctagaa ataatttttt ctcaatggca ttggcacgtg cagctaacgt
    23161 agttttatct gtgatggttg ctacttgatc cataggatta ttgtctaagt atggttttcc
    23221 tagaggatta ttggaacttt tcgaaagata tttaaataga acctttaatg ccgtgatatt
    23281 tctattgata gtagaagtag atagacctaa gttattatta tctttgagct tagtttttgt
    23341 tgtctctttt tttacatttt cttttgtgcg acgtttcaaa tgagatttgt acaagtttag
    23401 gtcataggca gttaactgtc ttatcgaggt gatagaagta tcaggaacga tagactcact
    23461 cagaagccat tcataaaaga gcttataatt ttttagatat tctaaagtgg ttcgaggtga
    23521 ttgatcttta tcctcacgat cttgaacaaa atcttttata taatcgggca tatgaagtat
    23581 gattgtatct attttttgat atatttctgt atttttcatg tcattcatcc tttcaataag
    23641 taggggagaa gagtatctct ataattggac tataccaatt atagaatgtt ttgtcaattg
    23701 gtatgctcta aaatttatta atttgtttca aaaaaagcat ataaacaggt atacgtttaa
    23761 taatttaaaa taaatggtat acataaatag ggaaggaaat agaagggagc aatctaaatt
    23821 ttttgaattt tcttaattaa ttatatagaa agaacaatct ttctatagag ttagacatga
    23881 gagggactat gcttgattta aaatcaacaa gcgtatatac tgtatataca aagaaaaagg
    23941 agggagaaga ctatggcaat taaagaaaga aaatttaggg aagtggggaa ctcagtagta
    24001 ttaactattt caaaggagtt tcttaaagaa aatgggttaa atcccggaga tacggttttt
    24061 ttagatgaag aaaaacttcg agcggcaatt acaaaaaaac aagccttatc tgatgaaatt
    24121 gatttagcaa tatctcaagc attaactgaa tatgatacta cttttagaga gcttgtcgat
    24181 aaatgagtat tcattaccta gaagccaaag atattataaa aatgaatgta atccaaataa
    24241 aaaaatactc tcctaatgaa ccccttggtg taaaggatgc gaatgcttta gaaatgtgtg
    24301 tagggcaact tcgagcctca gcttttggtg aagaggttta tccttcgatt tatgaaaaag
    24361 cagctatcct acttatacaa ttgataaaga aacatccttt tcacaatgca aataaacgta
    24421 ctgctttctt agctactttt gtctttttga aaatcaatgg acagctttta gctattgatc
    24481 aaaaagatgc tgtaaattta gcggtatata ttgctacgta tgataaggat ttcgatcaac
    24541 taaaatatga agtgatcaac actataaaga cacatacgca gcccatttaa ggagttactg
    24601 tatgtgtctt tatagggtta ttagctgcta tagcgaaata attgaagtaa attacaaata
    24661 gctataattc aaatccaact atatagaaaa cgtaaatttt ctatatagtt ggattaagat
    24721 aacgactatt tattttatca tatttttact ataccctata cttttacttg gtatatttat
    24781 gaataatcgc aaatttaccc ctatactact agtttgtaag ttagattaat aaaaatacta
    24841 aagttatttg ttcttttatt aaatacacct acaacttata aattgatggt atttagataa
    24901 cacgaaagaa taaaaataac agctgatagt aaagtttgtt gacgattgct actcaatcga
    24961 aagtgagtta gtgcgtatga acttgcttct gaaaacttat gaaatgaaag accttttaca
    25021 tcttgcagaa atcggtactg ggattaaact aagcaataaa aactaatgtt cccctcattc
    25081 tttgaacttc tactatttga tttatttatt ctgataaatg acgatttcac cttcaggatc
    25141 ttcaaatccc gtatagaatt tatttaagat ctcttcagta tcagtaatag gataggcgat
    25201 aggccacata ccctttgatg gttagaataa actgctcaaa atctgtatcg tatgttggct
    25261 ttagcatatt aacaaaggct atctccaaat tataaaccat aagcgttgaa tcgttcttaa
    25321 caatttcaat tcttcctaac ccaaagtcag aagatataat taccggactt gccttaccct
    25381 tcatgtgggt tgttgagatt ccatatttaa agggaattgt cggtactatc ggcggtctga
    25441 aaaacatccc aaaaataaaa aaatctttcc cctgctagtc tcctaagtcc ctttgtagcg
    25501 gatatggcac cataatctca ttttatcgaa gtgagtcatg gtttatctgt tggccaacca
    25561 aaattgatac aatctaacga tgcaccctct acggcattct aggggagtgc acttgcattc
    25621 acccaataaa aaccggcagt tagcaaagaa cttatgtagt cgtatgttct tactgttaac
    25681 atacaagagg tctcctttcc acaattgctt agcagtaaac gggaatttac aaagctaatc
    25741 tcttccattt tcttatcttt gttctaaaag tcccaaatgg agcaactgta tttacatgaa
    25801 taaatttata tacttcccat acagccgttt tggttgcctc gtctgcccat tttcttttgt
    25861 gtgcagtgaa tagatcttca tcactcattg aatcaatcat catgttaata tcatcaatat
    25921 tacgattgag aatttcctct aattctttca atggtagatg tgcataggta tttgtaaacc
    25981 acttatataa ctctcctaac tgattccatt taaattgatc agaaggcgtt ttaacagata
    26041 atccatttct ttcatcattt tcccatttta gaattaaatt cgtccaacca acttgatatg
    26101 ctaaattttc agcaggtgtt ctatccactt cctcaactct tttatccttc aattcttcag
    26161 gaatagtttc aaattctaaa atatatttag tataagtcct atttatttct ttttttaatt
    26221 cttctttatt tttatatgtt ctcattatat tacctccata aattcctaat tgtctccttt
    26281 aattcatcta taatttcatc cacgatattt taatccgttc tacaatgcat atctgatatg
    26341 cattgactgt ctaactcata tgtgaatgat ttataaaata tcccaacgtc tcatctaaag
    26401 aaatgttgat cgtcagcaag gagctaaaaa atacgaatat atttcttttg tagtaaaatc
    26461 ggactggttt gcatatcatt ttcttcgtaa atatcaaaat catgttaact actatcaatt
    26521 actaataaaa actaccactt taagcgttaa gaactgttaa gtttagttaa gtgaggatag
    26581 ttcacaaaat ccattgttaa acttcacgaa agtggtaaaa ctcaaaatgc tttatatagg
    26641 attacagcat cgctctttct tcgattgcgc gttgggtaaa aaatattatg aagtaaaaat
    26701 tgacgatgac acgttcctta cggctcatca gatgaaacag ctacaaaaac gaaatgatca
    26761 actcgaagaa aatttaattc taaaaaagcg attgccatat tcacgcctcg ctcgacgaac
    26821 tattaaaggt caagatttac ctgaagatca tcctttttag gaaattgaag ctttttatgt
    26881 tgcacaatgc atttataata ctacaatgat gttttcacca gatattcttc tagggtgcgg
    26941 tgtaatgaaa cagatacact tgcaaaagaa aattcaccgc gaatttacta aaattatgaa
    27001 taattatgtt aatcttc
//
LOCUS       DAYYCY010000019        26764 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000019 DAYYCY010000000
VERSION     DAYYCY010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26764
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.019"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(114..494)
                     /gene="yajC"
                     /locus_tag="ACHAZB_000676"
     CDS             complement(114..494)
                     /gene="yajC"
                     /locus_tag="ACHAZB_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFM8613371.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(591..1736)
                     /gene="tgt"
                     /locus_tag="ACHAZB_000677"
     CDS             complement(591..1736)
                     /gene="tgt"
                     /locus_tag="ACHAZB_000677"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFM8613372.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            1954..2652
                     /locus_tag="ACHAZB_000678"
     CDS             1954..2652
                     /locus_tag="ACHAZB_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFM8613373.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(2820..5459)
                     /locus_tag="ACHAZB_000679"
     CDS             complement(2820..5459)
                     /locus_tag="ACHAZB_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFM8613374.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(6078..7109)
                     /gene="queA"
                     /locus_tag="ACHAZB_000680"
     CDS             complement(6078..7109)
                     /gene="queA"
                     /locus_tag="ACHAZB_000680"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFM8613375.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            7302..8477
                     /locus_tag="ACHAZB_000681"
     CDS             7302..8477
                     /locus_tag="ACHAZB_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM8613376.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            8481..9122
                     /locus_tag="ACHAZB_000682"
     CDS             8481..9122
                     /locus_tag="ACHAZB_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8613377.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            9119..10036
                     /locus_tag="ACHAZB_000683"
     CDS             9119..10036
                     /locus_tag="ACHAZB_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8613378.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            10033..10704
                     /locus_tag="ACHAZB_000684"
     CDS             10033..10704
                     /locus_tag="ACHAZB_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8613379.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(11245..11733)
                     /locus_tag="ACHAZB_000685"
     CDS             complement(11245..11733)
                     /locus_tag="ACHAZB_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HFM8613380.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(11934..12200)
                     /locus_tag="ACHAZB_000686"
     CDS             complement(11934..12200)
                     /locus_tag="ACHAZB_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFM8613381.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(12200..12463)
                     /locus_tag="ACHAZB_000687"
     CDS             complement(12200..12463)
                     /locus_tag="ACHAZB_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFM8613382.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            12984..13124
                     /locus_tag="ACHAZB_000688"
                     /pseudo
     CDS             12984..13124
                     /locus_tag="ACHAZB_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(13266..15089)
                     /locus_tag="ACHAZB_000689"
     CDS             complement(13266..15089)
                     /locus_tag="ACHAZB_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFM8613383.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(15687..18392)
                     /locus_tag="ACHAZB_000690"
     CDS             complement(15687..18392)
                     /locus_tag="ACHAZB_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HFM8613384.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(18698..19645)
                     /locus_tag="ACHAZB_000691"
     CDS             complement(18698..19645)
                     /locus_tag="ACHAZB_000691"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HFM8613385.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            19835..20104
                     /gene="rpsN"
                     /locus_tag="ACHAZB_000692"
     CDS             19835..20104
                     /gene="rpsN"
                     /locus_tag="ACHAZB_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFM8613386.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(20280..20642)
                     /locus_tag="ACHAZB_000693"
     CDS             complement(20280..20642)
                     /locus_tag="ACHAZB_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFM8613387.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(20653..21774)
                     /gene="alr"
                     /locus_tag="ACHAZB_000694"
     CDS             complement(20653..21774)
                     /gene="alr"
                     /locus_tag="ACHAZB_000694"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFM8613388.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(21790..22140)
                     /gene="acpS"
                     /locus_tag="ACHAZB_000695"
     CDS             complement(21790..22140)
                     /gene="acpS"
                     /locus_tag="ACHAZB_000695"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFM8613389.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(22270..23781)
                     /locus_tag="ACHAZB_000696"
     CDS             complement(22270..23781)
                     /locus_tag="ACHAZB_000696"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFM8613390.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(24103..25455)
                     /gene="murF"
                     /locus_tag="ACHAZB_000697"
     CDS             complement(24103..25455)
                     /gene="murF"
                     /locus_tag="ACHAZB_000697"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFM8613391.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(25608..26684)
                     /locus_tag="ACHAZB_000698"
     CDS             complement(25608..26684)
                     /locus_tag="ACHAZB_000698"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFM8613392.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
BASE COUNT         7417 a         5735 c         5000 g         8612 t
ORIGIN      
        1 cgattattca atattgagca atatatccca tgctcttatc tctttctttt ttgaataaaa
       61 aatgaggtag gacatctttc tgccctacct cgtctttaaa taagctttga tacttatttt
      121 tcttccttct cttctgtctc ttccagcgtt tctgttgttt cagtttttac ttcaactgtc
      181 tcagctggtt cttctgttgt ttcaacagta gtcgttgcat catttgttac gacagtccct
      241 ggtttgaccg ttttgattgc tgcacgatcg tactcaagga agatcccttc gcagtctaaa
      301 acaactgtcc gtttgtcatt gtcgatttct gaaacaactc catgtaatcc gccgatcgtt
      361 acgactgaat cacctggttt catgctatct aacaagtttt gacgttcttg ctgttgtttc
      421 ttttgcgaac gtgacataaa aaaccacatt gctaatagcg cgataaacat cacaagcatt
      481 ggtaatgttc ccattttttt cacctcacta attctgtatc tacaaaaaac actttagcag
      541 aaaaatgctg ataacactag ttttttactt aatttttaaa agattttcct ttagaagctt
      601 tttgcgtttt ctttattgaa cccgtattct tcaaaaaagg cttctctaaa ttcaagcaaa
      661 ttatcatcca tgattgcttg acgtacttgt ttcattagat tcaacaagaa gtataaattg
      721 tggtaagaag tcaaacgaat cccaaatgtt tcatcacatt tgatcaaatg gcggatatag
      781 gcacgtgtat agtttttaca tgtataacaa tcacatttct catcaattgg acggaaatca
      841 tgggcatatt tcgcattttt cactactaga cggccttgag aagtcataca tgtgccattt
      901 cgagcgattc gagtaggcaa gacacagtca aacatgtcta ctccacggat aacaccatcg
      961 atcaatgaat cagcagcacc gacacccatc aagtaacgtg gtttgttttc ggggatcaac
     1021 ggtgtagtga attctaatac acgattcatt tctgatttag gttccccgac agataacccg
     1081 ccgattgagt aaccagggaa atccatcccg actagatctt tcgcgctttg acgacgaaga
     1141 tcttcaaacc ctgctccttg gatgattcca aataatcctt gacggtctgg attagcatga
     1201 gctttcaatc ctcgttctgc ccatcgagac gtacgttcta ctgatttttt cacgtagtcg
     1261 taactttcat cgaatggtgg acattcgtca aaactcatca tgatatcaga gcccaattta
     1321 ttttggatgt tgattgcttt ttctggtgat aaaaacatac gcgaaccgtt caagtgattt
     1381 ttgaaatgca cgccttcttc ttcgatattc cgcatatcac ttaatgaaaa aacttgaaag
     1441 ccgcctgaat ctgttaaaat cggttgatcc cagttcataa atttatgcag tccgccagct
     1501 tcttctacta agtcttcacc agggcgtagc cataaatgat atgtattact taaaataacg
     1561 ccagcaccca tttctttcaa ctcttctggg gacattgttt ttactgtagc taatgttcct
     1621 actggcatga acattggtgt ggggaaagtt ccgtgtggcg tgatcaattc ccctaaacgc
     1681 gcgccagtat gtttttcttt cttgattaaa cgatagcgaa tggcaggttg cgtcattcat
     1741 ttccctactt tctatataaa atatcttgac aactcccacc atcataatcg tttcaagtgc
     1801 tttttgcaag ttccctcggc ttccttctac tttatttaat attctttttt ttatcctttt
     1861 tttatgaata acaaaaaaaa gaaaaacatt atgacagaaa tattttttga tacatggtat
     1921 attggtgtta ttaaagaaaa ggagggaaaa atcatgcaat cttctgcaga attaaaacgc
     1981 caagcaaaag agagtctaaa aggacgttgg ggacaagctg ttttactaaa tctaatccct
     2041 acattgattg gtattgctac tattcttctt attatgattc ctattatctt gtttgccacc
     2101 accatatatg atggatcagc gagcgtctca gaagcgacaa cttcaggagg cggtagttca
     2161 ggaggcggtg gcggaattat ttccagcatc attagcgctt tgttcatgag cggtatttct
     2221 tggacttatc ttgatatttt acgtggacaa aaagatcaga tcgaaccatt caaagatgca
     2281 tttcgtggat tcaaaggttt gtttatcggc ggagtgatca gcttagctgt gctgatctcg
     2341 atctttacaa cattatgggg tttgctatta gttattcctg gaatcattaa aagctattct
     2401 tattctcaaa gctattttat ttactacgat atcgtccaag aaacaggaca aacacctaaa
     2461 gtactggata cgatcactgc aagtcgaaaa ttgatggatg gttataaagg acaacttttc
     2521 tggttagatc tttcatttat cggctggcat atcttagcat tagcaactgc tggtattggt
     2581 tatctatggc tcagcccata catttctgca acaaaagcag cattttatga acaattgcca
     2641 aaagatattt aaaagagagt gtgacttgtg accgaggggg tggcaggaga acaccgttta
     2701 tcgagaggca gtaaaagaaa cattcagccc attagcaaaa aggaggtgtg acaacattca
     2761 tgtcacacct cctttttgta tagttccagt aaaaagaaaa tcaacttaga aacagttaat
     2821 cattcctctt cttctgcttc cggtaatcga tggaacttga tcagttcatt caccgcgatt
     2881 ggtacgatag ataaaatccc taccaagaac cagtgattca aactgatgtg tactaggccg
     2941 aatagttctt gcgtaaatgg cagcaaagca acagccgttg taatagctaa tgccagtaac
     3001 gccatttcta ccaaagcttt attggtcgct aaattgactt tgaagatcga ttggctgctg
     3061 cgtacattga acacatgcag aatcgatgaa taagctaaga tcaagaatgc aacagtttga
     3121 ccgacatgct gacttgcgtc tacccatggg ctgatagtat cgacataact acctaaataa
     3181 taaccaaaca aggttacgat ggtgaagacc cctgcattta tggcaatttt ttgcaataaa
     3241 cctcgagcaa agatcccttc atttttcgga atgggtggct gatccatgat gccgtgttcc
     3301 gctggctctc ttcctaaagc aaatcctgga agtccatcag caactacatt gacaaacagt
     3361 agttgcacag ctgtaaatgg tgctccccat cctaacagca tcgcaatcaa aacaataaag
     3421 atctccgaaa tattacagct tagcaaaaag ttgatagctt tacgaatatt ctgataaacc
     3481 gaacgtcctt gactgacagc gtctacgatc gtcgcaaagt tgtcatccgt caaaatcata
     3541 tctgcggcac tttgcgccac atctgttcct gtgatcccca tggcacaccc aacgttactt
     3601 gctttcaacg ctggtgcatc attcacacca tccccagtca tcgctacgac tgcacccgta
     3661 cgttgccaag atttgacgat ccggatcttg tcttctggcg ttacccgagc atagacagag
     3721 agagctttga cacgcgcatc taattcttcg tctgataatt gatgcagttc tgatcctgaa
     3781 agtgcttctg acggatcatt caagatacct aattctttcg ctatggcact ggcagtcacc
     3841 acatgatctc cagtgatcat gactgttttg atgccagcct ttttcgctcg cttgattgct
     3901 cctttacttt ctggacgtgg tggatcgatc attccgatca gaccaatcaa acgtaagtct
     3961 ttttccagtg cttcagaaga aatatcttgc ggttcttcgt cataagtagc ataaccaaca
     4021 gcaatcactc tcagtgcccg tttaccaaag cgatcattaa cgatcgccgc ttgttctaca
     4081 tcgcctgaac taaagcgcgg agcaagaaca tcgaatgctc ctttggtgat tgagatgtat
     4141 ttttcgccca tttggtgaac tgttgtcatc atttttcgtt cagaatcaaa aggaagctcg
     4201 tttactcgtg gatatttttc ttctaattct gcctttgtat gataattctc ttcaacagcc
     4261 cttacgatgg ctacttctgt tggattacct ttgataacaa ggtcgtcatc ttcttgctcg
     4321 accgtcacat ctgtacatag tgcagccatt ttcaatattt ccatcgcgct gttcgtcatt
     4381 gaatcttcga tattcgttac ttcatcttct ttgctccata cccggcggac gcgcatttca
     4441 ttttgtgtca gcgttcctgt tttgtctgag cagataacat tagcggttcc tagcgtttca
     4501 actgctggca actgtcggat gatcgcatgt ttcctcgcca tcttctgcac accgtatgca
     4561 agtgtcaacg taacaataac agtcaaggtt tccggtacag ctgcaacagc caaggaaatc
     4621 gaagtcatga acatctcaag gagtggttct ccttgcagtt caccaatcac aaatactaaa
     4681 gccgcagccg ctaaagcgat catactgatt cgtttgccta gctgattcaa tcggacctgt
     4741 aaaggtgttt tttgcatagt attttcgttc aaaagatccg cgatcttacc catttctgta
     4801 gccatgccaa tggcagtgac gatcgctttc ccacgacctg cagcaaccgt acatccttta
     4861 aataccatat taagttggtc acctaatgac tcatcttctt cagcaataaa tgctgcatct
     4921 ttttcaactg cttcactttc tccagtcaac gccgattctt ctacacgtaa ttgtgatgct
     4981 tcgatcaaac gggcatcggc agaaatagca tcaccggact ctaaaacaag aatatcaccc
     5041 actaccagtt gctctgactc aacagtggtc actttgccgt ctcgtatgac agttgccgtt
     5101 tgtttattca tatcttgcaa ggattccagt gctttttctg cgtttccttc ttggacaatc
     5161 gccaataccg agttgatcac aacgatagcg atgatcagca gtccttcaaa taaatcccca
     5221 tgttctgtag caaatgcggt ataaaaggaa atcaccgctg ctacaagaag gatgatcgtt
     5281 gtaaaatcgg acaagcttcg aaagaatttt ttgatcatcg attctttcgg cgcctcatcg
     5341 aatttattca atccctgctt ttttcgccgt tcatccactt cttgtgaaga caacccagta
     5401 tctggatctg cttctgttac cttctttact tcttctattg tctgcttgta ttcttccatc
     5461 ctattcctcc tttaaaatct tcgtacaaaa aaaggtactt attttactat aacatccttt
     5521 ttttatttac ttcaaataaa gggcttatcg attatttgtt aagaaagcgt aaaatttaaa
     5581 gtgcttatat ttgcaaacgc taccaaagga tgctattata tagatgtaag gaaggaggat
     5641 accatcaaat agacagaaga atttctcgat agaacagtgt aagcaaacaa agttgttaga
     5701 agtacaggtt tcacccagac atgcgtgtag agtacaaacc tataaatagt gaatgcgtat
     5761 ggatgtttgg atcacgaagt atccgaaaca agaagtaatg cgatacgtaa caattgaata
     5821 acgtaaacgt ctgaaaattg aataggaaca agagatgttt caaatgttga agatgtaacg
     5881 aaaacaaaat ttttgtttaa catgaagaaa ttcaacaccg tgctttgcat gtatgatgga
     5941 aagttacaat catttctgtt tatttgaatc cttccgaggt acaagaaagc cgagcttatc
     6001 gagagctcgg ctttttcttt gtcataaaat ctttgtaaag cttttttgtt attcaaaact
     6061 agcggctttt cataacctta tttcacgaac atcgcatctc caaaactaaa gaaacgatag
     6121 cgctcttcaa tcgcatgctg gtaagcagat aaagtcaatt ctcttcctgc aaaggcacta
     6181 actaacatga ctaaagtcga ttttggcaaa tggaaattag ttgagaaagc ttctaccact
     6241 ttgaattcat agccaggcgt aatgaaaata tcagtccatc cgctgtctgc tttgatttct
     6301 ccattaaatt tcgttccaat cgtttctaat gttcgaattg aagttgttcc gacagccacg
     6361 attttcccgc catttttacg tacttggttc aatcgctcag ccgcttcttc cgtcaaacgg
     6421 tagaattcac tatgcatatg gtgttcttca attttatcta cgctgacagg tcggaatgta
     6481 cctaacccta catgtagtgt cagatagact aattcaacac cctttgcttc gatctcatca
     6541 agtaattctt tcgtaaaatg caatccagct gttggcgctg cagcagaacc attttctttc
     6601 gcatagacag tttgatagcg atctgggtct tccagacgtt ctttgatata tggaggcaat
     6661 ggcatttctc ccaatgattc aagaatctct agaaaaattc cttcatactc aaagtcaatg
     6721 atccgtccgc cgtgttccaa ttcttctttc actactgctt gcaatctgcc atcaccaaaa
     6781 gagattcttg tcccaacttt ggcacgtttc gcaggtttga tcaatgtctc ccacgtatct
     6841 ccttgtgtat tcgttagaag aaggacttct aaatgcgcac cagtatctgg tttttctcca
     6901 tataaacgag caggcagaac tcgtgtatta ttcataacta gcgcatcacc aggctgtaac
     6961 tcttcaatga tttcatggaa atgtttatct tccatctctc cagttttccg atccaaaaca
     7021 agtaaacgag aatgatcgcg gtcttttaat ggtgtttgtg cgattaattc ttccggcaag
     7081 tcaaaatcaa aatcttctgt tgttaacata tcgtcactca ctttctatca tgtgcttcat
     7141 cattctatca tgtttttcgt tctgttggca aaatcagcac tttttcactt ctattttttg
     7201 ttttcctaaa taaaaaaact accctatttg tattttttca ttgttttagg catactagta
     7261 atagatagtt cttcattgaa aatagaaaaa gaggtggcac catgattgaa tttcaaaatg
     7321 tgtcaaaaat ttataaaggt ggaaaagtcg ctgtagagga cgtcaatctt tcatttgaaa
     7381 agggtgagtt catttgtttt atcggtacga gcggaagcgg aaaaacgacg acgatgcgga
     7441 tgatcaaccg aatgactgat ccaacacaag ggaaaatttt gattgatgga aaatccatcc
     7501 aagaaattga tccagtggaa ttgcgacgtc agatcggcta tgttatccaa agtattggat
     7561 tgatgcctca catgacgatc cgtgaaaata tcactcttgt ccaaaaattg ttgaaagtca
     7621 gtcaagaaga acggaataaa acagctgaaa aaatgattga tcttgtggaa ttgccccgtg
     7681 agatgttaga tcgctaccca catgaattat ctggtggtca gcagcagcgt attggagtcg
     7741 ttcgtgccct tgcagcgaat caagatatca tcttgatgga cgagccattc ggtgcgcttg
     7801 atccaatcac tcgtgattcc ttgcaagacc ttgtcaaaga tttacaagag cgtttgggaa
     7861 aaacgatcgt atttgtaacc catgacatgg atgaagcgat taaactagca agccgtatcg
     7921 ccatcatgag tgaaggccgt gtgatccaat ttgatacacc agaaaatatt ttacgtcatc
     7981 ctgcaaacga tttcgtagaa gagttgattg gagaagatcg cctactacaa gccaagccaa
     8041 atgcgacaag agtcggcgaa gttatgctaa atacagcaat cacgattaca ccggaaaaat
     8101 cattacaaga agcaatcaaa ctgatgaggg aaaaacgtgt agatacgttg ttagttaccg
     8161 ataattcgaa tgtattaaaa ggatttatcg atgtagaaac gttgaataaa aaacgaggaa
     8221 aagtatccag tgtcggcgac attttgaata aacatgtttt ttacgtaaaa gaatccgcct
     8281 atcttcgtga taccttgcaa cgcattttaa aacgcgggct gaaatacgtg ccggtcgttg
     8341 atgaacagaa caaagtcgta ggtatcctga ctcgtgcatc attagtggat atcgtctacg
     8401 atgtgatttg gggagaagat cccgcaacta ctgatgttgc agagtcagct actcctgaag
     8461 aatctaagga ggtctgacct atgcagcagt tttttcatca gcacggttca gaaattctct
     8521 caaaaggttt tgaacatctt tatatttcag gacttgcttt gatcattggt atcatcattg
     8581 ctgtaccgct tggtgtctta ctgacacgtt ttccaaaagc tgccacgatt gtgattggct
     8641 taaccagtgc cttgcagaca atcccttctc ttgctttatt ggcattgatg atcccgctat
     8701 ttggcgtagg taaattacct gcgattatcg cactctttat ttattcatta ttgccgattt
     8761 tacgtaatac gtacattggg atgaaaaatg tcgattggaa ttatcgggat gtggcaaaag
     8821 gcatggggat gactgagttc caatccattt tttccgttga attgcccatt gctatgccaa
     8881 cgatcatggc tggtattcgt ttagctgccg tttacgtgat tgcatgggct acacttgcct
     8941 cttatatcgg cgctggtggt ttaggcgatt tgatttttag tggactgaat aattaccaac
     9001 cagatctcat tttggcaggt acgatccctg taacaatttt agcactattg gctgattggc
     9061 ttctaggtct cttggaacac cggctgacac cgatcgcact tagagaggag aatgaagaat
     9121 gaaacgtata ctaaaaatga ccatggttct tttctctctt cttatacttg cgagttgttc
     9181 cttccctggt cttgccagca atacagatga agacaccatt tcgatcacag gtggaatcac
     9241 ttcagaagca cagatcttag caagtctagt tgccggaatg gttgaacatt atacagataa
     9301 aaataccgca atcatcaaca atctggctac tacaactatc aaccatcaag cgatgatgaa
     9361 cggagatgct tcaatttcag cggctcgcta cacaggaaca gatttaacga ctacattgaa
     9421 ccttcctcct gaaaaagatc cgaaaaaagc gtttgctacc gtgaaagatg aatttgagaa
     9481 aagatacggt caaacgtggt tcccgtccta tagttttgaa aatacgtatg tctttcttgt
     9541 tcgaaaagac accgcacaaa aataccatct atctaaagtc agcgatctga aaaatgtagc
     9601 ggatgaatta gtagctggtg tagatacttc ttggatcaat cgtaaaggag acggttatga
     9661 cggcttccaa gaaacttatg gcttctcctt caattcgata ttacctatgc agattggact
     9721 tgtttatgac gcagtcgaag caggaaaaat ggatattgtt cttggctact ctacggatgg
     9781 acggatcgcc agttatgatc tagtcatgtt ggaagatgat cgtcaattct tcccaccata
     9841 tgatgctgct ccagttgtag acaacaagat attagctgat acgcctcatt taaaagaagc
     9901 cttaaataag ttaggtcata ccatcagtac ggaaaaaatg caggaattga actacgaagc
     9961 agataataat ctagtggaac catcagtagt agcacaacgt ttcttacaag aaaaccacta
    10021 ttttgaaggg aagtgaagca attggataaa atgaatgtct ttcaacaatt tttctattat
    10081 ttccaggaaa atggctccta tatctttgca caatttatcc gtcacttcct gatttctatc
    10141 tatggtgtcc tctttgcagc agttgtcggg attccggttg ggatcatgat ttctaggaga
    10201 cgtaaattag caaactgggt gattcgtttg gcgaatatca tccagacgat cccttctcta
    10261 gccatgatct ccattttaat cattggatta ggcctgggag tcaatgtcgt gattgttact
    10321 gtatttttgt attccctctt accgatcatc aagaatacgt ataccgggat gatccaagta
    10381 gacaaaaaca tccttgatgt cggcaaaggc atggggatga ctgctagaca acgactattc
    10441 atggtagagt taccactctc tgtttctgtt atcatggcag gcataagaaa cgcgttagtt
    10501 gttgccatcg ggataaccgc gatcggtgca ttcgtcggtg ctggtggtct tggagatatc
    10561 attatccgag gaaccaatgc aacagagggc acatcgatta ttttagcagg tgcattgcca
    10621 actgcattaa tggcgatcat cacggactgg cttttaggta ttttggaaag acggctagat
    10681 ccagccagcc gtacatcacg ctgatgttat tgtaaaaaac aaaagcttct gcgaccattt
    10741 gttggtcgca aaggcttttt tagttctgtc atttttaagg caataatcca ctctgattct
    10801 tccctcctta tgggtaaaga gttgcttttc ttagctattt atccattccg tatactccaa
    10861 cttcatactg ccaacgggtt catatctgca tccaccgact aagttttttt cttatccgct
    10921 aaggcatttt gccaatccat tttcccatca ctcgtcatgg aattcatacc acatgatctc
    10981 taaatactcg tctgcttgtt ctttgaattg ggcatcaatt cttacatcct ttggcgtggc
    11041 tttcatttaa gccttttcca tcagtcctcg attgttcgat gccttcagct gcatcatcaa
    11101 aactggtgac tgccgtatcg gtaacttcga tcatctttta tacaatcggg aacttgccat
    11161 cctcatcctt ggctaaaaac atatctagcc attccagctt ctcgccatgg gaggtttgcg
    11221 gttcctaaaa ccggtacacg attcttatgg actgcatccg tcgcaactag acttggcgat
    11281 acgatgatcc ttcaccagaa gagcaccccc gtaaaccaat tgatcgatat attgccaata
    11341 agaaaagaca ttggcgtaaa agatatcgat ccctcgtgat gcattgccgt tgatactgct
    11401 attcatggcc gaaagcgcca cgactttaac ttcttccgtt tgagtgacat tcatgttcgg
    11461 caatgtcgct ttggataccc gctctgctaa aggaacgaca ctggtattgt atttcgtatc
    11521 tgtatctttt ccaaaagacc atgccaacga atcctctggg aaccaataag acgactccgg
    11581 ttgattgtcc attgcttttt tcaacatact tttggctgtt tctcgataaa cgacatttcc
    11641 aattgtcttc gcctcacttt ggcgataatc gatggtcgtt gccactgcca acactaacaa
    11701 cgatgctcca attttactga ttttccttgt cacacttctt cctccttgtt ttacataaac
    11761 ccgtcaacat tccattccta aactcaaaca atgacccttc aaaggtggag atataggaac
    11821 aaattaaaga aatcatgcaa taaatccaag aaggttggtt ttagaacaaa aaaagagtct
    11881 cctttttggg gagactcttt gttaactatc taaaaaggtt ggctcttctc acttcactca
    11941 taatgtgtcc aggcagaata aatttctaca actttatttt cctcatcaac tttgtaaacc
    12001 actcgatgct gtctattcag cctgcgtgag tatctgcctt catgtgtagg tctcagcttt
    12061 tcaaaagact gcgtgggcat atatggatct tccttcaatg tctgaatcac ttcttcaaat
    12121 tgtttcttta aattcgtttg cttgattttt cttaaatcca ctttcgcaga gtttttgatt
    12181 gcgactgtat agttactcat caaagagctt cccaatcaat atcatcgata ttcgtaaagc
    12241 tactgtcgtc tttttcgcgg tctcgtactt tatctaaagt acctgtttgc tcaagaaaaa
    12301 gggtttcttg aatcgctctc cagtcttcca gcccaatcaa tacagcattg tttccactac
    12361 gatcactaat aatctcgatt tcagtatgat tttcatttac ctctttcaat aactgataaa
    12421 aatttttacg agcagtgcta ggattcatcg ttgtattagc cattgtctct cctccttttt
    12481 ctgatataat tatagcgtac ttgattaagt acgtcaaaaa aatacaataa agcacttaaa
    12541 ttcagcaaac ttttcctctt ttttacatag agaaaaagac ccctttttag ggaggatctt
    12601 tgtccacgct cacttcttac tcctttacga ctagcggtag ccaaacgtaa agtaattggt
    12661 aacaacgtat catctttagc aaaggtcgaa tccaaagcct gtgagtctat gcaacaacac
    12721 caatacacga aggttcctcg ttgcagtgtg acttgattga tatcttgaat aacctgagga
    12781 tacgcacgaa agatttgtaa cggttattca aaactgattt tggccgtcaa gtcttctact
    12841 gcctcaaaaa tgattgcctc tttcgtctcc tgtcattcat gttcactatc gatttgatac
    12901 ttgcttagac cgttgggttt gtgaatcacc aattaaaaat ggcactgagt tatggtaaca
    12961 aaaaatggtc acttgacgag gctgagattc ctacttatga agacacatgg cacttaagag
    13021 tcgaggtaaa ggaagaagct gaaaatatag gaggctgtac attatttggc aaaggattcg
    13081 gaaattatga tcaagacatc aaaatcaaaa tgttttacag ctaaatagga agaataataa
    13141 catttcatac gctgttccca tctttaatgg cttatagagg gcacctttag ctgagaataa
    13201 gacaaaacag accctctagc ttcaattaag agctagaggg tctgtttttg attttaaaac
    13261 ttggtttatt tcttcaaatg gtagctatag gtgctgacaa cgatattttc acgccaagac
    13321 aatcgcaatc ttaaccgcaa acttgtttgg ttatggagaa tattctgcca gcgtctgttt
    13381 ggtacaaatt gaggaatcaa cacagtagtt gtatagtttt gtttcgctgc atttttactt
    13441 acgatatcta cataacggat aattggattt tcaatcgagc gataagaaga gtggacgatt
    13501 gaaaaacgga catctgggaa atgcttttta aattctgctt cgatttcttt ttctttttcg
    13561 acatcttcgt ctagcgaaac atgcatcgca acgacataat caccgatcga acgtgcgtaa
    13621 ttcaacgcac cgatatttac ccgtgtcaca ttcccgacta atacaagaac ggtattcccg
    13681 tcataatcat gtagttttgc atcattttct aaacgaagct gttctgctac ttttttataa
    13741 tgatcatgga tcttataaaa aatgaacata acgattggca taatgatgaa gaaaggccaa
    13801 atgtccccta accgatagac aaacaaaatc gcaatgattg catatgagat gaacgcaccc
    13861 gtaatattgg caatcgattt gcttaaccat ctttttcctt cttttttcca tttgacgacc
    13921 atccctgttt gagataaagc aaatggaatg aacacgccga tagagtataa cggaatcaag
    13981 cgttctgttg agccatggaa aataaacagc aatacgatcg aacctagcgc tagtgtgata
    14041 atcccattgg aatagcctag tcgatcccca cgatcttgat acatgtgagg catgaattta
    14101 tcttttgcca gattgtatgc aagaactgga aaagcagaaa aaccggtgtt agcagcaact
    14161 gctaaaatca gcgctgtagc aaattgaagg acgtaataca aaataccatg tccaaagaca
    14221 gctttaccaa tttgagacaa aacagtgact tctttttcag gaacgatccc ataccaatag
    14281 ttgataaatg tgatccccac gaagaagaaa cctaagatca atgccatcat cgtcaatgta
    14341 gcagcggcat tttttgctct tggcttctta aagaatggta cagcattact aatcgcctcc
    14401 accccagtta aagaggaaga accagaagaa aatgctcgta aaatcaaagc gatagaaaca
    14461 cccggcacag ccgctcctgg aagtgcagta gcttgcaatg gcacagcacc tgtcacgata
    14521 ttatataaac caacaacgat cagtaacgag atcacaagga taaatgtgta caccggaaat
    14581 aataggaacg atgcagattc cctcagccca cgaagattca gcatcatcag tagcaaaacg
    14641 attgtcacag aaatagcgac ttgatgaccg tagagtgctg gcaccgctga tgtgatcgct
    14701 tcagcacctg ctgagacaga tactgctact gtcaacatgt agtcgatcaa taaagagcca
    14761 ccggaaatca agccagcatt ctttccaaga ttctcactgc tgacgacata tgctcccccg
    14821 ccatgaggat aagcatggat gatttgacga tatgatagcg ttaatgaaat caataaaata
    14881 ataacaaaag cggcaatggg tagcgaatac cagatggctg cagcagacaa agcgactaaa
    14941 acgacaacga tttgttccgt accatatgca atagatgata acgcatcaga cgacaataat
    15001 gccaaagcag caaatcgggt taatttgtgc tcatcatttt cagcagattt taaaggctta
    15061 cctactaaca acctttttaa gtaatccaat ttctaaactc ctttacacac aattttactt
    15121 caagttttat agtcgaaaat ttatagtaaa caagatgtta tagtacgctc actgacctta
    15181 aaagtcaata aactttaaga aaataattgg aaaaaaacag aaaaatatca agcgcttttt
    15241 caaacgcttt aatacagcaa aaatctaaca tttttttatg ggctttttct ttgaattcta
    15301 taataaaagg atagaacttg acttagaaaa tcaagttcta tcctttttga tatcttaact
    15361 tttggaagag agatgtaagg ctgctttcta cccatcattc ttactactcg aagcatagcg
    15421 cagttgtcac aacctcttga taaacggtgt ttaaaagctt gcatctgcgg taataagccc
    15481 aaccaaatta aaaacataag aagctgtttt tatttggtcg ttcttattac ttgatgcaga
    15541 gcagcttttt tacaacctct tggtacacgg tgttcaatca gctgaccctc ggtattagct
    15601 caaaaaaacg gcaaaatatg aggaactatt tttccatttt tttctctaat actcaggtca
    15661 aaacactgat ttcacaacct ctttttttaa tgaaaaatat ggctctcatg cgccgcttct
    15721 ttgaatttcg tcattttttc ttcgatgaac ggatagattg ccgttcctac gattccaaag
    15781 acaacaaaca aaccgatcat gatccagatt gctttcgtag catttggcca ataaattcct
    15841 cctgcagatt cccgtaacaa gtttaccgca tgagtaaatg gcaagaacgg attgatcatt
    15901 tggaagaatt tccctgagac ttggatcgga tagttcccgc ctccgccaga gatcgacagt
    15961 actaagatga taatagcgat ccctttaccg acattatcga ataatgcagc caagacatag
    16021 acgatcatca tgaacgctaa ggcgatcaag acagcaaatg acacgctgta gaccggattt
    16081 tggacatcta ctcctagtaa gaaaatattc cctaatgtaa cgatcaagct ttgcgcaatc
    16141 gccattgtca aaaatgtcag catacgagca acgaattgtt ctcgtttcgt aaaattgttt
    16201 cggtctttct ttcctaaata gtattctgtc gtagcaacac ttgaaagaag aacagcgccg
    16261 acccacagac atagtgcagt ataaaacggg gtgctagctg aaccgttatt tgcgattgga
    16321 taaagcgcat tggtttgtag ctcaacaggt tgtgtaaaga agtcactttc ttttgttgcg
    16381 tctagtttca ataatttgat gacttcgcct agatcgactt cttgttcgcc ttttcggatc
    16441 gcgtctgctg ctttttggat accagctttg atatttggcc agtcattgtt gatcaagtcc
    16501 gcagcagcgt tcacagctgt ttcgacttct ggcatttttt catttactgt tgtcaatgta
    16561 ccggtgattt cattttttag tgctggccaa tcattttgga taaaatcagc ggccaatcct
    16621 aatttttgct gaatgatcgg taattcattg ttgtataggt ctgccccttc attgatgcca
    16681 ttgacgatgg tatccatatt tccattcagc attgtattgg catcatggat ttcttgtcca
    16741 attgcaggca tttctttttg atatttttct aaaattgcca ctgcattcgt tacagttgct
    16801 ttcgttgagc tgagaagcga atcaaagtcg atctggtccg ctttgctcag tgcgccttgt
    16861 gcagtcccaa tcgtatcgat gacttggtct agtagagaag tgacagtgga actgatttga
    16921 cctgtattga taccagcaat gatgccattg atattattaa caacggcatc aatttgatct
    16981 agcaattcat caatagcctt cgtgtcttca ttttgaacag ctgtgttcaa agcagatagg
    17041 cggttttgca gattgcttag caagggtttt aattgattta gctgatcaat aattggctgt
    17101 aatttcgtat ttccagcact tgtttgcaat ttagtcagga actcgaccag tttatcgatt
    17161 gcattttgtt gttgagatat tccgtcaata aagccgttca atacattgct gatgtttttc
    17221 ttttcatcat cagtcagctt accatcttca atagctgttc ttacagtatc tattaacgaa
    17281 cctgtcgttg ttgccacttg tccaattgac ttcaatgtaa catctacact gtttgtgatg
    17341 cttggtatcg catctttcag ctgagaggca cctgattttg tttggtttgc tagatcactt
    17401 gcctgatttt ctaaattttt cacatctggc aagatatctt gtacttgttg gatgatcgtc
    17461 aagccttgtt ttgcttcatc gattccttgg ttcattgtct cttggatcga agcaaaatcg
    17521 ccgtcgatct ctgctagttg cttaccggca ttttggatct ctgggatttt ttcttgcaaa
    17581 tcaaggatga tttttgcttg atctttcaac tcaggcattt tgtcattcaa tgcaacgact
    17641 ttttcaccca tctcatctac tttagggatg taatccacga attcatttgc tttattcagt
    17701 ttttccttga tttcaggtag ttgactttgt aattcgagga cttgttttgt gtagccgtcg
    17761 atcgtgtcca aattttcatc tgttgataaa atcatatctt taactttatt gatacttact
    17821 aggttcgtat cgatgtcgta acctatctca ttgaatactt tcaataaagt cgaactggct
    17881 gttttgataa attcatttgt gatttgcgat tggatcgaac ttgctccttt atcggtgatt
    17941 ttcggtgcaa tcgcattgat tttttcattt accgtatatt cgatttttgg tttcttgata
    18001 tcaccactag taaaactcag cagatcttct gagaaatcct ttggcaagta aatacctgcg
    18061 taatactttc cggaacggac tccgtcttct aattgttctt ttgaatcaac gaattgccat
    18121 cctaattgtt tatttttatg aagcgattcg atgacttgct ccccaatggc cacttcttta
    18181 ccttgaaact ctgctggctt gtctgcactg taaacagcaa ttggcagctc tccagtattt
    18241 ccatatggat cccataatgc tttgatgtta aaccacgcat atagcgaagg gatgatcatg
    18301 atcgcgatga tcagaagtgt tgcaatcggg tttttgaaaa tccgtttcca gtcaagcaaa
    18361 tataatcgaa gcgtattttt gattgactgc atagttctcc cccttcttct tttttcagct
    18421 gaattacttt accatgtatt tcatactttt ttcaatcggc ttttttcaaa aaagtcgaat
    18481 ttttggacat atcacggcaa ttgtctcatt gtaatctctt acatattgag aaataattaa
    18541 ggattacagc ctttacttta tcatgaaata aaagggaaag ataattttat gccaaagttt
    18601 ttatctatta tacgtatacg caaaaaagct aaaacatttt ttgtctttgc ccctacaaaa
    18661 atgtttcagc ttttttcgtt ttttattcct gtatttctta tttttcgaat gacaggatga
    18721 ttttcccagc aggaggatgt ccttcaacta gctgatgtgc ttggatgaca tgcttaagtg
    18781 aagcagacag attcattgaa ataaaaacat gaaaggctcc tttttggtag gctttgatta
    18841 tgccagcaaa ggcttctgca tctagatatt cctttcttgg aacaaacgat tcataaaagc
    18901 cttccttttt ttgccgcaaa tctagatctg ggaggtcatt taatgcaaca tacctgccgc
    18961 ctggtttcat gatctggatg ccggtttctc ctttgatact tcctttcgtc gcatcgatta
    19021 caaggtctgc ctgatcagca aattgcagac ctggatttgt tttatcataa gcagcaaatg
    19081 ctgaagctcc taattttttt accttttcct catttttact ggatgcactg gttaagattc
    19141 ggattccctt ttcatggaga agctggatca gacttgaccc gaccgcacct gatgctccta
    19201 gtatcattac agtatctgtc ggctggattt ccgtcaaatg gttgattaga ttataagccg
    19261 taattcctgg cgtgaccatt ccggctgctt cttcccaact cattttatct ggaatttttg
    19321 ctacttttgc tgatggcaac acgacttctt ctccgtaagt cccgccgact gcatggacag
    19381 ctactcgatc acctacatga acagttgtga catctgaacc aatctctgtc acaataccag
    19441 cgccatcatt acctggtaca taagggaatt tcaatgttcg tatctctttc atttctccta
    19501 aacgcaaggc aacatcataa ggattcacac tgaatgcttt tatttctaca cgaacatgtc
    19561 cagaaccgac ttctctggga tgagcatcga tttcttcaaa aacatcttgt gcttctccgt
    19621 aaccgttaat tgcaaatctt cgcatggtca ccatctccct ttttctttaa gtttacgttt
    19681 atgcctttat tttgacaaaa aaaatgtctt ttcgttttgg cgcatgattc aaaacaaaat
    19741 agttgtcttt tttcgcgtaa cgtgttaaat tatttatcta aaagcgtaaa gattacgatt
    19801 tacgctaata aaaaataatt ggaggaattt gttgatggct aaaaaatcaa agattgccaa
    19861 agcaaaaaaa cagcaagaaa cgattgaacg ctatgctgct ttacgccagg aattaaaagc
    19921 gcaaaaagat tacgaaggct tgcgtaaact tcctttagat gctcgtccag aacgattgaa
    19981 aaatcgtgat ttaatcgatg gacgccctcg cggatacatg cgtaaattcg ggatgtcccg
    20041 tataaaattc cgtgaattag cacataaagg tttgattcca ggagtaaaaa aagccagctg
    20101 gtaatccaag tctggctgta atgtaaagga ggagggcgca tggaccactc cttggatcat
    20161 caaaaaagat acgttcgctt cttctagcag aaatgctggt actagaagca gacgtatctt
    20221 tttttacgca cagctggtcc ttttttatat gtaaaggctg catggttact tcgctatatt
    20281 tatttagtaa caggagtcag tgcttcgata cccacactaa ttttcaatgc tcggtcaact
    20341 tctgccataa tgggtcgatc caaatggcag accttttctt tcaatcttga tttatcgatc
    20401 gttctgatct gctccaacag gataacggag tctctttcta ttcctgttcc agtagctttg
    20461 atcccaatgt gagtcggaag cttaggttta gccatttttg ccgttatggc cgcaacaatg
    20521 atcgtcgggc taaaatggtt acctagatta ttttgaataa ccaaaactgg gcgtgttccg
    20581 ccttgctccg atcccacgac tggagaaaga tcagcaaaat aaatatctcc tctttttacc
    20641 ataggatcct ccttattctt gatattctct tggtatccgc tgtcctaatc cgcaagccac
    20701 ttcatagtgg atcgtaccta attgatctgc tatatcctgc atagtgattt ctttgtctgc
    20761 atttttgccg ataagcgtca cttttgttcc gacaggaaat tcttgaggca atcggatcat
    20821 cagttgatcc atacatacac gcccaatcgt ttcacagtag ttcccttcta ccaatagaga
    20881 aaaaccctgc atctttcgtg gccagccatc tgcataaccg atcggtatcg ttccgatcca
    20941 ctctgcttca ggagtgatat atgtttctcc atagccgatt ccttcacctg ctgggagttt
    21001 tttgacttga atcaattcag aaaccagctc aagtgctggc tgcaatggat aaacttccgg
    21061 tagtgcgtgc ccagagggat taagtccgta cattgctact ccataacgaa tcatattgcc
    21121 gattgtttca tcatgccata aagccgttgc actattgctg acatgtacat atcgaggaag
    21181 ttcgggaagt tttttcagca cttctataaa gcgttctttt tgcagattcc aatatgtatc
    21241 atctgcctga tctgctgtag aaaaatgggt aaataaacct tcccattcta atacacgact
    21301 ttctttgatg aatgcagctg cctcttttac ttcttcaggt gtacaaaatc cgatacgacc
    21361 catcccagtg tctgctttta gatgaatcga aagaggcgtc tctagcttac ttttctctaa
    21421 aacagcagct gctgcttgca accattcccg tgtacctgca gttactgata aatcatgagc
    21481 aacagctaca gtcagtgcgt cgtaaggaac catattcaag ataaggatgg gctgagtaaa
    21541 tccagcttct ctcaactcga ctccttcatc taaatttgaa acacaaaagc ccgaagctcc
    21601 acctgcaact gctgcctcag ctgtttcaat agctccatga ccgtatccgt tcgcttttac
    21661 tactgcaaac aattcttttc cttgcggaag tcttgcaact tcatttgcta cattttcagt
    21721 tatagctttt ttatgtatca ctgctttcgt tgggcggtgc caagcgacaa ccatcgtttt
    21781 tccttctttc tagtcttcca aaataatttg tgcaaaagct gtttcatttg tatgagtaat
    21841 actgacccat acattgcctt catgaggtga acgcgtcaca acaggctgac cattttcatt
    21901 ttttaaaatc tcgacatctt gaaaggtaac ttttccgatc ccagttcccc aagctttaga
    21961 gaaagcctct ttgcaagcgt aacgcccgcc taaaaattct acttggcgat gaaaaggcaa
    22021 ggactggaat agtttcattt catcagaggt caagattcgt gcaatgaatt ttggtttttc
    22081 ttcgatcagc cgagtgatcc taggaagttc gactgcatcg atcccaattc cttttatcat
    22141 atgcttttct cttttcaata aatttagaac actctcattc taccaaatta taaccatttt
    22201 gccacaaata agtccacaaa taacaaaaat gaaagtttct cttaaaaaag agaaactttc
    22261 attttatgtt taatcgttat ttgaacggat tacaaatccg cgttttttat cattgtgacg
    22321 tttaccgcca tctttttggc tgttattatt tttgctataa ccgccttttg atttgtttcc
    22381 acgataatta ccgccgtcac gacgattacg gttgtttccg ccaccacgtt tgccattttt
    22441 attgtatcct tttttctgca ttggcaatgg gcgttctggt gtgatcttca ctggcactgc
    22501 atcagctgga tcttttgctg tcgtttttag caaaagtgca actaaatctt gtgcagaata
    22561 ttcttctaat aatttatctg ctgtttgcag gtatttatct agaccatttt ctgcaagttt
    22621 tgtttcgatt tgttcaactg cagcacctaa ttgacctttg aatgcttctt tttcggttgg
    22681 tggacgtaaa gtcgtcatcc gttttttcgt caagttttca atcacatgca agtagtccat
    22741 ttcatttggt gtaacgaaag tcacagacat accgcctttt ccggcacggc ctgtacgtcc
    22801 gatacggtga acgtagcttt ctggatcttg agggatatca tagttgtaca catgagtgac
    22861 acctgaaata tctaatccac gtgcagcaac gtctgtcgct acaaggatat ctaaatgacc
    22921 gctcttaaat gaacgcaata cgctcattcg tttttgttga gacaaatcgc catggattcc
    22981 ttctgcacgg taaccgcgtg cttctaatcc gcgtgccaat tcgtctacac ggcgttttgt
    23041 acgtccgaag acgattgtta attcaggtgt ttgaacatca aacaagcgag tcatgatatc
    23101 gaatttttcg tattcttttg cgcggacata gtattgatcg atcaagtcag ctgtcatttc
    23161 tttcgctttg atcttcacat gatgtggatt tttcatgaat ttaacgccaa tattcttgat
    23221 agctggcggc atcgttgctg agaaaagtaa tgtttgtctt tgatcaggaa cttgtgaaat
    23281 gattttttcg atatcttcca agaagcccat attcaacatt tcatctgctt catccaaaac
    23341 taatgtttga acggttccaa gtttcaatgt gtggcggtta atgtggtcaa gcatacgtcc
    23401 tggtgtaccc acaacgatat gtggacgatc tttcaagcca cggatctgac ggccaatatc
    23461 tgctccgccg taaacggctt gtacacgtac ttttttgtcg cgccctaaac gatataattc
    23521 ttcttgtgtt tggatagcta attcacgtgt tggtgcgata actaaacctt gtaattcgtg
    23581 acgatctgga tcgatttttt ctaacattgg taatccaaat gcagctgttt tccctgtacc
    23641 tgtctgtgct tgtccaataa cgtcttttcc agctaatgct agtggaatcg tttctgcttg
    23701 gataggcgtt gcttcttcaa aaccagcgcg ttcgactgat ttcaacaatt ctggtgataa
    23761 ttctagttct ttaaatttca aatgaatcct cctatatggt tgttaactga ggatgtagct
    23821 tttttgctac cccgtcgatt tgttcggtca aagtgtattg tttctttttg acagtggcca
    23881 cgcacgtttt tatccattct tagcccctgt ggcaccaccg aaaaatcact ttgcgaagtt
    23941 taggcattag aaaagccaaa cgggtcgcgt tcagctttat cattattatg acatctggct
    24001 tcaacaagtt gtttctatgg caaaaccagt ccaatttctc ttcatctttc ggcaataggt
    24061 tattctaaca tagtttttcg ttttctgcaa gtttacagat ttttatttca tttgctgaag
    24121 ttgttctacc acttcaaata agcccatgct gttgctgcct tttagcataa tactatctgt
    24181 tgcttcaaga ttttgtttga tagcatcaat caatttattc ttttcttttt caaaatagaa
    24241 cacaggtagt tgggggtatt tttggttcaa ttcttctttt agtgcatgca tttgttcccc
    24301 ataaaggaaa atcatggcga atttctcagg atcgatatgt tcactcattt gcgcatgcat
    24361 atttaaagac tctttcccta attccagcat atctgctaac acagcgattc tgcgtccttt
    24421 tgttggcaga tttttaaaag tatctaatac cagtcccatg gctgttggat ttgcattgta
    24481 gacatcgctc aaaatagctg caccatttcc tgccgttagc cattcggttc tgttttgagt
    24541 gatagaaaca tgggcgagcc cttggcgtat ctcaggtatg gacagtccaa agaagcgccc
    24601 gatcgtatac gcaatcaaag cattcgttac attataactt ccaggcagcg ggatcgtaaa
    24661 tgtttctcct tctactacaa aatcggtttg tgcttttcct tttgctgtca cttccgcatg
    24721 aacatcgcca tttccaatac cgaatgttgt gactgtttgt actaatgttg ccgttaaagg
    24781 tgtcagcaac ggttcatctg cgggcacaac gagtaatccg tctgatacaa gtccgtcagt
    24841 gatttccatt ttggctttag cgattccttc tcgcgaccca agattttcta tgtgtgcttc
    24901 accgatcatt gtgattgctg cagcttttgg ttgagccata agtgaaagtt ccgtgatttc
    24961 attggcgtga tccatgccca tttccaaaac gatcttttct gtatcttggg gcatgtgcaa
    25021 aatcgtgtaa ggaagaccaa tattattgtt gtaatttcct tgcgtcttat atgttttgta
    25081 tttttcagac aagacagacg cggttagatc tttcgtggtg gttttaccat tgcttcctgt
    25141 aattgcaacg acttctgcgc ctactttttc caagtaatag gttgccaatt gctgcatggc
    25201 tttcaaagga tctgctacag gaataactgg aaaatttttt ggagcttgtt caacaggcaa
    25261 gctccaaaat gaagcagatg ctccgtttgt gatcgcttgc tggataaatt catgtccatc
    25321 tcgcgtacca gcaagaggaa cgaataaact tccttcggtt atcttccggc tgtcaaattc
    25381 gacattggta atctttgtca gtgcagagca ttcgctgccc acggcttgtg caatttcttg
    25441 tattgttaga ttcattgaga tctctcctaa aaaaggttgt gaaaaaagcg tttggcctaa
    25501 acggcgggtt cgcatgactc tcacttccaa gtaaatttct ctattcgtat ttagaagcta
    25561 gaatgaacgc ggttacgcaa tcactccagc ctcttcttta tttctcttta ttcatttttt
    25621 tcaaaaaaag attgacgctg atggtatcga ttcattccta actggatcag ttcttcaatc
    25681 aaatcaccgt atttcaagcc catattttcc cataagagtg ggtacatact gaactccgta
    25741 aatcctggca tagagtttaa ttcattcagg aataattcat ttttatttgt caaaaagaaa
    25801 tcgcaccggc tcaatccgct tccacctaac atcgtgtaag ctaacttcgc gtactcttgc
    25861 gctttttgat aaacttcttc tggcacttcg gctggaatct gcatttcgat tttattattg
    25921 atatattttg cttcataatc atagaatgct acgtctttta cgacttcacc aggcaaagtc
    25981 gtccgaacgt cttcatttcc taatacagca acttcgattt cgcgcgcttc aattccttgt
    26041 tcaacgattg ctcgagaatc atactgatag gctgttgcta aagcattttg cagctcttct
    26101 cggttttctg cttttgtaat gccgacacta gaacccatat tcgccggttt gacaaacatc
    26161 ggataaagca aagaaccttc acattgatca aatacttttt taggattttc tttccattga
    26221 ttcttaagta ctggtacata aggaacttgc ggcacaccag cagcttgtaa aatatacttg
    26281 gtcatgattt tatccatggc acatgcactg gtcaatacgc ctgcgccgac ataaggcata
    26341 ttcaatgtct ctaagaagcc ttggatcgtt ccatcttccc cgtttggccc atgtaaaact
    26401 ggaaaaacga tggctccttc ttctttgatt tcgcccggat tgatcacttt tcctgtaaag
    26461 ccttcctctg tctgtccact tgggtcccat gaaagatgca agacatcttt gctggcaggt
    26521 ttttctgtta gtaatggacc tttgacccat tgtccttctt ttgtaataaa tacgagttga
    26581 acttggtaat aattataata aatggcattt aaaactgaaa atgcggaaag aatggacact
    26641 tcatgctctg cgctgcgtcc gccatatagt aaagtaatct tcaaaagatt gtcctcctgt
    26701 attctaaatc gttcagtgat ttactctcat attttagcac aaaacaaaaa taaacgaata
    26761 actt
//
LOCUS       DAYYCY010000020        26412 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000020 DAYYCY010000000
VERSION     DAYYCY010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26412
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.020"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            48..917
                     /locus_tag="ACHAZB_000699"
     CDS             48..917
                     /locus_tag="ACHAZB_000699"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFM8613393.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            1082..2353
                     /locus_tag="ACHAZB_000700"
     CDS             1082..2353
                     /locus_tag="ACHAZB_000700"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFM8613394.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            2350..3645
                     /gene="rho"
                     /locus_tag="ACHAZB_000701"
     CDS             2350..3645
                     /gene="rho"
                     /locus_tag="ACHAZB_000701"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFM8613395.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            3744..4007
                     /locus_tag="ACHAZB_000702"
     CDS             3744..4007
                     /locus_tag="ACHAZB_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFM8613396.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            4452..4901
                     /locus_tag="ACHAZB_000703"
     CDS             4452..4901
                     /locus_tag="ACHAZB_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8613397.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            4958..6376
                     /locus_tag="ACHAZB_000704"
     CDS             4958..6376
                     /locus_tag="ACHAZB_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFM8613398.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            6376..7236
                     /locus_tag="ACHAZB_000705"
     CDS             6376..7236
                     /locus_tag="ACHAZB_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFM8613399.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            7410..8183
                     /locus_tag="ACHAZB_000706"
     CDS             7410..8183
                     /locus_tag="ACHAZB_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM8613400.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            8330..8695
                     /locus_tag="ACHAZB_000707"
     CDS             8330..8695
                     /locus_tag="ACHAZB_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HFM8613401.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(8843..10051)
                     /locus_tag="ACHAZB_000708"
     CDS             complement(8843..10051)
                     /locus_tag="ACHAZB_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8613402.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(10070..10348)
                     /locus_tag="ACHAZB_000709"
     CDS             complement(10070..10348)
                     /locus_tag="ACHAZB_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8613403.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            10541..11197
                     /locus_tag="ACHAZB_000710"
     CDS             10541..11197
                     /locus_tag="ACHAZB_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFM8613404.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(11639..12436)
                     /locus_tag="ACHAZB_000711"
     CDS             complement(11639..12436)
                     /locus_tag="ACHAZB_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HFM8613405.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(12433..13617)
                     /locus_tag="ACHAZB_000712"
     CDS             complement(12433..13617)
                     /locus_tag="ACHAZB_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HFM8613406.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(13642..14889)
                     /locus_tag="ACHAZB_000713"
     CDS             complement(13642..14889)
                     /locus_tag="ACHAZB_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFM8613407.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            15104..16849
                     /gene="dnaX"
                     /locus_tag="ACHAZB_000714"
     CDS             15104..16849
                     /gene="dnaX"
                     /locus_tag="ACHAZB_000714"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFM8613408.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            16869..17183
                     /locus_tag="ACHAZB_000715"
     CDS             16869..17183
                     /locus_tag="ACHAZB_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFM8613409.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            17208..17804
                     /gene="recR"
                     /locus_tag="ACHAZB_000716"
     CDS             17208..17804
                     /gene="recR"
                     /locus_tag="ACHAZB_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFM8613410.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            17868..18512
                     /gene="tmk"
                     /locus_tag="ACHAZB_000717"
     CDS             17868..18512
                     /gene="tmk"
                     /locus_tag="ACHAZB_000717"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFM8613411.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            18527..18856
                     /locus_tag="ACHAZB_000718"
     CDS             18527..18856
                     /locus_tag="ACHAZB_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HFM8613412.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            18870..19808
                     /gene="holB"
                     /locus_tag="ACHAZB_000719"
     CDS             18870..19808
                     /gene="holB"
                     /locus_tag="ACHAZB_000719"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFM8613413.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            19844..20668
                     /locus_tag="ACHAZB_000720"
     CDS             19844..20668
                     /locus_tag="ACHAZB_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HFM8613414.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            20661..21008
                     /locus_tag="ACHAZB_000721"
     CDS             20661..21008
                     /locus_tag="ACHAZB_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HFM8613415.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            21077..21949
                     /gene="rsmI"
                     /locus_tag="ACHAZB_000722"
     CDS             21077..21949
                     /gene="rsmI"
                     /locus_tag="ACHAZB_000722"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFM8613416.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            22058..23179
                     /gene="dinB"
                     /locus_tag="ACHAZB_000723"
     CDS             22058..23179
                     /gene="dinB"
                     /locus_tag="ACHAZB_000723"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFM8613417.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(23233..23835)
                     /gene="nrdG"
                     /locus_tag="ACHAZB_000724"
     CDS             complement(23233..23835)
                     /gene="nrdG"
                     /locus_tag="ACHAZB_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HFM8613418.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYAFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(24150..26339)
                     /gene="nrdD"
                     /locus_tag="ACHAZB_000725"
     CDS             complement(24150..26339)
                     /gene="nrdD"
                     /locus_tag="ACHAZB_000725"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFM8613419.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGSFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
BASE COUNT         8462 a         4822 c         5556 g         7572 t
ORIGIN      
        1 attgtcttaa caaatttagt tttacaaaac ttaggaggaa ttttattatg ccagtagtat
       61 caggagctga atttttaaaa gctgctcgta aaggcggtta cgctgtcggc ggattcaaca
      121 caaacaactt ggaatggact caagcaatcc ttgaagcggc tgaagcaaaa caagcaccag
      181 tacttatcca aacatcaatg ggtgcagcta aatacatggg tggttacaaa atctgtaaag
      241 atttaatcac tgatttagtt gaagcaatga acatcactgt tccagttgct atccacttag
      301 accatggtga ttacgaagct gctttggaat gtatcgaatt aggctatact tcagtaatgt
      361 tcgatggttc acatctacca tttgaagaaa acttgaaatt agctaaagac gttgttgaaa
      421 aagcacatgc aaaaggtatc tcagttgaat gtgaagttgg ttcaatcggc ggagaagaag
      481 acggaatcat cggtaacggc gaattagctg atattgaaga atgcaaacaa atggttgcta
      541 caggtatcga ctacttagct tgcggtatcg gtaacatcca tggtcaatat ccagaaaact
      601 ggaaaggatt agcttttgac cacttacaag caattgctga tgctgtaggt ccagacatgc
      661 ctttagtatt acatggcgga tcaggtattc ctcaagatca aatcgaaaaa gctatctcaa
      721 tgggtatctc aaaagtaaat gttaacactg aattccaatt atcatttgct gcagctactc
      781 gtaaatatat cgaagctggt aaagataaag aaggtaaagg attcgaccct cgtaaattat
      841 tagctccagg taaagcagca atcgttgaag atgctaaatc tcatatcgac tggttcggtt
      901 cagctaacaa agcgtaagca tccacataac agatacgata tataggaatg gagaacgact
      961 tttgaagtcg ctctccattc ttttttactt aaaacggcag accatgctcg tttctttttt
     1021 ttatctatga taaaatacaa gcatatccat gaaaaaagat taataaacaa ggtggaaaca
     1081 gatgaaaaaa atcgtaatca aaggaaaccg acctttagct ggtgaagtaa cgatcagcgg
     1141 tgcaaaaaat agtgcagtcg cattgattcc agctgcaatc ttagctgatt ccccagtgat
     1201 cttggaaggt gtgccggaca tacaagatgt tcattcatta attgaaattt tagagatcat
     1261 gggcgcaact gtccattttt ccaacaacat actagaaatc gatcctcgag aagtcgtatc
     1321 gattccgatg ccgcacggaa aaatcaatag tttgcgtgct tcttattatt ttatgggaac
     1381 actgcttggt aaatttggcg aagcggtagt aggcctgcca ggtggctgtt atttaggccc
     1441 tagaccgatt gacctacacg tcaaaggatt tgaagctcta ggtgctgaag tgacaaatga
     1501 acatggtgcc atgtatttac ggacagaaaa taaaggattg cgtggcaatc ggattttcat
     1561 ggacgtggtt tcagtcggag caacgatcaa tgtgatgtta gctgctgtaa aagcaaaagg
     1621 acagacagta attgaaaacg ctgcacgaga accggaaatc atagatgtgg caactttact
     1681 gaataatatg ggggcaaaag ttcgaggagc gggtacagat gtgatccgta tcgaaggagt
     1741 cgaaacatta catggctgtc ggcatttcat gattcctgac cgcatcgaag caggaaccta
     1801 tctagcatta gcagctgcag taggaaatgg tatcaaagtg aaaaatgtga tctttgaaca
     1861 tttggaaagc tttatagcaa aattgcagga aattggtgtc catatgaaaa tcagcgaaga
     1921 tgaaatcgaa gtttacccct cgaaagaatt aaaacccgca aacatcatga cctatcctta
     1981 tccaggattt gccacagatc tgcagcagcc acttactgcg ttactattga tgactacagg
     2041 cacttcagaa atcatcgata cgatctatgc gaaacgtgtg aaccatgtgc cggaactagc
     2101 acgaatgggc gctgatatta ccgtggaagg aaatatgatc atcgtgaatg gtccaaataa
     2161 acttcacggc acagaagtcg tggcttctga tcttcgggca ggtgcttgtt tagtgattgc
     2221 tggactattg gcagaaggga caacgacgat ttacaatgta gattatatct tacgcggtta
     2281 tgatcatatt atcgagaaac taacagcctt aggggcatca attgaaatga tagaggaagt
     2341 gggagctgaa tgagcgatta tttgacaatg gcagaactag agaacaaaac gttaaaagaa
     2401 gtttatagtt atgccaaaga atttaaaatt ccttattaca gtaagatgaa taaaaaggaa
     2461 ttatctctag ctgtgattcg tgcacaagca gagaaacaag gattttatta tatggaagga
     2521 atattggata tcgttggtca agatggttac ggttttttac ggccaatcaa ttatggacca
     2581 agtgcagaag atatctatat ttctgcctca caaatcagac gattcagttt gagaaatggg
     2641 gacaaagtgg caggaaaagc ccgtccacct aaagaatcgg aacgttatta tggtttgatg
     2701 cacgtggaaa gcgttaatgg aaaagatccg gaagaagcaa aagaacgtcc gcattttcca
     2761 gccttaacac ctctttatcc gcaaagacag cttcaactgg aaacaacacc gcaaagacta
     2821 tctacccgaa tgatcgatat cttttctcca atcggttttg gtcagcgtgg attgatcgtc
     2881 gcaccgccaa aagcagggaa aacttctgta ttaaaagaaa tcgcaaatgg tatcaccgaa
     2941 aactatccgg aagtagaatt aatcgttcta ctgattgatg aacgaccgga agaagtaacg
     3001 gatctagaac gaagcgtgaa aggcgacgtg gtatcttcta catttgatct gcagccgcaa
     3061 aaccatactc gagtttctga attagtattg gaacgcgcaa tgcgtttagt cgaagataaa
     3121 cgagatgtcg ttatcttgat ggatagtatt actagacttg ctcgggccta taacttagtc
     3181 gttccaccaa gcggacgtac attgagtggc gggattgacc cagccgcatt gtataaacct
     3241 aagaaatttt tcggggcagc aagaaacatc gaagaaggtg gcagtttgac gattttagcg
     3301 accgctttag tcgatacagg aagccgtatg gatgacgtga tctatgaaga attcaaagga
     3361 actggaaatc tagaattaca actttcacgt gaattatctg aacgtcggat cttcccagcc
     3421 atcgatatca aaaaatcagg tacccgaaaa gaagaattat tgatgccaag tgaagaatta
     3481 gaagaaattt ggaaacttcg aaaaaatatg actggagatt cattagaata tacggaacaa
     3541 ctgatccgct ttttgcgtaa aacaaaaaac aatcagcaat ttttcaaaga atttcaagat
     3601 gtctcctttg gtaaaaaaaa tccgaccaga aatctgaaaa gataattgca accttgactg
     3661 aaacatgtta agatgttaat gttgtaaatc gacaaatcaa ctctggttca gcaaatggag
     3721 cagggcaaag gagcgaaaaa atcatgaaag aaaacatcca tccagattac cacccagtag
     3781 tattcatgga ttcaacaact ggtttcaaat tcttgtcagg ttcaacaaaa acttcacaag
     3841 aaacagttga atgggaagac ggtaatacat acccagtcat ccgtgtcgaa gtaacttctg
     3901 actcacatcc attctacact ggacgtcaaa aattcacaca agcagacgga cgtgtggacc
     3961 gtttcaacaa aaaatacggt ctcaaagacg caaacgctgc ggaataataa aaacgctgat
     4021 aactcaacgt ttagcagact atctgatagg gatagtctgt ttttttatgt tttttgccaa
     4081 tttaaaactt cgtttgatgc tgaaaaagtc gtaggatcat aatgcacaaa acaatgaaaa
     4141 actagtatat agttttaatt atccacttga taaaaacacc ttatattaac gaaatgaaga
     4201 ggggttattt cgaagtaaaa aaccatttac ctaaaattag aagctaagct ttcctttcaa
     4261 ggaaggcttt ttgttttgtt atagacttgc gattgtttgt gagctagatc gttcatttta
     4321 atgacttata cacgcggatg atgggtcttt ttcctctgtt cattcataat attgaacggt
     4381 acagctagcg atgctatact gacctcagtt ccaagaaaac gttaacattt tttgtggggg
     4441 agaggttttt tatgaggttt gatcaattga tcgcagaaga actgattgtt ttcgatccat
     4501 cgatagattc aaaaggatca ttatttgagc gaatagggga tgtgttgaaa aaaacaaata
     4561 aggtaagaaa tgttcaaaaa gtcgttaagg cactattcaa acgagaggag gagatctcca
     4621 caggaataga agatggattt gggattcccc atgcaaaaag caaatatgtg acaaaaccag
     4681 cactgtgttt ttttcataca gccaaattaa cggactatag cgggatagat gaaatgccaa
     4741 ttgaatatat ttttgccatc gtagttcctg aaaaatcagc agatatgcat ctagagattc
     4801 tcagcacact ctccagacga ttgatgaatg cagattttag aaagggaata aaagaagcca
     4861 ctacgccagc agatatcatc agacagatta gtcaagaatg aaaatagtag agtctttgtc
     4921 cgagtatcga tagtgaagaa aaaggaggaa aataatcatg aaaatcgttg gtgtaactgc
     4981 ttgcccgact ggaattgctc atacttatat gtcggcagaa aaattagaag taacagcaag
     5041 aaatctaaac catcaagcaa aattcgaaac acaaggtgta aaaacagaaa atcagttatc
     5101 tgaacaagaa atcaaagagg cagatgcaat cattcttgca gtagataaag aaatcgaatt
     5161 ggaccgattt gccgggaaaa aagtaaagcg tgtctctaca agccgagcaa tcaaagaacc
     5221 gcaagtagta atagacgaag cattgagaga tattggcgta ttcgttgtgt ctaatgaaaa
     5281 agaaccagct gcaaatgaga aaaagccgac aatctataat catttcatga atggtgtgaa
     5341 ttacatgctt ccatttgtga tcgcaggcgg aatcatcatc gcactgagtt ttgcctttgg
     5401 gattacagcg gctgatccca actcagcaga ctataatgtg ttagcagctg cattttcaag
     5461 gatcggagga gatacagcgt ttgccatgat ggttcctgcg ttaggcgcgg ggattgctac
     5521 atcgattgct ggaaaagcag gttttgcccc aggaattgtt gccggattac tggcctctac
     5581 aggtggttca ggttttcttg gcggaatgat tggtggcgtg ctagcagggt atatctgtga
     5641 ttttctggca aataaaatta gcgtaaaaaa agaattttct gctatttatc agctgattgt
     5701 cgtccccttt ttatcgatct taattattgg gcttttgatg gtatttgtga ttgaacaacc
     5761 gatcacatgg gtgttggatg ctttgacaaa ttggctgaat agcttgggaa atacatccgg
     5821 gctattgttc ggattactta tcgggatcat gatggcagct gatatgggtg ggccgatcaa
     5881 taaatcgact tcgactttct cgatcggttt gatgtcagct ggagtaaatg caccgattgc
     5941 tgcttgtatg gctgcaggaa tgacacctcc cctaggtatt gctttagcca ctttattatt
     6001 caaacataaa tttaccaaag aagaacggac ttcaggtcaa tcatgctgga ttttaggcgc
     6061 ttcgtatatt acagaaggtg ctataccgtt tgcggtatct gacccgatcc gcgtgatacc
     6121 tagtttgatg acaggttcag cagtcgctgc agggatttcc atggcagtag gagttacttc
     6181 aatggcacct catggaggga tttgggtgat gctaattcca aatgttatga acaatttacc
     6241 tatgtatgtc ttggcaattg catcaggtac agtcactaca gcctttatgc ttggactatt
     6301 gaaaaaaaca gtgaaagaga aaaaacaaga caagtggacg aaaaatgaaa atctaacaaa
     6361 tgaggtggaa gcgtaatgtt atatacaatg aaagagttat tgaaagtggc taaagagaat
     6421 caatttgcag taccagcctt caatatttgc agctatgata tgatgaaagc aatcatcgaa
     6481 gaagcagaga gattagaagc accagtaatc ttagaaattc atcctgatga aatcgcctat
     6541 ttaagtgatg agtttgttgc ggcagtaaga gcctatgctt atacaagtaa actgccgatc
     6601 gttatccact tggatcatgg cggtacgata gaagatgtct tacgggcgat tcgaaacggc
     6661 tattcttccg tgatgatcga tgcgtctttg aaatcattcg acgacaatgt acagattacg
     6721 aaaaaagtgg ttgcgttagc tcatgcggta aatgtctcag tggaggctga gcttggaacg
     6781 attgggaata atggttctgc tgaaggaggt gctgaaacaa tcatttatac agatcctgat
     6841 caagcgaaga tttttgtgaa agaaacagga atcgatacac ttgctgtagc aattggaaca
     6901 gcgcatggct tgtatccgaa aaacagaact ccaaaattaa atatcgactt actgaaagaa
     6961 ttgaaccaac aaatcgatct tccatttgtt ttacatggcg gttcaggtaa tccggacgca
     7021 gaagtcagcg aagcagtaaa atacggagtt gccaaagtga atttgagctc agatcttaaa
     7081 agcgtctttt tcaatgcttt gagagaaata ttgagtgaaa atccatcgat gtacgagccg
     7141 aatgcaattt atccagcagc taataaaaag gtacaagaag tggtcaaaca taaaatggaa
     7201 attttgaaca cagtaggcaa ggctagttta tactaaaaat aaaaatgtgt ggtcaagagg
     7261 gtatgacaat agtcttgtca taccctctta accacacacg atgttcagaa gtagtttttt
     7321 cgaaaaattt cttcttattt ctcgaagcca atcacttctg tcacaacctc ttttttcctg
     7381 ttactatata aagtaaatgg ggtgatttta tgttgaatga agtggagaat agacagcagg
     7441 agattgtgaa ttatctaaaa aacaaccaat ttgcaagagt caccgatttg atcgagttag
     7501 taaattatag cgaagccacg attaaacgtg accttgtttt tctggaaaaa agtggattga
     7561 tccgtagagt tcgcggtggc gccatgctta ttgacaatcg aaaaattgac gtgccttacc
     7621 tgatgaagat caccaaatta gaagaagaaa cagaaaaacg taagattgca gatacagctt
     7681 cttctttgat tcgagatgat atggtcttat tcttggattc cagtacaacc tctttacact
     7741 tagtcagaaa cctagcaaga tttgatggtt tgcagatcat tacgaatggc atcgtcacag
     7801 ctgctatgtt gagtgaatat acaacggcaa aagtatctgt attgggaggg gcaattgttc
     7861 cgaaacgagc aacaatcaac ggttcaaaag cctataacga tgcattgtca tattatgcgg
     7921 atttagcgat aatgagttgt cgcgggctag atttcagcca aggagtaaca gaaactcatg
     7981 aaggagaggc aatggtcaaa aaagctttta gaaaacaatc agaccggctt gccgttttag
     8041 ttacacagca aaaaattgca cataaattca tgcatcaagc cattgctttt catgaaatcg
     8101 attatctggt gacaagtaaa gaactgacaa aagaacagta tgcgaaagta aaggaacacc
     8161 atatcgaatg tctgtacgga taaattatta cgtgaatgtt ctaagtggct tcctcatttt
     8221 tagataggac cttatctttg ctcgcttctc tcagccgatg gtataataat gaaaaggtta
     8281 tcttgttaga taatcttact tttttatcca ggagaaagga gtgcagaaaa tgaaacatgt
     8341 gaaggcgttg gtagtgaagg caatcatgat ctgggcgatt ctgtgggtcg tgctgacagg
     8401 tttatacggt gttagcttta tggattcaac gattgttggg gtaattattg tagtcatgat
     8461 ctatgtgcta ggggatctgt tgatcttacg taaagtaggg aacatcgcag cgacgatcgc
     8521 agatgctggt tcagctgcag ttatcttgtg gctctatcta tattttatga atgatacagt
     8581 cgatatttgg atgaaagtat tgattcctgc tctattgatc ggtgtggcag aatggttctt
     8641 ccacaagtgg ttgctaagtc aaggcatcgt accagatgaa cggaaaatgg aataaatcaa
     8701 cagttaaact attcttatcg ataaatgaaa gctttaaaaa taaatagatg tttaattaag
     8761 aataatcata aactattttt actcaaaaaa ccgctcagat tattttcaga tctgaacggt
     8821 tttttttatc tattcatcaa gatcaaattt catttttcaa tgattcaatc aattcgccct
     8881 ctgcttcatg actatgtttt cggccattaa caagatagag tgctgtacat atcaatggaa
     8941 tgactccggc aatcacatac aatcctctaa atgaagtaag agattttagc atccccatca
     9001 aataaggacc aacgcctaag cctaggtcta aaccaataaa gtaggtagat aaagcaatac
     9061 tgattcgatg ttcgcggacg atttttaaac aaacagcctg tccatttgac ataaacgtac
     9121 cataacctag gccgactaat gctccagaga taagcaatgt cacactattc gtcgttacac
     9181 tcagtaaaaa taatcctgct gttaagaata aatagctagg atacatcacg tattgttctc
     9241 ctttgacatc gaaaatcctt ccagtcaatg gacgtgtaaa tgtgatgacc aaagcatata
     9301 cgacgaagaa aaatgaccca gcagcaacta agtggattac ttttgtataa gaagataaaa
     9361 atgataaaac acttgaataa gaaagaccca tcaaaaaggc aattcctgta atgaaaagtg
     9421 ctttcttttc aataaaacta ttgatattcc atgagcgaag agatattttt tgttcttcag
     9481 ataatggaat attttttaca ggaaagacaa ggcaggcaat cgtaacaaag aagaccagta
     9541 caattgaaaa ccagataatg aaacgaaaat cagtcatatt cagcaaaatc attcctaaaa
     9601 atggcccaac agcggcagct aaactagtac ttaatccata ataattgatg ccttcccctt
     9661 ttttagatgc agggatatat gcagtaacga tagcatttgt agctgtagaa actgtaccgt
     9721 agccgaatcc attcaagaat cgaactaaat acaatgcccc gatagttggc gtataaagat
     9781 atgctaatgt tgaaaggaga tagaagatcg cgccgcctct taaggtaaac ttccgaccaa
     9841 atagttctaa ctgtttcccc atgaataagc gtgccagcaa tgttccgatg atatagatcc
     9901 cagaagcaaa acctgcttgg ctcaagctgg cgtgtaaggt gtcttgcgca atgactgcca
     9961 tgatgaccat caacagataa taaatcaggt agacaataaa attgatcaat gtgattccaa
    10021 taaaatattt attaaataat ttcttttcca tgtatgttat acattcccct taagctgaat
    10081 atgcttttaa tttttgtatt tgaacttcta catcgtgata attggatgga tagcgatcaa
    10141 caagttggcg aataaataaa tctcttgtag acataaacag atttaaaaat tcttcgcatt
    10201 cgtttgttgg attctcatcc actgctttga tcaattcgat cgagttttga tacgcatcat
    10261 ttaaaaagac taaagaagct ttcatatcaa atcttgtttg atatgctgtt tctaatcgat
    10321 taatgattgt gttcatccag taattcatgt tttttccctc tattcttaaa aaattttact
    10381 tctgcagtat aacgaataaa aaaaaggaaa aaaatcgcat aagtgatgga aaaaggtact
    10441 taacaaatgt taatatcatt agtaaaatac ctttggactt taaaaagttt taaagtactg
    10501 tatgatcttt ttagatgaaa gtttggatgg gggagcatca ttggatagta gagtcttaaa
    10561 ggaatatttg gataaaaatg attttccgat tatacgcaaa ggatatcata aatatttgac
    10621 attcgaaggg atggaagatt cccatacgta tatcttgaaa gagggaacgg taaaagcaag
    10681 tgttatttca aatgatggga gagaatttaa ccttcgctat atcaaagatc ttgaaatcgt
    10741 ctctctttta aaagacgaat attcacaatt catcgattcg ccgtataata ttcgagttga
    10801 atcagaatat gcagagtttt atcgaatcaa tcgtgtgcgc ttttggcaaa atatcaacca
    10861 agacccaaag ctacaacact atgtaaaaga gtattaccgc tatcgtctgc tttattcgat
    10921 gaaaaaaatg cagcaaatgc tattaaacgg aaagctggga gctgtttgta cgcaacttta
    10981 cgaattatat gatttattcg gtgtgcggac agaagaaggg atgctgatcg atttttcgat
    11041 cacgaatgaa gaaatcgcca aattttgtgg catctcaact gctagcagtg taaacagaat
    11101 tttaaaacaa ctgaaaaaag atggtattat caccgttaaa gaccatcata tcgttatttt
    11161 gaatatggac ttgttagaag acaatattat cttttgatga acaaagaggt tgtgaaatca
    11221 gcgttttgac ctgagtatta gagaaacaaa cggaaaaata gttcctcata ttttgccgtt
    11281 ttttgagcta ataccgaggg tcagctgatt gaacaccgtt taccaagagg ttgtgagcct
    11341 gccgtttaac tctgagtcac aaggaacaat ttcaaaaaac agcttttcat gtttgatgaa
    11401 tattgtgact tgtgaccgag gagttggtag gagaacaccg tttaccaaga ggttgtgaaa
    11461 aagctgttct gcatcaagta ataagaagag ccaagtaaaa acagtctctt atgtttttaa
    11521 cttggctgga cttatcaccg tagatgtaag cttttgaacg ccgtttattt gcaacaaaag
    11581 gggctgtgac acgatttttg tcacagccct ttttttgcga acattcattt agcaattttt
    11641 agaaacttct gccgccaccg ccaaaagtcc cgccaccgct agaatgggta gtacttccgc
    11701 caccgccgct tccaggaggt gggcttttgg ggattcttct tgtcgtgaca aaagaatttg
    11761 tcagacgatc ggttttttca gttaatttga tactggattt ttctcggaat ggatatttgt
    11821 agatgcctga tttcaattga taacgcgaaa cagtcacaaa ataaaagaca agactaagga
    11881 taagagccaa aacaacagca atgatgattt ctactgtcgt caaaacctta tagcgtgtaa
    11941 ttttgccagt ttcttcgtct acacgataat gtcctcctgg aactccgtct tccacatatg
    12001 ttgctgattt agacaggtaa gcttttgcag cattgtaata ggcagcattt gtcatctgat
    12061 cataaacatc atctaagatc gaatcgattc tgctatcagt caaataatcg atcatattac
    12121 cagaagtcga aatgtagatt tcacgctgtc ccatatctaa tagcaatact gagccatttt
    12181 gatcatttcc gactgcgtct cttaagtaat tatcagcaaa gtctcttggt tcttctgaat
    12241 tagatgtagt cgttacgatc aagactcgtc ctttgatttt ttcattcagc ttttctgcct
    12301 gttgttccaa agattggatt tgatctgttg tgaataaccc agcctcatca tttaccgtag
    12361 ctgtatctgc atgagtagcc accggccagc caacacttac tagcaaaaga aagaataaaa
    12421 aaaacttttt catcatatca agtaacctcc gatcagaaag aggatcgcta gcccagcgaa
    12481 aatcccaaga gcggtcagtc ccaatttttt ctggctgatc ggcaggacac cgctgacctt
    12541 tcctgtttgt ccattcattg cgtagtagta gactttttta cttgagtcat tgcttcgata
    12601 agtaacaagc cataccggca gtaatacgta attatttact tcgtttgtga ttgaagcaga
    12661 agttcgcaca ccggtcaatg ttgtatatcc atttgcggta tcttttaaca gcttctctga
    12721 atagtctttt aattcagatt gaatctgtga tttgattgcc tcatattcga tatctctttt
    12781 ttctgcttgg aacccggcta aatactggct tttaaaatca attgctttat ctaacgggaa
    12841 aggttgcacg ccttcgacca tcttttgttg cgtatttttt gataaggcat tttttactag
    12901 ttctttaaaa gaaagcttgc ctgctcttga aacagcaaat tgcttcgttt ctgtgtattc
    12961 gatgtcacct atccgccata tgcggataac tgtaccattc gcctgcatgg caccatctac
    13021 ttcagcatct gtagcccaat aagggaaata cacacctgtc aacttatcga tttgatcctt
    13081 attgaaaaaa gctttgggaa caaaccattt tttcttggtc caagcgagaa atctgttgat
    13141 tgcttcttct ttctcgacag caaaaggaag tactttattt ggcaagaatt ctccgcttaa
    13201 acgtcccgat agtacgacgg gattatggca gtagtaacaa taagtcgcag cagttgtcgc
    13261 atcagtcacg atctctgcac cacagttggg acaaagaaaa agatctatcg ctgcggtatt
    13321 atctgcagat tcgtccgctg tatctgaaaa accgcctgcc tcgtcaaccg ctttgcgctc
    13381 ggcttcattc atatcggcaa gctgttcttg ggctgtaaaa gtaaaatctt cggtaggtga
    13441 aggttcttct ttgctttctt cgccagccat acgcgcttct ttttgctttt gctcatattg
    13501 gctgacttct tcttctgtat agatattcag gcagtattcg caatgaaatt tttggtcttt
    13561 gggatcaaaa gtcaacggac cgccgcaatt aggacattta tgtgtaagaa catccatcag
    13621 ctgcacacct ttctaaataa attaattaac ggggattcct ttaaacggtt taccacagtt
    13681 tggacagaat ttagggatgc cgttggaaag atccacgatc tcattgcatt cactgcatcg
    13741 catttgtagc ttaggctgaa cgttttctac aggtttggca gctccgcagt tggaacagaa
    13801 ttttccggaa ttttctgtcc cgcaaactgg acatgtccaa gtatccgcat ttccttgtgc
    13861 agtttgttga ttttgttttt ctgcttgttg tttgatttgt tcttggttat tttgagctgc
    13921 ttgagaaaga taagaaccat ttgcattcat tcccatacct acacccataa agccggtcat
    13981 tgctcctgct tcattttttc cggctgcttc cataccacgt gcaatggaac cttgcacgta
    14041 tccttcacgt acgctaggat cacctagcat cgccccttcg ttacgcatat tgatcagttt
    14101 cacagaatca tcggtgtaag aaatgctggc tacagccaca gaaacaatct ccatcccacg
    14161 taattcacgc caagaatcat ccaatgcagt atccatgtat ttgcttaatt ccaaactctt
    14221 agatggtaca taagaaatcc gttgaccatc tgcagacatc tgattgattg ctgattgtag
    14281 cgctgttagg aattcagcta aatactgctc attgatatca ttgatttcca cttgtgtttt
    14341 gtttttaggg attgcgtttg tataaaataa aatcgggtca acgacatgaa tagaataagt
    14401 gccatgtgcc cgtaagaata attctgcgtt atagaaattg tcaaagtagt tcaacggcgc
    14461 gcttgtaccg aatttgattc cctttatttc ttggagattg atataaaaaa cttgttgttt
    14521 ctgcggtgtg acgccgccaa atttgaatcg gtcaaaagtt tcagcaattg cttctttcag
    14581 tgttccattg aacatggaag gagctgcgtc atttttaatg gtgtaataac cttcctcagc
    14641 ggtataatcg atgatttttc caccatcgac caaaagcatc atcatgtttg gatagacatg
    14701 gacgactgtt ccgtctgtta aaacatcttc tgttccttta cgattggagc cacgtttgtc
    14761 atctttgcgg actttgacac cttttgtcat gaccgttgta tcactcatat tgtctggttc
    14821 gatgacttct agccattgat ctgccagacc gccgccaacc atacttgttg ctgctttgat
    14881 aagacccata aaaaattcct cctctgattc agctagtgcc aaacaattat tgtttattta
    14941 ttataccata ggaagatgtg aaacttctcg gactagagac ggaattgaaa tggaaaattg
    15001 cgtattgttt aaatacctga aaaagccgag aaggatagta gtttttttac ctaatatcac
    15061 gtataataag agatagtgaa ttttttaaaa aagggggatc cttatggcat atcaagcact
    15121 ttatcgagtc tggcgttcgc agcgtttcga agacatcgtt ggacaaaaag ctgtgacaca
    15181 gacactaaaa aatgctattg tttcccacaa aacctcacat gcctacttgt ttacaggacc
    15241 cagaggaacc ggaaagacga gtgcagcaaa gattttcgca aaagcaatca actgtccgaa
    15301 cagtaaagac ggtgagccat gcaacgaatg cgaaatgtgt cgatcaatta ctgctggtac
    15361 gcaagaagat gtgatcgaaa tcgatgctgc cagcaataac ggagtcgaag aaatccgatt
    15421 tatccgtgat cgggcaaatt atgcgcctac aaaggcagcg tacaaagtgt acattattga
    15481 tgaggtacat atgctttcaa caggagcttt caatgccttg ttgaaaacat tggaagagcc
    15541 aaaagaaagt gtcatattca tcttagcaac gaccgaacct cacaaaatac cagctacgat
    15601 catttcacgg actcagcgat ttgattttaa acggatcaat acggcagata ttgtcgaaca
    15661 tcttgaattt atcttgaaag aatcgactat cagctatgaa ccagctgcat tgaatgtgat
    15721 tgcccgttct gcagaaggcg ggatgcggga tgcattgagt attcttgatc aggcaatctc
    15781 gtttagtgaa ggaacaatta ctttagatga tgccatgcaa gtgacaggta gcttaactga
    15841 cgaaatgatg gacagttact tgagtgcttg cgtgaaaaaa gaagtaccag aagcattgga
    15901 aacattgaac caaattctcg cagccggcaa agaatcacgt cgctttctag aggatatgtt
    15961 acaatactgc cgtgacttgc tgatgtatca gcaagcacct aacctagttg aggaacaatc
    16021 gagtacgatc agaagtacat ttacagaatt ggcacaaact gtttcagcgg atcagctgtt
    16081 ttcaatgata aagattttga gcgatactca aaatgaaatt cgttttacca atagtgccgc
    16141 tatttatttg gaagtcgcta cagtcaaact tgcacagcta ccgtctgctt ctgttccttc
    16201 agcagatgta gcatttgcag atgatcagat agtaaaacag ctacaacaag agattaagga
    16261 actaagaaac gaaatcaagg aactacgcag taatacagga attgagaata caaaagtaca
    16321 gccggtacag caaacaaatc ataaaaaaca gacaagtacc tatcgtgtac cgacggaacg
    16381 agtatataaa gttctgcggg aagcgacaag acagcatctg aaccaagtaa aagaagtatg
    16441 ggaagacttg ctgatgagtc tatctgtcac tcagcgagca atgcttaaag caagcgaacc
    16501 agttgcagct ggacctacag gactagtgat tgcgtttgat tatgagattg tttgtcaaag
    16561 agcagcaaat gacgaagaat tgcagctttc ggtacacaac aacttaagcc gaatgatcaa
    16621 agattatgcg cctgatcctg tttatattac aagagaaagc tggccagaac ttcgaagaaa
    16681 ttatttgacg caggcacaag aagagggaac agaaaactct tttgcttctg aagactcaga
    16741 aggagaaatc gagttgcttc ctcaagaaca agaagtacca gaagaaacac ttgtagtaaa
    16801 agcagaggaa ctgtttggct ccgaagctgt tacagtggta gatgattaat aaaaaggagt
    16861 agataattat gatgcgtgga atgggcaata tgcaaggcat gatgaaacaa gtacaaaaaa
    16921 tgcaaaaaga aatggcagaa gcacaagagc tgctaaataa aacagagttt acaggtaaag
    16981 ccaccaatga attggtcaaa gcggttttta caggcgatcg tcgcatgaaa gatatccaaa
    17041 tcaatgaaga agtcgttgat cctgatgata tcgagatgct tcaggatctt gtcgtaatgg
    17101 cagtcaatga tgcgatcgca caaattgatg tggaaactga gaaaaaatta gggaaatacg
    17161 caaaaggttt gccaggattt tagttttaaa ctgaaggagt aatgaatatg caatatccag
    17221 aaccaattgc gaaactgatc gacagctaca tgaaacttcc aggaatcggt cagaaaacag
    17281 cgacaagact cgccttttat acgatcgata tgaaagatga agtagtaaat gaatttgcta
    17341 aatctttgtt aagtgtaaaa cgcgatttgc atttttgtag tatctgcgga aacatcacag
    17401 aagaagatcc ctgcgaaata tgtagaaatc cttcacgtga tcgaagcgtt atcctagtgg
    17461 tcgaagagcc gaaagatgta atggcaatgg aaaaaatgcg agaatatcac ggactttatc
    17521 atgttctcca tggtgtgtta tcaccgatgg aagggacagg tccagaagac atcaatattt
    17581 cttctctgtt gcagcggctg catgatgcag atgtaaaaga agtgattatc gcaacaaatg
    17641 caacaacaga gggagaagca acagctatgt atctctcacg tctgatcaaa cccgcaggta
    17701 tcaaagtgac tcgtctagca catggtctgt ctgtaggctc tgatatcgag tatgcagatg
    17761 aagttacttt attgaaagct gtcgaaggaa gacgtgagtt ataaatttct aattaagtgt
    17821 tttttcagat acaatataaa agcagcacaa gagatggggg caacaatgtg aacggactat
    17881 ttattacaat cgaggggcct gatggtgcag gaaaaaccag catcttgaat gaactttacc
    17941 ctcttttgaa agaagtagca aaaacagaaa tcattcaaac aagagaacca ggaggcattc
    18001 cgattgctga agagatccga gcagtgatcc ttgatccaaa aaatgatcgg atggatgaac
    18061 gtacggaagc actgctttat gcagcagcaa gaagacaaca tctagtcgaa aaggttctgc
    18121 ctgctctggc aaaagggaaa atcgttttat gcgatagatt tgtcgacagt tctcttgctt
    18181 atcaaggtgc aggccgacgc atcggcgtta ccgagattgc tcggttgaat gagtttgcta
    18241 cagaaggtac tacgccggat tttacccttt atcttgacgt agattcagac accggattaa
    18301 gaagaatcaa gaaaaacaga caaaatcaaa tcgaccgttt agattcggaa ggtttggaat
    18361 tccaccagcg tgtacgtcat gcttacttaa agctggcaga agagaatccg gaaagaatcc
    18421 acaaagttga tgcaagaaaa agctttgaag aagtgctgca aacaagttat catacaatta
    18481 ttgaacagta tccgcaattt tttgaaaact aggaaggtga acgtttatga aaattatttt
    18541 agcgattatc caagacaaag acagcaaccg tttatcaaat gaattgattg atgcaaatat
    18601 ccgtgccaca aaactatcat cgacaggcgg cttccttaag gctggaaaca gtacgtttat
    18661 cgtgggtatt gaagatgacc gtgtagaaga agcattggaa atcatcaaaa aaacgtgtga
    18721 atcaagaaag caatttgtat ctacacctgt tacactggat atttcgatgg atgggggcgt
    18781 cccttatcca gtagaagtag aagtaggcgg tgccacagtc ttcgtcttac ctgttgaagg
    18841 attccatcaa ttttaggagg agctgagcta tgaatcaaga aataaacttg gctcaaatgc
    18901 aaccgatcgt gtatcagcag cttcaacgga gttttgagca tgggcgtatc gctcatgctt
    18961 atctttttga aggggagaaa ggaacaggaa aacatgaagt aggtatatgg cttgcacagc
    19021 acctgttctg tacccaaatg aaagacaatc tgccttgtgg aaaatgcaac aattgtcagc
    19081 ggatcgaaaa gaaagaacat ccagatgttt tggttattga accggaagga caaaccatca
    19141 aagtcgatca gatcagacgt ttgcagacgg aattcagtcg cagcggttac gaatcaagaa
    19201 aaaaagtttt tttgatcaaa gaagctgaaa aaatgaatag cagtgcggca aacagtttgt
    19261 tgaaattctt ggaagagccc cctggagatt ttttagcggt gttagaaaca gatgcagtag
    19321 gaagaattct ccctactatt caatctcgtt gtcaagttct gcattttcaa gaactttcga
    19381 aagaagcact gatccatcga ttgcaagaag agcagattcc attggaaaaa gcaaaacttt
    19441 tggctttctt aacaaacagt ttaggaaaag cagttgaaat ctctcaagat gaatggttta
    19501 atgatgctaa ggacttgatt tatcaatggt tcgtttactt gcaaaagcaa gatactcaag
    19561 cctttatcta tgtccaaaaa aaattggtaa aatcatttaa agaaaaaaca caacagttta
    19621 caggattatc tgtcttgatg ttttattatc aggaagcaca gcgtcaagca gtagcaaacg
    19681 gcgaatatgg caaagtgaaa cagataaacc agataattga acggatattg atcgcagagc
    19741 agaaactacg cagcaatgtc agttttcagg ctgttgcgga acagttcgtt ttgcatacga
    19801 ttttttagct tgccgcaaag gatgatgatc aaggaggatg gaaatggtag aagtagtagg
    19861 tgtccgatat agagatgcag gtcatatcta ttattttgca ccaggagact ctgaatatgt
    19921 ttatagtgac aaggtattag tcgaatctca gcaatcctta catattgcta ctgttgtcct
    19981 tcctaaaaaa gagattgatc ccaatgacct tcctgaagct atccgtccga tcttgcataa
    20041 agcgtcacaa gctgaattag aaaaagacaa aaaaaacaaa aaagatgctt atgatgcatt
    20101 gaaaatcgca aaagaaaaag tgcagttgcg tgagttggaa atgaaaatgg tcaaagccga
    20161 atacacattt gatcgaaata aattgatttt ttctttcaca gcggacgggc gaattgactt
    20221 tagagaattg gtaaaagatc tggccgctgt atttcgtact cgtattgagc ttcgacaaat
    20281 tggtgtccgc gatgaagcaa agataatagg cggaattggt ccatgtgggc gtccattatg
    20341 ctgttcttct tttttaggcg atttcgtgcc tgtatcaatc aaaatggcaa aagatcaaag
    20401 tttatctttg aatccaacaa aaatttccgg tttatgcgga cgcttgatgt gctgtctgca
    20461 atatgagagc gaagaatacg agacagcgaa aaaagaactc cctgactttg gaaaagtaat
    20521 tgctacacct gatggtaaag ggaaagtcgt agggttgaat ctgcttagcc gtatcgttag
    20581 tattcggctg actggacggg aaaatcctgt cgaatatgaa tgggatgaat tgaaagaatt
    20641 tatccaagta ggtgagatga atggataaga gatcattata cgacggattg aatcaattag
    20701 aatcagagtt gagaaataca ttaacagaac ttgttgaaat gaagcatgct ttgcatgaga
    20761 tcgtagaaaa aaatacaact ttggagatgg aaaatcaacg tctgcgggaa cacctgcggg
    20821 aattgaacca agttgcacaa gctccagctg aaaatgtgaa gcaggaactt tctaagtctc
    20881 gtatgaatct tgaaaaaatt tatgaagaag gattccatgt ctgctacgat ttatatggat
    20941 caagaaggga caatgacgag ccttgtgcgt tttgtctaga agtgatctat cgcgaaagcg
    21001 gtcgatgacc aataacaaga ggagactgga gcggagaaat actgctccag tctttttatt
    21061 aaggagattg tgacagatgt ataatcaaaa aagtttcaag ggaaatccgg tatatgggga
    21121 tttatatctt gtaccgacgc caattggaaa tttagaagat atgagctaca gaagtgttcg
    21181 tatgctgcaa gaatccgatc ttatcgcaag tgaggatacg cgtaatacac aaaagttatt
    21241 gaatcatttt gaaatccata caccccaaaa aagtttacat gaacataatt ataaagaacg
    21301 tgtccctcaa ttgattgctt tgttaaaaga aggaaaaacg attgctcaag tcagtgacgc
    21361 aggaatgcct tccatcagcg atcctggaca tgagctagtt ttagcctgca taaaagaagg
    21421 gatttctgtg atctctatcc caggacctac agctgggatg acggctttga ttgcttcagg
    21481 gctagtgcct caaccatttt tattttatgg cttccttcca cgtaagaaaa aagaacagaa
    21541 agaaacattg aaaaagctaa aagaacagac agttacgctg attttttatg agtcacctta
    21601 tcggatcact gctacattga cgaatttttt agaaattttt ggaaatcgcc aagtcgtttt
    21661 gtgtcgggaa ttgacaaagg tccatgaaga ataccttaga ggaagtacgg aagagctact
    21721 cgcttatatc gaagaacatc cagtaaaggg tgaatgttgt ctgcttgtag aagggaattt
    21781 gttcgaaatg ccagtgacgg aagttgatga agagttggga acgttaaagg aacaagtaga
    21841 agcaaagatc gcttcaggcg aaaaaccaaa tgctgctatc aaagcagtag cgctgaaaaa
    21901 tgggctgaaa aaacaagaag tctaccgtca gtaccatgaa ttagactgag atagtataag
    21961 ttgtctataa tgaaagacta atgaggaaat cagctactaa cttttcaaat ctcttgaacg
    22021 cgaacgaatg ttcttgtata atgaataaga ggtgaggatg atggagctgc aatttccttt
    22081 acataatgat acatcaagaa aaatcattca tattgatatg gatgcttttt tcgcttcggt
    22141 tgaagaacga gatcatcctg agtttcgcgg acatccatta gtaattgcca gacaccccag
    22201 tgacactggt gggcgtggag tagtgactac agcaaattat gaagcaagaa aattcggaat
    22261 tcattcagca atgagtgcac aaaaagcgta tgagctttgt ccgcaagcca tctttaagcc
    22321 tggcgaccat cataaataca gcgatatttc caaacaagta cgggaagtat tctatcgtta
    22381 tacagatatt gtggaaccag ttagtatcga tgaagcgtat cttgatgtga ccgaaaataa
    22441 aatcaattgc aagtcagcca tcaaaatcgc ccgaatgatc caagcagata tttgggagag
    22501 tgtccagcta acatgttctg cgggagtaag ttacaataag tttttggcca aattagcatc
    22561 agactatcaa aaacctagag gaatcacggt agttgcccct caagacgctg tgccattttt
    22621 aaaagctttg ccaattgaaa aatttcatgg ggtagggaag aaaactgtac ctagaatgca
    22681 tgagctaggg atttatacag gaaaagacct atatgaatgc acagaaatga tgttgattcg
    22741 aaattttgga aaaatgggct attctcttta tcgtaaagtc agaggaatcc atgattctcc
    22801 agtaaacgtt acaagagagc gaaaatctgt gggaaaagag catacttatg ggcagccgtt
    22861 gcaaacggaa gaagcagtgc tgactcagtt gcgccaacta gctgaaaaag tagaagaagc
    22921 actgagaaga gtccaaaaac atgggaaaac agtagttttg aaggtcaggt acacggacta
    22981 ttctactgtg accaaaagag tgacattgcc agaatatatc tataaaaaag aagcactctt
    23041 ttatcaagca agccttatat gggaggaaat cttgggagta gaaaaaggaa tccgattgtt
    23101 agggattact ttgacgaatc tggacccgat gacttatgaa aacatcgtcc ttccattatg
    23161 ggaaaatcaa gaaatataaa aacagctttg agagttgaaa ccggaaatcc gttttctctc
    23221 ccaaagctgt ttttatatca agtcttgctt tttgatttgt tcataggttt ctgttgcatc
    23281 cgtacatttt tcccaaatca caacttgatt ggattctaaa gatttttgga cgtcaatgat
    23341 ccgctggttg ctacttccgc gaaattgtag ttttaggtca cgtttgctta attcaaagcg
    23401 tccgtcaaca aggatatcga tttgcgaaag gagttctaat ttatcttctg aatcttgtag
    23461 caattcatca aaggcatagc ctgtccatga ccagatatct tttgtcgtac cgaactcttt
    23521 tcggatacgt cgtacgactt gcagacaaac atctgtattc aaaaaaggtt cgccaccaag
    23581 taaagttaat ccttgcacgt aatcgttgcg caagtcagcg atgatttgtt cttctaattc
    23641 aggtgtgaat ggtttgccat atcggaaatt ttgtgcagct ttgttgaagc agccttcgca
    23701 ggcaaataag cagccactaa cgtaaagact gttgcggaca ccttcaccat cgacaaaatt
    23761 aaacggtttg tagtcagcaa tatagttttg gctgtagtct tttgctaacc attcttttgg
    23821 ttttggattt ctcataaaaa aacttctttc atttaaggtt caaagatata gctattgaag
    23881 ataaaatcta ctcgaccaca taaataagaa caaactgacc taaatccatt gttcaagaaa
    23941 gcttgttctt attttgatag agggcgttca aagatacaaa gccatcgaaa gagggtggtg
    24001 aaaaaagact tgtcatagcc tcttattctg aataaacggt gttcaaaagc ttgcatctgc
    24061 agtaataagc ccaactaaac gaaaaatatg aggaactatt tttgtttagc tattcttatt
    24121 acttgatgca ggacagcttt ttcacaacct catttcatgt gtttcacacg tgaggaaatt
    24181 tctttatggc ggccatgtac catcggacga gcttgcggat ttcctaaata gccacaagta
    24241 cgtttgacta catcacatgt ttttggatca tgatttccac attctggaca ttcgaaccct
    24301 cgttctgtcg gtttgaaatc accttcgaaa ccacattgat agcaatgatc gattggcgta
    24361 ttcgtaccaa gatacccgat tttgtcatat gcaaaatccc atacagcttc taaagctttc
    24421 ggattttgtt ggagcatagg gtattcacag tagtggatga atccacctga acaatatttt
    24481 ggataatcct tctcaaattc caatttttca aatggcgttg ggtttttacg cacatcatag
    24541 tggaaactgt ttgtataata ttctttatct gtgatatttt cgatgatgcc gaatttttct
    24601 gtatccaaac ggcagaaacg gtcagttaga ctttcacttg gagttgaata gacactgaaa
    24661 tgataaccat attcatcccc ccatgcatct gcattagctt ttaattcttt gacgatatca
    24721 agtgtgaaag cttttgcttc tgggtttgtt tcccattcgc caccgaagaa agctgaagca
    24781 acttcataca atccaatgta gcctaacgat acagtagctc gacggttttt gaataattca
    24841 ttgacagaat cggtacgtga aagacgtttc ccgaaagaac catacatata taaaattggc
    24901 gcatttgctg gaatggcttc tttacaacgt tctacacgat agagaagcgc atctttcatg
    24961 atttctaagc ggtcagacag taactgccag aatttattct gatctccttt tgattcgatc
    25021 gcgattcttg gcaaattcaa tgtaacaaca cctaaattca ttcgtccgac atttacttct
    25081 tgaccatttt cgtctttcca cccttgcaag aatgaacgac agcccatagg tactttgaaa
    25141 cttccagtta attcaacgat tttatcgtaa ttcagaatat caggatacat tcttttcgtt
    25201 gcacattcca atgctagttg tttgatgtcg taattaggat cttcagggtt taagttcaca
    25261 ccttttttca aagtgaagat cagtttaggg aagatagctg tccgcttttc actccctaat
    25321 ccattgattc gaatctgtaa aattgctttt tgaatttctc tttcaaacca gttttctcct
    25381 agtccaaatc ctaatgaagt gaaaggagtt tgtccatttg aagtaaataa ggtattgatt
    25441 tcatattcca aactttgcat agcatcgaag atatcttttt tggtttttgc ttttgcatat
    25501 tctttttggc gttctggcga atcgatccat tcttccgcgt cttttagatg ttttttatag
    25561 ttcaattctg caaaaggagc aagcaattca tctgttcggt cggcagaaca gccaccatat
    25621 tgacttgacg ctacattggc aataatctgt gagatttgtg ctgtggcggt ctggatagat
    25681 tttggcgatt cgacttcggc attgccgatt ttgaacccat tgttcagcat gcctttgaaa
    25741 tcaatcaagc agcaattcgt catcggtgta taaggatgat aatccaaatc atggtaatgg
    25801 atatcgcctt tttgatgcgc atttgctata tgtggtggca acattttcag tccgattgat
    25861 tttccaacga tcccggctgt cagatctcgc tgtgtattga aaacattact gtctttattt
    25921 gcgttttcgt tcacgacagt ttggtccttg ttgattagct tttcaatcga aaagttgata
    25981 tctgttgctt tactgcgagc aaaatctcgt ttggtacgat aattgatgta ctcttctgct
    26041 aattcatatt cgtttttttc tagtaatata tgttccacga tgttttgaat ctcatagatt
    26101 ttgacatttt cagcaaaccg gcttttgatt tcttggacaa ccaagtctgc aatcatttga
    26161 attttttcat aagtaagtgg tgataatgag ccgtgaattt tcttttctgc tttgatcagt
    26221 gcttcattga tcttttgttc atcgaaatca acatggcggc catcgcgttt gatgactttc
    26281 atctgggatg tctctggctg ctgagtaacg acagtttttt cttcataatt tactttcatt
    26341 atcttcaact cctgctaaat tgttcttgtc tatcataaaa caaaatcggg gaaggagcaa
    26401 ctaaatatcg gc
//
LOCUS       DAYYCY010000021        24524 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000021 DAYYCY010000000
VERSION     DAYYCY010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24524
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.021"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            46..423
                     /locus_tag="ACHAZB_000726"
     CDS             46..423
                     /locus_tag="ACHAZB_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8613420.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            complement(603..1898)
                     /gene="purB"
                     /locus_tag="ACHAZB_000727"
     CDS             complement(603..1898)
                     /gene="purB"
                     /locus_tag="ACHAZB_000727"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFM8613421.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1911..3035)
                     /gene="purK"
                     /locus_tag="ACHAZB_000728"
     CDS             complement(1911..3035)
                     /gene="purK"
                     /locus_tag="ACHAZB_000728"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFM8613422.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(3028..3516)
                     /gene="purE"
                     /locus_tag="ACHAZB_000729"
     CDS             complement(3028..3516)
                     /gene="purE"
                     /locus_tag="ACHAZB_000729"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFM8613423.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(3809..5116)
                     /locus_tag="ACHAZB_000730"
     CDS             complement(3809..5116)
                     /locus_tag="ACHAZB_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HFM8613424.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(5119..5700)
                     /locus_tag="ACHAZB_000731"
     CDS             complement(5119..5700)
                     /locus_tag="ACHAZB_000731"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFM8613425.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(5816..5913)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(6272..7564)
                     /locus_tag="ACHAZB_000732"
     CDS             complement(6272..7564)
                     /locus_tag="ACHAZB_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8613426.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(7561..8184)
                     /locus_tag="ACHAZB_000733"
     CDS             complement(7561..8184)
                     /locus_tag="ACHAZB_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8613427.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQPDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(8187..9023)
                     /locus_tag="ACHAZB_000734"
     CDS             complement(8187..9023)
                     /locus_tag="ACHAZB_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8613428.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(9040..9927)
                     /locus_tag="ACHAZB_000735"
     CDS             complement(9040..9927)
                     /locus_tag="ACHAZB_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8613429.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(9935..11170)
                     /locus_tag="ACHAZB_000736"
                     /pseudo
     CDS             complement(9935..11170)
                     /locus_tag="ACHAZB_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            complement(11853..12536)
                     /locus_tag="ACHAZB_000737"
     CDS             complement(11853..12536)
                     /locus_tag="ACHAZB_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFM8613430.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(12554..13171)
                     /locus_tag="ACHAZB_000738"
     CDS             complement(12554..13171)
                     /locus_tag="ACHAZB_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFM8613431.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(13181..14005)
                     /locus_tag="ACHAZB_000739"
     CDS             complement(13181..14005)
                     /locus_tag="ACHAZB_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFM8613432.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(13998..14558)
                     /locus_tag="ACHAZB_000740"
     CDS             complement(13998..14558)
                     /locus_tag="ACHAZB_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8613433.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            14732..15688
                     /locus_tag="ACHAZB_000741"
     CDS             14732..15688
                     /locus_tag="ACHAZB_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8613434.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(15993..16949)
                     /locus_tag="ACHAZB_000742"
     CDS             complement(15993..16949)
                     /locus_tag="ACHAZB_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HFM8613435.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            17053..17655
                     /locus_tag="ACHAZB_000743"
     CDS             17053..17655
                     /locus_tag="ACHAZB_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFM8613436.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(17743..19242)
                     /locus_tag="ACHAZB_000744"
     CDS             complement(17743..19242)
                     /locus_tag="ACHAZB_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFM8613437.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(19258..19782)
                     /locus_tag="ACHAZB_000745"
     CDS             complement(19258..19782)
                     /locus_tag="ACHAZB_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFM8613438.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            20080..20829
                     /locus_tag="ACHAZB_000746"
     CDS             20080..20829
                     /locus_tag="ACHAZB_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFM8613439.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            20820..21605
                     /locus_tag="ACHAZB_000747"
     CDS             20820..21605
                     /locus_tag="ACHAZB_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8613440.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDGGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            21574..22290
                     /locus_tag="ACHAZB_000748"
     CDS             21574..22290
                     /locus_tag="ACHAZB_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM8613441.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     QPLTCYELTKNGRKKLTDYC"
     gene            complement(22392..23411)
                     /locus_tag="ACHAZB_000749"
     CDS             complement(22392..23411)
                     /locus_tag="ACHAZB_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HFM8613442.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(23430..24431)
                     /locus_tag="ACHAZB_000750"
     CDS             complement(23430..24431)
                     /locus_tag="ACHAZB_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HFM8613443.1"
                     /translation="MTSTVFIILLGFVCRKKGIFSESVGKILSKVVLTVALPALAFRA
                     FMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYKGDKQTTLRVLTIFGSTTFF
                     GTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGLKMEAKNIKAMFVNPIIIAT
                     FAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPWLFQPMDYLAGLSSPLAWLA
                     IGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILAVLTATNILPVNFEALATIV
                     IMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVLTPVWIVVLQMIHGIGLFS"
BASE COUNT         7289 a         5264 c         4284 g         7687 t
ORIGIN      
        1 aattaactaa aaatttggat aatgggttcg aggaggcact tgccgatgga agatgcaata
       61 aaacaaggtc agcttttttt atcagaatta acagctgaag aacgttcctg tctatataat
      121 gatttgctgt cgatcaaaga cgtttattta tgcaataaag atactagttt ttatacattc
      181 cgaaaaatcc gccgagcaac caagcgctgt gctgaatgcc gtaaaaggac ttttctttat
      241 cagacagaac acaaaggcga actttcaatc agtcagattc atgaacagaa gcgtttcaaa
      301 aatcattatc gtatccagat cttattcaat ggacaagaga tggaatacgc ttttctcgaa
      361 aacttgctgc aacatctcca taaccagcat tttagttata atagccaagc catttacgct
      421 taattcctct attttttgta tgtaaagagg cacggtaaag aggtagccgc ttttaaaatc
      481 aggttcctat tattgataag aaaatataat aaactaaaag aagcagaacc gctaacggat
      541 ctgcttcttt tttggttatt tttgctattc tattttaatt gaattttgca actcactctt
      601 ctttattcca aacctactcg tttgaatatc acgtcaacgt ttttcaaatg atgatgataa
      661 tcaaaggcat cgtctatttc ttctttagat aataccgatg tgatggttgg atcttcttct
      721 aacagcgggc ggaactgcac ttggttgtcc caagcgtacg cagtttttgg ctgaacaaga
      781 tcataagctt cttctcttgt cattccctta tcgatcaatt tcagcataac gcgctggcta
      841 taaatcaaac caaatgttgc agacatattc cgcttcatgt tttcaggaaa gaccgttaga
      901 tttttaacga tattgccaaa acgatttaac atgtaattca ataaaatcgt ggtatcaggc
      961 aagatgattc gttctgctga agaatgagaa atatctcttt catgccataa tgtgacattt
     1021 tcataggcag tgaccatgtg tccgcgcacc actctggcta gaccagccat attttctgaa
     1081 ccgatcggat tccttttgtg cggcatagca gaagaacctt tttgaccttt cgcaaaaaat
     1141 tcttctactt cacgtgtttc ggatttttgc aatccgcgta tctctgttgc aaaacgttca
     1201 atacttgtag caataagcgc catcgctgag aaatattcag catgtagatc ccgagggaga
     1261 acttgagtag agatttcttg tggacggatc cccaattgtt tacatacata ctcttcaata
     1321 aatggcggga tatttgcgaa cgttcccact gctcctgaga tttttcctgc ttctactcct
     1381 tttgccgcat gttcaaatcg ttcgatgttc cgtttcattt ctgaatacca tgtggcaagt
     1441 ttcaatccaa aagtagttgg ttcagcatgg acaccgtgtg tccgacccat catcactgta
     1501 tatttgtgtt cttttgcttt ttcgccaatg atttccgtaa aacgtttcaa gtcttcccgt
     1561 aaaatatcat ttgcttgctt gatgagatag ccataagcag tatctacgac atccgtactt
     1621 gttagaccat aatggaccca ttttctttct tctcccaatg tttcagaaac cgctcgtgta
     1681 aacgcaacga catcatggcg tgttaatttt tcgatttcca agatccgatc gatatcaaat
     1741 gatgcatgct cacgaatttt tttcacatct tcttgcggga tttcaccaag ttctgcccaa
     1801 gcttcatctg ctaaaatctc gacttcgagc cacgcacggt atttgttctc ttccgtccag
     1861 atattcccca tttcagggcg tgtataacgt tctaacatgt tttttctcct ctaatcccaa
     1921 atattcgttt gtttgatttc ttccagcgtt tgctggatgt cttctgtcaa gatagtgata
     1981 tgtcccatct tgcgaccttt ttttgcttgt ttttttccat agaaatggaa ttgccagtct
     2041 ggcttattct caatcaagcg atagctttcc agcagttgtt ctcccaaaac attgaccatt
     2101 actgcattgc ttatcaaacg tactttttct cctaatggcc aaccacaaat acctcgaata
     2161 tgggcatcga actgactcat agaacaagct tcaatagaat aatgtccaga attgtgagga
     2221 cgaggagcta attcattcac atatagcccg cctgtttttg ttaaaaacat ttctacccct
     2281 aaaacacctg aaaggctcac tgcttctgca atcacacggg caatacgctc agcctcctcg
     2341 atcacatcct gatcaatcgc ggctggagca atcgtttcat gaagaatatt gttgtggtgg
     2401 atgttttcca cgaccggaaa agtcgtgaaa tctccttgac catttcctgc caccatgatc
     2461 gaaatctctt tttcaaacgg gatccatgct tctaaaacac aggttccttc tcttaaaagg
     2521 tccatggcag gagctagatc cgcccgactt ttcaaaacat actgcccctt gccatcatag
     2581 ccgcctcgag tggttttcaa aacacaagga tagccgatcc cgtcaatcgc atcttggata
     2641 tccgtcggac tgacgatcgt tgcataagga gcaatcacga tgttattcgt ttccaaaaaa
     2701 gatttttcta acaggcggtc ttgcgtaatc gcaagcaagt ctgtcccttg aggaataaat
     2761 gacatgggta aaatcgcatt taacgcctct acactgacgt tttcgaattc ataggtaatg
     2821 acatctgttc ttctggcaag ctctttcagc gcaaaggtgt catcatagac actttcaatg
     2881 tgccaatctg caacttgtgc ggctggacaa tcgctcacag gatccagcac acctacttta
     2941 aagcccattt cttttgcact aatcgccatc atacgaccta attgaccgcc accgacgatc
     3001 ccgatcgttg aaccaggcaa caaaggttta accaagttga tcactacttt ctataacagt
     3061 tttagccaac attgaacgtc tttcagccaa tttagcctcc aattctaaat catacatgga
     3121 aagcatctgt atcgcaagta gtccagcatt aattgctccc gcttttccga ttgctgtagt
     3181 agctacaggt actcctccag gcatttggac aatcgaaaga agcgaatcca aaccatttaa
     3241 tgttcgtgat tggaccggta caccgataac cggcaaagtt gtttttgatg cgaccatacc
     3301 cggcagatgc gcggctcccc ctgctcctgc aataatgatt ttgattcctt tttttcgagc
     3361 gttttgtgca tattcaaaca tcaaatctgg ggttcgatga gcagaaacga cctttttttc
     3421 ataagacaca ccgaattcat ccaatatttg acatgcttct ttcattgttt cccaatctga
     3481 agtactcccc ataatcacag agatttttgc tgtcattgtt tttcctccca gtttttctac
     3541 attcatattt taacaatcac tttttttgat gtcaaagaaa tttcgaatgt taacaaagaa
     3601 aaacatttta ttgttcgtgt ttattaaaga tgaaaaaaag gaatgtgaca aaagttaggt
     3661 cacattcctt tttatggata aacggtgttc aatcagctga ccctcggtat tagctcaaac
     3721 aaacggcaaa atacgaggaa ctatttttcc gtttgtttct ctaacaatca ggtcaaaacg
     3781 ctgatttcac aacctttctt ttgctctatt acttcttgaa ggcttcttcc aatatttctt
     3841 catctttttt caatccatta aagatcaaat tcaatatgat agccgtaatg ctactcatta
     3901 cgatgccatt tccagtgaac atttgcaatg tttgcggcat ttgactgaat aatgtcggcg
     3961 taacattgaa acccagccca aatccaacag aaacagcaac aatcaataga tttttatcat
     4021 tgctgaaatc aacttccaac agcatacgga ttccttggac tgccaccatc ccgaacatga
     4081 ctaacattcc tccaccaagt accggctcag ggatgatttg tgctaacgca ccgattttag
     4141 ggaataatcc taaaacaatc aagaagaatg cagagaaata gatgggtttt cttgttttga
     4201 ttcccgaaag ttgaaccaat ccgacatttt gagaaaagcc agtataaggg aatgtattga
     4261 aaataccgcc taagataacg gcaagtcctt ctgcacgata tccctttttc aattcttctt
     4321 ctccaacatt tttcccagta atgtcaccaa gtgcaaaata tacacctgtt gattctacca
     4381 tgctgaccat ggaaatgatg atcattagta aaatcgacca tacgtcgaaa gttggtttac
     4441 caaaataaaa gggttgtgga acatggaaca acggcgcatc tccaatcgcg ctcagatcaa
     4501 tcattcccat gaaagcagct aagatgcttc ctccgactaa accaatcaat accgcgattg
     4561 aacgaatgaa cccttttgcc agcatttgca cgcctacaat taggagaatc gtcacaaatg
     4621 caagtagcag atttgttttg tcaccaaaat cagctgctgt tgcattacct ccacccattt
     4681 tctcgattgc tacaggaatc aaagtcaaac caataacggt gataaccgtt cctgttacta
     4741 taggagggaa tagtttttta attttagaaa agacaccaga aactaacaca acaaagatcc
     4801 cagaagcgat aatagatccg tagatagctc ctactccttc attggttccg atcaagatca
     4861 gtggagctac tgcttggatc gcacatccta aaacgacagg caagccaatt ccgaaaaatc
     4921 ggttaactgt caattgcaat aatgtcgcta atccacacat aaagatatca attgaaatca
     4981 aataagtcat ctgttcttga ttaaagccta gtcctgtacc gatcaaaaga ggcaccgcta
     5041 ctgctcctgc atacatcgct agcagatgct gcagccctaa aaccgcagct tttccatttt
     5101 gtgtttcttt ttccacgatt atgcgtcctc ctcaagaaac tcaacttgtc cattcgtcaa
     5161 ggaagcaata cgcgcaagag aaacgactct caatcccatt tcttcaagta actgacgtcc
     5221 atcttggaaa gacttttcga tgacgatccc gattccttct acttgcgctc cagcttgctg
     5281 gcataattcg atcaatcctt ttgccgcttg tccgtttgcc aagaaatcgt cgataatcaa
     5341 tactttgtct tctgaagata aaaatttacg agaaatagaa atcgtacttg tcacttgttt
     5401 cgtaaaagaa taaacagaag ctgtcaataa ttcttcatcc atcgtcaaac tttttgcttt
     5461 tctagcgaag atcattggaa catctaattc tcttgcagca aataaagctg gtgcgatccc
     5521 agaagcttca atggtcacaa ctttcgtgat tccttgttct ttaaatactt ctgcaaaccg
     5581 ttttcccatt tgttccatca atacagggtc tacttggtga gtgacgaagc tgtcgacttt
     5641 taatacgccc tcgcctaata tacgtccgtc tttttgaata cgttcaacta attctttcac
     5701 tttatttgct cctttatcat ttattaatac cgaaataacc gcttttgtgc aaaaacctac
     5761 tatgtttctg ttaaagaaaa acaccctcca ctttatcata agaaaagaag ggtgaaatag
     5821 ccaatctttt acttatagtc cagcatttac ggtactggct agaaactgtc atccatatta
     5881 tgacgatata taagtaggta tgtaaaacta ataatagtgt aaattcattt tcttgcaact
     5941 gttttgcacg tgaaggcagt ttgccatatt tgaataaaaa atgttgtttc aaatattgaa
     6001 aacagtctaa cactactttc tgaaactgac aacgtggttt acttattttt ttgtaaaatc
     6061 taatgatttt taatgcatta ttcctttttg ttcgggttta aatagttatt tttattttct
     6121 cctatctaaa aaagcagccc acataaaaag aaggcatgac aaaaagcatg tcatagcccc
     6181 tttttccgaa taaatagtgt tcaaaagaca gcctatcggc aataaggcaa gattttataa
     6241 actacgggaa gtaatttccg taaatctatt cttattcttt taagctgaca agctcgtcaa
     6301 tcactactgc ttttcctgaa gcaatagaat gatttgctgc gattcctgtc aaaatcgaca
     6361 aaataccatc tacgtgagtt gctgcccgat tccacggatc ttcttctttt tcttcaccaa
     6421 atagatcatc taataaacgc ggatctcctc cgccatgtcc gcccgcttct tgttcgacga
     6481 ttacctcata tggctcaccg aacatcggat agacatggat ggactgaaga acggctgctc
     6541 cttcatcctt tttatctcca cctgcattga tgtaagactt ttcgatcact ttcatttcga
     6601 tccgtccttt gctgccattg aaagaaacat tgaatccttc ccacggcata tacgcgttta
     6661 gcgaatacgt caaaatcgca ccagtttcat atttgaccgt tacgcctagt gtatcttcaa
     6721 tagagatatc atcggaaaat acactgcggt cacgaatata tccactgtct ttttctgctt
     6781 ctaaatacat ttctcgcaat tcctcattgg ctgacaggtc tatagcaaat ggatcagctt
     6841 tagctagttc gcttccatag catcgactgt aaaatatttc tactccgcgt ttctccgcat
     6901 tatgcatccc ataaaagttt aatccaccga atgcaaatac cttttgggga cggctgttca
     6961 accaaaaatt cactagatca aaatgatggg tagatttatg aacaagaagc ccgccgctat
     7021 tgtgcttatt tcgatgccat cttctgaaat aatctgcccc gtgttgcgta tttaacagcc
     7081 attcgaagtg gatagaaaac acatcaccaa tcacatcact ttctatcagt tcctttatct
     7141 tcgtgtgata cggtgcataa cgataattaa aagtgactcg caggcttctt cccgtctgtt
     7201 tttggaccgt tattatttct tgagcttttt cctcatcgat agtcagaggt ttttcgcaaa
     7261 taacatcata tccttttttc attgcacgaa tgatataatc atgatgcgtt cgatctactg
     7321 acgtaataat gacttcagcc ggccgtacgt catccagtag ttgctcaaat tgtgatggca
     7381 tacatgtctt tacttttttc tgtccatact tgttcacaag tatccgattt gcataatcca
     7441 tccttgtttg agatacatca caaaaaccga ctagttcaca agtttgatga tacgtagagg
     7501 caattgcctc ataaaaaaaa cgcgctctcc caccaagtcc gacttgtaca tattttttct
     7561 tcatttaaag tcattctcct tttcttttaa gcaaaatact gtgacaaatg gcagccctgg
     7621 atacagtaat gcagtgagta aaaaatcagg ctgtatacac cagaacagtc catacaaaag
     7681 gcataaatac cacagaaacc ccttgacgtt ttttgacaac agccagatat tccttttgat
     7741 caaataaaca agactatctg ctttatcttc tttgcagata tcaattagcg tactaaaaaa
     7801 actatgggat acaaaaagca acagacacaa tagaaaagga atcagatacc aagctccaat
     7861 ctttcccact aataaatacc aatcaactgc aaaaataatg acaaacagct gaaacaacgt
     7921 aatgatgaaa cacatccgtt tgattttccc tttcctccaa catacctttt tttctgccaa
     7981 gtaatggaca gatagataat aagaaggata catcatcaaa aaataaatca gatagacaac
     8041 acgagcagac agcactaatc ctaaagtgaa caaacatcct agaactaaag cgaaaagagc
     8101 attactgata agactggtta aaaaaatccg acagacatag tagttgagct tgtaaagaaa
     8161 catctcttct attggctttt tcatattcag cccttcagac cgcttgtact gatcccctgg
     8221 acaagatatt tattgaagaa taagaaaatc agaaaaaccg gtagcagcga caacgtagac
     8281 atcgcaaaca tactcgcgta agtcgtacta gatccagcgt ctgcaaataa tttgatggca
     8341 atcgataccg tgtaagatct cggcttgttt aaataaatca atggtcccat gaagtcgtcc
     8401 catttccaat aaaactggat gatgatcgtc gtgatgatcg aaggtttcag caaaggaaaa
     8461 ataattttgg taaagacact atacttgctg catccgtcaa taatcgctga ttcatctaac
     8521 tctttgggga tgctgaccat gaattgcatg atctggtaaa tgaaaaatgc ttgtccaaaa
     8581 aaatgtggta aaatcagcgg cacatacgtc ccaaccaaat tcagccgatt gtaaataata
     8641 aactgcggaa tcaaaatcac ttgtgcagga atcatcatcg ttactaacat gacgacgaac
     8701 cagaatcttt ttcctttgaa atcgattctc gataatgcgt aagcaataca agtcgagctc
     8761 agaactgtcc ctatcgtcga aactgcagag atgacaaacg aattcctaaa gaaagtaaaa
     8821 aatgaaattc cagcaaaccc cgcccatcct tttgcgtaat tttcccattg aaaagtctct
     8881 ggaatcaaat tcagcgaacc actcataatc tcttgatccg ttttaaaaga acctgcgatc
     8941 atccataaga ccggataaat catgactaga cctaatagca aaataaaacc gtattgaaac
     9001 catttcgtta ctgtattatt catttcttcc tcctctcatt cacacaacga cccatttttt
     9061 tgatgatttg aaaatcagaa aggtgacagc tcccataatc aacagcatga tccacgaaat
     9121 agcactagca taccccatag aattaaaaga aaaagcttgg ttatacacat aaagagcata
     9181 aaagttcgtg gcatcattcg gaccaccttg ggtaatgata aatgcctgcg taaaagtcat
     9241 aaacgccgcg attgtctgca taaccaagtt atacaaaatg atcggcgaaa gaagaggtaa
     9301 cgtgatcttg taaaacgtct gccatttgtt cgctccatcg atctctgccg cttcataata
     9361 cgagcctggg atctctttga tacctgcagc aaagatgatc attgccgaac cgaattgcca
     9421 agcagacatc aaaacaagag ggatcatgac cagtttttga tcgctaaacc aggaaagcgc
     9481 aggtgcgcct aatcgttcca ggaaaatatt cactaacccg tttttagaaa acaactcccg
     9541 ccaaactaga gcgatagaaa tactgccacc gatcaaacta ggcacataat acaaagaacg
     9601 aaagaaagtc gttcctttga tattcctcgt taaaagataa gcaatcaata gaccaaagat
     9661 cagtttcaca ggtacagatg caaatacata gagaaaagtg actcggatgg agttcaaaaa
     9721 acgggcatca ctcagcatct ttatataatt atccaagccg atccatgaga ctggatttcc
     9781 caaacgataa tcggtgaacg aataataaag tgacaagatc atcggtatca ctgttatcaa
     9841 tagaaatcct aggatgaatg gagaagcaaa tacgtaaccg accacattgt cttgatccaa
     9901 gaaccgtttt agtttttctt ttttcatgcc ttccttctgc tgttacttct tcataaatga
     9961 ctttttcaac taaaagattg tattggtctt gaatttccgg attcaaagca ctatcaaaga
    10021 cattcacttc gccgttgtcc atatcggaaa tcatttctaa ataatcaaat gtcgcttgca
    10081 aagttgcatc atctgcacga gcgagctgtt ctcgaatatt cgacatgatt ggtacaccac
    10141 gttctccttt taaaatttca ttggcttctt cactgttttg aaagaaatca ataaacttag
    10201 cagcttcttc tttatgggct gagctttttg ccatactcag catttgcgaa gaacgtaaag
    10261 ggataccaga ttctccatct gcttttctgc gaggtggatt aatcaactga atctccatat
    10321 cttcaggagc ccctgacaat atttctgtca actggttact tgatagaaac accattgccg
    10381 cttctctatc cctaaccgcc tgaaaagaca gatccgtcgt acttgcagct ccaccacctg
    10441 gttctggatg ggcccctttg tctaccagat ttttacgcag ctggaaaaaa tctgttagat
    10501 agtctggatt ttcaaatccc aagctgttag atagatcttc ccgattataa aaattcaatc
    10561 ctttctcaac ttgtggtatt tgaataatac agccagctat aaaatcttcg aatcttgaaa
    10621 tagcatagat tcctaattct tcagtgattt gatagctgat ttgttcaaat tcatcccatg
    10681 tccaatttga agctggttca gggatattta attgttcaaa tatattttta ttgtaagcaa
    10741 tcgcaaacga attcaccccg ttagacagtc caactaattg cccgtcgtaa gtcgtcgttg
    10801 ctaaatagga ttcatctgca ttagaaatat caattatccc ttgctccaca tattcatcta
    10861 atggttcaat ctgattgatg tattgaggaa attgattgtt cagttggaaa atgtcccaga
    10921 tatcatctga tgctgctagt gtacctaatt ttgtgtaata tccttcataa tcataaaatt
    10981 cataatcgat cgtcacatgg ggattttttt cttcataaag ctcgattact tttactgttg
    11041 catcgtgtcg gacttgcgaa ccccaccaag ccatgttcag cgtgattttt ttgtcatctt
    11101 cttgcccaca agccgtcaag aagaataaca taacacttaa agctaagatg attctcattc
    11161 ttttcttcat tctctgcacc tctacttcca ttgttatttt tcaatatact gtatttttat
    11221 acagaacata tttattaaac gatctttttc aaggggaata aaaaccaaga cttgccttat
    11281 cgttctgcct aggatgaaca actcaatact ttcaagaaaa attagatagt gctgattctt
    11341 ctttcatcgc taaaaacact tcttgcttca atgatgggag gataattacg caaagaaaaa
    11401 aggatatcag acaactgccc aatatccttg acatagctaa ataagcatgc ttcttcgtgt
    11461 gcgctcttcc ctaaaaaaca gaaaaataac tagtaattct gtatgaaatt aggtacaata
    11521 tacacgtcga tgcacaagca ttgactatgg caacttgatt ggtcagttcc cgctgacttt
    11581 tcaagccccg acaatttagc tgctactaaa ttgtcgggtg ccttttttag tttttattcg
    11641 tttttcattc tatagaaagc gcttcaataa atcaagacat ttttttgtta gaatgctaat
    11701 aataacaaaa ggttttaact cttcttatgt acattacagg taaaaaaaca aaaaaatttc
    11761 tctttttttt gaattctgaa caaatagtac tggagtacaa aagacgagga agagccacta
    11821 tctgctcttc ctcgtctttt gattctcacc tgttattcat gattcttttt ctcagtttcc
    11881 cgatattttt ccattttacg actaaaaagc tctccgctac ttggtggcgt atatgtgatg
    11941 gcatttgctc ctgcttgaat ggtctcagca atactttctt ccgttggacc acctgtcgca
    12001 atgataggaa gttcaggata ttttttacga aaatggcgta ctgcttctgc agtttcttta
    12061 cctgcactga tattgataat cgaaatacca gctgctaact tttcatctat ttctgtatat
    12121 ttcgacgtta ccgtactgat aactggaata tcaactactc gacaaacttc ttttactgtc
    12181 tctaaaggcg taggaccgtt caaaacgacc cctaacgaac cttgcgcttc tgcgaacatg
    12241 ctcatgtagg aagaacgagc accttgtgtc aaaccaccac ctacaccgga gaaaactgga
    12301 atgtcagctg cctcaataat gcttttagta atcgctggat gaggtgtaaa aggatacaca
    12361 gcaattactg cattcgcatt tgtattacgt atgatcgcaa tatccgtagt aaagatgatc
    12421 gaacggatca attttccaaa tatcttgatc ccgcttgctt ctaaaatgat ttccggtact
    12481 cttacgatat cttttctcaa ttccgtcatg atttctggcg taaattgttc tgccatcagc
    12541 cacttctcct ttgctatctc cggtcatact gattcaattg atacgtctcg atgacctgga
    12601 tgattttttg cgcataaccg gggtctgtcg cataacctgc atcttgcaat gcttgtgcgg
    12661 cttcctgata gttggtggcc gtaatcactt tttcatacag cgcaggattc caatctactc
    12721 cttggacgaa cagttgtgta tggtcatcca tggactgttc ccatgaatca tacactttga
    12781 agtctccttg gatagttatc cattcttcgt taacaaattc ctttgtttcc aggcttactt
    12841 tcttctgatc cccataagcc ttgatcccga aaagattatt gtatttgcta gccaaggtac
    12901 tttttcccca atttgattct aatatagctt gtcctaaaat gatactagga agcactccat
    12961 aaccatcttg tagttcttgg gcatgcggaa caagccgttc aataaattgc tgatgggatt
    13021 gcgcttcatt ttcttgctga ttatcagtaa aaggagaaga cagattcttc aaggaaaaaa
    13081 caaaggctaa gagtatcact gctacaccgg caaccactgc tggccaacgg atattcttgc
    13141 gcttttgctt tttccacttt tttttcgcca tgatcaattc ctacttttca tttaattcat
    13201 tcagatattc ttctagcgtc aattggtgtt tggtgatata ctcagcgttt tctttaccga
    13261 cataacgtaa gtgccaaggt tcatatgtga tcttagttat atcttcgtgt ccttttggat
    13321 aacgaacgat aaatccatac tgcgcagcat tatctgcgat ccatttagct ccaggctgat
    13381 ctccgtaact tgcatccaat acagtagaag gataactgtt gtaccagttt tgatcgacga
    13441 catcgactgc cagacctgtg tggtgttcgc tgtatcctgg ctccgtaatc gtttctttag
    13501 taatctttgt cgcctcctct tctgatacac cttgacgact catcacatct tggacatttt
    13561 gttcgaaaac ttgttgttgg gatgatacag accggtaagc tgagaccatc actaatggaa
    13621 atccagctgc ttcagcagcc ttggcaaatg cttcatattc tggagcaatg cgtttgtcga
    13681 tcaagtatcc gttacttaat gatgccaatt gagtagactc atcaatttct ttttctaatt
    13741 tgtgatctgg accaaccaaa actagcttcc aatcgtctgt tttaacatct ggcaattgtt
    13801 tggacgtacg atcttttgtt tctttgtcct ttaccttgct gctggttgtt tgactgttct
    13861 tttctttact agcagttgtt ttggtgcttg ttcgttcgac tgcttgattg ccggcttgaa
    13921 catgcgaatc atcatcgcta aaaataacat agcccattgc tgcgatcata atcacaactg
    13981 ctccaaatcc taaaaattta ttcacttgaa aaatcctttc gttgttcttc acgttcccca
    14041 ttcgcctcat gaacgtaagt ttcttccaga ttttcattca cccaatcgag aagttcgatt
    14101 ttttctcctt cgatctgttt ttgaacatct gctggcagac gaaaattcaa aatatcatca
    14161 tagccccatt catcatattg aatacttact ttcaagtcaa ggtatctacc agcctgctga
    14221 ttatttttca aagggatcag ctcgactttt gctgctttgc tgctaagcca tttttgcgcg
    14281 atgcgtgttt tgatttgctt gtactctgca acggcaacgt ttcgtttttc gggaaaaata
    14341 atcgtttgac gcaaaatctt ttgcgttagc atccattcat aatcagtgaa aagatttttt
    14401 actttctgca tcgcttcaga tggcagattt ttctcgccat ctttccattt ttgccattgt
    14461 tctggactga tcagcaagta ttcatctaaa aaattttttt cagtttcaaa ctgttctaat
    14521 aatacactca taataagatc aatatatact tgatgcacgt ttatcccctc cttttaacta
    14581 ttctacttaa gttttgatca aaaagcacct cttaaatcgc tgctttcatt ttttttaatg
    14641 tttcaagtcc tctcctcttt attcttcgtg atttttgtta taataataga cagacaagaa
    14701 actcaatcca actatgaagg agcgttttca gatgaaaaaa tatcaatggg gaatcgttgg
    14761 tcttggtacc atcgcacatg agtttgccga aagcttcaat caagagacga gcgaactaac
    14821 agcagttgct tccagaactt cagaaaaagc ggaaaacttc gctcaccgtt acaatattcc
    14881 aaaagcttac ggaagctatc aagaaatgtt agacgatgct gaaatcgaca tcgtgtacat
    14941 tgctgtacca aatagacagc atatcgacca tatcttggca gcattggaag caggcaaaca
    15001 cgttctatgt gaaaaagcca tcacgatgaa taaaaaagaa ctggctgacg ctatgcgctt
    15061 agcagaagaa aagaatttga tcttagcaga agcgatgaca atcttcaaca tgccacttta
    15121 tcagcagtta cgctcaataa tggatacagg aaaactgggg gctttaaaaa tgatccaagc
    15181 acctttcggc agttacaaag aaccagaccc taaaaaccgg ttcttcaatc cagaattagc
    15241 tggaggagct ctgttggata tcggtacgta tgctgtatct ttcgctcgtt tctttttgag
    15301 ctctcaacct gaagtagttg cctctacaat ggttcctttt gaaacaggcg tggacgaaca
    15361 atccgtcact atcttgcgta acaaagaaaa tgaacttgct gctgtcagct tgacttttca
    15421 ggcaaaaatg ccaaaagtcg gtatcgttgc ctttgagaac ggttatgtga cgattgctga
    15481 ttacccgcgt gctgatcgtg cggagatctt atttacagat ggtacaaagg aattcatcga
    15541 aagcgggaat acatctgaag caatgaatta cgaaataaag aatatggtac acatgattga
    15601 aggtgacttg cccaatcgct ctctcttctt gacaaaagat gtcatcgaaa tcttggatca
    15661 aatgcaagtt ctatggaaaa atatgtaatt ttaaaaggtt gtgaaaaagc tgttctgcat
    15721 caagtaataa gaacagccaa acaaaaatag ctcctcctat ttttcgtttg gctggactta
    15781 ttaccgcaga ggaaagcttt tgaacaccgt ttaagaaaga ggttgtgagc ctaccgttca
    15841 gctccgagtc acaaggagca atttcaaaaa acagcttctc atgtttgttg aatattgtga
    15901 cttgtgaccg aggagttgac aggagaacac cgtttaaaga agaggttgtg aaatcagctt
    15961 tttgacctga atattagctg aattcacaac ctctattttt ggattttttg ttgggagaat
    16021 tctgccttga tttcaataaa acttttaaaa gactgcaaaa actggaacaa ttctgctcga
    16081 tcctcgaatt cagggcgact agtcggcaga ggcatttgcc gatacaatac ataaacttct
    16141 tcaatacgtg ttaaaatttg tctgccatca tttgactctg aaaatgtttc tgcagtataa
    16201 atcaataaac catagatatg ttttccataa taagctggtt gctggattct ctctagattt
    16261 tccagcatgt ctttaatcac attggcctgt gctcttctca tagagaagta agtttcgtag
    16321 tatagcggct gtctgagcca gtagttttct tgatgttctc tggcatcttc ttggctttcg
    16381 cgaatgaatg tcagtaaatg ttcgcatttg atctgcacat cttctttatt ttctgataaa
    16441 agaaattctg ccatttgtct caaaatattt cgaaattctt tttcgatctg tatttggttt
    16501 tccttcagct gcttcgtacg atctggcatg taaacatttg caagcaaagc aaatactaat
    16561 ccaatcgcca ttaatcccgc ttcattgccg atcagctgcc aagacatgtt ttcctccact
    16621 aaataatgag tgaccaaaac cgaatttact actatccctt cggttaattt gaagcgagag
    16681 gcaagcggga tgaacaggaa taaaaaaaga ctgaagccaa aaatagtata cccaacagca
    16741 aaaaaactga acagtgctgt tgccgtagct aatataaagg ctgcgattcg attcatgccc
    16801 gtacgcaatg tagagcgctt ggtatttcct acacttaata atgcaatgat ccctgcagct
    16861 ggccaataca acagcgataa gctacccgct gtgaccatcg aaaagaagac actgatcact
    16921 gttttgactg ttcgcattcc tatacgcatt tacttccccc ttttttattt taattatact
    16981 tgaataatga aaaactagct atcagacaaa agtttaggta tgatagtaaa aagaccaaaa
    17041 aggagaatct ctatgcaatt tggccgtttt cgactaggta tgcgtacgat caaaagtgcc
    17101 ttagctgttt ttttatgtat cttgtttttt catttgactc aacgaggaat cccgatgatt
    17161 gctgcattat ctgctgtttt ttctttgcgt caggacttga ctacaaccgt ttcttttgga
    17221 aagtctagga tcatcggcaa tacattaggt ggtacattag ctatcattta tttttttgtc
    17281 aaaagctatt ttcacaatga ttttttggta gaattgatct tgcttcctat tctagttatt
    17341 attgtgatcg tcgtgtcaga tggtattaac aataattccg gtatcatttc cgcaatagct
    17401 acgttgttgc tgattgcgtt gagcatccct caaggcgaat ccgctttgta tgctatccaa
    17461 cgtgtactag atacttttat agggacattc attgcaatcg gtatcaattt ctttcttcgt
    17521 cctcctgaaa cagaaaagaa ggaagaacta tcagaagatt tggtcgaact ccaaaagaaa
    17581 gaacgattgc taagaacaaa tttacaagag atagaagcta agatccaaaa acaaaagaaa
    17641 aatagcgagg actaattttc ttttgcaata aaataaaaaa atggagagaa gaactttaaa
    17701 aagtcttcct ccatttttga ttgaacggtg aagtgtacta ctttattctt ccttcatcga
    17761 gttttgttct gcctcttttg gcgcttcata catatttact tggctagtag tcccacgtgt
    17821 cgacaaattc tctacgattt ccttcaagtc aagtcctgtt gtcgctttta gtgtttcttg
    17881 agtagaagaa agcaaatttg tggcataatt tgtcactcgg ttcgctccac tggtttctcc
    17941 tgaactgctt cctgtatcga caacagagat tttttcgata tttcctagcg gctgtgctgc
    18001 ttcgcgcatc aattgcggta gcatatcgat aaccatactc aagacagctg cttcgccata
    18061 ttccttgaag gcattggcga tcttttgttt tgcttcggct tctgcttttc cttttgctag
    18121 tgctgcttcg gcttctgctt gtccagctaa acgtgtctta ttggcttccg cttctgccag
    18181 tgcttccaca cggaatcgtt ctgcttcggc ttcagtcact tctttgactt tttgagcttg
    18241 tgcttcttgt tctttagcgt accgatcggc atctgctttt ttcttcactt ccgaatcata
    18301 ttgcttttca cgacgtgtga tttctttttc ttccagttcg atttgttttt ggcgttctac
    18361 gattttaatc tgcatttcct gttcgattac ttgttgttgt gctcgggcac tttctaaatt
    18421 gtaggcttgg tctgcttttg ctttagcaac atcttgttct tgtttgtagg ttgctaattt
    18481 cagttctttt tcttttaatg attctgcgat ctctgtttgt cgctgtaatt cagctgcttg
    18541 tgattctttt tctgcttgag cttttttgat acgcgtttct tttagagctt ccgcttctgc
    18601 tatatctgca tctcttttga cttgggcgat tcttggtttc cctaaagagt caagataacc
    18661 gtttttgtct ctgacctctt tgatagtgaa agaaacgatg atcaacccca tttttgcaag
    18721 gtctacagaa gcgacttctt gtacgctttg actgaactta tcgcgatttt gatagatttc
    18781 ttctacagtc atagaaccaa gaatcgagcg caagtgtcct tctaatactt ctcttgcttc
    18841 attttccagt tcttctcttg tcttgcctaa aaactgttct gctgctgtag caatctcttc
    18901 tacagatgaa ccgattttga tgatcgaagt accatcacac atcaccggta cgccttgttc
    18961 ggtatacact tcaggggtgg agacgtccaa tttactcgat aatagactga tccgattcga
    19021 acgttgaaaa actggcaaga cgaatgcgcc acctccacgc acaattttga ttttgttatt
    19081 gctttcatca atgtgcacat ttttgcttcc taaataactt ccactgatga tcaacgcttc
    19141 atccggtttt gctgtctggt acttcgctac aaagacgatc agcaacatca gtaaaatcaa
    19201 agctgcgatt gcgatcgcga taaaaacaac tggtatttcc atttaacaca cgctccttta
    19261 agcttttatc atgctttcat aaggcatgac ataacagatt ctttccttga tctcaatgat
    19321 caatactttc gttccccgct cagctgtctg ttcttggggg ctgtaaaaac aagctggacg
    19381 actaatggaa ccggtaacac ttttcaactg aatttctccc atacctcgta cagggatcgg
    19441 tgtgatcaca acagctactt gtccttccag agttttttca gaagacgaca tcgttgattc
    19501 ggcatgtttc atcggcatca atatatagaa attaagcaaa aataccaaca ctatcgatat
    19561 gcctccacta gcaataaata aaaataaaga tgattgatct gttaaacgct cacctagaaa
    19621 accaaataag cttgtgaaag ttaaccaagg gataataagc atcggatcaa gcgggccatc
    19681 aaaatcgaat acatctccaa aaataatcaa taggactgcc aatcctgcac aaatcagcaa
    19741 tgtataccaa taaatcgtct caagtgcgat tccccctatc atatacacgc ctccttccat
    19801 ttctacgtct atcataccaa tgtttgaaaa ttctgtatag cttcttttac cgactcagtg
    19861 gtgtttttta taggaaggtt gtgtttatcg ttgacaattt gttaaatttt tgttttcatg
    19921 ttaaaatttt cacaataaat tctttcgagg tagcctatgt ttcctttttt taagaaaaaa
    19981 ccattaaagc tccagaacac gatcatcctt gtcgtttgtt tctccacatt cgtttctttg
    20041 attgtttctg ccatattgat caggaatttt attctttcga tgaaccgatc tagtgtgtca
    20101 gataaaatct ctagcattgc taaaatcgtt gccaatgata aactcatcca aaaaaagctg
    20161 cagactggtg acccgacaca tgcgattcaa ggaataacaa atgaaatcat gattgcatcg
    20221 aatttagatt ttatcgttgt catggaccct gaaggcattc gatattctca cgcagaccca
    20281 agttacatcg gtcttccttt ctcaagtcat gaagatgtga aaaaagtgat tggaggacaa
    20341 gaatttttct ctgaaaaaga aggaatgtta gggttcggct tccgctattt cgtccctatt
    20401 tattatgatc aagagattat tggtgcagtt tgtgttggct taacaaacaa cacgattgac
    20461 aatcaagtca aagatggaca aaaaatgatt gttttttcat tgattattgg acttagcgta
    20521 ggcatcatag gcgccgtttt tcttgcccaa aaaatgaaaa aagtactgct tggacttgaa
    20581 ccacaagaaa tagcagaaca gttaagtgaa aaaacgatta tcgaaaatga agtagatgaa
    20641 ggaatcttag ccatctcttc tgataaaact atcctcttga tcaatacacc tgcaaaaaac
    20701 attttctacc aaacagagca gctattggat ttcgaagtag gaaaaaaatc agtgataatc
    20761 tatatgacgt tttgtttaaa gagacgattg agcatcaaac gaccgttcgg actcaatcca
    20821 tgtatttaaa tgggatagaa actatttcta gcgttacacc tctttttagc aatgaccgtt
    20881 ttttaggagc agtcgcaact ttacgaaatc gatcagaagt aatgaaatta atggaggagc
    20941 ttggggggac gaaacaatat ataaactcca tgagagctca aacccatgaa ttcatgaata
    21001 aacttcatgt catttctggt ttgattgatt tgaagaagta tgaagaagtc acccatttta
    21061 tccagcagct aaatcaaaat tataaaaaag agattggcca agtctcaagt ctgatcaaag
    21121 tacctgcaat tgccggtttt ttattaggca aaataaacga agccaaagaa caatctgttg
    21181 aaatgctgtt agaaagcgat tcttttatcc ctaaaatgga gatggatgac tccatccatt
    21241 cacttttgca agtgttaggg aacttacttg ataatgcaaa agaagccgtg ctacaaaaac
    21301 atgaaagaaa cggacaggtc cggattcatt taaactatga cacagaagct gaagtattca
    21361 tcgtgaccgt ctgcgatggc ggtgtcggga tatcagaaga aatctcagaa aaaatattta
    21421 cgctaggttt ttcgacgaag ggaatcaatc gtgggtttgg attgaatctc atccattcaa
    21481 tcgtcaaacg ccacggtgga tttttagaat tttccaaaca aacaaatggc ggtacaatcg
    21541 tctatctaga attaccaaaa aaggaagatc aaaatgatga caaatgtcct gataatcgaa
    21601 gatgatccaa tggttgcaga attgaatcgt acttttgtgg aaagcatccc taatatgaac
    21661 gtaatcgaaa gtttcagaaa tgtagaagaa ggaaaaaaat atttcagaca aaaaaacgca
    21721 gatctgctgc tgcttgatgt atacatgcct ggcgaaagtg gtatccattt tttatcttgg
    21781 cttagacagc aaggaaatga tatcccagtg attttgatca ctgctgcaga tgatatcgaa
    21841 accataaaga atgtgatccg ctacggtgcc ttagattatt tgatcaaacc gttcactttg
    21901 caacgttttc agctggcgat cgaaaagtat cttgctttct ctcagacaat ccaaacgact
    21961 gaaaaagccg atcagaaatt gatcgatcaa gtattcaacc acaccactcc cattcacgaa
    22021 ataccatcat tcatcgattt gcctaaaggt ctgtcaaaac tgactttaaa aaaaataagt
    22081 gcatttattc taaaagaaga tactattttt tcaacaaaag aactggctca aaaaagctca
    22141 ctgtcacgta tatccgtcaa gaaatatttg aattttttag tagaacaaga tatattgaac
    22201 gaaagaatca aatatttgga aatcggccag ccgctaacct gttatgaatt gacaaagaat
    22261 gggcggaaaa agctcacaga ttactgttga gtcatcgaat tatcccatac aaaatagtcc
    22321 aagtatacag aagtaacaac tgtttacttg gactattttt aaacatttta ttgacgaata
    22381 tccatgtttt cttatcttaa ttgacgggct ttaggaagag atacctcatt tttcgttttt
    22441 ccagtttcta ttatttcacg aaagttctcg aaacttgtcg aaatcatatt tgataatgcc
    22501 tcatctgtat ttgaacctac atgcggggtt acaagaactc tagggtacat ttctactaat
    22561 tgctggatag cagggtctgg aatcgtctcc catggcttga aggatcggaa aaaaacctct
    22621 gtttcatttg caaaaacatc ggttgcaaaa ccagctaaat gattgctttt caaagctctt
    22681 aaaatagctt cattatcttg aagctctcca cgggctgtat taatcaagat gctacctttt
    22741 ttcatttgag ctaaaaatcc ttcattgatc atttgatcgt tttcacctgg gatatgaggt
    22801 acatgaatac tgacaatatc gcttttggct aataactctt caagcgagtg gaacgtcaat
    22861 acttttttag cagcttctgt ctgatgaata tcgtagccga tcactttggc tcctagtccc
    22921 ttaaacaaaa cagcttccgt caaccctatc ctgcctgttc ctatgatccc tactgtgcaa
    22981 tttctgattt cacgactaaa catttgctca tccactcgaa aatctttgaa agatgttcgg
    23041 atcgtcgtat acgcagtatt tctcaaaagc atcatggcaa aagtcaaaga taactctgct
    23101 atcgcatttg gcgaatacga tggtacacgg gctactgtca taccaaaatc agctgccgct
    23161 tgtagatcga tatgattgac tcccacagtc cgtgtaaaaa cataacgaat cccatattcc
    23221 tgcatttttg caagattggt ctcatctgct atacaatttc ccctcaacaa tacagcatca
    23281 tggtcctttg ctgacacgat attgtcatgt gtcaacagtt cttcataaag actgagatca
    23341 taatggtaaa tattcaattt ttcaaaaaaa tctatctcgt tcggacgaac accgtaacat
    23401 gcgattctca tactttcttc ctccatttct tatgaaaata aacctatgcc atgaatcatt
    23461 tgtaatacaa cgatccatac aggtgtcagt accacagcaa aaatagttga cagtaaggat
    23521 gcattggagg ctaaaagtgc ctccctatca aaactgatag cataggctgc tgccactgtt
    23581 gcagctggtg tagccatcat gataacgatt gttgccagtg cttcaaaatt gactggtaga
    23641 atattcgttg ccgtcaaaac tgctagaatc acgatattca atgcaggtac aaataccact
    23701 ttgactagac tgtaatacca tgaagttttt tcagatgcag cttttttgaa gcttacttca
    23761 cctaatgttg cgccgattgc tagccacgcc aatggtgaac ttaaaccagc taaataatcc
    23821 atcggctgaa atagccacgg tgcagtctgg tcaattcgca aaaacgctac ttggcttatc
    23881 tctccttctg cacctgtaac tgacacttgt ggcagatacc cttggaacag ccaaatgaaa
    23941 aggccggcaa aagtagcgat aatgattgga ttgacgaaca tcgctttgat atttttagct
    24001 tccatcttca agccactcat ttttatgtat ccataagaat acaaaaagat tcggtagcca
    24061 atattgaaaa ttgaggaata catcacgccg acaggaccat aaatcgcact gacgataggc
    24121 gttccgaaaa aggtcgtaga accaaagatc gtcaggacac gcaacgttgt ttgcttatcc
    24181 cctttatagc ttaagaaaaa aggttttgaa actagaataa gaataatata aataactaat
    24241 ccccaaatca agacattgat tccttgcatc agtaattctg tattgatatc ttgcatgaaa
    24301 gctcgaaatg ccagtgctgg caaagccact gttaagacga ctttcgatag aatttttcca
    24361 acagattcag aaaaaatccc cttttttcta caaacaaacc ctaataatat aataaaaact
    24421 gttgaagtga ttgcactgac aatcgacata tctgtcaaag tcgatcgaaa aatgtcaaat
    24481 aagttcatct ttctcctcct aattatgttc atttattcac aata
//
LOCUS       DAYYCY010000022        24260 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000022 DAYYCY010000000
VERSION     DAYYCY010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24260
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.022"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(55..789)
                     /locus_tag="ACHAZB_000751"
     CDS             complement(55..789)
                     /locus_tag="ACHAZB_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFM8613444.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1116..1526
                     /locus_tag="ACHAZB_000752"
     CDS             1116..1526
                     /locus_tag="ACHAZB_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFM8613445.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1519..2220
                     /locus_tag="ACHAZB_000753"
     CDS             1519..2220
                     /locus_tag="ACHAZB_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFM8613446.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2220..3806
                     /locus_tag="ACHAZB_000754"
     CDS             2220..3806
                     /locus_tag="ACHAZB_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8613447.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3825..4046
                     /locus_tag="ACHAZB_000755"
     CDS             3825..4046
                     /locus_tag="ACHAZB_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HFM8613448.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4043..4804
                     /locus_tag="ACHAZB_000756"
     CDS             4043..4804
                     /locus_tag="ACHAZB_000756"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HFM8613449.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5184..5693
                     /locus_tag="ACHAZB_000757"
     CDS             5184..5693
                     /locus_tag="ACHAZB_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFM8613450.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5768..6307)
                     /locus_tag="ACHAZB_000758"
     CDS             complement(5768..6307)
                     /locus_tag="ACHAZB_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HFM8613451.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6447..7058)
                     /gene="rpsD"
                     /locus_tag="ACHAZB_000759"
     CDS             complement(6447..7058)
                     /gene="rpsD"
                     /locus_tag="ACHAZB_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFM8613452.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7320..8171
                     /locus_tag="ACHAZB_000760"
     CDS             7320..8171
                     /locus_tag="ACHAZB_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFM8613453.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8199..8834
                     /locus_tag="ACHAZB_000761"
     CDS             8199..8834
                     /locus_tag="ACHAZB_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HFM8613454.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8854..9627
                     /locus_tag="ACHAZB_000762"
     CDS             8854..9627
                     /locus_tag="ACHAZB_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8613455.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10035..10832
                     /locus_tag="ACHAZB_000763"
     CDS             10035..10832
                     /locus_tag="ACHAZB_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HFM8613456.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10810..11223
                     /locus_tag="ACHAZB_000764"
     CDS             10810..11223
                     /locus_tag="ACHAZB_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFM8613457.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11207..13852
                     /gene="mutS"
                     /locus_tag="ACHAZB_000765"
     CDS             11207..13852
                     /gene="mutS"
                     /locus_tag="ACHAZB_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFM8613458.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13870..15978
                     /gene="mutL"
                     /locus_tag="ACHAZB_000766"
     CDS             13870..15978
                     /gene="mutL"
                     /locus_tag="ACHAZB_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFM8613459.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            16000..16560
                     /locus_tag="ACHAZB_000767"
     CDS             16000..16560
                     /locus_tag="ACHAZB_000767"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HFM8613460.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16645..18633)
                     /gene="treP"
                     /locus_tag="ACHAZB_000768"
     CDS             complement(16645..18633)
                     /gene="treP"
                     /locus_tag="ACHAZB_000768"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HFM8613461.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18856..19569
                     /gene="treR"
                     /locus_tag="ACHAZB_000769"
     CDS             18856..19569
                     /gene="treR"
                     /locus_tag="ACHAZB_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HFM8613462.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19646..20092
                     /gene="msrB"
                     /locus_tag="ACHAZB_000770"
     CDS             19646..20092
                     /gene="msrB"
                     /locus_tag="ACHAZB_000770"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFM8613463.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20262..20864
                     /gene="ruvA"
                     /locus_tag="ACHAZB_000771"
     CDS             20262..20864
                     /gene="ruvA"
                     /locus_tag="ACHAZB_000771"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFM8613464.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAIEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20877..21878
                     /gene="ruvB"
                     /locus_tag="ACHAZB_000772"
     CDS             20877..21878
                     /gene="ruvB"
                     /locus_tag="ACHAZB_000772"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFM8613465.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21907..22485
                     /locus_tag="ACHAZB_000773"
     CDS             21907..22485
                     /locus_tag="ACHAZB_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8613466.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22537..23019
                     /locus_tag="ACHAZB_000774"
     CDS             22537..23019
                     /locus_tag="ACHAZB_000774"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HFM8613467.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23136..23510
                     /locus_tag="ACHAZB_000775"
     CDS             23136..23510
                     /locus_tag="ACHAZB_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8613468.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
     gene            23940..>24260
                     /locus_tag="ACHAZB_000776"
     CDS             23940..>24260
                     /locus_tag="ACHAZB_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFM8613469.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHL"
BASE COUNT         7952 a         4359 c         5182 g         6767 t
ORIGIN      
        1 ataaaaaaac ttggtacgca atcgctgtac caagtttttt tatttagctt ttgattaagc
       61 cataaataat aatttatata agaatactgc tacgacacca gcacagatag gtgctacaac
      121 tggtacccat gcatatcccc attgagaaga acctttatgt ttcaatggaa gaagtgcgtg
      181 tacaagacgt ggagcaaagt cgcgggctgg gtttagtcca ggacctgtag gaccaccaag
      241 tgaagcaact aatgtccata caaggaaacc aagtgctaag tgagctgtac caagattatc
      301 ttggaagaat ggtgcttttg taatacctag ggcaccaaag aataaaacga atgaaccaaa
      361 gaattcattg acgaaaccat tgaatttaga accaacgctg ttgattgttg agaatgtacc
      421 taacacatgg tttacgtctt ctgtttggtc gtagtaaggt ttgtaacaag ctactacgat
      481 caattgtcca atcgctgcac caatcaattg tgcaatgata taaggaacaa cttcagccca
      541 agggaataga ccattagatg ctaaagcaat tgtaaatgct gggttgatgt ggttaccgct
      601 gattccacca aaaatcattg ctggcatcat aaccccgaat ccatacccga cagcaatcag
      661 catccagtca ctgccatatc cttttgtacc ttttaaatct acgtttgcta cagcaccgtt
      721 acctagaacg attagtaatg ctgtaccaat catttccgcc gcaagacggg tcattagtga
      781 gtaatccata aatacctcca aaagttataa aaatgcaaaa aaaaaagaaa catcaacacg
      841 aaaagcatcg gtgcagtgtt tcgtgtcgga aggatttctg cagtcttaac aaatgtgaat
      901 aaaaaaatga attccattac ttataagaaa ccagaccaac agtaaaactc caactctttt
      961 taaaagttct ccacaaatca ccaagcttaa ctataaatat accaaaaaaa aaacgttttt
     1021 tttctcaaaa attgtatctt agaaacagca tgttttgaaa ctttcagaaa aggtttgtta
     1081 gtaaaatgaa agctatacgt aaggagtgtt gtttgatgaa attttttcaa gagtttttca
     1141 ttattatttt cttttctgta ttaggagaag gcatacggat ttttgctcat ttacctattc
     1201 ctggaagtat cttaggtatt ctttttttat ttttagcatt tgaaatgcat cttgttgatc
     1261 ctgaaaaaat cggagcaact ggtgattttt tattgaataa tctagccatt ttatttgttc
     1321 ctgctggtgt aggtttattg gaatattttg atgatattgc ttcgatatgg cctgttttac
     1381 tgggagcagt cgtcgtgtgt tccatcgtca cgatggttgc agcgggaaaa acggcagaag
     1441 gtgtagaagc tttgctgggc tatgtccgta agaaaaaagc agaaagggta tctgtaaacg
     1501 aaatggagga gaatcaaatt gactgagttt tttgtgaatc cattatttgg cttaagtttg
     1561 actttaggag tgtacgtatt atctgacaaa tttttgcgga aatataaact gcctgttttg
     1621 aatcctctcg tattatcgat tgttgttatt atcttatttt tgcatttttc tgggatcagc
     1681 tataaagact ataatatcgg gggcaatttt ttgactatgc tgattactcc tgcgacagtg
     1741 gcacttgcca tccctcttta taaaaacttc cgtttactga aagagaattt ttatcctgta
     1801 atggctgcga ttttagcagg aatagtcgca aattgcttgg ttagtgtagg gttcggttac
     1861 ttcttctctc ttcataaaga gatggtgatc tcattactac ctaaatctgt aacgacggct
     1921 atttctgtcg atttatctca tacgatgggc ggggtgaatg ccgtgacact tgctattgtt
     1981 gtctctacag gtatttttgg ttcgctgatc gccactcata ttttccgcct attcaagatc
     2041 gaaagtccag ttgctcgcgg tgtggcgtta ggaagtactt ctcatgctat tggtacagca
     2101 aaagcaatcg agattggtga aatagaagga atcatttccg gacttgcgat atgcgtcaac
     2161 ggaatattga ccgttttatt gctaccgtta ttctttcagc cttttgctgg cttgttttga
     2221 tgcgaaaacc actattttat gctacaataa ccaaaacgtt cgagcataaa aaaggaggga
     2281 aagacgtgta tttgtcttcc gagtatctta aacaatttga taaagatcta tattataaga
     2341 ttctcctcct agaaagtttt gaggatcaag cttggcatac cgctgcacaa ttagcgcaag
     2401 ttgttcagct ggatgcccgc agcgtgtcga aatacctgaa cgagctgtct aaaaactatc
     2461 agcagtttag cggtaagaca caccctttgt ttacaaaaaa tcaccgttct ggttataact
     2521 tttatgatac gctcgattca attgaacatg agcgtttttt gatctatctc gtgcaaagta
     2581 cgctgaaatt ccagttgctg cacgacattt tttttgaaga attccataca atgtaccaat
     2641 ttgcgcaaaa gcattacatt agtgaatcta ctgctcatcg aaaaatcaat gaatggaaac
     2701 agcaactgca aacttacgga attcggctgc aacgaggtac atatatcgct caaggcgaag
     2761 aagaaatcat tcgactttac ttgcatatga cattctggca gttgtttcga gggaaaatct
     2821 ggccatttga aacgatttct caaatggacg taaagaatat ggcagaacat atcatggctt
     2881 tttttaatgt tcgtctgaac gagatcaaaa aaagacgatt ggaatatatg ctaggtgcct
     2941 tttttttaag aaaatcacag aagcattatg ttgtattgaa tgagaaaaaa agacggctga
     3001 tttctgacaa tctgttgttc cagcgatttt gccaggtcat ggagccggta tttcctaatt
     3061 attttcaagt agaagacgag ttaggcgcat tatttcttgt actgatgact cgtgaagaat
     3121 attatagtga tcctaagata cggaagaaaa tttttgattt tcatcaacaa gcaaaaacac
     3181 cgccctttac agccctttca gaagcaaaag ccgctctttc tctttatcag gaagagcagg
     3241 ggctgccagc agaaaatcta accttcgagg cagaaaacta tttattcagc agtcattttt
     3301 tcgcctatct ttttcctaat gcgaaagaaa cgattgatgg caatagtagt gattttatta
     3361 atcatttagt tattgaaaac aaggaattga aacaatggct cgtacatttt tttgaaagta
     3421 gacacaaaca tcccaatcat ctggcattta aaaatcatgc gtttttgatg gggcggtatc
     3481 tgactgtgtt taaaacatta ggagccttta ctccacagct tccaaagatc acgatccttt
     3541 taatgacaga tttcccatta tttgaagagc aactgttaga ggagggatta cggacatttt
     3601 tccgtaatga gtatcagctg atttttctgc caacagatta tcgggggaga gaagttgatc
     3661 ttctgatttc gacaagtaaa gtccaccgca aaccatgggc ggatcttgac tattttattg
     3721 tgactgaaga actaaaatta attgattaca tccaactatc tcaaaaattt gaaatgattc
     3781 aaaaacaaaa actattaaaa caataacaaa gaaggagaaa aaagatgaca tttgaagaaa
     3841 ttttaccaga attgaaaaaa ggtgcaaaga ttattcgtaa aggatggagt ggatttgaac
     3901 tttatgttgt tctcgtatcc gatgatgaat ttgatggctg tccggtgacg ccgtacttat
     3961 tgatcaaaac aagtgacgaa ggtttttcca gcttcgctcc tacagtttgt gatattttag
     4021 cagatgattg ggcgatcgta gaatgaaaaa ccagtatcca gatctagcta aacaggttgt
     4081 atttatcaca ggagctgctt ctggaattgg tgctgcgcaa gcccgagcgt ttcttgaaca
     4141 agatgctttt gtatttggat tagatcaaaa gttcggtaaa atggaagaaa tttctttgcg
     4201 atttcctgaa acttttgcct acgcactagg tgatgttcga caaatgaaag aactccaaca
     4261 agcggtatcg aaatgccaaa atcattttgg tgaagttacg attttactga atacagcggg
     4321 gatattggat gcctacaaac ccttgatgga aactgacgaa tcgctgtggg atctgattta
     4381 tgagacgaat gtgaaaagca tgtatcagct gacaaaaatc atccttcctg atatgatccg
     4441 ccaaaaaagc ggaacgatca tcaatatggc ttcgattgct ggcttgattg ctggtggagg
     4501 tgggatcgct tatacaagcg cgaaacatgc gattgtcgga tttaccaaac aactcgctct
     4561 ggattacgca ggacagggta tccgagtaaa aggaattgct ccaggagcca tccaaacacc
     4621 aatgaatgcg gctgattttg aagggaaagg tgagatggca gagtgggtgg caaatgaaac
     4681 accagtcaag cgatgggcac agccggaaga agtagctgag ctgacgttat ttcttgcctc
     4741 tcctcaagca agctacatac aaggaacgat catcccaatc gacggcggat ggttgttaaa
     4801 ataaccttgt gtttttttca agcttctgta tgataaaact aaagagagaa acaacggttg
     4861 atgataaaaa aagtcgatga ctgttcccga cattcgatgc cgaacagcca gttcaaaaaa
     4921 ttctttttaa taatcgttaa tattcgaaac taagcggctt ttcacgacct cttgatataa
     4981 acgatattca aaagcttgca actgcggtaa caagtccagc caaatgaaaa atataaggag
     5041 ctatttttat ttgaccattc ttattacttg ttgcagggca gctttttcac taccttctgc
     5101 gacacttcag ttggaatttt cttcccatcc tttggtcgcg gtcattacca agataaaatt
     5161 atcttgcaaa ggagaaatag aacatggaaa aaaataatac ccgttcagcc aaacgctgga
     5221 caacgacaga tgtgactcgg atggcgctag tcactggttt atatgtggca gtgacgctgg
     5281 ttttgtctgt ctttagcttt ggagtgattc agattcgtct ctcggagatg ttcaactatc
     5341 tgtccttgta taacaaaaga tacatcgtcg cggtaacagc gggagtagct ttggctaata
     5401 ttgcttctcc tcttggactg atagatgttg cagtaggaag cgtcagcacg ctgcttgtat
     5461 tgcttatcaa ctataaaatc actagtcgca tcaaaaacat gaaaatcaaa atggccgtga
     5521 cagcatgcgt atttgctttt tccatgttta cagtagctgg tcagctaacg attttatatc
     5581 aactaccttt tttcttgaat tggtgggtga ttgctttagg agaattggca tcaatgattg
     5641 tcggtgggat aattatttat tggatcggta aaaaagtgga tttgtcaaaa taaagatctg
     5701 tttaatttaa aatagggcag catggaagag actgaatgtt gagtcttctt ccatgctgct
     5761 tttttgatta atttgtatga agaaatcgat ttagccgttt tttcatcaag ataccgatat
     5821 aagctgctcc agttgccttg attgcttccg gcagtaaaaa cggcagtagt ccactggcaa
     5881 atgcagttgt aaaagtaaga tcaccactta ttttcagcca gatcgttccg cagatcaatg
     5941 cagcaagaga gcccattaga ttggcaaaga ttgcatataa gtaatgagca tccgttcttt
     6001 cgatcatcca accagttatc cacgtttgta agagaaatcc aatcaaatat ccgccagttg
     6061 gcccaaataa aacagctata cctccagtca tccctgaaaa tacaggaaga ccaatcaagc
     6121 caagtaaaag ataaactcca acagatatca atcctagttt tcttccgaga aaagttgctg
     6181 ttaatccaat agcaaatgtt tgccctgtca aaggaaccaa accaaaagga atggtcatct
     6241 gagaaaggac agaaataatc gcggcaaatt ccgcagcaag tatcaattct ttcaatgttc
     6301 ggttcataac gccctctttt cgttaactta atatttttat ttggttaacg aaattgtaaa
     6361 acaaagtgat tactttgtaa atagaaaata taaaaaaaga ccagctatcc aatagatagc
     6421 ggtctttttc aagaaagctt ttgaaattat aagctttggt tgtagtattc aacgacaaga
     6481 gcttcgtcga tttctggata tagttcgtca cgttctggta aacgagtcaa tgaaccttcc
     6541 aatttttcag cgtcaaagct tacgaatgct gggcgtccaa cagttgcttc tacagcttct
     6601 tttactgtta cgatgttttg tgatttttca cgaactgaga tcacttgacc cacttctacg
     6661 tggtatgaag ggatatcaac gcgtttgcca tcaactaaaa tgtgaccatg gttaactaat
     6721 tgacgtgctt gacgacgagt cgttgcaagt cctaaacggt aaacaacatt gtctaaacgt
     6781 tgttctagta agatcatgaa gttaacacca tgtttacctt ctttgatttt gctagctttt
     6841 acgaacaagt tacggaattg acgttcgttc attccgtaca tgtgacgaag tttttgtttt
     6901 tcagttaatt gcatgccgta ttctgattgt ttgccacggc tattaggtcc atgttgacct
     6961 ggtgcgtaag ggcgacgtgc taattctttt cctgtgcctg acaatgagat gcctaagcga
     7021 cgagaaattt tccatgatgg tcctgtataa cgagacatta aaatttcctc caatgaatat
     7081 aagtttttgg agtaaaataa tccgttgaaa aattcataat catatagttt gttcttcaat
     7141 cttcaccttt gcagccgcgg ttacacaatc gaacccgaga cggggcaaca aactagggat
     7201 gagttattat ttttctgctg cattatttta cacaaattac agtatatgat agatcatcca
     7261 cgaatgtcaa gataattagt acataaaaac gctttacaaa cctaggctgt ctttataaaa
     7321 tgagagggta tgtgcaaatg gaaaaaagcg aaggaagttt aaggagaaaa tcaatgaatc
     7381 cggtatcacc attatttgag aaaatcgatg cttcaattga aaagaaggcg aatctttttg
     7441 aaaacagctt ttcacgttac gctgttcgag cggttctcgc ctgtttgttt ttgactttag
     7501 gaacggctgt ggcatttgcg atcgcgatga agggggaaga tatcgcgcat ggtctaggaa
     7561 agattttata cgcatttatg ttcagctggg gtctggtcat gattctatac atgaacgccg
     7621 aattaggaac atcgaatatg ctttatatga ccgtgggtgt ttttcggcag agacttgctt
     7681 taagtaaggc agccaagatt ctatttgcct gtattttttt caatatgatt ggcggtatat
     7741 tatgtggatt tctaatttct atgactacgc catttcaaga tttaacgttg gacaacttta
     7801 tgttagaatc aatagcagta aaactagcaa aaccttcagc aacgattttg gtggaaggga
     7861 tgtttgcgaa tatcgttgtc aataccgctg tactgatcag catgcggatg aaagaggatg
     7921 tcggaaaagt gatcacagtc gtattcatca ttttcatttt tgctttttta ggatatgaac
     7981 acgtgatagc gaattttcct gcctttacgc ttacttactt tgcttctcac ggacagatgg
     8041 atggaatgac agtcagcaat gtgttacata atttgttttt tgctttggca ggaaattata
     8101 ttggtggcgg tttagtaatg ggactgggat atgcttggct gaatcagtca aaatcatcct
     8161 atgtggacta aaaagtcgtt cattaaaggg gaagtgccat ggaagtttta gtttttccaa
     8221 aaaatgagga aaaagcgtta caaatggaaa agtacatgaa aaatcttttt tcctttgctg
     8281 gagtacctaa gccggaacgc gctcttttac aacaaccgtt actggatgag agcaaaaatt
     8341 tatctatttc tgaagtttta gcttttgttc attattatta tgaacaaatc gaaagagagt
     8401 atcagtatgt agcgatcgat ttagtccaga aaaacatcaa acggcttaca tttgaagact
     8461 tagtcgtatt gttgccacta gttcagaaaa aagaatggtg ggacagcatc gactcttgga
     8521 gaaaagtata cagcacatgg tgtaaagcct acccagagca gcgtgaagaa gtctttcaat
     8581 tgtttgaaaa aaaggaagat ttttggctac gtcgaatagc aatcacgttg caattaggtt
     8641 ataaacagca aacagacaca ggactattga tccgagcaat agacgaagat cgtcaaacaa
     8701 aggaattctt tatccaaaaa gcaattggtt gggcattacg cgattatagt aagaccgatg
     8761 ccgattgggt aaaaagacag ctgacaacag atttgagtaa gttagctgtt cgagaggcaa
     8821 gtaagtattt ataagaaagc gagacgacaa gagatgagac gcaaattatt tgcttttgat
     8881 attgatggaa cgttgttaga ttcaaacaaa caagctttag atagcacaag agaagcattg
     8941 gaaaaattac gcaaagccgg tcatctggtc acgattgcta cagggcggag ccgatttcat
     9001 gcgcaagaga tcattcgcga tcttgatttt acgaactaca ttttatgtaa tggagcggcc
     9061 ggttttttag accatgaaca agtatataag aatttactag atcgcgacca gttggatgcg
     9121 ttcgttcaag aagcgcatca tgagcagatc gatacagctt ttgtcagctt agacaatatc
     9181 aagcgttctt cttccaatga tgtaacggtg atggaagaag cgatgcattc tttcggtgct
     9241 gtcttgcctg aattagatca gttttttgca gaggaacaag aaatatacca agcgttagca
     9301 tttttcgatc aaacatatga tgggaagttc tcttcatatg ataaaattcg atttgtccgc
     9361 tggcatgaga acagtgtaga tgtcgtacct aaaaatggtt ctaaagcagc gacaattctg
     9421 cacttagcag aacaagtagg catccctcat gaagacatca tcagctttgg cgatggacaa
     9481 aacgaccgtg aaatgttgcg catgtcagga attgggatag ctatggggaa tgctgtgcca
     9541 gaagtacaag cagaagctaa gatggtcact gatacaaacg atcaggatgg aatttggaag
     9601 gcattgaaag agttaagcgt gatttaaaag aggttgtgaa aaagctgttc tgcatcaagt
     9661 aataagccca gccaaacaaa aatagctcct cctatttttc gtttttctgg gcttattacc
     9721 gcagatgcaa gctttcgaac accgtttaag gaagaggtcg tgacagaagt ggtcagtttc
     9781 aagaaataag aagggattta cgaaaattgc ttctcaaatt tttgtaaaat tacggcttat
     9841 ttctgaagaa gtagatgaaa caacgtttat tcgggaaatg ggactgtgac aagatgtttg
     9901 tcatagtccc atttttttat tttatttttc aaaactaaga aataccagga aagagttcct
     9961 tttgtttgac cttttctttc ttattgaaaa caactataat atagagtgat gttaaaagag
    10021 aagaggaatt tagtgtgcgg gtattgttta ttggtgatgt agtaggttca atgggacgag
    10081 agatgattac tgaatatctt cctagattga agaagaagta ccgtccgcaa gtaacgattg
    10141 taaatggtga gaatgccgct tctggacgtg gaatcacaga aaaaatctat aaaaaatttt
    10201 tgcaagacgg cgtagatgtc gtgacgatgg ggaatcatac gtgggacaac cgcggaatct
    10261 ttgagtttgt aaatgaagct aaaaagatgg ttcgtccggc aaattttccg gaagggacgc
    10321 ctggacaagg gatggtcttt gtcaaagtga accaagtcga actggcagtg atcaacatgc
    10381 aggctcgttc ttttatggtg gacttggatg atccttttcg taaaatgaat gagctggtag
    10441 aagaagccag aaaacgtacg ccgatcattt ttgtagattt tcatggagaa acaacaagtg
    10501 aaaaacaagc aatgggctgg tttttagatg ggaaagtttc agcagtggtc ggtacacata
    10561 cacacgttca gacaaatgat gcgcgaattt tgccaagagg aacagcgtat ctgacagacg
    10621 ttgggatgac aggtccctat gatggtattt tgggtatgcg cagagaacct gtaatcgaaa
    10681 agtttctgac tgcattacca aaacgttttg aagttgtgga acaaggccga tcgattttaa
    10741 gcggctgtat cttggaattg gatgatacga caggtcatgc aaaagaaatc cagctgattc
    10801 agatcaacga tgatcgaccg ttcatggagt agaaagagag gcagtttttt gcagcaggag
    10861 ccggaaattc aaaaagaagt aaggaaactg acaaaacttt taagagagaa tgaaacgatc
    10921 attcgctaca aagaacttga agaaaagatc cagcaaaacc agtatttagc tgagttgaga
    10981 gaaaaaatca aacaagcgca aaaagatgct gttcattttg cccactatga caaaccagca
    11041 gcagaaaaag aagcaatcaa acaagcggat caattcatgc aagaatttga tcagcatccg
    11101 cttgttgtag cttatagaaa acagctgttg gaagcagatg atctgcttca tcatttaaca
    11161 actatgatac aagaagaaat caatggacaa atagaggagg aaaaacatgc ctcaaaaaac
    11221 taaaaatact cctatgatgg aacaatatct aagcatcaag gcccaatacc aagatgcttt
    11281 tttgttctat cgtttaggag acttttatga actattttac gaagatgcga tcaaagcgtc
    11341 acagcttttg gaattaacgt tgacgagcag aaatcgaaat gcagaagaac ctattcctat
    11401 gtgcggtgta cctcaccatg cagcacaagg atatatcgat gccttgattg aaaaaggcta
    11461 taaagtagcg atttgcgagc aggtagaaga tccaaagacg actaaaggga tggtcaagcg
    11521 cgaagtgatc cagctagtca ctcctggtac agtcatggac agcaaaggat tgtcggcaaa
    11581 agacaacaat tttttaacag ctgtagttag tcaaggaaat gaatttggct ttgcttatgc
    11641 cgatttaagt acgggagaat tgaagacggc tcggttgcat gatgaagaag ctgttttaaa
    11701 tgaagcctct gcattgcaga caaaagaact cgtattaggc agtgagatca ctgataccct
    11761 tcgtgaacag ctaagcgggc gtttagggct agtcttttct caacaaaatg agatggaaga
    11821 aaacgcggaa tttagctttt tgacaagcga actagtcgat ccgctggaaa aagaagcaac
    11881 cggcaaacta ttgacttatt tatcggtgac acaaaagcgg agtttagctc atatccaaaa
    11941 agcagtagaa taccaaccag atcatttttt gaaaatggat tattattcaa aattcaacct
    12001 agaattaagc caatctattc ggactgggaa aaagcacggc actcttctat ggttgttaga
    12061 tgaaacaaaa acagcaatgg gtggccgttt actaaaacaa tggctagacc gtccgcttat
    12121 ccaggcaaaa caaattaaag caagacaaga aatggtcgct tctttgctag acagctattt
    12181 tgaacgtatc gatttgcagt cagcattaac gaaagtctat gatctggaac gcctagcagg
    12241 tcgagtagct ttcggtaatg tcaatgggcg agacttgatc cagctgaaaa cttctttaga
    12301 acaagtgcca atgatcagag ggttgattga aggaatcgat caaggtcaat ggcaaagtct
    12361 gctttcagaa atgcagccga tggaccatct cgtccaattg atcgaccaag ctatccaaga
    12421 tgatccgccg ttacaaataa cggaaggaaa catcatcaaa gatggattca atgagcagct
    12481 cgatatgtac cgttctgcta tgagaaacgg aaagaaatgg ttggcagaac ttgaagcaaa
    12541 agagcgtcaa gaaacaggaa tcaagaactt aaaaatcgga tttaaccgcg ttttcggtta
    12601 ttatatagaa attacaaaag caaatcttgg aaatgcagaa ctggagaagt atgaacgaaa
    12661 acaaacttta gcaaatgcag agcgtttcat cacaccagaa ctaaaagaat tggagacaca
    12721 aatcctcgaa gcagaagaaa aatctgttga tttagaatat cagttgtttt tagctgtgag
    12781 agaagaagtc aaaaaggcta tccagccgct acaagtactt gcaaaagcaa tcagtgcagt
    12841 agatgtgcta caaagttttg caacgatcag cgaacgctat cagtatgttc ggccagaact
    12901 tgtcagtgac aagcatcagc ttctgatcaa agacggacga catcctgtag tagaaaaagt
    12961 attaggacat caggaatata tccctaacag cgtagaaatg gcagaagacg aaatgatctt
    13021 gctgattaca ggaccgaata tgtctggtaa aagtacgtat atgcgacagt tggcattaac
    13081 tgttctgatg gcgcaaatgg gctgtttcgt tccggcaaaa caagcagttt tacctatttt
    13141 tgatcggatt tttacccgaa ttggtgcaag tgacgactta attgctggac aaagtacttt
    13201 tatggtagag atgatggaag ccaatcaagc cctgcgttat gctacgccaa acagtttgat
    13261 tttgtttgac gaactagggc gaggaacagc aacctatgac ggaatggcgt tggcccaagc
    13321 aatcatcgaa tatatccatc gtcatgtaca agcaaaaaca ctcttttcta ctcattatca
    13381 tgaactgacg gtacttgaga aagaacttcc gcagcttaaa aatgtccatg ttggagccgt
    13441 tgaaaaagac ggagaagttg tcttcttgca taaaatgatg gatggacctg cggataaaag
    13501 ctacggcata catgtggcaa agatcgcagg attgccttcc aatctactag aacgagcagc
    13561 agatatcctg catacgctag aaaatggcga taacggacac catactgttc cagctgaagt
    13621 agaagagaaa actaaaacag ccaaacagga gcaaataaaa ccacctgcag aaaaagtaaa
    13681 tcaagaccat atacaagacc gtgtaaaaga agaaaaccaa cagctttctt tattcagtga
    13741 attatccgaa aatgaaacag aagtcattga atcactgaag caaataaatt tactagaaat
    13801 gacgccaatg gatgcactaa acacattata tgaattacaa aaacaactgt aggaagaagg
    13861 cggatatcga tggccaaaat ccaagaatta tctgaacgtt tagctaatca gatcgctgcg
    13921 ggagaagtag tggagcgacc agcttccgtt gtgaaagaat tagtggaaaa tgcaattgac
    13981 gccggcagta ctcagattga tatcttagtt gaagaagcgg gactgcggac gatccaagtg
    14041 attgataacg gagaaggcat tttggcagac gacgtagaga atgccttcaa gcggcatgcg
    14101 acaagtaaga tccataatcg cgacgatctt tttcgcatac gaacgctggg ctttcgaggg
    14161 gaagctttgc caagtattgc ctctgtttct gaaatgactc tggaaacggc gaatcaggaa
    14221 gaaaaacaag gcacctatct gtcgctgaaa ggcggagaag ttattgagca tcgaccagca
    14281 ccactgcgtc aaggaacgaa gattaccgtc agcaatctgt ttttcaatac gccagcccgc
    14341 ttgaaatacg tgaaaacact gcacacagaa ttagcgaaca tcggggacat cgtcaatcga
    14401 ctggcattga gtcatccatc gattgctttt cgtttagtac atgatggaaa caaaatgctg
    14461 gcaactgctg gaaatggcga tctgaaacaa acaattgcgg gaatctacgg attatctacc
    14521 gcaaaaaaaa tgctgaaaat cgaaggaaaa gatctagatt ttgagttatt tggctatgtt
    14581 tcattgcctg aggtgactcg tgccagtagg aattacttat cgacgatcat caatggacgt
    14641 tttatcaaga acttttcctt gaataaagca atcgttgcag gttatgggtc aaaattaatg
    14701 gtcggtcgtt tccctattgc tgtattggaa atcaaaatgg acccgctttt agtagatgta
    14761 aatgttcatc cgacgaagca ggaagtccgg ctgtcgaagg aaaaagaact aacagagcta
    14821 attagtaacg ccattcaaaa agcattgtct caagaaaatc tgattccaag tgcagcggat
    14881 aatctacggt ttaagaaaaa agtacctgat cagcctaaaa cagaacaaat cgaaatcgat
    14941 ttgtcctacg cttttgaaga agaaaaacct aaaaagccaa gtagtttgaa ttttgatcga
    15001 gagacaggta cctttttcgt agaagaacta aagaacacat ttgttgacag aattgttgat
    15061 aagtctgtgg aaaatgttga aaactattct ggtttttctg aagaaaacac cacttatcca
    15121 gaaaaaacag cgaaaacggt ggataagttt ggtgaaaatg ttgataactt tgtagaaaaa
    15181 acagtggaaa aatctgaaaa gaaagaattt cccattgtgg ataacatgga gaaaacaaat
    15241 gaagaacaag cagataaatc cgtagaagag gatgaaaaga tttacgagga agaattgaca
    15301 ctccatcctg aatttgattt cactcaagga aacgacaaaa cattagaaca agccatcaag
    15361 aaattagaaa cggaaaaacc agtacaacgt tttcctcagt tggaatattt tggacagatg
    15421 cacggaacgt atctttttgc ccaaagtagc gatgggttgt acatcattga tcagcatgct
    15481 gcccaagagc gcattaagta tgaatatttc cgggaaaaaa ttggggaagt atctgatgat
    15541 ctgcaagaat tgcttgttcc atttgtgtta gactatccaa acagtgatgc tttgaaatta
    15601 aaagagcaaa aacacttgct agaagatgca gggatttatt tagaagaatt tggtcaaaac
    15661 agctttattg tacgggccca tcctacttgg tatcccaatg gagaagaaga atcgattatt
    15721 cgggagatga tcgatatgct gctgacaact ggaagtgtga gtgtaaaaaa attccgtgaa
    15781 gcaacagcaa tcatgatgag ttgcaaacga tcgattaaag ccaaccatta tttgaatgaa
    15841 gcacaagcac gtgtgctgct gaaggatctg gcacgatgcg aaaatccttt caattgtccc
    15901 catggccgtc cagtattgat tcactttacc aattcggata tggaaaggat gttcaaacga
    15961 atccaagacc cgcattaagt caaagagaag agggaaaaca tgaagattgt attagcatcc
    16021 caatcaccaa gaagaaaaga gttattggct catatcgttt cagaattcga gattgaacct
    16081 gctgatatcg atgaaacgcc atttccagac gaacagcccg tcgattatgt tcgtagaatg
    16141 gctgaagaaa aagcacgaac tgtctgggaa caagatgaaa aagaagacca tttagtgatt
    16201 gccagtgaca cgactgttgt tctcaatcaa gagatcatgg gtaaaccaga agatctggct
    16261 gaggctgaat tcatgctgaa aaaactaagc ggggaaacac atactgtata tacagctgtt
    16321 gttttaaaaa caaaggaacg agaagaacgg atactagcag aagcacatgt cactttttat
    16381 cctttgacag acgaagagat caaacgctac ttggagacgg gggattatgc ggataaggca
    16441 ggggcatacg gcatccaaaa tgctgcgagt gtattcgtca aagaaatttc tggagattat
    16501 tacagtatcg taggatttcc gattggtgct gtcaatcagg cactgaaaac attcatttaa
    16561 acaaaaaagc ggactatctg attttcgatt gaaaatccat gatagcccgt tttttttatt
    16621 gaaaagatta cttatctact ctgactaaga ttttacttca gctttaccga ctgcttcgcc
    16681 cgggcgaaca agtcctgatt ggtcaagaga aagtcctgca actttttcat tattggtcaa
    16741 agcaacaata atgtcagttt tcttgccagc ttgttctatt tctggcaaat ccatttcagc
    16801 aatcagggtc tctggtgtga caaggtatcc ttctttcacg aatacattga aaggaacacc
    16861 ctttagttca acagtatcta atcccatgtg aacaagtact tccagcccct cattagaaag
    16921 aataccaatg gcatgttttg tttcaaagac actcgtcact ttaccgttta ctggtgcata
    16981 gactttgccg tttgacggtt cgacagcata accgtcaccc atcatttttt gtgagaaaac
    17041 tgggtcattt acttcagtta tttccttgat ggtccctgca gcgactgcga ataatgtttc
    17101 ttttgttgca actgccgtac cagttgctga aacaggttcg aaagactgtg gcgttgctgt
    17161 tgcgccattt gctgtttgaa gttgtacatc agcagcattt tcaggaacag caggatcaat
    17221 cttattcaaa attccctgac gtctaaagat gatcgtcaag ccaaatggaa tgatgacagc
    17281 aatgatcatt gcgatgatga aaggtaccca tgtttctgct tggatagcta gtattcctgg
    17341 caaaccgcct actccgatcg cgttagctcg aacacccatc aagttagcaa acatgccagc
    17401 taatccagag ccgaccattg cagcgacgaa aggatagacg tatttcaaat tgattccgaa
    17461 catagcaggt tcagttacgc ctaagtaaca agaaatcatt gctggaatcg agatttgttc
    17521 ttctttttta ttcccacgat gtaagaagat gatggctaag actgcagagc cttgggcgat
    17581 atttgataaa gcaatcatcg gccataaatt cgttgatccg aaatctgcaa tcagctgcat
    17641 atcgatggca ttggtcatat ggtggagacc agtaatcact aaaggagcat acaggaatcc
    17701 gaatacggca ctaaacaacc agctgataga agacgtcaat ccagtattta cgatatttga
    17761 gatccagctt ccgactgtcc agccgatagg acctaaaata acgtgtgcag ctaaaacagt
    17821 tggtaacaat gaaaacaatg gaacaaaaat catcgagatc gactgaggga tatatttgcg
    17881 gaagaagatt tctagatacg ctaacataaa ccctgccagc atggctggaa taacttgcgc
    17941 ttgataacca atcatctgga cttgagcaaa accaaaatcc caaaatggaa tatctgctgc
    18001 ggctgtggaa gctacactat aagcatttaa taattgcgga gaaactaacg taattcctaa
    18061 aacgattcct aatatttgtg tagtacccat cttttttgca atactccaag tgatacctac
    18121 tggaagaaaa tggaaaattg cttcacccgg taaccacaaa aaggcattga caccattcca
    18181 aaacttcgag tgttcaacga ttgttccacc aagactttca aactggatac cttcaagtac
    18241 atttctgaat ccaaggatca atccaccgac aatgattgca ggaatcagtg gtgtaaagat
    18301 ttctgctaaa acggcaatag cacgttgaac gggatgaagg ttttgttttg cagctgcttt
    18361 tccttgttct ttggaaaccc cttcaactcc agatactgca acgaagtcgt tataaaaagt
    18421 agaaacatcg tttcctatga tgacttggaa ctgtcctgca ttcgtgaaca tcccttttac
    18481 gctaggtata tcttcgatcg ctttttcgtc cgcttttcct ggatcattca ggacaaaacg
    18541 catacgagtc gcacaatgac taactgcagc aatattttct tttccgccga tatcatgtaa
    18601 cagcttctcg gcatctgctt gatattttgc catatcaata aacccctttc tcttaacttg
    18661 tctatgcaag ttgtttcttc atttactata ctttttttcg gagagaattg caagcgtttt
    18721 attaaaaagt acgagagaaa atgatatcgt tttaaaaagc ttaaaataag cttttcttaa
    18781 aaataggaac attgaaatac aaaaagagct gtagtatagt gaagaaatga tggaagaacg
    18841 agtgagggaa aaaacatgaa taagttcaat gaaatttttt tagatttgga acagaaaatt
    18901 ttagcgaaag aatatcctcc gcattcgctt ttacccagtg agaaccaatt gatcaaaatt
    18961 tatggtgttt cacgagaaac aattagaaaa gcgctgaatc ttttgactac ggaaggttat
    19021 attcaaaaaa aacaaggaaa aggttcgatc gtactggatc ggaatcgctt cgactttcct
    19081 atttctggat tgactagcta taaagagcta caggagaccc aacgtattcc aagtgaaacg
    19141 atcatccata cgttagaaga aacagaagtg acaaaagcat tgaacgagat cactggctgg
    19201 gagatcggtg cgcctgtttg gcatttgata cgcgaaagaa aaatcaatgg cgaagtagtt
    19261 atattagaca ccgattatct attaaaggaa atcgttccta ttttaactcc agcacaagca
    19321 caaggatcga tctatgaata ttttgaaaat gagctttctt tgacgattgc ttacgcgcaa
    19381 aaggagatca cagtagaaga agtaacggaa atcatgaaaa cagcgatgga tcttcacgat
    19441 gacacttatg cagtagtagt cagaagcctc gttcatctgg aagatacccg ctgtttcgag
    19501 tattctgagt cggttcaccg attagataaa ttccgtttcg ttgagtttgc ccgacgccga
    19561 aaagtgtaaa tagttcaaaa gaaagcaaat tgttggcatc tgtccacttt ggcgctatga
    19621 taggatcaca aagggggcat ttttaatgga taaaccaata agaccagaaa aagaagaact
    19681 gaaagaaaag ttatcaccca tggcttatgc agtcacacaa gaaaatgcaa cagaacgacc
    19741 atttagcggg aaatatgatg atttttacga aaaggggatc tatgtagata tcgtcagcgg
    19801 tgaaccgtta ttcagctctg cagaaaaata tgatgcaggt tgtggctggc catcattcag
    19861 caaaccaata acgaaaagac aagtgcgcga aaaagccgat ttttctcatg gcatgcatcg
    19921 aatagaagtt cgaagcaaag aagcagattc tcatctcgga catgtgttta ccgatggacc
    19981 agtagatcaa ggtggtctac gttactgcat caattctgcc tcactgaaat tcattccata
    20041 tgaaaagatg gatgaattag gttatgggga attcaaatca cttgtagaat aatggaaata
    20101 cacaaagagc ggtacaatac ggggatttac tcgatcgtcc gctctttttt tattcaagcg
    20161 attgctattt gaaagaaacg ctccctagac tccttttttc acaaggtgaa atgtgctaca
    20221 ataaagggaa cgtatgtgtg caaaagaaag aggactagaa tatgtatgaa tatctgacag
    20281 gtgtggttac ctttatcaac ccctattaca tagtagtaga aaccaatggt atcggctacc
    20341 agatcgcact aggaaatcct taccgctaca gtagcaaact aaatcaaaaa ataaaaattt
    20401 atgtccatca ggtaatacgt gaagatgcgc atcttctgta tggatttgat tcgctagaag
    20461 aaaagcaact gtttttgcga ttagtaagtg tttcaggaat cggtccgaaa agtgcccttg
    20521 caatcatggc aagcgaagac cactccggtt tgattcaagc agtagaatcc agtgatgtca
    20581 cttatttgac gaaatttcca ggagtaggga aaaagacagc acaacaaatg atcttggatc
    20641 taaaaggaaa attcggtgag ctgagtatcg atacaccttt tagtctattt gatgaagcaa
    20701 acacaaaaga cgctacagct ctatctgaag caatagaagc tctctcggct cttggttaca
    20761 gtgatcgaga aatcaaacgt gtagaaaaac aattaaaaga agtagataat cagacaacgg
    20821 atgagtatct acgccaagca ctcaagctaa tgatgaaaaa ataaggagga gaccaaatga
    20881 cagatgaata cttgttatca ccagaatctg gcgaaaatga actgagtttg gaaaaatctc
    20941 ttcgaccaca gctgctttct caatatatcg gacagagaaa agtcaaacaa gaacttcaga
    21001 tttacatcga agctgccaga aatcgggaag aacctttaga tcatgttctg ttgtacgggc
    21061 ctcctggatt aggaaaaacg acgatggcca tggtcattgc taatgagatg accgtaaata
    21121 ttcgtacgac gagtggccct gctatcgaac gtccgggtga cttagtagca atcttgaatg
    21181 aactggaagc aggggatgtc ctgtttatcg atgagatcca tcgattacct agagtggtag
    21241 aagagatgct ttattcagct atggaagatt tctatgtaga tatcatggta ggacaagggc
    21301 caaccgcaca tccggttcat ttccctcttc ctccctttac tttgattggc gcgacgacac
    21361 gggcaggtat gctttctgca cctttacgag atcgatttgg catcatttct catatggaat
    21421 attatgacga acaggatctg caggagatcg ttttacgttc ttcggatatt tttcaaacag
    21481 agatcattga agaaggggca ttggaaattg ctcgccgatc acgaggaacg cctagaatcg
    21541 ctaatcgctt gctcaaacgg gttcgtgatt ttgcgcaagt ccaaggagac gggaaaatcg
    21601 atcgtcctat agcggatcag gcacttactt tgctacaggt cgatcaggct ggattggatt
    21661 atgttgacca gaaattgttg cgaacaatga tcgagttata tggcggcgga ccagtagggt
    21721 taagtacgct ttcagtaaac ataggagaag agcgtgagac ggtagaagat atgtatgaac
    21781 cgtatctgat ccaaaaagga tttttaaagc gtacacctag agggcgaatt gctactgctt
    21841 atgcctatga acattttggt tacgactatc atatatgatt tttgctttta gattggagga
    21901 aaatgaatga gtgatacaac acgtacgaaa caattgatca aaaacgtatt gattgatttg
    21961 tgccaaacgc aatcttttcg aaaaatcagc gtccaaaaaa tcgcaaacga agcaaaaatc
    22021 aatcgacaaa cattttatta tcattttaca gataaatacg atttgcttcg ttgggtctat
    22081 tgtgaggatg cattgcatta tttagatcca caatgtcttt cacttgataa ttgggaagag
    22141 caagcattga aaatgctgaa agcaattgct gagaagaaag aattttatgc aaatactgtg
    22201 ctttctgacc cagaaatttt acaaaaagag ttttctcaac tgatccaaaa atcatttatc
    22261 aagatttttg aacaaatgga tgaagaaaga caactttctg caagagacaa actattttat
    22321 gctcgttttt tttcttacgg ttgcagtggt gtattagtca attggatcac gaatgaatat
    22381 gaagaaacac cgatggaaat cgctatgcag cttttccgtt tagccaagga tacggaatta
    22441 tattcttatc gtctttatgc aaaagaagag ttggaagaac agtaatagaa aaattttttg
    22501 ttggaaaagc aataatcgct aaaaggagat ggggaaatga caaaaatttt atttgtttgt
    22561 ttgggaaata tttgccgttc accgatggca gaaggcttgc tgagaaaacg aatagcatta
    22621 gaaggaaggg aagcagaatt ttttgtggat tctgctgcta caagcacgta tgaagttgga
    22681 aagacaccgc accctggaac gaaaaaaata ttaaaccaag aaaaagtaga tatgacgaat
    22741 atggttgctc ggcaaatcac accccacgat tttgaaactt ttgattggat cattggcatg
    22801 gatcaagaaa atgtagaaga actgaaacga agagcaccac gctcagcgca ggggaaaatc
    22861 catttgtttt taagtagcat tcctggaaaa gaaaaagaaa atgttcccga cccatactat
    22921 accaataact ttgatgagac atatcacttg atcacagaag gcctgaatca ttggtggaat
    22981 atttggattg atgaaaataa aataacaaat aatttgtaaa gttaacgctt tctttttaag
    23041 gcgatgttag agcatttcct tgtttttttg taaaatgtta ggtaaaataa aattaattaa
    23101 gtaacacttt cttagctaaa ggaggagtgc attcgatgaa cgaagagaca gtatcgccaa
    23161 tgaagaagct tacgaagtta gttactcata cttcgtatca actttttgat tcttttgtaa
    23221 atgacactga agaacatgct aagaatgtac gattgcaagg aaagtttatt cttgagctgc
    23281 aaaaagctgc tactgaacat agtctagtta tcttacaagt aagagcaaat cgtttgagta
    23341 gcaagtatga aacgatctca ggctgggtgg cgacaaaaac ggtcgaaaaa gaccgagtga
    23401 tgatccgcct acaaaacgaa gagcagcaga tccggatggt tcctgtagaa caaattgaaa
    23461 aaataacaat cctttcacca gcaggggaac aggaacggtt atcaaaataa tttataccag
    23521 atagaaaagc acgtatgagc ttgtaccatc aatcattact aatcaaaaat aaccgaatgc
    23581 tttaagctga tatcagtaaa acggaacgtt ttaggatgag tatcatagca tgacagcatt
    23641 cggttgatta gttgaatcga ttggatggta caagtttttt tgataaaaaa aggtcctgct
    23701 agccggggag ctagcaggat aaggagttaa aaatgaaaaa gtgttgttag ggttgtttgt
    23761 tggtatgctt atataatagt cattagttgt gaatattttg tgacgaaagt tttcgaaagt
    23821 tgttaagttt ttgagagtat aaaatatttt gtgtaaatga aaaaatccat acaaaaaagg
    23881 aagtcccttc tgtagaataa agttaacgac aaccaattca cagaaaagag gacttcccta
    23941 tgaatgattt tactacagaa attgtgcaaa ctctagtcac taaaggcgat ttaaatgaat
    24001 tattccgttc gcacttagaa aaagcgataa acacactcct acggactgaa ttaacggctt
    24061 ttttagatta cgaaaaatat gatcgcactg gttttaattc aggtaattcg agaaacggtt
    24121 cttactttcg atcaatcaaa accgaatatg gtgaattaac attggaaata cctagagatc
    24181 gtaatggtga gtttaaacaa caaactttac cagcctacaa aagaacaaac gatacattgg
    24241 aaaccactat tatccattta
//
LOCUS       DAYYCY010000023        23137 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000023 DAYYCY010000000
VERSION     DAYYCY010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23137
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.023"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            345..569
                     /gene="nrdH"
                     /locus_tag="ACHAZB_000777"
     CDS             345..569
                     /gene="nrdH"
                     /locus_tag="ACHAZB_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFM8613470.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            728..2887
                     /gene="nrdE"
                     /locus_tag="ACHAZB_000778"
     CDS             728..2887
                     /gene="nrdE"
                     /locus_tag="ACHAZB_000778"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFM8613471.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            2937..3902
                     /gene="nrdF"
                     /locus_tag="ACHAZB_000779"
     CDS             2937..3902
                     /gene="nrdF"
                     /locus_tag="ACHAZB_000779"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFM8613472.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(3991..5130)
                     /locus_tag="ACHAZB_000780"
     CDS             complement(3991..5130)
                     /locus_tag="ACHAZB_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM8613473.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(5439..6152)
                     /locus_tag="ACHAZB_000781"
     CDS             complement(5439..6152)
                     /locus_tag="ACHAZB_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFM8613474.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(6406..7107)
                     /gene="nagB"
                     /locus_tag="ACHAZB_000782"
     CDS             complement(6406..7107)
                     /gene="nagB"
                     /locus_tag="ACHAZB_000782"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFM8613475.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            7341..8873
                     /locus_tag="ACHAZB_000783"
     CDS             7341..8873
                     /locus_tag="ACHAZB_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFM8613476.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(9064..10266)
                     /locus_tag="ACHAZB_000784"
     CDS             complement(9064..10266)
                     /locus_tag="ACHAZB_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8613477.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(10348..11037)
                     /locus_tag="ACHAZB_000785"
     CDS             complement(10348..11037)
                     /locus_tag="ACHAZB_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFM8613478.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(11143..11511)
                     /gene="rplL"
                     /locus_tag="ACHAZB_000786"
     CDS             complement(11143..11511)
                     /gene="rplL"
                     /locus_tag="ACHAZB_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFM8613479.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(11564..12067)
                     /gene="rplJ"
                     /locus_tag="ACHAZB_000787"
     CDS             complement(11564..12067)
                     /gene="rplJ"
                     /locus_tag="ACHAZB_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFM8613480.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(12271..12398)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(12477..13166)
                     /gene="rplA"
                     /locus_tag="ACHAZB_000788"
     CDS             complement(12477..13166)
                     /gene="rplA"
                     /locus_tag="ACHAZB_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFM8613481.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(13270..13692)
                     /gene="rplK"
                     /locus_tag="ACHAZB_000789"
     CDS             complement(13270..13692)
                     /gene="rplK"
                     /locus_tag="ACHAZB_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFM8613482.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            14035..14703
                     /gene="sdaAB"
                     /locus_tag="ACHAZB_000790"
     CDS             14035..14703
                     /gene="sdaAB"
                     /locus_tag="ACHAZB_000790"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HFM8613483.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            14716..15588
                     /gene="sdaAA"
                     /locus_tag="ACHAZB_000791"
     CDS             14716..15588
                     /gene="sdaAA"
                     /locus_tag="ACHAZB_000791"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HFM8613484.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(15679..16779)
                     /locus_tag="ACHAZB_000792"
                     /pseudo
     CDS             complement(15679..16779)
                     /locus_tag="ACHAZB_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324221.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
     gene            complement(16871..17758)
                     /locus_tag="ACHAZB_000793"
     CDS             complement(16871..17758)
                     /locus_tag="ACHAZB_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM8613485.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(17835..18380)
                     /gene="nusG"
                     /locus_tag="ACHAZB_000794"
     CDS             complement(17835..18380)
                     /gene="nusG"
                     /locus_tag="ACHAZB_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFM8613486.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(18481..18651)
                     /gene="secE"
                     /locus_tag="ACHAZB_000795"
     CDS             complement(18481..18651)
                     /gene="secE"
                     /locus_tag="ACHAZB_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFM8613487.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(18675..18827)
                     /gene="rpmG"
                     /locus_tag="ACHAZB_000796"
     CDS             complement(18675..18827)
                     /gene="rpmG"
                     /locus_tag="ACHAZB_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFM8613488.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            18989..19633
                     /gene="cbpA"
                     /locus_tag="ACHAZB_000797"
     CDS             18989..19633
                     /gene="cbpA"
                     /locus_tag="ACHAZB_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-AMP binding protein CbpA"
                     /protein_id="HFM8613489.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(19689..20192)
                     /locus_tag="ACHAZB_000798"
     CDS             complement(19689..20192)
                     /locus_tag="ACHAZB_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFM8613490.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(20205..20251)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            20789..21754
                     /locus_tag="ACHAZB_000799"
     CDS             20789..21754
                     /locus_tag="ACHAZB_000799"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFM8613491.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(21853..23025)
                     /locus_tag="ACHAZB_000800"
     CDS             complement(21853..23025)
                     /locus_tag="ACHAZB_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8613492.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(23012..>23137)
                     /locus_tag="ACHAZB_000801"
     CDS             complement(23012..>23137)
                     /locus_tag="ACHAZB_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8613493.1"
                     /translation="KLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
BASE COUNT         7237 a         4632 c         4142 g         7126 t
ORIGIN      
        1 aattttctta cgtgttacgc aaataaatga gaatcattct cacatagaat actctcggta
       61 attgagaatg tcaattatac ttgtgatatt atgagcgaat ctttagtatt gatagataaa
      121 aatgagaaaa aaatatttct tcatagtttt ccacaatttt ttctctatta aatttgctat
      181 tttcccacac gatacttgtt ttcagctctt ttttcaaaaa cacaaaactt agtattccac
      241 agtttccaca acacaatatg tagtgtttgt tgtttgactt ttttactaag agttttatac
      301 tatctattat agataataag aatgtgttag aggaggcact caccatgaac gtaaaaatct
      361 tttctaaaaa taattgtatg caatgtaaga tggtcaaacg gtttttaaca gaaaatcaaa
      421 ttgcttttga agaagtcaat atcgatgaac agcctgaagc agtagactgg ttgaaagcac
      481 aaggattcca aagtgttcct gtcattactt ctgatgcaga aacagttatc ggattccgtc
      541 ctgatcagtt gcgtaaactt gccagctaac atgactaaaa gaatcaaaac gtgatacatg
      601 gatttgaagg gcttgagaca gcaccagttt ttgtcgcaaa cttccaaatc cttttttaaa
      661 gcttaaaaac aatgcaatga tttgcaatca agcatcatca aatacgaaat gaaggttggg
      721 cgtacgtatg agtcttaaaa atttacaaga tgtcagttat ttcaagctga ataatgaaat
      781 caatcggcca gtcaatggtc aaatcccttt gaataaggat aaagaagctt tagttgcctt
      841 ttttgaggaa aatgtcaaac caaatacgat gacttttcta tctattatgg aaaaaatcaa
      901 ttacttgatc gatcaagaat atatagaagc agaattcatt caattgtatt caccatcatt
      961 tattgaaaag ctatatcaat ttttggacga gcaacatttt tctttcaaat cttttatggc
     1021 tgcttacaaa ttttattctc aatatgcgtt gaaaaccaac gacggttccg cttatctgga
     1081 aacttatgaa gatcgagtag ccttcaatgc gctttacttt gcgaacgggg atgaagaatt
     1141 agcacttgct cttgcagacg aaatgatcca tcaacgatat cagccagcta caccttcttt
     1201 cttgaatgct ggacgtaaac gtcggggcga attggtgtct tgtttcttga ctcaagtcac
     1261 tgacgatatg aactctatcg ggcgctcaat caactctgct ttgcaattgt cccgtattgg
     1321 tggcggcgta gggattactt tatcaaatct tcgtgaagcc ggcgctccta tcaaaggata
     1381 tgagggtgct gcaagtggtg tcgttccagt catgaaatta ttcgaagata gtttcagcta
     1441 ttctaatcaa ttaggtcagc gtcaaggagc tggcgtggtt tatttgaacg ttttccatcc
     1501 agacattatt gctttccttt cagcaaaaaa agaaaatgca gatgaaaaga ttcgcgtaaa
     1561 aactctttct cttggcgtga tcgtacctga taaattttat gaattgacac gtaaaaacga
     1621 agatatgtat ttatttagtc catatagtgt cgaaaaagaa tatggtgtcc cattctctta
     1681 tgtaaatatt acagctgagt atgatcatct agttgcaaat ccaaacattc gcaaacaaaa
     1741 aatcaaagcg cgtgatcttg aaaatgaaat ctcaaaatta caacaagaat ctggctatcc
     1801 ttacgtaatc aatattgata cagcgaaccg cgagaatcca atcgatggca agatcatcat
     1861 gagtaattta tgttcagaga ttttacaagt tcaaacgcct tctgtgatta acggcaaaca
     1921 ggaatatgaa gtattaggaa cggatatctc atgtaactta ggctcgacaa atatcgtgaa
     1981 tttgatgcaa agccctaact ttggtcaatc tgtgcgtacg atgactcgtg cattgacatt
     2041 tattacagat gcatctgata tcgatgtcgt accaccgatc caaaacggaa ataagctgaa
     2101 tcatacgatt ggattaggtg ccatgggctt acacacttat cttgcaaaag aacaaatgga
     2161 atatggctca gaggattcat tagactttac aaatatttac ttcatgctgt tgaattattg
     2221 gacacttgtt gaaagcaaca atatcgctcg tgaaagaaaa caagttttcc ataattttga
     2281 aaaatcagct tatgctgacg gcacttattt cgaaaaatac gtaactggag aattccagcc
     2341 aaaatcagaa aaagtcaaag cattgtttga aggcattttt atcccaactg ctgaagattg
     2401 gaatgctttg aaacaagcag taatgaaaga tggactgtat catcaaaatc gcttggcagt
     2461 agcaccaaat ggctcaattt catatatcaa cgatacgagt gcaagtattc atcctatcac
     2521 tcgcatgatc gaagaacgcc aagagaagaa aatcggtaaa atttattacc cagcgcctta
     2581 tctatccaat gagacgattc cgtattacac ttctgcttat gatatggaca tgcgtaaagt
     2641 aatcgatgtt tatgcaatgg cacaacaaca cgtcgatcaa ggcatgagtt tgactttatt
     2701 catgcgttct gaaatcccag aaggcattta tgaatggaag acaacaacga agcaaacgac
     2761 acgtgatttg aatattttaa gacattacgc tttccacaaa ggaatcaaat ccatttatta
     2821 cgtacgtacc tttactgatg atgcagaaga aatcggaagc aatcaatgtg aaagctgcgt
     2881 gatctaatat catagataga ttcgcttatt catattttaa ttaaaggagc attctcatgt
     2941 cagaaacata ttatgaagca ataaactgga atgaaattga agatatcatt gataaatcca
     3001 cttgggagaa gctgacagag caattttggc tagatacacg tatcccactt tcaaatgacc
     3061 tagatgattg gcgtacattg tctcaattgg aaaaagatac agtaggacat gtatttggcg
     3121 gtttgacact gctagatact gtccaatctg aaagtggcat ggatcagttg cgtaaagatg
     3181 tccgcactcc ccatgaagaa gccgttttga acaatattca attcatggaa tccgttcatg
     3241 ccaaaagtta ttcttctatt tttagtacac tgaatacgaa aaaagaaatc gaagaaattt
     3301 ttgactggac aaatacgaat ccttatctgc aaaaaaaagc tgagcgtatc aatgaaatct
     3361 acaaacacgg tacaccgcta gaaaagaaaa ttgccagcgt ctttttagaa acattcttgt
     3421 tctattcagg tttttatact cctctttatt atttaggaaa caacaaattg gctaacgtag
     3481 cagagatcat caaactaatc atccgagatg aatctgtaca tggaacctac atcggttaca
     3541 aattccaatt aggattcaac gagttacctg aagcagaaca agaaacgtta aaagactgga
     3601 tgtacaatct tctttatgag ctttacgaaa acgaagaacg ttatacagaa gaattatacg
     3661 atccaatcgg ctggacagaa gaagtcaaga cattcctacg ctataatgcc aacaaagcat
     3721 tgatgaacct agggatggat ccactctttc cagatactgc caatgatgtc aacccaatcg
     3781 taatgaacgg gatttcaaca ggcacaagca accatgactt cttctcacaa gtcggaaacg
     3841 gctatttgtt aggtcatgta gaagcgatga aagacgacga ttatttgatt ggattggaat
     3901 aaggatttat tttaattcaa aaatttttcg aaaaataaaa agagtttagc gtacagatat
     3961 tttaaatgta cactgaactc ttttttatgc ttattcactt gtttcttcca gcacttttgg
     4021 actgtctaac ttttccgaaa catgttccat tcttctcaat aaatagattt tccggatatt
     4081 taatattatc gtcttaatca ctgcatagac aggtatgcaa atcagcatgc ctaatattcc
     4141 tgcgacactt cctgctccaa tcaataccaa gatgatcgtc aaaggatgga tattcattga
     4201 tttcccaaat aacaacggct tgatcaaatt tccatctaat tgttggatca ctaaaatcga
     4261 caacgccata tacaaagctt gaaatggaga gataaacaaa ccgacaatga ttgctggtac
     4321 cgctccaata aaagggccga catacgggat aatattcgtg attccgcaaa tgaccgcaag
     4381 caacaagcta taaggctgat cgaatattct cattgccaaa aagctcatta caccaataat
     4441 caaagcatct aagatcgtac tgctgatata ggcagataat gtttcgttta attcttttac
     4501 cgtttggcgt aactctttac gaatcgcttt cggaaagaag tgagacaacg catcaataaa
     4561 tttgtgccca tccttgaaca taaatagcaa aataaatgga acggtaaata acaaaataaa
     4621 aaattgcatc ataaatgaaa tgatttttga gacacttgca gataaactga ctaatatgat
     4681 attgaacaaa ttactaagag ataaatttgc tgatgctaat tgttcttcca aattgaaatt
     4741 tttaaactct tcccgctgcg ccaattcatt taaccatttg cttgtttcat ctgcatacat
     4801 tggcaagctc tgggtcaatt gaattgtctg ttccattaac tgaggcacta cattcatgac
     4861 agttaaaaca actaaaatga ttacgaccaa aaacgtcaat aaaaaaccga acactctcgg
     4921 gatccgatgc ttttcgagaa aaacaacgat tggatcgaac atataataga taaaaccagc
     4981 aacaagcaat ggtaaaaaaa ctgccgaaag cattttcaat attggactaa aaatattcgg
     5041 catttgtaaa ataaagtaca cgccgatgat cgtgagtact agctccactg tccagaaaaa
     5101 taattttgat tgtttaaaac gtgattgcat acatctcctc caaggtcctt tgatacttct
     5161 attctatcat ttaacaaaga gttacgcgct gagattttca tttgcttaaa aaaaacaaaa
     5221 aaaataaaca tattttaatg agtagacgag tacttaaaca aaaacacggt ttggagtagt
     5281 gacaaaaatt atactgcagt ccctttttcc aaataaacgg tgttcaaaag cttgcatctt
     5341 cgtaataagc ccagccaaac gaaaaatata aggaactatt tttatttggc tgggcttatt
     5401 acttgatgta ggacatctat tttacaacct cagatcattt attgcgcaat actgactaaa
     5461 tgcaactttg tgatattttt gtgtacggac aactcctcga tcacatcggc ataagtgagc
     5521 ccttttggaa gatcaatcgt atagatattt ttgtacactc ggtagtcatc aacgaatttt
     5581 acgtcaaaat tcacatcttc gattacgata tttttctctt caaaataatc ggtaagaaaa
     5641 tctttggttt cttttctatg gataaattgg atttctaact ttttagtcgt aggtacatgg
     5701 atcacgcgtt tcactactgt gagtgcaata gcaatcccga taaaactgat gatcgcaatg
     5761 gaatagtaac ccatgccgat ggcgatccct aatccagcaa ttgcccataa agaagcggca
     5821 gtcgtaagtc ccgtcaccga ttgtttcgtg acgatgatcg ttcccgcgcc taagaaaccg
     5881 acaccactca ccacttgagc aatcagcctt gcttcatctg aacgaatcgt tccagcaagc
     5941 tgtggatact ctaaagcatc tcttaatgca ccactagcga tctcaacttg gatcaatgca
     6001 atgatacatg ctcccataca aactaaaata tgcgtacgca tacctgctgg acgattttta
     6061 tattgtcgtt caaaaccgat tgctccgcca aatagcatag cgatccctaa acgtaaaata
     6121 atctctggaa ttgtcaatac catatcattc atctaaacac cctccagtcc aatatatcat
     6181 gtctgaaagg agcagacaaa aaaagtgcaa tttttacctc ttacgaaaaa aggactgtga
     6241 caaaattttt gtcatagtcc tttttatgaa taaacggtgt tcaatcagct gaccctcggt
     6301 attactcaac aaacggcaaa atatggggag ctatttttcc tttgtcttct ctaatactca
     6361 ggtcaaaacg ctgatttcac aacctcttta gcctactatt gaaaattaca acttacttgc
     6421 tgcttcttca tcgatgatca caacaacatt gtcgtgattt tgcaatacac ttgctggcaa
     6481 atgattcgtt acaggtcctt caatcatccc tttgattgca tcagctttgc tttcgccaaa
     6541 tgccatcaag acgatttctt tacttttcat aattgaaccg attcccattg aaacagcttt
     6601 tgttggtaca tcttctgctt tatcgaagaa tcgtttgttt gcattgattg ttgattctgt
     6661 taagtcaact actgaagtcg ttccgtcaaa tgaagcacca ggttcattga aaccgatatg
     6721 tccattttgc ccgattccta agatttggat atcgattgga tgtgcatcga tgatttgatc
     6781 atactttttg catgctgctt ccagatcttc tgcttttcca tctggtacaa acgtttcttt
     6841 gaatggtttt ttatcaaata attggacatc catgaaatga cggtagcttt gttcatctga
     6901 accgcctagt ccaacgtact catctaagtt tacagaagtc atttcactga aatccaaatc
     6961 gcttgaagtc atttctttat ataaagtaac tggtgtgctt cctgttgcca atcctaatac
     7021 ttttgcacct ttgttcatac cttctttgat gatttcaaat gctttttttc cgccttcttc
     7081 agcgtttttt acacgaatga tttccatagt gattgctcct ttatattttg gtatagccca
     7141 attatagcat tctttttttc tttttacaat aattagtata gaccaaattg catttttttc
     7201 attttcttga caataaatag gaatccgtgt taggtttcat aagatggttc aatcaaacaa
     7261 tccatgttgc atgaaaacca agcaagtgat tcactgtgga cgctttatgt ctgcagtttt
     7321 tttatgaaaa aggagttatt atgagtatac tgacattaaa caacattacc cagcaatttg
     7381 gcgacaaaat cctctatgaa ggtgtcgatc tgcaattaaa tgctggcgaa catcttgggc
     7441 tgattggaca aaacggagcc ggtaaaagta cattgattaa aatcatcact ggtgagatct
     7501 taccagatga cggacagatc cattggcaaa aaaatattca caaaggttac ctcgatcaat
     7561 atgtggaagt cgatgaaaca ttaacaatcg aagaatttct aaaaactgcg tacgcaaaag
     7621 aatacgaaaa ggaagaaaaa attgcttttc tttaccaaac atatagcgag accttcgatg
     7681 attccttgct ggaaaaagct gggaaactcc aaacggaatt agaccaagga gtgttttatc
     7741 aaatcgacac cttagttgct gagatgagca gcgggttggg gatcgatgtc ttaggtttag
     7801 attcactgtt gaaaaatcta agtggtggac aacgttcaaa ggtgatttta gcaaaactac
     7861 ttttagaaaa cccagatgtt ctgatcttag atgaaccaac caatcactta gatgatcagc
     7921 atatccaatg gctgacgcag tttcttcagg attttgacgg tgcgtatatc gtgatttccc
     7981 atgaccaaga atttcttgat cagatcacca cccatatcgc agacatcgag tttggaaaga
     8041 ttactaagta tactggccat ctaaaacaag cattgaaaca aaaggaacaa aatcgcgaaa
     8101 gctatttgcg ccaatatcat gctcaacaaa aacaaatcga aaagaccgaa gcttatattc
     8161 gaaaatacaa agccggttcc cgatcaacga tggcaaaaag ccgacaaaaa caattagata
     8221 aaatcgaacg gctgacgcct ccagcttcag caagtaaagc tgtctttgat tttccattct
     8281 cgcctattgt cacgacatta gcagtggaaa caactgattt agtcatcgga tatgatcgac
     8341 cgctacttgc acccatcaat ttgaccatcc gctttggtga aaatattgcc attcgaggct
     8401 tcaacgggat tggaaaatct actttattaa aaacactgat cggtgagatt gaaaaaataa
     8461 gtggcgattt ccacttccca gataacacaa aaatcaatta cttttcacaa gatctgtact
     8521 gggaaaatcc attagaaaca ccactacaat atttaagcag tcaatttcca aaagcgacta
     8581 tcaaagaatt acgtcgccaa ctagctaaag cagggttagt taatcaatta gcaagtgaac
     8641 ctctcactac attaagtggt ggggaacaaa ctaaggtgaa acttgcacag ttgacaatga
     8701 accaaggtaa tttactgatt ctcgatgaac cgacgaacca tatcgatcaa gaaacgaaag
     8761 aaagcttaca agacgggata cagaaatttc caggaacagt aatcatcgtc tctcatgaac
     8821 aagaatttta tcaagatctt gtgcaacgag tcattgagat tgaaccaaaa taaacgtttg
     8881 attgaatagt tgaaaaaaag gagctgtgac aaaagttttg tcacaactcc ttcttctgaa
     8941 taaatggtgg ccaattagct gaccttcggt attagctcaa aaaacggcaa aatatgagga
     9001 gctattttta tttggctatt cttattactt gatgcagaac agcttttcac aacctctttt
     9061 cgtctatttt ttattttcga gatttctctt tcctttaaaa acaactgttt gaaagaagaa
     9121 cgcacatcct tgaaaaacaa gcccgctaac cgttacacct aaccagccat actgctgcca
     9181 cattgtcgtc cctagcaatg aaccagccgc acctccaata aagtacataa acataaatac
     9241 agtattattt cggttgcttg ctgcttcacc tagcccttgt actcgtgttt gattggcgac
     9301 ttgaccaaat tgattcccga cgtctaataa aataatagct aaaagcaaaa tggccaaatg
     9361 gcttccgcca accaataaag caataaagct tattgtttgc ataacaagtc ccatatacac
     9421 gatttttctt tcagaataag aatctgccag tcgtcccacg ataggtgcag caactgctcc
     9481 agacaaacca aaaatcgcta atatccctgc ttcgaatgtt ccccaattgt aggcgggact
     9541 gctgatataa aaaatcaacg tagaccaaaa gatcgaaaat gttccaaaca tgaaaaatcc
     9601 actgactgcc gcttctctta atagacgttg agaagcaact aaatgcggca aacttttaag
     9661 tgaatcaagg tatgaaacag ctgtactact ttgctggaca gaacgcggca ttttccagta
     9721 aagcagaagt gtaagaattc cgactgcaca taaagcaaat aaatagatca tccgccaaga
     9781 aaaaagactg gcaacaatac cagataccgt ccgagaaagc aaaatcccca cgagtaaacc
     9841 gctaagcaat gtacccatga ccttccctct tgcttttggt cccgctagta ccgcaccgta
     9901 aggaatgatg atttgcggca cgatcgaaag taatcctatt aaaaaagaag cgcatgcaaa
     9961 taacgtaaac gaaggcgcaa aaaaagcggc caacagagag acggcagata acgctgccat
    10021 tcgaatgatc aattttggac gatccaccac gtcccctaaa ggaactaaaa acagtaaccc
    10081 taacgcatag cctaattggg tcaacatcgt caatatcccg actgcacttg tgctaacaga
    10141 taaactagct gctacttttg ttccaatcgg ctggatgtaa tacatattgg caacaacaac
    10201 gccacaagtg attgataata gtaaaataag cgaagttgtt aaaggttttt tttcattact
    10261 cttcattgat ttcccccatt tcctatctta gtttacaact tatggaaaag attggaaata
    10321 gaaaaataca actattcgcc acacttatta tttgtgcttt tgttttcctt gttttttctt
    10381 gcgatcttta taaatgaaga caactaaccc tactagcaca gtaacacttg caaccaacgt
    10441 gactgcagtt ttgttatatc cgtgaaacag agcttctcca ccacacgcat acaacacaga
    10501 cgaagggatg actccaatca cgatcgacaa tatgatttgc tgtagaggca atttcatcgc
    10561 atctgcagca aaattgacca tagaagatgg aataaccggt atcatatagc ctaacattac
    10621 acctattttt ggatgtttca tttgtcggat tgcttgcacc caatgattcg tttcggtttt
    10681 cttatctaaa aattttaagc gatgcataag gaaaattgca gtgatgttac caagagcatt
    10741 acccaaaatg ttgatgatcg cacctgtcag tggtccaaag ctcaaaccag caatgactcc
    10801 aactactgaa gtaggaatac ctggaacagc acacatcacc gctgtcaata cgatcaagag
    10861 tatcgatgca aaagtcccat gcgaacgaat atgtgcgagt agctctgcct tactcgctct
    10921 cggatttaaa aataattgga tgtctgtctg atattctaaa tacaaccgat agcccactac
    10981 cagtatcaga atgattccca acgagatcaa tatcaatcgg attattttct ttttcatatt
    11041 tcctcctgaa aaaaatctat attcattaaa taatagttta acataaaaac aaaaaagcac
    11101 cgaaatccaa cataggatct cagtgctttt aaatagtaaa aattatttaa cagttacaga
    11161 tgcgccaact tcttcaagtt tagctttcaa ttcttcagct tcttctttag atacgccttc
    11221 tttaactggt gcaggagcgc catcaactac agctttagct tctttcaagc ctaaaccagt
    11281 tgcttcacga actgctttga taactttaac tttttggtct ccagctgcag ttaattctac
    11341 agtgaattct gtttgttctt cagcagcagc agcgccacca gcagcagcta cagctacagg
    11401 agcagcagca gatacgccaa attcttcttc aatagcttta actaggtcgt taagttctag
    11461 gatagttgct tcttttaact cagcaacgat gttttcaatg ttcaatgcca ttataatttc
    11521 ctccgtttaa ttgttctttt ttatttaatg ctttatgcgg caattaagct gcatctccat
    11581 cttctttgtc tgccactgct ttgacagcgt aagccacgtt gcggactggc gcttgtagta
    11641 cagatagcaa catagataga agtccttcgc gatttggcaa ttttgccaat gctgtgattt
    11701 cttccaatga agagacttta ccttcgatga taccgccttt gatttctaat gcttttgctt
    11761 ctttagcaaa ttcgtccatg attttcgctg gtgcgactac atcttcgtta ctgaatgcaa
    11821 cagctgtagg tccagtgaat acttcgtcca tgccttcaag gccagctttt ttagctgcac
    11881 gtgaaagaat agaatttttg ataactttca tttctacgtt agcatcacga agttgtttac
    11941 gtaaacgtgt tacttcatca actgttaatc cgcggtagtc aacgactact actgatgcag
    12001 ctgctgtaaa tttttcgctt acttcatcaa cgattgctgc tttttttgcg ataattgctt
    12061 cactcatttt tgagtttcac ctcctgaatt ttgatggaag agttttttgc actgaaaagg
    12121 caagtcactt ttatagttac tttgctcaac cagtattatt catcacctat tcacttcgtt
    12181 catagtgatt ctactcgctt tgctcgaaag gaaactcctt tcgctttgct caaccagtat
    12241 tgttcatcat ctcttcactt cgttcatagt gattcacaat aaaaaactct atgccaccgc
    12301 agacatagag ggactattga atatattcaa taaacgtcct cggtaggaaa ttaaggcttg
    12361 atgccaccta ctgtcttcgg tacaagttaa ttattaacct caactagctt agcatgctaa
    12421 actagttgag tcaagaatta tttttcagtt ggatcaatca acattctaat atttaattag
    12481 aaagaagctt ggtcaacatg gataccaggt ccaaatgttg ttgtgatcgt tacatttttc
    12541 atgtattgac ctttagctgc tgatggttta gcttttagca atacatcatg gattgctgca
    12601 aagttttcaa ctaatttttc atcgtcaaat gaaacttttc cgattggtac atggatattt
    12661 ccagctttgt ccacacggta agttacttta ccagctttca cttcgtttac tgcttttgtt
    12721 acgtccattg taactgtacc agttttaggg tttggcatta agccttttgg tcctaatact
    12781 cgtcctagac gacctacagt agccatcata tcaggtgttg caacaactac atcaaagtca
    12841 aaccatccgt tttggatttt ttgaaccatg tcgtcgtcac ctacgaaatc tgcaccagct
    12901 gcttcagctt cttttgcttt gtctccttta gcaaaaacta aaacagtttg tgttttacca
    12961 gttccgtttg gtaatacaac agcaccacgg atttgttggt ctgctttttt agggtctacg
    13021 ttcaaacggt atgcaacttc aactgttgca tcaaattttg caatgtttgt ttcttttgct
    13081 aaagctactg cttcggcaac aggataaact ttacttgaat caactttttt taatgcatct
    13141 tgcatttttt tgctcttttt agccattttg gttcctcctt gattgtggtt ataacggttg
    13201 tacctcccac gtgttctata tctttcaata tagagccaac tgagaagctg ctgtcttagg
    13261 gtgtatagat tattctacag tgatccccat gcttcgtgca gttccttcaa ccatgcgcat
    13321 tgctgcttca acgttagctg cgtttaggtc ttccattttt aattcagcga tttctcttac
    13381 ttgatcgcta gaaactttcg caactttatt tttgtttggt tcgcctgaac ctttttcgat
    13441 tttagctgct tttttcaata gaacagcagc tggcggtgtt tttgtaacga atgtgaatga
    13501 acggtcttcg tatacagaaa taacaaccgg aatgatcaaa cctgcttgat cagctgtacg
    13561 agcgttgaat tcttttgtga atcccatgat attgataccc gcttgaccta gtgcaggacc
    13621 tactggtgga gctggagttg ctttacctgc aggaatttgc aatttaacga ttttttctac
    13681 tttctttgcc acgagacata cctccttaag tccgtgatgt ggttaattgg agatgcatat
    13741 ttctcctccc acgttttatc tgatctgaaa aaatccagac aaataatgtg caaaacaatg
    13801 cacactggta cagtatagca aatttatttg ttttttcaag atttttttct gctattttta
    13861 cattttgctt gaataaacgc taaatttatt ctaaaaaaac aacaattttt cgttctcatt
    13921 tcttctttta tgttacactt gcgctagcat ggttcaacag atgaaagcaa acaataacac
    13981 ggttttttag acccttgtac aaagattcct acataacaaa atgaggtgga gaaaatgaaa
    14041 gatttaaggt atagaagtgt ctttgatatt attggacctg tgatgattgg tccaagtagc
    14101 tctcacactg ccggagctgc tagaatcggg aaaatcatac gtagtatatt tggcgaacag
    14161 cctgattctg tagatattta tctatatgaa tcatttgcaa aaacctatcg ggggcacgga
    14221 acagatattg cattagttgg cggcctgtta gggatggaac ccgatgatga acgtttagct
    14281 gattctttga aattggcata tgaagccggt atggaagtct tgtttgttcc taagagtgaa
    14341 aaagccgaac atccaaactc tgtaaagatg cttctaaaaa aaggaacacg aaagctttca
    14401 gtcactggca tttcgatcgg cggtggcaat atccagattt ctgaattgaa tggattcaaa
    14461 atttctttaa gcatgggtac tcctaccttg atcgttgtcc accaagatgt tccaggtatg
    14521 attgctaaag tgactagtat actttctgag accaatacga atatcggcac tatgacagtg
    14581 acacgggaat caaaaggtga aaatgccatt atgataattg aagtagatga tcctcaagta
    14641 gaagaaacgg taaaaagact caaacagctg ccaaatatcg acagcgtaaa ttatttcgat
    14701 taaggagcta aaaaaatgtt tttatcgatt gaagaattag tagatcaagc aaaagcttat
    14761 ccaagtgtag cagaattgat gattgcagtt gaaatggaaa tgagtggccg ttcccgtagt
    14821 cacatcatcg agatcatgga aaaaaacctc tcagtaatgg aacaatcaat caacgaggga
    14881 aaagaaggtg ttacctccgt cacaggaatt accggtggtg atgcaaaaaa attagatgat
    14941 tatttaaatg aggcagactt tttaagtgga gaaacaatct taaaagctgt acgcaatgct
    15001 gtagctgtta atgaagtcaa tgcaaaaatg gggttgattt gtgctacacc gactgccgga
    15061 agcgcgggtg ttgttcctgg tgtgctgatg gctgtccgag atcggcttga tttgacacat
    15121 gaacagcaat tggaattctt atttaccgct ggcgctttcg ggttagtcat cgcaaacaac
    15181 gcttctatca gtggtgcaga agggggatgt caagcagaag ttggttctgc aagtgcaatg
    15241 gcagcagcag ctttggtttg tgcaaaaaat gggactcctc atcaagcagc acaagcagta
    15301 gccattacat tgaaaaatat gatgggactg atttgcgatc ccgttgcagg cttagttgaa
    15361 gtcccgtgtg tcaaacgtaa tgctttaggc tcttctcaag cttttatctc tgctgatatg
    15421 gcattagcgg gtatcgaaag tgtcattccg ccggatgaag tcgttgctgc aatgtaccaa
    15481 gtcggacgtc aaatgccgtc gattttcaaa gaaacagcag aaggtggctt agctgttacg
    15541 ccaactgcta aacgacttgc ggaagagatt ttaaataaga gggcgtgata aagctcctta
    15601 atttcgagta ttaagagatt agttatcttc ataacaaaaa acgaagagag gactaattgc
    15661 ccttctcttc gttttttatt aatccatcaa tttcgtattt tttacttcat cgatcgtttt
    15721 tgttcctgca agttgcatcg tgatcgacaa ttctttgttc aggtgttcaa aaacagatgt
    15781 tactccttct gccccaccta gattcaaccc ataaatcact ggtcggccaa tcgcaactaa
    15841 gtcagcacca cttgccagag ctttgaagac atgctctcct cgacggacac cagagtcgaa
    15901 aatcactggt acacgtttgt tgactacttc ggcaattttt ggcaatactt caaaggacgc
    15961 aggacctcca tctagttgac gtccaccatg attggatacc cagataccat ctgcccctgc
    16021 agagatagcg attactgcat cttctggcga ttgtattcct tttacgataa ctggaagatg
    16081 ggtgatttct ttgattgttt tgatgtcatc tggtgtcaag ccttgttttg cagctgcata
    16141 aatttctgcg attccttttc cttcgccatt accacttgca ctttgttcgc tatacgcagc
    16201 taagttaggc attggtaatg gaaattggaa ttgattgatg acgtcttctt cacggtaacc
    16261 accgagcgtt gaatctgccg ttaaaatgat agctttagcc cctgcttcta ctgctttgtt
    16321 caaaatgaac tcattgaatc tgtcgtcttt actcatatat aattggaaaa actgcggtgc
    16381 gcctggcgct gcatcggctg cgtctttgat tgtcgtattt gcataagtgc tgatggaaaa
    16441 gattgatcca gcagcagcta ctcctttagc tgtatcagct tccccttttt catgtgccaa
    16501 tccttgggcc gctgatggcg cttggataat cggtgtatct aattcaattc cgaaaacaga
    16561 tgtatgcaaa tcggctgagt cgattccgcg taatatccgt ggcatgattt tctttgtatt
    16621 aaaagaagtc gtattttctt tcatcgtcca ctcatcttca gaaccaccgc gaatatagcc
    16681 aaaagctcct gcttccatgc gctcttttac acgaccttct agttaggcaa tgtttacaat
    16741 atcgattgga tgttcttcat tacttgtatg ataagtcata gtagatcctt ctttcatctt
    16801 cttgattaca tataaatcat actaaactta caaaaagtaa gcaaacattt actctcagat
    16861 acttttatcg ctattctttc caaataaatc gacttacaag ctgatctact tcttggtttt
    16921 catgaagcat actatgagag gcattttcac ctgtcactac tttttcctct atttcatttc
    16981 cgcgttgctt caacaacgca tagaccgcta atgcactgtt taacggtact gttccatcac
    17041 tcaagtctgt ttcatctagt tgaccagcaa tcaagaaaaa tcgagtggtt ataggaatat
    17101 taccgatcat ttgctggtaa tcttgatagc ggttagaaac gacagcaggc ccctttgcta
    17161 actcatctgt cagagactgc gcactatcat cgacaaaatc attaaaaggc gcgccaattg
    17221 ctgttaatgt ctgaacttgt ggcaatgacg tatcctgtcc atacgttcct aaatagcgta
    17281 gtgcactaac tcctcccatg gaatgcccca ctatgttgac tttatctatg ccgtagtttt
    17341 ctttcagata caaaagacaa gttttgatcc attctgcttg attccactcg ttatttttat
    17401 tatcttcaaa caatacttgg accatcggat tgtctgcttt tccagacaaa tctcctgttg
    17461 cttgtatttg tccatctgtt cctactgtta aaaccaattc ttttactgct tgctgatttt
    17521 tttccaaacg ccgcaacatc cctttaaagg aattttctgt cccgctgtag ccgtggaaaa
    17581 aaagtgttgg aatagtggtt ttttctatca ctttactgga ttctgccgtt ttttctttat
    17641 cagaaacttc agtagtattt atcttcgtcg tctggcaacc acttaacgca aacaaactga
    17701 taaggactaa agctgctgca gttgcacgtt tgcataaatt tttgattggt tctgtcatac
    17761 acctcttcct ttccatatag aaaaaagaaa gacttgaaag gccaatcata tgatcgacat
    17821 ttcaagtctc tttattaatc gattttatct acttgatcga aatcaagttc tgtactggtt
    17881 tcacgtccaa acatatcgat gttgactttc aatttttctt tttctccgtc aacttctgtt
    17941 acttgtcctt ctaatccagc aaatgcgcct tcgatgattt tcacgacttc gccaacttct
    18001 acttctagat cattttgtcg caggctcata ccgatagaac gcaagatatg attgacttct
    18061 tctggaagta atggtgctgg tttgcttccg gctccgtgtg aaccaacgaa tcctgtcaca
    18121 ccaggtgtgt tgcggacaac ataccatgaa gcgtctgtca tgatcatttc aacaagtacg
    18181 tatcctggga atgttttatg aacgatttct ttttctttcc catttttgac ttcagtttct
    18241 ttttcttcag gaacaacgac acggaaaata aagtcggcca ttttcatact ttgcgcacga
    18301 gattcgatat ttgcttttac tttgttttca tatcctgaat atgtatgcaa gacataccag
    18361 tttttttcaa aagattccat tgaaggctcc tcatttcatt gtaaaataaa aaaacctcag
    18421 tacacccgaa gtttttcttt cgcttcatta tagcactaaa agtacgttgt ggctagcgag
    18481 ttattttaaa atccagccaa ataagctttg gatggctgta tccattacgt aaaacatcaa
    18541 tccgaaaata atagaagttt cgatgactac taacgtatct ttacggagtt gttttctgct
    18601 tggccaagtt acttgtttga tttcatcttt tacgcttttt agaaatttca tgagacaacc
    18661 tcccttgcta ctgtttattt cgtttcttta tgtaatgtat attgattaca atatttacaa
    18721 aacttattga tttccaaacg ttcgccgctt tttccttcac tgactgattt tgtgtagttg
    18781 cgagacccac aaacggaaca agcgagtgct gcttttttct ttgccattta cttctgcccc
    18841 cttgctgaga atcagactta tacttcataa cagtatcacg taacttttta cttgtcaatt
    18901 tgtgaaataa actatctatt gttttggata aaattatgat atgattaatt tgaaattaag
    18961 ctaaaatgag acaattggag gtatagcgat gctattaaaa acacttgtat ataagaaaaa
    19021 agatctgaca accgttacag agactagtac attagaagaa gcattaaaaa ttttagaaac
    19081 ttccggatac cgttgtgtgc cgatcttaga ttcttcaggt aatattttcc gtggaaacat
    19141 ttacaaaatg catatctatc gtcataaagc aaatggcggt gatatgacgc ttcctgtcac
    19201 tcatcttttg aaaaacgcta caaagttcat ttacctggat acttctttct tcaaagtatt
    19261 ctttacgatc aaagagcttc cttatattgc tgtccttgac agtgaaaatc atttttatgg
    19321 tattttgaca catagtacgt tgcttaatat gctctctcaa tcttggagcg tagaacaagg
    19381 aagctatgta ctgacaattg cttccacagg aaagcaagga gatttggctg ctattgcaaa
    19441 aatcatttca aaacacagca gcattgccag ctgtattact cttgatatcg aaaaagacga
    19501 atacattcga cgtacattga tcacgctacc ggctaacact gaagcgaaag tatgtgaaac
    19561 gatcgtcaac cacttagaga aaaagaatta taaagtggta gaaattgaaa atttgcaaaa
    19621 ccaagaagca taatcaaaaa gcgaaaaaag aaaacagcta atctgctttc ttttttcgct
    19681 ttttataatt agacacgctt ttcaaaatca agcgcgcgat ctaaataata catcaatggt
    19741 gctgaaacag ccatgacgat aaactcactt aatgcaagcg tgccataggt tgtccaaaat
    19801 gcttggccac ctgttggaac taacatataa gcaatcagtc ccatgctgat ggtaaagaag
    19861 atcgtgttca atactaaacg catctttaca tttttcactt tgttttgcaa taaagctgtc
    19921 aggcctaaag aaagcaatgt ttggccgcca ccaaaaacca catccatgat accaaaacca
    19981 aagaaggcat tatagacgat gaccccgcct aaaactcccc aaagcaattt acggttaaag
    20041 acaactaaat gattcaaact ttctgaaata cgaaactgga tcggccctga ggatacaggc
    20101 gcaactaaaa tagttaacgc tagataaaga gccatgatga tgccatttac aacgattacg
    20161 gataaccgtt gtctgctttt cgttttttgc attttctctc ctcttttctc ctagtttttt
    20221 taacgcggga tggttgatga accgcggtta tgtgaaacaa acaataatta taacagactt
    20281 ttcatttatt tcaatccgtc ttatctgctt ttaaaaaagc aaaagcttaa acaatgtaaa
    20341 cgtttgctaa aaatcgtggg atatggtata aagtaagtgt aaggggtcat aagaattccc
    20401 agtaaagcag acgaagtagg atctgcaaga acagaattgt tgtgaaactt aaataaataa
    20461 aaaaggatgt gggaatttga gcaacctctt actagagaac ggtctagcaa gcagctgaag
    20521 atctaagtgg acatgccgaa ccttggaact tctccttacg taagtaatgg atcatgcgct
    20581 tgctaggctt ttttgtgttt ttcggtcttc ggaccttttt cattttccta gacttctgct
    20641 ataataacca agaattaatt ttgtaacaga tttttttgaa aaaacaagtt tctttcattc
    20701 aaaaaataga aaaagcttgc taattattgg ttaaccagca aacaggagta ttaaaatatc
    20761 ttgttttatt gaatagcagg aggaaatcat gaaattccat tatcatatat tgatcaatcc
    20821 atcggcaggc agcggaaatg gctataaagt agcagaaaga attttaccgg tattaaaaaa
    20881 taagcatatt gactatacac ttcattatag tgaatacaaa ggacacgatg ctgagattgc
    20941 tgaaactttg gcaaaagaaa cattgattcc ttggatagaa gaagaacaga aaacaatcga
    21001 tactttcccc cttttgatca tcgtaggagg tgacggcacc ttacatcagg tacttgatac
    21061 tttctatcag atggaagtag aattcccagt tgcttatatt ccagcaggtt ctggagatga
    21121 ttttgctcga ggggcaggcc ttccaaaaaa tcccaaaaaa ggacttcagc tgattttagc
    21181 agctcaatca ccagaaaaag tccacataat ggcttatgaa gaaaaaataa gtgaaaaaaa
    21241 aggaatagct gttaataatt tcggtatagg attagatgca gctatcgtcc atgcgacaaa
    21301 tcattcttcc acaaaaaagc gattgaacaa atacaactta ggatcatttt cgtatttatt
    21361 ctctattctc cgtgctttat tcactcaaaa aggatttcca attctggtag atgcaggagg
    21421 aaaacaatta agtttttcaa acgcattttt atgtacagca acgaagcatc ctttttttgg
    21481 cggaggaatc gcgattgttc caactgctga tgcgtctaaa ccagtcattg attttgtcgt
    21541 ggtcgaacga atcaatttct ttaaaatctt gtggctcatt cttttactta tacagaaaaa
    21601 acaattgaaa tcgaaatatt tccatcacta cacaacaaac agattacgta tcgtttcaac
    21661 aacaccgcaa catggtcaag aagatggaga agaaatggaa aaacgacctt ttgatatcac
    21721 aataaccaca actaaacagt tgttttggtg ttaataaaag aggttttgtc agaagctaca
    21781 tctggaacac cgtttattcg gaaaaaagga ctgtgacacg acttttgtca cagtcctttt
    21841 tttactatgc agttaattct ttgcccaatt gggttgccat cgatagtttc tttctttacc
    21901 caaaataaat tggatcattg aaaacaccgc agcaaaagca actaagacac aaccattgaa
    21961 ccaaaaagat tctgcaaatg tttgattgta actgagtgtt tcactgatca agttattatt
    22021 ttgaacaaac caatatgttg gtgtgaatgc cgctattttg ctaaccattt ctggcaagta
    22081 ttctgctggt acaaacaccc caccgataaa gcaactgcct gtgataaaaa tatctttgat
    22141 cccaccgctg gtttctgagt tttttaccaa tgaagtgata caagccgaaa agctaatgat
    22201 cggcaataaa aacaagaaag tatttaaaat atgataccat accgttatat cccacgaggt
    22261 gtggatcact aacagaacca ttagaagtaa gaagccactg aacaacagca gcgagtaact
    22321 aatcaaacta aaggttactt tgcgattcaa gtttcgtttg gtaatgggcg cactctggtt
    22381 tcgtttactg atctctttgc gattaaatgc tagattgatg acgctgaaac cgctaaaaat
    22441 ggatagaaac aacccataag acaagaaatt aaaggttgtg cccgtcaaca gctttttcat
    22501 ccgctgacta tatcccgcac ttagctcaac ttctccagta accgagagtg ccatttgtgt
    22561 ctgttccata gctgcatcga ttgttttatc ttcttcttga aacagaatca gtgtttgcaa
    22621 aaaggtgttc acttgctgtg taacaactgt tttagaaaag gcatctgggc gactctgtat
    22681 cttgatcgtc ggcatctttc ctacttcgac ttgttcggta aagtctgcag gcagatgtaa
    22741 gataatgttt acacgattaa aataaagagc atcatcgatt tctttttgtg aggttccatc
    22801 caatgtaacg actgtttcct tgtcattcaa atatttacga aggccatcga ctacaggcga
    22861 ctcttcttca ctaatcaagg cgattttcgc atcttgcaga gaggtatttt caacatcaat
    22921 attttgcgca aaaaagaatg tggcaacaac agtgataatt acacctatca gtaaattcga
    22981 tttatttgct ttaagcaatg tccaaatcgt tttataaagc gtcataacgc acccctcttt
    23041 ccatccaagc atttcctaac gcaaaaagga aaataaatcc gatcaaccat agaagattgg
    23101 tataaaaact agccaaatca gtgtagaaat aaagttt
//
LOCUS       DAYYCY010000024        22940 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000024 DAYYCY010000000
VERSION     DAYYCY010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22940
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.024"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(248..2629)
                     /locus_tag="ACHAZB_000802"
     CDS             complement(248..2629)
                     /locus_tag="ACHAZB_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFM8613494.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2619..3266)
                     /gene="recU"
                     /locus_tag="ACHAZB_000803"
     CDS             complement(2619..3266)
                     /gene="recU"
                     /locus_tag="ACHAZB_000803"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HFM8613495.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3330..3869
                     /locus_tag="ACHAZB_000804"
     CDS             3330..3869
                     /locus_tag="ACHAZB_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HFM8613496.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            3959..4378
                     /gene="gpsB"
                     /locus_tag="ACHAZB_000805"
     CDS             3959..4378
                     /gene="gpsB"
                     /locus_tag="ACHAZB_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HFM8613497.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYEAYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4400..4767
                     /gene="rnpB"
                     /locus_tag="ACHAZB_000806"
     ncRNA           4400..4767
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHAZB_000806"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            4976..6142
                     /locus_tag="ACHAZB_000807"
     CDS             4976..6142
                     /locus_tag="ACHAZB_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HFM8613498.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6188..7684
                     /locus_tag="ACHAZB_000808"
     CDS             6188..7684
                     /locus_tag="ACHAZB_000808"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HFM8613499.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7765..8448
                     /locus_tag="ACHAZB_000809"
     CDS             7765..8448
                     /locus_tag="ACHAZB_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFM8613500.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            9093..10283
                     /locus_tag="ACHAZB_000810"
     CDS             9093..10283
                     /locus_tag="ACHAZB_000810"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFM8613501.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            10385..11338
                     /locus_tag="ACHAZB_000811"
     CDS             10385..11338
                     /locus_tag="ACHAZB_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFM8613502.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            11407..12756
                     /locus_tag="ACHAZB_000812"
     CDS             11407..12756
                     /locus_tag="ACHAZB_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFM8613503.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(12939..13262)
                     /locus_tag="ACHAZB_000813"
     CDS             complement(12939..13262)
                     /locus_tag="ACHAZB_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8613504.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            13391..14554
                     /locus_tag="ACHAZB_000814"
     CDS             13391..14554
                     /locus_tag="ACHAZB_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFM8613505.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            14624..15973
                     /locus_tag="ACHAZB_000815"
     CDS             14624..15973
                     /locus_tag="ACHAZB_000815"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFM8613506.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(16018..16449)
                     /locus_tag="ACHAZB_000816"
     CDS             complement(16018..16449)
                     /locus_tag="ACHAZB_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8613507.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            16837..19479
                     /gene="alaS"
                     /locus_tag="ACHAZB_000817"
     CDS             16837..19479
                     /gene="alaS"
                     /locus_tag="ACHAZB_000817"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFM8613508.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            19679..20275
                     /locus_tag="ACHAZB_000818"
     CDS             19679..20275
                     /locus_tag="ACHAZB_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFM8613509.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            20397..21545
                     /locus_tag="ACHAZB_000819"
     CDS             20397..21545
                     /locus_tag="ACHAZB_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFM8613510.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(21583..21996)
                     /locus_tag="ACHAZB_000820"
     CDS             complement(21583..21996)
                     /locus_tag="ACHAZB_000820"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HFM8613511.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            22075..22506
                     /locus_tag="ACHAZB_000821"
     CDS             22075..22506
                     /locus_tag="ACHAZB_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HFM8613512.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(22605..22838)
                     /locus_tag="ACHAZB_000822"
     CDS             complement(22605..22838)
                     /locus_tag="ACHAZB_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8613513.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
BASE COUNT         7384 a         4034 c         4794 g         6728 t
ORIGIN      
        1 actaaataaa agactagtgt gtatttagcc gatttaatta taaacaagta aatggataaa
       61 gtatatgtaa tgataaaaga aaaatggatt ttctaatttt taagctaaat gttagatcac
      121 acatgtatat gctggatagt cttataaaaa atcatgtaat acagcttggt acatgatttt
      181 tatttgtagc tgttttttac atagacacaa aaaagaaacc acgcttgtag acgtggtttc
      241 ttttttacta tctgtttcca gaagctgagg tgctgctgga tggtgtatgg tcatttgcag
      301 catccgctcc gctagttgct ggctcactgg aagaagcagg aggagcagat gaagacggtg
      361 tcgagctttc ttcggatgaa gaagaaggaa ccgttgttga agacgctggc tcgcttgttt
      421 ctgctgtcgt gctttctttt gaggactcag cagttgattc gctttgacta gtagagctag
      481 agctttgagt cgttgtctct gttgttgagc ttccaggagt ctggacatag ctggaagatg
      541 gaatagttgt tgatggtgta attgcattcg atctagctgt atattgatct ttgtagtaca
      601 gttcaccgcc cacacgaatt aatccgcttg gcatctccca gtctgtattt gtcacgttag
      661 ctgaaacata ctgcataagc tcgcgataaa catcagaggc tacgtgtgac gattctgaag
      721 tgacaggtgt cattttttta ttgtaacctg tccacacgga aatcgaataa tttggcgtat
      781 agcccgcaaa taggatatct ggatacaccc cagaagaaat accaagtttt gcatactcgt
      841 catctgtgta attggatgtt ccggtcttac cggcttgata taacccagcg atttgcgcat
      901 tcgtaccagt tccttcagtg atcgtatctt ttaaaatgtc tgtgatcata taagctgttt
      961 ctggagacat ggctgttttt ccatctggct cataagtttc ttctgttccg tcttggaaga
     1021 cgattttatt aacatattga ggtttgtaat aagtccctcc attggcgaaa gccgcatagg
     1081 ctgctgccat ttttaaggaa gaagctccgt attttgtccc atcttgttct tccgtattac
     1141 tagagatcgc attcgattga tggatcgtac tgtattcgat cccaagattt tttaagaaag
     1201 aagccacttt atctgaaccg acttcattga ataatttgac tgctggaaca ttacgggata
     1261 agtaaagtgc ttggcgtaaa gtaatggtgc ccatatattg attatcccag tttcctacag
     1321 gagtcgaagt cccttcatag ttatacggtg cgtcagtaat agtttttcca gtagaatatt
     1381 tcaaatattc aaaagctggt ccgtaatctg tgacaggttt catcgtagaa ccaaaatcac
     1441 gggaagtatt cactgctaag ttgttgccta aagttacgtc ttctgcgatg tgacgtccgc
     1501 caatctgggc ttttactttc ccggtattgg tgtcgataag ggtggaggca acttgcatct
     1561 cgtcgtctgg gtaagagaca tactgatccg tgttgacgat atcatatagt ttcttttggg
     1621 cgtccagatc caaattcgta taaatctcta aaccgtctgt gtagacattc ttatctgttt
     1681 tttcttgtac ttcattgatg acttctttga cataattatc gacgatctta gtattgtcat
     1741 cggattgagt tagttcttgc agaccatccg ttaccggtac attaacagct tgatcatatt
     1801 ctgtttgaga gattttttcg ttttgaagca tcgtatacaa aacagtatct cgtcttttct
     1861 tcgcctgatc tggataaaca taaggatcat aggcggaagg ggcttgaggc atcccagcaa
     1921 gcaatgctgt ttgcggtaaa gaaagttctg ataatttctt accaaagtac atctcagaag
     1981 ctgtctccat cccgtacaag ccgtttgaca tatacacttt atttacgtag taggtcaaga
     2041 tctcttgttt cgattttttt tgttccaatc ttacagccat ccacgcttct tgggccttgc
     2101 gtttcagagt ctgatcctcg gcactggtgg agaagaagga aagtttgatc aattgctgtg
     2161 tcaaagtact tccgccttgc agtccgccag aggtaaagtt agagagggca gaaccgatga
     2221 tccgaatagg gtcaaccccg atgtgtttat aaaaacggcg atcttctacc gacacgatcg
     2281 catcttctaa tgttttcggt aattcattcg cagaaatttt ttcacgcttt tctgcgccta
     2341 aatcttcgaa tagttcgcca tcttgtgtat atagtttgga agatacggta gcgtctaatt
     2401 ttttatccgt caattcgggc gcatcttttg cgtaatacca gaaaagtcct acacctgcta
     2461 aaaagaggat acagaaaaat gagagtattc ctaaaaggat cttgataaat aatccttttt
     2521 tatgtgttcc agaggacttt ccagaacctt tgccagaatt cttcttcaca ctctttttag
     2581 gagtaggctg tttctgtcgt ctagagcttc tagtttgttc atttgccatt cttgctttct
     2641 ccttcaatag ataatgttga taaatagaag tcaaccgcgt ccaaataagg aatccgcgga
     2701 gaaataccag ttttgatctc tattcctagt tcttcgatca aagataaagg tagtgatttg
     2761 cgtccttttt ccttttgggc gtcccagtaa gcaatcaaat gaacactatt caaataaaaa
     2821 cagcgcttca aagacgaaaa ccataacaag acaaagcaga ccccattttg ttcgaggcat
     2881 gctttcatat gttcgatttg atgggcatgg aagtttttaa agggaaaaga tgttttgttc
     2941 ttggtttctt ttgcttcaaa gtccaagtaa aagcctcgat agacgccatt atagtctgta
     3001 gttgaggctt gggtaaagta ggcttccttg atcacagcag cacttctctt aggatagtct
     3061 actttcacga tttggatagg ggtaggcttt ttatggataa ccgccaaccc gcgggaaaga
     3121 taatatttat tactttcatt gattgcttct tcaaagcgca tcccccgatt gccgaattca
     3181 gtgatctgat ttcttttttc ttgaattttt tcgacgggag aggcattctt ataataaggt
     3241 tggccatttg gatagcgcaa taccatcttt aatcacactc ctaggccaca ttatagcaaa
     3301 aaagcggata gaaagaaaag gaatctgaga tggataagtc aaaagtaatc tatgtgtctg
     3361 ggtatcgaag tttcgaactc ggtgttttta aagaaaatga tcctaaaatc caagttgtta
     3421 aaaatgtttt aaaaaaagaa ctccatcagt tagcagaaga agggctggaa tgggtacttg
     3481 tcagtgggaa tttgggcgtc gaactatggg ctgtgcaaat agtagcggaa ttgaaagaag
     3541 aatatccgga actatctctt ggtattttgt atcctttttc tgattttggt aataactgga
     3601 atgaagggaa ccaagaaaaa aaacaattag cagaacaatt agcagattat gtcgaagctg
     3661 ttagccatca accctaccaa tcacctagtc agctaaagaa tcacactcgt tttatacttg
     3721 agcatacagt gggctgtctg ctgatctatg atgaagaatt ccctggaaaa acgaagtatt
     3781 tttttgaaga tgcacagaat taccaagcag atcatcccta cgaaattcgg ctgatcagta
     3841 tggacgatct tcaaaatttt agtgaataat cgatattgtg tttgatttat caactttttt
     3901 cctatacaat gaaaaaggta gaaatgttat tacgtaaatg aaaactagag gtgtaaatat
     3961 ggcgaatttg gtttatagtc ctaaagacat tttacaacaa gaattcaaaa caaaaatgcg
     4021 cggctatgat cccgttgaag ttgatgaatt cttagacaat atcatcaaag attatgaagc
     4081 ttacagcaaa gaactccttg ctttacaaga agaaaacgat cgattgagtg caaaagtagc
     4141 tcaattatct aaaactcaag gagcagctca aacacgcgtg caacaaacag aggcaccaaa
     4201 gagcgcagca gtgaccaatt ttgatatctt aaaacgcttg tcaaatcttg aacgtgaagt
     4261 atttggtaaa aagcttgatc aacaagcaag tgcagtcaaa ccagcacaac ctaatccaaa
     4321 taactacaca aatgcagaca caagcttaga tgataatgaa aaaacacgtc aattttaatt
     4381 tttgattaat gaatttgcgg ttttcgggta atcgcggtct gacttgatca ggctgaggaa
     4441 agtccatgct cgcacaggct gtgatgcctg tagtgttcgt gcttagcgaa accataagct
     4501 aaggtactct ttggagtaac ggcaggaaaa caagctaagg cttcggctat gcttaagtat
     4561 ccttgaaagt gccacagtga cgaagcggct ggggaaaccc atccggtgga acgcggtaaa
     4621 cccctcgagc gagcaaccca aacaatggta ggggcgcttt ttgtctggaa gaagaacagg
     4681 acaaaaaggc agtataactg cagatagatg gttaccgctt tgttttttct ccctggaaaa
     4741 gcaaagtaca gaacatggct tatagaaaac cgcaaacatc agggaattag gtcgtgacaa
     4801 aagtaatgcg ttataagaaa caagaaaaac atttgaaagc tatttttcaa tacttcagat
     4861 gaatcgttct tattacaaaa caaacttttg tatcaccggt tattcggaat ttgagtaaaa
     4921 gtgtggggta ggactgatgt cccacctcct ttttttataa ataaggagat tgtgtatgga
     4981 aaaaaacagg aagttcaacc tcgtcgcaac tgcggcaagc gggctagaag cactggttgg
     5041 taaagaatta agagatcttg gaatcgaatg ccaagttgag aacggacgtg cacgttttga
     5101 aggaacaatg gaaacgatcg ctacagctaa tttatggtta cgtacagcag atcgagtaaa
     5161 aatcgtggtt ggggaattcg atgcgtatac gtttgatgaa ttgtttgaaa acgtaaaagc
     5221 attgccttgg gaagattttc ttccactaga tgcagctttt cctgtagctg gaaaatcgat
     5281 caagtcgaaa ctatatagca cacctgattg ccaagcgatc acaaaaaaag caatcgttga
     5341 acgtctccgt acctattatc accgtccagc ttctgtacca ctgacggaaa ctggcgcaca
     5401 ttttcagttg gaagtagctt tgctgaaaga ccatgtgatg gtaactctgg atacgacagg
     5461 accaagttta ttcaaacgtg gttatcgctt agaaaaaggt ggagcaccat tgaaagaaaa
     5521 catggcagca gcacttatta tgttgacaaa ttggcgaaaa gaccgtccat tttatgatcc
     5581 tgtatgtggt tcaggtacgc tttgtattga agcagcctta ataggccata atatcgcacc
     5641 aggattcaat cgtgagtttg cttgcgaatc ttgggattgg ttcagtcaag aaatcatgga
     5701 aaatgttcga tcagcagccg aagaaaaagc agattatgat attgaattag atattactgg
     5761 ttcagatatc aatggacgga tgattgaaat tgccaaagcc aatgcagaag aaatcggatt
     5821 aggctcttcg attaccttta agcaacaagc agtcaaagac ttcaaaacgg ataaagaata
     5881 cggggtcatc gtggctaatc cgccttacgg agaacgtcta ggagaagaag aaactgttcg
     5941 gaaattatat aaagaaatgg gcgatgtatt ccgtccatta aaaacatgga gtaaatatat
     6001 cttgactagc gacttagcgt ttgaggaatt ttacggacaa aaagcaacca aaaaaagaaa
     6061 attgtacaat ggtgcattgc gtacggatct attccagtat tggggaactc gtccgcctag
     6121 aaaaaaagag aaagacgaat aatcttacta attgattcat tcaattggat gtttggagga
     6181 atagggaatg aaagaaaaag agtttttaca agaagtaaaa gaaatcaatc ttctacaaca
     6241 agctttagga atcctcgatt gggatacaca aacaggtatg cctgaaaaag ctagtgcata
     6301 ccgaagtgaa gtagacagct atctttacag catttatttt tcaaaaaaaa tcggtccaaa
     6361 aatcaaagaa gccattcgct atttttctga acatccggaa gaattgtcgg aagttggaac
     6421 tgccgttttt gccaaagtaa aagaagagta tgatttggaa catcgcgtac cggaagatct
     6481 tatgagaaaa tatacagctg ccgtttcttc agcacacgga caatggcaaa aagctagaga
     6541 aacacaacaa ttttctgatt ttcaacacgc attgaatcaa aatattgaat taacaaaaca
     6601 gttgatacct tactggagaa aagatgaaaa gacgaattat gatgttttgc tgaatcaata
     6661 cgaacccggg atgactgtcg aaatccttga taaggtattt gatcagctta aagaaggaat
     6721 tctttcgatc cgccgtacgc tccaagaaaa agggactgaa cctgaaacag actttttaaa
     6781 tcggagaatg acgaaaaaac aacagaagag atttgtagtg aaagtaatcg aacaacttgg
     6841 ttatgatttt agtcgtggtc gtttagatga cactgtccac ccatttatga ctggaattaa
     6901 tccgaatgat gcacgaatca cgacacgatg gaacgaaaca gatttcaaaa tggctgtttt
     6961 tggagtgatc cacgaggctg gtcatggtat gtatgaacaa gacatcgatg ataaatatgc
     7021 gtatacaccg gtttatgaag gtacatcaat gggtatccat gaatcacaat cgttgtttaa
     7081 cgagatcatt attggaagta gtcgcagctt ctggaaaaag caatatcctt tctttcaaga
     7141 atgtgcagaa ggaacattcg atgatattcc ttttgacaaa ttttatcgtt cattgaacca
     7201 tacaaaatca agtttggttc gtatcgaggc agacagtttg acttaccctc tccatatcat
     7261 cattcgttat gaaatcgaaa aaatgatttt caatgaaggg gtagatgttg ctgaactacc
     7321 tgaaatatgg aatcaaaaat atgaagagta tctgggaata cgaccaacaa atgatgtgga
     7381 aggcatccta caagacgttc attggtcagg tgcaagcttt ggttattttc cctcttatgc
     7441 attagggtac atgtatgcgg cacaattact tcattctttg cagcaagaaa tagatgtgga
     7501 aaatgttcta gcgtcagacg attattctcc aatcaaaaac tggatgtcag agcatatcca
     7561 tcaatatggt gcatctagaa aaccaaatca attaatcatg gatgcaacac atgaaccgct
     7621 taatccgcaa tacctgatcg actatatgaa agacctgtat tttaatgtat atcaggtaaa
     7681 ataaaataca atttacaaag atagctgaaa ttcatctaat ctatagatgt tttcagctat
     7741 ttacttaaga aagaagtgga acgaatggat aaattacaag tgatcgctga ttatagtatg
     7801 gaccaattag cgcatgatca aacaggacat gggagcgatc ataccaaaag agttgtcaag
     7861 ttagctgaaa aaattttgga gacggaaccg caggcagacc gctttgtgac gcttgcagca
     7921 gcttacctac atgatacgat tgatgataaa gtcgttaaaa atgagaacga ggcaaagcaa
     7981 cagcttcgtg tattccttcg ctctttgcca atagtagagg aacagatctc gatgattttt
     8041 tccattatcg aaaatatgtc cttttctaaa aatctctctg aagcggccga actatctctg
     8101 gaaggtaaga tcgttcaaga cgctgatcgt atcgaagcat taggagcaat cggcattttg
     8161 cgtactgctt atttcggcgg tggacatggg cacccgattt ttgatcctga actgtatccg
     8221 caaacattca aggacaaaga aaattaccga aaaggcacga cagtgatcaa tcatttttat
     8281 gaaaaactgt tcttgctgtc tgataaaatg aatactgatt acggatatga agaagccaaa
     8341 cgaagagaaa ccttcatgcg tgaattctta gaagaatttt ttctggaatg gtctattgac
     8401 gatgaaaatt ttactccaga caattggatc aaaaataaca aaaaatgatg gaggttgtga
     8461 cagaagtggt cagctccaag atatttcgga tcattttccg taaaagttac ttctggaaca
     8521 ctgtttacca agaggtcgtg agcctgccgt tcagctccga gtcacaagga acaatttcaa
     8581 aaaatagctt ctcgtgtttg atgaatattg taacttgtga ttcggaattg gcaggagaac
     8641 accgtttatt cggaagaata ggactgtgac aagacttttg tcacagtcct tttttcgaat
     8701 tcaacaagta aaacttttaa gaaatattca gagagtaaaa ttaaaaattg ctgataaacg
     8761 aagatggaaa ttttatgtat ttttattggt ctatgcaata aataaattaa atattcagaa
     8821 tacaattgat tttaacgaaa gggcttctat caatggttgt gttgtattta tctgcccagg
     8881 tagagtaaaa tagatcatta tctaaaaggt aattgttcat actatataag tcatttgaaa
     8941 gtaaaaagat actgtaaata aaaaatgtgg ttaaataatt ctcataatca gtgtaagcgt
     9001 tatcaaaaat atagcatact tgctttgtag atgaatgaca cgatatatgt ttggtatgtt
     9061 ctgcaagaca gatgaatatg gaggtggaga caatgtcttc caaagaacta acaatgtatt
     9121 tagagaaaga attggatcaa ctgaaagaaa aggggttata caatacgatc gacatactag
     9181 aaagtgaaaa tggtgcttgt atcatcgtag acggaaaaaa gatgatcaat ctagcatcca
     9241 acaattatct tggatttgcc aatcgcgaag aattgaaaaa agcatgtatt gaagcaaccg
     9301 aaacttatgg tgtaggagca ggagcagtcc gaacgatcaa tggttcgctg aaaatccatc
     9361 agcaattaga agaaaaaatc gcagaattca aagggacaga agcagcaatc gcctttcaat
     9421 ctggattcaa ctgcaatatg ggcgctatct ctgcagtaat gacaaaagaa gatgctatct
     9481 tatcggatga attaaaccat gcatctatta ttgacggttg ccgcttatcc ggagcaaaaa
     9541 tcatccgaat taaacatcaa gatatgaaag accttgagaa aaaagcaaag gaagcgatag
     9601 aaagtaagaa atataaaaaa atcatgtata tcacagatgg tgttttctcc atggatggag
     9661 acattgcacg attgccggaa attattccga ttgttgaaaa atatggactg atcacctacg
     9721 tcgatgatgc gcacggctca ggcgtaactg gaaaaggtgc aggaaccgtc aagcattttg
     9781 gattaagcga caaaatcgat atgcaaatgg gcacgttatc gaaagctata ggtgttgttg
     9841 ggggttatgt agctggatcg aaaacactaa tcgactggtt gaaagctaga agtcggccat
     9901 tcctattttc tacttcgcta actccaggtg cagctgcagc tgcattggct tcgattactt
     9961 tgatgcaaga acatccggaa cttgtcgaaa aagtttggga aaatgctaat tattttaaag
    10021 aagaattgaa aaaagtaggc tataacatcg gtatgtctga aacacctatt actccagtca
    10081 ttttgggaga cgaaaaagtg actcagactt tctctaagaa gctgatcgaa catgggattt
    10141 atgctaaacc aatcgtttat ccaactgtgc ctcttgggac aggcaggatc agaaatatgc
    10201 cgactgcgga acatacgagg gaaatgcttg atgaagcagt agcagtttat caaaaaatag
    10261 gaaaagaaat ggaaattatt taactaaatt tagttttttc gtctatttaa aattagagaa
    10321 catgttcacg catatattgt taatcatctg aagatagtag taaaaaagat cagggaggat
    10381 catcatgaaa aaagtattgg tgacaggatg tttaggacaa attggttcag agttgatcac
    10441 acggctacgt caagaactag gcagtgaaaa tgtcattgcg acagatatac gcatgcctga
    10501 agtcattgag aaaaacgata tctttgagat cctagatgta atggattacg aaaaaatgcg
    10561 cagcatagca gaacaatatc aagtggacac attgattcat ttggctgctc ttttgtcagc
    10621 tgttgcagaa gcaaaaccac aattagcctg gaatttgaac gtgactgggc tggtcaatgc
    10681 attggaagtc gctagagaat tccagctgaa attttttacg ccaagttcaa tcggcgcgtt
    10741 tggtccaaat actccaaaag atcatacccc acaagatact cttcagcgtc ctacaaccat
    10801 gtatggggta acgaaggtag ctggagaact actatgtgat tattattatg tgaaatatgg
    10861 cgtagatact cgtggtgtcc gtttccctgg attgatttct tatgcaacct tgccaggagg
    10921 cggtacgaca gactacgcag tcgatattta ttattcagca atcaaagaag gaaagtacac
    10981 tagtttcatt gatcaaggta catcaatgga tatgatgtat atgccagatg cgatcgatgc
    11041 cattataaaa ctcatgaatg cagatcctga tcgattggtt catcgaaatg ccttcaatat
    11101 atcggcgatg tcttttgacc cggaagagat caaagcatct attcaaaaaa tcataccaga
    11161 ttttgaaatg gattatgatg tggatcctgt acgtcagtca atagcagaat cttggccaaa
    11221 ttcactagat gtttcttgtg cgcaagagga atggggcttt tcgccgaaat acgacttaga
    11281 tgtgatgaca aaggatatgt tggaaaaact gaaagaaaaa ttggtagaga cggtataaaa
    11341 caagaaccac atccaaaaag ttttttatac ttttgggatt ggctatggta tgaaagggga
    11401 caagggatgg aagaatatca agccacgcca gtaaaagaag taaaaataga aaatgaatta
    11461 aaacgtacga tgggattttt tactgcttta tcgactgtta tgggcacagt gatcggtgca
    11521 ggggtatttt ttaaagccgc aagcgtagca gaagttacag gatccgccag tttgcatatg
    11581 ttttcttggt ttctcggtgg tatgatttct gtctgtgcag gactaacagg agctgaactg
    11641 gcagctgcta ttccagaaac aggagggatg attaaatata tcgaacggat ttacggaaat
    11701 acagcagctt ttttattggg ctgggcgcaa gtcgtgattt attttccagc aaatgtagca
    11761 gccttgtcga tcattttcgg tacacagttc gtcaatctat ttggtctttc ccagtcaatg
    11821 attgttcccg tagctgttac agctgctgta tcgatcctgt tgatcaattt tttaggttct
    11881 aaagctggag gggcttttca gtcgattacg cttgtctgca agcttattcc tttgtttgtg
    11941 atcgtcattt tcggtttgtt ccgacaagaa ggagtagact tccaactgtt tccgatccaa
    12001 gcaggagaaa atctttcatt tttctcagcc ttgggagcgg gcttgctagc aacgatgttc
    12061 gcatacgacg gttggataca cgtaggaaat atttcgggag aattgaaaaa gccggcgaaa
    12121 gatttgccaa aagcaatttc tttaggaatc atcggaatca tgatcgttta tttattagta
    12181 aatgcggtgt tcttaagaac cgcatctatc gacggagtcg caggaaacag caatgcggca
    12241 agtgacgttg caaaaatgat ttttggcggc ttcggtggaa gactggtcac agttggtatc
    12301 ttgatttctg tttatggaac catcaatggc tataccctta ctggaatgcg tctcccttat
    12361 gttatggcaa aagaaaacaa cctgcctttt agcaagctct ttgctaaact tcatgataaa
    12421 actaaagttc ctgtagccgc aggtatctta gagctagtca tcgctattgg catgatgatg
    12481 atcggtggtt ttgatacact aacggatatg ctgatctttg tcatttggat tttttatacg
    12541 atggtctttg ttggtgtcat tcttcttcgt aaaaaagaac ctgatttgtt tcgaccatac
    12601 aaagttccaa tgtatccgtt tattccacta gtagcaatca ttggagggac ttttatcgta
    12661 tcaagcacgc ttatcactca gaccttttta gcttcgatgg ggatagtatt gacacttgct
    12721 ggtatcccga tttatctcta tttaaaacgt agatagctaa aacattcgaa ttttattaga
    12781 actgcacttc tctgccaaac aaaatatttt atttaaatga ggagtacgtg gaagaaatgt
    12841 caaaagtact cgttaatttt gatcacgtaa atgtcgtttt gtctacgatg aaatagcaaa
    12901 gtaaaagaca gaaatcgggc tcaaccgatt tctgtctttt actttgattt gcgtaactga
    12961 actattcggc gtacgccgcg ataaatattt aataaaacca agcaaccaga aatgaaaatg
    13021 ggaagccaaa tcagatagac agggtagcca agcactttag acaaagcatg tggagtcatt
    13081 ttatccattt caagaagtgc ttgctgccaa gtctgaagat tgaagactag catgaaaccc
    13141 gaaataatca ggagaataaa tcctaaataa tgaattgtag ttctttttct tacataagcg
    13201 atcgtagcat aaaggaacag taaaaaaata ggtacgccaa aaacaaagta catattcagc
    13261 atctcgacac cccctttgtg tttatcttac tttactttga aagaaatgag aaataatagc
    13321 ctcgttactt gcttgaaaca gcaaaggact gctaagataa gagagataca aagaatcaag
    13381 gaggagtcat atggcaagtt atatcgaacc gattcgtttt gcgctgattg tttttccatt
    13441 tttagcctta gcgatctcat ctgttttttt tatttatgaa tatcgaaagt atggaacatt
    13501 tctagtaaca agggcagtta ttttgtattc tttcgttttt tatttattgt gtgcgtattt
    13561 tttggtgatc ttaccacttc cggcaaagga gacggttgcc caaatgacag ggccaaagat
    13621 ggagctgcat ttatttgctt catggcaaca tttcagagac aacacggtat tggtcttgtc
    13681 tgaacctagt acctacctgc cggcaatgaa gcagagtgta tttttggaac cagcttttaa
    13741 tgttttatta ctcataccat ttggcattta tttaagatat tatttccggc tttcttttat
    13801 gaaaacgatg attgctagtt tctgcctttc tctatttttt gaattgacgc agctaagtgg
    13861 actttatttt atctacccaa gaccataccg cttatttgat gtgaatgatt tagtgacaaa
    13921 tacattgggt ggaatcattg gttttgtcgt tacgccgtta tttacattta tgcttcctac
    13981 aagaagacga atggatgaac tttcttatga aaaaggaaag actgtctcat tgactcgacg
    14041 cttggtcgct tatttgatcg actggacatt tttatctctt attaatttgg tagcggcgat
    14101 tttctttaaa attgcgttcg attcaggggc agttacttct catatctggt ggcttttatt
    14161 agaagtaggg gtatacttta tgattgtacc gtttataacc aatggatgga cacttggcaa
    14221 aaaaattgtg cgtatccgtt tggtggaaga agaccgcgaa cgaatttctt taaaagcatt
    14281 gatgatccgc tacgggtatc tgtactttct tttttatggt ttaagctggt tggggcagtt
    14341 gcttcagcaa atcatgaatt cgacaacgaa tacggtgttg cagatgagtg cagtattcat
    14401 ttctctcggt gtcttgttac tacaaggtat ttttatttta aatatcttgc tatcattttt
    14461 gagaaaaaaa cggattctgt tttatgaaaa agctagtcac acatatactg ttagcacgat
    14521 tggccaaaca aaggatgatg attctcgaaa ttagctgagc aaaaacgctt tgtctgtgtt
    14581 ataatgccaa agtcgtgata atttaaagag aagaggtgca cgaatgaata attttgatat
    14641 attccgcttc aaaccgttta tcaatgaagc gttaaccgca aagggcttca aacagcccac
    14701 agaggtccag gaacgtttga ttcctttaat tgcaaaagga aaaaatatca ttgggcaatc
    14761 tcaaactggt acaggaaaaa cacatacgtt tttgttgcct ttgtttgaaa aaatcaatgc
    14821 agaaaaagag gaagtacaag ttgttattac agctccaagt agggaactag ctgcacagat
    14881 ctatgaagca gctacacaaa tcgctaaatt ttctacaccg gagatccgtg tctcgaattt
    14941 tgtcggcggt acagacaaac agcgtcaatt aaatcgtctg catcaccaac agccacaggt
    15001 tgtagtaggc acgcctggta gaatattaga catgatgaat gaacaagcgc tgaagattca
    15061 caccgcattt gcatttgttg tcgatgaagc ggatatgaca ttggatatgg gttttttaaa
    15121 tgaagtagac caaattgcaa gtcgattacc agaaaagctg cagtttttag ttttttctgc
    15181 cactattcca gaaaaacttc gtccattctt acgaaaatac atggaaaatc caatcattga
    15241 agaaatcaaa ccaaaagcag ttatttctga agacattgaa aattggctga tttcaacaaa
    15301 aggtaaagat gagaatcaaa tgatttatca actgctgaca attggtcatc cttatttagc
    15361 aatcgtattt gccaatacaa aacagcatgt ggatgaaatt gccgattact tgaaaaacca
    15421 aggcttgaaa gtagcaaaga ttcatggaga catcacccca agagagagaa aacgagtgat
    15481 gcgacaagta cagaatctgg agtatcaata tgttgtagcg actgatttag ctgctagagg
    15541 gatcgatatc gaaggtgtct ctcatgttat taatgcagaa attccagaag atctagactt
    15601 ctttatccat cgtgtcggac gtactggacg taatggctta aaaggaacgg ccattacgtt
    15661 atattcgcca aatgatgagc aagccattga tgcgatagaa aaattgggcg tctcatttga
    15721 accaaaagaa atcaaaaacg gggaaatcgt caaaacatac gatcgtaata gacgtgccaa
    15781 acgagaaaaa tcaaaagatg tattagatcc cacgctgatt ggtcttgtga agaagaaaaa
    15841 gaaaaagatc aagcctggtt ataagaaaaa aatcgattgg gcaattgcag aaaaaaataa
    15901 aaaagaacgc aaaatcgaac gcagacagca aagccgtact gctcgtaaaa ataagaaaaa
    15961 ttcacataaa taaaaaacaa gaggttgtga caaaaggcgt gtcacaatct cttgttttta
    16021 agctagactt tttttcattc cgatacaaag tacttcatct tcaataaaag gttctgtgca
    16081 agtatgatac ccttgttttt cataaaaatc tacagccgtc atttcagcta ctacataact
    16141 agtctgacaa ccttctgaaa ccgcccgttt ttcagcagct tcaagcaact gaccgcctaa
    16201 cccttttcct cggtattctt ttgctacgca aaatcgttcc ggcttgaagc aagtaggtgt
    16261 tttcttttca taacgcaaag tagctactgg tcttttttga tcaagaaata gataataggt
    16321 ttgttgcggg atatcttttc cgtcgaattc tagttctgca ggaatccctt gttctaatac
    16381 aaaaacttga tatctcaaaa agcagctagc tgcgcgtatc cattcatccg ctccaactat
    16441 ttttttcatc atattcctcc ttgtcatgaa tagcagagat gtattgattc ctttattctt
    16501 atcgtaccaa aaatcgtgga gtatgaccaa aaggaaatga aaataaaaat tgagatattg
    16561 acagtgatca atagaatgcg tatactaatt aagtacgaaa aggatatgat ttgtttttta
    16621 agactcacag aaaattcttt cttggctgaa aaaagaatag tcactaatca aattgctccc
    16681 tttttctatt ttggtagaaa taccgacgca gctcagcgtt atcgagttta agaggtaatg
    16741 attacacatc attgcaatca aggtggtacc gcgagttttc gtccttggct gtgatggtgt
    16801 ttttttgtat ttaatttata gaaagcaggt aatgacatga aacaattaag tagtgcacaa
    16861 gttcgacaaa tgtttcttga tttcttccaa tctaaaggac atacgattga acctagtgcg
    16921 tctttgatcc ccgtgaacga tccaacctta ttgtggatca actctggtgt tgcaacattg
    16981 aaaaaatatt ttgatggttc agttgtgcca gaaaatccaa gaatcacaaa tgcccaaaaa
    17041 tctattcgaa caaatgatat cgaaaatgtc ggtaaaacag cacggcacca tacgatgttt
    17101 gagatgctgg gtaatttttc gattggtgac tatttcaaaa atgaagccat ccactgggca
    17161 tgggaatttt tgactgatcc aaaatggatg gcattcgatc cagaaaaact ttatgtaaca
    17221 gtttatccaa aagatacaga agcaaaacgc atctggcatg aagaagttgg cctgccacta
    17281 gatcacatta ttgatgtaga agacaacttc tgggatatcg gtgcaggccc atgtggtccg
    17341 gatacggaaa ttttctatga ccgtggagaa gcttataacg atttagcaga agacgatcct
    17401 gaaaactatc caggaggaga aaatgaacgt tatttagaaa tctggaactt agtattttca
    17461 gaattcaacc ataagcctga taacacattt gaaccacttc ctcataaaaa cattgatacg
    17521 gggatgggat tggaacgcat ggtctcaatc gttcaagatg cgtcaacgaa ctttgagaca
    17581 gacttgttta tgcctattat ccacgccgta gaaaagctaa gcaaacaagt aaaatatggt
    17641 gaaaacccag taacagatat ttcttacaaa gtaatcgcag atcatattcg cgccctttca
    17701 tttgctattg gcgacggtgc attgccatcg aatgaaggga gagggtatgt cttacgccgt
    17761 ttgcttcgtc gtgctgtcat gcatgggaaa aaattaggta ttgaacaagc attcttatat
    17821 cgtttagtcc ctgttgttgg ggaaatcatg gtaagttatt atcctgaagt acttcaacaa
    17881 aaagaattta ttgaaaaagt tatccgtaca gaagaagaac gtttcaatga aacaatcaat
    17941 gaaggtttgg aaatcttaaa tcaagtgatc caagaagtaa aaaacgaaaa cgggacgact
    18001 ctagcaggaa aagacatctt caaactatac gacacatatg gcttcccggt agaattaacg
    18061 gaagaggtgg cagaagatga aggactgaaa gttgatcatg ctggttttga aaaagaaatg
    18121 gatgcacaac gcgaacgtgc tcgctctgcc cgcagtactg aaaaatcaat gggggtacaa
    18181 tctgctttat tgacagatat caaagtagaa agtaaattca ttggatacac agaacaagaa
    18241 tcagatagtc aattattggt catcgttaaa ggtgagtctc ttttgaatga agtcgcagaa
    18301 ggtgaagctc aaatgatctt tgatcaaaca cctttctatg cagaaatggg tgggcaagta
    18361 gcggatcaag gatggatcat gaatgaagta ggcgaaatcg tagcagttgt tacagatgtt
    18421 caaaaagcgc ctaatggtca atttatgcac accgtagaag taaaagcacc aatgagtgaa
    18481 ggacaaacct atcatctatt gatcgatcaa tcgatgcgca atcggatcat caaaaaccat
    18541 acagcaaccc atctactgca caaggcattg aaagaagtac ttggcgagca tgcaaatcaa
    18601 gctggttctt tagtcgctcc tggacattta cgattcgact ttacccactt tggtcaaatc
    18661 acttcagatg aattagctga aatggaaaga atcgtcaacg aaaaaatctg ggaagctatc
    18721 ccagttgaaa caattgaaac agatatcgat acagccaaaa acatgggtgc aatggctttg
    18781 tttggtgaaa aatacggaca tgacgttcga gtagtaaata ttggcggctg gtcaatcgaa
    18841 ctgtgcggag gcacacatgt agcaaataca gaaaacatcg gaatcttcaa aatcgtttct
    18901 gaatcaggaa ttggtgcagg cgtgcgacga atcgaggctg taacaagtaa agaagcgtac
    18961 gaactacttg aagaagaaga aaaacaacta aaacaagtag caacaattgt taaatcacct
    19021 caattgaaag aagttgtttc aaaagcggaa caattgcaac agcaagtaag agagctacaa
    19081 aaggaaaatg aacaattagc tagcaagtta gcgaatcaac aagctggtga tgtattcaaa
    19141 gatccagaaa cgatcaatgg aaaaacaatc attgcagcac aagtaaaagt gaaagatatg
    19201 aatcagctgc gtcaattagc tgaccaatgg aaacaaaaaa atatttcaga tattcttgtt
    19261 ttagcaaacg aacaagaagg aaaagtcagt ctgctagcgg caatgacgaa agaatcaaat
    19321 gaagctggac taaaagctgg cgacttgatt aaagcaatcg caccgaaagt aggcggtggc
    19381 ggtggtggac gtccagacat ggcacaagct ggcgggaaaa atcctgctgg tattccagat
    19441 gcacttaaag ctgcgaaaga atgggttgcg caacaatagg gcgtttacgt ttgacagaaa
    19501 aacaaaagtt caaacattga acgaaacatg attatggggg cgaagacaaa caagtcttgc
    19561 cctcatactt ttttcaattg gatggcgcat ctaatcaaat ggtcggattc ggatggtctt
    19621 tagttgacga aagtgacaga aatttttacg attagtataa ataaaaaggg gtgaaaaaat
    19681 gaatagtaga aataagacct ttcgactagt cttacgtgcg atcttgttag caatcatcat
    19741 cgtacaagcg atggttcctt ggctggggtt tattccatta ggattcatta gtttaacaat
    19801 tatccatatc acagtaatta ttgctgcagt agtattaggc ccaaaagatg gcatggtgat
    19861 tggcttgttt tggggaatcg ctacaattgt tcgtgcctat gccatgccaa caacgccatt
    19921 tgatacatta gtgttcacga atccgattat ctcggtagtt cccagagtac ttgttggctt
    19981 agtagcaggc ttggttttcc actggattta tcaaaaaaat aaatcaataa cgatcagctc
    20041 agcctttgca ggggtactgg gttcattggt caatacagtc ttagtgttag gattcatggg
    20101 attattttat acaggtgcaa cggcagatgc ctatggtgta gactctgctc ttttatttaa
    20161 aaccttagca ggggttgcag caatcaatgg gataccagaa gcaataggtg cgggaatcat
    20221 aacaccatta ctagcaaaag cgttatttgc agcaacaccg ttgaaaccag aataatgacc
    20281 atcttttaat agtaatagga cgcacgatag atagaaaaag gaagagagga gaggaaaaca
    20341 atcgtcctct ccatcttttt acctgagcaa caagcaaact agaaggggta aaaaacttgg
    20401 ctaagaaaca agaagaaaaa aagaaacaaa taccgcaaca gcggacaaaa aaaggagtac
    20461 tagcgggtat cttaggattg gttacgaatc tgatcttgtt tgtctctaag ctaatgatcg
    20521 ggcttttttc aggaagtgtc tccattatgg ctgatgcagt gaacagtcta tcagatactg
    20581 cttcatccat attgactttg atcggattta aaattgctgc aaagccagca gatcaggaac
    20641 atccttatgg acacgaacgc ttcgaatata tcagtgggtt atttgtttcg atcatcatta
    20701 cttatgtagg atttcaattt ttagagtctt ctgctgaaaa aatcatccat ccagaaaatg
    20761 tctctttaac acccatcgtc ttttttgtcc taatcatttc catctttttg aaatttgcac
    20821 aaggcaaaat gtatcaggtt attgcgcaat ccattgattc tgaaacattg agagctacag
    20881 caacagatag ttataatgat gtctttacta cactggctgt attgctttca gcagcaatcg
    20941 aatgggtaac tggatggcgt attgatggat atataggttt tgctttagca ttatatatcc
    21001 tttatagtgg attgacgatg cttcgaaatt ttgtatacga actattagga agtcgcccaa
    21061 cgccagcaga gattgaagaa atggaagagc atttaaatgc ttgcgatgat atacttggct
    21121 atcatgattt attggtgcat aactatggac ccaataaacg ctttgcatca gtccacatcg
    21181 aagtagatga atcaatgaat ttgaatgtgg ctcataagat tattgatctg atagaaaaag
    21241 actttaaaaa gtctttggat gtggaacttg tctgtcactt ggatccaatg cctgttgata
    21301 acgaaaaata ctataatatc ttgcggcagt taaagcaaat tcttcttgaa acaggtacag
    21361 ggcttaatat tcatgatttt agagtaattc atcaaggaaa aacgctgcaa tttgatgtag
    21421 tggtccctga gaaattccag tttgttgata cagaactaga aagaatctta cgtagtcaaa
    21481 tagaagaaaa aatcggagat taccaattgg aaattacgtt tgatcacaat tatcttttat
    21541 attaaatgag aaactataaa aagactcctt aaagttttta atttaaagag tctttttttc
    21601 ttttaattgt ttatggagag cctgacgtgc aagattatct gctcctttat ttttgttttc
    21661 gggcaaccat tgcaggatca ataaagaaaa ttcggataga agttcttgga ttctttgaag
    21721 gtagggaagg aagagctcgt tcttcgttgc gtttttgtcg accgtagaaa ctagtgtttt
    21781 gctgtctgaa aaacagaaaa tcgtttcatt ctgtaagtcg tttttcttta gaaattctaa
    21841 agtttttaaa aagacttcga actctgcttg atggtttgtc cctatagaaa gcgggataga
    21901 tagctgttct tgtttacctt gatagacaat caagattcca cctccactag gaccagggtt
    21961 gccttttgta gaggcatcaa tgtatacttt taacataaaa agagattctc ctttttgttc
    22021 aaactaatgt tatggtaaga ttatatcatt actgaatcgg tgagaggtta gagaatgaaa
    22081 aaaaatattc gatggcagcc tgaactggca catacgatca tctattggtc attgaccttg
    22141 atcattctgt tttttagtct gattctttct ttagaaaaca caggtcctta ttggaaaagc
    22201 aattttgttt taggtatttt tcttgttttt ctatttctag gtttgcggcg ttcttttatt
    22261 ttaaaatcaa actctgtgat tatacgttat gctgcttttt ggaaagaccg agaaatatct
    22321 gtcggacaga ttcgatcttt gacgccaact aatactgggg tgcaaatgat gttgcaagat
    22381 gagaaagccc catctgtttt catgatgcga aaaaaagatc aagtagtcct ttttgatcat
    22441 tttgaaaaaa acggacccaa atcagaatca attccttatc cttatacgaa aaaaagaaaa
    22501 agctaatttt aaaaatcaga acctaaaaaa acgaattttt acaggaaaag aaaagagtga
    22561 gacgtacggt tcatcccgtt tagcgtctca ctctatttgt ttatctattc atgcatacta
    22621 gtatcggtag tctgaacgtt gcctttcttg acatgggcag cttgtgttcc tcgattccct
    22681 tcgatgatct caaattcgac cacttgattt tcttccaaag ttttgaatcc ctcttcttca
    22741 atcgcagtaa aatgtacaaa aatttcttct gtgtcatcat aactgataaa gccgtatcct
    22801 tttttgttgt caaaccattt tacaattcct gtggtcaaag cactcacgac ctttctaaag
    22861 tctgatcata gccttcatta taaagagtat ttataagtac gtcaaataaa ttaaaaacct
    22921 gtaattttat gttttttttc
//
LOCUS       DAYYCY010000025        22911 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000025 DAYYCY010000000
VERSION     DAYYCY010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22911
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.025"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..530)
                     /locus_tag="ACHAZB_000823"
     CDS             complement(<1..530)
                     /locus_tag="ACHAZB_000823"
                     /EC_number="3.2.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase family
                     protein"
                     /protein_id="HFM8613514.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKT"
     gene            1014..1241
                     /locus_tag="ACHAZB_000824"
     CDS             1014..1241
                     /locus_tag="ACHAZB_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFM8613515.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            1257..2423
                     /gene="hemW"
                     /locus_tag="ACHAZB_000825"
     CDS             1257..2423
                     /gene="hemW"
                     /locus_tag="ACHAZB_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFM8613516.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            2657..3700
                     /gene="hrcA"
                     /locus_tag="ACHAZB_000826"
     CDS             2657..3700
                     /gene="hrcA"
                     /locus_tag="ACHAZB_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HFM8613517.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            3794..4357
                     /gene="grpE"
                     /locus_tag="ACHAZB_000827"
     CDS             3794..4357
                     /gene="grpE"
                     /locus_tag="ACHAZB_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFM8613518.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            4408..6237
                     /gene="dnaK"
                     /locus_tag="ACHAZB_000828"
     CDS             4408..6237
                     /gene="dnaK"
                     /locus_tag="ACHAZB_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFM8613519.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            6388..7554
                     /gene="dnaJ"
                     /locus_tag="ACHAZB_000829"
     CDS             6388..7554
                     /gene="dnaJ"
                     /locus_tag="ACHAZB_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFM8613520.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(7636..8265)
                     /locus_tag="ACHAZB_000830"
     CDS             complement(7636..8265)
                     /locus_tag="ACHAZB_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8613521.1"
                     /translation="MGQLSDNQQLCQERINPLLELLERVFSYYGQALLTVHRQQIIIL
                     VNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSFLHAKQVCEWIAF
                     HQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLFKKTLACFSKNNG
                     SIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLLVQT"
     gene            8277..8450
                     /locus_tag="ACHAZB_000831"
     CDS             8277..8450
                     /locus_tag="ACHAZB_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8613522.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            complement(8495..9232)
                     /locus_tag="ACHAZB_000832"
     CDS             complement(8495..9232)
                     /locus_tag="ACHAZB_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM8613523.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(9234..10736)
                     /locus_tag="ACHAZB_000833"
     CDS             complement(9234..10736)
                     /locus_tag="ACHAZB_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFM8613524.1"
                     /translation="MKRKHLFHSFVFLMTGLVTFAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            10995..12989
                     /gene="uvrB"
                     /locus_tag="ACHAZB_000834"
     CDS             10995..12989
                     /gene="uvrB"
                     /locus_tag="ACHAZB_000834"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFM8613525.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            12999..15815
                     /gene="uvrA"
                     /locus_tag="ACHAZB_000835"
     CDS             12999..15815
                     /gene="uvrA"
                     /locus_tag="ACHAZB_000835"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFM8613526.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            16078..17343
                     /locus_tag="ACHAZB_000836"
     CDS             16078..17343
                     /locus_tag="ACHAZB_000836"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HFM8613527.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            17347..18078
                     /locus_tag="ACHAZB_000837"
     CDS             17347..18078
                     /locus_tag="ACHAZB_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HFM8613528.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            18213..19097
                     /gene="rapZ"
                     /locus_tag="ACHAZB_000838"
     CDS             18213..19097
                     /gene="rapZ"
                     /locus_tag="ACHAZB_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFM8613529.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            19094..20092
                     /gene="yvcK"
                     /locus_tag="ACHAZB_000839"
     CDS             19094..20092
                     /gene="yvcK"
                     /locus_tag="ACHAZB_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFM8613530.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            20126..21061
                     /gene="whiA"
                     /locus_tag="ACHAZB_000840"
     CDS             20126..21061
                     /gene="whiA"
                     /locus_tag="ACHAZB_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HFM8613531.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            21604..22254
                     /locus_tag="ACHAZB_000841"
     CDS             21604..22254
                     /locus_tag="ACHAZB_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8613532.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            22441..>22911
                     /locus_tag="ACHAZB_000842"
     CDS             22441..>22911
                     /locus_tag="ACHAZB_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HFM8613533.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIIL"
BASE COUNT         7537 a         3913 c         4925 g         6536 t
ORIGIN      
        1 atcgtttttt ctcttatttc ttctgtttca aaatggactg cttgaagaat caagtctgtg
       61 aattctatag gagctgttcc attcgcagta atcaaattac gatctttcac agcttgttgt
      121 tctataaaat cttctttatt ctgataattt tccgattcta accaaggaaa ttgcgcattt
      181 ccggtgtgtt tgtaaccttg caataatccg ctctgagcta aataatcaac tgccccacaa
      241 atagcagcta ctggtacgtc tgtttttaac aatttatcga ttatttgata taaatgatga
      301 ttttttatgt cccatgaatt gccaccgatc ataataaaaa ggtcaacttt ttcaggtatt
      361 tgttcgatta agtaatcgat ttttgttttt aatcctccaa tggaagtgac ctctgattgg
      421 atagaagcag ttttgttttg ccatttctca ttttggttta gtgtgctagt aatataagca
      481 ccttcccaat cggcatattc ctctaataga aacgtaatcg cagttttcat tttcaagctc
      541 tcctttacta tctataacaa ggacaacagt tttaacctac agacattcat taaaattggg
      601 tagcgacagc gaaaacaaca attcttaagt tttagacaga gtatacagat agtcaactta
      661 attaaatacg aactaaataa aaatgacagg taatcatttt tgtttaccag cattataaca
      721 ttttgcatac atggatgaca aaagaaccaa ttcccacttg cttgagtaca atattaaaag
      781 aaactggaag gcaaaaagta attatagaaa cgaaaaataa atcatatcgg gcactcagac
      841 atgaaaacaa ccctagagat atataactag gtaaccagtt taatgaaaga aaaaatcatc
      901 caagaagtcg attcttggat tttttagtgg atttgttcgc caaatacgtt tagtatgcgt
      961 atatgagagg ttgtaagcaa agcgttgtgg tctgagcact taaggaggaa tctgtgaaaa
     1021 tagcttttca tatttttgca gattttgact taatgccgaa ggtcagcttt gcgattaccg
     1081 tttatcgaga ggctgtaaaa gcaccgtgta gctccgatga caataagcaa gcatttttag
     1141 agctaggttt ccttgattct aaatatgcgg cttatttcaa tgagttggtg cttgaacgcg
     1201 attttaagaa ggtcgaaaac aggcacgact tttcgacgta aggagaaaga atttcaatga
     1261 tagaaacaat aaatcccgca gaaaacaagg tttctgcgta cattcatatc ccattttgcg
     1321 agcatatctg ctattactgt gattttaata aagttttttt agaagggcag ccagtagatg
     1381 agtatgtgga aatgctgctg aatgaaatga aaataatgct agaaagtcat ccgacagcga
     1441 atttagaaac actttacgta gggggcggga caccaacttc tttatctgct aaacaacttg
     1501 atcgactgtt atcaggtgct agagagatcc tgccttttca aaatggtaat gagtttactg
     1561 tagaagcgaa tccaggtgat ttgacccgtg aaaaacttca agtaatgaaa aactatggcg
     1621 tcaatcgttt atccatggga gtgcagacat ttgataatcg cttattgaaa aaaattggac
     1681 gtaaacacac cgcagaagat gtgtatcaaa ctatgcaatt tttagaagca gaaaatttta
     1741 ccaatgtaag catcgatttg atttatgcat taccgggaca aacactagaa ggatatctgg
     1801 atactttgga aaaagctttg gctttggatc tgcctcacta ttctttgtac tcattgatcc
     1861 tcgaaaacaa aacaatgttc atgaactggg tacgacaagg aagactcgaa ctgcctgatc
     1921 aggaaacgga aaccagaatg tttgaagaga cgattgaagc aatggaaaag catggacgtc
     1981 accaatatga aataagtaac tttgggctct ccggacatga atctcaacat aacctcatgt
     2041 actggaacaa tgaccactat tttgggtttg gagctggtgc tagcggttat cttggagata
     2101 ttcgttaccg caacaaagga ccaatccaac actatttacg cccattacga gcaaatgaac
     2161 gcccggtatt ggaagaagaa aaattgacac gaaaaaatca aatagaagaa gaaatgttct
     2221 taggactaag aaaaaaaaca gggattacta aaaaacattt ctatgatcgc tatcaaaaaa
     2281 caattgaatc ggtgtacggt gatgtgctaa gggaattaga gcaagaaggg ttacttgtta
     2341 atgaagaaga ccgtgtttat ttgacaccaa gagggacctt tttggggaac aatgtgtttg
     2401 agcgcttttt gtttgaaaaa tagtttaaag aatatttgtt ttttatctga atttaaataa
     2461 aaaaagttca aaaatacaat aaaggactat tgaaacgttg atataactgg atttacatca
     2521 attatttaga attagcactc ttgtgtttag agtgctaaaa aatggagtct ttctcttgac
     2581 aggagaaggg cttcttgcta tatttataag tggattagca cttagaatat atgagtgcta
     2641 atgagaggtg aggaagatgt taacaaagcg acaaaacgac attttacgtc tgatcatcca
     2701 acactatacg agtagtggcg tgcccgttgg ttccaaaaca ttgatgtctg agggagtcaa
     2761 ggccagctca gcaacgattc gcaatgatat gaaagcatta gaagatgaag gattgctgtt
     2821 gaagactcac tcttcttctg ggcggattcc atctgcttta ggctatcgtt attacgtgga
     2881 tcatttgtta aaacctgctc gcgtagcaga agatgatttg ctgctgattc gtcagtctct
     2941 aggcagagaa tttcatgaaa tcaatgagat tattaagcaa tctgctgaga tattatctga
     3001 gctgacaagc tatacgactt tttcattggg acctgaaata aaagaccgcc gactgactgg
     3061 ctttcgtatc gtacccttga ataatcgaca gattattgct atcgtcgtaa cagatatggg
     3121 gaatgtagaa agccaagtgt tcactcttcc cgaatatatg ggaagtcagg aactagaaaa
     3181 aatggtacag attgtaaacg accgtctagt cggagatcct ctgatgacgg tgtaccataa
     3241 acttcgtacg gagattccga tgattcttca taaatatttc caaacgacag aagggatgtc
     3301 tcagttattt gacaccgttc ttggtcatgt ttttgaagat aaggtttatg tcagtggaca
     3361 aatgaatctc ttggattatg agccccgtca agatcttgat cagttcaaat cgatgttttc
     3421 ttttatgaaa gactcagacg gacttacaca aatgatcact ccgatggata gcaatattca
     3481 tatcaagatt ggtgctgaat taggcaatga tttattgcag aatatgagta tgattcaagc
     3541 aagttacgag atagatggtc atggaagagg tacaatagcc ttgttagggc ctactagtat
     3601 gccttattct cggatgttcg gtctagtcga tgtttatcga agagaactag cagctaaact
     3661 tgctgattac taccgttcgc ttgacttgtc aaactcgtaa tgtgaataac gaagctattt
     3721 gcagctgatc gttctctctt tcttagtaag agcaatcaaa cagctgactg aataatgaaa
     3781 ggaagactgt atcgtgaaag aaaatcaaga agaactagat aaagaaatga cagatgctca
     3841 gccagaaccg gaaatcgatg tcgaagctgc agaagattcg ggtatttcag aagctgaagc
     3901 ggaagaattc gaaacagcga aattaaaagc tgagttagaa gaaatggaag ataaatactt
     3961 gcgggctcgt gctgaaatcg caaatatggc aaatcgagga aaaaatgagc gtgagcaatt
     4021 acaaaaatac cgttcacaag atttagccaa aaaattactt ccatcaattg acaacctgga
     4081 acgtgcatta gccacagaag tgtcagatga tcaaggggca ggtttgaaaa aaggcgtgga
     4141 aatggtcttg gaaagtttgc gtaacgcact ggaagaagaa ggaattgaaa aaatccctgc
     4201 taaaggagaa gcctttgatc ctaatcttca tcaagcagta caaactgttc cagcgactga
     4261 agatacacca gccgatacga ttgtagaagt actacaagaa ggctacaaat tacatgatcg
     4321 cgtgttaaga ccaaccatgg tcattgttgc acaataattg tgcggatata aaacttacac
     4381 tttcaattaa agaaggagac gttaattatg agtaaaatta tcggtattga cttaggtacg
     4441 actaactctg cagtagcagt attggaagga aacgaagcaa aaatcatcgc taacccagaa
     4501 ggaaaccgta caacaccttc tgttgtatca ttcaaaaatg gagaaatcca agtaggggaa
     4561 gttgcaaaac gtcaagctgt gacaaaccca aatacgattt catcaatcaa acgccatatc
     4621 ggtgaagcag gttacaaagt agatgtagaa ggtaaatcat atacaccaca agaaatctca
     4681 gcaatgatcc ttcaatatat caaaggattc gcagaagact atttaggtga aaaagtcgaa
     4741 aaagcagtta tcactgtgcc agcttacttt aatgatgcac aacgtcaagc aacaaaagat
     4801 gctggtaaga tcgctggtct agaagtagaa cgtatcgtca atgaaccaac tgcagctgct
     4861 cttgcctatg gtttagataa aacagatcgc gacgaaaaaa tcttagtatt cgaccttggt
     4921 ggcggtactt ttgacgtatc tatccttgaa ctaggagacg gtgtctttga tgtattgtca
     4981 acagctggcg acaaccatct aggtggggac gactttgata acaaaatcat cgactacatg
     5041 gtagcagaat tcaaaaaaga aaacagcatt gacttgtctc aagacaaaat ggctttacaa
     5101 cgcttgaaag acgctgctga aaaagcgaaa aaagatttat ctggcgtatc aagcacacaa
     5161 atcagcttgc catttatcac agcaggagaa tctggaccat tgcatttgga aatgacattg
     5221 acacgtgcaa aatttgatga attaacagct gacttagtag aacgtacaaa agtaccagta
     5281 cgtcaagcgc taaaagatgc aggtttatca caatcagaaa tcgacgaagt tattttagtc
     5341 ggtggatcta ctcgtatccc tgcagttgta gaagcagtac gtaaagaaac aggaaaagaa
     5401 ccaaacaaat cagttaaccc tgatgaagta gttgctatgg gtgctgctat ccaaggtggc
     5461 gtgatcactg gtgatgtgaa agatgttgtt ttacttgacg ttacaccatt gtcattaggt
     5521 atcgaaacaa tgggtggagt gttcactaaa ttgattgacc gcaatacaac gattccaaca
     5581 agtaaatcac aagtattctc aactgcggca gataatcaac cagcagtaga catccatgta
     5641 ttacaaggtg aacgcccaat ggctgctgac aacaaaactc taggaagatt ccaattgaca
     5701 gatattccag cagctcctcg tggtattcca caaatcgaag taacattcga tattgataaa
     5761 aacgggattg taaatgtatc tgcgaaagat ctaggtacac aaaaagaaca aaaaatcacg
     5821 atcaaatctt cttcaggtct aacagacgaa gaaatcgaac gtatggtaaa agatgcagaa
     5881 gcaaatgcag aagcagataa agctcgtaaa gaggaagttg acctacgtaa tgatgtagat
     5941 gcattgctat tctctgtgga caaaacattg aaagaattag aaggcaaagt ggacgaagaa
     6001 gaagtgaaaa aagcagaaac agctcgcgat gaattgaaag ccgctgttga agcaaacaac
     6061 atcgaagaaa tgaaaactaa acgcgatgca ttgaacgaaa tcgttcaaaa cttgactgtt
     6121 aaactatacg aacaagcggc acaacaacaa gctcaagaaa acccagaagc agctcaaggc
     6181 ggagcagatg atgtcgttga tgctgatttt gaagaagtcg atggcgacga taaataatca
     6241 gttcactgaa tttcgagaga aggccaaagt tgctactttg gctttttctt ttgaaaaata
     6301 gtttgaagta tcggtttttg tttcaaacaa aatatgatat gataacaagc gacgtgaaaa
     6361 tgatgagaac caataggagg aaagcctatg gcaacaaaac gtgattatta tgaagtctta
     6421 gggctctcaa aaggagcatc agaagatgaa ataaagaaag cttatcgtaa gctttcgaaa
     6481 aaataccatc ctgacatcaa taaagaacct gatgcggaag aaaaattcaa agaagtatca
     6541 gaagcatatg agattttaag tgacccgcaa aaacgcgctg catatgacca atacgggcat
     6601 gcaggcacag atccaaatta cggtgctggt ggagctggtg gcggatttgg tggattcggc
     6661 ggcttctctg gtggcggctt cggtggtttt gaagatatct tcgactcttt ctttggagga
     6721 ggcggtcggt cagtcgatcc aaatgcgcct cgtcaaggtg ctgatttgca atatacggtc
     6781 gacttgtctt ttgaagaagc gatttttggt gtagaaaaag aaatcaagta taatcgggaa
     6841 gaaatctgtc atacttgtgg tgggaacggt gcaaaaccag gcacacaacc aactacttgt
     6901 cataaatgtc atggtgcagg tacgattaat gtagaacgcc aaacaccact tggacgagtg
     6961 atgagccgtc aaacttgtga tgtttgtcat gggacaggga aagaaatcaa agaaccttgt
     7021 ccaacctgtc atggctcagg acatgagaag aaagcacatt cagttaaagt gaatgtacca
     7081 gctggtgtag aagaaggaca acaaatgcgt ttagctggtc aaggagaagc tggagaaaac
     7141 ggcggaccat ttggtgattt gtatgtggtc ttccgtgtag aagaaagtga tatctttgat
     7201 cgtgaaggtt ctgaaattta ctatgaattg cctttaagtt ttgtacaagc agctttgggg
     7261 gatgaagtcc aagtaccaac tgtccatgga gatgtgaaat tgaagatccc tgctggaaca
     7321 cagacaggta cgaacttccg tttacgtgga aaaggcgctc cgaagttacg tggtggtgca
     7381 acaggcgacc aacatgtcaa agtaaaactg atcacaccga aaaatctaaa tgaacaacaa
     7441 agagaagcat tacgtgcatt cgctgaagct ggcggcatca aagttgaaga acagcaagaa
     7501 gacggtttct tcgataagtt taaagatgct tttggcggga aaaagcggaa gtagtttttc
     7561 tagtaaaaac gctaagtcat taataattaa aaaccagacg aacaatcata tgttcgcctg
     7621 gtttttttgt agttgtcatg tttgtaccaa caaaaaagcc agctttagaa tatgataatc
     7681 gtcatagttt ctaggggtat aaccagtgag agaatggaac ttgttcaatc gatattgcaa
     7741 ggtgttttta tgaataaata attcttctga gcaatttttg atgcttccgt tatttttact
     7801 gaagcatgcc aaagtcttct tgaacaaatg tacttcttct tctgtcaggc ttttcaaaat
     7861 acgttttaca tataatgttc gttgatctga cgggattgct ggtaaaacga tcccgaggtc
     7921 taaatcttca aaaaagcgga tctcgttcac agattgatgg aaagctatcc attcgcaaac
     7981 ttgtttggca tgaaggaaag attgcggtgt ctcttgctct gtataacaaa gcgacccaat
     8041 tccaaagttt agctgtaact gatacatttt attaaacttt gtctggattt tatcaagcaa
     8101 tgatagcaat gaggctctgg aaatccgatt gactaggata atgatttgtt gtctatgtac
     8161 agtgagcaaa gcctgtccgt aataagagaa gaccctttcg agcaactcca ataaaggatt
     8221 gatccgttct tgacacaatt gctgattgtc agaaagttgt cccacaacta cataatatgg
     8281 agaagaaaaa tcaagctcaa ttaagtgagg gtggtatcga ctatgtactt gatatcatca
     8341 atgaaccgat ggtactttct tatgcgatgg aacaggtaga gcgattgctt gtttgtgcag
     8401 gaaaacaagc gatgcggctg atacgattag gtgagaaaat aagaagataa aaatacacgg
     8461 tcccttcatg gcgaccgtgc atttttggtt tttattaaat atttaatact ttattcaaga
     8521 aatcttttgt ccgttcattt tgcggatttt caaagatttg ttgaggcgtt ccatcttcta
     8581 aaaagtttcc gtcgtcaatg aacatgacgc gattggccac ttctttagcg aaccccattt
     8641 cgtgcgtaac gataaccatc gtcatacctt gttctgctaa ctttttcatg acgttcaaga
     8701 catcaccgac catttcagga tcgagtgcag aagtaggctc atcaaaaagc atgatgtcag
     8761 ggttcatcgc tagagcacga gcaatagcca cacgctgttt ttgtccacca gaaagcattt
     8821 caggatacat atctttttta tcttctagac caactgtctg cagtaatttg attgcttttt
     8881 tctctgcttc ttctctagat tctttcttca ggtccatcgg tgctaaaatg atgttttcca
     8941 aaatagaaag atgagggaat aggttaaagt gttggaatac cattccgata tgttgtctca
     9001 ccaagttgat atctgtgctt ttatccatca agtgggcgcc gtcaataata atttcaccgt
     9061 cagaaggttc ttctaaacga ttcaagcaac gcaagaatgt actttttcct gaacctgaag
     9121 ggccaatgat acagacgaca tctccttcgt tgatggatac attgatgtcg tttaatactg
     9181 tgttatcgcc gtattttttg actaaatgct caactaagat tttttctgcc atcttacttc
     9241 accttctttt ctaaaacttt tgctaatttt gtcaatgctg tgatcaaaat caagtacatg
     9301 atcgcaatga tcaagtaaac atacgtactt tgtgttgtac gtgcaacaat gatcttacca
     9361 gtttgcagca attcgactac accgattgcg gaaatgatcg ttgtgtcttt taatgagata
     9421 acgaactgat tgacgaatga tgggatcatg atgcgtatcg cttgcggcaa aatgattttt
     9481 tgcattgtcc gattataaga aagacctaaa cttctggaag cttccatttg tccgacagga
     9541 acggcgttga tcccgcctcg aacaatctct gcgatataag cacttgcgtt aagagtcaag
     9601 gtgataacac cagcaaggaa gtcaggaatc gtaaatccaa cgataccagg caacccaaag
     9661 aaaataaaga acgcaagaac catcattgga attcctcgga tgatatcgac ataaatacta
     9721 gcaattgtgc gaagagcgcg tattggtgct acactgaata atccaaaaat cacaccgatt
     9781 actatagcca aagcaaagga gatcaaagtt aggagaattg ttctccaaag tcctttcaac
     9841 aatgcttgat agttgttttg gataagtcca agaaatgttg attcatcagc agtggaagac
     9901 tcactggaag tttctgaacc atccgctaca tattctttaa taatcttatc gtattcgcct
     9961 gttcttttta attctttcaa tccttcattg aacatattaa gaagctcagg attttgtcct
    10021 tttttaacgg caaatgcata ttttccgcca gattcttttt tgatcggtgt ttccaattct
    10081 tgtccttgtt tgatcgcata accgattacc ggataatcat ccatcattgc atcgattgcg
    10141 ccaactttta acccgtcgta caattgatca gcagcatcaa aatatttgat agaatagcca
    10201 tattcagatt catggtcgct taaaaattca gcactttcgg taccaacttt tgctccgact
    10261 gtttttcctt tcaaatcttc ataagacttg atcgaagtat ctcctttttt gacagctaat
    10321 tggatcccgc tatcaaagta agaatcagaa aaatcatagc ttttctttcg ttcatctgtt
    10381 acagtcattc cggcaatcat tccgtctgct tgtcctgatt ccaaagcttg caaagcagca
    10441 gagaaaccac ggtagtccat tcggatgtta aatccttgca gtttggcgat gttttccatc
    10501 aattttacat cgatgccttc gtactcgccc tttgcgtttt gaaattcaaa tggagcaaaa
    10561 gcagagtcac tggcaattac gtatacgtct tttttgggct ctattttttg cattgtctcg
    10621 cttgtgcttg actctgccgt tgtagtgttg tcagcttgtg caacatgacc aaacgcaaat
    10681 gtcacaagtc ctgtcattaa gaaaacaaat gagtgaaata aatgctttct tttcataatc
    10741 aacctcctaa atttccaata ttttattatt ataggcgaaa agtgctgaat cagacaagac
    10801 tgtaagctcg aaaaaatgaa aaaaatgcga gtttctaaca ttttatgtta taaaacataa
    10861 catgtataat aagataaaaa agtggcgaaa gtatgttcgg tgtggtataa ttttttagta
    10921 aggaatgcct ttgctttcct tttcttttta ttggtcgtaa aatgaaaagg aatgaatgaa
    10981 aaggaggacg aagaatgatt gagagagata cctcccgaca ctttgagctt gtctcgaaat
    11041 accaaccggc tggtgatcag cctgaagcga ttaaccaatt agtggatggc gtagtcggcg
    11101 ggaaaaaagc acaaatctta ttaggtgcga ctggaacagg gaaaacatat acgatttcca
    11161 atcttattga gaaagtgaat aaaccaacac tgattattgc ccataacaaa acattggctg
    11221 gtcagctgta cggggaattc aaggaatttt tccctaacaa tgctgttgaa tattttgttt
    11281 cttattatga ttattatcag cctgaagctt atgtgccttc aagcgatacg tatatcgaaa
    11341 aagattcaag tgtaaatgat gagatcgaca aattacgaca ttcggctact agttcattat
    11401 tagaaagaaa tgatgtgatc gtcattgcat ccgtttcatg tatttttgga ttaggctcgc
    11461 cttttgagta ccaaaaacaa gtagtttcga ttcgacaggg agcagaacta gatcgcaatc
    11521 agttgattcg tgacttagtc agtatccaat tcgaaagaaa tgatatagat tttcagcgcg
    11581 gtcgttttcg agtgcgcggt gatgtcgtgg agatttttcc ggcttcaaga gatgaacgtg
    11641 ccttacgtgt ggagtttttt ggagatgagg tcgaacgtat ccgagaagtc aatgcactga
    11701 caggtgaagt tttaggagaa acggaacacg tagcaatctt cccggcgact cactttgtga
    11761 cgaatgatga acatatggag catgccgtag cgaatatcaa agcggaatta gaacagcgtt
    11821 taactgtctt gcgaaatgaa aacaaacttc tggaagctca acgtttagaa cagcgaacca
    11881 attacgatat tgaaatgatg ttagaaatgg gctacacgtc gggaattgaa aattattctc
    11941 ggcatatgga cggacgtaaa gaaggtgaac cgccttatac gctattggat ttctttcctg
    12001 aagatttttt gatcgtggcg gacgaatcgc acgtaaccat gcctcaaatc agaggaatgt
    12061 ataatggtga ccgagcgaga aaacagatgc tagtagatta cggtttccgt ttgccatctg
    12121 ctttagacaa tcggccactt cggttagaag agtttgaaaa acatgtcaat cagatcattt
    12181 atgtctctgc gacgccagga ccatatgaac atgaacaaac agataccgtt atccaacaaa
    12241 tcatccgtcc gacagggctg cttgacccag tgatcgaagt tcgtccaatc atgggacaaa
    12301 tcgatgatct agtaggcgaa ataaatgaac gagtcgaaaa agaccagcgg gtgtttgtaa
    12361 cgacattgac gaaaaaaatg gcagaagacc tcacagatta tttcaaagaa ttagggatca
    12421 aagtcaaata tttgcatagt gatatcaaaa cgctggaacg aacagaaatc attcgagatc
    12481 ttcgattagg tgaatttgat gtgttagttg gaatcaattt gcttcgtgaa ggattagacg
    12541 tacctgaagt ttcccttgtt gctatcttgg atgcggataa agaaggcttt ttacgtagtg
    12601 aacgttcctt agtccaaacg attggacgag ctgctcggaa tgaagaaggt aaagtaatca
    12661 tgtacgcaga caaagtaaca gattctatgc gtctagcgat ggatgaaacg tcaagacgtc
    12721 gtacgattca gcagaagtac aatgaagaac atgggatcgt tccaaaaacg atcattaaag
    12781 aaatccgaga tttgatttct attacgaaag aatcggaaga tgatacaaaa gaagcggtac
    12841 aagtctcgta cgaagaaatg acgaaggaag aaaaagacac gttattgatg aaattagaaa
    12901 aagaaatgaa agatgcggca aaagctttgg attttgaaac agccgcgaat gtgcgagata
    12961 tgattttaga attaaaagct tcaaagtagg aggacaacat ggctaacgat aaaattacga
    13021 ttcatggtgc acgtgcccat aacttgaaaa atatagatgt aacgattcca cgagataaat
    13081 tcgtggtcgt tacaggtttg tccggttctg gaaaaagttc cctagcattt gatacgctgt
    13141 acgctgaagg gcagcggaga tacgtagaga gcctttctgc ttatgctcgt cagtttttag
    13201 gacaaatgga taagccggac gtagatagca tcgacggatt gagtccggcg atttctattg
    13261 atcagaaaac aacaagtaaa aatcctcggt cgacagtagg tactgtgacg gaaatcaatg
    13321 actatttacg cttgttgttt gcacgtgtag gacatccgat ttgtcccaac gatcatatcg
    13381 agattaccag tcaatcagta gatcaaatgg cagatcaagt aatggctttg cctgagcgca
    13441 caaagatcca agtgttggca ccgatcgtgg taaagaaaaa aggacaacat aaaaaaatat
    13501 tcgaacgaat ccaaaaagag ggatacgtcc gcgttcgagt cgatggtgag atctatgatt
    13561 taagtgaagt gcctgagctg gagaaaaaca aaaaacatga tatcgcgatc gtcatcgacc
    13621 ggatcattgt gaaagaaggg attcgttctc gcctatttga ttctttagag gcggccctgc
    13681 gattagcaga cggctatgct ctggttgacg tgatcggtga agaagaaatg ttgtttagcg
    13741 aacactatgc ctgtccttat tgcggattta ctgttggcga actagaacca agactgttct
    13801 cattcaatgc gccttttgga gcttgtccag actgtgatgg tttaggtgtc aaattagaag
    13861 tcgatcaaga tttagtgatt ccagatagaa cgaagacact tagtcaaggt gctatcgttc
    13921 catggaatcc aatcagctct cagtattatc ctcaaatgtt agcgcaggct tgtgaaagtt
    13981 tcggtatcga tttagataca ccattcgaag aattaccgga agagcatcag gagatcatct
    14041 tgaatgggac agatgatctt ttccatttcc attatgaaaa tgattttggt ggcgttcgcg
    14101 atgtagaaac gactttcgaa ggggtattga agaatatcga gcgtcgttat catgatacga
    14161 acagtgattt cacgcgtgag caaatgcgtc tttatatgac agaattaact tgtcagacat
    14221 gtaaaggtta tcgattgaat ccccaagctc tatctgttca aatcaatgga acgaatatcg
    14281 ggcaaacaaa tgagttgtct attcaaaaag cagtagattt cttctcaaac ttaacgttat
    14341 ctgaacaaga gaaagtaatt gccaaaccga ttttaaaaga agtaaatgac agactttcat
    14401 tcttggaaaa tgtgggacta gactatctaa ctttgagtcg cgcttccgga acattatctg
    14461 ggggagaagc gcagcgaatc agattggcta cacaaattgg atcgaatctt tctggagttt
    14521 tgtatatttt agatgaacca tcgatcggat tgcatcaacg cgacaacgat cgactgattg
    14581 gctcattaaa gaaaatgcgt gatcttggca acaccctcgt tgtagtcgaa catgatgaag
    14641 atacgatgtg ggcagcagat tatttgattg acgtcggacc tggggccgga gaacaaggcg
    14701 gacaaatcgt ggctgcaggc actcctgaag aagtagctaa tgatgaaaat tctttgacag
    14761 ggaaatatct ttcagggaaa aaatcaatcc ctgtaccaaa agaacgccgt aaaggtaatg
    14821 ggaaagcaat caagatcact ggtgcttcag aaaacaatct gaaaaatatt agtgttgaat
    14881 ttccattagg agaatttgta gcagtcacag gtgtgtctgg ttctggaaag agtacattgg
    14941 tcaatagtat cttaaagaaa tcgttagcgc aaaaattaaa taagaattct gctaagccag
    15001 gtaaattcaa gacaatttcc ggctacgaaa gtatcgaaaa gatcatcgat atcgatcaaa
    15061 gcccaatcgg ccggacgccg agaagtaatc cagcgactta tacaagtgta tttgatgata
    15121 tccgtgggtt atttgctcaa acgaacgagg caaaaatgcg gggttataag aaagggcgtt
    15181 ttagtttcaa cgtaaaaggc ggtcgttgtg aagcttgtcg cggggatgga attattaaga
    15241 tcgaaatgca ctttttgcct gatgtctatg ttccttgtga agtatgtcat ggtaaacgat
    15301 ataattctga aacattagaa gtgcattaca aaggaaaaag cattgctgat attttggaaa
    15361 tgacagtaga ggatgctgta gaattcttca agcacattcc aaagattcat cgcaaactgc
    15421 aaacgattgt tgatgttggc ttaggttatg tgacgatggg gcaaccagca acgacattgt
    15481 ccggtggtga ggcacaacgg atgaaacttg ccagtgaatt gcacaaaatc tctaatggaa
    15541 agaatttcta tatactagat gaaccaacga caggacttca tagcgatgac atcgcccgct
    15601 tgttgcatgt attacaaaga ttagtagatg ctggtaacac agttttagtg attgaacaca
    15661 atctagatgt aatcaaaaca gcagattata tcattgattt aggaccagaa ggtggagaag
    15721 gtggaggaac gatccttgcg actggaacac cagaagaaat cattaacgta aaagaaagtt
    15781 atacaggtca ctatttgaaa aaaataatgg tataaagagg atagatagct gagatagtta
    15841 aaaaaacata aaaaaatgaa tttttgcttg ttttattaat atcagtgttc tagaatcatt
    15901 acatggtagt tctttctaca ctgattggta tagataaaaa cttcctataa gttcaatccg
    15961 ttcaaaaaag aattgagcaa tcggaggact ttcctttgat tgcttttttc tttatggtat
    16021 gatattcctt gtgtagttgg aaaagaaaaa caagagatta tgagtaagtg agagataatg
    16081 atgaacagta ttgaaaatga agaatttatc cctatattat taggaagcga catgaacgtt
    16141 tatggtatgg cacgttcttt taatgaagca tacggcaaaa tctgccaagc atatgcttcg
    16201 gatcagttag cccctacccg ctacagtaaa attgtcaatg tagaagttat tccaggattt
    16261 gataaagacc ctgtatttat cgagacgatg cttcgtttgg caaaagaaag atattctgat
    16321 aagagtaaaa aatatctttt gattgcttgt ggtgatggat atgcagaatt gatctcccaa
    16381 cataaacaag agctgtcaga atattttatc tgtccatata ttgattattc tttatttgaa
    16441 aggctgatca ataaagtaag cttttatgaa gtttgtgaag aatatgatct tccgtatcct
    16501 aaaacattga tcgtcagaga agaaatgtta gtaaatggac atttggaaca ggaattgcct
    16561 tttgaattcc cagttgcttt gaaacctgca aacagtgtgg agtatctttc tgtccaattt
    16621 gaaggaagaa aaaaagcttt tattttagag actagagaag agttcgattt aattttaggc
    16681 cggatatacg aagccggtta taaaagtgaa atgattgtac aagattttat cccgggtgat
    16741 gatagtaata tgcgtgtgct aaatgcgtat gtagatgaag atcatcaagt tcggatgatg
    16801 tgccttggtc atccactatt agaagatcct acaccagcat cgattggtaa ttacgttgtg
    16861 atcatgccag attataacga aaaaatctat caaaccatta aggcattttt ggaaaaaatc
    16921 gaatatacag gctttgccaa tttcgatatg aagtatgatc ctagagatgg agaatataaa
    16981 ttatttgaaa tcaatttgag acaaggtcgt agcagtttct ttgtgacatt gaacggcttg
    17041 aatctggctc gttttgtcac agaagatcga gtgttcaaca aaccctttgt tgaaacgacc
    17101 tatggaacga atcaatcaga taaagcaaga ttatggatgg gtgtgcccaa aaaaatcttt
    17161 ttagaatatg ctcgggaaaa tgaagacaaa aaactagctg agcaaatgat caaagaaaat
    17221 cgttacggta ctactgtatt ttatgaaaaa gaccgatcaa tcaagcgctg gttgttgatg
    17281 aaatatatgt tccataacta tattccaaga ttcaaaaagt attttcatgt gaaagaaggc
    17341 tagttcatgg agaatttttt cagtatttta ggcggaatgg gcacgatggc gacagaaagt
    17401 tttgttcgct tgatcaacca ccgaacgaaa gctacaaaag atcaagaata tttgaattat
    17461 gtcttattca atcatgcaac agttcctgat cgtacagctt atattttaga tcgatcagaa
    17521 gaaaatccaa tgccgtttct gctggatgat attgagaaac aaaatttatt gcgaccaaac
    17581 ttcattgtgt tgacttgtaa caccgcacac tacttttttg aagagttaca agcggcaacg
    17641 gatattccta tcttgcatat gccaagagaa gcagcaaatg aacttgtccg tcagcatacg
    17701 acagggagag tagcgattct aggaacagaa ggaagtatga aagctggaat ctatgaacgc
    17761 gaagtcaaaa atcttggttt cgaaacagtg attcctgata ctgcgcttca agaaaaaatc
    17821 aattacctga tctatcatga gatcaaggaa tcggattatt tgaatcaaga gctctattac
    17881 gaaatcttag aggaagcggt agagcgccta aactgtgaaa aagtcatcct aggttgcaca
    17941 gaattatctt tgatgcatga atttgctgaa gataatcatt acccagtgat cgatgctcaa
    18001 tctattttag cggatcgaac gatcgaacgt gcattagctg aaagaagcga ggcattggat
    18061 acagcatcgg aaaagtaaaa aaattggatt tggggcaaat atcctaaaag atatttggtc
    18121 caaatctttt tgtaaaagaa aaagaaaact ggtaatgata cgcccgggat gatataattt
    18181 tattatgcac aatgtgagag gggttcgtaa atatgacaga taatttgcaa ttagtcatca
    18241 taacaggtat gagtggtgca gggaaaacag tagctatcca aagttttgaa gatatgggct
    18301 atttttgtat cgataatatg ccgcctcgat tgattccaaa attctgggaa ttgatcaaag
    18361 aatcaggtaa agtcacaaaa atggctttag gagtggattt gcgttctcgt tccttctttg
    18421 aagaaatcca aaacatgctg atcgaaatcg aaaatacgaa cttcatcgac acttctatct
    18481 tatttttaga tgcttcagac gaagaactag tggctcgcta caaagaaaca agacgagcac
    18541 atccaatggc gatggacggt ttgatcactg aagggatacg taaagaacgg gcgatactag
    18601 acgacttgaa agcccaagct tccgtgatca ttgatacgac aacattgaca ccacgacaat
    18661 tacgtgaaaa aatcaatgag tcattcaaat caaagggaga ccaaggattt cggatcgaat
    18721 ttgtatcatt tggttttaaa tacgggttgc cgatcgattc ggatattgtg atggatgtgc
    18781 gttttttgcc caaccctcat tatatcccgg aactaagacc attgaccgga aaagatcagc
    18841 cagtctatga ttatgtgatg tcctttgatg aaacgcaatc attctaccat aaatttttgg
    18901 atcttctgga aactgtattg cctggataca ttaaagaagg aaaaagcagc ttgacagttg
    18961 caatcggctg tacaggcggt caacaccgtt ctgtggcatt gactgaacgt ttggcaaatg
    19021 ctttagggaa aaactataaa gtcaatgtga cacatcggga caaggataaa aggaaagaga
    19081 cggtgaaccg gtcgtgaaaa tgaaaaccta ccggataaga aaaccaaaga tcgtagtggt
    19141 cggcggtggc actggtctgc cagttatcct gaaaagttta cgaaatcaag gagcagatat
    19201 cacagcagta gtgactgttg cagatgatgg tggaagcagc ggtgcaatca gagaatcgat
    19261 cgctatggct cctcctggtg acttgagaaa tgtcttagtg gcgttatcgg atatgccaca
    19321 attctatgaa gatattttcc aataccgttt caaaaaggaa gatcagtttt tagccaatca
    19381 tacgatcggt aatctgatca ttgccgcagt ttccgagatg cggggaagca cttatgaggc
    19441 gattcaatta ttagcaaaaa tgatgcatgt agatggacat gtttatcctt cttcagaaac
    19501 tcctttgacc ttgcatgctg tttttaaaga cggaacgact gcagtaggag aatctaaaat
    19561 cgcgatcaac cgtaaaacca ttgaccgtgt gtatgtctgc aacacaaatg ataaaagtga
    19621 agccaaagcg gctcgaaaag tcgtttcagc aattatggac gccgacatgg tcgttttggg
    19681 acccggaagt ttattcacca gtatccttcc aaatttagtg ataccagaaa tcggcgaagc
    19741 aatgctcaag acaactgctg aaaccgtcta tatctgtaat atcatgaccc aaaaagggga
    19801 aacggaacat ttcacagatg ccgatcatat acgtgtttta cacaagcatt tgaatcagcc
    19861 ttttatcgat acagtattgg tcaatacgga aaaagtgcct gaagattata tggacccaga
    19921 gatctatgat gagtatctag tacaagtcgc acacgacttc aacggattga gaaatgaagg
    19981 atgtcgagtg atttccaccg actttttaga attgaaagat ggtggtgtct tccacgatgg
    20041 agacaaagta gtagaagaat tgttccgttt agttttcggt tcaaaatatt aagcaattta
    20101 ttgttggaga aaggaggaga ataggatgtc atttgctgca gatgtgaaga aagaactaac
    20161 tggtttggaa gttcatcgag aacatgcgaa agcggaatta gctgctttgc tgcgaatgaa
    20221 cggctcttta ggtattgtga accagcagtt tattttgaac gtccaaacag aaaatgctgc
    20281 aattgccaga cgtatctatt ctcttttgaa agatcattat caggtacgaa gtgagctact
    20341 cgttcgtaaa aaaatgaaat taaaaaagaa caatgtctat atcgtccggt tgaagcaagg
    20401 cacaaaagat gtgttgatgg atcttgatat catggatggt atgatgttca atggacacgt
    20461 ttcggacgag attatgggaa attcgcaaaa aatgcgttct tacttacgtg gtgcttttat
    20521 ggcctcaggt tcagtcaata atccggaaac gagtcgttat catttggaaa tattttctat
    20581 ttatgaacag cataaccaag acatttgcga tatgttaaat tattacggtc tacatgctcg
    20641 aacgcttgaa cggcgcaatg gctatatttc gtatttaaaa ggagcagaaa aaattgctga
    20701 tttcttaaca ttgattggtg caaccaactc aatgctccga tttgaagatg ttcgaatcgt
    20761 acgggatatg agaaattcgg tcaatcgtct agtcaattgt gagacagcaa atctgaacaa
    20821 gacgattgat gcggcttcta aacaaatcga aaacatcgaa ttgatcgaag caaaggtcgg
    20881 tctacatgcc cttcctgaaa aacttcagga aatcgcagaa cttcggcttc aacatccaga
    20941 ggtcagcttg aaagaattag gggaaatgat tccctcaggt gcaatatcaa aatcgggaat
    21001 taatcaccga attcgcaaaa taaatgaatt tgcggaaaaa ctgcgtgaag aagcagttta
    21061 atccaaagaa aaaactgata catgaagcag gagatgcgaa tgtatcagtt ttttttaaaa
    21121 gctatacttg cttttcttcc gtcaaactat aattccaact ttactggagt aacataatgt
    21181 tgaaaggctt ctattgtttt ttaatacaat cgtataaaaa gcggggagac aactgaatga
    21241 agattttagt tttatacata aacttttgct taatatagaa gtcctgatat agataggatc
    21301 aagtatttat ctataactgg agattaatta aaaactggaa ggaactgctc gatcaacctt
    21361 gagaactgtt tttcttactt ctggtcaaga ttctcgtggt tggtggataa ggatggttga
    21421 taagttcatt ggattagcta gtcgaaaaca accaggtgta attaatgtag aagttttaac
    21481 aaggggatgg agtgatttat tcagtgataa tcataagttc ataaatagta attgggatgt
    21541 atggttggta tacgttatac ctgaacttga actatcaaga tttggtttag aatttgagaa
    21601 tcgatgttta taaagggagg gcaaaaaatg tgctttttta caacttttac tatcattatg
    21661 attgcagtac tttttatttt tctagatata gcaaagcgaa atacagcatt tttattatat
    21721 cgggttttac ttcgagcagg tctcataaca tttatatcga tcgttggatt ttttctattc
    21781 acagttattg tatttatttg gagaactaca gcaccaccat tgccagagat tacatatgga
    21841 gaatttccgt ttagtcttga atatgaaata gatgaggaaa gaattttgat tgaagatacg
    21901 gtgattgcag aatttatagg aagttttaga gggaatgtga caactcaacc tagacgaagt
    21961 tggagtataa aacttttaaa taatagtgga gagttgaaag catttcatta tgaattctta
    22021 ataatagaaa gtgaagatgt aacaatcact ttaagtagtg gctctgctgg atattttatg
    22081 aatgatggtg gaagcaagcc acagctcaca gtccgtgatc gtattgcagg ttttatgcat
    22141 ttttctcact atgacttaga agaagctcgt aaaatacttg cagaatatgg gatcacatta
    22201 atcaattggg aatatgcgcc accgataaga aattttttca atgattatta ttagtcttat
    22261 aaaaaacaga atgtaaaatt tatgtgcgcg agtatttagt tttccatttt ttgtttgata
    22321 gatatatagc attgttgaag catgggatag tggtgccaat tttatcaatg aggttggtca
    22381 ggcagttaac ataattggtg atgctataaa tccatttaat tggaattgga ggaattaggt
    22441 gtggaaacag tgagattatg gatattcatg aaaaattttc gttcggcttt gagatatgtt
    22501 attctggaat caaaatcagg ttattatctg attgacacag atcactatac tttttgggga
    22561 ggctaccttt ttccgatttt taattggata atcccccata aggttatgaa aattaacttg
    22621 cctgcagaag aaataaatag attaatactc aacaaagatt tacaaaggaa aagagagaaa
    22681 gaacattcta atgttgcttg gataggtcca gcatttgcag tagtgacttc ttcaattgtc
    22741 ggtcctatgc tagagagtat ttatagtgat ttttctattg gaatacgtta tttatttgtt
    22801 actttaagta tagctgttct tttgtgcata aagggttatg ttagcaagaa gaataaagat
    22861 attttaacac taatcggtgt aaacaattta tctagtacta aaattatact g
//
LOCUS       DAYYCY010000026        22765 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000026 DAYYCY010000000
VERSION     DAYYCY010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22765
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.026"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..822)
                     /locus_tag="ACHAZB_000843"
     CDS             complement(<1..822)
                     /locus_tag="ACHAZB_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM8613534.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAI"
     gene            complement(1046..2986)
                     /gene="thrS"
                     /locus_tag="ACHAZB_000844"
     CDS             complement(1046..2986)
                     /gene="thrS"
                     /locus_tag="ACHAZB_000844"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFM8613535.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3323..3502)
                     /locus_tag="ACHAZB_000845"
     CDS             complement(3323..3502)
                     /locus_tag="ACHAZB_000845"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HFM8613536.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3549..5372)
                     /locus_tag="ACHAZB_000846"
     CDS             complement(3549..5372)
                     /locus_tag="ACHAZB_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM8613537.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5650..7929
                     /gene="helD"
                     /locus_tag="ACHAZB_000847"
     CDS             5650..7929
                     /gene="helD"
                     /locus_tag="ACHAZB_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase recycling motor HelD"
                     /protein_id="HFM8613538.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8022..8669)
                     /locus_tag="ACHAZB_000848"
     CDS             complement(8022..8669)
                     /locus_tag="ACHAZB_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8613539.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(8848..9567)
                     /locus_tag="ACHAZB_000849"
     CDS             complement(8848..9567)
                     /locus_tag="ACHAZB_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8613540.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9698..10849)
                     /locus_tag="ACHAZB_000850"
     CDS             complement(9698..10849)
                     /locus_tag="ACHAZB_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HFM8613541.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11066..11806)
                     /locus_tag="ACHAZB_000851"
     CDS             complement(11066..11806)
                     /locus_tag="ACHAZB_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8613542.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11810..12133)
                     /gene="rsfS"
                     /locus_tag="ACHAZB_000852"
     CDS             complement(11810..12133)
                     /gene="rsfS"
                     /locus_tag="ACHAZB_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFM8613543.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12190..12783)
                     /gene="yqeK"
                     /locus_tag="ACHAZB_000853"
     CDS             complement(12190..12783)
                     /gene="yqeK"
                     /locus_tag="ACHAZB_000853"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HFM8613544.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12767..13417)
                     /locus_tag="ACHAZB_000854"
     CDS             complement(12767..13417)
                     /locus_tag="ACHAZB_000854"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFM8613545.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13427..13738)
                     /gene="yhbY"
                     /locus_tag="ACHAZB_000855"
     CDS             complement(13427..13738)
                     /gene="yhbY"
                     /locus_tag="ACHAZB_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFM8613546.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13754..14863)
                     /gene="yqeH"
                     /locus_tag="ACHAZB_000856"
     CDS             complement(13754..14863)
                     /gene="yqeH"
                     /locus_tag="ACHAZB_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HFM8613547.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(14860..15387)
                     /locus_tag="ACHAZB_000857"
     CDS             complement(14860..15387)
                     /locus_tag="ACHAZB_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HFM8613548.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15567..16352)
                     /locus_tag="ACHAZB_000858"
     CDS             complement(15567..16352)
                     /locus_tag="ACHAZB_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFM8613549.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16345..17214)
                     /gene="accD"
                     /locus_tag="ACHAZB_000859"
     CDS             complement(16345..17214)
                     /gene="accD"
                     /locus_tag="ACHAZB_000859"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFM8613550.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17215..18594)
                     /locus_tag="ACHAZB_000860"
     CDS             complement(17215..18594)
                     /locus_tag="ACHAZB_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFM8613551.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18602..19021)
                     /gene="fabZ"
                     /locus_tag="ACHAZB_000861"
     CDS             complement(18602..19021)
                     /gene="fabZ"
                     /locus_tag="ACHAZB_000861"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFM8613552.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19046..19522)
                     /gene="accB"
                     /locus_tag="ACHAZB_000862"
     CDS             complement(19046..19522)
                     /gene="accB"
                     /locus_tag="ACHAZB_000862"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFM8613553.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19525..20760)
                     /gene="fabF"
                     /locus_tag="ACHAZB_000863"
     CDS             complement(19525..20760)
                     /gene="fabF"
                     /locus_tag="ACHAZB_000863"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFM8613554.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20779..21516)
                     /gene="fabG"
                     /locus_tag="ACHAZB_000864"
     CDS             complement(20779..21516)
                     /gene="fabG"
                     /locus_tag="ACHAZB_000864"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HFM8613555.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21528..22445)
                     /gene="fabD"
                     /locus_tag="ACHAZB_000865"
     CDS             complement(21528..22445)
                     /gene="fabD"
                     /locus_tag="ACHAZB_000865"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFM8613556.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22552..>22765)
                     /locus_tag="ACHAZB_000866"
     CDS             complement(22552..>22765)
                     /locus_tag="ACHAZB_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFM8613557.1"
                     /translation="KIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINEIEDE
                     FDVKIETEDGIKTVQDLVTYVEKQTA"
BASE COUNT         6230 a         5081 c         3983 g         7471 t
ORIGIN      
        1 gattgcttct cgtgtttttt tcactttttc gctaagcttt tcgtttttag ccatatagaa
       61 ataaaaaatg atatcgatcg tagcaaattg ggccaaaagt gaattagttg ccgcacttcg
      121 attcgtgatt tcttttggtc tagatgtttg tacgagtaca tcggctatct tgctaagcgg
      181 gttatttcca aattgcgtca acgcaatgat ttcgatattc atgcttttcg ccagctcggc
      241 taatgcgact acatctgcac ttctacctga gtttgaaaca agccaaagca ctttcttttt
      301 ctcattgctg acaagttgcg ggagcaagac gtggatatct ttttcaaaaa tgattggctt
      361 accgacacga gaccattttt gcagaatatc ttctactact aaagaagagg cgccaacccc
      421 atataaaaaa acagtctctg ccgattccaa acactctacg actttttgga tcgtttcttc
      481 ttctagaata tctactgttt cttttatcgc tacttgtgca ttgttaccaa gtttgttttt
      541 gatagagtaa aaggactcat ttgcttctat atcactaaaa ccttcatcga ttccagtctg
      601 tagttctgtt gaaatcgtga ttttgaagtc cgtcagactt tgaaaaccga tttttttgga
      661 aaaacgtaca actgtcggcg cactcaaccc ggaagcttct gctaattcat tagctgtcat
      721 tgtagggacc ttctcaatat taccgataat gtagttggcc aacttttttt ctgctgaaga
      781 ataatcttcc atcatacttt caatacgact aattacgctc ataagttcct ccaccctttt
      841 aatgatagtc tatgtaagta taccatatca gacccttatt cacggtcgta tactggataa
      901 aaaacaaaaa aacaatccgc ccaacagaat atcttatccc atcaatccca cagattctat
      961 tgagcatctg tgtctttgag gaatacctgt tatctattgt tcggattgct ttctatctac
     1021 tactttttta caggaaattt acctattagt tttctctgct gtaatttttg acttctgctt
     1081 ccatagcatc aataaagata ttcaaatctt ctacagctgt ttctttactt ccataacgac
     1141 gtacgttcac tgttgcatct tccaattcct tgtctccaac aactaattgg taagggatct
     1201 tttgtgtttg agaggcacgt atcttatagc ccattttttc attacgatca tcaacttcta
     1261 cacgcaaccc cttcatttgc aaacgttctt tgatttcata agcataatcg ccatgtgcat
     1321 caacagaaac agggatgatt gttgcttgta taggtgccaa ccatgttgga aaagcgcctt
     1381 tgtatacttc tgttaaataa gcgacaaaac gttccattgt agaaacaatt ccgcggtgga
     1441 taactactgg acgatgcgta ttttccccgt cttctccaac atatgtcaaa tcaaagcgct
     1501 ctggcaataa gaagtctaat tgtatagttg ataatgtttc ttctgtacca agagctgttt
     1561 tgacttgaac atctagtttt ggtccgtaaa aggctgcctc gccttctgct tcaaagtaat
     1621 ctagttctaa ttcatccata gcggctttca gcattgcttg ggctttttcc cacattgcgt
     1681 catcatcaaa atatttatct gtattgtttg gatcgcgata gctcaagcgg aaacgataat
     1741 ctgtaatatt gaagtcagca tatactgcca ccatcaattc aagcgtacgt ttaaattcat
     1801 ctttgatttg atcaggacga acaaatgtat gaccatcatt caacgtcatt tctcgtacac
     1861 gttgtaaacc tgacaaagca cctgattttt catagcgatg catcattcct aattcagcga
     1921 tacgaattgg taattcacga taagaatgga tcgtgttttt gtagaccatc atgtgatgag
     1981 ggcagttcat tgggcgaaga acaagcattt cgccatctcc catatccatt ggcgggaaca
     2041 tgtcttcttg ataatgatcc cagtgtccag atgttttgta caactcaaca tctcccatta
     2101 ttggtgtata tacatgttgg taacctagac taacttcttt gtctacgata taacgttcga
     2161 tcgtgcgtcg gattgttgcc ccttttggaa gccagaatgg caaaccagaa cctacttctg
     2221 gggaaaccat gaacaagtca agttcttttc ctagtttacg atggtctctt tcttttgctt
     2281 cttcacggag acggatatat tctttcaaag ctttcttgtc aaaaaaggct gtcccgtata
     2341 cacgttgcat catttgattg tttgagtttc cacgccaata agcacctgcc actgataata
     2401 atttaaatac ttgtatacgg ccagtggatg gaacatgtgg tccacgacac aaatcgatga
     2461 attcgccttg ttgataagca gtaatcactt cttcttctgg caattcacta atcagttcta
     2521 ctttgtacgg atcacttgca aaaatttcta atgcttcctg ttttgaaaga acacggcgtt
     2581 cgatcggcag attttctttg acgattttca tcatttctgc ttcgattgcc ggcaaatctt
     2641 ctgcagttac ttggttttgt ccattatctg tatcgtagta aaaccctgaa tcgatagctg
     2701 gtccaacgcc aaagtggata tttggataga ggcgacgcat ggcttgggcc atcaaatgag
     2761 ctgctgaatg acggactaag ccaagtgcat cttcatggtc tggtgtgatg atctcaatac
     2821 ttgcatcttc ctcgattgga cgagtcaaat caacgagttt tccgttcact ttaccagcta
     2881 aagctttttt tgctaagctt tttgaaatgc tttcagcaat tgccaatgtt gttgttccag
     2941 actcaaattc tttgactgca ccatctggaa aagtaatttt tatcataata gtttcctcct
     3001 tgaaatgttt tgattgatca gatcatcaag ctgaaaaaca aaaaaatcct agcatctctt
     3061 gaaaagagat actaggacga aaagttcgtg gttccaccta atgttcaaac gcaaatgcgt
     3121 tcctcaagcg ttgtaacggt ccgaccgccc ttgcttcaac aagggtttcg aaagtggtca
     3181 tcctttggtt ttctcctgga aaagtttcag cctagctttt cctctcttat cgaagactct
     3241 caaattcagt tggctttctc atcaatcaat tatgtgttta tttaaccact aataaataga
     3301 aaaatcaagt gggaaactaa acttattttt tccattcccc atttacaccg tatgttcctt
     3361 ttttcatctc attgatgatg acatggatgt gttcttttgg tgcctgcgtg tttttatgaa
     3421 cagcttccgt gatatctttc atcatatttt ctaattgttc cggactgcga ccttctacta
     3481 attcgacatg tacgaatggc ataatgctct ctcctctttc tgatcgttct tttttcaact
     3541 cgattctatc atactatgtt tgtcttgggt actgaatcag gaaaaatggc tggatctttt
     3601 tttgctctca aggcactcaa aatagagacc gtatccacga cttcttgcaa aacagctccg
     3661 aataatgcgg ggattagtcc cgtacttgca attaacatca aaaaaataca aataaagatg
     3721 ccaatcagta cagactgttt cgctattctc atcgtatctc ttgaaatcac tactgctttt
     3781 gctacacgca agagatcatc tttcaagatg accgcatctg cactttcact agcagctgta
     3841 gaaccatgag cccccattgc aataccgata tctgcagcag caagtgctgg tgcgtcattc
     3901 acgccgtctc ctaccatcgc aacaggtctt tgatctttag ggatactttc taaaatggtc
     3961 agtttatctt gaggcaagct acgggcatgt acagaatcta attggacttt ttctgcaatc
     4021 gtttgagcaa tggcagatcc atccccggtg atcatcatca aatttttcac atcaagtttt
     4081 cttagctcct tgattgtctt tgctgcttct gggcgaatct gatccgaaaa taggatcgtt
     4141 cctgcaaaag atcgatcaat tgttacatag attgctgtat tggacgtttc tatttcttca
     4201 gctgaaacaa aagaggcacg tccaatcttt acttcatgat tattgactac tgcttggatt
     4261 ccttgaccgg tcacttcttt cagttgacta acaggataca gttggctgtt tttcacgttg
     4321 ttcactaacg aacgagctaa gatatggcta gattcctgtt ctgcactagc tgcatacaga
     4381 agtagctctt ctttttttat tccattgact ggctgaatcg cctctacttc caatatccct
     4441 ttggtaagcg ttcctgtttt atcaaaagca atcgtttttg tacgtgcgag cttttcaatc
     4501 gttgttccag tttttacaac gatcccgttt ttacttgaac ggctcattcc tgcaactaaa
     4561 gcaatgggcg cagctagaat cagtggacaa ggtgatgcca ctaccagtac ctcagcaaat
     4621 cgtaaagggt cttttgagaa ccaccaagaa ataccaccga tcaaataagc aatcaacgtg
     4681 aaagggactg cataacgatc agctaaacgg acaaatcggg caggcttttc ttttgattct
     4741 tcgactagtt taataatcat ttgatactgg ctgtctgcgg cacgtttttc agcgatcatt
     4801 tggatcgatc cgtctccatt taaagaccct gacataatct gatctccagg tttcttggtt
     4861 tccgggcggg actcacctgt caaagaagat tcatcgaccg aagattctcc tttggtcact
     4921 cgaccatctg cgggtacaag ttcgccaggc ttgactagta actggtcatc aatatttatt
     4981 tcatcgatta ctatatcctg caattttcct tctttgaatc gatgggcaat tcttggggtg
     5041 ttatctaaca aagactgcag ttctttaccc gctcgtttat tcgcgtaatc ctctagacta
     5101 tctccgcccg taagcatgat caatatcatc aaactagccc agtattcttt gacggctaat
     5161 gttgcaagga ttgcggtaat cgctaataca tctacgccat atcttcctga acgtaacgtc
     5221 ttgatcattt ccaaaaacat caagaaggcg agaatccctc cagtaaatgc gacaatcgca
     5281 aatgcaaggg ccggctggtg aaaaacaaat tcacttaata aagctatccc accaataagc
     5341 aaagtaagta aaaatttttt gaaatgtgtc attgtttttt ctcctctttt atgtgactta
     5401 gagaggttag taataaattt ccagctgcct ctttcatctt aagcatagtc cttgactagc
     5461 actttttaaa acaataggcc ttaattgaaa acaactatca ctatcagcgt ttccaaaatt
     5521 catcagttct acaaacaaaa atattcagca aggattactt tttcgattca tcagttaaaa
     5581 agtacctttt ttcgaaaaac tatgctatac ttttaattcg ttatttacgg cggaaggagc
     5641 aaacatgcga tgaatgagaa acaactagaa caaacgcacg tggatgaaac gatcgaacta
     5701 atccagatcg aacaaaatat gctggataca aaacaacaag aattgactca ggaaatgaat
     5761 cattcgacaa aagacacagc taatcacaag attcgagctg gttccaatga agctttttat
     5821 gaatctgctg ttgaatatcg ccaacatgag caggaattgc ttttgaaata ccatactctt
     5881 gaagcacagc aaaaacgcct gcaaacgttg tctgtcatga aagatagtcc ttattttgcc
     5941 cgtatcgact ttaaagaaga ccgtgaaaca gaaacattat atctaggtat cgcttcttta
     6001 agagatctgg cagaagaaac aatcgttatt gactggcgcg caccaattgc caatctttat
     6061 tatgaaggtg agattggtcc cgcattttat gaaactgaaa cagatagaat agatgtcgat
     6121 ctgcagttga aacgacaatt taaaatccaa gaaggacgta ttttatctat ggtagatacg
     6181 tcggaagtaa tcaatgatga ctttttgcta gagatattgg atgaatcatc aagcaaccat
     6241 atgaaaaaca tcgtctctac gatacaaaaa gcacaaaatg caatcattcg ggacacaaca
     6301 agtaaagtaa tgctgattga aggaatcgcc ggaagtggaa agacatccgc tttgcttcaa
     6361 cgtattgctt ttctgttgta tcataatcgc aagtggctcg aggctgaaaa cgttcttcta
     6421 ttttcaccca accatttgtt ttctgattac atctcgactg tattgccttc attaggtgaa
     6481 agcggcgtgc ctacacagac attcaaaaat tatttggtgc agctgctgcc agagttttct
     6541 ttactagatg aacagcaaca agaaagtggt tttttgctag gggaaaaaga tccgatacaa
     6601 gtgatgaaaa gcggattgac cttagtcgat cagattgatc gctatatcca aggcatcaca
     6661 aactacggtc ccttgttccg tgatatgaaa ataaatggcc gtacgatcct atcaaaggaa
     6721 tcgattcgcc aatggtataa agaaacaaat gaacaattac ctttacatca tagactttct
     6781 ctattacaaa cgaagctcct gaaaaaatta ggcggtctgc aaaaagacga aacacgtcag
     6841 caatgggtca aagatctggc ggaagaacag ctccaagaac tatacgcaac agatccaaat
     6901 cttgaatata cagaacaaaa ggagcgttca ttacgtaaaa aactggccaa ccaaatcgta
     6961 cgtaaaagat tccgtcgaat ccatcgaggg atcatgaatt atcaatttgt caatataacg
     7021 aaacaatatc tccactttct acaaactgtg ccactttctc ttctaacacg acataaaatc
     7081 agcgagacca ggtggcagga acatttgaca actgttcgta cacacttgcg tcaaagagaa
     7141 ttgcgtcaag aggatgctgt actattcttt ttactgatgc gccgaacata tccagtgacg
     7201 gttacacaca aagctcgcta tatctttatt gatgaaatgc aggatttcgc accatcacaa
     7261 gtggcattgc tgcaagcctt gttcccaaaa gcaaatttga ctttctgtgg agatctaaat
     7321 caaaatgtct ttggaaatga aacgatcgtt ggatcattgg aagacctttt cgtggaccgt
     7381 gaagtcactc gtttccaatt aacgacaagc tatcgctcca caaaagaaat cactgatttt
     7441 gctaatcatt ttattgtgaa agaacatcaa gtagaaacaa cagcaagaaa aggagacctt
     7501 cctttgctca tccaaggaac aacagatgat atcaaattga attggttagc agataccatt
     7561 cgaacgactc agggaaaagc ccggtattgg cgtacagcaa tcatagctaa aacacaagaa
     7621 gaatgcgaag ctctttatgc tcaactgcca gacgagttga aacaacaagt ccagctgatt
     7681 ttaaatgaga gtgactttat gaagcgttcc attattttac ttccagcctt tttagcaaaa
     7741 ggtctggagt ttgaccgagt tttcttgtgg gatgtagatg cagaacattt ttcttctcag
     7801 caagacaaat tgatccttta caccatgtgt acacgtgcga tgcatgaatt gacccttttt
     7861 acctccaata atgaaagtcc tttgttaaac aatatggata tttcatctta tgagaaaaaa
     7921 gtactgtaag taaaaagaca actgctatca ttaatgttag cagttgtctt tttatttaca
     7981 ataataattg cagaaaaagt aattcaatca tttaaggtca tttattctat atataaatcc
     8041 aaatctgttt caggatattt taatttgaca agcgaaataa tcattttcag ttcttccatg
     8101 ttttctggat attctgggtc aaataccttg ctatgacggc caatcccgcc attgtcgatc
     8161 aatcggtaaa ttcgattcca ttcaatgaat tggtcaattg attggaaatt gctgattgga
     8221 tagccgccac aatccaaaat ctctgataca cgaaatgcta ggtatgcact atcgatcact
     8281 ttattttttt tcgtgtctat cttctcattt ctttcgataa gaattttttc aggacgatgt
     8341 ttcaagctcc actctaatag tagaaagaaa gcctccatcc gatactcttc ttcaaataac
     8401 cgaaaggctg aatcagaacg acgaaaaagc agcggaatca gataattttg tctataaatc
     8461 agcattggtg cgcggtccac catacactct aaataacggt tcagataccc tgtaccgaca
     8521 tagtttccta atcgatagcc ttcaacgccg aatcttgcgt atttttgcgc taattttgaa
     8581 caatcagtta cgttcacagc taactcttct actttgcggt aaccgcttaa gatacgttca
     8641 aaaggttcta aagtgatttt tgcttccata ttcttccccg tccatcttcc acatacttca
     8701 tatcatttca catgatactt atgctctcat cataccacag gttgaattag aaaagaaaat
     8761 aaatcacaca aaaaagcaag acaatctgtt agtgagattg tcttgctttt cgaaaaagac
     8821 tgaatacttt tcgttaaaac tggttgctta gtcttctaat gccacattat ggaacacatg
     8881 ctgtacatct tctagatctt ccagtgcatc aatcattttt tcaaatgttt cttgatcttc
     8941 tggagatagt gttacctcgt tttgagggat catttggatc tcagcgattg aaaaatcagt
     9001 gattcctttt tcttttaatg cttcttgtat actatggaaa tccgctactt caccgtaaac
     9061 gatgatttgt ccttcttctt ccattacatc gcgaacatcg atgtcttttt ccattaggta
     9121 ttcaaggatc tcgtcagcgt cttctcctgc aaaagcaaag attgctgtat tgtcaaacat
     9181 gtaagaaacc gctccggaaa cacccatgtt cccgccattt ttcccaaatg ctgcccgtac
     9241 gtctgctgct gttcgattca cattgttcgt caatgtatca acaatcacca ttgagccgct
     9301 tggtccaaat ccttcatagc gcaattcaga gtatgtttca tctcctgcac cttttgcttt
     9361 ttcgattgct cggtcaataa tatgttttgg tacattataa gttttagcgc gatcaatgac
     9421 gaaacgtagt ttttggtttg catgcggatc aggatcacct gattttgctg ccgcgtagat
     9481 ttcaatccca aattttgcat aaatccggct attattagca tctttagcag ttttctttgc
     9541 tacgatattt gcccatttac gtcccatatt ttcacttcgc tttctgattt gatagtttca
     9601 taacgcaaaa acgtcctgtt ccattatact tgtgaatcac gattctgtga agcttttttt
     9661 ctttcttcct tcattcttct catttttagt caattatcta tactcggatt gggatttgcc
     9721 cgaaattctg ttccggaata ttgcctccca attgatagat ttgatcactt ctaactaaca
     9781 gttccgcttc ttgtgcctct ttttttccga ttcgggaata aatagaaatc tctttttttt
     9841 cttttaagaa tcttcttcca gcatcggtaa atccaagtag tcgaagataa tttttatttt
     9901 gttggacagg aatctcttgc tttttgatat tcagcaagac ataacaagat aatcgttgga
     9961 tccgtgtcca tgtatatctt tttgttttta cctttgacac aaaatcctca aaactatcag
    10021 cagttgcagc tgctgttttc atgcgcgatt ctattccttc ggtcatctga tagatatctg
    10081 tcagttccag caaagtagaa gataacaacc gataacgcaa atacggccaa taagattccc
    10141 aagtctgcag cgaacaagtt gaaagatctt cttctgtctg aggcggcaca agtccagcta
    10201 ttctttttcc ttcttctaac gcatggcgaa tggctgttgc actggctatc ctctgatctg
    10261 tgatgatttt ttcatgatag cctgcacttt tacgtcggat cggatgtaat tccatagggt
    10321 ggacatactt ggcattttcc tttgcataac ttaatcccaa gatatgattt ggcgaagaaa
    10381 aatcaagcgt aatatcggga tacagactgc gaaaaacctc agtcattttt tgcggataac
    10441 tatacgtttg atcagacagt tcttgaaatg cctcttctat cctatcttga ttctcttgga
    10501 caaagcgtcc gaaactgctg taatcaaacg aagtctcttc ttctgttcca aaacacaaac
    10561 tgtcacagtc cagtgtttgc aagattttta ctgcaccttt agcaaaatag tcagctgact
    10621 gaaccgccca ttcgacggga agctccacta ctaaatccac accattttgt aaagcttcat
    10681 tcgcgcgatg ccatttatcg atgatagccg gttcgcctct ttgcaaaaaa ttcccgctca
    10741 ttacagcaat gacgacctct gctcctgtta tttttcgtgc catttccaca tgatagcgat
    10801 gtccattgtg aaaaggattg tactctacga taatcccgca gcttttcatg cgcatcctcc
    10861 ttcatagtta tcgataaaaa agaaatagag acaagtaaaa aaaggcaaaa atcggaaaaa
    10921 atacccgaac tatataaact attctcgcaa acacaatact tctgcgacga aaagctgcac
    10981 tgcaagagca atgcctatat cgttcggata tataatttat gttgaagatt tattcttttc
    11041 ttgttaactc gagcaggcaa aaccgctagt ctttgtatgc tacaaagaac cagcgctttg
    11101 atttctctga tgctgcttcg tctgtaaaat cagaaaatgc ttcgactttc gaaaagccgc
    11161 tgttttccag cattcttaaa taactttcca atggataggt acgctcttga tgtagttcat
    11221 cttcacgaat aaatcgacca gacccttctt gttcttcaac gaaaaaggta agaaaatgtt
    11281 cgatgctgtg tttctcgctt cctggataac tatcccacaa aaaagcaaaa ttatcccctt
    11341 gataatgata gctatattct ggaaaaacct catctacttg ataggtggag tgtacgtcaa
    11401 ataaaaatcg gccattttct tctaacgctt gatagacttc atcaaacact tgctgtacct
    11461 cttgtctatt tttcatatag cacagcgaat cagaaaaaca ggtaattgcc tgatattgtc
    11521 cgatttctga taaatcgagc atattgcctt ccacaaattg gatcgatgcg tcttcttcat
    11581 atgctcgctg gctggcaatc atcaacatct cttctgaaag gtctaaccct gtcactttat
    11641 atccatcttt tgcaaaggca accgctaatg ctccagtacc gcatgccagt tccaaaatat
    11701 tttttgtgtc ttcttgtaaa tgacgtttcg aaaaagctaa ccattgctga taaagtgttt
    11761 cgtccatcac ttcatcgtaa atgaaagcaa atgtttcata agccatatgt taatccaacc
    11821 attcactaag atcgactagc ggagcatctg accatagttt ttccagatta tagaaatttc
    11881 gttctggcgc atggaaaaca tgtacgatca catctccaag atcgatcaat acccattttc
    11941 cgccttcttt tccttcaata cgttttactt catatttgtt ttcttcttct ttatcaatga
    12001 tttcttctgt aattgcgttg atttgacgtt cgctatttgc tgaacagatc atgaaataat
    12061 ctgccaacaa ggaaacattt cgaacgtcta aagcgataat gtcttctgcc cgttttgaat
    12121 ctgctgcttc aatcgcaatt tttaattgtt ctttactatc tatgatgatt tcctcctaat
    12181 atgagcagct tatttttttg ctacccagta attatatgtt tcgattgttt ttggataaat
    12241 ctgttgttct tgcgcaagca aatgggccaa tgtatgtttt gtttcaaatg ctacagcttc
    12301 gtctagatca gcccatgcaa tggctcgtgc atcttgtacc ccaggaaaat ctcgacctgg
    12361 ttcaatatag tctgctacat aaatgatttt gtcaaggaga ctcatcttag cagccccagt
    12421 cgtatgcacc cggattgcat gaagaatctc tgcatcggtg attcctaatt cccgctctac
    12481 aaacgaagcg ccaacaagac catgccagat tgcattatta taattcaata actccggatc
    12541 aaaaccgtct cttacgatta ccattttgaa ctcctcatct ggtcgttctt ttgcataatc
    12601 atgtgtcaat gcagcaatac tagctttttc aggagaagca ccgtattttt tggcaagagc
    12661 cacggctgtt tcttctactc caagcacatg cttgaagcgt tgttcgctca ttctcatctg
    12721 tattttttgc atcaggtttt ctctagaata agcagtatat tcattactat agtccatatt
    12781 catacaaccc ttcattttga atataatcaa ttactttatc tggaacaagg tagcggatag
    12841 aacagccttc tttgatcttt tgacgtattt tcgtggaact gatgtcgatc tcaggtacat
    12901 ctacccaaat tacaggatat ggagtatccg ttgtgtatcc tggtcgacgt atacccacaa
    12961 agttgaccat agaagtcagc tcatcgattt tataccattt tggcaaatat tctaccatat
    13021 ccccaccgat aataaagtaa taatctgtat ccggattgtt ttgcgtcaat tccttcatag
    13081 tatcataggt gtaacttttc ccaccacgag ctaattcaat cgtttcaatc tgcaaaaaag
    13141 gattgtcttc caccgctaat tccaacatgt tgagtcgatg ttttgcatca atcgtctgtt
    13201 tctcatcaac atgaggtggc tgataagaag gcatcaaaaa tacgcggtca agacctaaat
    13261 tccggccagc ttgttcagcc atcactaaat gtgcaaggtg aacgggattg aatgttccgc
    13321 ccaagatccc tacttgcttt ttcttctcta agaattgagg catttcttca ggaaataatt
    13381 gtgaacgaac aaatgtctta gcttgcgtat tcatcatggt cactccttat attgctttta
    13441 cttcttttga tagcctttga tacttttctt ttgtggaagg tttgtataat acaataacac
    13501 gtccaatgat ttggacgatt tcgcaatcta tttctttttc taatacttga gctgcttctt
    13561 ctgcaatctc atctgtattt tgaagtaacg atactttgat cagttctctt ttttctaaag
    13621 cttcttcaat ttgaacgatc attgctgaat tgatcccacc ttttccgatt tgaaaaatcg
    13681 gctgaagatg atgcgctttg ctacgtaaaa aacgcttttg ttttcctctt aaactcataa
    13741 ttttccttct tccttaaatc aatgctttgc gtttcaatac atctacaccc tttggcgccc
    13801 agcccgcaat tactactggc gctgttacag tgatccagcc tagaccggca aaaacgatgt
    13861 ctgttttttc tttcacagaa aactcaaaac gaacaagctc cggaaatgcc tcgacttcat
    13921 caggacgagg aggctgtaat aatccaccta catgtttttg ataaaattcg tctgcttttt
    13981 ccagtttcgt tcgatgaata tttacatcat ttgacacata agcaataaat gaagcacgat
    14041 tgccgctgat aaaatcaaat cttgcaagtc cgcccaagaa aagtgtttgc ccttcattca
    14101 gctgataaac tttaggtttg atttctttta acggtgcaat gatccgcaaa tcttttttac
    14161 ctaagtagtg cgccatttga tggcgatgga taattcccgg tgtatcaatc aaaaaatgcc
    14221 cgtcatctaa aggaatctcg attttatcca aggtcgttcc tggaaactgc gaagttgtga
    14281 ttacatcttg tactcccgct gtatttttga tgatttgatt gataagcgtc gattttccga
    14341 catttgttac tccgacgata tagacatccc gtccatcgcg gtattcttcg attttttcta
    14401 atagttccgt catctcatga gatttcttag cactagttaa taatacatct actggacgaa
    14461 ttcctgcttc gtaagcacgt tccttcatcc attgtgttaa tttagttttt ttcaatgatt
    14521 taggtaatat atccactttg ttcccaacta aaagtactgg attgtcaccg acaaaacggt
    14581 gtagtcctgg gattagtgaa ccattgaagt caaaaatatc caccacattg acgatcaatg
    14641 catctttttg ccctaattca ttcagtaagc gcaaaaagtc gtcatccgtc aatgagacat
    14701 cttgggtctc attataatgg cgtaatctga aacaacgctg acaatagact tccccggaag
    14761 ctaaaccttt ttccaaagcc gtttgcggcg tgtatcctaa ttcaccgggt ttgtttgttt
    14821 gtataacggc accacagccg atacattgta attcttctgt cattcgattc cacctcgcca
    14881 gcccatatca gggtgttttg ctaatagata ggccataatt cttttttccc aaaaacgatt
    14941 aaattgtgtc ttccaactat ctgttgcgat aatgggtttt acaaggatgc tacgtatccc
    15001 tgcacgattg gctccacgta tatcagtcat aatctgatcg cctaccatta ctatctcatc
    15061 atctgctaaa tccaattgtt tctttgctat gttgatccct cttgctaaag gtttcattgc
    15121 tcgtgcaaca tacaaaagat caaatttttc aatcgctcgc gcgacacgtt ttgaattatt
    15181 gtttgaaaca acaacaacag ggattcctgc atttttcatt tctaaaatcc aagtaagcaa
    15241 ctcttctgtt ccatctggat tattccatgc aatcaacgta ttgtctaaat cggtcaatac
    15301 tgctttgatg cctagattct ttaattgggc aggtgtaatc tgatagatgg cttctaccat
    15361 ccatgttggt ttgtaatttg aaaacatgct gtctcctcat ctgcaaaaaa tgaaggagca
    15421 cataaagtac tcctcaaccc cgtattatac tgcatatcag caaaaaaaac tacctctata
    15481 ctcgttcgtc tttcagaaaa agcatttctt tacaaaatga ccaaaaataa agattgatca
    15541 cagaaaaatt tagaaacaaa aaacaactaa tattggcgga acctcttgta tcgtttttcc
    15601 agtaatttat gctgatctaa tcgaacaagt tccgtcaatc tttcaatcaa cgcttttttc
    15661 ataatgtgtg cgactttata tcgtggcaac tgctcgccgt tgaacgtttc aggaataact
    15721 cgatcaacga ttcctaactc gcgtagttct ggcgcagtaa ttttcatcaa ctcagctgct
    15781 tctgcggcgc gactaccgtc tttccacaaa atcgacgcaa atccttctgg agaaagcaca
    15841 gcataaatcg tttcttccag catccagact tcatccgcta aacctagagc aagggcaccg
    15901 ccacttccac cttcaccaat gatgatggcg atgatcggta cctttaaatc agacatttcg
    15961 agcagatttc tggcaatagc ctctccttcg ccgcgctctt ctgctcctac accgcaatat
    16021 gcacctgctg tattgatcaa ggtaatcacc ggtctattga acttttccgc ttgtttcatc
    16081 agtcgtaaag cttttcgata gccttcagga tggggagaac caaaatttct gcggatattt
    16141 tcctgcagat cccgtccttt ttgaataccg atcactgtta ccggtttgcc atctaatcga
    16201 gcgattccac caacaatcgc ttcgtcatct gcaaataaac gatcaccatg gcattcctcg
    16261 aaagaatcga agatctcttc aaaaaaatcc aagcttgtcc atcgttcttt agctcttgct
    16321 aactgaacga catcatgtgc tgtcttatcc acgatctatc catcctttct gtgtatgcaa
    16381 ttcaagaagc gtatggattt tctgtttcaa ttccatgcgg ggaacaatct gatcgacaaa
    16441 accatgagat aataagaatt cagccttttg gaaatcttct ggaagttcct gcttgatcgt
    16501 ctgttcgatg acgcgtcttc cagcaaaacc gatcaagctt tggggttctg ctagaataat
    16561 atcgccttcc attgcaaaac tggcagttac tcctccggtc gtcgggtccg ttaaaacagt
    16621 tagataaaaa agaccttcat tgctatgccg tttgacagca gcagagattt ttgccatctg
    16681 cattagagaa aagattcctt cttgcatccg tgcgccacct gaagctgtga acaagacgac
    16741 tggaagactt tcctttgttg ctcgttcgaa taatctggtg atcttttctc cgacaatcgt
    16801 tcccatactt cccatgataa aattaggatc catcactcca ataatgaagg gaatcccctg
    16861 aatggttgct ttaccagtga ggactgcttc atgaagtcct gttttatctt gcattttttt
    16921 aattttttcc gtatattcgg gaaatgctag cggatctttg gttacaagat ccgtatccca
    16981 ttcttcaaaa cttttttcat caataatcaa agctagacgc tgccatgcac cgattcgaaa
    17041 agcgtatccg caatgagggc atactttttc ttcaccgatg tctttcgtat aaaggatatg
    17101 tttgcaattt ggacactttg cccacatgtt atcaggaaca gatggcgagt tagcaggttt
    17161 gctctctcta ttaggattga tccgaatata tttctttttc ttaaacagag ccatctatgc
    17221 gcctcctatc tccgtttctg gtgtccaatt cggtaaaaat tcttcttgta aaaatgctgt
    17281 gctgtaatca cctgctatga ctgcaggatg actaatcaaa tccatctgaa attctgcgtt
    17341 cgtgatgatt ccttctgtga taaattcact taaggctcgc tgcatcttca ttaatgcttc
    17401 aaaacgcgta tttccatgga cgattatttt tgcaatcatt gaatcataat atggcgggat
    17461 cgtatatccc gggtaaactg cactgtctac tcgcagtccc atcccacctg ctggaagcag
    17521 taagttttgg atcttccctg gagaaggagc aaaatggaaa gcaggatttt ctgcattaat
    17581 ccggcattcg attgcgtgtc cttggaatac tacttcttct tgacacacat tcaaaggctc
    17641 accggctgct atctccacct gtttttttac taaatcgatt cctgtgacca tttctgttac
    17701 tggatgttcc acttggattc gagtattcat ttccatgaaa taaaaatcgc cttcttcatc
    17761 catcagaaac tcgatcgttc cggcattttc ataattgact gctttagctg ctcgaacagc
    17821 agcgtctcct agtgcttctc tttttgtttg tgagatcact acagaaggag attcttccag
    17881 tactttttga ttgttgcgtt gtaaagaaca atctcgctcg cctaaatgaa tgacatgacc
    17941 aaatcgatca cctaagatct gaacttctat atggcgagca ggataaatga ttttttccaa
    18001 atacatatca tcatttccaa atgccgctgc tgcttcttgc tgagctgaag aaaaatgctg
    18061 tgggagctct tcttttgatt gaactttccg aatgcctttc ccgccaccac ctgcagctgc
    18121 tttcagcatc actggataac caattctgtc tgctatttcc aatgcttctt ctactgtaga
    18181 taatacacca gtacttcctg gaatgactgg gacatttgct tcttgcatca attgacgcgc
    18241 attgatctta tttcccattg catcgatcgt tgcactttta ggaccaataa atgtgatatt
    18301 gcactcttca cacatggagg caaatcggct attttcagat aaaaaaccaa atcctggatg
    18361 aattgcttcg gcttttgtca cgattgcagc gctcagtact tcctgaacat tcagatatga
    18421 atcaacggct tttgccgggc caatgcagat tgcttcatct gctaactgtg tatgaagtgc
    18481 ttcggcatct gcttctgaat atatcgctac ggtttggatc cctagttcgc ggcatgcacg
    18541 aatgatacgt acagcgattt caccgcggtt agctatcaat actttagaaa acatatttca
    18601 cctatccaat cataaaggtt aattccgctt cggctacttt tttaccgtct actttagcga
    18661 ttcctttgcc tattccggct gaagctttca ctttcaatat ctcaacttca aggtataagg
    18721 tatctcccgg tgtaactttc ttacggaatt ttgctttgtc caatccgccg aaataagccg
    18781 tctttccctt gaattgttca agagaaagta atgcaactgc tcctgcttga gccattgctt
    18841 caacaatcaa tacccctggc ataacaggtt cttctggaaa atgtccttga aaaaagggct
    18901 cattgatcgt tacatttttc ttagcaacga tccgttctcc ttcaaccatt tcttccactc
    18961 gatcaatcaa aagcattgga taacgatgtg gaatgatttc ttttatttct tgtatgttca
    19021 ttgttttccc tctttcctgc tttgcttatt tttgctgaat cctaaagagt ggctgtccat
    19081 attcgacaac tgactcgttt tctacaagga tttctgtgat cactccgtcg gcctcactag
    19141 ttatctcatt catcagtttc atcgcttcaa taatgcagat aacttctcct ttggcaactt
    19201 gatcacctac ttttttgaaa gctggtttgt cgggcgctgg ttgcagataa acgattccca
    19261 cgattggcga aacaacctcg attccatcaa atactgccga ttctttttct ttcattattt
    19321 ctggagcagt tgttgcttgt tcaggtatga cattgactgg ctccgaaact ggcaacgttt
    19381 cgtttgtttg aaccatattc atgcctcgac ttgttttatt tttattcata tacaattcaa
    19441 attgaccttc acgcaaatcg aattccgtta gcgaggattg atcaaattga ctgactaatt
    19501 ctctgatttc attgatatcc aaaattattc ctcccagcgt ttcatgcaca gaaccgcatt
    19561 atgtccgcca aaccctaatg aattactcat tgcataagta aaacttgcag gtttgctttc
    19621 gtttttgaca actgtcaagt caatctcagg atcttgtttt tctacattga tcgtaggtgg
    19681 gataaattga tgttctaatg ccagcaaagt tgccaaggct tcgatcccgc cggctgctcc
    19741 caataaatgt ccagtcatcg atttcgtgct gctgacgtaa gtatctctga aattttcacc
    19801 taatgcgatt tggattgctt ttgcttctgc ctggtcattg gctggtgtac ttgttccgtg
    19861 agcattgata tatccgactt gttccggctg tacaccagct tcttccatcg caagcttcat
    19921 tgctttagat gcaccagaac catctggagt aggagctgtc atatggtaag cgtcacagtt
    19981 tgcaccgtat ccaacgattt ctccatagat tttggcaccg cgagcttgtg catgttctaa
    20041 agattcaaga actagaacgc ctgctccttc acccatcaca aaaccattcc gattttcgtc
    20101 aaacggaatc gaaccacgat taggatcagt tgattctgag agtgctgtta aagctgcaaa
    20161 tccagcaatc cccatctcac aaatcgttgc ttctgttcct ccagcaagaa tgacatcaga
    20221 atagccatgt ttgatgttac ggaatgcttc cccaattgaa ttcgttccgc tagcacaagc
    20281 tgttacgata gctgtacaaa taccttttgc acctacacgc aaagcaatat ttccagctgc
    20341 catgttggca atcgccattg gtacgaacat tggagaaact cgttttgcac ctttttcatg
    20401 tagacgaata acttggtttt cgattgttgg aagaccaccg atcccagatc cgatgatgac
    20461 gccaaaccgg tcttgattca ttttttcttt atctaaaccg ctcatctcca ttgcttctaa
    20521 agcagcgtag attccataga tcgaaaacat atccattcgt ttgctatctt ttttaataaa
    20581 atatttatcg taagggaaat ctttgacttc acccgctaag gtaatccctg tttctgaagc
    20641 atcaaattta gtgatcggtg cgatgccatt cgtcccctct tttaagctat tcaaaaagct
    20701 gtctgcgtca tttccaatag gtgaagtgac tccatagcca gtaattacca ctcgattcat
    20761 ttgtttgctc tccttttctt atccgtgcat gaccaatccg ccatcgacat tgatgacttg
    20821 tccggtgata tatggatttt gtgctaagaa aactgctgtt tgtgcaatat cttctacttg
    20881 tccgaaacgc tgcatcggaa tttgcttttc cgcttgttcc ttgactttgt ctgctaaaac
    20941 ttccgtcata tcagttgtga tgaatcctgg cgcaatggca ttacatgtaa tgcctctagt
    21001 tgcagcttct cgtgccacag attttgttaa tcctactacg ccagctttac tagctgcata
    21061 atttgcttga ccaatatttc caatcaaccc agatacacta gagagattaa tgatggcccc
    21121 ttctcgctgc ttcatcattt ttttcaatac gtgctgcgtc atattgaagg ttccaatcaa
    21181 attgatatcc agacattttt tgaaatcttc tgcatccatt cgcatcacaa gtttgtcatt
    21241 tgtgattccg gcattgttta ctaacacatg gatcgatcca agcttttctt ctgcttcttt
    21301 gatcatttgt cccgctttct cataatcaga gatgtcaccg gaaactccta cgcattttac
    21361 accgaatgct tcgatttctt ctatcttttc ttttggaatc tctccgcgac cattcaaaat
    21421 gatatttgct ccagcttttg caaaagctaa ggccatcgct ttgccgattc ctcttgtact
    21481 acctgtgata aatacatttt ttccttttac atccataacg ttcctcctta tagtattgct
    21541 attgtttcat caaatgtctg cttattctca acacgcagaa ctggtattgt tttatcgatt
    21601 tttttcataa atccgcttaa tacttttccg gggccgactt cgatgacttg ttcgatgccg
    21661 attgttttta acttatggat actctcatag aaacgaacag gttgcatgac ttgctgttca
    21721 agtagtcccg cgattgtttc tttcttcatt atttccgttg ttgtgttgct tatgatagga
    21781 aaagaaggtt cagagaattg aatctgactt aggtccttcg ccagtttttt tgctgccggt
    21841 tctaaaatag ctgtatgaaa aggcccgctg acatttagag ggatcattct tttgaaccct
    21901 ttttctttta atagtaaaac agcttcgtct actgcttttt cttcgccacc aatcacgatc
    21961 tgctgaggtg tattataatt tgcaggagat acgatgccgt acttgctagc ttcatgacag
    22021 ctttcctcga tcgtttcaat cggcgcattc atcactgcaa ccattttccc gcttccagct
    22081 ggggcagctt ctgtcatata agcgcctctt tttgctacta acgcaactgc ttcagtgaag
    22141 gatagcgcat tactagctac taatgccgaa tattctccta aactcagtcc taaagctgca
    22201 tctgggatga ttccatgctc cttcaataag cgataatacg cgatgctgac tgttagaata
    22261 gctggctgtg tatattgcgt ctgatccaaa cgttcatttt ctgtaaagca aagttcagcc
    22321 atatcgtagc cgagaatctc acttgcttca tcaaatgtct gctttacaac ggcttcttca
    22381 tataaatctt ttcccatacc ttgatattgt gcaccttggc cactaaataa gaatgctgtt
    22441 ttcatgtttt tctccaatat atccagaata tatgtttgat tcttcctatt caaaaaggcc
    22501 gagacattcc tatttcagga aatgtcccag cccccaacct aattttaatg attatgcagt
    22561 ttgtttttct acgtaagtca ctaaatcttg aaccgttttg attccgtctt ctgtttcgat
    22621 tttcacgtca aattcatctt caatttcatt gatgatttgg aataaatcca agctgtcagc
    22681 ttctaagtct tcttggatgt cagtagtcaa tttgatttct tcagtttctt ttcctaattc
    22741 ttctgcgatg atttcttgaa ttttt
//
LOCUS       DAYYCY010000027        22051 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000027 DAYYCY010000000
VERSION     DAYYCY010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22051
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.027"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            60..788
                     /gene="purC"
                     /locus_tag="ACHAZB_000867"
     CDS             60..788
                     /gene="purC"
                     /locus_tag="ACHAZB_000867"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFM8613558.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            788..1042
                     /gene="purS"
                     /locus_tag="ACHAZB_000868"
     CDS             788..1042
                     /gene="purS"
                     /locus_tag="ACHAZB_000868"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HFM8613559.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1044..1715
                     /gene="purQ"
                     /locus_tag="ACHAZB_000869"
     CDS             1044..1715
                     /gene="purQ"
                     /locus_tag="ACHAZB_000869"
                     /EC_number="3.5.1.2"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HFM8613560.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            1716..3938
                     /gene="purL"
                     /locus_tag="ACHAZB_000870"
     CDS             1716..3938
                     /gene="purL"
                     /locus_tag="ACHAZB_000870"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HFM8613561.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            3923..5362
                     /gene="purF"
                     /locus_tag="ACHAZB_000871"
     CDS             3923..5362
                     /gene="purF"
                     /locus_tag="ACHAZB_000871"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFM8613562.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            5385..6437
                     /gene="purM"
                     /locus_tag="ACHAZB_000872"
     CDS             5385..6437
                     /gene="purM"
                     /locus_tag="ACHAZB_000872"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFM8613563.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            6434..7012
                     /gene="purN"
                     /locus_tag="ACHAZB_000873"
     CDS             6434..7012
                     /gene="purN"
                     /locus_tag="ACHAZB_000873"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFM8613564.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7009..8544
                     /gene="purH"
                     /locus_tag="ACHAZB_000874"
     CDS             7009..8544
                     /gene="purH"
                     /locus_tag="ACHAZB_000874"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFM8613565.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            8560..9834
                     /gene="purD"
                     /locus_tag="ACHAZB_000875"
     CDS             8560..9834
                     /gene="purD"
                     /locus_tag="ACHAZB_000875"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFM8613566.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            9956..10870
                     /locus_tag="ACHAZB_000876"
     CDS             9956..10870
                     /locus_tag="ACHAZB_000876"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFM8613567.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(11003..12568)
                     /locus_tag="ACHAZB_000877"
     CDS             complement(11003..12568)
                     /locus_tag="ACHAZB_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8613568.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(12561..14012)
                     /locus_tag="ACHAZB_000878"
     CDS             complement(12561..14012)
                     /locus_tag="ACHAZB_000878"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM8613569.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITA
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(14026..15042)
                     /locus_tag="ACHAZB_000879"
     CDS             complement(14026..15042)
                     /locus_tag="ACHAZB_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8613570.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15262..16350
                     /gene="chvE"
                     /locus_tag="ACHAZB_000880"
     CDS             15262..16350
                     /gene="chvE"
                     /locus_tag="ACHAZB_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     substrate-binding protein"
                     /protein_id="HFM8613571.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            16404..17930
                     /gene="mmsA"
                     /locus_tag="ACHAZB_000881"
     CDS             16404..17930
                     /gene="mmsA"
                     /locus_tag="ACHAZB_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM8613572.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            17932..19137
                     /gene="mmsB"
                     /locus_tag="ACHAZB_000882"
     CDS             17932..19137
                     /gene="mmsB"
                     /locus_tag="ACHAZB_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     permease"
                     /protein_id="HFM8613573.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            19394..20371
                     /gene="guaC"
                     /locus_tag="ACHAZB_000883"
     CDS             19394..20371
                     /gene="guaC"
                     /locus_tag="ACHAZB_000883"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFM8613574.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            20635..>22051
                     /locus_tag="ACHAZB_000884"
     CDS             20635..>22051
                     /locus_tag="ACHAZB_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFM8613575.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWL"
BASE COUNT         7103 a         3724 c         5035 g         6189 t
ORIGIN      
        1 aaaaaacgaa caaaaaacga tgtatttgct tttaatgtaa ataaaaagga gagacgtaca
       61 tggagaagaa agaacttttg tatgaaggaa aagccaaaaa aatatatctt acttctgatg
      121 agaacttgat ttggatagaa tatttagatc aagcaactgc gttgaatggc gcgaggaaag
      181 accagataag tggaaaagga gaattgaata atcaaatcac aagtgcaatt ttccgttatc
      241 tgactcaaag aggaataaaa aatcatttta ttgaacaatt gtctaaaacc gaacaattag
      301 caaaaaaagt acaaattatc cctctggaag tggttatccg aaatattgca gcaggaagtt
      361 tctcaaaacg tctaggtgtt cccgaaggta aagaattcat caaaccaatc attgaattct
      421 actataagga tgaccagttg gatgatccat ttatcaatga agagcatatc tatttcttac
      481 aacttgcaaa accggaagaa atcagtgagc tgaaagccca agcgttggct attaatgaaa
      541 ttttgaccga attatttgct aaggtcggtt tggttttagt agatttcaag ctagaatttg
      601 gccggtcgaa tggacaaatt ttgcttgcgg atgaagtaac accggatact tgccgattat
      661 gggatgaacg tacgaatgaa catatggaca aagacgttta ccgcagagat ctaggtgatt
      721 tgatcccagt ctatcaagaa gtatataacc gtctatcgga actaactgaa gaaaaggagc
      781 gagtatgatg tatttcgtaa aagtatatgt aacctataaa gattcagtgt tagatcctca
      841 aggggaggca gtaaaaaaag cagtccatcg tatgggatat gaaacaatcg aagatatccg
      901 aataggaaaa tactttgaga tacaacttgc tgatgcagaa gaaccaatcg atacagtgat
      961 tgaaacgatt tgtgacaagc tgcttgcaaa tgtaaatatg gaaacatacc gatatgaaat
     1021 caaaaaaatg gaggaagtat aacatgaaat ttgcggtcat cgtttttcca ggttcaaatt
     1081 gtgacgcaga tatgttatgg gcagttcgcg agatcatggg cgctgatgcc gaatttgtac
     1141 ggcatgatga gtcctcttta gaaggatttg atggggtttt actgccggga ggattctcct
     1201 atggtgacta tttgagatgc ggtgcgattg ctcggttttc tacaatcatg gaagaagtga
     1261 ttcgttttgc tgaagaaggg aaaccagtat tcggtacatg caatggattc cagatattga
     1321 cagaagcagg acttttgcca ggtgtactga gacgaaatga gtctttgcat tttatttgca
     1381 aaactgtacc attgaaagtc aaccccaaga cggcctttac ttcagagtat gaagccgacg
     1441 aaataatcta tcttccagtt gctcatgggg aaggaaatta tttctgtgac gaagaaacac
     1501 ttcaagaact aaaaaaaaat aatcagattg cctttactta taatgacaac atcaacggca
     1561 gcgtggaaaa cattgcaggg attacgaaca aagcagggaa tgtgttagga atgatgcctc
     1621 atccagaacg tgcagtagaa gacttgctag gatcagaaga tgggaaacgg ttctttgctt
     1681 ccattttaaa aaacttcgga aaggtgaccg tatagatgaa aacattgaaa gaaccgacag
     1741 ctgaagagat caagaaaaaa agaatctatg ccgaatgggg attgactgat gaagaatatc
     1801 gaatgatcga agaagatatc ttaggaagaa tgcctaatta tacggaaact ggactgtttt
     1861 ctgttatgtg gagcgaacat tgttcctata aaaattctaa accggttcta cgcaaatttc
     1921 ctactagtgg acctcatgtg ttgcaaggcc ctggagaagg cgcaggcatc gtcgacatcg
     1981 gtgatggact agcagttgtc ttcaaagcag aaagtcacaa tcatccttca gccgtggagc
     2041 cttatgaagg agctgcgaca ggagttggcg gaatcatcag agatattttc agcatggggg
     2101 ctcgtccaat tgcgttgcta gattcattga ggttcggcga attagataat ccaagaacga
     2161 aatatttact agaagaagta gttgcaggta tcggcggata cggcaattgt atcggcatac
     2221 cgactgtagg cggcgaaatt gcatttgatc cttgttatga aggaaaccca ttagtcaatg
     2281 caatgtgcgt aggtttgatt gaacacaaac atatccagaa agggcaagcc gctggcgtcg
     2341 gtaattcgat catgtatgta ggtgcaaaaa caggtcgcga cggtatccac ggagcaactt
     2401 ttgcctcaga agaatttaat caagaagaag aacagcaacg ttctgctgta caagtaggcg
     2461 atccttttat ggaaaagctg ctgcttgaag cgtgtttgga attaatcttg catcattcgg
     2521 attctctaat aggtatacag gatatgggag cagcaggact tgtttcatct agtgcggaaa
     2581 tggcttcaaa agctggctca ggacttatat tgacattaga tgaagtccca caaagagaaa
     2641 ctgggatgac accttatgaa atgatgttat cggaatcaca agaacggatg ttgatttgtg
     2701 ttaaatcagg agaggaagaa aaaatccaag aactatttca gaagtatgat ctagatgcag
     2761 tcactatcgg taaagtaaca gacgacgggc aatatcggct atatcatcaa ggagaagaag
     2821 tagcaaatct gccagtcgat gcattagctg aagatgcacc agtttatcat aaagaaatga
     2881 aagaaccagc tcgaatcgta gaatttcagc agttagctcc ttatcagcca gttatcgaag
     2941 aacctggaca agtattgctt gatctgctgc agcaaccgac gattgcttca aaacgttcaa
     3001 tctacgaaac ttatgattca caagtacaaa ccaatacagt tgtccgtcca ggaagcgatg
     3061 ctgctgtgat gagggttaga gggacgaata aagcgttagc tatgaccaca gactgcaacg
     3121 cgcgctatct gtatttggat ccaaaaatcg gaggacaaat agcagtggca gaagctgcta
     3181 gaaatattat tgctagcggt ggtaagccct tggcgatcac cgactgcctg aactatggtt
     3241 ctcctgataa accagaagtt ttttgggaat tatcgacttc agctgacgga attgctgctg
     3301 cgtgtgaaac gttaggaact cctgttattt caggaaatgt ctctttatat aatgaaacgg
     3361 atggtcaagc aatctatcca acaccaatga ttggtatggt cggattgatt gaggatcata
     3421 aacatatcac tactcaggaa ttcaaaaaat caggggatct aatctatata ttaggtaaga
     3481 cgtttgcaga ttttgatggc agtgaattac aaaagatgca gcttggaaga atcgaaggtg
     3541 tgatccgaaa ctttgatcta tccattgaaa agagaaacca agagttagta ttgacagcca
     3601 tccaaaacgg attgattgaa agtgctcatg actgttcaga aggtggcttg gcaattgcac
     3661 tggcagaatc agctttcaaa catcagctag ggatatctgt acagtttgaa ttgtcctctg
     3721 ctcaattatt tgcagaaact caatcaagat ttgtgttgac agtcgcaccg gaaaataaga
     3781 cacgattcga agaaatgatg ggagatgctg ctgttttagc aggtaaggtg acggatgaag
     3841 cgataatcga aatttcagca actgacggtc agatcaaaat agaaactgca gtagcaagaa
     3901 agtgctggga ggatgcaatc gtatgtctta tgaagtgaag agtttaaatg aagaatgcgg
     3961 gatcttcggt gtttggggtc atccagacgc cgctagtgtg gcatacttcg ggctccatag
     4021 tcttcagcat cgaggacaag aaggtgcagg tatcgtgtcc aatgatcgag gaaagcttaa
     4081 ggggcatcga gatttggggc tgctttctga ggtattcaaa gatcaaagaa aattgcagca
     4141 gttaacagga gaagctgcaa tcggacatgt acgttatgca acagcaggca atggaagtgt
     4201 ggacaatatt cagccttttc tattcaaatt tcatgatcag tcgatcggct tagcgcataa
     4261 tggtaacttg atcaatgctc gttcccttag aaaagagtta gaggaaaatg gggcgatctt
     4321 ccattctaac tccgatacgg agatattgat gcatttgatc cgacgaagtg aagaaacaga
     4381 ttttttaaat aaattaaaaa gtgctttgaa tgaagtcaaa ggtggctttg cctatttatt
     4441 aatgacagaa acggcaatga ttgccgcttt ggacccaaat ggttttcgtc cattagcaat
     4501 cggacaaatg aaaaacggag catacgtgat tgcctcggaa acttgtgcat tagaagttat
     4561 tggggcacgt tttataagag atgtccagcc tggtgagata gtgatcatca atgacgaagg
     4621 aatccagatt gattgcttta cgaaggacac gcagctttct atttgttcga tggaatatat
     4681 ctattttgcc agaccagatt caaacatcgc agggatcaat gtgcatactg cacggaaaaa
     4741 tatgggacgg aatttagcga aagaatcgcc agtaaaagca gatatggtcg ttggcgtacc
     4801 taattcttct ttgtcagccg caagcggtta tgcggaagct agtggaatcc cttatgaaat
     4861 cggattagta aaaaatcaat acattgcccg aacatttatc cagccaacgc ctgagctaag
     4921 agagcaaggt gttcgaatga aattatctgc tgtacgagga gtagtggaag gtaaaaaggt
     4981 gattatggtc gatgattcaa ttgttcgggg tacgacaagc agacggatcg tccatctttt
     5041 gaaagaagca ggggcaaaag aagtccatgt gaggattgct tctcctcctt tgaaatatcc
     5101 atgtttttac gggattgata ttcaaacgcg taaagaactc attgcggcaa atcacacgat
     5161 tgatgaaatc aaagaatgta tcggtgcaga ttctttaggt tttttaagtg aagagggtct
     5221 gatcgaatcg atcggtctgg aaaaagatgc tccgtattca ggattgtgta tggcttattt
     5281 caacggagat tatccgacgc cgttatatga ctatgaagaa aaatatcagg aatcactaaa
     5341 agaaaaagta tcgttctttt aaaagcagac caagaaggga gtatatgaat tcaatggcaa
     5401 atgcatacgc aaaagctggc gtagatgtcg aagctggata tgaagtagta gaacggatca
     5461 agaagcatgt caaaaaaacg gaacgtttag gtatgatggg cgctatagga ggattcggcg
     5521 gttgttttga tttatcggtc tttcaattaa aagagccagt ccttgtctcg gggaccgatg
     5581 gcgtggggac gaaattagca gtggcaatcg aagcagacaa acacgataca atcgggatcg
     5641 actgcgtagc gatgtgtgtc aatgatatct tagcacaagg cgcagagccg ttatatttct
     5701 tagactactt agcaacgggg aaaaatgatc cgttacgttt ggaaaatgta gtaaaaggtg
     5761 tagcagatgg atgtgttcaa ggaaatatgg cgctgattgg cggagaaaca gctgaaatgc
     5821 ctggtatgta ctcaggtaat gattatgatc tagccggatt tgctgtaggg atagctgaaa
     5881 aatctcaatt attgacagga gaaacgatca acccaggaga tgtgttgctt ggaatagcat
     5941 ctagcgggat tcattcaaat gggtactctc ttgttcgcaa aattttcttt gagcagcagc
     6001 agttttcttt atcatcaaaa ctgccagaat tgaaaaaaac acttggcgaa gaattgcttg
     6061 agccaacgaa aatctatgtt tcttccttgc ttccgctgat caaagaaaag aaaataaaag
     6121 gagctgcaca tatcactggt ggagggtttg tagaaaatat cccgcgaatg ttaccagaat
     6181 ctatggctgc acaaatcaaa cttggctcat ggccgattct tccaatattc ggctgtttac
     6241 aaaaatatgg tgaaattcct gagatggaga tgtatgaaat cttcaatatg ggcatcggta
     6301 tggtcctggc agtcgaagaa tcgaaagtaa aagaaatagt acacgaaatt gagcaagctg
     6361 gcgaaaaagt ttatgaaatc gggaaagtga tcgccaaaga aaaacatcgt gtggtgttta
     6421 taggagaaga acgatgagaa tcgctgtttt tgcttctgga aacggaagca actttcaagc
     6481 gctagccgat tatctatcga aaaaaggatt ggaatcttcg attgactggt tgttttgtga
     6541 tcagcctgaa gcatatgttt tgaaacgtgc aactgcgttg agtgtaccgg cagattgttt
     6601 ttcacctaaa gaatttgatt caaaaaaaga gtatgaagaa gcaattttgc ataaattgaa
     6661 agaaaaaaag atcgatttga tcgtattagc tggatatatg cgaatcattg gaccggtatt
     6721 gctgaaaaat tatgataaaa gaatcatcaa tatccatccg tctcttttac ctgccttccc
     6781 tggtttgcat ggcatacgtg atgcctttga agctggagta aaagaaacgg gtgtcacaat
     6841 tcattatatc gaccaaggtg tagatactgg accaatcatc cggcaggaaa aagtgagaat
     6901 tgaacaggag gacacgttcg attctttaga agaaaaaatc catagagtag agcatcgaat
     6961 ctatccagaa gtgattagtg aaattattga aaatggaggc tatctgaaat gacaagagca
     7021 ttgatcagtg tatcagataa aactgggatt gtagcattcg cccaaggttt gattgaacaa
     7081 ggagtagaaa tcatttcaac aggaggaaca aagcatgcgt tggataaagc tgggatcaaa
     7141 acgctttcga tcgaagaagt gacagggttt cctgaaatga tggatggccg tgtcaaaaca
     7201 cttcatccta aaatccatgg tggtctttta ggaagacgcg atctccctag ccatatggag
     7261 gcaatggaaa cggaaaatat ccaaccaatc gactttgttt gtgtcaattt atatcctttc
     7321 aaagaaacga ttatgaaacc agatgtcgaa atagcagaag ctattgaaaa cattgatatc
     7381 ggtggtccta gtatgctccg tagtgcagca aaaaaccatg aatttgtcac ctctgttgtt
     7441 gatccatctg actatgaaga agtactttca gaattacgtc agtatgggca tacttcactt
     7501 tctttcagac aaaaactagc ggcaaaagtc tttcgtcata ctgcggcata tgatgcactg
     7561 atcgctcaat acttaacgga ttgggtggaa gaaaaagaac cggaaaaatt gacacttacg
     7621 tatgaacgaa aacaaacgct tcgttacggt gaaaatagcc atcaagccgc tgccttttat
     7681 cagtccgctc ttctggaaaa atattcgatc gcagcagcaa aacaactcca tgggaaagaa
     7741 ctttcttata ataatatccg agatgcagat gctgcgctac ggatcatgaa ggaatatgac
     7801 gagccaacgg tagtcgcctt gaaacacatg aatccttgtg gtattgggac agcatctacg
     7861 attttagaag cgtgggaagc agcgtatgaa gcagatcctg tttcaatctt tggtgggatc
     7921 atcgttttga atcgagaagt agatctgcca acagcagaac agatgcataa gctgtttcta
     7981 gaaatcatca ttgccccttc ttatacacaa gaagctttgg aattactgca aaaaaagaaa
     8041 aacatccgat tactgacagt agactttgcg caggaagaaa atcaaaatcg agaagaaacg
     8101 gtctcagtct taggtggact tcttgtacaa gatcaagatc tggcaataga aaatgaagaa
     8161 gagtggaaag tcgtgacaaa acgtaagccg actccagcag aagcaaaagc attagcgttt
     8221 gcttggagag ctgtcaaaca tgtgaaatct aatgcgatcg tactggcaaa cgaacgtcag
     8281 acagtaggaa tcggagccgg gcagatgaat cgagtcggtt cagtaaaaat tgcgattgaa
     8341 caagcgcaag agaaaatgga aggtgcagtt ttagctagcg atgcttattt tccaatggat
     8401 gattcggttg aatatgcagc caaacatggt ataaaagcaa tcattcagcc aggaggaagc
     8461 atcaaagacc aagattctat tgatatggcg aataaatacg atctagcaat ggtctttact
     8521 gatgttcggc attttagaca ttagagagga gtcggataaa tgaacatttt ggtgatcggt
     8581 gcaggtggtc gtgaacatgc aataagtaaa aaacttttag ccagtccgaa ggtggatacg
     8641 gtgtattgtg cgccaggaaa tccggggatg acaaaagatg gtatcaattg tgtcgctatc
     8701 agtgagcgag atcattcagc gttaatccat tttgttaaga aagaggctat tgattggagt
     8761 atcgttggtc ctgaagttcc tttgttaaat gggatcgtcg atgattttat ggcggaagga
     8821 ttgaaaatct tcggtccgac aaaagcagca gctctgatcg aaggttcgaa aagttttgct
     8881 aaacaattaa tggatactta tcaaatcccg acagctaaat ccagaagttt tgaatgttac
     8941 gaaaaagcag cagcgtatat caaagaaaat ggtgcgccaa tcgtagtgaa agcggacggc
     9001 ttagcagctg ggaaaggtgt agtcgtggct accacagtga aggaagcgct ggatgcggca
     9061 gaacaaatgt tgaaccagca tcgatttggg gatagtagca aaaaaatcgt cgtagaagag
     9121 tttttggcag gagaagaatt ctcccttttg gcgtttgtcc gtaataccaa ggtgtatccg
     9181 atggtcattt cgcaagatca taagcgggca tttgataacg ataaagggcc aaatacaggc
     9241 ggaatgggtg cttattcacc agtaccgcaa atttcaaaaa agatgattga aaaagctgtg
     9301 gaagaagttt taaaaccagc ggctttggga atgaaagaac taggacgctc tttcacgggt
     9361 gttttatatg cgggtttgat tgctacgaag gaaggaccaa aagtgatcga attcaatgcg
     9421 cgtttcggtg atcctgaaac gcaagtcgtt ctttctcggt tgaagagtga ctttgcgcaa
     9481 gtaatcgatg atttattaga gaatcgtaca gtagagttaa agtggcagca agaaggatac
     9541 aatgtaggcg ttgtggttgc agctgccggt taccccgaag aatatgagac aggaatggtt
     9601 ctaccagatt ttctggaaga agagctttct gtttactatg ctggagtttc ctcaaataac
     9661 ggcaaactag ttggagcagg aggacgtctt tatttagtag aagcatacgg tgagacgatc
     9721 aaagaagcac agaaaaaaat ttacaccgcg ttaatggata aaacgacaga tggaacattt
     9781 tatcgtacag atattggaga aaaagctgcc aatagcgaac tgatcgtgaa gtaaatcagc
     9841 tgcttccatt tataaaaaat acaacaaaca taatcggaag cgccgatttt agtgaagcct
     9901 tttcataaag agtgtggtac acttttgttg tgtatataaa taaggaggag ttcagatgaa
     9961 aaaaattcaa ttaggtactt ctgatcagaa agcagctgca gtgatcttag gctgtatgcg
    10021 tatcaacaat gcggaagatc cagtaaaagt gattgagaca gcttatgaaa acggcattga
    10081 tttttttgac catgcagata tttatggaaa cggtgaatgt gaagaaatct ttgcagatgc
    10141 tctagcaaaa acatcaatca aacgagaaga tctttttatt caaacaaaat gtggaatcgt
    10201 tccaggtgtg atgtttgatt tttctaaaaa acatatcatt gaatctgtag aaaaaagttt
    10261 gacccgtctg aaaatggatt atgtggatgc tttacttctt catcgtccag atacattggt
    10321 agaaccagag gaagtagctg aagcgtttga tgaattggaa cggtcaggaa aagttcgtta
    10381 ttttggcgtc agcaatcaaa aaccaatgca aatcgaattg ctgaaaaaat atgttcgtca
    10441 accattagta gctaatcagc tgcaatttgg cttgaaacat actggaatga tcgatcaagg
    10501 gattcatgtc aatatgacag atgaagcaag tatcgatcat gacggtagtg tacttgatta
    10561 ttcccgattg catgatatga cgatccaagc atggtcacca taccagtatg gattttttga
    10621 aggtgtattt atcgggaacg aaaaattccc tgaattaaac caaacattag aagaactagc
    10681 agctaaatat caaacgacac caactggctt ggcttctgct tggattttac gccatccagc
    10741 caatatgcaa gtgatcgccg gtacgatgaa cgtagggcga atcaaggaaa tcgctcaagc
    10801 ttcagaaatt aatatgtctc gggaagattg gtacgaattg taccgtgctg ctggaaacat
    10861 tttaccttaa agattgtcat aagcaggtca aaagggctat aaattaaaaa gacagaataa
    10921 cttgaaagct gttttcagct tcgagatatt ctgtcttttt ttattgcaaa tggaaagagt
    10981 atcattgtac ggatttcgtt tattagttat ttttgttttt aaggaaaagc tgtgtccgat
    11041 attcttttgg agaaatccct tgttttttct taaataaata actgaaatag ttagggtcat
    11101 tataaccaat ttcaaaagca atatctgaaa tacgatagtc tgattgctgc aataattttt
    11161 ttgctaattc gattctttgt tctgtcagat aatcgatgaa tgtttggtta aacgattgag
    11221 aaaaaatcgt actgaaatga gagggactta atgcaacttg ttgagcgacc caagttaagg
    11281 acatatctgg atcggtaaaa ttttctttga taaaatccaa tgcatggttc aaaattggct
    11341 gatatttgtt ccttgaaggc tgtgagggca gagcagttaa gaaagaaatg aacatataaa
    11401 ctgtcgaacg gtaaagcgag tcgtcggcgc tgattgctaa gcgttgactg gtggatgtgc
    11461 aatgaaccat ttcttgcggg attttctttt ctttcaggca gacatgctgg agtagcttag
    11521 tcaagacaaa taaacgatat aatcgtgttc gagttggtgt gccttgtggt ttcgtcaagt
    11581 gttcgatgta cttagatact tcgtttttcg ataatgaaga caaatcaagt gagaggtcaa
    11641 agtaaaagac agaatcaact ggaagtttcg cttcttgaat caaatcctca tagctgataa
    11701 tttgttcttt aagggaactg gcggtttcaa gtatcatctg cgtgatctca aaagatgcag
    11761 gtaattcgct gattctttct gcgacaggac caatggcgac agaaaaatta tttttttgtc
    11821 ccttttctaa ttcatggacc aacgtttggg ctacctgata actggtctcg atcacatttt
    11881 cttcaagaga atcaactatt aaaaatttga tgaatcgaga ggaaacagca gagaagatca
    11941 cttgttgatt ctccctaaat aaaaacttca aataatcact gagatgagcg taatcttcaa
    12001 agtctgtact gacttggttt gctgctaata atacggtcat ttttttccct gctaaagagc
    12061 gattgaaaga ttcagcaagt ttcaatgttt cactgatcgt aagtgagcct ttgaacaaac
    12121 cgttcaagaa atgatttttt ttcaattcga aaacaagatc agaagaagtg tgcgtttctt
    12181 tttgctgcaa atctaaagca gttactgctt tttctattgc ttgtttcaat tcgctacttt
    12241 taatcggttt aagcagataa tcatctgcct gtacctggat tgctcctttc atataatcga
    12301 aatcgtcgaa tccactaata aatatgagac gagtccaagg aagtagtttc cttgcttctt
    12361 ttgaaaaagt caacccgtcc atgaaaggca tttggatatc agtcagaatg atgtcgggct
    12421 gtaagtcgat aattgaagac agtgccatct caccatctcc agcttcgccg acataagtga
    12481 ttggataata agaaccaagg acagttattt gttcccgcaa gttattgcga atgaggtatt
    12541 cgtcttcgat gataaaaact ttatacatgt ttgtttcctc caattgtttt gggagataga
    12601 gggatcatca gtgtgacagt tgttccttct cggtatttgc tgtcaatcgt gataggtttt
    12661 tgatcatttc cgtaataaag aaggagacgc ttgtttacgt tgtaaagtcc gtagccgata
    12721 ctgaaatctg aatcgatccc tttggctaac tctttacgta tttctgccag tttttctgca
    12781 gggatgccaa tcccgttgtc tataactttg aattggatca tctcatcaat tttgcatgcg
    12841 gtaatacgaa taaatccttt gtgacggacg tacttcgttc catggtatac agcattttca
    12901 atcaatggct gcaagatcat ttttaaaata gaaacaggaa aaagtgtttc tggtatatcg
    12961 atcttatagt caagcatatg gccgtatctt atttttaaga tgactagata atcattgata
    13021 tggctaattt ctttttcaag aggaatccag tcttgtcctt tgcttaaaga cgtacgaaaa
    13081 aagccggaaa gtgcatgggt catagtcaga gcttcttcaa agcagtcttg ttcgatcaaa
    13141 gaaataatgg catccaaact gttataaata aaatggggag taatctgagc ttgcaataca
    13201 cgaacttcac tttgtgctaa atggtattgt ttcaaagcgt tctcccgtaa agactggttt
    13261 agatcttccg ccattttatt catgctagcg gtaagtgttt gtaattccag tgtttccggc
    13321 agggttaaac gaaagccaag gttccctgaa gaaagttgtt ctgacatctc gatcagctga
    13381 ttgatcggtt cttggacctt tctctctaaa aagcgacgat tctttttcgc atagaaaaaa
    13441 atggtactaa gaactccaag ttccactatt gttaatagaa tgagcgagcg gacaagtgtc
    13501 gtattttttt tgcttgctaa gtcgatctcc acacgaacaa aatcttgtaa gatatctgac
    13561 agcagttgag tgacagaatc tacctgattg atgatctctt cattggtatc cataaaatct
    13621 tttgccaaga tattattgat cagctcgttt tgataatctt ccagtgtatc gatcgtacgt
    13681 agcgatacat ctaacgtact aatttcttta agtgtatttg tattattttt gatctctcga
    13741 atatcattcc taagttcttc taggatattt tcctcattga actcatctac ggttacttgg
    13801 ccagaaatca agtcccacat actttccaaa actttttgat caaccttcaa agcaattgca
    13861 tctgcttttt ggatatttgc gatcgtctgt tgataaatca aaagctgttg cgaatataac
    13921 agtatgctca gtagcaaagg gataacagca aatagaagca tggctttgtt tgcttgggca
    13981 aaaatctgtt ttatgcttaa attttttgtc atgatacttc tccagtcaat aatttctggt
    14041 tgggatgtta gctatattct gctgggaaaa gacagtctcg gaaataaaaa tatctttagg
    14101 taatgttttt cctgtttgaa tatttccaaa ataacgagta acggtattgg ctacatacca
    14161 gccgacatca ggtttacact cgactacaca attgacgatt cctgagcgta gcttctcgat
    14221 catatcttct tgtccatcaa tagttacaat gatcaaatct tctccaggtt tgattgatgt
    14281 ttcttttatc gcagctaaag caccgtgagt catttcatca ctatggctgt ataatacatc
    14341 gatttcggat aaatcatttg tttctaaata tcgtttcatc tggtcttttc ctttcatccg
    14401 aataaaatca ccgacaattc ggtgaacaat agtcatcgga ggtttatggt cgatggttcc
    14461 atcttttttt agggttggtt tacgattgat tgattccaaa aatccttcgg agcgtagctt
    14521 ggtcggtgtg gagttctctg aaccagcaag ttctaggatt ctgatttcct gtttctttga
    14581 atttgtgaag tgattgctga caaataaacc cgcccgattt ccttctgctt tgaaactggg
    14641 gccaatgtgg gtgacgaaaa gctcgggatc attcgtttga atatgtctgt cgacaagtat
    14701 gacagggatc cctgctcgct ttgcttcttt taatacagag tcccacccgg attcttgcaa
    14761 aggagtaaaa atgatcatat ccactttata ggcaatgaac gtccgtatat cttggatttg
    14821 ccgttcctga ttcatgtagc catttcggta gagaacttgg taatcttctt tttcaagcgc
    14881 agtgataatt gattcagtat gctttttccg ccagctactc tccgttcctg attgcgaaaa
    14941 accaacgact agtgggtcag gtgcttcctt tgtattaggt gtacacccca tcatttggta
    15001 agcgattaca acaagggcta aaaaaaataa aacacgtttc actttaaatc actccatctg
    15061 gatttgttag tactatctta tcagataaaa gggaaaatca aagtcaaaag gaatcaatcg
    15121 aaaaaaatcc aagaaactta aaaaaatcca agacaaaaaa agtacaccta aaaaatttaa
    15181 agtaaatccc ataaaaaaat caatgttaac gctttcttta ttagctatac tcggtgtatc
    15241 aattgaaatg ggggataaaa aatgaaaaac aaatttagaa agattgttgg gactatcgct
    15301 attttgaccg caagcattct attaagtgcg tgtggcaatt caggatctgc agatgatagt
    15361 acggggtatg taggaatcgc tatgccgacc aaatcagcag aaagatggat cgctgatggg
    15421 aacaacatgg tctcggaatt agaaaaactg ggttataaaa cagatcttca atatggagaa
    15481 gataaagtag aaaatcaagt agcacaaata gaaaatatga ttacaaaagg ggtagataca
    15541 cttgtgattg cttctatcga tggttcggct ttaactgatg tgttagctaa agcaaaagaa
    15601 gcagatatca aggtgatcgc ctatgatcgt ttactgatga attcagaaaa tgtcgattat
    15661 tacgcgactt ttgataattt tggagtaggt gtatcgcaag cagcttatat tgaagagcac
    15721 ttaggattga aagaaggaaa aggaccattc acgattgaat tatttggtgg ctcgcctgac
    15781 gacaacaacg cactaatcaa ttacaacgga gtgatgtctg ttttacaacc atatatggac
    15841 aacggtcagt tggtcgtgcc atctggacag acgagtttca gtcaaatcgc tacgcttcgc
    15901 tgggatggtt ctacggcgca agcaaggatg gataatctgt taagcgcaaa ttatactgac
    15961 caaacattgg atgctgtttt gtcgccatat gatccaataa gtttaggaat catttcgtca
    16021 ttaaaaggcg tggggtatgg gagtgaaagt aaacctttac ctgtgattac tggacaagat
    16081 gcgacagttg ctggcgttaa atcgatcatt gccggggaac aaacacaaac gatctttaaa
    16141 gacacacgta tcttagcaaa aaatacgatt gaaatgatca aagcaatcag tgatggagaa
    16201 gaggttccgg tcaatgatac tgaaacttat gataatgggg tgaaaactgt ccctacgtat
    16261 ttagcaaata cagtatctgt cgataaagat aattatcaag cagagctgat cgacaccgat
    16321 tattataaag aatccgatct gaaaaattaa gaggaaccaa acaaggaact aaagagatct
    16381 aagtcagaga gaggataaga attatggcgg attgtatttt aaaaatggca gatatcacca
    16441 aaaaattttc tggaatccga gcgttagatt gcgtgaatct gatgattgaa cgtggggaaa
    16501 tccatgcatt gtgcggagaa aacggagcgg gaaaatcaac attgatgaat gtcttgagcg
    16561 gcttgtatcc atatggctcc tatgacgggg aaatctttta tgatggaaag ctgtgtcaat
    16621 ttaaagattt gaaagatagt gaagaaaaag gcatcgtcat cattcatcaa gaattagcac
    16681 taagtcctta tctttcaata aaagagaata tttttctcgg aaatgaacaa gcagaaagag
    16741 gcgtgattga ttgggaactg acagaaaaga aaacatatga actgctgaat aaagtcggtt
    16801 tgtctatcga cccgaatctt ctagtatccc agattggcgt agggcaacag caactagttg
    16861 aaatagctaa agcattatca aaatccgtcc gtctgttgat tttggatgag cctaccgctg
    16921 cattgaatga agaagaaagc gcgaatttgc tgagattgat ccgagagttg aaagagcaag
    16981 gtgtaacctc catcattatt tcacataagc tgaatgaaat cgtggatatc gcagatcgaa
    17041 tcaccatttt acgggatgga cagacgattg agacactaga aaatgaaatg atcagcgaag
    17101 agcatattat tcgcgggatg gtgggcagag atttagttga tcgttatcct gaaagaaaac
    17161 cggatattgg agaagtttat tttaaagtag aagactggac ggtctatcac cctcttgatc
    17221 acgagcgaat cataaacgac cacatcaact tgcatttgaa aaaaggagag attgtaggaa
    17281 tagctggact gatgggagcg ggcagaactg aattcgctat gagtctattt ggacattctt
    17341 atggaagcaa gatatccgga cgtatattaa aggaaggggc agagatatca gttaagaccg
    17401 ttccttcagc cattgcaaac ggactcgctt atgtctcgga ggaccgaaaa tctttaggac
    17461 tgaatctgct gatggatatt cgggaaaacg tgacgctgag cagtttggat aaaataagtc
    17521 aaaaaggagt attagataaa gaaaaagaag taactgaggc agaaaatttc cggaaaaaga
    17581 tgcgtataaa aacaaactcc atccatcaaa ttgtcagtag tcttagtggt gggaatcaac
    17641 aaaaagtcgt tttatccaag tggctgatga cacaacccga tattcttttt ttggatgagc
    17701 caacccgtgg gatagatgtt ggggcaaaat atgagatcta cacgattatc gaagaaatgg
    17761 cagcaaatgg caaagctgtc tgtcttatct cttctgaact tcctgaaata ttagggatgt
    17821 gtgatcgaat ctatacgatg aacgaaggac gtattacagg agaagtctcg cgagaagatg
    17881 cagatcagga agtcttgatg aaattaatga cgaaagaaga ggtgggttaa gttggaacag
    17941 gcaaaaaatc ttacggataa aaaagagaaa aaactaaatt tgaaaagtag agcagctgat
    18001 ctttttggtc gctacagcat ggtgatcatt ttagcgggac tacttattgc gtttcaattg
    18061 atgacggatg gtattttctg gcgtccattg aatataacta accttgttct gcaaaatagc
    18121 catatattag tactagctgc agggatgtta ttagttgtgc tcttaggtca tgtggatttg
    18181 tcggtagggt cagtgatggc gtttgttggt gcaattgcgg gcatgatgat ggtcaatcgc
    18241 caaatgaacc cgtggatgac agttatcgca tgtcttgcag taggcgggct gatcggcgca
    18301 tggcaaggtt tttgggtagc ttatgcaggg ataccggcgt ttatcgtgac tttagcagga
    18361 ttgctgatgt ttcgtggact gacacaagtt gttctaggag gccaatctct agcacctttt
    18421 ccagctgttt tccaaaaaat gtctacgggt tatttaccag acttaagcga aggaagcagc
    18481 cttcacttct tgacattgat tttaggaatg atcatcacga tcgtgttagt tgttggtcaa
    18541 tggcgaacaa gaaatcgtcg gaaaaatcat ttatttgaag tggaatctcc aacagctttc
    18601 ttggtaaagg cggtatttac agcagtcgtt gtactaggaa taacttatat ctttgcctct
    18661 tatcaagggt atccgattat tttgatcatt cttggtttga tcgttgcagc atatgggttt
    18721 ttgacaaata aaacagtagc aggtcgacag atttatgcga cgggcggaaa tcgaaaagcg
    18781 gctgaactat cagggatcaa aacaaagcga atcacgttct gggtattcgt caacatggga
    18841 gtcatggcag ctttagctgg attagtttta gctgctcgat tgaatgcggc aacacctcaa
    18901 gcaggtacat cgatggaact ggatgcaatg gcggcagtat attttggtgg tgcttcgact
    18961 tccggcggca ttggaacaat catgggtgcg attgtcggtg gcttggtcat gggtgtattg
    19021 aacaatggca tgtcgatttt aggcgtcggg gtagactggc agcaggcaat caaaggattg
    19081 atcttgcttt tagccgttgt gctagacttg tataataaga aaaaacgaac aacgtaggag
    19141 aacgccggtt attcagaaaa aagactgtga caagacattt gtcacagtct ttttttcgct
    19201 aaaagcgaac attcattgtt tcgtttgtat agactaaaaa taatatatgt ttttttacca
    19261 ttcgtataaa ttctcttttt gctcttgatt tttttgctta attgagttaa attacatagg
    19321 gcgctaaaaa aagccttgat taaattaata atgttcgtgt tttgctggat gtgaacatca
    19381 ggaggataat gtaatgaaag tatttgatta cgaagatatt caattaattc ctaataaatg
    19441 tatcgtaaat agccgttctg aatgtgatac aacggtcaca ttaggtaaac atacattcaa
    19501 aatgccagta gtgcctgcca acatgcagac gattattgat gaaacaatcg cagaattctt
    19561 agcggaaaat ggttatttct atatcatgca tcggtttgat gaagcagctc gaattccgtt
    19621 tatcaaaaaa atgaaaaagc gtggattgat tacctcgatc agtgttggcg tcaagaaaga
    19681 ggaatattcc tttatagaga agttggcaga agaatcgcta aatcctgatt atatcactat
    19741 cgacatcgcg catggacatg ctaactcagt tattgatatg attcagcata ttaaaaaata
    19801 tttgcctgaa acttttgtga ttgccggtaa tgttggtaca ccggaagccg tccgtgaatt
    19861 ggaaaatgcg ggcgcagatg caacaaaagt cgggattggt ccaggaaaag tatgtattac
    19921 aaaaatcaag acaggctttg ggactggagg ctggcaactg gcagctctta gatggtgtgc
    19981 gaaagcagct cgcaagccga tcatcgctga cggtgggatc agaacacatg gcgatattgc
    20041 aaaatctgtc cgttttggtg cgacaatggt catgattggt tcattgtttg ccggtcacga
    20101 ggaatctcct ggcgaaacaa aagtcgaaaa tggaatcgtg tataaagaat actttggttc
    20161 tgcttcagaa ttccaaaaag gcgaaaaacg aaatgttgaa ggaaagaaaa tctggatcca
    20221 acacaaagga tctttgaaag atacactagt ggaaatgcaa caagaccttc aatcatctat
    20281 ttcttatgct ggaggaagag atctggaagc cattcgaaaa gttgactatg tcatcgtgaa
    20341 aaattcgatt tttaatggag acacaatcta aaaaataaat aaaatattag gatgttgtga
    20401 aaaagttgtc ctgcattaag taataagaac agccaaacaa aaatagttct ttatatgttt
    20461 tgtttggctg gaattattat cgcagataca agcttttgaa catcattatt cagaataagg
    20521 gactgtgata agatttttgt cacagtccct tatgttttgc aaaaaaagtt tttctttttt
    20581 ctcgtaaatt cggtaaaatc aaaagaaaaa atagacaata gaaaagagaa gcttatgtgg
    20641 gaaaaagtag agaaaaaaca aggaccgatt tatcgtcaaa ttatggaaca gattatgaga
    20701 gaaatcgaaa acggaaggat caatcctgga agtcaacttg aatcagaacg aaaactaggg
    20761 caactttttg gtgtgaatcg ttccaccgtt gtccatgcat tggaagagct tagggagctt
    20821 ggggtactaa cgagcaaacg tggcagcgga tggtttgtta atcagactga atgggggaaa
    20881 ttcgctgttc caagaatcga ttggcggcaa atgatctctc ctcgttatga gcagacggac
    20941 tggtatgagc aaaaaataag agaagcaaag cagatccaaa aaacatcgtt tcttgattta
    21001 tacgctagcg agatgccaga tgagcttttg ccgaattttg agtttccttc ttattctctt
    21061 gaagaaatct tagcggaaga aaaagaaatg gatcttttag gctatactcc gttacgtaac
    21121 aaaatcaaac ggtatttgga aagtaaattt gaattggcat tttcccccaa ccagcttctt
    21181 gtgactggtg gcggtcagca agcaatcttt ttgattttac aaacgattct ttcagctgga
    21241 gaggggatag ctgtagagtc accttctttt ttttaccgat tacctctttt caaagcttca
    21301 ggaacgcgtt tatttgggat accgatggat gaagaaggga tcgacttaga aaaactgaaa
    21361 gaatcgatcc ataaaaataa attgaaagca gtgcttatca acccgaattt tcaaaaccct
    21421 acaggaaaag tgatgagtca aaaacgacga gaaaagctag tagaattgtg tcgacagtat
    21481 cggcttccaa ttattgaaga cgatgtattt acggatcttt catttctaaa ccaagaagaa
    21541 gtccttccta tacgaacgat tgatccggat aatgtgttat acgtcggttc gttatctagg
    21601 gttttaggaa aaacaacgaa aatcggttgg gtgataggac ctgagacttt tatctcgcaa
    21661 ctagctcaag cgcaagaaat gatggagttt tcgatgaata tccttacaca aatcacagtg
    21721 gctaatgtat ttaatgaagc aattgatttg aaaatgcaag atgtaagaaa aaagttgaaa
    21781 gaaaaaagcc gactgataag cgcttgggga gattcacaat cgtttttcaa gcttcattca
    21841 attttaggtg gttactatgc ctggattact tgggatggga aaaaactaga ccccgaacta
    21901 gcagaaaaag tagtagatat gggtcttgga attgcaccaa gtctactttt tgggcaagat
    21961 ataaatggaa tcagaatcaa ttacagtagg ctggatgaaa accaaatacc gttgttttat
    22021 tcaaagatga aaattctaga aaagtggtta a
//
LOCUS       DAYYCY010000028        22031 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000028 DAYYCY010000000
VERSION     DAYYCY010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22031
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.028"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            367..555
                     /locus_tag="ACHAZB_000885"
     CDS             367..555
                     /locus_tag="ACHAZB_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8613576.1"
                     /translation="MELIVFTNNGQTYHFYEVENFKPTTTGFSFIYTGKATGVTRSAT
                     FNNTSTAGYALAEVQNES"
     gene            545..805
                     /locus_tag="ACHAZB_000886"
     CDS             545..805
                     /locus_tag="ACHAZB_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6275 family protein"
                     /protein_id="HFM8613577.1"
                     /translation="MNHEKFIEKCKAIVRERIENEIADLSGAVPEFSIFIVWSCKTLQ
                     NSKALVSASLKGAPYFEITLNGDKGEIYVDTYLKKSNECIKV"
     gene            846..1073
                     /locus_tag="ACHAZB_000887"
     CDS             846..1073
                     /locus_tag="ACHAZB_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8613578.1"
                     /translation="MRNYWYVSLTNEYPRTIGDCSVRVVRSVQIKGKYSIVEMLREAT
                     PNEVDKCKLIYCGHGYWKDEYIQYNIERYIK"
     gene            1138..1710
                     /locus_tag="ACHAZB_000888"
     CDS             1138..1710
                     /locus_tag="ACHAZB_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8613579.1"
                     /translation="MKLKDYIREGYNVVTAPNLAYKIQEDYPNALVFTDRALDAIPIG
                     KLLVDRFYSNNPAVLSAKPLQNAYAIERFTCEFTGYETVVPMTGETGRKVIQQIKKRI
                     AELTLDDGSNTNLLEIKLRDTDSVPEVYYKGQRMDEMPIGLVDITYHWDTEEYECQVP
                     KGANDITIEYYDSHNNKYLDRKIIGHKRDM"
     gene            1711..2022
                     /locus_tag="ACHAZB_000889"
     CDS             1711..2022
                     /locus_tag="ACHAZB_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFM8613580.1"
                     /translation="MIDVSSKQARARFYGSTEWRKLRSQCLERDHYECQWCKAEGRLT
                     TQHDSILEVDHIKELEYHPDLALDINNLRTLCKDCHNKRHNRMKYRGQQKKRKWDDEW
                     W"
     gene            2013..2273
                     /locus_tag="ACHAZB_000890"
     CDS             2013..2273
                     /locus_tag="ACHAZB_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8613581.1"
                     /translation="MVVRKTANRDDVKRAEAIAMVDKAIDKLVLFKSQLIEGTTTVED
                     ISINNRLIGNANEVTGTFNINVDIDYTLSPFAHIVESNEQDG"
     gene            2434..2793
                     /locus_tag="ACHAZB_000891"
     CDS             2434..2793
                     /locus_tag="ACHAZB_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8613582.1"
                     /translation="MADLQERNRLVSAEERRLNKLFKDIDKDKKKVVSGLITQAARLK
                     ILLDEMWIDISEKGDYELFSQSENQKPYERERPVAKQYNSRDQAYQRVIKQLTDYLPE
                     EKREPTKNAALDGSDLL"
     gene            2790..4433
                     /locus_tag="ACHAZB_000892"
     CDS             2790..4433
                     /locus_tag="ACHAZB_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFM8613583.1"
                     /translation="MTTLKPFYFDEYVDLYEKGVIPFNKERIQLITYLKNEVLVRDDI
                     YFDEEMIQKFIKYTEKNFFPLAKYQKFITPFIFCYQKEDDEVFFDEILNSIARGGGKN
                     GFMSARDSFFISPLYGVRNYDVTITANSEKQGKVSFKEVYEMVQMRKLEKQFYLTKMA
                     IVNNVTNSIFSYRTNNPKTMDSARDGCLEFDEIHQFESSDLVDIQRSGLGKIKHPRTF
                     YNGTNGHVREGFYDKMLERAQKIFSGENKNDRLFPFICKLDNIDEMDNPKMWSKANPM
                     FEEDSAYSKRLFSTVMKEYKKLEDEPSGRREFVVKRMNFTEGDAESDITTHEKLMATK
                     QELPNLNGRSCVAGFDYASIRDFATVGLLFKIDDKFIWQQHSFARKKFLDTFKLKAPI
                     KDWEKKGLVTIVDEPSIDPRHLIDWLNEKRKIHQIEIVCADGFRMDLLKPLLEKESYN
                     FEFIRNIRGVQSKVAPIIEDGFANERFIFGDDPMMRWYTNNSFIKEDKLGNKTFLKKE
                     PVRRKTDGFHAFLAALYKREEITDMDYEEAFDMLDEIEF"
     gene            4468..5550
                     /locus_tag="ACHAZB_000893"
     CDS             4468..5550
                     /locus_tag="ACHAZB_000893"
                     /inference="COORDINATES: protein motif:HMM:NF028182.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative phage abortive infection protein"
                     /protein_id="HFM8613584.1"
                     /translation="MKKRYFLFVTIFIIIAICVLTVFLPQITEYLNYRIFTTKVDDSV
                     TSLSRLEFIKICASVFGPILTVMVFVNTLNMQKKTEQKQNELEVKQAKAENERAKKEL
                     LTQIDREFYFLLNMFIEIQKDQNAKQAVIALQKLAIFNEKGFNQDYAFTSKDLSDSEI
                     GSTIEQSGPLKGTVEYIKFETFDVSKSMGNYQTKTKDVATSLHDQKVAILNEQYEKMQ
                     IYLGRYFKMFHRIVKTLNEYYDDYNDFDVKRYTKYIGTLRTQISPAEFQVILFNSLYI
                     KRGFGLGIQLIGSGFFGDDFDFETNQHFETSINEQWFLSLSTVDSDNSIKRQKLSEYI
                     KELGSVEYKKIANFESLYKLFNETKL"
     gene            5594..6766
                     /locus_tag="ACHAZB_000894"
     CDS             5594..6766
                     /locus_tag="ACHAZB_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFM8613585.1"
                     /translation="MSLFDIFKQSIRNEEPSEWIPDFVYGEEVSARAYLKIMAKNTVL
                     DFVARTMSTLEVKFKNKDGTSDWDYILNVRPNSDMSAATFWQKFFYRLLDDNEVLVIF
                     TDDNQLLIADDFSRTEYAVYDDVFTNVMVKNFVFERSWNMSDVIYMEYNNEELERFTR
                     GLFEDYAALFGRILEVAMRNNQIRGSVSIDSTGTANDEKGKDGKTRSQRLQEYIDRIW
                     NSFKTNSVAIVPKVKGFEYEEYTNKQGVSNQSLDELNKMKSSLIDDVANAIGVPTALI
                     YGEKAELDSNIKAFRKLCIAPLVKKLQDELVAKIISRQEYKNGERIEVKKVLQKDLLE
                     YATQIDKITSGGAFYIDEVRDEVDYDELPNNEGKIRIRTKNYEETVKGGENDNAES"
     gene            6753..7457
                     /locus_tag="ACHAZB_000895"
     CDS             6753..7457
                     /locus_tag="ACHAZB_000895"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFM8613586.1"
                     /translation="MPKAKKVPFQFTNEIQNGKHILTLSGNVQKKYWRDDDVINAKDI
                     RDSLDTVTDDIVIKLNSPGGDVFEGIEIYNYLKDHPSNITVEVTGLAASAATFIVAGA
                     NDVIMNVGTSLMIHEASTFAWGNKQDIQKTLNALETIDDSILAIYSDKTGQSVDKLRE
                     WMNEEKWFTADEAVKYGFANSVKREEPQNEPQNIAAMIQDAVAVAIANLKEPVTNKIE
                     QESKQKSLIARLRKGE"
     gene            7462..8622
                     /locus_tag="ACHAZB_000896"
     CDS             7462..8622
                     /locus_tag="ACHAZB_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFM8613587.1"
                     /translation="MLKITDKTADAKKAFNAISSKEDATPEQINAALEEYVTAIAEDA
                     GKQVRAEYEELKNVTDNRVLEARGIPTLTAEETKFYNEAVKSGGFDSDVVWPETILER
                     VFENLQNDHPILGLINFTPTVGRVKVIRARRKGVAVFGPLHKDLEGQLDAEFGATEFV
                     QLALTAFFLISNDTLDLGPRWIDRFINLSLSEAVRDVWAKKIITGSGKDEPIGLLKDL
                     DGAVTSGVYPDKASAGTLTFAKEKIVLELAGVMKKLSKYTYKVDGDDEGEVKYRSVAG
                     RVYLIVNPVDYWDIVARVTFANLNNVYGTTMPFIAVDHIIESVDVPESKCIAYVDKEY
                     EATQSRAEKVYVYKETFAMKRATLYAVDMLGNGYPTNNDAAQVYDLDFTAGE"
     gene            8698..8976
                     /locus_tag="ACHAZB_000897"
     CDS             8698..8976
                     /locus_tag="ACHAZB_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HFM8613588.1"
                     /translation="MNTEEFIKEYKARFRIFHTSEDEDIGKQLESGFADIKSLIGSFD
                     PSTYEKGKELVYERTRYLRNESLEYFYDNFQTMIMDASIDLAGAGYAN"
     gene            8957..9328
                     /locus_tag="ACHAZB_000898"
     CDS             8957..9328
                     /locus_tag="ACHAZB_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HFM8613589.1"
                     /translation="MLDMPIKSNYKKPKIVAGDLNTPVTFFEFQPNKGPEPGDEEKKE
                     LYFCTALVYNPSMKDRDILGTKEGVTIKIRDPHQSYIPTNKHKVRILDYRYGDKVWEI
                     IDVSPDLEENSIVKIVLELIT"
     gene            9344..9748
                     /locus_tag="ACHAZB_000899"
     CDS             9344..9748
                     /locus_tag="ACHAZB_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8613590.1"
                     /translation="MSNVKFEGVQETIQALEKKYGEKKTKTLTKKAINVGAEKVEKQL
                     QTDMTVFKDKGYTIAEVVRKNATYRDYKAEAEIGWNGPHQRYRIIHLNEWGYTRKGKQ
                     IRPRGFGVITKSLKNSELLYFDAVGEEVKKSL"
     gene            9745..10155
                     /locus_tag="ACHAZB_000900"
     CDS             9745..10155
                     /locus_tag="ACHAZB_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8613591.1"
                     /translation="MKDMLNIIYEALCLNEYIHSMTYNENTEQYRIKFYEQPETADKT
                     GPMITIRPVDTPNEAYHGSNKELSVEHMIQIDVESKYRATCKQIQYEIKKEMKKLGFG
                     QISGQGLDEYFTETNRFVDARRYEGNTKIYDTEY"
     gene            10216..10803
                     /locus_tag="ACHAZB_000901"
     CDS             10216..10803
                     /locus_tag="ACHAZB_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HFM8613592.1"
                     /translation="MTLVGFKKMTIGVFDSTGKIPAANLYVIEGEQNKGATVSAEISG
                     LSKEPSKVYGSNIAYYVSQKGTGDVSATFGLLDLPTEVNDKILGYKTDTNKISFLGED
                     TEPPYCAILMESEDLNGDTAMLAMFKGKFSRESINLNTTTNEAFEPEAEEYVFSAIAN
                     DVEGDAKGQTVAKYVGDEEASITGLKEMVFPAAGE"
     gene            10876..11343
                     /locus_tag="ACHAZB_000902"
     CDS             10876..11343
                     /locus_tag="ACHAZB_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HFM8613593.1"
                     /translation="MPDTFKIYKKNGTEFTKVAEGESPLSITGIAANTQVAKGDYQAT
                     RVVGGQESVKVDIPAFTTLPISVTGVTLDKTTAEVEQGGTLKLTPTVTPANATDKTGS
                     WSSSNTAVATVTGGNVTVKTDAEVGGTTEVSFTTTDGGKVAKCTITVIEKAAG"
     gene            11389..11697
                     /locus_tag="ACHAZB_000903"
     CDS             11389..11697
                     /locus_tag="ACHAZB_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8613594.1"
                     /translation="MAKVRIELKEESGKKVYENIDTTGKDYRKALETIKKLNEDGVMI
                     WNRLDIYLDFAVGIFRDSKLTAEQILDGLPSEKVMSTLDDILGEVMGIESNPDPDAKK
                     "
     gene            11892..18626
                     /locus_tag="ACHAZB_000904"
     CDS             11892..18626
                     /locus_tag="ACHAZB_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFM8613595.1"
                     /translation="MANGKPLGNMIIKLDLDSSAFSKGLAGAKNAVNHQMKAMKSQMQ
                     VMNSSGNSLGALQAKYNGLGGVLQANEKQVELLTKAYKDSFDSNGNATASTAKYANQL
                     NQATARSASYESQMKTTVGQIARMKVETEGFTGKLKANSDVLINSGKKIEAFGKGVSG
                     IGSALTVGVTAPIVAGATAVTSAAISWESAFAGVKKTNDEVVDSNGNVVYSYSDLEKG
                     LRNLATQLPGSHQEIANVAEAAGQLGIKTENVVSFTKTMIDMGESTNMSAETAATSLA
                     RFANITQMSQKDFDKLGSVIVDLGNNFATTESEITEMGLRLAGAGKQIGMSQGEILGF
                     ATALSSVGVEAEAGGSAFSKVMIQMQLAVEKGFGAFDQLKQMAEEQGVPWVNLVNAVR
                     DGGKSLKAVSEQMGFTSSDLKKMYKEADNSKSSLENFANVAGVTSEQFQKLFKSDPSE
                     AIIKFIQGLKDSEKHGTSAIKVLDDMDIKEVRLRDSLLRAANASDIFKGAIEKGNGAW
                     KQNTALTNEANKRYETTESKLKILKNEVIDTAIDLGGPFVDALRDGLNVSKPLIKNLG
                     NMAKAFSAMSDDQQRNIIKWVGLAAAAGPALKLLGTGTSVIGKVTSTTGRLTKSLVDL
                     AAKAAEKKAMDAFASSVTTAGAAAAKTAGAGGIGGLVPMMGKTASAASTAAGASGIGA
                     MTTSLGLLGPALLGIVGVGGALAVGYGAWKLFGEEAWNSSQRVKQWGTDVGEEIDSTL
                     DGVQKNIEGANGQFGLLKEGFTVDDANNMAANFATAGTSLETSLTGRIKALDDALKGL
                     PQSVQDAMKEVAESEEETLGQSLSKIKDNNEEIKKIRERAANQNRDLTVSELQQIQDL
                     MKGTSQAYVDTLDVSAKDRKKILSAMNGDVAQATEEEAKTWLQSLAKQKAATTENYRQ
                     MLEEKKAALKEKGVSKEFIDALEDEMNDYIKTTNQGFDSQIAAITNKYPQLINEVSLS
                     NGQLFSKTAAAMDNTGQYTKVFLESNEKILAQAKKTSDEIADNAEKNAEQLGMVADEA
                     THAGSTWNGLVLDEKTGKVKTNVQEVVNEATKDFTKWNELKPVVHDAKLTTNAKDVIS
                     IAAVQNGYWDDMEWKEKEALLEDECSKNVYKALENAGKWNELDIPSKEAILTSNTPEV
                     MGETLMNLGLWDTYKLQVKDLDLNAYKVYDAIDGSEEKLNSWAQLDNPTKELLLDNSD
                     FSTKLFNSEEMFKRWNQLSSNAKFLSANNTKFTEVLLSSDRMWNQWVNLPDNEKRLLA
                     DNEDLATTVLSSEEIYGRWTQLPDNQKYMLADNTDLANKIFASEESWNAWKAIPDTVK
                     NLLGNNIDLLAKVKDGTVSVEEYNKVMPLLKTLYGDNVNLKEAISGAKSSIENYNKNI
                     FPSDKTAVGHDEASQAAKDAFEAFNIFQTKIPDKITKTVSADFIGPMPKNAKGTNFHP
                     GGAAMVNDQKGPTYEELITLPSGETFIPKGRNVVLDLPRGSKVLNATKTKRLVPKYAD
                     GIGNITTVSSTLNLDALILAINELTTVLRTQLMAGTTDAKDTKLSDETKVADPIIPDS
                     LSEKSDQYLGIGAQWLTNLMNGWNSAVPQYMNSEMVFITNYLNALKLQNNPNYTQGAT
                     WNKNLLNGWNSLTGTFIATINSFCNQAMVTLRNYNTPMYNNGRTWQQNNLNGWNSLYG
                     SFIARVNQLGNDSINNLRSKSGGFYNAGTFLLQSLINGMNSLGNSLSTTMNNVANTMV
                     GGIGKGVNGVISGVNYVLKEVESSKNIGNWPIPQYAKGTDGHPGGLAMINDQKGLVHE
                     EYVQMPDGRSFIAKGRDLLVNLPKGAQVLNASLTKKLKERLNIPHYEKGIGNLDIVDL
                     LDDEKRMLEFLTSKVDFSGINEPWLDMTKSGTSLMSKAANTMLQSKLSEFFTHGNFDG
                     AVNANGVYQYLVDVAQKVMGKFPGLTVTSGYRAGDAYYHGKRQAIDLAYPGISGDPRY
                     TAAANYAFEKFPSKIAYVITNGRVRDRMGLSGTRSSGQWTNWPDGDHFDHIHLNGSMG
                     SGDIFNGGVGGSGVARWRSYVSKALKMNGLPATAAYINAWMSQIQTESGGNEKAIGGN
                     DGLAEGNATGLLQTKPGTFAANAFPGHGNIMNGFDNMLAAINYAKKRYGVAGMLQVIG
                     KGHGYANGGLITKDGLYRAGEGNKPEMVIPLTRKTRAIELMGQALAFLSGSNKKETKS
                     EINSVDNRHILQAIEKQSRMTNDLLSLLLSFFKSNNRSDKELALDIQKILIRRT"
     gene            18630..19502
                     /locus_tag="ACHAZB_000905"
     CDS             18630..19502
                     /locus_tag="ACHAZB_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage distal tail protein domain-containing
                     protein"
                     /protein_id="HFM8613596.1"
                     /translation="MRTVILRNKLNEEIDLSTEDYFATEMSNLGFEVTKEHIGQWGSF
                     REIGESIELSEFQSSVMISVHGFREQQLYGSLVEFLANGPFELEFSFDSETMIRKCSL
                     KSLSKTEIDSQSSLLTDTLNLYFTSSWYSIKREKLIQNSTSLNTRGKVYSFVRPYVYT
                     KNVWEKKGVFKFSNNSVYLTNSTERMSPLKIRIIGECSNPYWEVIQNSKVVASDGYFI
                     NMKDTQILEVSSLLEDQTAILKDVGGVESSVYQQQDFTKSNFVQAPLGEFSVVFHVGH
                     ANVEIELYEERDLF"
     gene            19499..20548
                     /locus_tag="ACHAZB_000906"
     CDS             19499..20548
                     /locus_tag="ACHAZB_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8613597.1"
                     /translation="MILAITLFHRDMNLYNERLISTEFEFGTDEIGEEVSNFITDKYV
                     HVKSGDFLLAKFIPSGKIAYFGVISSQEDKKINCKGLLSLADSEIPTVKISGANYEEH
                     IRRLIEYYLLNDPTKQLKNILEVKTESVTSHSYQAVDTNKRRLNAYIINGFKKYNVKW
                     YVKEIKGKKIYTGIRAVDDSIYIKDNSSEFNDWDVFVQSPGPGNENKLLIVDKAMKDI
                     ENPLVLSTWYLDDENNLTQDSTNKNIALPTVNLVNIYDQTQEDRPSYEEVAKSELKGN
                     AYSHEIKVNVVLNARNINVENIETGMLATIVYKEQTYKSVLTAWRISSSKKNIELTFG
                     NVRSRFMDYFEDNGG"
     gene            20552..21376
                     /locus_tag="ACHAZB_000907"
     CDS             20552..21376
                     /locus_tag="ACHAZB_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8613598.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVNSTGSTVSISKISDSYELTLS
                     KGEILWRGTALMHDTQTVQPSKKIWQTVSGFLLVWLPYENGQAIEDRYVTTPFYKERI
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            21394..>22031
                     /locus_tag="ACHAZB_000908"
     CDS             21394..>22031
                     /locus_tag="ACHAZB_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HFM8613599.1"
                     /translation="MANLEIKLSANKRQPYLRHRVVGRVGDGGLTTINVQLLEEDEIT
                     PFVINLNGTLKFVGEVSNGNYTEGEPEIIDSTNGLISYTFTKSNFSTSHQFKQAYFEY
                     VDPNGKKVTFQNFIIDVLERVDINSEQAKYYISSLEKLQSEMQTTFNQFISDKQVQYD
                     QIYSKYNELVRLINESDKQVNDRIDKANNRIDQTNQQIGDLGKLKKMYSNSI"
BASE COUNT         7878 a         3297 c         4671 g         6185 t
ORIGIN      
        1 cttctaaatt gttcttatta tctatcatca gttgctgtct attgtcatta tgtcactgtg
       61 gtggaaaggt gtatcgctca tctaaaattt aggtgcaaac tgcaaggttc gattccttgc
      121 cagtgacttt ggtaaccgag gcatcggcgg tttaaaaata taggggtgcg caatttcgta
      181 cgcgttttgt gcatcttgta ggttgctatt acataattgg ttgggttaga ttgagttttg
      241 ggattcggta caaatgaatc gtcaaatgac tcaagcacag gatcggaaac gtccctgcct
      301 gtgcattaca tattagatca ctctttgagt ggtcttttta ttttaaaaaa gagaggattt
      361 tgaaaaatgg aattgattgt atttactaat aacggacaaa catatcattt ttatgaagtg
      421 gagaatttta agccaacaac aacaggattt agttttattt atactgggaa ggctacggga
      481 gttactcgtt cagctacgtt taacaataca tcaacagctg gttatgcgct tgcggaggta
      541 caaaatgaat catgagaagt ttatcgaaaa atgcaaggct attgtacgtg aaagaattga
      601 aaatgagatt gctgacctaa gtggagcagt gcctgagttt agtattttca tagtctggtc
      661 atgcaaaaca ctgcaaaata gcaaagcatt agtcagcgct agcttaaaag gagcaccgta
      721 ttttgaaatt acgttgaacg gggacaaagg tgaaatctat gtagatactt atctcaaaaa
      781 atcaaatgaa tgtatcaaag tctagcaggt gctaggcttt ttctttacat aaaggaggct
      841 gcataatgag aaactactgg tatgtatcac taactaatga atatcctcga accattggtg
      901 attgttcagt gcgtgttgtg cgttctgtac aaatcaaagg gaagtactct attgtcgaaa
      961 tgctaagaga agctacacca aacgaagtgg ataaatgcaa gctgatatat tgcggtcatg
     1021 gctattggaa agacgagtat atccaataca acattgagag gtacatcaaa tgaatgataa
     1081 ctatgattac atcaagttgt tagcaagtaa ttattagatg tttttaggag gaaaactatg
     1141 aaattaaaag attatatcag agaagggtat aacgttgtaa ctgcacccaa tctagcttac
     1201 aaaattcaag aagattatcc caatgcttta gtgtttactg atagagcttt agatgccatt
     1261 cctataggga agttattagt agatcgtttt tatagcaata atccagctgt tcttagtgct
     1321 aagccacttc aaaacgctta tgctattgaa cgctttactt gcgagtttac tggatatgag
     1381 acggtagtac caatgacagg tgagacagga agaaaagtaa ttcaacagat caaaaagagg
     1441 attgctgaat tgacgttaga tgatggaagt aatacgaacc ttctagaaat caaactaaga
     1501 gatactgact cagtaccaga agtttattat aaaggtcaac gaatggatga gatgccgata
     1561 ggtcttgtcg atattactta tcattgggat acggaagaat atgaatgcca agtaccaaaa
     1621 ggagctaacg acatcactat tgaatattat gattcacata ataacaagta cttagataga
     1681 aaaatcattg ggcacaagag agatatgtaa atgattgatg tttcaagtaa acaagcaaga
     1741 gctaggttct atggatcaac tgagtggagg aaacttagaa gtcaatgctt agagcgagat
     1801 cattatgagt gccaatggtg taaagcagag ggtagactaa caactcaaca tgattcaatt
     1861 cttgaagtgg atcatataaa ggagctggag tatcatccgg atttagctct agatataaac
     1921 aacctaagaa ccttatgcaa agattgccat aacaaaaggc acaaccgtat gaaatatcgt
     1981 ggccaacaaa agaaaagaaa gtgggatgat gaatggtggt aagaaaaaca gcaaacagag
     2041 atgatgtcaa acgagcagaa gcaatcgcta tggttgataa ggctattgat aagttggttc
     2101 tatttaagag tcagctcatt gaaggaacaa caactgtaga ggatatatct atcaacaata
     2161 gactaatagg taacgctaat gaagtaacag ggacatttaa tattaatgta gacattgatt
     2221 ataccttgag tccatttgca catattgtag aaagcaacga gcaagacggc tgataaatca
     2281 gtataaagcg aagatatata gagtgaaata cccccggtca aaagattttg agaagaaata
     2341 aaatttttgg gaaccggtgg atggggtcaa ctctgcaaat aaatgatgct attttcacac
     2401 gaccccctcc ccacgtagaa aggaagtgag ttgatggctg atctgcaaga gagaaatagg
     2461 cttgtttctg ctgaagaaag gcgtctaaat aaattgttta aggatattga taaggataaa
     2521 aagaaggtcg tttctggttt gattacgcaa gcggcacgct taaaaattct tcttgatgaa
     2581 atgtggattg atatttccga aaaaggtgat tatgagcttt tctctcagtc agaaaatcaa
     2641 aaaccttacg agcgggaacg accagttgct aaacagtata attcgagaga ccaagcatat
     2701 caacgtgtaa ttaagcaact tactgactat ttgccagaag aaaagcgcga acctacaaaa
     2761 aatgcagcgc tagatggtag tgatttgctg tgacaacatt aaagccattc tattttgatg
     2821 aatacgtaga tttatacgaa aaaggggtaa tcccttttaa taaagaaaga attcagttaa
     2881 ttacttattt aaaaaacgaa gtgctagttc gggatgatat ctattttgat gaagaaatga
     2941 ttcaaaagtt tatcaagtac actgaaaaaa atttttttcc tttagctaaa tatcaaaaat
     3001 ttatcacacc tttcattttc tgctatcaaa aagaggacga cgaggtgttt tttgatgaaa
     3061 ttctaaatag tattgcgcga ggcggcggga aaaatgggtt catgtcagca agagactcat
     3121 tttttattag ccctctctac ggtgtcagaa attatgatgt aacgattaca gcgaattctg
     3181 agaagcaggg aaaggtatct tttaaagaag tttatgaaat ggtacaaatg cgcaaactgg
     3241 aaaaacaatt ttatttgact aagatggcta tagtcaacaa tgtaactaat tcgattttta
     3301 gttataggac aaacaatcct aagactatgg atagtgcaag agatggctgc ttagaatttg
     3361 atgaaatcca tcagtttgaa tcttcagacc tagttgatat tcagcgtagt gggctaggga
     3421 aaataaaaca tcctcgaact ttttataatg gtacaaatgg tcatgttaga gaaggttttt
     3481 atgacaaaat gttggaacga gcacaaaaga tttttagtgg agagaacaag aatgatcgct
     3541 tgtttccctt tatttgtaaa ctggataaca ttgatgaaat ggataatcct aaaatgtggt
     3601 caaaggctaa tccaatgttc gaagaagatt cagcatattc aaaacgtctg ttttctacag
     3661 tgatgaaaga atataaaaaa ctagaagatg agccatcagg taggcgtgaa tttgttgtta
     3721 aacgaatgaa ttttacagaa ggtgatgcag aatcagatat tacgactcat gaaaaattaa
     3781 tggctactaa acaagaattg ccaaatttga atggtcgttc ttgtgtggct ggatttgatt
     3841 atgcaagtat tcgtgatttt gcaacagtgg gtttgctttt taaaattgat gataaattta
     3901 tctggcagca acatagtttt gctaggaaaa agtttctaga tacattcaaa ctgaaagcgc
     3961 ctattaagga ttgggagaaa aaaggtttag tgacaatcgt tgatgagcca tcaattgatc
     4021 ctcgtcattt aattgattgg ttaaatgaaa aaagaaaaat ccatcaaata gaaatcgttt
     4081 gtgccgacgg atttcgtatg gatttgctga aacctcttct agagaaagaa tcatataatt
     4141 ttgagtttat cagaaatata agaggtgtgc agtcaaaagt tgctcctatt attgaagatg
     4201 gttttgcaaa tgaacggttt atttttggtg atgatcctat gatgcgttgg tatacaaata
     4261 attcgtttat caaagaagac aaattaggga ataaaacatt tttgaaaaaa gagccagtaa
     4321 ggcgtaaaac agatgggttt catgcttttt tggcagcttt atataaacga gaagaaataa
     4381 ctgatatgga ttatgaggaa gcatttgata tgttggatga gattgaattt taaggtatac
     4441 tccttattga aaggggtagt tatagaagtg aaaaagagat attttctatt tgttacaata
     4501 tttataataa tagcaatttg tgttttgaca gtatttttac cacaaattac tgagtattta
     4561 aattatcgga tatttaccac aaaagtagat gatagcgtta ctagtttaag taggctagaa
     4621 tttataaaaa tatgtgcatc tgtgtttggt cctattttga ctgtaatggt atttgtgaat
     4681 actttaaata tgcagaaaaa aacagagcaa aagcaaaatg agcttgaagt aaagcaagct
     4741 aaggctgaga acgagagagc aaaaaaagag ctgttaacac agatagatcg tgagttctat
     4801 tttttattaa atatgtttat tgaaatacaa aaagatcaaa atgccaaaca agctgttatt
     4861 gctttacaga agttggcaat ttttaacgaa aagggtttta atcaagacta tgcatttact
     4921 tctaaagatt tatcagatag tgaaattggg agtacaattg aacaatcagg accgctgaag
     4981 ggcacagttg aatatataaa atttgaaaca tttgatgtaa gcaagtcgat ggggaattat
     5041 caaacaaaaa caaaagacgt tgctacaagt ttacacgatc agaaagtagc tatattgaat
     5101 gaacaatatg aaaagatgca aatatatctt ggacggtact tcaaaatgtt tcaccgaata
     5161 gttaaaacat taaatgagta ttatgacgat tataatgatt ttgatgtgaa gaggtataca
     5221 aaatatatag ggacattaag aacacaaatt tctccagctg aattccaagt gattttattc
     5281 aattctctat atataaaacg aggttttggc ttaggaattc aacttattgg aagtggtttt
     5341 tttggagatg attttgattt tgaaactaat caacattttg aaacaagtat caatgaacag
     5401 tggtttctat cactatcaac tgttgacagt gataactcta taaagcgaca aaaattatct
     5461 gaatatatta aagaattagg atccgtagaa tataaaaaaa tagctaattt tgaaagttta
     5521 tataaactct ttaatgaaac taagctctag aaatagggct tttttatttt tctttgaaag
     5581 gtggtgaaaa catgtgagct tatttgatat ctttaagcag tcaataagaa atgaagagcc
     5641 ttctgagtgg ataccggatt ttgtatatgg agaggaagta tctgctcggg catacctcaa
     5701 aattatggca aaaaatactg ttttagattt tgttgcaaga actatgtcaa cgttagaagt
     5761 taagtttaaa aataaagatg ggacatcaga ctgggattat attctaaatg ttcgacctaa
     5821 ttctgatatg tcagcagcta cattttggca aaaatttttt tatcggctct tagatgacaa
     5881 cgaagtgtta gtaatattta cagatgataa tcaattgctg attgctgatg attttagtcg
     5941 aacggagtat gccgtgtatg atgatgtttt tacaaatgtg atggtaaaaa attttgtctt
     6001 tgaacgttca tggaacatgt ctgacgtcat ttatatggaa tataataacg aagaattaga
     6061 aaggtttaca cgaggtttat ttgaagatta cgccgcgttg ttcggacgga ttctagaagt
     6121 tgcaatgcgt aacaaccaaa ttcgtgggtc tgtatccatt gattcaacgg gaacggcgaa
     6181 tgatgaaaaa ggaaaagatg gtaagactag atctcaaaga ttacaagaat atattgatag
     6241 gatttggaac tcttttaaaa ctaactcagt tgcaattgtt cccaaagtga aaggctttga
     6301 gtatgaggaa tacaccaata aacaaggcgt atcgaatcaa tcgctagatg aactgaataa
     6361 gatgaagtcg tctttaattg atgatgttgc caacgccata ggagtaccta cggcgcttat
     6421 ttatggtgaa aaagctgaac tggattcaaa tattaaagct tttcgtaaat tgtgtattgc
     6481 tccactagtt aaaaaacttc aagacgaatt ggtggcaaaa ataattagtc gtcaagagta
     6541 caaaaacggg gaacgtatag aagttaaaaa agttctccaa aaagatctat tagagtatgc
     6601 tactcaaatt gataaaataa catctggtgg tgcgttttat attgacgaag ttcgtgatga
     6661 agttgattat gatgagttgc caaataatga aggaaaaatt cgtattcgta cgaaaaacta
     6721 tgaagaaact gtgaagggag gtgagaacga caatgccgaa agctaaaaaa gtaccatttc
     6781 agtttactaa cgaaattcag aatggtaagc acattctcac cttgagtgga aatgtacaga
     6841 aaaaatattg gcgtgatgat gatgtcataa acgctaaaga tattcgtgac tctctagata
     6901 ctgtaaccga tgatattgta atcaagttga acagcccagg cggtgatgtg tttgaaggga
     6961 ttgagattta caattattta aaagatcacc catcaaatat tactgtagaa gttacagggc
     7021 ttgccgcaag cgcagcaacg tttattgtcg ctggtgccaa tgatgtgatt atgaatgttg
     7081 ggacttcttt gatgattcat gaagcttcta catttgcttg ggggaataaa caggacatcc
     7141 aaaaaacgtt aaacgcattg gaaacgatcg atgattctat cttagctatt tattcagata
     7201 agactggcca atctgttgat aagttgcgtg aatggatgaa tgaagaaaaa tggtttacag
     7261 cagatgaagc tgtcaaatat ggatttgcca attctgtgaa gcgtgaagaa ccacaaaatg
     7321 aacctcaaaa tattgcagcg atgattcaag atgctgttgc cgttgcaata gcgaatttaa
     7381 aggaacctgt tactaataaa atcgaacaag aatcaaaaca aaaatcatta atcgcacgat
     7441 tgcgaaaagg agaataaaga tatgttaaaa attacagata aaactgcaga tgcaaaaaag
     7501 gcatttaatg ctatttcttc gaaagaagat gcaacacccg aacaaattaa tgctgctcta
     7561 gaagaatatg taaccgccat tgcagaagat gccggcaaac aggtgcgtgc tgaatatgaa
     7621 gaattgaaaa atgtgacgga caaccgtgta ttagaggctc gtggaattcc tacactaaca
     7681 gcagaagaaa caaagtttta taatgaagcc gtaaaatctg gaggtttcga ttctgacgta
     7741 gtttggccag aaacgatttt ggaacgtgtt tttgaaaatc ttcaaaacga tcatcctatt
     7801 ttaggcctca ttaactttac accaactgta ggtcgtgtca aagtaattag agcacgtcgt
     7861 aaaggtgttg ctgtttttgg accattgcat aaagaccttg aaggccaact agatgctgaa
     7921 tttggtgcaa ctgaatttgt tcaacttgcg ttgaccgcat ttttcttaat ttctaatgat
     7981 accttagatt taggaccacg atggattgac cgttttatca atctttcttt aagtgaagca
     8041 gtacgtgatg tttgggctaa aaaaattatt acaggttctg gtaaagatga gccaattgga
     8101 ttgcttaaag atctagatgg tgcggtcaca agtggtgtat atccagataa agctagcgca
     8161 gggacattaa cttttgcgaa agagaagatt gttcttgaac ttgcaggggt gatgaaaaaa
     8221 ttatctaaat acacatacaa agtagatggt gacgatgaag gtgaagtgaa ataccgttct
     8281 gttgctggac gagtgtatct aattgttaat cctgttgatt actgggatat cgtagctaga
     8341 gttacatttg caaatttaaa caatgtgtat gggactacga tgccatttat cgctgttgat
     8401 catatcatcg aatctgtaga cgtaccagaa agtaaatgta ttgcatacgt tgataaagag
     8461 tatgaagcta ctcaatcaag agctgaaaaa gtctatgtat ataaagaaac ttttgcaatg
     8521 aaacgcgcaa ctttgtatgc tgtcgacatg cttggtaatg gctatccaac aaataatgat
     8581 gcagcacaag tatatgactt ggactttaca gcgggggagt aatgtcccct gcaattggtg
     8641 agataacacc gacgactgat ggggcagtca ttaatctttc gtaaatgggg ttagagtatg
     8701 aacactgaag aatttataaa agaatataag gcccgatttc gtattttcca tacttccgaa
     8761 gatgaggata tcgggaaaca gttagagagc gggtttgccg atataaaatc tctaatcggc
     8821 agttttgatc cttccactta tgagaaagga aaagaattag tttatgaacg tactcggtat
     8881 ctacggaacg aatcactaga gtacttttac gacaattttc aaacaatgat tatggatgca
     8941 tcaattgatc tggcaggtgc tggatatgcc aattaaatca aattataaga agcctaaaat
     9001 tgtagctggt gatctaaata cgccagttac tttttttgag tttcaaccta ataagggtcc
     9061 agaacctggt gatgaagaaa aaaaggaact gtatttttgt acagcactag tttacaaccc
     9121 atcaatgaaa gatcgtgaca ttcttggcac aaaagaaggc gttacgataa aaatacggga
     9181 tccccatcaa tcttatattc ctactaacaa acataaagta aggatattag attatcgata
     9241 tggagataaa gtttgggaaa taatcgatgt ctcaccggat ttggaagaaa attcaattgt
     9301 aaagatagta ttagaattaa ttacttaaat ttggtgataa atgatgagta acgtaaagtt
     9361 tgaaggcgtt caagaaacaa tccaagcttt agaaaaaaag tatggagaaa agaaaactaa
     9421 gacattaaca aaaaaagcaa tcaatgttgg cgctgaaaaa gtagaaaaac agttgcaaac
     9481 ggacatgacc gtttttaaag acaaaggtta tacgatcgct gaggttgttc gcaagaacgc
     9541 tacgtataga gattacaagg cagaagctga aatcggttgg aatggtccac atcaacgata
     9601 tagaatcatt catttgaatg aatggggata tacacgaaag ggcaaacaaa ttagaccgag
     9661 aggatttggt gtaataacaa aatctcttaa gaattccgag ctgttgtact ttgatgctgt
     9721 aggagaggag gttaaaaaga gtttatgaaa gatatgctta atatcattta cgaggctctt
     9781 tgtttgaatg agtatattca ttcaatgacc tacaacgaga ataccgagca atatcgtatt
     9841 aaattttatg agcaacctga aaccgccgat aaaacaggtc caatgatcac aattcgtcct
     9901 gtagatacac ctaacgaagc gtaccacgga agtaacaaag agctttctgt ggagcatatg
     9961 atacagattg acgttgaatc gaaatataga gcaacatgca agcagattca atatgagata
    10021 aaaaaagaga tgaaaaaact aggctttggc caaatttccg gtcaagggtt agatgaatac
    10081 tttactgaaa caaaccgctt tgtagacgcg cgtcgttacg aaggaaatac aaaaatttac
    10141 gataccgaat attagaaatt aagacacgaa aataacgtgt ctttttttat tacccaaaat
    10201 taggaggaaa attttatgac tttagtagga ttcaaaaaaa tgacaatcgg ggtctttgac
    10261 agcacaggga agattccagc agcaaattta tatgtaattg aaggtgaaca aaacaaaggg
    10321 gcgactgtat ctgctgaaat cagtgggcta tcaaaagaac catcaaaagt ttacggatcg
    10381 aacatcgctt actatgtctc tcaaaaagga acaggcgatg tttcagctac ttttggattg
    10441 ttagatttac caacagaagt aaatgacaaa attcttggtt acaaaaccga tacaaacaaa
    10501 atcagcttct tgggagaaga tactgagcca ccatattgtg caattttgat ggaatcggag
    10561 gatttgaacg gcgatacagc aatgcttgcg atgttcaaag gaaaattcag tcgtgaatct
    10621 attaatctta acactacgac aaatgaagct ttcgaaccag aagcagaaga atatgtattt
    10681 tcagcgattg ccaatgatgt tgaaggtgat gctaaaggcc aaacagtcgc aaaatatgtt
    10741 ggagacgaag aagcttctat tactggtttg aaagagatgg tatttcctgc agcgggggag
    10801 taactagccc cgttgttgga gcaatcactc ccactattaa tggggcaaca gttgaattaa
    10861 gttaggagga taactatgcc agatacattt aaaatctata aaaaaaatgg tactgaattt
    10921 acaaaagtcg cagagggtga aagtccactt tctattactg gaattgcagc aaatactcaa
    10981 gttgctaaag gagattatca agctacgcgt gtggtcggag gtcaagaatc agtaaaagta
    11041 gatattcctg cattcaccac tcttccaatt tcagttactg gtgtgacgtt ggataaaacg
    11101 acagctgaag ttgaacaagg cggaacctta aaattaactc ctactgtaac accagcaaat
    11161 gcaacagaca aaacaggaag ctggagcagt tcgaatacag ctgtagctac ggttactgga
    11221 ggcaacgtta cagtaaaaac agacgctgaa gttggcggaa ctacagaagt ttcatttact
    11281 actacggatg gcggtaaagt agcaaaatgt actattacag ttattgaaaa agcagcgggc
    11341 tagtcttcgg actagctttt ttatttggaa aataatggag gaatctaaat ggcaaaagta
    11401 agaattgaat tgaaagaaga atcaggaaag aaagtctatg aaaacataga cacaacaggg
    11461 aaagattatc gaaaagcttt agaaacaatc aaaaagctaa atgaagacgg agtaatgatt
    11521 tggaatcgtt tagacattta tttggatttt gctgtaggta tcttcaggga tagcaaattg
    11581 acagctgaac aaattcttga tggcttacca tctgagaagg taatgagcac attggacgac
    11641 atcctaggag aagtaatggg gattgaaagt aatccagacc cagacgcaaa aaagtaacac
    11701 ccgaagaagc agaagaaatg tacttaaacc tatgcagaga gttagtgaaa gcgggatggt
    11761 cactatctga catagaagat aattcctttg aaatgatgat gaaaattgcg tgcacttcgc
    11821 ctaagaaaga gaaacaaaaa gaagttgatc ttaaagactt cttgaaatcc atgtagagag
    11881 gaggtaaaca tatggcaaat ggaaaacctt taggcaatat gattatcaaa ctagacttag
    11941 acagttcagc tttttcaaaa ggtctagccg gagctaaaaa cgctgttaac catcaaatga
    12001 aagcaatgaa atcacagatg caagtaatga actcttcagg aaattctctc ggtgctttac
    12061 aagcaaaata caatggttta ggcggagttc tacaagcaaa tgaaaagcaa gtagaattgc
    12121 tcactaaggc ttataaggat agttttgact ctaacggaaa cgctactgct tcgactgcta
    12181 aatacgctaa tcaattgaat caagcaacag ctagatcagc aagttacgaa tctcagatga
    12241 aaacaactgt tggacagatt gctcggatga aagtggaaac ggaagggttt acggggaaac
    12301 taaaggctaa ttctgatgta ttgattaatt ctgggaaaaa aattgaagcc tttggtaaag
    12361 gagtttctgg gataggatct gccctaactg ttggagtaac agctccaata gtggcaggcg
    12421 caactgctgt tactagtgca gctatttcat gggaaagtgc ttttgcaggt gtaaaaaaga
    12481 caaatgatga agttgtagac tcgaatggaa acgtagttta ttcctatagc gacttagaga
    12541 aaggtctgcg taatcttgcc acccaattac ctggtagcca tcaagaaata gcaaatgtag
    12601 ccgaagcagc cgggcaatta ggtataaaga ctgaaaacgt agttagcttc actaaaacga
    12661 tgatagacat gggtgaatcg actaatatgt cggctgaaac tgcggcaact tctttagcac
    12721 gatttgctaa tattacccaa atgtctcaaa aagactttga taaattagga tcagtcattg
    12781 ttgatctagg taacaatttt gctacaaccg aatcagaaat cactgaaatg ggattgcggt
    12841 tagcgggagc tggtaaacag attggaatga gccaaggcga aattctagga tttgctactg
    12901 ctctatcttc tgttggtgtc gaagctgaag cgggtgggtc tgccttttcg aaggtcatga
    12961 tccagatgca actagctgta gaaaaaggat ttggcgcttt tgatcagctg aaacagatgg
    13021 cagaagaaca aggtgttccg tgggttaatt tagtaaatgc tgtgagagac ggtggaaaat
    13081 cgttaaaagc agtatcagag caaatgggat tcacttcatc tgatttaaag aaaatgtata
    13141 aagaagcgga caattcgaaa agttcgcttg aaaattttgc gaatgtggca ggtgttacaa
    13201 gtgaacaatt ccagaagttg ttcaagagtg atccttcaga agcaatcatc aagttcattc
    13261 aagggctgaa agattcagaa aaacatggta cttcagcaat caaagttctg gatgacatgg
    13321 atataaaaga agttcgacta cgcgacagtt tattgagagc tgcaaatgct agtgacatat
    13381 tcaaaggagc aattgaaaaa ggaaacggcg cgtggaaaca aaatacagct ttaacaaatg
    13441 aagctaataa aagatacgaa acaactgaat caaagctaaa gatattgaaa aacgaagtga
    13501 ttgatacagc aatcgatctt ggaggtccat ttgtagacgc gttgcgtgat ggtctaaatg
    13561 tatcgaaacc attgataaaa aacttaggga atatggcgaa agctttctca gctatgagtg
    13621 atgatcaaca aagaaacatt atcaaatggg ttggattagc agcagcagct ggtccagcgc
    13681 tgaagctttt aggaactggt acttcagtaa taggtaaagt tacttcaact actggaagat
    13741 tgacgaaaag ccttgttgat ttagcagcga aagcagcaga aaagaaggct atggatgcct
    13801 ttgcatcgtc ggtaacaaca gctggtgctg cagctgcaaa gacagcagga gctggaggta
    13861 ttggcgggtt agtccctatg atgggaaaaa cagctagtgc tgcaagtact gctgcgggcg
    13921 ccagcggaat aggcgcaatg acaacgtcac taggtttgtt aggtcctgct ctacttggta
    13981 ttgttggcgt tggtggcgca ctggcagttg gctatggagc atggaaatta tttggtgaag
    14041 aagcttggaa ttcctcgcaa agagtaaagc aatggggaac agatgtcggt gaggagatag
    14101 atagtactct tgacggagta cagaagaata ttgaaggtgc aaatggtcaa ttcggtcttc
    14161 tcaaagaggg ttttactgtt gatgatgcaa ataatatggc ggctaatttc gcaaccgctg
    14221 gaacatcttt ggaaacatct ttaacgggta gaataaaggc attagacgat gcactaaaag
    14281 ggctacctca atcagttcag gatgccatga aagaagtagc tgaatcagaa gaagaaacct
    14341 tagggcaatc tctttctaaa ataaaagaca acaatgaaga aataaaaaaa atcagagaaa
    14401 gagccgcaaa tcagaacaga gacttgactg tttcagaact acaacagata caggatctaa
    14461 tgaaaggaac ttctcaagcg tatgtggata cgctcgatgt ttctgctaaa gacagaaaaa
    14521 agatactatc agccatgaat ggtgatgttg cacaagctac agaagaagaa gcgaagacgt
    14581 ggttacaatc tttagcgaaa caaaaagctg caactaccga aaattataga caaatgcttg
    14641 aagaaaaaaa ggcggcttta aaggaaaagg gagtaagcaa agaattcatt gatgcattag
    14701 aagatgaaat gaatgattac atcaaaacta caaatcaagg attcgatagc caaatagctg
    14761 ctataacaaa taaatatcca caattgatta acgaagtgag tttatcaaat ggtcaattat
    14821 ttagcaaaac ggcagctgct atggacaata ctggtcagta tacaaaagtt tttctcgaaa
    14881 gtaatgaaaa gattttagcc caagcgaaga aaacatcgga tgaaatagca gataatgccg
    14941 aaaaaaatgc agaacaactt ggtatggtag ctgatgaggc tacgcatgct ggatcaactt
    15001 ggaatggctt agttttagac gaaaaaacag gaaaagtaaa aactaatgta caagaagtag
    15061 tgaatgaagc aacgaaggat ttcactaaat ggaatgaatt gaaacctgtt gttcatgatg
    15121 cgaaactaac aacgaatgca aaagatgtca tttccatagc ggcagtacaa aatggctatt
    15181 gggatgatat ggaatggaaa gaaaaagaag cattattaga agatgagtgt tctaaaaatg
    15241 tatacaaggc tttagaaaat gctggtaaat ggaatgagtt agatattcct tctaaagaag
    15301 caatcctaac ttcaaatact cctgaagtta tgggtgaaac tctcatgaat ttgggattat
    15361 gggatactta caaacttcaa gtgaaagatt tagatttgaa tgcgtataaa gtatacgatg
    15421 ctatcgatgg atcagaagaa aaattaaata gttgggctca attagataac cccacaaaag
    15481 aattgttatt agataactca gatttttcta caaaactttt taattccgaa gaaatgttta
    15541 aacgttggaa tcagttatca tctaatgcaa agtttctttc ggctaataat actaaattta
    15601 cagaggttct tttatcatct gacagaatgt ggaatcaatg ggtaaacctt cctgataatg
    15661 agaaaagatt acttgcagat aacgaagatt tagctaccac tgttttatct tcagaagaaa
    15721 tatacggaag atggactcaa ttaccagata atcaaaaata tatgttagct gacaatactg
    15781 acttagcaaa caaaatattc gcttcagaag aatcatggaa tgcatggaaa gcaattccag
    15841 acacagttaa aaacttgctt ggtaataata ttgatttact ggctaaagtg aaagacggga
    15901 ctgtaagtgt agaagaatat aacaaagtta tgccacttct taaaactcta tatggagata
    15961 acgttaattt aaaagaggca atcagtgggg ctaaaagctc tatagagaat tacaacaaaa
    16021 atatttttcc tagtgataaa actgcagtag gacacgatga ggcttcacaa gctgctaaag
    16081 atgcatttga agcgttcaac atcttccaaa caaaaatacc agataaaatt actaaaactg
    16141 tatcagctga ctttataggt cctatgccga aaaatgcgaa aggaacaaac ttccatcctg
    16201 gcggagcagc tatggtaaac gatcaaaaag ggcctactta tgaagagctt ataaccttgc
    16261 caagcggaga aacttttata cctaaaggac gtaatgtcgt tttagactta ccaagaggtt
    16321 cgaaagtact gaacgccact aaaactaaac gtctagtgcc taaatatgct gatggtatag
    16381 gaaacataac gacggtttca tctacgctaa acttagatgc tttaatttta gctattaacg
    16441 agctaacgac ggtattgaga actcaactga tggcaggaac gactgatgct aaagatacta
    16501 aactatcaga tgaaacaaaa gttgccgatc cgattattcc ggacagtctt tctgaaaaat
    16561 cagatcagta tctaggaatt ggagctcaat ggttaactaa tctaatgaat ggttggaact
    16621 cagcggttcc tcaatatatg aatagtgaaa tggtttttat tacaaactat ctcaatgcgc
    16681 taaaacttca aaacaatcct aattatacgc aaggagcaac atggaataag aacttactga
    16741 atggatggaa tagtttaact gggaccttta ttgctacaat caattcattt tgtaatcaag
    16801 caatggtcac gcttagaaac tacaacactc ctatgtacaa caatggccga acgtggcagc
    16861 aaaataatct taatggttgg aattcattgt atggatcatt catagcccgt gtaaatcagc
    16921 ttggtaacga ttcgattaac aaccttcgtt ctaaaagtgg tggtttttac aatgcaggga
    16981 cattcttgtt gcaatcttta ataaacggaa tgaattcgtt aggtaattct ctttctacta
    17041 caatgaacaa tgtcgctaat acaatggttg gtggtatagg caaaggagtt aatggcgtca
    17101 tatcgggagt taactatgtg cttaaggaag tcgaatcaag taagaatatc ggaaactggc
    17161 caattccaca atacgctaag ggaactgatg gacatcctgg tggacttgct atgattaatg
    17221 atcaaaaagg cctagttcac gaagaatatg ttcagatgcc agatggacgt agttttattg
    17281 ccaaaggtag agatcttctt gttaatttgc ctaaaggtgc tcaggtctta aatgcttctt
    17341 taacaaagaa attaaaggaa cgtttaaata ttccacacta cgaaaaaggt attggcaatt
    17401 tagatattgt tgatttgtta gatgatgaaa aaagaatgtt agaattctta actagcaaag
    17461 ttgatttttc aggcattaat gaaccatggc ttgatatgac taaatcagga acgtcattaa
    17521 tgtccaaagc tgcaaataca atgcttcaat caaaattaag tgaattcttc actcatggca
    17581 attttgatgg tgcagtaaat gccaatggtg tttatcaata tttagttgat gtggcacaga
    17641 aagtaatggg taagttcccc ggacttacag taacttctgg atatcgagca ggagatgcct
    17701 actatcacgg aaaacggcaa gctatcgact tggcttatcc aggtatttct ggagatccga
    17761 gatatacagc agcagcaaat tacgcttttg aaaaattccc ttcaaaaatt gcgtatgtca
    17821 ttacgaatgg acgtgtacgt gaccgcatgg gattatctgg aacaaggtca agcggacaat
    17881 ggacgaattg gccagatggc gatcacttcg atcatattca tttaaacggt tcgatggggt
    17941 ctggcgatat attcaatgga ggagttggag gaagtggtgt tgcacgctgg cgttcttatg
    18001 tgtctaaagc attgaaaatg aatggattac cagctacagc agcgtatata aatgcctgga
    18061 tgtcccaaat ccaaacggaa tctggtggta atgagaaagc aatcggcggc aatgatgggc
    18121 tcgccgaagg caatgcaaca ggattacttc aaacgaaacc cggaactttt gcagctaacg
    18181 cttttcctgg tcacggaaat attatgaacg gatttgataa catgttagca gcaatcaatt
    18241 atgctaagaa acgttacgga gttgcaggta tgttgcaagt tatcgggaaa ggacatggat
    18301 atgccaatgg cggcttgatt accaaagacg gactatatcg agcaggagaa ggaaataaac
    18361 cagaaatggt tattccattg acaagaaaaa caagagctat cgaacttatg ggtcaagcat
    18421 tagccttcct ttctggaagc aataaaaaag aaacgaaaag tgagattaat tctgtagata
    18481 atagacatat cctacaagcg atagaaaaac agtctcgtat gacaaatgat ctgctatcat
    18541 tattactctc gttttttaaa agtaacaatc gatcagataa agaactagct ttagatattc
    18601 agaaaatttt aatcaggagg acatagtatt tgaggacagt aatattaaga aataaattga
    18661 atgaagaaat tgatttgtcc acagaagatt attttgctac tgaaatgtcg aatctaggtt
    18721 ttgaagttac aaaagagcat attggacaat gggggagttt cagagaaata ggagagagta
    18781 ttgaattgtc tgaatttcaa tcatcagtta tgatttctgt tcacggattt agagaacaac
    18841 aattatatgg ttcattagtt gaatttctgg ctaatggacc ttttgaacta gaattttctt
    18901 ttgattcaga aacaatgatc aggaaatgta gtttgaaatc attatctaaa acagaaattg
    18961 attcccaaag ctcattgcta acagatactt tgaatctata ttttacttca agctggtatt
    19021 ctattaagcg tgaaaaatta atacagaatt caacttctct caatacacgt ggaaaagttt
    19081 actcatttgt tcgaccatat gtctacacca aaaatgtatg ggaaaagaaa ggtgtattta
    19141 agttcagtaa taattctgta tatttgacaa atagtacaga gcgaatgtct ccgttaaaaa
    19201 taaggattat tggagagtgc tcaaatccat attgggaagt tatccaaaac tctaaagtag
    19261 tagcttcaga tggctatttt attaatatga aagacacaca aatattagaa gtgtctagtt
    19321 tgctagaaga ccaaacggca attttaaaag atgtaggtgg agttgaatct tctgtttatc
    19381 aacaacaaga ttttactaaa tcaaacttcg tccaagcgcc tctaggagaa ttcagcgttg
    19441 tttttcatgt tggacatgca aatgtagaga ttgaacttta tgaggagcgt gatctatttt
    19501 gattttagcc attaccctct ttcaccgaga tatgaattta tataacgaac gtttaatttc
    19561 gactgagttc gaatttggaa cggatgaaat tggagaagaa gtaagtaatt ttattacaga
    19621 taaatatgtc catgtaaaat caggagattt tctactagca aaatttattc ctagcgggaa
    19681 gattgcttat tttggagtga ttagctcgca agaagacaaa aaaataaatt gcaaaggatt
    19741 actcagtttg gccgatagcg agataccgac tgtaaaaata tcaggtgcta attatgaaga
    19801 gcatattcga cgactgattg aatactactt attgaacgat ccaacaaaac aactaaaaaa
    19861 tattttggag gtaaaaacag aaagcgttac ttctcattct tatcaagctg ttgacacaaa
    19921 taagagacga ttgaatgcat atataataaa tgggttcaaa aaatataatg ttaaatggta
    19981 tgtcaaagag attaaaggta aaaaaatata tactggtatt cgagcagttg atgattcaat
    20041 ttatattaaa gataattcat ctgagtttaa tgactgggat gtttttgtgc aatctcctgg
    20101 accagggaac gagaacaaat tattgatcgt agataaagca atgaaagata tagaaaatcc
    20161 cttggtattg tctacgtggt atttagatga cgaaaataat cttacgcaag attcaacgaa
    20221 taagaacatt gcattgccta ccgttaattt agtaaatata tatgatcaaa ctcaagaaga
    20281 tagaccttct tatgaagagg tagctaaatc ggagcttaaa ggcaacgcct attcacatga
    20341 aattaaagtt aatgttgtgc taaatgctag aaatatcaat gtagaaaata tcgaaacagg
    20401 aatgttagcg actattgttt acaaagagca gacttacaaa tctgttctaa ctgcctggcg
    20461 aatatccagc agtaaaaaga atatagaatt gacatttggt aatgtccgaa gtcgttttat
    20521 ggattatttt gaagataatg ggggataaaa aatggttagc aatgtggatg gatatcaatt
    20581 tgaaaacgtg aaagtaagcg cagaaaatga tgctagactt tatcacgttt tatataatcg
    20641 gaaaaatcag gttattgatg gttacgatca gtctatgaat ttatcttcaa gcggattaac
    20701 agtaaaagtt gctgcaggag cagcgattat tcaaggtcgt atggtcgttg ttcgacaaga
    20761 agaaagtata acagttccag caaactcaag tggttatata gcattaacag tggatttgac
    20821 acaagaagtt atacctggat ctattcttcc agaatcggaa gaatatgaat ggactaataa
    20881 tcaagtcaag ctagaattta taacaaaagt tataaaaggt aatttaaaca atggtgataa
    20941 ggtctataat ttaccactat gctcagttaa ttctactgga tcaactgttt caatctcaaa
    21001 gatatcggat agttacgagc tgactctctc taaaggagaa attttgtgga gggggactgc
    21061 gttaatgcat gatactcaaa ctgtccaacc ttcaaaaaaa atttggcaga cagtttcagg
    21121 ttttttgtta gtatggctcc catacgaaaa cgggcaagca attgaggata gatatgtaac
    21181 tacgcctttt tataaggagc gtataacttt tactaatgtt ttaggagaaa ttgtttcagg
    21241 atttgatgac tatcacaaaa agtggtttag taaacgaata aactataatt cgaatactaa
    21301 tatatttaca ggtgcagcaa gcaatgcaag tggagataac gcaaatatgg tgcttaggta
    21361 tatagtttct ttttagttag gaggtatgga agagtggcaa atttagaaat taaattatct
    21421 gcaaataaga gacagcctta tctacgtcac cgtgttgttg gtagagttgg tgatgggggg
    21481 ctcacaacaa tcaatgtaca acttcttgag gaagatgaaa ttacaccttt tgtaattaat
    21541 ctaaacggta ctttgaaatt tgtgggcgaa gtttcaaacg gtaactatac cgagggagaa
    21601 ccagaaataa tcgattcgac taatgggtta attagttaca cgttcactaa gtcaaatttc
    21661 agcacgagtc atcaattcaa acaagcatat tttgaatatg tagatcctaa cggcaaaaaa
    21721 gtaacttttc agaacttcat catagacgtg ttagaacgag tagatattaa ttcggagcaa
    21781 gcgaaatact atatttcttc attggaaaaa ttacagagtg aaatgcaaac cactttcaat
    21841 cagttcatta gtgataaaca ggtccaatac gatcaaatct actcgaaata taacgaatta
    21901 gtaagattga taaatgaatc agataagcaa gtaaatgatc gtattgataa ggcaaataat
    21961 cgtattgatc aaaccaatca gcaaatcggc gatctcggca agctgaaaaa gatgtacagt
    22021 aacagcattg a
//
LOCUS       DAYYCY010000029        21912 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000029 DAYYCY010000000
VERSION     DAYYCY010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21912
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.029"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="ACHAZB_000909"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="ACHAZB_000909"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFM8613600.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="ACHAZB_000910"
     CDS             complement(1172..1867)
                     /locus_tag="ACHAZB_000910"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HFM8613601.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="ACHAZB_000911"
     CDS             complement(1880..2596)
                     /locus_tag="ACHAZB_000911"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HFM8613602.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2926..3534
                     /locus_tag="ACHAZB_000912"
     CDS             2926..3534
                     /locus_tag="ACHAZB_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HFM8613603.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3859..4182
                     /locus_tag="ACHAZB_000913"
     CDS             3859..4182
                     /locus_tag="ACHAZB_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HFM8613604.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4364..5734
                     /locus_tag="ACHAZB_000914"
     CDS             4364..5734
                     /locus_tag="ACHAZB_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFM8613605.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5864..7165)
                     /locus_tag="ACHAZB_000915"
     CDS             complement(5864..7165)
                     /locus_tag="ACHAZB_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFM8613606.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7519..9510)
                     /locus_tag="ACHAZB_000916"
     CDS             complement(7519..9510)
                     /locus_tag="ACHAZB_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HFM8613607.1"
                     /translation="MATTHNTKKISMTGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9654..9920)
                     /locus_tag="ACHAZB_000917"
     CDS             complement(9654..9920)
                     /locus_tag="ACHAZB_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFM8613608.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="ACHAZB_000918"
     CDS             complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="ACHAZB_000918"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFM8613609.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="ACHAZB_000919"
     CDS             complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="ACHAZB_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFM8613610.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13264..13827
                     /gene="def"
                     /locus_tag="ACHAZB_000920"
     CDS             13264..13827
                     /gene="def"
                     /locus_tag="ACHAZB_000920"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFM8613611.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14080..14601)
                     /locus_tag="ACHAZB_000921"
     CDS             complement(14080..14601)
                     /locus_tag="ACHAZB_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HFM8613612.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14604..15446)
                     /locus_tag="ACHAZB_000922"
     CDS             complement(14604..15446)
                     /locus_tag="ACHAZB_000922"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HFM8613613.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15439..16164)
                     /locus_tag="ACHAZB_000923"
     CDS             complement(15439..16164)
                     /locus_tag="ACHAZB_000923"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein lysine
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HFM8613614.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16340..16636
                     /locus_tag="ACHAZB_000924"
     CDS             16340..16636
                     /locus_tag="ACHAZB_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8613615.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     regulatory      16892..17066
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            17204..18796
                     /locus_tag="ACHAZB_000925"
     CDS             17204..18796
                     /locus_tag="ACHAZB_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFM8613616.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18809..19549
                     /locus_tag="ACHAZB_000926"
     CDS             18809..19549
                     /locus_tag="ACHAZB_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM8613617.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19717..21066
                     /locus_tag="ACHAZB_000927"
     CDS             19717..21066
                     /locus_tag="ACHAZB_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8613618.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21194..21784)
                     /locus_tag="ACHAZB_000928"
     CDS             complement(21194..21784)
                     /locus_tag="ACHAZB_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFM8613619.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVIVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
BASE COUNT         6295 a         4462 c         4158 g         6997 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcaaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttaaaat aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 gaaagcacag gtgaaggtgg cgaaccagaa gtgatcggtt caacagaaga ataatactga
     3541 aacaatcagg tggtgttaga agcggcgggt cagcagcttt taacacgcct tttttatttc
     3601 taatcgttta ttttcatatg gggcttccac ttttgtaaat ggctttacaa acgaaaaatc
     3661 ctaatttacc aagcttttcc tcgcatactt ttatcgtacc tttataaaaa tttatacttt
     3721 gaaaaaaagt attcaattat tgttgcattt ttcctgattc tttattagaa tgaaccctcg
     3781 ttgaagtaag tatttctagt ttatcctagg attaattata aaaataaaac attcgttgat
     3841 tcgaaggagg ttaaacagat gtttgaaagt tttgatagca gccgcaaccg ttacgcttca
     3901 gttggtgtcg tatcaagttt gccagatgaa ttgattgaca gtatatggtt tattattgac
     3961 ctagatctta agggagtcat tccattagaa aatatattaa cctttgattt gatcgaaaat
     4021 cgtggaaaag tgacgatgca tttttctcaa gcaggaagca atgtggaaat ggatattgat
     4081 ttgccgtttg cctattcgaa ccaatttcct gctcaagtat acgcctatga tgacggtacg
     4141 agagaaacga tccttttacc aagtgagatt agacaatatt aaggtgatag ataaacgtgg
     4201 atgtccctta ttttacagac caggccaagt tgttgattct ttgactcaat tcttggcttc
     4261 tttttatgga gtttcataaa ttatcattga tttctaattg gtattctcag gataaacgct
     4321 atatactact aactaaattt taaagatata aggagtttaa aagatgcgcg atttaacaaa
     4381 aggaagcccc gctaaactga ttttgatgtt cactgttcct ttactgatcg gaaatgtttt
     4441 tcagcagttc tacaatatga tcgatatgat catcgtgggt cagacgctag gtaaaaatgc
     4501 gctggcagcg gttggtgcaa ctggaagcct gacatttttg atcattggct ttgcccaagg
     4561 tttaacagct ggtttagcca ttattaccgc acaacgttac ggtgcaaaag attatcgggg
     4621 tctgaagaaa agttttgctg ctagtgttgt cattagtctt gtcgtcactg tgattttaac
     4681 tgtcctaagt ctcctgttta ttcgtccgat gcttcaattg atgcaaactc ctcctgaaat
     4741 acttgatcaa gcacaaacat ttatttcaat tattttatta gggatctttg catcagtcag
     4801 tttcaatttg ctttctaatg tcattcgcgc acttggtgac agccgcacac cactattctt
     4861 tctgatcatt gcggtcatca tcaacgtcgt acttgatttg atctttatta tctattttgg
     4921 catgggtgtg gaaggtgcag ctattgctac agtaatcgca caagtttctt ccagcgtact
     4981 gtgtctagtg tatatcaaga aaaaaatgcc tttgttacag ttacgcaaaa aagatttcaa
     5041 atttgaaaaa gaagagtttg ctgtacacct aaatgctgct ttgccaatgg cgtttcaatc
     5101 ttcgatcatc gctgttggtg ctatcgtgtt acaagcagca ctaaatagtc tagggacaga
     5161 tgtcgtagct gctcaagccg ctgctggtcg tatcgaccaa tttgcaaatc agccgatgat
     5221 gtcatttggt attgcaatgg caactttcac tgcacaaaac tatggtgcga aagaatatgg
     5281 ccgaatcttg caaggggtac gtcaaacttt actgatgagt gtcggattta gtctcttagc
     5341 aggtgctatc gtcattttct tcggtcattt ctttgttcaa ttgtttgtta gcccatcaga
     5401 aacacgtgta tttgaattag cacaaaccta cttcaatatc aatgggtctc tttactggat
     5461 cttagcagtc ctgttcattt tacgctacac acttcaaggg cttggacaaa gcaagatccc
     5521 tacgatcgcc ggtatgatgg aattgcttat gcgttctttt gcagctatcg ttcttacagg
     5581 aatgtgggga tacccaggag cagcagcagc ttctccttta gcttggatcg gttctgttgc
     5641 cgtgttgctt tactcttata tgcgttcgat gaaacaattg aaacacatgg gtgaagagga
     5701 acatttcaca ttagaacatg cagaagcaag atagccttga ttaaaagaaa tcgttgcggc
     5761 taaaaatgaa aacttttatt gaaaatatct tcttcgttta ccgaaaacag gttttttaga
     5821 taacaaaaag gctgtgacaa aaaatgtcac agcctttttt cttttattct gtttttcttt
     5881 ccgtggactc tactttgaaa taatcgatat gtgcatgctg gtattctaat accgtgaaac
     5941 ggaatgatgc aatctcaatg attgttcctg cttttatttc tttatatcgg ctagaagcaa
     6001 agcccgcaag tgtagtaaaa ttcgtttctt caaactctgg aatctctaca tgaaaataac
     6061 gctggaaatc atctaatgtt agtttgccgg agatgatata agagccatct gactgttccg
     6121 ctatcaatgc ttcgtccaaa tctgtctcat ctcggatttc cccgacgatt tcttctaaaa
     6181 tatcttcaat cgtcactaag ccgcttgtcc ctccgtattc atcttttaag atagcaatat
     6241 gcttaccctt ttttcgcatg atcgtcaata atttttttat agggatcgtt tcagttgtta
     6301 cgatcggctc ttcaatcaac tgttctactg tcagctggtc atctttataa gattgcttga
     6361 tcaaatctgg caaggtgaca taacctaaaa cattgtcttt tgattctaag ataaccggat
     6421 agcgagtatg tccttgttca atcgaagctt ttatcgcttc tttgacggtt tctgaagctt
     6481 cgaatacttg catggacgta cggtctactt gtatatcttt tgccaaggta tcatcaaatt
     6541 caaacacgtt ttccaaataa taatattctt ctttggtcaa ctctcctttt tcataagaat
     6601 tcaatgcgac taatgtcagc tcttcttgag acaatgtctc ttctccttca gatacgagac
     6661 gcacacctaa tagcttccct agccctgcag ctgaatgatt caatacccaa ataaaaggaa
     6721 acattacttt ataaaaataa tgaagtggtt taactacgaa caagacgacc ttctccgtat
     6781 tgacgatact atagctctta ggaagcagtt cgccaataac cacatcgata tacgtaacta
     6841 aaataaatcc cagcgccaca gagattgtac tgctaatagc cgaactaaaa ggtagtttac
     6901 cgatcactgg ttctagtaaa gccgcgacag atccttcacc gacccacccg atgatcaaac
     6961 cggttagcgt aattcctaat tgactagctg ataaatagtt atctaaatgg tgtgtaacat
     7021 gaagagctag cttggcgttc tttacatttt ccttttctag ttgttccaat cttgatcgtc
     7081 tgattttcac taaagcaaat tcacttgcta caaaaaatga agtaattgca atcatcaaaa
     7141 ataaaataat gaaattgata accatattct cccgcctttc tctttttcta ttgtaccctt
     7201 tttctacaaa aagattgaaa ataatgcgta ttcatattta ggaataaaaa aggagatgcg
     7261 gcatttttca gccacatctc cttatcactt caaaatcatt gcgttacttg ttgctaaaca
     7321 gctttttcat gacctctgat aaaaacggta ttcaaaagct tgcatctacg gcattaagcc
     7381 caaacagatg aaaaacatga gaagctgttt ttatctgctc gttcttactg cttgttgcag
     7441 tacagctttt tcatgacctc tgataaacgg tgttcaagta gctgacattc gacattagtt
     7501 caggtcaaag cgctactttc acaaccgctc tttcaaccat gttttccgag tatttccaat
     7561 gataagcgga acagtttcat gttttacttc gctgtactct agcccaaacc atgtttctgg
     7621 cgaggtagcg atttttttga ttgccagttt tgcctgcatg atttgacgtt gccattttcc
     7681 tagagttgtc acattggaaa ctacttcatc aatcaggaca aattggaaat cgccaacttc
     7741 tcgccctggt gtcagagagt atctttgtgg ctgttttggc aaacggcctt gtttcatcaa
     7801 atcatggatg acttggcgaa catatacgtt gatttcctgt tggaccctga aacctaagaa
     7861 cagattcact tggacaataa aatcagtgtc catcatatct acttgatatt ctttcgtata
     7921 tggctcatcc gtcacttcca catgaacaaa ccaatagact cgtgctcttt taggccgttt
     7981 atctaaaatc gaatagatga actgtcggcc aactttatct tcagccatat caggaaccaa
     8041 gaagaccaca tttgtctgat agattggtaa agaagtatca tcacgtaatt ctctaagctg
     8101 cgggatataa tctctcaaag acacatcctc ggttgacgat tcgcgaataa tgttcccttg
     8161 ttcccaaata aacatcacga ctaagatgat caaaccaatc agaacggcta caaaaccgcc
     8221 tcggaagaat ttgacaacac ttgagataaa gaaaacgact tctatcgcac cgaagaaaaa
     8281 ggtaactaaa tatgcaagcc aacgagaatg cccattttgg ataagataaa agtaaagcag
     8341 tgctgttgtc atcaacatcg tcacagtgat cgatagaccg taagcagctt ccatatgatg
     8401 agacgttcgg aatgtgacaa ccactagaga acaggcgatc cataaaatca gattgactgc
     8461 tgggatatac atttgcccga tactgttccc aggatacatg atctttaacc gaggcagcag
     8521 tttcaacttg attgcttctg aaactagcgt aaacgaacct gtaatcaatg cttgcgaagc
     8581 aatgactgca gcgactgttg caaaagcaac gccgatcacg atccaacttt gaggcatcat
     8641 ttggaagaat ggattcaggt tcttgatttg ctgtgcttca gggttttgat aaacattcaa
     8701 caaccacgca gcctgcccaa aataattgat aaccaaacaa attttgacgt aaggccagct
     8761 cagacgaata tttcgtttgc cgacatgtcc aagatcggaa taaagcgcct ctgcacctgt
     8821 cgtagccagg aaaatatttc caagaatgaa caagcctaat ttgttatctg cactaaaaag
     8881 cagctggata gcataataag gattcagtgc acggataact gtccagtctt gactgaagtt
     8941 cattaatccc attacgccta aaaaagtaaa ccaagcgaac atgattggtc cgaatgcttt
     9001 tccgacaata tctgtaccaa agcgttggac catgaataaa acaaaaataa taactaaagt
     9061 gataatgacg atcacgttct ggttattacc gaaccggtca aaaaactgcg gaattcctcg
     9121 caagccttca atcgcagtcg ttaccgtcac agcaggggtc aataccccat ctgctagtaa
     9181 ggcagctcct ccgatcattg caggaatgat caaatatttc ccaccttttc ggacaagtgt
     9241 gtagagtgag aaaattccac cttcaccatg gttatctgca ttcaatgcaa tcacgacata
     9301 tttgattgtt gtcagtagag tcaatgtcca aaaaatcaat gagactgatc cgataacaaa
     9361 atcaggattg attcctgaca atccgccatt atcttcaaca atcgatttca taacgtaaag
     9421 cggacttgtc ccgatgtctc cgtagacgac tcccatcgct acaagcaggc cagtcattga
     9481 tatctttttg gtattgtgcg ttgttgccac gtttttccct catttcttgt tatatggata
     9541 tcctttaggt aagcttgaat gatagatgat attcttatcc atgacacccg ctttcctaaa
     9601 ttgcttctaa ttatccaagt aacacactga agtgtcaact tatttgaata aaattattct
     9661 ttctttggac gttctctgat acgtcttgcc acaaatgaat aaagagcaag gcaaaaaatt
     9721 gcacccaata atgctggtat caaataaaaa ccgccgagtt ccggtcccag tggtcctagt
     9781 aaaaattcgc cgagccaact acctaaaaaa ccgataatga tatttccaga tacgcctccc
     9841 gggacttcct ttcctaaaat tgcgccagct atcaaaccta atataccgcc gataaataac
     9901 gaccataaaa aattcagcat ctcctttccc tcctttatcc atatttctca cttttatttt
     9961 acgctttatt cagaaggact ccaaaaatat gcgccaagaa actccagaaa aaaagaagtt
    10021 gtaacaaaag tgttcagctt atttccgtag aagctgcttc tggaacaccg tgaatcagag
    10081 gttgtgaaat cagcggtttg acctgagtat tagagaaaca aacggaaaaa tagttcctca
    10141 tattttgccg ttttttgagc taatatcgag ggacagctga ttgaacatcg tttattggag
    10201 aggttgtaaa aaagctgttc tgcatcaagt aataagaaca gccaaataaa aacagctcct
    10261 aatgttttta acttggctgg acttattacc gcagatgcaa gcttttgaac accgtttatt
    10321 cgaaaaaaaa ggactgtgac acggcttttg tcacagtccc cttttttcga ataattattg
    10381 tccccctgtc accttcacga tcacaagtac attattccta tgaattaaat tatttttctt
    10441 catttttcgt tgaaccatct tcgttcaaca ttgcttttct tgacagattt acgcgtcctt
    10501 gtttatcgat ttcagttact ttcacaagga tctcatcacc tagtttaaca acatcttcga
    10561 ctttattgac acgatcgttt gctaattggg aaatatggac aagtccatct tttcctttga
    10621 tcaagttgac gaatgcaccg aatttttcga tacggacaac tttccctaga taaacttcac
    10681 caactttgac ttctttggtc aaatcttcga tgatcttgat agcttttttg atcatttctg
    10741 catcagctga agcaatactt acttttccat cttgatcgat atcgattttc acaccagttt
    10801 catcgatgat tccgttgatc gtatcgccgc ctttaccgat aacatctttg attttagcag
    10861 gttcgatctg gatcatttcg atttttggtg catattggct taattcttca cgtggagcag
    10921 caatcgttga agtcaattct tctaagattt ccatacgtgc ttgttttgct tgagttaatg
    10981 cttcagttag gatttgttct gtgattcctt gaatcttgat atccatctgt aaagcagtga
    11041 taccatcttt tgtaccggca actttaaagt ccatatcgcc aagatgatct tccagccctt
    11101 ggatatcagt taggatcgta tagttttcac catcagaaac aagtcccatc gcaataccgg
    11161 cgactggcgc tttgattggt acacctgcat ccatcaatgc taatgtgcca gcacagatac
    11221 ttgcttggga agaagaacca tttgattcca atacttctgc tactaaacgg atcgtataag
    11281 gaaattcttc ttctgaaggg attacttgtg ctaacgcacg ttcacctaat gcgccatgtc
    11341 cgatttcccg acgccctgga gagcctgcac gtcctgtaga accaacagaa aattgaggga
    11401 aattgtagtg gtggatgaat cgtttgctga cttccacccc taaaccatca atgatttggt
    11461 gctctcctag aggtgcgagt gtacaagctg ataaagcttg tgtttgtcct cgtgtgaaca
    11521 aacctgatcc atgtacacgt ggcaataagc tgacctctga tgacaatgga cggatttcat
    11581 ccaatttacg cccgtcagga cggatcttat cgatggtgat caattcacgt acgacatctt
    11641 tttcaagatc ttctgtgatt tgtttaactt ctttcaacaa gtgatcttca tcttcatgac
    11701 ctgcaaattt ctcagcataa gcttcgcgga tatcgatctt cacctgctcg atattatctt
    11761 cgcgagcaag tttttcttca gtcattactg ctgctttcat tgtagcgtaa gaagcatcaa
    11821 agatttcttt tttcaatact tcatcaactt gcagtaaagt cacttccatt ttttctttcc
    11881 ctactgcttg aacgatttct tcttggaaag caactaactc tttgatagcg tcaaaaccaa
    11941 acagcaatgc accaagcatg tcttcttctg aaacttcttt cgcgccgctt tcaaccatat
    12001 tgatggcttg tttcgttccg gcaacagtca attcgatatc tgtttgttct gcttgttcta
    12061 ctgttggatt caagacgtac tcgccattta cacgaccaac ttctacgcct gcgattgggc
    12121 cgtcaaatgg gatatctgag attgatagtg ctaaagaaga acctagcatt gctgccatag
    12181 ctggcgagca gtctgtttct acactcatca cgatgttcgt tacttggact tcattacgga
    12241 agccttctgc aaacatcggg cgaattggac ggtcgatcaa acgggcagtc aatgttgctt
    12301 ccgtacttgg acggccttct cgtttgatga aacctccagg gattttccct gctgcataca
    12361 ttttttcttc ataattgatc gttaatggga aaaagtctgt atcttttgct tctttagagg
    12421 ccacagctgc acttaagact actgtatctc cataacggac taaaacggct ccgtttgctt
    12481 gttttgctag ctgaccgact tcgatttgta agggacggcc gccccatgtc gttttgaata
    12541 cttgtttttc tgacataact tctccttttc cttctcgtga aagcaagacg ctactaaaca
    12601 aatgaaaact gccgaaagcc gtcttttgag cagctttcat ttggttagta ggagtccatc
    12661 tagcttgatc acggaatttt acttaattga tatacgagta aaaaaagtga gattcatgtg
    12721 aatcccactc tttttgacta aaattaacga cgcaagccta aacgtttgat caattcgcgg
    12781 taacgttgaa cgtcagtttt acgtaagtaa gctaacaagt tacggcggtg accaactttt
    12841 ttcattaggc cgcggtatga atgatggtct tttttgtgca cgcgtgcatg ttcattcaag
    12901 tggttgattt cttcagttaa tacagcgatt tgtacttctg gagaaccagt atccccttca
    12961 tggcgagcgt attcgttgat gatttcattc ttgcgttctt ttgaaattgc catttctttc
    13021 acctctttcg attattcccc tgcgactgag taaaacgttg gtgattcgct taaccaagtg
    13081 gcaggtcatg tgcctagagc acacagctat actttacctg aaaccgggtt gaaaatcaag
    13141 tagttaatca ttaagaaaag gtagttttca aacaatctta ctactacttt tccatttttg
    13201 tggtaaacta taatgcgaaa atagacttga aacacagtct tttaagaaag gggtacatat
    13261 tccatgatta caatggatga tatcattcgt gaaggaaatc cgaccttacg cgaagttgca
    13321 aaagaagttt ctcttccatt aagcgaagaa gatatctctt taggaaaaga gatgcttgag
    13381 ttcttaaaaa acagccaaga tccaatcaaa gctgaagaac tacacttacg aggcggtgtt
    13441 ggcttagctg ctcctcaatt agatatttct aaaagaatca ttgctgttca cgtaccaagt
    13501 cctgacccag aggcagatgg accttctcta agtactgtta tgtataatcc taaaattttg
    13561 agccattctg ttcaagatgc ttgtttaggg gaaggtgaag gctgtttatc tgttgatcga
    13621 gaagtaccag gatatgttgt tcgccatgcc aaaatcactg tgtcttatta tgatatgaat
    13681 ggtgaaaaac acaagattcg tttgaagaat tatgaatcga tcgtcgttca acatgaaatc
    13741 gaccatatca acggtgtgat gttctatgac cacatcaacg atcaaaatcc atttgcacta
    13801 aaagaaggcg tattggtgat cgagtagaga attggcaggt tccttcgata ctatgctaca
    13861 agaaagcttt ataaaaaagg ggagtgctca aaagctgcac ttcccttttt tatgtttctc
    13921 cactaatcga aatttagata gacatctaga aatcgcaatt ccaaaaaatt ctgtgtattg
    13981 gatacgcatc ttttcttatt ttttcttatt tcttccatat gggtgtttat ctcagagatg
    14041 atagaatagt ttctaggata aaaagtagca gctacgatgt tatccttcgt agaggattcc
    14101 tgcttcagct agtttttgtt tgatgttttt cagcatatgc ttttctgggt agcggatcgt
    14161 atgcagatgg atgccgcctg ttaattcgga taacaaagta gcattacttt tttgataacg
    14221 tctcataaag gaatcgatat cttgtggcgt tttgatgtgc aaagttcctg ctaactcacc
    14281 gtaaagggga tgttccacaa ttacatcaat gatttcacca ccattttcaa cgattagtct
    14341 cagttcttct tctgtttgat cttttccatg ctggacagcg attttactga tgtgcccagt
    14401 attggtttct tctgtttcga ggatataacc tcttgctgtt gccacgatac ttttacctgc
    14461 agctcgtagt aaagcaatat cgcctacaat gatctgacgg ctgactccaa aagttttcgc
    14521 aaacttgcta gcactcactg gtttttctgt atttgccaag gtttgcatga tttgttttcg
    14581 ccgtgtctct ccttcgatca cacttattcc tccttcattt tgttttggta ttcgcgcgag
    14641 tgtttcatca aatcaacata cgtgtctgtt attgtttcct tatatccctt atttttcagc
    14701 agactaacaa ctttatccaa tacttgctgc tctctttttg catcataaac tgcttggttt
    14761 tcttcttttt tcacttgtcc tattttagtt accgtatcca ttcttttttc taataaagca
    14821 accaactctc gatcgatcat atcgatctta tgacgtaatg tttcaagaga ctcttctgtt
    14881 agttcttcct ctactgtctt gatgatacga gcaggatttc ctgcaatcac tacattgtcg
    14941 ggaaaagatt tggtcacaac cgaacctgcg ccgactacta cgttgtcccc tagagtgaca
    15001 cccggcgtga cgataactcc tccgcctaac caaacattat taccgatttt aatgggtttt
    15061 gcgtactcca gaccgctgtt acgtttcact ggatgtagtg gatgcgtcgc cgtgtaaagc
    15121 tggacattcg gagcgaacat gcagttatca ccgatttcaa tcgtgctgac atctaaaaat
    15181 gtacaattga aatttgcata aaaattctcc ccaacaaata tattatatcc gtaatcaaaa
    15241 ttgataactg gctccatata gatttttttc cctgtacggc caaaagtctc tttcaacaat
    15301 tgggatcgca gttccgctga ttctgcttgg ttgatgattt ggctttgact tcttgcaaac
    15361 atacggtcgg caaccagctc aggatcattg gcaaaataaa gctcgcctgc aatcattttt
    15421 tctttttcgg ttttgaattc agtcataatc tgctcctaat tcttgtattt ttttgaaaac
    15481 aaccgctccg tcttcttgaa taaacgtata aggctccgct aaataaacag gcgtttgatt
    15541 gattactaag attttggctg acggctggcg aaactgtacc agatcacaaa acggatgaac
    15601 ttgaaaactc gttccaacaa tgacgatcag ctctgcttgg ctgacggcat aagccgcctg
    15661 ctccatgact ccatcttgaa acccttcacc atataacacg atgtctgggc gtatttgtcc
    15721 tccgcatgct tgatgtttgt cgctgtgaag atattctttc cagtctaccg tttgttgaca
    15781 ttttcgacaa tgacattgat acagactccc atgaaaattg acgagttgcg ggcttttagc
    15841 ctttgcgtgc aaaccgtcga tgttttggga aacaacccaa acattttttt ctttcgctag
    15901 atgtgctatt tccaaatgaa tgatattggg ttttgcttca ggatgataaa tctttttgac
    15961 aaattgatag aatttatccg gttcattcac cattgcttga tgactcagca agtattccgg
    16021 ctgctccatc ccgtggtaga ttcctgacaa agatcgatag tctggcactc cagaaggtgt
    16081 ggatacccct gcaccggtca aaaatgtaat ttgctgataa gctaatatct cttttgctgc
    16141 ttcgtctact gttaattgtt tcatgttttg tcacctcact ttccattata gcccatactc
    16201 gaacaaattt attgttgaaa agtcagaaaa atgggaataa aaaagtgtta tgggcatttg
    16261 agcaatttat cacaatgttc actgacaaaa gaatgaaaac gtgttacaat actgctgtat
    16321 aaaagtgagg tgaatcgata tgttaccatt ctttatttat tggggcgtta ttctggcttg
    16381 tatcgcctgg ttagcgttaa gcgtttattt ctctgttttt tatcttgtac gcaaagaaaa
    16441 tggaaatctt tgggcttttg ctttattcaa cgtgattgcc gcaattgttt tagccattac
    16501 attagccgta tatcgaacat ggggctgggg aatcacccaa tactcaagtt tgatttatct
    16561 aatattagcc atttatggcg tcgttgttct tttgcaagcg atcctaggac gtgagccaaa
    16621 gaaagcggca gcttaaatga tcgagatgga actggacaca ggaaattttc tgttctagtt
    16681 ctttttttat tgtattacat acatttttgt gtataattat actatcgttt gcaatggata
    16741 gatgattcac attttgattg ttttttcggt aaaagtaaag atttctatat ttatcagtat
    16801 tttttcaaaa accagcagtt atagattgac aaagcctctc gagtatcgta aacttttttt
    16861 gtataaataa ctgaatacga gatgcctcaa tcaatgaggt agaggtcgcg agaatcatta
    16921 accctgtgga gtgaagcaaa catggagaag caggaatagg gaggatcgcc gaaatgtgca
    16981 cttttttgct tggagtgtat gttgggacgc aagttaagag cttgcggact gtcttagcag
    17041 tgatgctaag ttgcgctatg ttttgtgaga ggtttatcac cttatggaat tttagcggcc
    17101 tttttatatc tataaatcgg tcgcttttta ttttttcaga taaagcttta ccgcatttca
    17161 tctagaccgc tcgtgtatca tataatcaga aagggagaaa aaaatgaaaa aaaatatggt
    17221 ttcagttgcc gtactatttg cttttgtctt ctcactattc agtgtgggat ctctggcaaa
    17281 tgcagatgaa aaaacaccga gcgatgagtt tcgcgtaggt atggaggctg ggtatgcacc
    17341 cttcaactgg tctcaacaaa cagatgagaa taatgccttg ccaattcaag gccaaaatag
    17401 ttacgccggc ggttatgatg ttcaaatcgc taaaaaagta gcagatggtt tagggaaaaa
    17461 attagtcatc gtccaaacga aatgggatgg tctggctcct gcccttcaat caggcaagat
    17521 cgacgcgatc atcgccggaa tgagccctac tgctgaacga cgaaaagaaa ttgattttac
    17581 cgatccttat tacgaatcac aattagtagt agtagttcaa aaagatagca aatatgcaaa
    17641 agcaaaaagt ttagcagatc tttctggagc aaagatcact gctcagttaa atacattcca
    17701 ctatactgtg atcgatcaga taccagatgt gcagaaacaa caagcaatgg ataatttctc
    17761 agcaatgaga acagctttag cttctggaat gatcgatggt tatgtcagcg aacgtccgga
    17821 aggtgtaacg gcaactagtg tgaacaaaga cttggaaatg cttgaattca gcggtgcaga
    17881 tggcttccag acaaacccgg aagatgttca gatttctgtt ggtatgcgta aaggcgaccc
    17941 ggatatccaa aaagtaaacc agattctttc tggcatctct aacgatgaac gaaccaaaat
    18001 tatggaccaa gcagtcaaag atcagcctgc tgcaactgat agtgaagaaa aacctggcat
    18061 catttctgat ttcactacga tttggaaaca atacggcaat atgttcttac gaggtgctgg
    18121 acttaccttg ttcatcgcat tgatcgggac agttgtcggt actacacttg gattactgat
    18181 tggtgtcttc cgttcggtac ctgaatcagg aaatgcactg actcgcttct tccaaaaaat
    18241 cggaaatgca cttctttcta tctatgtcga agtattccgc ggaacaccga tgatggtaca
    18301 agcaatggtc atcttctacg gtctggcact cgcttttggc atttcattga accgaacgat
    18361 tgccgctttg tttattgtct ctctcaatac tggagcttac atgacagaga tcgttcgtgg
    18421 ggggattttt gcagtagatg aagggcagtt cgaagctgcg caagccattg gtatgaccca
    18481 tggccaaacg atgagaaaag ttgttattcc acaagtttta cggaatatct tgccagcaac
    18541 aggaaatgag tttgttatca atatcaaaga tacagcagtt ttaagtgtaa tcggcgtagc
    18601 tgatttgttc ttccaaggaa actctgcttc cggtgcgaat ttccaattct tccaaacatt
    18661 tacgatcgtc gggatcatct acttagtcat gacctttacg atcaccagaa tcctgcgggc
    18721 ggtagaaaag aaaatcgatg gtccttcagc ttataccaag cttgaagaaa tcgacaattc
    18781 aaatttgcag gaataaggag gaagaatcat gaatgaaaaa attattgaaa tcaaacatct
    18841 aagaaaaagc ttcggtgaaa atgaagtatt gaaagatatc agcgtaacag tcaataaagg
    18901 tgaagttgtg acgatcatcg gttcttccgg ttctggtaaa tctactttct tgcgctgtat
    18961 caatttactg gaaaaaccaa caggcggcga gatcatttat aatggagaaa atgtattagc
    19021 tcctaaatat aatttaccta aatatcgaac gaatctaggt atggttttcc aatccttcaa
    19081 cctgttcaac aatatgaatg tcttggaaaa ctgtatgtct ggtcaaacaa ctgtcctgaa
    19141 acgagataaa gaaactgcca aaaaagtagc aatcgaaaat ctggaaaaag ttggtatgga
    19201 tcgttatatc gaagccaaac cagcacaatt atctggtgga cagaaacagc gtgtcgcaat
    19261 tgctcgggca ttatcaatga atccagatgt attgctattc gacgaaccta cttcggcgtt
    19321 agaccctgaa atggttggcg aagtgcttaa tacgatcaaa gacttagcac atactggctt
    19381 gacaatgctt atcgtgacgc atgagatgga atttgcaaaa gatgtatctg accgtgtcat
    19441 ttttatggat aaaggtgtga ttgcagaaga aggtacacca gaagatatct ttgtccatcc
    19501 gaaagaagaa cgtacaaaag aattcctttc acgtatttta aatgcttagg atcaatagct
    19561 gattgggaca aagagctgcg gtgtttaccg cagttctttt ttatgaatta ctcataaaag
    19621 gtaaatttct gaaaattttg gaatcttagt tgtgtttttc agaaaactct cttaatatgg
    19681 aagtaattaa acttttttag ttaggatgat ttaaaaatga atgaagagca agaactgcag
    19741 cgtggactga aaaatagaca tgtccaactg atttctattg gcggagcgat tggaacgggg
    19801 ttgtttttag gttccggaaa gtctatccag ctagctggcc cttcgatatt gctcgcctat
    19861 cttattactg gatgcatctg ttttttcatt atgcgtgcgt taggcgaact tctgttatca
    19921 aacaccaata accattcttt tctggatttt gttgcagaat atcttggcaa aaaagcggct
    19981 ttcatcactg gctggacgta ttggttctgc tgggtatcga ttgcaatggc cgatttaaca
    20041 gccatcggaa tgtatgtacg ctactgggcg cctggtgttc ctcagtggct tcctgaactg
    20101 attgcattag gtcttcttct ttgcttgaat atggtggctg tctcattgtt tggtgagtta
    20161 gagttttggt ttgcattgat caaagttgta gcgatcattg cctttatcct cgtaggagct
    20221 tatatgatcc tcacccacta tacgacagat attggcacag ccagtatcac gaatctatgg
    20281 tctcatggcg gattctttcc aactggtgca aaaggattta tcttagcttt ccaaatggta
    20341 acctttgctt ttgctgggat agaattagtt ggattagtcg ctggagaaac agaaaatcct
    20401 gaaaaagtgt tgccggaagc tatcaataat attcctatca ggatcatctt attttactta
    20461 ggatctcttt ttgtcattat ggcaatctat ccatggaaca gcttgaatcc agataacagt
    20521 ccatttgtag aagtattttc ggaaattggg atcacgattg ctgcttcctt gatcaattta
    20581 gtcgtattaa gtgcagcagc ttctgcctgc aacagtgcaa tatatagtac aggacgtatg
    20641 cttcgttctt tagcgcaaga aggcagtgcc cctaaaaaat tcaaaaagtt aacaacacat
    20701 catgtgccag ggaatgcttt gacattttct actatcgtca ttttcatttc tgttatcttg
    20761 aattacgtga tgcctagtga agtattcaca ctagtaagca gtattgccac tacttgtttc
    20821 ttgtttatct ggagtatgct tgtctacacg cacatgaaat atcgtaaaag tatcctcggc
    20881 aaaaaagctc attcatttaa aatgccgttg tacccattta gtaattatct ggtatttctt
    20941 tacatggcct ttgtgtgtgt cgtcttgttc ctaggaaaag atacgagaat cgctttatta
    21001 ctaacacctg tgtggttctt gcttctatta ctaatctatc atatgaaata tgaacgtaag
    21061 aagtaagaaa gtagttgtga cagaagtggt cagcttcgag aaataagaag gaatttacga
    21121 aaattgcttc ttaaattttt gtgaaatttc ggcttatttc ttggagataa aagcttctgt
    21181 cccagcctct tttctatttc cccggaaaca accgctgata ataggcatct gcttttttat
    21241 aggaaaaaat ccaaatacca gatacaaaaa aacgtccgaa taacccgatc aacggtacaa
    21301 aaaagacacc ggcaatcaag cataagttaa tcatcatgga gataagactt cgacggtcct
    21361 ttgacaaaac tctcacgaca tgggaatctg gtttatttgc tttcatgatt tgcctcatca
    21421 agataccaaa agaaaaactc cacatcgtgt atactaagac ataagccatc tcaggccaaa
    21481 aagcttcagg gttacgcccg acccaactag taacagaagg acataaagtc agccaaaaga
    21541 gcaaaaagat attcgaccag accactcggt aattaattcg ttctgccatc tgaaatatac
    21601 gatgatggtt cacccagatc accgcaacaa atataaagct gatggtataa gctaataatg
    21661 tatttcggat gacccataaa gaatgaaagc tagatgtatt agggatcttg atctctaata
    21721 caataactct caaaacaatg gctaccactc catcagtaaa tgcttctaat ctcgactttg
    21781 tcacgatgat tcctcctcag atgattcagc gcttttattt tatcacttac gttattaaat
    21841 aaccatcgtt ttatataggg aattttctga aaattgttgc atatttttcg aaattttgtt
    21901 acttttaaaa aa
//
LOCUS       DAYYCY010000030        21900 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000030 DAYYCY010000000
VERSION     DAYYCY010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21900
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.030"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            65..1234
                     /locus_tag="ACHAZB_000929"
     CDS             65..1234
                     /locus_tag="ACHAZB_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8613620.1"
                     /translation="MKLRANIIEKRGTLQVVVSYKDSNGKIRQKWKDTKLKKKGNKKR
                     AEKIRDEFLLELESQLAMECECANPNLLFYDYLLNYLEVSKKQVAFNTYVGYKHYVHN
                     RIYKFFYPKKIKLNRLKPYHLQEFYQYMLNDNCTANTVIHYHAFIRKALQEAVITELI
                     STNVADKVRKPKKKQFVSQVYNQQEILKLLDILSEEKLYLVVLLTAFYGLRRSEVLGL
                     KWSAIDFLNKKIMINHVIIENPENMSQLIKKDQTKNSSSYRTLPLVETIERALVNQAK
                     WQQDNRILLGDDYILEDNEYVFTMEDGRLMKPQYVTHRLSKLIKKNGLKKIRFHDLRH
                     SNASIMLDSGQNMKSIQEWLGHASYSTTANLYTHLTAGVKDRAAEALENAFGFKE"
     gene            1808..2377
                     /locus_tag="ACHAZB_000930"
     CDS             1808..2377
                     /locus_tag="ACHAZB_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HFM8613621.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            2364..3224
                     /locus_tag="ACHAZB_000931"
     CDS             2364..3224
                     /locus_tag="ACHAZB_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFM8613622.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            3414..4118
                     /gene="yycF"
                     /locus_tag="ACHAZB_000932"
     CDS             3414..4118
                     /gene="yycF"
                     /locus_tag="ACHAZB_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator YycF"
                     /protein_id="HFM8613623.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            4123..5958
                     /gene="walK"
                     /locus_tag="ACHAZB_000933"
     CDS             4123..5958
                     /gene="walK"
                     /locus_tag="ACHAZB_000933"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HFM8613624.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            5955..7271
                     /gene="yycH"
                     /locus_tag="ACHAZB_000934"
     CDS             5955..7271
                     /gene="yycH"
                     /locus_tag="ACHAZB_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system activity regulator YycH"
                     /protein_id="HFM8613625.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            7272..8141
                     /locus_tag="ACHAZB_000935"
     CDS             7272..8141
                     /locus_tag="ACHAZB_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HFM8613626.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            8196..9005
                     /locus_tag="ACHAZB_000936"
     CDS             8196..9005
                     /locus_tag="ACHAZB_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFM8613627.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(9108..9737)
                     /locus_tag="ACHAZB_000937"
     CDS             complement(9108..9737)
                     /locus_tag="ACHAZB_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFM8613628.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            9907..11199
                     /locus_tag="ACHAZB_000938"
     CDS             9907..11199
                     /locus_tag="ACHAZB_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8613629.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(11275..11937)
                     /locus_tag="ACHAZB_000939"
     CDS             complement(11275..11937)
                     /locus_tag="ACHAZB_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFM8613630.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            12162..12446
                     /gene="groES"
                     /locus_tag="ACHAZB_000940"
     CDS             12162..12446
                     /gene="groES"
                     /locus_tag="ACHAZB_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFM8613631.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            12497..14122
                     /gene="groL"
                     /locus_tag="ACHAZB_000941"
     CDS             12497..14122
                     /gene="groL"
                     /locus_tag="ACHAZB_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFM8613632.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
     gene            complement(14211..15350)
                     /locus_tag="ACHAZB_000942"
     CDS             complement(14211..15350)
                     /locus_tag="ACHAZB_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8613633.1"
                     /translation="MWVEQTKDGQFKFIERYVDPYTEKTRKKSTTLTSNSPQAWKKAQ
                     KILDKKIKEALEDYNKSDITFGELYKEWYEYYKQHVKRTSYLKVPMMMKHVSKHISDD
                     TIVRNIDETLINKIIEDMYTFGDLSLNYTKQTKTTLSVMLNYAIDRKYIQRNPALAVK
                     IHPKKVEEEKRRLSMNKKYLEKEEIDQILKQLYSNPRRKLHGIIAEFLYLTGLRYGEL
                     LALQMKDYEDGKISINGTLDYTSVKMDNAIKTTPKNTYSQREVQLPNRAKELIESVIA
                     DNILAGRPTDPDQYIFISTSGTPLALHSFNAILHKVEEELELEKSLSSHIFRHSHVSL
                     LSELGVPLKAIMERVGHSDANTTLSIYNHVTKRAKQQVIDKLNSL"
     gene            complement(15461..16045)
                     /locus_tag="ACHAZB_000943"
     CDS             complement(15461..16045)
                     /locus_tag="ACHAZB_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HFM8613634.1"
                     /translation="MAKKVMGQDGKMYKVSKPFYKKVWFWVLAVILIIIIGSALNGGS
                     DSNKASDNGGEKVTKSSTSASSSKEEKSDTFYKIGDTVKVGDAEYTLNSVELTDERNQ
                     FEENQPAQVVKITYTVKNDGDSDIPVGTDVEVYGPDDKKSETYANENTMGSVAPGKQM
                     DVTAHFTLNQKGEIEIHFSPLVSFEKAAIFKATV"
     gene            complement(16172..16603)
                     /locus_tag="ACHAZB_000944"
     CDS             complement(16172..16603)
                     /locus_tag="ACHAZB_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFM8613635.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFTASHFEYSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(16621..16941)
                     /locus_tag="ACHAZB_000945"
     CDS             complement(16621..16941)
                     /locus_tag="ACHAZB_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8613636.1"
                     /translation="MNTYEIIKELAKEKGLSIRQLEMNFGYSNGYLGSWKRQTPNSIE
                     LARLADYFGVSVDYLLGREERETPKHVDLSENDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            17241..17381
                     /locus_tag="ACHAZB_000946"
     CDS             17241..17381
                     /locus_tag="ACHAZB_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8613637.1"
                     /translation="MEKTVSAKIEDLKLDILKQAKVAMEHAVDKEDSAMVAAIAEILA
                     HV"
     gene            17679..17936
                     /locus_tag="ACHAZB_000947"
     CDS             17679..17936
                     /locus_tag="ACHAZB_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HFM8613638.1"
                     /translation="MNKEMSQTIKVQKMIDDLTHGIDSQADKIIKELQGQKVKDAKML
                     LKTINFEMNPTKRKLADVLEEKLASAINEQELLFETDTFNS"
     gene            complement(17907..18215)
                     /locus_tag="ACHAZB_000948"
     CDS             complement(17907..18215)
                     /locus_tag="ACHAZB_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8613639.1"
                     /translation="MKPEFVITMLDGEKIKIFADTLVVGYDDASNIEEKDGMFYSSQI
                     YVNTLQGDIGVSTLATSRPLVGLIGFLSKATFFSLGDDIDSDETVIYKTSAVKSIGFK
                     "
     gene            18293..19039
                     /locus_tag="ACHAZB_000949"
     CDS             18293..19039
                     /locus_tag="ACHAZB_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFM8613640.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVADVLGYSNPQKAIR
                     DHVDLEDKTQNDSFTVNGTAVVLINESGLYSLILKSKLPSAKKFKRWVTSEVLPTIRK
                     TGSYSNVPQSFAQALRLAADLEEKNQLLEQQIAEYEPKISYLDTILSSTDTVATSQIA
                     ADYGMSAIALNKLLNELGVQHKVSGQWILYRKHMNQGYTKSHTSEIPKADGGTKVVMN
                     TKWTQKGRVFIYNLLIAEGYYPQMDLEEIG"
     gene            19054..19257
                     /locus_tag="ACHAZB_000950"
     CDS             19054..19257
                     /locus_tag="ACHAZB_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8613641.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            19362..19661
                     /locus_tag="ACHAZB_000951"
     CDS             19362..19661
                     /locus_tag="ACHAZB_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8613642.1"
                     /translation="MKKPTLSELIEAAEKAVKPDDWYRQSLILEKFHGMSKTTLVEYC
                     KEMETIPEFAEGILRPGHSTTFIHYHTFIWFLKWKDANKYRVKSLSPSDVLKEAS"
     gene            19661..19843
                     /locus_tag="ACHAZB_000952"
     CDS             19661..19843
                     /locus_tag="ACHAZB_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8613643.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEHRAKKASREGLTKI"
     gene            complement(19849..20325)
                     /locus_tag="ACHAZB_000953"
     CDS             complement(19849..20325)
                     /locus_tag="ACHAZB_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8613644.1"
                     /translation="MRKKLNYILTATLTIYIILFITLSFLSSIKKIPFITLNKNSILK
                     EFNNTDYIAFLKLFSVCVLISCVIIFIVAKTCWTMLNKNQRDVENIAIFLIKITSSIS
                     IIIPMTVTFSKEQFNVAATYLSFIALFSFVLPKRNKSNFDNKELDEVRNSSPKEQK"
     gene            20378..21103
                     /locus_tag="ACHAZB_000954"
     CDS             20378..21103
                     /locus_tag="ACHAZB_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HFM8613645.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFKKKHKHVLEAIESIKRSVENS
                     ANVEDGSNFGQMFVEGNEPDSYGRSRRVYFMNRDGFSLLAMGFTGSKAINFKLKFIEA
                     FNEMEDVIRKNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNM
                     KVMEVLGGKESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLP
                     ETAIRMEIDQLNRQQRLFGDENE"
     gene            21277..21618
                     /locus_tag="ACHAZB_000955"
     CDS             21277..21618
                     /locus_tag="ACHAZB_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8613646.1"
                     /translation="MNKELSTLDQYLTDSEWGKSNIKETNNRKIRRNLLTNEELSCDQ
                     DDLGNFVSIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYNRDNLIDISYYGSGKK
                     YIAEMEAEFDE"
     gene            21611..>21900
                     /locus_tag="ACHAZB_000956"
     CDS             21611..>21900
                     /locus_tag="ACHAZB_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFM8613647.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTET"
BASE COUNT         7455 a         3638 c         4380 g         6427 t
ORIGIN      
        1 aatcatgcta taataaaata gtcaaaattt ttgccgatat ttcagtttaa gaggagagat
       61 tgaattgaaa ttaagagcaa acataattga aaaaagagga acattacaag tagttgtatc
      121 ttataaagac tctaacggaa aaattagaca aaagtggaaa gatacaaaat taaaaaagaa
      181 aggaaataaa aaacgagcag aaaaaattag agatgaattt ttactagaat tggaaagtca
      241 attagctatg gaatgtgaat gtgctaatcc aaatttattg ttttatgact atttgcttaa
      301 ttatttggaa gtatcaaaaa aacaagtagc atttaacaca tatgttgggt ataagcatta
      361 cgtccataat cgaatttaca agttttttta tcctaaaaaa attaaactaa ataggttaaa
      421 gccttatcat ttacaagagt tttatcaata tatgttaaat gataactgta cagcaaatac
      481 ggttattcat tatcacgcat ttatacgcaa agctcttcaa gaggctgtta ttacagaatt
      541 gataagtaca aatgtagcag ataaagtaag aaagccaaaa aagaaacaat ttgtttctca
      601 agtttataat caacaagaga tcttgaaatt acttgacatt ttgtcagaag aaaaactcta
      661 tttggtagtt cttttaacag cattttatgg attgcgaaga agtgaagttt taggattaaa
      721 atggtctgct attgattttt taaacaaaaa aatcatgata aaccatgtaa tcatagaaaa
      781 tccagagaat atgtcacagt taataaagaa agatcaaaca aagaactctt ccagctatcg
      841 tacattacct ttggttgaaa cgattgaacg agcattagta aatcaagcta aatggcaaca
      901 agataatcgt attcttttag gagatgatta tattttagaa gataatgagt acgtatttac
      961 tatggaagat ggacggttga tgaagcctca atatgtaacg catcgtttga gtaagttgat
     1021 taagaaaaat ggattgaaaa aaattcgctt tcatgacttg cgtcattcta atgcttcaat
     1081 aatgttagat agtggccaga atatgaaatc cattcaagaa tggctgggac atgcatcgta
     1141 ttcgacaact gctaatttat acacgcattt aaccgcagga gtaaaagatc gtgcagcaga
     1201 agcattagaa aacgcatttg ggtttaaaga ataaaaaaac aaaaaacaat atctttgggg
     1261 tatcgcttct tgtttttaga atttttttgt tggcaatttg ttggcagtat gttggcaatg
     1321 aaaaaattca caaactaaaa acctatttat aaagctttat aaaacttttt tcacaaatag
     1381 agcatttggt taagagtcag ctgctctacc aactgagcta acggcccgta caaaaagtac
     1441 ttaataatag tagcactaag aattttaaaa tgcaagacta catttaaaga aaatcagaaa
     1501 aatctttact tccttatagg aaatcattca tagattattc acatttgctc gctattatat
     1561 aagcatacca acaaacaaag ttcttgcaag agaacaaacc ctaacacttt tttcattttt
     1621 tactcctttc ccgccgtctc cccggcgggt ctcctgttct taacgataaa agacaagaag
     1681 gcttagcctt cttgtttttt gttttcatgg aatcccgcta atcaaatcac ctagttaaag
     1741 caactagatt gaatgtttat cataagtctg atatcatgtt tatgaaatga aaaaaggagc
     1801 ggttatgatg gaaaaaatgc gtcagcaatt aatggagtgg gggaaggaac tagaaacatt
     1861 ccgtctgcct cactgggaag aactccctga cttagaactt tatatggacc aagtcagaac
     1921 gttagttgat cgttatcttt cacctgtgat acaaggagaa aagcatccat tgcttacctc
     1981 ttctatggta aacaattacg ttaaactagg tttgatacca gcaccagtta aaaaaagata
     2041 taacaaagaa cacgtagcat ttttacttgc aatcactacg ttgaaacaag tattgactat
     2101 tcctgaaatc aaagaaggga tattgttcca aggaaaaaca gtaggaattc gtgaagccta
     2161 taatctattt tgcgatgaac aagaagcagc tgtgtggatg gtcagccaat tagcacaagg
     2221 taaatctcat ccgcaaaaat tcgaccaggc aacccctgta gaatatattg ctgtaagagc
     2281 ggcaacttta tcgtttgcaa tgaaacttct tgcggaaaaa acgattgttc tagaaaccga
     2341 atatttaaaa gaggagaaaa caaatgaaaa acaataaaat cgcaatttta gtagattcag
     2401 gtacggatgt tccagaagaa ttgataaaaa aatacgagat gtatatgatc ccattgaaaa
     2461 ttatttataa agatcaggta tatacagata aaattgacat tacccctgaa gagatctatc
     2521 aacgtttgcc agaggaaatc cctggtacat ctttgccgga tggtgagtcg atcacagaaa
     2581 tctttgagca aatcaaaaaa gacggttatg aaaaggtact tgcggtaaca atttccagtg
     2641 gattgagcgg gacatataat gtggttcgtt taattgcaca acagcaagaa gaattggaag
     2701 catatgtact agatacgaaa aatattggta ttggtgcagg tttttctgcg atccaagcag
     2761 caaaatggct ggaagaagga gtagaatggg atcaactgat cagtaatctg aatgagttag
     2821 tcgaaagaac aaaagttttc tttaatgttg cgacattgga atatcttcaa aaaggtggac
     2881 ggatcggttt agttgcttct attgtaggaa ccgctttaaa gcttaaccca atcatctcat
     2941 gtaatgaaga agggatttat tacacagtag gaaaagctag gggacgtaaa aaaagtttgg
     3001 ataaaacggt tgcttacgtc aaagaaaaaa tcggtaatgc aaaggtcttc aacttagctg
     3061 ttgcccatgg ggatgcgaaa gaagaagcaa ttgaaatgga agcgagactt aaaagagagt
     3121 tcccacaagc aaaccaaatc tactttggcc agatttctcc ggcattagta gtccacactg
     3181 gtccgggatt attgggtgtc ggtgtacaag ttttagaagg ataaatcaaa aaaaggaaac
     3241 actgaggtga aaatcagtgt tttccttttt tattgaagtt agaacaaaaa ataataaagt
     3301 attttgcact ttcattttaa ttttctcttt tgttttatag gcaaagatga aacaaaagca
     3361 gaaatacggt ataatgagaa ggtatgacaa taatatataa gaggtggctc tttatgaaaa
     3421 aagttttagt tgtcgatgat gagaagccga tttcggatat cgtcaaattc aatttagcca
     3481 aagaaggcta tgacgtttat actgcatatg atggggaaga agccttggaa aaagttgctg
     3541 aggtagaacc tgatttgatt ttacttgatt tgatgctacc taaaatggac ggcttagaag
     3601 ttgctcgtga ggtacgcaaa acttatgata tgccgatcat tatggtaaca gccaaagatt
     3661 ctgaaattga taaagtccta ggcttagagt taggcgccga tgattacgtg accaagccat
     3721 tttccaaccg agaattagtt gcacgtgtca aagcaaatct tcgtcgcggt gccacggctg
     3781 cgaaggaacc agaagaggca gcacctgcgg aattgacgat tggtgatttg acgatccatc
     3841 cagaagctta catggtgacg aaacgaggag aaacaatcga gttaacacat cgtgaattcg
     3901 aactactctt ttacttggcg aagcaccttg gacaagtgat gacaagagaa catttattac
     3961 aaacggtttg gggttatgat tattttggag atgtacgaac agtggatgta actgtacgtc
     4021 gtttgcgtga aaagatcgaa gataatccaa gccatccaaa ttatttagtg acacgtcgtg
     4081 gtgtcggtta ctatttgcgt aacccagaac aggagtaatt ggatgaaaag aaaagtccgc
     4141 ttttttcgat cagtcaattt taagatcgct attaccttta ttctgatttt attgatctcg
     4201 atcgaaatta ttggtgccta ctttatacgt ggtttagaac gttccacgat caatacattt
     4261 attaaagata tgaatcaaac ggtggaatca ttagcaacaa caatcagccc ggaattgaat
     4321 cgaaaagata atgcagatga tgaagaggta aatgcgaata tcaaaagatt tattgaaaac
     4381 agtgcgacta gcgatatcat cgaaatacgg gtcgttgacg aaaaagggat catccgagga
     4441 acaaccgatg tcaatgaaca aagcgctgtg ggaaagaaaa atgattatgt tgatatcaat
     4501 gatttcacga ctaaacgtta tgttgcttta gataacgacg ataaacgggt gaatatcaat
     4561 gttcaaccga tcctgtcccc gaccggcgat gccgttatcg gggctcttta cgtcaaaagt
     4621 aatatcgaac aaaaatattc ggaaatcaat aacacagcag tcattttctt tactgcctca
     4681 ctaattgcgg catttatttc aatgatcgta tctgttttag ttgctcgttc gattacacag
     4741 ccgatcggtg aaatgagaga acaagccatt cgaatcgcta gaggagacta cagtcggaaa
     4801 gtcaaagtgc atggacaaga tgaacttgga cagctggctg ataccttcaa tcagttagct
     4861 gaacgtattg aagaagctca agatacaatg gaagctgaac gcaatcgttt ggatagcgtg
     4921 ctatcccata tgacagacgg cgtcattgcg acagatcgca ggggtaaagt cattacgatc
     4981 aatgatatgg ctgtttcctt gctagatatc aaaaacgagg aagcaatcgg acaatcgatt
     5041 ttgacacttt tagatatcga agaagaatat acgttgcgga aattattaga agatccaaac
     5101 gaaatggtac ttgaccgttc aaccagtaca ttggaaagtg atcagatgat cttgcgagta
     5161 gacttttcta tgatccgtcg cgaatcaggt tttatcagcg gattagtagc tgtcttgcat
     5221 gatgtgacag aacaagaaaa aacagaacgg gaacgtcgtg aattcgtctc taatgtttcc
     5281 catgaacttc gtacaccgtt gacaagtatg cgtagttata tcgaagcatt gagcgaaggt
     5341 gcgtggaaag acgaggaagt agcaccaaat tttttgaagg taacgctaga tgaaacagac
     5401 cgaatgattc gaatgatcaa tgacctctta aatctatctc gcatggatac aggtaatacc
     5461 cagcttcaat tagaatatgt caatttcaac gagttagtga attttgttct tgaccggttt
     5521 gatatgatgg tgggaaatca ggaaaaaaat tataagatcc gtcgtgaatt tacgcaaaga
     5581 gatctttggg tcgaagtaga tacagataaa atcatccaag tagtcgataa tatcatgaat
     5641 aacgcgatca agtattcacc agatggggga acgattactt gtcgcttatt agaaacgcat
     5701 aataacgtga ttttaagtat caccgatcaa ggattgggga ttcctaaaaa agatctgaac
     5761 cgagtgttcg agcggttcta tcgagtagat aaagcacgtg caagagcaca aggtgggaca
     5821 ggacttggat tagctatttc tagagaagtg attaaggcgc atcgaggcgc tatttgggca
     5881 gagagcaagg aaggaaaagg gtcgactttt tatatctcgc tgccatatga accttatgag
     5941 gaggaatggt gggaatgaga atctctgaaa aagtcgttcg tataggactc attttgatgg
     6001 ttgcgttaag catatacttc tcttatgcaa tctggctgag cccagcagga aaaagttcaa
     6061 tcaacgtaga tgagacaaac actcaaatga tcgagtctca aagcaatcga aaaacttccg
     6121 aagtctttct tccgcttcat gttacatggc accattcggg aatgatccaa gaaaccaaca
     6181 gtgaaaatct cgtgtaccga ttgcaattga tcatcgataa agcaagattt ggaaagcttt
     6241 ctgaagtagt ttcaggagac aaatcacagt ttgaacaggt gaaaaatctg aactctggtt
     6301 ttgagttggc ctataatgcg ccttttttgc taagagaata caaggaagca tttgatttag
     6361 atttagatgt gtcgaacttg gcaaaagaaa gcgaaaatat gtattttact agaatacaat
     6421 tcgatcaagg aaataaaaaa atccgatttt tgaattattc gaaccatctc gtatatgaag
     6481 caaatatttc gttgaattgg acagatatca ataaagaatt aacagcaaaa gacacaaaat
     6541 ggacagaaat gacaacagaa ccatcgacga tcgacacgca atatgctacg aaacattcga
     6601 tcaaactgaa gaagtatagt tatatcctgt cacagcaacc ctatacactt tttagaaatg
     6661 cttttttcca gaaaccagat agcgtgaaaa ataatgatga aagcaatgaa ttgatttttt
     6721 acggtgggaa tgaaacaatg tctatccatt cggaaacgca agtagttgat tttcgtggag
     6781 agctgccaga gaaaaaagaa aatgctagtg tattttctga aagtttcccc tatattagca
     6841 aattaggcag ttctttaggg aacctcagat attttgaccg cagtgacaat acgatcgatt
     6901 atcggatatt cgtggaaggc ttcccggttt ttgggacaga cagcagagga cagattggtg
     6961 tggaagttgg cggtgaaacg aatggttctg tgcaagtgaa tatccaaacc agtttgaata
     7021 cgattcaagt gccgatacct tcagacgaag aagtagaact tgcaagtacg aatgaaatca
     7081 tccagcagtt aaaagctaag ggagcagata gcaacaagat tggttcagtg atcgttggct
     7141 atacatggca aaatgtcaaa gaaacgaatc gtgtcgttga tctcttacct gaatggtatg
     7201 tgaaatataa agataactgg tattcagcta acgaccttct ggaaaaatta cctggattgg
     7261 aggcgaatta attggatttc aaaaaaatcg aatggatatt tttcgtcgct tttttagggt
     7321 tgaatgtgtt tttattcagc agttatcatg acgcggtata ccaagaaaac aatgtcattc
     7381 gctctaatca gactgaatca atccaacaac gtctggctag cgacgatatc acttacacgg
     7441 ggaagatttc gtctgagaac aagcaaggct attatcttag cgcggagcag accaatctta
     7501 gcacttcatt agcgaattat cgcaaggctg gtaaaagcgg cttgctggca agtggtgtgt
     7561 ttttagacgg tgatgttttg acgaaatctt tatccagttc agatgctgaa aagaacgtga
     7621 tcgatccaga taaaattagt agttccttgg aagatttttt atctaacaaa gacaatattt
     7681 tattcggtaa tgattatgtg tacacgaaaa acttttctca tttagaagaa gaaccactag
     7741 aaatccaagc aagccaagag tatgaaggca tcccattcaa tgatgataca gcaaaaatcg
     7801 ttctttcttt agaaaaaaac gataatgatt atagcatcac gaaatacatg cagacgcatt
     7861 tatctgatat cgagcagctg cgcgaaaaga cggaactgta tacggaagag gaagcaatca
     7921 atacattgta catcaataac aaaataccaa gaggatcaaa aattttatgg cgtcagctag
     7981 cttattcctg tattctgaaa gtccgggaaa aaaatgtgta tgttcctgtt tggcatgtag
     8041 cgatcgaaac aagcgataaa gtggtccaaa ttgagagcgt caacgctttc agcaatacga
     8101 tcgtgacaaa caatactgta ccaaaagtgg aagatcaata aaaaagcgag tataatgaaa
     8161 aaagtttaca aagtaagaag ggaagtagga gtcaaatggc tgaagaaaaa ccagctttta
     8221 aaatcagtat cctagcaagt ggaagttcgg gtaattcact ttatatcgaa agtgaaaaaa
     8281 aacggctttt ggtcgatgca ggacttagcg gtaaaaaaat cacttccttg atggctcaaa
     8341 ttgatcggac accggaccaa ttagatggaa tacttgtgac gcatgaacat cgagatcata
     8401 tacatggcgt gggtgtcttg gctcgtaagt atcatttgga tgtttttgcc aatcagcaga
     8461 cttgggaagc gatggaacca ctgattggaa atgtaccagt ggaacaaaaa catctatttg
     8521 agatggggaa agtcagaaca ttcggcgata tcgatattga gagtttcggc gtctcccatg
     8581 acgcagcggc tccgcaattt tatcgtttcc ataaagaaaa tcggtcattc gtcatgttga
     8641 cggacaccgg atattgcagt gattatatgc gaggtgtcat tgaaaatgcc gatggttatt
     8701 tgatggaaag taatcatgat cttgagatgt tacgcatggg accttatccg tggaatttaa
     8761 aacagcgtat tttaggagat agaggacatc tttccaacga agacggtgca cttgtcatga
     8821 ctgatgtgat cggtaatcgg accaaacgaa tctatcttgg ccatcttagc aaggaaaaca
     8881 atatgaaaga acttgctcat ttaacaatgg aaaatgtttt gaaagaaaaa gattttggcg
     8941 ttggacatga ttttgaaatc tatgatacag atccagatga agcaactgag ctattttcga
     9001 tttaaacagt tcaatcaata aaaatataaa aagcagaaga taaaatcaat cagggttggt
     9061 tttatcttct atttttattt ttttagcata ttaaaaaaat aaccgtatta tcgactgact
     9121 acactgatgt gctgcttttc atgtttctca tccagtatct catctactaa tgcaatcgcg
     9181 taatctgcca tgctgatcgt actgtcgcta gcagcgtttc tttgtaaata atcatcactt
     9241 aacaaataag tacctgtacg tttgccatgt ggatcaaata aagaagctgg actgatataa
     9301 gtccaatgta gatttttaga ttgtttcaat tttaacaatc cttcgtacat attgaaagct
     9361 gttggataga agggtgcatt tttaggtact tggtcaatca tacgagtcgt cttttctgta
     9421 tctaagaata acgaagaggc acccccaacg acaattaagc gtgtatcggt tccttcaagc
     9481 atctcaatca aatgggacaa agaagtttga tgcatttctt cttttccttt gggagcgttg
     9541 aaagcatcaa cgacaacatc aaaagatgca agatcagtag ttgtcaactc aaataaatcc
     9601 ttttgtataa acggaacatc tgatggcaat ttttctggat gacgcacgat tgccgtcacg
     9661 tctaaatctc tggacaatgc ttcgtctaaa ataaatgaac ctgcatggcc agtagctcca
     9721 atgagtgcaa ttttcataaa aaacctcctt ttttattcct gtccattgta agcagttgct
     9781 gaatattcgt caaagaatcc gatttttgaa aagctcagtg cgcattcctt tcatggttcg
     9841 tgatcattgg gtatagttaa attaagaaaa ctgattgata aaaatagaag ttgaaggaga
     9901 taagaaatga aagagtggtt agatcgtgcc tttttaccaa tgataacatc tgttgtgaat
     9961 cttagaccct taactgcttt gcgtaatgga atgacttaca cgatcccaat aattattgtt
    10021 ggctcgctgt ttttcttatt tgctcatttt cctgtggatt acatcgctca gtggatagac
    10081 aaaacaggat tttctatgta ttttacacaa gtataccagg ctacatttga tgtgatagcc
    10141 ttttggatcg tttttgccat tgcatacgct tatgcaaaag aagaagacat cgatcgctta
    10201 ccagtcggca taacagcctt gtctgcttat ctactgctta ttcagccact aaatactcag
    10261 atagatactg tctggacagg tgctgaaggg atactaggag cagttacaac tgggctcatc
    10321 atcggatggg gctatgcacg tttagtaaga cttgcgaaag ggtggaagca aagagagaag
    10381 atacctgaaa atgtttggca gtcttttact tcattgattc caatcattgt tatagtcact
    10441 tttacgctta tcggttcagc ggtttttcaa catttgttca gaatgactgt tgttcaagga
    10501 gtatggacat atgtccagct tcccattcaa tcgttcatag acacgttgtt tggtgtgctt
    10561 ttattaggtt tttttgttcc gtttttatgg ttatttggtc ttcatggatc tgcgatggtc
    10621 aatgggttgg ttagtcccgt cttgcaagcg aattcattag caaatgcaga aatcttagcc
    10681 tcaggacaag agttgacggt ggcaaatggc ggacatatcg ttactcagca atttctcgat
    10741 cagttcatga cggttacggg tgctggattg acgttagggg ctgtattttt tatgacggtt
    10801 tttgcgaaat cgaagaaata cagagaatta ggaaatcttg ctcttttacc agcctttttc
    10861 aacatcaatg aatcaattat tttttccaca ccgatcgtga tgaacccgat gatggctgtt
    10921 ccttttattt tcgcaccggt cgtttcagga ttgattacct attccgcttt atattttgga
    10981 ttcgttcctt tatttacagc agtccaagtc ccttggacaa caccgccgat cctctcgggt
    11041 ttcttagtag gcggagtgcc tgcagcgatt ttacaaggta tcgtgttgtc aattagtttt
    11101 ttcatttatt tcccattttt gaaaaaaata gattctgcaa actgcaaaaa ggaaagacag
    11161 acaaaaaatg atggagcagc agaaaagatg aacgactaaa aaacaaaaat actcctgtcg
    11221 ctttagattg ggaaaatcac caatctagga tgacaggagt atttatagtc atgtttatac
    11281 tagccctttt tggacagcta attgaacaag aacaactaat gtgttcattc ctacgtgtgt
    11341 gatcatcgat gtccagatcc gaccagttgc tttgtactct aggctgaaga agaaacctag
    11401 gaaaaagtag attagcagat gaccatcatt atgagcaaac gcaaacagca aagagctgat
    11461 cactgctccg acccaaaaat ttgaatatcg actgatgatc cccagtatac tgcggcggaa
    11521 tacgaactct tccatgatgg gtccagcaac agttgtcgcc aaaataaaag cgggtgcttc
    11581 catgatcatc tggatgatat tttgtgtatt ctcggaaggg gttgctttgc caaacaatac
    11641 agattctaac atgacagcga tgctttgcag aatcaatgcg acaaagattc caaccaatcc
    11701 ataaataaca atctttggag cagaagcttt ttttaccgct gattcgatcg ctaagggttc
    11761 acgttgctta aaataaaaaa tagctaggac aacggcgcct agtatataca tgactgcagt
    11821 tgctgtgatc aattcagacg tagattttac aagtcctatc gaagctagga tcaagggtga
    11881 gataaaaatt agaccatagc aaaaaatgct gaggaagcta tattttttta aagacatgta
    11941 attcctcctt ttaagggata acaagcatgc aaggcaagct tatatcgatt gatttcaatt
    12001 atatcataat ttcaataagc agctgtcaga ataaaatttc ttttctttga tttaaaagct
    12061 tgcaaaaaag agggagaatg agtattatta aaaatgtagg ttagcactcg aaggttaaga
    12121 gtgctaacag attaaaaaaa gagttatcgg agggatttat cgtgttaaaa ccattaggtg
    12181 atcgcgtcat tatcgaagtc gcaaaagaag aagaaaaaac tgtcggagga atcgttttag
    12241 cttctgctgc taaagagaaa ccccaaactg ggacagttgt tgcagtcggt gaaggccgtt
    12301 tgttagaaaa cggtgaaaaa gttcctgccg ctgtaaaagc aggcgaccaa gttatgttcg
    12361 aaaaatacgc cggtacagaa gtaaaatacg aaggaaaaga atatctgatc gtcgcaggaa
    12421 aagatatcat ggcgattgtt gagtaaattt ggaagataaa aaaataatca aaaataatca
    12481 atgaggtgaa tcaatcatgg caaaagaatt gaaatttgca gaagacgcac gcgcagctat
    12541 gttacgcggg gtagataaat tagcagatac agtgaaagta acattaggac ctaaaggccg
    12601 aaatgtcgta ctagaaaaat cttacggctc tccattgatc acaaatgatg gtgtgacgat
    12661 cgcaaaagaa atcgaattag aagatcattt tgaaaacatg ggtgcaaaac ttgtatcaga
    12721 agtagcatca aaaacaaatg atattgcagg agacggtaca acgacagcaa ctgttctgac
    12781 acaagcaatc gttcgcgaag gtttaaaaaa cgtaactgca ggtgctaatc ctctaggcat
    12841 tcgtcgcggg atcgaattgg caacaaaagc agcagtcgaa gaattgcata atatctcaac
    12901 tgttgttgat tcaaaagaag ccattgcaca agtagcagct gtttcttcgg gttctgataa
    12961 agttggacat ttgattgctg atgcaatgga aaaagttgga aacgacggtg tcatcacaat
    13021 cgaagaatca aaagggatcg aaacagaact agatgttgta gaaggtatgc agtttgatcg
    13081 tggttactta tcacaatata tggtaacaga taacgacaaa atggaagctg ttctagaaaa
    13141 cccatatatc ttgatcacag acaaaaaaat ctctaacatc caagatattc tgccattgtt
    13201 agaacaaatc ttgcaacaat cacgtccatt gttgattatt gctgacgatg tagatggtga
    13261 agcattgcca actcttgtat tgaacaaaat ccgtggaaca ttcaacgtag tagctgtaaa
    13321 agctcctggt ttcggcgatc gtcgtaaagc aatgcttgaa gatatcgcta ttttgacagg
    13381 tggtacagta attactgacg atctaggttt agaattgaaa gatgcaacaa tcgaaaactt
    13441 aggaaatgct tctaaagttg ttgtagataa agacaataca acgatcgtag aaggttctgg
    13501 agaaaaagaa gcaatcgaag ctcgtgtgca attgatcaaa aaccaaattg ctgaaacaac
    13561 ttctgatttt gaccgcgaaa aattacaaga acgcttagct aaattagcag gtggtgtagc
    13621 tgttgttaaa gttggtgcag ctactgaaac agaattgaaa gaattaaaat tacgcattga
    13681 agatgcattg aatgctacac gtgccgcagt agaagaagga atggtctcag gtggtggtac
    13741 agctctagtg aacgttatca gcaaagtttc tgctgtagaa gcagaaggag acgtggcaac
    13801 aggtatcaag atcgttgttc gtgctttaga agaaccaatt cgtcaaatcg ctgaaaacgc
    13861 tggttatgaa ggatcagtga ttgttgataa attgaagaac gtggaactag gcactggttt
    13921 caatgctgct actggagaat gggtaaacat ggttgaagca ggtatcgtag acccaactaa
    13981 agtaacacgt tctgccttgc aaaatgccgc ttccgtttct gccttgttat taacgacaga
    14041 agcagtcgtt gcagataaac cagaaccagc agcgccagca gctcctgcaa tggatccatc
    14101 aatgatgggc ggtatgatgt agtaattaag aaagaaccct attttaacag ggttctttct
    14161 tctttttttg ttttatttta agcaaaaggg gcaaagaagg ggcaaaaata tcaaaggcta
    14221 tttagtttat caattacttg ttgtttggct ctttttgtta catggttata aatagacaga
    14281 gttgtgtttg catcagaatg ccctacacgc tccataatgg ctttaagagg tacgcctaat
    14341 tcagataata gtgaaacgtg gctatgtctg aatatatgtg aggatagact tttttctaat
    14401 tccaactctt cctctacttt atggagtata gcgttaaatg agtgcagcgc aagcggagtg
    14461 ccacttgtag atataaatat atattgatcg ggatctgtgg gtctacctgc aagaatattg
    14521 tcagctatca cactttcaat caattctttt gcacgattgg gtaattgcac ttcacgttgc
    14581 gaataagtat tttttggagt tgtttttata gcattatcca ttttcacaga tgtgtaatct
    14641 aaggtcccat taatggaaat tttcccatcc tcatagtcct tcatttgcaa agctagcaat
    14701 tccccatatc tcaaaccagt taaatataga aactcagcta ttatgccgtg tagttttctg
    14761 cgaggattgg agtatagctg tttcagtatt tgatcaattt cttctttctc cagatatttt
    14821 ttattcatag aaagccttct tttttcttct tccacttttt taggatggat tttaactgct
    14881 agcgcagggt ttctttgaat gtattttcta tcgattgcat agtttagcat aacagataga
    14941 gttgtttttg tttgttttgt gtagttcagt gagaggtcac caaacgtata catatcttca
    15001 attatcttat taatgagtgt ctcatcaatg tttctaacga tcgtatcatc gcttatgtgc
    15061 ttagaaacat gtttcatcat cattggaacc ttcaaatagc tagtacgttt aacatgctgc
    15121 ttataatatt cataccattc tttatacagc tcaccaaaag tgatatctga tttattgtaa
    15181 tcttcgagtg cttctttaat ttttttatct agaattttct gagctttttt ccacgcttgt
    15241 ggtgaattac ttgtaagtgt tgtagatttt tttcgtgttt tttctgtata aggatctaca
    15301 tatctttcaa taaacttgaa ttggccatct ttggtctgtt caacccacac ttttaacatc
    15361 tcctatcatt tgctataata ggcataacaa atagacctat ataggtttgt tttctaaaag
    15421 cacgctctta ctctggacgg tggggcgtgt tttttattta ttatacagtt gctttaaaaa
    15481 tagctgcttt ttcaaatgat actaaaggag agaagtggat ttctatttct cccttttggt
    15541 ttaacgtgaa atgagcagtt acatccattt gttttcctgg agcaacagat cccattgtat
    15601 tttcgttggc atatgtctct gattttttgt catctggtcc atatacttcc acatctgtac
    15661 ctacagggat atctgaatcg ccatcatttt ttacggtata agtaattttt actacttgtg
    15721 caggttggtt ttcttcaaat tgatttcttt catcagttag ttctacacta tttagtgtat
    15781 attcagcatc accaactttt acagtgtcac caatcttata gaaggtatcg cttttttctt
    15841 ctttagaaga tgaagcagag gttgaagatt tagttacttt ttcgccacca ttatcacttg
    15901 ctttattact atctgatcca ccatttaaag cagaaccaat aattataatt aaaattactg
    15961 ctaataccca aaaccatact tttttgtaaa aaggtttact aactttatac atttttccgt
    16021 cttgacccat aacttttttt gccatttaaa tattcctcac ttcttgttat aatatatttg
    16081 cgatctcaga aatgaggtat gagtccgtgt gccagcacgg gcttttttaa tgttttgctg
    16141 aaatcggttt ttttaataat tcttgatgta ttcatacata ttgccttgcg taagaatatt
    16201 ttttcttaaa atagcattgg cagacagcat aacaagtgta tctgagctta ttctgaatag
    16261 aataatactc cataaatttt tcgagattaa actgagattc atcagtcagt tcgttctcaa
    16321 tatagatatt taataaaatt agaatagcta ttttatctgc ttctgtttca aattttgagt
    16381 gaaaagttgt agaagtatcg tacaaaactg aatattcaaa atgagaagcg gtgaaatgcg
    16441 caagttcatg agataaatga aaggcttctg cagtttcgcc atatagattt tcattcaaaa
    16501 aaatgattct aggcttcgga taatagaagc caggctcttt catttccatg taaactactt
    16561 tcaaattgta ttcgctcaac atttctttca atttcaaata catacaaccc atcactccaa
    16621 ctattcattt tcctctaaag ctttagcaat tgcaatcgct ttacgcattg tctccttaga
    16681 tatttctttt ccatcaaaag aaaaaacagt atcgttttct gataaatcca catgtttagg
    16741 ggtttctctt tcttctctac ctagaaggta gtctacagag actccaaagt aatcagctaa
    16801 ccgagctaac tcaatagaat taggagtttg tcttttccaa cttcctaaat atccattcga
    16861 atacccgaaa ttcatttcga gctgtctaat ggataatcct ttttcttttg ctaactcctt
    16921 tattatttcg taagtattca ttgataaatc aacctttctg aatgcttaca aaaaaagttt
    16981 agataaataa tgtaaaaata gttgacttat atacattatt aatctatact atgttttgta
    17041 aacaagttta acaactaaaa agacaacaaa aaatactatt gattaataaa tgccaaccgc
    17101 caagaaagct ttttaaatca aagtttatat gtcttattta actatgcatt tagtatagat
    17161 tattaatcta ataaagtcaa cgcagttttt taaaaagttg ttaaatttgt ttacgaatat
    17221 aaaagaaagg agagaagaat atggaaaaaa cagtctcggc aaaaatcgaa gatttgaaat
    17281 tagacatact gaaacaagca aaagtggcga tggaacacgc ggtagataaa gaagactctg
    17341 ccatggttgc agccatagca gagattttag ctcacgttta gtcatcgttt ttacggtctt
    17401 ctggaagctg accatagata acagcataat gaatatctaa ataagcttga acaatttctt
    17461 ttggcgaaat tgattcgcct ttagtagata cttgtgattc gtgatttagc catgcaacaa
    17521 ctaaatccgc agcgatttca aacggcgtta cactttgtgt cagttgccat aaagaatttc
    17581 acaatatgta tggtcgtggt aataatacca aagagcaatt tgaggaattt atggaaatta
    17641 aaaaatacaa aaaatcattt attaaaggag gctaaacaat gaacaaggaa atgagccaaa
    17701 caatcaaggt acaaaaaatg atagatgatc taacacatgg aattgatagc caagccgata
    17761 aaatcatcaa ggaattgcaa ggacaaaaag ttaaggatgc aaaaatgctt ctcaagacta
    17821 tcaactttga aatgaatcca acaaaaagaa agctcgccga tgtgttggaa gaaaagttgg
    17881 cttccgctat caacgagcaa gaattactat ttgaaaccga tacttttaac agctgatgtt
    17941 ttatagatga cagtttcatc cgaatctata tcatcgccga gagaaaagaa tgttgctttt
    18001 gacaaaaagc cgataagacc tacaagcggt cttgatgttg caagagtaga taccccaata
    18061 tcaccttgca aggtattcac gtaaatttga gaagaataga acattccatc tttttcttcg
    18121 atatttgacg catcatcgta tccaacgacc aaagtatcgg caaatatttt gattttttct
    18181 ccatcaagca tagtaattac aaattctggt ttcataaatt ttcacctcct taacaattat
    18241 ttcagcctgt cacactgata aggaaattat accaaagaaa ggaatgagaa atatgaatac
    18301 accacaaatt tttaatttcg agcaaaacga agttcgaact attttagtaa atgatgaacc
    18361 atattttgta ggaaaagatg tagcagacgt tctaggatat tcgaatccgc aaaaagctat
    18421 tcgagatcat gtggacttgg aggataagac gcagaacgat tcgttcaccg tcaatggaac
    18481 agcagttgtt ttgattaacg agtcaggcct ttacagttta atcttaaaat caaaacttcc
    18541 ctctgccaaa aaatttaaac gttgggtaac gagtgaagtc cttccaacaa ttagaaaaac
    18601 aggtagctat tcaaacgtac ctcaaagttt tgcacaagca ttgcgtttag cagcggattt
    18661 agaagaaaag aaccaattac tcgaacaaca aattgccgag tacgaaccaa agattagcta
    18721 cttagatacg attctttcat cgacagatac ggtagctact tctcaaattg cagctgatta
    18781 cggaatgtcg gcaattgctc taaacaaatt gctaaacgag ttaggtgttc agcataaagt
    18841 tagcggacaa tggatacttt accgaaaaca tatgaaccaa ggatacacaa aatcgcacac
    18901 aagtgagata ccgaaagccg atggcggcac taaagttgta atgaatacca aatggacaca
    18961 gaaagggcga gtgtttattt acaacttatt aatcgcagag ggctattacc ctcaaatgga
    19021 tttagaggaa attggttaga aaggagtttt agtatgactg acattgcaga aatcactcaa
    19081 cgagatagag aaaaaatcaa agaatatgtc gaaagttcaa agttcttaac ttacaccatg
    19141 cttgctgaaa gatttggaat tagcaaaagc tacttatctt taattttaaa cggtaaaaag
    19201 acttctgcag aagcaaacag aattatagat tcgattatca ctatgtacga attgtagagg
    19261 gaggtaaagt cgttggaaga taaaatcatt cttacaaaat cagagcttca agaaatgtta
    19321 aacatcgagt atggaacgat aatttttagg agggataata agtgaaaaaa ccaacacttt
    19381 cagagctgat agaagctgct gagaaggcag taaaaccaga cgactggtac cgacaaagtt
    19441 taatcttgga gaagttccac ggcatgtcaa aaactacttt agttgaatac tgcaaggaaa
    19501 tggaaacaat tcctgaattt gcagaaggaa tacttcgtcc aggtcactca accacattta
    19561 ttcattacca tacttttatt tggtttttaa agtggaaaga cgcaaacaaa tatcgtgtaa
    19621 aaagtttatc gccttcagat gttttgaagg aggcaagtta atgggcaaat tcaacagagc
    19681 actagtattc agcgcaccgc taatcatcta cgctttaggc ctctggggaa gcagacaagc
    19741 gttgataggg acaatcgttt acatggtttg gatttttatg gggcttgatg aagctgagca
    19801 cagagcgaaa aaagccagtc gggagggact gactaaaata tgattttttt atttttgttc
    19861 ctttgggctg ctattgcgta cttcatccag ctccttgttg tcaaagttgc tcttatttct
    19921 tttaggaaga acaaaagaaa atagcgctat aaatgaaaga taagtagccg ctacattaaa
    19981 ctgttctttt gaaaaagtta cagtcatcgg aatgataatt gatattgagg aagtaatctt
    20041 aattaaaaat atagcaatat tttcaacatc gcgttggttt ttatttaaca ttgtccaaca
    20101 tgttttagca acaataaaaa taattacaca tgatattagc acacagactg aaaaaagttt
    20161 tagaaatgcg atgtagtcag tgttattgaa ctccttcaaa atactatttt tattcaatgt
    20221 aataaaagga atttttttaa ttgaagacag gaaagataag gtaataaaca gaattatata
    20281 tatggttaaa gtcgcagtaa gaatgtaatt gagctttttc ctcatagaat cacctcactt
    20341 tctaacgaaa attataccaa aaaggagaga agaaataatg caagaattag taattttgaa
    20401 aaataaagaa gctgtaacta cgagcttaca agtggcagaa agtttcaaaa agaaacataa
    20461 gcatgtacta gaagcaattg aatcaataaa aagatcggtc gaaaattcgg ccaatgttga
    20521 agatgggtcc aattttggac agatgtttgt ggaagggaac gagccagact catacggaag
    20581 aagtcggaga gtttatttca tgaatagaga cggattttcc ttgctagcta tgggattcac
    20641 tggaagtaaa gcaataaatt tcaaactaaa atttattgaa gctttcaacg aaatggaaga
    20701 tgttattcgg aagaatactg ttcctcaaac aattgaagac atgatgatct atcaattaga
    20761 agaaatgaaa gatgttaaaa aagatgtttc catgcttaaa gatactatgc gaattagcgg
    20821 acaacaagag tttgaaatta agcaaaaagg aaatatgaaa gttatggaag ttctaggggg
    20881 aaaagaaagc cgagcttatg aagaaatcag caaaaaagta ttctcaaaat tttggtctga
    20941 atttaaacgt accttttcaa tcccaagata tggcgagtta cctcgtaaga gattcgatga
    21001 tgctgtttca tttattgaaa tgtggttacc agaaactgcg atccgcatgg aaatcgatca
    21061 actgaacaga caacaaagac ttttcggtga tgaaaatgaa tagagctgaa gcgctaagaa
    21121 tagggacggt aattgctaat cgctggtgga gacacaataa accaaacatc ctaagccaac
    21181 aacatatcga aaagcaaaaa gcatggcaac aaataaaaaa gtgactcagc cggcaagcat
    21241 agagtcacaa aacatatcta aggagaattt tagcatatga ataaagaact ttccacttta
    21301 gatcaatatt tgactgattc tgaatggggc aagtcgaata tcaaggaaac aaataatcga
    21361 aaaatcagac gaaatctttt gacgaacgaa gaactatcat gtgatcaaga tgacttggga
    21421 aattttgtga gtatttggga tcatgtttac cttatccatc tatcaaaaca ttcaaataaa
    21481 cctgaataca tctatgtcat cgaagatggc ttgattgatg cgctagaaga gtacaacaga
    21541 gataacttga ttgatatctc ttattacgga tcaggtaaga aatacattgc tgaaatggag
    21601 gcagaatttg atgagtgaaa tcaaagggac aacgaacttt gaaaaacttt ttagtcgtaa
    21661 gttaaataaa attctcaaga aaaaaggaaa ttttgattat ttatcttggg ctcacgcgtg
    21721 ggagattatg aaaaagaatg atccacaggc aacggtaact attaatgagt ataaacacta
    21781 cagggttgtt tctggaactc atcaagactt tcttgttgag gaatataaac cttttcttat
    21841 ggatgaaaca gggacttatg tatctgtctc agtaacggtt aaaggacata cggaaacaga
//
LOCUS       DAYYCY010000031        21740 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000031 DAYYCY010000000
VERSION     DAYYCY010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.031"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(205..1104)
                     /locus_tag="ACHAZB_000957"
     CDS             complement(205..1104)
                     /locus_tag="ACHAZB_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM8613648.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            complement(1114..1470)
                     /locus_tag="ACHAZB_000958"
     CDS             complement(1114..1470)
                     /locus_tag="ACHAZB_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFM8613649.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            1825..2121
                     /locus_tag="ACHAZB_000959"
     CDS             1825..2121
                     /locus_tag="ACHAZB_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HFM8613650.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            complement(2765..3718)
                     /locus_tag="ACHAZB_000960"
     CDS             complement(2765..3718)
                     /locus_tag="ACHAZB_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8613651.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(3741..4496)
                     /locus_tag="ACHAZB_000961"
     CDS             complement(3741..4496)
                     /locus_tag="ACHAZB_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8613652.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            complement(4493..5452)
                     /locus_tag="ACHAZB_000962"
     CDS             complement(4493..5452)
                     /locus_tag="ACHAZB_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFM8613653.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            complement(5445..6395)
                     /locus_tag="ACHAZB_000963"
     CDS             complement(5445..6395)
                     /locus_tag="ACHAZB_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFM8613654.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            6545..6997
                     /locus_tag="ACHAZB_000964"
     CDS             6545..6997
                     /locus_tag="ACHAZB_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HFM8613655.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            complement(7086..9353)
                     /gene="gshAB"
                     /locus_tag="ACHAZB_000965"
     CDS             complement(7086..9353)
                     /gene="gshAB"
                     /locus_tag="ACHAZB_000965"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HFM8613656.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(9540..10538)
                     /gene="ftsY"
                     /locus_tag="ACHAZB_000966"
     CDS             complement(9540..10538)
                     /gene="ftsY"
                     /locus_tag="ACHAZB_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFM8613657.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            complement(10549..11358)
                     /locus_tag="ACHAZB_000967"
     CDS             complement(10549..11358)
                     /locus_tag="ACHAZB_000967"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8613658.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            complement(11367..14948)
                     /gene="smc"
                     /locus_tag="ACHAZB_000968"
     CDS             complement(11367..14948)
                     /gene="smc"
                     /locus_tag="ACHAZB_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HFM8613659.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            complement(14968..15654)
                     /gene="rnc"
                     /locus_tag="ACHAZB_000969"
     CDS             complement(14968..15654)
                     /gene="rnc"
                     /locus_tag="ACHAZB_000969"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFM8613660.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            complement(16002..17783)
                     /locus_tag="ACHAZB_000970"
     CDS             complement(16002..17783)
                     /locus_tag="ACHAZB_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8613661.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            complement(17809..18723)
                     /locus_tag="ACHAZB_000971"
     CDS             complement(17809..18723)
                     /locus_tag="ACHAZB_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8613662.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            complement(18750..19712)
                     /gene="opp4B"
                     /locus_tag="ACHAZB_000972"
     CDS             complement(18750..19712)
                     /gene="opp4B"
                     /locus_tag="ACHAZB_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFM8613663.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            complement(19712..20656)
                     /locus_tag="ACHAZB_000973"
     CDS             complement(19712..20656)
                     /locus_tag="ACHAZB_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8613664.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            complement(20657..21661)
                     /locus_tag="ACHAZB_000974"
     CDS             complement(20657..21661)
                     /locus_tag="ACHAZB_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8613665.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
BASE COUNT         6075 a         4639 c         3847 g         7179 t
ORIGIN      
        1 tatatttttt tttgtgatta tcgttcaaaa aaagaataca cgcttgcatc tagtactctg
       61 cgagttacaa atcatccgat caaataaaca ttcgctgatt attttccgca aaaaaagcag
      121 catatccacg ataaatggat ctactgctct tacttatcct tatgttttac aaggtatttt
      181 cttttacaca ttactaatgc tgtctcaata cggaacgatt gtcatcgctt gttcaaattc
      241 ctcttgcaaa tgttgtgttt gataggcctg aaaagctggg tgatgggcac gcaagaggac
      301 acgtaatttc ataggatcat agctgtctag caactgttct ttcaagacaa aatctcgggt
      361 cagctgccat tcataataat aaccaaaagg tctgcagctg taaatcgtgt gaccgatgga
      421 tttatcttca aagcgacgat gcgtatggcc aaaaactact tgtttgatat ttgaataacg
      481 gtcaagtaat tctccgaatg attctgatcc taaaaatgca tttaatttgt tccaacgctc
      541 ataaggagca gattgataca tgataaatgc ccgtttagga acgaaatgag tggataaaat
      601 cacctcaagt ttttttgctt ccagctggtc tagcaaattt tttgcagctg caagcatttc
      661 tgcattgacc tcgagatctg ttttcccacg ctggatgaac cgatcatacc aatacaaacg
      721 cttcattcgt acgattgcct cgtaatcttt caattcggaa aactgatagt cgtaccagcc
      781 attcatccct aaaagaacgg tctgctcatt taaagcaata taccgttcat ttaaaaaatg
      841 tgggtctgga aaatggtcca tcatctgttc attgggcaca tctgccaact cgtgattgcc
      901 gaaatgaaat gtcgtagata atcctttatt gacgaaaaat tcgacaacag ctaggcaacg
      961 gtctacttta ttagccgtat ctccggctag atgaagccga gtgatcttgt gcttttgcaa
     1021 aacttgccaa agctgcatca gctctgattc gccgaattta ttgatgtcca catgtaggtc
     1081 gctgatgatc gccaattttc ccattttatc cctctagtct tttcgaacaa cttgcaagtt
     1141 gccttttctc aagccgcttg gtccttttaa ttgtaaacta ttataagttg gttctaaagc
     1201 aagtgtggat ggatcatcca tgtaaagtat tgcactgtct ttgattttga ttggtccaac
     1261 tggtgtatca gccactacag aataaagact tagatctgtt tgtgtatctt ttttgacaat
     1321 gatcaatcga aatgccgtat ccaattggca actgacttgg ctttctgcaa atggaccgtc
     1381 accgttttcg atatctaata aaagttgatc gtctgcttct aatttattct tcaatacttc
     1441 ttgtgcttct tttgtcactt ctaatttcat ctgtatcacc tcatgtcata gaataatcac
     1501 tttattcatc agatgcaact aatttgtctg gttctgactg ttctcaattt attggaaagt
     1561 aagtcattcg tttattatga tttctcttat cacaaaagag acaactttcc cttttcttcg
     1621 ttcagtttct tgaacggaaa aagacccaaa aagcaacctg ttatttgttt aaccttattc
     1681 ctttcttaaa taacaaaaaa tactataaaa aataaaaaac ctttaacaac tattggaaaa
     1741 agaattactt tttgttcacg gattattaac aactcttcgg tagagtaaac ttatcaaata
     1801 aaagacttct ttggaggaat cactatgaag gaatatataa aaaacatcta ttttatcgaa
     1861 gaaacacaaa atatcgaggg ttcttatatt gaagtaaaaa cactctttgt caatgaagat
     1921 aaaaccaaag cattggatat ttataaaaaa ttagcttcaa aaaaaacgaa tagttttgga
     1981 ttgatattaa gcgagtataa aatcaaagca gaagagagtt atttctatca attattaaaa
     2041 cggtggagta agcttcccgc cgatttttat cgaaaaatgc agatcatcaa ctatcaaccg
     2101 cttgctgaga cacatgccta aatagaggtt gtgaaatcag tgctttgagc taataccgaa
     2161 ggtcagctga ttgaacattg tttagaagag attgtgacaa ctgcgatttg ctttgagtaa
     2221 taagaacgct ggacagaaaa tagcttctca tattttctag tctagtgggc ttattgccga
     2281 gaagcagcag atggaacatc gtttagaaga gattgtgaca actgcgattt gctttgagta
     2341 ataagaacgc tggacagaaa atagcttctc atattttcta gtctagtgga cttattgccg
     2401 agaagcagca gatggaacat cgtttaaaga agagatcggt acaaaataaa aatatctaaa
     2461 tttaagtttt atctaagttt cgctttgtac ctttgattca aatcaaagga ggcgatcttg
     2521 atgctgaaaa aatggatcac actatttttt gccactcccc attctagaat caacccgtat
     2581 gatgtagata atgaattcac gttgaaaaaa gatttctcaa ataaataaaa accatccgta
     2641 aaatcatcaa tacgatttta cggatggttt ttgataactt ctttgaggat aggctcttgc
     2701 tatttaagaa tgtaagaaac attattgtta catagtatga gagtttaatc ctctgttatt
     2761 tatgctattt aaatgcttga ttcacatctt cgatcatcag tttcattgat tctaaaccgc
     2821 ccccgcttaa ataccagaca tctggttcaa gggagatgat ctgctgattt ttaccagcat
     2881 tcgtttgtgc taccagttca ttggcagaaa tatcatcttt gctgtcgtct ccaccaatag
     2941 cttttgttct gtctactaca aataagatat ccggattttt ttccagcaca tattcataag
     3001 aaacactttg accgtgagtg gatgcttcga tttgatcatc tgcttgttca aagccaaata
     3061 aatcatggat gaaaccgaat ctagaaccag atccgtaggc tgataattgc ccttcattca
     3121 caagtgtgac tagtgctttt ttgtcagtag cttctgcttt ctttttcgtc tgatcgattg
     3181 cactagataa ttcctctaat tgagttttag cttcttcttg cttaccaaag atctccccta
     3241 aagcagtgac attttgctgg aaagattccc atggattttt cgcatctaaa gaaaggaaaa
     3301 tggtcggagc aatttgactt agatcttttt gaaaatctct ttgtcggcca gaaatgatga
     3361 ttagatctgg ctgaagctga ttaatttttt ctaagtctgg ttcttttatt ccacctgctg
     3421 attctacttt tttataggat gaaagatact caggaaggct gcttgtagct gctccaacta
     3481 ccttatctcc aacaccaaga gcatccatcg tatctaaaga accattgtcg aatacgacga
     3541 ctttttctgg attatttggc acctcgattt ggtctccatt actatctgtt atcgtcaatg
     3601 tagtttcagt ttgtgtgctg cttttttttg tgtctgcctg actggtgttg ttgctatttc
     3661 cacatgctgc taaaccaaat gatgctagaa tcaagatacc tactactaat tttttcatct
     3721 cttctctcct aatgttagtt ttagaaataa agacagaatc tgcggccttc aatctcacaa
     3781 atccgtatgt ccatttcgta taattcatcc aatacatctt tttttatcat ttcttgggtc
     3841 gttccttgcg taaataactg tccatctttc attgcaacaa tttcgtctgc ataacttgca
     3901 gcaaagttga tatcatgaag aacaataaca attgttttcc cgatttcttg tactaaattt
     3961 tttaaaatct gcatgatttg aaccgcaaaa ttcatatcta gattgttcaa aggttcatcc
     4021 agtaaaatat aatccgtgtc ttgcgctagc accattgcaa tatacgcacg ttggagttga
     4081 ccgcctgaaa gagtgtcaat cgattgatcc gctatttctt ctaacccaag gtgttccagt
     4141 gattcctgaa cgatttcctc gtcttttgtc gtcaattttc ctttcgtata aggaaatcgt
     4201 ccaaattcta ctaattcttt cacggtgatt tggacattta tttcattcga ttgtcgtagg
     4261 atcgataatt ttttggctaa ttcattggat ttccaagcct ttacttcgtt atggtctaaa
     4321 tagatttcac ctgtatcttt tggtatcaaa cgactgatca tcgacaacac cgttgacttt
     4381 ccagcaccat ttggaccgat aaaagcagtc aatttctttt caggaatcgt taactgtaca
     4441 tctttaacca ctgattttcc atggtacagt ttattgacct tttgtaaatc aatcatagga
     4501 tactcttcct ttcgcgaatc aattttgtga caaaataaat tcctccgaca aattcaacga
     4561 cgacactcac tgttgtattc cagccgaaaa tctgctctac tgcgagctgt cctcctgtca
     4621 gaaacagcat agctaccaga aagcttccga taaatagctg accatgacga taagtagaaa
     4681 agacacgata actgatattt gcgacaataa accctaaaaa agtaactgga cccacaagag
     4741 ccgttgatgt ccctgtcaaa agactgacac aacataaaat caaaaattca aaacgatctg
     4801 tgtctatccc gaggtttact gcttgttcat ttccgagatg gaaaacgtct agcacatgtc
     4861 gatatcgaaa taacaggtac cagcagccga taatgatcaa agaagcaggc aaaagatact
     4921 gactctggat attgccaaag cttgcaaata atctgccttg aagcaaatca tattcattcg
     4981 gatccatgat gacttgtaaa aaggtactga cactaccaaa aagcgtacct aaaatcatcc
     5041 ctgacatcag taataaaaaa agattcgctc tcattttttt caaaagaaag aaaaataaca
     5101 atagactcag cccgaccatc agcccaacgt tcaataagaa aaaaactgta cctgtctgct
     5161 gaagcatcgt cactcctccg ataaagaaga aaaggaccgt ttggacaagg acatataacg
     5221 actcaagtcc caatactgaa ggagtcaaaa actggttatg cgtaattgtc tgaaagctga
     5281 ttgtggcact gctgacagct agcgagacaa ggataaatgc aaaaattttc ttactcctca
     5341 atgcacatgc aaaatgccag tttccatatg tatggatcgt caagtacatt cctgtaacaa
     5401 ataaaacaag aacaagcata ccaataaaaa tagatggctt ttttttaagc atatcgtcgt
     5461 ccccttatca gaagataaag gaagatagcc ccaccgatga ttccaacgat cacactgact
     5521 ggtatttcat aaggaaaaat caacacgcga gcgacaagat cgcaaaatac taagaacaaa
     5581 ctacctgcta aagcagttac gccaattgtt ttgtgcactt gatctccata tattctagag
     5641 acaagattcg gaatgatcac tccgataaaa ggaatgcttc ctaccatgat caacatgact
     5701 gcactagcca atgatactaa gccaatgcct aaaaactgca tcttctggta atctattccc
     5761 agatttactg caaacgattc gcccagacca atgatcgtaa atcggtaagc aaatagccaa
     5821 accaagagca acaatggaac aacagcatac aataactcat aatttcctcg tatcacagtc
     5881 gcaaaattcc cttgaagcca tgaagtcata ttttgtacca actggaattg ataagcaaaa
     5941 aaagtagcaa ctgaaccaat gatattccct aacatgactc caatcaacgg aagcatcaat
     6001 tgatttttct ggaggaacaa tcgcgtaagg gataaaaaaa gcaatgttcc tgcaaaagca
     6061 aagcaaaatg ctgtcaagga tcggacaatc aaggatgcac caggtaggaa aatcatagcc
     6121 actaaaatac ctaaacgcgc actatccatc gtgcccgcag tcgttggtga aacaaattta
     6181 ttttgggtca aatgttgcat gatcaaccca caaatactac tggtcgctcc cacaaggatc
     6241 aaacaaatcg ttcgtggcaa acgagtcact agcaagattt gtaagctttg cccatctaac
     6301 tggagcagtt ctgacaattg tacattcgat actccgatga acaatgaaat actacttaaa
     6361 ataactaata atgctactag tcgtccgtag cgcatccaat ccacctttct aattgagaat
     6421 gattttcatt cttattttaa gtgaatcgtt gaacaaaagc aaccgctttt tcatatgttt
     6481 taggtattat tgggaatatg ctataatcgt aagaaagaca aagagaagaa ggagggtatt
     6541 ttttatggca caagatgatg taaaaagcca tttggataag ggactttatg gtacaccttt
     6601 agtgaaccct gaagaacagc ataaatattt agggacattc agggaacgct gctatcttag
     6661 tatgactgtt gctgaaatga tccaaccaaa aaacaaagaa aactttttaa aagaactcaa
     6721 aaaacatcca gatgcttcca tactcttgaa tggggctcta cccctttctg tccaaaacca
     6781 atatatccag ctagcaacga aaaacaatag ccgtttcact gtagtcaacg attacgtcac
     6841 tgcaaatcct gaggaattcg gattgctctt aacagcaaaa gaagcagtaa atgaacctgt
     6901 gattgatatc gaaaaaaaat acccagaaga aaactcttcg ccagctgaaa aagaaaaacc
     6961 aaaaaagaaa aaaagcttct gggatcgttt attttaaaac gaaaaaacac ataaattttt
     7021 acatgcaaaa aaagcttttg atcagattga catcaaatag gacaatctgt caaaagcttt
     7081 ttcttttaaa atacttcagg atataacaat ttcaatacat tcatcgtcaa tcttctgcct
     7141 tttcctgcaa aaggataaac atgcatatgc atggctggat tgaaatttgc ttcgatgatc
     7201 ccatatgctt ttttatcagt tgtaggatcg atttctttat ctggtataat caaatcgata
     7261 ccactaatct tagctcctag tgcttctacc gcagaaacgg cgattttttt gtaagcttca
     7321 gaaaattcat cagtcatatc gatcgaatca ccacctgtac tgatatttga gttctccctt
     7381 agataaacaa tttgattggc ttggggaact gattcgatcg tcaacccttg ttctttcaac
     7441 atcaattgtt ctaattcacc taattggatc aattctaaag gtgcacgatg attcgttcca
     7501 cgcaaagggt cactattttt ttcttcaaca agttctttca ctgttcggat actgtcacca
     7561 ataacattag cagggactcg caacatgatg gcttggactt ctccgtcgat cacaaagaaa
     7621 cgatattccg ttcccggcat aaattcttct actaaaaccg aactgtcttc acgaaaagca
     7681 atggctaaac ctgcttgata atcttccaga ctagctcctt ccttgaaaat cgtgatacct
     7741 aacccgtaat tggttgattt aggtttgatc acaaatgcct gctcggcaaa tctcggataa
     7801 gcttttaccg cttcttccat ggatgaaaat tctgcgcctc ctggtacccg gaacccagct
     7861 tcttttaaga cttttttcgt tactgttttg ttttccatga taagtgggac gatatagctg
     7921 tccttacttg tcatgtttgc atttttcaca tattctacat ggtcttggaa ttgcagacgc
     7981 aaaaattgat ccgactcatc taatactttg acttggacac ctttttggat ggcatcgaac
     8041 ataaatatct gagtagaaag ttccatttcg cgaaaaccgg ccaactgata gggacgttca
     8101 tggctttctt tataatattg atgtcccaat gccacagcaa cttcttgctg actcgatagc
     8161 tgtgcttctt gatagagttt cgctgctaac gtttgttcag gatgatccat ttgagtcaat
     8221 gcttcctcta ctaaataaag agaatccaac caatccaatt caaccagcat ttgcttcatt
     8281 ccttcaagaa tttctctacc ttcttgatga aattctgttt gatctgatgg ttgttctagt
     8341 gctactttgt tatttcgagt cgtaccttct gctacccatt ggtcacagtc ttctttttct
     8401 tctgtccata acatgtacat caggaataat tgtaagaacc gaacttgctc tggattgata
     8461 cccaatcttg cataaggatt caaatcgata ttccgcaatt caatataacg aacacctgtt
     8521 gttgctaaat ctgcgacttt ctttccgcct cggaaacgaa gcggtgtata aaattctttc
     8581 tcctccgaaa gtttcccttc ttcgatcatt cgctcgatat ctgctaaata ctgtttcata
     8641 gaagcatagg aaactttcac atttggatga ttcgtatagc ccagcgcact gttgcgaata
     8701 ctgcgaacag gttcttgcgg atgtgattca tctaaaaagt agttcttctc actcattggt
     8761 gaagcaccga ataaataagt gatcatccag cgatagcgca taaagtttct cgctgttttc
     8821 aaataaagtt ctgttttgaa ttcggaaaaa tcttgaaatt cttcctgatg gctgaacaat
     8881 gttcttagca actcatcgcc aaattcaaaa ttgaaatgaa taccgcttac catctgtttt
     8941 cgcttcccgt attcttttgc taaataacgt cgatacaaga catcttcgaa attttttaat
     9001 tttgcaataa tgatctcttc gtctttttcc ggtaaggcag gtggcatact taatggccag
     9061 atcatctctt ctttcggtat agaacgagca gtcacatcat aaacagcagc gagatactga
     9121 aataattcgg gaatagaatc ggtaacaggg gtgatcatct ctgtttgtgt ttcgctaaaa
     9181 tctgtttgga tataggggtg ataggatcga tcgccaaaga ttgctggatg atcggttttt
     9241 gctagatttc ctgcaagatc aacccgctgt ccttcccgtt ctataccaaa tcgagcttgg
     9301 tcgataaaag ggcgcgcatt gacatgcaat aataattgct taaaattcat cattaagtga
     9361 cctccaattc atatgaatct aattataatc aaaaagtcta agaaaaagaa cgaaattgtt
     9421 tgagaaccgc ttacaaataa gtataaaaat gaaggcatca aaaacacagc tatgttaaaa
     9481 acaatcattc aaacaaggag ctgcaacttt tgtcacagct cctgcttttt agacatgggt
     9541 tattcttctt ttagtaatcc tttgaacaat ccaacgacaa aatcatttgg atcaaacggc
     9601 tccaagtcat cgattccttc accaagaccg accaatttca ctggtaaatg caattcatta
     9661 cggatagcaa gtacgatacc gccctttgct gtaccatcta attttgtcaa aacaagaccg
     9721 gtcacatctg ttgtttcttt gaattgtttt gcttgaacca tcgcgttttg accagtggtt
     9781 gcatctaata caagaagtgt ttcttggggt gcttctggta attcccgctg gattactcgt
     9841 tttatttttt ctaattcgtt catcaagttc actttatttt gcagtcggcc tgcagtatca
     9901 actagtaaaa catctgcctg ctctgtcttt gctctttcca aagcatcgaa tacgacagct
     9961 gctggatcgc ctcctgcatt gccacgaaca acttcaacac cagcgcgttc gccccagaca
    10021 actaactggt cgattgctcc agcgcggaag gtatctgctg ctgccatcaa tactttttta
    10081 ccttcgtttt tgaactgatg ggcaagttta ccaatacttg ttgttttccc tacaccatta
    10141 acaccgacaa acaaaataac tgttagtccg tcttcttgga tatttagttc atttacttca
    10201 ttgattcctt ccgcttcata gagatccaca agtttctcga tgatcgcatt ttggacttct
    10261 gccgattttt tcgcatttct gagtttcact tcttgacgta atgcttcggt aattcgtatc
    10321 gctgtttcaa aacctacatc tgcaccgata agtgtttctt ctaattcttc aaaaaagtct
    10381 tcatcgactg ttcgaaaatt ggcaaacaac tcattcatcc gttcaccaaa tgttttacgc
    10441 gtcttttcta aacccttgtc gtacttctcc tgtacttcaa cttcttctgt tttctcttcg
    10501 ccagtaaaag cacgcttgat tttatcaaaa aatcccattt gactccccct actctaatac
    10561 aaatttttgg atcactttgg ctacgccatt ttcttcattg ctggctgtca cgaaatctgc
    10621 tgcttctttg acgaaaggca cagcattctc catcgctacg cctagtccag catattggat
    10681 catcggtaaa tcattttcct catcaccgat cgccatgatt tcttcttgtt tcaaaccaag
    10741 atcttttgcc aataaagaaa taccataagc ttttgtaaca ccttttggca taaattccaa
    10801 aagcatactt cttgttttga tgatctcata catttcatgg aattctgctg gtatttcttt
    10861 gattttagga tctaattctt cttggtcata agcgattact gccttattaa gcacaaattc
    10921 atctgtaata tcgcttatat ccgctggttg gaattgcagc agcttattca acacattgta
    10981 caaggattgc ttccttgggg cactaggcag ctgtaaaacc acattgtcag acaaaacatc
    11041 tagtggcaga tccaattgct gagccagttt gtataagcgt tggatatctg atacttccat
    11101 cactttcttt tccatgattt caccagtatc atttttttgt accaaaccgc cgttgaaagt
    11161 gatgctgtag tcaccttcat ctaccagtcc taattcttgc agataatgcg ccatcgcagc
    11221 taaggggcgt cctgtacata gaacgatctt gaccccttgc tgttttgctt gtgctaatgc
    11281 ttgtttattc tcatctgaaa tcagcttttg ttcatttaat aatgtaccgt ccaaatcgat
    11341 tgctactaat ttgatcattt tcgttcctac actttcgttt cgttgatttt cgcctcttca
    11401 ttgatatctt ccatccgtac agaaacgatt tttgatacac cggattcctg catcgtcact
    11461 ccgtacagta catctgctgc gaccatcgtt cctttgcggt gtgtgactac gataaattgt
    11521 gtgtcatctt ggaaatcgct caagtaacga ccaaaacgtt tcacgttggc ttcatctaac
    11581 gctgcttcta cctcatctaa gatacaaaac ggaacaggac agacacggat aatggaaaag
    11641 agcaacgcga tggctgtaag tgctcgttct cctccagaaa gcaaacttag actttgcagt
    11701 tttttgccag gaggttgtac ttcaatctca atacctgttt tcagcaaatc agttggttcc
    11761 gtcagtacta attcagcttt accgccgcca aacatatttg gaaagacaat tttgaattct
    11821 tgacggatag cttcaaatac ttctttgaat cttgtccgca cttcatcatc catctcatcc
    11881 atcgtttcaa acaattggtt tttcgctgct aacagatcat ctcgctgtgt tgctaagaat
    11941 gtatgtcttt cactgacttg ttcgtattgt tcgattgaat ttaagtttac tggtcccagt
    12001 ttttcaatct gttcttttaa ggatgaaacc tttgttctac tgtcttcgat atcggttgtt
    12061 tcttgatagt cttgggaagc tttctcaaat gtcagctgat attctgactg cagataggtc
    12121 aaatgattat ctaaaatcat ttcagctcgg tctttttgga cttctaattt ggattgttcc
    12181 gacagtcgtg ctttttgctc acgattacgc tctgccaaga ctgcttcaag cttatcgatc
    12241 tccttttgcc ttctatctcg ctgctctttg gcttttgcca actcggcttt taatgtttct
    12301 cgctgagcag ataattcgtt aatttgtttt tccaaacttt cttctgtgac ttcatgatca
    12361 gaaaagtctg ctgtcaatgt cgccaactgc ttttctatcg cttcttgacg ttcggttgcc
    12421 tctgcttttt ggactcgggc accgcggagc tggatctgca gatgattaaa ctgctctttc
    12481 aagacagcaa gatctgcttg ttcttgtgct ttttgacttt gtacttgtgc tcttctggct
    12541 tccatctggt cgctttcttg acttaacgat ttgatttctt cgtcgatttt ttgtcgttgc
    12601 gattcaagat ctgtttgttt gtctttcaat tcgttcgttt gcttttcgta atcttcgata
    12661 aactgctgca gttcacgtgt ctcaaaagtt gagatttgct tttctttttc aaaacgttca
    12721 agttcattta tgatgttttg tagctgattt gtcgcttctt gttcttcaaa acgtaattgt
    12781 tcgcctcgtg tacgcaaaac ttcttgttct tcggctaaac gagctgtttc ttgttgcagc
    12841 tcctgaactt ttttttcttg tgcttggagt tgtttatctg cttcttcaaa ttcactagtc
    12901 aactgtttca attcttgatt ttgtacaaac aaactgcctt ggtttcctcg tttattcgct
    12961 ccacctgtca tggatcctcc ggcattcatc acatcacctt ctaaggaaac cactcgatat
    13021 tgatagcgga tcgtttgcgc gattgcattg gcactagtta agtctttggc taaaaggatc
    13081 gttcctagca ggttttgaac cacagtttgg atttgatcgg gataactgac ttgttcactg
    13141 gcaatcccta taaacccttc tacagctgct gcttgtgtca atatatgagc cggtaattgt
    13201 cttggtttga ttgtcgtcag tggaaggaat gttgctcttc ctcctctttg ctgtttcaaa
    13261 tacgtgatgg cttgccgagc atctttttca ttttcaacga tgacatgctg ggctgcccca
    13321 cctaaagctg tttcgatagc taacgtgaaa tctgcaggaa catcgatcag ttcagccact
    13381 gctccgacaa tacccgaaag ctgctgttta tgctgcaaga ccaatcgaac cccttggtaa
    13441 aaaccaaaat agttttcttg gatttcttga aggcttttct tacgagcacg tagctgctgg
    13501 acttgattca ttaattgata cattttaggc tgttcttttg ctaatgcttc ttggaacttt
    13561 ttcccgtttt gttggatcat ttctaacttc tcttttgttt cagttaaaga tgattttagc
    13621 tgcgcctgct tttgtgtcaa ttcttctttt tgtaagatca atcggtctac tgatgcttct
    13681 acttccgatt gcttggctaa cgtctgcttg ctttttgctg tttcttgtat atattgacgt
    13741 tctagatact tcagttcgtt tcctacagct gcttgttctt gcatcaaatc aacatattga
    13801 tctcttaatt ctgccaataa ttctttcgtt gatttttggt atttttctaa ttcttgctgt
    13861 gtttttatga gatttgcttc agctttttgg atctctgttt ctttttctgc tgctgctttc
    13921 atcaagcttt cttgaagctt ttcaaaatgt ttaacctttt tttgtgcttc tgctaatgaa
    13981 gtttgatatt cttgcgagct tttttgtgta tgctttgtcc gctcctgcaa aacatctttt
    14041 tgtccttctg tctgcttcag tttttccgaa agatctaata atacttgctg atttttttcg
    14101 atcagacgat cggcttgcgc attttcttta cgctgcttcg ccaaaatgga ttcttgttct
    14161 tggatcgact cagatagttt gcctaattct agattgaatt tcgcaagctg tgcttgtttg
    14221 ttgtcccaat ctttttttgc tgtcttgatt tcagcaacca tcaaagagac atcggtttga
    14281 gtcagtgttt cttttagccg taaaaactcc tttgctgcct cactctgggc ggccaaagga
    14341 gttaactgtt cttccaattc gtggatgatg tcttggacgc ggctgaggtt gtcttctgtt
    14401 tcaaaaagct tctgttctgc tttctttttc cgctgcttgt atttcaagac accagctgct
    14461 tcttcaaaga tcccacgacg gtcttccggt ttactactga aaatcgcttc tacttttcct
    14521 tgagaaatga ttgagaacga ttcttttcct aatccagaat ctaagaacaa ttcttggata
    14581 tcttttaatc tgcagctttg tttattgatg aaaaactcgc tttccccagt ccgtcgataa
    14641 cggcgggtta cactgatctc attgtactcc aatggcagat aatgatcact attgtctaaa
    14701 atcactgtga cttctgcaat attcagctgc ttacgggtat cggaaccggc aaagatgata
    14761 tccggcattt ttccgcctcg cagactttta gcagattgtt ctcctaatac ccagcggata
    14821 gcctctgtaa tattgctttt tccgctgcca tttggtccaa ccactgctgt gacgctattt
    14881 tcaaaatcaa tgactgtctt gtctgcaaat gatttaaaac cggctatttc aattcgtttc
    14941 aaatacaaag ctttccctca cttactttta cagtgctgcc aatgcatttt ccgccgcagc
    15001 ttgttctgct aattttttcg atttcccttg tccagtgcca atcaattgat catctacata
    15061 aacttcgatc caaaatactc gttcatgagc tggaccttct tcattgatca atcggtattc
    15121 gattgataca tcacctgatt tttgcaagac ttcttgtagt tttgttttat gatccatctc
    15181 atgtgaaaaa gcacctgctt tgactttagg aaaaatcact ttttcgatga atgcatgggt
    15241 cgtgtcgaat ccctggtcaa gatataacgc gcctaaaaaa gcttcgaata ggtcgcaaag
    15301 caatgctggt cgcgtacgtc ctcctgaatt ctcttctccc ttgcccaaca aaacatattg
    15361 atcaaagtga cattctttgg caaatttcga caagctgtct tcgcgaacga tcgcagctcg
    15421 tgtttttgtc aattttcctt ctggcaaatg aggatataaa cggaataaat actgagaaac
    15481 catcagttct aaaacggcat ctcctaaaaa ttcaagtcgc tcattatccg ataattgcag
    15541 attgcgatgc tcattcacat aggatgaatg ggtaaaagct tgttcaagca aatttagatc
    15601 gtgaaaaacg atgttatagt tttcttttag ttcttttgtc agctggttgt ccattttcca
    15661 cattcctttt atggatattt tttcgatact tccttattat actggttttt tgtcaaattt
    15721 gaaatggttc ggtcaaaagg taagtaaagc tttaataatc ctagtgattg acgagaagtt
    15781 gtgaaaaatt cgtttagttt cgagtatgaa gaaaaaattt tgtgaaaatg gctttccata
    15841 tttttgcata aatttgactt aataccgaga aactggactt tgaacaccgt ttattggaga
    15901 agtcatgaaa tcagcgcctt gacctgaata ttagagaaat aaacagaaaa atatcaaaaa
    15961 accgaaaaag gacatcgccc tttttcggtc atttttagtg attatgaacg tgaatctgct
    16021 gtaacagaaa catttgccca tgggtttggt acatcataag accaatccca gtctttgaca
    16081 cggtcgttga ctggcaatac ttcattacgg aacaacgttg gaatcgcaaa tgcttcttca
    16141 aatgcatatt gttgccattt ttgatatgct tctttttggt actcttcatc aaatgctttg
    16201 cttgaactga tatcgttgat caattttgta ttttcttctg attcataacg agtgtagttg
    16261 aacgcagcat ttggtcccca tagtccactt ggatttggat cagttcctgt attccaagca
    16321 gcttggtaaa catcaatttc aggatcatcg ttttctaatt tatcatagaa agaattgaat
    16381 tcgatcagac gacctgttgt gtattggaca tccaaaccaa tttctttcca ttgttggata
    16441 tagtaatctg ccaatggttg cgctgtttcc ccaccggaca tcgaagcaaa gttgattgtt
    16501 agtttatttc catctttgtc ttctcttaaa ccatcgtcat ccacatcttt gtagccagca
    16561 tcatctaata gttttttcgc tttgtctaga tcttgtgtga atcctttcat ctcatcatca
    16621 tggtatgact ggaatactgg aggaatcaat gaagtcgcat tgcttctcaa acctgcatag
    16681 aaacgttcac ctactgcatt attatctaat gcatagccca ttgcttgacg taatgattta
    16741 tcagccattt ttgaatctgg attgtattcc acactctttg tatctgcatt ccatttacct
    16801 aacttgaatc ctagataagt ataagaagtt tgttcacggc ctaagtttgt atagccagca
    16861 atttctttat aggtatcgta agtatctgtt ggcatttgga aaatcatatc atataattta
    16921 gagttcaatg cttcagttgc acttgccatc ggtacagatt tcatcgtgat ctttttcaat
    16981 ttaggcgtac cattccagta gtgctcgttt ggcaagtatt ctactgattc tcctgcaacg
    17041 atattactca tgtaataagg gccaaaagtc actggatttt tacgtacttg atcgcttgat
    17101 tccatatctt tgattgctac accttctaac gtatgttttg gtgctgcata tgaccagata
    17161 ccaccacccg attgaagcat gcttgggttt acttctttat attcgatttc aactgttttg
    17221 tcgtctattt ttttgattcc ggagattgtg tcggcacttc catcatgata ttcttccata
    17281 cctacgatgt tacggaaagt gtcgtcataa cggataccag tgtaatcttt attcccaatg
    17341 atttcgtaag ggaaaatcac atcgtccgcc gttacatctt caccatctga ccatttgacg
    17401 ttgtctttta ctttgattgt cgcttttttc gtatcttcat ctagatcaag cgttgcagct
    17461 ccgtcgtctg tgattctgaa atcgtcatcg tttgcaaata gcggttcatg agaaggagcc
    17521 ataaaagctg catcatatga atcttggtaa aactcccact ggaacaatcc tttaaactga
    17581 ctgtccgttg cgaccgcagc ttctaattcg ccgtcttcga ttgcatcatc attatttttt
    17641 gttgcaattg gaaaagttaa ttcctctttg ttattgccgt tgcctccaga gttgtttcca
    17701 gaggtatctg tacctccgcc cccgcatgct gcaagaacga cagcagacat cagcgctgca
    17761 aatccaaaaa tcgttttctt cattcctatt tcccccttcg cttctatact agcctaaccg
    17821 ttggcgcgca tctgcagaac gcttgaatgc ttgtccaaca taattgatac tcaacatcaa
    17881 aactaaaata aggattgatg cgggtaacca gatccattgc cgattcacta agacttctcc
    17941 gttacttgca taaccgatca atgtacctaa actaggtaca tttgccggca agccgaaacc
    18001 taagaaagtc aaagttgtct cgataccgat gtttgccgca aagttcatcg tcaaattagt
    18061 aatgattaat gaacttagat tcggcatgat ttcgcggaac atgattttaa aatcattggt
    18121 tcccattgtt tttgaggcac tgacataatc tcttcttgct tcagataatg tcttgcttcg
    18181 gaataatcgg gctttagcta cccaataaaa tgcactcatg atgaatataa aggaccagac
    18241 attgtatctg gaaataatcg atacgaagac gataatgatc agcatgatcg gtagaatcat
    18301 gataaaatca acgatccgca tcaagatgtt gtcaatgatc cctccgtagt agccagagat
    18361 gatccctaat ccaaccccga tgatcgaagt aaggatcgtg atcgcaaatc cgatcgtaac
    18421 agagtttcta gcacctatga tcaattgtcc taatacatca cgtccgcctt catctgctcc
    18481 cagaatgaac ttttgtcctt gtgtcgaaac tgttcctggt gcagcgtatt tgtcgaagat
    18541 actaacatac atcactttgt cttgatcggt cagcatcgca ccgataaaga tcactaataa
    18601 taaaaccact agcaaaccta atgaaaaaat agcaatttta tcttttttaa attctcgagc
    18661 aatcattcgt atccccatag gaggaatact ttcttgaact gctgtattgt ttttttcact
    18721 catcgatcca ttctcctttc tgattggttt tattgaatcc gaatacgcgg gtcaacgata
    18781 ctcatgatga tatctgaaag taatgtcccc actagcgtcg cgattcctaa aatcaatact
    18841 aaggatgtga tcacggtata gtcccgttgg ccgatggaat caataaatag ttttccgact
    18901 cccggataac caaaaatctt ttcgataact acagaaccag caatcaacga agtgatttca
    18961 taccccattt gagaagccat cggcaacgct gcatttcgga aaatatgtcg actgaatact
    19021 ttattcgtcg gtaccccttt ggaacgggct gttctgacaa aatcgagcga tttcgaatcg
    19081 attacttcac ttcgcagata ttggattgtg acggcagtcg ccaaaaatgc ttgggtaatt
    19141 gatggtaaca gaatatattt caaacggtct atccattgag tgataaatcc accttgcaaa
    19201 cctagtgttt gtgatccact tgttgggaac cagcctaaac gataaccaaa aacaaatagc
    19261 atgatcaagc cgaagataaa taaaggaaca gcaaaactaa agaagttgta gattacgaca
    19321 atcttatcca agattgaatt ttgataccgg ccagcaagca tccccagagg cacagcaatc
    19381 aaataagtaa ggacaactgt caagatcgat agatataaag tgttgatgat ccgttcgccg
    19441 atcaagtcag caacagagcg tttgtataag aaactttgac caaaatcgcc ttgcaaagca
    19501 tttccgatcc aacggaagta ctggacatac catgggtcat tcaagccagc tgcttgtcgc
    19561 atctgttcga tcactgccgg atcctgattt ggattgatca aaccggtaaa tggatccccc
    19621 ggcatcgctt gggctaataa gaagatcaga acacttaaaa taataacttg cgggatcatc
    19681 aataaaacac gtcgcagaat cgttttccac attagcttgc acctccatct ttcaacgcca
    19741 ctttgtgtgt agttgtgatc gtacgcaaat cgtagactcg accagtcgca tcatagtatt
    19801 ctttttggtt ttggatatac tcttgttcta ctcttcgtcg attttctttg tggctttccc
    19861 tattttctac atcgatttgc ggaatagctg aaagcagacg tttcgtatag atatgtcgcg
    19921 ggtcattata aatgtcttct cttgtaccga tttctacaaa tcgacctttg tacataattg
    19981 cgatattgtc gcacatgtgt tttactacac ctaaatcatg tgaaatgaat aaatagctga
    20041 ggccgtattc ttgctggatt cgtttcatga aattcaatac ttgcgcttga acagatagat
    20101 ccagagcaga tacaggttca tcggctacga tcagttttgg attggtagca acagcacggg
    20161 ctactcctag acgttgacgt tgtccgcctg aaaattcatg aggatattta tatagcgcat
    20221 cttccggcat cccaacgata tctagcagtc cttttacctt tttcttttct tcttgatcgc
    20281 ttaaccgttc aaaatttcgg atgggttcag caatgatatc caacactcgc tttttaggat
    20341 tcaaactgga cattgaatct tggaagatca tctgcacatc cttgttataa cccatttttt
    20401 tgcggttgct tcgcttcgtt acatcttttc cttcatacat gatttgtccg gctgttacct
    20461 tttctaatcc cacaacggct tttcccgtag tagattttcc agagccagac tctcccacta
    20521 aaccatatgt ctttcctttt tcgataataa aatccacacc atctacagca tatacatgat
    20581 ccgttactcg attgaagaat ccactgcgaa tagggtaatg aactttcagg tctttgattg
    20641 tgattaattc tgtcatctac acctctcctt tttcgtctct aaagtggaaa tgcttatagc
    20701 aggagcaacg taccaagtgg ttcggtgcga cttcatgaag gataggctct tcttcatgat
    20761 cgcttgctgg tatccatgga atacgtggtg caaaccgaca acctttacga ggcatgtttt
    20821 tcagagaagg aacgacacct tcgatcacat ggagttctgc atcatgattt ccttcttgtg
    20881 gtatagagtt cagtaaagag cgtgtataag gatgcttagg atgatcaaac agttctttta
    20941 cacctgctac ttcaacgaac tgtccgccat acatcactgc cactcgatca gccatttctg
    21001 ctacgactcc taagtcatga gtgatcaaga tgatccctgt ttttgtttct tcctgaaggt
    21061 ctttcagtaa atcaagaatc tgtgcttgga tggttacatc tagggctgtc gtcggttcat
    21121 ctgcaatgac gattggaggt ttgcaggcga ttgcgattgc gatgatgaca cgttgtctca
    21181 taccgcctga tagttcatgg ggatattggc gtccaactct cgcaggatta ggaatcccta
    21241 cttgttctag caattctagt gcacgagctt gacgctgttc tgcattcatc tttgtgtgat
    21301 aggaaagtcc ttctttgatc tggtcttcga ttctcatgag aggatttaat gcagacaacg
    21361 gatcttggaa aatcattccg atatcatttc cgcggatttt attatagagc gtttcgttca
    21421 ggtcgattaa attcagatcg ttatataaaa tctcacctgt aatttttgtg ttattaggat
    21481 caagtaatcc cataatcgta gtagccaagg tacttttccc gcacccagat tccccaacga
    21541 ttgctaaaat ctcgtttttg tctaaagtaa atgaaacgtc atctactgca tcgtaatagt
    21601 catctttgag tcggaatcct gtatgtaggt tctgaacttc taataattgg ttatcggaca
    21661 atcgaatacc cctctctgct tcctaaatgg aagtttttct taataaaaaa ggacattaca
    21721 cagaataaat cgttttataa
//
LOCUS       DAYYCY010000032        20460 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000032 DAYYCY010000000
VERSION     DAYYCY010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.032"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..449)
                     /locus_tag="ACHAZB_000975"
     CDS             complement(<1..449)
                     /locus_tag="ACHAZB_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM8613666.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIA"
     gene            complement(552..992)
                     /locus_tag="ACHAZB_000976"
     CDS             complement(552..992)
                     /locus_tag="ACHAZB_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM8613667.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(1049..1459)
                     /locus_tag="ACHAZB_000977"
     CDS             complement(1049..1459)
                     /locus_tag="ACHAZB_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFM8613668.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(1639..2073)
                     /locus_tag="ACHAZB_000978"
     CDS             complement(1639..2073)
                     /locus_tag="ACHAZB_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8613669.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(2173..3021)
                     /locus_tag="ACHAZB_000979"
     CDS             complement(2173..3021)
                     /locus_tag="ACHAZB_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM8613670.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            3410..5176
                     /locus_tag="ACHAZB_000980"
     CDS             3410..5176
                     /locus_tag="ACHAZB_000980"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HFM8613671.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            5278..6537
                     /locus_tag="ACHAZB_000981"
     CDS             5278..6537
                     /locus_tag="ACHAZB_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM8613672.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            6684..7985
                     /locus_tag="ACHAZB_000982"
     CDS             6684..7985
                     /locus_tag="ACHAZB_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8613673.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            7988..8839
                     /locus_tag="ACHAZB_000983"
     CDS             7988..8839
                     /locus_tag="ACHAZB_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFM8613674.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            8852..9667
                     /locus_tag="ACHAZB_000984"
     CDS             8852..9667
                     /locus_tag="ACHAZB_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HFM8613675.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            9818..10984
                     /locus_tag="ACHAZB_000985"
     CDS             9818..10984
                     /locus_tag="ACHAZB_000985"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8613676.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            11214..11861
                     /locus_tag="ACHAZB_000986"
     CDS             11214..11861
                     /locus_tag="ACHAZB_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFM8613677.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            11945..12889
                     /locus_tag="ACHAZB_000987"
     CDS             11945..12889
                     /locus_tag="ACHAZB_000987"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HFM8613678.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            13024..14475
                     /locus_tag="ACHAZB_000988"
     CDS             13024..14475
                     /locus_tag="ACHAZB_000988"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HFM8613679.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            14496..15332
                     /locus_tag="ACHAZB_000989"
     CDS             14496..15332
                     /locus_tag="ACHAZB_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFM8613680.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            15332..15991
                     /locus_tag="ACHAZB_000990"
     CDS             15332..15991
                     /locus_tag="ACHAZB_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFM8613681.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSIHQS
                     I"
     gene            16117..16806
                     /locus_tag="ACHAZB_000991"
     CDS             16117..16806
                     /locus_tag="ACHAZB_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8613682.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PKDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            16812..17768
                     /locus_tag="ACHAZB_000992"
     CDS             16812..17768
                     /locus_tag="ACHAZB_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HFM8613683.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            17812..18324
                     /locus_tag="ACHAZB_000993"
     CDS             17812..18324
                     /locus_tag="ACHAZB_000993"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8613684.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSGVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(18580..19128)
                     /locus_tag="ACHAZB_000994"
     CDS             complement(18580..19128)
                     /locus_tag="ACHAZB_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFM8613685.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(19217..20398)
                     /locus_tag="ACHAZB_000995"
     CDS             complement(19217..20398)
                     /locus_tag="ACHAZB_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HFM8613686.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
BASE COUNT         6765 a         3679 c         3977 g         6039 t
ORIGIN      
        1 gcaatcgaag cgccattttg aagctgttca aaaaaatcgt ttcgctcaat ataagtatgg
       61 atatattgta aaacaggatc tgcatctttt ggctgatgct cgtaaaaatt aataacggac
      121 tggatcatgt caaatgtttg atgaataagc atcgggatat actttgtgaa tgccagatag
      181 ataaggtaaa cgatcagtgt ataaatcatg atagctaaaa cacctgtagg gattcggaca
      241 aatcgctgaa tgatttgaac aagatgaatc atcaaataag taaagataaa tgtgagcaaa
      301 atggttgtaa tcatacttct aactaagaaa agaacaaaaa cgacaaataa taaaacagtg
      361 agacggcgta ttttttcatt cgccaaaaaa cgctgataaa cggtcaaatc gacaacctcc
      421 aaataacaaa aaataaacac gttattcatt atatgatgga atatgaaaat aagaaagagc
      481 aaaaagaaaa accgctaaat gacgttatct tatgagttgg ttttcctcat aaaatcgttc
      541 atctagcagt ttcagccctt tttctggtct tgatgaaaat tatctagcat ttttcgggtc
      601 agatgttgca gtgtatccaa ttcttcctgt tcaagtggaa gataagactt gatcttttgt
      661 ggaatacctt ctgcttgttt ttgcaacgca gcaccttgag cagtcagcgt aacatttacg
      721 attctttcat cacccgtttc acgctgacga ttaatataat ttttttctgc taactttttt
      781 agtaagggag tcagcgtacc agaatccaga cacaaatcat ttcctagctc tttcactgaa
      841 gtctttttct tttcccataa aactaacatc acaagatact gggtataggt aagattcagc
      901 ggcttgagga ggggagtgta gtgtctaatg atttctttag aggcggcata taaaggaaaa
      961 catacttgat taactaaata tagatcttcc atttttccac ctccgctgtt ttactatagt
     1021 gtatcatagc ggatttacac aaaaaagtct attcactaac tgctttcaga gtaacttcta
     1081 tatttccagc tgtagctttt gaatacggac acactttatg cgctgcctct aataattctt
     1141 gcgtttcttc cagagaaaga ccttcgatag atccttcaat ctctacacct aagacaaagc
     1201 tcgtttcact ttctgaataa agggaaactt gtgcactgac agtagaagcc ccagagatat
     1261 ttttttgctg tttgatcaat tctaatgcac tgttaaaaca tgcactgaaa ccagctgcaa
     1321 acaattgttc agggttggtt gcattttcga cgcgtttacc aggagctgca atattcaatt
     1381 cgaatgtacg atcgggatta tgcacttctc cagagcgtcc gcctgtgttg atcattttcg
     1441 tagaatacat ttttttcata aaattaccac ctttaattat attttataca attgaatttt
     1501 acacaatata attgtggaaa gcaaagaaat ggattggcat aagataaata agagaaagca
     1561 catgaaacag acgcgctttc tcgaatagca aacatataca aaaaatatca gagacaaaaa
     1621 ataatctcat cataaacatt acatatattt ctcaaaaagt tgttttcttt cttcaaaaat
     1681 tacttcacga tggacctgat gaagcatcca taaataacca aaaggatctc tgaacattga
     1741 gttagtcaca ccaaagtcca tcatttctgt catttcttga atctctacac agccatttcg
     1801 taatgcttta ttatgtgtgt cctcgatatc agagacgatg atattgaacc agatcgtatt
     1861 tggatgttca gcagtaggag cagtcaaacc aagagacgtg ttttcatcca gcatatggaa
     1921 tctcgtacca tacaaggtaa aaatgacttc attattgcct ctagggtaat ctgttgcctc
     1981 aatcagttgt atttcatcaa agattgtttg atacagttct agtgctttca aactatcatt
     2041 taatacaaaa tcgatttccg ttcctttatg cataaagatc tcctctcctt ttcagtcgaa
     2101 cagttactag attcttttat tataccagct aaagaaaacg cattcaaaaa aatagcggat
     2161 ttacttacca actcattcga ttggatgata agtaaatccg cgtcccatta cttctgctac
     2221 gctgctgaca atgacaaagg cacttggatc gatttcttct atcaatggtt tcattcggga
     2281 aaattcatga tcagtcacaa cgatcaataa aacctctttt gactttcgat tatacccgcc
     2341 ttttgcctct agcagggtaa ggccacgtcc aatctcagca cttaagcgaa ggcggatgtc
     2401 ttccaaataa tgttcactca tgaccataac agatttcttt cgattgatac ctgtttcgat
     2461 ataagtcatg acaattgaag tgattgcaat agataaaata gcataaagaa actcttctat
     2521 tccaaaaata aacagattaa gagaaacaac gaatgcgtct gtaaaaaata agccaatcga
     2581 agtattcaag tggaaatatt ttttaaaaat gaggggaggg atcgtcgtac cgccgctaga
     2641 agagttgttt ttatataaaa tcgcaactcc taaagcaaaa atcgcacttc caaaaatgac
     2701 agacaacaac cgatctgaag taatcattgt ttctggaact attgcaatga ttaaaggaaa
     2761 aaccaagctt ccgaaaagta ctttaaaaaa gggtttttta cctaaaaata gaagagctag
     2821 taccaacata acgatattca acactaaaat aactatagct cggtcaaagc ctaaagcagc
     2881 ttccagtaat attcctagac cgctgacacc tccagcagca atatgatgag gccctaaaaa
     2941 catattgata gcgactccta atatgaagag ggcaaaaata atcttaatgt atgtccatat
     3001 aaattgtctc gtttctttca tttattcact tccttaattc attaataata ctcttatctt
     3061 attataactt acttttcacc ttaagttaac tttaattaat aaaaaaatta ggctagtcta
     3121 aattattatg tttcattctt ctagaatata cttctacgcc aaaaactgtt aaaaaacttt
     3181 tactttagaa aattggagaa attgatcgct taagataaat tcaggacact aaatagtaca
     3241 ggaagtagac taaaactttt aaaagtttta agtctatcta tttggcaagt ttaaaaaata
     3301 aaaattcggt atcaaaaaac tgttaccgat aacatttttg aaagcgttgt cttttgcttc
     3361 tctatcactt atagtgatga cgtcatatta aaacacaagg aggaaatcaa tggatacagc
     3421 tgcaatttat catcgaccgg aaagtgaata tgcatttcta tatacgaata caagatttcg
     3481 tattcggctt cgtactagga aaaatgatat caaaaaagta tatgtgctat gtggagatcc
     3541 atataccata acaactgaaa aatggtatca aaaacaacga ccaatgaaaa aatgtctaag
     3601 taccaatgtt catgattatt gggaaattga agtgacgagc gaaacaagtc gcttacagta
     3661 tgcgttccat gttgttgggg aagataacac ggactgtttt tacggtgatc aaggaatttt
     3721 tccttatcga gaaaatgtga ttactgaacc aaatttttat tttcgaatac cttattttca
     3781 tcaaatcgat cgattcacta ttccagaatg ggtaaaagaa acagtatggt atcaaatttt
     3841 tcccgaaaga tttgcaaacg gggacaaagc aaatgacccc gaacacactt taccttgggg
     3901 aagtaaagat ccagaccgag aagacttttt cgggggagat ttgcaaggtg tgatggatca
     3961 tttggattac ttgacggacc ttggcatcaa tggcatctat ttttgtccca tatttaaagc
     4021 cacatccaat cataaatacg atacgattga ttattatgag gtggatcccg cttttggcga
     4081 taaaaaactt ttaaaaaact tgattgatga agcacataaa agagggatac gtatcatgtt
     4141 cgatgctgta ttcaatcata tgggtgtcca ttcaccacag tggcaagacg ttctcaaaaa
     4201 cggagaaaaa tcgatctata aagactggtt ccacatccgt tttttcccag tagattctta
     4261 tcagatgact gacttaccag aaactgctga aaatatccca tatgatactt ttgcttttac
     4321 tccattcatg ccgaaactaa atacagcaaa tccagaagtg caaaaatatt tgctggatat
     4381 cgcaacttat tggataaaag aattcgatat tgatggatgg cgtcttgatg ttgctaatga
     4441 agtcgatcat catttttgga aaaaattcag agaagcagtt acagaaatca aaccagacat
     4501 ctatatcctt ggagagattt ggcattcctc tcaagcttgg ctgcaagggg atgaattcca
     4561 tgcagtgatg aattatgcat ttacagattc gattaaagat tattttgcaa aaaagaaaat
     4621 cacagcttca caaatggtca gtggaatgaa tcatcagcaa atgctttaca gagatcaggt
     4681 gaacgaagga acattcaact tattagattc tcatgatact gccaggatct tgaccctttg
     4741 ccaaggtaac aaagaattga tgaagtctgt gatggccttt atgtttcttc aaaaagggtc
     4801 tccttgtatc tattacggga cagaaatagg gatgactggt gaagatgacc cggattgccg
     4861 caaatgtatg atatgggaga aggaggaaca agatctagag ctattcggtt ttataaaaga
     4921 acttgtttct ctaaggaaac agttgtctaa gattatttct gaaggttcta ctcagtggct
     4981 aatagtaaat gatcgtgaag ataagctcta tttcacacgg aaactagagg gacagatcat
     5041 ctatgtttac tttaatcagt caaaagaacc ttgggtagta gaacaggaaa atgaggtgat
     5101 tcttagtcaa aattgtcagt tgttagaaga cgggaaagca gagatacgac aatacggatt
     5161 tttgatcatg tgttgaaaac tgttaccggt aacaacaaga aagcgtttgc aaatgaagat
     5221 ttgtgcccat tataataagg gtgtaacagc acgttgaata caaaggagga aatgaagatg
     5281 aaaaaaagaa gtgtgaaaca gttgggttta gggttaatct ctttaggaat cttaggcgga
     5341 ttagcggctt gtggcaatgg taactcttca gcagaagggg aagccgatga aggaaaaacg
     5401 ttaacagttt ccgttgattc tggatataaa gattatataa acgaaataaa aaacactttc
     5461 gaaaaagaaa acgatgtgaa aatcaagcta gtagaaaagg acatgttcga tcagttggaa
     5521 tcattagcgt tagatgggcc tgctggaaag ggacctgacg ttatgatggc agcatatgac
     5581 cggatcggtg cgttaggaca gcaaggacat ctggcggaag taaaactggg aaatgaagca
     5641 gcttacgatg aaacagacaa agcccaagtg acttatgatg gaaaaatcta cggagaacct
     5701 gcagtaatcg agacccttgt tttgtactat aacaaagatt tagtcgatac agcaccatca
     5761 acgtttaaag atttggagaa tctatccaaa gacagtcgtt ttgcttttga gtcagaagca
     5821 ggaaaaaata caggattttt agctaaatgg actgactttt attattccta tggtttaatt
     5881 gcaggttacg gtggctatgt atttggtgat gatggcacaa atccttcaga tatcggtttg
     5941 aataatgcag gtgctgtaga aggaatctct tatgcaacag attggttcaa aaatgtttgg
     6001 ccaaagggaa tgcaagacat aaaaagtgca ggagacttcg ccagccagca attcatgtcc
     6061 aataaaactg cagcaatcat tgacggtcca tggcaagcgc aaacatacaa agaaaacaat
     6121 atcaattatg gtgtagccaa aattcctact ctaaataatg gacagcctta tcaaccgttt
     6181 ggcggaggaa aaggatgggt agtcagcaat tatgctaaaa acaaagacct ctctcaaaaa
     6241 tggcttgatt atgtaacgaa tcaggaaaat caagaaaaat tctttgaaat ggtaaatgaa
     6301 atccctgcaa accaacaagc tagggaaaca gcaaaggcta aaaacgatga attaacaaca
     6361 gctgtgattg aacaatatga aacagctcaa gcgatgccaa acatcccaga aatgggcgaa
     6421 gtttggacag gtgcagaaaa cttgatgttc gatgcagcat caggttcaaa aacaccaaaa
     6481 gaatccgcag atgaagcagt aaaaacaatc agtgaagcca tagaacaaaa atactgatta
     6541 aaatgataac cgtccatttt tataaaaaat ggaacaggtg cttcagataa aagtggcaag
     6601 agcgagatgg aaaattatct tgctcttgtt gccattatct taaaagcagt ttgcggttta
     6661 gcaagtttag agggggcatt cagatgcagc aagataaaac aagcaatatc aacgaaaaaa
     6721 aaatcacgct ttgttcactt attccaggga tggggcagtt tgtaaatggc caagctttta
     6781 aaggtatcgt ttttttcgcc atattcgtcc tattcattgg tcagatgctt ctgggcggat
     6841 ttcatgcatt ggtaggattg gttactttgg gaagtatacc aatggaagat cattcattat
     6901 ttattttgat tgaaggcact ttgcaattca tcattacaat cattttcttg attttttacg
     6961 gtttgaatct tttagacgct tatcgagtag cgaagatgcg tcaagagggc aaaaaaatca
     7021 atcggacgat gaaggaagtc atacataata taggtgatga aggttttcct tatctattga
     7081 cacttcccgc ttatcttctc atgattttta cgatcatttt cccagtccta gttacattgt
     7141 tcactgcatt tactaattat gacttcaatc atattccacc agccagttta attgattggg
     7201 taggggctaa aaactttttt agtatcttct ttttaagctc atatcgtaat actttttttg
     7261 cagtcttttc gtggaccgtg atttggactt tatgtgcaac aactttgcaa atcacccttg
     7321 gtattctcac agcggttgtt gctcaccaga aatttattaa attcaaacgt attttcggta
     7381 tcattttctt attaccatgg gcggtcccag cttttattac tatcatgagt ttttcgaata
     7441 tattcaacga tagcgtaggt gccatcaata ctcaagtgat tcctttcata aaccagcttc
     7501 cttttgtaga tatcagtgca atctcttgga aaacggaccc gaactggacg aaagtagcaa
     7561 ttatcttagt gcagggctgg ttaggatttc cctatatcta tgtgatggtt acaggaatct
     7621 tacaatcgat ttcagaagac ttatatgaag cagcaaaaat cgacggagca aatgcatggc
     7681 agcgcttttc aaacatcacg cttcctgctg tcttcctagt ggcagcgcca acatttatta
     7741 cacagtatac ggggaatttc aataatttct ccatgatcta tctgtttaac aatggcggtc
     7801 ccggaagcgt aggcggagga gctggttcta ctgatattct tatttcctgg atctataaac
     7861 ttacgactgg aacttctccg cagtattcta tggcttcagc aatcacgttg attatctccg
     7921 tcatcgttat ctgtgtgtca ttgctggtat ttaaaaagac aaatgcgttc aatatggggg
     7981 attaacaatg aaaaaatttc attcgagtgc tcgattccaa aaacgacttt ctcacttttt
     8041 tacttacttt tttcttttaa tactgtcagt cattatcatc tatccgcttt tgatcacagc
     8101 aagtactgcc ttcaagtcag gaaatattgc agcttttagt ttagatcttt caagtgactg
     8161 gactttagga aactttactc ggctattcag tgaaacactt tacgggactt ggtataaaaa
     8221 tacattgatc atttctgtcc taacaatgat cgtacaagtg actgtagtaa ctttggcggg
     8281 ctatacttat agtcgctatc gttttatcgg ccgcaaatca agtttgatgt tctttttagt
     8341 gattcagatg gtaccgacta tggcagcatt gactgccttt tatgtcatgg ctttcttatt
     8401 agaagcgctc gataagtact ggttcttgac aatgatctat ataggcggtg gaattccaat
     8461 gaatacttgg ctgatgaaag gatactttga cactgtacca atagatttag atgaatctgc
     8521 taaacttgat ggggcagggc atttcaggat ttttttccag attgtattac cgcttgtcaa
     8581 accgatgctc gccgttcaag cattatgggc ttttatggga ccttttgggg atttcatgtt
     8641 agctaagttc ctgcttcgaa cacctgaaaa tcagactgtt gctgtcggct tacaaacatt
     8701 tatttctgat gtgacaaacc aaagagtctc tctttttgct gctggggcaa ttttgattgc
     8761 actaccgatt tgtctattat tcttcttttt acaaaagaac tttgttaccg gcctgacatc
     8821 tggcggaaca aaaggatagg agcagagtat catgcaaaca tttccgttaa actatttttc
     8881 cgcattacgt acgccaactc aagtgtttgc gggacgcaaa ctattatcat ggcctaaatt
     8941 tttcttgata tttgtatttt tagtttcctt gatggtcatg cctgtgactc ttttttatgc
     9001 caatcagatc caagcgattc cattagaaca atttctatcc gttcattcgc tgatcgatga
     9061 acaaggaacc caaaaatttt ctgaacttga attatctgaa actgggttac aagcatcgca
     9121 acaaacgata gctgtgaccc ctgaaatttt ggtaggtgtg tctttatctg agaaacaaca
     9181 atccgatcat ggaactttta ttgattttga gaaggaacaa tgggtgatcc aacaaaagga
     9241 taaatctggt atacgccgct atacgatgaa ttatagccca tctttccaac cagattctgt
     9301 acgtacgccc gaagatttcc aacgattttt agaaagagaa ttttatgcca gtaatcgtcc
     9361 aacaatcatt ttatcttata gtttaagtct tggtcttgtc ctatttgtca tgacgagtct
     9421 cattttattc ggggcttcct ttttcttgtg gatgacaaga aaaagccaac tttcatccat
     9481 tcatacattc aaagagagtg caaatttgat gctgaatgtt atgggaatag gttccatggc
     9541 agcagcagtt gtagggttta tccactttga ctttatcttg atgctaagtg tccaaacaac
     9601 gataactgtc ttgctgcttt tatgggtttt tgtcaaaaca agattcaaag ataagcgtgt
     9661 cgaatgacaa gtttaagcga tttatgcgac aatgaatgtg aaaaaaatga aaaataaagt
     9721 agtttttgaa aaatttctga aaaaattaaa cttcttagtt gattcttttc ttttttagta
     9781 ttaacgtaat agggtagttt ttttaaggag gaaaatcatg ttagagaaaa ctttatacaa
     9841 tcaattattt tcacgttctt tctcgctccc tgtcgaagta aattattggg acggtacaac
     9901 gaaacaatac ggagatacgg acactcctcc ccaaatcaaa atcaatatcc atgaagaaat
     9961 cccaatgaaa gagattacaa gtaacgcatc tttggcacta ggagaagcct atatggatca
    10021 tcgaatcgag atcgagggaa gtatccaagc attgatcaac gatgcttatc agcaagcaaa
    10081 cagcttttta cgaggaaaag attatctaaa atggctgcca aagaaagaaa aacatacgaa
    10141 acaaaaaaac aaagaagata tccatgcgca ttatgactta ggtaacgact tttataaact
    10201 ttggctagat ccaactttga cttattcttg tgcttatttc aatacacctg aagatacgtt
    10261 ggaacaagca caaatcaata aagtccatca tatcttagac aaactcttta tcaaagaagg
    10321 cgaaacgttg cttgatatcg gttgtggctg gggaacattg atgttcactg ctaccaaaga
    10381 gtataatgtc aaggcaactg ggatcacact aagtgaagaa caatacgatt tgattaccaa
    10441 aagaataaaa gaagaacatt tagaagataa atgccgtgta ttattaatgg attaccgcga
    10501 actaaaaggc gaaacgtttg atcatatcac aagtgtaggt atgttcgaac atgtaggttc
    10561 tgaaaatctt gaaggttatt tcaaagtggt aaaagattta ttgaaaccaa aaggaacagc
    10621 tttgatccat ggaatcagcc gacaacaagg cggtgcgaca aatgcttgga tcaataaata
    10681 tattttccct ggcggttatg tccctggtgt tactgaatta gttgataata ttacaaaaaa
    10741 cgatttgcaa ctgattgatt tggaaagctt acgtcgtgac taccaattga cattagaaca
    10801 ttggacaaag aattttcatc aagtccaaga tcaagtcacg aaagaaatgg gtgattcctt
    10861 ctatcgaatg tgggatctat atttacaagc ttgtgcagct tcatttgaat caagtaatat
    10921 cgatgtgatt caatatttgc ttgttcaacc tggaaacaat gacatcccaa tgcatcgtgc
    10981 ataattgagg caaaagtact gtttaaagac tatttaaaaa gaagctaagt tttatgatgc
    11041 tccagaagtg aacttttttg tctaacttct aggattgtct ctaaagctta gtttcttttt
    11101 ctttatattt tcataataaa agtttggttc tttaattttt aaacatgaca gaaaattttt
    11161 tcttcgttat acttgtaata gaatactatt ttataacaaa ggggatcgaa tcaatgaaaa
    11221 aaactgttat ttatactagt ttatttgcag ctttgttttt agctgcttgt caatcaaata
    11281 ccaatcaagg cacatctggg aatacgtctt ccagtagcga acagcgctct tcacaggtat
    11341 cggactcctc aacaaaagca agcacatctt cgtctagcca gacaaaaagt tctagtgaag
    11401 caagtactca gtcagatatc aaagtgtctg cagctcgtgc gatcgaattg ttccaagata
    11461 aatacccaga tactgcaatt actagtttgg aattagatag tgattgggga tcttacttct
    11521 acaagataga aggtgtcgat gatcaaaaag aatacgaagc aagaattgat gctcaaaatg
    11581 aaaaaatgga gacagagaat ccagaaactc tagatcgaga tgaacaaaat ggtcaaaaga
    11641 aaaaagaaga tggtctggat gttgataaat tgatcacgat tgaggaagca gcaaaaatcg
    11701 ccgtcgacaa agtaggaagc ggtacggcaa cagattggga tttggataaa gatttaggta
    11761 ctacttattg ggaagtcaaa gtaaaagacg gcaatcaaag tacatcagtc aagatcaata
    11821 gccaaacagg cgaagtactg tcaactgaac aagatgatta agaatttctt cggaaaacaa
    11881 gagttgattt ttcttttagt catgatacac tagacaaaat aacttgtttg aaggagcgat
    11941 ttacttgagt ttagaaacga cctttgccaa tcatatcttt gccaatcctt tgatgaatgc
    12001 atctggtgtc cactgcatga ctacccagga attagatgaa ctggctcatt cagaagcagg
    12061 agcatttatc actaagagct gtacgatcaa tgaacgaaaa ggaaatcccg aaccacgcta
    12121 ttttgatgtt cctttaggga gtatcaattc tatgggactt ccaaacttag gtttttctta
    12181 ttatctagag tatgctcttg cgtatgaaaa agtgcaggaa aaccaaaatc aaccgttatt
    12241 tttttccatt gccgggatga gtgtccagga aaacttggaa atgcttgaga aaatagaaaa
    12301 aagtgggttt aatgggatca ctgaattaaa tttatcttgt cccaatgtgc ctggaaaacc
    12361 acaactagct tacgattttg aggccactta tgaaacactg aaagaagtat tcagcatctt
    12421 ttctaagccg ctaggaatca aattgcctcc ttattttgat tttgctcatt ttgatcagat
    12481 ggctgatatc ttgaatcagt ttccattgac ctatgtcaat gctatcaaca gtgtgggcaa
    12541 tggtttgtat atcgatacag aacaagaagc agtagtcatc aaaccaaaag aagggttcgg
    12601 cggcattggc ggagagtata tcaagccgac tgctttggcg aatgttcgcg ctttttatac
    12661 acgtttgaaa ccagaaatcc aaatcattgg tacaggtggg attcggacag gacaagatgc
    12721 atttgaacat ctgctatgtg gcgcaagcat gttacagatt ggtaccgagc ttcataaaga
    12781 agggccggaa attttttcta gaatcataaa agaattaacg cagattatgt cagaaaaagg
    12841 atatacgtca attgacgaat ttaaaggcaa attacgcacc atttcatagc aaagagacga
    12901 aggtcatgat tgatcttcgt ctttttttat aaaaaacact cgtctgttca ttcctgtgca
    12961 agcgtactca ttgtatgtta gacgaacttg tgggaaaata acagaggcaa aaaggaggat
    13021 aatatgaatg atgcaacaga attagcaaaa gagcttaaag aaaaagaatt tagttttcaa
    13081 gaactagtta cggatataca gaaaaaaata aagaagcaaa atcatgaatt gaatgcgttt
    13141 gtcgcgcttg aaccattcga gagtaattcc cacgagctat tgattcccca gtcagaaaaa
    13201 gggccattgg ctggtatccc atttccttta aaaatgctcg gtcaagaaaa aaagggatgg
    13261 ctagctactt caggatctcg tttatttgaa acacaccgag cttctcatac atctaattat
    13321 gtgcggcaag cagaagcgat aggtctagtt ccttttggac aaaccaatgc gccagagttt
    13381 ggtttcaaaa atattacaga cccagtcatc tatggcccag caagaaaccc ttggaacctt
    13441 gaatgtacac cgggcggatc aagtggagga gctgctgctg cagtttcatc aggaatcgtt
    13501 cctttagctg gtgctagtga tggcggtggt tctattagaa tacccgcttc gttttgcgga
    13561 ttgattgggc tgaaaccttc gcgagggacg atgccagttg gtccatatgc ttggagagga
    13621 tggcaaggag ctgctattga tttcggcttg acagtttcta tgagagacac ggaggcttta
    13681 ttttatggga tgagaagtat ccattccggt gcgccatatc aagtatcgcc agttgagtgg
    13741 caaacacatc caagaaaaaa acggttgaag atagcttttt gtgttgattc accgatccac
    13801 tcacagatta gtgaagaggc agtacttgct gtaaaaaaag ctgtagattt ccttggaaaa
    13861 gaagggcatg atttgacaga gattccttac ccactagatg gtcgtaaact gatcacgtct
    13921 tattatcaga tgaatggcgc agaaacagca gcaatgatcc gctcgattga aacaggcatc
    13981 cagcgagctg tcagaaaaga agaacttgaa cccatttctt ggactttgtt ccaatatggt
    14041 cagaagctct ctgccgctac ttacatcgat tctctgcatg tatgggatga agcagcaatt
    14101 gtcatggaaa acctacatca agaatacgat ctattccttt caccaacgac tgcatttacc
    14161 gcccctaaaa tcagcgaaga tttgcaaagt gatgcgataa gagcacagat ggaacacatc
    14221 agtgattgtt ctgaagtaga aggtttagac gttgtttctt ccatgtttga ggagagcttg
    14281 aagatcacac cttatacaca gttggctaat ctgactggac aaccagcaat cagcttgcct
    14341 actcatttga caaaagaaaa catgcctcta ggaatccaat ttatggctgc cagaggaaga
    14401 gaagatttgc tatttcaaat cggctatcta tttgaagcaa atcatcagtt ccatttacca
    14461 aatccatgga aatagcagaa acaaggagaa gaagaatgaa gaaaaaaaga ttgtttgctc
    14521 taataactag tgcctttttt attttaagcg cttgcggtac agctgccgct aatcaggaag
    14581 ataacagctg gagtaatgta gaaaaaaaga aagaattagt agtagcgact tctggcacac
    14641 tgtttcccgc atcttattat aatgatcaaa atgaattagt tggttatgat gtagatttgg
    14701 caaaagaggt ggctaaacgg ttaaatgtca aaatctcctt taaagaatac aacgtggatg
    14761 gacaaatcac ttctgtttca aaaagagaat cagattttgc tgcgaacgat tttggtatca
    14821 caaaagaaag agaaaaaaaa ttctcccttt cagagacttt caaatattca tttgacagta
    14881 tgatcgtacg aaaaaaagat gattcaggaa tccactcact tgaagatctt aaagggaaaa
    14941 aagcagctgg cgaacccaat actagctata tgcgtttagc ggaaaaacac ggagctaaat
    15001 tagtcacata tgacaatgcc acgaatgatc agtatttaag cgatgtagca aatggtcgaa
    15061 cagatgttat tctaaatgat tattatctgc agaaaatggc cgtatctgct ttaccagata
    15121 tccctgtaaa aatactggag aacgtttatt tcaatgaaaa cgaaagtggt tttttattga
    15181 aagaaggaaa tgatgctctg aaaaagaaaa tcgatggcgt attaaaagag atgaaagaag
    15241 atgggacgac aaagaaaata tctgaaaaat attttcaaac tgatgtttca gtcaaaccga
    15301 aagaaacgat ggctgagtcg gtgagtgagt gatgtacttt cccctaattg atttttccct
    15361 catgtggact tcacttcctt tcgtactgca aggcttggta tacacattag ggattggctt
    15421 cgtgtcgttt cttttaggaa atttggtagg gttgcttttg acagttttag gtcttttgga
    15481 ttggctgcct ctgaatgtat ttattcgttt ttatttatcg ttctttagag gcattcccgc
    15541 actagtcctg ctgtttctgt tatatttcgg gttaccttat caattaagtg ccttaacggc
    15601 ttcagtcatc tgctttacga ttaccagcag tgcatttatt ggtgaaatct atcgaggatc
    15661 actagcaggc gtttcttcag gtcagtggga cgctggttta gcttctggtt tttctttctt
    15721 tggggtattg aaatatatcg ttttgccgca agcttttcga atctcagtac cggcactcag
    15781 taacgtagct atggatctag tgaaaggtac ttcgttagca gcaacaatca cagtgccaga
    15841 aatatttcaa aaagcaaaga tcataggcgg tcggacattc gattattttt ctctctatat
    15901 tttagttgca ctgatttatt gggtcatttg tatcgggatc gaaaaagtgc aacagtattt
    15961 agaaaaacgt tctatccatc agtcaatctg aaaaatagaa atggtgctag cgtggctggc
    16021 accatttcta ttttttgttt cttgtatatt ctgcctagta ttttaaaacc atttcatgta
    16081 aaatgtaaat cgaaaatatg ttcggaggta gaaaacatga agaccccgct aaccatcgaa
    16141 atagaatcct cgttatacca atattgcatc gagcaaggcg gcttggttgt agaagaagtc
    16201 acgatgcctg atgaccaagg aattgtagat acgcttagct gcttgacaaa atttgatgga
    16261 agccgagaat ggcgatgcta tgagctgaaa gtgacaaaag cagattttcg ctctacagcc
    16321 aaactgtcat ttgttggaca ttataattat tttgttttac caagtaaact atatgaagaa
    16381 gtaaaaactg aaatccctac aggaataggt gttttagtgt atcgtccgta cacggttgaa
    16441 gaggagatgc ctgcctctgg tacatttctg attgcgaaaa aagcagcaaa gaaagagctt
    16501 gctgtaccgg aagaagcttt gacaaaccgc ttcatggctt ctttattcag ggaagtaaga
    16561 aaagcaaagc aaatggagcg aggcgtaaaa tattttccaa aagatcaatt gtacaaagaa
    16621 ttgaaaagac gttcagaaaa tcaagatcca ttttctaacg aacgatatta tgaacggttt
    16681 ttagaggaaa tagctagtca ggcaatcaat gagttgcaag aagaagttat cgcgcttcag
    16741 catgattatg aatatttgag gcaacaacag cagatccgtc ggttgcctac agagccattg
    16801 gagtgaaaaa tatgtactat ccttatttaa gaggaaaaca atttgaccta ttagcattaa
    16861 aagaagcact gtcgcgagga ttgctctcca ataaaatcca accagtgatc gaaccagtac
    16921 gtgattccgc tacgcttaaa aatgtgatcg aattatttca aaaaaagcac catccaatcg
    16981 ctgtgatcca aaatccccaa gtaggacaat tcaaattatt cgatcagcat gtgcacagtt
    17041 ggacagtcaa agaaaatagc tctgtcgtcc ctgcacagat cttaacgcca gaaaatctca
    17101 gtgaagtact ggattcacct ccagcgtttc ttgttttcga tggacagcac tacccaaaag
    17161 atacagaggt gtggaaacag ttggcaggag ttgattcgaa atttttgata ccagatacta
    17221 gtcgctttcg catctggctg cccgaaaaca aaattgtgat ccgtgactct tttcaaacga
    17281 gaaagcatgt tgaaagttat gcagataaaa cagatgactt ttttagtgat gactatcttt
    17341 ttttccacgc agacggatat attggatttt ctgatttcac gatcgaaggt agccgttatt
    17401 tcgataaagg gttccctagc cgagcgcttg ctatccatct aacgtatatt gatgcttacg
    17461 gcaatatacg cgtgaaacat tttgtctctg acagtaacga ctctgctaaa gaccaagcat
    17521 tgaagtttct tgaagcagga gataaaatga aaaaatggat catgcgtcac catcatcaac
    17581 tcttgattac ttcaggaatg attgaattat tggctcttta ccagcaacaa aaattccctg
    17641 gactgggagt gttgaaaaag tggtcactta tgcatcattt ggaacttatt agtcacctgc
    17701 tagatcatcc aaaagattgg cttaaatatt attcaaaagt tcaggaacta tatgaagtga
    17761 tccaatgatt agataatgta aatgaaaaag gaatagaaaa gaggaaactg gatgtatatt
    17821 cggcaaacaa ggaaagaaga agtagaagaa gtggtagaaa tcattaatgc cgcagcggat
    17881 ttcattgctt cgcaaggctc tcctcaatgg caaaacgggc agaaaccaac aaaagaaacg
    17941 attttgaacg atattgaaag aaaagaaagc tatgtattaa tagtggaaga tcaaattgct
    18001 ggaacagctg cattagtctc aggcgtggat cccgtttaca caaaaataga tggtagttgg
    18061 aaagaatcaa ctggtcctta tttatcgatt catcgtgtcg ctttagcaga aagtactcga
    18121 ggaaaaaaac tagcaaaacc gttcttagaa catttgttga cgattgcagc cagcaaaaat
    18181 atccaagata tccgtatcga tacttatcca gctaatgaac cgatggaaaa gaccatctat
    18241 tctgctggat tcacttatca agggatgatt gaatttcctt ttgaacacgg cgaaagaaaa
    18301 gcatatcaaa taatcatcaa ttgactataa aatcatctga gcaaagataa agacattaac
    18361 actactgaaa aaaacaatca tatgatagcc aagaggttgt aacagaagtg gccagcttca
    18421 aaaaacaaga aagaatttcc gaaaatgctt ctcaaatttt tgtggaattt tagcgtattt
    18481 tacgaaatag ctgctttctg aaactccctt catcgagagg tcgtgacata gcttttgtca
    18541 cgatctctcg gcttatccta cgagtattat tcgatacatt tatatcaata atcctttttt
    18601 tattttatct gcagcttctt ggaacgctcc atcgtttgac acgactaaaa gtgaggggat
    18661 agctttcaaa cgtttcaact gtgctaaatc ttctacataa accgccagtc tggctgaaat
    18721 ctgttcagat gaatctccac gttttttcag gcgcgattgc aatgtttctt tagagatcgt
    18781 aataaagata ggacggacta ccgtcccgaa atactcatat aaatgccaaa aaccatttgc
    18841 tgtcaaaacg gcgtaacaat ttcctactgt cagtttcttt ttaatttctg ctttactgga
    18901 accatattta tgttgagcat attggtccca ctctgcaaac tcatttccct tgatcatctg
    18961 attgaatgta ttgtcagaaa caaaataata atcctcttga tctttttccc caggtcttgg
    19021 tgatcgattc gtgtaagtaa tgatcttttg ttccggctga aaaatagctt ctgcgagtga
    19081 agtttttcca gaaccagaag gaccgataaa tacatagcaa taattcattg tcatttctcc
    19141 tttgatgatt gaacaagtga aaaagctttg gaaaatattt ccaaagcttt taaaagtaac
    19201 tttgcttttt tgtattttat accagcaagc cagtcacaat agcacttaaa aagctgacta
    19261 aagtcgctgt tagcaaaatt ttcaatccgt gttttgcgac aagatttcct ttttcttcat
    19321 ttaatccctt catcgcacca gaaatgatcc cgatagaaga gaagttggca aatgaaacga
    19381 ggaagacaga tataattgct gttgtacgct cagtaaaatg gaaattacca tttgctaatt
    19441 ctgtcatagc aacaaattca tttgtaacca gtttagtcgc catgatacta ccggcatcca
    19501 ctgcttcttt ccatgggatt ccgctgatga aggcaagagg ggcaaagatg taacctagga
    19561 tctcttggaa agtgatgccg aatatcccgt ggaagatagc attgatcatg gcaatcaaag
    19621 caacaaaacc gataagcata gcagcaaccg tgatcgccac atggaaacca tcaagaatgt
    19681 attctcccaa catttggaaa aatgtctgtt ctttgttttc ttcaatgatc agctcatctt
    19741 ctttttcttc taatacatag ggatttacaa ttgaagccag aatataaccg ccaaataagt
    19801 tcaaaacaag ggcagtgatc acatatttag aatcgatcag tgccatataa gatccgacga
    19861 tcgacataga aactgtagac atagccgata cactcatggt gaacaaacgt ttctcagata
    19921 ggaagggtaa ttcctttttt atggaaataa aaacttccga ttgtcctaaa atagcagaag
    19981 ctactccatt ataagattcc aatttgccca tcccgttgat tttacttaat cctagaccaa
    20041 cagctttcat aaataatgga agaatcttga tatagcgtag aataccgata atagcagaaa
    20101 taaatacaat tggcatcaat acggaaagaa agaaaggtgc acttccttta tttgctacac
    20161 cggcaaaaac aaagtcaaca ccttgcccag caaaagcaag aagatgatca aacatttttg
    20221 ccaatacaga aacaaccgct gtaccaaaag ttgtacgtaa taatacgaaa ccaagaacaa
    20281 gctgtaaaac gaacatcaca attagtggtt tcaatttgat tgcttttttg ttactgctga
    20341 tcaaaaaagc aagccctaat gtgacaaata aaccaataat acctaataaa taactcaaat
    20401 aatcactcct attccaaatt ccgttttatt ttacctgttt tgcaaataaa atactaatct
//
LOCUS       DAYYCY010000033        20174 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000033 DAYYCY010000000
VERSION     DAYYCY010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20174
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.033"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            94..1497
                     /locus_tag="ACHAZB_000996"
     CDS             94..1497
                     /locus_tag="ACHAZB_000996"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFM8613687.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEENGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            1497..2930
                     /locus_tag="ACHAZB_000997"
     CDS             1497..2930
                     /locus_tag="ACHAZB_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFM8613688.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDAFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEKGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYASRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            3075..4001
                     /gene="trxB"
                     /locus_tag="ACHAZB_000998"
     CDS             3075..4001
                     /gene="trxB"
                     /locus_tag="ACHAZB_000998"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFM8613689.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            4215..5942
                     /locus_tag="ACHAZB_000999"
     CDS             4215..5942
                     /locus_tag="ACHAZB_000999"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HFM8613690.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            5957..6307
                     /locus_tag="ACHAZB_001000"
     CDS             5957..6307
                     /locus_tag="ACHAZB_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFM8613691.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKSKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(6399..7259)
                     /locus_tag="ACHAZB_001001"
     CDS             complement(6399..7259)
                     /locus_tag="ACHAZB_001001"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HFM8613692.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            7351..7779
                     /locus_tag="ACHAZB_001002"
     CDS             7351..7779
                     /locus_tag="ACHAZB_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFM8613693.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            7941..8117
                     /gene="rpsU"
                     /locus_tag="ACHAZB_001003"
     CDS             7941..8117
                     /gene="rpsU"
                     /locus_tag="ACHAZB_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFM8613694.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            8144..8590
                     /locus_tag="ACHAZB_001004"
     CDS             8144..8590
                     /locus_tag="ACHAZB_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HFM8613695.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(8744..10123)
                     /gene="brnQ"
                     /locus_tag="ACHAZB_001005"
     CDS             complement(8744..10123)
                     /gene="brnQ"
                     /locus_tag="ACHAZB_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFM8613696.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMPLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            10829..11800
                     /locus_tag="ACHAZB_001006"
     CDS             10829..11800
                     /locus_tag="ACHAZB_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFM8613697.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            11824..14016
                     /locus_tag="ACHAZB_001007"
     CDS             11824..14016
                     /locus_tag="ACHAZB_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing metalloprotein"
                     /protein_id="HFM8613698.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            14033..14509
                     /gene="ybeY"
                     /locus_tag="ACHAZB_001008"
     CDS             14033..14509
                     /gene="ybeY"
                     /locus_tag="ACHAZB_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFM8613699.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            14493..14888
                     /locus_tag="ACHAZB_001009"
     CDS             14493..14888
                     /locus_tag="ACHAZB_001009"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFM8613700.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            14903..15802
                     /gene="era"
                     /locus_tag="ACHAZB_001010"
     CDS             14903..15802
                     /gene="era"
                     /locus_tag="ACHAZB_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFM8613701.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            15945..16757
                     /gene="recO"
                     /locus_tag="ACHAZB_001011"
     CDS             15945..16757
                     /gene="recO"
                     /locus_tag="ACHAZB_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFM8613702.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKSGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            17141..18058
                     /gene="glyQ"
                     /locus_tag="ACHAZB_001012"
     CDS             17141..18058
                     /gene="glyQ"
                     /locus_tag="ACHAZB_001012"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFM8613703.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENMKEAE"
     gene            18060..20135
                     /gene="glyS"
                     /locus_tag="ACHAZB_001013"
     CDS             18060..20135
                     /gene="glyS"
                     /locus_tag="ACHAZB_001013"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFM8613704.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKKSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
BASE COUNT         6761 a         3574 c         4381 g         5458 t
ORIGIN      
        1 tttgtaaatg ttactggaaa gaaacagatt tctattgttt gttttgttta caaaaaagat
       61 aatgaaagtg taaaaaagta aaggagtgag aacatggcaa aaggaatcaa aatcgctacg
      121 attggtggag gcagcagtta cacccccgag ttgatggaag gatttatcaa aagatatgag
      181 gaattaccga tacgagaaat ctggctcgta gatgtagaag aaggaaaaga aaagcttgag
      241 atcgttagtc agatgtctca gcgtatgtgg gatgcttctc cgtatgaagt caagatctat
      301 gcaactctgg atcgggaaga agcattgaaa gatgcggatt ttgttacgac tcagtttcgt
      361 gtcggtcaat tagatgcacg aatcaaagac gagcggatcc ctagttatta tgggatgatt
      421 gggcaagaaa caaatggtgc tggcgggata ttcaaagctt ttcgtaccgt tccagtcgtt
      481 ttggcaatcg ttgaggacat gaagcgttta tgtccaaatg cttggctgat caatttcgcg
      541 aacccaagtg ggatggtgac agaagcagtt atcagatatg gcaaatggga taaagtgatt
      601 ggattatgca atgtgccagt gatggcgatg atgacagaac cagcaatgct tggcaagaaa
      661 aaagaagagc tgatctacaa gtttgccggt ctaaatcatt tccattggca caaagtagca
      721 gataataaag gaaatgaccg cacggcagaa ttgattgatc gtttatacga tgaagagaat
      781 ggattaccga aaaatatctt tgatatcccg tttttcaaag aacaattaaa acaaatgaac
      841 atgatccctt gtggctacca ccgctattac tatcgtgaag aagaaatgct ggaacatgct
      901 ttagaagaat atcaaacgat tggtacaaga gcgcagcaag tgaaagaaac agaagcagag
      961 ctatttgaat tatacaaaga tccagaatta gaccataaac ctgaacaatt gcaaaaacga
     1021 ggtggcgctt attattcaga cgctgcctgt gaaacgattg cttcaatcta tggaaataaa
     1081 gaaacacaga tcgtggtatc tacaaagaat gatggagctg ttcctgacct gccggcagat
     1141 tgtgtagtag aagtcactgc ctatgttggc gcacaaggag cgagaaatgt agcgtttggt
     1201 tctcttcctg ctgctgaaag aggctggttg caagtaatga agaatatgga attgctgacg
     1261 atcgaagcag ctgtgactgg tgattatggg acagctttac aggcgtttac gatcaatcca
     1321 cttatacgat caggaaagac agcaaaacgg atcatggatg agttgtttat tgcacaccag
     1381 cagtatttac cgcaattcaa ggagacaatc agccgattaa aagaagaagg aatcgaagtt
     1441 caagatgaac tggcaagaga attatcaggt aatacacata taatggagga agtataatgg
     1501 gattcagaaa agatttttta tggggcggtg ctacagcagc caatcaatgt gagggcggtt
     1561 atgatgaagg tggacgcgga ctagcaaacg tagatgttgt gccaatcgga aaagaccgtt
     1621 ttcctgttat caccggaaag aaaaaaatgt ttgaatttga tgaggatcat ttttatccag
     1681 caaaaattgc aatcgatatg tatcatcgtt ataaagaaga tattgctttg tttgcagaaa
     1741 tggggttcaa gacttatcga atgtccatcg cctggagtcg tatttttcca atgggggatg
     1801 agaaagagcc gaatgaagct ggcttgaaat tttatgagga tgcctttaaa gaatgccaaa
     1861 agcacggaat tgagccactt gtgacgatta cgcattttga ttgtccaatg catttgatca
     1921 aagaatatgg tgcgtggcgt tccagagaac tcgttggttt ttatgaaaat ctgtgtcgag
     1981 tcatcttcaa tcgctataaa ggacttgtca aatattggct gacattcaat gagatcaata
     2041 tgatccttca tgcaccattc atgggcgcag ggctgtattt tgaaaaagga gaaaatgaag
     2101 aacaaatcaa atatcaagcg gcacatcatg agttggttgc aagtgccatc gcaacgaaga
     2161 ttgctcatga agtagatccg gagaacaaag taggctgtat gctggcagca ggagcatatt
     2221 atccatacac ttgcaatcct gaagatgttt gggcttctag aaaatcagat cgagaaaatt
     2281 atttcttcat tgatgtccag tcacgaggag aatatcccgc ttatgcattg aaagaaatgg
     2341 agcgaaatgg cattcagatc gaaatggaac caggggatgc cgcattgctg aaagaacata
     2401 ctgttgattt tatctctttt tcttattatg cttcccgagt ttcgactacc gacaacgaac
     2461 tcttggaaca gactgcagga aatatctttg cttctgtcaa aaatccatat ctgcaagcaa
     2521 gtgaatgggg gtggcaaatt gatccattag gcttgagaat aacgatgaat gatatttatg
     2581 atcgttatca aaaaccattg tttattgtag aaaacggctt aggtgcagtc gatacgccag
     2641 atgaaaatgg ttatgtggaa gatgattacc gaatcgatta cctggcagcg catatccagg
     2701 cgatgaagga tgcagtagaa ctagacggag tcgatctatt aggctatacg acttggggat
     2761 gtattgattt agtatcagct ggtaccggtg agatgaagaa aaggtacgga ttcatttatg
     2821 tcgatcgtga taaccaagga aatggcacat tagcccgttc aaagaaaaaa tcattcaact
     2881 ggtataaaaa agtaattgat tcaaatggag aaaatctttc gaacgattga tcaatgattt
     2941 ataaaagtct attgtttttc agcggatttt tcgagtataa taaagctatt cgagaaacgc
     3001 aagcagaaca atagactttt tttcgcattg gctgtaaagt atgcttactt gattaagtaa
     3061 aggagaatct acatatgtat gatgtaatcg ttattggcgc aggtcctgct gggatgactg
     3121 ctgctttata tgcttcacgt tcaaacttat ccgtattgat gatcgaacaa ggtgcccctg
     3181 gcggccaaat gaacaatacg gcagaaatcg aaaattatcc tggattcgat tcgatcatgg
     3241 gccccgatct agcttataag atgtatgaaa gtgtctcaag atttgggaca gaaaatgctt
     3301 atggaatcgt tcaagatatc aaggatcacg gcagccataa agaagtcatc tgtgaagata
     3361 aaagttacga aggaaaaaca gtcattatcg caacaggatg tatccaccgt aaattaggcg
     3421 tacctggcga ggaagaattt gctggacgag gcgtttctta ctgtgcagtc tgtgatggtg
     3481 cgtttttccg taacaaacgt ctagtagtca tcgggggtgg ggactcagca gtagaagaag
     3541 ccatctattt gacacaattt gcttctgaag tagtgatcgt ccaccgtaga gatgaattac
     3601 gtgcacaaaa gatcatccaa gaccgtgcat ttgcaaatga aaaaatttcc tttatgtggg
     3661 atacagtcgt tgacgaaatc gttggaaacg aaatggtagt gacaggtgtg aaagcacgta
     3721 atgtcaaaac cggcaatgaa tttgatttag cagcagacgg agtattcatc tatgttggac
     3781 tggatccatt aactgcacct tttagagaag caggtttgac gaacgaagct ggctggattc
     3841 caacagacaa cgaaatgaaa acgaatatgc caggcgtttt tgccatcgga gatgttcgtg
     3901 aaaaagattt gcgacaaatc actacagctg ttggtgacgg cggtattgct ggacaacaag
     3961 tgtataaata cttggagtct ttagaagaaa caacagcata agtttaaaaa gagtagaaga
     4021 acgggttcaa ttgttctttt actctttttt ttcgcgtgaa atttactgaa tctgatttat
     4081 gcgaacacgt ccaacacctt ttgataaaag aatcctttct tgatttaagc gttttttggt
     4141 cctcatactt tcatatctaa taagtgaatg ctatacttat taagaaccat atttcagaaa
     4201 gaggtgttat tttcatgtct tgggaacaag tgtatgaaca atgggtacat gaagaaaatt
     4261 tagaagaaaa cttaaaaaaa caattaaatg atctgggtga agatcctgaa aaacttgaag
     4321 atgcttttta tgcaccgttg gaattcggta ctgcaggtat gcggggaatc ttaggacctg
     4381 ggatcaatcg aatgaacatc tatacggtaa gacaagcaac agaaggacta gcacgtttca
     4441 tggatacaca aggtgaagaa acaaaacgcc gaggtgtcgc tatcgcttat gactcacgcc
     4501 atcagtcacc agaatttgca atggaagcag ccaaaacact agcacatcac aatatcccgt
     4561 cttatgtatt cgaaagtctg cgtccaacac cagagctttc ttttgcagtt cgtcatttga
     4621 agacgtttgc gggaatcatg gtaaccgcat ctcacaaccc agcggcttac aatggatata
     4681 aagtctacgg agaagatggc ggacaaatgc ctcctgcaga cgcagatgca ttgacgaaat
     4741 atgtccgtga agtcagcaat ccgctaaaag tcgaagtatt atctgacgaa gaagctaaac
     4801 atagcgggtt gatcacgatc atcggagaag aagtggacgc tgcttatcta gaagaaatca
     4861 aagcagtgac gattgatcgt gaattggttg aaacaatggg gaaagatttg aaacttgtgt
     4921 acacgccact tcatggaaca gggaaaatgt tgggcgaacg tgcgttgaaa caagctggat
     4981 tcgaacaatt cgtactagtt cccgaacaag ctgtggcaga tccagatttc actacggtca
     5041 aatcgccaaa tccagaagaa cattcagctt ttgaatatgc tatacgactt ggagaaaaag
     5101 aaggagctga cttgctgatt gcaacagatc ctgatgcaga ccgtttaggc gcagcggttc
     5161 gtttgccaga tggttcttat gaagtattga ctgggaacca aatcggagca ttgtttgctc
     5221 aatatatttt ggaagcacat caatcaagag gcactttacc agaaaacgca gcggtattga
     5281 aatcaatcgt ttccagcgaa ttgcctacag caattgcgga acactacggt gctaccatgt
     5341 tcaatgtgtt gacagggttt aaattcattg ctgaaaaaat ccaacaattt gaagaagacc
     5401 attcacatac ttttatgttt ggattcgaag aaagttatgg ttatttagtt aaaccttttg
     5461 ttcgcgataa agacgcgatc caagcattgc ttttattagc agaagtagca gcttactata
     5521 aaaaacaagg caaaacgttg tatgacggct tgcaaacgat ttttgaaacc tatggctatt
     5581 tcgcagaaaa aacgatttct gtgacaatga gcggacaaga aggttctgcg aaaatctctg
     5641 ctttgatgga aaaattccgt aaagaagcac cgacagaatt tgccggagca aaagttattc
     5701 aaacagaaga ctttaaagaa ttgacgagaa cagaccgtga aggaaacgta gaaaaactaa
     5761 caacaccgcc atccgacgta ttgaagtata cattggaaga aaatggctgg atcgctatcc
     5821 gcccttcagg aactgagccg aaaatcaagt tttatattgg tgtaaaagga agctctacag
     5881 aagacgcagc cgaaaaagta gcctcttatg aagcgattat caaaaagatg acagaagaat
     5941 aaaaaacttg agactgatgg aaaaagtggt aaactttaga agtcagtctt ttttcaagtt
     6001 ggaggtgaat ggattgccag acacgcaaaa atcaaaagta gagaaagtca gtcaattatt
     6061 caaaatgttg agtgatccca ctcgtcttaa aattctgcta tatctgaaag acggggaaca
     6121 aaatgtgaca gcaatcaccc aagcagtgga aatggagcag tctgctgtat cgcatcaact
     6181 acgtttatta agagaaaatc atgtcgtcaa gtctcatcgc gagggaaaag cgattttgta
     6241 tagtcttgat gattctcatg tactggatat tttgaaccag acactcaaac atgtggaaca
     6301 tcaataacga taggtgaaaa tagataaaca ctggagtgtg acaacgaaga aatttcgttg
     6361 tcacactcca gtgtttttta agaaaaagac aaaaatgatt aatcgctttt ttctgttgtt
     6421 tcagttccat aataactatg atcttctagg tctaaagcat tcaaaataag cgaagccgtt
     6481 tcttcaatag ataaagatgc cacattgatc actacacatc ctagtttttc ataaagatca
     6541 ttcgcaaaat cgagttcagc tctaattttt tcgatatcgg aataagcagt atcagggttt
     6601 aatccgtaag agcgcatccg ttctttacgg atattattta aaatatctgg gttattcgtt
     6661 agtcccacga tttttttagg atcgatttcc cacagctgtt tgggaatatg tgcttgggga
     6721 atcaaaggta aattagcaac ttttaaattt ttatttgcta aaaacaagct caaaggcgtt
     6781 ttggaagttc gggatacgcc tagcaaaacg acatctgctt ctaaaaaacc acgaggatct
     6841 ttcccatcat cgtattttac agcaaattcc attgctttga tccgtttgaa atagttctca
     6901 ttcaataaat ggagggcacc aggttgccga attggcttga taccagttaa acgttcaatt
     6961 tctgatatcg gtggcgtcat cacatcgaaa ctaaataatt gatgttcttc acaaaaagaa
     7021 cgtgttatct cgatcagttc ttgattgatt aatgtatgca ggacaagtgc tttttcttct
     7081 aatgcatctt gcaatgcctg ccttaatgtt ttttcatctg tcacgaacgt tctgcggaat
     7141 aggttgaact caacggtagg atactgagcc atagtagctt gcgctagttt tgtcgcagtt
     7201 tctccagcag agtcggagat gacaaaaaaa gtaacgattt ctgtagaatc ggaagacatg
     7261 gataaaacct cacttgcatt ttttttatgt ttattatacc atactctaga atgcgtactt
     7321 cgtaatcatt ttgatttgga ggaataagtc atgaaaaaac agacattgat cgaacaatca
     7381 ttagaaacat tgaaaaataa cggttttaaa tatactaaaa aaagagaagc attgttaact
     7441 tatttagtga agcgtaaccg ctacgtatct gctaaagaag tttttgacta tatgaatcag
     7501 gaattcaaag gggtaagtta tgatacgatt taccggaatc tccatgattt tgcagagatc
     7561 gatttactgg aagaaaccga tttaaatggt gaaatgaagt ttcgttttca ttgctgtcag
     7621 ggggaaatcg gccatcacca tcattttatc tgtacagtgt gcggaacaac gaaagaaatc
     7681 catatgtgtc caatgaatta tttcgaagaa cagctaggtg gatgcacgat cgaagggcat
     7741 agattcgaga ttttcggaag atgtgaaaat tgtcaataag gatttaaata aaataacatt
     7801 tgataattta aaaaaaaagc aagatttcct tgacggtcaa aacagcatta gatataatat
     7861 ttacaggaca gtcttgtttt ttgagatata tttcaataac aattacggtt actttttaag
     7921 ctcggaggga gggaattaac atgtcaaaaa cagtcgttcg caaaaacgaa tcacttgacg
     7981 atgctcttcg tcgcttcaaa cgttccgtgt caaaagcggg tactttacaa gaatctcgta
     8041 aacgtgaatt ctacgaaaaa ccaagtgtaa gacgcaagaa aaaatctgaa gcagctagaa
     8101 aacgcaaaaa attctagttc tgtgatgaat tggagttggt agtgtgtcac tactaagtag
     8161 attaaacgat gacatgaaag cagcgatgaa agcgaaagat aaggaatctt tacaagtcat
     8221 ccgaatgatc aagtcatcta tacaaaacga gcagattaaa gaaggtcacg acttgacaga
     8281 agaagaagaa ctaacggtgt tgtctcgcga aatgaaacag cgacgtgatt ctttgcatga
     8341 gtttgaagaa gcaggacgtg acgatttagc tgaaaaagta aaaagtgaaa ttgtaatagt
     8401 cgaaaaatat atgccggaac aactttccga cgaagaaatt cgacaacttg ttcaagaagc
     8461 aatcactcaa actggtgctt catcaatgaa agaatttggc aaagttatgg gtgcaattat
     8521 gcctaaagtc aaaggaaaag cagatggaaa ccaagtgaat gcgattgtga aagaactatt
     8581 acaatcttaa ttttatcatt taaactgcgg acagtgattg tccgcagttt tttgtgttcg
     8641 taaaaaaaga actaaaaaac agctagaaga aagaataaga ttctttcttc tagctgtttt
     8701 tttcctgttt aatatctctt atgttaaatt gtgtttgatt tggttattct tctggtagtt
     8761 catttgcttc ttgtttatac cctgataggc gataggcgta ataaaagagt aagctgccag
     8821 cgtaaccaag taacgttggg accatccagc caagtcctaa tgcagcaaaa ggaacgtgat
     8881 gatgataaaa tcctagtaag cttgctacga cagtttgctg actcactaat gcaggcgaat
     8941 ttgccaacat atccaatacg gctggaacgg cagaaaagaa catcgttact tgatagacaa
     9001 atggactctt ttggaagaat tttgctgtta aggaaagcaa gattaacgca agtgacaatg
     9061 gatacaaaag catcaatact ggtaaagacc attggatgat attatccaat ccggcattcg
     9121 caacgacaaa agagacaaaa gtcgttaacc gcagccatgt catataactg actttaggga
     9181 acagtttatg gaaatcttgt gcaaaagaaa caacgagtcc catagcggta gtgaagacac
     9241 ctaatgtgac cattacccct aagaagctag caccgaagtt acctaaataa aaattagtga
     9301 tctgagaaag tgcagttccg ccattttctg ataaaggcat cttattcaaa ctcatcgcac
     9361 cgagaaggac cagaccaaaa taaatcatga cttctaacgc aacgctcagg ctaccagctt
     9421 ttgctgttaa ttctgtgact tttttatctt taaaaccaag atcacgaact gcatggacaa
     9481 aagtgacact taaagcaagt aaagctactg catcaaccgt gttgtatcct tctaaaacgc
     9541 catttgtcaa agcgttcgtc tgataagaat ctgcagcaga ttggtttaga ttccccatgg
     9601 gttggaataa agaaacgaca aaaatgatag ctaataatgc aagaaacaag gagtttaaat
     9661 actttccgac atatttgacc aaacgtgttg gatgtacagt aaggaaataa gcaagtaaaa
     9721 agaaaagagc agagaataaa aacataccta atgaatcata tttttcaggc aaaaagggtt
     9781 tagcagccat ttcaaaagca gttgctgctg tccgcggtgt accaaagaat ggtccgatcg
     9841 ttaaatgaac aaggatcaag aacaaggcag cataccattt tccgacaggt ttagctaagt
     9901 catacagacc atcacttttt gtcactacta cagctaagat agctaataat gggaaaagag
     9961 cgcctgagat ggcaaatcct acaccagcgc tgatccagtt tgatccagcc atttgaccta
    10021 agtggacagg aaagatcaga ttacctgacc cgaagaacat cccgaagata agtgaactga
    10081 ccatcaataa ttgcttccat gaataagttt ttgtttcttc cataaatttt tcctccaata
    10141 attatcattt tgagtaacaa aataaacgtt gcagaaacta gaaatgccac taatatcaga
    10201 ttccatgaaa accaccacct taaaatataa aaaaaagccc ttttccactt gggaaaaggg
    10261 ctctagattc tagaacggta ccaccttttt tcgcttaaaa aagcttcttc attcacggac
    10321 gcttttcgat aaagttatcc gctagcctga taatggaggc agccacaatg atctacgttt
    10381 tcgacattga ctcggaaatg atgttcgtct attcgattta ttgtcatttc tcacttacat
    10441 gactcactgg gaataaatcg ctgaatagcc tactgttttc ctcgacgctt tgctgtatat
    10501 ttatttgttg acgttataat acacctgttt tttttgaaat gcaagaatat ttcaaatatt
    10561 ttgttttctt tttttgaaac aagaaaaata cccagagaat acttgctaag ttagacggaa
    10621 tacttgtcct ctatcttatg catagcttac atgatcagct ttttttattc aaacgagacc
    10681 tttagagaat ggaagcagct attttttaac gtttcttggt acttatggtt aagcaactct
    10741 tttagaacct tttcttattt gctatatagt ttcttctgag tatggtaaaa taggaaagaa
    10801 tgaatcgaaa aaaaggggat gtctattttt gacggaagag tctagttctt tagagattag
    10861 attgacggct gctgatgatg tcagcatgct tttaggtgca cacgacaagc atataaaact
    10921 gattgaagaa acaacagaaa caaccatcca tacacgtgga gaaatcattc agatcattgg
    10981 cgaacaatcc cagatttcct tggctgcctc agtcattcgg acattgcagg aattgatcaa
    11041 acgaggaatc catgtaagta caccagatgt cgtgacggca ctgaaaatgg ggcaaaaagg
    11101 cacattagat tattttgttg aaatgtatga ggaagagatc gtcaaagacc gtaatggaaa
    11161 accaatacgg gtaaaaaaca gcggccaaaa gaaatatgtc gaatcggtag ccaaacatga
    11221 tatcgtattt ggcgtaggac cagccgggac aggaaaaact tttcttgcag tagtattagc
    11281 aatcgctgcc ttgaaaaaag gtgaagtgca aaaaatcatt ttgacgcggc ctgctgtgga
    11341 agcaggggaa aatctaggct ttcttcccgg ggatctaaaa gaaaaagtag atccttatct
    11401 gcgtcctgtt tatgatgctt tataccaaat ttttggatta gatcatacga accgattgat
    11461 ggaacgaggc gtgatcgaaa ttgctccttt agcttacatg cgggggcgga cattggatga
    11521 tgcattcgtt attttggatg aagcacagaa cacgacagtt gctcagatga aaatgttcct
    11581 gacacgtctt ggttatcaat caaaaatgat tgtcaatgga gatacgagtc agattgatct
    11641 gcctcgaggg acaacaagtg gtcttgttca tgcagaacga acattaaagc aaatcaagaa
    11701 aatcgatttt gttaattttg aagcaagtga cgttgttcgg caccctgtag ttgccgaaat
    11761 catcaaagct tatgaaaagg cagatctcca tcaagaataa gctttgctta ggaggataag
    11821 cacatgttga ataaaacact gcagaaacta caagaaaaac ttggaaaggc agcattgcct
    11881 ttcctgctgt tcttgttttt tatcattatc tgcggcgtta catttagtag tgtcaaacaa
    11941 aaggccaatg atttcagaga aggccaagta gcagaagaaa gtatccgtgc gaacaaaacg
    12001 atcgagaaca cggaagaaac cgaacaaaaa agaaaactgg cagcagaagc agtcactcca
    12061 gaatacactt accaaaaaga tcttgcagat gaccagaaca atcggatcaa gcaactattt
    12121 gaattgatcg ataaaacgaa tgatgccatc aacaaatctt atgacgaaaa gattgataag
    12181 gcaaaggaag atgaaaatat cccaaagcct actacagaag agcggatcgc cgcgttgaag
    12241 aaaaattttg aaaatgtgaa tgcagaaaat gttgcgtttt atcaaaaact accgaacaat
    12301 ttttattcga cgatatttga aatgactgaa actgagatcc atactgtgag ggatgagagt
    12361 ctgaagctca ttgacgaaca gatgagcaaa caagttcgtg aaagtgaatt ggaagctttt
    12421 aaacaagaag cagaagagca aatccaatat ttgaatgtca caccagcaca acaacaaatg
    12481 attcgctact tagtggatca aggtatcgta gtgaatgacg tattgaatga gaaaaagaca
    12541 gaagaattga aacaatccgc acgagaagct gtacaaccag tgatgatctt ccaaggagaa
    12601 atcattgttc gtgaaggtaa tcaaatcgat gcttcagcta tgaaaaaatt ggaattacta
    12661 ggactgacta atcaaacaac ttcgattttt ccgctaattg ccatggtttt agctgttttg
    12721 cttcaaatcg gcgtactgat ttataattca cttcaattcc aaaatgcagg agaaagaaca
    12781 aaatacgtgc ttttctatgt gacagctatg tcagtcagca tagtgctgat gaaatttttc
    12841 caattattcc aaacggaaca agctacttat atcccgctgt tttatcccgc tgcttttgca
    12901 ccacttgttt taagcttttt cttgaaccga agagcaggga tcatggctgg tattttccaa
    12961 gcagtatctg cacttttcat tttttatgat tcgattggca cgaactttct aacgattatc
    13021 ttgatgtctt atgttttctc tgggctcatg gcaacgattg tcagacgcaa acggatctca
    13081 gaacaaggat tatctgctgc catgtgggtc attatttttc ctgtgatgat ggatgtcatt
    13141 ttgatcgtct atcaaggaat gagctttggt gatgcaacga cttggttaat gcttgtctgc
    13201 gggttcactg gtgcactctt ctcattcttg ctgacgatcg gtctacaccc atatattgaa
    13261 ttgatggtca atgatgacag tgtaattgta ttaaatgaac tcagcaatcc aaatcatcct
    13321 ttattgaaac agttgctgga agaagcgcca ggtacttatc atcatagtat gatggtagct
    13381 aatctaagtg ctaacgctgt agcagaaatc ggcggacgtt ctttgctaac tcgagtagct
    13441 tgctattatc atgatatcgg caagatcaaa catgctagct ttttcgttga gaacttgcct
    13501 tcaggagcgg aaaacccgca caatttctta ttgccagaag acagtaaaca tattatcttt
    13561 ggacatgtga cagatggagc caaaatattg gaagattata atatgccgca aatggtgatc
    13621 gatatttgca aacagcatca tggaaccacg ctaatgcgtt atttctacat caaagcgaaa
    13681 gaacgcaatc cggaagtaac agaagagcaa ttccgttatc ctggaccaaa accgcaaagt
    13741 cgtgaagcag ggatcgtcaa tattgctgac agttgtgagg ctgcagtacg ggcaatggat
    13801 catccgacga gcgaaaaaat cgaaagtttt gtccataatc ttatcgaaga gcgtatttcc
    13861 gatggtcaat tagacgatag tggactgacc ttgaaagaaa tccgaaaagt ggaaaaatca
    13921 ttgatcagcg gtttaagtag caccttccat tctcggatca aatatcctaa aatgaaaaca
    13981 gaagttgaga agataaagga agaacaagaa gaatagaaag aagcgatgta taatggatat
    14041 tacctttatg gatgaaacaa accaagtctc agaagagaaa atgaaagaaa tcgatgactt
    14101 gctgcaattt gcagcatctt atttaaagct gccagaggaa acagaaatgt ctgttacttt
    14161 catggacaat gcggcaatcc aagtaatcaa tcgtgattac cgtggaaaag atgcaccgac
    14221 agacgtcatc agttttgctc tggaagaaga aggcgaagac gaaatcccag tgatttttga
    14281 agatggagag aatccattgc ctcgtgtact aggagacatc atgatttcta ttgaacgtgc
    14341 tgctgaacaa gcagaagctt acggccattc atatgatcgg gaacttggct ttttagcggt
    14401 acatggattc ttgcatatca acgggtatga ccatatgaca ccagaagatg agaaagaaat
    14461 gtttggttta cagaaagaga tcttggatgc ctatggactc aaaagataaa aacgtagaga
    14521 aaaacaaaca tttcctgatg tctgtggaat ttgctgtcca aggagtcaaa accgtttttg
    14581 aggaagaacg caatatgagg aaacatgttg cgttcggtat ccttgcttta tttatgggcg
    14641 tgattttcca gcttgatcgg atagagtggt tatggctgct tttagctgtt tttctcgttt
    14701 ggatagtaga gatcatcaat actgtttttg aaaatgtagt ggacatgttt acggattttc
    14761 attttcatcc gattgggaaa aaaatcaaag atatggcagc tggagcagtt ttagtgaccg
    14821 cttgttttgc tgtcatcatt gggttgattt tatttgtccc taaaatatat catttgttgt
    14881 ttaattaaaa aggagaacgt atatgccaga acataaatca gggtttgtcg caatcgtcgg
    14941 ccgcccgaat gtaggaaaat ccacattatt gaaccgaatc gtgggtcaaa aaattgccat
    15001 tatgagtgac aaggcacaga caactagaaa taaaatccaa ggagtatata ctacaccaga
    15061 agctcagctg atttttatcg atacaccagg aatccataaa ccaaagcatc gtctaggaga
    15121 ttttatggta gaagcagctt acagtgcgtt gaaagaagtg gatgcagtct tatttatgat
    15181 cagtgctgat caaaaacgcg gccggggaga cgattttatt attgaacgtc taaaaaacgt
    15241 gcattctcca gtttatctga ttatcaataa aatcgataag atccatccag acgagttgct
    15301 gggaatcatc gaagattatt cgacgcagat gccttttgca caagtgattc cgatctctgc
    15361 tacagaagga aacaatgtcg aacgcttaat ggatgtatta gtcagtgaaa tgccagaggg
    15421 cccacaatat tttccagatg atcaagtgac tgatcatcct gaatacttta tcgtttctga
    15481 attgatccgt gagaaagtct tgtttttaac acgagacgaa gtgcctcatt ctgtcgcagt
    15541 cgttgttgat tcgatgaagc gaaacgagaa tgataaaatc catatccagg ccacgatcat
    15601 cgtcgaaaga gatagccaaa aaggaatcat tatcggcaaa ggcggaaaaa tgctgaagca
    15661 agtaggaacg aaagcaagaa aggatatcga aaatctttta ggagacaaag tatttctaga
    15721 actttgggtc aaagtccaaa aagactggcg agacaaaaaa gtctatctgc aagactttgg
    15781 ctaccgaaaa gacgaatact gatattctga acttcttata ggttaagtat agaaataaac
    15841 tggataaaaa tccgcacgtt gagaaaaacg gcggaagatc gaaacataaa taatctggat
    15901 cggcagaatc tgcttttcca gatttatttt ttaggaggga gaatatgagc cagcttgcag
    15961 aatcgaaagg cttgatttta tctagccgta attacaaaga aaaagataaa ttagtcaaaa
    16021 tctttacgga atcttctggc aaaatgatgt tttatatcaa aggaatccat cggcaaaatc
    16081 agccattagc tccggctttg ctccccttta ccgaagcagt ctatatcggt aattttcgaa
    16141 gtgaaggttt gtcgttttta agcagtgtca aagacgtcca tgcgtttcgg aacatccaag
    16201 aagatatctt tttgagcggt tatggtacct atttgctaaa tctagtagat gcagcaatcg
    16261 aagatcatca atatgacccg catttattcc aatttacgca acaagctctg cagcggatgg
    16321 atcaaggaga tgatgcagaa atcatcacga atatctttga agttcaaata ctgcaacgct
    16381 tcggaatcgc gccaatttgg acgcactgtg tcgtatgcgg ggaaacaaaa ggaaaatttg
    16441 actattcttc caaatctggc ggtgtgatat gcgagaaaca ctggccaatg gacgaacacc
    16501 gttatcatgc agatcctcgc gctgtttatt tcgttcgaat gttttcggct atttcttatg
    16561 ataagatcag tggaatccac ttgaaagaag aaacgaagca ggcaatccgt gacctcattg
    16621 accagttgta tgatgaatat gtcggtattc atctaaaaag caaaaagttc attgatcaaa
    16681 tgaagtcttg ggcacacgta ttaaagacgc cagagaaaaa tatgcctgaa aaagagatac
    16741 ctgaaaaaga agaataaatg aaagatacaa caaaaaaatc aaaactattt gacaaataca
    16801 gggacttccg tctattatta accgtagatt aaatagatcg ttcactgaga ggatctatta
    16861 tataaacaag aaaactgcaa aatgaatacg tacattgacc aagatcagta aaaaacacgt
    16921 ggtttagcga gttcgggaga gtggaagccg gacccaaacg atttttgaag aggcacttgc
    16981 gagtacgata aatgaagctg ccgaatcttt attcggaagt agggtggaac cgcgaaatta
    17041 ttcgtcccta tgtctgtaat cagacatagg gactttttgt cgttttcttt cctgtattct
    17101 tattgacagc aacgcttaaa aattgaaagg ggcaaaacag atgagtaaaa aattgaccgt
    17161 ccaagatatg attttaacat tacaaaaatt ttggtcatca aacggctgca tgctgatgca
    17221 agcttatgac acagaaaaag gagcaggtac gatgagccca tatacattct tacgggcaat
    17281 cggaccggaa ccatggaatg ctgcttatgt agagccatcc cgccgaccag ctgacgggcg
    17341 ttatggagaa aacccaaacc gtttgtatca gcatcatcaa tttcaagtcg taatgaaacc
    17401 ttcacctgaa aatatccaag agctttattt agaaagcttg cgtcttttag gaattgatcc
    17461 attagaacac gatattcgtt ttgttgaaga caactgggag aacccatcga tggggtgtgc
    17521 cggtgtgggt tgggaagtat ggcttgatgg gatggagatc acacaattca cgtacttcca
    17581 gcaagtcgga ggactacctt gtaaaccagt tacttctgag atcacttatg gactagaacg
    17641 tcttgcttca tatattcaag aagttgaaag tgtctatgat ctggaatggg ctgatggtgt
    17701 gaaatatggc gagatcttca aacaaccaga atatgaacat tccaaatatt cttttgaagt
    17761 aagtgaccaa gacttgctac taagcaactt tgaccgtttt gaaaaagaag ctaaacgctg
    17821 tatcgatgaa aatcttgttc atccagcgta tgattatatt ttgaaatgca gccatacatt
    17881 caatttatta gatgctcgcg gtgcagtatc cgtaacagaa cgagcaggtt atctttcccg
    17941 tatccgcaac atggctcgtg ctgtagccaa aatctttgtg gcagaacgcg aaaaattagg
    18001 attcccttta ttgaataaaa acacagcaaa tccagaaaac atgaaggagg ctgaataaga
    18061 tggcaaaaga cttattactt gaaatcgggc tggaagaaat gcctgctcat gttgtcacac
    18121 caagcatgaa acaattagaa gaaaaaacag caaaattttt agatgaaaac caattgactt
    18181 atgatacgat cgaaacgttt tctacaccgc gccgcttagc aatcaaagta acaaacattc
    18241 cagaacgtca agcagacagt gaagaagaag tcaaaggacc agccaaaaaa attgctttgg
    18301 acgcagatgg aaactggacg aaagcagctg aaggatttgt ccgcggacaa ggtctgacga
    18361 cagatgctat cacttttaga gaattaaaag gcgtggaata tgtctatgta acgaaataca
    18421 ctcacggcaa agaagcgaaa gaagtattga caggtttagc agatgtcatt aaaagcctca
    18481 ctttcccagt aacgatgcat tgggcagaat acgattttga atatatccgc cctattcact
    18541 ggatcgtggc attgttagat gacgaagtaa tcccgtttga gatcttagat gtcaaaacag
    18601 gcagagcctc tcgaggacat cgtttcttag gcgaagatgt gacatttgcg catgcaaaag
    18661 aatatgaaga aaaattgaat gaacaattcg ttattgcttc accgcaaaaa agacaagaaa
    18721 tgatcgttac acaagcaaat gaattagccg aaaaaaatca ctgggttatt gatctagacg
    18781 aagatttact agaagaagtc aacaatttag tcgaatatcc aactgtcttt tctggcgact
    18841 tcgaagaaaa atatctttct gttcctgaag aagtactagt cacttctatg aaagaacatc
    18901 aacgttattt cgaggtacgg aacgatcaag gtatgctgct tcctcatttt atttctgctc
    18961 gtaatggtga tcgggtaaaa ttggaaaatg tggttcgagg caatcaaaaa gtattgacgg
    19021 ctcgtttaga agatgcggaa ttcttttata atgaagacaa gaaagcttca atcgaccaat
    19081 acgttgaaaa actaaaaact gttactttcc atgaaaaaat cggtagtatc tatgagaaaa
    19141 tgcagcgggt acaagtcatt gcccgtatca ttggcgaaca agtaggacta tctgaggaag
    19201 aattgatgga tctgcaacga gctgctgaaa tctataaatt tgatctagta accaacatgg
    19261 taggggaatt tcctgaacta caagggatca tgggcgaaaa atatgctctg ctgaaaggcg
    19321 agaaacctgc tgtagcaaca gcgattcgtg agcattacat gcctatatcc agtgaaggag
    19381 agctacctca gtctacagta ggttctgttt tagcgattgc agataaatta gatagcattt
    19441 tttctttctt tgcagttggg atgattccaa gcgggtcaaa cgatccttat gccttacgtc
    19501 gccaagcata cggtgtcgtt cggatcatcg aatcgaaaaa atgggccttt cctttgtctg
    19561 ttctgcaaga aacgattagt gatgttatca gtaaagatac ggatcgtttc ggcattggtt
    19621 tatcagaagg gcaacaacag gtgatcgact ttatcaaagg ccgtctgcgc caattattga
    19681 caacgaagaa tattcgtcat gatgtcatcg aagctgtttt gaatgcagaa caaaaagatc
    19741 tgacaaaagt atttgcagca gcacaattgt tcaagcagca tcttgcagat gaagacttca
    19801 aaccatcgat ggaagctttg acacgtgtcg tgaacttagc gaaaaaagcg gagcttgaaa
    19861 aacaatcaga agtagaccct gaactgttcg aaaatgaagc ggagaaagaa ctgcacaaag
    19921 cagtagaggc aatgaaaaaa agctttgctg accaaacgat caacgaaaat tatgatcagc
    19981 ttgtaagcct tcgcccactg atcgaaaatt attttgatca gacgatggtc atggcagaag
    20041 aaccagcagt acgtaagaac cgattaacgc aattaagtca aattgctgcc atggctttgt
    20101 cgctagcaag tttagatcaa atcatcacaa aataatttct aatatgtaaa taaaccagag
    20161 aataggcaca aaat
//
LOCUS       DAYYCY010000034        20044 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000034 DAYYCY010000000
VERSION     DAYYCY010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20044
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.034"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..3366
                     /locus_tag="ACHAZB_001014"
     CDS             <1..3366
                     /locus_tag="ACHAZB_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFM8613705.1"
                     /translation="KVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAEVISDQSGK
                     KALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITLEISSNTKQ
                     TIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIEEESSKENE
                     NSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVLDHQGNKNG
                     TNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDVYLNARGNV
                     QKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINMGYVGYSSE
                     GYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPNGHKKVIVL
                     LTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQNNLSRRID
                     STFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSSDEKGDLYY
                     ESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVGTSPTTVTP
                     SISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTTFQPNIDTN
                     ELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAAAWKNGYIR
                     IIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIKELPVPGYD
                     SQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLTDHGDGTYS
                     LPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVTTSDDTIQL
                     TIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQTTNEKGLAS
                     FDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNVWTLTHQNR
                     LKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLKPGTYTLTE
                     TFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITNQAKVPLPE
                     TGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            3369..4790
                     /locus_tag="ACHAZB_001015"
     CDS             3369..4790
                     /locus_tag="ACHAZB_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFM8613706.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            4787..6664
                     /locus_tag="ACHAZB_001016"
     CDS             4787..6664
                     /locus_tag="ACHAZB_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFM8613707.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            6756..7598
                     /locus_tag="ACHAZB_001017"
     CDS             6756..7598
                     /locus_tag="ACHAZB_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFM8613708.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(7750..8625)
                     /locus_tag="ACHAZB_001018"
     CDS             complement(7750..8625)
                     /locus_tag="ACHAZB_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM8613709.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            8832..9863
                     /locus_tag="ACHAZB_001019"
     CDS             8832..9863
                     /locus_tag="ACHAZB_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8613710.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            9952..10257
                     /locus_tag="ACHAZB_001020"
     CDS             9952..10257
                     /locus_tag="ACHAZB_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HFM8613711.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            10436..12451
                     /locus_tag="ACHAZB_001021"
     CDS             10436..12451
                     /locus_tag="ACHAZB_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM8613712.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            12453..12737
                     /locus_tag="ACHAZB_001022"
     CDS             12453..12737
                     /locus_tag="ACHAZB_001022"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8613713.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            12763..14136
                     /locus_tag="ACHAZB_001023"
     CDS             12763..14136
                     /locus_tag="ACHAZB_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFM8613714.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(14219..16234)
                     /locus_tag="ACHAZB_001024"
     CDS             complement(14219..16234)
                     /locus_tag="ACHAZB_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HFM8613715.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(16256..17470)
                     /locus_tag="ACHAZB_001025"
     CDS             complement(16256..17470)
                     /locus_tag="ACHAZB_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8613716.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            17690..18634
                     /locus_tag="ACHAZB_001026"
     CDS             17690..18634
                     /locus_tag="ACHAZB_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFM8613717.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            18930..19874
                     /locus_tag="ACHAZB_001027"
     CDS             18930..19874
                     /locus_tag="ACHAZB_001027"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFM8613718.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
BASE COUNT         6828 a         3689 c         3828 g         5699 t
ORIGIN      
        1 aaagttattt tcatgctgat aatattgagt ttatcaaact ttgtgccatt atctgcaata
       61 gcagacacta cagatgatcc aacagtttta gaaacaattt cagctgaagt catttcggat
      121 cagtctggaa aaaaagcact gaacatcaag ctaaatgcga ataacaccag tgctgaaaag
      181 atagaaaaag aaattggtct agtcgaaaat tacttaagtg atgtggaaag aaaagaagga
      241 gatggctatg cttatcaggt aaatagcggg aaaattacgt tggaaatctc atcaaacact
      301 aaacaaacta tcgatctgag ttttccaatc gatccagcac tttaccacag ccaggcaaac
      361 aagctgatcg tcgataataa agaatatgac attattgatg agacagaaaa taagaaagat
      421 acagatgtgt cagtaccaaa gccagacgaa atagaagaag aatcatcaaa agaaaacgaa
      481 aattctgtca gcccatttac attgcctaca ttatccttgc cagctgtgag tgtgccatct
      541 aatcaaacga ttcctacaga atatacaaca gatgatcagg gcacttatcc taaagccagt
      601 tggcaaccta caggaaatac aaatgttctt gatcatcaag gcaataaaaa cggaacaaat
      661 caatgggatg gtataaattc ttggaatgga gatcctaatg atcggaccca ttcgtatatc
      721 gaatatggag gaaccggtaa tcaagcagac tatgcgatac gaaagtatgc aaaggaaaca
      781 agtacacccg gattgtttga tgtttatttg aatgctcgtg gaaatgtaca aaaagatatc
      841 acgcctcttg atctcgtatt ggtcgtagac tggtcaggaa gtatgaacga caataatcgg
      901 atcggtgaag taaagattgg tgtcgatcgt tttgtcgata ctttagcaga tagcggtatc
      961 acagacaaaa tcaatatggg atatgtcggc tactcaagcg aaggatatag ctacagtaac
     1021 ggtgcagtac agatgggttc atttgattca gtgaaaaatc aagtaaaatc cattacacct
     1081 tcacggacaa atggtggtac ttttacacaa aaagcactaa gagatgcagg aagcatgcta
     1141 tccgttccaa atggacataa aaaagtgatc gttttgctga cggatggtgt accaacattt
     1201 tcctataaag tacagcgggt acacgcacaa tcaagcagca attattacgg aactcagttt
     1261 tctaatacgc aagatcggcc gggaaatact tctctaatct caagaatcta tgatgcacct
     1321 gaccaaaaca atctatccag aagaatcgac agtacgttta tcgcaaccat cggagaagcg
     1381 atggcactca aagaacgagg aatcgaaata catggtcttg gcatccaact tcaaagcgat
     1441 ccggcagctg gtctctcaaa agcagaagta gagtctcgta tgcgacaaat ggtttcatca
     1501 gatgaaaaag gcgatcttta ctatgaatca gctgatcatg caacagatat ctctgaatac
     1561 ctagccaaaa aagctgtaca gatctcagca actgtaagca atggacaaat aaatgatcca
     1621 atcgcagaac cattcattta tcagcctggt acactttcag tcaagagtgt ggggacaagt
     1681 cctacaacgg tcactccatc tatttccata gaaggaaata ccatcaagag caatcagatc
     1741 tatttaggaa aagaccaaga aatccaaatc cattaccaag tgagaatcca aacagaaaat
     1801 gaggacttcc atccaaattt ctggtatcaa atgaacggca ggacaacttt ccagccaaac
     1861 attgatacca atgaattagc tgaattcggt ataccatctg ctaaagctcc cggagtcagt
     1921 cttcacatca aaaagttatg ggaagaattt gacaacaatc tagctgatcg tccagatcaa
     1981 gttacttttg agattcaacg ggaacatacg acaaatgctg cagcttggaa aaacggatat
     2041 attcgaatca ttaaaccagc taaagataca acaaatacgt gggaacgtgc agacattgac
     2101 aaattatctg caaatagcgg agaaagttat caagagatat tatcactacc tcaatacaat
     2161 aatcaaggtc aagcattcag ttaccaaaca atcaaagaat tacctgtacc aggatacgat
     2221 tctcaacaaa tagatgcaat gacatggaaa aatactaaac aattcacacc gttaaacttg
     2281 aaaataacga aaaattcctc tacaggtgaa aaggatctta ttggcgctgt tttcaaatta
     2341 acaggagatt ctattgatac tttactaaca gatcatggcg acggaaccta ttctcttcca
     2401 gaaaatgtca aattgcaaaa agaaatgacc tatacgctga cagaaacaaa agctccagaa
     2461 gggcatggat taagcaaaaa gactacttgg gaaatcaaga tcgcttctga tggtacggta
     2521 accattgatg gaaaaacagt cactacttcc gatgatacga tccagttgac tattgaaaat
     2581 ccttttgttg aagttcctgt agcagtacgt aagtatgcga tgcaagggac ggacaaagag
     2641 ataaatctta aaggagcagc attttcccta cagaaaaaag aagcaaatgg tacttatcag
     2701 ccaattgaca gccaaacaac gaatgaaaaa ggtcttgcca gttttgattc actcacacct
     2761 ggtaaatatc gagtcgttga aacagctggt cctgccggat atgatacttc gccgggaaat
     2821 tatgaattcc aaatcgataa atatggaaaa atcatttaca cgggaaaaaa taccgagatg
     2881 acaaataatg tatggacgct cactcatcaa aatcgactaa aagcgtttga tctaacggta
     2941 cacaaaaaag aagacaacgg acagacatta aaaggagcaa aattcagact gcagggacca
     3001 gaaatggact tagaatcgcc aaaagatgga caagaaacag atacctttct attcgaaaat
     3061 ttaaaacctg gaacttatac gctgaccgaa acttttacac cagaaggata ccaaggtcta
     3121 aaagagccag ttactatagt tatacacgaa gatgggtcaa ttcaagtgga tggacaagat
     3181 catgaatctg ttctgtcacc aggagccaaa aacaaccaga tttctttaga catcacgaat
     3241 caggcaaaag taccattacc tgaaacggga ggaattggcc gtttaggaat ctatctagta
     3301 gggatgattg gttgtgcgtt ttctatttgg tatctttttt tgaaaaaaga aagagggggc
     3361 agctaaatat gaaaaaactt ggttggctta gtatgtgtct cttcttgtta ctatttaaac
     3421 cagcttttac tcaggtagca acagaaacag aaacagaaat ggttcagatt actttacaca
     3481 aattgctttt cccaaacggg caactgccga aaaatcatcc aaatgacgga caagaaaaag
     3541 ctttattaca aacgtatcga ggattaaatg gtgtcacatt ccaagtttat gatgtcacag
     3601 attcttttta ccatctacgg gaaaagggca aaacggtaga agaagcacaa gcagagatcg
     3661 caaaaaacgg tgcgtcttcc ggtatgttta ccgcagaagc aacaactaca actcttaaca
     3721 acgaagatgg tatcgcttct ttttctctgg ccgctaaaga tcaagaaaaa agagataaag
     3781 cgtatctttt cattgaatcc aaagtaccag aagtcgtcaa agaaaaggca gagaatatgg
     3841 tagttgttct tcctgtacat ggacaaaaca atcaaaaact ttcaactatc catttgtatc
     3901 ctaaaaatga agaaaacgac taccctgatc caccttttga gaaggtatta gaagagccta
     3961 gaaatgattt tacgattggt gaaaaaatca cttattcctt gcatacgaca attcctgtaa
     4021 atatccttga ctatcaaaag ttcgaattgt cagatagtgc ggatgaagca ttaacgtttt
     4081 tacctaatag tttaacgatt tcatcgaatg gagaaaagct gacagaaggc tttgtcatac
     4141 acaagaaacc tcacggattt gatgttttat tttcgatccc ttcgttggaa aaatatgctg
     4201 gaaaaaaact gaccatttct tatcagatgc agctaagcag tacagcacag gcgaacaagg
     4261 aaatcaacaa caacggaaca ctggattttg gttttggtgt cagtacaaag aaagtctctg
     4321 tatatacagg gagtaagcaa tttgtcaaaa tcgagacaaa taaaccagat aaacgattag
     4381 ctggcgcagt attccttatt aaaaacaaag caggaaatta cctccagcaa acagccaacg
     4441 gatacaagtg gacaaagaac gaatcagatg cgcttcacct gatttccgat aaaaatggcg
     4501 ctttttcaat ttccgggttg aaaacaggaa gttatcgatt aaaagagatc gaagcacctt
     4561 ctggttatat tttaagtgaa acagaaattc cgtttaccat ttcaactttt ctttctgagg
     4621 ataaagaggc ggacagtata ttgaaagtag tcaataaaaa agaaaatagc cgtccatttc
     4681 ttccaaaaac aaacgaaacg aaaaatacac ttttaggcgt tgttggtatg gtattcgcaa
     4741 gctttgcaat ctggttgttt atcaaaaaaa gaacaggagt gaaaaaatga aaaatcataa
     4801 aaaaataaac gttatgttag gagtcctttt ccttatttta ccattactca caaacagctt
     4861 cggcgcaaaa aaagtgtttg cagaggagac agcagctcaa gtcatccttc ataaaaagaa
     4921 aatgactgat ttacccgatc ctttaatcca aaacagcggg aaagaaatga gcgaattcga
     4981 tcaataccaa ggattagccg atatttcatt ttcagtttat aacgtcactc aagaatttta
     5041 tgcgcaacga gataaaggag cgtccgtgga tgcagcaaaa caagcagtcc agtctttgac
     5101 tcctggtaca ccagttgctt caggaacgac agatgctgat ggaaatgtca ctttatcttt
     5161 acctaaaaaa caaaatggga aagatgcagt ctacacgatc aaagaagaac caaaagacgg
     5221 agtgtcagct gccgcaaaca tggttttagc tttccctgta tatgagatga tcaaacaagc
     5281 agatggctct tataaatacg ggacagaaga actagatact atccatctct accctaaaaa
     5341 tacagtcggt aatgatggaa cgttgaaagt tacaaaaatc ggtactgccg aaaacgaagc
     5401 actaaatgga gcagaattta ttatttctaa agaagaagga acaccaagcg tcaaaaaata
     5461 catccaaagt gtcacagatg gattgtacac ttggacaact gatcaaacca aagccaaaca
     5521 tttcattact ggtcattctt atgacatcgg caacaatgac tttgccgagg catctattga
     5581 aaaaggccag ttgatcgtta atcatttaga agttggaaaa tataatttag aagaagtaaa
     5641 agctcctgat aatgcggaaa tgattgaaaa gcaaacaatc acgccttttg agatcctggc
     5701 aaatagccaa acaccagtag aaaagaccat caaaaatgat acgtctaaag ttgataaaac
     5761 aacacctcaa ttgaatggaa aagatgtcgc aatcggtgaa aaaattcaat atgagatttc
     5821 tgtcaatatc ccattaggta tcgctgataa agaaggaacg caaaacaagt acacaacatt
     5881 caaacttatc gatactcatg acgctgcttt aacatttgat aatgattctt caggaacgta
     5941 tgcttatgcc ttatatgatg gaaataaaga aatcgaccca gtaaattatt ctgtcactga
     6001 gcaaacagac ggattcacgg tttcagttga tccgaattat attccttcat taactcctgg
     6061 cggtacattg aaattcgttt actatatgca tttgaacgaa aaagcagatc caaccaaagg
     6121 attttctaac caagcaaatg tcgataacgg gcatacaaat gatcaaacac caccgtcagt
     6181 cgatgtcgtt actgggggca aacgatttgt taaagtagat ggtgacgtta catcagacca
     6241 aacacttgct ggagcagaat tcgtcgttcg tgatcaagat agtgacacag cgaaatattt
     6301 atcgatcgac ccatccacaa aagccgtcag ctgggtatcg gcgaaagaat cagcaacggt
     6361 ttttacaacc acaagtaacg gtttaatcga tgtgacaggt ctaaaatatg gcacgtacta
     6421 tctggaagaa acgaaagcgc cagaaaaata tgttccatta acaaaccgtg tagcatttac
     6481 tatcgatgaa caatcttatg taacagcagg acagttgatt tctcctgaaa aaataccaaa
     6541 taaacacaaa ggtacacttc cttcaacagg cggtaaggga atctatgtgt atatcggtgc
     6601 aggagtagtc cttctactga ttgctggact gtactttgct agacgcaagc acagtcagat
     6661 ttagtctttt tgcaagaatt ggaaaaagga gcagcggatt acgcaaatgc tcctttttct
     6721 tttattatca aacagtcatc acaaggagaa cacaaatgaa accaaagaaa aggaaacttc
     6781 tagatctgct gatgcttttc ttgcttctct caggaatagg gatactagct tatccatttg
     6841 ttagcgacgc gctgaataat tatctggatc agcagatcat ctctcattat cagcaacaag
     6901 cggtcaaaga aaatgaagaa gtcatggcta aaatccaaga aaacatgaca aagaaaaacc
     6961 agcaattagc aaaagaaggt ggaaatccag gtgcagatcc ctttactaag aaaaaagaac
     7021 ctgcgaaaaa ggatagaacc tattttgaga aacatacgat cggtattttg acgatccctg
     7081 agatcaatgt ccatttgcca atttttgatg aaacgaatac tgtgctgcta gaaaaaggtg
     7141 cctcattatt ggaaggaacg tcttatccta cggggggaga aaatacacat gccgttattt
     7201 ccagccatcg aggactgcct caagcaaagt tgttcacaga cttaccgaaa ctgactttag
     7261 gtgattcttt ctatatcgaa atcaatggac ggacactcgc ttatcaagtc gatcagatca
     7321 agacggtaga accgactgat acaaaagatc tgagaatcga aaaagggcag gatttcgtga
     7381 cactcctaac ttgtacacct tatatggtca acagtcatcg cttacttgtc agagggcacc
     7441 gggttcctta tcatgcaaaa gaagttcaaa aagaagtaca gaaagtatcc gagaacaaac
     7501 gatttttcct gtatggattg ttagctgcat gtatactgat tttcctcctt cttatatatc
     7561 tttttcgtca atcgttcaaa ataagaaaca gaagttagct attcctcatt tctataatat
     7621 atctattcga atcgttccgt aaacaaacag gctgaggttt gaaaaagaaa acttcagcct
     7681 gtttttatga tgatatcaaa gagagcctat caaacactat ctttcttatt actaaatgag
     7741 gatcttttct taagttttcc gatattcttt aagaaggcct ttttgcatca attcttgttt
     7801 gtttttcaag ttgcggatat tctcttcaaa attttgagaa tatatcactg agaataacgt
     7861 atttaatacg taatcgaagg caatttgaga ggagaaagtc gctactttag caaaatcata
     7921 ttcctcctgc gatataacaa gacacatatc agcctgtttg gccatttctg aatgctgatt
     7981 tccggtaatc agcaaggtag gggcctctac atgcttcaaa taggtcatta ttcgttcata
     8041 atgatggatg cgaccgctgt agctaataaa aatggcgcaa tcctcgctag tcaaattggc
     8101 tgcattccaa gaagaatcac tgtattcttc agccatgatc aaaaacttat tcaacttgac
     8161 cattttattc tggaatttcc ttgccgtgat ttgagaatca ccttttgcaa aaagaaagat
     8221 tcgctgtgcc ttgttcaata ctttaccgat ttgatctaaa tcttcatgtt caatctgcaa
     8281 ttgtgctttt tttattgact ggatcgataa atccgccatt ttctttgcaa tttgctgagt
     8341 gctatcttca cttgaaaaag gaaaattagg atcaatcgtt tgattgaact gagcaatcaa
     8401 ttgatgaact tcccgcccca gctcgaaacg gaattcttta aatccgctga atcccatttt
     8461 tttgcataaa cgaatgatag ctgaatggga agtatacgta gcttttgcta attcttcaat
     8521 cgtcatgtag actgcagctt cgatattcct acggatgtaa tcgacaatcc gtttctctgt
     8581 cgtggtaaac aactcttgtt ttgttagttt ttcttcaata agcatgatct gacctccaaa
     8641 ttctatctta ctgataaaca cttggttccg ctactatctt tttgattttt atccttactt
     8701 tgaacattaa tttcaccaaa ccgaatgata acaagtgttt ttctgaactt ttttaccaga
     8761 atagctatta taaatacttt ctgcgctttt ctaggatata ctgaaaacat caataaaagg
     8821 aggcaccact catgcttaca atcgcttatc ttggcaatgg aaagagtaca aatcgctatc
     8881 atttgccttt ttcaagaaaa cttacgaaca aaatcaatat caaaaaaatt ttttccccat
     8941 ctgaaccagt gtggaaaaca ttcgaagaca tcgaatatac tgatcagctg tctgatcttt
     9001 gggatgatcc tgagatccaa ttagttgtca tcactacacc tagtcaattt cattatgaat
     9061 atggaaaaat ggcactcgaa catggtaaga atgtactagt agagaaacct tttgcagaaa
     9121 cgtcaaagga agcagaagag ttatttgcat tagcaaaaga aaaaggtcta tttatccaat
     9181 gttaccaaaa cagacggttc gattctgact tcttgacagt ccaaaaagta atcgaaagcg
     9241 gaaaattagg ggatatttta gaagtagaaa tgcattatga ttatttcaga ccggaagtac
     9301 cagaaaatac gcatgaattt tctactgcga acagctattt atatggacat gcttgtcata
     9361 cagtagatca agttctttct tatttcggcg aaccagatga tatcagctat gatgtacgtc
     9421 aacttttagg gaaaggacga atgaatgatt acttcgatct ggattttttc tatggaaata
     9481 tgaaaatttc gataaaatcc agttatttcc gcatcaaaga acgtccaagt ttcgtcgttt
     9541 acggaaaaaa agggatgttc gtgaaacaaa caaaagatcg acaagaagaa catctgaaaa
     9601 tgttttacat gcctgatcat gaagactttg gtatcgattt gcccgaacat tatggtgtat
     9661 tgacctatat agatgaacaa gggagttatc atgaagaaaa agtaatttcc gaggttggtg
     9721 attacagccg tgtctacgaa gggatttacg agacattagt taatggcgca gaaaaagtca
     9781 tcaaagatga agaaacaatc ttacaaatga aaattttgga agaaggaatc aaaagcattc
     9841 aagaatcaga aggaaaaata tgacatattt gtaagaatcg ggtttgtttt ttgacgcata
     9901 aactgtctta ttgtacgatg aaaaagtata gaactactag gaggaaccaa catgacaaat
     9961 tatgacggtg aaaaacacaa acgattaaga ggttcaatgg cccctaaaac agaagaagaa
    10021 gtggctttag caggaactaa ttctggtgaa acagaagaca aagaagaatc attagctggc
    10081 acaaactctg gtgaaacaga aaataaagaa acagctttat ctggttcaaa ttctgtagaa
    10141 gaggaaacaa agcctaatga gcccaatcac tttgaaaaag atatcgaaaa agcggttgaa
    10201 gatgtcaaag aaaaatttga agaactgacc tcaaaaaata aacatgatca tcattgatcg
    10261 gacgaaatca attcataaaa gcaggagaca tttggtcacc tgcttttttt ctgtatccat
    10321 tcgatagaaa tcgttcggta cagtcgtaaa aaaaatccaa ctgtaagttg taccaaacac
    10381 ccctcgattt aaaagcgcta ccatactaga atataagtag gaggtgaaca gcgctgtgaa
    10441 ctacaaaatt agttatctcc tgaaaacacc aagagaaatc agtctagaaa aagcagcaga
    10501 gatcatcgaa gtaaatacaa gcgaggcgat caaacaatta actccttttg ccgaatatgt
    10561 ggatgatacc cgtatccgta taaagaaatt ccgagcaatc gactgggcag aaaaattttt
    10621 ggatattact cgatttgaag ctgtgtatac cgaacaagag cgaagagatc ttctttattt
    10681 gatggtttat tcgactgcaa cgtctgtttc tgtttttcat tttcaagatt ttcttcaggt
    10741 ttctaaaggg acagtcctgt cagatatcaa aaagataaga aatgaattag aagtggaaga
    10801 ttgtcagctg tcgtatacta ggaaaactgg ttttaggctg ctagggtcag aagcaatatt
    10861 aagaagagaa gcaaaaaatc gcactgctcg tttgatgcag tcacttgctg gaagatttgg
    10921 gctcacgtat ttaaccgcaa ccctttccat tgattattac gccaaagtta gagatttgct
    10981 agataaaacg atttcactat cagatcttcg attcgcacca aatcgaatgg atgaattagc
    11041 ttattatttg agtttgacaa ccattcgaat tggaaaaaca tccatggaac tggtaaaaga
    11101 tgctgaactg ttggcttctc ttgcagtcac tgacattagt cgacaagtat tgactggcct
    11161 tggctattcc gaacatcaaa attcggagat tatatttttc tctctttgct tagcatcgat
    11221 cgttgaagga gatgtccagg agcctgcact tgattttttg ctggaatgtt cttcagaagt
    11281 catccatcgc atggaattcc tgatggccgt agaatttgct tcgtttcgtg aattattgat
    11341 gaatgtcttt tatcacttgg tccctgccta ttttcgtatt tcctattctt tttatttacc
    11401 taatgtgatg atcgatcaaa ttaagcatca atatgcttcc atctacgaaa tgacaagaaa
    11461 agcattactt ccgttagaaa gacgaatagg gaagagcatc cctgaagaag agattggttt
    11521 tttcactata ctatttggcg gagagatcag aaaagcggac gcagaagaaa gaaatagaaa
    11581 aataagagcc gtgatcgtct gtccaagtgg cattagctct tcattgatct taaaaagtga
    11641 acttcagcaa ttattcccga tgatcttttt tacagagact aattcagctg accgcttaca
    11701 cgaggttgac gaaacttctt acgatattat tttttctact gtacctctca cacttactga
    11761 taagaaaaag tctctctatg ttctgcatcc aatcatgact gacttggaaa aaaatcagct
    11821 gatcaatcaa gtacaaagag attggctgat accaggattt tcaatgcctt ctgcccaaga
    11881 attgttgtcg gcgctactgc cttatatcga attaaaagaa ggcgtagacg aagaaaagct
    11941 ctatcaagta ttaaatcgta aaatgaacaa acttttagaa aagaaggagg atcaacgtcc
    12001 tatgctatct gaattattaa cagaagaaat gatccaatta tcggatcaac caaaaaactg
    12061 gcaagaagca atcacatttg ccgcacagcc gctattatca gaaaataaaa tagaagaaag
    12121 ttatatcaaa gctatgatcg agcgcgtgga gcagtatggt gcgtttatcc atattggcga
    12181 tcacatcgca ttacctcatg ctcgtccaga agatggcgta aacgaagtag ggatgtcttt
    12241 gctgaaactt caacaacctg tggatctgct tgataatcca gcccatccca ttagtttgtt
    12301 catctgttta gctgctatcg ataatgaggc acatcttcgg gctttggcaa atttaacaaa
    12361 gctactttct aataaacaaa atcttcaaga tttattacac gcatcaacaa aagaagaaat
    12421 attagcgatc ataagaaaag gagacgaatg atatgaaaat ttttgcagta tgtcaaagtg
    12481 gattaggaac aagttttatg gtagagatga acatcaaaca agcacttgca gatcttggag
    12541 cagatgtgga cgagtttgaa gtaagtcata cggatgttgg aagtgtatct agcgatatgg
    12601 ctgattattt cttcattgag cattctttga aagatacgct aacttctata cctgatgaaa
    12661 agctcgtacc tttgcaatcg atcattgaca gcgaagaagt aaaagaaaaa gtaacgaaga
    12721 tcttgtcaaa agaataaaaa tgattcaagg gaggaactat caatggataa attgttgaat
    12781 ttgtttgtgt caatcgtcag tcagccctcc atcctcgttt ctttgattgc actgcttggt
    12841 ctcgttcttc agaagaagaa attttcagaa gtgatccaag gaactatcaa aacatttgtc
    12901 ggattcctag tactgatcgg cggtgctggt ttaatcagta attcactaac cccttttgct
    12961 tcgatgttcc aaacagctct gcatactcaa ggagtagttc ctagcaatga agcagtagtg
    13021 gcgattgctt tgcaacaata cggtacacct actgctttga tcatgctagt agggatgatc
    13081 gtgaatattt tacttgctag attcacaaga tataaattca tttttcttac aggtcaagca
    13141 atgctttatg tttcttgtct aacagcagtc attttagtaa gcgcggggtt tactgcaaat
    13201 ttgtggatga tcttattagg cggggtcttc gaaggaacac tattaactat tacgcctgct
    13261 ctttgtcaac cgtttatgaa gaacatcact ggaaacaatc aagttgcgtt agggcatacc
    13321 ggaaatatcg gttatgcttc tagtggatgg atcggaaagt tttttggaaa caaagaccgt
    13381 tctacagagg acataaagat tcctaaaagt ttcagctttt tgcgagattc tactgtatcg
    13441 atcatgctct tgatgtcgat cgtatatgtc gcacttgctt tgttagctgg tcctggattt
    13501 gttgaaagag aactaagcaa tggagacaac gctgtaatct ttgcactgat ccaagctgga
    13561 acattcacag ctgggtttgt tatcgtcctt caaggggttc gaatggtgtt ggcagaaatc
    13621 gttccagcat tccaagggat tgctaaaaaa ctcgtacccg attctaaacc agcattagat
    13681 gttcctatcg tttttccata tgcacctaat gctgtattaa ttggcttttt tgttagcttc
    13741 tttgtaggaa cactatccat gtttgctatg ataggcttgc aaactacagt tatcattcct
    13801 ggggtagtag gacatttctt ctgcggagct gcttctggtg tcttcggcaa ctcgactggc
    13861 ggtagaagag gggcggtatt aggttctgct ttcaatagct tattgattag ttggctgcca
    13921 ttatttatcc ttccagtatt aggagatctt aaaatcgctt cttctacatt tgccgatact
    13981 gattatctag taccaggact tgtacttggg aaactaggag aatacggtca aatagccttg
    14041 acagttggaa tcatcgcttt ctttttactt gtcgtattat ctagctttct ttttgccgga
    14101 aaaagaacaa acattgtttc taaagaacag aattaagcaa acttcaaaat aaaaattgct
    14161 tggacaatat cagaagaaga tttaaagtct tctgatatgt cgcaagcagt tttttgtttt
    14221 atgcatttat ttttgaatag cttgttaaga caagtggctg ttttgctaga agaggataac
    14281 ttttaccatt cattttcact gttccgcctg ttctatcaga agtaatcacg atcttatctt
    14341 ttgttttttc aagcgaaatc tttccatctt tgataggtag cgtacaattc agtgtttcaa
    14401 atgaatctag attaggttgt atcataaatt cttcataacc tggctttgtt ggatacaaac
    14461 cgataaaata gcgtcctaaa agatagattg ggcttgctcc ccatgcatgg cataagcttt
    14521 ttccataagg atctccatac atactgtact tttcatttcc tgtttgagta gggtcgtatt
    14581 cttcccagaa agttaccgca tcatgagata acatccctcc ccaataatca agaatcgttt
    14641 ggtagacgat attcgtctgt ccgagtttgc ataaagcatc ttgttcatag aatttgaaat
    14701 atggagtagt aatctgtgtg atagtgtcat tcaataagac attctttaag atcgattgct
    14761 gttggtcttt gttagcaaaa tcgaataaaa cagcgaaaat attcgcatga cgcgtcacat
    14821 ttcttctccc agattcatag caatcgataa aagcgccaag ttcttcatcc caaaagtagt
    14881 caaataggtt ttgtttcaat agttgatatt tttcttgata gagatgagta tgataatttt
    14941 cgttctctcc atatgtttca gctaagatat ctccgcaagt gatcatggaa tgataggctt
    15001 tcagtaataa gacttgttct gcagcaagcg ttccttcttt atccatttca gaccaatcaa
    15061 taaatatcca atcttttgct ctcccataaa taaaccctaa ttcattcgtt tgctccatca
    15121 agtatctcat taatgattgc atcttagggt agacgatgcg taaaaattca tagtctttcg
    15181 tcatcatata gtggttttcg ataccaatca accacaagat cgaatagtca acgatagtat
    15241 tcatatgctg cttgatttca ttttgtcctc ttaatgctaa tatcgttcgt ttattgattt
    15301 cctcgtcaaa aaaaagatat tgattgatca tgaatgattg atacgcatct ccagaccaaa
    15361 tccagcgatc tcgtttgata ccgtcgataa aaaacaaatc actacataac ttgaaggctt
    15421 cttctgatac tgaccagatc tgattcagta aagaagaatc gcttgaaaaa gcagcttgtt
    15481 tcgaaagaga taaaaattca tgaaaaactt gaagttgaac cattccagta gaaacctctg
    15541 gtataaacaa atagcgaaaa gcgcgtttac gagctttcat ttttcttgtc attcctattt
    15601 gcttatagta gcaataatcg atatccatag cttctacatc ggactctcca taacatagag
    15661 tgatagattc gatatggtca tccagagata ttagttctac acagccattc actgctcgcc
    15721 caaaatcgaa taagattcct ccgccaatct ccgtttcagt gactggatgg acgcattctt
    15781 tttgataata gatttgattt ggatcttttg cgatatcgac ataaagcgga ctccagccag
    15841 ctggatattc ttcaataaaa ttacttgctg tccagccaat atctgatttt acttcgtctc
    15901 cctcgataaa catcgctggt agccctgatg tatgtcctac aaagatacga atttttgctt
    15961 taccagctgg acaagtcagc cattttccac atggatgttt ttgtccatta atttctacat
    16021 acccaactcc ttggatcgtc actttaaaca tggaaggttg atcaagaaag taatatctct
    16081 taaacttgac gttccgatgg caatcatcca tgtaccagta cgctggccag tcaaaacctc
    16141 gttcttcccg ttggaaattt tgtagcagtc catggcggat ctcaaagtct ccaggatacc
    16201 aaagccatct atctttatgt ttttcatttg tcattttaca cgacctccat caaatttatt
    16261 ttgttgtcaa ttgttctttt atttcttcat ctactcttat cactgtcttt ctattcgtcc
    16321 tgtgtactac tagaaggcta ataagcgcgc taatcaacag caagatactg ccattgacga
    16381 aaacagcaag tgttttactc ccaagagaag caaaagagag aaagagcaaa ccatatggtg
    16441 caatgaaagt acctaaatta gcgccaacca ataagattga agtattgaaa ctctccgcgt
    16501 acttgggcgc ttggatactt acttggttga ataagtaagg aatgaataac gaaaaagaaa
    16561 ttcctgttag gatcgcacca aacccaatca catacaggtt tatcacagtg gcaatcaata
    16621 caaagccgat tcccatcagt agtaaagaaa gtgtaagcat atagcgatgt gcttttgtct
    16681 gaagccagcc aaaacaaact ccggcagcca tacttgccaa ttgcatgatt gttaagatat
    16741 tcgctgcatt agtttcgttt cctagagaat aagtcgtcag gactgtggct aatttaatcg
    16801 atacagcatt atataccacg atgaccagca agagaaaaat accataacta atcattttag
    16861 gattcgtttt gattacttgt cttgttgggg ctgtgccatg cgattgaagt tcagttgttt
    16921 caggtgtagg aacaaacaaa acaaacaaga caagaatagg taagctgatg gcgtagacta
    16981 aaaaggtata ttgccaaccg aactgtacta gctgtcctgc aacaaatgtc attaaagctg
    17041 caccaaatcc ttgaaatgca ctttgaaaac caatcataga ggctcgttca tctccgtgat
    17101 aaaaggcagc aatcatgctg actgccaaag aattgatcaa tccaaagcca atacctagtc
    17161 cagctcttga agctaagaca atactgtaac tttgtgagaa gagggggagg ataccacaaa
    17221 tcgatgcaat cactaatccg atgatcacag tttgtttttg tccgattttc ctagcaataa
    17281 aactactaat caaaacaaaa atagtgacca taaaagaggg agcagtcgtt aataattcaa
    17341 cagcagataa agaacgcgtg gcataagtag ctgacatact gggaattgca gacgtgactg
    17401 caccagcaga agtcaaaata agagagactg acagcagaga aaatttcatc atctttttat
    17461 tcataagcat gttcctttca tttttgtaag cgcttatcaa aaatatagta tgaagtggtt
    17521 attctctcaa tagcggaatc acaggaagtt catgtcccaa ccatagaaag tctttatgaa
    17581 accaaaaata gctttgcata taatagcagt acagcgtgaa ctaataagcg aaagaaaccg
    17641 cttacaaaaa ggagggagtc aattggaact agctgaatta gacatttttt tgcgtaacga
    17701 agaatcaatc gaaactctgc agcgtgaaca aggaatgaat atcaatgatt taaacttagc
    17761 ttaccatctt cagacaccgc caaaaatcca ccgaggggat ctatttcaac agggaaatat
    17821 tcatatcagt aaacaccgtc gatatgcgga agttcccgag catacacatg attttgttga
    17881 aatcaattat atgtatagcg gtacatgttt acaaattatt aatggagaaa ggatagagat
    17941 ggaagaaagt gacgtggtga tcattgataa agaagctact caaagaatcg aatacacagg
    18001 gcaagaggat attttaatca acatcttatt aaaagaagaa accttatcta cagagatatt
    18061 gaatcatttg gcaagcacaa ataatctact ttcttctttt ttgataaatg catcaaaaga
    18121 aaaaggggaa catacacaat tcattcattt tcgcggcgga aaaacaagtc gtttgacgca
    18181 tttgattgaa aatatgctgt tgatttattt tgatacatac agtcataaga tccgttcgtt
    18241 aaatctctat acatcactat tactgatcga attgactcat ttattagaac aacaaagctt
    18301 ttcatcagca gatggatgcg cagaaaatga gatgcttaga gtattagatt atatcgataa
    18361 gcattttact tcttgtacgt taaaagaaac agcagctcat tttggctaca atccggtcta
    18421 tcttagtaat aaattgaaaa tgtattttga tgcttccttt caagaactgg ttctaaagaa
    18481 aaaattcgac tttgcgttgg aactgattca agaaacgagt ttttcgttgg aagaaatcgc
    18541 taatcgtatc ggatataaac aaacgacctc tttattcaaa ttattcaaga agtattcaac
    18601 atgtactccc aatgaatgga gaaataaaca ttaagaagaa aatctttcac taaagcatat
    18661 gaaggaaaat tgttttaggt gcgattgctg tcttattata tcgttgcagc gaattttttt
    18721 ggatttattt tcagttctaa acttttggtt gtattatcag gggatatagg tgtaaacatt
    18781 tgtcatttat ctgttatgcg tattcaggaa ttcgtttcca tcaaaaatta taataacaac
    18841 tattataatt aacatgttga taaaatgaat aaacaagact atacttttca tgaaagtaac
    18901 aaatagttat gaaaaggagc tgtttattca tgaaaaaaac aagtcgtaaa gtagtaatcg
    18961 tcggtacagg ttttgttgga acaagtatcg cttatgcaat gatcaaccaa ggaatctcta
    19021 atgaattagt attgatcgat gtgaatcaag aaaaagcaga aggggaagca ttggaccttt
    19081 tagatggtat ggcttggggc gatgaaaatg tggctgtatg gtcaggtggc tatgaagaat
    19141 gtaaagatgc tgatatcgtt gttatcacag ctggtatcaa ccaaaaacct ggtcaatctc
    19201 gtttagattt agttaaaaca aatgcgtcaa tcatgcgcca aatcgtaaaa gaaatcatgg
    19261 gatcaggatt cgacggtatt atcgttgttg cttcaaaccc agtagatatt ttgacatata
    19321 tcgcttggaa cgaatctggt ctgccaacat cacgtgttat cggtactgga acaacattgg
    19381 atacaactcg tttccgtaaa gaaatcgcat tgaaattaaa agttgaccca cgcagcgtcc
    19441 acggctatat cttaggtgaa catggtgatt cagaagttgc tgcatggtca catacaactg
    19501 tcggtggtaa accagtgttt gaaattgtag aaaaagacca tcgtattgcc caagatgaat
    19561 tagatgtgat tgctgataaa gtccgtaatg ctgcttatga gatcattgat cgtaaaaaag
    19621 caacttacta tggtattggt atgagtactg cacgtatcgt aaaagctatc ttgaataacg
    19681 aacaagctgt attgcctgta tctgcttatt taactggtga atacgacgaa aaagatatct
    19741 ttacaggtgt gccatcaatc gttgacgaaa atggtgttcg tgaagttgtt gaattatcaa
    19801 tcaacgaaga agaaaaagca atgttcagca aatcaacaag tgctttaaga gaagtattga
    19861 acactgtatt ataagaaaca atgaatatga actaaaaatc ctaaaactgc ttaacagctg
    19921 aaatagctgt taagcagttt tttattgata tctaataggt aagcaaataa acagaatggt
    19981 taaaattaat cggtccgaaa atgtatccgt acatatgaac atatgaaatt taatctgttg
    20041 atta
//
LOCUS       DAYYCY010000035        19367 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000035 DAYYCY010000000
VERSION     DAYYCY010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19367
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.035"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(407..766)
                     /locus_tag="ACHAZB_001028"
     CDS             complement(407..766)
                     /locus_tag="ACHAZB_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFM8613719.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(796..1137)
                     /locus_tag="ACHAZB_001029"
     CDS             complement(796..1137)
                     /locus_tag="ACHAZB_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8613720.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            complement(1134..1808)
                     /locus_tag="ACHAZB_001030"
     CDS             complement(1134..1808)
                     /locus_tag="ACHAZB_001030"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFM8613721.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     KFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(2984..4282)
                     /gene="asnS"
                     /locus_tag="ACHAZB_001031"
     CDS             complement(2984..4282)
                     /gene="asnS"
                     /locus_tag="ACHAZB_001031"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFM8613722.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(4322..5512)
                     /locus_tag="ACHAZB_001032"
     CDS             complement(4322..5512)
                     /locus_tag="ACHAZB_001032"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFM8613723.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(5533..6060)
                     /locus_tag="ACHAZB_001033"
     CDS             complement(5533..6060)
                     /locus_tag="ACHAZB_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HFM8613724.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(6107..8896)
                     /locus_tag="ACHAZB_001034"
     CDS             complement(6107..8896)
                     /locus_tag="ACHAZB_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HFM8613725.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNGKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(9043..9243)
                     /locus_tag="ACHAZB_001035"
     CDS             complement(9043..9243)
                     /locus_tag="ACHAZB_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8613726.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(9696..10016)
                     /locus_tag="ACHAZB_001036"
     CDS             complement(9696..10016)
                     /locus_tag="ACHAZB_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8613727.1"
                     /translation="MKKRLLWCLLSLSLSLTSQPVFAEETQRFPITGTRLYKNVYVQT
                     PDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(10519..11637)
                     /locus_tag="ACHAZB_001037"
     CDS             complement(10519..11637)
                     /locus_tag="ACHAZB_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8613728.1"
                     /translation="MAKKDKELLSQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            11870..11992
                     /locus_tag="ACHAZB_001038"
     CDS             11870..11992
                     /locus_tag="ACHAZB_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8613729.1"
                     /translation="MHLIMIFSLLSIISLFAFSASRLLDAETQPIPVKVRKNDR"
     gene            12108..13415
                     /locus_tag="ACHAZB_001039"
     CDS             12108..13415
                     /locus_tag="ACHAZB_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFM8613730.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSVT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETARLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAE
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            13536..14735
                     /locus_tag="ACHAZB_001040"
     CDS             13536..14735
                     /locus_tag="ACHAZB_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFM8613731.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYSSTWTFAVI
                     LTYFVVLEIFYSTFYFLIPLVFFSIFAFSYFSEKRRLLNGLLFNIFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVFLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCIPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYWAQSRATLNPPILLMSGGQGADEKVPE
                     AMAMKQYAMEQGIPERDILVETNSTTTLENMRYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(14762..16030)
                     /locus_tag="ACHAZB_001041"
     CDS             complement(14762..16030)
                     /locus_tag="ACHAZB_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HFM8613732.1"
                     /translation="MEKQLPSPVRILMYMLKWLVVTAILGVFMGSLSAFFLNSLTFVT
                     DIRLAHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNIAHLFRLDKAEREILVISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHAYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAVLA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAAYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRIKEKNKV"
     regulatory      complement(16086..16152)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            complement(16200..16841)
                     /locus_tag="ACHAZB_001042"
     CDS             complement(16200..16841)
                     /locus_tag="ACHAZB_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HFM8613733.1"
                     /translation="MEQTMKPYQYFFIKREVEQLLNAYSSVNDSKTVQTVQALAAEKI
                     RDVLGYEPSEIDTFLTTVLDVKLTKTQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQAHTYIGWNDPGTQKKFMVYYEDGKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKK"
     gene            complement(17250..17753)
                     /locus_tag="ACHAZB_001043"
     CDS             complement(17250..17753)
                     /locus_tag="ACHAZB_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFM8613734.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(17876..18640)
                     /locus_tag="ACHAZB_001044"
     CDS             complement(17876..18640)
                     /locus_tag="ACHAZB_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HFM8613735.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVIPLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            18748..19023
                     /locus_tag="ACHAZB_001045"
     CDS             18748..19023
                     /locus_tag="ACHAZB_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFM8613736.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
BASE COUNT         5548 a         4130 c         3446 g         6243 t
ORIGIN      
        1 ttcttccata cagatgtttt ggcttattct ttctttttct cttcgatcat tgcccctgct
       61 cttatccaac ccgcacttgc cttcttgatc ttgacaggat agcaactaag tttgaagcct
      121 gttgctggca atttgtctaa attcattaat ttttcgatag gacaatatct tttttcaatc
      181 cctgcaaaat gtccttccca gatattcgaa gctccactac gctttcaaaa atatagaaag
      241 agttctttaa tgaaaattat ttcaactttc cataaacagt aatcccatat aaataaaaga
      301 cgtaaaagtc acatgggaga acttatacag gaaaacacat agagtcaggt gaaatacatg
      361 tatcccacct gactctatgc gttttctaac ctttattatt ctctttttat acaatttctg
      421 caccgaatgg catcaacgcc aatcgagcaa gtttgaaaga ttgcttggca aaaggtatcc
      481 ctatgatcgt gatacataga aggatgccgc tgacgacatg tgcgagagca attggtaggc
      541 ctgaaacgat cagccagatg atattaacga tcaatgacat gccagactgg tgataaatta
      601 cttctttgcc aaaaggcgca aaagaaaggg aggcgatctt aaaacattgc ataccgattg
      661 ggatacctat gatcgtgata caccaaagta tccccgctgc taaccagcta agaccaccga
      721 ttagtccgcc gaatataaac cagatgacat ttcctaaaca actcattgta tttctccttc
      781 tattactgtt tttgattaaa actgggcaaa aattgatttt attcttcttt ttttagcttc
      841 cagcatcaag tattctgcaa attttttttg gatttcttcc gtattttctt ttgttactaa
      901 tgtgtgttgg attagttttc ctgatggtcg ctgatccacc cgttcttctg actgttgcac
      961 gtccgcttgc gcaaatccga taatccgttt ttcttttgtt acagcaacga gcaatagttt
     1021 attcttatca tttaggtcag ctgccgtttt tgcttttacg aatgattctg gatgatcagt
     1081 aatcagctga ttgatttgag gtaaatcttc ttttactgcc caccgtattt ccatcatttc
     1141 tcacttttct ttcgctcatt tttatctcca ctaaaaaaat cgactgcggc aattgcatct
     1201 atccagcttt gtgcaatgat ctcatgtccg ccaatcgttg ggtggacccc atcttctctt
     1261 gtatatttcg aaaaaccata agcaatccct aaagcattga atatgccatc taaaggaacc
     1321 agcaatgcgc catattcctt agcaagttta cgaacaattt ggattttagg gtctaagtct
     1381 ttacgccagc catgacggtc gtcggttata ggaagtacaa acggctccat caagatgatg
     1441 ttgcagattt ccgctgtttg aagttcattt agtaattttc gataaacgga ttcaaattgc
     1501 tgcgcagatt tctcagttcc gaatatagcc tgatcgctag tattatgcca ggtatcattg
     1561 attccgatca aaatcgatac atagtcaggt tgtaaagcta aacaatcttc ttgccagcgt
     1621 acttgcagat ctgcaatttt gtctcctcct atcccacggt tcatgcattc aaatttcatc
     1681 gttggatatt gggagattag ttttccggca atcaaaagtg gataaccttt acctagatca
     1741 gttgcttctg ctcggtctct tcctgcgtct gtaatgctgt caccgataaa taatatacgt
     1801 tttttcattt gtcaaaaact cctttttgaa taaaacgatg attttcttcc ttatattatc
     1861 ccataagccc tatcaagacg aaagagaaaa tatatgtata aaaaatgaca ccagcctagt
     1921 cccttcaatc aattgaatga acgctcatca agtgtctagt tttttatact ttttttctta
     1981 acgtgccgga ttccccttga ttccttcgtt gtttggtcca gagaaatcga tcacacctaa
     2041 gcttccgtca atcagaccac tgatgacgcc atcagaacat atatttatag gctcatctaa
     2101 gacttccagt aaatgttctt ttactaatat ctcttctaga ttgcttggga tagattggtc
     2161 ataactttat ctggtccgat gatttcaagg atgaattaaa cttacatatg gatcattgca
     2221 tgctgaaaat acggacctac tccatcttcg aatgttacta ttagtttgtt ttcaggtact
     2281 agatgctgct ttatttcaac agcagaagca tcatcaaatc agatttccat ttgaataacc
     2341 ttctttgtat atgtttgatt gattgcgatt tcgttataga accttgtgaa tcaaatagca
     2401 atttcttttg ctttcttctc ttgtctgctg gaaacttcct ttatcccgta ttttaaaaaa
     2461 agaactgcga gagttcgcag ttcttttttt taaataaacg ttgctctccg gccaactcct
     2521 cggtcacaag tcacaatagt catcaaacat gaaaaactgt tttttgaaat tactccttgt
     2581 gactcggagc tgaacggcag gcgcacaacc tcttgatata aacggtgttc aatcagctga
     2641 ccctcgatat tagctaaaaa aacggtaaaa tatgagaagc tatttttccg ttttgtttct
     2701 ctaatactca ggtcaaggca ctgatttcac aacctcttcc ttaaacggtg ttcaaaagcc
     2761 agtttctcgg tattaagcca aatttatgca aaaatgtgga aagccatttc cacaaaattt
     2821 ttcttaatac tcgaaactaa acagcttttt cacaacctct ccactaaacg gtattcaaaa
     2881 gcttacatct gcggtaataa gtcccgccaa ttcaaaaaca tgaacagctg tttttgcttg
     2941 gctgttctaa ttacttgatg cagaacagct ttttcatcac ctcttatgga taaattcggt
     3001 tcaacaaacg tgggaatgga atagcttcac gtacatggtc gataccagca atccaagtga
     3061 ctgttcgttc aaggcctaga ccaaaaccag agtgcggaac agaaccgtat ttacgtagat
     3121 caagatacca tgaatattct ttttcatcca aaccgtgatt acggatttgt tctagcaaga
     3181 aatcatagtc tgttgcccgt tcgcttccgc caataatttc accgtaacct tctggtgcga
     3241 tcatatctgc acaaattacc acgtcttctc ttgttggatg tggtttcatg tagaatggct
     3301 tgatggcttt tggatagttt aggataaaga caggctgttc gaatgaattc gcaatgaacg
     3361 tttcatgagg tgaaccaaag tcttcgcccc attcgatgtc atcaaatcca tttttcttca
     3421 gtaattcgat tgcatcatcg taagtaatac gagggaatgg caattttgta taagttttta
     3481 agacttcttt gtctcgtcct agtacatcta atgcatattc gcaatgatct aaaacatttt
     3541 gtactaagaa tgctacatat tgttcctgta cttctaaact ttcttcttga tgagtgaaag
     3601 ccatttctgg ttcgatcatc cagaattcga tcaaatgacg tcgtgttttt gatttttcag
     3661 cacggaaagt cggaccgaat gaaaagactt ttccgaaagc catagcagca gcttccatgt
     3721 ataattggcc actttgagat aaataagctt tttgatcaaa gtaatttgtt tcgaatagat
     3781 ccgtagtgcc ttctggtgca gaaccagtca agatcggcgg atcaattttg acaaaatgat
     3841 gttgattaaa gaattcatac gttgcacgaa tgatttcatt tctgatctgc ataatcgcat
     3901 gttgtcttga agaacgcagc cataaatgac gatgatccat caagaagtct gttccgtgtt
     3961 cttttggtgt gatcggataa tcgtggctct cgcttaccac ttccacattt gtaattccga
     4021 tttcataacc aaattttgaa cgactgtctt cacggatttc tccagttact aacacggatg
     4081 tttcttggtg tagattttta gctgtttgga aaacttcttc cgacacttcg ctttttacaa
     4141 ctacaccttg gaaaaaggct gttccgtctc ttaattgtaa aaatgcgatt tttccgcttg
     4201 atcgcttatt tgcgacccaa gcgccgattg ttactgtttc tcccacatgg tttttcgaat
     4261 cgatgatttg aatttgttcc acgtatgact ctccttttat attccttgaa tttcttcagg
     4321 attaattttg acttttactc tcaataaatt gcttgattct gcgtaccgct tcttctaacg
     4381 tagcgcgatc agtggcataa ctcatgcgga cattttctgg tgccccaaag ccttcgccag
     4441 tcacaagcgc tacccctgtt tcttctaaaa gatcttctac ccattgtgtg acattttcat
     4501 acttacacat gcgtagcgtt tcttttacat taggaaaaag atagaaagct ccttgcggct
     4561 tttcaagaga aacacccggc agttctgcca caagcggata taacgtattc aggcgttctt
     4621 caaaggcgtt ccgcatttct tctactgtat cttgctcacc tttcaatgct tcaacagctg
     4681 catattgact gactgctgtc ggattgctag ttgattggga agcaacagcg atcattccat
     4741 tgatgatcac aggatttccc accgcataac caatgcgcca gcctgtcatc gcatatgttt
     4801 tagacacgcc gttgatgatg atggtctgat ttttgattgc ttctgaaatc gttgcaattg
     4861 gagtaaactc attgccatta taaactaaac ggccataaat atcatcagca actatcaaaa
     4921 tatcattttt tactgcccat tcaccgattg cctgcagttc attcttgcta taaatcattc
     4981 cagtcggatt cgatggagaa ttgatgatta cggcttttgt tttttctgaa cgtgcttgtt
     5041 ctagctgttc aacagtaact ttccagctat tggattcttc ccctttcaca aagacaggaa
     5101 gtccttctgc cagttttact tgctcaccgt aactgaccca gtaaggcaca ggaatgatga
     5161 cttcatcttg cgggtcaagg atcgtttgga aaagggtata caaagcaaat ttcgctccgt
     5221 ctgttacgac tgtttgtgaa ggttcataga caagtccata gtttttcttc agataatcaa
     5281 cgattgcctg tcgaagctct gggattcctg ctgttggtgt ataatagcta gctttcccat
     5341 tgcggattgc ttcgattgct gcttcttgga tgttttctgg tgtcgcaaaa tccggttctc
     5401 ccacagtcaa actcagcaca tctctgcctt ttgcttttaa tgccttggcc tttgccgcag
     5461 ctgccaaagt gacagacggt tctaatcgtt ttgttctttt tgataactcc atcccattca
     5521 ttcctttacc ttttaaattc ccttgattgt cttgacttca tctccagatt caaagccaat
     5581 caggtaataa ttcagtttcc catttgtttc ttttcctact acttcccaca caggctgatc
     5641 atcatacatc cctaaagcag ctttttcgat ttggatttca ggatgggccg cagcaattgt
     5701 ctgttttgct tcgttttcag tcagtccctt tgtttgatcc aatactttta ctttttcacc
     5761 agatttagga atgataacgg cgatttcttt tcctttatca tttgtacctg tcagtgaaaa
     5821 gtatgtcttc tttcgagtaa accaataaaa atgatcggtt tcctcaagat ttgcatattt
     5881 ctttgcgatt tctactgctt ctttcttcgc ctgcaccgcc ggacgactgg aacgcaagta
     5941 aaaaatcgag ctgatcacga tgaccgctaa taaaaatacg atcaagccaa gtaacacgcg
     6001 attaatcgtt ttttcctttg tctgtttttt cttcaagggt cgtcgctcct ttatcttcat
     6061 tccttttcat tatagcagat tttaattatg aaaaaagaca gctcctctaa cttccttctt
     6121 catttttctt taagaattca tggatatcct gatagatttc ctccatagct agctctttga
     6181 tcggtacttt tttcggtaat gcatggatga tccgtgcgcc atattttgca gtgactagtc
     6241 ttcgatccaa aataagcaat acgccacgat ctgtctccgt ccttatcaag cgacctaatg
     6301 cttggcgtaa cttcaaagct gctttaggta cggattcctg gtaaaaagga ttgatcccct
     6361 ctgattctaa ataagcgttc ctagctttga ccatcgggcg aagcggatta tcgaagggta
     6421 aacgtgtgac tacaacgatc tgcaaagcat ctccaggcaa atctacacct tcccaaaagc
     6481 tgtcagcgcc aaacaatacg cttttttctg acaaaatgaa gcgtttcaaa agcttctccc
     6541 ggcttccacc aaacccttga gctagtattt ctcgtccttt ttctagcata gacagatgca
     6601 ttttctgata gaccttctgc aaaacatcat gagaagtgaa taacacaagt attggccgat
     6661 cttcttgttc gatcagacct gtaagtgctg tctctaaata atcgatatat gcttgattgc
     6721 tgacttgatg gacggcaggt gcctcttttg gcacatagat ccttgcctgt ttctcataat
     6781 cataaggaga ggcaatcact ttaagaggtg ctgcttcaat gccaagcctc ttagagagat
     6841 agtgtcgatt agggccgatc ttcagcgttc ctccaatata aagaatacga tcatatcgag
     6901 gataccaaac agtcgacggc aacatagccg cttgaaaatc atggagatga aaaatcatct
     6961 gctgcgctgt gttcccagga acgaaccaat ggatcaaatg atctcgccag tcttccagcc
     7021 attgatccat cagcaagtat tgcttgtcta attgatcaaa taggctatat aaacttgcta
     7081 aatcgatccg catctggttt gtccagcttt cttggataat ctcaaaagtc agacgcattt
     7141 gtgactgtag ctgctggatc tcctgataaa aaagctgcag gtgatcaatg gctgtcccag
     7201 attccagtgg cagctgatcc aacatctctt ttgtcatcat gacttcttct ggatactgtc
     7261 cgttaggttg cttgccgctc actaaactca ttaatccttc tgccatacaa atctgtgctt
     7321 cgcatagcgc agaaagttct tctcgataaa tcgataacca gcgtaaggct tcatgttctt
     7381 tcggtaaata gctttgtatt cggtcaaaca gctgatttgg ttcttctaat tgctggattt
     7441 gtttatggaa atagttaata cttaatcgtt tgccatttac tttttcagcg atttccggca
     7501 aatgatgtac ttcatcgatc aacaaaaatg gactagctgg aagcaatggt tcttttcgct
     7561 gtgtttcttg tactaaaaac gcatgattga caatcaaaaa attactttgc ctgattttct
     7621 tttgcaagaa acgaacaaag tcttgttcgt aaaatgactg atttcttgct aaaaatccaa
     7681 tacctctgtg cgcgatatct gcaaagaatg aatggttcaa atttgttaaa tgtaattcat
     7741 ccaaatcacc agtcttcgtt tgcgtcagcc aaactaagac cgccatttga tacaaagcat
     7801 attgtttttg ttctggcggt tccacaagtg tgcctttaaa acgctgcaaa tcaatatagt
     7861 ggcgatagct tttgataatt acaggttgta tgggctgatc tagcaattga ttcagcaatg
     7921 gaatgtcttt ttctatgatc tgctgctgca aaactagcga aacggtactg atgacagcgg
     7981 gtttttcagg tgtagccaaa taactcaatg gcagcagata accgattgtt ttacccattc
     8041 ctgtagctgc ttcgatcatc aaatcttttg attcatcttt tgtgaaatgg tcgtagaccg
     8101 cattcatcag tctgttctgt tcttttcgat acatcagtgt tttaccaaac attttttctt
     8161 ttgcttgttt ttttcttgga tatgtccgct cgccataatg gacggatgtg aatagttcca
     8221 cttctttttt ctgcaaagct agcccatcga cgatttctaa tgagggatca agtggctctg
     8281 gtttttcttt catttcttca atcacatgat aaatgaaacg actcgtatcc atacctgtgt
     8341 gtctacttaa tcgcgcaatc tgctctaacg tgatgattgg caattttctc atccgttctt
     8401 cgatcagcaa aagaagttga cctgtcactt cggcatcact atctgcctga tgaggattct
     8461 catgaataaa tccaaggctt tctgacaaat cagctaagcg aaagcttggt tccgtcggca
     8521 agaaaatttg tgcaagttct actgtatcga tgcctggtat cttcaaacta ggaagtccgc
     8581 atcgcatgag ttcatggttc aaaaaattat aatcaaaata aatattatgc gcgacaaata
     8641 tggtgtctgc caaaagattg taaatcgttt gggcgacatc ttcgaaataa ggcgcttttt
     8701 ggacgcgttg attcgtgatc cctgttaaat gctggatttg ttttgaaatc ttccgccctg
     8761 gatgaatatc gatcgaaaaa cgtgaagtga tccgacctga ttcaatcatg acgcagccaa
     8821 attggatgat ccgatcttct tttggattcg tcccagtcgt ttcgatatca acgactgcat
     8881 aagttcttgg tttcaaaata tcttcctctt ctcctgcctc taatgttctt ttgaaattat
     8941 actacaaagt gcagaaaaat actctcatcg ctttttaaag aaaatagaca aaaacgccca
     9001 gacacgcaaa tgtctgggcg tttatttttt agtataggtt aattaagctt tagtaacgtt
     9061 tgttgcttga ggtccgcgtt ggccttcttc aacatcgaaa gttactgctt ggccttcttc
     9121 taaagttttg aatccgtcgc cttggatagc tgagaaatga acgaatacat cgtttccgtt
     9181 ttctgcagtg atgaatccaa atcctttttc tgagttaaac cattttactg tacctgtttc
     9241 cataataaaa atcctccttg tgtatcaaca catattttgc aattacttga atggtgtata
     9301 tctggaagat gtttatttga aacaagctac cacagattac cgaacaaaaa ctactcctat
     9361 agtatatcat accgtttaca aaaaagctag agtttttcga ctttttttat taaaaaaagt
     9421 tttctcagcg attacataaa gtgaaaaacc gaactttttt ttaaaataaa cgacatattg
     9481 ttcagttggt gtttttttgc tctgatgttc ttttttaaac taaaaaaatt attctttctg
     9541 actacttatc ggttctgttt caacggtcga ggctggggaa gattctttag taggtctgat
     9601 gttctcatgg tatttctgat agcctatgac agttaccaga agaacaaggc aaaccaatac
     9661 gaccaacgag attagttttt cttttttgct catatctata cctcataaat aacaggatga
     9721 tgtccatcat atttctgagt atgagtaaaa gcaaaagctg cagctgtaga atgtaagaaa
     9781 tgccagcaac tttcataatc acaggtcgga cagtaccaaa agtaaccaaa tcttggctgc
     9841 aaagaccgtt cttctgtttc aggaatcgca gaatcaagat ctggtgtctg tacataaaca
     9901 tttttgtaca gacgtgtgcc tgtaatagga aaccgctgtg tttcttcagc aaaaactggc
     9961 tgacttgtta aacttaggga tagagacagc aaacaccata acagtctttt tttcataaag
    10021 acctccttat cgataagttt ttcccaatta aaatcatatc tcaagaacgc ccgttattca
    10081 cattttgttc ataaatgatg ttttttgtat cataatcgtc aaaaagaggt cgtgaccacg
    10141 tcacaacctc ttttttaaac ggtgttccag aagtagcttc ttcggaaata agccgaaatt
    10201 tcacaaaaat ttgagaaata atttttgtca atctcttctt atttcttgaa gctgaccact
    10261 tctgtcacaa cctcttcctt aaacggtgtt caaaagcttg catctgcggt aataagccca
    10321 ccaaacaaaa aatatgaaga gctatttttg cttgctgttc ttattacttg atgcagaaca
    10381 gctttttcac aacctctcat acacggtgtt taatcagctg accttcggta ttagctcaaa
    10441 aaacggcaaa atatgtgaaa ctatttttcc gtttgtttct ctaatactca ggtcaaaaca
    10501 ctgatttcac aacctctttt aataacgaag aatcgctgtc agactctttt catctggcgc
    10561 atctttttga tccaaaataa acacttttcc gccattaatg atcacatcat ctgctaatgc
    10621 attcaacact tgacgccgat catattctgt ttcaggatct tcaccgaaac catcagctaa
    10681 gtttgacgta gcgataaata aatgtgacac ttttccttct ttcgcagctg gaacgatatc
    10741 aactaactga tcgatgaatt tcttatctaa gagtttattg tattcttttg attcttgagc
    10801 tgcgagttca cttgacagct tcttcgcccc ttcttcgata tctctaaagg atagctgcgc
    10861 aggagaagca gaaatcgcaa cgtctgcaca ataataggga tttttagcaa tttttttgaa
    10921 catcgtctga ttttctggta gagcaaataa ataaatcggc cattgttctg gattttcgaa
    10981 agaatccttc aagtacgtat caactgcttg gtaatagttg acccaatcga tctctacttc
    11041 ttcatctttt ggatttacgc catggaaagt tactccatct cgggctccgc ctcctccatt
    11101 agaagagaag ttcaaaccgc cacctgtcag ttcagatccc aatgcagtaa cgacatcaac
    11161 cggtgcccct tctggtagag atacttcttt tacttgtttg ttgctgactt catacaattt
    11221 catggagtcg cggttcaatc ccaataaata ataagaatag ttgaactgcc catttttgat
    11281 aatagccagt aaataaggaa gatcacttac atagtattga tcgtctaccg ggatactcaa
    11341 acgatggata taggtttggt cagcagttat aatgaccgct acactcgtag ttgcactacg
    11401 ccaaaacgaa ggatctccta acaaattttc aattttttct tggaacgggg accagtcatg
    11461 ttcggaatat tttttctcaa aacgtttctt tgcttctttc gcaaaatttt tgaatttgat
    11521 ctgatctttt tccacatctt gatgggcgac atgcgtattc agcatgatcg tgacgaatgg
    11581 tcctttagcg ccttctgaag tcaattgtga caataattct ttatcctttt ttgccataaa
    11641 cgtacccctc ctaaaaaaat cattcctttg tttctatcta aagtctacca caacactatt
    11701 tttccataca ataaaacgta tctaaaaaaa taaaacggtt tctacggttt taaatttagc
    11761 tattttttta agttatcttc ataatttcct caattattta gccccagtct tcaactattc
    11821 ttcatgaaat gctgataaac taagaaaaag aataaaagag gtggatctta tgcatttgat
    11881 tatgattttc agtttattaa gtattatcag tttatttgct ttcagcgctt cccgcttatt
    11941 agatgcggaa acacagccta ttcctgtaaa agtacgtaaa aacgaccgct aaccttccat
    12001 aacgtgcgtt cttatcctaa agttttttca gaaacattga aactctctcc cctctctgat
    12061 aaaattttaa tttgtagcga aacaaattaa ttacaaggag atcggatatg tccataaaaa
    12121 aaacacgtct agtcctatta ttggctagtc tcattctttg ccttgcttct tttctaccag
    12181 ctgttgctgt gcaagctgaa gatactttca aagtaaatgc aaaagcagcc tttgctgtag
    12241 atgcgcaaag cggtaagatt ctttatgatc aagatggaga aaaaaccatg gggatcgctt
    12301 caatcacaaa aatcatcggt ctttacatcg tccttgatca agtaaaagaa gggaaactat
    12361 catgggatga taaagtttca atttcagatt atgcagaaaa tttgagtgtc accccagatt
    12421 tatcaaatgt tcctttgcat aaagaaaaca cctacaccgt taaagaatta ttcgactctg
    12481 caatcatcca gtcagccaac gcttcaatgg ttgccttggc agaaaaaatc tctggcagcg
    12541 aagcaaaatt cactgaacga atgaaagaac aattgaaaga ttggggtatc aaagatgcaa
    12601 cgatcgttaa tgcatcagga ctgaataact cttatcttgg cgaaaaccga ccagaaggta
    12661 ctggagaaaa cgacgaaaat caaatgtctg ctcaagatgt ggctatcgtt gcccgtcatt
    12721 tgatcttgga ttttccagaa atactggatg tatctagcac tacgacacaa atgtttggcg
    12781 aaaacacgca atcaccagta gaaatggtca actggaactg gatgctccca ggattcgtga
    12841 attataaaga aggcgtggac ggattaaaaa ctggtacgac tgaacttgca ggagcctgtt
    12901 tcgtcggtac gatcactaaa aacgatcaac gaatcatcac tgtcgtattg aatgcagacg
    12961 gccatgcgga aaacccaagt gcccgtttca atgaaacagc tcgcttaatg gattattgct
    13021 acgacaattg gtcagaaaaa gagcttggaa aagccaatgc aagtatccct agtctaaaaa
    13081 ccattgatgt aaaagacgga aaagaatcaa cagtgcccgt tgtgcttaaa agtcctgtaa
    13141 aagtatgggt acggaacgat atggatactg gaaaattaac aatcacacca actcttgaca
    13201 aaaaacaagt cactgacaat gagatccaag cacctatgga taaaggaaca aaaatcggta
    13261 ccgcaacgat caccttggca gaagataaat taggttatct tgaagacaac aacgcgccat
    13321 ccacagaaat catcacggat aaaaccgtag aaaaagcaaa tattttcgtt attggctggc
    13381 gacgcgtcgc agaatttttc ggtaacttgt tttaattgaa caaaaagaaa aaagcatctt
    13441 tccatgtgga agatgttttt ttgctttttc tagtttaaat aacagctttt ttcatctatt
    13501 ttcgttatac tgtataagaa aaaggagttg aatttatgaa tctaaccaga gatctgattt
    13561 ttttggttct ctatcttatt ggggtattct ccatcgttta ttggcgaaaa cagaaaaagg
    13621 atccttcctt gttcttttac agcagtacat ggacgtttgc ggtaatcttg acttattttg
    13681 tcgtgttgga aattttttat tcgacctttt attttttgat tcccttagtg tttttcagta
    13741 tctttgcctt ttcctatttt tcagaaaagc gaaggctgtt gaatggtctt ttattcaata
    13801 ttttcctcat tagttttggt atctacttgt ttgtcttgct atacgaaaca caaaatattt
    13861 ttcttggcgg actgattgcc ttgatcacga tcccgttact gcttgtcttt ctattcggca
    13921 tttatggatt gatcgttttt cttttttgga acggtgtcac agttttacgt agagagtcgc
    13981 attcattagc taatctttta acgttgatac tagccatttt tcttacattg tttcttgttt
    14041 ttgacttttt cttgttaaaa tatttaccac aatggataaa tgctttgttt ttctgcatcc
    14101 cattgatctt gatctatcta tttatcgtat tctacaactt tttgactgtc tcattcttgt
    14161 atcagttcaa ccgccctcga tacaaccaag acttcatcgt tgtgttaggc gccgggttga
    14221 tcaatggaga aacagtttct cctcttcttg ccaagcgaat caataaggcg attgcttttt
    14281 attgggcaca aagccgtgcg acattaaatc cgcctatcct attaatgtcg ggtggacagg
    14341 gagcagacga aaaagtacca gaagcaatgg cgatgaaaca atatgccatg gaacaaggaa
    14401 ttcctgaaag agatattctt gtcgaaacca attcgacaac cactttggag aatatgcgct
    14461 attcaaagga aatcatggat cagcaaatga aaggtcccta tcgcgcgatc ttcagttcca
    14521 acaattacca cattttcaga gctggtctct atgcacgaca agcgaaactg aaggcaaatg
    14581 gcattggggc aaaaacagct ttctattact tgcctaacgc gtttttaaga gaatacatcg
    14641 ccatcttgat gatgaacaaa aaaagacata tgatcatttg cggattgctt ttcgcaggtt
    14701 ccgcattctt atcactgatc actttacttt tctaaaaaaa caaggtgtga caaaacatat
    14761 gtcacacctt gtttttttct tttattctct tgacgattgg caagaatagt attccttttt
    14821 tgacggctac ttgctgtgca gaataaatac cgctgtttcc tgaacacata aagctgatca
    14881 agcagatcat aaagaagtaa gcagcagctt gtgaaccaaa taattcgatt cccatcacaa
    14941 aacaggcaat cggtgtattt gtcgcaccag aaaatacccc gataaaacct aatccagcaa
    15001 gaaaaggaac agaaacatgg agcaaaggcg ctagtactgc gccaagcgtt gcaccaatct
    15061 caaatagtgg tgtcacttct cccccttggt accccgcacc taaagacaag accgtgaaga
    15121 ataatttccc aataaaatca taagcatgtg cctgtccatt aaatgattcc tgcagtaacg
    15181 gaagactcaa cccaaggtaa cgttgtgttt gcaaaatcaa cgctgcggct acaactactg
    15241 ctccacctat cgtatttcgc caaacaggat gaggaatcca ttgcgcatac cgcgctttta
    15301 aaaagacgat cgaacggctg aatacccaac caatcaaacc aaaagcaatc ccagcaacaa
    15361 aaactttgaa gaacagcaac atactccatt caggaatcac acccatttgg taatgtagat
    15421 gagaaacacc atagctttct gtcatgaaat ttgcaaaaag cgcagcaaag aagctaggaa
    15481 atattgcttc tgttcggact ttaccgattg ccaacacttc taatccaaat aatgttccag
    15541 ctaaaggtgt gccaaataca gaactaaaac ccgcactgat tccactgata accaaaatct
    15601 cccgttctgc tttatccaat cggaataaat gcgctatatt atcagctaat gctcccccca
    15661 tttgaactgc tgttccttca cgaccaacag atcccccaaa taaatgggta gtgatggtcc
    15721 caaacaaggt caatggaatg agccgcaaag ggatcttctc ttctcctcca ttcccttgat
    15781 cgatcactaa gttattccca cgactagaaa gaccgccatg atacgcataa agataagcaa
    15841 aaagcgcgcc gctgactggc aatagataaa gtagccaagg atgtgctagt cggatatctg
    15901 tcacgaacgt caggctattt aagaaaaaag cagacaagct ccccatgaat actccgagaa
    15961 tcgctgtgac caccagccac ttcaacatgt acatcaaaat acgcactgga ctcggtaatt
    16021 gtttttccat aaaaaattcc tcctcaaata aaaaaacgcc tactaaagaa aggggctggc
    16081 cccagacttt agtaggcgtc attagctatc ttacgttagc atttaaaggc gaacaccatc
    16141 acctattcac ttccattaaa agatagcata tttttctgta aaaaacaata aaattatcgt
    16201 tatttcttta cacgttcagc aaattcatag aaaggagcca atttcgttaa ctgttgatta
    16261 cattgatcac tatcatgaca aatataattc ccctttttcg tatacgtgcc atcagaaccg
    16321 gctttcgttg tagaaagaaa gagcgagaca ttcgatgttt tctgacagat cgcacagatt
    16381 cccttattaa tcgttggtga taaagtaccg ctgatccctt ttaattttcc atcttcgtaa
    16441 taaaccatga attttttttg cgtccctgga tcattccaac cgatgtacgt atgtgcttgc
    16501 aagtcgattt cattccaagc gggttgtttg agttttttca cttttcggaa taatttctcg
    16561 atttgtggtt tgctcggctg cataaacggc tggacgattt gcttcagctc ctccaatgct
    16621 cgttctgctt gcgtttttgt cagttttaca tctaaaactg tcgttaaaaa cgtatcgatt
    16681 tctgatggtt cgtaacctaa tacatcgcga atcttttcag ctgctagagc ctgaaccgtt
    16741 tgtaccgttt ttgagtcatt gacagatgag tacgcattta ataactgctc tacttctctt
    16801 tttataaaaa aatattgata tggtttcatt gtttgttcca tgattttaaa gtcctttctt
    16861 ctaaccaaag tgcatacttt gtagaaaaaa gtgatcttct gcaaagtatg tgttacctga
    16921 agatcccgga ctcttttaaa taagatacgt tttgcatctt atcatcaccc cctttaatgt
    16981 tcattcttct atgtacatga ttcattcgat aaataaaagt aaaccgtatc ttgagcagat
    17041 agtcaactgc aaacagaaaa aggactgtga caaaagtcgt gtcaaagtcc ctttctacaa
    17101 acaaacggtg ttccagaagt agcttcttca aaaaataagc cggaatttca caaaaatttg
    17161 agaagcaatt ttcgtaaatt ccttcttatt tcttgaagct gacccacttc tgtcacaacc
    17221 tcaatatggt ctattattgg ttttacctgt taaatcgagg attcttttcc gaagtaacgt
    17281 tgccatctct tacgcatact tgctgacata ggctgagcgg cttctttgac tgcgcagtat
    17341 ttgtctacga atgttacgat atatccttct ttgtacttag gcggtgcaat cgttgcgccc
    17401 cacatgtgct tcaaaatgat atctcgctca agatccgaca aatcggtgat cttttctgca
    17461 ttctttacag cgattcttgg atggatatac gcatgtgtgc cttcatcaaa tttcgtcgta
    17521 cgccaatcat agtagaacag atcatgcaaa agtccagcac gcgctgttgc tttagcattg
    17581 ccaccccatt tttttgcgag cagataacta tagtaagaaa cactgatgga atgctccaaa
    17641 cgagtggaat gaacatgctg tgtgtaattc gctaattttt tcactgcttc tgtttctaaa
    17701 agatcttcta cataagacat atattcttcg tcttcacgcc atttttctgt cataattatc
    17761 gatctgccac ctttttgaat ttcattttct tctgaaattg atctaagtat accaccagtg
    17821 ttcggattat tctagtcgcc ttggataaaa tagccaaagc ttaatagttc cttaatcata
    17881 aagaaaaatg ataaagaaca atccctgccg caaccccaac attcaacgat tccgcatttc
    17941 ctttcatcgg gatatatacg ttctgatcag tcaaagacaa gatttcctga cttaccccct
    18001 gaccttcgtt ccccatgatt attgcgaagt tatctgattt ttctactttg tctaaggcgt
    18061 gtgcttcttc atttaattct gtgccataca ctgtaaaccc tttgtctttc attaaaggaa
    18121 tcacttctgt caaatctttt tgcaacacag gcaaatgata attgcttccc tgcatgctgc
    18181 gcagcacctt tgtactataa atatctgccg ttcctttgcc taataaaaca ccagccatat
    18241 caaatgcatc tgccgtacgg atcatcgtcc ctacatttcc aggatcttgt acattatcta
    18301 ataacagcca tcctcctgaa aaatctggcg tgccttcagc cgttaacgcc atcactgcta
    18361 agattccttg tggtgtaggt agatccgata cagcatccat gacctcatcc gaaactagaa
    18421 aagtagaaat ctctttgttt gcggccagcc agtctcccca ttctttcatg cctcgctgtg
    18481 tcacaaaaag ttcttttatt tctgcatcag acgacgcagc ttcttcaatc agatggaacc
    18541 cttccaaaag atattcttcc ttttcttccc gatatttctt tttatgtagt ttttttaatt
    18601 ctttgattct tggatttttt gctgattgga tctctttcac attcctcacc tccccatgat
    18661 tatatcatgg aataggtgct tgagcttgtt tcatcctaaa aaaatcggta tcattaattt
    18721 ataaaatagg aaagaaaggt gacgaacatg cgcaaagtta aaatgaacgt ccaaggacgc
    18781 gtccaaggcg taggatttcg ctacatgacc aagatggtcg ctgatcaact aggcgtgact
    18841 ggaacggtaa aaaacgaaga tgacggctca gtcacgatcg aagcaatcgg caatgacgac
    18901 atcatccaga aatttattga agaagtcaag aaatctccga gcccaagtgg acgagtgcag
    18961 tatgtggatg tccaagaaga cccgatgatt gaggaacgaa agaaatttga tgtggtggga
    19021 taaggttgtg aaaaagctgg tctgcatcaa gcattaagga caggttgtga aaaatagctc
    19081 tccatatttt tcaacaggct ggacttaatg ccgtagatgc aagcttttga acaccgtcaa
    19141 taaggttgtg agaaagtcgc ttagttttga gtagtaagga gaattttgtg aaaatggctt
    19201 tccacatttt tgcataaaat ttgacttaat accgagaaac tgacttttga acaccgtcga
    19261 taaggttgtt tttgagacgc tttgcctcaa gtattaaaga taggtagtaa aaaagctgat
    19321 ctataacttg caataaattt gcttaaccca tattttccag acagaac
//
LOCUS       DAYYCY010000036        19363 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000036 DAYYCY010000000
VERSION     DAYYCY010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19363
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.036"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            265..2736
                     /locus_tag="ACHAZB_001046"
     CDS             265..2736
                     /locus_tag="ACHAZB_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFM8613737.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(2839..4575)
                     /locus_tag="ACHAZB_001047"
     CDS             complement(2839..4575)
                     /locus_tag="ACHAZB_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8613738.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(4572..5276)
                     /locus_tag="ACHAZB_001048"
     CDS             complement(4572..5276)
                     /locus_tag="ACHAZB_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8613739.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            5407..5910
                     /locus_tag="ACHAZB_001049"
     CDS             5407..5910
                     /locus_tag="ACHAZB_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HFM8613740.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            5870..6208
                     /locus_tag="ACHAZB_001050"
     CDS             5870..6208
                     /locus_tag="ACHAZB_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HFM8613741.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6229..7647
                     /gene="ffh"
                     /locus_tag="ACHAZB_001051"
     CDS             6229..7647
                     /gene="ffh"
                     /locus_tag="ACHAZB_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFM8613742.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            7759..8337
                     /locus_tag="ACHAZB_001052"
     CDS             7759..8337
                     /locus_tag="ACHAZB_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFM8613743.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8341..8862
                     /locus_tag="ACHAZB_001053"
     CDS             8341..8862
                     /locus_tag="ACHAZB_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HFM8613744.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(8941..9495)
                     /locus_tag="ACHAZB_001054"
     CDS             complement(8941..9495)
                     /locus_tag="ACHAZB_001054"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFM8613745.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            9748..10023
                     /gene="rpsP"
                     /locus_tag="ACHAZB_001055"
     CDS             9748..10023
                     /gene="rpsP"
                     /locus_tag="ACHAZB_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFM8613746.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            10035..10286
                     /locus_tag="ACHAZB_001056"
     CDS             10035..10286
                     /locus_tag="ACHAZB_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HFM8613747.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            10411..11202
                     /locus_tag="ACHAZB_001057"
     CDS             10411..11202
                     /locus_tag="ACHAZB_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8613748.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            11372..11893
                     /gene="rimM"
                     /locus_tag="ACHAZB_001058"
     CDS             11372..11893
                     /gene="rimM"
                     /locus_tag="ACHAZB_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFM8613749.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            11883..12644
                     /gene="trmD"
                     /locus_tag="ACHAZB_001059"
     CDS             11883..12644
                     /gene="trmD"
                     /locus_tag="ACHAZB_001059"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFM8613750.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            12780..13127
                     /gene="rplS"
                     /locus_tag="ACHAZB_001060"
     CDS             12780..13127
                     /gene="rplS"
                     /locus_tag="ACHAZB_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFM8613751.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            complement(13259..14395)
                     /locus_tag="ACHAZB_001061"
     CDS             complement(13259..14395)
                     /locus_tag="ACHAZB_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8613752.1"
                     /translation="MWIETKTDKNGKKVYKYNERYIDPKTRKRKKVSITYKNKSRETQ
                     KVALLELNKKIDIKLNEKTLQKPDLTFHELVEEWLVIYKRQVKESTYYPTNNILNTIK
                     KKIPETYIVSGINTVDLNNIFEDMIYKDDLSNKYVSVIKSKLNLLFSYAMKKGYVEKN
                     PINEVVIDYKRESKTIKIKDKFLEDDEYKRLVNFTTSHNKRYSLLFQWLYLTGMRPGE
                     AIALSKDDVHITNNNASVVINGTMMYRERSIADMKKSDSTKTAAGMREIDLPKKAIAI
                     YNELLELNPNGQFLFQTTKGTPFQLTAINTYLRNHKADMKIDKKLSSHIFRHTHISKL
                     AELGTPLYAIQDRVGHENSDITEKIYLHVTKGVKEKLKEDIEKL"
     gene            complement(14511..15425)
                     /locus_tag="ACHAZB_001062"
     CDS             complement(14511..15425)
                     /locus_tag="ACHAZB_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HFM8613753.1"
                     /translation="MKKIVFGLLFLGLVLSGCSTSPNSASTKENTVSSTSEYETKKLT
                     SSDVHKIKVGDDLTQVYQTLGSPLRKWTNDFVYKELDNAITRDELIVSISKDAQGDYY
                     KEHSELIEHGNSAKDIKKLEMLQYTYSTDSDDETMLIWVSPKTEQVVYYNQRTFLDKD
                     GKSKDSSSTTTTTTTSSSEPQTASVAHSIGEAVPFTSENGNSINVTVQSVSKDYGDDF
                     YKPEGLFYAKVDFLVENTGTTPFDASSSYLEFYDSSDIKSDLASRDYFSETIQPGKSA
                     QGTAYFEVKNDGTTFEVFFADTSWKGSY"
     gene            complement(15516..15944)
                     /locus_tag="ACHAZB_001063"
     CDS             complement(15516..15944)
                     /locus_tag="ACHAZB_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFM8613754.1"
                     /translation="MEMDTINLVEELKRKYQSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRGRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKRIEELKNLYGLPESAYRFLI"
     gene            complement(15949..16323)
                     /locus_tag="ACHAZB_001064"
     CDS             complement(15949..16323)
                     /locus_tag="ACHAZB_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8613755.1"
                     /translation="MNLLDRIKELAHKRGISITQLEEELNIPKNTIYQWKNRTPSTKR
                     LQLVADYFNVTTDYLLGRNQVPDWATKDEVVELDKLLDSNVNMSYGGETLTPEQVQRV
                     KDILIGTFWDIVKEDKEKGKKM"
     gene            16609..16794
                     /locus_tag="ACHAZB_001065"
     CDS             16609..16794
                     /locus_tag="ACHAZB_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8613756.1"
                     /translation="MSENLDLKIRAEMRKRRMTFKELAALIGISGAYLSDILNGNRDG
                     KKAQQHIETVKKILDIR"
     gene            16806..17054
                     /locus_tag="ACHAZB_001066"
     CDS             16806..17054
                     /locus_tag="ACHAZB_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8613757.1"
                     /translation="MTKLKKQDFVKKYNYSPSTYQRRMSELKNTAIFSAAYERVTGQE
                     VWINTELYDKFLSFKSYNRLRTRKVTPKEFIEKHLVDL"
     gene            17085..17264
                     /locus_tag="ACHAZB_001067"
     CDS             17085..17264
                     /locus_tag="ACHAZB_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8613758.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(17307..17777)
                     /locus_tag="ACHAZB_001068"
     CDS             complement(17307..17777)
                     /locus_tag="ACHAZB_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8613759.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            17864..18562
                     /locus_tag="ACHAZB_001069"
     CDS             17864..18562
                     /locus_tag="ACHAZB_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HFM8613760.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            18740..19081
                     /locus_tag="ACHAZB_001070"
     CDS             18740..19081
                     /locus_tag="ACHAZB_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8613761.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            19074..>19363
                     /locus_tag="ACHAZB_001071"
     CDS             19074..>19363
                     /locus_tag="ACHAZB_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFM8613762.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTET"
BASE COUNT         6374 a         3382 c         3813 g         5794 t
ORIGIN      
        1 tcctttggta ggatgacgtt ttggtcgact aaattttacc aaaagtgagt ctctttttga
       61 gttgaaacga agaaaaagtg ttagtagaaa atcgccttcg attttccacc aacacaaaat
      121 attatagaac cctttttttt tgctatatga caggaatgaa aggtgtttgc aagcttaatg
      181 tgaggaacac tttccatatc tgttctatac tggataggag aagtttggaa atatccagaa
      241 aacgaaatca atcgaggtag ccacatgaag aataaatgga taaatcgaag taaaaaaaag
      301 aaagtaacac aaagatcatc agataaagtc atccgtcttt ttccatcgta taaagaagga
      361 ttaacggcag atcaagtcaa ggaacgaata gaaaaaggtg cagcaaataa ttcagtcgat
      421 cccacattca aaacaaatca gcaaatcgta ttggaaaaca tatttacgta tttcaatttg
      481 atctttctta tcttagccat tttgctttgt ttagtagaat cctataagaa cttgactttt
      541 ttaccagtta tcatagccaa tacaggaatc gccatttttc aggaaattcg atccaaaaag
      601 atcttggacg aactaaatgt tttaaataca gcaaaagtca atgtcatacg agacgggata
      661 gaacaaacaa tagatataga agaccttgta atagatgaca ttgttatttt agagactgga
      721 tatcaaattc cagcagatgc agaggtcgtg gaagggaaac tgcaagttaa tgaggcttta
      781 ttgacaggcg aagcagatga aataaccaaa gaaataggtg atccattaat gtccggcagt
      841 ttcattgttt ccggaaaagc ttacgcccga ttagacaaag ttggagcaga ttcgtatatt
      901 tcccagctga cagtcaaagc caagacaatg ggagaaggag aacagtcgga gatgattgct
      961 tctcttaatc aattgatcaa atggattggg atcatcatta ttcctatcgg tatcctgctt
     1021 ttttctcaaa gttacttttt taatggaaac acgatcaaag aaagtgtcgt tgcaatggaa
     1081 gcagcattga tcggtatgat acctgaagga ctgtacttgc tgacgacgat tgcattagca
     1141 ttaagtgcga ctaaactagc aaagcaaaga gttcttttac acaatatgaa aagcattgag
     1201 acattggcaa gggtaaatgt cctttgtgta gataaaacag gaacgatcac agaaaataaa
     1261 atgtctgttc aaaaaatgat cgtctctaaa aaacaagaaa taatcagtca gtcagatcaa
     1321 ttggaagcat gcattgctga ctatgcaaaa gcgatggcag gagacaatgc aacaatggaa
     1381 gctgttaaag catattttac aaaaaccact gatcaatcct atcaccatgt tattcctttt
     1441 tcttctgtcc aaaaattcag cagtgtagcg ttggcagaca aggtgtatgt tttaggagcg
     1501 cccgaaatgg ttttgcgcga tcaattgccg gaatatgcgg aagagtttgt ttctttcgct
     1561 gaaaaaggct atcgcgtgtt ggtctttggt gtgtacgatg ggattcttga agaaccagta
     1621 ttgacagaag cagtcacacc attaggctat attctgattg ctaatcctat cagaaaagaa
     1681 gcaagagcca catttgatta cttcaaaaaa cagcaagtag ccatcaaagt gatttctgga
     1741 gataatccgc tcaccgtatc caatgtagct gtccaagcag ggatttcaca tgctgatcag
     1801 tacgttgatg catctaaatt atcagaagaa gaatatgaag cagctgtcga aaaatataca
     1861 gtttttggac gcgtcaagcc agaacaaaag aaaatcttcg ttcaattgat aaagcgtaac
     1921 aatacagtag ctatgacagg tgatggcgtg aatgatattc tagccatgaa agaagcagac
     1981 tgcagtatag cgatggcttc cggaaatgaa gccgccatgc aggcctcaca agtggtctta
     2041 cttgattctg acttttcacg catgccagaa gttgttgcag aaggacggcg cgttgtgaac
     2101 aatattgagc gatcagccag cttgttttta gtgaagaata ttttctcctt tttgctctca
     2161 ctgttttcag tgatctttgc tctgacctat cctttagaac catcacaaat cacattgatt
     2221 agtttgttta ctatcggact tccatctttc ttgcttgctc tagaagaaaa caaaaagcga
     2281 atcagaggga aattcatcat gaatgtgatg gaaaaagctg tgccaggtgg actaacagat
     2341 atgatcgtag tcggtgcgtt ggtgatctgc ggggtgactt tgaatctaaa taaaacagat
     2401 gtttctacag catctaccat gcttttgatt gcagtagggt tcctcgttct ctataaaatc
     2461 tgttcaccgc tgaataaatt cagaagtcag atcattcttt tctgtgctag cggtaacttt
     2521 ttttcggtca tctttttgca taagctgttt tcaatcaccg gtatctcagc tgtatcactt
     2581 ttgctgttga gcattctatt tttctcggca gattcgattt ttaggcattt gaccacattg
     2641 gtagaatatt tgtttgaacg taaaaatgaa aaagagcaaa agccgtttag tcgcgtagct
     2701 aaaaaatttc tatcgatatt taaacgagaa aattgacgaa aagtatctca aaaaagagta
     2761 aaaaagaaca accactcgtg tatctttcga agcaggatga gtcatccaac tttaaaacgc
     2821 acgagcagtt gctctttttt acattttcgg aatggtaacg gtgaaagtcg aacctgttcc
     2881 aagatgactg tctacagtaa tcgtaccatt aaggagttgc acgtagtctt ttacaatcgc
     2941 taaccctaat ccggtcccgc ctgaatgtcg gcttcgcgcc ttgtctacgc ggtaaaaacg
     3001 ctcaaagatc cgttcctgat cctcaatgtc gatacctata ccaaagtctt ggacagaaaa
     3061 cgatagatca tctgttgcct gatagcggat aataatctcg ctgtcgcttt ttgaatattg
     3121 gacagcattt tcaattagat ttttgataat tggataaagg atatctgttt tagtagtaaa
     3181 tggttcgttc tttccaatca atcgaatcgt caaccgtttc ttttcgatga ttggctgata
     3241 attttgaacg atttgctgga aataaggttc tgtataaatt gtctggatct cgtaagaatt
     3301 ctcgccatct tttgacagct ggatgatttc ccgaatcagt ttatctaacc ggatcgcatc
     3361 tttttgcatg atctcaagaa aagaagtcag agtttgcggg tcatcttttg caccatccag
     3421 taaagtctcg gtaaaaccaa tcagtgaagt cacaggagtt tttaattcgt gagagacgtt
     3481 gccaacgaaa tccttttgta gcttttctaa acgtcgcact ttcgttaagt catacgccac
     3541 ccctagaatc tgtccggtac cttctgaatt agtaaagtag cgcaaactca agtctaatgt
     3601 ctgccgacct tctgtaaggg tgatctcctg atggatcagc ggatgctcag gagtcacttg
     3661 atgaatcagc tgaatgagtt ttggctcttg aatgacttct gtgtaccgct gttcaggttg
     3721 atagcttacc acgtttaaat gtagctgcat ctttggatta agcagtagta aacgagaatc
     3781 agcatcatca atcaggaata cgccaatcat cagttcgttc agtaacgtat ataattgctc
     3841 ttctgtggta gtacacgccc gatacatctt actcatttgt tcgctcaacg cattaatcgt
     3901 ctgatacaat tcttcccatt gatcgggtgt ttgcatgatt acttccgcct gattaggatt
     3961 ttgcaccatt ttttttagta cgggcagcac ggttttcaat ggctggtttt tttgatagat
     4021 cagataatag atcattcctg caagaacaag gaagaaagac agaaagaaga aaaagaccca
     4081 acggcgaaaa ctctcagttc tcggtaaaaa accgcttgtc ggttctgcga tacgtaaaat
     4141 ggcttctaac tggccacttt ttttgactgg taatgcgaca tataaaagat cctcattcaa
     4201 agtagtactt ttgcgcaaag cgctacctag gttcccacca ttcaatacgg ctttgatttc
     4261 gggacgggag tttcgctgtt cattcaatgt ttcattgttt gtgtcaaata agatctttcc
     4321 agtagcatcc attaaagtaa tccgttcgtt cgactgatgg acgaattctt ccaaagcagt
     4381 tttgttttgc gctgcttgga ggttttcgac atctaattgc cggatcagca gttgtgcttt
     4441 ttttgtcagg tagctttctt gctgttccac gatctgctgt tgaaaataat gggaaatcaa
     4501 ttgccagcca ccaaaaaaca aactaactaa aagaataaga aagaagagac gttcataaat
     4561 ttttttccat ttcatcgttt gggttcctga aacttatagc caaatcctct aatagtcacc
     4621 aagtactttg ggtgttttgg atctcgctcg attttttctc gcaaatgact aacgtgtaca
     4681 tctacgattc ggctttgtcc tgcgaagtca aaattccaaa tgcggtctag caatgtgtct
     4741 cggtcgatca cacggtcttt tcgtttcatg aaataaacaa gtaattcaaa ttctttcggt
     4801 gttagttcaa tcagatggtc gtctacggtc acttgataat tggtcaaatc agcagtgatt
     4861 tgtccaatcg atagatattc aggctcagct tcatcttttg attgcttacg aggttcaatt
     4921 cgtctgaata ttgctttcat acgggctaat acttcgcgag gactaaatgg tttggtcaaa
     4981 tagtcgtcag cacctatttc taggccaatg atccgatcca cttggtcatc tttagccgtc
     5041 agcatcaaaa tcggtgtttc atttttttct tgacgcagcc gctgagtgat ctccatgcca
     5101 tccatcgttg gaagcatcac atctaaaatg atgaaatcaa atgattgttc taatgctaat
     5161 tctaatcctt cgcctccatc tgtggcactg gttaccttgt agccttcttt ttccaaattg
     5221 aacgtcaaga gcgttacaat agaaggttca tcatcaacaa ctagtatttt tttcattcaa
     5281 atctccttta aataggtttc attctgttta tattttagca tagaatcagt acaaagggct
     5341 attttacaaa agaaataaat ggtataatag gaaggcactt tgctgaatag aaaggaagag
     5401 cagtcaatga ttggaatcag tgcatgttta ggcggtctgg cctgtcgcta tgatggacag
     5461 aaaaaagaag taccagaatt gatggaacta gttgaaactg gagaagctgt catggtctgt
     5521 ccagaagtga tcggcggatt accaattcct agagatcccg ctgaaatcat tggtggaaat
     5581 ggattcgatg tgtggaaaga tcaagcaaaa gtatggacga caagcggcga agatgtaacg
     5641 gaagcgtaca aaaatggcgc gatcaaagcc tatcaaaaat tgcaggaaaa acacattgat
     5701 ctagtcatca tgaaggaaaa aagcccttca tgcggaaccc attcgattta tgacggaagt
     5761 ttttccggaa taaaaaaaaa tggtgccgga gtggctactg cttattttat tcagcaaaaa
     5821 atgactgtgc tctctgatga agaatggctg aaaactagag gtgagcagaa tggaaatcga
     5881 aaagaccaat cgaatgaacg cgctctttga attttattcg accttattga cagaaaaaca
     5941 aatgaactac atggaattat attatgcaga tgatttttca ttaggtgaga tcgcggaaga
     6001 atacgaagta agccgacaag cagtatatga caatatcaag cggacaagca agatcttgga
     6061 ggattatgaa aaaaaactcc atcttttttc taattatatt gttcgagaac aggtattgga
     6121 aaatatgact gcatatatta cagataagta cccggaagat aaaaaactac ttgaatatgt
     6181 acagcaaatt caagcaatcg aagaataaat aaaaggaatg aagaaaatat ggcatttgaa
     6241 agcttaactg aacgtctcca gcaggcgatg ggaaaaataa gaaaaaaagg aaaagtatca
     6301 gaagctgacg taaaagaaat gatgcgggaa atccgccttg ccttactaga agccgatgtt
     6361 aacttgcaag tagtcaaaga ttttactaag cgcgtacgcg aacgtgcggt aggggcagaa
     6421 gtgctggaaa gtttatcacc tgcacaacaa atcgtaaaaa tcgttgatga agaattgacg
     6481 atcacacttg gttctgaaac agcagagtta aataaatcac caaaaatacc aactgttatc
     6541 atgatggctg gtcttcaagg ggcaggtaaa acgaccttta ctggtaaatt agccaattat
     6601 ttaaagaaaa atgaaaatgc tcgtccgctc ctaatcgcag gggacgtata tcgaccagct
     6661 gcgatcgacc aattgaaagt attaggtcaa cagctagacg ttcctgtatt tgatatggga
     6721 actgaagtca gcccggtaga gatcgtccgt caagggatgg agttagcaaa agaaaagaaa
     6781 aacgactatg tattgatcga tacggcagga cgtctgcata tcgatgaaac attgatggat
     6841 gagttgaaac aaatcaaaga attgacgcaa ccaaatgaga ttttgcttgt tgtcgatgcc
     6901 atgactggtc aagatgccgt caacgtagct gatagcttca accagcaatt aggtattaca
     6961 ggggtcgtca tcactaaact tgatggggat actcgaggcg gggcagcact atcgatccgc
     7021 tcagtgacag gcgcaccaat caaattcatc ggttctggtg aaaaattgac tgacttagaa
     7081 gtattccacc cagatcgtat ggctagccgt atccttggta tgggggatat gctgacattg
     7141 attgaaaaag cgcagcaaga ctacgacgag aaaaaagcgg aagaacttgc acagaaaatg
     7201 cgggaaaatt cattcgattt caatgacttt attgaacagc ttgatcaagt gatgggtatg
     7261 ggcccactgg aagatctaat caaaatgatc ccaggaatga atcaagtccc tggaatcgaa
     7321 aacgtcaagg tagatccaaa agatgttgaa cgcaaaaaag cgatggttta ttctatgacg
     7381 ccagcagaaa gagcaaatcc tgatctgttg aacccaagcc gtcgtcgccg aattgctgcc
     7441 ggttctggaa acagtgttgt tgaagtgaat cgaatgatca aacaattcaa ggaatcaaga
     7501 aaaatgatgc agcaaatgtc caaaggggat atgaacatcc caggtatgga ccaaatgttt
     7561 ggttcaggcg tcaaaggaaa actaggcaaa atggcgatga atcgaatgat caagaaaaat
     7621 aagaagaaaa agaaaaaacg gaaataagat gaaaaaaaca gcttcggtac agactgaaaa
     7681 tgtctactgc cggagctttt ctatcagcaa tttttattct ttactaatac aattactcta
     7741 aacgaagagg tggcacagat gagatgtccg tggagtgaac aaacagaaag tatgcggcaa
     7801 tatcatgata ctgtctgggg tgttcctgaa tatgatgacc aaaagctgtt ccgaaaattg
     7861 atgcttgata ttaatcaagc cggattgagc tggcagacga ttttaaataa aagtgaagca
     7921 ttcgatgaag catatgatcg attcgatatc aaaaaagtcg caaattatgg agaaacaaaa
     7981 atcagtgaac taatggaaaa caaaggaatt attcgtaacc gaagaaaaat cgaagcagcc
     8041 gtccaaaacg ccaaagcagt cttgaaaatt caagaagaaa tcggcagttt ttcagagtat
     8101 ctttggtcgt tttctaatgg aaaaatcgtt gattcttctt atcattcaca agaagaagtc
     8161 ccaacaacaa gtcctctttc agatgaatta gcaaaagatc taaagaaaag aggatttaaa
     8221 tttatcggaa gcacaacaat ctatgctttt ttagaagctg tcgggatcat caatgatcac
     8281 cttgacagtt gtttccgcaa agaagaggtc gtacgtatcg gcagaaaggg cgaataaagc
     8341 atgcgaaaaa tcatatttta tggtgctata agtttagatg gctatttggc aacgaaaacg
     8401 gatgatcttc aatggctttt tgacactcca acaggtaagc aaactactta tgaagctttt
     8461 tatgaaacaa tcgatacgac catcatgggc agaaaaactt atcaggaagc aaaaaaatat
     8521 ttggagaccg aagcaattta tccagagaaa aagaatttcg ttttttccac ccacgcacaa
     8581 ttacaattgc cagatgctga ggtcgtaaac gaagatcctg ttgcttttct aaaaaatcta
     8641 caaatgacag aaggaaaaga tatctgggtt gtgggtggcg gtcgattgat caaaccgcta
     8701 ttggaacatc aaatgattga tgaatggtat atccagatta cccctgtatt attaggggac
     8761 gggattcctt tattccaaaa aggaacatat gaagcgcgtt ttgaaatgct agacacgaca
     8821 cgattcggtg aatttatcga attgcattat gtacgaaagt agaagtaaat aaaataaaaa
     8881 aggcaactat atgcagaaat gtcctgtaca tagttgcctt ttttattacc gttatgcttt
     8941 ttactctgtt aatttgttag aaaaatctag atacgcatct aaaatcgttt ggaagaaact
     9001 taatgttcct tcaacgatat taccattttc atcaattaga ttcgcaacgt ttccgatata
     9061 tgcttccggt tgctgcaagg ttggcacatt caaaaagaca agtgattggc gtagatggtg
     9121 gtttgcgcca aagccgccga tcccacctgg tgatgcagta acgactaaac ctggtttatg
     9181 atcccaaaca ctgtgtccat aaggtcttga gcctacatct aaagcatttt tcaatactgc
     9241 tggcacagaa cggttgtatt caggagtaac gaaaacgaca ccgtctagtc ccctcacttc
     9301 ctcacgaaaa cgagtccatt cagcaggagc ctgttctgga gtttcgagat cctcattgta
     9361 aaatggcaga tcatcgattt ttacaaatac actttcgtac ccttcaggaa gcaatttcgc
     9421 aaaagcttct gctacttttt tgttgtaaga atcttttctt aaactaccaa taaaaaatcc
     9481 aatttttttt gtcatacgat ttttcctcct tcaaactatc tataataatc ttacaaaaga
     9541 taagtaactg gagcaaataa aaacacttac tgacaaaagc tacatagaag atagattact
     9601 tatttttaac aaaaaagaag tctaaaatct tctattttct aaaaaaaatc gaatttaatc
     9661 aagctgtaaa gaaaaaaagc tttacagggt aaaaaaaaac tgttacacta catcttgtga
     9721 ataaaacaat ggaggtggaa ataaataatg gcagttaaaa tccgtttaaa acgtatgggt
     9781 tctaaaaaga gtccgtttta ccgtatcgtc gtagctgatt cacgttctcc tcgtgatgga
     9841 cgtttcatcg aaactgtagg tacttacaat cctttgaaag accctgcaga agtagtttta
     9901 aaagaagact tagttcttga ctggttatca aaaggtgcac aaccttctga cactgtacgt
     9961 aacatccttt caaaagaagg cgttatgcaa aaacaccacg aagctaaatt ctcaaagaaa
    10021 taaggtgacc ggatatgaaa gatttaagcg acttagtctt aacaatcgtt cgtccgctag
    10081 tcacttatcc tgatcaagtc aagttagacg tcgtagagtc gaaagacttt tacgagtaca
    10141 atctaacagt ggctcctgaa gatattggcc gtatcattgg aaaacaaggg cgtgttgcta
    10201 aagcaatccg cacaatcgtt tacggtgttc ggatcaatgc accgaaaaaa gtgcgtttga
    10261 atatcatcga tgataaggaa aagtaaataa acgtgctgta ctcgaatttt cgagtgcagc
    10321 ttttttgttt tttttattaa ttgaagaaat gattatttgg caaaacaagc aagaaagcgt
    10381 atccttatag gaggagggag tgaacagcca atgagaaaga aaatgttatt tattccattt
    10441 gctctattgt taagcgcttg tagttcggtt actcaagaat cagacaatac acatctatca
    10501 agcagtatat ctgaaactgt taccagtcca gaaaaaagta gtaccaaaac ggcttccacc
    10561 actgaacaaa caatggctac ctcaaataat gagaaaaaga ctgctttaga ccagttgaaa
    10621 gaacaacagc caaatgttcc catgcctcta gatgtaccag tctcttcagg ctatctcaat
    10681 atcgcagcga cacatacaaa gcaaggttat tctattttat attacagaac agatcgtccc
    10741 ctcgggttga atgctgacga attgaatcaa gaaacgccta ttgcaaccta tctatatcaa
    10801 tatgggtttg cttctagtca agaaacaatc caagttttgc agccatttga gatcgacaca
    10861 aatggtcagc aagtggactt aggttctcga atcacagggt atcagcaagg tgcagcaggt
    10921 tcaagttttc ttgaatggca agaaggaaac tggcgtattc gaatccgtgg gaataatata
    10981 gaaggccaag atcccttact attagctaaa gaaatcgtag cttatttgga agaaaacagt
    11041 ctaccagcac ctgaacaatt tggtaaaatt actgttgata tgggtgatac gacgaatcga
    11101 gccgtagaag taagctggca agaaccgaaa aatgcctata cgatcactca tcaagatcct
    11161 atgtctgcgt tgaagatggc agtttctatg aagaggttgt gaaatcagcg cgctgatctg
    11221 agcccatcat ttaactccaa gtcacaggaa gtaatcaaaa atagcacgcc gcatttttgc
    11281 ataattttgg cttaatacct taaaagaaaa ctagaattgc ctcttcacac atgctaaaat
    11341 aaaacagaca aaaaagaaat ggggatataa gttgactgaa tacttgaatg taggaaaaat
    11401 cgtaaacaca caaggtatca aaggcgaagt ccgtgttatc tcaacaacgg atttcccaga
    11461 agaacgttac aaaaaaggaa cggtgctaac actattccaa gacggaaaag cacctgtgga
    11521 attaacagtc aaaagtcatc gcaaacataa aaattttgac ttattgagtt ttgaaggaca
    11581 tccttctatc aatgatgtgg aaaaatatcg ggatggcata ttaaaagtgt caaaagacaa
    11641 ctctgtagat ctagctgaaa atgaattcta ttaccatgag atcatcggtc tgcatgtatt
    11701 agaagatgga actgagttag gtaaagtaaa agaaatcttg tcaccaggtg ccaatgatgt
    11761 ttgggtcgtt caacgtccga aaaaagctga tatattgctt ccatatatcg cttctgtcgt
    11821 caaagaagtc gatttagaag ctggagtggt ccgtgtagaa attccagaag gactgattga
    11881 cgatgaagat tgatgtattg actttatttc caagaatgtt tgaaggtcct atgggggaat
    11941 ccatcatcgg taaggctgta gataaaggat tattagatat caatatctct aacttccgcg
    12001 attattcaga taacaaacat cagacagtag atgattatcc atacggcggt ggagcaggta
    12061 tgctgctcaa agtccagccc atctatgata acatccaagc cattgaaaaa gctgcaccgg
    12121 atgtcaaaaa aagagtaatt cttcttgacc cagctggtaa acgattcgat cagaaaatgg
    12181 cagaagaatt ttcacaagaa gaacacttag tattcatttg tggtcattat gagggatatg
    12241 atgaacgtat ccgcacatta gtgacagatg aagtttcctt aggagattat gtccttactg
    12301 ggggagaatt aggtgcgatg gtcatgatcg atgcaacagt tcggcttttg ccagatgtgt
    12361 tagggaatca gacttcagct caaacggatt cttattcaac cggtttattg gaacatcccc
    12421 aatatactcg cccggcagaa tataaaggga tgaaagtacc agaagtattg acaaatggaa
    12481 atcataaatt gattgaagaa tggcaattaa aagaatcact aagaaggacc tatcaacgtc
    12541 ggcctgatct tttggaaaca cttgaatgga cacctcaaat gacgaaattt ttagaagaaa
    12601 tcaagaaaga agaacaagaa aaaacagcag atgaagccaa gtgacttttt ctaaaatctt
    12661 ctttacagta ggttgataat atgttagtat tttatggtga gtgcaaaagc acttaactat
    12721 tacgatattc cgctgtgaga aaagctcata agaatatttg gaataaggag aagaaaacca
    12781 tgaatccatt aatcgaagaa ttaacaaaag aacaattacg ttctgacatc ccagcgttcc
    12841 gccctggtga cactgtgcgt gttcacgcaa aagttgttga aggtactcgt gaacgtatcc
    12901 agttatttga aggtgttgtt atcaaacgcc gcggtgctgg aatcagcgaa acttatacag
    12961 tacgtaaagt ttctaacgga gtgggcgttg aacgtacatt cccattgcat acaccacgtg
    13021 ttgcaaaaat cgaagtagtt cgctacggta aagtacgtcg tgcaaaattg tactacctac
    13081 gtgcattaca cggaaaagct gctcgtatca aagaaatccg tcgttaatca tcgttatcag
    13141 actgagaaag aacccttggt attcaagggt tctttttttg ttctctgttt ttttatatta
    13201 acgatttcta tttgggacat ttttgattaa taaaaagggg caaaaaaggg gcaacaagtt
    13261 acagtttttc tatatcttct ttcaattttt cttttactcc ctttgtcaca tgcaaataaa
    13321 tcttttcagt gatatcacta ttttcgtgcc caacgcgatc ctgaatagca tacagaggag
    13381 ttcctagttc cgctaatttt gaaatatggg tatgtctaaa aatatgtgaa ctaagttttt
    13441 tatcaatctt catgtcagct ttatgatttc tcaaataggt gttaattgct gttagttgga
    13501 aaggagttcc tttcgttgtt tgaaatagaa attgaccatt cggatttaat tctagaagct
    13561 cattgtaaat agctatcgct ttttttggta aatcaatttc tcgcattcca gcagcagttt
    13621 ttgtagaatc agattttttc atatcagcta ttgaacgttc tctatacatc atcgtcccat
    13681 ttataactac tgatgcatta ttattggtaa tgtgtacatc gtctttactt aatgcgattg
    13741 cttctccagg cctcattcca gtcaaatata gccactggaa aaggagagaa tatcttttat
    13801 tgtgtgaagt tgtgaaattt actagtcttt tatattcatc atcttctaaa aacttatctt
    13861 taatttttat cgtttttgac tctcgtttat aatcaataac tacttcgttg ataggatttt
    13921 tttctacata gccttttttc atagcgtatg aaaaaagaag attcaatttg gatttaatta
    13981 cactgacgta cttatttgac aagtcatctt tatatatcat atcttcaaaa atattattta
    14041 aatctactgt attaatacca gaaacgatgt aagtctcagg tatctttttc tttatagtgt
    14101 ttaagatatt attcgtagga taatatgtag actccttaac ttgtcttttg taaataacta
    14161 accattcttc gacaagctca tgaaatgtta gatcaggctt ctgaagtgtt ttttcgttta
    14221 attttatatc aattttttta tttaactcta gcaatgccac tttctgagtt tctcgagatt
    14281 tatttttata agtaatgctc actttttttc tttttctagt ttttggatca atatatcgct
    14341 cattatattt atatactttt tttccgtttt tatcagtttt ggtttcaatc cacattgttt
    14401 tcatctccta tttgttgcta taataggaat ggataagtaa gccaattata gcaggtttat
    14461 ttttcatcac gtccacaaac tttggcgagg gagggggcgt gtttttttgt ttagtagcta
    14521 cctttccatg aagtatctgc gaagaacact tcaaatgttg ttccatcatt tttcacttca
    14581 aaatatgctg ttccttgtgc ggatttaccc ggttggatgg tttccgaaaa gtaatctcta
    14641 ctagctaaat cagatttaat atctgatgaa tcataaaatt ctaaataact tgaacttgca
    14701 tcaaaaggtg tagtccctgt gttttctaca agaaaatcta cctttgcgta aaataggcct
    14761 tctggtttat agaaatcatc accgtaatct tttgatacag actggacagt tacgttaata
    14821 gagttaccat tttcagaagt aaaaggaact gcttctccta tactatgtgc aactgatgcc
    14881 gtctggggtt ctgaagacga agttgtagtt gtagttgtag tagagcttga atctttggat
    14941 tttccatctt tatctaagaa agttcgttga ttataatata caacctgttc agtttttgga
    15001 ctaacccaaa ttaacattgt ttcatcatca gaatcagttg aataagtata ttggagcatc
    15061 tctaattttt ttatatcttt tgcactattt ccatgttcaa ttaattcaga gtgctcttta
    15121 taataatctc cttgtgcatc ctttgaaata gatacaatta attcatcccg tgtgatagca
    15181 ttgtctaatt ctttgtaaac aaaatcattt gtccatttac gtaacggtga accaagtgtc
    15241 tgataaactt gagttaaatc atcaccaact tttattttat gaacgtctga tgaagttagt
    15301 ttttttgttt catattcaga tgttgatgaa acagtatttt ctttagtgct agcagaatta
    15361 ggactagtgg agcatccgct taataccaag cctaaaaaaa gcaacccaaa aacaattttt
    15421 ttcattttta attcctcact tctatgatat gatttttttg taggatctta gaaataaggt
    15481 ttttagtccg tgttgcagca cggacttttt ttacattata taagaaagcg ataagcgctt
    15541 tctggaagtc cgtaaagatt ctttaattcc tcaattcgtt taggatattg atcattgtct
    15601 tctttgtata gagaaacaat gagattagca gcaaagcaat tagcttcgct ttcagatttg
    15661 cttctagatg ttcttgttga tacatagtaa ctggataagc cacgatgaaa aatagcgtga
    15721 cctaattcgt gagcgcaaat gtagaatctt tcctcagagt ctctcagttc atcatttaag
    15781 aagattattg cacgacctct aatttcttga aactgtcctt tgggattctc gataaaagga
    15841 acgtattgaa ttttaatgcc catcttttca caaatataaa aaggattagc ggactggtat
    15901 ttccgcttca actcctcgac taaattaatc gtatccatct ccataagctc acatcttttt
    15961 gcctttttct ttgtcttctt tcacaatatc ccagaaggtt ccaataagga tatcttttac
    16021 gcgttggacc tgttcaggcg tcagtgtttc tccgccataa gacatattga catttgaatc
    16081 tagtaattta tcaagttcaa ctacttcatc ttttgttgcc cagtcaggaa cttggtttct
    16141 accgagtaaa taatcagtag ttacattaaa ataatctgct acaagttgta gtcttttagt
    16201 actaggagtt ctatttttcc attgataaat cgtgttttta ggtatattta attcttcttc
    16261 caattgagtt atacttatgc ctcttttatg agctaattcc tttattctat ctagtaaatt
    16321 cattttcgca cctcacaagc tacgaaaaca ctaataaaaa aattagttaa aggtgttgac
    16381 aactaataaa agtgttagta tactgttttc gtaagctaaa atattagcta aaaagacttg
    16441 ataactaata aacacttcac ggtcggcaaa ctctgaaatg taaattacta ggcatttctg
    16501 tgtcttattt agctatgtct atatactaat aaaaatatta gttattgtca acgaatatta
    16561 gctaattttt tagcttacag attatttttt tagaaaggag ctatttttat gtctgagaat
    16621 ttagacttaa aaattcgagc ggagatgaga aaaagaagaa tgactttcaa agaactagct
    16681 gcgcttatcg gtatttcagg agcttattta tcagatattc taaacggcaa tcgtgatgga
    16741 aagaaagcac aacagcatat cgaaacagtg aaaaaaatat tggacatccg ataggggggt
    16801 aggcgatgac aaaactaaaa aaacaagatt ttgtaaaaaa atacaattat tctccatcta
    16861 cttatcaacg tcgaatgtcg gaactaaaaa atacagcaat tttctcagcg gcgtatgaac
    16921 gggtcacagg acaagaagtt tggatcaata cagaattata cgataaattt ttgtctttca
    16981 aatcctataa caggttacgc acaagaaagg taacacctaa agaatttatc gagaagcatt
    17041 tagttgattt ataaaaaaca gaacattttg agaggtgaag gttaatgggt aaattcaaca
    17101 gagcattggt attcagcgca ccgctaatca tctacgcttt aggactttgg ggaagcaggc
    17161 aagcgttgat aggaacgatt gtttacatgg tttggatttt tatggggctt gatgaagctg
    17221 aagctgagta cagagcgaaa aagccaaccg agggggctga ctaaaaatga atagaaaaga
    17281 gaaactagaa tgtatattac tattactcag tttaattctt tcactaattt ctctattggg
    17341 aagtttgtat ttttgatatc aacaattttg tggatttggg ttctatttag agtaaatatc
    17401 atttttgttt ggtgattaag gagtttatta aaattaattt tccctacttc aaatgcaagg
    17461 taacaagata tagcactttt aggaggaata tttagcggta cagcttcaga gtatattcta
    17521 ttatcatcga ctgatcctcc tttagcaatt aatttcttaa atggagtagc tgaccatctg
    17581 cggtttaagt tagcggatga aagttctaaa ttaactaaca tagctggttc tgttgaatag
    17641 ttagaaatga ttacttttgt ataaatcata tctgcagcga agtaagaagc attaagttca
    17701 acttctagtt gagggcgcat tccttttatt tttatccctg ttaaaaacgt actcaatata
    17761 aaacttatta ttgacatcca ttgaaaaatt gttaagtgaa aaaaattcaa aataaacacc
    17821 accagttttt aactaaatta taccaaaaag gagagaagaa ataatgcaag aattagtaat
    17881 tttgaaaaat aaagaagctg tgactacgag cttacaagtg gcagaaagct tcgagaaaaa
    17941 acatcaacat gttttaagag atattgatgc actaaaaaaa gatgtgtcca attttggaca
    18001 gatgtttgta gaaggtaatg aaccagattc atatggcaga aatcgacgag ttttcttcat
    18061 tagtagagat ggatttttct tgctggctat ggggtttaca ggaaagaaag ctatctactt
    18121 caaacaaaaa tacattgaag cattcaacga aatggaagat gttattcgca agaatactgt
    18181 tcctcaaaca attgaagata tgatgatcta tcaattagaa gaaatgaaag atgttaaaaa
    18241 agatgtttcc atgcttaaag atactatgcg aattagcgga caacaagagt ttgaaattaa
    18301 gcaaaaagga aatatgaaag ttatgggagt tctaggggga aaagaaagcc gagcttatga
    18361 agaaatcagc aaaaaagtat tctcaaaatt ttggtctgaa tttaaacgta ccttttcaat
    18421 cccaagatat ggcgagttac ctcgtaagag attcgatgat gctgtttcat ttattgaaat
    18481 gtggttgcca gaaactgcga tccgtatgga aattgatcaa ctgaacagac aacagagact
    18541 ttttggtgat gacaatgaat agagctgaag cgctaagaat agggacggca attgctaatt
    18601 gctggtggaa atactataaa ccaatcatcc taagccaaca acatattgac aagcaaaaag
    18661 catggcaaca aataaaaaag tgactccgcc ggcaagcaaa gagtcacaaa gaaaacacat
    18721 cataaggaga ttttagcata tggaaaaaga actttccact ctagatcaat atttgattga
    18781 tcctgattgg ggcaagccga aaattgagga aacaagtggt cgaaaaatca gacgaaatct
    18841 tttgacgaat gaagaactag cttgggatca agatgattta ggcaaccatg taactatttg
    18901 ggatcatgtt tatcttatcc atctatcgaa gcattcgaat aaacctgaat atatttacgt
    18961 catcgaagat ggcttgattg atgcgctaga agagtacgac agagataact tgattgatat
    19021 ctcttattac ggaccaggta agaaatacat tgctgaaatg gaggcagaat ttgatgagtg
    19081 aaatcaaagg gacaacgaac tttgaaaaac tttttagtcg taagttaaat aaaattctca
    19141 agaaaaaagg aaattttgat tatttatctt gggctcacgc gtgggagatt atgaaaaaga
    19201 atgatccaca ggcaacggta actattaatg agtacaaaca ctacagggtt gtttctggaa
    19261 ctcatcaaga ctttcttgtt gaggaatata aaccttttct tatggatgaa actgggactt
    19321 atgtatctgt ctcagtaacg gttaaaggac acacggaaac cga
//
LOCUS       DAYYCY010000037        19126 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000037 DAYYCY010000000
VERSION     DAYYCY010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19126
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.037"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..703)
                     /locus_tag="ACHAZB_001072"
     CDS             complement(<1..703)
                     /locus_tag="ACHAZB_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8613763.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFT"
     gene            complement(717..1641)
                     /locus_tag="ACHAZB_001073"
                     /pseudo
     CDS             complement(717..1641)
                     /locus_tag="ACHAZB_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            1758..2837
                     /locus_tag="ACHAZB_001074"
     CDS             1758..2837
                     /locus_tag="ACHAZB_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFM8613764.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            2834..3462
                     /locus_tag="ACHAZB_001075"
                     /pseudo
     CDS             2834..3462
                     /locus_tag="ACHAZB_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(3572..3799)
                     /locus_tag="ACHAZB_001076"
     CDS             complement(3572..3799)
                     /locus_tag="ACHAZB_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8613765.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(3902..6490)
                     /locus_tag="ACHAZB_001077"
     CDS             complement(3902..6490)
                     /locus_tag="ACHAZB_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HFM8613766.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(6510..7169)
                     /locus_tag="ACHAZB_001078"
     CDS             complement(6510..7169)
                     /locus_tag="ACHAZB_001078"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFM8613767.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(7218..7721)
                     /gene="trmL"
                     /locus_tag="ACHAZB_001079"
     CDS             complement(7218..7721)
                     /gene="trmL"
                     /locus_tag="ACHAZB_001079"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFM8613768.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(7942..8808)
                     /locus_tag="ACHAZB_001080"
     CDS             complement(7942..8808)
                     /locus_tag="ACHAZB_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFM8613769.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            8917..9558
                     /locus_tag="ACHAZB_001081"
     CDS             8917..9558
                     /locus_tag="ACHAZB_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFM8613770.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(9620..10981)
                     /gene="mgtE"
                     /locus_tag="ACHAZB_001082"
     CDS             complement(9620..10981)
                     /gene="mgtE"
                     /locus_tag="ACHAZB_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HFM8613771.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(10999..11898)
                     /locus_tag="ACHAZB_001083"
     CDS             complement(10999..11898)
                     /locus_tag="ACHAZB_001083"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFM8613772.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(11900..12697)
                     /locus_tag="ACHAZB_001084"
     CDS             complement(11900..12697)
                     /locus_tag="ACHAZB_001084"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HFM8613773.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(12675..13379)
                     /locus_tag="ACHAZB_001085"
     CDS             complement(12675..13379)
                     /locus_tag="ACHAZB_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HFM8613774.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            13510..14085
                     /locus_tag="ACHAZB_001086"
     CDS             13510..14085
                     /locus_tag="ACHAZB_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HFM8613775.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            14203..14880
                     /locus_tag="ACHAZB_001087"
     CDS             14203..14880
                     /locus_tag="ACHAZB_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP adapter SpxH family protein"
                     /protein_id="HFM8613776.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(14959..16767)
                     /gene="pepF"
                     /locus_tag="ACHAZB_001088"
     CDS             complement(14959..16767)
                     /gene="pepF"
                     /locus_tag="ACHAZB_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HFM8613777.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(16943..17941)
                     /locus_tag="ACHAZB_001089"
     CDS             complement(16943..17941)
                     /locus_tag="ACHAZB_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA family protein"
                     /protein_id="HFM8613778.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(18167..18820)
                     /locus_tag="ACHAZB_001090"
     CDS             complement(18167..18820)
                     /locus_tag="ACHAZB_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HFM8613779.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT         5525 a         4136 c         3324 g         6141 t
ORIGIN      
        1 acgtaaagct tgctgccaaa ttagcgattg aaactaacga ttgctttcta ggaatcaagg
       61 acaagcgaat accatttgac gattgatttg cttgctgatc ctgtacattg cggacccccc
      121 acatcgtccc gtagagaatc gccattccaa tcagagtgaa gaaataaaaa cttttgaaac
      181 tcatattacg acctgcatga acttcttgaa aagatacttc ttgttcaaaa acagtcatga
      241 tgggcgcgag atcaacgttc tccacttctg ccactttact cgaaattata ccaatacgct
      301 gtaaaaattg atccatgacg tttttcatca acgtctgact cattcctgct tgacccacat
      361 ggagcgaaac agatgaacca tcaatgagat aaaaaccagc caaatcatct gcatttagct
      421 tttcattcgc ttctacttcc gatagttcct gtacttcaaa taatggttta tcattgactt
      481 cgacttgttc taagactgtg gtaaaatttt ccgaaagcgc tgtttgatcc gctttgatta
      541 caattccagt attaatcgtt tcgagattgc caccttgatc aatctcgcca aaggccgcca
      601 taaacatcaa tcctaaaaga attggaaagg ccaatgtcca aaacaacatg gatttatttc
      661 gcagcagtat ttttgtgcga tatttataaa aatgccaaaa cattatttcc cctcctctag
      721 tctctcaaat ccttgccagt aatctctaaa aagacatcat tcaaggacgc ttctttggtt
      781 gctatttttc cgtaactgat cttttcttct tgtaagattg taagaatcgg caacaatact
      841 gtatttagtt cagtagttgt tacttggagc tgaccttttt tataagcgat tccatcagaa
      901 gctattgttc gctgacaaca cgttttctgg gagtaatggg atgtcgatcg tcaaacgatc
      961 cgccgtccgt accatatctt ttagagattc tttggttcct ttagcaatca cctgtccttg
     1021 atccatgatc acgatctggt cgcaaagaat ttctacttct tccatgtaat gtgttgtata
     1081 gacaatggtt gctccttgtc gattcagctc cttgatactt tctaaaattt tattccgact
     1141 ttgggggtct acagctactg tcggctcatc taaaaaaatt aaccgcggct tgtgggcaat
     1201 cccacatgcg atgttcaacc gacgtttcaa gccaccacta agttctcctg gacgaaacga
     1261 aacatatttt tctaatccca ctaattgaat cacctcttca atcaaggctt tccttttctc
     1321 tttttcggaa acatataaac cgcaaaaata atcgatattt tcttgcacac ttagttcgtc
     1381 aaagaccgca acgtcttgag gaacgacccc aatcattgcc ttgacagcat aattttcagg
     1441 agtcattgtt gtcccgaaca atttaatggt tcctttgtca taatttaaca aagaaagcat
     1501 acagttgatg gcagtcgatt tcccactgcc gtttggtcct aacagaccta agacttctcc
     1561 ttcttgtact tgcagatcaa aatgattcaa tgctacaaaa tctccatagc gtttgactaa
     1621 tttatcaatt tcgataatca tcatgaaaaa ctccttccaa atttcttgtt gatctgacta
     1681 taataaagtg caagaaaaga aactagtgac gattggctaa accacacatg acatttgtca
     1741 caatgaagga gtctgctatg cataaatctc ggctactcaa cgaaactagc tttctactgg
     1801 ttatctcatt gcttttgctg gtaagtcaag gattcgttgc tatgcaagtt atctatttat
     1861 tgatcgccgt tttattcact ggtgtcagtt atttactgcc tacaaagcca aattggattt
     1921 tccaagttat tctatttctt ttatgtgcct ttttacctga tcttgtcctg tttctgccag
     1981 cgacgatgcg agtgatcgcc cctgaagaaa gccaccttga ttacttgttt ttgttaggtt
     2041 ttttgttgat ttcgacggtt ggtaatcatc cccccatcaa ggtcgccttt gtcgtaattt
     2101 tgctgttttt ttccttttac ttacgaaaaa aagatcaacg aacgaccgat cttgagaaaa
     2161 agtacattgt ctccaaagat actcattggg aaaaacaaca agagttatcg gaacaaaata
     2221 aagcattggc cctgtcacaa gaaacacaat tagccttgca aattgtggaa gaacggaacc
     2281 gaattgcccg agacatccat gacaatgtcg gtcatctgct gtcaagtgcg attttacaaa
     2341 ttggtgcgct tgaaatgatc aatcaagaac caaaactaaa agaaccatta gaaaatttaa
     2401 gtgccaccgt tcacactggg atggatcgga ttcgtcaaag tgtccatgac ctccatgaga
     2461 catcgatctc ttttcaacac tccttagagt ttctgatcgc tgattttcct tcttctgtta
     2521 cgattgaagg aaacttgttt gaaggattga atgaaacgca gcaaaactgc ttcttaatgg
     2581 tcatcaaaga agccttaacc aacagtatca aacatagtca tgccaaaacc gttgttttac
     2641 attttcgaac gttgccggcc ttttatcgct tgcaaatcac gaatgatggc accagcccac
     2701 aaaaaaattc tgctcccggt attggtttga aaacgatgcg tcaacgtgtt caagaacaaa
     2761 agggacaagt ccatattttc caagaaaacc agcaatttca gttaacgatc attttaccaa
     2821 gggagggaca ccaatgataa cagtagtcat tattgagatg atccatttgt gacgaaatcg
     2881 ttacaaacca ttttagaaag cacgaaagag atccgtgtgt taggcattgg tcattgtgcc
     2941 aaggatgcgc tagtcttata cgaaacccat cgtcccgatg ttttgctgac ggacatccgc
     3001 atgcctaaac aaacgggcat cgatgctgcc aaagaaattt tggctaaatt tcccaaagcc
     3061 atccttttgc ttttgacgac attcaaagat gaagaataca tccgcgaagc attttccatc
     3121 ggtgtcaaag ggtatttaat caaacagaac ttgcaagcga ttattccttc tgtcaaatcc
     3181 gcttataatg gacaggccgt atttggtaat gaaatcatcg agactttcac tcaaatgatg
     3241 aagaataaac cgacgattga tagtcatgct tatgcttctt ttagtgaacg tgagcttgca
     3301 atcatcaaag aagtagctgc cgggaaaaac aataaagaaa ttgcagatgc cttgtacttg
     3361 agtgacggca ctgttcgcaa ttacatcagc caattactag aaaagctaga tttacgggat
     3421 cgcacgcaat tggctatcta tttttatcag catttgcaat aagtcttatc taacacaaaa
     3481 aaacctgaat gatgctcctt cgcatcattc aggttttttt cacatcttct attcaattgt
     3541 cccgcaaacc atttcctcac gattgaaaga atcaaggatt cgcagggctg ccaggattcg
     3601 ttggattgtt cggcgtgtta ggcggaaaaa taggcgcctt gtctaacgtt ttttctagtt
     3661 ttgattcttc acgatcgtct cgttctacat caggactttc attcaatttg aattcttttt
     3721 ccatctcatc taacttttta aagtcattgc cagattcttt tgctaatttc gctaatttat
     3781 cttcggtttc tttagccata aagtaacgcc ccccttttga tttcagctta gcatatcaca
     3841 ctgaaaaaac aaaagaaaac gctaaccaac gatcattttt agctatttct catctttaaa
     3901 atcaagcatt ttttgccggt ataaaatcgt agggagaact gttttccata ccaatcatcg
     3961 gatctactaa ctggttatcg atcacttctg ctgtaagaac gaaatcttta gaaacagcag
     4021 gttctacctc ttctttcttt tcttctccaa caaatgaaga aaacagatct ggttccactg
     4081 tttccattac ttggtttttc gtttcattcg tcccactgtc atcttctaca ggtagctttt
     4141 cttttacaac ccgtggctct tttgtatagt tcccatcttc aaatggatca tttccgacca
     4201 aagaatcttc ataagctgct agtttggtcc ggagagtgat attcatttta tcattatcca
     4261 tgatccttga tttcaacgta gtcatccggc ttgctgattc attcgctaca caagtgacat
     4321 aggcttgctc ttcccctaac aaaatcaaca gttccttact gcgagtcact gccgtgtaga
     4381 gcaaattacg ctgaagcatc cgctgatatt gatggaccat gggcaaaata accatcttga
     4441 attcactgcc ttgtgattta tggattgagc aacaataaga aagagtgatt ttattccatt
     4501 cgtttctttt ataagtgacc tcatttgcat caaactggat aactaattca tccactttat
     4561 cttcagaatc ttttgccaag atgatacccg tgatttctcc catatctcca ttgaatacat
     4621 tcaattcggg agtattgact aattggagga ctttgtctcc tatccgataa acggtgtcgt
     4681 tccaatgtac ttcctttttt ccaggtttcc ctggattgaa tatctcctgc atcattttat
     4741 ttaacgcatc gatccctgca ggtccccgat acattggtgc taatacttgg atatcttgtg
     4801 cacggaatcc cttatttttc gctttttcca caattttaga gatcaagggt tcaatttgat
     4861 aagcatcact tcggaaatag gaacgatctt tttgattctt tgtgaaatct tttggcagac
     4921 gtccttcttt gatctcatgg gcaagaggaa taatactaga accatcacct tgccgataaa
     4981 tctgattgag ttccattttg ggaatttgtt cgatttccaa aagatcatgg agtacttgac
     5041 caggaccgac agagggaagc tgatctttgt ctcctactaa gataacttgc atattcgtcg
     5101 gaatcgcttt gaataatgtg ttagccagcc atgtatcgac catagacatc tcatcaacaa
     5161 tcaaaagtcc gccttctaat tctttcgtac tgactgaagg tgttttttcc cgtccattca
     5221 gccccagaag acgatggatc gtactggcag gcagacctgt cgtttcgttc attcgtttag
     5281 ctgctcggcc agttggtgct gcaagtagaa taggaaaaat ttcttgggta tattttgaag
     5341 gatccaattc cagtccgttc aactctgcaa aaagattgac gattccgttg atcacagtag
     5401 ttttccccgt acctggtcct cctgttaaaa taaatagagg ggaacggatt gcttcagcga
     5461 tagcttgttc ttgcgaaggt ccatactgta tgccgaatat tttttcgata cgccgaatat
     5521 tcttctgcac ttctttttca ggataatgga tttctttttt acgactgaga aggcgctgga
     5581 tcgaattacc gatcccccat tcagaaaagt acaaactgtt ttcgtaaaga ttagtctctt
     5641 cttgttgtat ctttccttct tcaacaagtt ggataattac agtagcaacc tgatcaggct
     5701 ggatttcaat tgggcgactt gtttctaaaa tacggatcgt ttgctgaagc aatgtctccg
     5761 ccggtacgta tgtatctcct gtttgaactg catgctgaaa aatctgatgc aagatagctg
     5821 ctcgaattct tctcggtgaa tctgctgcaa taccgatttg ttcggctaga ttatctgcac
     5881 gtttgaaccc gattccttct atgtcttcga ctagctggta cggattttct tcgatgatat
     5941 ccaaagactc gttcttatag gcctggtaga tactgaaagc taactgacta ccgaacccgt
     6001 aacgattcag tcccatgatc acttgatcca tcccgtggtt tagccgaatc gtctcagcaa
     6061 gcatttctct ttttggcttc gtcaaaccgg agatttgttt taataccgaa ggatcagcaa
     6121 tgattttttc gatagcatct tctcccagca attctacaat tttttctgct gttttcttcc
     6181 cgattcctgg gaatttttcg ctggaaagat acgctacgat cccatttgca gttgttggct
     6241 tttcttgctc atatctttcg agtttaagct gccttccgta tttaggatga tccactaatt
     6301 ctccataaaa acgataagtc tcattttctt gaatatcgcc gaaacttccg gtgactacaa
     6361 tctctttttc acggtaatca gctgtggttt cactgatgtg taccaaaagc actttgtaaa
     6421 aattactagg attttgaaaa aaaatcgcgg caactgtacc aacgaaataa ggcatttctt
     6481 ctggctgcat cggaagccgc ctccttcttc tatagtaaag gttctggtgc tgtttcagtt
     6541 cctaaaaaat ctgtttggtt ccatactttc agttcatatg gcaataatct gcaaggctct
     6601 tcagtctcta aaatcgtcaa actactattg aacaatccac ccatttcacg caactcactt
     6661 agctcttttc ctgtcatcca ttgaatggct gcagttaaag aagctccatg tcctacgaat
     6721 aaaagtgggc catctttatg ttgggcaacc gcatctgcta caacagtttc gatacgtgtg
     6781 attgccgatt caatcgtttc tccgtgaaaa ggagaaggat catacagatc taaatgatta
     6841 cgcaaattcg gcagattatc cggatatttc tggtacattt catcgatcga ctgaccttct
     6901 aaagtaccca gccctagttc tcttagctta tctgtataaa aaatctctac cggacgagca
     6961 agctctttgt tgataccttc tgctgtatca cgtgctcgct ttgctgtact ggaataaatt
     7021 gctttaaaag gaacatattt tacctttttc ccaaatgctt tgatttcttc gtaactttgt
     7081 ggcaaaagcg gagaatcgcc attgctccct tgaaaacgtc tttcctgatt ccattgtgtc
     7141 ttcccatgtc gggtaaaata tagttccata cgcagcaaac tcctgtcttt cttttatttt
     7201 actctataat aaactgtcta atccagcttg tcattttcat agtgatgcgt caattctaaa
     7261 ttagggaatc cttgctggcg aagtgcttca tacactatca ttgcagctgt attcgataaa
     7321 ttcaatgagc ggacatgctc atcattcata gggatgcgta aacacttttc ttcgttttca
     7381 cgcataaatg tttctggcaa accggttgtt tcttttccaa ataaaaagaa gtgctcttct
     7441 ccatcagcaa aattttcgtc actatatact ttatgggcaa attttgagat caaatgtagt
     7501 ttacgattac ctaaaaatgt taaaaaagct tctaaattac tgtggtaagt aatatttaca
     7561 tcattccaat aatccaaccc tgcccgtttt agatgtttgt catcagttga aaaacctagt
     7621 ggctcgatta aatgaagcgg agtattcgtg gctgcacacg tacgggcaat atttccagtg
     7681 tttgcaggga ttaatggttc aaataaaacg atatgattca tcaggatcat tcctctcata
     7741 tttatgttcg tttctatttt ctgacaattt gatttttgca taaaaaaaaa cgtatcaact
     7801 cggtgtcagt cactgcgagt cgttacgtta gtgaagctct tctcactaac ttttatcaga
     7861 acaggaacct gataaaaacc aatgaaaaaa caacttgttg ctaatataac accctcaact
     7921 tttttttgca acctctactc attaaaaact gctagaaaaa cgttttctct tagttccttt
     7981 taataattct atgtcaattg taatcttctt caaaggattc ttatataagt ctgctttgat
     8041 tcgctcttct acctgccatt ctaaaggtga catggccaaa agatcttttt gattttcctc
     8101 tggtatctca aattcatagg ttacacgctg acgttcagtt aatttcattg cttcagcaaa
     8161 cttggctact acacgttcat tggaataact ctgtttttca ggttgattcg gataaaaagc
     8221 ctgacgtagt tcctttaaat aattttcttg tggcaccacc ttcaacactt ctccatcttc
     8281 tgagagtaca cgttggaatt cttggtaatg agaaggcgaa aagatattta aaaccgcgtt
     8341 gaatgtttga tcagcaaacg gcaaattggt caaatcagct acacaccaaa atgcttcggc
     8401 agattgttct gtagctgcgt agactccttc ttttgaaata tcaaatccaa cagcattttg
     8461 aatctttttc aattttgaaa gttcattcaa gaagctacct tctccgcagc caacatctaa
     8521 tagcgagact tcttcaggta aatgctgcgc aatagtctca ataacgggct gatacatgcc
     8581 agattggatc attctacgcc ttggaacgaa cattccttga tcgtattctg ttttaaccgc
     8641 atgggataaa aaataaagtg ttcctttttt ggaaaggtca aaacggtgat tatttttgca
     8701 tatcaagcca ttatcagact tttttacttc ttcttggcaa accggacagc ggaacagctg
     8761 atgattttct gccaaaaact ggcgtcctcg gtcaattttt tttaacatag ttccccctca
     8821 tttctgtttt atcttaccgt tagatgctca ctttggcaac tgctaaaaat atgatacaat
     8881 gcctagaaga aagtgaaaga taaaggagaa gattagatga tcaaagaatt ttgcgctgaa
     8941 aactacacat ctattcctct agcaattgct agaggagccg atcggatcga attgtgtgac
     9001 aacttagcgg tcggtggcac aaccccgagt actggtgtga tcgaagaaac gttaagttat
     9061 gctggggaaa aagatatccc cgtaatgacg attattcgtc ctagaggggg cgacttcgtc
     9121 tataacgata tcgaattgaa aatcatgcat actgatttga ttgaagcaaa gaaattaggg
     9181 acggatggcg tagtatttgg ttgtcttact ccttcaggct ggctagacga agaggcctta
     9241 gaactattga tcgaaacagc agaagggttg cagatcacct tccatatggc ttttgacagt
     9301 attcctgaag atcggcaatt cgaagcaatc gattggctgg tagaacatgg tgttgaccgt
     9361 atcttgactc atggcggtcc tgctgacaaa actattgtag aaaatttccc acggcttaaa
     9421 gaattgattg cctatacaga tggccgctta atcattttgc ctggtggtgg tgtcactgca
     9481 gagaatgcag cacatgtagc taaagaattg aacgtaagcg aagtacacgg aaccaaaatc
     9541 gtggatcttc agttataatc ttataaacaa aaaaacattt gcaacaggca taatgcttat
     9601 tgcaaatgtt tttgtttttt tacataaaat agcccataaa taaagaagca atattgaaat
     9661 aaatcagtac actcgtcaaa tcgctcaaag tcgtgataaa gggaccgctt gctactgctg
     9721 gatcaaatcc tagacgatcc attagcatcg ggattaaact acctgcaaga ttcgccactg
     9781 tgattgcaaa tagcatagcc atccctatga caaaccccaa gataaaatta tgtttccaaa
     9841 aacctacgat tagaaaaatc gtgatccccg taacagctcc tgtgacaacg ccggtcaaga
     9901 cttcagccaa aacaagtcga acaaatccat gatctttctc atcagaaatc gcaagtcgac
     9961 gaacagccac agctagcgac tgggttccag cattaccggc agttcctgta atcagcgaaa
    10021 tgaagactgc taaaatactc gcttcactta ccaagtcttc atagtgactg attaaagaag
    10081 cggtcgacat acccaaaaac aacagagtga tcagccaagg caaacgtcga gacgctgcct
    10141 ttactggatt ttcattaatt tcttccacat tgaccccggc aagtcctgaa taatcgctag
    10201 cagcttcatc atcaataacg tcgatgatat catcgactgt gacgattcct aacaaatgat
    10261 catcataatc ggtaacagga agagctaaga agtcataatc tctaaatgtt tgtgccacat
    10321 cttcttggtc gtcgcctaca tgaactgaaa gcactcgttc gctcatcaga tcagaaatca
    10381 ttgcgtcgtc atcattcacg attaggtcgc gcagagaaat aacccctact agctgacctt
    10441 cttgattaac gacatagatg taatagattg tttcagctac atctgcttcg ttcttcagca
    10501 cgtacatcgc agagcgaaca gtttgattgg ctacgatcga aacaaattca gttgtcatga
    10561 tcgcgccagc cgtttcatcc tcgtaatgga gaagttcctt gatttcactg gcatcgtctg
    10621 ccgacatcaa acttaaatat ttagcaattt gctttttatc cagcgtatta agcaagtcaa
    10681 cagcattatc tgtatacatc tcagaaagca tatcagctgc atagctagga cgcatttcat
    10741 ctaggtattc tttcatatgt tcgttgtctt cttcaatcac atcgaacata tccgctaatt
    10801 ctttaggcga cagataggaa tagatcagct gcctgttctc ttctgtaaga gattggtaga
    10861 attgcccctg ctcatagata tgcatctcta aaaaactgtt tctgaatgcc tgaatattat
    10921 tttcccttaa atcttgagtc aactgggcaa atctttcctc taattcttga ttttcatcca
    10981 aaccaatctc acctcgcctt attcttcaaa ccgtctcaca actgcctcca tgtctgcggc
    11041 taaaggctgc ttgaactcca gcaactcttc tgtaaatgga tggtaaaaac gcagatcata
    11101 acaatgaagg gcttgtcttt ttattcccag atccattcgc ccgccataca tatcatcacc
    11161 gagcagggca cagccaattg cggaaaaatg aactcttatt tgatgtgtcc ttcctgtatg
    11221 cagctggata tcaactaacg cgatttctgt tagtcgcttc tctatccagt actcagtctg
    11281 tgccgaacgt ccttcatcag agaccattcg cttcaatagc gaagaataat ctcgggcgat
    11341 cggcaaatcg atatgtccat gtgccgataa atctgaaagc cttccactga ctaaggcctg
    11401 atattttttc acaaacttct tttcacgaag ttgcatatct aattttgcgt gcgcaaatcc
    11461 atgctttgca aaaagcatca aaccagatgt atcccgatct agtcgcgtca ccacatggat
    11521 tacttggttt tcgtaacctt gttttttata ataagccttt acacgattag ccattgtccc
    11581 atttggatgg tattgggcag gaatagacga aacgcctgct ggtttattta ctaccagaac
    11641 gtgttcatct tcatacacga tatcgatcgg tgtttcatcc aaaagtacgg tttcatgttc
    11701 tccttcagca ggaatcacaa tggttacctt atcatcctta gctagagaaa acaagacatt
    11761 ttctacttga tcattaactt tgatttgacc gccttgaaat tttattttcg ctaacagacc
    11821 ctttgagatc cctttttctt ttaaaaaata ttttacttgc tgagaatgat ctttctcata
    11881 atcccatgta aactgcatat caatcctcac taataaaggc atctttcaca cgatgccaaa
    11941 aatgcatatg tcgataagaa gcaaaatgga tccgttcttc tgctattcga taataaattg
    12001 agcggatatt tgcttttgcg acattcaatt gatcaaccgt tacaagataa tcttctgtat
    12061 tttccaattt tatttctagc cattcatcat gggcaatcac gatcggagaa cccaaagtac
    12121 gaaacactcg attattcagt gaagcaatct ctgccaactg aaaggcattg atactaggat
    12181 ggatcacagc accgccgaca cttttattat atgctgtcga accagtaggc gtagaaatcg
    12241 tcaatccatc tcctctaaaa ctttcaaaca gctcgttttt gatgtataca tcggctacca
    12301 tcgtacgatt ggcccgttta atcgttgatt cattcagtgc taaaaaatgc tggtccggtg
    12361 tttcatccaa ataactgatc cgaacatcca gcaagggata actaacactt tgttcccggt
    12421 tcgttcgtaa gctgtctact agttcttcca gctcgtaatc ccgccaatcc gtgtaaaacc
    12481 ctaaatgacc tgtatgcacg cccaagaaac gcacatcatt cagacaatga ctgtagtgat
    12541 ggaacgccga caataatgtg ccgtctccgc cgactgagat gaccaattct ggctcatgat
    12601 catctcgttg aatccctgct tgttctaaaa gtgcgttcaa acgttctgtc acttctatag
    12661 tctttgcttc tttattatga acgatcgcta ctttcatccc ctgcctcctc tctttcgctt
    12721 tctttcttca aaagcgttcg atagtagaca tcatctgatt ttcctcttcc atgggagaat
    12781 aaccgctgtg cttcttggat ttcttcacgg atactggaca tctcttcatc caactgatag
    12841 gcagcttctg ccgcacgctg aagtcgttca ctcatttctt ctggaaaaac gccttgatat
    12901 ttataattca gcgaatgctc aatcgttgcc cagaaattca tcgcaagagt ccgaatctgg
    12961 atttccgcca aaattttctt ctcaccgctg atcagctgta caggatactc gatcaccaga
    13021 tgataagaac ggtagccact tgctttttta tttgtgatgt aatctcgttc ttggagaact
    13081 ttcatgtctt ttcttttacg gatgatctca acaacttgat ggatatcttc tacaaactga
    13141 cacatgatcc ttaacccggc aatatcagac atttcttctt ctaaacgtcc cattggaata
    13201 tgccgtaatt gtgattttgt tacaatacta tcgactggct tgactctccc agtgacaaat
    13261 tcaatcggga catgtcgatt ttgttcacgg aactgtttgc ggatcccccg cagtttcact
    13321 ttcaattctg atacagtttg ttcatatggt gctaaaaatg cttcccaatc tcgttccata
    13381 gctgatacct ctacttcatt aacattcatt gtttattcta ccatatatgc aaagtttttt
    13441 cgtactcttg atttatgcat tgaccaaaaa aagaaaatta ggcaaaatag acaaaaagga
    13501 gtcgatcaaa tgacacaaat gcttgaaatc gaatataaat ctatgctgac aaagcaagag
    13561 tacgagatat tcatcgctca ctatcaatta acagataaag atttccgtgt acagactaat
    13621 atttatttcg ataccgttga tgaacagctg aagaaacaaa attgtggcct acggatccgt
    13681 ttcgttggtc atcaagctga atatacatta aaaacacctg ctgaaaaagg gcgtctcgaa
    13741 acaacggata cctttataac agaagaagca gaagcattta tcttcgaaaa acgtctgcct
    13801 cgttcaggcg cagtttttca aaaactttct gatttgaata tcgatcctgc ttcattaatc
    13861 caaaccggaa agcttacaac aaaacgggct gaattcccta ttgaagaagg acttttagct
    13921 attgatgaaa gctggggaga caatctacac gattttgaac tggaactaga ggtaggggat
    13981 gcatctgtgg gaaaaattgc ttttataaat tttttaaaac gtttttcttt accttatcgt
    14041 cctgctaaga acaagatcca acgtatgctt gaagctaaaa attagaaacc tttcattttc
    14101 acaaggcact tgagttcatt tttttacttt tttctgttaa gttataggat gtgaaagaat
    14161 ctagtcacta gttgtataga atctattttg ggggagattc atatgatcga aatctattta
    14221 tttgttaatc ctttagggga atactgtttc gaaatggaac aacagttatt acaatttatc
    14281 gaagaagagt acgggacacc atcaaaagaa aagatgcaat ttcgctttct tcctttggtc
    14341 aatttgcaga caattgggga tgtcatgcaa caaaaaggaa tttctcagaa caatcttgag
    14401 gaacgtaatc acttattctc aatgacttat tctgcagcat tggactgcaa agcggcgcag
    14461 tttcaaggaa agaaaaaagg ccgccgtttt ttgatcaaat tgcaagaagc agtcggctgt
    14521 aatgacatac catattctca agaacttgct gaatccatat ttgaagaaat cggcggcgat
    14581 ttgaatatgt ttaaagaaga tcgtcagtct gacttcgtta aagaagtatt cttttctgat
    14641 caaaaaattg ctcgggaaat gggcatcaca aaacaccctt ctgcagtcgt ttacaattat
    14701 gcctgcgacc gtgattttgg tgttttagta gaagtaaaag gtacaagtag cctttggaga
    14761 atcacagagc tgtgtaaaac aacagataac tcatatcaag tttttaatgg tgaacattta
    14821 catacaaatg attctcagtt ccattcaaga actagccatt tacacttgct ttctaattaa
    14881 aaaaatacgt cgactccctt acaagagagc cgacgtattt ttttcgtttc tattcgagaa
    14941 aatcaaaaat agaaagcctt ataaacgatc gatcaattct tctaattcat ttagacgttg
    15001 ttcaaacatc gacattgcat cttcaatata tgcagcctgt gtcatatcaa ctcctgcttt
    15061 tttcattact tccacaggat aatcgctgtt acctgctttt aggtaagcca agtagttctc
    15121 tagtgcctct ggttcctggt ttagtatttt cttcgccagt gctgaagcag cagaaaagcc
    15181 agtcgagtat tggaaaacat agtaattata ataaaaatgc ggtatccgtg accattcgaa
    15241 tttaatttct gggtcttctt cgactgctgg accataatat tttgcattca atttaccata
    15301 actatcactt aggtattcac tagtcaatgg cacacctttt tcatcttccg tatgcatgaa
    15361 atgctcgaat tcagcgaatt gtgtctggcg gaaaactgtt cctttaaacc catccaagta
    15421 atggttgagt acataagccc gtacacgagg gtctttttct gtttccaata aatactccgt
    15481 taggatattt tcattcgttg tcgaagcaat ttctgctaaa aagatggagt agtcgccgta
    15541 cacataaggc tggttcgaac gagtgaaata actatgaaca ctatgtccca tttcgtggac
    15601 aagcgtaaat agctgatcca gcgtatcatg ccaattcaat aaaatatatg gatttgtgtc
    15661 atagcttccc gaagaataag caccgcttcg tttcccttta ttttcgacaa catcgatcca
    15721 acgttcagag aatgcttttt ctacgatggc catgtattct tcacccattg gtttcagtgc
    15781 ttctaaagct ttttcttttg cttcttcgta cgtaaaggta attggagctt cccctaagac
    15841 cggtgtataa agatcataca tgtgcagttt ttccacttct aataaacgtt tccgtaattc
    15901 catgtatcga tgtaacaaag gcaaatgctt gtttaccacg tctaccaaag tatcgtatac
    15961 actttcagga atatgattat tgctcaaaga cgcttctctg gcagagctgt aattacggac
    16021 tttcgcttta aaattatgtc cttttatatg tgtgcctaaa gtagaagcaa atgtatttct
    16081 aaattgttcg taaacactgt acaatccctt aaatgctgct tcgcgcaccc ttcgatccgt
    16141 gctttctagc aactgaccat acacgccatg agataattgg actatttcac cattttctcc
    16201 ttcaatcgtt ggaaaaacta gatctgcatt attcaaaaca gcaaatgtat cacttgaagc
    16261 atcaaagatt tcacctgctc cagcaagaag agattcctgc tcagcagata agacatgggc
    16321 tcgattatct actatttgct ggatataatg gcgatagact tccaattttg gttcttcttt
    16381 gaaatattgc caaatctggt catctgacaa ttgcaatatt tctggttcaa accaggaaac
    16441 ggcttcacta actttcgaaa acagactgct tgctcttgca taaagcgctt ggtaatctgt
    16501 atttccagtg tcttgatcgt ttttaaggtg cgcatataca taaatgactt cagtttggcg
    16561 ataaaccctc aatacgaatt caatcgcatt taaaaattga gaagcgcctt gatcaagtgt
    16621 tcctttgtgt ttttcagatt gttttaactc ttctgataat tccaaatatt tctcatcaaa
    16681 ctcttggtcg cttgaaaaga tcttggtcaa gtcccaagtt aaattttcag gcaattcttc
    16741 tcgttttggc aactgcttta cttccatgaa atccactcct tttcttccct tagctctatc
    16801 tatcaaaata gatcatagtc taacatccac atgtcacagc atctaacttt tctactcatt
    16861 catgtgtctg cttccatcaa ttttagcata actaaatctt gttttacata gaaaaccgat
    16921 tcctagccta tttttatact tactattttt tcaaagaaga caagtgtatg cattcgttaa
    16981 aaaactgctg atagaccatc tcttcatcaa tcataggaaa aagccattcc cttttatgtt
    17041 cttttatttg gcaaagccat gagttgaaat caggaacttt ctggttttcc aaggcttctt
    17101 cgtacaacat tcgataaaga aaaacctgtt cttgtaaata aaagaaaaac cgactttttt
    17161 gatagatcca cgatgataga tgcaaaagat gtttatgacg caagtagcat tgttcttgta
    17221 tggatactgt attcttttgc ttgtgaataa gttggcgtgt cagccatttc ttcccatctt
    17281 cactcacttt ttctattttt ttcatttggt tcattttaat ttcgttacta gtgaatattt
    17341 ctttaaggtc cctactagaa aaagtccagc agctactgca aaaggaaata tgccctgata
    17401 cagtctctgt aatatgatga tacaaataaa gcttttgttt cttgagatca gctttccagc
    17461 aatgcactcc tctttttcta ttatagtagc agcaactttt ttctatcaat gagaattgcg
    17521 attgtccaag aaaagaatta ccaagtatcc accatacttg gtaactgtta ttcaaataat
    17581 tttgggttct ttcaataaat ctctggtgag acagtctaga acattggatt tctattgcta
    17641 gattcgccaa caaaatatcc ggcctttgcc gcaacctcgg caaataggct tccaaaaata
    17701 ctggttctac tgactgtccc agccaatcca tgaagcattc ctttaactgt aagtgctctt
    17761 ttgattctct ttcactaaag accgaacagt tatctgattt ttgatgggca aaatgagcac
    17821 attttttctt tccctttttc aaaatcactc tttttcgaca gccacgacaa taatatttct
    17881 ttcctttttc tgcatgttta gcaaaacagc acttattata ttcattcatt gcaagcagca
    17941 tttttctccc tctgaactta tttacgtcta agaatttatt tacacgaaaa aagagggcgt
    18001 gaaaaagcta ttctgcatca agtaataaga acagctaaac aaaaatagtt cccaatattt
    18061 ttcgtttggt tgggcttgtt accgcagatg caagcttttg aacacattta tacgaaaaaa
    18121 agactgcgac acaatttttg tcacagtctc ttactataat gctgatttat acaaaataaa
    18181 atttcgttcg ctcgatagca ttacgcgcca tcagacattg tccatattca ttcaatgttt
    18241 ctggtgtacg atcgctcttt tcagcaaact ccaaaattcg agcaatctga ttctctacat
    18301 ccgcttggtt gagattttcc atccagaagt gaacgactag gtaatagcgc tcttcgaata
    18361 aatacaaatc agtccaagca gactctagtt cgacatctgt tgcaagttgg atcacttgtt
    18421 caaaatcttc aaaagcaaaa gttgcttgtc tttcctgttc ttctagggcg cttccagata
    18481 attcttcctc cggatcgctg ccaataatct gctgacgaag aaagtcactc acatcttctg
    18541 aatcaacagt agaatcttct gattgagaaa tttcttcatc cggaagattt ttactgataa
    18601 acagttctaa tccgtctcct tttggcaaaa cttggaaagt gacagcttcg cttcctcgga
    18661 actcctcttc gatatcaact tcttccaaaa tactataaaa gaaattttcg atttctttgt
    18721 gattgccaag taaatccaaa aatgaaacgc cccgttcctc taaatcttca tgaccaatta
    18781 gtacacggat cgtattttca ttaatatgtt ccatttccat tttgctacac ctcactttgc
    18841 cacaatgttt aatatagatt cattgtagcg aaatcagctt aaatgtaaag aaaaaaaatc
    18901 tcgctgttaa gcgaatttaa taaaaccagc ggatttttca acctttcaag ctatcttatt
    18961 taactataac atttttggca gacgaatact atatagccga cttttcagct aattttccct
    19021 ctctattttt cgtgcaatca tcatcttcta ctaccttttt atacatctaa tcaagaatac
    19081 tttttgtaaa aacaaaaaaa cttggaaaaa ctatctttgt aatagt
//
LOCUS       DAYYCY010000038        19051 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000038 DAYYCY010000000
VERSION     DAYYCY010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19051
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.038"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(66..1634)
                     /locus_tag="ACHAZB_001091"
     CDS             complement(66..1634)
                     /locus_tag="ACHAZB_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HFM8613780.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(1638..1919)
                     /locus_tag="ACHAZB_001092"
     CDS             complement(1638..1919)
                     /locus_tag="ACHAZB_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFM8613781.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAAGAFSLYRVRMRKGA"
     gene            complement(1916..2839)
                     /locus_tag="ACHAZB_001093"
     CDS             complement(1916..2839)
                     /locus_tag="ACHAZB_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HFM8613782.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(2836..3714)
                     /locus_tag="ACHAZB_001094"
     CDS             complement(2836..3714)
                     /locus_tag="ACHAZB_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HFM8613783.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPTVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIETLLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(3701..5038)
                     /locus_tag="ACHAZB_001095"
     CDS             complement(3701..5038)
                     /locus_tag="ACHAZB_001095"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HFM8613784.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTSAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(5041..6144)
                     /locus_tag="ACHAZB_001096"
     CDS             complement(5041..6144)
                     /locus_tag="ACHAZB_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFM8613785.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(6218..7726)
                     /locus_tag="ACHAZB_001097"
     CDS             complement(6218..7726)
                     /locus_tag="ACHAZB_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM8613786.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(7692..8384)
                     /locus_tag="ACHAZB_001098"
     CDS             complement(7692..8384)
                     /locus_tag="ACHAZB_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFM8613787.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(8406..9380)
                     /locus_tag="ACHAZB_001099"
     CDS             complement(8406..9380)
                     /locus_tag="ACHAZB_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8613788.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            9680..10552
                     /locus_tag="ACHAZB_001100"
     CDS             9680..10552
                     /locus_tag="ACHAZB_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8613789.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(11134..13332)
                     /locus_tag="ACHAZB_001101"
     CDS             complement(11134..13332)
                     /locus_tag="ACHAZB_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8613790.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLFSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            13564..14556
                     /locus_tag="ACHAZB_001102"
     CDS             13564..14556
                     /locus_tag="ACHAZB_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8613791.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNLAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            14616..16037
                     /locus_tag="ACHAZB_001103"
     CDS             14616..16037
                     /locus_tag="ACHAZB_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFM8613792.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            16030..17529
                     /locus_tag="ACHAZB_001104"
     CDS             16030..17529
                     /locus_tag="ACHAZB_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HFM8613793.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            17568..18962
                     /gene="uxaC"
                     /locus_tag="ACHAZB_001105"
     CDS             17568..18962
                     /gene="uxaC"
                     /locus_tag="ACHAZB_001105"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFM8613794.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYLTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
BASE COUNT         5727 a         3882 c         3366 g         6076 t
ORIGIN      
        1 acaaacaaaa aaattcacta actagtgaaa ataagttagt gaacctcatt tattaaagaa
       61 ttaaattaca gatgatcttt tccatcatac ttgaaaaact caaaatcagc ataacttcct
      121 ctttttttat ttagatcatg cactgctatc ccgataaaat ttccagtaaa ccctcctgag
      181 agtatacaca tgtttttttg cggacctact ttcttccatt cttcagttga tacgtctttc
      241 aaatgaaact gtgctgtctc tttctttaca gtgactttca aaaaagtctt cttttctgtg
      301 agtggaatcg tccaatcttc tatttgtgct actccgtttt ctgattctat catacgtagg
      361 cattttccct tgtcctcatt ccaagtaaca taacaataaa tataattgga ttcatttaag
      421 tacagtacca gtccagccat ctgattaaaa ttattaggca tgtattcaaa tgctgtctcc
      481 gcatcaaaag taaaatccgt ttgccgaata gctaacaaat gatgttcgaa ggtagattga
      541 agagattcac cggaaatcag acgtaaaaaa cctggtctac attttgtatc acaccaatca
      601 gcatttggca aaatgcgtct ggcattccag ttcttcttta gctctccttc aaatgtatcc
      661 ataaactgat gatctttttc ttgttgtacc tcttcatttg tctcaatcat tatttcttcc
      721 atcggagttg tctggctatc tttaagtctt aaccaatcgt cggacgtcca atacacttct
      781 tgaatagcgg tttctcttcc caaaatagcc gcaccgttta atggtctggt acaaagatat
      841 gtcatgtacc atcttcctga cggggtttgt actaagctcc catgtcctgt acattgcaat
      901 gtagaagcag gtttgtcact agctgtcagc ataggaaaac caggatcaag ctcgtaagga
      961 ccagtaatat tctttgctcg gcaaactgta actgaatgtc ctcttcctgt accgccttct
     1021 gccgtcatca aataataata cccgttacga aaatacaaat gtggtgcctc cgttttcgct
     1081 aattctgtac ctgcaaatat tttatagatg ggaccatcaa gcttcttctt ctctgcatca
     1141 tattcttgta atacgatacc gctggattta ttcggtgtgt ccagacgata atcccatagt
     1201 tcattgacaa gccatttttt tcccgtttca agatcatgga acaaagaagg atcaaagccg
     1261 ttactattta aataaattgg ttcggaccaa ggacctgtga tctcagatgc agtgatcaaa
     1321 taattgtgga catctttaaa cggacggcct gcacttttga catctgtgta aacaagataa
     1381 aacaatccat cagcataact gatctgtggc gcccatatac ttccatttgt aggatttcct
     1441 tgcaaagata cttgagtggt caaaatatcc gttgtatgtg accagttgac taaatttttc
     1501 gactcatata ctcggattcc tggaagccat tcaaaagagg agacaataat gtaataccgc
     1561 tctttcactc ttaatatatt aggatctgga ttgaatcctc ttaaaatagg atttttcact
     1621 ttgatagtct gcatgattca ggctcctttt ctcattctta ctcgatataa actgaatgcc
     1681 ccagctgcta aaaaagcaag tgaaataaac aacacaacgg caaacagacc agtcatgtcc
     1741 tgtaataatc caaatgtatt ttctaaagac aaagttgaat gatctacagc aagcgactga
     1801 taactttttt taaactctga cgcagacata ttacttaaag taactgctaa cccgccaaag
     1861 aaaatcaaag atagactgcc accgaccatt cctagcaata gttcccattt tcttttcatt
     1921 tctgaacccc ctgcttttct aaatgtatcc attcaggtct tttgatcgat gtttcataat
     1981 tgttattacg ttcagagaat ttgacagtct gttcattttt cttgtcatcc caattatccc
     2041 aaccagttgg gtgaacatgc ccacctatct tacacgcttc aaataagaca tttgcatacg
     2101 gacgccacgg tcttcctaaa tagacgtttc tagtatcagg ttctgcagtc aaggtacagc
     2161 tttcaaaacg ataccctgtt ttttgatttt tcggcgtaga tgctgctgtg atgtatccag
     2221 gatttttact ggctaagcta tggatatgac actgatgaaa aacggcatct gccccaccaa
     2281 atatgaaatc caccgttcct tcaatccagc aatattcata ggtctgacga caatactcag
     2341 gattggcaga agccggtgta caaaaaggag taccatcttt ttgcaaatca ggcaacggac
     2401 ccgtgcataa ggtatcctga taaccagata gtcgacagtt ttttaaagat acgcgatctc
     2461 ctgaattgaa taaagcaaca gcctgtccta ccttttctcc tggacctgct gtattttcga
     2521 tgtgaagatt ttctaatacg acattcgatc cttctaagaa aaaagtagca cttcggaaag
     2581 tccctaactg gctaccgtca agatactcct gttttgcaaa acgattaccg gagataacta
     2641 ctgttcctgc tcccacaagt ttaaaattgg ataatctgct ttctatatat tcatcatatc
     2701 ttccttcttt tactatcaag acctttggat ggctatccgg caggcctgcc aaataatcga
     2761 ttccttcttg gatagtttca aaatccgtcg gggtatctat agcattcttt ccaattatca
     2821 gtttgtcttt catcctcata aatgctccct tatgaattgt ttccaaatat ttctcatttc
     2881 ttgtgtttcc atatgaccgc ctgttaaaga aaaagaagaa aagttttctg gactacccat
     2941 tgattgatac tttttgtcca gcccttttct cagcttcagt acgccttcgt ccaagcacat
     3001 cgtatcgttt tttccaacta agctaagacg agggcgcgga gcgatttttt cttggattgc
     3061 taacgttgta taagctgtca atagatttgg cacatagtaa taaaacccat gatgatctag
     3121 gcggcgatgt agcaaaagtg tttcgatgtc cacctgtgcc gctagatcaa tacaaacttt
     3181 gacacgagaa tccaatgctg tcacccacca gcttaataag cctcccatag aaagaccaat
     3241 cgttgcgagc cgttgagtat ctgtgtcttc ccttgtctcc aaataatcga tcaagctgat
     3301 cacatcataa atacgcattc cccataacgt ttttcctgtt aataaaaatt gtttgaacaa
     3361 ctcgctttca gaaatacctc cgcgttcttc aaatccccaa gcatcaatcg accaaacagc
     3421 atatcccatt cctgtcagta cttctgcaaa agatggagaa gctaagtaag gagcaccatg
     3481 gatcaattca cttttcccga ctgaaaaatc tccgccgtga gaatgcaggt aaaggaccgt
     3541 cggaaacggt ccttcttttt ctggataagc atagagggct ggtactgttt ccaattcatt
     3601 taagtacaat tgatagtatt caagcataaa accgttttgt ttttctcggt ataatcgttt
     3661 tccactaggg gtatgtctgc tagatggagc acctaaaaaa tcattcaact gcataagcaa
     3721 agtttttacg aacaaccagt tcatccatat tttctgattg atttgattgg aatttctcaa
     3781 tactgatttc tttagtattt tctaatagaa atgctggtcc ctcgtgattt tcaatcgaca
     3841 tctggttaaa ggaaacctta cccgcaaagc caatataaaa tccttgtctg ctcatttcct
     3901 ctattcctgt catcatagca ggaacaccgg gaactgcatc ttgggccatc gagacatcga
     3961 tattgtcaaa tgtgatttct tgtatgaatt gttccgctaa tccataaata aaacctgcag
     4021 aagcatggac attccttgcc gtgatattcg aaaaatggat ccttctgaat gctgatgtcc
     4081 gttcatctat cggataagcc tttttttccc atacatacgg ttctttccct cgcggaccac
     4141 agaaataata aagattcagt ataaatggac acatcacatt atccatgact atattggata
     4201 ctcgaatatc ttcaacgatc ccgcctcgac ctcgtctaga tttcaatcgt attcctcgat
     4261 cagtttcttg gaagatacaa tttgaaatgg taatattacg aatactacca ctcatttcac
     4321 ttcctaatac gacaccgcca tgtccatgga ccatcgtgca atttgtgatc gtgatatttt
     4381 cgcaagcgat cctttcgtac gtatcttcag tccctgcctt gattgcaata cagtcatctc
     4441 ccacgtcaat atggcaatta ctaatacgca catttttgca tgattctgga tcaattccgt
     4501 ctgtatttgg tgaatccgct gggttcaaga tcgtcaagtt atcaaacgtt gcattactgc
     4561 aaagaatcgg gttgatcgtc caacttggtg actggatcaa tttaatatct ttcacagtaa
     4621 ttcgatgaca gttatggaaa ctcatcaatt taggacgagg ataagcgaga ttatctggtt
     4681 catttctaaa agtatgccac catttttttc catttccatc taatgtaccg aaaccagtga
     4741 cagagatgtt ctctacattt tgagcataga tacaggaagc ataaactttt cgatggactc
     4801 cttcccaacg tgaatgaaca acaggataat cttttggatc gtcagagaat ttcagaaccg
     4861 ctcctgcgct taaatgaagt tcgacattgc tttttaaaaa taaagctcct gttagaaatt
     4921 cacctgctgg aacaactacg gtcccgcctc cgtcacttgc tgcagcatca atggcttgtt
     4981 gtattgcttc agtattcaat tcatcaattg atgcgccaaa tgtcaaaata tcatacatat
     5041 ttataatgcc tccacttcaa tcaatgcttg caagaatgca ccaacgcctt tttgatcatt
     5101 acaaataatt ggttcactga tgtagtaagc atatgaacca tctcgctgat ctgctccgcc
     5161 taaccctgct acttgacagt ttttattcaa gttcagccag ccttcttttg tttccagaac
     5221 aaattcatca agtaatcctt gatgtgattt cttcacttgt ggaagccatt cttctttgtt
     5281 cagcagtccc aaacgtattc ctttagccat tgcgtaaaca aacatactac tgccggaagc
     5341 ttccagataa tttcctttcc gttttgtttc tgttgttact tgataccatg tcccgctgtt
     5401 ttcttcttga tatggaagta atgcagccag tgtcttttct agaatttctt tcagcaaaga
     5461 acaatctgtt tcttcatgaa gtaattcata ggtatctgct gcagccatca aataccagcc
     5521 aatcgatcgg ctccaaaaat gatgggatag acctgttttt ggatcagccc atggttggac
     5581 cttcttttca tcccatgcat gaatcagcaa atttgttttt ggatcgaata gatgtttata
     5641 actgatttca aactgtctca aaacatcttg gtaatctttt tcttcactaa aattctggat
     5701 aaattcagca taaaaaggag aacccatata taagccatcc aaccaaattt gataaggata
     5761 aatttcttta tgccaaaaag ctccttcgga tgtgcgtgga tggtttgcca actgctggga
     5821 aaggagttct gctgcttttt tatatttttc ttcgcctgtt tctttgtaca ataaaaagaa
     5881 aagctttcct gtatttacat ggtcgatatt gtattcttca acgctgtagc cacggatcgt
     5941 tccatcttct tgcacgaaat agtcgatatt gtcttttatg aaatcaaaat aaattttttc
     6001 ttgggttcgt tcccaaagta attggaaccc tttaagtacg acgccatagt cataagacca
     6061 ttttccttta taccactttt cttcatataa gcgaggtgtt ctttccatga tactttttgc
     6121 taatttcttt gaccagtttt ccattatttg ctctccttta aaaattaaaa taggatttgg
     6181 gacaaatacc aagaagtatt tgtcccaatc gtctatatta ctgtgccgct gctttgtatg
     6241 cttcctcgta ctcttgtgtg attcgatcgc ctccttgtgc tttccagtct tctacagctt
     6301 ttttgaatcc atcttcgtca agctgtccca tgatgtattg caccgtagca tcaatgacga
     6361 ttttttcaat ttctgatcct acttcattgt tcgtcggtga atccaatggc acagaaggat
     6421 ccattacagc aaattcttca ttttctttga tcaattgatt agagaattct ttttcagggt
     6481 ctgtatcttt gaaagagaac gtcacttcac taggacgaga gcttgataac ggttgcacct
     6541 cttgttgcca aagatccata tcgataaatt cgatagaacc atcatcattc aattcataat
     6601 gcgttccttc gataccgcca gtcatcaatt tatacacatc ttcttctaaa agatcatcaa
     6661 caaattgaag taatttcttc aattgtgctt cgtcttttac ttgagatttg ggaaatgcta
     6721 gcaagccgcc aataccattt ccttctgacc aaacatgata accgccgcct tcaccatctg
     6781 agatcttgtt tacaggcact aattccatat catcttgaat accttgaccc atcgtcttca
     6841 gattcttgat atccaccatt ccagtgtaga ttccagttct tccttgtgca aattgttctt
     6901 gctgatcagt cttttgggta actgcaaagt cttgtgccaa atagccattc tcaaatagat
     6961 ctcttgaaaa attcaaagct ttcatatact cttctgtatc aaattctgga gtgaatttgc
     7021 cattatcgtc tacagcccag ccatttggtg ctccaaaata agaagtgaaa agtttaaagc
     7081 tggtataacg aatatcacta ggcgcacgat cgccaaaacc aactgtgtcg tccacaccat
     7141 ttccatcagg atcgttttct gtaaattgtt tagctacttc ataaagctca tccatggtag
     7201 ttggaacatc tagtcctaga ttatccaacc agtcttttcg aatgactaag ccagcacgag
     7261 cgtagtcttt ttgtatcggt acgccgtaca aggcaccgtc gatactcgca gcttcgattc
     7321 tatcttcaga aatttgactt aaatttttat agtcttttaa atattctgat acatcccaga
     7381 atacgcctga ttttaacgca ttgcggacag aagagttttt caacatcgca atttgaagtg
     7441 taacgatatc tcctagttca tcagaagcca atgcagttgt taatctttct tcttttgaag
     7501 catcaggaat ccagttgaat gtcagttccg tatcggtttt ttcttcgatc aagttcacaa
     7561 tcgtgtctgt aggcggtgaa gcatgatgca aaatatttaa ccaagtgatt ttagcttttc
     7621 catcttctgt ctctgaagaa ctgttacttg aactgctgcc gcatgcggca agtgctagaa
     7681 ttcctgcaaa actaagacct aattttacga gcgttgcttt tttcataaat tttctccttc
     7741 actgttttaa taatattttt gttttcttct gttttttctg tatatgagtt cttccattcc
     7801 aaatggcaaa atatagaatt tgctgtccac gtgactgcgg aaaagaagag actgccaccg
     7861 ataaaggtta aaacacctgg cattgtttgg atgatcatca gataaatccc taccatactt
     7921 aatatcaaca ctaatgagta atgaagatgt gagaatccga ataaaaatgc cattttcatt
     7981 ttgaaaaaat atcctttcca atcataatga gccatgattg ggaaaatata gagtgctgag
     8041 agcacatata tgaacgcagc aatatacaaa gcgatcctaa gataccaatt gtgtatgaaa
     8101 gaaagatcca caagtaaaac aatcccgatg gcgccatata tccagctcat cagcaaggtt
     8161 tcacgatagt tttctttaaa atatgtgaag tacgttttcg tcaaaggaaa cgtttctttt
     8221 gagcgcagcc attttctaat cactgcaacc atcgcatacg atgcaggacc gaaagtaaaa
     8281 actcccaaac ctgcgatgac taaaccgccc cacagaaaat tcaaataaac caactgtaaa
     8341 atacgattta gccttccgtt tattttatct aatgactgca gcatatcttt ccctctcttt
     8401 ctatcttagt aaacgccgtc ttctcctagc tttttggcaa tcttattagc taaaattacc
     8461 ataaacaatc ctacgacccc tttaaacaat cccattgctg tactgaaact taactgtcca
     8521 ttcttcaatc cagctgtata aatataggtg tcgaaaattt ctgcaacgct tcgattcaat
     8581 gaattcaata acaagtacat atgctcgaac cctaagtcta atgtctgacc gatttttaga
     8641 atcagcaagg taatgattac tggacgaatt gccggcaatg taacatgcca tgttttgcgt
     8701 aagcgtcctg ctccatccat ttctgcagct tcatacaact gtgtatcgac tgcagttatc
     8761 gctgaaaggt agatgatcgt agaccagccc aactctttcc agatcacttg tccgatatat
     8821 actgtgcgtg tccattcagg ggatgttaga aaactgattt tttcaaatcc cattgttgca
     8881 aggaattcat tcaatacccc tccgtcaaca ttcaaaaata cataagtaat cgaaacaatg
     8941 attacccaag acatgaagtg agggatgtaa atgatcgttt gaatcccgtt tttcaatcgt
     9001 ttgttcgtta cttcatttaa tagtaaagac aaaatgattg gcatcggaaa aaacacgaag
     9061 atatttaatc cgaacaagat caacgtattt cttaataatg taaagaatgt cggttctgta
     9121 aacaaacgaa tgaaatgttt gaaaccgacc caaggacttc ccattattcc taaaaatggt
     9181 tgataatctt gaaaagcaat aacgagtccc cccataggaa gatacctaaa aatcaagaaa
     9241 taaaggactc ccggcagaat catcacatat aaaaatttat tcgttttgat tcctgcaatt
     9301 attttttttc gtcttctgag cttttcttct tttttcgata gcgtcagttg aaatggttct
     9361 tgtttcttta cactttccat aagcccctcc ttagttttgt gaatgtttgc tgcttatgac
     9421 ttcatgatag ataactatct gaattacgta tataatcatt tttatcgtaa gtaaaaacgc
     9481 ttacatagca atgatttttg tgctatacaa aacgataaag tgtatttttt cacaaataaa
     9541 ctacgcatag caaaccttct atttttttct atctgacact tagcatagga aatgattata
     9601 tacgtaatct ttcaccgatg ataaattgaa catatagaaa cagaaacatt taatttattt
     9661 tgaggaggag cctatctata tgaaaaagaa agtctctata ggagaaagaa gttttacact
     9721 tttcaacaat acgtttctaa ttttgctagc tttgatctgt atcgttcctt ttttaaatat
     9781 catcgctaca tcttttgctt ctacacaaga agtcgtcgcg aaaaagttta tcctttttcc
     9841 tactaccttt tctttagacg cgtatcgcta cattctttct acgccgacca tttttagagc
     9901 acttgctgtt tcgattggtg ttacaggtgt cggaacgatc gtcagtatgt gtgcaacttc
     9961 actgatggcc tacggtcttt ccagaaagta tttgtttggt cgaggtttcg tcaactttct
    10021 tgttgtattt tctatgttgt tcagcggggg gatgattcca acgttcttag ttgttcgttc
    10081 tctagggctg gtcaattctt actgggccat gatcctgcct gtggctgtta atgcaatgaa
    10141 tatgatcatt atgagaaact ttttccaagc cttgccggat agtttggaag aatcagcaaa
    10201 aatggatggt tgcacagatt ttggtgtatt tttcaaaatt atgctgccat tagctttacc
    10261 gtctattgct actatctcgc ttttttatgc tgttacttac tggaacactt atatgactgc
    10321 tattttgtat atcaacgatt catctaaatg gccgatacag attcttttac gccaaatcgt
    10381 cattgtatca agtgggatgc aagcagaaag tagtgccgtt gatgtgattc caccagcaca
    10441 aacagttaaa atggctgtta tcgttattgc aacagtgcct atgctgattg tttatccttt
    10501 tgttcagaaa tattttgtca aaggagcact cgtaggttct gtcaaagggt gattatgtac
    10561 tgaacgcaaa atggtagttg tgtcagaagt ggtcagcttc aagaaataag aaggcattta
    10621 cgaaaattgc ttcttaaatt tttgtgaaat ttcggcttat ttttcgaaga atctactcct
    10681 ggaacaccgt gtatcagagg ttgtgaaaga gacgttcagc cttgagcgat gataaaaact
    10741 ctcacaaaca ttgcgacgag taacccacag gagcaataag aagcgatttt gaaaaatagc
    10801 ttcccatatt tcacaaaatt atgccttatt gtcgatagcc tgtctcttga acgccattta
    10861 ttcgtaaaaa agaactgtga caagactttt gtcacagttc tttttgcgtg cttagccttg
    10921 aaatttaact atccataaat acaaatacta gggttattct aacttctttt attgcttgaa
    10981 tcagtccaca agaaaaacag cctcttgatt aaacggcgtt tatcttgttg attctgcggc
    11041 aataaaatca gccagcagca aaacatgaag tgctgttttt tgctgtcctc ttttattgct
    11101 tgaatcagat cacaagaaaa acagcctctt gattcacaaa tatttttttc gatattctcc
    11161 cggcgtaatc gattccttct ttttaaagaa acgaataaaa ttttgcggat ttcgatattg
    11221 caatctttct gcaatctctt ttacggagag gttagtctct ttcaaccatt tttttgctac
    11281 ctctaaacga tgattttgaa caaaatcacc aaatttttct cctgtttcct ttttaaatac
    11341 attacttaaa tagttagggt tataatgcaa cctatcagcg atcacatcta aagacagatc
    11401 acgatcatat tctgtgtaga cgatatgcat aatattctcc gacaaatttt taaattcatg
    11461 atcagtgatt gcttgagagc attctgtgat tggcagaact aattgttcta aaacaagttt
    11521 ttctagttct ttgggatgat aattgttcag tgcttttaca taaatttgtt tcatgttatc
    11581 aaagtttttt gaatctactc ccaataactg tcctaattga acgagttcat tgatcaagcg
    11641 gaccgaaact acttcccgac tcaaaggatt tttattttgt gcaaacaatg catcaatcaa
    11701 ttcatgtact aaattcttca cttcctgatt tccagaacga atggcttcaa acaatcgatt
    11761 ttgttcttct atcggatatc taataagtgt tttttcatga gcagcaggaa cgatttcctg
    11821 ataaaaaata attgaacttg gaccagtatt tacacgataa tacagggctt cttttgcccg
    11881 atcaaccgat tgcttgcttt cattcagcgt ttcgaaccgg ttactgatgc ctatactgat
    11941 agttaaattc aagtattctt ttacacgttt ttgaatgagt cggcaatact ccatgacttt
    12001 tttgcttgct tctgcatcat ctttatccat tcgataaata gtggcttgca tttcttcatt
    12061 caaaacgatc ggcagcattc gattttcctt tggcacaatt tccgtgatca tgttgttgat
    12121 cgctaaaagt agcaaatcac gctccccacc gtgactatct tctaacagat cgatttgaac
    12181 caaagctgta taatagcagt cattttgaga actataacca aattgctgca accgctgatc
    12241 tagttctcgc ctttctactc gatttctaaa taaactaagt acaaatagcg tttccagctg
    12301 tggcttttgg gtaactaagt gtgcacttaa cgcttctttt tctcctatga tccgactgac
    12361 tgattgagcg accaaatcta gttctttccc actgaatcct tttgatttaa gattcagacg
    12421 ttcctgtatt tgagaaatcg gacgggcaaa tctgtccgaa aataagtagg aaaaaagacc
    12481 aatgagtcct accagtgtta aagacactgc aactagcccg attctcagat tgcggatcat
    12541 tgcgccaatt gcactagagt caacttctaa tctgtaaatc caattattat aatctgattt
    12601 cagtactata tacgttttat ctttttctct tatcactttc agctgattgt aatctagatc
    12661 cacgtccgtt aatacagttg gttgctcttt attttttact gtctgagtag taaaaaggtt
    12721 gccaacttcg ttttcaatag aaagtatgtc ttctccactt tcaccaacaa cttgttctat
    12781 tgtatgtttc tctatcgtag caattcccaa tgcatatttt tctttttcaa acattggcaa
    12841 agaaatcacc atttcgattc ctttgttttt cttttgccaa aataaattat ttttgttatt
    12901 tatgtacttc tgttgatact tttcaacttc ctcttcggtc agttgactca acgatccatt
    12961 gattactccc catttaccag ccaaactgac taaatcataa ttattatcat ccataacaat
    13021 cgtttctata aaatttagtt ctttcctgat ttcagaataa gtggagtaat ctgtatatgt
    13081 cagcggatgc ttgaatatgt ccgagaaatt tttggtcgaa ccatacgtag aaaacgaata
    13141 ttcaatcgtt ctaattttcc attcaacgtt agttttaact tgcctgagca aattcttttt
    13201 gtctgttaaa taactcgatt cgatcgattc ataggtattc tggtaaatga ataaactaaa
    13261 tcctatgaca agtacggctc ctaataaaaa catcggtaaa aatattttat aaaatactgt
    13321 acttttcttc attttcttcc tctcctcctt ttaaaataaa aggctttcat ttttctgtaa
    13381 aattgatact catctaatta aatgagcatt ccccgctttc aagaatagca taattaaaaa
    13441 taaaaatcca agtaattttt aacgtgtgca atcttttcat atcaaagatc tattgactta
    13501 tcgtagttat cgtaatatga tgaatgcgtt aacaaaaagt gaaaaagtaa ggaaaatgat
    13561 ggaatggtaa cgatcaaaga tatcgctaaa gcagctggcg tttctcatac gacagtttct
    13621 cgtgctttga ataacagcag tttaataaaa aatgaaacaa aacaaaaaat acgacaacta
    13681 gctaaggaaa tgaattattc gccaaactat agtgcaaaag gtttagtgaa tcaaaaaaaa
    13741 tatctaatcg gactattttt ctcaagtttg cataaaggaa cttcatcaaa tttcctagca
    13801 gattccattg caggcatcca ttcagtatta gacacaaatt acagtttatc tgttgaaagt
    13861 atcgatgttg ttgatttaac agaaatcaac ctgcaacgct acgatggact gatcgttatg
    13921 agtcaaagtg atgatgatca gcctttcatc gactatctca aagaaaaaga atttcctttc
    13981 gttgttgtca atcgtcacat caatgatcca gcggttatca acgtcactgc cgatgatgcg
    14041 gccggtgtta tgcaggcagt agattacggt attggatgcg gacatacgaa gatcgcttat
    14101 attggcggaa agaaaaattt tcgttcgaca aaagaacggg aaaaaggggt attgaaaagt
    14161 ctaaaaaaag cgagcatccc tgtcaatcta gcctattttt taacaggaga ttacagctta
    14221 gaaagcggcc tccaaaacat ggaataccta ttatcacttt ctgacctgcc tactctggtt
    14281 ttttgcggca acgacgacat agcgattggt gcattgagag ctgtacattt agctggcctc
    14341 cgtgttcccc agaacatttc tctgatcggg ttcgatgatt ctccagtcgt ctcctatctc
    14401 aatccgccgc ttaccaccgt acgtaagcct ttgaaagaaa tgtgtcaaga aggaacaaag
    14461 cttttactag atttgatcga aggcaggacg atcgaacaat ctaaaattga gctctccacc
    14521 ttgttgatcc ttcgtgagtc gatcaattac ttgtaaacta caagtaattt tatataaaaa
    14581 tgttaacgtg tgcataacac agaaaggaag tttatatgaa aaaactgaca aaagaattag
    14641 tgaaaaaaaa cgaagcacct gtaaaagttc ttcagtttgg tgaaggcaat tttatgcgtg
    14701 gatttattga ttggcagatt cagcagctga ataaccaaca attgtttaaa ggtaatgttg
    14761 caatcattca acctttagaa caaggtcttg gaaatatgat gaaccagcaa gataatttat
    14821 atacggtcat tttacaagga ctcagcaatg gcgaaatcgt tgatacttcc gagattatca
    14881 ccgtagtaga acaaatgatc aatccttatg atgaatggga tgcttatcta tctctagcag
    14941 aaaatgacga cttggcctat atcgtctcca atacaacaga ggcaggaatc caattcgtgg
    15001 agactgataa tttaacagat actcccccac agagcttccc gggcaaactg acagcctttt
    15061 tataccgacg tttcaaatta aacaaagctg gctttaccat cattccttgc gaactgattg
    15121 acagaaacgg cgaacgtcta aaagaaatca ttttacgcta cgcagaaaaa tgggatttag
    15181 aacctgcttt tgttgagtgg ttagaaaaag aaaatgtttt ctgttgtagt ttagttgatc
    15241 gcatcgtccc aggttatcct cgcgaagatg caggtgtttt caatcaacgg cataactatg
    15301 aagatcaatt gatggtcaaa gcagaaccat tcatgctttg ggtgatcgaa gggcctaaag
    15361 aattggagaa tcaactacca ttcaaacagg ctggcttgaa tgtcatcatc acagatgata
    15421 tgactcctta tcgtgaacgt aaggtgcatt tgcttaatgg accgcataca gcgatggtcc
    15481 ctcttgcttt gttggcggaa ttggaaactg tagaagaagt aatgaaagat cctttattct
    15541 ccgcatatgt tgatcagtta ttcaatctag aattgattcc tatgctttct ttacctaaag
    15601 atgaattagc catttatgct gaccaaatca aagaacgttt cttgaatcct tttgcccatc
    15661 acaaattaga agcgatttca ttaaatagtg tttcaaagtt cagcacccgt ctgcttccag
    15721 tgttcaagaa gtatatcgaa gaacaaaatc aggtaccacc gttaatcact gtttcactag
    15781 ctgcgctact tcttatgtat cgaggcgatc aagtaaagcc acatgatgat gaaaagacga
    15841 taagcgaatt tactgatgct tggtctgata atggaacagc tattcctcga ttattgcaaa
    15901 acgctgcgct ttggggagaa gatttgtctc aaataccgaa tgtgacagat acagttcaag
    15961 aaatagctgc aaagatcgat caaaaaggtg tccggtcagt attacaagaa atgaaaggaa
    16021 cgatggtcca tgcttgaaac cctgcaacca acaatgaaat taaatcgacg agacagtgtg
    16081 gtcgtcgcat taacccctat tccaaaacaa acacaactga cgattgatca gcaaacgatc
    16141 accactcttg aagatatccc gcaaggtcat aagattgcat tgggtgattt gaaagctgga
    16201 gacaacgtga tcaaatatgg ctatccaatc ggtcatgtga cgacagacgt aactgccggt
    16261 caatggctgc acactcataa tgtaaaaacc aatctttctg gtgaattgga ttatcgatat
    16321 gaaaaagatg tccatccaag tcattacctt tttgagaacc gaacatttca aggttacttg
    16381 cgaaaaaatg gaaaagtagg gattcgtaat gatttgtttt tagtacctac cgttgggtgt
    16441 gtgaatggca ttgctgaact gatcgtcaaa cagttcaagg aaaaacatcc agatctggga
    16501 cagtttgacc atattacgat tttaaagcat ccttatggct gttcgcagct tggaaaagat
    16561 catcaaaata ctcgagaaat tttagcggac gcagtaaatc atcccaatgc cggaggagtt
    16621 ttagtatttg gattaggttg tgagaacaat acagttcctg aattcaaaaa aattttaggt
    16681 gaatatgatg aagaacgagt gaaatttcta gtggctcaag atgtttacga tgagatcgaa
    16741 caaggtgtgg ctctattaga agagctttta acagcggcgg aaaatgacca gcgcatatca
    16801 gtcccgctct ctaagttgaa tgtcggtttg aaatgcggcg gttctgatgg tctatcggga
    16861 ataactgcta atcctttact tggtgcattt tctgactact tgattgctca aggaggcagc
    16921 acgatcttaa cagaagtgcc agaaatgttt ggcgcagaac aagtattgat ggcacgagcg
    16981 gaaaatgaag aagtttttga ctcaatcgtt gacttgatca atgacttcaa acagtatttc
    17041 ttgtcttacg gggaaccagt ttacgaaaac ccatctcctg gtaataaagc aggtgggatc
    17101 actacattag aagataagtc tttaggctgc acgcaaaaat caggaagctc tcctgtcgtt
    17161 gatgtgctac aatatggcga aaaaatcaga aaaccagggc ttagtttatt gcaagctccc
    17221 gggaatgatc ttgtagctgc ctctgctctt gcttcatccg attgccagtt ggtcttgttt
    17281 accacaggaa gagggactcc gtttggctca tatgtcccaa cggtcaaggt atctacgaat
    17341 acaactcttt ttgaacgcaa aggtcattgg atggatttta atgcaggcgt cttattagaa
    17401 caaccaatgg aagtaatctt agaatcgttt gttcaaaaaa tcatcgctgt agctagtgga
    17461 gaagaaacga aaaatgaaca aaatgacgtt cgagaaattg ccatttttaa aaacggagta
    17521 acactttgat tttcataaaa cagtataaaa agaaagaagg aagatttatg tttttatctg
    17581 atgatttttt attaaaagac gattgggcga aaaagcttta ccattcctac gcaaaaaaca
    17641 tgcccattat tgattatcac tgtcacttag atccaaaaga aatttacgaa aacaacaatt
    17701 tcagcaatct gacagaagct tggttaggag gcgatcatta caaatggcgc ttgatgcgag
    17761 cttgcggcgt gcctgaagaa aaaatcacag gaaatgcttc tgattttgaa aaatttttag
    17821 cttggtgtca aaccgtacca aaaattatcg gaaatccgct ttacagttgg tcacatcttg
    17881 aattgaagcg tttcttcggt attgacttgt tgataaatga agaaaatgca gagaaaattt
    17941 ggacacaagc aaatgaaatt ttggcagctc ctgattttcg ccgtcgggaa atcgcaaaaa
    18001 aatcgaatgt agaagtgatt tgtacaacag atgatcccgc agacgacttg cactatcatc
    18061 agttgcttgc tcatgaagaa ccagatttga aggttctacc ttcattccgt ccagataaag
    18121 cactcaatat tgaaaaagaa ggatttaatg catgggtcac actactggaa gaagcagctg
    18181 accaaacgat caccacctat caagaactga tagaagtctt agggcagcgg atagaatact
    18241 tccatcaaca tggatgtcgt gtttccgacc acgctttaga catcttacgc tataaagaag
    18301 cggatgaaac agtattagaa gagatttttc aaaaagcacg agctaacaaa ctattatctt
    18361 cagatgaaat cgatgcttat cttacagaaa cactgataca tttgacccac ttctatcatt
    18421 ctcataattg gacgatgcag ctgcacatcc atgcttatcg taactgtaat acacaaatgt
    18481 tggaaaaatt agggcccgat accggctatg atggaatgaa tgatctttct ttgactattc
    18541 ctctgcaaaa attattgaac cgtgcagaag aaacggatca gttgccaaag acgatccttt
    18601 attcattgaa tccgaatgac tatcctgcga ttttagcttt gatgggctgt ttccaaaaag
    18661 aacagaatgg aaaactgcag ttaggttctg gatggtggta caacgatact cgagctggta
    18721 tgcgcgatca attgacacaa tttgctgatg gtagtgcttt ggggaatttt gtcggcatgc
    18781 tgacagattc aagaagtttc ctgtcctaca ctagacatga atatttccgt cgtgttcttt
    18841 gtgaattttt aggcgaaatg gtcatgcgag gagaagcacc tgaagatgaa caacttttag
    18901 gaagtctagt tcaagcgatt agttatacaa atgctaaaaa ttactttggc ttttttgcct
    18961 aattgattaa tagtaacagt aacgaatcaa aaacaggata gtgaaataac ggaatccgtt
    19021 tatccacgtt ttagagaaga agacggacca a
//
LOCUS       DAYYCY010000039        18621 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000039 DAYYCY010000000
VERSION     DAYYCY010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18621
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.039"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            137..844
                     /locus_tag="ACHAZB_001106"
     CDS             137..844
                     /locus_tag="ACHAZB_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFM8613795.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            831..1154
                     /locus_tag="ACHAZB_001107"
     CDS             831..1154
                     /locus_tag="ACHAZB_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFM8613796.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(1243..2280)
                     /locus_tag="ACHAZB_001108"
     CDS             complement(1243..2280)
                     /locus_tag="ACHAZB_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HFM8613797.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(2601..3584)
                     /locus_tag="ACHAZB_001109"
     CDS             complement(2601..3584)
                     /locus_tag="ACHAZB_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HFM8613798.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(3999..4769)
                     /locus_tag="ACHAZB_001110"
     CDS             complement(3999..4769)
                     /locus_tag="ACHAZB_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8613799.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(4762..5697)
                     /locus_tag="ACHAZB_001111"
     CDS             complement(4762..5697)
                     /locus_tag="ACHAZB_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8613800.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(6013..7662)
                     /locus_tag="ACHAZB_001112"
     CDS             complement(6013..7662)
                     /locus_tag="ACHAZB_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFM8613801.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(7779..10382)
                     /locus_tag="ACHAZB_001113"
     CDS             complement(7779..10382)
                     /locus_tag="ACHAZB_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFM8613802.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(10394..11443)
                     /locus_tag="ACHAZB_001114"
     CDS             complement(10394..11443)
                     /locus_tag="ACHAZB_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFM8613803.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(11469..11783)
                     /locus_tag="ACHAZB_001115"
     CDS             complement(11469..11783)
                     /locus_tag="ACHAZB_001115"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFM8613804.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(11799..12245)
                     /locus_tag="ACHAZB_001116"
     CDS             complement(11799..12245)
                     /locus_tag="ACHAZB_001116"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFM8613805.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(12246..14168)
                     /locus_tag="ACHAZB_001117"
     CDS             complement(12246..14168)
                     /locus_tag="ACHAZB_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM8613806.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEFYPDEELINNLVL
                     HLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEEIEERFCIHFNEDEIAYVT
                     LHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVLSINELQDRK
                     VTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFLNLLDKRFCF
                     FTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKLSSTAMNDFAMPHIEVDFI
                     KKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKIYETFNEIASNHELLANLK
                     TSSSFEKLVYILKEGL"
     gene            complement(14380..15276)
                     /locus_tag="ACHAZB_001118"
     CDS             complement(14380..15276)
                     /locus_tag="ACHAZB_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM8613807.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(15269..15949)
                     /locus_tag="ACHAZB_001119"
     CDS             complement(15269..15949)
                     /locus_tag="ACHAZB_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8613808.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(15950..16732)
                     /locus_tag="ACHAZB_001120"
     CDS             complement(15950..16732)
                     /locus_tag="ACHAZB_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFM8613809.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(16725..17642)
                     /locus_tag="ACHAZB_001121"
     CDS             complement(16725..17642)
                     /locus_tag="ACHAZB_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFM8613810.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(17806..18060)
                     /locus_tag="ACHAZB_001122"
     CDS             complement(17806..18060)
                     /locus_tag="ACHAZB_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8613811.1"
                     /translation="MSTITTITMVASPLGLALFGALFDWKTTQPRLRDSLIFLGGSLA
                     IVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEKKESGN"
BASE COUNT         5424 a         3910 c         3091 g         6196 t
ORIGIN      
        1 aagatttttt taaactacgt ccagtctttt ttatttgttt aaaattgaag ttgacaagtc
       61 ttcattaaaa atttagaata ttcacaagtt tgacgacaat tatgaaaatt tatgtcgtgc
      121 taaaggagtt ttgttattgg atgaaaagtt agatataaaa actgcgatca aagacacgtt
      181 acctaccgtt tttggttata tcggtattgg acttgcattt ggtatcgttg ggaaagctgc
      241 cgggtttcat ccgctggtcg tcacgttgat gtccctgctg atctatgctg gttctgccca
      301 atttatcacg gtcagcatgc ttgctagcca tagtccactg ctctccatcg ttctctcgac
      361 ctttctagtc aattcccgaa tgattctgat gagtatgacg attgctcctt atttcaagaa
      421 aaatcggctg cttcaaaatc tgctgattgg cacattgctg acagatgaaa gtttcgcact
      481 gggaatgaat aaattgaact atactggaca aaagttaaac tttcgttgga tgaatacagc
      541 taattggatt tcttatttga cgtgggtggg ttcttctctt gttggcgcac ttttagggaa
      601 ttttattact gatccgaaaa agtttggttt ggatttcgcc atcgtcgcta tgtttatcgg
      661 gttactttat cttcaagtca tttctgatag aaacacctct aaacgattac agttgatcct
      721 gattggtctc actttgattc tggtatatgt cggactgatt tttatcccaa gtaacttagt
      781 gattttagtt gtaacactga ttgggtgcgg attgggggtg tggatcaaac atgccttctt
      841 ttgagtttac attgctgact attttaggtt gtactttagc aacttggctt tctaggatcc
      901 taccatttgt cttactgaaa aaattcgatt tgccgcaacc gttgcttgag tatttaagtt
      961 ttgtcccgat cgtcattatg tctgctttat ggttcagcag tctatttacc caaaatatcg
     1021 gacatctgcc gcagatcaat ctcgaaaatg ctttggcttc agttcctaca cttttagctg
     1081 ctattctttc aaaaagctta ttggtgatcg tcttggtagg aattctttcc ctttcgttga
     1141 ttcgcttatt ataaataaaa aaacaagagc ttatggcaaa ttactgtcat gcgctcttgt
     1201 ttttatcaaa gtcaaataag aaaaaaatcg gcgtattctt gcttatccgt tgaacggacc
     1261 actaacaacg acttttccta atatatgtcc tgtttctacg tctttggttg cttgtttcaa
     1321 gttcttcgca ttcatcccat ctgaatagac cttgctcaat gtactgcgga taatcccttc
     1381 atttgctaaa gcagcaatgt gcgctaaagc ttctccttgc gtttcgattt catagttata
     1441 gtctgtttta gcgaacatat actcccagtc gaagctgata gattggtttt tccaaacacc
     1501 tatatctaac ggctctttga tccctacgat cgttcccaca tgtccaaatg gtcggacaag
     1561 gtgtttgatt tgatcaaagt agcgggtaat atcgaataat acagcgacat actctacttg
     1621 atcgattcct aattcacgaa aacttttact taagtcgtga tgataatcga ttggatagtc
     1681 tacacctgtt tttttcagcc attcaaaatt cttcggactt gcagtcgcgt agacttctag
     1741 gccagcccaa tgcgctagtt gattcaaaat cgaaccgacg cccccagctc cgttgatcac
     1801 taagattttt ttcccgcggt tggcattttc ttctggagtc agaccaaatt tttcaaataa
     1861 caattcataa gcggtcaatg aggtcaacgg caaggctgcg gcttcttcat ctgaaagatt
     1921 ttttggtgca agagcgacaa tacgttcgtc aaccagttgg tattcttgat tgcttcctgc
     1981 tcttgtcgtt gtacctgcgt aaaaaacgcg atcgccgaca gaaaattttt gcacttcatc
     2041 acctacagca acaactttcc cgactccatc aaaccctaag atacgtaaag catttcttat
     2101 cccgttatct tggcgaagtt tcgtatcgac aggattaaca gatactgcgt tgacttttac
     2161 cagaagatcc cgtgaactgg gtacaggtat ctgttctttt tcatcaataa agctttttgg
     2221 atcatcgatc gacaaccctt cataaaaacc gactaccttc attaattcct tttctgacat
     2281 acgaattcct ccttagcttc atcaagttgg agcatccata cagttttcat ttcaatatat
     2341 atagtatgct atttgttcca cttttattca tcataattat tctcttactt aaagtaaact
     2401 atttgcactt ttttggttaa aaaagaggtt gcgacaagac ttttgtcaca acctctttta
     2461 aacggtgttc aatcaactga ccctcggtat tcgctcaaaa aacgacaaaa tatgaggagc
     2521 tatttttgtt tggctgttct tattacttga tgcaggacag ctttttcacg acctctaatt
     2581 cacggtgttc aagcaagaaa tcattgttca ctgaaaagcg ttggatgttc tttttcaaag
     2641 gtgtctaaga tctctcgtac ttcatcagta gatttcgtat tcatcaactg aactcgcaag
     2701 tcatttgctc ctggaaatcc tttgatatag attttaaaga aacgatgtag tcctgtgatg
     2761 gaacgcggga tgatttctga ataatgatct tgcagatcta gttgcagacg aagtagtccg
     2821 atcagttctt tttctgtatg ttccctcggt tctttttcaa atgcataagg attcttgaaa
     2881 atcccccggc caatcatgat gccatctacg ccatattttt cggccaattc ttctcctgtc
     2941 tggcgatctg ggatatctcc gttgatcgtg atcagtgttt gcggagcgat ttggtcacgt
     3001 aaagcaatga ttttagggat gacttcccaa tgtgcatcta ctttactcat ctctttacgc
     3061 gtacgcaaat ggacagaaag attggctata tcttgatgaa gtaggtgact gatccattct
     3121 tccatctcag acatttccgt atagccgata cgcgtcttga cgctgacagg caaaccgcca
     3181 gctttagccg cttcaatcaa ttgtgccgct acttctgggc gaaggatcaa gccactgcct
     3241 tttccacgtt ctgctacatt agggactggg cagcccatgt tgatatcgat tccttggaag
     3301 cccatttctg ccatagcaat gctcatttct ctaaaaaatt ctggcttgtc tccccaaata
     3361 tgggcaacca tcggctgttc atcttctgta aaggtcaatc ttcctcgtac actgtctttt
     3421 ccatctggat gacagaaact gtctgagttt gtaaactcag taaagaagac atccggtgct
     3481 gctgcatgct ttacgacatg gcgaaataca acatctgtta catcttccat tggtgctaaa
     3541 acaaaaaaag gctttggcag ctctttccaa aaattagtag tcatttattt gttactctcc
     3601 attctatcaa gcagtgaagt cgcaatcttt ctcaaaaaca ttgtcattat actaggaaac
     3661 aaaaaaagaa gcaaagtgaa cagcttaaaa gatgtttctg attgagcaaa aaaagactgt
     3721 gacaaaaatc ttatcacaat tccttttccg gaataaatgg tgttcaaaag cttgcatctg
     3781 cggtaataag tccaaccaaa cgaaaaatag gaggagctat ttttgtttgg ctgttcttat
     3841 tacttgatgc agaacagctt tttcacaacc tctccactaa acggtgttca atcagctgac
     3901 cctcggtatt agctcaacaa acgacaaaat atgaggaact attttcccgt ttgtttctct
     3961 aatactcagg tcaaaacgct gatttcacaa cctcttttct aacttctcaa tcctcttcct
     4021 ttattgatca aataccaagt aacggcgtac aaaacaacga tgaatagcac aagtacgatc
     4081 atcgatgttg ctactggcac atcgattgtt ccaaggaatc cgtaacggaa accggagatc
     4141 atataaacga tcggattgac tttcgaaaca gcttgccaaa atggcggcaa catagagatg
     4201 gcatagaaaa cgccacctag ataagttaac ggctgcaaga caaatgtagg cacgatcgat
     4261 acatcgtcat aagactgagc aaaaatcccg ttgagcagtc ctgctaacga aaacaagatt
     4321 gccgtcatca ataatgtaat gatgaccatg aaccatgaat acacatgaag tggtacaaag
     4381 aacaaggaaa tgatcgttac gagagagccg acgagaatac tgcgacccac tccgccaatc
     4441 acaaagcccc aaatgatcac atgcgtagga acaggcgcta ccaagatttc ttcgatgttt
     4501 ttctggaatt tttgagagaa gaaggacgaa gaaacgttgg catacgaact agtgatagca
     4561 gacatcataa tcaaaccagg cacgatgaat tccatatatg aaaaaccacc catgtcaccg
     4621 atccgaccac cgatcatttt tccgaaaata acaaagtaca aggatgtcgt gatcacaggc
     4681 ggaaccagtg tttggaccca gatacgcaaa tagcggttcg tttccttgat agctaaactt
     4741 tttaatgcag taaaataaag attaaacatt tttctcctcc gtatgattat tttcttctgt
     4801 gattttcaaa aacagttctt ctaaacgatt cgacttattc ctcatagaaa gaactttgat
     4861 tccttgtctt gtcagctgat cgaagattcc gttcacccct tgatttcgtt caacttcgac
     4921 agcgattgtc tgttcatctt caaatgtaca agcgtatcct tcaatcacag gtttggtctc
     4981 atatggcgct aaatcaaaga taaatgtttc aaattgcagt ttggatagaa gctctttcat
     5041 actagtattc tcgatcagtt cacctgattg gatgatcccg atgttgcgac aaagcatttc
     5101 agcttcttct aaataatgcg tcgtcaaaat gatcgttgta ccattttcgt tcaattctcg
     5161 gagaaactcc cacatctcgc gtctcaattc aatatcgact cctgctgttg gttcatccaa
     5221 gatcagcaaa cgtggttcat gcatcaatgc cctagcaatc atcagtcttc gcttcattcc
     5281 cccggaaagc atacgtgcac ggacatttct cttttcccaa agattggact gcttcaaata
     5341 tttttcgctg cgtttcatcg cttctttgcg agagacaccg tagtagcctg cttgattcac
     5401 gacgatttgc tggaccgttt caaagggatt gaaattgaac tcttgcggga ctaatccaat
     5461 ctgttgcttt gctcggacta gatccgtatc cagatcataa ccgaagacat tcacttttcc
     5521 agatgtttta ttgactaaag aagtaatgat tccgatcgtt gtagacttcc cagctccatt
     5581 tggtcctaat agcgcataga aatctccttc tttcacggaa agatcgattc ctcgcaatgc
     5641 ttcgacccca cttccataaa cctttttcaa attatttatt tcaagcgcat ttgccattta
     5701 tttcctatct ccttatagac atgattcttt attgtatcat ggaatacagc agatgaaaaa
     5761 acaacttggt tccagcgaaa aatcaggttg ctgggctcag gaaaatctcc taatgaaaga
     5821 catcaatcgg acgaagtcag acaacctttt cagaaaaaac aaatcgaaga taaaggatta
     5881 ctattcatat gtgcaacaaa aggagcgtga gacaaaagta aagaacactt ttgtctcacg
     5941 ctctaaacac gtataaacgg cggaagcaaa agccaagatt tttgatccaa taaatacttt
     6001 cgatcacttc gcctatccca atacatgcca ctgccgcttt tcatcaaata acgcggcttc
     6061 cgtttttgcg gtttctacta aagtctctgg gtaatccaaa actttcagca aggcaatcgc
     6121 atttcgggtc cttgaaggtc cttcttttaa tttgaaatca aaactgattc cttttccttc
     6181 tgttacttgt tcttcaaagt ggacattctc acaataattc tttaaaatct ctgtcaattc
     6241 gatatcatgt gtggcgacaa atgctaaaga tggatactcc gcaagccacc gaacgactga
     6301 agcggaagaa gcaatccgtt cgatcgtatt tgtccctttt aaaatctcat ctacgaagca
     6361 gtagcagcgt tctttggaag ccacttggtc caacaaacgt ttgatcgatt tgatttctga
     6421 aacaaaataa ctgtcgcctt caaataaatc atcttcgatt gccatggaag tcaacacatg
     6481 tccaggttgc agggtaaact gttctgcaat cgctgtttgg atcgtctgtg ctagaataca
     6541 gctgatggct atacttttga catacgtaga ttttcctgat gcattggaac ccgtgactaa
     6601 tgtattcttc tgccagttga ccggattcac taccgcttct tttaacaatg gatgatagat
     6661 atctttcgct gtcacaccgc cctcagtaaa ctctggtcgg caagtgatgg gcatataagt
     6721 acggtaattc aatattgcag cggctacttc taattctccc agcaattccc acagcttgat
     6781 ggcttcagtt gaatgctttt ctaatttagt gagaacgaaa tgataggaga taaatggcag
     6841 catgaacatg atgttgacat attcaaaaat caactctgct tcactcccac tcttcatacg
     6901 aaatgaaaaa cctaatttag ggatgtctct taatggttta gtttgctgtt tcagctcttc
     6961 ttgaagtggt gtcggcagtt ttgctagctg attggcggct gaaatcgttg aaaccaaata
     7021 actcatgcta ttcagctctg tttctagtgt ttgtttcttc actagataat agaccgtatt
     7081 gaatactaac ccaccgattg ccaaatagat tcctagtact tgtcctaaaa gaagcaaacc
     7141 caaacccacc agcggaagca agccaagaaa aataaataaa cctacgcgtc caatcttttt
     7201 actttttcca tccgctagat attgttttgc aaaattatga tctttttttc ctaaacgagc
     7261 gaattgatat tggatttttt ctctcagttg aggattttcc tgataaaaag caatcagttt
     7321 ttctaactgc tgatcttcac caaaatcaaa gctgcgtaaa cgttgataca acgcttctga
     7381 acctacacta gaataggtac tgttgattcc ttcgaatact tcgaacatat ccaggtcata
     7441 ccaagtaaga tcatcgattt cgctatccca tgaacggaac ttcttctccg tgagccatgc
     7501 ttccttcaag ctttcttctt tatcaaatct cggttgataa ggaatttttt cccatttgct
     7561 acgaactgtt tcctttaatt tcaaacgtga aaaaatgtct aaagcaacta ataaaataac
     7621 agctgcaata ataattaaaa cgataaattc tctttgaccc atatccctct ccgttccttt
     7681 atttgttagc tttagtatag cactgttgcg ctatttgaaa agagggtaaa aaaacacctt
     7741 tcaaaataaa aggtgttttt ttcttgtata ttacatattt atctctcatt tatcaaaatt
     7801 gacaaataat catagggagg aattgttgtt aattcttgtt tattctccag tgcattgaca
     7861 actgtcccaa gggaaaggat agcctcaggt actatttttt cttgttctgt tggattagct
     7921 aagcgaagaa caaatccttg ttcatgaaaa taagaaggat aaatcgatga tacaattaag
     7981 tcttcaggta attcaatcaa tgaaaattta cgaggaacat caagttcatt ttgtaatggc
     8041 caaatcttgt tatctaacct gtgaataaat acattcagtt tctgcttttg ataagaaata
     8101 gattgttcta accgtttttc tgcctgtttt gccactttga actcattaaa tcttccttct
     8161 tctacattaa atccaaaagt gactttatac tcacctatct cttgagctag cggtgtttcc
     8221 atcatgacat gcccttcatt tgtcgtatct cctgaagctc gcccaggtcg ccaagcaaga
     8281 ttaggttttc caagttcccc tgttgttgaa aataaggtta tgattaattg attttctata
     8341 cgttcatact cttttaaacc atctgcataa aacgtaaaat aatgatcatt ttctgccaca
     8401 gagacactct tatcgaatat ttctaaatta acaggtttct ctaccatagt ttctgaccaa
     8461 ttcggaggaa tgacatcagg ggtattttca ataaatccgt tttgaatttg tgcaattgat
     8521 ttttcagatt tatctaaaac attaattgct aagcgcatac gatgcgagta aacagtatta
     8581 tgaattttga gtgtgccttc aacgatttga ctttctccca gactgaacga tatttcataa
     8641 gatatttctt ccatttcggg tttttcagat aaacgatctt ttaaatcttt aggaagttga
     8701 gcttttccgt aaacaactaa tgtttcttgt aaagaatctt tatatacttt agctgtttct
     8761 aattttagag atagttctgt atctccttct aatggtgaat aatcatacgt atcgccatca
     8821 tttgctgaat cgatcaaatg aacgaaatca agatattgtc tatctccaac tatcaaattc
     8881 aattgcccat ctttaaaaga aagtttatat ttattgttac ttatctcagt tttgtctatc
     8941 tcgattgctg tttctaattt ttcattattt ttctcaaatt caacaacagt gtatcctagt
     9001 gctggcacat ttacatttat ttttatgtca agttcataat aagctggttc atcaaaatat
     9061 tcgaaacctg tagcaatcat ccgataagca tttttccgtt ctggaaaata aacttctttt
     9121 tcaatgatat tatagggact acctttgaag tgtattttct ttgaatcagc tactacatgc
     9181 acgattttcg atccactata attctcagtc gttgtgttga aaacaaggac ttcattcgat
     9241 tttagcttta atgcatcagc catccgtttt acaataaggt tttcgattcc gtcagctatc
     9301 tcattagctt ctttgattcg atggaaaata tcttccgcaa cattatctga aacacagcca
     9361 ccaatactat catgagcctg gttttgcaaa atctttttcc ataaccgacg aagtaggcct
     9421 ttactgatat ggataccatt tctttctgca atcagcatga gcggctcaac tcgttttaga
     9481 attttttgtt ccaatttaaa attttctatt ttcatttttg ttcttactga gccaatagaa
     9541 cgatgaacac gagagtaaac cggctgacga agttcacctt tatattcagg taattctttt
     9601 ttgtttctga taacttctac ataagaagga taatctgaaa taatattgtg gaatttactg
     9661 tatttattca acttctctac gacttgatca aaatttaaaa tcattgactt ttgatctaca
     9721 cccgaaggta ctaaaatatc tttgtttttt ccttgcatat gaagcatttc aactgcttca
     9781 tccaactttt cttttgcaaa ttcaggaata tctttaattg cgtctaaggt catcattcca
     9841 gtcatataac caaatggaaa attcatggca taaacaaatt tatctcccaa tcctttccag
     9901 ataaaataag gagatggaac catcgtttca aagtttaatc ctcgccaaca catgaaatta
     9961 tcgatttcta cttgatgcaa aagtgtaggc aaattcgcat tgaacccaaa cgtgtcaggc
    10021 aaatacccta ccatcattgg atctccatat ttaaggatcg tatcactgat tccaatcatt
    10081 aagtttctta aaattgattc agcatccact aataaggcat ctgtttgtgc ataccacggt
    10141 cccacgaata gttgtcctgt tttcatcagt cgttggataa ccttaagctt ttgcggattg
    10201 atctccaaat actcatcgac gattgaagtt tgtccatcta aacaaaagtt tactttaggg
    10261 ttcctttcca attcatctag aacttcagta aagacctgat cagacaatac gatggcatct
    10321 tcatttgtaa aataccattc tctgtcccag tgcgtgtgtt gaattatatg tgcatttacc
    10381 atatcagatt ctcctacttt ccttcttcta ctgaatttac ttgttgttct tttttatact
    10441 tgatggattt tagtgcattg accattaacg ctgtaaccac tcccccaata acaattgata
    10501 ctaaatataa aggtatattc gtaacaaaag gaattgccat aaaggttgaa agcattgttt
    10561 cttgagtaac accaaaaaat ccatttagac cacatgctac tgcagtgccc accacgatgc
    10621 ttggtattac tcgtaaagga tctttcacgg caaacgggat cgtcccttca gtaatcccca
    10681 caaggcccat aatcatagct gtctttgcat tatctcgttc cgattcatca taaagttttt
    10741 tgtttaaaat tgttgctaaa aacattccaa atggcggaat agcaattcct aataacatgg
    10801 ctgtactagg tccaaagctt ccgcttgaaa aagaagcaac cccaaaagca tatgcgattt
    10861 tattgatagg tcctcccata tcaaacgcca tcattcctcc taaaatagcc gacatcaaag
    10921 cagcatttgc tcctgacatg ccttctagcc aattagtcag tcctgttgtc attgcttgaa
    10981 taggcgtagc tagtacatac cataatatga ttccaacaac taatgtagta acaaaaggaa
    11041 tcagcataat tgattttaga ctaataactt gcatcggtac ttttactttt ttcaatgcat
    11101 tagctaaata gccacaagct aatccagcaa ccattcctcc aataaatccc ataccaagat
    11161 tttgacacat catcccagtg ataaaagctg gtgctagacc gggacggtca gcaattgaat
    11221 atgcgatata ggctgaaatc atcggtacga taaactgtaa tcccgtctga ccaatcattc
    11281 gcaggactcc ccagaatgta tccgtctggt cccaaacacc tgtgccacca aaaatgactg
    11341 caatagaagt tagtaaccct gctgctacga caagcgggat catataggaa accccacttt
    11401 ggaaatgatt tagaatttct cttccttgtt tcttgaattc cattagtaac tcccccacga
    11461 tatatttttt atttgttaat taatgcaatt gcttcatcaa tcgtctcttc accatctttt
    11521 actacctctg aggtccctac tgttaaaatc ggtttaccgt caaagcgacc tcgttctgaa
    11581 acatttgtat ctactgcaaa tatgacaaca tctgcctcgg aaacatccga aattgaaagt
    11641 ttgttttcta tccccatagc cccctgtgtt tctactttaa tatctaaccc tttcttcttc
    11701 gcaaactttt ctaaattagc ttttgccata taagtatggg caatccctgc gatacatgct
    11761 gttactgcta ctatttttgt catatgaaat tcctcctttt aaaatgatag agcttttacc
    11821 aattcttttt tatttctcga atcttttatt cttttaacaa actcgtcatc catcaatctt
    11881 ccagataatt ctgctaaaat acgtaaatga ttagtacctt tatcagtgtc ttttattgct
    11941 aacaaaaaga cgagatcaac tggttgatca tctaatgcgc cccattcaac agaccgcttt
    12001 aacttggcta cagccactgt tgattcaatt actgctaggc ttttgccatg aggaatggcg
    12061 atatttttcc caattcctgt ggtcatatgg ttttctctgt caaatacgtc tttttgaaat
    12121 tgtttgtggt cagacacaaa cccattagtc tctaacaatt tcgataattt ttcgactaca
    12181 ctttttttat cagatgcatt taaatctatc actatattat ttatattgat tttttcactg
    12241 atcatttata agccttcttt caatatataa actaattttt caaacgacga actcgttttt
    12301 aaatttgcta ataactcatg attcgaagcg atttcattga atgtttcata aattttattc
    12361 aattcaggtt ttactttttc attaagtgca aaaaagaaaa cagaatgaac aagtgtccca
    12421 tcccaatcaa ttccttctgg attaacgtag acacagatta ctggcttttt tataaaatct
    12481 acttcgatat gcggcatagc aaaatcattc atcgctgttg aagatagttt ttctcttttg
    12541 attgctccct ctagtatccc ttctttcgcg taattttctt ttaccaattt tttggtaatt
    12601 atatttaaaa cgttcacttg attatccttt atttggtggg taaaaaagca aaatcgttta
    12661 tctaataaat ttagaaaagc atcttctgtt tgttcattta actgtagata ctgttttact
    12721 tttctgacat ctgcccctaa catcaaagga ctaacaacaa taacaggaaa atttgtattt
    12781 tcaatcggaa tcgtgcttat aacaagttca tcagtgactt ttctatcttg tagttcattg
    12841 atgcttagta catcttctat ctcaaccata tctgcaaaac cgttatttat tctttgctct
    12901 aataattttg atgttccata tccgctacta caaactaata tcactttcgt tttgtcaggt
    12961 ttgtaacgat ccaaaagtga ttgaatatgg agtgttacgt aagcgatttc atcttcatta
    13021 aaatgtatac agaatctctc ttcgatttcc tctaataaat ctacacttat cataaatgac
    13081 cttttaaaag aatatttgat tttgtccgta taagggttat agatatttac tcccaatttg
    13141 agtcttttaa ctgctgcatt gagatgtaat actagattat tgatcaattc ttcatcagga
    13201 taaaattcat ttaatttaat tcttactaac tcttgtagct caacacccat atctaaatag
    13261 gaaagcttcg tttgatctaa gtcgtgattc atagcatttt tttcaatcga agtaatgtga
    13321 atgtcaacat aagcttgttc atctattggc acaataattg aaaatgcttt ttcaatcatt
    13381 ttgattaaaa taagtgtatt ctttgagatt tcttcttcag aattattact acgtgataag
    13441 aaattgcttg tttcaatcct ttctatcgca atagcaaggt ggatagctaa agtttgaaat
    13501 tcataatctg taaatttcaa ttgtgtctta ttcatgtatc gattaagcaa ttcggctact
    13561 tttttcaata tgtccttatt taagaaatta ttcgcttcac caatgaaatt gatattcaag
    13621 tctaagagat ctgttttact gttagatgct gtaaagccac cccaataata gttcacaact
    13681 tctgacatca accttctttt acttttttca tcagctttga tgcgcacgcc atttcgatct
    13741 gaaactaagg acatcccatg cttgtttagt ctttttctaa catctttcat tgtgctttct
    13801 accgtcgacc tgctcacgta taattcgtca gcaagatttt gaatctttag agaatcatta
    13861 gtattcaata atttagcaaa cacataagtt agacggtcct ccggagagtc tgcgatattg
    13921 attttatgac tggataataa atttcgaagt gtttcttttt ttccaataag cgaaacgcct
    13981 tttttaggtt gaaccaacat ttgtacatct ggcatttttt ggattatttc tttcaaaatt
    14041 aaaatatctt tgctaattgt tttcgtagat acatataatt gttctgataa ttctttgtaa
    14101 gttgtgtgct cattttcaat caaaaagtat agtattttca tttgccgttc gctaaattcg
    14161 atgcccatta tcttcacctc ctgaatacag cttaagtgta caattattat ctctattttc
    14221 atacagaaga aagttcttga ttaatgtaga agtttctaat attcttgatt atttcaaatc
    14281 aataaaaaag cctgcaatat ttatatcaca ggcttttttc tctattaaat atatataaga
    14341 taatacattt ttctactagt tctcactaat cttgtataat taaaccctcc gcaacttgac
    14401 ggagatggag aagatgcccc cgtctaatct agcggtcatc tctcctccca ttccttccac
    14461 caattttttt gacaggtaaa gcccgattcc gctagattcc gtatttgtgc ggctggtatt
    14521 ttcggtataa aaacgctgga agactttttc aacagaggtt tgcgttcctg gtttgatagg
    14581 attggccact gttaatcgaa tgaatggatc ctcttttgaa aatgtcacct ttgcttgtcc
    14641 atctgcataa cgcaataaat tctgtatgat attttgcagc actcgcagca acatgagcgg
    14701 atctgctaat acagttgtat ttatcgagtg ttcagcgaac gctacctcga tatttttctc
    14761 agaaaattgt tcataaaaag agaaaaatac ttcttccacc tctttagaaa aagaaagcgt
    14821 atggagatcc atttcttttt gcttcgtctc tattcgagca agatcataaa actgatccgt
    14881 catactgagt aatcttgcaa cagattgttt gatgatcgtt aaatacttct cccgtttctc
    14941 cgtatcctct acctgttcca gtaattgtac ataaccgtta atagcagtca aaggagttct
    15001 gagatcatga gaaaggccga cgatcgatag tctgatgttt tcttccattt cttgaaatgc
    15061 ttgtcctttt tgttcatttt catccaacca tgtattcagt gcatctacta ggtccattag
    15121 attcttttct cgaaattcga ctgaaagacg accaccatat tcttgtcgtt cagaaagagt
    15181 ggcgattttc ttagttaatc tcttgatttc cctttgcagg aagaaaataa aacttcccat
    15241 aaagataaca acgagaagca acagccattt aatcaaatct aaaccccact ccccacaccg
    15301 tatgaatata cacagcatgt gcctttgcta gcttccctcg aagattggaa atatgcatat
    15361 tcactgtttt ctcactatca aaatatggtt cttgccaaac tgtttcataa agatttgctc
    15421 gcgaaaatac ttttttcgga tgttctaaaa aaagcttcag cagatcgaat tctcttcctg
    15481 tcaaatggac agccacacca ttcacattta catccctcga ctctttgtcc agtaagattt
    15541 ccttgtaata gatgcgttgc gacagatctt ttacttggac agaagtctga tgacgaagtt
    15601 ggatgccgat tcgtaacaaa agttcttcga tatcaaatgg tttcgtcaca tagtcatccg
    15661 ctcctgctgc caacaaggaa actttatctc tttgatcgct ttttgctgaa agaacgatga
    15721 tcggaacatt cgatgtctgt cggatttctg ctaacaatgc ttcacctgaa agccctggaa
    15781 gcatgagatc tagaaggatc aaatcgacag gatatgtgct taacaataat tttccttccg
    15841 tacctgaata agcatcaaga agtttgtatc tctgttcaag aacctcctcc agtaaggaat
    15901 gaacagcttc atcgtcttcg ataatcaaaa tagtttccag caggaccacc tacttaatgt
    15961 cagatttttg gaaaataaac attcctagag ctgatgatac cacgattgtt actagactga
    16021 ccagaatcat tttcggtgct tgcgaataag ttgcttgttg cgcaaagtcc gtatacatga
    16081 gccacgtttc cgtaaaccac tccgttagaa attccaagcc tttgattttt gcgaaaacac
    16141 cgggaatgaa ggccgttccc atgatataaa gaaaagaaag aagcaaactg acactggtac
    16201 ttctagtgat gaatgtcaaa gccactggaa cagataatgt ccctaatgtc agcagaagca
    16261 atacaggaaa tacttttaga acataatttc catagttttt gattccctca acttcaccaa
    16321 atccgaaagc aagtgtgtaa gcaagcatgg ctagtcccgt gatcaaaacc acgccaagtg
    16381 ttgtgatcaa aagcgtaacg agaagtttcg acacataata agtcattcga ttccgtccta
    16441 aagataacgt attgcgaatc gtaccgtttt gaaattcttc cccaatataa aagcacgcaa
    16501 atgctgcgaa agccagaaga atccatgcag ccattttttc taccattaaa atccctgttg
    16561 caccgttgat gactgtatcc gttgcttggt ttaattcatt gccgtatttt gtatgcatat
    16621 accattcgtt ccaagaataa aaaagcggaa gtacgaccat cagtcctagt gagacccaca
    16681 ttttacggct cttccatact tttatttttt cagcagttaa tagattaaac atgtggattg
    16741 cctccagtta attgtaagaa ataatcttcc agtttttgcc caactaggtg aataccatcc
    16801 acaggaactt ttgcttctac caatgcttga ttgatggctg ctacttggtc aattccttca
    16861 aaaagctgga taacacggct agattgtaca aaataatccc tataaccaag agagtcgaga
    16921 accgttactg cttgttccgt cgccgaagtg tcaattttta taaattgacg agattcttgt
    16981 gccaattctt ctttcgataa ttctttgatc aagtgtcctt catgcaaaat accatagtga
    17041 gtggcgatct gtgacaattc atctagcaag tggctagaaa gcaagacggt gatgccccgt
    17101 tcttttacca aacgttggat gatttctcgc atttctgcca tccctgctgg atccatccca
    17161 ttcgtcggtt catccaaaat caaaaattct ggattcgtga tcagtgcatt tgcaatagct
    17221 aaacgctggc gcatacctaa agagaaattc tttgctttct ttttccctgt attttccaat
    17281 cgcatcattt tcagcaggtc ttcgatttct ctatccgaca caccgccgtt cgctgcttgg
    17341 atacgtaggt tatctcttgc agttaattct ggataaagag caggggtttc aatcgattga
    17401 cccatacgtc gtcttgctgc ctgcaaatcc gtcgttccaa ataattcgat cgaaccttca
    17461 tcaatcgaga tcaaacccat aatagttctc ataaaggtag acttacccgc tccattttcc
    17521 ccaatcaatc catagatcat tccacgtttg atttcgattg atactttatc taatgctttg
    17581 gcagcaccgt attttttggt tataccagtt gctttcaaga ccgtttcatt tccaaaatcc
    17641 atacttatcc ttctttctta ttgatgagtg aagtatagtt gacgggtata aagaaagcag
    17701 ccagaaatgt ataaagaaag tgtaaatttt ccaattgaat gtcaaaaaaa acaatcggac
    17761 aatacgtgtc tttcctatta tcaggcagtt tgttatacgc ttgtatcagt ttcctgattc
    17821 tttcttttcg accaaagtcg tttctctgta ttcctcttca ctgaaaattt ttgcttcttt
    17881 cagatcaagc ttgagtaatt ttaccagacc ggttataagg ataactatag caagactccc
    17941 tcccagaaaa attagactgt ctcgcagacg tggttgcgtt gtcttccaat caaagagcgc
    18001 accaaacagt gctaatccaa gtggactggc caccatggta atcgttgtta tcgttgacat
    18061 aagtcgtcct tgtatatgcg ttggaacttc tcgctgaagc cagaccatat aaaaagcaaa
    18121 agaaaacatt aatccttaaa aacggactta tgaaaaataa tacatgccgt ttgggattta
    18181 caattagcta ccaaataaga ggttatgaca aaagactttt cacaatccct tcttccgaat
    18241 aaacggtgtt ctcctgccaa ctcctcggtc acaagtcaca atatttatca aacatgagaa
    18301 gcaatttttg taaattcctt cttatttctt gaagctgacc acttctgtca caacctcaga
    18361 agcggataaa taatattgtt tgatgatact ttatcttttt cccgtttgtt tatagatcga
    18421 atgatattat agaccaagaa tcctacctat taacatagct aaaaacacaa aaaaggcact
    18481 ttccaaattg tggaaagtgc cagaaaagtt aggattaagg taactgcgct cagcattgct
    18541 tcgcccagta cgattctcgt aactcgtgaa ctcgaacgat aatcgcaatt aacgttttga
    18601 gaactgtgaa gctttacggg c
//
LOCUS       DAYYCY010000040        18510 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000040 DAYYCY010000000
VERSION     DAYYCY010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18510
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.040"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(110..565)
                     /locus_tag="ACHAZB_001123"
     CDS             complement(110..565)
                     /locus_tag="ACHAZB_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8613812.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(719..1927)
                     /locus_tag="ACHAZB_001124"
     CDS             complement(719..1927)
                     /locus_tag="ACHAZB_001124"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HFM8613813.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNEGAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2039..2371)
                     /locus_tag="ACHAZB_001125"
     CDS             complement(2039..2371)
                     /locus_tag="ACHAZB_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFM8613814.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2519..3394
                     /locus_tag="ACHAZB_001126"
     CDS             2519..3394
                     /locus_tag="ACHAZB_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM8613815.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3476..4057)
                     /locus_tag="ACHAZB_001127"
     CDS             complement(3476..4057)
                     /locus_tag="ACHAZB_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ReoY family proteolytic degradation factor"
                     /protein_id="HFM8613816.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4063..5322)
                     /locus_tag="ACHAZB_001128"
     CDS             complement(4063..5322)
                     /locus_tag="ACHAZB_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFM8613817.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5324..6370)
                     /locus_tag="ACHAZB_001129"
     CDS             complement(5324..6370)
                     /locus_tag="ACHAZB_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8613818.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(6605..6880)
                     /locus_tag="ACHAZB_001130"
     CDS             complement(6605..6880)
                     /locus_tag="ACHAZB_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFM8613819.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(7123..8433)
                     /gene="der"
                     /locus_tag="ACHAZB_001131"
     CDS             complement(7123..8433)
                     /gene="der"
                     /locus_tag="ACHAZB_001131"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFM8613820.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(8615..9844)
                     /gene="rpsA"
                     /locus_tag="ACHAZB_001132"
     CDS             complement(8615..9844)
                     /gene="rpsA"
                     /locus_tag="ACHAZB_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFM8613821.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(9979..10659)
                     /gene="cmk"
                     /locus_tag="ACHAZB_001133"
     CDS             complement(9979..10659)
                     /gene="cmk"
                     /locus_tag="ACHAZB_001133"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFM8613822.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(10708..11343)
                     /locus_tag="ACHAZB_001134"
     CDS             complement(10708..11343)
                     /locus_tag="ACHAZB_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1386/EF1546 family surface-associated
                     protein"
                     /protein_id="HFM8613823.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(11409..12812)
                     /locus_tag="ACHAZB_001135"
     CDS             complement(11409..12812)
                     /locus_tag="ACHAZB_001135"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFM8613824.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(12799..13830)
                     /locus_tag="ACHAZB_001136"
     CDS             complement(12799..13830)
                     /locus_tag="ACHAZB_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8613825.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            13861..14082
                     /locus_tag="ACHAZB_001137"
     CDS             13861..14082
                     /locus_tag="ACHAZB_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HFM8613826.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(14247..14984)
                     /locus_tag="ACHAZB_001138"
     CDS             complement(14247..14984)
                     /locus_tag="ACHAZB_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HFM8613827.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(15137..16477)
                     /locus_tag="ACHAZB_001139"
     CDS             complement(15137..16477)
                     /locus_tag="ACHAZB_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFM8613828.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(16714..18237)
                     /locus_tag="ACHAZB_001140"
     CDS             complement(16714..18237)
                     /locus_tag="ACHAZB_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFM8613829.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
BASE COUNT         5220 a         3936 c         3120 g         6234 t
ORIGIN      
        1 aaaatcttgt cacagtccct tattctgaat aaacggtgtg gaagagacag cctatcagta
       61 gtaataagac acatctcttt cacagcctca tttttgcgta aaaatcgatt tattcgaaaa
      121 attccccgta tacatcttct acacgttgat ctggattgat cacgatatac aagttacctg
      181 ttttttcgct gatttttgtt gattgatatt gattgtctaa tccttctgtt cctttatcta
      241 gatatgggaa gtacacaaga tcaggtgtgc catttcggta aaggatttcc attcgctcga
      301 tatcttcgta tttcaatgcg cgattaaaca tgcccatttc cagtccgcct aggttgatat
      361 catgtgtcgc aactgcgtcc ccttcatgga agatttcgat tttaaatcca gcacacggat
      421 ggatttcaac aaattcactg ccattgattc gtccaaaact tgttgtgatt tgtttgatcc
      481 aaagatcgcc gatatgacgg cggttgatcg tccaggtttc tccatttcta aaataaaatt
      541 ttagcgctgt catttcttta ctcattaaaa agactccttt atgatgtaat ttattcttgt
      601 actacctcag tttagcacaa atttatgtaa gtggtcacat cttatgtttt ttcttctaat
      661 gacgtatctg cccactttaa gtagccaaca aagctgagca gatacagtct agtgtttttt
      721 aaatcaattt ttctttcgat aaaaaatcca aaacctgttt tttatcattt tttattcttt
      781 gttctacgac ggctttttca gctaattgca aagcttcact aacccactta ccaggttttt
      841 ttccagtttc ttctagcaag attttaccat ctactgctaa ttcgcttaat gacttgatag
      901 gtaaacgatc ataggattct cctacggcat ccatctgagg tgtttcgtca aaatagatca
      961 tcaattcttc tacttgcagt gcatgttttt tgcctaagaa gtacaattcg aatggttcca
     1021 aagctccagt ttctcgttgt cgtagtcctt ggacaagtgc ttgaacgtct ttaatcattt
     1081 ggttagaaca cttccacgat ttcaaaaagg aacgaatctc attttctttt agatccagtt
     1141 ggctgatcaa taatgcccat gcttggctct cttctttcaa tgggacggat ggtaattcag
     1201 caaaacgtaa aagtgcttcg ccttcttctt tcaaaccggg acagcaaatg tagcactctg
     1261 tttcaataaa tgcatggatt cccgcggccc cttcatttcc caagagcatc ttgatgaatt
     1321 ccacatttat acgttctaca gatattttac ctaatagctc gtggttttct ttgatggcgt
     1381 agaaggtttc ttcctctaat ttgaacccta gttgactgac aaaacgcaac ccgcgcatca
     1441 ttcgtaaggc atcttcatgg aaccgctcat gaggattgcc tacagctcgc aagatctgtt
     1501 cttctagatc ttgcagacca tcgaacaaat cgatgatttc tccatcttct ttcaaagcaa
     1561 acgcattgat cgtaaaatct cttcttttca aatcttctgc caacgaacga acgaattcga
     1621 catgatctgg tctacggtag tcctgataag ttgattccgt ccgaaatgtg gtgatctcat
     1681 attgttcatc attttctaat accaacacag ttccatgttc gatcccaata tctatcgttt
     1741 ttggaaaaag ctgtttgatt tcttctggaa atgcgcttgt tgcgatatcc acatcatgga
     1801 ttggctggtt cagcaagata tcacgaacac ttccacctac aaaataagct tcatatcctg
     1861 cattctctaa ttttttcaga actggaattg ccttttggta ttccagaggt aattgtttta
     1921 atttcatttt tccaaccttt cctgcgtgtt tcttgtcctg agataaagac tcagttgcat
     1981 ggtgactgtt tgacaagtcc gtttcagagt cttcaaggcc aggaccactt catcttgttt
     2041 actcgtcctt acgctcaaag cgattatggt ctctttgatt gaatttttcc atgttttttt
     2101 caaaaacttc agttaagtcg atattcaatg aattagccat gatcatcgta acgaataaaa
     2161 catcaccaag ttcttctgct accgagttaa ctggttcaga agcctttttg cttttttctc
     2221 cataagtgtg gttgacttct cttgccaatt cacctacttc ttcagttaat cgagccatct
     2281 gagctaatgg agagaaatac cccaccttga attgctggat atattcatcc acttcctgct
     2341 gcatactgta taaagaacgc tttttatcca tctttttccc tccaaaacta tcttaccaaa
     2401 actttaacgc aaatcctaga taagagcaat tgagaaaatc gaatttccat gctacattta
     2461 ggaaggaatt tctaaatgga aattctgtga aaatacataa ttgaaagctg gaattcatat
     2521 ggtcaaacga aaaacagtaa aagaagttct ttttatcatt ataggaacaa gtatttatgc
     2581 atttggtctt gtttacttga acatcgctaa tcatttagca gaagggggag tctcagggat
     2641 caccttgatc ttaagagctt tatttggtat tgatccagct tatacgactt tattaatcaa
     2701 catcccattg attttactag gaggaaagat cctcggtaaa cgttccttgg cctatactgt
     2761 tctaggtaca ctttctttgt cattcttttt atggatctgg caaagggttc cactagaaat
     2821 caatttacag catgatctac tgatcgtttc tctacttgcg ggtttagttg caggtgtagg
     2881 aagcgggatc gtctaccgca tgggtggaac aacaggcggc agtgatatta tcgctcggat
     2941 tttagaacaa aattacggca tcactatggg gcgttcccta ttggcatttg atatcatcgt
     3001 cttacttgct tcattaacct atatcgactt gaaacgtatg atgtatacgt tgattgtttc
     3061 gtatgttttc agtcgtgtga ttgattcgat cttagatggc ggatattctg caaaaggtct
     3121 acttgtcgtc tccaacaaaa gcgaagagat tgcaccacta ctgatgaccg gtctgcaaag
     3181 aggtgtgact ttcctttctg gagaaggcgc attctctggt aattcaaaaa aaatcatcta
     3241 tatggtcgtt agctctagtg aattaactga agctaagagg atcgtccacg agattgacga
     3301 aaaagctttc ctttcaatca tcaatgtgca tgaagtggaa gggcaaggct ttacttattt
     3361 gaagccacag acaagattac tgaaaaaact ataagaaaaa agacaaagat aggctatgag
     3421 gaaagattcc acatccagct gacctttgtc ttttttagta ctatactatt tttaactaat
     3481 tgttatgttc acttacccgg ttacttttct tttgtgtctg caaaaggatc tttcggttaa
     3541 cttcatcaga taatttcaaa aaagcatccc tatctccatc ttctagtgct tgatcgattt
     3601 gagcataaag catttttagt tttgcctctt cttctttttc ttgaaaatat cgttcaatta
     3661 attcagtatc ttcttcactg actgaagaat cccaagaaga aaatggattg tcttctaaga
     3721 tagatagata ttctcttgtc tgccaagcat tttcgaatat acattccaca tataaaggct
     3781 ttttccaatt caaacggatt tcatgaaaaa tctggtctgt gtccgtaaat tcttttccct
     3841 gcaaaaacaa tttcatgggt tccccttcaa cagatagatc actgatctgc agtcctcgat
     3901 ccgcaaaatt cgccccttca acaaagtgga cattgcttaa aatcgcttca tgattggcaa
     3961 gataattcaa gatccaaaaa acttctcttc ggctaaaaga tacatgattg accatccacg
     4021 ttaaaaaatt cttcttttcc cgtacatcaa ttagcatttt tctcacctct ctgttaggtc
     4081 atctaataat gacagtgcct ccatatctcc tggttcaagt gttaaataat aagtcaataa
     4141 ttcttgcgct cttttcagct gcccttcttc tcttaagaaa aaggcatact ctttcaagaa
     4201 atctggttca tgtgatagtt catgataagc ctgttcatag tgtacagctg caacggtaaa
     4261 gtcttctaat tcattataag catgggctag gttccattct gcataaggat tagcagtttc
     4321 ttccatctga ttgatcactt gtatcacatc ttcatatctt tcatcatcca agtaaagatt
     4381 acttaaagta agtaatgttt catcttgttt gtctcctact tctaaagctt tcagtaaaag
     4441 ttcttctgct cgctgtctat catgcagacg gaaagcattt tccgaggcca ggtgataaag
     4501 ttcgacttgg taaggatttt ctttgattcc ttcttctaac actgtttgtg cttcttcaat
     4561 catttcttct tcttgaagag cttgtcctag atatagatat aacgattgat aatgcggatt
     4621 aacttctcga agttcttcca gatagttgat tgcctgctga ttttctttca attgaagatt
     4681 cgtaaaagct agctggaaca gtcgatcatc tgtcctttct tctttcagtg cttcttctaa
     4741 aggtacgata gctgcttcga attctccttg catacttaag gattgtccta aacgttcctg
     4801 catcgagatt cctgaaattt catttatccc gcttgccaac aatgtttcat aaaccactgc
     4861 tgcctctttg aaacgatcaa ctgaaaaata caattcagct agcgcaaact ggatcaatgg
     4921 ttcatcagga agcaacccag ctgcttcttt caattttgct tcactcactt cagggattcc
     4981 gataacttgg tacaaatcag ctatcgccaa taagctttgc ggatagtaat cactttgctt
     5041 atccactttt tccaggtatt caaaagctaa gtcgatctca ttatcttcga ttgcaatttc
     5101 tgctaacggg atgttcagtc cgtcattttc tgggtattgg ttgatcagtt gttcaaaaat
     5161 ctgttttgct tcgatcaaaa agccaatagc cagcaattct tcgcctagtt cagctaatat
     5221 atctgctggg tcttctttta atgctttttc taaaaagatt tgtgcttgtg acaagtcttc
     5281 atctgataaa gcttgcaaca ttttttcgct atttgtattc atttcaattc cctcttatct
     5341 cactgacgat ttgttgaaat accgtcattg ttctcaataa ttctttttcg tttggatgtt
     5401 ggtatccgtc tattatacct attttttcta aaacaagtat ttttttgctt ttttctgcca
     5461 aaaaaagaac tttttccaaa tactccatct ttgaaatctg ttccagccag tggattggat
     5521 agccaagcaa gtccagccac acaaaaaagt tttttagttg gaaatcaaaa ggatatgctt
     5581 ggagattcca cacgaaatga aacatcattc ctaaaaattt tttcatacta gcagaaagta
     5641 gatggccatt ttcggtcaga taaaaagctt gttcaaacag tttaccataa tttgacaggt
     5701 tttctgcctc taattgataa aaatagatca tttcttcgat catcccggca aacggccgag
     5761 tcgttaattg ctttttattt ggatagtttt tatacagatt ttgcaaaaaa cgatgatcac
     5821 aaatcattcc gcaacagata aaagtcaaaa agtcaatctg ctttccgtcc acctgccgct
     5881 cgctgattgt ttgatcataa acgatgcgtt ctggcagatt gatggtttgc agcactacac
     5941 gatttggtag ctgcaaaatc gtccgctgcg gtgttaatgc tcggcttaga gaacggatcg
     6001 atacagggat catccatagt gatgaatcaa gggttgtatg tttttgaaaa aaacctgcca
     6061 gatccatgat cccttcattt ccaaatccga tgataaacat ggattgattt tcgggatagc
     6121 gtttgacgaa atcaagcagg ttctttaatt ctgttaaatg attgcattga gtgttggcac
     6181 aaatatacca gtcattgttt gcttgatttg caaataacgg gttgatctta tctgcaaaca
     6241 aatcataata gcgctgattc gttaaaaata agattggctg atcaatggaa agttctctta
     6301 gctgactgac tagcgtttct ccgtaaaaca ctttcgtcaa cgcatcttct acttgataat
     6361 caagttccat acgcatccct cctcttctta tttttatttt accattagta agcaaattct
     6421 agaaaagata agagcaaagc tcacctcata acttggaaat aaataaaaat taaataaaat
     6481 acatgtgttt tctatgtatg acatacggat tcggaatctc tgtctcttat ttattccttt
     6541 tctgctagga aataaaaaga aaagcacttg tagccctaga gccacaagcg ctttttcaac
     6601 tgtcttattt aacagcgtct tttaatgctt tacctggttt gaatgcaggt actttgctag
     6661 ctggaatttc gatttcttca ccagtttgtg ggttacgacc tttgcgagct gcgcgttcgc
     6721 gaacttcaaa gttaccaaaa ccgattaatt gaactttttc gccgttagct aaagcatctt
     6781 gaatagttga aaatacagca tctactgctg cagttgcatc ttttttagtt aaatcagtag
     6841 ctgaagcaac tttttcgatt aattctgctt tgtttgccat ggatagattc acctcctcca
     6901 caagagatat gatagaaata tcatttagtt aaaactttct gagtggtcca gaagtttaaa
     6961 aagaaatatt gattaacttc aatattctgt tatcacgata tcatatgaac cgcttaatag
     7021 caaggttttt accaattttc acatgctttt tttagtcata gaaacgtaac aaacgaaact
     7081 gtctttttct tttgaaaaaa aaacgtcgat atggtaaaat gcctattttc tcctacgagg
     7141 gataattcgg atggacgtcc cttcaaaagt aaatgctttt ctgatttgat tttctaagaa
     7201 acgttcataa gaaaaatgca tcaattcctc ttcattgaca aaaacgacaa atgtaggtgg
     7261 tttgacagcg acttgtgtag cataaaaaat cttcaggcgt tttcctttat ctgttggtgt
     7321 tggattgatt gctacagcat ccataatgat atcattcaac acagccgaag agatacgaag
     7381 attctggttc atacttactt gttcgatcat ttcaggcaga cgttccaacc gttgtttagt
     7441 cactgcagaa acaaaaacga ttggagcgta atcaagatac cggaattcat cacgaatctc
     7501 ttgttcgaaa tcacgcattg tattggtttc tttctttaca agatcccatt tattcacgac
     7561 gataataatt ccacgaccag cttcatgggc gtaacccgct actcgcttgt cttgttcccg
     7621 aatcccttct tcagcattca gtaccatcaa aacgatatca gaacggtcaa tggcacgcat
     7681 tgcacgcatc acgctgtatt tttcagtatt ttcgtatact tttccgcgtt tacgcatacc
     7741 agctgtatct atcattaaga atttctgacc actttcagaa acgaagtggg tgtcgattgc
     7801 gtctcgggtc gttccttcaa tatccgaaac aattacacgt tcttctccta aaatcgcatt
     7861 gatcaaagag gatttaccaa catttgggcg accaatcaaa ctaaacttaa tcgtgtcgtc
     7921 gtcctcttct tcagtctctt cagagaaata cttcactgct tcatctaaaa tatcaccaat
     7981 ccctagtcca tggcttcctg aaacaggata aggatcgcct agccctaagg aataaaattc
     8041 atagatatcg tttctcattt ctggattatc tactttattg actgctaaaa tcactggttt
     8101 attacttctg tataaaattt tcgcaaccat ctcatctgcg tctgtcacgc cttcgcgtcc
     8161 gctgactaca caaatgatta catctgcttc ttcaatcgcg atttctgctt gatgcttgat
     8221 ctgatccata aaaggttcat cgccaagatc aattccgcct gtgtcgatca cgctgaattc
     8281 tcgacctaac cattcacctt ttgcataaat acgatcacga gttactccag gagtatcttc
     8341 gacaatcgag atacgctcac cagcgatgcg gttaaaaatc gtagacttac cgacgttcgg
     8401 gcggccaacg attgcaattg ttggatttgc catattattt cctcctcaca agtttccaat
     8461 ctcacttagt ttaacgaatg cattgccaac tttcaagcat agattgtcaa ttaaacgaag
     8521 tatttttatt ccttattgaa atactaatcg cttttcaaaa gaaaaactga gacaaaagca
     8581 gctgctttca tctcagtcac agatacaaaa tcaattattt ttcagaatca tcggttgctt
     8641 tttcttctac ttcttctttc aacgcatcac ctaaaatgtc tcccatagta aatccagtat
     8701 tttcttctgg taattcataa gactcaacat tttttggttc ttcttctgaa gctggttttt
     8761 cttctaaagc tttgatactc aatgcaatac gatgttcttc tggatggatc tctaatactt
     8821 tcactttcac ttcgtcacct tcatgaagca cttcatgtgg tgtcgcaata tgtttatgtg
     8881 aaatttgaga gatatggact agcccttcta ctccagggaa cacttcaaca aaggcaccga
     8941 agcttgtcaa acgtttaact gttccgtcta atactgtgcc aactggagct tttgtatcga
     9001 tttctgacca aggtccaggc tgtgtgtctt tgattgacaa ggaaacacgt tcttgttcag
     9061 gattgataga caacactttg acatttacat catcattgac agccaataca tcacttggtt
     9121 ttgctacatg actatgtgaa atctctgaga catggactag accgtcaatt ccgcctaggt
     9181 cgacaaatgc gccaaaatca gtaagacgtg caacacgtcc actgagaaca tctccctcat
     9241 ggatagttga aagaatttct tttttctttt cttctctttc gttttccact accgctttat
     9301 gcgataagat caaacgattt tctgatggtt cgatttcgat gattttaaag ctcaatgttt
     9361 tgcctttata gtctgaaaag tctgcaacaa aatgatcttc caccattgat gcagggacaa
     9421 aaccacgtac accgacgtca acgactaaac cgcctttgac aacatttgta acaggtgctt
     9481 cgatgacctt gccctcttgg aaatctcttt cgatatcttc ccaaactttt ttagcatcta
     9541 aacggcgttt tgaaagtagg tagctgccgt tttctttatc tttgccgatc gtactgataa
     9601 cgactaaatc taatacatca ccgactttta ctacttcgct gatatcttct accggcaaag
     9661 ttgataattc ttttgccgga acgactcctt caactccagc cccttcgatt ccgacaatca
     9721 cttgtttgtc ttcaatgact aaaacttcgc ccttaacaac atcgccaact tttacttctt
     9781 gtacgctttt cattgcgtct tccattgact ggttgttctc ttgattttga tcaaattctg
     9841 tcataacact tgtcctccta accaacattc tgccgtaagt acggtactgt ttttagttta
     9901 attgattatt caaaaaaaat aaagcgattt cgttcgtttt ccttgaaaaa acaattgttt
     9961 ttcatttttt ttcttaattt aaaaaagaag ataacctttt ttcaaaacaa cagcttttat
    10021 tgctgcaacg acttctggaa tcgatttgcc agtcgtatcg atacgaacag catcctctgc
    10081 ttgcttcagc ggagagacgt cccttgttga atcataatgg tctctttcct gtatctcttt
    10141 cgtcagtgtt tcaagatcag tcggaattcc tttttcttgg ttctctttga aacgtctttc
    10201 tgcacgctca cttacgcttg ctactaaaaa gattttcact tctgcattcg gtaatacagc
    10261 tgtcccgatg tctcttccgt ccatgacaac gccaccgcgt tcccctattt ttcgttggac
    10321 agcaactaat tcttctctga cttttccatg tgcagagact tcagaaacag cttttgtgac
    10381 atctggttga cggatttcta aagtcacatc ttttgatgct atgaatacat gctgtccatc
    10441 ttcttgttgc gaaaaagaga taggatactt tttgatcagc gaaactagcc cttcttcatc
    10501 tgaaaaagat acattgttcg taatagctaa ataagttact gcgcgataca tggcaccggt
    10561 atctgtataa atataattca attcttttgc taatatcttg gcaacggtac ttttcccaga
    10621 tgaagcaggc ccgtcgattg caatactgat ttgtgtcatg atataactcc tttttctcag
    10681 aaaaaaagcc gcaaagcgcg gctattatta tttaattctt aattcttgac cagggtaaag
    10741 cataaagttg tttggatcta agccattcaa ttcaaacaat tgatcagtgg tgatccctgc
    10801 acgctcagcc acttgttttg gaccttcgcc tgcttgaaca gtcgttgttg aaccggctgc
    10861 agctgatgaa gaaggtgttg tttctgctgc attgctgctt gaatcccctg cttgataagt
    10921 tgcctcactt gaggaagtcg gtgttgtctc tgctgcaata ctgctttctg acgttacacc
    10981 tgtatcttga gacacagtag attcttccgc gattgtacta gattctactg ttgaagtctc
    11041 agttgactcc tcttttgttg tacttgtaga actttctact actgatgtcg aagtttcaga
    11101 agaagttgct gctgttgcat tgttgcttga tccatttctg atccagaaat aagcacctgc
    11161 tggaatagcg atacataaga ccagtaaaat caaaaatagc gaaagaaata atgtatttcc
    11221 tttcttttgt tgtctctgag aacttcttga tgaaacttca tcatctgtat cgtagattgg
    11281 ttgttcccat ggttcttgtg tttcgttatt ttctgtagat tgatgtctat cttttctgct
    11341 cactgtttta cctcctaaac tctatcgtgt gtattatacc ataggaaaaa gtgtcttgtc
    11401 ctaaaaaatc aatgttccat gtttttttta aataaattta gtaaaatcgt ttcccaatga
    11461 agttgttcag tcttagaaaa aagcttcctc tttggcagaa gttctgcttt tgctccatca
    11521 atatcacaac aattctgcgg cttttcggag agtttttcgc caaaataagc taagatgaat
    11581 tttctgcggc agttttcttc attaatatag cgaagcatct gctgcaggcg gaattctttt
    11641 tctttttcct gacgtttgag ccgttccacc caattttctg gtttttcaga ctgctggatc
    11701 agctccatcc attttttttg cagctcatca aagggagctt gctttgtggc atattccaaa
    11761 taaagctcaa aactttgtcg ctgttctcta gaaagttgat taaagaaata atggatacgt
    11821 tcatccccac tttgataaaa aagaattgct gcactttctt cctgatctct gcctgctcga
    11881 ccgatttctt gtacatagtt ttctagactg tctggcaaat cataatggac cacataacgg
    11941 atatcagatt tatcgatccc cataccaaat gcattcgtag caatgagaaa ctgcagctga
    12001 ttttttacaa actgctgttg aagttgtctt ctttgagctg catcaagacc accgtgatag
    12061 tatccaactg taaaacgttc cctgaataaa tgatacaatc gttcgacttc tttttttgtt
    12121 gcacaataaa tgattgccgc tccttgtgct ctttccatga agtgttcaag ttcttgttct
    12181 ttttgctgcg ttttccgaac aaaaagcgag atattttgcc gattgactga ttgtctgatc
    12241 tcttttggtg gtcgatcaaa tagtacatga cgtatatctc ttgctactaa atctgttgct
    12301 gtcgccgtta gtgccagtgt gaccgggttt cctaaaagct ttcggatttt tcctaactgt
    12361 tgatattctg gacggaaatc aactccccat tgagacacgc aatgagcctc atctactaca
    12421 tataacgcaa tattttgttt tttcagatgc tctaaaagag aaggattagt caacatttcg
    12481 ggactcaaaa acaaaaattt atattgcgat agatgacgga ggacgtatct tctttcagat
    12541 tgcgaaagta aactattgaa agcagctact cgtttctccc ctcgtttttg taattgcgtc
    12601 acttgatctt ccattagaga aagcagcggt gacacgatga tgaccagacc ttccattaaa
    12661 taccccgtca attgatagca aaggcttttt cccgttcctg taggcaaaat agcgagagta
    12721 tcttctccat caagcaacgc ctgaataatt tcttcttggc cgggacgaaa tgaatcaaat
    12781 ccaaaatatt tttttaattc atttattaac attctgtatc gcttcctttc tcaatagata
    12841 gatttgatac aaacgaaatt ctccataatc taattgtcca tctacattcc attcgctaaa
    12901 gcgccactct cgcacggaat ccttatttgg ttccaaacgt tcgattgttt ctgcagagag
    12961 aatccactcg tatggaaaat catcaaaaaa caaggcccac tcaataagat gatctgttac
    13021 tgttcctctt tttaaatgac ggattgccat gatctggtca attgtctgac cattcataaa
    13081 catctgcttg gtgatcatca tgctttgatt gaaattttgc tgtaataatg aatcgatcaa
    13141 agcgtgccaa agcgaatctg gccgtttttc gatttgtgct aacaaaaggt ggatacatct
    13201 gctttgttgc agataaacct ctgactcatc attgtttttt gccagctgat aaggaagcag
    13261 ccctgttcgc tcatttccag ataattggtt agctaaaaaa tctgctgctt cttctggtat
    13321 ctccgaaaac agctgcacca aactctcata cgcagtatca atcaaaattt cacgtggcaa
    13381 ttggctttca ctaagccatt tttttagttg gaaagtataa aaaggacttg tctctgctgg
    13441 aagatagtct tgaatacccg ttgccagatt agaaattact tgaacagcaa atttaatgag
    13501 ccgccaacta gtttcccctg ttctgccata gcggtcaaaa cgcaaaccag tatgttctat
    13561 ttgatgttcg gacaacatat cagcccctgc aggcgttagc tttgcttcat tttcatttat
    13621 ttcgatccaa ccttgctgcg caattttttg caaaatttga taaaacttat cctgcttcaa
    13681 ttctggaaaa ctgttatgga taaacaaaag ttcgtgggta aatccataaa taagtactga
    13741 agtcgttcgt tttcccacaa ggagttggta aagagaacta atccgcagct tgtgtcctga
    13801 tgaaaatagc gataaaataa aaacaatcat attattctcc ttaagttagg atggttagaa
    13861 atgtcactac tatgtaaact tgtacctgaa cgctgtattg catgcggtct ttgtcaaatc
    13921 gaagcgccag atattttcga ttatgatgaa gaaggaatcg ttttatttgc ccaagaacca
    13981 gaggctcatc aacagttcgt gatgccggaa gaagaaacat atgtaaaaaa agcgtatcaa
    14041 aaatgtccag ttcgagcgat tttattagaa aataaaatat aaatataaac gaaactttaa
    14101 aaacgtacac taagttctct aaacttgaaa ataagccggg atctgtttct cttgttccgt
    14161 gaaatcccga cttattttga aagattcatt tgctatctat agttaattga ctagtttttt
    14221 cacggaccct ataggaccca tttatattat ttcaactttt tcagccagtt ttccaatgta
    14281 ttgacgatca cagtttgctg gtctgcgtta gaaatttctg cctctccatc tcctttttgc
    14341 tcaccatagc tgccaaaacc tccatgattt ccgccattga ttgatacata ctctgttctt
    14401 tccggtaaat actgcttagc ttcttgataa gcttcctgat tcaatacttt atcttttgtc
    14461 gccgtgatcg ataaaacagg aacagctgat tctgtcaatg ttcctttttt gtcgggataa
    14521 cttgccatga agaatacccc ttttaaatgt ttgtctgaat gattcttatt gacataacga
    14581 cttgccatta cgccacctaa tgaatggcca ccgatcacat aatcggaatt tggaaaatct
    14641 tctttgacct tatccgcttt gtttccgccc atcactgcta gatcaagtgg cattttgaca
    14701 atagcgactg aaaagccgtt ttttgctaaa tcttgcgccc aaatactata actttctggc
    14761 tctacgagag caccaggata aaaaataagt aatggagact ttccatgatt ctttttctct
    14821 ccttcaaaaa ataagtaatc ttctttgttt tttccttctt ttaccaacac ttgcgcttct
    14881 gacgatggtt cgtacgtatt ttgctgtata tagtaataac cggctccacc gagtaatcct
    14941 aaaatgatga ctaagccaat tagtattcgt ctaaatattt tcactttctc ccaccttttc
    15001 tatctgagtg accccttttt cttaatcaag tatgattata ccttaaaaaa ataagagaga
    15061 aatgcaaact ttccagcatc aaataaaaaa agacggaata tccatttgaa tatccgccct
    15121 ttttgtatgt ttatttttat ttagcacata aacgatacac agcttctgca taaatttcca
    15181 ttgctttata catactactt aattcccatc gttcattcgc ctggtgcata aagtcaggag
    15241 tatcagggaa catcgcaccg aatgccacac actggttcat cgtacgagca aaagtagcgc
    15301 cgcctgatac aaacggttca gtcatgtctc cagtaatatc gcgatatgtc cccaatagat
    15361 ttttcactaa ctcactgtct agtggcacgt ataatgaatc aaggaagtca aattcttcat
    15421 aagtcaaccc atatttttct gctgttttag ttaatttagc tactaaatca tctttcttga
    15481 atgtaacagg gattctcata tctacaccga tttttgtttg ttctggtgtg atttccaaac
    15541 ttgcaaagtt catcgttagc tcgccggatt gttcatcttc tgttttgccc acgacagctt
    15601 caccagttgc attttcttga actaattttc ctaagaaatt gattgggcca aaatcaaata
    15661 cttctgaaag agcaattgcc agacgagaaa tagcattcac tccttgtgct gcatctttcg
    15721 cgtggatact tttaccaaga acgacgatgc cttcaccttg ttcttcgaaa tcgaattcgt
    15781 gttttttcaa tgcttctttt acttcagaaa gcttttctcc agagtaagga gctgcatcag
    15841 gaacaacatt caacgctcct ccagctttta cagaaaactc gttcgttcct ggtcctgtca
    15901 agtaagcttg caataagcct ttttctgcat aaattaaagg aaattcagca tctggcgcaa
    15961 atccttgcgt aattccttct tctttttcgt tatatttttc taaacaacgc cataaatttt
    16021 cttcatctgt accaaagata aaacgaatac gtgtcttgaa ttccacgcca gcatcaatca
    16081 aagctttgac ggcatacatt gccgcaatag aaggaccttt gtcatcttgg gatccgcgac
    16141 caactaacca gccgtctttt atagttggat cgaatggttt tgtttcccaa ttattttcgt
    16201 cccctgctgg aacaacgtcc atatgacaca agataccgaa taattcatct ccagagccaa
    16261 tctctgaata accgtaatag ccttctggat ctttaaatgt tctaaatcct agcttttcac
    16321 tgatttccaa tactttatcc aaagcttcaa tcactttttt accgaaggga tgtccttgtc
    16381 ctgaatctgc ttcatctagt acggaaggaa ttcggatcaa ttcgctcaat gattctaatg
    16441 cttgttcatg atgttgttct gtcacaaatt ttttcatgat attatcctcc taatatgtta
    16501 aaaagggtca tgtcaaattc ctccgacaca acctcttctt ctcaaataat gacgttttaa
    16561 gcctgctcca tcagcaagca gaatgttctt aacaaatgat gaaagaaaaa acactcactg
    16621 aatggagcta ttgacttgtc cacgtgctta tcgatagccg tttatcggtt tcttcattct
    16681 tatgatagac cttgtacctg tatttgtcta ggtttagaac attgctgcta cacctaggat
    16741 cacacagatg gctacaaata atatcgcaat taatttaccg gtaaatttcc accacactga
    16801 aacatcgaaa cgagcaattg ctacagcacc cattacgata gcagatgttg gtgtaattaa
    16861 gtttactaaa ccagatgcag attgataagc agtgattact aaatccttac cgacgccggc
    16921 aaattcaccc ataggtccca tgattcccat tgttgcagca gctaagccgg aagttgaagg
    16981 aatcaagaat gacataggga tatagaaaat aaatgtaata atgatgaata cacttgaaga
    17041 taatgaactt aatccttgtt cgccccaatg taagatggtg tctgtgatta atccattgtt
    17101 cataaccact tggataccac gagcaacagc tactacgatt gctacaccga tcaaatcaga
    17161 agcaccagct aaaaattcag agacaaattc agactctttc atgccataag tgattgctac
    17221 tacgatggac attactaaga ataacatagt aatttctggg aagtaccagt cacctaatgg
    17281 aagcatgctt ttacctagaa ccgtacctag taccggaagg tttgttaacc agtttgtcaa
    17341 tgattcaaaa atcgtgaaat tcgcattcaa ggttgtccaa ggaatcaaac tgatgatcat
    17401 gataccaaaa gtaataaaga acaatacatt aacatgtttt tgttttttcg tgattacttc
    17461 ttgacgaccg attgcttcaa tatcgaaatg cttcatatct tctttacgtt ggttgtatac
    17521 taatgatttt gttggatctt tttcgatctt tgatgcataa cgatacacat atacaataga
    17581 aatagctaac aaaataacga acatcaagaa tcgaagacca ataccttcac ccatcgaaat
    17641 cccaacagct tgtgaagcta cgcctgttgc aaaaggattt accgttgaag ctagacagcc
    17701 tacttgagac cctactaata cgattgctac agctactact gtatcaaagc ctacagccat
    17761 catgactggg atcagtaaag catagaatgg aatcgtttct tctgccattc cgtaagtaga
    17821 gccgcctaat gcaaataaca gcatcaatac tggaataagc attttttctt ttcctttgaa
    17881 tttcttcaca actgaagcaa tcccggcatc tagtgcccct gttttgttga taatacctaa
    17941 aaatccacca acaaccaaaa tgaagaaaga gattgggatt gcagcaggtg tttctttagt
    18001 tagctccccc gctggtacac ctagcatccc attgattggc gccatgaaaa tatcccacag
    18061 cccttgagga ttttgttgta ctcgttcata tgatccagcg acgatgttgc ctgcatcgtc
    18121 tacactatag tgcccagctg gtataaacca agtaagtaca gcgattactg caataataat
    18181 aaataagacc gtatacgctg aaggcatttg aaattttttc ttttttgttt gttccatctt
    18241 cttcttcctt tcttttccgt tagagacaac aattttggct ctaatgttat tttatcaaaa
    18301 agagtatcag aacaaaaata ataacacgca ttttgtaaaa cttttgttca aaaagttcgt
    18361 ttttccctat tccctcctct tttttcagtt ttttaaagct ttcttctttt tcattgtagc
    18421 gtttcctttt ttttataaac aaacattttc gtcttttaaa aggagaagaa tttcacattt
    18481 gcaaagaatt gattgatttt tatgcataaa
//
LOCUS       DAYYCY010000041        18323 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000041 DAYYCY010000000
VERSION     DAYYCY010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.041"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(535..1038)
                     /locus_tag="ACHAZB_001141"
     CDS             complement(535..1038)
                     /locus_tag="ACHAZB_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFM8613830.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            1397..2422
                     /locus_tag="ACHAZB_001142"
     CDS             1397..2422
                     /locus_tag="ACHAZB_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFM8613831.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            2745..3623
                     /locus_tag="ACHAZB_001143"
     CDS             2745..3623
                     /locus_tag="ACHAZB_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HFM8613832.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            4099..4215
                     /locus_tag="ACHAZB_001144"
     CDS             4099..4215
                     /locus_tag="ACHAZB_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFM8613833.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            5143..5481
                     /locus_tag="ACHAZB_001145"
     CDS             5143..5481
                     /locus_tag="ACHAZB_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8613834.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            5575..5805
                     /locus_tag="ACHAZB_001146"
     CDS             5575..5805
                     /locus_tag="ACHAZB_001146"
                     /inference="COORDINATES: protein motif:HMM:NF042636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6568 family protein"
                     /protein_id="HFM8613835.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            6205..6576
                     /locus_tag="ACHAZB_001147"
     CDS             6205..6576
                     /locus_tag="ACHAZB_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HFM8613836.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(6850..7542)
                     /locus_tag="ACHAZB_001148"
     CDS             complement(6850..7542)
                     /locus_tag="ACHAZB_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8613837.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(7545..8330)
                     /locus_tag="ACHAZB_001149"
     CDS             complement(7545..8330)
                     /locus_tag="ACHAZB_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM8613838.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            8567..9967
                     /gene="lacG"
                     /locus_tag="ACHAZB_001150"
     CDS             8567..9967
                     /gene="lacG"
                     /locus_tag="ACHAZB_001150"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HFM8613839.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            9992..10309
                     /locus_tag="ACHAZB_001151"
     CDS             9992..10309
                     /locus_tag="ACHAZB_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFM8613840.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            10322..11995
                     /locus_tag="ACHAZB_001152"
     CDS             10322..11995
                     /locus_tag="ACHAZB_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HFM8613841.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            12009..13640
                     /locus_tag="ACHAZB_001153"
     CDS             12009..13640
                     /locus_tag="ACHAZB_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-beta-1,4-galactanase"
                     /protein_id="HFM8613842.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            14512..15702
                     /locus_tag="ACHAZB_001154"
     CDS             14512..15702
                     /locus_tag="ACHAZB_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISLgar5 family transposase"
                     /protein_id="HFM8613843.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHYYTPQTMSNITKSFTEEVTAFNGRELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQERG
                     LKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKEVALEARGTFAEKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLSQ
                     LIEN"
     gene            15836..18025
                     /locus_tag="ACHAZB_001155"
     CDS             15836..18025
                     /locus_tag="ACHAZB_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8613844.1"
                     /translation="MFISTLQIRNFRNYESEIFRFDNETNTIIGENDSGKTNALTALR
                     ILLDDSYYYSSKTLKESDFFHGIQNGWQGHWIIISATFEGISEEEFDNEICASISLNS
                     ESQTILEELISNADKGVGSISLFIRPNKAIRKQLFDISTELDSHQFNEFRNSIRLSDY
                     EFYYTSKSNLNFCINENYDKLVGRLNDNIASNPEEDDEALLGSRIDMSDIFKHISVVY
                     VDALRDVLREMKNNRNPVKRIMETIESKISSDNVESLKTIIQQLNETITSVPEIRKIG
                     ENINRQLNSIIGSVYSPNLLLSSTMSDDMGSLAKFLSMKPEQNIDLDLLGLGHLNMIY
                     LALKIVEFEACRSRELLNIMLIEEPEAHIHHHIQKTLFEGLNLQKNYTQILMTTHSVH
                     LAEVSEISRMNVLKSFNGKSIVMNPANNLNSFAKEKLKKNNLNLINAVERYLDVKRNG
                     LLFSKGVILVEGDAEEILIPQIVKKLFGINLDELGIGLINVGSTSFEYIASLFDDDRI
                     QRKCAIVTDLDEQAIPSEHRLYKSTAQEKGQARKEKLSSLFNENNWVNCFYANTTFEI
                     EFLDVNKENILTYVSPIIDDAFKDKKTITAYKEEIQSEQYLERNLAMLKLANHVGKGW
                     FAIELGEKIDFLIKIPKYILNAIAFACQEIVDLSIYKKILLHTIDLYEETTELSALSA
                     SLKTSDTADLNIAIISLLEYFGKDEDVVYLINQIENYTKILYAEVSK"
     gene            18022..>18323
                     /locus_tag="ACHAZB_001156"
     CDS             18022..>18323
                     /locus_tag="ACHAZB_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFM8613845.1"
                     /translation="MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKI
                     AYQAIKHDGSLKRIIAITYTNRAAEEIKERLSLLSIDDDINIWVGTIHQFCLEFII"
BASE COUNT         5983 a         2950 c         3652 g         5738 t
ORIGIN      
        1 ggaataaaaa aataacatat ctcattttac gcttttgtta ttttatactt aaaaaataac
       61 gacacagaaa ataccgtgtc tcagatttct ctactacttc taaaaactac tgtttttttc
      121 ctttgaaatg ccttcaaaaa gtaggatcag gcagaatata ctggcagaat caaggagaag
      181 ggagcctaaa taaggagaaa aaccatagaa aatataaagt aattacttat aaaacaagct
      241 ttttatagaa aaataatata cctgagtagg gtataaatta agacggaatt cctttctaga
      301 ttgagatatt gactaggaag aatgttaaga ttttaagcct ttaagctaaa aaaaaaccct
      361 ttttgttaaa taaggggaat atagcctgtc agtttcaaac tgcaagtcgt cctgctgcaa
      421 taatatagta gattagagat gaaaatagat ttttttaaac caatgactga ataaaaagac
      481 cgtcatagat acacattgga atgtgtctat tacggtcttt ttttacgaac gattttattg
      541 aagacggaca gttcgggtca aaaataaaaa agctacgatc agaaataaga ataaaatgta
      601 tcccaaaata atggctagac ggtctaactt atttgtcgat agatgtttgt ttagaagttg
      661 ataggcccat aaacctatca gtcctcctgt tgtattagtt atcaaatcag tgatgtccgc
      721 tacgccaata ccgaatagga attgaatcaa ttctgccaag aagcttacaa ggaaaataat
      781 ccctaatttt ttagaaaaag aaagtctctt gaaattgaca ttcaaaagca ccccaaaagg
      841 aaaaaagaca atcaagttat aagtggtctc acgtaatacc gtcgtatttt caaaagaaaa
      901 agtagaaaaa gggacgagat tgacattgct gtaagaataa gtgagaaatt gtggaagatt
      961 aaatgatagc ttgaataaaa tcaaccataa tagaaagaat aaataaatcc ctaataaaaa
     1021 ttttgataca taccgcatag acatctcctc catttaaaaa gtataccatc gaaaaccgct
     1081 gaaaaatcat aaaaaagtat gaagtttaag gaccaacgta gttatcgtac gaaaattaga
     1141 agcttttttt tgttgggaat ccggtttctt taacaaagct aagatagcga gttgcttgga
     1201 aaagttgcta tcttttttct gtcaaaacga ctcggaatgt tcgttttttg ttctaatttc
     1261 tccaagttgt ttactttttg atactaagtt tatgaaaaat caagaaatag ttgttatact
     1321 aggtaatgct gacaaacggg attggcattt catcttggtt cagctttttt tccaattact
     1381 aggaaggagg gatagattgt tcaagactta ttttcgtttc aataaaatac aagataatcc
     1441 ttttcgtatc atgaatctga tgctacttat ctttattgta ttatggttag gttacataaa
     1501 tcatgatttt ttgatttcaa gtttttcatc attagggatc tttacttttt tctattatca
     1561 aaacataccg atgaaatatt tgctgaaacg tatgtttctg atcggttgcg gactgttggt
     1621 tgctttcgtt ttggggatct tatcgactta tgttttatgg cttgaaccat ttgccgtagc
     1681 tttggttgct ttttccagca gattcgtgtt gcgattattc catatttcta aaccaggtgg
     1741 cttgtttttt gcgatgcttt ctgccatggg aacgagcatg cagctgccaa tcgctcaatt
     1801 accgatcgtc tcactttatt tttttatggg tgtcgttttt gccctgattg cggcagtgat
     1861 tacaaaatta ctggattcac ggcctgagca gacaattgaa aaagcaactt taaaagaaag
     1921 atttcacgaa gagcctttag tgatcattga ttctgttttc tacagcgctg ccttatttct
     1981 ttccgtttat gtcagtcacg gcttgaatct gcataatcct tattggttga ccttgtcatg
     2041 tgcatcgatt ttgctagcag aaaacttgga tgcgatgaag catcgacaag tccaatatct
     2101 gattggatcg atgggtgggt tatgtgtgtc ggcattcttg tcatttgtgc cgtttacgca
     2161 gttgcagacc atttttctga tcacttttct atatggaatc gcacaatttc tagttgctag
     2221 aaattatgca gttgctaata tttttcttaa tccgatggcg ttgatgctat ctacacttat
     2281 tcgtggagca tacttgattt ctttgatcga atatcgtttt ttagggatcg tcataggaag
     2341 tttcatcggt ttaggtgttg cgtgggtaat gacggttggt ttgcaacatt atttaacggt
     2401 tgttcgtcaa aaattagaat agaaggacaa atatatgtgc caaaagaggt tatgacaaga
     2461 tttttgtcac aacctctaat tcaaggtgtt caaaagccag attctcagca ttaagtcaaa
     2521 ttcacacaaa aatgtggaaa ggaactttca aaaaaatttt atatttattc tatcaaaaaa
     2581 ttttgtttat gatggtaact gaataaactt tcctgagatg gtcaatgctt tccttgaaag
     2641 tcttacagat attctttctt catagaaagt ttaagtttta tagttagtgt acaataaaag
     2701 tatgtttata ccatatggtg tccggataat cagtcattta aacaatgagg ttcaaagata
     2761 tgaaaagggg aaatttgatg aagaaaataa gcaatgaaat ccaaattctg gcagaaactt
     2821 taactcttcc caaagtcgtt atagagcaaa tgattgtaca agcaaaaaac atccttgatg
     2881 aggtgtttga gcaatatagt catgaactaa tggatcctaa taaagcaaag gcgactgcaa
     2941 agaaaataga caaagtctat gaagaggaac cttttgcagc attagctatt tatctatatg
     3001 cagcttctca aagttggaaa aaaatttatc aaccattgaa gattccaaga actatctatt
     3061 tggcaacaat gaatgctttt actagattta ttcatgaaca tttccaagta acaggaagct
     3121 atcgctttga ccgtggtttt tggatttggc attacactag tggcttgatc tttcggattg
     3181 gtgaattgga gtacgaaatg acatattttc cgaaagggta cacggatcta agattagaag
     3241 gaaagcagtg cctttctctt catattcctt ccgatgcaga tttatctcag aataagataa
     3301 gagatagtta cgaaagtgcc attcgttttt ttgaacttta ttttcctgat taccattacc
     3361 aagtgatgta cacagatact tggttactat cacccaatct tactaaatgg ttgaaaaaag
     3421 agtcaaaaat ctgtcttttt gcagccgatt atcgattatt atcagttgat gaacaggacg
     3481 atagtggagt cccttggatc tttggacgag tagatgccca aatacatgat tatccagaaa
     3541 gtacgagttt acagcgacaa gcaaaagagc aattgcttgc aggcgagcac attggctctg
     3601 gcttaggtat tcttacttca taagtttagt acttcgaaat agtttaaaag agcgtgagac
     3661 aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcgg aagcagaagc
     3721 aactccttcg gaaataagct gaaattcaca aaaatttgaa aagcaatttt cgtgaatttc
     3781 ttcttatttc ttggagttaa acgcttctgt cccagcctct tttttagtgt tctatcgaac
     3841 acacagtacg ttcaataggt aaactagcta taacaataga tattaaagtt aacggacaag
     3901 agggcctttt ttaggtagct aaaacgtaat aatgaaaatt tatttccaga gataggagtc
     3961 aatcagctag tctgatcgac tgttttaaca agttgacttt cattttaact tttgatacca
     4021 tttaaaacac aaatcgtaat tgtttcgatt tgtgtttttt agcatgacta caatcaaaga
     4081 agaaagtggg tcgtccatat ggaaaaaaca gatatctcaa gtgcataccg ccgcttgaaa
     4141 agcccaaata tcaaaacacg aaaacgtgcg ctgaagatca tcaaagatgt gaaacggaat
     4201 agtgggaaaa ggtaagaaga aggaaactta attaaaaaaa tagccgaaaa acgtttttcg
     4261 gctattttca ataatcggtt tttgtgtaag gtttccgaaa gaactgctgt tgattttcca
     4321 tttttaacat atgataaatt ggtagaatct ttcttttcaa attgatacca ttccctaatt
     4381 ccagttatat agttaactac agaaccacaa agcacgtacg tttataaaag tattatctgt
     4441 agttattttt caattccctt tcaacctctt attttgacca gtagtattga tcactttacc
     4501 ttttaacacc tttgttcgtt tttgaaacat ttttagtcaa gaatggaaac gtaactacat
     4561 aatcaaaggt tttgcgaaat atttcatgaa atatttcatg tagaagcgag caatacggtt
     4621 gcaaataacc attatcgttt aataagcagt tacgaaagct cccaatatct ctaacgttca
     4681 gctcactcca tttcttttct tcaaaaaaat cagtgattcg tatctctttc tatttgtcga
     4741 aaaagttaac tttaaaaagt aacgccgaaa aaatatgcaa aaaaataaaa aagcagttac
     4801 tattgctaga ataacatcaa ggaataataa gttttggttt tcagataata gtcgataagt
     4861 tgtgtattgg aattttagat acagtaataa agtatttttc atgaaatttt gtgagaatca
     4921 ctattacaag tatctttaat aaaattaatg gttaaggagg aacttaaagt agaatgtaaa
     4981 tgaatagttg tagtcagtcg attgtgatac tagactcaaa gttgcaggaa taagtttttc
     5041 aatttgtgat tggaagataa aaaatgaaaa agaagggctt ttttttatac tttgttgatt
     5101 gtagtgttta ttttccctct gtttagccaa gtaacatttt ctatgactca accagtttca
     5161 agtgaggaac atgataacct ttttcgtgct acagaagagt ttaagaaata tcaggaaaat
     5221 attgttccgg agactccatt agctagtgag ccaattactg atattgatgg aaaccaaata
     5281 ggatggtata tacagtacga agttatggtg gtaaataatg actcaatagt aaaaaataca
     5341 gtaggcttag agctaacacc gattgaaaag gtgagttcat tgttaacgta tatttatact
     5401 gatgaaagtg acgagactga gatcatttta acggattact caaaaattgc tactgataaa
     5461 aagtatatat tgtggatgta gacactagtg aaatacttta tgatatagaa attgatgaaa
     5521 atttactaga tgtagcacaa gaaatcgatc atcaaaagca ggaagctatt cgatatgctg
     5581 gtagaaaagg gaaaagaaaa tatttattac gtgaatgtag caaaagttag agaagatgaa
     5641 aatgagtgga aagaattcaa gagtcgctac tcaattaact cgactccgac atttacagtt
     5701 tacagagaag gatcgataga aaaaactgtt ttttggacta aagaaagtgg aatgtcttta
     5761 gctgaagtag aagagttttt ggattatgtt agtatgcaac agtaagggag ttcttattat
     5821 gaaaaatttt gacacatatt atgttattgt tttttcgata tctttgctgt taatattact
     5881 aactttaatg cagtataaag agtatccaga gtattttatt ttaacttttg tatatttagt
     5941 aagtagctac acacttctat ataaattcag aagttcttct agagtttcta aagtttgttt
     6001 cttaattagc ttattgattc tataatattt ttgatttata taggaaatta aaaatattat
     6061 agaatcaaca ctcagagaac gggttggttt ttaatagatt taataagtac tgataatgaa
     6121 atttctcctg gttgggaaaa cgaagttggt taccctgtag aagaattttt tcaagattac
     6181 tttgcggagt atgccgatag gataatgaga gattacgtaa cagataaaac tcaagaagtc
     6241 atcaatcaat cccagtcatt aaatgaatta tctgataagg cgaaagaaga agcagttgcc
     6301 attatttctt cagggattag tgactatatt gatatgagtg gtgaaatcaa ttatggcgat
     6361 aaagacaaaa ttgtacgcat gataaaagct tttatcaaaa cttttgatgt gaaggcagat
     6421 cttaagttta atgatactta cctagtagga aaattaatta atatccttgg gacagagaaa
     6481 gatacggaaa cgtttatttc ctctttgtct atgttgttta cagataagca tttttcaact
     6541 cagcttggtg gagaggaact agtggaaatc ttttaaccat tacgcccagc attaataaaa
     6601 gtatatcaaa aaagtactcc tgatgatttt taagattggt ttgagaaata atgtgaaaac
     6661 actattttta atgttaatta tagatagaga tgaacgcgtc atatggttaa aagaatgaaa
     6721 aagtcaaaga tacactgcta tatttcacta aggatatagc agtgtatctt tcatgtcgtt
     6781 tcactggcag ttttttataa aaggaggtgt gcatttcagt tgcttttctt tggtagttag
     6841 ttgaatcgtt taagaattaa cggatgtggc agacaggtgc tctgtccaaa aaccagtaaa
     6901 atctttgatg gttccatctt tatttagctg tgcctgttca aatactgttt tgtttttacc
     6961 gaatggatca attgcaataa ttgttccgat ccccgcacgt ttggcagcaa gtaatcctga
     7021 gtaagcatct tcgataacaa gacaatcagc cggagctaag ctcagtttgt ctgccgcttt
     7081 aagaaaaata tccggttgcg gtttcccagg gaaagagccg tcgtcataga caatcttgtc
     7141 ccagtcaaac cagcgggcta aatcgaaaat atcaaaataa aaagcaacat tctctttgac
     7201 agtagccgtg gcaatcgtta atggaatctg ttggtcagtc agctggtcaa gagtcgcttc
     7261 aagccctttt gtcaactgaa gctcgttttc attttgcaag caaagcatac gataatagct
     7321 ttctttttct tctgacaata cggcgatctc agaggcggtc aaatgctctg aaatgaaatg
     7381 tttcaaaatc tccgtattcg tccgaccatg aatatttttt aaaatatcct cgtctgataa
     7441 accatctttt gtatgatcat gtatcatatg cagccaagca gcttcatgca gatgagagtc
     7501 caaaaacatt gttccgttga aatcaaatat aatccctttc atagttacgc tcctatagtg
     7561 gtattttcgg ttgaatcagc aatttgcaga tcgacatatt gtgcatagtg ctcttgaacc
     7621 tcccgcgaga tctggcgatc cgtaatgagc agatcgaaat catgcaaatt ataaaacaca
     7681 taaaaatcat ctcgattgaa ttttttatta tcaacgagta gatatttctt acgggaatta
     7741 tttagagcga tctgctgtgc ttctccttct tcggtactgt aagtagagat cgcttcgttg
     7801 tgaacgccat ttgaactgat aaatgctttt gtaaacttta gtcttcttaa attgtcgttg
     7861 gtgatcggtc cgacaaatgc gccggtattt ttccggaaat caccgccaat cagtagaatt
     7921 tctggtccat ctgcttgagc aagaatatca aaaactggat aattatttgt aatgatccga
     7981 attttcttgt tcagtaaatg cgtcgccaat agttcgattg ttgtacctgg tcctaagaaa
     8041 atcgtttctc cctcgtgaat caaggaggct gctgtggcag caatttcttt tttttcttga
     8101 atctgcagag tggctttttc agtgtgagaa agttcttgat ctaaagtgta ggaaagactt
     8161 tgagcccctc catggacacg cagcagcttg cccgattttt ctaattcatc taaatctctg
     8221 cgaacggtca tatcagaaac atctaattga ttgatgattt cattaacggt aaggattcct
     8281 tttttattga ccatttctac gatagtaagc aatctttctt ttttcaacat tgtcttcacc
     8341 tctaatttaa ggataatcta tcatataaac ggacaacagt caatctaatt cgaacaaaag
     8401 taaacattta gggtggtttt tatgtttgtt acaatagatt ttattgctgt tttctggttt
     8461 ttatgattat ttgtgtttgt ttttgttgac aaatgttttg gatgcgttta caatgcaatc
     8521 agttagaaag agcgaaaact gattatagat ttgaggagag atgtacatgc ttaaattacc
     8581 agaagatttt atttttggcg gagccacagc agcctatcag gttgaaggag ccactaagga
     8641 aggtggcaaa ggcgctgttg cttgggatga ctttttagag gaacaagggc gattcagtcc
     8701 cgatccggca agtgattttt atcatcaata tgcaaaggac attgaattgt gtgaacgttt
     8761 tggtgtaaat ggtctaagat tatccattgc ttggagccgc atttttcctg atggagctgg
     8821 gaaaccaaat ccagaaggaa tcgcttttta tcatcgtgtg ttcgaagaat gtaagaaaag
     8881 aaacgtaacg ccgtttgtga cgttgcatca ttttgatacc cctaaacgtt tgtttgacca
     8941 tggagatttt ttgaatcgag aaacaataga agcatttgtt tcctatgcca ttttttgttt
     9001 tcatgaattc aaagaagtca aggtgtggag tacctttaat gaaatctatc cagttgcaac
     9061 caatcaatat ttgttaggtg ttttcccacc aggaatcaaa tatgatttta caaagatcgt
     9121 cgcctgtctt cataatatga tggttgctca tgcccgtgta gtgaactatt tcaaagaaaa
     9181 cgaacttccg ggtgaaattg gtgtagttca ctcattagag acgaaatatg cggcaacaga
     9241 cgcgccggaa gataagcatg ctgccttttt agacgatgca ttgtcgatcc gtttcttatt
     9301 agatgctacc tatttaggct attattcaac tgaaaccttg actgctcttg atgagatatg
     9361 tgaagcaaat caggccagct atcattttcc tgaagaagat ttcgttgaat tgaaaaaagc
     9421 atccacgcgt aatgactact tagggatcaa tcactaccaa tgccattttg tcaaagctta
     9481 cgatggagaa aatgcaatcc atcataacgg tacgggtgaa aaagggacct ctgtttataa
     9541 agtgaaggga atcggagaac gtatttataa agaaggaatt cctcgaactg attgggactg
     9601 gctgatttat ccagaaggtc tatatgactt gttgctgcga attaaaagcg attacccaca
     9661 ttacaacaaa atctatatca ctgagaacgg gatggggtat aaagatcagt ttgaagatgg
     9721 cattattatg gatcagccga gaatcgacta tttgagagtc tatttggaat ccttaagcaa
     9781 ggcgataaca gccggggtaa atgtgaaggg gtattttttg tggtcgctta tggatctttt
     9841 ctcatggaca aatgggtata acaaaaggta tggcttgttt tatgttgatt ttgaaaccca
     9901 gaagcgttac ccaaaagaat cagcttactg gtacaagttg gtcagtgaaa ccaaaacgat
     9961 tatttaataa gtgataatgg aggaagaaaa gatgaataaa gaggaattac aaatgcttgg
    10021 ttttgagatc gttgcatatt caggcgacgc tcgcagtaca ttattgaaat tattgaaaga
    10081 agtaagacaa ggaaactttg aacatgttga ttcggcgttg aaagaagccg acgaaaattt
    10141 aacccttgca cataattcgc aaacaaaaat tttagcggaa gaagcatctg ggaaagagat
    10201 ggagatggga ttcattttta tccatgggca ggatcattta atgaccactt tattgctgag
    10261 agatctgatt caagacttta ttgtactgta cagacagaat caaggatagg agaagaacgc
    10321 tatggatgga atcattaaac aaatcgaaaa agggaaacca ttttttgaaa aaatctcacg
    10381 taatagttat ttaggagccg ttcgtgacgg atttttaaca gcgatgccag ctattttatt
    10441 ttcaagcatt ttcattttga ttgctgcggt tcctgagatt tttggttacc aatggcctga
    10501 atctgtctcc acatggctat ggaaagtcta taattattcg atgggtgttg tagggttact
    10561 cgtttcagca accactgctc gctgcttggc tgattcgatg aatcgtcgct taccgaaaaa
    10621 caaagtaatc aacaccacct ctgtgatgct ggcctctgtg gtaagtttcc tgttgttaag
    10681 cgtctctccg attgaaggtg gattttcaac ggaatatatg gggacaaagg gactactggc
    10741 ttcctttgtc gctgcatttt taacagttaa tttatataag ttttgtgtgc tgaaagatat
    10801 taccattaaa atgcctaaag aagttcctgg aacgatttcg caaaccttcc gagatatttt
    10861 tcctttctct ttttcagtat ttgcggccgt tttaattgat ttaggcattc gacaaatctt
    10921 ccaaatctca tttgcagaag caatcattac ggtgctgcag ccgctattta cagcagctga
    10981 cgggtactta ggaatcgcta ttatctgggg agcgatggcg ctgttttggt ttgtcggtgt
    11041 tcatggaccg tcgatcgtgg agccggcaat tgcggcaatt atctatgcca atgtggaaac
    11101 caatctgcaa ttgttcaatt ccggtgaaca agcttctaaa gtactgacgg tgggtttagg
    11161 gaattttgta ggaactatgg gaggaactgg ggcaacattg gttgttcctt ttatcttcct
    11221 actctttgct aagtctaagc agttgaaggc agttgggaaa gcttccttta ttccagtaag
    11281 tttcgctgtc aacgaaccat tgctatttgc ggcacccatg attttgaacc cttatttttt
    11341 tattccattt ttgttaacac ctgttgtaaa cgtttgcctg ttcaagtttt ttgtcgatgt
    11401 attgggcatg aacagcttta tgtacgtttt gccttgggca actccagcac cgatcggttt
    11461 ggttctagga acgggcatgg gtatcttagc ttttgttcta gcagcggttc taatcgtcgt
    11521 tgacttcctg atctatctac cattttgcaa agcatacgat gctgtcttag tagagaaaga
    11581 agcacaaaaa gcagaggtga tcgcaaccga ggaaggtgct tctgccgatg tacgtgagga
    11641 agtacctgtg gcagcgagtc aagttatcag cgatgcaaat aaagaaatca atgtgttggt
    11701 actttgtgct ggcgcaggga caagtgcgat gttagctaat gctttaaaaa aaggtgcatc
    11761 tgaatcgaaa gcaccgatca gtgcaatggc gggtgcttat ggttctcatt acgaaatgct
    11821 gcaggatttt gatatggtgg tcttagcacc gcaagtcaat tcttactatg atgatatcaa
    11881 agaagacacc gatcgcttag gaatcaagct ggttgcgaca aaaggagcag aatatatcgg
    11941 attaacgaga gatcctgaaa aagctgttca gtttattctg aaccaatttc aataaaggag
    12001 gagccataat gaaaatggga atttggttgc gtaaagctgt tttatttgca gctgtgttag
    12061 gggctttggg ggctgtttca aaaactgttg aagcatctgc cagcgaattt attcgcggag
    12121 cagatatttc gatcctgtca gatatggaaa aatctggtgc aaaatattat gaagacgata
    12181 tttcaaaaga tgccttagag attttaaaaa ataatggtgt caactatgtt cgactgcgtt
    12241 tgtggcagga tccttataat agtaatggag aaagttatgg agcagggaca aatgatctgg
    12301 aaacgacgat tgaccttgca aaacgagcaa aaaatctggg acttaaggtg ctgctggatt
    12361 ttcactacag tgatttttgg gttgatcctg ggaaacagaa tcttcctaaa gcatggcaag
    12421 gtctgacctt tgaagaaatg aatacggcat tgtacgacta tacaaagaac gttctgtcgg
    12481 aaatgaagca gctggacgtt tatcctgata tggttcagat cggcaatgaa ctgaatagcg
    12541 gaatgctctg gccttatggc aagtcttggg gagaaggcgg gggagaattt gatcgattgg
    12601 ctgctttttt gaagtctggt atccaagctg ttcgagacac tcaacctaaa acaactccag
    12661 taatgctcca cttagctgat ggtggcgata caggagcatt tacttggtgg tttgatgaaa
    12721 tcacgagtcg aggtgtgtca tttgatttaa ttggagtgtc gtattatccg tattggcacg
    12781 gttcaatgaa tgaacttcaa accaatatga atgcgatcag ccaacggtat ggtaaagaag
    12841 tgatcgtagt tgaaacagct tatgggcata caacagcgaa tgccgatact atgccaaatg
    12901 cttttggtga agccgaagca gctgcggggg gatatgaacc aacaccgaca ggacaagcag
    12961 agtatttgct ggatcttgcc gatcggattc aggcagtacc gaataatcgc ggggcgggct
    13021 tcttctattg ggaacctctt tggtacaacg gaaatgtgag ttgggcaaca caagcaggta
    13081 tgaattatct cagtgttcaa tccactatgg gaaacgaatg ggacaaccaa gcgatgtttg
    13141 attttgctgg aaacgccttg ccaagcttgc gtgcgtttaa acaagcaggg gcgcaaataa
    13201 atctagttaa aaatgcaagt ttcgaaagcg atggaccaac gaccagtcct tccaattggc
    13261 aaacctggtt tcaacaagga accgctaagg gtacgatcaa aacggaagcg ggcacttttt
    13321 ttggggacta caaactaact ttttggcagg atcaagcata cgaggcatca gcttatcaaa
    13381 atgttagtgg cataagcaaa ggaacttaca cattgagtgc ctatgtaatg tctgggggag
    13441 agtttgatac taatcagtta tatatcaaaa attatggtgg agcggaacta aatactgcgg
    13501 ttgttaaaac aagtgaatgg acaaaaatta caatcaaaga cattccaatt acaacaggtg
    13561 tttgtgaaat tggggtttat agcaaggctt caggaaatgc ttggacgaac gttgattttg
    13621 tttcattggt gaaacaatag actgcggatc gtttcgattc aagatcggag gttcccttca
    13681 gccacatcgt tagcgtagaa ggcaggaatc gaacctgcac ctcgcctttg ccaaaaataa
    13741 ccagaaacga tttccagtca tttttaccaa aacgagtaaa gcaccagact tcatctacac
    13801 tttgtattat gctaaagaga gttcaacgat ttcgtaagaa gcaatgaaaa aattgcaaaa
    13861 agttgtgcag ataacataaa gtttgtttaa gaaagggtaa ccttagaatt ccgtcgtgaa
    13921 tatgatttga gcacttagat ggatagaaaa aagttctcgg aaaacagttc gtttttcgag
    13981 aacttctttt tatgataaga atgaaacaaa ttaacttata ttatagtctc ttttggtagg
    14041 gaaccatctt atttttaatg aaaatcaagc attttagcta ttgaagaatg ttgatttata
    14101 gggattcccc ctccatatta tgttggctct ttattcccac tctgttttca acgtaaagtg
    14161 tataaaacag aatgcgatac tatcaaggga gttaaaacaa tatacagaac agtaaaacac
    14221 tataaataaa aataaaagtg caataaaagt gcaatttttg cacctaatca tactctgttt
    14281 atatatgaaa actagattct gaatccaata tttggttaac gagaggcgtt cgaatttgaa
    14341 cgtcttttat atattacttt caaataaata tgaggtagaa tactatctat tttcttttta
    14401 acccaattgt tataatggag agtgtaaaat attttgtgta aatagaaaaa aggaagtccc
    14461 ttctgtagaa tagagttacc acaacacatt cacagaagag aggacttccc tatgaacgat
    14521 tttactacag aaattctaaa gactctagcg aacaaaggcg atttgaatga attattccgt
    14581 gtccatttgg agaaagcagt caatacgctt ctcaaaacgg agttaacggc tttcctagat
    14641 tacgaaaagt atgatcgcat tggttttaac acgggtaatt ctcgtaacgg ctcctatgac
    14701 cgtacggtca agaccgagta tggggaactt catctccaga ttccgcgcga ccgcaatggt
    14761 gagttcaagc aacagactgt tcctgcttat agacggacga atgacacgtt agaggagacc
    14821 gtcattcacc tcttccgaaa aggtattacc atgtcggaaa tcgcagactt gattgagaaa
    14881 atgtatgggc attactacac gccccaaacc atgtccaata taacaaaatc atttacagaa
    14941 gaggtaacgg cgtttaatgg gcgggagctt catgaccgtt atgctgctat ttatatggac
    15001 gcaacgtata ttccgttaaa gcggaaaacc gtcgccaagg aagctattca tatcgcagtt
    15061 ggcattcgcc cggacggatc aaaggaagta ttgagctatg cgattgcacc gactgaatcc
    15121 atcacgattt gggaggaaat tttattggac cttcaagagc gcggtttgaa aaatgtcctc
    15181 ctgttcatca cggatggctt aaaggggatg gtaggagcga ttagtcggtt ctatcccaaa
    15241 gctcgttttc aacattgttg tgtacacgtt tcccgtaata tcagtcataa agtgcgtgtc
    15301 gatgatcgta aggaagtctg tgatgatttt aaaatggtgt atcaagcctc atctaaagag
    15361 gtggcgttgg aagcacgtgg tacttttgcg gaaaaatgga aaaccagcta tccaaaagtg
    15421 gttgaatcga ttctttcgaa cgatcacttg ctcactttct atgatttccc cttggccata
    15481 cgcaagagta tttattctac gaacttgatt gaatccttta ataaacaaat caagaaatac
    15541 agccaccgca aggaacagtt ccaaaatgaa gagtcgatgg aacgtttctt agtctcgtct
    15601 tttgatactt acaaccaaaa attcctaggt cgcagtcata aaggtttcca acaggccgaa
    15661 ggcgagctcg aacaaatgct aagccaactg attgagaatt agaaacaatc tgcagtaggg
    15721 aagaatcatt tacacaaaat tattgacgct cccttataat gagaactttt actaacataa
    15781 tttatgataa acttactata taatcatatt aattaaatag ttaggagatc tgcttatgtt
    15841 tatatctact cttcaaattc gaaattttag aaattatgaa tccgaaatct ttcgattcga
    15901 taatgagaca aatactatta taggtgagaa tgattcaggg aaaaccaatg ctcttactgc
    15961 attgagaatc ttattagatg atagttatta ctatagtagt aaaacactaa aagaatcaga
    16021 tttttttcat ggaattcaaa atgggtggca agggcattgg attattatct ctgcgacatt
    16081 tgaaggaatc agcgaagaag aatttgacaa tgagatttgt gcttccattt ctttaaattc
    16141 tgaaagtcaa acaatcttag aagaattgat ttcaaatgca gataaaggtg taggtagtat
    16201 ttctctattt attagaccta acaaagctat tagaaagcaa ttatttgata tttccactga
    16261 attagactct catcaattta acgaatttag aaattctatt cgtctatccg actatgaatt
    16321 ttactacaca tctaaatcaa atctaaattt ctgtatcaat gaaaattatg ataaattggt
    16381 aggaagactc aatgacaata tagctagtaa tcctgaagaa gacgatgaag ctctgttggg
    16441 ctcaagaatt gacatgtcag atattttcaa gcatatttcg gttgtttatg ttgacgctct
    16501 tcgagatgtt cttagagaaa tgaaaaacaa tcgaaaccca gtcaaaagaa tcatggaaac
    16561 aattgaatca aaaatatcat cagataatgt tgagtcatta aaaacaatta tacagcaatt
    16621 aaatgagact attaccagtg ttcctgaaat taggaaaatt ggcgaaaata ttaatcggca
    16681 actaaatagc ataattggtt cagtgtattc tcctaatctt cttctttcct caactatgtc
    16741 agatgatatg ggttctttag caaaatttct ttcaatgaag ccagaacaaa atatcgactt
    16801 agacttacta gggttaggac atctaaatat gatttattta gctttgaaaa tagtcgaatt
    16861 tgaagcgtgt cgttcaagag aactacttaa tattatgttg attgaagaac cagaagctca
    16921 tattcatcat catattcaaa aaactctgtt cgaaggatta aatcttcaaa aaaactatac
    16981 tcagattctc atgaccacac attctgtaca tttagctgaa gtgtctgaaa tatctcggat
    17041 gaatgtatta aaatcgttta atggcaaatc tatcgttatg aatccagcaa ataatctgaa
    17101 ctcatttgct aaagaaaagt taaaaaagaa caatctaaat ctaataaacg cagtagagag
    17161 atatttagat gttaaaagaa atggattgct tttttctaaa ggtgttatct tagttgaagg
    17221 agatgctgaa gaaatactta ttcctcaaat agtaaaaaaa ttatttggta taaacttaga
    17281 cgaacttgga ataggtttga ttaatgttgg tagtacttcg tttgaatata tcgctagctt
    17341 gtttgacgat gatagaatac agagaaagtg tgctattgta actgatttag atgaacaggc
    17401 aatcccatca gaacatcgat tgtataaaag cactgcacaa gagaaaggtc aagcaagaaa
    17461 agaaaaatta tcctcccttt ttaatgaaaa caattgggtt aattgttttt atgctaatac
    17521 aacatttgaa attgagtttt tagatgtgaa taaagaaaat attctaactt atgtcagtcc
    17581 aattatagat gacgcattca aagataaaaa aacaataact gcttataaag aagaaataca
    17641 aagtgaacaa tatttagaaa gaaatttagc gatgttaaaa ctagccaatc atgttggtaa
    17701 ggggtggttt gcaattgagt taggtgaaaa aattgacttt ttaatcaaaa ttcctaagta
    17761 tattcttaat gctattgctt ttgcatgtca ggaaatagta gatctctcaa tttataaaaa
    17821 aattctactc catacaatcg acttgtacga ggaaactaca gaattgtcag ctttatcagc
    17881 tagtttaaaa acaagtgata cagctgattt gaatatagca ataatttctt tattagagta
    17941 ctttggtaaa gatgaggatg ttgtgtatct aatcaatcag atagaaaatt acacaaaaat
    18001 actttatgct gaggtttcaa aatgacaaat atcttcgaaa aaatcgtaaa tgatttaaat
    18061 ttagaacaaa aagatgttgt ggaaagtgaa gataaccttt atgtaaatgc atgtcctggg
    18121 agtgggaaaa caagggtact tacaaggaaa attgcttacc aagcaattaa acacgatgga
    18181 tcactaaaaa gaataattgc tatcacatac actaatagag ctgctgaaga aattaaggaa
    18241 cgcctaagtt tacttagtat agatgatgat attaatattt gggtggggac aatacatcag
    18301 ttttgtcttg aatttattat tta
//
LOCUS       DAYYCY010000042        18100 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000042 DAYYCY010000000
VERSION     DAYYCY010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18100
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.042"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1071
                     /locus_tag="ACHAZB_001157"
     CDS             <1..1071
                     /locus_tag="ACHAZB_001157"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HFM8613846.1"
                     /translation="VFKIISVQTGMVDKPNERRINKVPMPSAGGLPIFISFVISTLFL
                     FRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPKQKSIGILIASLIIYFVADIRIDS
                     FTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDGLDGLAAGISLIGLITIGIIGYFF
                     LHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLGDTGALFLGFMMAVLSLQGLKNVT
                     FVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTADKKHLHHRLISLGFTHKGAVLTI
                     YAMALMFSFTAMVMNYTGRIGTIVLIIAMLFAAILLFELIGLINEKHQFILKTLRFLG
                     NKEYRTQVKQKYLKKWNRHIDE"
     gene            1064..1864
                     /locus_tag="ACHAZB_001158"
     CDS             1064..1864
                     /locus_tag="ACHAZB_001158"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM8613847.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            1864..2691
                     /locus_tag="ACHAZB_001159"
     CDS             1864..2691
                     /locus_tag="ACHAZB_001159"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM8613848.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            2696..3403
                     /locus_tag="ACHAZB_001160"
     CDS             2696..3403
                     /locus_tag="ACHAZB_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM8613849.1"
                     /translation="MRSVCIATFNGGRYLREQLDSILSQLSAEDEVVISDDGSVDDTW
                     DILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWLETKVKKITD
                     YFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYIGAGMAFRSS
                     LKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIETHSKLRQKIS
                     WRVHLITALIKRKWFKR"
     gene            3503..4369
                     /gene="rfbA"
                     /locus_tag="ACHAZB_001161"
     CDS             3503..4369
                     /gene="rfbA"
                     /locus_tag="ACHAZB_001161"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFM8613850.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            4383..4955
                     /gene="rfbC"
                     /locus_tag="ACHAZB_001162"
     CDS             4383..4955
                     /gene="rfbC"
                     /locus_tag="ACHAZB_001162"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFM8613851.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            4977..6005
                     /gene="rfbB"
                     /locus_tag="ACHAZB_001163"
     CDS             4977..6005
                     /gene="rfbB"
                     /locus_tag="ACHAZB_001163"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFM8613852.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            6103..6954
                     /gene="rfbD"
                     /locus_tag="ACHAZB_001164"
     CDS             6103..6954
                     /gene="rfbD"
                     /locus_tag="ACHAZB_001164"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFM8613853.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            6989..9022
                     /locus_tag="ACHAZB_001165"
     CDS             6989..9022
                     /locus_tag="ACHAZB_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8613854.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            9066..10346
                     /locus_tag="ACHAZB_001166"
     CDS             9066..10346
                     /locus_tag="ACHAZB_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HFM8613855.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LVILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTDREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            10556..11362
                     /locus_tag="ACHAZB_001167"
     CDS             10556..11362
                     /locus_tag="ACHAZB_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8613856.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            11374..12594
                     /locus_tag="ACHAZB_001168"
     CDS             11374..12594
                     /locus_tag="ACHAZB_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8613857.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            12584..14170
                     /locus_tag="ACHAZB_001169"
     CDS             12584..14170
                     /locus_tag="ACHAZB_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8613858.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            14209..16347
                     /locus_tag="ACHAZB_001170"
     CDS             14209..16347
                     /locus_tag="ACHAZB_001170"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM8613859.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKDGIGDFSLRPD"
     gene            complement(16715..17698)
                     /locus_tag="ACHAZB_001171"
     CDS             complement(16715..17698)
                     /locus_tag="ACHAZB_001171"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFM8613860.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
BASE COUNT         5732 a         3025 c         3788 g         5555 t
ORIGIN      
        1 gtattcaaaa tcatttcagt acaaacaggt atggtcgaca aaccaaatga aagaagaata
       61 aataaagttc caatgccttc agcgggcgga ctgcctatct ttatttcatt tgttatatcg
      121 actttattct tattccgtaa aattatccct caatcgtata ttgtaccgat cttgattgct
      181 tctttagtaa tcatagtcac aggagtgctc gatgataaat atgagctgac acctaaacaa
      241 aaaagtatag ggatcttgat tgcttctctt attatttatt ttgtagcaga tatacgtatt
      301 gattccttca cgcttccttt tattggatat atccaactag gctggcttag ttttcccgta
      361 acgatttttt ggatcttcgg tatcacgaat gcggtcaatc tgattgacgg tttggatggg
      421 ctggcggcag ggatttcatt gatcggttta attacgatag gaatcattgg atattttttc
      481 ctccatgctt ctacagtgta tattccagtt gttattttct gtcttgtagc aagtattatc
      541 ggtttttttc cttacaattt ttatccggca aagatttatt taggcgatac aggagctcta
      601 ttcctagggt ttatgatggc cgtcctttca ctacaagggc tgaagaacgt gacttttgtt
      661 tcatcgattt ctttgctgat cgtcatggga gtgccggtaa cagacacgtt ttttgctatc
      721 attaggagaa aagcaaaccg tgtttctttt tctacagcag ataaaaaaca tcttcatcac
      781 cgactaatca gtttaggctt tactcataaa ggtgctgtcc tgacgattta tgcgatggct
      841 ttgatgtttt ctttcacagc tatggtcatg aattatactg gaagaatcgg aacgatcgta
      901 ttgattattg ccatgttgtt tgctgcaatt ttattatttg aattgattgg tctgatcaat
      961 gagaaacatc agtttatttt aaaaacattg cgatttttag ggaataaaga gtaccgtacg
     1021 caagtaaaac agaaatattt gaagaaatgg aaccgtcata tagatgaata acaaattaaa
     1081 ccagccgatc cttattagta ttgtgacggc aaacagtgat aaaatttttc ggacactaga
     1141 taattttatt tcaactgtaa aagatgaaac agcagtaaaa atccgaattt ttgacaacaa
     1201 ttcacttccc gagtacaaaa aaagactaac agagtatagg aaatatccct acatcgatat
     1261 ctattttcat gatgaaaatt ccggatttgg ttatggacat aatcacaatc tgatagctag
     1321 cgattctcgc tatggcatta tttgtaaccc agatatttta gtggatgaag agacagttac
     1381 agcattggtc ttgttattga aagaacatcc ggaatgtgcg atgttagcac ctaagattct
     1441 taatgaagat ggaacaactc agcacctcat ccgtgagcag ctggctgttt ttgattacgt
     1501 tttgcgcttt gtgcctttca aatgggtcaa aaaaaagttt gacaaacgac tggctgtgtt
     1561 tgaatgccga ggattgccta ctgatgctaa tagttatgta cggatgatct caggtagttt
     1621 tatggtagct gatattgata aatttaaaga gataaagggg tttgatgagc gtttcttcat
     1681 gtactttgaa gacaacgact tgtgtttgag ttttgaaaaa gcaggttaca agttattgta
     1741 cacacccttt tatgcagtta tccattttta cgggaagggt gcgcatcgaa atgcaaaact
     1801 gtttcgcgtg tttttgactt ctatgatgaa atttttcaat aagtgggggt ggcgtttttt
     1861 ctgatgaaac cagaaatatt agtagtcgtt gtgctttatc agcaactatt cagtcaatct
     1921 ccagcttatg gaccattaag caaagcactt agcgaaaagc aaattcagct tattatttac
     1981 gataatagtc ctcaaaggca gcaaaatgag ctgtttgaaa aagaaaatat cgtttatcat
     2041 catgatccgc aaaatcctgg tttagcggtg gcttataatt ttgcgttgtc tcaagcagat
     2101 gaaggaacac gttgtctcgt cactctggat caggatacga ggctagtcga cgattatttt
     2161 gaactattgc gtaaagtaac ctttacagat gaatgtgttg ctgcagttcc gatgattttt
     2221 tctgggagcc gacaagtttc tcccctatat tcagatcgtt atatcaatcg tacagctcaa
     2281 gcagtagaag ttgaaggccc aacagctgaa cgaatcatgg caatcaattc aggtgttgcc
     2341 tggtctgtca aatttctaaa aaagattggc gggtttaatc ctgctttttc tttagatttt
     2401 ttggatcatt ggctgttttg gaaagtggat cagttggaaa aaactgtgga gatccttcct
     2461 gtacgaatgg aacatgatct ttcggtttta gactatgaaa aagtttcaag taatcgatac
     2521 cgttctattt tacaagcaga aaatcgattt tatcatgaat atgatcaaag gcatttatcc
     2581 agtcaccgac gacaattatt gttaagaaca gctaaacagt tcttgaccgt caaaaaccgg
     2641 catatctggc ggatgacttt gcgctcatat gttaagaatt ggaaggtgtg aattcgtgcg
     2701 ttcggtttgt atagctacct ttaatggagg cagatattta agagagcagt tagatagtat
     2761 tttatctcag ctttcagcag aagatgaagt agtcatttct gatgatgggt cagtagatga
     2821 tacatgggat atattaactg aatacgcaga aaaagataag cgaatccagc ttttttcggg
     2881 gcctcgtcaa ggattgatcg ctaattttgg ttatgcgatc cagcagacga aaggagatct
     2941 aatctttttg gcagaccaag atgatgtttg gttggagaca aaagtgaaaa agatcactga
     3001 ctattttgag cagaatccgg atcagctagt agtagtttct gatttaatca ttgtggacag
     3061 cgagctgcaa gagatccatg attcgtattt ttcgtttcga aattccaaag aaggatggct
     3121 taataatgtg ataagaagtc actatattgg tgctggtatg gctttccggt cttctttaaa
     3181 acccgatatt ttgccgatcc cgtcagaagt gccgatgcat gatatgtgga ttggtttgct
     3241 gggaggaaag aagactgggt tcctaagaga accgttaact ctgtaccgaa gacatgattt
     3301 aaacgcgagt gagattgaaa cacattctaa attacggcaa aaaatttctt ggcgtgttca
     3361 tctgattacg gcgttgatca aacgaaaatg gtttaaacgt tgataaaaga tgtttaaatc
     3421 cttaatattt ccatgacaaa tataacaatt gatgagataa taacaaagtg aaatttgaaa
     3481 tcaaaaagga gagcagatat ccatgaaggg aatcattctt gctgggggca gcggaacacg
     3541 tctgtaccct ttaacaaagg caacatcgaa acaattgatg ccgatttatg acaaaccaat
     3601 gatttattat ccaatgtcta ctctgatgtt ggcgggaatc aaagaaattc tgattatttc
     3661 aacgccacaa gatacaccac gtttcaagga attatttgga aacggaaatg atttaggact
     3721 gcagatcgaa tatgcagtac aagaaagtcc agatggattg gcacaagcgt ttatcattgg
     3781 agaagaattt attggagatg atagtgtctg cttggtatta ggggacaata tttactatgg
     3841 tggcggtctg tctaaaatgt tgcaacgagc agcttcaaaa gatacaggcg cgacagtatt
     3901 tggctatcat gtcaatgatc cagaacgctt tggtgtagta gaatttgatg agaatatgca
     3961 agctttgtcg attgaagaaa agcctgctca gccaaaatca aattacgctg taactggcct
     4021 ttatttttat gacaatgagg taatttctat cgctaaaggt atcaaacctt cagaacgtgg
     4081 agaactagag attacggatg tcaataaagc atatttggat aaaggaaaac tatccgtaga
     4141 acttatggga cgcggatttg cttggcttga tacaggaaca catgaatcat tgcttgaagc
     4201 atctaccttt atcgaaacaa ttgaaaaacg tcagaactta aaagtggctt gtttggaaga
     4261 aattgcttac cgtatgggct acatcgataa ggaacaattg ctggcccttg cacagccatt
     4321 gaaaaaaaat caatacggtc aatatttatt gaatttggcc gctgaataag gagtccttac
     4381 agatgaaagt aacagataca aaattaacag atgtcaaaat cgtagaaatg gacgtctttg
     4441 gcgatcatcg cggtttcttc acagaaagtt attcaaaagc aaaatttgca gaacatggat
     4501 tgaaatatga ctttgttcaa gacaaccatt cactatcagc tgaagctggt gtccttcggg
     4561 ggcttcattt ccaaaaagga gaagctgcac agacaaaatt gattcgggta gtcactggag
     4621 cagttttaga tgtagtagtg gatatccgca aaggtagccc gacttatggt caatgggacg
     4681 gatttatctt atctgaacat aatcatcgac agttgcttgt acctaaagga tatgcccatg
     4741 gattcgttac tttgacacca aatgtgaact tcctttataa gtgtgataat tactataatg
     4801 cagaagcaga tgggggcatc gcatttgatg atccagattt agcgattgat tggccaattg
     4861 atccatcaag agcgattatg tcggaaaaag atcaccgtca tccgacatta aaagaatttg
     4921 aagcagaaaa tccatttgtt tacggagaaa tctaagaatt aaggagaaat tttcttatga
     4981 aaaacatcat tgttaccgga ggtgccggat tcatcggctc taatttcgtt cattacgtgg
     5041 taaacaatca tccagaagtg catgtcactg ttttggataa actgacttat gcaggaaaca
     5101 aagagaactt ggccggactt cctagcgatc gcgtagaatt ggtcgtagga gatgttgcag
     5161 atgcagaatt agtggaccgt ctagtaaaag aagcggacgc agttgttcat tacgcggcag
     5221 aatcccataa cgataattca ttaaaagatc ctttcccatt tgtacaaacg aatatcattg
     5281 gtacgtatac acttttagaa gcttgccgca agtatgatgt acgctatcat cacgtatcta
     5341 ctgacgaggt ttacggcgat cttccattac gtgaagacct gccaggacat ggggaaggcg
     5401 aaggagaaaa attcactgcg gaaacaccat ataatccttc aagtccatat tcttctacaa
     5461 aagctggttc agatctgtta gtaaaagcat gggtgcgttc ttttggctta cgtgcaacca
     5521 tttcaaactg ttcaaacaac tatggtcctt atcagcatat tgaaaaattt attcctcgcc
     5581 aagtaacgag tatcttaagc ggtattcgtc caaaattata tggcgaagga aagaatgttc
     5641 gtgactggat ccatacgaat gatcattctt cagcagtgtg gctgattttg acgaaaggca
     5701 gaattggcga aacctatctg atcggtgcag acggtgaaga agataataag accgtgatgg
     5761 aattgatctt agaaatgatg ggccaaccaa aagacgcata cgaccatgta aacgaccgcg
     5821 cagggcacga tttgcgttat gcaatcgatt cgacaaaatt gcgagaagag ctaggttggc
     5881 agcctgagtt tacgaacttc cgtgatgggt tggctgacac gatcaaatgg tacgaagaac
     5941 atgaagactg gtggaaaaaa gaaaaagagg ccgttgaagc agcatatgcc aaaaatggac
     6001 aataatacag aggaaatttc actgtattaa tggaaaaaca catgtgaggc aggcgctcgc
     6061 ctgtctcttt tttaaaataa aaatcaagga gcggataacc atatgttttt attaactgga
     6121 ggaaatggac aattaggtac agaattgcgc catttattag atgaaaaaaa tctgccatat
     6181 gtttcaacag atgcaaaaga cttggatatc acagatgctg agaaaacaat ggcttatatt
     6241 acagaattaa aacccgatgt tattttccat tgcgcagctt acacagcagt agataaagca
     6301 gaagaagaag caaaagaatt agatgagaaa atcaatgtcg atggcacaag aaatgtagca
     6361 cttgcagcaa aagctgtgaa tgcacaattc gtctatatca gtacagacta tgttttcgat
     6421 ggaaaaaaca aaaatgtaga atacgaagta gacgaccaaa cgaatccact aaatgaatat
     6481 ggtcgtacga aattattagg cgaaaaagca gtagaagaga ttttagaaga ctattatatc
     6541 atacgaactt cttgggtttt cgggatctat ggacacaatt tcgtcttcac gatgcaacgc
     6601 ctagcggaaa cccgcgacaa actgactgta gtaaatgacc agtttgggcg accaacttgg
     6661 acacgtactt tggcagaatt tatggtatat gtcatcgaag aaaaagcacc atttggtatt
     6721 tatcatttgt cgaacgaaaa tagctgttct tggtatgagt tcgcaaaaga aatattgaaa
     6781 gatacagatg tagaagttgc acctgttacg tcagaagaat ttccgcaaaa agcaacacgt
     6841 ccgcagtatt cagtcatgag cctgaaaaag acagaagcgt taggttttgt cataccaact
     6901 tggcaagaag cacttgcgca aatgctggaa gctgccaaaa aaatgaataa gtaatttcct
     6961 ttttatcaaa tagaatttgg agcatgatat ggaaaaacaa acaagaagtg cacgacgaaa
     7021 agaaaagcca aaagcgaatc aggcagattc gaatttggtg cttgacctgt ttctaaaact
     7081 ccgctatctt attggcgttt tagtcatcgt ggtcatcgtt acttttaatt taaacggaag
     7141 ttcacttggt gtttgggata aatatgtgtc gcaaagagac gatgggaaaa aaactgatgt
     7201 gatttttgga cagaatcggg aagtgcggtc agacgaatgg ctggtgcaga caccttttta
     7261 cctctcacaa gctgaagagg acttttcgtt ggtgaacgag gattattcat taaacggaca
     7321 aaacatgatc attgcgtata attctcctgt aaaggatatt acggttatcg gaaaaccatt
     7381 caactgggga ttctttttcc tcggaagaga tcgaggactt tccttttatt gggcgatgaa
     7441 attagtcgtc atggtcttgc ttggattcga agtactgatg atattaacga agaggaaaaa
     7501 agctctatcg ctaatcggtg catttgcgct gacattttct ccagcagtcc agtggtggtt
     7561 catgcaacat gtgggcgatt tgattttctt tactttggga ctgatgattg ctttttatca
     7621 ttatttctac aatcatgata aaaaatggct acgaacgttg atgatgcttt tggtggttat
     7681 tttcggtatt gggtttattt tagtgattta cccagcccat caagtgatgc tggcgtactt
     7741 gctgattttt tattttgttg gcctgcttat ttactatagc aagaaagttt catgggatct
     7801 gttggatgta gtgttgattg gaggggcagt tttatttatt ggcggagtca tggttcattt
     7861 ctggctaacg tcaaaagacg cattgttagc ttctttgaat accttatatc caggaaaacg
     7921 ggtaagtact ggaggaaagt ggacgattgg cgaatttttt tctttcctta caaattggaa
     7981 aattcctttt gaagatatca actattcgaa taactctgaa gtggccctgt tctatcactt
     8041 ttttccaact gtattcctag caagtccatt tgtgttattt ggcaaaaaag atagcgaaca
     8101 aaagttactg ggacgtattt tgatgatttt ttgtctgttt tctatctttt ggattaccgt
     8161 tgggttgcca aaatggctag cagaaatcac tctgctgtct tatgttcctc cattaagagc
     8221 catccttact tttagttttg cggcttgttt attatcgatc tggtttatcg cttatatttg
     8281 gagagaagat gttattccta tttggtataa attcattttg ttggcagtaa atgcatggtc
     8341 ttatttctat gcgattacgc attcgaaaat ggataattat ttttcggatt ttgaaaccat
     8401 cgcagttgct gtgttgggaa cagtgatttt agcttttgca ttatttggct ggagacgctt
     8461 gttttacctc gcttttgtcg ccttagttat cgtatctggc tttttcgtca atccagtcgt
     8521 agaaggtaca ggagcgattt ttgaaaaaac actggcaaaa gaaatccaaa aaatcgatca
     8581 acaagatcct ggtaaagttt ggttgtctga agatgaatta tataacttca ccccaacatt
     8641 gggagtgaaa tccttcaata cagtacgctt ttatccagat atagctgcat ggaagaaaat
     8701 cgatccgaaa gaagaatacg agaagtatta taatcggtat gctcatacaa gagcttttat
     8761 cgctaaggaa gatacaaagt tcattttaga tcgtccagat atgttttcgg tcacgttgaa
     8821 ttttgaagac gcagaaaaat tagggatcga ctatgtgatc agtaaacgac cattaactga
     8881 ttacaaccaa ttatatgaag cgcagttcac gcagctatat ggaccggata aagacggtta
     8941 tatgattttt aaagtagaat atccggatta tccagatgta ccacaaacac aaactcagga
     9001 aaatcagcta gtcgtacagt aataggctag ctgagaataa tcctgaaata ttagtagaaa
     9061 ggagagtgaa gataattcaa gcaatcgtac aaaaatttgt caacaaaatg agtgtcggtt
     9121 tcttgctttt gttgctgacg ctagtctttt ttgtttggac agcaggggta ggttttccag
     9181 caactttgtt tatatataac aatagttcat tacttatcgc ttttagtgtg gctattttgc
     9241 tcattttaaa tgtttataaa gcaaaaggcg tcgattttct ttatctaggt gttgcgcttg
     9301 cccttttcat tttcttttct tactttcggg aattaagaaa tgagtcttta tttggagact
     9361 tactgtttcc tttggttatt ttattttgtt tagtaattaa atggatcgag tggacaaaat
     9421 gggatcgcgg tatttatctg attgtcttta cggtcttcct cggaatcacg ttatatcgtg
     9481 tatttacaga gatccaagta ccagaaggac aaagcatctg ggctccaggc aattcgcttt
     9541 ctgatatttg gatcaatacg aatacaatcg gaagttcttt gatgactctt gctttattga
     9601 tcagtggatt tgccagttct tttgaacgat ggtatgttcg aatcatcggt gggatagcag
     9661 tcgttgcagc ctttttaggt atttgggtct gccaatctag aggtgcatta gtcgcgttga
     9721 ttgtttttgc gatattggat cttttaccta aatcgttgat gcgtgtcatt cgtgcgccat
     9781 ttattgccta cacggttacg attcttttag ctttgccgat ttcttatctg gcggctgtct
     9841 ctgaaaaagt caatttgttt accgatcgag aagatatttg gcataaattc tatcagacgc
     9901 taggagaaaa atctgaacaa gttttactag gaatgaaaac atttatcttc cagcgaggaa
     9961 accaattttt aggaaaccac aatagttata attctatttt gaatatctat ggattgatag
    10021 gatttgggat tgctgctttg ctgcttatcc tttttattgg tcgcttaacg ttgaaagcag
    10081 atttatccaa cgggcagatg acttttatct gggccttttt tgcggtgatg atgcagtcat
    10141 ttatggagga tacattgact tcatacccat gggttccgat tatttatatc ctgttggcta
    10201 tggccgttca tcgatatgat gaaccagaag ttgcggagga attctatgtt atggaagagg
    10261 catatgacga tatgacttat gatgatacat cttatgagga acaaccagat agcgctcatt
    10321 cttccaggat ccaacgctat cattgatagg cacaaaatac gagagaggct gtgacaaccg
    10381 tcgcagcctt ttttggttta ttgcaattgt ctagcatctt ctgtaaaata gaaacgttag
    10441 agaaaatctt gtatcataga aaattaagaa agaaataaga acgcagctcg gtaccaaaaa
    10501 tatggtaaaa tggcacttga ctgtaattca ctgaaaatcg cttggaggaa cacaaatgtt
    10561 taaagatttg tatttattta tgaaggacat ttatcagaac agaaaattat tgctacagtt
    10621 ttcgatcaat gacttcaaat caagatatgc aggatcgttt ttaggaatct tatgggcgtt
    10681 tatcaatccg ctatttactg tattgattta ttggctggta tttggtgttg gactgaaagc
    10741 acaaatgatc gatgcaaaat acccatttat cgtatattta gttaccggga tgattccctg
    10801 gttcttcttc tcagatgcat ttgcatcaac tacggttgtt tttcgggaat atacttattt
    10861 agtaaagaaa gtcgtattca atattcgtat cttgcccacg acgaaaatcc tatccaatct
    10921 gtacacccat ttattcttca ttctgattgg catagctgtc gcgcttataa atggtatata
    10981 tccttcagca atgtctttac agctgattta ttatttattt tgcatgtgtg ctttcttgac
    11041 aggattgact tggctgacgg catcgatcca accattttta cctgatatca tgcaattaat
    11101 tacgatcgtg ctgcagttga tcatgtggac attaccgata ttatggagcc ctaatcagtt
    11161 tgattcaggt cttgtaagca tattgaaatt aaatccgctg tactatgttg ttcaaggtta
    11221 tcgtgagtca tttttaagtg aagcttggtt ctgggagcat ggaacatata ctttgtattt
    11281 ctgggttttc acgttagtaa tgttcgtaat cggctctgca gtgttccgta gattgaaacc
    11341 acatttttca gatgtgctgt aaggagaata gctatgacag aatatgcaat tagactaaaa
    11401 gatatcacaa aatcttataa tatgtacgca aaaccttcgg atcgatttcg tgaagccctc
    11461 aatcctttta agaaatccta tcatgatgta ttttatgcgt tgaaaaacgt gaatgtagaa
    11521 ataaaaaaag gtgagatgat cggtttcgtg ggagaaaacg gatcgggaaa atctacaatg
    11581 ttgaaaatca ttacgggagt actgacacca acatccggca cgatggaaat cgaaggaaaa
    11641 atctcagctt tgctagaact aggatcagga ttcaatcctg agtattctgg ctatgaaaat
    11701 atctatttaa atggaatggt tctaggattt tcaagagaag aagtcgatga aatggtcgat
    11761 gatatcattg aatttgctga catcggagac catatttatc aacctgtaaa aacttattca
    11821 agcgggatgt tcgttcgtct agcttttgct gtagcaatca atgtgaatcc cgatattttg
    11881 attgtggatg aagcattggc agtaggcgat cttgaatttc aactaaaatg tatggaaaaa
    11941 tttacagaaa tcaagaactc gggaaagacg attttattcg tttctcatga tatcagttcg
    12001 attcgtcgtt tttgtgatcg ttcctattgg ttgaaaaacg gagaagtcgt cgaatatggc
    12061 gatacgatgg aagtaacgac gaattatgaa aactttttaa agaaaaaatc agttaaaaca
    12121 gtggatcgga ataaaaacca agtagaacgg ttcgccgctc cagatatcgt cgacgtgacg
    12181 aaagcggaat tgttggataa agacaaaaat ccggtggata tgctggaaca aggagaaaaa
    12241 ctatacgtta aagtaactta tgaagtaaaa gatgatacaa tcaaaaatcc ggttttagga
    12301 attgcgctac gaacagtcga caatttttat gtatgcggac ttaatactct actagatggt
    12361 atcaagattc cttggaaaaa aggagagaat atcttttatc tggagtatga aaagttaggt
    12421 ttgttagctg gtgaatatta ttttgacgtt gcggtctttg aagaaaatgc aacggttcct
    12481 ttggtatata agacaaagta catgaacttg tttgtcagcg gttcttatat cggagaaggc
    12541 atcgtggtct tagaccataa atgggaagaa gggactcata gcaatgaaat atgattttga
    12601 aatggaccta gatgaacaaa gcagtgtggg aaaaattgcc gcacagatca aaccaggaag
    12661 taaagtttta gagtttggtc ccggaaacgg ccgattaacg aaacatttaa tcggagcaaa
    12721 acaatgtgaa gtgagcattg ttgagttaga taaggaattg ttcgattttg tcagcgaatt
    12781 tgctcaagat ggattttatg gtgatatcga aagttttgag tgggcaaact attatgctgg
    12841 acagacattt gattacgtct tgtttgcgga tgttttagaa catttggtag atcctggaaa
    12901 aacattgaag aaagtcagag aatttttgaa tgaagaagga gagatcctga ttacattccc
    12961 taacttggcg cataattctg tcatgatcga tttgttcaat aaccaattgc cgtgggcttc
    13021 ttatggtctc ctagatgaaa cccataattc tttttatact catgatggct tccaaaaagt
    13081 ttttgaaaaa gcgggattat tcattaatat tgaagattat ttgtacttag ctgtaggtga
    13141 tacagaatta aaatcaactt atgaagaatt gccggaagct gtacgctatg atttcaaaat
    13201 gcgaccattt ggtgaagtct accaatactt tttctcattg atgaaacatc cagtagcaca
    13261 aagttccatt gcagagccac aaaattctaa ttatgtaaaa gtgttagaag taacccagca
    13321 aacgaaacaa gatgaaacaa tccaacaaat accattcaat aatttcacag gggaaaatga
    13381 gacactaagt ttccccgtat cagaaacaac agaaagaatg gtgtttcgct tcgcgcaaca
    13441 acctagcttt atcgaattca gcgcagaagc tgccggagaa aaactaacat ttatcgattc
    13501 gaatgctgta gtcaaaacag taaatgattg ttatttgttt gatgggaaag aattaccaga
    13561 gttcgttcta acggatattt ctgggaaaga agtgactatc cattgtcact atcgctttat
    13621 cggggaattg acaccaacaa tgaaagaatt gttggaaacg attcgtccca tggctgaagt
    13681 cacaaagcag ctttctgaga aaaatgacga attagaaaga gaaaatcatc atcttcttga
    13741 ggaaaataca agactagacc atacgttaaa aacaaccaca aatcgctatt gcacattgtt
    13801 ggaaagtgat gaatggacaa taaaacatag actaggacga cgtaagaaag aaacttcgaa
    13861 aaaaatccaa gaaaaagaac tttctatatg tattgacgag aaaatctggg atgcagaaac
    13921 aaaaatatta aaaatcatcg gatggggaat cgctaattcg gatcgccagc ccttgtccta
    13981 taagttatca gctgaccaat cgccattttt cgaagcaatt cctgtttcac gagaagaagt
    14041 caatcaagca gaacagctcg caaaaggaac agaagcggga tttgagttac gaatcctttg
    14101 cgagcaagaa cgttcattct tagtggaagc agtagctcaa aatggccaat catggatcat
    14161 tgaaatgtaa ataatgatta acttataaag ataaggagta aacgaatcgt gcaaaaaaaa
    14221 gaaattgccg tatttatcga tcaaatcact cgtgaacggg ccactggcga tttgcttatt
    14281 gtgggctggg caattgatga agtgaccaaa gaaatcccta cgataaaggt agaaaaagaa
    14341 aatgtgattg ctgaagcaac acatgtcgta agattggata tcaatcattt atataatctt
    14401 gatgttaaga cccaaagcgg tttcaaggta cgtttatcag ggaaaatgag aggaaaagcc
    14461 atcttagact tccaaacagc gagacatcaa aatggcatag ctgtgaaatt aaacggtaaa
    14521 tacccttatg atgacggcat tgaatcttca tgggaaagaa aaaaaagatt actgaaaaaa
    14581 gggataaatt atgcaaggac tcatggcgta aaaaaagcga tccgtcgggt caaattggaa
    14641 ttgaagccag gatcgatcga ctatgcagag tggatcagtc gtcatgagaa accagatttg
    14701 aaagagcaaa ggaaagaagc acaaggattt gcgtaccgtc cattgatttc catacttatg
    14761 cctgtctata acgttgaaat caaatggctc gaaaaatgca ttgattcggt actggatcaa
    14821 acgtatgatc attgggaact atgtatcagt gatgatgctt ctactgatcc agctatccga
    14881 aagtgtctgg aatcctatca agcaaaagat gatagaatca aagtcgtttt ccgaaaagaa
    14941 aatggacaca tcagtctggc aactaattct gcattggaaa tagcagaagg agaatttata
    15001 gcactcttag ataatgatga tgaattaccg ccatttgcat tatatgaagt ggcaaaagtg
    15061 ctaaacgtac atccagaact ggatgtgatc tacagtgacg aagataaaat cgatgcagat
    15121 ggcaatcgct ttgatccgca tttcaaagca gactggtcgc ctgatacatt gatgggaaat
    15181 aattatatct ctcacttagg cgtatatcga acaagcattg ttaaagaact tggcggtttc
    15241 cgtaaaggct atgaaggttc tcaagattat gatttggtcc ttcgtgtgac agaacaaatc
    15301 cctgcagatc atatttacca cattgaccga gtcttgtatc attggcggac gatcccagga
    15361 tcaactgcaa gtaatggcga agccaaaagt tatatctatg attccggagt gaaagcattg
    15421 acagatgccc tgtctagaag aaacatcaaa ggaagcgtac gtcctggcag aatttctgga
    15481 ttttatgaga ttgcttatga cgtcttgcaa gaggatttgg tcagtgtgat cattccaacg
    15541 aagaatggct atgaagatct aaaaacttgt gtggattcga tcatcgaaaa aacgtcttat
    15601 tcaaattatg aaatcattat tgcggataac ggaagtacag atccaaaaat gcaagaatta
    15661 tttgcagaat acaagcatca gctgaaagac cgctttatcg tagaattgat tgacattcca
    15721 ttcaattatt ctcgaattaa caatttagca gcagaaaaag caaacggcaa atacttttta
    15781 ttcttgaata acgatacaga agtgattgag ccagattgga tgacggtgat ggtttcttat
    15841 gcacagtttg atcgaatcgg ctgtgtagga gcaaaactgt tttatccaga tgatacaacg
    15901 cagcatgcag gagtattatt aggaatcggt ggtgttgcag ggcatgcatt gaataactat
    15961 gatcgaacac attgtggtta tttcggccga ttggtcattg acgtcaatta cttagcagtc
    16021 actgcagctt gtatgatggt caaggcagct gacttcaatg ctgttaacgg gtttgatgaa
    16081 acattagaag tagcattcaa cgatgttgac ttgtgcttga aagtttatga actgggtcgt
    16141 tacaatgtct atgcacatca agcagaattg taccatttcg aatctaaatc aagaggttat
    16201 gaagatactc cagaaaaaca aagacgtttt gctggtgaaa tcaaaaaaat gcaagataaa
    16261 tggccagcat atattgcgca tgatccattt tacaatgata atttaacaaa agacggaatc
    16321 ggcgactttt cgttgagacc agattaaact ataataaaaa gctgttatgt tgtgattcgc
    16381 tcgcacataa cggcttttta tagttttttg tcaaaaaaag gcagaaggtg ccgactcaaa
    16441 aaaataagtc gaggttggga cagaagtgtt taactccgag aaataagaag aaattcacga
    16501 aaattgcttt tcaaattttt gtgaatttcg gcttatttaa gaagaagttg cttctgctcc
    16561 caccgtttat tcgtttttaa agaatgagac aaaagtgttt cttacttttg tcccacgctc
    16621 ttattcatgc tcagagcgcc aatagataca aaatcatagc caaagtttca cgaaccctag
    16681 ctgtcaaaga atcgatattt tattttaata taggctattt taaaataaat ggtttaaggt
    16741 attcctctac aaaagccact ctatcaatta tcatgggttt aaaataagaa gtgatggaga
    16801 taaccagatt gttcttagcg tcaatataaa gaacatttcc actattacca attgctgaat
    16861 atatttcgtt ctcagaatct aatacccacc ataaatagcc atatctcata ttttgtaagt
    16921 cttctaaagc actggttcta ggtttgaaca tctcgtttat ccacatgggt ggtacgattt
    16981 gttttccttt atacatccct ttatttaggc ataatacacc aatttttgcc agatcacttg
    17041 ccgataaaca caaaccatat cctgccgtcc cgatatttag tggatcacaa aaccagacat
    17101 tggttttagg agacttatcc attgtaaact gcttatgttc ttcagctgtt tcagcataat
    17161 aattgacatg gtttggtatc cctataggat caaatagata cttattggca aaatcaacag
    17221 ttgttagctt acttgtctga tacaacaagc cagttaatat gtgtaaacag acagtttgat
    17281 agttgaattc gccggttata ccttttcttc ctcccaataa atctagtgat gtttttgtcc
    17341 aatcttcact tgaacagact tttgtccatg gatcgccttt acatttgtag ggtgctttca
    17401 ttgttagcaa gtgcttgatt gtaatagcgt aaatagtttt ctccccgcgt ttcactttat
    17461 actctggaaa ataatctaat actttatcat cgatactttt gattaatcct tgctctaaag
    17521 caatgccaat cagcaaagat acaatacttt tcgttgctga catgatatga caagtatcgt
    17581 cttttttata accattccat tcatctgtat aaacttctac cccatcctta tatgcgacaa
    17641 tttgacatat atttggttgt cgaaaaccaa taaaatcatg caactcttgt ttattcataa
    17701 gatattacct cactttcttc tataagcata atgtcattaa aaatcgattt caattttttc
    17761 gattcttcac gtaatacagg tattcttatg gactagacag cgctttggga agatggattc
    17821 tctgaaaaat ataaaggttc actttatgag tggataaaaa aagcagcaaa tacttcgact
    17881 tctttaattt accaacatta gtgaaaatcg attcatctgg agatttgtaa tttagagtcc
    17941 ttgtcttgat tagaggtgta taaagctttt tattagacat ttttaaattc cattacaatt
    18001 tgcttataaa ctaggttttt aaaggtgact atggtaagat aatagagata ttattttgtg
    18061 attaggagtg tatatatgaa taaaaatgga gaatggagtg
//
LOCUS       DAYYCY010000043        17948 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000043 DAYYCY010000000
VERSION     DAYYCY010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17948
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.043"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            54..1394
                     /locus_tag="ACHAZB_001172"
     CDS             54..1394
                     /locus_tag="ACHAZB_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8613861.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            1612..2055
                     /locus_tag="ACHAZB_001173"
     CDS             1612..2055
                     /locus_tag="ACHAZB_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HFM8613862.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(2559..3977)
                     /gene="nhaC"
                     /locus_tag="ACHAZB_001174"
     CDS             complement(2559..3977)
                     /gene="nhaC"
                     /locus_tag="ACHAZB_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFM8613863.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(4380..5603)
                     /locus_tag="ACHAZB_001175"
     CDS             complement(4380..5603)
                     /locus_tag="ACHAZB_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFM8613864.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(5634..6473)
                     /locus_tag="ACHAZB_001176"
     CDS             complement(5634..6473)
                     /locus_tag="ACHAZB_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFM8613865.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(6490..7170)
                     /locus_tag="ACHAZB_001177"
     CDS             complement(6490..7170)
                     /locus_tag="ACHAZB_001177"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFM8613866.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(7172..8245)
                     /locus_tag="ACHAZB_001178"
     CDS             complement(7172..8245)
                     /locus_tag="ACHAZB_001178"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFM8613867.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(8301..8539)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(8582..8815)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(8892..9563)
                     /locus_tag="ACHAZB_001179"
     CDS             complement(8892..9563)
                     /locus_tag="ACHAZB_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8613868.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(9650..12298)
                     /gene="mgtA"
                     /locus_tag="ACHAZB_001180"
     CDS             complement(9650..12298)
                     /gene="mgtA"
                     /locus_tag="ACHAZB_001180"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFM8613869.1"
                     /translation="MEKKKDTQKIYAAFAGKTKQELFELFHASANGLTEEQIERSREN
                     YGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPGDKDLFGVAII
                     FTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEEIVCGDLVRLS
                     AGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSYENLVFMGSNV
                     ISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKFMSLMAPAVIV
                     INGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGTVIKNLNAIQN
                     FGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQTGLKNLLDVAI
                     IDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGAIEEMLNISKF
                     IDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFSVKDESDMVLI
                     GYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLEINELVTGEKI
                     SEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGINDAPAMKVADI
                     GISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKATASSNFGNMFS
                     VLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKWNASSIGEFMV
                     YFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAGWFVESLWTQT
                     LVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLMPLPGNYWLWL
                     FATVIAYLALVTAVKKIYIRRFQELL"
     regulatory      complement(12640..12814)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            12987..13424
                     /gene="ndk"
                     /locus_tag="ACHAZB_001181"
     CDS             12987..13424
                     /gene="ndk"
                     /locus_tag="ACHAZB_001181"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFM8613870.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(13446..14312)
                     /locus_tag="ACHAZB_001182"
     CDS             complement(13446..14312)
                     /locus_tag="ACHAZB_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFM8613871.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(14472..16565)
                     /locus_tag="ACHAZB_001183"
     CDS             complement(14472..16565)
                     /locus_tag="ACHAZB_001183"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFM8613872.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(17314..17937)
                     /locus_tag="ACHAZB_001184"
     CDS             complement(17314..17937)
                     /locus_tag="ACHAZB_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8613873.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
BASE COUNT         5161 a         3718 c         3164 g         5905 t
ORIGIN      
        1 tatttattct taatataaat tttgattgtg aatttaaaag aaagaggtca tttatggaag
       61 ataaaaaatt gaaaaaacaa cttagctcca gacatatcac catgttagct ttaggtggag
      121 caatcggtgc aggtctattt aaaggaagtg gcgaagcgat tgggatcgcc ggcccttctg
      181 tattgattgc ttttttgatt ggtgggctga ttttatatat cgtgatgaaa ggtctgggaa
      241 aaatcgtttt aagcggtggc gacacgcatc acggattatc tggtttgata cgcccttatc
      301 taggtgctca ttctgctgat tttactgatt gggtctactg gtctatgtgg attatcaata
      361 tcatcgctga agctgttgca gctgcttcct ttttgcagtt gtggttcccg cacattccag
      421 cctgggtatt tgtttttatg ttggctgttt taactacgat catcaatctt tattcggtac
      481 ggttattcgc agaaacagaa tattggctgg cttttgctaa aatcagtgtc attatcttac
      541 tgatcatttt tgctacttat cttgtcggtc agcaaatgtt gggcacaggc gttttcccta
      601 cccttcagca attgactgac catggtgggt ttactcctca tggtatgaag ggaatcatca
      661 gttctttgct tgtagttatc tattcttacg gcggttctga actgattgct atcacggtaa
      721 gcgaagcaga tgatcctaaa cgtgcgattc ctaaagcaat caaaggtgtc atgggaagaa
      781 tcatttcctt ttacatcatt cctttgtttt tgcttttgat tctttttcct tggaatactt
      841 tagcaggcac gaatgtcagt ccttttgtga tggtatttga aaaaatgaat atcccatttg
      901 cagcggatat cgtcaatttt gtgattgtcc ttgctttgtt ctcatctatc aattcgggtg
      961 tgtatgcatc ttcaagaatt ttgttcttcc gtttgaagga tcgaaaaggt tcctctagaa
     1021 aactagctgt tttaaacaag catcaagtac cgcaacgtgc tgttttattt tgtaccagtg
     1081 ttctttatct tggtgtagct ctttcttatt ttgtcggtga caaattattt ggctatctgg
     1141 caggttccct ttcgtataca gtattattga tctggatcat tatcagtgcg gcatcctttg
     1201 tactggcact aagaaaagga acattatggg ataaaggaat cagctttttt gcattagctg
     1261 tcttaggatt gattttcctt ggtatcctgt tcacgaactc cattggggta actgtattga
     1321 ctggcctgct ttacctgttc atttatttca gttatcaaaa aaagaatgat gcgttcgtat
     1381 tgacgaatga atgaaatcga tagaaaattt tattttattt atgtggaata gatggagcta
     1441 ttgaagaaag gagttgtgac aaaagtcttg ttgcaactcc tttttccgaa taaacagtct
     1501 catgacctct tggtaaacgg tgttcaaaaa ccctctgtgc aaaataattg attgttctcc
     1561 atgctcatga catgatagaa gaaaggaatg aaaaaaagga gtttgtttta aatgaaaaag
     1621 tattggaagt ttttgttgat gatcggtgtt tcaacggtag ttatgtttgg tatgatgtac
     1681 ttgaatgtct atgcgctcga tcatatcttc ttcagtcgta cacgagtatt catggcgttg
     1741 atgatgggtg cagtcatggc agtcatcatg ctgttgttca tgtggaaaat gtatgaaaac
     1801 aaaaagataa atgttatgat cttagccgct agtcttcttg tttttgccgg ttccttgttt
     1861 atggtacgaa gccaaactac tgtcaacgac acagcttgga tgaaggccat gattcctcat
     1921 cattctatcg ctatcctggc aagtaaacga gctgatttaa aagatcctga agtaaaaaaa
     1981 ttagcggaag aaatcattca ggcacaagaa gaagagatcg acaaaatgaa aaaaatgatc
     2041 aaagaaatgg aatgaccatt atccgtccaa ataaaaaaag aggatgttcc agaagttatc
     2101 agcttcaaga aataagaaga agctacttct ggaacgccgt gaataagggg ttgtgaaaaa
     2161 gctgccctgc atcaagtaat aagaacagcc aaacaaaaat agcttctcct atttttcgtt
     2221 tggttaggct tattaccgca gatgcaagct tttgaacacc gtttattaag aggttgtaaa
     2281 atcagcgttc tgacctgagt attagagaga caaatggaaa aatagttcct catattttgc
     2341 cattttttga gctaataccg agggtcagct gattgaacac cgtttatcga gaggttgtga
     2401 aaaagtcgct tagtttcgcg tattaagaaa aattttgtga aatggctttc cacatttttg
     2461 tataaatttg gcttaatacc gagaaactgg cttttgaata ccgtttattc gtaaaaggga
     2521 ttgtgacaaa agtcttgtca caatcccttt ttatgttttt attgttctaa ttgcgctgtc
     2581 ttgcttactg cttcttgcgg tttcacaaat ttttttgaga ttccccaacc tgtgaaggca
     2641 ctgaccatcg aaaacacagg agaaagcaaa ctgaaaaata cgaatggcaa gtaagtgagt
     2701 gtaggaatac caaatgtact tgctacgaaa cttccggcaa ttccccaagg cactaagtag
     2761 ttgattactg ttccaccatc ttctagcgtt ctacctaata cagttggatc aaatcctgct
     2821 ttttcataag tttctttaaa tgcattacca ggcagaataa cagataggaa ttgttcacca
     2881 ataaaaatat ttactccgat acttgtcatc aatgttgtaa aaatcaaatt tggtgtgttc
     2941 gtcatctttc ttgatactcg ttccatcacg gttgagatca agccaaattc gatcaacaat
     3001 ccacctaatg acaaagttag aataatcaat gacacagtag gcatcatact ttcgattcct
     3061 ccgcggctaa gcagtgaatc gatttgttgg tttccagtat ttgaaacaaa ccctgattcg
     3121 atgaccccag ccaacttcgt taaggaagtt tgtgggtttt gaataaaaat cattcctact
     3181 gctacagcga tattcaacaa aatcgttaaa attgccggca cctttttcca tgcacacagt
     3241 aacattaaaa ataaaggaat cagtgaccag attgaaatag taaagtttgc ttctagaaca
     3301 tcagtcactt cacgaataga tgaaagactt gcggctttac ttgtgtgtcc taaaatcatg
     3361 aataaaaaga aagaaacaac aaatgccggg acagttgacc acatcatatg cttgatgtgt
     3421 ttgaacaaat cggcatcaac aatagctgct gctagatttg ttgattcgga taatggagac
     3481 atcttatctc caaaaacggc acctgaaaca atagctccaa caaccagcgc tggatttaag
     3541 cctagtgttg tcccaatacc gaaaaatgca atcccaatcg ttgacataac agtaaaggca
     3601 ctccctacag cgctacctac gattgcacac acaacaaata cagaagggac aaaccactgg
     3661 acactgatca atttgaaccc aatgaccatt agagttggaa tgatccctgc ttgtatccat
     3721 acagcaatca atgaaccgac taaaataaaa ataaagattg ggatgattcc tggtttgatg
     3781 ccattgatga ttccttcgtg cagccaatct gtcgggtatc tttttatcac tgcatacaac
     3841 atgaccattc cgatcgctgc taagatcgtg atattaggtg agacctttaa tccgacaacg
     3901 ccaatcgcaa tgcctaaaac aataaccatt aatacaagtg ttgcttctaa aaaacttggt
     3961 gttccttttt ttcccatgat aatctctcct tttttagttg ttcttatgtt ctaaaaaagt
     4021 taagatatcg gattactccg ccgcaattca tccgcgattc ctcctttata aaaataaaaa
     4081 gacctgtttt aaactaagga cgaatgactc cgcggtacca ccttaattaa aaacagatct
     4141 gtttttcact ttcaaacttt tattcaaatg tatggacttt ccatgtaatt cgtcaattag
     4201 gcttgatcgg ttcacagcga ccaccgactt tctgaacaaa acacctaacc gctactgcgt
     4261 gaaaagttga cttgtacgaa gtttaccttc cataagatca tttgtcaatg aaaaagaaaa
     4321 tattttttca tttttctgaa aattattttt ataactttca cacacaagtt tattttttac
     4381 tagaaagtag cgccctcttg tttttttctt gctactattt cttgatgttc ttgcttgatt
     4441 ctttctaata gctttggctc tttcaacaga tcaagagctg tattggcgag tagttctgct
     4501 gcaatcgcaa tagaagcaag gcctttgtca cttttagcag cagctttgaa ttcttctgtg
     4561 tgtccggcaa tatatgtctc agagattgct accgtaggct ggattgttgg tatgacttgg
     4621 ctgacatttc ctacatcaga agaaccgaga cttgttcttt gttccgtttc tatttcttca
     4681 tctggaacac ctgcttcttt tgcatgctgg aagaataagt catcaaaaga aggagtaacg
     4741 atgacatcat caactgcatt ttggaacaat ccaaactcaa aagtcgtgcc cgtacttatc
     4801 gcagcacctc gtacgatatt ttccactttt tcatatactt tatctaatgt ttgccgattt
     4861 cctgcacgta aatagaaacg tccagcagcg taatctggaa tgacgttggc agctactcct
     4921 ccgtctgtaa tgatcccatg gatattgaca tcttttggca attgcaagcg tagagcgttg
     4981 attccattaa acacttggat caatgcttcc aaagcattta ctcctttttc aggtgctgct
     5041 gctgcatggg aagcgacacc gtaaaatttg atgtcgactg gatcattcgc taaagattct
     5101 gtcgttttcc cataacgaaa agctggatgg acgcataacg ctacatcgac atcctcaaaa
     5161 aaaccttctc gaacaaagct gcctttagct gatccgttct cgcccccttc ttcccctggg
     5221 gtgccatata cacggatttc tccaccagtt tcatcaatga attgcttcac tatgctggcg
     5281 gctaatacgg agtaagtacc aaacaaatta tgcccgcatg catgaccaat acctggtaaa
     5341 gcatcgaatt ctgccaaaaa ggcaattgtg ggaccgggtt tattggaaat ataacgtgca
     5401 tcaaaggctg ttcggtgtcc agctacttct ttttctaccc aaaacctttc tttttctaat
     5461 tgtttaatca acgctgctga ggaataaaac tcatagttac ttacttcagg atgttcgtgg
     5521 atatctagag caagtgcttg atattctggt aaacgatatt ggattagatc cttcaattgg
     5581 tttgttgaga ttgttgttgt cataaaaaat tcctcctatg ctagtcattt ttcttatttt
     5641 ctcccgaatg tttcccatgc aggcacgctt gatccttttg atgttttctc gattactttg
     5701 actgtgtctt ctgtttgata agcgtctact attttttgat acgttttatt gttttcatct
     5761 tcttttcttg caacgatgat attcacataa ggacgtgcat ggtcatccac tggttctaag
     5821 aagatggcat cagaagcagg attaaatcca gcatctacag ctacaccgct attgatcact
     5881 gaaatgtcta catctgacaa agcacgtgct gtttgggaag catctaattc agaaatgtct
     5941 agattttttc tattctcggt aatatcagaa acagtgggcg cttgtccttt tgccttatcc
     6001 actttgatca atccagcagt ttgcaataac agaagagctc tgccgccatt tgtaacatca
     6061 ttagggattg ctacttttgc tccgtctttg atatcagcta catccttcaa agtgttagaa
     6121 taaattccca gcggtgcatt gacagtattt ccgatcgata ctaaatcagt tccgtgttct
     6181 ttattgtaat tgtctaaaaa gaattgatgt tggaaagaat tcaaatcaat cgatccatct
     6241 gctaaagcca catttggttg attgtaatct gtaaatttca cgtattccaa attaatgttc
     6301 tcctttttca aacggtcttt cacgttgtcc catacatctg tatcttctcc aataatccct
     6361 agttttacga tagtttcttt tttgtcagtt gtatctgctt ttccttggct acaccctgcc
     6421 aaccaaataa ttgctacaaa tgtaatgact acaaaacgaa taattttttt cattttttct
     6481 cctccacttt caatgtgtaa tttttttaat cagttgatta ctgataaatt gacttaaaaa
     6541 aactaagatt agaatcaata atgtagccac aatcgttaca tctgtttgga atcgattata
     6601 ccctctcgat atagcaagat tccctaatcc gcctgcacct actgctccag ccatggtagt
     6661 cagtccgatc acgttgatga tagtcaacgc cgagacccga atgataccag gcaaaccttc
     6721 taacaaatac acacgtaaaa tgataccgat tggactggtt cccatcgctt ctgccgcttc
     6781 gatcacccca ggatcaactt ctagcagtgc gttctcgatt tgacgagcaa aaaacggaac
     6841 aactccaaaa atcaatggca ctaaagctgc tttcgcacca atcgtcgttc caactaagaa
     6901 acgagtagtc aatgctaaaa gagaaagcaa gataatgaaa gggatggaac gaatgatatt
     6961 gatcagctta tcaagtattt caaatagcag acggttagct agtaatccat tagggcgtgt
     7021 cactactaac gtcacaccta agatgattcc aatcatcccg ccaatgaatg ctgtccaaaa
     7081 agacatatat agtgtttgct tcgtcgcttc gacaaattca tccggaatca aataaacatt
     7141 gggcaaatat gttttaaaga aatcagtcat tttatcttcc tccttcgatc actttgatac
     7201 gactatctaa atcgtttttt tgtaaaatat ttatttttac accagatttg atcaaataat
     7261 ccaatgcttt tttccgctgt ttggtttctc cagctaaagt aacgatcaaa ctccctaacg
     7321 gcgtttcttg taataactct acattgccat ataaaatatt cgctgttact tgatatttgc
     7381 tgtacagatg agcgattaga ggttcgttcg tctgatttcc aatataactt aattccacca
     7441 gccatttatt ggcgatctga tcggcaaaag cagaatgggc aatgatcgtt tctaatgctt
     7501 ggtcaagatg agtagctgtt cgaataaaat ctttggtcaa ttcttcttca ggattgctga
     7561 agatctggat gctgctacct ttttcaacga ttcttccatc ttccataacc gctaccttgt
     7621 tgcagatttc tttgacaact tgcatctcat gagtgatcag gacgattgtt aaacctaact
     7681 ggcgattcaa tttttttaac aatgcaagga tctgaagggt cgttttcgga tcaagtgcac
     7741 tggttgcttc atcacataat aataccttag gttcattcgc caaagctcgt gcgattgcta
     7801 ctcgttgttt ttgtcccccc gacagttgtt taggataagc agatgctttt tcttccaaac
     7861 cgacaagttc cagtagttca ttcacttttt gacgacgttc ctgtttcgat tttccagaat
     7921 actttaaaga aaaatcaacg ttgtcaaaaa tagttcggct ttccattaga ttgaaatgct
     7981 gaaagatcat accgatggcc tttctttctt ttcgcaattc tttgggagag agctgactca
     8041 gattcgtctg attgacaatg acttctcctg ctgttggttt ctgcagcaaa ttcatcacac
     8101 gaactaacgt acttttccct gcacctgagt aaccgacgat tccataaaca tcgccttttt
     8161 caatcgttaa atctacagaa tctactgcct gtatctcttt ttctttttgt ttgaaacata
     8221 cagaaacatc tcgcaattca atcattaaaa ttcctcctta tctaaaaaaa gcgtcctcaa
     8281 gaaaatgaaa atgtcatttt cttgaggacg cttttttgcg tgttaccacc tcatgttcaa
     8341 gctgtgttca cacacaactc ctcttcaagt acgatcatac tctagcaaga tatcggtcgc
     8401 agccgtcatc gccttacatc tatgctcggg atgaattgct cgatggccat tttccaatag
     8461 tcgttccttg cttcttctca gctaccgaag ctctctgtca aaggttcttc tatcgtactt
     8521 accatttcaa tgcaaataat attcaaaaaa aaagtccttt tactaacata aactatgtca
     8581 gtaaaaggac gacgtatcgc ggtaccacct tttttcggaa gtttccttcc ctcaagccca
     8641 acatctcaaa aagagagatt ggcgttcgtt ttaacggtcg aacagttccg aatttgccta
     8701 cttatcgaca aattattctc caaggccatt ttcagctttt cttacttgac tcttctcacc
     8761 agccagagcc tctctgcaaa gtttccaaac ttactttcct tttcaacgaa atcgatattt
     8821 tgttgttggg attaatctac atgaggaaaa ctgaaaagtc aagtacgaaa ttcaaattat
     8881 tttttctata tttatttttc tgttttttta tttaacattt caataacagt tcgaagatct
     8941 tttccttccg caagaagttt caatttttcc ttttcttcga cggtataatt tcggacaaaa
     9001 ctggaatcag gtatcaaaac aacgcctaat cctgctcgtg aagcagctgt tgctcctact
     9061 aaagaatctt ccactgcaaa ggctgaacta gcttctaatc ctgttcgttc caaagctgtc
     9121 agataaggaa tcggtgaagg cttgttctcc gacacttcgt ctccgaatgt aaacgtgtcc
     9181 aagtaatcag ctaatttaaa gtgatccaag atatctgttg ctcttttcgt aacagtggtc
     9241 gtagcaatgc ctagtttcat atgacgttct tttaagtctg ccaatatttc ttttgcatat
     9301 ggttttaagt ggatggctcc gcttgtcagc tgttgataaa aatattcttc acgcttcgct
     9361 cggacttcct gtactttttc agggctacct gctgctttca ctaaaatatc aaaactttgt
     9421 ttccagtttt ttccgcccca agcgtcaacg atttcttttg ggatatggat atcaaattgc
     9481 tgaaatcccc aaagccagcc atcacgatac gttttttctg tgtcaactaa caaaccatct
     9541 aaatcaaata ttgcaccttt catctataca ttaatccttt ccaaaacaat acacatagtt
     9601 tagcacgact ggtccaaaaa gaacagaagc aaagaaccag ccttctcgtt tataagagtt
     9661 cttggaatcg acggatatag attttcttca cagctgtcac aagcgccaga taagcgatga
     9721 ctgtagcaaa taaccataac cagtaattgc caggaagtgg catcaagcct aattcagcac
     9781 caaaacttgt aaaaggaagg acagaaccga caataattcc taatgtagtg ataacaaaca
     9841 tggcaaatgt tgcgttgctt tggataaatg gtactttcgg tgtacgcagt gcatggagca
     9901 ctaaggtttg tgtccatagt gattcgacaa accatcctgc atggaataat gcgatgaaaa
     9961 tcactcgctg tgaactatcc aatgtatgga aacttccgcc cactacagca ggacaaataa
    10021 caaaatacat caacaagtaa gtagtaatat caaatatcga acttgttgga ccaaagtaaa
    10081 ccatgaattc cccaatacta gatgcattcc atttttttgg ctcgtccaaa tattctttat
    10141 ccatccgatc ccatggcaag gaaatgcagg aaacatcata gatcagattc aaaaacaaaa
    10201 gttgtaaagg cagcattggt aaaaatggca ggaacgtact tgcaatcagc acggaaaaca
    10261 tattgccaaa attagaactt gctgtagcct tgatatattt catgatattc ccaaaagttt
    10321 ctcgtccaga aagaaggcct ctttccaaaa tcgtcaaatc tttttctaaa aggatcacgt
    10381 ctgcagattc tttcgcaata tccacagctg tatctacaga aataccgata tcagccactt
    10441 tcattgcagg agcgtcattg ataccatctc ctaaaaatcc gacagtatga ccatttttac
    10501 gtaaagctgt cgtcaaacgt gctttttgct gaggattcaa ttttacgaat acttcatggt
    10561 cttcagctac ttgtgctaat tcatgatcac tcatttccga gattttctca ccggtaacca
    10621 attcattgat ctctagacct acttgacggc acactgaacg tgtaactaat tcgttgtccc
    10681 cagtcagaac ttttacagct acaccatgtt ctttcagagc tttcaacgct tgtttcgttg
    10741 tttcttttgg tggatcaagg aaagcaagat aaccaatcaa aaccatgtct gactcatctt
    10801 tgactgaaaa ttctccaacg acgcttggat tggtcttttg tgcgacacca atcactcgta
    10861 agccatcttc attaagtgac tgtacttttg caagtacggc ttcttttcgt tcttttgtta
    10921 atggtgtaac agtaccgcca agatcaataa atttagaaat gttcagcatt tcttcaatcg
    10981 cgccttttgt gatcatttgt gttttaccag ctggatcagc aaccacaaca ctcatacgac
    11041 gacgttcaaa atcaaaagga atttcatcca ctttttgata attgatctta tctgtatcta
    11101 aagtttctgt tgcttcatca atgatagcca catctaacaa atttttcagc cctgtttgat
    11161 agtagctatt tagatacgca tggcgcaaaa cacgatcatc ttcatttcct tcacaatcca
    11221 tatgatattc aagaatgatt ttatcttgtg tcaatgtccc cgttttatcc gtacataaca
    11281 catcgattgc accaaagttc tggatggcat tcaaattttt gatcactgtt ccttttttgg
    11341 ccatcacact tgctcctttg accaaattcg tcgtaacgat cattggaagc atttcaggtg
    11401 ttagaccaac tgctacagat agtccaaaaa gaaaagcttc tagccaatcc ccttttgtaa
    11461 gcccattgat gacgataact gctggagcca tcaatgacat aaatttgatc agtagcatgg
    11521 atgttttatg gatgcctaat tcaaagcttg attgaattgg tttttcagat agcgtttctg
    11581 ctacatgacc aaatagtgtt tcatttccaa cagaaacaat gattccttcc gcacttccac
    11641 tgatgacatt acttcccata aagacaagat tttcgtaact tgtttcgctt tgctgatcgt
    11701 cttcacatac tttcgcatgc ttttctactg gatagctttc ccctgtcaat gctgcttggg
    11761 aaatgaataa atcctttgcg gacaataaac gcatatccgc tggtatcata tcccctgctg
    11821 aaagccgaac aagatctcca cagacgattt cttccatagg aatctctgtg tactttccat
    11881 tacgttttac agctgccgtt actttcacaa gtgatttcag tttttcagcc gcttgattgg
    11941 aacgtacgga ctgaatcaac gtcattgtcc cgctgacgat aaccatagtg aagataatgg
    12001 ctacaccgaa aagatcttta tctccaggag ctgctaatac atattccgtg atgaatgaaa
    12061 taattcctaa tgcgatcaac actaatgtaa atggcgtgat gtatgctttc aatatttcta
    12121 caatgaatgg tgtttttttg ccatatgaaa tcgcattttc accgtaattt tctcttgaac
    12181 gttcgatttg ttcttctgtc aatccatttg ctgatgcatg gaatagttca aataattctt
    12241 gtttcgtttt tccagcaaaa gctgcataga ttttttgtgt atctttcttt ttttccatct
    12301 caatctctcc tttgtgtcca aaagacagct gttctcgctg cttttggtct ttagctattc
    12361 atcggatcga tagagaaaaa actgaaaggg gctgagaaca gatagataat agccctggcc
    12421 tttcctctag atccttgttt ttcgaatgac tgtcatccat ttgttctcat ctcctttact
    12481 aagtaatttt aattcatgac ttccaatcat ttttcgaata gttatttttc caattagacc
    12541 acctcctttt tatttttcac cgatggggat ttgcggcaac aaaaaaacct ctcgccaaat
    12601 actcagcgaa aggtttgaaa aagcatatta aaaaaagctt ctcagatctc atcgttgagt
    12661 tttgacactg tacaacgtaa aaagcagata cctgctttta gctaccttat gaaaagcctt
    12721 atctcgacaa ttcctgttct acccattggc gtctctcgac gtttttgggc agtagcctat
    12781 gtttgtatag gagcctcacc taacaaatct tattcggttt tccttcgtta atataacaac
    12841 taaaaaagag gatgtcaaat caattttcca tgtttttcat acttttttta aaatacacac
    12901 gttatattcc tgtctcacgc ggttttattt gtctcatttt ccacctcccg ctataataga
    12961 aatatagtag gaaacgaggt gctgttatgg aaagaacact tgttattatc aagccagacg
    13021 gcgtgagacg ccatttagtc ggaagcatca tccaacgttt tgaagcaaag ggactggcca
    13081 ttgctgaaat gaagtttgaa actatgactc ctgaattagc aaaagaacat tatcagcact
    13141 tgactgagcg cagttttttt gatgaactga tcgattacat gacttccggt cctgtcgttt
    13201 atcttgtgct ggtcggcgag gaagtgatcg atatcgtccg taagatggtt ggagcaacaa
    13261 aagctgccga tgctgttcca ggtacgatcc gaggagacta cgcgctgccg gggactgaaa
    13321 acatcattca cgcttctgat tcccgtgatg ctgcggtaaa agagatcgcc cgctttttcc
    13381 cgccattcga tataaacaaa ccgtctgaaa aagcggcgaa gtgatacaaa ttctaaactg
    13441 ctggtctagt tgctgggttc agcaatggcc agcagttttt tctctaagga cctccgttga
    13501 ttctttccta acatatattc gagcactgtg aagaattctt ggtagtcttt cgtcttttct
    13561 tcccctaaag cagcagaaat caatctttga taataatctg ccatcaatgt ttcatagatc
    13621 actgtacttc tttctttatg tgctaagctc cgtttcatat cttcattcac actacgtgcc
    13681 tttttcaacc gaccattttc tatataaaac gaagtcaggt tcattaaaaa agtaaagaga
    13741 tactgctcat gattgttcaa ctgtgctaat cgtcgcattc tttttttgat tttcaaataa
    13801 ttgttctcaa tgaaccgatc actgaatatc caaggaaaat cagctagaat agatagttca
    13861 aattgaccat agtctggtat ctgcatcaag tattcttcaa tgattcggca ttcataacgt
    13921 aaaaattcaa attctgtctc agatacttct gttttttcga ccatgtgaag attcaaaacc
    13981 aaataataca gatcttctgt tttcttaaaa tgcctacggt tattgctgat ctctttataa
    14041 ggatcaaagc tgtcttcttg tacccgatct agcattcgtc tttttttctt taactttatt
    14101 ggttcatcca gctggtttcg catccattca tattctgcaa gtgtcatgtt cattcgctct
    14161 aaaaatgatc tcaatgttac aaatgacatt tttttcactg aatttgtctc aatcccgact
    14221 agagaagtct taaaacagat tccttccgct aattgggact gtgtcaactt cttgttgact
    14281 cgaagtttct tgatcaattc accatcatac ataatagttc tcctttttaa tttaaatttt
    14341 tccacattca ataaatagat acgttaaaaa ggaaaaatac acgaaaaaaa taaaaaaatg
    14401 tttttttcat aaaaaatacc aaccttcaag cattagaatg ttgtctaact actgaaggtt
    14461 ggtttgattg tttattgatt atttttttgc tgttcaacgt ctgttcttgt tactggatat
    14521 aagttggttg ttgttttatt accatttttt gaaaagacac ttgtcgaatg ttcgcctttt
    14581 tgcttttcga ttgcttcatt acgttccgct tcatttttat agtcatattt tttcggatca
    14641 atcgtctcaa atccgtttgg cacataaaac cgcaagagat tttcctgatt cagcttatct
    14701 gaaagactta acgcagtctt gactttttcc tgatctttgg agatttcctc atcgattcct
    14761 gctgctttcg tatcgatgat ttctcctgtc tgattattgt aagcttttcc tcctaggacg
    14821 gtgtatttgg gtgtcacaaa gttcccattt cgaaaagcaa ctacttgatc atgctgagaa
    14881 gataataagt ctgtaccaaa atgaagataa tctttatcat cgattcccaa tagatgtagt
    14941 aatgtcggca aaacatcgat ttctccaccg tattcatgat tgactgtccc tttagtataa
    15001 ccaggcatat ggatcatcaa tggcactcgt tgcatctgtg cgttgtcaaa ttcatcccat
    15061 gtatctggat ctttaccaag agcgcttgct aaatctttat tatctgtatt cgagatccca
    15121 aaatgatccc cgtaaatcac aaagattgaa ttttggtaaa taccagattt tttcaaatac
    15181 gtaaaaaatt gttccaatga ttgatccaga taatgtgccg ttcgaacata attatctact
    15241 gtgctattgc cagtattcaa agatggaaag tcgaaattct tgtcatccgt ataataaggc
    15301 gtgtggttgg ttacggaaag aaatttcgtg taaaacggct gttgtagatg ctcaagatat
    15361 tgggcagatt ctctgaataa atctttatct aatatcccat aacctaacgt ttcgtcggat
    15421 tcatcaaaat aagatcgatc aaagaaattg tcataaccta agtttttata tacatgatct
    15481 ctattccaaa aactagctac attcccatgg aaaacagcgc ttgtatagcc ttgcttttgt
    15541 ccaaggatcg ctggtgcggc ttgaaagaca ttgtctgacc caagttctgt gaataatgag
    15601 ccttgaggta aaccaaatgt gccagtttcc aacatatttt cagcatcact tgtctttccc
    15661 tgtcctactt catgaaagaa attgtcaaag ctgatcgttg cttgattctg gaaaatacta
    15721 tttaaaaacg gtgtgacttc ttgtccgtct accttcatat taatcaaaaa ctgctggaaa
    15781 ctttctaaat ggagaacgat cacatttttc cctttagcga tcccaaatgt ttcgggattt
    15841 ggctctgcat aatgtttctt tgtataatct aaaacttcat caatcccatt actgcttgca
    15901 tgcgcgcgaa cactgctagt cattcctgtt ttgattccgt cataaatagt atacgcatcg
    15961 attcctagat atttgacaat atatgaacgg tcaaatgttc gctgcagcaa ttgtggacgg
    16021 ttggcttcgc tcagcgctag atttcctgaa aaaataagcc ctgaaagaca ggtaacagcg
    16081 atagccatcg gtttgccgac agggttgctt ttgataggta ttttcttgac cttcaaccaa
    16141 accagtagcc caatgaacac agcgatatcc agccagtaga tgacatcatg gggcttcatc
    16201 aaagaaaagg agcttcctga caatccttgc gacactttcg aaaagcctaa tacagatttg
    16261 actgtaatga aatctgtgaa ttcccgataa aaaataatat tcaagtacaa caaaagcgta
    16321 tttaaaatat caatgatcaa aagaatagct aacgctggct ttggcttttt aatatacaaa
    16381 gaaaggccaa aaaacaataa ggtagtcgca attgggttga tccacaagat aaagtactgg
    16441 atcgtacctt ccacacctaa tgaaaaatct gcatagtagg caatgatcgt cttcagccag
    16501 aaaaagaaaa cagctgtcaa aacgattgct aaacgatttt ggcacaattt tttaagttgt
    16561 gtcacgtata tcacttctct ctattcacat tttttcttat tttgctcctg aaaaatgaaa
    16621 atttcctaaa aaagttgtta tcttaagatt tttttattct ttctaaagat tattccgtcc
    16681 acttttcaca gcataaaacc gctgcgatgt aagcgattaa actaatgaag agataaaagt
    16741 cttgtggtga ccgcttcact ccacggtatt caaaagccag tttctcggta ttcgtacaaa
    16801 aaaagaggct gtgacaaaat tcttgtcgca gctccttatt tcttgatgca ggacagcttt
    16861 ttcacaactt cttccttaaa cggtgttctc ctgccaactc ctcggccaca agtcacaata
    16921 tttataaaac atgagaagct gttttttgaa attgttcctt gtggctcaga gttaaacggc
    16981 aggctcaaga cctcttgata cacggtgttc aatcagctga ccctcggtat tagattatgt
    17041 caggacgcta attttacaac ctcttggtaa acggtgttca aaagcttgca tctgcggtaa
    17101 taagcccaac caaacgaaaa atataaggag ctattttttg aaattgctcc ttgtggctca
    17161 gagttaaacg gcaggctcaa gacctcttga tacacggtgt tcaatcagct gaccttcggt
    17221 attagctcaa aaaacggcaa aatatgagga actattttgc cgtttgtttc tctaatactc
    17281 aggtcaaaac gctgatttca caacctctgt ttttcattgt ccataggatt gatattgtcc
    17341 ttgtgatgac tcattcgtaa taattgaggc ttgattttct tcacttcccg ttatcgtttg
    17401 tccactgcca gaatctgaag gacttgttcc tgtcgactcg tttttttgag aagagacagt
    17461 tgttttcttg acttgttcat ttgtcttttt ccattccgct aaacgatcgg cgaccacttt
    17521 tttgtctact ttttcagaat aagcggtgat cacttttcca tttttcaaac gggctacttt
    17581 gtcgccatca tgagtcaaaa gcaaaacggt caaatgcttg tctttaaatt gatattgtct
    17641 gagagaaatc gcactaagta cgtcatcgat attaataaat tgattttctt cttttccttt
    17701 tggcaaataa atgtccaatt cgcctttttc gtaattggca gttattttat actccgccca
    17761 ccaataagtt gagaattctt ttttgaaatt atctactgat tctttgcttg ctacgatttt
    17821 ttctgtttct ttctgagcaa gatctgctgt catttttcct gaaaatgctg cggataaaat
    17881 aagcagtaag aaaaaaccga ataaccaaaa gagaagaaac ggtctttttt tcctcatgct
    17941 ttcctcca
//
LOCUS       DAYYCY010000044        17421 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000044 DAYYCY010000000
VERSION     DAYYCY010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.044"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..1602)
                     /locus_tag="ACHAZB_001185"
                     /pseudo
     CDS             complement(1..1602)
                     /locus_tag="ACHAZB_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1925..2404)
                     /gene="rlmH"
                     /locus_tag="ACHAZB_001186"
     CDS             complement(1925..2404)
                     /gene="rlmH"
                     /locus_tag="ACHAZB_001186"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFM8613874.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            2832..4121
                     /locus_tag="ACHAZB_001187"
     CDS             2832..4121
                     /locus_tag="ACHAZB_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HFM8613875.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(4160..4765)
                     /gene="ytpR"
                     /locus_tag="ACHAZB_001188"
     CDS             complement(4160..4765)
                     /gene="ytpR"
                     /locus_tag="ACHAZB_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtpR family tRNA-binding protein"
                     /protein_id="HFM8613876.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(4970..5434)
                     /locus_tag="ACHAZB_001189"
     CDS             complement(4970..5434)
                     /locus_tag="ACHAZB_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFM8613877.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(5456..5776)
                     /locus_tag="ACHAZB_001190"
     CDS             complement(5456..5776)
                     /locus_tag="ACHAZB_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFM8613878.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            complement(5920..6999)
                     /gene="pepA"
                     /locus_tag="ACHAZB_001191"
     CDS             complement(5920..6999)
                     /gene="pepA"
                     /locus_tag="ACHAZB_001191"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HFM8613879.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            7199..7549
                     /locus_tag="ACHAZB_001192"
     CDS             7199..7549
                     /locus_tag="ACHAZB_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFM8613880.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            7772..8719
                     /locus_tag="ACHAZB_001193"
     CDS             7772..8719
                     /locus_tag="ACHAZB_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM8613881.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(8776..10194)
                     /gene="pepV"
                     /locus_tag="ACHAZB_001194"
     CDS             complement(8776..10194)
                     /gene="pepV"
                     /locus_tag="ACHAZB_001194"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HFM8613882.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            10343..11188
                     /locus_tag="ACHAZB_001195"
     CDS             10343..11188
                     /locus_tag="ACHAZB_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HFM8613883.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            11192..11632
                     /locus_tag="ACHAZB_001196"
     CDS             11192..11632
                     /locus_tag="ACHAZB_001196"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFM8613884.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            11682..11876
                     /locus_tag="ACHAZB_001197"
     CDS             11682..11876
                     /locus_tag="ACHAZB_001197"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8613885.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            12012..12698
                     /locus_tag="ACHAZB_001198"
                     /pseudo
     CDS             12012..12698
                     /locus_tag="ACHAZB_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(13106..13795)
                     /locus_tag="ACHAZB_001199"
     CDS             complement(13106..13795)
                     /locus_tag="ACHAZB_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8613886.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(13770..15899)
                     /locus_tag="ACHAZB_001200"
     CDS             complement(13770..15899)
                     /locus_tag="ACHAZB_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HFM8613887.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            complement(15947..17284)
                     /locus_tag="ACHAZB_001201"
     CDS             complement(15947..17284)
                     /locus_tag="ACHAZB_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HFM8613888.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
BASE COUNT         5246 a         3527 c         3201 g         5447 t
ORIGIN      
        1 ataagaaaac taattaaaag aaagaggtaa ctacataaac taattaaaaa agctaacttc
       61 atagttggag gataaatgga agaaacttca atgattgttt ctccttgtgt tccttcaatc
      121 gacactagtc ctattatcaa tctctctttt ttgtccattt acttttgcga ctgttcccca
      181 ataatcaaat acaggaagcg ttattttttg tcctttagat tcatgtactg tatagactat
      241 tttcgaatcc atcacttgat aaggcatagt atgccattcc ttgccaattc ttacttcatg
      301 attctgcaaa tgttctttgt attgtaatgc ttccgtgggt gcatagtcat aaagtcctcc
      361 gggaaggagt gtcgtgtaac gaataagatt ttcttgattc attaccaagt gatctctctg
      421 agtgacaact ttatgttcat agtttgcttt tgttgcagca gatacagcca acagtaataa
      481 aacaattgcc gttatattaa ctacttttct ttgcttagct gatgtgctct tcatgaaaac
      541 gaacgataaa atcaacgagc caaacagtac ttgaaagcct aagattcgcc aaggaaattg
      601 aatcatttga atgggtgtat tttgtaataa agaccatgga aaaaatttgg tggataagag
      661 ccaagagact accgcactaa ggtaaatcac tttttctttg cgattaaatc ctttaaaaaa
      721 gaagagtgga cagatcaaag aaataatacc aatgattcct accgtaaagg ttgtcaggtc
      781 attattcatc gcaccaatca aactatccga gacatttaat gcttggtgtt gtaaggtcgt
      841 tttatctggc tcattaatcg attgaaccat gatttgtcgg aacatcggat accaaaagta
      901 tgctgttaaa agaatcgtca ttcctactgc ctttgacaac tccacaatca accggatgct
      961 ttcttttttt cttgtttcac ttactacaaa gccgcctaag actagtaagc caacggctat
     1021 tacactcata aaagtggaaa gaacatgtgt ataaatgagt aagctcatac tgactgctaa
     1081 tggaattgca catggctttt tctcataaat cagtgcgtaa gcataataca taactatcgg
     1141 tataaccgta agtgcaatcg cttcagcaat agcagaacga tgataaatat caatcgtacg
     1201 gtacccataa aatgtatata aacagctaaa tataaaagcc gcatcgattc tttttaaaaa
     1261 ttttagtcca taatgaaaca tcagcatgat agtacaaaca ttcaatagcc aaacatataa
     1321 gatataacta acgattaaat tattacttat ccaataaaaa atgactgccg gaaaaaaggt
     1381 caagtaggga taaaaataat tcactccgct cccataatga ttaaacgtcg taaaattgat
     1441 tggaccagaa aaaattgaag ataacccttt gactctagca aaatggaagg caatatcccc
     1501 cgatgtagaa atagtatgaa catttctaaa ataattgatt gaataaagtc cagcaataat
     1561 tgctaggaga atggacaata ttattttttt ctttacttgc attacttaat tatatactcc
     1621 tttcatggat tgctttatct agtataactt aaattttaaa taaatcgtta tttattttta
     1681 ctctcactta aaaaagcact aaaattataa gagagggagg taatggtctg aacaccaaga
     1741 attgttccaa catcgaattg cttaactaat gagttcaaag cttaattatg tggctcttat
     1801 atttaccgag cgtggattgc gatctcttta gcgaaacaaa catctttcat aaaaaaaaca
     1861 gccatatccg ctttcttgga tatgactatt tactactact ttactatctt tcattcttct
     1921 tgtctcactt atgataaggc gcccccgcat tgatccggaa ggcacggtag atttgctcga
     1981 caaggatcag acgcatcaat tgatgtggat acgtcatttt tccaaaagag atctgagcat
     2041 tgcttctttt catgacggct tcgctgagtc ctaaagagcc gccgataaca aaaacaaggt
     2101 ggctttttcc ttggatacct aattggtcga tttgtttagc aaaagattca cttgtaggat
     2161 ttttcccttc aattgctaag gcatagacgt attcttgttc tttgattttc gctaagatcc
     2221 gttccccttc tttttctttt acttgtctca tttgcgcttc gcttaaattc tccggggctt
     2281 tttcgtcagg aacttcgatc aattcgattt ttgcataggc attcagccgt ttgacatact
     2341 cattgatacc ttggatcagg tatttttctt ttaattttcc tactgagata attttgatat
     2401 tcatgttttc tctcctttgg tcttcatgct cactttgttt attataatca attttttcgt
     2461 ctatatgttt attttggcta tttttgttaa aaagatttcc ccaacagtta tgcacaacat
     2521 ccacaggttt atccacagaa aacagtgtta ttttacctta tttcatcaca ttcattaggc
     2581 aaacatacgt tttataagaa tttatccact ttatacacag gctgtggata tgttttatcc
     2641 cttatttttc caatcctatc cgcgtagttt tacacagtat ctgtggaaaa agaaaatcaa
     2701 atattttcat ctgaaaacaa tgatttttcc taaagttttt cataaaaagt tcacagtctc
     2761 aaagtagact aaagtcaaca aataagacta tactaacagt agataaatga atgtaattgg
     2821 aggaggaaca catggataga aaaaatgtga caccaaagat gaaaaaaagt aaaaataata
     2881 gcctctggcg taagttaggt ctggggcttg ttggagggat cgttggagga ctactgacag
     2941 ctggcatttt ttatgcagcg atgggcactg gcaacaatag ttctactgct acaagcggca
     3001 atcagaattc tgccggtgaa acagttgtgg aaaatgtcaa agtcaatgta gattctgata
     3061 tcaccaaagc ggtagataaa gttcaagacg ctgtcgtttc tgttatcaac ttacaaagtc
     3121 aaaaccaatc aagcggtttt ggcggactat tcgggcagca agaagaaagt agcagctcag
     3181 atgatagtaa tttggaagct tatagtgaag gaagcggcgt gatctacaaa aaagacggga
     3241 atactgctta tgtagtcacg aataatcacg tagtagatgg ccagcaaggt cttgaagtct
     3301 tgatgaaaga cggtacgaag gtaaaagctg aattagttgg aacagatgct tattcagatt
     3361 tagcggtgtt gaagatcaat tcagacaaag tcgaaacagt tgcctctttt ggtgactcaa
     3421 gtgctctgaa ggttggtgag cctgcgattg cgattggttc cccactagga tctgaatatg
     3481 caaattctgt cacttcagga atcatttctt ccttgaaccg acaagtgaca agtacgaatg
     3541 aatcgaacga aacagtcaat atcaatgcga tccaaacaga tgcagcaatc aaccctggaa
     3601 attctggtgg tcctttagtt aatatcgaag gacaagttat cggtatcaac tcaagtaaga
     3661 tcgcaagtac ttctgaatct acttctaatg taagcgtaga aggaatggga tttgctattc
     3721 caagtaacga tgtagtgaat atcatcaatc aactggaaaa agacggaaaa gtgacacgtc
     3781 cagctctagg tatcacaatg gtcgacttat ctgccgtttc tactcagcaa caagaacaaa
     3841 tcttgaagat tccttcatct gtaactaacg gtgtcatcgt gacaaatgtc caaacagcta
     3901 caccggctga aaaagctgga ttgaaacaat acgacgtcat tactaagatc gatgacaaag
     3961 aagtttcttc tggtgtagaa cttcaatctg ctttgtataa gaaaaaagtg ggagattctg
     4021 tcaaaatcac ttactatgaa ggctcgaaag aaaaaacaac tactgttgaa ttgacgatcg
     4081 atcaatcggc attaaaacaa aatcaaaata gtgaaaatta ataaaaaaga ggttgtgaca
     4141 aagacttgtc acaacctctt tattttggaa atgcttctcc gactacggca tcctcaggca
     4201 atactaagat tccttttttt gctggagcat ttggcaaatg caattcacga gcggaacaga
     4261 tcattccata gctatctacc cctcgcaaag ttcctggcca gatcatcatt ccatctggca
     4321 tcatagctcc tggttttgca acgacaactt tttgtccagc ttcgatattg ggtgcaccgc
     4381 aaacgatttg caatactgta ccattgtcta cttctgtctc agtaatcgat aaatggtcag
     4441 aatcaggatg tgggacgcat gtttttacgt acccgacaac gatcttcggt tcatgatcag
     4501 cttcaagctg ttcttcgaat cctgcttctt tgatcaatcg attcaatttc gctaattcct
     4561 catctgttaa gcttacttgg ccgttccctt cgattgttag atgatcggag atatggaaaa
     4621 agttccaagc aacaacttga ccttgttcat ctttgatccg agcaacattc catttgcgtt
     4681 caacagtgtt ttctgttcct ttatcgtcgg ctataataac catcaaggtg tctccgacgt
     4741 gtgctttgtt ataagcaaaa atcatgttct ttcctccatt atcctttgct gagatttatc
     4801 ttaacaaaaa attgcattca ctgcatgaaa tttttttcat gctttatttc cgaagaagcc
     4861 tcttctggaa caccatttat tcgtaacaag ggactgtgac aagacttttg tcacagtccc
     4921 tttactgttt tagttaattg attaaaaagg tcgtacaagt tccacttgct taattacttg
     4981 gctgttcttc ttcgtcagaa acgatcaaga catcacaagg tgaatgacgt atcacgtagc
     5041 tggctacact tcctgtcata aagcgttcga ctgcatttag acctgactgc ccaaccatga
     5101 ttagatcgat ctcatatttt tttggcaatt tgactgccat ggattcacga acagaaccat
     5161 atgcaataat tccttctata tttgtgtaat caacagattt cccatattct ttacattctt
     5221 cgatcatttc cttggctgtt tcagtttgct gatcgactag attttgatca ataggtgcat
     5281 agcccataaa gttataaaat tgctgatcga ttacgtttgc tacatatact ttcccattat
     5341 tacggcgagc cacttctacc gcttttttga aagcagcaag tgcttgttca ctaccgtcga
     5401 tacctactaa aatattctta tattcttgat tcatcttgac ccctcctaac agatgtcagc
     5461 cttttgttac actttctagg aacgattcga tttcctgttt tgttttacga tctttattca
     5521 ctagacgacc taattctttt ccgtcttgga tcacgacaaa acttgggata ccaaaaatgt
     5581 tccattcagc ggctacatct atgtacttgt cacggtctac ttcaataaat tgccaatcag
     5641 aaaagtcatt ttcaatttca ggcatttgcg gtttgataaa acggcagtct ccgcaccagc
     5701 cggcagtaaa gaaaaataca ctctttccag tttctacgta gcctgctaat tcttctaaac
     5761 tatttgggat aatcattctt tgtcctctac tttctgttta tgtatttttc aattaaatta
     5821 tacacctttc tttcgaacta acaaacattg atcctctttt tataaaaaaa atctggcaaa
     5881 aagcaatctt ttgccagatt ttatagtttc actatcgcat tattttccgt atacgatcgt
     5941 atttactgtt gatttatcca aacctttcag tgtttgaatc aacatttctc gtgcagcttc
     6001 aaaatcgcga atactgaaca tcgtttgatg cgtatggata taacgtccca ctacgccaat
     6061 cactgtactt gggattcctt cgttttgtgt atgagcagca cctgcatctg taccgccttt
     6121 tgaaacaaag tattgataag ggatattgtt tgtttcagcg atatccagca agtactcgcg
     6181 caaacgagga agcatgatca aacctggatc ttggatacgc atcaatgtac cttctcctaa
     6241 atgtccgaat gtgccatttt tcgtcactgt atcatctgct gcagagcagt ctactgcaaa
     6301 gaacagatct ggcttgaatt tattgacaga agctttagaa ccacgcaagc ctacttcttc
     6361 ctggacattc gcgcctgcaa tcaatgtatg acctaatgtt tcattttgca acgcttctaa
     6421 agcttctaat accattgtac agccatagcg gttgtcccat gatttactga taatattctt
     6481 tccgttggct gttttgatcg tttcagcgta aggaacgatt gtgtctcctg gaaggacacc
     6541 gtaactcatt gcctcttctc ttgattcaaa cccagcatcg aacaaaatat cggttacttc
     6601 tactgatttt tgtccgcttg ttcctcgcaa taaatgtggg ggaacggaag aagaaataca
     6661 tggataattc ttaccagtag aggttttaag tgtaaaacgc tgagcagaaa caacgtaagg
     6721 attccagccg cctagaggta cgactttaaa aagcccgttt tcttggattt gtgtcagcat
     6781 gaatccgact tcatccatat gtgctgcgac cattacacgg ggtgcatccg cttcttcatg
     6841 atggcgcaga ccaaaaatgc cgcccaaacc atctaactgt aactcatcta ccagcggttc
     6901 catatgactt tccatataag cgcgaacatc ttgttcaaaa ccgcttgttc cttgtaattc
     6961 tgttaactct ttgattcgtt gaaatgtttt ttcttccata tgctattcgt ctaaatttca
     7021 gactaaactc ctctcagact tatattgatc tgcggataaa atatgatttt ttctgcagac
     7081 cgatcgtatt tattatagct tattttttct ggtagcggtt atttatttca aagaaaatat
     7141 gagagtgtgg taaaataagc acaaggagta acaagtgaga agaaggaggt atgtccacat
     7201 gtatgaagaa agcgaactaa gctattttaa aggcgggtta gcagttggaa tcggtctagg
     7261 attattgagc ggggtagctt caacgatttg gtatcatcgc aaaaaagtat tgtcagcaga
     7321 ccaagtactt gaaaatatca agtcatcttt tctaaaagaa ggtgcaatcg aaggttcttg
     7381 gatctctttt gaaaaggaac ctattaggaa attcgccgtt cggtcgagtg cttatcgggg
     7441 aggtctttct cgaatagaag atggcgaaac tgtttattat gaatttttag cagatgctca
     7501 tacaggaacc gtattagaaa tttcaagaaa aaaaggtgca acagattaat acgtcaaaaa
     7561 agtgagagaa cgaatacgca cccactattc aaaagagagg tatcatgtcc ttcttttgta
     7621 gtgggtggtt ttttagatag agctttccaa acatatattc cagtattttc tttttaaata
     7681 aatgatattt agcgggaaaa actagcattt cctttgatta tgaggtattt ttaatttagc
     7741 ttttgttttc tatagttaag gagagtgaaa aatggaacaa caagaaaaaa ttgctgttct
     7801 gattccttgt tacaatgagg aagcaacaat ttcaacagtc attgcagact ttaaacgaga
     7861 attaccagaa gcagatattt atgtctatga taataattcg accgatcgaa catacgaatt
     7921 ggccgttcaa ggtggagcaa tcgtaaaaaa agaacctcgt caaggaaaag gaaatgtgat
     7981 ccgccaaatg ttctttgata tcgatgccga ctattattta atggtagatg gcgatgatac
     8041 ttatcctgca gaagctgtac atggtttgct cgagaaatta cgttctggtg aagcagatat
     8101 ggtcattggt gatcgcctat caaatggaac ctactttgat gagaataaga gacctttcca
     8161 cgattttgga aataaccttg ttagaaatac aattactaga atgtataaaa caaaaattct
     8221 tgatgtcatg accggctatc gaggattcaa tcgtatcttt gtcaaaagtt ttcctattat
     8281 gagttctggt tttcaaatcg aaacggaatt gacgatccat gctttagata aaaaattcaa
     8341 gttcgttgaa atcccaatcg attatcgtga tcgtcctgaa ggcagcgaat caaaattgaa
     8401 tacattctct gacggattca aagtgatcat gatgattatt aaaatgttta aagattataa
     8461 gcctttgctt ttctttggga tttggaccat cttcttcttt ttatttggat tggtcacggg
     8521 gattcctgtg attcgcgaat atatgctgac aagctttatt acacgcatcc cttctgcgat
     8581 tttaagtact ggattgatga ctttagcact tctttcatta gtaacagggt tgattttgga
     8641 tacagtcgtc acgaacgcta agaaagaata tgaattgaat ctatatcatg tttatcatga
     8701 ataccacagt agaaagtaaa aaaaggatca agactgcaaa aataatttgc aattcttgat
     8761 cctcttttta attaattatt tcgccaaacg atagatggca tctgcataga ttgctgttgc
     8821 tctgaacaag tcatcaagac tcataaattc gtttgcttga tgcattgtat cagtatatcc
     8881 tgggaacatc gctccataag caacgccacg tttcaacaaa cgtccgtaag ttccgccacc
     8941 gatgatttgt tcgtagcctt tttctccagt gtggtcttca tagacttgca ataatgtagc
     9001 caccaatgga tcatccattg gtacgtaatg tggttccatt acgcgagcac ctcgagtaac
     9061 tgttgcgcct tcttgcccca ctgtttgttc gatgtcactt tgcaatccgt ctactgttac
     9121 accttttgga aaacggaaat tcatattgat gaagttcgct tcctctgtac cattttcttc
     9181 aaaagcaaat agtccagcat tcattgtcaa tttgcccatt ttttcatctt cataagcgac
     9241 tcctagtttt tctccataaa aatcttcatg gacataagca gctgctacat ggataaattg
     9301 tttagcagat cctaggaatt catagttatc taagaacaat gccaagaaag aacctgcatt
     9361 gataccagat tgtgggcttg caccatgtgc gcctttacca actaattcga tttttacata
     9421 cgtattgtcc gcttcaatcg ttccgcttac tggatttgct tcgatgaatt ggtaaaatgc
     9481 ttcttccatc gcgatagctg catctgcact tggtacttga agtgctgcag ttgctgttcc
     9541 tggcaccata ttttcgcgaa gtccggaaac aaaagatttc aagacataat ctcctgcatt
     9601 gtcgcctttg aagcgtaaag ctaaagaaac atttcctttt tcaccattga tgattgggaa
     9661 ttcagcatct ggtgagaaac caaaatctgg ttcttcttca tgttcgaaat aataagccat
     9721 atcgccccag ccgctttctt catcgctgcc gactacgaag cgaacttttt ttgatagttt
     9781 caagtcaagt tctttaatga tcttcatcgc atagtaagct gccatacttg gacctttgtc
     9841 atcgcttgat ccgcgtgcaa agatctttcc atccttgatt actggttcat aaggatctgt
     9901 gtcccagcca tctcctgcag gaacgacatc catgtgtcca aatatcccta gcgtttcgtc
     9961 tccttcgcca agatcaatat gccctgcata gttatcgaca ttttttactt taaatccatc
    10021 acgttcgcca taagctaaca tatgtttcaa tgcgtcgcga ggtccaggtc caaatggtgc
    10081 atctggtgtt acttttgagt catcgcgctc gctgttgaca cgtaaaagat ttttcaagtc
    10141 ttcaagcaag tcttctttac gtgcttctac ttctttttgc caatcaattg tcataaagtt
    10201 aatcctccac ttatttaagt cttcgtgttc atgataccac aaattacaga aaaaaatagt
    10261 tcaaaaaatc tgacaattcc ttttcttatc tgtttttctc taaaaaaaat atacgctata
    10321 atgagtaaaa ggaggttttt agatgctgat aaacgaacaa ctagtgaaaa gagtcatcac
    10381 acctcgtcca aaaatttcac ataaaggaac atttggacgt atcgttttga ttggcggcaa
    10441 tgaacaatat ggcggagcaa tcattatgag tgctttagct gccgtacaca gcggagcggg
    10501 tctcacgact gtagtaactg atgaaaaaaa tcggagtgct cttcacagtc atctgccaga
    10561 agcaatgatt gtagtttgga cggatactga ccagtttctt tccgtaataa agcaagcaga
    10621 tgtactcttg attggtcccg gattgggtac ttcttctaag agtttggaac gtttcaaaca
    10681 agtactagaa aaccaatcaa aagaacaatg gctggtgatt gatggctctg ctttaagttt
    10741 acttgctgct aatcatcttt tgctacccta tcccgaacaa actgtactta cacctcatca
    10801 aatggagtgg caacggttga gttctctacc gatacaagaa caacgggata aagaaaacaa
    10861 aaagaagcaa caggaaatcg gtgctgtgct cgttttgaaa agtcatgaga caaaaattta
    10921 tggtcaccag cacttctaca ccaatccttt aggttctgct gctatggcaa cgggcggcat
    10981 gggtgacact ttagctggga tgatcacagg atttttagcc cagtttcctc aaaatgacca
    11041 aacgattgca gcagctattt atcttcatag tttcattgga gaccagctgg caaaagaaca
    11101 atatattgtt ttgccgacac aaatcagcga agcacttccg aaatggatga attatttttg
    11161 taaaaaatca atggaaaacg aggcatgagt catggagaaa tttacaggtt atgtgcgaga
    11221 acctttttat tatgaaacag atcaaatggg gattattcat cacagcaatt atatccgctg
    11281 gtttgaagag gctcgggtag ccttattggt acatctaggg tttccctata aaaaaatcga
    11341 agaatctggg atcatcatcc ccgttctgga agtaaattgc agctataaag aaatgatcca
    11401 ttacggagaa aaagtgagca tccatccaac tatccaaaag tatacgggga cacggttgga
    11461 ttttagctac gaaattgtgg gaactgaaga caaaaaatta aaaactactg gcagtagtaa
    11521 acactgtttt ttactttctg attcgcaacg attagtgaag ctgagcaaag tcaatccgga
    11581 gttagatcag ttattccaag cgtacgcttc tttggatgat gtaaattctt aataaagacc
    11641 gtctaagcat ttttcttgta ttgtgtcgag tagttcgtat aatgaaaaga aaaaagcagg
    11701 tgatccccat gtatgtcgtt aaagtaatgc acggttacat cgataaaact ggctgtcgca
    11761 ctcgggaaaa aaacttagac aatctgctca ttttcaaaga caaaaaagaa tccgaagctt
    11821 ttgctaaaag aatcggcggt cgtgtcaaac cgatccaaga ggtacgacca gattgattca
    11881 atatttttct tttcgaggct ctaataggca tgcctatcag ggcttttttt gcagactgtg
    11941 tttttatgcc cacacgattt gtaagcgatt tatgttataa tagattatat ctgcatagaa
    12001 aggaaggtta aatggttatc aataatttgc aacagcaagc ttatgaatct atacgtaaac
    12061 aaatcattta ttccgagtta gcacctggaa gtaaaatctc cgataaagtg ttagaagaac
    12121 aactagctat cgggcgtaca ccgattcgag aagcactgat ccaactgcgc aatcaagagc
    12181 ttgtaaaaac cattccacag tccggcactt atgtttcaaa aatcgatatc cgatctgctt
    12241 cattagcccg ttatacgcgg gaaaagttgg aaaaaccgat cctgcaagaa tgcagtgcga
    12301 aaatgaccaa gtaagatcaa gctaaactag aaaaaatact agaacaaacc gatcaggcag
    12361 taatcgctca aaataaaaaa gctttttttg atttggatac tgattttcac cgaacttgtt
    12421 atgaaattgc tggaaaacga gaaatttggg attggctaga aagttatagt acgcacttga
    12481 accgtttcag atggttacgt ttgacgattt cagaattaga ctggggtcga gtattagatg
    12541 aacatcagac aatgcttcaa tcaatgattg atcataactt tgatgaagtc ggatttcttt
    12601 gcacgatgca tttacatatg attatcgagg agcaagaata cgtgattcat aattaccccg
    12661 attattttga agatattcag gacagaccca tcaaataaga ggttttggaa tcagcgtttt
    12721 gacctgagta ttagagaaag aaacggcaaa atagttcctc atattttgcc gttttttttg
    12781 agctaatacc gagggtcagc tgattgcaca ccgtttattg agaggttgtg aaaaagctgc
    12841 cctgcatcaa gtaataagaa cagccaagta aaaatagctt cttatgtttt taacttggct
    12901 ggacttatta ccgcagatgc aagcttttga acaccgtttt ccaagaggtc gtgagcctgc
    12961 cgttcatctc cgagtcacaa ggaacaattt caaaaaacag cttctcatgt ttgttgaata
    13021 ttttgacttg tgaccgagga gttgacagga gaacaccgtt tattcggaaa aaaaggactg
    13081 tgacacgact tttgtcacag tctctttatt tgtaatctaa atcatactcc gtacatcttt
    13141 tttctatttt ttttatttct tctaagatcc cttggctacg aatttcatta tatggccaaa
    13201 gtaagactgc ctctaataca taaggcgaaa aatcgctttg attcatctct tttgaaaaac
    13261 tcatcgctgc tatctcgctc aatgcacgaa tgtttgattg atgtgtataa aaaggcaacc
    13321 gccacagcac aattttttcg ctttgcgtat aaattccctt aacagaagct tcgatgtggt
    13381 gaaatagttc atgtccaata atcaaagctt gcgctgtttc tttagagatc gaagctagtc
    13441 ctgaacattt taatttttct aacggctccg tcatcaatcg aatcccttca tcttctgcat
    13501 aagttgcaaa agtgattcgt tcgtttattt gctctgattc acaggaaacg attggaacag
    13561 ccatttgttc acaaatttct cctacatcgg caaaagggta cttttttttg atgctacatg
    13621 ccgtttcgat accacaatcg attgattgtt gtataagaat tttctgcttg gacaaaggaa
    13681 tctttttcca aaataaatct cgccgaaact ggtagaatcc ccaaatttct tcggggatct
    13741 gttgtaattc gtttagtaac tcttctaact tatgtttgtt tgatatttcc ggcaacgacg
    13801 atcacttctt cctccggttc catagtagat acttccagat ccatcaaaag ctgtagttgt
    13861 tccagatccg tcgctgtaaa atcagagact cttgctccta agcagagata aaattctggt
    13921 cgggcaatca attcagtttg ataaactgcc atatcttccc aaagttgttc gacagctgtc
    13981 atataattgg ctgcagttgt tttcatacat gaagcatgtc cacgttggac cttgataaat
    14041 cctttttgat cgatgatccg aagacttcca gcattttttg aacgattctg ttcgccgcat
    14101 acataaaaga aaggtgtatt ttccaataag caaacttcat ttttatttaa tcgcatatcc
    14161 tctgcagcaa gctctagtgc ttcttctgtt gtgatctcct tggttagatc ggttgctttc
    14221 acttccgtag aaccagtagc gatagccgtt acttttgaag tctgtggatc gatttctacg
    14281 tggacttcga tcgattctgg tgtagcgcct gattcaatcg ctttattcat cgcttcattt
    14341 ttcaacgatc gtatatcttc tttacttggc gaagggatga tacgttctac aacatctcgg
    14401 accattgcaa gtgctacacc aatagaagaa atcacttcgg cgttttcagg aatcgaatat
    14461 ttcacaccca ttttattact aaagtaagta atcaatgagg cagcgccgcc ccctacacct
    14521 acaagagaaa tctgatcttt ctccaagtga tacttgtctg ccaattctaa aatcaccggt
    14581 tcaatcttag cataggattt ttccatgatc tgttctgcga tgtcttcaac cgtcgtatga
    14641 caataatcag ctaatgcttg gatggctttt cgtgcagatg ggacatttcc ataagaaaaa
    14701 tgttcttctt gcactaatcc aaggacattg gctgcacatg tatttgtaat agtcacttct
    14761 tctccgtctt ccatcactac cttgacgtaa tcagcagggt ctccttcttt aggagagaaa
    14821 aattcaacct taggaccttt gattttttct gtctccgtaa atactgcata atctagaccg
    14881 gcaatatgtg cagaacgtgg accgacatcg atgattcctg actgattagc ccgtaccatc
    14941 gaaccgcctg ccacacctag aacccgaaca tctaatgaag agatatatgt tgggtggcca
    15001 cctacaattg aataatcgat tgctggacgt ccatttttaa tgaccccgat attggttgtt
    15061 gttccgccta cttcaaaata aacaccatta gacgctctca agtacatcaa agagcccatg
    15121 actgatgctg ctggaccgga aagcatggtt aaaacaggtc gttttttcat ttcattgatt
    15181 tccattactc cgccgtctcc gcgcataatc atcaaagaaa cattgacccc tgcttctcgt
    15241 acggaatctt ctgtagaggt tgccgtatct agcatttttg gtaaaataga tgcattgatt
    15301 gctgctgtcc gagttcttct tgtcaatcca tataattttg tgatgtcaga agccatcgtt
    15361 gtagggatcg attttaccga tgctgcttca taaacaactc gttctggttc cccattatct
    15421 actccaaatg ccatagaaga aaccaagacc tccgctcttt ctctttctaa agaagaaatc
    15481 gtttctgaaa cacggtctac atttaagtgt ttgacaggta agaatgcatt gacaatctct
    15541 atttttttct tattccctaa atcgatatcg ttcaatctag tttgtcgttt tgctaaaaaa
    15601 ccttctaatc caccttttgc catcccgatt acgccgactt ttgctacgtc tccttcaatc
    15661 agtgcattag tcgcttgtgt ggtagaatgt gcaacaaaca caacatcttc tggactgata
    15721 ttattttcca gcaaacaatt ttgaaaagac tgaacgacac ctgcggctac tccacggaca
    15781 tcatcgtggg ttgttttcac tgaagatttt ccgatgattt cgtgcgtcgc attatcgatt
    15841 gcgactgctt tcgtatgtgt accgccaaca tcaattccca ttcgcacaaa acgtttcatt
    15901 ttcttttcct ccattcatct aatcgttttt atcgacgttt tgtttactag gcgttcccgt
    15961 acaataagaa cgtgatcagg cataagatcg ctccgatgat ccaaccagtt gggatggatt
    16021 tcttcatata ttctcgagaa gttacttttg tataacctaa tccccaagca acccaagatt
    16081 gtgtaatatc taaatgctga ggagcaatcg ttgtgattgc aaataacggg aacaaaaagg
    16141 ctggcgacat cgttggatta acagctaata cgacagctaa aatagctgag cctgaaccga
    16201 ctagattcat tggaccgcgg aaaaatccta gtggtgtcaa tatagcaaat acgattgcca
    16261 agactaatgg tgattgcggt atccaatctc caaaaatagc tgaaaaataa ggagaagcat
    16321 acgcagctga attattgaac atcgctaagg tcatcaagaa accaaccatt ggcgcaacat
    16381 ctactgcgcc atctgtaaat aatttcgcaa actgacgaca gatttcttta taacctccgc
    16441 gcaattttcc agtaaacagc aatgcccaga tacctgctag aataaaacct aagatgattg
    16501 gaagatctaa aagaacgacc cctaaaactg gtaataatac ggctaaccat gagatcattg
    16561 gtgcgtcgcc accttcagct ggtacgtttg ctgccattgc atagcgtttc tttttgttca
    16621 ttgatatatt agcgaccgtt agtaccacaa ctaagcttac aatcatccca atcataccaa
    16681 tttggaaata gtcattataa gtatagcttt cagccgctgg attgaaacca gcataaatcg
    16741 tttgatattg tttgaaattg acgatattag caaagattcc agccataatc gagcccataa
    16801 atgagaacag tgcaaccggt actgaaatac ctaatgacat caagattggc aacacaataa
    16861 ctgcaataga aatcactggt ccgattcctg tcatcgacat gaatataaca gcggttacaa
    16921 tacatagtaa gctcatcgtg attcttggct tgtcccctcc aagttcaact acctttcgaa
    16981 tcagggtagc tgcgattccg ctatctacta gaacacgccc gaaaaaggca ccaaagaaga
    17041 catttaccaa gatcgatttg gcgtactcag ctggccccgt cgtatagata tgcgtcaaca
    17101 catcaattac tccttgccct tccatcgccg ggtttggtga aaacgtgttt ccgactagcg
    17161 ctaatcccat ccatacagta gccatgatgg caaagccgac catcaagttg tagcctttta
    17221 cacagtacca gaccatgaaa aagaatgaga taactaacaa aataccaata ataatatcac
    17281 tcatgttatt cctccgtcca ttttgttttt ctgataagca aactaatata tcagtcaaac
    17341 tcattctaac ttatgtagtt agcgctttca ttaaagggtg accataaatg ttcagttttc
    17401 aattaaaaaa ccttaattat g
//
LOCUS       DAYYCY010000045        16968 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000045 DAYYCY010000000
VERSION     DAYYCY010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16968
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.045"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1152
                     /locus_tag="ACHAZB_001202"
     CDS             <1..1152
                     /locus_tag="ACHAZB_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HFM8613889.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSA
                     EAPKEKVETSGSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKED
                     IENFLAGGPSSAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPT
                     RKAIAKAMVNSKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALT
                     ATVKKYPVLNASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEI
                     NEKAKLAHDGKLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIV
                     NAEGEIVVGRVMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            1160..2566
                     /gene="lpdA"
                     /locus_tag="ACHAZB_001203"
     CDS             1160..2566
                     /gene="lpdA"
                     /locus_tag="ACHAZB_001203"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFM8613890.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            2728..3003
                     /locus_tag="ACHAZB_001204"
     CDS             2728..3003
                     /locus_tag="ACHAZB_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HFM8613891.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            3009..3776
                     /locus_tag="ACHAZB_001205"
     CDS             3009..3776
                     /locus_tag="ACHAZB_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFM8613892.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            3944..5773
                     /gene="typA"
                     /locus_tag="ACHAZB_001206"
     CDS             3944..5773
                     /gene="typA"
                     /locus_tag="ACHAZB_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HFM8613893.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            6034..7806
                     /gene="recQ"
                     /locus_tag="ACHAZB_001207"
     CDS             6034..7806
                     /gene="recQ"
                     /locus_tag="ACHAZB_001207"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HFM8613894.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            7920..8222
                     /locus_tag="ACHAZB_001208"
     CDS             7920..8222
                     /locus_tag="ACHAZB_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HFM8613895.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            8225..9388
                     /locus_tag="ACHAZB_001209"
     CDS             8225..9388
                     /locus_tag="ACHAZB_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFM8613896.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            9414..12842
                     /locus_tag="ACHAZB_001210"
     CDS             9414..12842
                     /locus_tag="ACHAZB_001210"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HFM8613897.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            12863..13987
                     /locus_tag="ACHAZB_001211"
     CDS             12863..13987
                     /locus_tag="ACHAZB_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP-associated domain-containing protein"
                     /protein_id="HFM8613898.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            13968..14414
                     /locus_tag="ACHAZB_001212"
     CDS             13968..14414
                     /locus_tag="ACHAZB_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFM8613899.1"
                     /translation="MIAVIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESA
                     KVCQLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQ
                     LQSLLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            14398..14736
                     /locus_tag="ACHAZB_001213"
     CDS             14398..14736
                     /locus_tag="ACHAZB_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HFM8613900.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            14855..15415
                     /gene="rsmD"
                     /locus_tag="ACHAZB_001214"
     CDS             14855..15415
                     /gene="rsmD"
                     /locus_tag="ACHAZB_001214"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HFM8613901.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            15412..15903
                     /gene="coaD"
                     /locus_tag="ACHAZB_001215"
     CDS             15412..15903
                     /gene="coaD"
                     /locus_tag="ACHAZB_001215"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFM8613902.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            15893..16936
                     /locus_tag="ACHAZB_001216"
     CDS             15893..16936
                     /locus_tag="ACHAZB_001216"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepM family pheromone-processing serine
                     protease"
                     /protein_id="HFM8613903.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
BASE COUNT         5749 a         2934 c         3747 g         4538 t
ORIGIN      
        1 ccgtcacctg tcacagggac agttaaaaac gtgattgttc cagaaggcac tgtagcaaat
       61 gttggggatg tattagtaga aatcgatgct cctggacaca atagtgcacc gtcaactagt
      121 gcgccatcag ctgaagcacc aaaagaaaaa gtggaaacat caggttcagc aagtgtcgtt
      181 gaagctgccg atccaaataa acgtgtattg gctatgccat ctgttcgtca atttgcccgt
      241 gaaaaagatg tagatatctc acaagtaact gcaactggaa aaggtggcag agtaacaaaa
      301 gaggacatcg aaaacttctt ggcaggtggt ccaagcagtg caccagcgaa atcagaagcg
      361 ccagaagcgg cagcaccgaa agaagcagct cctgcagcag aaagcaaacc agctgcacca
      421 gcaaaaccat tcaagtccaa cttaggagac ttggaagaac gtgtagcaat gacgccaaca
      481 cgtaaagcta tcgctaaagc aatggtcaac agtaagcata cagctccaca cgtgacattg
      541 catgatgaag tagaagtgtc taaactatgg gataatcgga aacgatttaa agaagttgct
      601 gctgcaaatg gtacgaaact tactttcttg ccatatgtcg tgaaagcttt aacagcgact
      661 gttaagaaat acccagtatt gaatgcatct atcgacgatg caaaccaaga aatcgtttat
      721 aaacattact acaatatcgg tattgcaact gatacggatc acggactata tgtaccaaat
      781 gtgaaagatg cagaccgcaa aggaatgttt gctattgcag acgaaatcaa tgagaaagca
      841 aaattagctc atgacggcaa actatctgca gaagatatgc gcaatggaac aattacgatc
      901 agcaatatcg gatctgtagg tggcggttgg tttacaccag tcatcaacta tccagaagta
      961 gctattttag gtgtaggaac gattgcacaa caaccaatcg taaatgctga aggcgaaatc
     1021 gttgtcggtc gtgtgatgaa actgtcatta agtttcgatc accgtatcgt agatggagca
     1081 actgcgcaac aagcaatgaa taatatcaaa cgtcttttag ctgatccaga gctattaatg
     1141 atggaaggat gataaacaaa tggtagttgg agattttgct gtagaattag atacagttgt
     1201 aattggtgcg ggacctggtg gctatgtagc tgccatccgc gctgctgaaa tgggacaaaa
     1261 agtagcaatc attgaaagag agtatattgg cggtgtttgt ttgaatgtcg gctgtattcc
     1321 ttcaaaagca ctgatagctg caggacacca ttatcaagag tcactagatt ctgaaatgtt
     1381 tggagtaact agtgaaaacg tcaaactaga ttttgcaaaa acacaagaat ggaaagaaaa
     1441 caaagttgtc catacgttga cttctggtgt tggtttccta ttaaaaaaac ataaggttga
     1501 aacaatcgaa ggagaagcgt tcttcgtaga tgatcacaca ctacgcgtca ttcatccaga
     1561 ttcagctcaa acttattcgt tcaataacgc aatcatcgca acaggttccc gtccaatcga
     1621 gattccagga tttaaattcg gaggacgtgt gttagattct actggcggat tagcattgaa
     1681 agaagtacct aagaaatttg tgatcatcgg tggcggtgtg attggtgccg aattgggcgg
     1741 agcgtacgca aacttaggag cagaagtaac gattcttgaa ggctcaccac aaattttacc
     1801 aacttacgaa aaagatctag taaaattagt agaagatgat ttcaagaaaa aaggcgtaac
     1861 agtcgtaacg aatgcaatgg cgaaagaagc agtcgataac ggtgacagcg tgacagtgaa
     1921 atatgcagtg gacgggaaag aagaatccgt tactgctgat tatgtgatgg tcactgtagg
     1981 tcgtcgtccg aatacagatg atatgggctt agaacaagcg ggtgtcgaag taggagaacg
     2041 aggattgatc acggtggata aacaaggacg tacgaacgta cctaacatct atgccatcgg
     2101 tgatatcgtt ccaggggcgg cattagcaca caaagccagt tatgaagcaa aaatcgctgc
     2161 tgaagcaatc tctggcaaaa aagtagctgt agattataaa gcaatgccgg cagtagcctt
     2221 cactgatcct gaattggctt ctgtaggtat gacgatcaaa gaagcaaaag atgctggtat
     2281 cgaagcaaaa gcatataaat tcccattttc agggaacggt cgggcattat cgcttggtaa
     2341 aacagaagga ttcatccgtc tagttacgac gatcgaagat aacgtgctta ttggcgcgca
     2401 aatcggtgga gtcggcgcaa gtgatatggt ctctgaatta gctttagcaa tcgaatcagg
     2461 aatgaatgct gaagatatcg ccttgacgat tcatcctcat ccatctcttg gcgaaatcgt
     2521 catggatgct tcggaattag cattaggttt accaatccat atttgatttg aataatacag
     2581 gcgaaacgca actgaaaaat cagttgcgtt ttgtttttta gaattatcaa taatattatt
     2641 atgtaaactt aaaaatatga caacttcttg gtttattacc acttcctatg ctacactgaa
     2701 gaggaatatg aaataggaga gttgaagatg caagaatatc agtatccact tgatttagat
     2761 tggacaacag acgaaatggt cattgtaatg aagatgtggg aagatctgga acgtgcgaat
     2821 gaaaaaggga taaacaatga agcttttttg aagacttatc agcagtttaa aactgttgtc
     2881 aaatccattg gtgaggaaaa acgattggga cgggaatttg aaaaagcttc cggttattct
     2941 ttatatcgta cagtgcagca agcaaaaaaa aatccaaaca aaactttgag aatgaacggg
     3001 tgagataaat ggaacagatg attgatcata tcaagtcctg gcttgaagaa gctggtgaga
     3061 aaatcaaatc ttctcaacaa acattaacaa tctcgcaaaa atcaaatcga acggatctag
     3121 taacaaatgt cgatcgaaac atccagaatt ttctaatcga aaaaatccat tcctttgatc
     3181 cagaggcgaa aatattggca gaagaagaag ggtgtaatca attatcggat ctcgatggaa
     3241 gagtctttat catcgatcct attgatggca ccttgaattt tgtgattgaa ggggaaaatt
     3301 tttgtattat gctcgctgtt tatgaatccg gcgttggaaa gcttggtttc atttatgacg
     3361 tgatgagaaa tgaacttttt tggggtggaa aaggaatagg cgtgtataaa aatgatgagc
     3421 cattgcctag accagcaaat aaatccttat cggaaggctt acttggtgtg aatagttatc
     3481 ttttcggtca taataaatat ggaatccgag aaattgcgga aagcagcatg gggacacgta
     3541 tgtacggatg cgctggtctt gaactagtgg caatcttgaa aggaaaccac attgcttata
     3601 tttcaaacct tagcccttgg gattatgcag cgggaaatgt cttattggaa gagtttggta
     3661 tggtaacaag cggtcttttt ggtactcctt tgacatttga aaaaagggaa tactatttag
     3721 cagggacttc ttgtgcatat gcagaaatcg cagacctttt gaaaaaagaa ggctgaaaaa
     3781 aaggtagaaa tcattcacaa aaatgataaa aacgagcaaa agattaattt tctcgaaaaa
     3841 atgaaaacat tttactatat ttcatgaaaa aagtctgcta taattagcag tcgagtaaaa
     3901 tttgccctgt cataaagaac caataaaaaa ggagttagtt acattgaatt atagaaacga
     3961 tatccgtaac gtcgcaatta ttgcccacgt tgaccacgga aaaacaacat tagttgatga
     4021 actattaaaa caatcagata cattagacgc acatacgcaa ttgcaagaac gtgcgatgga
     4081 ctcaaatgcg ctagaaaaag aacgtggtat cacgatttta gccaaaaaca cagctgtaga
     4141 ctataaaggt attcgcgtaa acatcatgga tactccagga cacgcggact tcggtggcga
     4201 agtagaacgg atcatgaaaa tggttgacgg cgttgtgttg gtcgtagatg cgtatgaagg
     4261 aacaatgcct caaacacgtt ttgtattgaa aaaagcactg gaacaacata tcacaccaat
     4321 cgttgtcgta aacaagattg acaaaccttc tgctcgtcct gagcatgtcg tagatgaagt
     4381 tcttgaacta ttcatcgaat taggagcaga tgatgatcag ttagactttc ctgtgattta
     4441 tgcatctgca ctaaatggta cgtcaagctt gtcagatgat ccagcagatc aagaaccaac
     4501 catggcacct atttttgata caattattga aaaaatccct gctcctgtcg ataacagcga
     4561 cgaaccatta caattccaag tttcattact tgactacaac gattatgtag gtcgtatcgg
     4621 aatcggacgt gtatttagag gaacaatcaa agtaggagac caagtagcat taattaaatt
     4681 agacggaaca gttaagaaat tccgtgtaac gaaattattt ggtttctttg gactaaaacg
     4741 tctagagatt caagaagcaa aagctggaga cttgattgct gtctcaggta tggaagatat
     4801 ctttgtcggt gaaacagtta ctcctgtgga tcatcaagat gcgttaccta tccttcacat
     4861 tgacgaacca acattgcaaa tgactttctt agttaataat tcaccatttg ccggtcgaga
     4921 aggtaaattc gtaaccgctc gtaagattga agaacgtttg atggcagaac ttcaaacaga
     4981 tgtatcctta cgagttgaac caacaaactc tccagatgca tggacagttt ctggtcgtgg
     5041 agaattgcat ttgtctatct tgattgaaaa catgcgccgt gagggatacg aattgcaagt
     5101 atctcgtcca gaagttattg aaaaagaaat cgacggtgta aaatgcgaac cgtttgaacg
     5161 cgtgcaaatc gatacaccag aagaatatat gggtagtgtt atcgagtcct tgagcttacg
     5221 taaaggtgaa atgcaagata tggtccatac aggaaacgga caaatccgtt tgacattctt
     5281 aacacctgcc cgcggtttga ttggttattc aactgaattc ctttcaatga cacgtggtta
     5341 cggaatcatg aaccatactt ttgaccaata tctgccaatg cttcctggtc aaattggcgg
     5401 acgtcaccaa ggggcgcttg tttcgatcga tacaggaaaa gcaacgactt attctatcat
     5461 gagtatcgaa gaacgcggta cagtcttcgt agaaccaggt acagaagtat atgaaggaat
     5521 gattatcggg gaaaacagcc gtgataatga tttgactgtc aacatcacga aagcaaaaca
     5581 aatgacaaac gtacggtctg ctactaaaga ccaaacatct gtcatcaaaa aaccaaaaca
     5641 attgacgttg gaagaatctt tggaattctt gaatgatgat gaatattgcg aagtaacacc
     5701 agaaagcatt cgcttgagaa aacaaatttt agagaaaaat gctcgtgaaa aagcatctaa
     5761 aaagaaaaaa taatctgtaa aaggatgaag agagaaacca gcttgttata aaaaatagct
     5821 ggtttctttt tttgtccaac tcttttctgt ggagaagtca tctttgtcaa tggacataaa
     5881 ttcagaaaat acgccgaaag acgtttaaaa gacttttaaa aaacttattt ctagagagtt
     5941 tttgtttcat aataagataa tgtttcacga aaggagatca acttgtcctt cgaagaaaat
     6001 cgaagtaaaa tggcaagaga aaggagcggg aagatgaacg acttattaaa acaatacttt
     6061 ggttatgatg agtttagacc tggtcaaaaa gaaattatcc agaaagtcat tgatcaggaa
     6121 aatgtattag gaatcatgcc taccggaagt gggaaatcaa tctgttacca gcttccagct
     6181 ttactattag atgggcttac tgtggttgtg tcccctttaa tctctttgat gaaggatcag
     6241 gtggacgcgg cgaatcaatt aggtataccc gccactttta tcaatagctc gttagatggt
     6301 tacgaaacag ctagacgctt ccaagaaatc gatagacagc agtaccgttt actttatatc
     6361 gctcctgaac gttttatcat gccggatttt atccaagcaa tgaagcgatg gaacgtccgt
     6421 atgatcgcta ttgacgaggc acattgtatc tctcagtggg ggcatgattt ccgcccaagt
     6481 tatttacaga tggcgaacca gctagatcaa ctgccaaatc gtcctgtaat tgttgctttg
     6541 acagctacag cgactgtgca agtagctgct gacataaaaa gacttttaaa aattccagaa
     6601 aacaaccata ttcagactgg ttttgaacga gaaaatctac gatttcaagt aattaaagat
     6661 caaaaaaaag aacaatatct aatcgaatat ttaaagatca acaaaaatca atctggtatc
     6721 atttatgcag caacgagaaa agaagtagac cgtctctatc atttgcttaa aaaattcgat
     6781 ttttccgtgg gtagatatca tggtggtcta aatgagaatg agcgaacaga aatgcaagaa
     6841 gcctttttat acgatcgctt gcagctgatt gtagcgacaa atgcatttgg tatgggtata
     6901 aataaaagca atgtccgttt tgttatccat tatcaaattc ctggctctct agaagcatac
     6961 tatcaggaag ctggtcgggc gggaagagat ggtctttcca gtgaagccat cctgcttttc
     7021 tctcctcaag atatccaagt ccaaaagttt tttatccagc agtcccaaag agaagaaggg
     7081 caaaagcaaa aagaatatga aaaattaaaa gcgatgacag aatacgtgta tattgagtcc
     7141 tgtcttcagc aatacatcct aaactatttt ggcgagacaa gcagtccgtg caaccgttgt
     7201 ggtaactgtt tagatgacag agaaatagta gaagtaacga cagaagcaca gatggttctt
     7261 tcttgcttaa aacgtatggg tgagaactat ggtaaacaaa tgctgatgaa agtcttagca
     7321 ggatcgaaag aacaaaagct gcaagcttta ggttttggac atttatctac ctatggactg
     7381 atgaaaaacc aatcgcaaaa agaaacgatg cagttaattg aatatttgat atcgaatggc
     7441 tatcttctga ccatcaatgg tgagtatcct gttttgaaag taacagaaag agggatacaa
     7501 gtattgaaag gacaagaatc tgtttatcga aaggaaccca agaaagtcca gcagctatcg
     7561 gatgaagaaa ccgatacact atttgaagtg ctgcgggaac tgcgaacgga tctagcatca
     7621 gaagcaggcg ttcccccgta tgtcgtattt tcagattcca cattgaaaga aatgagccga
     7681 atacgtccat caagcagatt ggagatgctt cagataaaag gagttggcca aagcaagctt
     7741 gataaatatg gcgaagcctt tttatctcgg ataaaaaatg cggactctaa cgttctggat
     7801 aagtagaaag aacgaatacg taaaaatgaa taaaaacaac tggttaaatc tgtttttttt
     7861 gctccttatg aaaatcaaat atgttataat taaggaatta gaatgggagg taagcacaga
     7921 tggattcttt gtcgaaaaaa cttgcgatcc aagtattaaa tgatgaagca gaacggatca
     7981 aacgattaat ccgaaatcaa aacaacagtc tctgtatctc acaatgcaaa gccttcgaag
     8041 aagtggtaga tacacagatg tacggctttt cccggcaagt cggttttgct gtccgtatag
     8101 gagtaatcgg aaagaacgag ggccagctga tgttaagtga gctagaacga gaattgaatc
     8161 agttatatag tgaggtttac caagaaattt atgataaaaa agaagttggc aaggaggaat
     8221 agacttgcct aagaaagtga aaaaaaggca tctattagac tatagcatct tgattcccta
     8281 tttaatttta tgtgtattgg gattgatcat ggtatatagt tctacgtcgt atctattatt
     8341 agaaaacgga caaaatccgt ccgcatcagt cattaaccag agtatttttt gggtgcttag
     8401 cttgattgct atcgcactac tttataaaat gaaaacagat gttttgaaaa accagcgttt
     8461 gatcatggca gccatcgcag ttttgacgat tttgctgctg attgttgtgt tttttggtaa
     8521 ggagatcaat ggggcgaaag gttggctgca aattgcagga ttctcgatcc aacctgccga
     8581 atacctaaaa atcatttcta tctggtacct gtctctgact ttatctaaaa gacaaaacag
     8641 tgtacaaaaa gattttcttg ggactgtgaa acgtccactt gcaatggtta tcggacttac
     8701 tgcattagtc gctattcttc ccgattttgg taatgcggca gttatctttt tgattatttt
     8761 ggttctgctt ttagccagcg gagtcaatta tgtgtatact ttgattgtag gagtaggagg
     8821 cttttgtcta agtactttta cgatctggct gatcaatatc acaaacggaa agatcttacc
     8881 cggacgttta cagtatatct ataatcgatt tgctatttac caaaatcctt tctcagacga
     8941 attaaataaa gggcatcaac tagtcaacgg ctactatgcc atgttcaacg gaggactatt
     9001 tggcagggga ttaggtaaca gtatccagaa aaaaggattt ttacaagaag cacaaaccga
     9061 ttttatttat gcaatcgtag tggaagaact gggagtgatt atggggattt tgatattagc
     9121 gttgctattc tttatgattg tgcgcattgt ccttgtaggt atccgttcaa aagatccgtt
     9181 caattcgctt ttgtgcattg ggatcggcgc aatgtttttg atccaagtat ttgtcaatct
     9241 tggaggaatt actggcgtta ttcctttgac agggatcacg ttcccatttt taagtcaagg
     9301 aggctccagt ttgctgatgc tttcgatttg tgtcggcttt gtcctcaaca tcagtgcgga
     9361 tgaaaaacgc aagagcttag gtttataata aaaaaagatg taggagtggt tcgatgaaaa
     9421 aagttttagt tgctaacagg ggtgaaatcg ctgtacgtgt atttcgtgct tgtacagaat
     9481 taggaattaa aactgttgga atctatgcag aagaagatga atattccgta caccgtttca
     9541 aagcggatga agcatattta gtaggacaag gaaaaaaacc gattgatgcg tatctagata
     9601 ttgaagggat catttctatc gcgaaagaat gcggagctga cgctattcat ccgggttacg
     9661 gtcttttgtc ggaaaacttg aattttgcgc aacggtgcga agaagaaggc atcatttttg
     9721 ttggacccaa actgcatcat ttagatattt ttggagataa aatcaaagca aaagctgcag
     9781 ctatcgaagc ggggattgct tcgattccag gcacagatgg accaatcgct tctatagacg
     9841 atgccttgga gtttgcgaaa caatacggtt atccgattat gatcaaggca gctctaggcg
     9901 gcggcggacg cggcatgcgt gtagctcacg atgaaaagag tgcaagagag ggctacgaac
     9961 gtgcgaaaag cgaagcaaaa gcagcctttg gtagtgatga agtgtatgtt gaaaaataca
    10021 tcgctaatcc taagcatatc gaagtacaga ttctcgggga tacgcatgga aacgtcatcc
    10081 atctttttga aagagattgt tctgttcagc gccgtcacca aaaagtagta gaagtagcgc
    10141 cttgtgtctc tatgaatgaa cagcaacgtc aaaaaatctg ccaggcagct gtccaattga
    10201 tgaaatatgt cgggtatgtc aacgcaggta cagtagaatt tcttgttgaa ggagatgatt
    10261 tttactttat cgaagtcaac cctcgcgtcc aagtggaaca tacgatcaca gaaatgatta
    10321 cagatattga tatcgtaaca acgcaactgt tgattgctca aggtttggat ctgcataaag
    10381 aaatcggatt gcctcagcaa gaaggaatta aattaaatgg ttcagccatt caatgccgaa
    10441 tcactacaga agacccgttg aataactttt taccagacac agggaaaatc gatacgtatc
    10501 gttcacctgg tggttttggc gtacgtctgg atgtcggaaa tgcttacgcg ggctatgtag
    10561 tgacaccgta ttttgattca ttgctagtaa aagtatgtac gcatggtgct acctttgaaa
    10621 cagccattca gaaaatggaa cgttgcttga aagaatttag aatccgtgga gtgaaaacaa
    10681 acattccatt tatgctcaat gtcatcacac atcctgaatt ccaatcagga aatgcaaaaa
    10741 caaccttcat tgatagtaca gcaacattat ttgaatttcc tcgactacga gaccgcggaa
    10801 acaaaacgat gaaatatatc ggtgaaatta ctgtgaatgg ttttccaggt atcgaaagtg
    10861 gcgaaaaacc attttatgaa gaaccacgta tgcctaaaga tctaatcaca agaactgatt
    10921 acgtaacggc taagaatgta ttagatgcga agggtgccga tgctttggtc gaatggatca
    10981 aaggacagga aaatttgttg ttgacggata cgacatttag agacgcccat caaagtctgc
    11041 tagcaactcg tgttcggaca aaggatttca agcagattgc acgtttgact ggagaaggtc
    11101 ttcctgaact cttttcaagt gagatgtggg ggggagcaac atttgatgtg gcttaccgct
    11161 ttttgaatga agatccttgg caacggttga gaaaaattcg ttctttaatg cctaatacgt
    11221 tgctgcaaat gcttttcaga ggttcaaatg ccgttgggta ttctaattat cctgacaatg
    11281 tattggtgga atttgtcaaa gaagcagcag cgcaaggaat cgatgtattt cgaatctttg
    11341 atagtttgaa ctggactcca caaatggaaa aaagtatcca agctgtacgg gatactggta
    11401 aaatcgcaga ggcagctatt tgctatactg gcgatatcaa cgatccaagt cgggcaaaat
    11461 acaatgttca gtattataaa gacatggcaa aggaactaga acaactgggt gcgcatatca
    11521 ttgcaatcaa agatatggct gggctgctta aaccgcaggc agcttatcgt ttgattagtg
    11581 aattgaaaga aacgacagat ctgccaatcc atctgcatac tcatgatacc agcgggaatg
    11641 gaatcatcac ttattctgct gcttcaaaag caggagtaga catcgttgac gtggcaatga
    11701 gtgcaatgag tgggaatact agtcagccta gcatgagcag cttgtattat gcactggtca
    11761 atggtccacg gctgccagaa atcacgattg aaaatgcaca gaaattaaac cattattggg
    11821 aagatgtgcg catgtattac aaaccgtttg aaaacggctt gaatgctcct gaaacagaag
    11881 tatatatgca tgaaatgcca ggaggacagt attcgaatct acagcaacag gcaaaagctg
    11941 tcggattagg tcatcgctgg gatgaaatca aacaaatgta tcatactgtc aacttgatgt
    12001 ttggcgatat cgttaaagta actccatcat ctaaagtagt tggagatatg gcactattta
    12061 tggtccaaaa tgatcttaca gaagaagata tttatgaaaa aggcgaaaca ctaagtttcc
    12121 ctgaatctgt tgtgactttc ttccaaggag aactagggca acccgttggc ggatttccta
    12181 aaaaattgca aaagattatt cttaaaggca gacctgcatt gaatgaacgt ccaggtcttt
    12241 tggctgaatc agtcgacttc aacgaagtaa aaaaagaatt ggcagaaaaa attggctatg
    12301 aaccaaaaca agacgaagta ttaagctatt tgatgtatcc gcaagtcttt ttggactatc
    12361 aaaaagcata taatcaattt ggcgatgtta cattactgga cacaccaaca tttttccaag
    12421 gtatacgttt gggtgaaaca atcaatgtac aaatcgaacg cggaaaaacg cttatcattc
    12481 gtttagatga aatcggagaa cccgatatcg aagggaatcg cgtactgttc ttcaatttga
    12541 atggacaaag acgtgaaatc actatcaaag ataattcgat catcagtacg gtccaaaccc
    12601 gattgaaagc tgaaccgaca aaccgagaac aaattggagc aacgatgtct ggttctgttt
    12661 tggatgtatt agtgaaaaaa ggcgataacg ttaaaaaagg tgatacgttg atgattactg
    12721 aagcgatgaa aatggaaacg gccatcgaag cacgttttga cggagaagtc gcacatgtat
    12781 atgtgtcttc aggagacact atttcgtctg gtgacctttt gattgaagtg actgagaaat
    12841 aataagcgaa aggtggggaa acatgaaaag actgggtttg ttttggctga ttctgctatt
    12901 agttattgtg gcattctaca tggaaccagt cttgtttccg ccaaaacttg aagtgaagga
    12961 aaactctgat caacaaatat caaaccaaat gacaacgtgg aaataccagg agcttaaagc
    13021 aactggtttt tctacttata tcggaaaacc gatcgatcag ctggaaaagg attttggcca
    13081 agcctatgat cgattaacta gcggatttgg ttttgaaaca agatattatg aaaataaagc
    13141 tagtcatctc acatttgaag cgaacatcca agacagcaga gtcacaactg ttaaagtact
    13201 gcaaacaggg cctgctgata tcgcgccgtt ttatttaggg atgacgatgc aagatcttac
    13261 gaaaatcact actatctata ctaatttcac ttttgagtac catgatacag aagttggaat
    13321 cgaactaatg gaagaggata tgaattatcg tccgctaatt gcttttgata acgacacttt
    13381 tgctatttta ttttttgatc aaacaacaaa cggcctattt tcagttgtgt atttagataa
    13441 agatagtctt ttgaaactat tgccatatca agtgtttggt gaaggccttc cacattatca
    13501 agcagataaa tcggctgatt gggaatcaat caatcgcgtt aaagaacgaa aaagtacgac
    13561 tttattaaat atgcttagaa gagaagacga acttcccata tacaatcaaa cacttgcctt
    13621 tcaaaaccac agtcagctgc tactacatga ttttttgaca aggcctgagg aaatattgtc
    13681 agaggaacgt atggctgatt ggcaaaaatc gctaggcagt gctcaaacga cagtgaaatt
    13741 ctcactgacg aatgatgaat tggagaagtt aatcagtaaa caaaaattag aaaatactag
    13801 tgggctattt attcatccgg cgattgacgc tactttttcg tttttatatt ggtacagcga
    13861 tccttacaac catgaccgtt tcatggaccc gggaacggat tctgtaggca ttgctttttc
    13921 taaagaaaat atgttagtct tattgcaaga agaaaacgga gaaagtagtg atagcagtga
    13981 tcgttaacga agagctgttt caactggagg atcaatgcct ccaagtcgct gaaatgataa
    14041 aaaagagtaa aacgatggct tcttatcttg aacataaaca gtcaatggaa gaatctgcaa
    14101 aagtttgcca gttgaaagaa gcgttcttgt ctaagaaaga agcatttgaa aatgtagcgg
    14161 catatggtga gtatgcacct gattatcgtg aaaaaaaacg agctgttcga aaagcaaaaa
    14221 gacagcttga tatggatgag catgttgcag ctttccgata tagtgaaaca cagctgcaaa
    14281 gtctgttgga tcaaattgtc caagtacttg ccgaagaagt ttctaatgaa atcaaagtag
    14341 atgcaggtag cccgtttttt gaaacgaaaa aacactttgg atgcggaggt aattgtcatg
    14401 caattagaca atagtgaatt tgaaataaca aaacgacgcg ggctgatcgt atgggtctac
    14461 agcttaaaac aattaaagaa tctgaaaaag ttcggcttgt tgcattatgt ctcaagaaga
    14521 atgaaatatg tcgtgcttta cttaaatgaa gagacattgg aagaaacaga agaacgaatc
    14581 cgtaaattac attttgttcg aaacgtagaa cgttcttacc gaccggatat cgaaatgaat
    14641 tttgccgaaa aaataggaaa caaaacagta ttaaaagacg aaggtttcga aattgaagag
    14701 ctaaatacag aaatccgatt agctgaacat ctttaaaaat ctcttatgag cagtttttaa
    14761 ataaaaaata acttggataa cgatcgattt ttgatcattg tccaagtatt tttttggtat
    14821 actagtacca gtaccatttc taaggagtga gtagatgcgg ataatatcag gaaattatgg
    14881 cgggcgccgt ttaaaaagtt tagcaggggc gaacaccaga ccaactacag ataaagtaaa
    14941 agaatccata ttcaatatga ttggaccata ttttgatgga gagacagtat tggatctgtt
    15001 tgcaggaagc ggtggtttag ctatcgaagc agtatctaga ggctgcagtc atgcggtatg
    15061 cgtagacaaa aattaccaag cgctaaaaat catcaaagaa aacatcgaaa ttacgaaaga
    15121 accagagaaa tttaccacaa taaaacttga tgcggacaaa gcaatcgaac ggctagcagc
    15181 ggagaaacaa caattcgact acttgtttct tgatccgcca tatgcaaaac aaaaaataat
    15241 cgatcaaatc cataaaatgg aagaactggg acttttttct aaaaccgcag ttatcgtatg
    15301 cgaaacggat aaaacagtcg aattgccaga aactgtggct ggattccatc aaataagaaa
    15361 acagatttat ggcataacgg ccgtaactat ctatagaaaa gagggaaatc aatgaaacgt
    15421 gcattattcc caggaagttt tgatcctttt acaaaaggtc acttagatac ggtggaacgt
    15481 gcggctaaat tattcgatga ggtagtaatt ggtgttttta tcaatacttc aaagaaaagt
    15541 ttgtttcccc cagaagaaag aatgacattg atcacaaaag ctgtttctca tcttcctaat
    15601 gtaaaagtca tgcatcaaga aaatcaattg acggtagaaa cagcaaaaga gattggtgca
    15661 gatgcgttga tcagaggcat acgaagtatc aaagactttg aatatgaacg agagattgct
    15721 cagatgaatc atcatctata tgatgaattg gaaactgtct tcttattagc aaaaccagaa
    15781 tatagtcacg tgagttccag tattttaaaa gaagtactgc actttggcgg agatgtgagc
    15841 agctacttac cgccagtgat caacgaagct ttagctggaa agcgtgaatc aaatgaaagc
    15901 taaacaatgg ttgaaaacag tactgattat catatttgga atgttgatca ttggatttgt
    15961 agtagttccc ttgccgtatt atgtagaatc accgggagcc acgatcgatc tgaaagaact
    16021 aattacggtc aatgataagg aagataaagg ttctgggtct ttctctctta cctctgttgg
    16081 tatcaggcaa gctacaggtt tcttagcttt aaaagctaaa atgacccctt ttgaagaatt
    16141 gatcagtgaa gaagaattga ctggtggggc gacaaatgaa gaatataatc aaatgcaaca
    16201 gtattatatg gattcttccc aaaatgcagc aatcgaacaa gcgttaaaac ttgcagacaa
    16261 gccttataaa atggaattca aaggcgtata tgtcatgagt gtagagaaaa catcgaattt
    16321 ctttggcaag ctttcagttg gagatacagt aacaaaagta gacgggaaaa gctttcaaag
    16381 tacagaagca tttatggatt atgtaaaaag ccaaaaagtt ggacaaacaa tcgaaatcag
    16441 ttatttgcat aatggtgaag aaaaaacagc ttctggtgat ctgatcgagt taccaacaga
    16501 taaaaaggca ggaataggta tcacgcttac tgaccatacc gagatcgaat cagatacaga
    16561 tgttcgtatt aattctggct ctatcggtgg gccatcagcg ggattgatgt tcactttaga
    16621 aatctatgaa caagtcactg gtaaaaactt gcgacatggt aagcaaattg caggaactgg
    16681 aacgatcaac agtgaaggag aagttggccg aatcggagga atcgataaaa aagtggcaag
    16741 tgcggataaa gctggcgtag agattttctt tgcaccagat gatacgattt cagacgatgt
    16801 taagaaagaa tatcctgaaa taaaaaccaa ctacgaagaa gcaaaagctg ccgctaaaaa
    16861 attgaattcc tcaatgaaaa tcgtaccagt aaaaacagta caagaagcgc ttgactatct
    16921 agagaaaatg aaatgaaact aaataattaa agaggggttg tgacacga
//
LOCUS       DAYYCY010000046        16958 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000046 DAYYCY010000000
VERSION     DAYYCY010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16958
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.046"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..356
                     /locus_tag="ACHAZB_001217"
     CDS             <1..356
                     /locus_tag="ACHAZB_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8613904.1"
                     /translation="RQKVFTKKDIENQINGFIQKLENANSYLINKELNKAYDQWKEVY
                     DELKLIQNETKLVRTEKKNENSFFFDGYAILMLETVAKQNIKAPKKELSDNIDNALAE
                     LRYYVMQIKDVRITR"
     gene            393..560
                     /locus_tag="ACHAZB_001218"
     CDS             393..560
                     /locus_tag="ACHAZB_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8613905.1"
                     /translation="MYRPQYLEQKYEVITVQNGNGEIVRKYRRPIKSDTYKRKESNEV
                     IPLYGKRIAKH"
     gene            586..930
                     /locus_tag="ACHAZB_001219"
     CDS             586..930
                     /locus_tag="ACHAZB_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFM8613906.1"
                     /translation="MNEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            935..1216
                     /locus_tag="ACHAZB_001220"
     CDS             935..1216
                     /locus_tag="ACHAZB_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8613907.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            1319..1633
                     /locus_tag="ACHAZB_001221"
     CDS             1319..1633
                     /locus_tag="ACHAZB_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HFM8613908.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            1611..3305
                     /locus_tag="ACHAZB_001222"
     CDS             1611..3305
                     /locus_tag="ACHAZB_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFM8613909.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNANLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            3325..4503
                     /locus_tag="ACHAZB_001223"
     CDS             3325..4503
                     /locus_tag="ACHAZB_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFM8613910.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            4466..5152
                     /locus_tag="ACHAZB_001224"
     CDS             4466..5152
                     /locus_tag="ACHAZB_001224"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFM8613911.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            5152..6312
                     /locus_tag="ACHAZB_001225"
     CDS             5152..6312
                     /locus_tag="ACHAZB_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFM8613912.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGRLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            6322..7197
                     /locus_tag="ACHAZB_001226"
     CDS             6322..7197
                     /locus_tag="ACHAZB_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8613913.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWARLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            7194..7505
                     /locus_tag="ACHAZB_001227"
     CDS             7194..7505
                     /locus_tag="ACHAZB_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8613914.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            7495..7848
                     /locus_tag="ACHAZB_001228"
     CDS             7495..7848
                     /locus_tag="ACHAZB_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFM8613915.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            7838..8239
                     /locus_tag="ACHAZB_001229"
     CDS             7838..8239
                     /locus_tag="ACHAZB_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8613916.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            8232..8636
                     /locus_tag="ACHAZB_001230"
     CDS             8232..8636
                     /locus_tag="ACHAZB_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8613917.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            8648..9256
                     /locus_tag="ACHAZB_001231"
     CDS             8648..9256
                     /locus_tag="ACHAZB_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFM8613918.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            9276..9638
                     /locus_tag="ACHAZB_001232"
     CDS             9276..9638
                     /locus_tag="ACHAZB_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8613919.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            9641..9823
                     /locus_tag="ACHAZB_001233"
     CDS             9641..9823
                     /locus_tag="ACHAZB_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8613920.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            9840..13259
                     /locus_tag="ACHAZB_001234"
     CDS             9840..13259
                     /locus_tag="ACHAZB_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFM8613921.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNTLAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     VTAGQSASESEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLTKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDYAVAIGGLTAAFTLLMPIIVGFMALGGPTTL
                     IIGAVITAIAGVIAIIKNWGAITDWFKGIWSKFTDWLGGTWESIKEGASSVWDGVKET
                     WSGFVDWVQEIWQGVSDWFGELWSGLVEGASNIWQGVQETWQAFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTSISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWSSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISSVFDNLNISIPHIPLPHFKLSGEF
                     NPLKGKIPTLGVDWYAKGSVFNSPNIIGVGESGPEAVLPLKRSVLQEIGDRILSSTSV
                     SSRTQAVQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKVQNDNASAWR"
     gene            13310..14047
                     /locus_tag="ACHAZB_001235"
     CDS             13310..14047
                     /locus_tag="ACHAZB_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFM8613922.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            14057..16348
                     /locus_tag="ACHAZB_001236"
     CDS             14057..16348
                     /locus_tag="ACHAZB_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFM8613923.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYPISGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKALAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLHHEKRLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLINDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGTDYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            16372..>16958
                     /locus_tag="ACHAZB_001237"
     CDS             16372..>16958
                     /locus_tag="ACHAZB_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HFM8613924.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDSVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIQKYNETFDAFMAEIEGRVDSLNKQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSI"
BASE COUNT         6060 a         2469 c         3560 g         4869 t
ORIGIN      
        1 taagacaaaa agttttcaca aaaaaagata ttgaaaatca aattaatgga tttatacaga
       61 aattagaaaa tgcaaattct tatttgataa ataaggaact aaataaagct tatgaccagt
      121 ggaaagaagt atatgatgag ttgaaattga ttcaaaatga aacaaaatta gtacgaacgg
      181 agaaaaagaa tgaaaatagt ttcttttttg atggatatgc tatattgatg ttggaaactg
      241 tagcaaaaca aaatataaaa gctcccaaaa aagaactatc tgacaatatt gataacgcat
      301 tagccgagtt aaggtattac gttatgcaaa taaaagatgt taggatcact cgttgagtgg
      361 tctttttatt ttgcacaaag gaggtaacaa caatgtatag accacaatac ttagaacaga
      421 agtatgaagt aatcactgtg caaaatggta acggagagat agtacgaaag tatagaagac
      481 caataaagag cgatacatat aaacgaaagg aaagcaatga agttattcca ttgtatggca
      541 aaagaatagc taagcattaa ataagattgt gaaaggagac gggacatgaa cgaggaattc
      601 tatagatggc tattacagtt gataagagaa gatcgtttgg ttaagttcta tcagtctcct
      661 aaatggcgca ggcttagaga gaaagcgatg aaacgagatc actatgaatg ccaagagtgt
      721 agaagactag gtaagtatca tagagtagag aacgttcatc atataaagga agtcaaggat
      781 agacctgact tagctttaga tttagataat cttatttgtt tatgtgttga acatcataat
      841 gaagttcatg gcagatatct tacagcattg gataaacaag agaagaagat agaaagcttt
      901 gctaacttcg atgcaagtga aaggtggtaa gtgcatgatc atcaatgata atggcagaga
      961 gtatgataca gaaaagattg aagagtattc atcttatact cagggattaa ttaaacgttt
     1021 gatatacgtt cgctatgtag gtattaggga tctgttatca gataactgtt gtagtaaata
     1081 caaagtgaat caagtaagag aagcgttgaa taaagataat aacgtcgaaa gaataaaaaa
     1141 tgtttttgga tatagtattg aagagattaa ttattacatt gacttcgctg aagctttcat
     1201 tccgatggtg agataacccc cccttaaaat aaatcgcaaa ttttttgggg gtgatgaaac
     1261 ggagggggct gtcaggaaaa gagatttttt cgaactttat catgaaagga gggctaaaat
     1321 gtttaaaaac gaattgtctc aaaatcgcta cagagaaaaa ttacgccgct ctttaataag
     1381 ccaattggaa agtcagaaaa caaatattga gccattctta gataatgttg atcgttatat
     1441 cagtttatgg gaaacggcga tatcactgga agaagatata tccgagaacg gcattagact
     1501 ggagaatggt aaaaagaatg aatcagtagc gttgcttgtt tctgtcaaca aacaaatggg
     1561 attgatgttg gataaacttg ccattactcc tgaattggta ggtgaagcaa atgaatcaat
     1621 tcctgagtta taagcatatt gaaaattggt tcaaagctat agaagaaggc actatcaagg
     1681 tatgcaaaga gcaattattg ctaaaaaatt atctagaaga aagagtcttt actagagaag
     1741 atatttactt cgataagcag atggtagagg attcaatcaa tataccagca caatactttc
     1801 cattcgaatt aattccgtgg gaaaaatttc tacaatgttt tatttatggt gttcgatgga
     1861 aaaaagataa aacactagtg ttcaatagat atctttcatt aatgggacgt ggtaatggta
     1921 aaactggttt tgcttcttgg aacaacttct ttctactaac cgctaaacac ggtattaaaa
     1981 attatgatat tgatatctat gccaataatg aaagccaagc aaagactagt tttgatgatg
     2041 tatttaaagt aattaaagat catcctgatt tagataaaaa agtatttaaa gctacgaagg
     2101 aagttattca aaatatcgct acaaacagca aacttcgtta taacacggca aatgctagaa
     2161 caaaagatgg gaagcgacca ggtgcaaacc gctttgatga aattcacgaa aatgaagatt
     2221 attcaatgat aaatgtggct acttctggtg gtggtaaaat tcgagattat agagaatttt
     2281 atgatacaac taatggtcat gttcgtggtg gtccgcttga tgacattata gaagaatcaa
     2341 aaatgattct ttctggagaa cttggaattg acaaggatgg agcagaattt tctagtttgt
     2401 ttccatttat ttgtcgcttg gataacgata atgaagttga tgatcccgac atgtgggaaa
     2461 aagcttgtcc aactattaat tacaatgcaa atttaaaacg gaaaatgttt caagaatact
     2521 ctcaaatgca acgtaatgct ggtttaagac ttacgttcat gaccaaacga atgaacagac
     2581 ctatggaaga tacacgattt gctgttgctt catatgatga tgttctgcat acgaaagaaa
     2641 aagaatttcc tgaaaaaatg gatgaagtga taggaacagt cgattttgct gatagacgag
     2701 attttgccag cgttgggttg ctaggaaaat acgataaaga tgtttatttt acacaacata
     2761 cttttatcca cgaatcagcc cttcgattac aaaacatcaa acgagaggtt atagatattt
     2821 ctatagatca aggaaaatca cagatcgttc atggaaaaaa tatagaagct gattatattg
     2881 taggttggtt tcttgaaatg agtaataaat attatattaa aaaaatcgct atggatatgt
     2941 accgtgcaaa aatattgaag cccgctttag aagaagcagg ttttactgtg gaaattgttc
     3001 gaagcggatc tgttacacat ggtatgttaa aagatctggt tgatgacctt tttattaatc
     3061 aacgtttatt ttttggtgac gatgcgatta tgcgttggta ttgcatgaat gtatatgaag
     3121 agcatatttc taatggaaat atacgctatg aaaaaataga acctgaaact agaaaaacgg
     3181 atggcttttt ttcattcctt catggtttga attttttaga tgatatttat gattctgctc
     3241 ctgtaacagt cacaaatagc tcagtagaaa atacaggaac tggatttact cctctagtat
     3301 tctaacttga aaggaggtga gaaagtgggg atttttcaaa aggcggtagg atacttcaca
     3361 aaaaaagcaa cggttccttt agaagaatac ttttgtaaat tgcaagttga ttttgtgtat
     3421 cgaaaatttg cgattgaaac ttgtattgat ttgattgcaa atgcgatgag taaagcggaa
     3481 ttcaagtcat atgaagatgg aaaaaataaa aagaatgatc tttactatag gctgaatgta
     3541 gctcctaata agaaaaataa tgcaacagaa tttagaaaaa aactgatcag gagattaata
     3601 ttctacaatg aagtattgat cgtttctccg tctaataatt ctagcgaaat atttattgcg
     3661 gatagttggg atgtcacaga atatgcattg aaagatgatg tgttttctca agtgcaaatt
     3721 aacaacatag tccttgatag agaatttcta gaaagtgatg ttatctatat aaaatacgca
     3781 gatcaacaaa ttaggcaact agtcgatgcg tattatcaag cgtatgggaa actcatttct
     3841 agtgccatga atgtttacaa gcgctctaac gctcgtagat acgtactgaa agggaattta
     3901 ttccgaccgc aagacaatac aacacaagat caaatcaata aaatgatgac atcacaattt
     3961 aaggctttta tggaagctga taatgcaggt gcggtatttc aattacaaaa tgagtacaca
     4021 ttagaagatt tcagcggaaa cttccaaagc aattcaagag atataaaaaa cttaatagac
     4081 gacatctttg agatgacagc agcagcgttt cacgttccga aaaacctact aaagggagac
     4141 atgagtgggt tatcggatca agtggacgct tttttaatgt tcgaaatcat accgattgct
     4201 gaacttattc aggatgcgtt taacgctagt ctctatgaag tagaagaata cttgtcaggg
     4261 aattttgtac gtgtggatac aactatgatc aagattacta gcttcaaaga tttggttgac
     4321 gctattgatg taggcattag aaatggagta tttacaatca acgaaggaag agaacgcgtt
     4381 ggaaatgatc gctctgataa ggcgatggca gatgaaatat ttataactaa aaacaatcaa
     4441 caagtatcga aaggaggtga ggcgaatgac gacaatgaaa acatttctag cagtaaagaa
     4501 tgaaggcgca gtaccgcaaa tttttattca gggatttatt ggttctagtt ggttctttga
     4561 agggaatact gacaagggaa tcaaaaatat tttggatagt ctaggtgatc aagaagaaat
     4621 tgaagtagta attaattcaa acggtggaga cgtatttcaa gggattgcta ttgggaactt
     4681 acttaagtca aataaagcaa aagttaacgt tgtgattaac ggcttagccg ctagtgctgc
     4741 ttcaattatc gcaatggctg gcgatactat aaaaatctac aacaatgcac aattgatgat
     4801 tcaccgcgct tccacatacg gagaaggaaa tgtcgatgac ttccgtacga ttgctgacca
     4861 actggaatca attgataaat cggtaaaggc ttcatataaa acacgattca atggcacaga
     4921 tgaagcattg caagaacttc ttgaaaaaga atcgtttatg gatgcagaaa cagctttgag
     4981 ttatggattg gtcgatgaaa ttatcgatgc agaaaatagc tcaggtactg aagctaaaaa
     5041 agaacaaagc gttgaagaaa ttttgaatga cgttaaagaa aaaagagcag aaaaaattgc
     5101 tgcatttaca gcagcattaa ataaaacatt tggacaagga gatgtaaaat aatgacagtt
     5161 aaaaatttaa aaggtgtaac agctgcaagc gaccaattga tgaaagcttt taaagatggt
     5221 aacgaagaat cttttagcgc agctatggta agcttatcta aggaaattca ggataaaatt
     5281 ttagaagaag caacagcaaa aaatcaagat caattagtat taatgaaccg tggtcagcgt
     5341 gtattaacta cacaagaaac aaaattctat aacgaagtag tgaaaaacga aggttttgca
     5401 ggggtcgaag aattagtgcc agctactgta tttgaacgtg tatttgaaga tttagaacaa
     5461 tctcatccac tattgcaaaa aattactttt gttaacacaa ctggtgtaac agaatggatt
     5521 gtgtcacgtg gagtcaatcc agcatggtgg ggtaaacttt gcgaagctgt taaaaaagtt
     5581 ttagataatg gctttgatgt aattaacatg aagcagttca agctatcagg ttatattcct
     5641 gtatgtaagg caatgcttga tttaggtcca gtatggttag atcgttatgt ccgtactgtt
     5701 ttagtagaat cattgagaat tgcattagaa caagcaattg ttgatggtac tggtaaagat
     5761 atgccagtcg gaatgatgcg tgacatgagc aaacaaacta gcggagaata tgctgaaaaa
     5821 aaagcagaac ctattacagc tttagatgct gtaactatgg gccgtttgat ggcgcgacta
     5881 tcaaaattca atatcgaagg tgtgaatgat ccgatttatc gtaatgtgaa tccttctgat
     5941 gtggtcctaa ttgtgaatcc aactgattac tggtctaaag tattcccagc taagactgta
     6001 ctaactgcta atggagaata cgtacaagta ttgccagtac cagtttcaga tttgcagtca
     6061 acggctgtgc cagaaggaaa agcagttatt ggggtagcct cagattactt catgggtgta
     6121 ggatctacac taaaaattga agcttcagat gaataccatt ttgttgaaga cgaacgcatt
     6181 tatctagcta aacaatatgc aaacggtcaa cctaaacgta acgatagttt cattgtatta
     6241 gatattagcg ctttgggaac tactactaca actacaaaac caacaaccac aacaactaca
     6301 acacaagcgt aggtgatcag aatgaagtat attctttgtc agccggcaat caatcggttt
     6361 aaatgggagc ttgaagtttg tttaactaat ctgaagaaac taggaatcaa agatatcgta
     6421 ttgcttttca gcagacacga tgatcagatt cctatttttt ttgagaagga atatggtgtt
     6481 gaagttcatg tgtacgatga tctgcgggac gacaaagagt atattccttc gattaaacca
     6541 tatttatggt ggaaatattt agaagaagat cattcgcgtg aggacgaccg atatttctat
     6601 atcgattcgg atgtcatttt caataaaaga attaatttgc gcaaattgcc ttctaaagat
     6661 gatgtttggt attgtagcga ctgctgtagt tatctaagtc ttgattatat tagaagctgt
     6721 gaaaacggag aaaatattct aaaagatatg gcaaacattg taaatgttac agtagaatct
     6781 ttggaaacta taaacactaa ttcaggaggc gcacagtggg ttattaaccg tcctaaagcg
     6841 aattattgga aaaaggttta tctggattct aatcggctat atcgctacct tagagggcaa
     6901 aaaacaaata tacaaatttg gacagccgag atgtgggcac ggctttggaa catgatgtat
     6961 ttcaatattg gtcctaaagt tcacgaggaa ttagactttt gttttgctac tgatccaata
     7021 gaaaaagtta aagaagtaaa aatcttacac aatgctggag taacaacaaa cgatgaagat
     7081 ttatttttca aagggagata cgtgacttct acgccttttg atgaagattt atcatttgta
     7141 aacaagaaaa aatgctctta cgcatatgtt aaagcaatta aggcggtggt tagatgacgc
     7201 ctgaacaagt gactgaagaa ttgctaatag ctgtgaagga taatatttac gttacctgga
     7261 acgaagaaga tgagtcaatt aaaaagatga tagctaaaaa tgctgtttat cttcaaagta
     7321 aagtgagtac aacactttct ttttctcctg aaagcttaga atacggattg ctaatcgaaa
     7381 gatgtagata cgactggaat cgtgctttag atgagtttga acaaaatttc gctagtgagt
     7441 tattaggttt cattcaacat tatgcgctac aagaatatat tgcaggtgat gtgaatggcg
     7501 aataatcgta gactcgaaga aacattcaac gatggttggt taaagatttt gacgcaaact
     7561 accaaaagaa atgaactagg aaaaaagatt ggtgtagaag atacagaaat cacttcttta
     7621 aaatttagaa atctttccat gagagatagt gatataacag ctatggatgc gatgggatcg
     7681 aaattaacta agaaagtaaa gactccattt catccaatcg ccaagaaatt taataaagat
     7741 caatatttta tcgtaatcga tagtatgcgt tacaacgtta tctatgccga ttacgataat
     7801 ttttatatct atttttatct tgaaagtgtg ggtgaatatg gtgattgata attctaaaga
     7861 aaaagaacgt ttaaataagc aaatttctgc tatcaaaact tccttagaag agcattttgg
     7921 cctcaaactc tttcaagact ccgtaggcga ggatgagcta cctgatgatt ttaattactt
     7981 cattctcgaa acaggagaaa tagaaatgat cactgagcca aaatatagcg tgggtcaaaa
     8041 tctatatcta actttctatt cagaaaatag agaagattta acaggagact cactagatat
     8101 tatttcattg attcaaaatc gttcgattcg ttttcagaga atggatccca atcatttaaa
     8161 actagagaac caagatcgct atatcgatca attggtattt acgtttagac gattattgaa
     8221 gagtgattgt catggctaaa aatagttggg agctaaaaat aaatggacat gatgaacttc
     8281 ttgtgcggat ggaacgctat tcaagcgaga gcgaacgact gattaacgaa gcattgaaat
     8341 caaaaggttc ggctattgca gtggatagga ttacagaaaa aattcctgtt tctgaagcag
     8401 atttaagaag agggcaccaa cacgcaaaaa atagtcgtcc acttaagact caatacatta
     8461 atttgggttt catcattaga cctacaagaa aatttgagta tttaaaatat cctgatttgg
     8521 ggataggtac ttctaaaaga aatcagccgg acgaatttat gagaagagga ttaggtcttg
     8581 cacttgatcc aattacagaa cttctgattc gtcaattcga taaattaaat aaataggggg
     8641 aacaacaatg gctaaaacaa aaactgtagt aacaacgttc gataacgtaa gtatcaaacg
     8701 aattgctttt aattttaaga acgcaggaaa tgcaatcgca acgaattgta atggacaatt
     8761 agatggcgaa acagaaatgc aaacggtggt taaaaaatgt ggagcgacag aagtaaaatc
     8821 aaaatctaaa ccaatcaata tgacggtaac aattactgca catgtaccga tggaagttta
     8881 tcgacgtttc aatgggttga aacaagatgg acgtattaaa ccaggcattt actcttacgg
     8941 tcctgattcc gtaggcgaag atttctcact tgctgcagag atcgtggatg acttcgaaga
     9001 aaatagaaag ttagttggta tgttagcatg cacttcgaat acaggattaa cattctctat
     9061 tgaaaatggt gcggatgaag tagctgcgtt agaactagaa acaaaagtta tgcaagatga
     9121 atttggtaaa ttttatcatg aagcaattgt tgcagaactt gaagaagact taacagatca
     9181 atggatgacg aatctatctg ctgatgtgat taaaaaaact tcagttgtga caactacggc
     9241 cactcaatca cagtaaaaaa aacggaggta gcgaaatgaa cgaagattac tcaaaaattg
     9301 aactaaacga tggaacaatt ttgaatttag aacctaaact gaatatcaag aaattattga
     9361 tgatcaatag agattttaac acagacgagt ttgcaaaaat gactgtggga aaaggatcca
     9421 tggatatttc tgttattcaa ggtgcaaagg ctgtgtatat tgcttaccgc caagcgaaca
     9481 tgactgatta tatttcattc gatgaattta tcgataaatg ggattttgat atggctactg
     9541 ccagctatat ttatcaattg atgatgttca aacaagcacg cgatgcttat caaaaagaat
     9601 ttgaaaaagc aaataaggaa aaaaagcttc aaaagtaaaa atgccaaagc tcttagttga
     9661 aacgtgggtc gatgtctatt cgatgttgac cgacgttttt tctatgcctt cagatttggt
     9721 tttaagcgat atctgtttag atgacatttt gcaaatggct tacaacaaga gtgcttatga
     9781 aggatggaaa aactatgcaa taaaccaatc tcagaaaaac taaagaaagg aggtaaaaaa
     9841 tggctaaaaa gagaacagaa gcagaagtaa ctttcatagc taacgatgac ggattgaaat
     9901 ctacgttaaa agaaatcagt gctgaattaa ctaaaaatag agcagaatta aaactagaac
     9961 aagctcaatt acaacagact ggttctgaat cagacaagtt aggaagtaaa ttatcttctt
    10021 tagaaaagca gtatgaatta caaagccaaa aagttgaagt aactagccaa cgtttagcca
    10081 atgccaaaaa atattatgga gaaaattcca ccgaagttca gaaacttgag agagaactga
    10141 ttaaccaaca aacagcgcaa caacgtttgt caaacgaaat tgataaaacg agtaatacac
    10201 tagctcaagc aaaaggcgaa atacagacgt acgagtctac aatgcaacag ttggatagtg
    10261 aacaaaaaaa tgttcaagct agtgcttctc tgattgaatc cgaatacaaa aaatggcaag
    10321 taactgctgg tcaatcagct tctgaatccg agaaattagc gaaagcccaa gaatatgttt
    10381 ctcaacaatc tgaaaatgca gagaaaacga tagatatcct gagacgacag ttagaagcta
    10441 cacagtctga gtttggcgct acatccacag aagcaatgca gatggaggcg aagcttaatg
    10501 atgctgaacg tgaatttgaa gagttaggac aagctgctaa aaatgtagat acaactaact
    10561 tggacgatat cggaagcaaa atagatatga ataatttaat ggaagcttct gacgttttaa
    10621 gcgacattgg cgataagctt acagaattag ggaaacaagc agtggactct gctaacagtg
    10681 taggtagttc ccagagtaaa atacaagcta attttggttt gactaaacaa gaggctgaag
    10741 aattaacgaa tgtagccaga gacatttatt ataaaggttt tggagaatcg ttagatcagt
    10801 ccacagatgc attgattttg gtaaagcgta atttaggcga tttaaataat caagatttac
    10861 aaaatattac ggaacaagct atggtcctag aaaacaccat gggcgctgat atggatgaaa
    10921 cgttacgtgg tgtaaatggc ttaatggtca acttcggatt gagcgctcaa gatgcaatgg
    10981 atttaatggt ttcgggtact caaaacggtt tagataaaac gcacgaatta ggcgacaata
    11041 tggcagaata tagccaatta tggagtcaaa tgggatattc agctgatgaa acgttcggaa
    11101 tgcttcaaaa tggtttagat gcgggtgctt ataaccttga taaagtcaat gacttagtta
    11161 aggaaatggg aatatcgtta acagatggtc gatttgagca aaacatggat atgtttagtg
    11221 aaagtactag aaaagctttt gaagagtgga aaaatggtgg aggaacacaa aaagacgtta
    11281 ttaattccat gattcaagat tttagcaata tggatggtca atacgaccaa ttaaataaag
    11341 cttctacaat ttggtctgca cttggcgaag ataacgcgat gaaagttgtc caatctttga
    11401 ctgatgttaa ccatacattt gatgatgtta gtggatctgc acaaaaaatg aatgaagatt
    11461 ctactactcc gttacaagaa ttaaacggaa aaatagctga attaaaggat tcattagctc
    11521 ctataggcaa cacaatcata gatgcactcg aaccagtaat tgattttcta ggaaagatgg
    11581 ctgatgcgtt taataatctt ccacaaccag tacaggatta tgccgtagcg attggcggat
    11641 tgactgctgc atttacttta ttaatgccaa taatagttgg cttcatggct ctaggtggtc
    11701 ctactacatt aataatagga gcagttatta ctgctattgc tggagttata gcaattataa
    11761 aaaactgggg cgcaattact gactggttta agggaatatg gagtaaattc actgattggt
    11821 tgggtggtac ttgggaaagc ataaaagaag gtgcctcatc agtttgggat ggagtcaaag
    11881 aaacctggtc tggatttgta gattgggttc aagaaatttg gcaaggagtt tctgattggt
    11941 ttggagagtt atggagcgga ttagttgaag gagcttccaa catctggcaa ggagtccaag
    12001 agacttggca agcattcgtt gattgggttt caaatatttg gaacggagtc aaagaagtat
    12061 ggtcgattat ttgggcagac attgtaggaa ttgttcaaat accatggaca ttaataacgt
    12121 cattgattca agctggtatt aatattatcg tgggtatctt tgatgtagct ggacagttat
    12181 taggcgcagc ttggcaagct gtttggacac ctatttctga tttccttaaa aatacttggg
    12241 atactatgac acaatggata agcatcgctt ggaatggaat tgtaactaca ttccatacta
    12301 tatttgatcc agtagtggca tggtggaatg gtatatggac atctattagt actacggctt
    12361 caaatatttg gaactcaatt agtgcaacag cttctagtat ttggaatagt atcaagaata
    12421 caatcactag cttggtacaa gcagctgcta cagtaattca aaatatttgg tcaactgtat
    12481 ctagttggtt aggtggaatt tggaattcaa tcagctctac agcatcaaat atctggagta
    12541 gcgtgactag tagtataagc aatgctataa acgcagctaa aagtgccatt caaagtgttt
    12601 ggaatagtat atcttcgtgg atcagcggaa tttggaacgg tatcaaaaac actgctttga
    12661 atctttggaa tggaattaca agcactatta gctctaaagt aaacgatgga aaaaatgcaa
    12721 tttcaagcgg ttggtccaat ctaacaggta ttgtttccga catattcaat aatgttaaaa
    12781 gcacgatagc taatatatgg gaaggtatca aaaagactgt tagcgctccg attgattgga
    12841 ttagagataa aatcagtagt gtctttgata atttgaatat ttctatacca catattccgt
    12901 taccacattt taaattgagc ggagaattca atccattgaa ggggaaaatc ccaacattgg
    12961 gtgttgattg gtatgcgaaa ggtagtgtgt ttaattctcc aaatattatc ggtgtaggtg
    13021 aatcaggacc tgaagcagtt ttacctttga aaagatctgt gctgcaagaa attggtgatc
    13081 gtatcttgag tagcacctca gtttcatcta ggacacaagc ggttcaacct gtgaacaatt
    13141 acgaattcaa tttcacaatt gatggtaacg cagatgaagt cactatgaag caaacaactc
    13201 aacaaatcat tgatagcatt acaaaagttc aaaatgataa tgcttcggca tggcgttaaa
    13261 caggagagta tttctcctgt ttttttagta ttaaaaagga tgtgaaaaaa tgactgattg
    13321 tatacattct ataatcgatg gatttcctga ttatttgcat aaattggctt tagctgaaag
    13381 accaaccata ccttctccaa aaagacagag agttgaaact tctgttttag gaaggttagg
    13441 tggcttagtg caagattact cgtttgaaga catgtcgttt acattgcact ataactattt
    13501 agaggatgtg gaagaccatc aagcgttcaa gcaatcgttt tatatcatgc gtcattggtt
    13561 aaactatgca aagaaattag aattctctga cgatcccaac gtctattacg ttatccagac
    13621 tatcgatatt ggggatgcag aaaacgatat tgttgaatgg ggagagttcg atgtaaatat
    13681 tactgcgaaa ccattcgcaa gagttcaaga agatgtacct ataaccgtag ataaaccaca
    13741 gtcatttaac ttgctgaata atagtttaga agaaagtttt ccaaagatta tcatcactcc
    13801 ttcagctact tcatgccagt tcatcttaaa tgattatgtg tttagtttcg aaggcttagt
    13861 agcaggaact gatgtagtca ttgatagtga tttgatgctt tgctacgaag agcaatcgga
    13921 cggagatatt ttagatcggt ccaacaaaat gaagaccatg caatatccga cattgcaagt
    13981 ggatatcaat cattttaatt gcactggttt gagcaaaata caaatttatc gtaacgggtt
    14041 aaggtaggtg aaatagatga tcgataattt aataactatt tacgataaaa atgacgcgaa
    14101 taatttagct gaacatttat atgatacgca aggtttaggc gctttgtcag actggttaac
    14161 agctactgtt agcaataaac taaacggagc cgagatattt cagggtactt atccaataag
    14221 cggaactaat gcagatttga ttgtagaagg acgtattatt cagtgttatg tagatgaaaa
    14281 tcgagcaaaa cagcgtctac ggatttatta tgcaaaaact tctgtaatag gcaatacgat
    14341 agaagtaaaa gctgaaccta ttttcaatga tataagaaaa tcggtgttga ataaatatga
    14401 cagcggaaca gaaaaaatca ctgctactca ggcatggcaa aacgcaaaag ctttagcgaa
    14461 accagctatc ccttcgcagt tttctttctc gtcattagta gatacgcttg ctaatgtgaa
    14521 gatagaaaaa gcgaattttt tagaattctt tggtggaaaa gagggatcta ttctagatcg
    14581 atttcatggg gaatttctta aagataataa cacattacat catgaaaaaa ggctaggtac
    14641 ggatcataaa atcaaagcga tttatactaa aaatttaact ggtcttgact tagagataga
    14701 tgctcaaagc gttttagttg gagtttatcc attcattagc agttcttcag aaggagaaga
    14761 cgagatcact ctaccagaag aagtcatttt cacggattac gtggatgatt atcctgctgg
    14821 atatgtttct tttgttgatt ttaaagacaa agcgactgat gtagccacat taagggaagc
    14881 tgctaaagac tggttgaaaa caaacataga taaacaaaaa ccacaagtga gtggttcgat
    14941 tgaattagta ccattgaggc atcaaagagg ctatgaaaaa tttgttgatc tagaaaaagt
    15001 ttcgatgggt gacggagtag atgtgtatca tccacagtta aaagtgaata tgtcagcgag
    15061 aattgtggaa tatacgttta atgttctaac caattcatac gataaattag ttgtaggaaa
    15121 cgtcaaaaca aacttcttag aaaacacaga gaataatgta agcaatttga ttaatgatgc
    15181 cattgatcaa ttgaaaaatg gtggcgaaat cagtgattta atcaatgata ttgtagatca
    15241 tcaaactgat atgattactg gtcaaaatgg tggttatgtt ttattagatc ctaaagaagc
    15301 gcctagtcgt attttgatta tggacacacc agataagaat accgcaagga atgttttaca
    15361 aatcaacaat gctggtattg gtttttctaa aactggcatt aatggaacat atgaaaccgc
    15421 atggacatta gatggcggat tcaatgcctc gtttattacg gctggtgaaa tagtaggaat
    15481 tactattaga ggtactacat taattagtga tggcactgat tatagaacaa gtattgctaa
    15541 tggcaaaatg acttggtact caaaaaaagt taacaaagat attatggagc tagaagcacg
    15601 tgattatgta agtgctgatg ccggtattgt atcatacacc atgaaaactg gtggtggttt
    15661 catgattaga aatccacagg gtaacttggt ttttagtacg tgggataatg gtaataacag
    15721 accgtttcta tcttttggtg cgcccaattt caggtatagc aatgctagtt atgtaacttc
    15781 tggcgacggt agttctttaa gcattaatgg tagtgcgggt aactcatggg aatttaaggt
    15841 agctggtagg actatgaaat ttactagtga tggtatgcta acgttgccag gttgtttttt
    15901 tggttcatgg gaagatggga aacttgctag gtttgaacaa tcaacggtac aagtatataa
    15961 agattttact gttagaggta ctaaaaactc aaccgtacca acagaacatt atggacaacg
    16021 actattaaac gcttatgaaa ctccagaata ttatttcgct gattatgggg aagccgttac
    16081 aggtgatgat ggtaaagttc gtgttgatat tgaccccatg tttgctgaga cagtaaatct
    16141 aagtcgatat atgacacatg tgacacctac agaactagtt ttgtgtgctg ttactcatga
    16201 agatattgac catttcatca ttgaaactag taagccaaac gtattagtta gatggaattt
    16261 agtggcacac cgtctagggt atgaagatat tagattaaaa gaggatacag catatgatag
    16321 cacagtgctt gaccaaaaac gtttttaaaa cgaagacaag gaggtatata aatggctagc
    16381 agtttatata atttggctct agatttcagc aaagaattaa actacaccaa agctattatg
    16441 gctcgtcaag gtgataaagg gattacagtg actgttaaac catttttaaa tggcttgcag
    16501 atggatacga gtggcggaac atttacttta aaaggaacaa cgccatctaa ccgttacgta
    16561 gatagtgtgg caactagtgt aactagtgaa gaagtcacgt tttctcttga tggcacattt
    16621 atgagtgaag caggatatta taaacactgc tacgtagaat atagaaaaga caatcaaatt
    16681 ttaacaacgc aagatatcat ttttttctca ctaggagtgt ctgacatttc gcaaggccaa
    16741 gccgatgaat acgtttcgca attggaagag ttgattcaaa agtataacga aacttttgat
    16801 gcttttatgg ctgaaatcga aggtagagtg gacagcttaa ataaacagat tactgattta
    16861 actggtcaag ctaaaacact acaagacaag ttagacgctc tgaaagaaga aatttctaaa
    16921 ttaggtaact tgcaggtgat gtacagtaac agcatcga
//
LOCUS       DAYYCY010000047        16530 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000047 DAYYCY010000000
VERSION     DAYYCY010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16530
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.047"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(29..727)
                     /locus_tag="ACHAZB_001238"
     CDS             complement(29..727)
                     /locus_tag="ACHAZB_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HFM8613925.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(720..2072)
                     /locus_tag="ACHAZB_001239"
     CDS             complement(720..2072)
                     /locus_tag="ACHAZB_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HFM8613926.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2295..3695)
                     /locus_tag="ACHAZB_001240"
     CDS             complement(2295..3695)
                     /locus_tag="ACHAZB_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFM8613927.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3856..4593
                     /locus_tag="ACHAZB_001241"
     CDS             3856..4593
                     /locus_tag="ACHAZB_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFM8613928.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4718..5197
                     /locus_tag="ACHAZB_001242"
     CDS             4718..5197
                     /locus_tag="ACHAZB_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HFM8613929.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5194..5961
                     /locus_tag="ACHAZB_001243"
     CDS             5194..5961
                     /locus_tag="ACHAZB_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6681 family protein"
                     /protein_id="HFM8613930.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6191..6760
                     /gene="lepB"
                     /locus_tag="ACHAZB_001244"
     CDS             6191..6760
                     /gene="lepB"
                     /locus_tag="ACHAZB_001244"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFM8613931.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6864..7466)
                     /locus_tag="ACHAZB_001245"
     CDS             complement(6864..7466)
                     /locus_tag="ACHAZB_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFM8613932.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7572..7802
                     /locus_tag="ACHAZB_001246"
     CDS             7572..7802
                     /locus_tag="ACHAZB_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8613933.1"
                     /translation="MPISFVKDREEKGKCVREILLDLPEWFGLPESTEKYIEESSKLP
                     LWCEKRKEEYLGFITLSQTSEDTAEIYSIVWE"
     gene            7793..8041
                     /locus_tag="ACHAZB_001247"
     CDS             7793..8041
                     /locus_tag="ACHAZB_001247"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8613934.1"
                     /translation="MGIKKDYHHQGLGTKLFKEAEGYAAKHYKYLQVKTVDEGHYSIY
                     DQTICFYESLGFSRLEVFPNLWDEWNPCLVLVKKLEQK"
     gene            8147..8731
                     /locus_tag="ACHAZB_001248"
     CDS             8147..8731
                     /locus_tag="ACHAZB_001248"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFM8613935.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8734..9807
                     /gene="prfA"
                     /locus_tag="ACHAZB_001249"
     CDS             8734..9807
                     /gene="prfA"
                     /locus_tag="ACHAZB_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFM8613936.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9800..10639
                     /gene="prmC"
                     /locus_tag="ACHAZB_001250"
     CDS             9800..10639
                     /gene="prmC"
                     /locus_tag="ACHAZB_001250"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFM8613937.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10632..10811
                     /locus_tag="ACHAZB_001251"
     CDS             10632..10811
                     /locus_tag="ACHAZB_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8613938.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10833..11858
                     /locus_tag="ACHAZB_001252"
     CDS             10833..11858
                     /locus_tag="ACHAZB_001252"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFM8613939.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12033..13277
                     /gene="glyA"
                     /locus_tag="ACHAZB_001253"
     CDS             12033..13277
                     /gene="glyA"
                     /locus_tag="ACHAZB_001253"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFM8613940.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13430..14377
                     /locus_tag="ACHAZB_001254"
     CDS             13430..14377
                     /locus_tag="ACHAZB_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HFM8613941.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14511..15140
                     /gene="upp"
                     /locus_tag="ACHAZB_001255"
     CDS             14511..15140
                     /gene="upp"
                     /locus_tag="ACHAZB_001255"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFM8613942.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15279..15521)
                     /locus_tag="ACHAZB_001256"
     CDS             complement(15279..15521)
                     /locus_tag="ACHAZB_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8613943.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15642..15714
                     /locus_tag="ACHAZB_001257"
     tRNA            15642..15714
                     /locus_tag="ACHAZB_001257"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15675..15677,aa:Thr,seq:cgt)
     gene            complement(15833..16471)
                     /locus_tag="ACHAZB_001258"
     CDS             complement(15833..16471)
                     /locus_tag="ACHAZB_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HFM8613944.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
BASE COUNT         5637 a         2949 c         3324 g         4620 t
ORIGIN      
        1 ttttgtcaca ttcccctttt tcgaataact aggctgtttg tccttcaaga tagggcagat
       61 ccaattcttt accgtaacgt tgttctaaag ctaggcggat caaacgagct gccaggttct
      121 catttcttgc aaggataggt ccatggaaat aagaaccgaa gacatttcgg taaatgacac
      181 cttcagattg gtcttcacca ttatttcctt gaccttgtac cactttcccc aaaggtcgtt
      241 caccttcacc taagaaagtc cgaccattgt ggttttcaaa tccataataa gtttcattga
      301 attcttcgtt atggataacg atatcaccga taaaccggtt attttcctga cttaacgtgt
      361 agtgatccag tgcactgatt ccttggatct tttctccatt agctcccatg tagtagtgac
      421 ctagcaattg gtagccacca caaatggcta acatgacgcc atcattttcg atatagttga
      481 tcaaagaatc tttctttttc tggatgtctt ttgaaatgat taattgctca aagtcttgac
      541 caccgccaaa gaaaaccaag tcatatttat cagcatcaaa aggttcatgt atactaacga
      601 tctccgtatg gcaagaaacc cccatttttt ctgctatata tttcaacatc aaaaggtttc
      661 cgttatcacc atatgtgttc atcaaatttc cataaaggtg ggcgatattg agttcataat
      721 tagccacggt tcattcctcc tttgataaat ccttgagcag ctagttcttt acgaagctgc
      781 aatacagcag tgtaagtagc taaaatatag acatgctcag ttggcaattc tttgattttt
      841 tcgattactt ctggtaattc ttttgtttct gtcaatttat tttctggaat cccggcaact
      901 tttagacgta aagtcatatc tgtatgacgg tctcctccag aaataacagc tgggatatcc
      961 atatcggcaa agttttcaaa attaccatcc caaatccagc tgacatcgat tccgtctgcg
     1021 taattggcat ttaataaagc cactaaagaa aatggataag gagctagacc aatcatatcg
     1081 attacttgat tgatacctac aggatttttg acaagtacta atgtacattt tttcccattg
     1141 atattgatga cttcttgtcg gccgaatact ttctcgtcat atcctaaacc ttgtttgatt
     1201 ttatcaggat cgacattgaa gtactctgcc actgcagtag ctgctaaagc attgtagaca
     1261 ttatacatac cgccaacttc gattccgtat tcatgaccat cgatcacgaa atctgctgac
     1321 acattgtcca tccgtaccat atcagttaat cgataatcca attcaggacg tttgaagtca
     1381 caatttggac agtaatattt ccctaagttt gcataagtga tcattttata atgcaagatg
     1441 tgatgacaat ttggacataa gacaccgtct gtattgtaat gagccatttg ttcacgatct
     1501 tcttcatggt caaagccata atattttcta ggattgactg tttcgataga attgaagatt
     1561 ggtgaatcac cgttactgat aatcgttgca tttggtgact ttgcggcacc gtctacaatc
     1621 attttatagg tcgtatagat ttctccatac cgatccattt gatcacggaa aatatttgta
     1681 aataagaaca gttctggttt catatattct gtgactttgc tcaaacttgc ttcatctatt
     1741 tctaggacag caaacttttt ctgtcctttt tttggttttg cctgcaagaa agtcgataca
     1801 attccctgta ccatattcgc accagtaggg ttggttagaa cttcatcgaa ttgctggcgc
     1861 aaaatattga ctgttaaagc ggtagtcaat gttttaccgt ttgttccggt gacaacgacg
     1921 acttggtagt cttttgccaa actatctagt atatgtggat cgactttcag cgctagttta
     1981 cctggtaaac ttgatccgcc tttaaaaaaa gtttttaatg cccattgcga cgtttttcca
     2041 actgcaatcg caaaatgact gcgtattccc attatgtaac ctccaaaaac gatttgctga
     2101 cgaataaatt caactaatca taccatatgt tgaaaaaaag gttaaattgc ttttcttatt
     2161 cttaagaaaa ggttacggct gaaagtgcat gaaatcggac aaaatcgcat gatttaacaa
     2221 aaaaacagct tgaaacaaaa tgattgtttc aagctgctgt gattttatta ttccctctta
     2281 cctatattcc cattttaacc gataataatt ttctcgcttg gatattcata gcctagatct
     2341 cgtgtttctt ttggcacgaa taacatcaat gtatatagca ttccgatccg cccaataaac
     2401 atcagtgtag caatcgtgat tttgcccaca acagacaagt ctccggtaat ccctaaagat
     2461 aaaccagttg ttccaaacgc agaagttact tcgatgatga tcgagatcag cgtttgttct
     2521 tctgtagcag ataaaaaaac gatacaaaag aaacacatac caagagaaag cataaagaca
     2581 acgactgatt ttcgcacatc atcttgatcg attctccgtc caaaaatatt gatgttatct
     2641 tcacttttga ggaaggaata tagatacaaa ccgatgattg ctacagtagt tgttcgaatc
     2701 ccgccgccta cagaactagg actgcatccg ataaacatca gtaaagaaaa aatgatcagt
     2761 gttgtgattt gaaaatcacc caaatcgtga atctgcagac cagcattacg agtcgtaatc
     2821 gaataaaaca tcgaattgat ccatttgaca ctcatattgg aatcttggaa taaatggtcc
     2881 ttttccaata gataaatcaa gacagttccc gaaacaaaca agataacaaa agctaacaca
     2941 gctaatttgg taaacaagga aaaacgaaac ggaagcttcg cattggaacg tttgtataat
     3001 agccattccc ggcactccat caatacagga aaaccaattc ctccgacaaa aatcaaaaac
     3061 atgatcagga tcaagaaaaa atagtcatgt gcaaagggct tgatggaatc cccagtgacg
     3121 tcaaacccag agttcgtgac agccgatatc gcctgataaa aaccgtaaaa aacggcatca
     3181 cgccaacgat caaaatagcc tctgtaataa aaataaatag aaaagaatgt cccaaaaagt
     3241 aattgaaacc agattaaaat agcaaaagtg atccgaatca gtcggacgat accagacaaa
     3301 cgcggttgat tcatatccgt catgatcagc tgtcgctgtt tcagtgtgat ccgtcgttta
     3361 gaaaaaatga caaaagcagt agaaatcatc atgatcccca aaccaccaac ttgaaataat
     3421 atttccagca gtataatacc gttatcattg aacacagaat tgatatcaaa agtcgatagt
     3481 ccagtcacac tcactgtact gattgccatg aacagcatgt ccacaaaaga aacatggcta
     3541 tctggttcgc gaaaaatcgg catataaaat agaacgagtg ataagaccgt catcaatata
     3601 taataaaaaa cgatgatttg gatcgatgag aaatgattgg cagcaaactt ctgtcctcgc
     3661 cgctgcagcc ttcgaaagaa taaatcaaca tgcacagtac tatcctcctg acaatagtcg
     3721 tttctaccag tatacaaggg gaattaggaa tactcaaaga aattatcaaa cagataacaa
     3781 tacagctttc gattaaaata gaaatggctt ataataagga aaatcctaga aaaaagaagg
     3841 aggaggaata acaaattgat taatgctcaa caaatcgtag aaaaaatgaa aaatcaaaaa
     3901 ataaattatg atacagttct gaaaaaaatg atcatgcaat gggagaaaga agaagtaaag
     3961 cctaagatat taatacatac atgctgtgct ccttgcagta cgcacacatt ggaattcatg
     4021 tctcaatatg cagagatcac gttatttttt tcaaattcga atattcatcc taaaagtgag
     4081 tatttgcgaa gattacatga acaaaaacga tttgtagacc aatttaatga gaaaactgac
     4141 catcatgttg cgctaatcgt tgatgaatac cgtccaaatg aattcaccaa aatggtttta
     4201 acaaaccatc ttgaaaacga aaaagaaggt ggtgctcgct gcagtgcatg cttcaatatg
     4261 cgattagatc ttgttgcgca aaaagcgcaa gaactaggat acgattactt tggaagtgcc
     4321 ctgaccatct cgccaaagaa aaatgcagct ttgatcaatc agatcggaat ggatatccaa
     4381 aagatttacg ggacacaata cttgccaagt gatttcaaga aaaacaaagg ctatgaacgc
     4441 tctttgcaaa tgtgcaaaga gtatgatatt tatcgccaat gttattgtgg ttgcgtgttc
     4501 gctgccaagc agcaaggatg cgatctgaaa gaaatcaatc gggaagcaaa agcttactta
     4561 aaaacatcaa ccgtagtttt tgaggaaaat taaagaaatc tttcggaaaa acgtaagaaa
     4621 aaaataaaga atttatggta gaaataaggt agactgtata atttgtccgt agtaaagtaa
     4681 ggaaacaaga ataaatttca atgaaggaag agataagatg cgaaaagtag cacctatttt
     4741 gaagccaatc gctgtattaa caatctcgac gattgtcgcg actactacag gtattgtgaa
     4801 tagttccagc taccgtgcac caagttcaag ttctagtact gtagaagaaa caacaccaac
     4861 tacttcagtc tcgacatctg aaccaacaac aacggaaagt ttgaatgaaa ccactacgac
     4921 aagtacatgg gacgatacaa caactagttc gtcgtatgat gagacatata caactgacag
     4981 ctcaagcgaa acaagtaata cggtaccaga tggcactcca tcggattcaa ccaatccaag
     5041 tacaagtccg gatacaagtg ctagctccga aacgatacca agcagtacga ctgtagatga
     5101 taacacgcaa aatgatgatg cagcacgtac agcacagcaa aaccaagatt caggaaatac
     5161 ccaaacgtat aaccagcaag caggtgattc tcaatgacat ttttactaga ccttatcaac
     5221 ggggtgcata aattccttgg ctatttagac atcagtccta aatatcttaa ccgtggatat
     5281 acgattttaa gtgtgattcc cacgctttat ttattgagaa ttgtttatgg actttggcaa
     5341 aaccaaaact atcttcaatt tttcttatat ggacttgcat ttctagtgct tgtttatttt
     5401 actgttttaa atgtatttta ttacttttta gataaaaaca cgaaagccga tgtcactcag
     5461 ctttttgtga agtatttacc tgatgaagcg ttcaacatcc agacagaaga atcgatagat
     5521 aatggaaatt cgatcgataa agtgaataca caagaggtca tggtttctta tgacgaagat
     5581 tatcaattga aattagccga gaatatgcgg tatttgattg gaaatggtga gatcaaaaca
     5641 aatgaactcg gtcgcatgga tggatttctg gtcgatagaa atacattgta tccttattac
     5701 tatgtcaaga aaacaggaga gaaagagtat gttctaaata ttggacgaag ctactcggac
     5761 atggaaaaag tagggaccat tcgacttgac tcaacagaag acacattgga acctgtcgga
     5821 ttgttcatcg tcggcggtga ttttgtcaaa gacgggttac gctatcatga gccttatcgt
     5881 ctgaaattga ttgcaaaaaa ggcacagtcg caggaagaac cagtggttta tagtcgtagc
     5941 caaagaagaa gacattcata atgtgatgaa acgccgtgta tcagtggttg cgataaaaac
     6001 gtttattcgg aataaggggg atgtgacacg tcttttgtca cgttcctttt ttatataacg
     6061 agaaagataa gacgaacaca tcacttattc tttcctttca tttataaaca ttgtagaata
     6121 aagaaagtac aacgaagtat tgtgaaaaaa cgtaataaat aacgaggaaa atacaagtag
     6181 gaggtaagct gtgaaagaat ttttgaaaaa ttgggggatt ttgatccttg tattagcggc
     6241 tattttatta gctagagtgt acgtgtttac accagttact gtaaacgggc attcgatgga
     6301 ccctacatta agtgatggac aacgattgat ttcatctaag atctcaaact atgaacgaat
     6361 ggatattatt actacaaagg aaccggggga tgaagaacgg atgattgtta aacggatcat
     6421 cggtatgcct ggagatacag tcaagatgga aaatgaccag ttgaccatta atggcaaaaa
     6481 gtatgatgaa ccttatctgg atgagttcaa aaaagaattt tcagaagata agttacaagg
     6541 agaatatgct tatagctcag gattccaagc acaagcagaa agttcatcca cctttacgag
     6601 tgactttgaa tatactgtgc caaaaggaaa gtatcttgtt ctaggcgata accgcttgat
     6661 ttcaaaagac agccgaatgt ttgggttagt cgacaaagat atgatccaag gaaaagttgt
     6721 tttccgttat tggcctcttt cagaaattaa aatcatgtga tgaaaaaata aaaagaagct
     6781 gtgacatgtt ttttgtcaca gcttcttttt ccgaaaattc cttcttattt cttggagttg
     6841 ctcacttttt ttgcaatctc attttaagct ttttggctga tggataagaa aagcgaagcg
     6901 tttttgttca taaatagctt ttccccacga tggaaggtat gactagtagc agagacaaag
     6961 cccgtttttt ccaatcgatt attcaattct tcttggctaa agccattatg gactttcgga
     7021 tgactaattt gttcattctt gtcaaagtct acgatgatca attgaccgcc aggagctaaa
     7081 atttcataaa gttttgtaag aatattctcc gtatcaggaa tgtgtaaaag tacaagtgat
     7141 agaaggatga gattagcttg gacagcagga aattcgacag aagcatcagc gtggatcgtt
     7201 cggacatttt tcagatctgc tgcttggatc ttttcttctg ccattttcag catagtttct
     7261 gaagcatcgg caatgatcag ttctttgaat ctttccgcaa gcggtaaact aacaagccca
     7321 gttccgcctc cgtaatccag caggactttt tggtggctgt cgcgcggtag ataagtagtt
     7381 agttcttcgc gaataatctt ggctaatgct tgacggtcag gggaatcata atggttagcg
     7441 atggaatcaa aaatattttt tgtcatcaaa tagctcctta cgtaaaactt tgaaatttta
     7501 acttattata gcacgtgaaa agaaagaaag catgctatac tcaattcaaa aagggagaat
     7561 ctagaaagaa tatgccgata tcgtttgtaa aagatagaga ggaaaaagga aagtgtgtcc
     7621 gagaaatatt actagacttg cctgagtggt ttgggcttcc tgaatcaacg gaaaaatata
     7681 tcgaggaatc aagtaaacta cctttatggt gtgaaaaaag aaaagaagaa tatttaggct
     7741 ttatcactct ttcacagaca agtgaagata ctgccgaaat ctattctatt gtatgggaat
     7801 aaaaaaagac tatcatcacc aaggattagg aacaaaactt tttaaagaag cagaaggtta
     7861 cgcagcaaaa cattacaaat atttgcaagt gaagactgta gacgaggggc actactctat
     7921 atatgatcaa actatttgtt tctacgaatc attaggattt tcaaggttgg aagtttttcc
     7981 aaatttatgg gatgaatgga acccatgttt agttcttgtc aaaaaattgg aacaaaagtg
     8041 agcaatagat ttaatactaa tctgtgcttg atgaaaattc tcactgtaaa aaatgctgaa
     8101 aatcaagtat aataccatag gaacaaactg gaaggtgaaa aagagcatgg cacaactatt
     8161 ttttaagtat ggcgcaatga atagcggcaa gacgatcgag atcctaaaag tcgcacataa
     8221 ttatgaagaa caaaataagc cagttgtctt aatgacaagc ggtattgata caagagatgg
     8281 tttaggcgtt gtttcaagcc gaatcggact gaaacgtaat gcgatgccga tatttgaaga
     8341 aacagacgta tttgacgtaa ttcagaaaat ggaaagcaaa ccctattgtg tgctgatcga
     8401 tgaagcgcaa ttcttaaaaa agaaacatgt cattgctttt acgagaattg tggacgaatt
     8461 ggatattccg gttatggcat ttggtttgaa aaatgatttt cgaaacgagt tatttgaagg
     8521 atcaaaatat ttattgcttt atgcagacaa aatcgaagaa atgaaaacaa tctgttggtt
     8581 ctgccacaaa aaagcaatca tgaatttaca ttatattgat ggaaaccctg tttatgaagg
     8641 cgatcaagtg caaattggcg gaaatgaagc ttattacccc gtttgtcgaa aacattactt
     8701 tcacccatca atgattacag aaggagaata agcatgtacg atcaattaca agcaattgaa
     8761 gaccgttatg aagagcttgg agaactttta agtgacccag aagtgatttc tgatacaaag
     8821 cgttttatgc agctttcaaa agaagaagca aacacgcgcg aaacagtcga agtttatcgt
     8881 cgttataaag aagtagtaca aggaatcacc gatacagaag aactgcttgg tgaaaaactg
     8941 gatgatgaaa tggctgaact agcaaaagaa gaattatctg aattaaaaaa agaaaaagaa
     9001 gtcttagaag aaaaaatcaa gatcttgctt ttaccaaaag atccaaatga cgataaaaat
     9061 atcatcatgg aaatccgtgg agcagcaggc ggagacgaag cagcattatt cgctggcgat
     9121 cttttcaata tgtaccaaaa gtatgctgaa gcacaaggct ggaaaacaga agtgatggaa
     9181 gctaacgtta ccggaatcgg tggatataaa gaagtgatca tgatgatcac cggagacaac
     9241 gtatattcga aattaaaata tgaaagtggt gcgcaccgcg tccagcgtgt tccttcaact
     9301 gaatcgcaag ggcgtatcca tacttccacc gcaacagttg tcgtgatgcc agaagctgaa
     9361 gaagtagaga tcgatattga agacaaagat atccgtacag atatctatca tgcttcaggt
     9421 gctggcggac agcacgtcaa taaaacagca tctgctgtac gtttgactca cttaccaaca
     9481 gggatcgttg ttgctatgca agatgaacgt tctcaaatca agaaccgtga aaaagcaatg
     9541 aaaatcttgc gtgctcgtgt gtacgataag atccagcaag aagcacaaag cgagtatgat
     9601 gccaatcgaa aatcagcagt cggaactggg gatcgttcag aacggatccg gacgtataat
     9661 ttcccgcaaa atcgtgtgac tgatcatcgt atcggattaa ctatccaaaa attagatcag
     9721 attttagcag ggaaattaga tgagatcgtt gatgcattga tcatgtatga tcaaacatca
     9781 aaactggaag ctatgcaaaa tgggtaaaat gacctatcga gaagtcctgt cacgggcttc
     9841 ttctttttta gaggatcatg ggaaagaagc atatagtatc caatttttat ttttggaacg
     9901 aaaacaatgg aaaaaacttg attggctcct tcatatgaac gaagagattt cagaagaaga
     9961 acagcggttg atcgaaaccg atctgcgact tctgcttgca gatcatccac ctcagtattt
    10021 gctaggatat gcagattttt atgatcatcg gttaaaggta actgaagcaa cactgatccc
    10081 tcgaccagaa acagaggagt tagtcgaatg gtgtttagac gagacgcctg gtgtaccgtt
    10141 agaagtgata gatattggga cagggactgg agcaattgct attagtttga aagcagcaag
    10201 aaaaaattgg catatatctg ctgtggatct ttcagaagaa gcattagaag tagcgaaaga
    10261 aaatgctcag aaagaaggaa cgaaaatcag tttttatcat ggagatacat taggaccagt
    10321 aatggatcag caatttgatg tgattatttc taatccgccg tacatcagtc ggaatgaatg
    10381 ggaactgatg gatgaaagtg tccggtcatt cgaacctaaa atggcccttt ttgctgagaa
    10441 cgatggatta gctatttatg aaaaaatagc agttgaagca ccaagcgtac taaaatcaaa
    10501 tggaaagatt tttttagaaa tcggatttcg ccaaggtgaa gcagtaaaaa aaatatttca
    10561 gcaagcattt ccagataaaa aagtggcgat aaaaaaggat ctttttggca atgaacgaat
    10621 gatccaagtt tatggttgaa gttgtggatg aaaaattttt tatttgtttt tttattataa
    10681 aaaaacactt atttgacaag gatgtaaacg actttttggt ttatcaacaa gtttatccac
    10741 tattgacaaa tagattatcc acaaacagtg gaaaacttga ggataaactc agacaaaata
    10801 agaatgtatg aaaaaggggt tgtggataaa ctgtggaaac gaaacgatat gatgaaacag
    10861 aattgcaaga ggcagccgaa gcactgaaag aaggagaact tgttgctttt cctaccgaaa
    10921 cagtatacgg attgggcgcc aacgctcttt taccaaacgc tgtcaagaaa gtgttttctg
    10981 taaaagggag accgcaagat aatccattaa tcgtccatgt cgcctctttt gaacaagtaa
    11041 aagagtatgt ggacaacttt catccggcag cagagaagat tgtgaagaac ttctggcctg
    11101 gaccattaac tttgatattt aagatcaaaa aagacacttt gccctcagtt gtgacgggag
    11161 gattatccac agctgcattt cgtataccgg ataacaaaaa aacactagaa gtaatcgaac
    11221 tttccggtgt tccgcttgtc ggtccaagtg caaatacgtc gggaaaacca agtccaacga
    11281 ctgcagatca tgtatatcat gatctacaag gaaaaattac cgggattatt gatgatggag
    11341 ctacgcgaat tggtgtagaa tccactgttc ttgatttaag tgatccaaca gccatgccta
    11401 tgattttacg accaggtgca gtaacgaaag aacaaatcga agcagtcatc gaatctcctg
    11461 ttgctattga tcagcatctt gtaaaagaaa atgaaacacc aaaagcccct gggatgaaat
    11521 acaaacacta ctcacccgat acacgtgttc tgatggtcag agatggcgat tggtcaactg
    11581 cagttcaatg ggcaaaaaat aagaagatcc gtgcaggtgt catagcaagc cccgaaattg
    11641 cggatcaagt gcggacagat actgcagctg tttatatgta taacgacaac tcagtagaag
    11701 cggcggctaa aggattgttt gcaggtctaa gaggattaga tgaaccaaca cttggattag
    11761 atttgatttt tgtacaagtg tatccagaaa ctggattggg gaatgcttac atgaatcgct
    11821 tgaaaaaagc agcaggacaa aattattttg aaaaatgatc aattggttat tgtcgcattc
    11881 ttttagtatg atacaatgct atcaagtaaa tcttctatca gtgaacgaaa aactaagcgt
    11941 aactaatcgt aatagaagag atacgaatat ttggcaattt gtttttgatg ggaaaaataa
    12001 aattgtagga aaataaaaag gagctgttgt ttgtggtaga ttacaaaacg tttgaccctg
    12061 atctttgggc tgcgatcgcc aaagaagaag aaagacaaga acataatttg gagctgattg
    12121 cctctgaaaa ctttgtctcc gaagcagtga tggctgcaca aggaagtatc ttgacaaata
    12181 aatacgcaga aggttatcca gggcatcgct attatggtgg gtgtgaattt gtcgatatcg
    12241 ttgaaaattt agcgattgac cgtgcaaaag aattattcgg agcgaaattt gcgaacgtcc
    12301 agcctcactc cggctctcaa gccaacacgg ctgcttatct cgctttggta gaaccagggg
    12361 acacgatctt aggaatggat ctttccgcag gcggacatct aacacatgga tcaccagtca
    12421 acttcagtgg caaaacctat cactttgttg cttacggtgt tgatccgaca acagaagtta
    12481 ttgattataa tgtggtacgt attttagcta gaaaacatca accaaaattg atcgtagcag
    12541 gtgccagtgc ttacggacga acgatcgact ttgcgaaatt cagagaaatc gcagatgaag
    12601 taggcgcaaa actgatggta gatatggcac atatcgcagg attagtagca gcaggacttc
    12661 atcctaatcc agttccttat gcagatatca caacgactac cactcataaa acattgcgtg
    12721 gccctcgagg cggcatgatt ttgacaaacg atgaagcatt agcaaaaaaa ataaatagtg
    12781 cggtatttcc tggtattcaa ggcggccctc tagagcatgt gattgcgggt aaagcagtag
    12841 cattcaaaga agcattagat cctgcattta aagaatatag tgaacaaatc atcgccaatg
    12901 caaaagcaat ggtcaaagtt ttcaatcaag caattggaac cagagtcatt tcaggggcga
    12961 cagataatca tctgatgttg atcgatgttc gcgagttagg tataaatgga aaagaagcag
    13021 agagcatttt ggacagtgtg aatatcactg taaataaaaa ctcgattcct tttgaaacac
    13081 taagtccatt caaaacaagc ggtattcgaa tcggcacgcc agcaatcact actcgcgggt
    13141 tcaaagaaga agatgctgta aaagtggctg aactagttgt caaagcatta caagcaaaag
    13201 atgataacgc tcaactagac gaagtcaaaa caggtgtacg cgaattaact gaaaaatttc
    13261 cgttgcataa aaaataaaaa tttaaattcc attagtaaac agaaaagttc aatgttgggc
    13321 ttttctgttt attttttggg aaaacggatt ctttgacaat atcggtgatg ctctctagta
    13381 tatgaggtgt aattttagtc ttcacactaa atctcaggga ggaaaagtca tgactgatcg
    13441 tatctcgtta gaaaaggaag cagtagcatt cagtgatgca aatgcgcctc atccgcgtat
    13501 ctatgaacta gcaccggaag aaggaagagt tttactagaa gaagtacaag aatcacctgt
    13561 gaacaaatat tccgtcgata ttgaagactt gatggtggat actggcgaat ggggaaaact
    13621 caatattcgt ctcgtacgac cagaagggaa tatggataaa ttaccagtta ttttttatat
    13681 tcatggagca ggctgggttt ttggcagtgc tcaaacccat gataaattga tcagagaact
    13741 tgcggttcgt acgaattccg tcgttgtctt tccagaatat agtcgatcac cagaagcaaa
    13801 atatccaaca gcaattgaac aaaattatgc tgtgttgcaa cagctgaata aattagccaa
    13861 gtcaaaaaat ctggatctcg agaatctaat cgttgcagga gattcagtag gtggtaacat
    13921 ggctacagtc atgacgatct taacaaaaga acgaaagggc ctcccaatat ccaagcaatt
    13981 gctgtattat ccagtcacaa acgctgattt tgatacagat tcctatcatg aattcggaga
    14041 aaactatttt ttgacaagag aaggaatgat gtggttttgg gaccaatata cgacgaatga
    14101 agaagagcgt gctgaaatca cagcttcgcc tttgagagca aatataaatg aactaaatga
    14161 tcttccttca gcaatgattt taacgggaga agcggatgta ttaagagatg aaggtgaggc
    14221 ttatgcgcgc aaacttcgtg atgcaggggt tcctgttaca caagtacgct tccaaggaat
    14281 catccatgat tttgtcatgg taaatgctat ggatcagaca aaggcaacgc gtgcagcaat
    14341 ggcattatct acttcttgga tcaagaaaaa agaataagct ttgcttgtcc gcaggaaaac
    14401 ttcttttcaa aaacggattt tcttaggaaa aatggcgagt ttgactagtc aatcacttga
    14461 ttttgtttta caattaaaaa gaaacgacag aacaaaggag agcttaaaaa atgggcaaat
    14521 ttcaagtgat cgatcatcca ttgattcaac acaaactaac tattattcgg gacaaaaact
    14581 gcggaacaaa ggtttttcgt gaagtagtcg atgaaatcgc tatgttgatg gcatatgaag
    14641 tatcacggga tatgccattg gaagacatcg tgatcgaaac accgatcact gaaacaacac
    14701 aaaaaacgtt gtctggtaaa aaagtagcga ttattccaat cttgcgcgca ggtattggaa
    14761 tggttgacgg tatcttggaa ttgattccag ctgctaaagt gggacatatc ggtctttatc
    14821 gagatgaaga aacattggaa ccacatgaat atttcgtcaa acttccagaa gacattgatg
    14881 cacgtcagtt gtttgtagta gatccaatgt tggcaacagg cggatcagca atcatggcaa
    14941 tcgatgcatt gaaagaacgt ggcgcgtcga acattaaatt tgtttgtttg gtagcagcac
    15001 ctgagggagt aaaagctttg caagaagcgc atcctgatat tgacatctat acagcttctc
    15061 tagatgaaaa attgaatgaa catggttata tcgttcctgg actaggggat gcgggtgacc
    15121 gtttgtttgg tacaaaataa gtaaatacga aaaagggtgt gacacaactt ttgtcacatc
    15181 cttttttggt catttttaac gtttgatgca gaacagcttt ttcacagctt tcgtttgcat
    15241 ataaaaaaac catcccaaaa atgcagagat ggtcaaattc aaatcttttt tagttctttg
    15301 tagttaacga ttactacacc cttactttgc gtcatcaagt tcgcattttc tgcagtttca
    15361 tcaatctcaa caacagcgga atttttcaat tgctttgaaa tcgttccagt ttgcgacttg
    15421 ccgtgaattg tgaacattac gtgagtattg atcttgaatt tttcaccaat ttccgatggt
    15481 acgatagttg tatcaaattt tccaaaagtt gccattttca taaaacctgc ctttcacatc
    15541 aattatacca cgttttatga aaaatatcat gaaattaaac taattttcca tcggactctt
    15601 tacaatcagc caaatagagt atataatttc ttttggataa ggccagctta gctcagttgg
    15661 tagagcaacg cactcgtaac gcgtaggtcg taggttcgat ccctatagct ggcattaaaa
    15721 gagaagacag atatatcaag gatttttaat ccattgatac atctgtcttt ttgtttaaag
    15781 gaaataaagt atgctttctt agcaatagag catgtattat ttctataaaa atttaggaga
    15841 ataaaacgac tagcaatata gctgtgaaat caaatatcac atgaactgcc caacttgtaa
    15901 tgatcgagtt gcttttaatg gcagcttggt tgaataacaa tcttgccacc ccttgtatca
    15961 aaaggatgtg tgctacagtg tgtaaaatat ttccattatc ataagtactg taatggataa
    16021 atccaaagat gattgctgac aagaaactag cgacaggaat gctgaactta ctagaaaaaa
    16081 tagcgagaaa atacactgag aacaattctt ctccaagtat catgataggc aaaataataa
    16141 ataataacgg gctgaaagcc tcatttattg gattgcctgt taaaccccat ttcaaaattt
    16201 gttgcaaaaa taccgagact gcggcactga ttactacatt gataaaaaaa tatttggcta
    16261 tatttttgac aggagcgaca ggtttcgaga acattttttt cgtatctttt ttaccaaaaa
    16321 caagtgtaaa tgctataaat ccagtgatag ttaaagaaaa aatgctaata atggatggcg
    16381 caaatagcaa ccctaacaaa ccgagtacaa cgacaatagt gcccaatatc ccttttttaa
    16441 gttctatttt ttttcctaaa atgttattca tgataataac tcctactatt cagtttttga
    16501 agatagagtc agtatacatg aagagttaaa
//
LOCUS       DAYYCY010000048        16435 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000048 DAYYCY010000000
VERSION     DAYYCY010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16435
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.048"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(152..550)
                     /locus_tag="ACHAZB_001259"
     CDS             complement(152..550)
                     /locus_tag="ACHAZB_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFM8613945.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(547..1320)
                     /locus_tag="ACHAZB_001260"
     CDS             complement(547..1320)
                     /locus_tag="ACHAZB_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFM8613946.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1317..2000)
                     /locus_tag="ACHAZB_001261"
     CDS             complement(1317..2000)
                     /locus_tag="ACHAZB_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFM8613947.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2167..3054)
                     /locus_tag="ACHAZB_001262"
     CDS             complement(2167..3054)
                     /locus_tag="ACHAZB_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8613948.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3051..3920)
                     /locus_tag="ACHAZB_001263"
     CDS             complement(3051..3920)
                     /locus_tag="ACHAZB_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8613949.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4057..5343)
                     /locus_tag="ACHAZB_001264"
     CDS             complement(4057..5343)
                     /locus_tag="ACHAZB_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8613950.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            5616..6194
                     /locus_tag="ACHAZB_001265"
     CDS             5616..6194
                     /locus_tag="ACHAZB_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM8613951.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPMKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6194..7123
                     /locus_tag="ACHAZB_001266"
     CDS             6194..7123
                     /locus_tag="ACHAZB_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8613952.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7120..8856
                     /locus_tag="ACHAZB_001267"
     CDS             7120..8856
                     /locus_tag="ACHAZB_001267"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM8613953.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(8919..9755)
                     /gene="pheA"
                     /locus_tag="ACHAZB_001268"
     CDS             complement(8919..9755)
                     /gene="pheA"
                     /locus_tag="ACHAZB_001268"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HFM8613954.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(9752..10261)
                     /locus_tag="ACHAZB_001269"
     CDS             complement(9752..10261)
                     /locus_tag="ACHAZB_001269"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFM8613955.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10254..11549)
                     /gene="aroA"
                     /locus_tag="ACHAZB_001270"
     CDS             complement(10254..11549)
                     /gene="aroA"
                     /locus_tag="ACHAZB_001270"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFM8613956.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11562..12653)
                     /locus_tag="ACHAZB_001271"
     CDS             complement(11562..12653)
                     /locus_tag="ACHAZB_001271"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HFM8613957.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(12670..13836)
                     /gene="aroC"
                     /locus_tag="ACHAZB_001272"
     CDS             complement(12670..13836)
                     /gene="aroC"
                     /locus_tag="ACHAZB_001272"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFM8613958.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(13838..14902)
                     /gene="aroB"
                     /locus_tag="ACHAZB_001273"
     CDS             complement(13838..14902)
                     /gene="aroB"
                     /locus_tag="ACHAZB_001273"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFM8613959.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(14935..15957)
                     /gene="aroF"
                     /locus_tag="ACHAZB_001274"
     CDS             complement(14935..15957)
                     /gene="aroF"
                     /locus_tag="ACHAZB_001274"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HFM8613960.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
BASE COUNT         4750 a         3628 c         2919 g         5138 t
ORIGIN      
        1 gcatattttg aaaagaaagt ctgatgctta gagtagaatt tcataaaaat ttactatttc
       61 ataagtattc acgagaaaaa tgcaaacaaa aaagcggaaa taacgctcat ccgccttctt
      121 gtttgtgttt atttcctctt caaacgcttg tctatgattc agacttttct ctatcgttat
      181 tttcatctaa aagttcttgc attccgataa tttctcgata ttgatgaatc ttagtgatac
      241 caatgacttt gtctacatca atactaaaac aaataccatt acctggaata tcgatgttga
      301 tgccttgacc aatcgcatcc attactttat tcgtcaaact atcgggtact aatgtcaaca
      361 caatatcttt ttctggcgaa atgtccaaac ctaaaaattt ccctgaatca tgtacaccag
      421 aacctcttcc atgtaagatc gttccgcctg ctgcacctgc ttcttttgaa aagtcgataa
      481 catcttcgcc cattcctccg tcaacgatcg tcacaatcat gtgaagattc aatgctacaa
      541 gatctttcat ttgctttccc ccaatttttg caatctttct ttttcgattc tgtctttttc
      601 atcttttcgt ttataaatat atcctagtac taaagtggat aatattggtg tcaatgcaat
      661 cattgcaacc acaccaaacc cgtcaatgat cggatctctt ccttcaattt gtgcagcgat
      721 tgccacgctc attgataaaa taaatgtcga acacatcggg cccgttgcta ctccaccgtt
      781 atcaaaagcc attgctaaaa agatcttgtc cacttttcgt cctaaaataa atgctagccc
      841 gtatcctggg atcaagaaat aataaagaga gaatccgacg agcaaacgcc acatagacaa
      901 tcctactgct gcgccgatcc caattgaaat taccatcagc atgattttcg atcggattct
      961 cccctcgctc aattcttcaa cctgtttgat cattacatga atcgccggct cagcaaaccc
     1021 tactaagaac cccattaaaa agccaagggg aaccagtata taattgtaag gcaatcccgc
     1081 aatagcactg cctaaatgct gtcctaccgg tacataagcc atattgactc cgtgcaggaa
     1141 aaggatcaac ccaatagtcg ttatcaagaa acctttcata atgtcaaaga aacgcttttt
     1201 gcgcatacga aaactaatga tattcattaa aacaaatagg attacgatag gtgccactgc
     1261 caaaatgact tccatggtaa cgatttcgat tccatctatc acttgctgcc agaaactcat
     1321 tggaagatca cccccaaaat catcacggca agaatcggcc ctaacgaagc aataccgacc
     1381 attccaaaac tgtcattttc atttttcttt tgtttgatca tactagtcat ccctccggca
     1441 agggccaaga taaatggaac agttactgga ccagtagtca ctccccccga atcaaaagca
     1501 atcggcataa attcggaact ggtgaaaaat gaggcgatca aaacgcctat gtagcctata
     1561 gccatcaatt gataatagga tagcttgaat acgacccgaa gcagcgcaaa ggctagaaat
     1621 actccagtgc cgacgctgac gatcccaatc agcaacactc tgccaatctc tccgtcagat
     1681 atatcatata cttgttgtcc caaaacttgc acggaaggtt cagcaatcgt gatcacgatc
     1741 ccgatagcaa atcccaaact gaccaaaacg gtcaaacttt tctttttgat catatatttc
     1801 cctactagat ctccgacttc catcatagaa taatctgctc caaacaaaaa gagcgtcatt
     1861 ccgatcatca tgattgccgc accaaccaga aatgaagcga ggtcttctcc ttctaacggt
     1921 gcaaaaacaa acgttaaaat gacaatcagg acagtcatcg gcaatatagc agcaatcact
     1981 tctttaaaat tttttagcat gtatcctcct cctttactct ttaagtttac cttaaaatga
     2041 aagagttttc gagcacaaac ttaaaacgtt atcatttaat ttttctcaac ataaaataac
     2101 acacgtccca aaagtatctt tttacttgga aaaatgactt tgaaggaacg cgcttattgt
     2161 gtttttttat tttacagagc cgatcattcc ttgaacgaat tgcttttgca aaacaaaaaa
     2221 tacgatggca gttggtaaag tagcgatgac taatccagcc atgatcattc catagtctgg
     2281 tgtataggaa gcacccatag cagataaaac aagcggaacc gtccgtttat caggagattg
     2341 caaagctact aagggccata aataattgtt ccagctactc atgaatgtga tgattgcagc
     2401 cgcggcatag gtatttttag ccgtcggcat atacactcta aaaaaaatac ttagttcttt
     2461 tagaccgtct aatcgagcgg cttctactag atcttttgga aaagatttaa cattttgtcg
     2521 aaagaagaag atcaaaaaag cagtgctaac agatggcagg atgacaacca agaaactgtt
     2581 gattcccaaa accgttccgt tcaattgtga aaacattcga tataaaggga tcatcaatgc
     2641 cgcaaaagga accatcatcg atagcagtag aatattgaaa attaggtctt ttcgtttact
     2701 ttgataaatc tcaaatccat atcctgccat tgacgaaatc agcaatgcaa acacagtggt
     2761 caccaaagcg atcatcgctg aattccaaag ggaacgagta aaattcagct cattggaaaa
     2821 caaattttga aaattgatga tcaagttact gccaatcttt atttttcccg atgtgatatc
     2881 cacgggtgta ttcgtcattc ccaaaaccat ccagacaaaa gggaaaatgg aaataaatga
     2941 taggatgctt aatgctgtat actttaatac tgacccaatt gtcgaacgaa gttctaatgc
     3001 ttgttttttg acgttggggc gttcgattgt ttctttttct accatatttt tcatcgactt
     3061 tctcctccta ctttcgtttg gattatcgac aatatcacaa taaaggaaac aataacaaag
     3121 gaaactgctg ccgcatatcc aaaattaggc gtgaatttat aactcaaatt atagatgtat
     3181 tgagataggg tggtcgttgc atttgctggt cccccgccag taatattttg tacttcatca
     3241 aaaagctgca atgtcccgat cgtcgaagta atggaagtaa acaaaatgat tggttttaaa
     3301 tttgggatcg tcactttgat aaactgctgc catttagaag caccgtcaat agatgctgct
     3361 tcataaatct ccgaatcaat attttgcatg cctgaaagga aaaaaatcat attgtatcca
     3421 gtccaacgcc aagtgatcga aagaatgatc agaactctcg cccaaattgg atgagtcaac
     3481 cagccaatcg gtgtgtcaat cataccaatg cccaacaaca agttgtttac aaggccagat
     3541 tgagaaaata gacttttcat gatcagagaa taactgacca tcgaagtgat acatggaaga
     3601 aagatagcgg ttcgaaacaa ccctttatat ttcaactttt tatcatttaa aaggttggaa
     3661 ataaaaagtg ctagaaacaa catgatcggt acttgtacaa gtaaatacag gaatgtatta
     3721 aaaaaagctt ttttaaaagt tgcatctgtc aacatccgct gataattacc taacccgtta
     3781 aatgtcatat tgacaggggg accactttga aaagacgtga tcaaagccaa aaccatcgga
     3841 ataaaaacca tgattgatat caaaatgact ggcaagatta aaaaagcatg cccgtttttg
     3901 aataaatctt tctgcttcat tcttttccct ccaattctat cattgattat ctacatgaaa
     3961 agacaagaaa gacaatatct gtaaggcaga cgtgatcttt tttgtctttt cgtttgtctg
     4021 atgaaaaagc gaagaaattc acttttttac caccgatcaa ttagccaatt gtgcttctac
     4081 ttgtttttgt gtatctgcta gtaccttgtc tgtctcttct ccattgatga ttcgatgaag
     4141 agcttcagct acgactgctt caattgcata ggtctcatgt ccgtaattca cttctggtat
     4201 ttcagatgtc catgttgaaa aatcgtcaaa gatcttttga ccactgtaaa attcagaagc
     4261 ttcttgataa gcttcttgtt cattcgcgct aagcatcgta gatactagtc cgatttcttt
     4321 cgcaagagtg cccatcagtt cttcattcga agcaaacgtt gcttctagga aatctttcgc
     4381 tgaatcttct ccagctactc ctttgatcac ataccagcca gcgccgccaa ggttagaagc
     4441 ttgcgctttt tgcagatttt ctggaagtgc aggtattggt gcaatcttcc attttccaga
     4501 ttggttttct gctccttgga tcgttgaaga ataccaagcg ccagttgggc tagaagctac
     4561 tgctcctgat tgtatcgcag ttactccagc attccagtca gaggtttgtt cgactagatc
     4621 ttctttcaaa agagtggcaa acaattcaag accatacttt aaagatgcat tgtcttggat
     4681 cgtgactgtc tccccatctt cagatgtata ccattctcct gcctgttgca taatcatcct
     4741 tactcgacca aggtcagaag gattgacttc agcgattttc tttcccgtct tttctttgac
     4801 atctcgcgct acttgaatat actcgtccca cttcagattc tccatatcct cttctgtata
     4861 acctgcttct gccatcagat ccgtacgata gaagtttgct gttacgcccg aatcgaaagg
     4921 aacaccgtat attttatctc ctactttgtt taccgcaaat ttatacggtg caaaatgatc
     4981 ttcctttaca atagcagaca aattagcgaa tgcatctgga taagaagtca aatagccttg
     5041 tatcctataa tcttcaatca agacgatatt gggcaatcct tctgtattcc cgctcgctaa
     5101 agcggtgttc agtttctgaa cgatatcatc ctgagacatc gtaacaactt ctatctctgt
     5161 ttcttcgttc tcataaactt tttttgcttc attcactgct ttgatattga atgtctcatc
     5221 ccacgcccag acagtgactt tttcttcttt gtctgctgta ctactagcac ttccgcatcc
     5281 tgttaagaaa cctgatgctg ccgctaaaca aacgactcca gcaattaact ttctgatgtt
     5341 catttttatc cgctcctttt ccatccatta gttgtttacg tttgctacat tcatcatacc
     5401 aaaatttgaa aacggtttct ttattattca gatacttcaa aaatttctcc taaaataata
     5461 aaaaaataca aatcaactat gacaaaagga ataatcttag catctagacc aaagatcgaa
     5521 atggtgaggt ttctttaaaa taaaaatatc cttgcatttt ctcctagaaa taagtatttt
     5581 taaattacaa gactattctt aaggcgggta aaaaaatgcc aatacgcgta attcttatcg
     5641 atgatgaaca gacgatttta gatggaatga aacagttgat caattggtct gagcacgggt
     5701 ttcaattaat agggactttt tccagtccga tgaaagcgct tgatttttct ttggtacatg
     5761 caatcgatat cgtggtcaca gatgtaatga tgcctgaatt aaatggaatc gaattatcgc
     5821 ggctattaaa acagcaacaa cctgaaacac agatccttat cctctctagc tatgatgaat
     5881 ttgatatggt taaagattct tttaaagaag gcgtcgctga ttatctttta aagcctcgat
     5941 tgagtcctga tttttttcta aattccttaa ctgccgtttc acaaaaaatc aaaaaaaagc
     6001 ccaacaaaag cccaatcacc aatcgttatg agcagatctg cgagaatctt tcacagcaga
     6061 ttgctggaca aactcgtcca ttgcttgacc ctcttctttt tgaacacacc ttgtttttct
     6121 gttatatacc gaaaaacggc cattcagttc aaaacaaggc tctaaacaaa aacagtcgat
     6181 cacccattca ttgatggata gcgacgattc gtttactatt tatcctgttt ctaccttttc
     6241 ggaagaatcc ggctatgtaa tcaatactga ttccaaagat acacttagga agtttgttca
     6301 aaagtttcgt caagaagttt ttccaggtga tttttttgtt gtttctgatt cttttgcaat
     6361 ccaagatatc aatgaaatat tccaagagat cagaaattgc accaaaggac aacgtttcta
     6421 tcataaggga aagaaccttg cttatcagca tgaacttctt ccgttattaa ccaaccacga
     6481 gcagcaagca agtagttatt taacaaaaat cgttcataaa gactacgctt tagctgtcaa
     6541 agaacttgag aatttcttca cgacgatatt ggaacagcct attgatccag cttatttgaa
     6601 acatgaggca atcaatcgtt tctatgcatt actcaacgct ttggctgacg agcatgttgg
     6661 gaatgaagaa ctttctttgc tcaagatcgc ccttccttct ttattagccg atgctgagta
     6721 tttcgaagat ttttctattc ttgtcaaaga aacacttgag aaactcaccc acttttctgc
     6781 aaatgcaaaa aaagacaatt tcgatatctt gactttgatc tacgagttcg tccaggaaaa
     6841 ttatgagaaa gaaatcaatt tacaactttt atccgaaaaa tatcattttt catatagcta
     6901 tctctcagcc atctttactg aaaaatttgg tatcaacttt tcaaaatatc taaaaaaagt
     6961 ccgtatcagt aaagccaagc tctttttgac ggaaacacgg cttactttga cggaaatctg
     7021 tgagaaagtc ggctataccg aactgggtta tttctccaga gtttttaaag aagagacagg
     7081 aatgacacct tctcaatacc gtaaaggaac ttttgtaaaa tgagaaaaaa aatcaaagag
     7141 catgaactct ttgccaaact tctgttcatt ttgttcatcg gtctttttct gcaagccatc
     7201 gtcatttctt tgtttattta tcatcgatca agggatgcct atatccagct gttcaatcaa
     7261 tcgaatgatg tggtactaaa aaagattcaa tctgaatttg aaagtttgaa tgatacaatc
     7321 gagaatacat tagccgcttt tgattctaat ccagctgtca aaagctattt ttccaatgat
     7381 ccagcccaac atatggaaca gcttcagcaa ctaagaacaa tccaaaaaat gaatgactct
     7441 ctttcaaaaa tccatccaat gatcgattac gatgtattga tctttggaga aaatggaaga
     7501 acttttgtcg gaaacgacat gctcactgcc gtatctgccg attctttttt tcaatctgcg
     7561 attgctcaga gagtgaatga acgtgctgct gatacacaaa tgctttttgc acaccacgga
     7621 ttgactcttc gcgataagaa agcacccagc gtcttctttg tcaaaaaact gaaaaataca
     7681 ttgaatcacg tttacggata tgctgtttta tcgatctcat ccaagcaact agcgaatctt
     7741 tttcaatctg tagtaaatcc tgagatatcg aagatctcct tcatgaacga agaacagacg
     7801 atcatcgcat caaatgaaga aaatacaatt gggagccgat caatgatttt caaaaaacta
     7861 acagtaggtg aaacaacgat ttcaaatgga cagcagttga cccgtcttcc tctgtaccgc
     7921 caggattgta cattactcag tcaagtcgat cttcattctt tatctaacca aatgggcgtg
     7981 atttttccga ttattttgta taatatcgtg acacttgctt ttgctggctc attggtattt
     8041 atgtatttgg atcgacacac aaaatcgatc taccgcttga ttgattcgct aaaacggcta
     8101 gaggaagatg gcttaaaagc tactgttcct gtacaaggaa gccatgaagt ccaattgcta
     8161 gggaatacga tcaatcaatt gctaagtaat gctcagcatc attatgaaca gacgatccaa
     8221 gacgaaaaga agaaacgttc gcttgaaatc caaactatgc aagcacagat acagccccat
     8281 tttatttata atacgctgac tagcttgaaa tttttgattt ggcagaaaaa aacagatgaa
     8341 gcagttcaag gaatcgacag tttcattact ttactaagaa gtacgattgg gaaaaaagaa
     8401 gaagtgattc cagtcaagga agaaatcaaa agcgtacaaa gttacatcga tcttttgagt
     8461 atgcgatatg gcgaaagaat ctcggctaaa ataatgattc ctgatgaact gcagtcatta
     8521 tctatgccga atatgatcct tcaaccaatt atcgaaaacg cctatcttca tgcttttcac
     8581 aataaaacgc aaggattcat cacggtgtac ggcaaaacaa aaaatcagct tcttttattc
     8641 gaagtgattg ataacggcga tggcttcgat actgaaaata ccaaacaaaa aaatgatcat
     8701 ttttcaggaa tcggcatcag ccatgtagat gagcggatac gcctaatgta tggcgatgca
     8761 tatggcttaa cgatccaatc agtgattggg acaggaacga ctgtaacgat ttgtctaccg
     8821 cttcttccta aaaatcaatc agaatatcaa aaataaagaa tagccaagcg ctgatttttt
     8881 tattttaagt taaataaatc aatcagtatt cttgactttc atctacgcgt ctcttctact
     8941 gtcagcactg gataacagcc caatatttga ctttctcctc cgagcatctt gatttcttgg
     9001 agcgcattct ctactaatgt cattggacgg tccaacaaca aatcgatcac gaaaaaatat
     9061 tctcccaagc tagttttcaa tggtcgggat tcaatcttac ttaagttgat ttcccgccag
     9121 ccaaaagctg ccagcatttt atgaagcatc cctggccgat tagcaggtag tgtcaagatg
     9181 acactcattt ttactggagc tgactgctgg ctggtcatct ttcggtcacc gacgatccaa
     9241 aaacgtgtct ggttcaactc attatcttgg atattctcag ctaagatctc caatccaaca
     9301 tgctgtgctg tttctaatga agcaatcgct gctgcgtctt ctttcggatg ttctgctaca
     9361 tacattgctg cagcagttgt tgattctgtt gctactaaag gaacattagg ataatgcgtt
     9421 tctaaaaact gttgtgattg tgctagtgct tgtggatgtg aaaggatttt tgtaatcttt
     9481 gttgctggat tgcctaaaag ctgctgcttg ataggaagaa cgatttcact tttaacttcg
     9541 acttctggat gtttggaaag aagatcgatt gtgtgatgaa cagctccttc caatgaattc
     9601 tctacaggaa cgacagccag atcgacttgt ttgcgaaaca aagcgcgcaa acaaacaggg
     9661 atggaagcat acgcagtcaa ttgttctgta ggaaatagct gacaagccgc ctgataagtg
     9721 aatgaacttt ctggccctag atagctgacg ttcatatgct tttgatcctt tctataatgg
     9781 tatcgatgat cttttctggt gtttgattgg tcgtgttgat tcgatgtgtc gcactttctt
     9841 cataccaaga aagtcgttgt gccaataatg cttttatttc ttctcttgtt tttccaagag
     9901 ctaaaggtcg aatagacgtt tcatcttgtt ggatacgatc aactagacag tccgcttctg
     9961 cctctaagta aagaacaatc ggctgtgttt ttaaaaaacg ccgattttct gcactttgga
    10021 caatccctcc accagtcgcg ataattttgt cttcatttga cagctttctt aaaatatctg
    10081 actccacttt tcgaaagctt ttttcaccgt aatagtgaaa atattcggca atcgacaatt
    10141 ggatatgttc ctcaatcttt gtatctaaat ctacatactc tttttgtaaa cattgagcta
    10201 accctttacc gattgttgtt tttccagcgc ccatgaaacc aatcagcaaa atgctagcca
    10261 tgagcttctc ctcctgacac aagttctgct aaatcgtcaa aaaatgccgg ataagagatt
    10321 gaaacagctt cagctccttc tagttcgcaa ggtccatcag ctatcaaagc agctacttgc
    10381 agcatcatac cgattcgatg atcgcctctg ctggacactt ttgcgccatg cagaggtgtc
    10441 ggcccatgga tgatcagacc gtctgctgtc gcttttatct ttgctcccat tttctgtaat
    10501 tcttctgcca ccgcatcaat tctgttggtt tcttttactt ttaattcttc tgcgtctttg
    10561 atgatcgtga ttccttctgc ttgagtagcc actaaagcta gaatgggcaa ttcatcaatc
    10621 agacgcggaa tgatttctcc accaatcgtt gctttcttca aatgactcgt cttgacactt
    10681 aaatcagccg attggttctg ttcattgtga ttcgtctggg taatcgctgc gcccatctcc
    10741 tccaacacat ctaagatacc agttcttgtt ggattgaccc cgacattttt taacaacagt
    10801 tggctttccg gaacaagaag tcctgctgct agaaagaaag cagctgatga aatatcacct
    10861 ggaacagtta tctgttgacc agttaatttt tgcggtccgg tcactatgat cgttttatct
    10921 tccactgtga tccttccgcc aaattgacga atcatttctt ccgtatggtt acgggaacgt
    10981 tctttttcaa cgattacgga tgtgccttct gcttgtaatg ccgcaaacag caaagcagat
    11041 ttgacttgcg cactggctac aggcatattg tatcggatag gtgataaaga gcgggttcct
    11101 gtcacagtga ttggtagata ctggtcattt tcttctcctt gcaagtcagc gcccatctcg
    11161 cgtaaaggtt ccatcactcg ccccattgga cgtttagaca acgaagcatc cccgaacaga
    11221 gtagtcgtaa aaggctgtcc agccaaaata cccatcaata aacgtatggt cgtaccggag
    11281 tttcccatgt caattgattc ggttggttgg acaaatgagt ggctaccgcg accatgaatc
    11341 ttgatcacct cttcctcttc ttcgatctcg acacctaatt gttggaaggc atgtaaagta
    11401 ctcagacaat cttctgcgcg caaaaaattt tgtatagtcg tcgttccttc agcaattgct
    11461 ccaaacatga tgctgcgatg ggaaattgat ttatctgctg gtatcctaac agtccctctt
    11521 aatccatgta tttgttgtaa taattgcatt tatccagcct cctatttgat ccagcaatga
    11581 taactggtat tagacataat gattcttttt gcttgtaaca aatcctgttg gtttttaaaa
    11641 ctgatttgca gtattcctaa aatatcttcc ctggtctctt ggatcttcaa attaatcaaa
    11701 gaaactcccg cttttccgat cagtccagtt acttctgcaa taacgccagg ttcatctggc
    11761 acatcgataa ataaatcaaa aaaggcagga atcgcgcctt gcttatggac aggcaactgg
    11821 tctcgacttt cttttgcccg attgaaaaaa gaaaagattt ccgattggtt ctcagtcatg
    11881 atccattctg ctatttgtgt catttcttgt tgccaatctg ccaatcgttc caataatatc
    11941 tgcttattgc tcaacaaaat atctgtccac atgacaggat ctgaggaagc gatcctagtg
    12001 atatctcgaa agcctcctgc tgccagttgt ttggctcgcg gatactcttg attgaattgt
    12061 tctgcctgat tgactaagcc agctgccaga atatgtggaa aatgacttag cattcctgtt
    12121 atacggtcat gttctcttgc tgtaagagtt acatattttg ctttggttcc gcaaaatagt
    12181 tcttgcagtt tttcaacatc ttttttcgtc ttttccaaag gtgtaaagat gtaataggca
    12241 ttttcaaata gtttagcatc tgctgcctgt atacctgatt tatgagaacc agccattgga
    12301 tgtccaccga caaaccgaag atttttctgc tcagcggatg tcacgacttg ctgcttcgta
    12361 ctgctgacat ccgtaatgat gacgtgatca gacaagttga ttctttcaag gacatcaaga
    12421 taagctaagg ttgtacggac gggaacggct aataagataa cgtctgcttg ttctgcacct
    12481 gtttcaaaat cacttgcaac gtaatcaacg atctgctgtt ctactgcttg ctcacgggtt
    12541 tttgactgac tatcccaacc aactactatc agttctggat tggtctgacg aatacaaagt
    12601 gccatagaag caccaatcag tccaagcccc acgataaaaa catgttgtgc catcttattt
    12661 gccccctttt tagtaatgtt tcaagtagtc acggtactcc ttcactgcag ctttcaactc
    12721 aacaagcgaa tcacttggga attttgacaa tagctctgtc gctaccacag tggctacaac
    12781 attttccgca acgacgctag ctgcaggaac tgctgtacta tccgaacgtt ctacacttgc
    12841 tttgtaaggt tcttttgtat cgatatcaac actttgaagt ggcttgtaca atgtaggaat
    12901 gggtttcatg accccgcgaa tgacaagtgg ttcgccattg gtcattccgc cttcgaagcc
    12961 gcctagatta ttggtacgtc ggaaatagcc tctttcttct tcccatagga tctcatccat
    13021 cacttctgag cctggtaatg aaccagaagt gaatccgacc ccaaattcta cgcctttaaa
    13081 ggcattgata ctaaccacag cttgtgcgat tttggcatct agcttcgtgt cccattgtac
    13141 gtagcttcct aatccagctg gaacgcctcc aacgagcact tctacaattc ctccgatcgt
    13201 atctccattt ttctttgtct gatcgatcaa atcttttatt ctttgctcaa ctgctggatc
    13261 aacaactcgt acttctgacg cttcagaaat ttctttgatt tcttgaatcg agatatttcc
    13321 aggtatttct gcttggattc caccaagaca aatcacgtgt cctgctacga tgatgtcaag
    13381 ttcttccaga attttcttgg caattgcgcc gattgctaca cgcatggtcg tctctcttgc
    13441 agaggaccgt tcaagcacat tccgaagatc tcgatgatgg tatttcatac cgcctaccaa
    13501 atccgcgtgt cctggacgcg gacgtgcaac tcgccgcagt gattggtctt tttcaggcac
    13561 ttctgctaca gacatgacag ttgtccaatt tttccaatct ttattttcta ccactaaagt
    13621 gacaggtgaa ccaattgttt tgccgtgacg gatgccagac gtgatccgaa cacgatcttt
    13681 ttcgatcttc atccttcctc ctctgccgta accagcttgt ctgcgagcga gttcgtgatt
    13741 gatttcttct tctgacagag gtaatcctgc tggtagtcct tcaataatgg ctgttaattc
    13801 tggtccatgg gattctcccg cagtcatata acgcatctta cagtcctcct aagtaatctt
    13861 tgaatgtctc tgtgggaaca ggaacgattt ttgcttgccc aatcttttct agcaagatga
    13921 tattcaattg gcttcctctt gctttcttat catgggttat cgcgtggaat aattcatctg
    13981 gatgacttag atcgtacgta atcggcagat ggaatttttg aagtatcctt tctaaagcag
    14041 ctgtcgatcc agttggagac aagccgattt tttctgcatg agaagtgatg gctaccattc
    14101 caatagctac tccttctcca tgactgacta ccccatatcc tgctgtattt tcaattgcat
    14161 ggccaattgt atgaccaaaa tttaaaataa gtcgatttcc ttggtcaaag acatcttctt
    14221 ccactacttt tcgtttgatt tccaacgtag cagtaatgat tggaacagca tgttccagaa
    14281 gatctttttc gtctttcagc tcgttcagct gtttccaaag cgttgcatct gctatagctg
    14341 ctgatttgat gatttcagcg attccttctc gtaaacgacg taccggcaaa gtttttagaa
    14401 cctcaggatc aatcaataca ccagctggct ggctaaatgt tccgacaagg tttttcgctt
    14461 tgtttgtatt gactgctgtc ttcccgccaa tactagaatc tacttgtgca agtagcgtag
    14521 tcggaatttg aacgaaatgg atcccacgca tgaacgtcga ggccacgaat gctgctagat
    14581 cacccaccat tcctccacca agtgcaagta tcccatcgct gcgggtaaaa tcattatctg
    14641 ctaaaaaatc gtataactca gctgcttgtt ctaaagattt actggtctca cccgccggta
    14701 cgattgaaag gactggttca aaattagctt gtctcaattg gaacatgatt ttttctgcat
    14761 atagtggagc aacgtttgta tcagtgatca cagctacttt ttgaggtctc caaatcgttt
    14821 gaagccatga tccagcctgt tctagtgcgc ctttttcaat atgaatatcg tatttttggt
    14881 tatcaacatg tacagtaagc attcgtttca cttctttctt ttaaaaagct ggaattaatc
    14941 gagtgtctga atcttttcca ttgcttcttt catgatcgca acttctttca tcatgcgtga
    15001 gtaagtcttt tcattcaacg attggggacc gtctgaccaa gcatttgcag gatcaggatg
    15061 gatctctacg atcatgccat ctgctccgct tgccacaccc gcacgagcca ttggtggtac
    15121 aagttcccaa attcctgtac catgacttgg atcaacaatc acagggaaat gagttaattt
    15181 tttcatcaat gggacagcac ttaaatcaaa cgtattacgt gtcgctgttt cgtaagtccg
    15241 aatcccacgt tcgataaaaa tgacaggaga tttgtcttcg actgcaatgt attctgcagc
    15301 attaagccat tcattgatcg taccggaaat tccacgtttc aatccaacag gtttacctgt
    15361 tttaccgaca gcagatagca gtttaaaatt ttgcatatta cgtgcgccaa tctgtaggat
    15421 atcgctgtac tcagccacca tatcgatatg cccttcatcc atcacttcag taatcacttt
    15481 cattccaaat tcatcagccg cttgacggat gtatttcaat ccttcttctt ctaaaccttg
    15541 gaaagcatat ggagaagtac gtggtttgaa ggcaccacct cgcagaattt ttgcgccccc
    15601 ggctttggcc attcgcgcac attctctgat ttggtctaat ccttcgatag aacaaggacc
    15661 agccatcgtc acgaaactgc cgtcaccaat ttttactccg tctacatcca cgactgtatt
    15721 gccaggatgg aattctcgag aagtcaattt gtatgtgttt aaaatacgca cagcattttc
    15781 tactccttca tagcgtgtaa atgctccttc ttgtaaactt cgcgtatcac cttttagtcc
    15841 aatcactact cgttctgttc cttgatcgat tgagactttg aatccttctt tttcgatatg
    15901 atcgacgact ttttttattt gttcttccgt tgcttctttt ttcataatta ctatcattgt
    15961 ttttcccgct ttcttttgtt tacttatcta tcttggctga ttttctctac tttttcaagg
    16021 tggttttaca ttaattccta ctaaatgacc gtgtcctgtt gttctcattt tcgctccttc
    16081 tttcctactt atgacccaga taatcaacaa aaaaagatgt ccctgtagga aattgcctct
    16141 acagtgaaca tcttcaacga gccttaaaaa gcgctgcaaa aatataaaat agccactata
    16201 gatgaatttc tctacgtggc tgtaagtcgt gtttaaaaat ctttagccat catagataca
    16261 aacctggttt gtttgtaccc acgaagtcat gaatacaaac ctatctaaaa tagctaaagt
    16321 aatacgtatt cagttttgta agttttctag atttcataaa aaatcgctcc aaactgatgt
    16381 aagaattgta cctagtatag acataaaaaa atcgactgtc aatcattttt atgaa
//
LOCUS       DAYYCY010000049        16362 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000049 DAYYCY010000000
VERSION     DAYYCY010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.049"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            3..551
                     /gene="hslV"
                     /locus_tag="ACHAZB_001275"
     CDS             3..551
                     /gene="hslV"
                     /locus_tag="ACHAZB_001275"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslVU peptidase proteolytic subunit"
                     /protein_id="HFM8613961.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            565..1965
                     /gene="hslU"
                     /locus_tag="ACHAZB_001276"
     CDS             565..1965
                     /gene="hslU"
                     /locus_tag="ACHAZB_001276"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HFM8613962.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            1983..2774
                     /gene="codY"
                     /locus_tag="ACHAZB_001277"
     CDS             1983..2774
                     /gene="codY"
                     /locus_tag="ACHAZB_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HFM8613963.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            2856..3731
                     /locus_tag="ACHAZB_001278"
     CDS             2856..3731
                     /locus_tag="ACHAZB_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFM8613964.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(3863..4501)
                     /gene="plsY"
                     /locus_tag="ACHAZB_001279"
     CDS             complement(3863..4501)
                     /gene="plsY"
                     /locus_tag="ACHAZB_001279"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFM8613965.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            4628..5062
                     /locus_tag="ACHAZB_001280"
     CDS             4628..5062
                     /locus_tag="ACHAZB_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFM8613966.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            5228..7273
                     /gene="parE"
                     /locus_tag="ACHAZB_001281"
     CDS             5228..7273
                     /gene="parE"
                     /locus_tag="ACHAZB_001281"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFM8613967.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            7285..9735
                     /gene="parC"
                     /locus_tag="ACHAZB_001282"
     CDS             7285..9735
                     /gene="parC"
                     /locus_tag="ACHAZB_001282"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFM8613968.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            10012..12243
                     /gene="pflB"
                     /locus_tag="ACHAZB_001283"
     CDS             10012..12243
                     /gene="pflB"
                     /locus_tag="ACHAZB_001283"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFM8613969.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            12487..13248
                     /gene="pflA"
                     /locus_tag="ACHAZB_001284"
     CDS             12487..13248
                     /gene="pflA"
                     /locus_tag="ACHAZB_001284"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HFM8613970.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            13333..14259
                     /locus_tag="ACHAZB_001285"
     CDS             13333..14259
                     /locus_tag="ACHAZB_001285"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HFM8613971.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            14441..15334
                     /locus_tag="ACHAZB_001286"
     CDS             14441..15334
                     /locus_tag="ACHAZB_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HFM8613972.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            15468..15593
                     /locus_tag="ACHAZB_001287"
     CDS             15468..15593
                     /locus_tag="ACHAZB_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HFM8613973.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(15727..16359)
                     /locus_tag="ACHAZB_001288"
     CDS             complement(15727..16359)
                     /locus_tag="ACHAZB_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8613974.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
BASE COUNT         5810 a         2788 c         3492 g         4272 t
ORIGIN      
        1 acatggtcga atcaccattt cattcaacca ctatctgtgc cgtcgaaaaa gacgggaaat
       61 ttgctatggc tggagatggc caagtaacaa tgggtgaatc tgttgtcatg aaaggcactg
      121 caaagaaagt acgacgtatc tacaatggag aagttgttgt aggtttcgcc ggaagtgttg
      181 cagatgcgtt tactttagaa gaaaaatttg aaggcaaact aaacgaatat aacgggaatc
      241 tacaacgcgc agctgtcgaa ttagctcaag aatggcggac acagcaatct atgcaaaaac
      301 ttgaagcgat gctgatcgta atgaatgata aagaaatgct tcttgtttca ggtacaggag
      361 aagttatcac accagatgat ggtatcttag ctatcggatc aggagggaat tttgcgttgg
      421 ctgcagcccg agcaatgaaa aactatgctc aaaacgacat gagtgcgaaa gaaattgcag
      481 aaaatgcgtt aaatattgcc gcagatatct gtatctttac taatcataat attatcgtag
      541 aagaactgta agaggtgagt agatatgtat gaattaaaac aaacaccaag acaaatcgta
      601 agtgaattag atgaatatat cgtaggacag cacgcagcaa aaaaatctgt tgccgttgct
      661 ttgcgtaacc gctatcgccg tttgcagtta gatgaaaaaa tgcaacaaga tattacacca
      721 aaaaatatgc tgatgatcgg acctacaggg gtcggtaaaa cagaaatcgc acgacgtttg
      781 gcaaaaatcg tcaatgcacc atttgtcaaa gtcgaagcaa caaaatttac tgaagtcggt
      841 tatgtcggaa gagatgtaga atcaatggtt cgggatttag tagaaaatgc gatccaaatc
      901 gtagaaaaag agcagtacag ccgtgtatac acaagtgcta aaaaaaatgc agaaaaacgt
      961 ttggtaaaaa tcttggtacc tggtatcaaa aaagaacaga aacaatccac taatcaattt
     1021 gaacaaatga tgaatctttt taatgctagt cagcaaaata ccgctgaacc gcaagaagaa
     1081 gtgacagaag atattcgaat caatcgccgt acagtcttgg aacaactaca aaagggttta
     1141 ctggatagtc gtgaagtaac cattgaagtt gaagaccaaa agaaacctgc accaatgatg
     1201 aacaatggat tggagcaaat ggggatcgac ctaaatgaaa ctctaggtgc tttgacaccg
     1261 aagaaaaaag tggaacggac ggtaacagtc aaagaagctt tagagttatt gatcaaggaa
     1321 gaatcagcaa aattagtcaa ggatgctgat atccatagtg aggcaatcaa attagcggaa
     1381 gaaagtggaa ttatttttat cgatgaaatc gataagatca cttctaaatc acagcaatct
     1441 ggagaagtat ccagagaagg cgtccaaaga gacatattgc caattgtcga aggttcacaa
     1501 gtaaacacaa aatacgggat catccaaaca gatcacgtcc tattcattgc ttcaggagct
     1561 ttccatttgt ctaaaccaag tgatctaatt ccagaacttc aaggacgctt cccgatacgg
     1621 gtagaattag atgatttgac agctgaagat tttgtgaaga ttctgaccga accaaataat
     1681 gctttagtaa aacaatacgt cgcgttgatc ggtacagaaa atgtgtcagt tacgtttaca
     1741 aaagaagcaa ttgaacggat cgctcaaatc gctttcgatg tcaaccgtga cacagataat
     1801 atcggcgcac gcagactgca taccattttg gaaaaactac tagaagatct attatttgaa
     1861 gcaccagata tgcaaatggg agaaataacg atcacagaag catatgtgga tgacaaactt
     1921 ggctcaattg cggcaaatga agatttaagt cgctatattt tataaaaaat cggaggacga
     1981 caatgacaga cttgctaacg aaaacccgca agataaacac acttttgcag caaaagaata
     2041 cttttgacat gcaagcagat ttgccttacg ataaaatggc tgtcacacta ggtggtattc
     2101 ttgacagcaa cacctatatc ataggaagtg acggagtatt actaggttat aatgaacgtc
     2161 acgatgtgaa caatgaacga atcaaaacaa tgtttgttga gaaacaattt cctgaatctt
     2221 atactgatac agtggcacag ttgatgaaaa ctgaagctaa tatcccaatt actagtgata
     2281 ttactgcatt tccggtagag tcaagagaga aatatccatt tggtctgacg actgtcgttc
     2341 cgatctttgg agctggcgaa cgtttgggta cgattatctt atctcgtatg gaacaatcgt
     2401 ttgcagatga cgatctagtc ttagcagaat atggagctac ggttgtcggt atgcagatcc
     2461 tttatcagaa gtctcgaagc atcgaagcag atgtacggag tgcaacggct gtccaaatgg
     2521 caatcaacac attatcttat agcgaattga aggctgtaca agctatcttt gaagcattgg
     2581 atggagatga aggtcgtctg actgcttcta atattgccga tagtatcggc atcactcgtt
     2641 ctgtgatcgt caatgcttta cgaaaattgg aatcagctgg aatcattgaa tctcgttcgt
     2701 taggcatgaa gggaacttat ttaaaagtat tgaattcccg ttttaaagac gagttagcaa
     2761 aacacagtta ttagaagtaa ggggactggg gcagatagaa atgtttgttc cagccttccc
     2821 attttaaaaa gtaagtcgaa gttggaggaa tggcaatgac tgttattatt caaaatgacc
     2881 agctgatcgc cgaaatcgct gaacacggtg cggaattgat cagtctgaaa tcaaaagaac
     2941 atggtttaga gtatatctgg caaggtgatc cagcgtattg gggcagacat gcaccggttt
     3001 tattcccttt tgttggacgt ttaaaagatg atcagtatgt ttatcaaggg aaaacatatc
     3061 aaatggggca acacggattt gctcgggata tggattttga agtcattgaa catggacaag
     3121 aaatggctag tttcatctta aggagtacgc cggagacatt agaaaagtat ccatttgatt
     3181 ttgaattagt gatcagctat gaattaggag gagacggcat cacggttcat tatcaagtgg
     3241 caaataccgg tgaaaaagaa atgttctttt ctatcggtgg acatcctgct ttcaatgtac
     3301 ctcttgaaga aggattgtca tttgaagatt tctatctggc attttctcca ttgaaatcaa
     3361 gattacgcct accattgaaa ggcccattca tcgatatgga tcaaaagacg ttaggtcaga
     3421 caaatacgaa tctggctttg aatcgtgaac tttttgagca agatgccttg gtttttgaaa
     3481 caaggggtct aaatgcgtac gccattcgaa gtgaaaaatc ttcccatagc gtaactttaa
     3541 gctataaaga tatgccttat gtagggatct ggtctcctta tccaaaagaa gcgcctttcg
     3601 tttgtatcga accgtggtgc ggaatcgctg acacggtgaa cagtagtgga aacttgatgg
     3661 aaaaagtagg gatcaatcaa ctaggtgccc atgaaatctt caagacaaaa tattccatca
     3721 cagtgaagta agaggttgtg accgagaagt tagcagggaa caccgtttac caaggtgtgt
     3781 aaaaaagcta tcctgtaagt aataagggag tgtgacatcg atttgtcaca ctcccgtttt
     3841 atctctttgg aatgaaaaag tatcaggatt ttttcttgct gctattcaaa ccaaacggga
     3901 ttcgactttc agtatggttt ctgatacgct tgatattgtc tttatgccga taaaagataa
     3961 aggaagaaac taaaaaggca atgatcgtca gtaaccaatt gggcttagcc aaaatcacag
     4021 gagctacata aggaaggacg atcgtagata aagtgatcaa tactgcactg atcatactgc
     4081 tcaaactcac catacttgta caaaataaac taacgataaa gatgatacaa gaataaacaa
     4141 aaaatacagg attaaatcct aaaagcattc ctgcacttgt cgcaacagct tttcctcctt
     4201 tgaacccagc aaaaatcgga aatgtatggc caagaatggc gcatacccca aaccaaagag
     4261 ggttaatccc agaaaggtgg aagatcatcg gcaaacaagt agccaatgta ccttttaaaa
     4321 tatccataag caggacaaca atcccggctg ttttgcctaa tacacgaaat gtattggtcg
     4381 ttccagtatt accgctcccg tgttgtcgga tatctttttt gaaaaacaat ttgccaatcc
     4441 agacaccgga aggaatagaa cctaaaagat atgctaaaat aagtaataag atcaatttca
     4501 ttttttaccc tctttcaagt tgaaaactat cctttatttt agcatgaata ggtaacagga
     4561 acaattgaaa aaatgaaaga tacaagtata atagatggaa taaaaagaaa agcaaggggt
     4621 ggcagaaatg tctgtaaaaa atccaacaca agaaacgatt ttcaactatt tggaagaagc
     4681 aaaaaatatt gccgtcgtag gcttgagcaa taaaccagaa cgaacaagtt acatggttgc
     4741 acaggtattg caagacaatg gatataaaat cataccagta aatccattat tggctggtga
     4801 agaaattcta ggcgagaaag tctatgcgaa tttgacagaa atcgatgatc caatcgatat
     4861 cgtagatgta tttagacgga gtgaattttt acctgaaata gcaggtgatt ttttgaaaac
     4921 tgatgctaaa gtgttctggg cacaattagg gattgaaaac aaagaagcag ctgagatgct
     4981 aagaaaagca ggacgtacag atatcgtaat ggatcgttgt attaagattg aacttggtaa
     5041 gttgaaagaa aaccaaaaat aaaaatgacc acaaagaaga gatgatcgat agaggggaat
     5101 gtcgaaaaag cggttaggag ataattaaaa ttgatttctc gtctaccctc ttttttttca
     5161 cgaccttttc tagtatgata atcgtggtgc ggttttgccg caaagtatta ttgaaggagc
     5221 gtttgccttg gcaaaaaaag taaacaacga atacaatgac tcgtccatcc aagtattgga
     5281 aggattagag gcagttagaa aaagaccagg gatgtacatt ggatccactg acagccgagg
     5341 cttgcatcat ttagtctatg aaatcgtcga taatgcagtt gacgaagcat tatcaggtta
     5401 tggcagtgag attgatgtga ccatccatga agacaatagt ataactgttg cagatagcgg
     5461 acgtgggatg cctgtaggaa tgcatgcttc cggaatacca acagttgaag tgatttttac
     5521 tgtgctgcat gctggcggaa agtttggtca aggagggtac aaaacttctg gtggactgca
     5581 cggtgtagga gccagtgtgg taaatgcctt atcgaaatgg ctaattgtaa cgatcgttag
     5641 agatggcgta gagtatcagc agaaatttaa aaacggcggt aaacctgacg gtactttgaa
     5701 aaagatagga aaaacaaaaa aagcaaacgg gacgactgtc cattttttac cagatgatac
     5761 aatattcagt acaacgaaat tttcatatga aattttagcc gaacggttaa gagaatctgc
     5821 ctttcttcta aaaggcgtta aaatctctct ttctgatctt cgtggagaag aaccagtcaa
     5881 ggaaattttt cattacgaag aaggaatcaa agaatttgtt gattatctga atgaagaaaa
     5941 agatacattg acaccagttg tttatttttc aggagaaaaa gaaggtatcg aagtagaggt
     6001 cgcttatcaa tacaatgacg gttattcaga aaatgtttta tcttttgtca ataacgttcg
     6061 gacaaaagat gggggaaccc atgaagctgg tatgaaagcg gcgatgacga aatcttataa
     6121 tgaatatgcg agaaaagttg gtttgctgaa agaacgggac aagaatctgg aaggtagtga
     6181 tttcagagaa gggttagctg ctgtactttc tatccgtgta cctgaaaatc ttctgcaatt
     6241 tgaagggcaa acaaaagaaa aacttggtac gccagttgct cgtaccgtag tagataacgt
     6301 catcagtgaa caaatgggct tttatttgca ggaaaatagc gaaatgagcc agatgttggt
     6361 ccgtaaagca atcaaagcca gagaagcgag agaagcagca cgtaaagcca gagaagaaag
     6421 ccgaaatggg aagaagcgta aaaaaggcga atctcttctt tcaggaaaat tgacacccgc
     6481 acaatcccga aaccctaaga aaaatgaatt gtatttagtc gaaggggatt ctgctggcgg
     6541 atctgctaaa caaggaagag acagaaagtt ccaagcaatt ctacctttac gaggaaaagt
     6601 catcaatacc gaaaaagcta aaatgcaaga tatcttgaaa aatgaagaaa tcaatacgat
     6661 gatctatacg attggtgcag gagtcggtcc tgagttttct atcgaagact gtaattacga
     6721 caaagtaatc atcatgaccg atgcggatac agacggcgcc catattcaag tattactgct
     6781 tactttcttt tatcgttata tgaaaccgct gattgaagct gggaaagtat atatcgcgtt
     6841 accgccttta tataaagtat ccaaaggaca aggaaagaag caagtcattg aatatgcgtg
     6901 gacagatgac gaattggcag caatgattaa aaaagtcggc aaaggatata tgctgcagcg
     6961 atataaagga cttggagaga tgaatgcgga acaattgtgg gaaacaacga tggatccgac
     7021 ttcccgtacg ttgatccgcg tccgtatcga tgatgctgcg caagctgaac gtcgagtaac
     7081 cacactgatg ggtgacaaag tagaacctcg aagaaaatgg atcgaaaatc acgtgcaatt
     7141 tacgctcgaa gaagatggca gtatcttaga taaaaaagag gatactgaaa tctctccttc
     7201 agtttctaat gatttattag atgaagaacg agcagataaa aatgaaaaca atcagttatt
     7261 tgaagtagaa taggaggtca cctgatggaa aatcgacaag aagtacaaga attaacttta
     7321 gaagaagtga tgggtgaccg cttcggacgt tattccaaat atattatcca agaacgtgca
     7381 ctgcctgata tccgtgatgg attgaagcca gtgcaacgcc ggatattatt tgccatgaac
     7441 aaagatggca atacgtatga caaaggattc cgtaaatcag caaaatctgt cggaaatatc
     7501 atggggaatt atcatcctca tggtgacatc agtatttatg aagcgatggt ccgcatgagc
     7561 caagactgga agttgcggga agtactgatc gaaatgcacg ggaataacgg gagtatggac
     7621 ggtgatccac ctgctgcgat gcgttatact gaagccagat tatccaagtt aagtggagag
     7681 atgctagcag atatcgagaa agaaacagtc gatctcgtgt ggaattttga tgatacagaa
     7741 aaagaaccaa cagtccttcc agctcgatac ccaaaccttc tcgtcaatgg atcgacaggt
     7801 atatctgctg gatatgcaac agaaatcccg actcataact tagctgaagt gatcgatggc
     7861 acaatctata tgatcgatca tccacaagcg agccttgaaa aattgatgga atatattcct
     7921 ggacctgact ttccaacagg agggatcttg caaggaaaag atgaaatcaa aaaagcttat
     7981 gaaacaggac gaggaaaagt tattcttcgt tcgaaaacaa agatagaggc aatgaagggt
     8041 aataaacagc aaatcgtgat cacagaaatc ccatatgaag taaataaagc gaccttggtg
     8101 aaaaaaatgg atgagatccg tttgaataag aaaatcgatg gaatagctga agtacgagat
     8161 gaaagtgata gaacaggtct gcaaattgtc gtcgaattga aaaaagacgc caatgctcaa
     8221 ggaattttga attatttgtt caaaaacact gagctacaga tcaattacaa tttcaatatg
     8281 gtcgctatcg accatatgac tccgcatcaa gttggactga aagacatttt aagaagttat
     8341 atcgagcatc gaaagcaagt cataacaaaa cgtagtcaat tcgatttagc caaagctcaa
     8401 aaaagacagc atatcgtaga aggattgatg aaagctttat cgattctgga tgaagtaatc
     8461 gcaacgattc gtgaaagtaa agacaaaaaa gacgccaaaa agaatttagt cgacgtcttt
     8521 caatttacag aagaacaagc agaagcaatt gtcacattgc aattataccg tttgacgaac
     8581 acggatatca cagagcttca aaaggaatcc gaaagcttga ttgcgcaaat cacagagttg
     8641 aataaaatcc tttcaaatga caaagagctt ttctctgtta tgaaaaaaga attgcgagaa
     8701 gtgaaaaaaa attattcttc cgctcgtcta acgacaatcg aagacgaaat cgaagaaatc
     8761 aaaattgata cgcaagtctt agtggcacaa gaagacgtaa ttgtcagtgt gacgcgagaa
     8821 ggctatgtga agcgaacaag ccttcgttct tatagtgctt ccaaaccaga agaaatcggg
     8881 atgcgagaag gcgactactt gctatactca ggtgaattga gtacattgga ccatgttctt
     8941 ctgatcacaa ataaagcaaa tgtgatctac cgccctgtcc atgaactacc cgatttgaaa
     9001 tggaaagatg ctggtgagca catttctcaa acaatctcca atctgtctgt agacgaatcg
     9061 attttagctg ttttcccata tcaaaaaatt caagcagaga aaacatttgt attcatcagc
     9121 aagaatggat tgatcaagca aacacgaatg acagagttcg aaccatggcg cacctataag
     9181 agccgtccgc tcagctgtat gaagctaaag gcggaagaag atgaactgat tgctgtgtat
     9241 ctgaaagaag atcagccagg cttagatgtt ttcttagtaa cgcatcaagg aatgggtttg
     9301 cgctatcctt taacagatgt tcccgttgtt ggagcaaagg cagctggagt gaagtctatg
     9361 aatttgaaag atggagatag gattatcaac gggctgcttg tgttagcaga aggtgatacg
     9421 ccgatcgtaa tcgttactca gcgaggagct gttaaacgca tgcttgctca agaaatcagc
     9481 caaacttcac gtgcgaagcg gggagtgacg gtcatgcgtg agctgaaaaa aaatccgcac
     9541 cgtattatct atatgtcgga aggaagatca aaagaaataa ccttgatgaa tcaaaaaggg
     9601 cagcagttaa cgattgaccc aactgatttc cctataggtg atcgaacatc gaatggatca
     9661 tttgcgatgg atgagaaaaa aggtggagaa gtcatcgaag ttatcgatgc tcctgagatt
     9721 ttcatcgagt cgtaactgtt gtagtatttt aatgaagaga cgccatctgt attaatacag
     9781 gtggcgtctc tttttcattc atttttaata aatccttata cgtttgcaaa aaaagatgaa
     9841 agtgttcaca atgcttaaat gttggtatgt caacgttgat ttaaatatat gtaaataaaa
     9901 cataaaaatt gttaaataaa aaacaagaaa acccttgcaa agatagggtg ctgtgttata
     9961 ctaatcacgt aaaaactgtt acataaaaca gaaatttagg aggtccatac aatggagcaa
    10021 tggaatggat ttaaaggtac caaatggaaa gaaggcgtag acactagaga ctttattcaa
    10081 aataactaca ctgaatatcg cggtgatgac agctttttgg aaggcccaac agaagcaaca
    10141 gaaaaattat gggatgaatt gcaaaagtta aatgatatcc aattcgagcg taacggtgtc
    10201 tatgatatgg acacagaaat cgtatcaact attacttcac acgatgcagg ctatttggat
    10261 aaagatttgg aaaaaatcgt cggtctacag acagacaaac ctttgaaaca agcattcatg
    10321 ccgttcggcg gaatcaatat ggctaacaat gcgctaaaaa gcaatggcta tacaccaaac
    10381 gatgaattga cacatatttt cactgactgg cgcaaaacac ataatcaagg tgtatttgac
    10441 gcttatactg ctgaaatgcg tgctgctcgt aaaaacaaaa tcattacagg gcttccagac
    10501 gcttacggtc gcggacgtat catcggtgac tatcgtcgtg tggcattgta tggaatcgac
    10561 tatttgatgg aacaaaagaa aaaagatcat gatatgactg gttatcatac aatgagtgaa
    10621 gaagtgattc gtttacgtga agaaatcact gaacaatatc gtgcattagc gcaaatgaaa
    10681 gaaatggctg cgaaatatgg ctatgatatt tctaaaccag ctgcaaatgc aaaagaagct
    10741 gtccaatggc tatacttcgg atatttagca gcaatcaaat cacaaaatgg tgctgcgatg
    10801 tcaatcggac gtatctcagc attccttgat atctatatcc aacgtgattt ggataatgga
    10861 gtaatcacag aaaaagaagc acaagaattg atcgaccact tagtaatgaa attacgtatg
    10921 gtcaaattcg cgcgtacacc tgaatataac caattattct caggattccc tatctgggct
    10981 actttatcca ttgcaggtat gggattagat ggtcgttcat tagttacaaa gaatgatttc
    11041 cgtctgcttc atacattaac aaatatggga ccttcaccag aaccaaactt gactgttttg
    11101 tattctgaac accttccaga aggattcaga acatatgcta gcagaatcgc tatcgaaagt
    11161 tcttcgattc aattcgaaaa tgataatcag cttcgtgaac aatggggttc tgatgattgt
    11221 gcgattgctt gctgtgtatc aggaacagtt gtcggaaaag acatgcaatt ctttggcgct
    11281 cgtgctaact tggctaaaac actattatat gccatcaatg gcggagtcga tgaagtgact
    11341 ggcgcccaag ttggaccaga atatcgtcca atcacatcag aaatactaga ctttgaagaa
    11401 gtgaaacata acttcatgaa tatgatggat tggttagcag aactatatgt caatacgttg
    11461 aacatcattc attatatgca tgataaatat tcttatgaag cagctcagct ggctttgatg
    11521 gattctgact tgcaacgcac atttgctaca ggtattgctg ggatctctca tgcggcagac
    11581 agcttgtcag cgatcaaata tgcaaaagta aaagcaatcc gtaatgaaaa aggaatagca
    11641 gttgattttg aagtggatgg cgacttccct aaatacggaa atgatgatga ccgtgcagat
    11701 gaattagttt catggatctt ggaatacttc atgggacaaa tcaaacgtca taaaacttac
    11761 cgcaactcaa aaccaacaac ttcattattg acgattactt caaatgttgt ttacggtaaa
    11821 aatacaggaa atactcctga tggccgtgaa gctggaaaac cacttgcgcc aggagcaaat
    11881 ccaagttaca atgcggaaca aagtgggtta ttagctagcc tgaactcaac agctaaaatg
    11941 aattacggct gtgcattgga tggaatttca aatacccaaa caatcaatcc ttcaggtctt
    12001 ggaaaagaac aagatacaag aatcgaaaac ttgcgtaatg tcttagatgg atacttctca
    12061 aaaggagctt atcatttgaa tgtcaatgta ttcagtaatg atttattgcg tgatgcaatg
    12121 gaaaatccaa tgaaatatcc gaacttgacg atccgggtat ctggttatgc agttaaattc
    12181 cgtgacttga caagagaaca acaattagat gtcttgatga gaacttcaca cgacagaatg
    12241 taaaaagttc gtgaaaaaag tttttcgact tagcgtcgaa aggacgttga atagccatcg
    12301 catcaaaatc gaataatcag tatgcttatt tgaaagaaac gatttacagg ggcaggactt
    12361 gtatgttccg tccctgtaaa ttttacacat aacgaagcgg caattgcgaa aaattttgtt
    12421 cagaaagctt cgcttcgaca gctaacttgt acttaaaatg aaatcaaaga aaagatgtga
    12481 acttaaatgg aagaaaaaac aatcggctat gttcattcta tcgaaacttt cggttcagta
    12541 gacggacctg gactgcgctt tgtcgtgttt atgcaaggat gccgcatgcg ttgccaattt
    12601 tgtcacaatc cagatacttg gaatattggc ggagggaaag aatacacagc agatgaactt
    12661 ttagataaag cagaacgctt cagaccttac tggggagata aaggtggtat tactgttagt
    12721 ggtggagaac ctctattgca gatcgatttt ttgatcgaac tatttaaaaa agcaaaagag
    12781 cggaagatgc atactacatt ggatacatgc ggaaaacctt ttacgtatga agaacctttc
    12841 ttttcacgtt ttcaagaatt gatgaagtat acagatttac tgttatttga catcaaacac
    12901 atcgataatg aagagcataa aaaactaacg cattgggaca atgaaaatat tttagaaatg
    12961 gctcaatatt tgtcaaaaat caacaaaccg gtttggattc gccatgtact agttcctgag
    13021 cgaagtgatt atgatgaata tttgatccga ctagacaact ttattcaaac cttgtcaaat
    13081 gtagacagag tagagattct tccttatcat acgatgggaa aatacaagtg ggaaacactt
    13141 ggtttgaaat atccattaga agggatcgaa ccacctacaa aagaacgtgt cgaaaatgct
    13201 aaacgattgc ttcatacaga agagtataca aaatatttaa cgcgataggt cgcatcggaa
    13261 agaagataag tattgcttct ttccgttttt ttgctattat taaataggag aaatcacaaa
    13321 ggagcgaatg acatgtcaaa aattttagtt tttggtcacc aaaatccaga tacagatgca
    13381 atcggggcag ccattgcgtt ttcacacttg caaaatgaat taggtaaaga aacagaagca
    13441 gttgctctag gtacacctaa tgaagaaaca caatacgcat tagaccactt tcagctatct
    13501 gcaccacgag tagtagagtc agctaaagca gaaaatgcag atgtcatgct tgtggatcat
    13561 aatgaattcc aacaaagtat ttcagatatc agcgaagtaa atattttagc tgtagtagac
    13621 caccatcgta ttgcaaactt tgaaacagct aatccattgt actatcgtgc agaaccagtt
    13681 ggatgtacaa gtacgattat tttaaaacta tataaagaaa accaagtaac aattcctaaa
    13741 gaaatcgcag gaatcatgct ctcagctatc atttcagata cattgttgtt caaatcacca
    13801 acatgtacgc ctgaagatga agcagctgca aaagaacttg cagcaatcgc tgaagtcgat
    13861 ctaaacactt atggacttga aatgttgaaa gcaggaacaa atctaagtga taaatcagct
    13921 gcggttcttc ttgatttgga tgcaaaaagt ttcccaatgg gcagttccaa cgttcgtatc
    13981 gctcaagtca atacagtgga ccttaatgaa gtgatggatc gacaagcaga gctagaagca
    14041 gcaatggaag atgaaatgaa agcaaatggc tacgatctgt tcgtattagt tgtcacaaac
    14101 atcttagaca gtgattcaga attacttgtt gctggtaaag gaaaagaaaa agtagaagaa
    14161 gctttccaaa caactttgac caacaaccgt gctttgctta aaggtgtcgt ttcacgtaaa
    14221 aaacaagtcg tacctcaatt gacggctgta tttagctaat caaatggtag tatgaagcat
    14281 tcaaaatgaa tggataagcg attgatataa aaagaaacat agaacatact aaaggccgaa
    14341 gaaatggagg agaataacag tcaattatga ctcttgttct tcctcctttt tacgtaaaag
    14401 ccttttaagt aaaataaaac ggatagaaaa gagaacatat atgaactatt attcaaaaaa
    14461 taaggaaaac ttttatcaaa aactgaccga tgatccgctt ttttcttcat tgaccgatta
    14521 tctatatgaa catcgagaac aagaaacgat tctaagagaa ttgaaaaaag aattttcgca
    14581 aaataaattt tcccattttc ttgatttatt gatcgatgca ggattaatca agagggaaga
    14641 acgaagatat catttgaact ttccaatatt tgatcctaaa gattacttgc aacaagccac
    14701 ttcggctgct gaaaccatag ctgaacaatt gaagagactc tctgttgatg aacaaaaatt
    14761 agcgatggga gaagttattt gggcgtattg tttcgaagat gaacggaaag aagcgtattt
    14821 ttatggagta agaaacagcc gagagacaga attgctgagg acaactgcag gaaatgagaa
    14881 gtaccgtttt atcacacttt cctctataga acacttcccg cttactttgg ctaattactt
    14941 ttttgtacag aagaatcagc ttcctgtaac gaaggcattc aaagaattag ccgaacttat
    15001 tggagacgta aatgaatcgt acttttttga tcaaatagaa gttatcgttg acaggatacg
    15061 taagaataaa tataaaaatc gccggcctag tattttccat cagtctctac ttgtgacgaa
    15121 tactataaag gaagaagaat cattcacatt agaattacct attgtcgaaa aaaacaattt
    15181 tgagattgaa ttaccaacat tggatccttc attgacaatg gaggaaatgg cctttcttaa
    15241 acgacaaatc ttttctgagc tgagcaagaa atttataccg catgcatttt cttatatcaa
    15301 agaatatgga acagttttag tgagtaaaac ctaagagagc agtaagcaaa aattatactg
    15361 actcttgcag gaggagcctt tctcttcctt tatggttaaa agaattattt gagtattttg
    15421 cctatctacc acctattaga ttcactacat ggtctggaaa caaaggaatg caaaaaaatg
    15481 gtgagaaatg tggaatgaca aaggaagtag tgataagaaa agttagattt ttaaataatc
    15541 aatattatga ttcagtcaag tatggcattt tatgggaaga actagctgat tagattagga
    15601 agtccatttg ataatccaga atcactaaga aacgaatggc gagtcattaa taatttatcc
    15661 aatcttgggt tattctttta gattgaagtg tttaaaacta aaaaagcatt tatgaagtcg
    15721 taatagtcag gcttcaaaat gctttttatt ttgtttgttt tttctttgcc acataaatac
    15781 acctagactg acgatgaaga gcatatccaa cagatttcct gcgacttgga tcgtcgaagg
    15841 gttggtcagt ggcattaaag caaggacaaa aaaggaaaac aagctgtaaa taaaacaggt
    15901 gacaggaact aatcgaaaga agagcggaca tttcaattgg ctaaaaatga gtgaaaaaat
    15961 aaaataagta aaaatggata aaatcacgac taaaccattc atgataaaga agaaaagcgt
    16021 attgtgatta aacgaataaa aaagaattgc taaaaaagaa aacgttttaa atgcaatcaa
    16081 tacgcttgaa gcttcgttta acgtaccagg atgtcgatga aaaaaatagg caccgaaata
    16141 aacagaaaac agtaagccgc aaagagaaga caaaatcaaa aaaaacggat tttcttcggc
    16201 ttctgtgata tcagaatgat ccaaaagcaa ccagcctaat gcactagaag gcgcatgtga
    16261 ggtcttaagg tatgaataac atagaaaaaa taagatcgtc aaagttgcaa aaaaagagcc
    16321 taaatgtaaa aaattagtgg ttctacgttg aatttccata at
//
LOCUS       DAYYCY010000050        16355 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000050 DAYYCY010000000
VERSION     DAYYCY010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.050"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..258)
                     /locus_tag="ACHAZB_001289"
     CDS             complement(<1..258)
                     /locus_tag="ACHAZB_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8613975.1"
                     /translation="MERQESIREVQEVPTEQAKETQEGRSFSYGKNFSFLRPSIQISV
                     FVLWRIHNNRYSVGSQLFYQEICHALSKKYSQNAYKEALAFL"
     gene            complement(274..924)
                     /locus_tag="ACHAZB_001290"
     CDS             complement(274..924)
                     /locus_tag="ACHAZB_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8613976.1"
                     /translation="MKKRFIYVCVGGLFLFGVGSTFFLIHEKLQLTAKEEKLTVEINK
                     FQEEKKGQEDLVTSLKEQLQQVESEVTTLKKANEELKNNDSTEKGNIQTFQKTVTEAF
                     EVLFNYEPDSFQERKNNCAPYLSDKLMKQYFKEGVSYGDSNGVSSELEKCSVYLKSTQ
                     KATIQGLVVVSYRNKIGDSSWNNAMNIFDVTFDPASKKITFMQNLGSSFNQQLLEE"
     gene            complement(935..2107)
                     /locus_tag="ACHAZB_001291"
     CDS             complement(935..2107)
                     /locus_tag="ACHAZB_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tip lysozyme"
                     /protein_id="HFM8613977.1"
                     /translation="MKKKLLLWCGFGCLPTLLLCMFGFLFMILLISSGSEEEEIDCSP
                     TEIVASTISLTNQGMETNGLQIAKEVQKHIPNTTLEGLAGMLGNFQQESSMSPTAIER
                     PNDPLSGHGIAQWTAGRTTALKNFAKERNKEWSDLGVQVEFLIHELNTTEKASQVALQ
                     AKSVEEATEQWQVKFERAGIPMMGNRITFANQWYAKLGKNDSDPVASITLENGANAGS
                     KDLTDYCLETTGTSSSSDSNVLNVAKSLIGYFHYSQPQRAQFGSVENPDKNGYADCSS
                     FVWLVLTKAGYKTPANVGWFTGSMTSDARGSKQYLQEIPESEAKAGDIIIVNLGSGVG
                     NDGHTAVLAEDWHGLSTKVIEQGGMNTNGVGEGQVNLSFGYLLNGGDICFARPIKK"
     gene            complement(2126..2998)
                     /locus_tag="ACHAZB_001292"
     CDS             complement(2126..2998)
                     /locus_tag="ACHAZB_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFM8613978.1"
                     /translation="MKKMILGSLFSATVLLGMVGTSAYADTTSPGDPSVPPTEIIPPT
                     EETPEVPEEPVLPPTDGGEETPEIPEIPETPETPEIPPTEENPDTGGGTETPEVPENP
                     DTGGGEGTPEVPPVEENPGNGGNTETPEKPSNPGGNEGNTTPPTTPPTNNGGTNTTPP
                     VQDVVVKPDGSIGSQQTGGQNVQIETNNASELTHIPTPSTPIETASGEKIVSVVDGVA
                     YKESGNGLTPISDKVEQLPSGNIAVKGSDGKMKVLPKTGEEVSLVATALGGLLASGSG
                     FAFWRKRKATQKMN"
     gene            complement(3020..3940)
                     /locus_tag="ACHAZB_001293"
     CDS             complement(3020..3940)
                     /locus_tag="ACHAZB_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8613979.1"
                     /translation="MMMIRFTEVDNMSLLTDKKIALDKAFIQLKRENEELLARAEIGW
                     CTLEIYTENHEIPVFEGSYDFPVEEFHLISLIEQSLTENGQLSEEAQTFIQTLNSELP
                     KFYQVKKDQKNERRPTKIKKEKKTPLSVETAFTQSNGSLQKKKMSFQWLLFGFIVVVS
                     CGLFIMQLSTQREIEQLTKKEHQLQERIAFAEELATKQPQIDTFSRYFITNYYSGEMA
                     EERYRKKLQSFLTEKVAKQISPQKEKVKSILSWEMKKEGKHWQIAYVITLETEKKTQE
                     TKKVSFQLEEQEKNYLVSTLPELDDFEINQ"
     gene            complement(3940..4209)
                     /locus_tag="ACHAZB_001294"
     CDS             complement(3940..4209)
                     /locus_tag="ACHAZB_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8613980.1"
                     /translation="MIIVTGIVLLGIGVLFGYHFAYFPKIGEWQQRQAIQKYFQVKGN
                     EYTYYAEDEDSYIVSLNNQEYRVKFSLNNPIQVVYAVEVVEEVFD"
     gene            complement(4206..4586)
                     /locus_tag="ACHAZB_001295"
     CDS             complement(4206..4586)
                     /locus_tag="ACHAZB_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFM8613981.1"
                     /translation="MITNYKKFWQNSFNYKGKATRKEYWLVALMNALIGFGLGLAIGL
                     AGALNASETIIGCLIGVLIVYCFVVLVPSLSLSVRRLRDAGFHWAFIFFYFVPTIGSL
                     VLLVLYCLPTKESIEDKQVQEETR"
     gene            complement(5053..6516)
                     /locus_tag="ACHAZB_001296"
     CDS             complement(5053..6516)
                     /locus_tag="ACHAZB_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFM8613982.1"
                     /translation="MKKTSKCLSALLLVGQVFGYTPAVLANEQGNNQLTNSQSATITE
                     ETTTNSTGEENQPETPTEDTIDTPDNPEMNAPPTGSEEQPETPNQPDKPEDNQQPEEE
                     PSEEPNEQPSDESSQTPQKPSTETEAEPPTKTERPQKAPEQSVTVPMNDVPQAQSIGS
                     EETKNYHQAPSAPIQELVPSSTQDNEGTIHFEKDASVETFIRKIGESARKIGQENDLY
                     ASVMIAQAILESASGQSQLAQAPNYNLFGIKGTHNGKGVSFATQEDLGNGTLYTTQAT
                     FRQYESYEESLKDYAKLLKEGLVGNTSFYDGVWKSKAKTYQEATKFLTGRYATDTSYD
                     KKLNGLIETYDLIKYDKEVAGPQLSKEGYMVPLKNYSISSSFGWRGEEFHRGLDMAAN
                     QGEPIYASKAGTVIEASYHTLSGNYVTIEHEDGSVALYAHQQEYCVNVGQTVEQGQII
                     GYVGSTGNSTGPHLHLELALDGSLTKDKLINPETVLF"
     gene            complement(6580..9597)
                     /locus_tag="ACHAZB_001297"
     CDS             complement(6580..9597)
                     /locus_tag="ACHAZB_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFM8613983.1"
                     /translation="MFKRMATWLAIFVTFLNPMLGSVTAFAETINESNSTEEVMKTEE
                     SKSSEEAISTETTSETTEETTTQDTTEDVSPPNVMTQEVPEGYEKYFITIEDIQKATS
                     FSEQERLAQESEEWQAILEKNAQTRATNLTLTENGRKDVNMTRITPEGTMYTHDYMVR
                     WQIDGEDVFCIQEGAITSAGIQYDVTPLENLITTSAIRKKISLIGYFGYYSSGSMDNY
                     VLAQTYIWEVLGGSFVNYGTIGQSNYEAFKQTVDRKINNLLEDPSFDSQTVTVKVGEE
                     VEVKDTKGVFSQWDGSVVENTANVQVRKSGNSLFIKANQNSNDGRISVSSLDPNSRGV
                     GSSFAYVDPVAQNLGKLYVPDPNRAGLTIKVLKNGDFKLTKLDAVTGKKVPNVTYEIK
                     RHDTGETKQYTTDAKGEIALKEIPAFYEKDGKYVQNKLDVKEVKAPAGYILDKQTHTI
                     TIEPNKEVTLTAKNDLQQFIPEGKKVKEVYNAEESWNQGKPVYDTVPAENIEFDIIAK
                     TDITAPDKETVLVKKGEVLDSVKTDKDGHWKANKKFYNGDQNKYQLIEKNVPDNYRQP
                     SEEQTTFSVAYGDNTTPVLTFELDVIDNLLKTGEVDFNKVDSINHLQLPGAKFLIEGM
                     TAHNKNVSFVYETKEEPEKLKLPDGTYKFTEIVFPEHYGQASGQTETILVTVKDGEVQ
                     PITVKNEEIVVPEEDQPEISTLFVTTTGGKDFNPKKDQELVDKVSQVFPKSAVGTEKY
                     WVTQFHKITADGKVTVLDTVKETRKVTDEKETFDVTFNYKADMVSDGDTIVATHIVYN
                     DKEEEEEFARHFDLTNKDQTVTAKEPKIETRFITVNGDQTFDPDKDQELIDHVSQEFP
                     ESTVGETKYWVTQFHKIDKNGKDTVVGTVETARKVSKASEEFKVSFDYKAGTLKDGEK
                     LVATHIVYKDKDHKEEEARHFDLKNEKQTLTAKGIKKETPTTPVKQTPTSTPKQSLPK
                     TGETVNDVLIALGLFLFVGVGAVLVRRKMKQSN"
     gene            complement(9748..10587)
                     /locus_tag="ACHAZB_001298"
     CDS             complement(9748..10587)
                     /locus_tag="ACHAZB_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HFM8613984.1"
                     /translation="MKMLKVLALGIVVGGVSLAVGTNAEAAENKVWTPRSAEQIKAEI
                     KGNEYTIVWGDTLSAISQATNITVQKLADMNKITNVDLIYAGNKLVFNGNVASVQNSA
                     GETVAQTVIQEKDKVDPNQPVGQGTTSNTQTTPNTTTPTTPAGDQTGTTGGTTTPSTP
                     ENNGGTTQTPTQPGNGGNTGGETTPSNPSTGGGTTTPEEPSNPGGGDQGGNGGTTTPE
                     EPTIVVGEIGNSGKVFNTVDEAYDWANAEVSDPNSQWYNKGFMVDVIIYSDGSQKHTV
                     DFY"
     gene            10732..10977
                     /locus_tag="ACHAZB_001299"
     CDS             10732..10977
                     /locus_tag="ACHAZB_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8613985.1"
                     /translation="MQLNNLNEIHDWAQNNLLNRKQAAKITGQNYDAFSSSLRRNNIK
                     PFLEIGDEPGPSIIRLYLREDIEKYAKQLEEKRQKRQ"
     gene            11040..11462
                     /locus_tag="ACHAZB_001300"
     CDS             11040..11462
                     /locus_tag="ACHAZB_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8613986.1"
                     /translation="MNKKYIVSVGSIVLIVLAIIAFSVSNKTETQPQNTVQSTTTSQS
                     SDHSVYPSLNSLKETHGQDLIDLGSSQITTVLFEKKNIAFITPKEGTDLEFSIKVTNT
                     KENLFSIPDIQYTPNKLKIGDTFGLAKVNEQYFAYSLK"
     gene            complement(11556..11873)
                     /locus_tag="ACHAZB_001301"
     CDS             complement(11556..11873)
                     /locus_tag="ACHAZB_001301"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8613987.1"
                     /translation="MPVKGTSNFDFEIFYVDDEICGVFNKQKYTKEQAIEAWKNELFD
                     PEEEISFVVDESFVGWYHGNAEEEEVHYAGWWLEEGEPREQSVPVWVISENPLEEIEK
                     LEK"
     gene            complement(11900..12106)
                     /locus_tag="ACHAZB_001302"
     CDS             complement(11900..12106)
                     /locus_tag="ACHAZB_001302"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8613988.1"
                     /translation="MNWLKNEAELYVFHEEMQEKHLVPNVRNYLFWLEEKNDAQVFGK
                     SAQSPKIKLQLTSKQWNEHEWYIY"
     gene            complement(12087..12269)
                     /locus_tag="ACHAZB_001303"
     CDS             complement(12087..12269)
                     /locus_tag="ACHAZB_001303"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8613989.1"
                     /translation="MTKCERVSVTISFIALLLSVISLLNTAIFIGQVEKQKEISLSEW
                     RYEEVNEGMNNELVEK"
     gene            complement(12283..12633)
                     /locus_tag="ACHAZB_001304"
     CDS             complement(12283..12633)
                     /locus_tag="ACHAZB_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8613990.1"
                     /translation="MKKSYKVFENNEIKEQEAIILSAQTPEEALEQALPFLVESQNIV
                     VEELPEFDSKIVFSFNCKKESEISVSPEFYCRSDISVEGLVMCASAFIEIFANHLQIS
                     VKELLDLLQEITNE"
     gene            complement(12659..12961)
                     /locus_tag="ACHAZB_001305"
     CDS             complement(12659..12961)
                     /locus_tag="ACHAZB_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8613991.1"
                     /translation="MLSKQEKKYIRQKMYAMIGNRAITELSSEELLQVQQLAQIIGSN
                     SIFDSNPLPEMTLETLTAKRYKELRAIGYRMIDIRRALRISDTKFREWRTSNGLAN"
     gene            complement(12964..13197)
                     /locus_tag="ACHAZB_001306"
     CDS             complement(12964..13197)
                     /locus_tag="ACHAZB_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8613992.1"
                     /translation="MTISVGNKVKYIGTAIPRYTNKLVVVKNILVNGLILEYPKEDRR
                     KVVVEGNGTWNMNSFICGFTEVEVTNDNENRSG"
     gene            complement(14101..14631)
                     /locus_tag="ACHAZB_001307"
     CDS             complement(14101..14631)
                     /locus_tag="ACHAZB_001307"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8613993.1"
                     /translation="MKMTPNHLRHICCEVTLFNRQRWKLSNRIVRRIIVDSTGKSTVI
                     LTKKLATKQDFQLFFSQVKTIFFDGKIIANRGCSNQDLMVSNIVFSRMKRRQDLLIQA
                     RKAYKVFKKQEKQSRGKQNEQIMNKKCIEVLNGKNDHSDTKKKEGRHENYPILLVCPN
                     CFSCGFVGRIGFRTWN"
     gene            complement(14628..15743)
                     /locus_tag="ACHAZB_001308"
     CDS             complement(14628..15743)
                     /locus_tag="ACHAZB_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFM8613994.1"
                     /translation="MATKRFTATRKYNELYYQFPKVFITSEKYRSLSDKAKIAYMVFR
                     SRLDLAIQRNQVDSQGFVYFEFTEQELANALNCCRRSVNTIKKELKEYELLEIEDMGF
                     DQQTGKRNKARLYLGELDTTENDVYSLGPVDSTSVDSAQGKEEEESVDNLAIFDDFDE
                     KNPCENSAQLFNNSSNNLDTENLDTKIDTTSPVEMADDWQKEFIAHYSESTFVPKDVL
                     TWIVRSSAEWREAKEQLNMIYDSKRIIEKRQRKYNQTHGLSDGSEYRILGETFAEDLS
                     KTYQRCIQNEKIARHEGKAIQNRKGFYFRALLNFWECCVRELNLDPMATKLSDQAEYS
                     LLQAKRSCSYEDLETQIYGLSQLPKISMHNWLEELDT"
     gene            complement(15749..16147)
                     /locus_tag="ACHAZB_001309"
     CDS             complement(15749..16147)
                     /locus_tag="ACHAZB_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFM8613995.1"
                     /translation="MVKNKFRATDVLTQATFHMPKLFFIKSSKYYAMTAQAKYAYSLL
                     NEYLEESLIEKRIDEKKHVYISYTNQELAILLNVSEEEIEEIKNELEDYDLLTIEFGR
                     IYLKELIATMEDVRVYQHQNSFLIYGQDEV"
BASE COUNT         4331 a         3421 c         2564 g         6039 t
ORIGIN      
        1 caaaaaggca agtgcttctt tgtaggcatt ttgactgtat ttttttgata aagcatgaca
       61 aatttcttga taaaatagtt gactgcctac cgaataccga ttattgtgta tccgccacaa
      121 aacgaacaca ctgatttgaa tcgacgggcg taaaaaagaa aaattctttc cataagaaaa
      181 agaacgtcct tcttgggttt cttttgcttg ttctgttggt acttcttgta cctctcttat
      241 tgattcttgt ctctccataa ccttctcctt ttcttattct tctaaaagct gttgattaaa
      301 gctacttccc aaattttgca taaacgtgat tttctttgac gctggatcaa aggttacgtc
      361 aaaaatattc atggcattat tccaggaact atcaccaatt ttattgcgat acgacaccac
      421 aacgagccct tggatagtcg ctttttgtgt gctttttaaa taaacagaac atttctctaa
      481 ttcagaagac accccgtttg aatcaccata ggaaacgcct tctttgaaat attgcttcat
      541 cagtttgtca gaaagatatg gtgcacaatt gttttttcgt tcttgaaaag aatcaggctc
      601 ataattaaac agaacctcga acgcttctgt cactgttttt tgaaaggttt gaatgtttcc
      661 tttttctgtt gaatcattgt ttttcaattc ttcatttgct ttttttaacg tcgtgacctc
      721 gctttctact tgttgaagtt gttctttgag tgacgttaca aggtcttcct gtcctttctt
      781 ctcttcttgg aacttgttta tctccaccgt cagtttctct tcctttgctg ttagctgtag
      841 cttttcatga ataagaaaaa atgtactgcc tacaccaaat aagaacaatc cgcctacaca
      901 aacataaata aatcgtttct tcatttgaca cctcttattt ctttatcggt cgagcaaaac
      961 aaatatcacc accattaagc aaatacccaa aggataaatt gacttgccct tcacctacac
     1021 cgttagtatt cattccccct tgttcgatta ctttcgtaga aagtccatgc caatcttccg
     1081 ctaaaacagc tgtgtgtcca tcatttccca caccagaacc taagttcaca atgataatat
     1141 cgccagcttt tgcctcgctc tcaggtattt cttgtaagta ttgcttactt cctcttgcgt
     1201 cgctcgtcat tgagccggta aaccacccaa cgttcgcagg cgttttatac cctgctttcg
     1261 ttaaaacaag ccacacaaag ctcgaacaat cggcatagcc atttttatct ggattttcga
     1321 cactaccaaa ttgtgcccgt tgcggttgtg aataatgaaa ataaccaatc agactttttg
     1381 ctacgtttaa tacattacta tcggaagaac tgcttgttcc tgttgtttct agacaataat
     1441 cggtcaagtc tttacttcct gcgtttgccc cattttctaa tgtaattgaa gcgacgggat
     1501 cactgtcatt ctttcctagt tttgcgtacc attgattcgc aaaagtaata cgattcccca
     1561 tcattggtat ccctgcacgc tcaaatttca cttgccactg ttctgttgct tcctctacgc
     1621 tttttgcttg taatgcaact tgtgaagctt tttctgttgt gtttagttca tgaatcaaaa
     1681 actcaacttg tacacctaaa tctgaccact ctttgtttcg ctctttggca aaatttttca
     1741 aagcagtcgt tcgccctgcc gtccattgtg caatcccatg cccagataat ggatcgtttg
     1801 gtcgttcaat cgctgtggga ctcatactcg actcttgttg aaaattgcct aacatacccg
     1861 ctagaccctc taatgtcgta tttggaatat gtttttgtac ttcctttgca atttgaagcc
     1921 cgtttgtctc catgccttgg ttcgttaatg agatagtact tgcgacaatt tcggttggac
     1981 tacagtcgat ttcttcctct tcagaaccac tactaatcaa aagtatcata aacagaaaac
     2041 caaacataca tagtaatagt gtgggtagac aaccgaaccc acaccataaa agaagttttt
     2101 tcttcatgtt tgatccttca ttcgcttagt tcattttttg tgttgctttt cttttgcgcc
     2161 aaaaagcaaa accgcttcct gaagccaata atccaccaag tgctgtcgca acgagagaca
     2221 cttcttcgcc agtcttaggc aatactttca tttttccatc agaccctttc accgcaatat
     2281 ttccgcttgg aagttgttcg accttgtctg aaatgggcgt caagccattt cctgattctt
     2341 tataagcgac accatcaacg acggagacaa ttttttctcc tgaagctgtt tcaattggtg
     2401 tacttggtgt tggaatatgg gttaattcag acgcattatt ggtttcaatt tgaacatttt
     2461 gtccacctgt ttgttggctg ccaatacttc catctggttt tactacgacg tcctgtactg
     2521 gaggcgtagt gttcgtcccc ccattattgg taggtggtgt cgttggagga gtcgtatttc
     2581 cttcattgcc tcctggattc gacggctttt ctggtgtctc tgtattacca ccatttcctg
     2641 ggttttcctc aacgggtgga acttcaggtg ttccttcccc tccgccagta tctggatttt
     2701 ctggtacttc aggcgtttct gtgcctccac ctgtatctgg attttcttcc gttggtggaa
     2761 tctctggggt ttcaggagtt tctggtattt cgggaatttc tggggtttct tctcctccat
     2821 ctgttggtgg taaaacaggt tcttcaggaa cttcaggtgt ttcttccgtt ggtggaataa
     2881 tttctgtcgg tggtacactt ggatcacctg gtgacgtagt atctgcatac gcactggttc
     2941 ctaccatacc tagcaataca gttgcactaa ataaacttcc taaaatcatt tttttcatgt
     3001 gtttttcctc ttctcttatt tattgattta tttcaaaatc atctagttcg ggaagcgtgg
     3061 aaactagata atttttttcc tgttcttcca attgaaaaga cacttttttt gtttcttgtg
     3121 tctttttctc ggtttccaat gtaatgacat acgcaatttg ccaatgcttg ccttctttct
     3181 tcatttccca ggataaaata cttttgactt tttccttttg aggactgatt tgtttcgcaa
     3241 ctttttccgt tagaaacgat tgaagctttt tcctataccg ttcttctgcc atttcacccg
     3301 aataataatt cgtaataaaa taacgactaa atgtatcaat ttgtggttgt tttgtcgcaa
     3361 gttcttccgc aaatgcaatg cgttcttgta actggtgctc ctttttcgtt agttgttcaa
     3421 tctctctttg cgttgaaagt tgcataataa aaagaccaca agatacaacg acgataaatc
     3481 caaacaaaag ccattgaaaa gacatttttt tcttctgaag agagccgttg ctttgtgtaa
     3541 acgctgtttc tacagaaagt ggtgttttct tttccttttt gatttttgtt ggtcgacgtt
     3601 cgttcttttg gtctttcttt acttgataaa acttcggtaa ttcggaattt aaggtttgaa
     3661 taaaggtttg tgcttcttca cttaattgcc cattttctgt taaagattgt tcaatcaatg
     3721 aaatcaaatg aaactcttct actggaaaat catacgagcc ttcaaatact ggaatttcat
     3781 ggttttctgt gtagatttcc aaggtacacc agccaatttc cgctcgtgcc aatagctctt
     3841 cattttctct tttcaattga ataaatgcct tatctaatgc aattttttta tctgttagta
     3901 aagacatgtt atcaacttct gtaaatcgaa tcatcatcat tagtcaaaca cttcttccac
     3961 cacttctacc gcatacacca cttgaatagg gttatttaaa gaaaatttca cccgatattc
     4021 ttgattgttc aaactgacga tatagctatc ctcatcttca gcataatagg tatattcgtt
     4081 gcctttgact tgaaaatatt tttggatcgc ttgtctttgt tgccattcgc caatctttgg
     4141 aaaataggcg aaatgatagc caaataaaac gcctatccct aataaaacaa ttcctgttac
     4201 tataatcatc tggtttcctc ctgtacctgt ttatcttcta ttgactcttt tgtcggtaag
     4261 caataaagaa ccagtaacac aagagagcca atcgttggga caaaatagaa aaaaataaat
     4321 gcccaatgaa aacctgcgtc tcgtaaacgt cgaacgctga gagaaagaga cgggacaaga
     4381 actacgaaac aatacacaat caatacgcct atcaaacaac caattattgt ttcagaagca
     4441 ttcaacgctc ctgctagtcc aatcgctaag cctaggccaa acccaatgag ggcattcatt
     4501 aacgcaacga gccaatactc ttttcttgtg gctttccctt tgtaattaaa gctattctgc
     4561 caaaattttt tataatttgt gatcatatct ttgtcctttc taatgtttat ttttgattct
     4621 agtttcttct gttttactaa caatgaaact tgccataaaa aaggtggcca atacaccacc
     4681 agctagaaat ccacttacaa ataacaaaat tttttcctcc tttctaaaaa atttcgccat
     4741 aaaaaggcaa agtatgaaca cgataaatac catgtacata ctttgccttt ttatgcaata
     4801 tatgttaact ctccaagcaa ttactgattt ttaaattctt tataaataaa ataaaaatcg
     4861 tttgagtaaa gaaataagtc aatgaagacc caaacgataa aaaatattcc taatactata
     4921 tttaataaac tacgttctag atagatacga gatttcaaat taaaaaacga aaatgagaga
     4981 tacaaatata tcaaatattt aaaaagtaac cacattttct ataccactcc ttatcagaaa
     5041 cagccttatt ctttagaata agactgtttc tgggttgatt aatttgtctt ttgttaaact
     5101 accatctaag gcaagttcta aatgtaaatg tggtcctgta ctatttcctg ttgaacccac
     5161 ataaccaata atttgtcctt gttcaactgt ttgtcctacg ttcacgcaat attcctgttg
     5221 gtgcgcataa agagccacac ttccgtcttc atgctcaatt gtgacataat ttcctgacaa
     5281 agtatgatac gaagcttcta tgaccgttcc tgccttgctc gcatagattg gttctccttg
     5341 attggctgcc atgtctaatc ctctatgaaa ttcttctcct cgccaaccga aggaactaga
     5401 aattgaataa ttttttagag gtaccatata gccttctttg cttagttgtg gaccagctac
     5461 ttctttatca tatttgatta agtcataggt ttcgattaac ccatttaatt ttttatcgta
     5521 gctagtgtct gttgcgtatc ttcctgttaa aaatttagtc gcttcctgat aggtttttgc
     5581 cttacttttc caaacaccat cataaaaaga ggtatttcca actaagcctt cttttaacag
     5641 tttggcatag tcttttaagc tttcttcata gctctcatat tgccgaaaag ttgcttgggt
     5701 cgtataaagt gtcccattgc ctaaatcttc ttgtgtcgca aaagagacgc cctttccgtt
     5761 atgtgtgcct ttgattccaa atagattgta gtttggtgcc tgagccaatt gactttgtcc
     5821 gcttgcggat tctaaaatgg cttgcgcgat cataacagaa gcgtataaat cattttcttg
     5881 tccaatttta cgagcagatt caccgatttt acggataaac gtttctacag acgcgtcttt
     5941 ttcaaaatga atagtccctt cgttatcttg tgtgctggaa gggactaatt cttgtatcgg
     6001 tgcgcttggt gcttggtggt agttttttgt ctcttctgaa ccgattgatt gtgcctgcgg
     6061 tacgtcattc ataggtacag tcacactctg ttcaggtgcc ttttgtgggc gttccgtttt
     6121 tgttggcggt tccgcttctg tttcggtact tggtttttga ggagtctgtg agctttcatc
     6181 agaaggttgc tcatttggtt cttctgacgg ttcctcctca ggttgttgat tgtcttctgg
     6241 tttgtctggt tgattcggtg tttctggttg ttcttcagaa cctgttggtg gagcgttcat
     6301 ttctggatta tctggtgtat ctattgtatc ttcagtcggt gtttctggtt gattttcttc
     6361 tccagtggaa tttgttgtcg tttcttcagt gatcgttgca gattgcgaat ttgtcagttg
     6421 attgttgcct tgttcattag ccaaaaccgc tggcgtatac ccaaatactt gacctactaa
     6481 taacaaagca gataaacatt tactcgtttt tttcacaaga ttgcctccat tcttcaatca
     6541 atcatctaga gacagctgat tgctgtctct agtcatactt taattgcttt gtttcatttt
     6601 acgacgaacc agaacagccc ccacaccaac aaaaagaaac agtcccaatg caattaacac
     6661 atcgttcact gtttctcctg tttttggtaa gctttgtttt ggtgtgctcg tcggcgtttg
     6721 tttgacaggg gttgtcggcg tttctttttt aatgcctttt gctgtcaatg tttgtttttc
     6781 attcttcaaa tcaaagtgac gtgcttcttc ctctttatgg tctttatcct tatagacaat
     6841 atgagttgcg accaatttct ccccgtcttt taacgtgcct gctttgtagt caaaggatac
     6901 tttaaactct tcagacgctt tagaaacttt acgtgcagtt tctaccgtac caacgactgt
     6961 atcttttcca tttttatcaa tcttatggaa ttgagtcacc cagtatttgg tttcccctac
     7021 cgtagattct ggaaattctt gcgatacatg atcgatcaac tcttggtctt tgtctggatc
     7081 aaatgtttgg tcgccattca ctgtaataaa gcgtgtttcg atttttggtt ctttcgcagt
     7141 cactgtttga tctttattgg ttaaatcaaa atgacgagcg aattcttctt cctcttcttt
     7201 gtcgttatag acaatgtgcg ttgcgacaat cgtgtcaccg tctgaaacca tgtctgcttt
     7261 gtagttgaac gtcacatcaa atgtttcttt ttcatcggtc acttttcgtg tttcttttac
     7321 cgtatctaat acagtgactt ttccatcggc agtaatttta tggaattgcg tcacccaata
     7381 tttctctgtc ccaaccgcag atttagggaa tacttgggac actttatcca ctaattcttg
     7441 gtcttttttc gggttgaaat cttttccacc tgtcgttgtc acaaacaaag tagaaatttc
     7501 tggttggtct tcttctggaa ccacgatttc ttcgtttttc acagtaattg gttgtacttc
     7561 tccgtctttg accgttacta aaatcgtttc tgtttgtcct gacgcttgcc cataatgctc
     7621 agggaacaca atttccgtaa atttatacgt accgtctggt aattttagtt tttctggttc
     7681 ttcttttgtt tcatacacaa aggacacatt tttattatgt gcagtcatac cttcaatcag
     7741 gaatttcgct cctggtaatt gtagatgatt gattgaatcg actttattga agtctacttc
     7801 acccgttttt aacaagttgt caatcacatc taattcaaac gtaagcacag gcgtcgtgtt
     7861 atcgccatag gccacagaga acgtggtttg ttcttcagaa ggttgacggt agttgtctgg
     7921 cacatttttt tcaatcaatt ggtatttgtt ttgatcgcca ttgtagaatt ttttgtttgc
     7981 cttccagtgt ccgtctttgt cagtttttac tgaatccaag acttctcctt ttttcacaag
     8041 gactgtttct ttatctggcg cagtaatatc tgttttggcg ataatatcaa attcgatatt
     8101 ttctgcagga acggtatcat aaacaggttt gccttgattc caggattctt ccgcattata
     8161 gacttctttg actttctttc cttctggaat gaactgttgt aagtcatttt ttgcagttaa
     8221 cgtcacttct ttgtttggtt caatcgtaat cgtatgagtt tgtttgtcta gaatataccc
     8281 agctggtgct ttgacttctt tgacatccaa tttattttga acatattttc cgtctttttc
     8341 atagaatgca gggatttctt ttaaggcgat ttcccctttg gcatccgttg tatattgttt
     8401 tgtttctccc gtatcatgac gtttgatttc atacgtcaca tttggaactt ttttccctgt
     8461 gacagcgtct agtttggtta gtttgaagtc gccatttttt agaactttaa tagttagtcc
     8521 agctctatta ggatctggca cataaagctt tcctagattt tgagcaacag gatctacata
     8581 ggcaaaactt gaacctaccc ctctagaatt tggatccagt gaagaaacag aaattcgtcc
     8641 gtcatttgag ttttgatttg cttttataaa caaactattt cctgattttc ttacttgaac
     8701 gttagctgtg ttttcaacaa ccgacccatc ccattgagaa aacacacctt ttgtatcctt
     8761 aacttctact tcttccccta ctttaaccgt cactgtttgg ctatcaaaag aaggatcttc
     8821 aagtaaattg tttatttttc tatctacagt ctgtttgaaa gcttcataat tagattgtcc
     8881 aatagttcca taattaacaa atgaaccacc taaaacttcc caaatatatg tttgtgctaa
     8941 tacatagtta tccattgaac cactagaata gtagccaaaa taaccaatta aagaaatctt
     9001 ttttctaata gcacttgtcg taattaaatt ttcgagtggt gtcacatcat attgaatacc
     9061 cgcactcgtg attgcgcctt cttgaatgca aaaaacatct tctccgtcaa tttgccagcg
     9121 aaccatgtaa tcatgagtat acattgttcc ttcaggagta attcgagtca tattcacatc
     9181 tttacgacca ttttcagtca atgttagatt ggtagcacgt gtctgtgcat tcttttccaa
     9241 gattgcttgc cattcttcag attcttgcgc caatcgttct tgttcagaaa aagaagtcgc
     9301 tttttgaata tcttcaatgg ttataaagta tttctcataa ccttcaggga cctcttgagt
     9361 cattacattg ggcggagaaa catcttctgt cgtgtcctgc gtagttgtct cttctgttgt
     9421 ttcacttgtg gtttcagtac tgatcgcttc ttcactactt tttgattctt cagtcttcat
     9481 tacttcttca gtagaatttg attcatttat cgtttctgca aatgccgtta cagatcctaa
     9541 catgggattg agaaatgtaa cgaaaattgc caaccaagtt gccattcttt taaacatatt
     9601 tttttcgttc ctttcatttt atttttcagt cttctaaagt aatttcttat ggacttcgac
     9661 accgaaacat ttttatcctc ctcaaaacaa aaaaagacta gaacttataa ttctagtctt
     9721 catcaaccac ttacttttct tttattttta gtagaaatct acagtgtgtt tttgtgaccc
     9781 gtcactgtaa ataataacat ccaccataaa acctttgttg taccactgcg aatttggatc
     9841 gctaacctct gcattagccc aatcataggc ttcatctaca gtgttaaata cctttccact
     9901 attaccaatc tctccaacta caattgttgg ctcttcaggt gtcgttgtgc caccatttcc
     9961 gccttgatcg cctccaccag gattgcttgg ttcttcaggc gttgttgttc ctccacctgt
    10021 gcttgggtta gaaggagttg tttctcctcc agtatttcca ccattacctg gttgtgtagg
    10081 tgtttgagta gtgccaccat tgttttctgg tgtacttggc gttgttgtac caccagttgt
    10141 tcctgtttga tctccagctg gtgttgttgg tgtcgttgta tttggtgtcg tttgtgtatt
    10201 tgaagtcgtt ccttgtccta ctggttgatt cggatcaact ttgtctttct cttgaatcac
    10261 tgtttgagcc acagtttcac ctgcgctgtt ttgtacactt gctacattgc cattaaatac
    10321 taatttattt cctgcataga ttaaatccac atttgtaatc ttattcatgt ccgctaattt
    10381 ttggacagta atatttgttg cttgactgat tgcgcttagg gtatctcccc agacaattgt
    10441 gtattcattc cctttaattt ctgctttgat ttgttccgcc gaacgaggtg tccaaacttt
    10501 gttttctgct gcttcagcat tcgttcccac agcaagacta actcctccga ctacgattcc
    10561 tagtgccaat actttcaaca ttttcattgt ttttcgcctc cgatttgaat aaatttattt
    10621 acaaaaacat agtaccaaac ttatgtctat cagtcgatat attttttgta gattttttta
    10681 ttttttgata tactaacttt ggtactaaaa taactaaaag gaatgtcaca catgcaactt
    10741 aataatttaa atgaaataca tgattgggct caaaataatt tacttaatcg taaacaagct
    10801 gccaaaatca ctggacaaaa ctatgatgct ttttctagct ctttacgtag aaataatatc
    10861 aaaccatttc tagaaatagg cgacgaacct ggtccttcca tcattcgctt atatctaaga
    10921 gaagatattg aaaaatacgc aaaacagctc gaagaaaaaa gacagaaacg tcaatgaggc
    10981 gttttctgtc ttggatttac aagtaaaaca ataataaaca cttttacaac ggaggttcta
    11041 tgaataagaa atacattgtt tcagttggtt caatcgtact gattgtactc gctattattg
    11101 ctttttctgt atccaacaaa acagaaacac agccacaaaa tacggttcaa tcgactacta
    11161 caagtcaatc tagcgatcat tccgtctatc cttcactaaa tagtttaaaa gaaacgcatg
    11221 gacaagattt aattgatctt ggttcttctc aaattactac tgtcttattt gaaaagaaaa
    11281 atattgcgtt tattactcct aaagaaggaa ctgaccttga attttctatc aaagtaacaa
    11341 ataccaaaga aaatttattc tccattcctg acattcaata tacccctaac aagttaaaga
    11401 ttggggatac gtttggtttg gctaaagtaa atgaacaata ctttgcttac tcactgaaat
    11461 aagtttctaa tagcaagagg aaaaaccctc ttgttatttt tatcacataa tcattttata
    11521 aaaacgcttc gaaaatatca acatttttga agaaactatt tttctaattt ctcaatttct
    11581 tccaagggat tttcgctaat gacccataca ggcacagatt gctctcgagg ctccccttct
    11641 tctaaccacc aacctgcata atgaacttct tcctcctcag cattaccatg ataccaacca
    11701 acaaaggatt catctacaac gaatgaaatt tcttcttctg gatcaaataa ttcatttttc
    11761 catgcttcaa ttgcttgttc tttggtgtac ttttgtttgt taaatacacc acaaatttcg
    11821 tcatctacat aaaaaatttc aaaatcaaaa ttactcgttc cttttactgg catcttaact
    11881 tttcctcctt tgactcactt caatagatat accactcatg ttcattccat tgtttactag
    11941 ttaattggag ttttatctta gggctttgtg cactctttcc aaaaacttga gcgtcgtttt
    12001 tttcttctaa ccaaaaaagg taatttctta cattcggtac taaatgtttt tcctgcattt
    12061 cttcatgaaa aacatataat tctgcttcat ttttcaacca attcattatt cattccctca
    12121 ttcacttctt cataccgcca ttcagatagt gagatttctt tttgtttctc cacttgtcct
    12181 ataaaaatcg ctgtattcaa caagctgatc acacttaata ataaagcaat gaaactaatg
    12241 gttacactta ctctttcaca ttttgtcatt ttgtttcctt ctttactcgt ttgttatttc
    12301 ttgtaatagg tctaataact ccttcaccga aatttgcaaa tggttcgcaa aaatttcaat
    12361 aaaagcactc gcacacatta ccagaccttc aactgatata tcactgcgac aataaaattc
    12421 tggagaaaca ctgatttctg attctttctt acaattgaac gaaaaaacta tttttgaatc
    12481 aaattccggt aattcttcca caactatgtt ttgggattct accaagaaag ggagtgcctg
    12541 ttctaatgct tcttctggtg tttgagcaga tagaataatt gcttcttgtt cttttatttc
    12601 attgttctca aatactttat aacttttttt cattgttagt tcctctttca ttcctctctc
    12661 aattagctaa gccgttactg gtacgccatt ctctaaattt agtatcgcta atccttaatg
    12721 ctcgtcgaat atcaatcatt cgataaccta ttgcacgaag ttctttatat cttttagcag
    12781 ttagcgtctc taacgtcatt tcaggcaatg ggttcgaatc aaaaatagaa ttcgaaccga
    12841 ttatttgggc caattgttgt acttgtaaca gctcttctga acttaattca gtgattgctc
    12901 gattgcctat cattgcatac attttctgtc gaatatactt cttttcttgt ttagacaaca
    12961 tttctatcca ctcctatttt cattatcatt tgttacctcc acttcagtaa atccacaaat
    13021 aaatgaattc atattccaag taccattccc ttcaactaca acttttctcc tgtcctcttt
    13081 cggatattca agaatcagac cgtttaccaa tatgtttttc actactacta atttgtttgt
    13141 ataccgtgga attgctgttc cgatatactt cactttattt cctacgctaa ttgtcatttc
    13201 ctcactccta ttctttttta tccaaaactt tttcctgtcc tattcctgcc aacgaatata
    13261 cttcactgca tgcttaaaca aaacaaaatg ttgccctgtt ttatttccgt cttttacttg
    13321 ttccacatac agttcatatt tggtcacacc ttttaaaacg ccagatactt tttttcatcc
    13381 attgtcacaa ttagtttttt catcaatcga ttgatactgc actgctcatt tttcttgttc
    13441 atatagtatt cctttctttt tcaaaactag cctcttttgt ttactagttt tcatcaatca
    13501 tcaacgattt aacagatttt ttctttcgca gggtttctag cgtcagcaac cccaaactct
    13561 aaaaaagtgc ctttgctccc ttgaaaaacg agcggacgat tgtactcgtc atcatatgtt
    13621 tttcatacta cctgatccac gcaaataacc tttttttgtt ctgtgccaca cccatccata
    13681 cgtatcgctt gagattcacc atactttttt aggtggttta tccttcctaa gtccctgtta
    13741 gacacgctga ttaacttaga aagtcctgtt tgtatccaat tttcaaagat ctcccaaaag
    13801 aaataagtag gtttacgtgt atcgcttctg ttcaaagtac ctgtttgtac ttgagtattt
    13861 ctactcccct cgttcaaggg attccttcca ccagctagct tttcctttca tcatcaaatg
    13921 ttcctgaaaa gcatttacgt ttcgtgtccg tgtgcttact tattcttttt tcgatagcga
    13981 ttatccgctc gctactcgct attttatact ttgtttgtta gctcaaataa aaactaacaa
    14041 acaaaaaagg ctagtttctt tccgttttat ttttttaact gtcgataaat agttgttagt
    14101 ttaattccaa gttctaaaac caatacgccc aacaaaacca caggaaaaac aattggggca
    14161 aacaagtaaa atcggatagt tttcatgtct tccctctttc tttttggtgt cgctgtggtc
    14221 gttcttgccg ttcaatactt cgatacattt tttattcatt atttgctcgt tttgttttcc
    14281 tctggactgc ttttcttgct ttttgaatac tttatacgct tttcgtgcct gaattaatag
    14341 gtcttgtcgg cgtttcattc gtgagaaaac aatatttgaa accatcaaat cctggttcga
    14401 acaaccacga ttcgcaataa ttttcccatc aaaaaaaata gttttcactt gagaaaaaaa
    14461 cagttggaaa tcttgttttg ttgctaattt ttttgtcaaa atcactgtgc ttttccctgt
    14521 tgaatctaca atgattcttc gaacaatccg attcgataac ttccaacgtt gccgattaaa
    14581 caatgttact tcacaacaaa tatgacgtaa gtgattaggg gtcattttca tgtgtccaat
    14641 tcctccaacc aattatgcat cgaaattttg ggcaattgtg acagcccata aatctgcgtt
    14701 tccaggtctt cataactaca agaacgcttg gcttgaagta atgaatattc tgcttgatct
    14761 gatagttttg ttgccattgg atctagattc aattctctta cacaacattc ccaaaaattc
    14821 aatagcgctc taaaataaaa tccttttcga ttctgaattg cttttccttc atgacgagca
    14881 attttttcat tttgaataca ccgttgatac gttttgctta aatcttctgc aaatgtttcc
    14941 cctaaaattc gatactcgct tccatcagat aaaccatgtg tttgattata ctttcgttgc
    15001 cgcttttcta taattcgttt actgtcgtat atcatgttta gctgctcttt cgcttcacgc
    15061 cattcagctg aactgcgaac aatccatgtc aaaacatctt tcggaacaaa cgtactttcg
    15121 ctgtaatgag caataaactc tttttgccaa tcatcagcca tttcaacagg actagtcgta
    15181 tcgatttttg tatctagatt ttctgtatct agattgttag aagaattatt aaataattgt
    15241 gcagaatttt cacatggatt tttttcgtca aaatcatcga aaatcgctaa attatcaaca
    15301 gattcctcct cttcttttcc ttgtgcagaa tctacactgg ttgagtcaac aggacctaat
    15361 gaatacacat cattttctgt cgtatctaat tctcctagat acaaccgagc tttgtttctt
    15421 tttcctgttt gttgatcaaa tcccatatcc tctatttcta gtagctcata ttctttcaat
    15481 tcttttttga ttgtattaac agaacgtctg caacaattca atgcattggc taattcttgt
    15541 tccgtaaatt caaaataaac aaaaccttga ctatctactt gatttcgttg aatggctaaa
    15601 tctaagcgtg accgaaaaac catatacgcg atttttgctt tatcagacaa tgagcgatat
    15661 ttttcacttg taataaatac ctttggaaat tgatagtata attcattgta tttacgagtt
    15721 gcggtaaatc tttttgttgc catcgttatt acacctcatc ttgtccatat attaagaaag
    15781 aattttggtg ttggtagaca cgaacatctt ccattgtagc aatcaattct tttagataaa
    15841 ttcgaccgaa ttcaattgtt agtaagtcgt aatcttctaa ttcatttttg atttcttcaa
    15901 tttcttcttc agacacattg agtaaaatgg ctaattcttg attggtataa gaaatataga
    15961 catgtttctt ttcatcaatc cgtttttcaa ttaaggattc ttctaagtac tcattcagta
    16021 agctataggc gtattttgct tgtgcagtca tcgcataata ttttgacgat tttataaaga
    16081 atagttttgg catatgaaaa gttgcttgtg tcagcacatc tgttgcgcga aatttgtttt
    16141 ttaccatatt ttttcctcct agaaaaacac gcaaaaaaag ctctgtataa atcgaattac
    16201 acagagtttc ttcttgttga tatttaattt ctaaaatacg tttcgggtaa aaactaaaca
    16261 cttactaata tttagattgt agaatcttcg ccttcattta ttcattttaa agagaaaaag
    16321 atcaagtgaa atgtgcccaa aagtttgatt ctatt
//
LOCUS       DAYYCY010000051        16170 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000051 DAYYCY010000000
VERSION     DAYYCY010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16170
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.051"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(188..814)
                     /gene="pyrE"
                     /locus_tag="ACHAZB_001310"
                     /pseudo
     CDS             complement(188..814)
                     /gene="pyrE"
                     /locus_tag="ACHAZB_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
     gene            complement(821..1537)
                     /gene="pyrF"
                     /locus_tag="ACHAZB_001311"
     CDS             complement(821..1537)
                     /gene="pyrF"
                     /locus_tag="ACHAZB_001311"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFM8613996.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            complement(1534..2466)
                     /locus_tag="ACHAZB_001312"
     CDS             complement(1534..2466)
                     /locus_tag="ACHAZB_001312"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HFM8613997.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(2463..3239)
                     /locus_tag="ACHAZB_001313"
     CDS             complement(2463..3239)
                     /locus_tag="ACHAZB_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HFM8613998.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(3293..6478)
                     /gene="carB"
                     /locus_tag="ACHAZB_001314"
     CDS             complement(3293..6478)
                     /gene="carB"
                     /locus_tag="ACHAZB_001314"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFM8613999.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(6483..7559)
                     /locus_tag="ACHAZB_001315"
     CDS             complement(6483..7559)
                     /locus_tag="ACHAZB_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HFM8614000.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(7564..8844)
                     /locus_tag="ACHAZB_001316"
     CDS             complement(7564..8844)
                     /locus_tag="ACHAZB_001316"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFM8614001.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(8863..9789)
                     /locus_tag="ACHAZB_001317"
     CDS             complement(8863..9789)
                     /locus_tag="ACHAZB_001317"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HFM8614002.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(9802..11085)
                     /locus_tag="ACHAZB_001318"
     CDS             complement(9802..11085)
                     /locus_tag="ACHAZB_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HFM8614003.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(11099..11638)
                     /gene="pyrR"
                     /locus_tag="ACHAZB_001319"
     CDS             complement(11099..11638)
                     /gene="pyrR"
                     /locus_tag="ACHAZB_001319"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HFM8614004.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(11873..12784)
                     /locus_tag="ACHAZB_001320"
     CDS             complement(11873..12784)
                     /locus_tag="ACHAZB_001320"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFM8614005.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(12781..13245)
                     /gene="lspA"
                     /locus_tag="ACHAZB_001321"
     CDS             complement(12781..13245)
                     /gene="lspA"
                     /locus_tag="ACHAZB_001321"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFM8614006.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(13250..13948)
                     /locus_tag="ACHAZB_001322"
     CDS             complement(13250..13948)
                     /locus_tag="ACHAZB_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFM8614007.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(13935..14321)
                     /locus_tag="ACHAZB_001323"
     CDS             complement(13935..14321)
                     /locus_tag="ACHAZB_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8614008.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(14468..16138)
                     /locus_tag="ACHAZB_001324"
     CDS             complement(14468..16138)
                     /locus_tag="ACHAZB_001324"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HFM8614009.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
BASE COUNT         4352 a         3730 c         2884 g         5204 t
ORIGIN      
        1 ctcttctttc tgctaaccag tcttagaaaa atgaatttta aaaaaatcaa aaactgatca
       61 cgatagaagt ttcttttcat acctgatgaa aagatgcttc tcgcaatcag tttttggatt
      121 tttgctattc caattgatga ttctggagac acaaatcgga tttctctatc ttcatcaagc
      181 gcgcttcaat ctgccaattt tcaggatctt ttttccattc ttttagcaga gttaattctt
      241 gctcatcgat ataacgatct tctaaagctg cttcaatcaa tacagaataa ttggtcaacg
      301 tcataagagg gatttcggct ttttcgaaat ttgcttttcc ttttggcaac tcatacgtaa
      361 agatagccgc aacaccaagg atatccgcac cttctctttt tgctgcttca gctgcttcca
      421 agacacttcc gcctgtagaa atcaaatcct cgatcacaac catcttctga ccttcaaata
      481 tacgtccttc gatttggtta cctttcccat gatctttcgc cttacttcga atatagacca
      541 ttggcaaatc cagtatttcc gctacccatg cggcatgagg gattcctgct gttgcagttc
      601 cagcaatcac ttgtacttca gggtaagctt ctttgatttt gcttgctaat ccttttgcga
      661 tctcttttcg gacttttgga tagctcatcg tgattcggtt atcacagtaa attgggcttt
      721 tgattccact tgcccatgta aaaggattac ttggatttaa aaatactgct tcaatttcta
      781 ataagtcttt tgcgatactt tgttcaacat tcattttagg ttagctccat tctgtttgaa
      841 tcgtttgata tgctttataa ggattagttg actgagtgat tggtcgacct ataacgataa
      901 aagttgagcc gatctttttt gcctgagaag gtgtgactac tcttgtctga tctcctgcga
      961 ctgttccttc tggtcttata cccggagtta agcaaacaaa ttcgtctcct gccatttctt
     1021 tgatcgcttc tacttcccaa gcagaagaaa ccacaccatc taatcctgct tttttggcac
     1081 agctagcata atggatcaca cttttttcta aagaaacttc gattagttga tcggcatgca
     1141 tttcttcttc acttgtagaa gtaagctgag tgatagctag aagtagcgga cgttttccac
     1201 cttctggagt cccctcttct aatcctcgca ttgccgcctc catcatacga ataccgccag
     1261 cggcatgaac acaggtgaga tcaacaccaa gctttgccaa tccccgcatt gctttttcga
     1321 ccgtattcgg aatatcgtga agtttcagat ctaaaaatac attatgacca gcctctttta
     1381 gataacgtac gatttcaggt ccttcttggt agaacaattc cattccgatt ttcacaaaca
     1441 atttttcttc tttcgggaaa aatcgaagaa agtcttctac ctcttgccta gttgaaaaat
     1501 ctaatgcaat aattggtcgt tggtctagtt gattcatcgt tcacgttcct ctctcacttc
     1561 tttgattaat ctttccagcg attcgatgcc tagttcgctc attcgcacag ggagctcttt
     1621 aattagttta ggacagatat acggatcggt gaaattcgcg gtccctatag ctacagcact
     1681 tgctccagcc ataaacatct caagaacatc atccactgtc tgcacgccgc ccatcccaat
     1741 gattggaagg ctggatatac ttgctacttg gtgaatcaaa cgaatagcga caggcttgat
     1801 tgctggtccg gagagtccac ctgtttgatt ggctaaaatc ggttttcttg tcttaaggtc
     1861 aatccgcata ccaagaagag tattgatcat cgtaaaccca tctgcccctc cagcttcgat
     1921 tgcctgagcg attggaacga tgtctgttac atttggggac aatttgacat agacaggaac
     1981 agaagaaact ttctttactg cttgtgttaa ttgaaaagca acatcagaat ctgttccaaa
     2041 agcaattccg ccatgtttga catttgggca agaaatgttc agctcgattg ctttcacatt
     2101 tggtgcttcg ccaattttgc tacaaacttc tacatagtct tcttcacaag caccagcaac
     2161 atttgcgatg atgggtaact cattgtactc ctctaattct ggaagaagtt tatgcataac
     2221 aacgtctaga ccaggatttt gcaaaccaat tgcattcaac atgccagatg gtgtttccgc
     2281 cactcttggc gtgggattgc caaagcgggc atttggagtc gttgccttga ccatgatcga
     2341 tccaagctga ttcaaatcat agtattttcc gtattctttg ccaaaaccga aacagccgct
     2401 tgccggcatg ataggatttt ttaattctag gccaggaagt ttgaccgcta aaggattctc
     2461 attcatataa ttacctctcc tattttaaaa atcggtcctt cgtcacaaac tttcacactt
     2521 tttgttcctg tttcatcttc cggcgtatga caaacgcaag catagcaagc ccctatccca
     2581 catgccatgc gggattctaa tgataactgg acattttgat gcgaagaaaa cagctgttcc
     2641 actgtcttca ataacccgtt tgaaccacat gcaaaaacag ctgtaggttc cgtcttcgtt
     2701 cctaacaata aattaccgac atttccgtgc ataccgtatg ttccatcgtc agtagcgatt
     2761 cttgtttctc ctagacgatt aaattctttt tcataataaa cgacttcatg tgtggcaaaa
     2821 cctaaaaaat gcgttggctt aattcctttt tcgactaact gttttgataa ctcatataga
     2881 ggagggacac cgattcctcc accgattata aatacttcgt ctcctgtatg aagattggac
     2941 aaatcaaaac cattgcctaa aggacccatg acatctaaat ggtctccggc agaaagagta
     3001 gagaagactc tagtcccctc accttccacc cgataaatga tacggcaagt atttgtttcg
     3061 tggtcgatat gattcaagct aatcggtcgg cgtaatagca agtctgcacg cggcacgaga
     3121 atatgcaaga actgtcctgg ttgctgcatt tcttttacta gatcaccttt caatgtcatt
     3181 tcaaaaatct ttggtgccag ctgtttttgc tgaacgattt ccatcatttc ttgtttcatc
     3241 tgtcccctcc tgcaacggtt tttcgtcttt ttgtcttaac atgtgctcgc cgttaaatag
     3301 ggttagttga aaaagcacgg gattccatca ctcgaacgat cgcatctgct gtgtctaaag
     3361 aagtaaacaa tggaatacca tgttcaactg cttctcttcg gatgatgaaa ccgtctttag
     3421 aagcatttcc ccgatcttta tcaatcgtat tgacaaccac ttgtgtttta ccatctctta
     3481 ttaagtcgac tacatttttt tctgctgctt cctcagaaat tttcgctact gtttcaacga
     3541 ccagaccatt tttttcgaaa taagcagctg tatgtttcgt tcctatcaga ctatagccaa
     3601 tttctgtaaa acgtttagct aattctagtg cttcttcttt tgtttcgtcc gcaatcgtaa
     3661 ataatacggc tccatattca ggcaagtgta atccagaagc ttcgaatgcc ttgtacaaag
     3721 ctttgtctaa ttgtctgtca gatcccatta cttctccagt cgatttcatt tcaggcccta
     3781 agtaagtatc aactttctgc aatttcgtaa aagaaaagac aggtgcttta acatgaactt
     3841 cttttgtttc tggataaagt ccgttttcat aaccaagtgt ctttagtttc tctcccaaaa
     3901 tagctttcgt cgcaatttgt gccattggaa ttcctgtcac cttacttaag aatggaacag
     3961 tccgacttgc tcgaggattg acttcgatca cataaacctc attttcatgg atgacaaact
     4021 ggatattcat catcccgaca caattcaaac ctaatgctaa gcggatcgtg taatcttcga
     4081 tcgttttctt gatcgattca gataatgttt gaggtggata cacagccatt gaatcccctg
     4141 aatggacacc agcacgctca atatgctcca tgatccctgg gatgagtact gtttcaccgt
     4201 cacaaatcgc atccacttcg cattcaccac caagcaagta acggtccact agtactggat
     4261 gttctggaga tgctttcaca gcatgttcca tgtaatcttc taaatctcgc tggttttcaa
     4321 cgatttccat cgcacgtccg cctaatacat agcttggacg aacaagaact ggataaccaa
     4381 ttctcgaagc gataaccacg gcttcttctt tacttgtagc agtatcccct aaaggttgag
     4441 gaatatccaa ctctttcagc gcttgctcga ataaatcacg attttccgca cgatccaagt
     4501 cttcgatcgt tgtccctaag atttttacac cagcagcagt caaaggttcg gctaagttga
     4561 ttgctgtctg accaccaaat tgaacgatta ctcctatcgg ctgttctagc tcgatcacat
     4621 tcatcacatc ttcgaaggtc aatggctcaa aatataattt atccgaaata gaaaagtcag
     4681 tcgaaactgt ttcaggattg ctgttcatga tgattgcttc gtatcctgcc tgctggattg
     4741 ctttgacaga atgcacagtc gcatagtcaa attcaacccc ttgtccgata cggataggac
     4801 cagatcccag tactagaatt gagggctttt ctgatcgtga actttcattt tcatattcat
     4861 aggaactata aaaataaggt gtttttgatt cgaattccgc tgcacaagtg tcaaccatct
     4921 tatatacagg aatcaaatca ttttcttttc tcatctggcg aacggcttct tcgtttattt
     4981 cccacagctc cgccactttt ttgtctgtaa aaccgttttg tttcgctgtt ttcaatatct
     5041 tgtcatccaa cggctgctgt ttcaactgct gttcgatttc tacgatatgc aacagtttat
     5101 ctaaaaagaa taaatcgatt tttgtcaaat ccgctaattc ttctatggtg taccctcttc
     5161 taatagcttc agctacataa aacaggcgat catcttgtgc tcgaaccact ttttggatta
     5221 ggtcatcgtc cgtgatgtct gccaactcag gaagttcgat atggtgtgta ccgatttcta
     5281 aagaacgcac agctttcaat aaagactctt cgatattccg accgatagcc atcacttcac
     5341 cagttgcttt catctgcgtg cctaaaagcc gttctccgtt ttcaaactta tcaaacggcc
     5401 agcgaggaat tttagctaca acataatcta atgcaggttc aaattctgca aaagtcgttc
     5461 ctgtaacagg atttttcatt tcgtctaatg ttaatccaag cgctatttta gcagctagct
     5521 tagcgatcgg atagccagtc gccttacttg ctaacgcaga tgaacgagaa acacgcggat
     5581 tgacttcaat cacataataa ttaaaactat gtggatcaag agcgagttgg acattacagc
     5641 ctccctcgat cttaagtgct cgaataatcg aaagagaagc atcacgaagc atttgatatt
     5701 catggtcaga caatgtttga ctaggggcaa atacgataga atcacctgta tggataccca
     5761 ccgggtcaaa attttccata ttgcagacaa cgattgcatt atcagccgaa tctctcatca
     5821 cttcatattc aatttcttta aaacctgcaa tacttcgttc aatcaaacat tgggtagcgg
     5881 gggaaagact taatccattt tctgatacga tacgtaattc ttcttcattt tcacacatac
     5941 ctccaccagt tccgcctaaa gtaaaggctg gacgaacgat gacaggatag ccgattttat
     6001 ttgcaaaatc tacagcttct tctactgtat tgacgatttc agactcgggg attggctgat
     6061 tcaattcttt catcaactgt ttgaatagat cacgatcttc tgcttgatcg atcgcagaaa
     6121 gctttgttcc tagaagttct atgttcaatt cttctaaaat acctgatgct gccagctcca
     6181 ttgccatatt gaggcctgtc tgtccgccta aagttggaag gagagcatct ggacgctctt
     6241 ttcgaagaat ccttgataca aattcaagtg tgatcggttc gatatatact tgatctgcca
     6301 cttctttatc cgtcatgatc gtcgcaggat tagaatttac taaaaccact tcatatcctt
     6361 cttcttttaa ggcaaggcag gcttgtgttc ctgcgtagtc aaattctgcg gcttgaccaa
     6421 tgatgatcgg accagatcca atcaccataa ttttcttgat atctgtacgt ttcggcatct
     6481 gtttactgct cctttcctgc gtccatcatt tccataaatt catcaaataa atgtagtgca
     6541 tcatgcggac caggggcagc atctggatga aactgcacac tgaaagcggg ataatctctg
     6601 tgtttgactc cttctactgt tccgtcattt acttctacat gagttacgat gagtttttct
     6661 ggatcaatcg tggcttcatc gactgcatag ccatgatttt gagaagtaaa atcaattctt
     6721 ccggtcgcta cttctcgtac cgggtgattt aatcctcgat gtccgaattt cattttatag
     6781 gtatctgcac cgttggctaa agcaaataac tggtgaccga gacaaatacc aaaaatcggt
     6841 acttttcctt ggattttttt gatcatttcg attgcttctg gcacatcttt tggatcacca
     6901 ggtccattcg tcagcatgac tccgtctggt gatagcgcta aaatcgtctc ggcatctgtg
     6961 ttatagggaa gtactgtgag gttacagttt cttttactca attctcgtaa aatactatgt
     7021 ttcaagccga agtcaatgac aatgactttc cgacctgttc ctggacttgg ataaggtttg
     7081 ttagttgaga cttgctgcac ttgattggtt gctagaacgg ttgctttcag ctgatcaaat
     7141 gcatgttcga aggaatctcc agcatcaact atactagctt tcatcgttcc gacttcacgg
     7201 attttgcgag taagggcgcg cgtatcgata cctgatattc caggaattcc tttttgtttc
     7261 aaaaatgaat ctagcgtcat ttggtttcgc cagtttgaag ctacacgtgc atgttctttt
     7321 actactactc ctttgcatgt tggagaaatc gattcataat catctctatt taccccataa
     7381 ttacccacca taggataggt aaatgtaatg atttgaccgt taaaactttg atcagtgata
     7441 gtttcttgat aaccagtcat acttgttgta aaaacgattt ctccgaagac actagcatct
     7501 gcaccaaaag cctttccttc gaaaactgtt ccatcttcta atataagcaa acgttccata
     7561 cttttattct cccttctgcc acacaagttt tccgttgaca aaagtcattt gtgtttcgcc
     7621 ttttactttc cagccgataa aaggtgtatt ctcccctttt gataaaaagt cttctttatc
     7681 gatcgtgcaa gtttgtgtaa tatcaaaaac tgctacatca gcgggggcac caaccgttag
     7741 cgttcctgca ttcaaaccaa aaatctcagc cggtttaact gccaaccaat tgatcacttg
     7801 ttccaacgtg aatcgaccag tttctacaaa atgagtatag atcagttgaa acgctgtttc
     7861 gcttcctacg ataccaaatg gtgatttcat gaaactttgg cttttttctt ctaatccatg
     7921 gggggcatgg tctgtagcga tgcaatcgat cgtaccgtca agcaagcctt cgatcaaagc
     7981 ttctctgtcc tcccgtcctc ttagcggcgg gttcattttc caaaagccga aatcttcggg
     8041 aatatcttcg tcgatcaaaa taagatgatg gggtgaaact tctgctgtta cgtggattcc
     8101 tgcttttttt gcgtctctga ttacacggac actttcttta gtagaaacat ggcaaacatg
     8161 gtaatgaaca ccagttgctt cagctagcaa cagatctcta gcaatttgag aagactcggt
     8221 tacgcttaag atcccgggaa gaccaagttc ttctgccttc ttgccagcgt gcataacgcc
     8281 gccaaataat aaagattcat cttctgtatg ggctactagg gctttattgt tttttgcagc
     8341 ttctttcata gccagataca tcgtcccagc tgtttggaca cctacaccgt catttgtgaa
     8401 agcaaaagca ccttcttcaa tcagtgcttc ttgatcaact aatttctcac ttcgcagatt
     8461 ttctgtgatt ggcgcatact gcaatacctt cacaactgca tcttttctaa tgatgtcata
     8521 tacttgtctc agcttttctg ccgtatcagg tacaggattt aaattcggca tcgcgcatac
     8581 agtagtgaaa ccgccacgag cagctgagcg agtcccagct tcgatcgtct ccttgtatgt
     8641 aaatcctggc tctctcaaat gaacatggac gtccactaaa ccaggtgtga tcaattgtcc
     8701 ctttgcatca aacacttcat caaattcggc ttctgagaat cctgtaccga ttgctttgat
     8761 tcgcccatct tctatccaaa tttctgctgg tgtcgtcata ttttttcgtg tattgatctg
     8821 accgttttta atgagtgttt tcattttttc atcctcctat aattacgctt ttccatttag
     8881 cactgcttct aagattgcca ttctgacgaa tactccattg ctcatttgct gaacgatccg
     8941 cgactgtaat ccttccacta atgaatcagc taattctaca tcacggttaa ctggtgctgg
     9001 gtgcataatg attgcatgtt tttgcaactt cttcgcacgc tcaactgtta acccatgttc
     9061 ctgatggtag ccttcttttg agaaactttc ttttccatca tggcgttcgt gttgcacccg
     9121 aagcatcatc atgacatcaa cttgatctag tagttcatcc aatggcatat aatgtccgta
     9181 cacttcgaat tcatcgtcgt accattctct aggacctgag aagaatactt gtgcgcctag
     9241 acgttttaac atctgcatat tggatttagc tacgcgagag tgagtgatat cacctacgat
     9301 ggcaactttc agaccttcaa aatgaccaaa ttcttcataa atggtcatca aatcaagcaa
     9361 gcattgggtt ggatgctgtc cgcttccgtc tcctccattg ataatcgaac actggattgt
     9421 tttactttgt atcagttcgt cataatagtt ctcatcacca tgacggataa ccgctacgtc
     9481 tacgcctaat gctgacatcg tcagtactgt atcgtatagt gtttcccctt tttggacaga
     9541 gcttgtgctt gcttcaaatt caatcacttc tagccctagt tttttctccg ctacatcgaa
     9601 gcttttatgt gtgcgagtac tattttcgaa aaagaggttt gtagcaaagt actggcgctt
     9661 ttgcggagtc caattagcac cttgcttgaa ttcctgccct ctgcggatca agcccataac
     9721 ttcttgatca gtaagtgcct caacggtcaa taaatgcttt aagctgatac gttctgattt
     9781 gatgatcata atgtttcctc cttagttttc ttcgcttcgt gaagtttctg gtaagataag
     9841 atttaaaatg atgccaagta cagttgctaa tgccattgcc gacaatgtaa atgtgcctac
     9901 ttcaaagacg agtccgccaa ttcctactac taagataact gaagcaatca acagattttt
     9961 ctttttatca aaatcgattt tgttctcgat taaaatcttc attccacttg cggcaattac
    10021 tccaaataag ataaaactga ttcctgagat gactggtcca gggatactta aaatcaaagc
    10081 gcttaatttt cctacgaatc ctaaaatgac tgcaaatact gcagctccac cgatcacaaa
    10141 cacgctatgg acacgtgtga tagccagaac accaatgttt tcaccgtaac ttgttacagg
    10201 aggacctccg acaaaggctg ccacgatttg tgctaatcca tcacctgtca acgtcttatg
    10261 aagacccggt tcttcaaaga aattacgttt agttagttta ttcaatacca taagatggcc
    10321 gatatgctct gtcatcgtca caaaagcgat gggtgccatt gttgtgattg ctccaagata
    10381 tagctttggt tcatattgta caaagggaat ctcaaagtta ggaacagcaa accaggcagc
    10441 gtctacgatt ggcttagtat ccacgattcc aaacagcaag gctacgatgt atccgctaat
    10501 aatacctagt aaaatcggaa tcaatccgag aaaacctctt agccacatgt tgaagaaaat
    10561 tgttaatcct aatgtgatca gcgctacagc aatatacttg aaatcatacg tgccctgatt
    10621 atacatagca tttgttgctg catttgccgc aagtcccaac ccgataacca tgatgactgg
    10681 accgacgacg attggaggca agattttatc cagccatgct gaaccgatct ttttgatgat
    10741 cagcgcaaca atcaaataga ctaatccagt tgtgactgct ccttgtgcaa tcgctggata
    10801 accatctgtt ttcatcaaaa gctgcatcgc tgcaatgaaa gcgaagctgc ttcctaagta
    10861 cgcaggtatc tttcctttag tagtaaataa gtacaccaat gtacctagtc ctgaactgac
    10921 tagcgcaata cctggatcga tacctactaa gatggggact aacacggttg ctccgaacat
    10981 cgtgaacaag tgttgtaaac ttaatcctac ccaatgtcct ggggttgggc gatcgtggat
    11041 atctaacacc acgtcatcat tatgaaatcc ttttttctcc gacatgtttc tctctcctct
    11101 aatcttcctc tttcaaaatc atgatgcgat cttgtccatc aagctcttgc atctctacta
    11161 aaatctcttc cgcccgagat gttggaatgt tcttcccaac ataatctgca cggattggca
    11221 attctcgatg cccacgatcg accaggactg ctaatgaaat ttttcttggt ctgccaaaat
    11281 ccattactgc gtccattgct gctcgaatcg ttcgtcctgt atacaaaaca tcatcaatca
    11341 aaataacttc ttttccttct aaggaaactg ggatgtcaga agaatgaagt tccggttctt
    11401 ctggattttc ttttttgtca tctcggtata aagtgatatc cagttctcct acaggaatat
    11461 cgatatcttc tagctgcttc aggcgttcgg caattcttga tgcaatgtag attcctcgtg
    11521 ttttgatacc taccaatacg atatcttgga tactatggtt tctctcgata atctcataag
    11581 taattcgagt caatgctcgc ttcatggtta cctgatctac tacttctttt gcttgcattt
    11641 tcattccccc tacttttttg cataaaaaaa ctcctgccct aagaggacag gaggaatgtt
    11701 tagactgagc gtatactgat acactgatag ctatactatg tcaatgagca atctacttat
    11761 ggcgcaccat acgtatcctt gctcccaccc ttcttagcct cacgggacta aacttaaagg
    11821 aattctatta agttttatgc tttcgttgtt aattactata ccgttatcta aattaaaatg
    11881 caatgttttt tcttaatttt tctaaagttt tttcaaaaac ttctggaaga ggtgcttcaa
    11941 agatcatctt ttcgtttgta gtcgggtgcg taaaacctaa catttgcgca tggagaaact
    12001 gaccattgcc tttcaaagtt ttcttcggtc cgtatactgg atctccagct aacggataac
    12061 cgatatactt catatgcaca cggatctggt gtgttcttcc ggtttctagt tttaattcaa
    12121 ccagagtaaa tccttcgaaa cgttccaata cagtaaaatg tgtgacagcc gacttcccat
    12181 cttctcttat cgcctgcatt ttccggtttt ccttcgaacg gccgattggc gcattgatgg
    12241 ttcctttttc gtgaggaatc tctccatgga ctaaagcaac atatttgcgc aaagaggtct
    12301 tatcttttaa ttgctgagct aatgattcgt gtgcctggtc atttttggca accattagca
    12361 gtccggaagt atctttgtcg attcggtgaa caatacctgg acgaataacg ccgttgatcg
    12421 tagaaagatc cttgatatgg tacatcagcg cattgaccat tgtgccatgc gtatgacctg
    12481 ctgaaggatg aacgaccatt ccttgaggtt tgtttatcac cgctacatct tcatcttcgt
    12541 aaacgatcgt caacgggata ttttctggga ccacatctaa tgtttctggt tcggggatca
    12601 agacagtaat ctcgtctcct tgttttactt tgtaatttgc tttgactggc tgtccattga
    12661 cagaaaccgc tccatttttc agccacagct gaatttgcga acgactgtaa tcggtgaata
    12721 aattagtcaa tactttatcg atcctaccag attcatggtg aatcgtcgtt ttaagttctg
    12781 tcattttttt ctttgatcct tttcatctaa aataatataa attaacatac aaatcactcc
    12841 acatacaagt gtcacatctg ctacgttaaa aatcggaaag ctcataaaat ctgtctggaa
    12901 catatctact acatagccaa gacgaatacg gtcgataaaa ttgccaatcg caccagctag
    12961 aatcaaactc aatccaatca tgaaccagcg attacctttt tcacgatttt taatcatcaa
    13021 tgtgagaatc acaacgactg caatgagtgt cactatagta aagaaccaca tcttcccttc
    13081 tagcatactc catgcggctc cagtgtttcg aatatgattc agtgacaaaa tacctggtat
    13141 aagatttttc gtctctccaa gcgctagatt atcagtgatc caccatttga ccaactgatc
    13201 cactcctact agcaaagcgc tgaatatcca ataaataatt aacaaaaaat caatcctttt
    13261 ctatttgttt ataaatatca cgaggtcgta cgatcccgag cagattacca tcgggattcc
    13321 catctttagt gataagtacg gcttccaaag tcccttgctc acgaaacatc gcttcaacct
    13381 cgtaaatatg tgttttagct ggaacaaaat gataatttgt gtactttccg ttttggatga
    13441 tcaaatccaa cgctcgttta tttgttaaat caaccggacc tttttggctt tcttttgcta
    13501 gccaaaaccc gatattacgt aaagtaatca atgctacaaa ttttccttta ttgtataacg
    13561 gaaattgtga ataccttttt tctgctacta tctttaacag ttcaagtaac ggaagatctt
    13621 gttcaaaacc tgttacacgt tttgcaaatc gaggcagcac ggtttcaggc tgaatcagtt
    13681 cttgttcgat cagctggatt cgcttgactg tccaatcatt tggctctgca atgacaaaat
    13741 cgactgcgat ccgatcatgg acgatcgcat tccttaactg agcaagctgt aaaagatcat
    13801 cttcgtattt tctgatcatt aattgttttc tttgagctag gcgtctgaca agttcggtaa
    13861 atcccatatt tcgtgcattt cccatctcgt ctctcatcca tttttcgatt cgattgaaac
    13921 tgattaaaaa aagatcagag tttgccataa ttttcctcct tgatcaaata tgcccggttt
    13981 tttatataga aaaagaacgc aatcaacaca ataactgcaa aaaaactgaa aaataaattt
    14041 tgtaggaaaa cggcccccaa cccagttgct agaaataaaa acggatagat ttgaagaatc
    14101 aagtaattca ttgtgatcac ataagcaggc tttgcttctt tcactgtata aacggggctt
    14161 tttagccaat aaaaggtgac ctgcatcgta aagggcactt ccagatcaag cgttatcttt
    14221 tgttcatgac tgagaacact gcgtttttca ttgttgattc ttagctgaat cgagctaccc
    14281 ataccgtaaa aaccggtttt tcttataaat gtcgttttca tcataccctc ctattatgct
    14341 ttagtatagc tcaaaataca ataaagaaaa agaatgatcc tagtgtaaac aaaattaatt
    14401 ccctatcgtc taaaaaaaga agctgtagtc aaaatacagc tccttcgatg gtagattata
    14461 atcaagctta aaacaatccc aacacttctc cgtcatttgt cacgtccata ttcaatgcag
    14521 caggtttttt aggcaaacca ggcatcgtca taacatctcc agtcaaggct actaaaaatc
    14581 ctgcccctaa ttttgggatg atttcgcgga tcgtgatggt gaaatcttcc ggtgcaccta
    14641 gcagtgaagg atcatcagaa aatgaatact gtgtttttgc catacaaatc ggcagtttgt
    14701 cccatccatt cttcgtaaac tcttctattt gtttttgcgc ttttttgctg aaaacaacac
    14761 catttcctcc gtaaattttc ttgacgattg tttctgtctt ttcttgtatc gtttcttcta
    14821 ggcggtataa tggcttgtaa ttagcttctt ttctatcaat caatcgtact acagcttttg
    14881 ctaaatcaac gccgccttct gctccatctg cccatacact cgctctcttg gcaagaatcc
    14941 cctgattttc acacaagtgc tccaacagtg tgagttcatt gtctgtatct gttacgaatt
    15001 cattgattgc gacgatcaca ggtagctgat attgttccat attacgtata tggcgcttta
    15061 aatttgcaaa acctactttc aaagcatcaa gattctcatt tttaagttca tcttttttca
    15121 ttccgccatg cattttcaat gcacggacag tggcgacaat caccacagca tccggcgctt
    15181 ttttcaagtt aggtactttg atatctaaaa atttctctcc gccaagatct gcaccaaatc
    15241 ctgcctctgt gacaacgtaa tctgctaatc tcaatgctgt cttggtagct aaaatactat
    15301 tgcagccatg agcgatattg gcaaacggtc ccccgtggac aaaagctggt gttccgtaaa
    15361 ttgtttgtac aagattcggc ttgattgcat ctttcagcag taatgccaga gcgccttctg
    15421 ctttcaagtc tccagcagtc acaggctgac ggtcaaatgt atagccgaca acgatccttg
    15481 caagacgagc ctttaaatct tcaaggtctg ccgcaagaca caagatagcc atgatttcgc
    15541 tggcaactgt gatatcaaac ccatcttctc ttggaacacc ttggattggc ccacccaagc
    15601 caacaattac tttacgcagt tcacgatcat tgagatcgac tacccgtttc caaatcactc
    15661 ggcgggcatc gatgttcaat tcatttcctt gatgaatatg attatctaaa agagcagata
    15721 atgcattatt agctgttgtg atagcatgca tgtcaccagt aaaatgcaag ttgatatctt
    15781 ccataggcaa tacctgtgca tatcctccgc ctgcagcacc acctttgatt cccatcacag
    15841 gaccaagtga gggttctcgc aatgcaataa cagatttttt attgatttta ttgagcgcgt
    15901 ctcctaaccc tacagtgatc gttgactttc cttcgccagc tggtgttgga ttgatggaag
    15961 taactaagat caaatgcccc tcagcgttat cctttgatcg attgattcca gaaaaattga
    16021 ttttcgcctt atatttccca tacagttcca aatcgtcttc cttaagatct aatttactag
    16081 ccacttcaac aatcggttct agctctgttt cttgtgaaat ttgcagatca ttcttcattt
    16141 gattacccct tttcacgaac tttattcaac
//
LOCUS       DAYYCY010000052        16079 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000052 DAYYCY010000000
VERSION     DAYYCY010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16079
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.052"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            165..1310
                     /locus_tag="ACHAZB_001325"
     CDS             165..1310
                     /locus_tag="ACHAZB_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFM8614010.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            1447..1791
                     /locus_tag="ACHAZB_001326"
     CDS             1447..1791
                     /locus_tag="ACHAZB_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HFM8614011.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            2062..3348
                     /gene="efmA"
                     /locus_tag="ACHAZB_001327"
     CDS             2062..3348
                     /gene="efmA"
                     /locus_tag="ACHAZB_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HFM8614012.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLTGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(3486..4085)
                     /locus_tag="ACHAZB_001328"
     CDS             complement(3486..4085)
                     /locus_tag="ACHAZB_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM8614013.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            4489..5610
                     /gene="mnmA"
                     /locus_tag="ACHAZB_001329"
     CDS             4489..5610
                     /gene="mnmA"
                     /locus_tag="ACHAZB_001329"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFM8614014.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            5856..7277
                     /gene="arcD"
                     /locus_tag="ACHAZB_001330"
     CDS             5856..7277
                     /gene="arcD"
                     /locus_tag="ACHAZB_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043858 - arginine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HFM8614015.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            7291..8409
                     /locus_tag="ACHAZB_001331"
     CDS             7291..8409
                     /locus_tag="ACHAZB_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HFM8614016.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(8469..9761)
                     /locus_tag="ACHAZB_001332"
     CDS             complement(8469..9761)
                     /locus_tag="ACHAZB_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFM8614017.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            9911..10471
                     /locus_tag="ACHAZB_001333"
     CDS             9911..10471
                     /locus_tag="ACHAZB_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8614018.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            10600..10977
                     /locus_tag="ACHAZB_001334"
     CDS             10600..10977
                     /locus_tag="ACHAZB_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HFM8614019.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            10910..12982
                     /locus_tag="ACHAZB_001335"
     CDS             10910..12982
                     /locus_tag="ACHAZB_001335"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFM8614020.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(13193..14140)
                     /locus_tag="ACHAZB_001336"
     CDS             complement(13193..14140)
                     /locus_tag="ACHAZB_001336"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM8614021.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            14386..14850
                     /locus_tag="ACHAZB_001337"
     CDS             14386..14850
                     /locus_tag="ACHAZB_001337"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFM8614022.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(14912..15955)
                     /locus_tag="ACHAZB_001338"
     CDS             complement(14912..15955)
                     /locus_tag="ACHAZB_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HFM8614023.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
BASE COUNT         5256 a         2931 c         3388 g         4504 t
ORIGIN      
        1 aaagaagttg tgacagaagt gagcagcttc aaggagctgc gacaagactt ttgtcacagc
       61 tccttttttc tatgcccatg aaacgtcctc tagacaaaaa tgagctcttc tttggtttgt
      121 tcgctatttt atgctagaat gtgcctagca ataatttgga agggatgaat gaagtgaaaa
      181 ctatttatct ggatcatgcg gcaactaccc ccatgcatcc acaagtaatt gaagcgatga
      241 cggcaatcat gcaagacacg tttggaaatc cttcaagcat acatggattt ggtcgccacg
      301 cgcatgaaaa attggaaaca gcaagacaaa tcattgcaga cagtctgcaa gcaaaacctc
      361 acgagatcat ctttaacagt gggggaacag aaggggataa tacagctatc ttagaaacag
      421 cgttctcgcg tcaaaaagaa ggcagacatc taatcactac agctatcgaa catcctgctg
      481 ttcttaatac catgaaatat ttagagacaa aggggtttga cgttacatat ctgcctgtag
      541 atgaaaaagg gaatctgtcg atcgaagagg tcaaaaaagc gttacgagaa gatacgatct
      601 tggtctctgt gatgtacggg aacaatgaaa tcggaaattt gatgccaatc aaagaaatcg
      661 gtgaactttt agcggatcat ccagcttatt tccatacaga tgcggtacaa gcgtacggaa
      721 accaaacgat ccatccagaa gaattaggaa tcgatttatt aagtatctct gctcataaaa
      781 tcaatgggcc aaaaggcgtc ggtttcttat ataaacgaga cggagtaaat atccctgcat
      841 tgcttttagg cggggagcag gaagaaaagc ggcgtgcagg tacagaaaat ttagcaggga
      901 tctggggtat ggcaaaagca gttgaactgc tgtcacctga agaacgtgaa gcacgtaaca
      961 acaaatataa aggattccag caattgattc ttgatacatt agaagcaaac gaaatccctt
     1021 atcaagtaaa tggtgattta acgaataaac tccctcatgt gttgaatctt cgcttaattg
     1081 gcgaagcaaa tgatttgcta ctgatgaagc ttgatcttaa tggggtcgct atttctactg
     1141 gttcagcttg tacagcagga aatatcgagc cttcacacgt attggaagca atgtatggag
     1201 aagatacacc gatactaaaa gaatccatcc gtatcagctt tggtttggga aataccgaag
     1261 acgacatcca gtatttcctt gatcaattag ttcgtgctgt caaaaaataa ctagtgaagt
     1321 cattcttata tgaacagatg tctgagagaa cgccttcatt ttttagggaa cttaactgaa
     1381 aaacctagta aaaatcagga taaactttta taataaagag aaactaattt tatcgaggtg
     1441 aaaatcatgg catttgaaac aacagcaacg gtactgggcg caccaattag ctatcgtttg
     1501 catcctgatg caaaaagata tacattgcgc gataatggat ttacagaaac aaacggaggc
     1561 aattatcaat tgatccgacc attagatgcc acgccgcaaa gcaaagaagg attcaaatta
     1621 aaaatcactg tatcaaaaga tattcaaaca ttgaaaatgt cgatcactac agcaaatgga
     1681 ttgaaagcag tcaatatttt caaagatcca aaacagaaaa tgagccaaga taaattttat
     1741 tttctgatgg atgggatgat tagccgtggg ttattcgaaa aagtcgaatg atctgtggat
     1801 ataaaaagac aactagtttt gaaacggcct attgttagat gactaacttt gtaaggacga
     1861 cgcaaattag ttgtcttttt tatttttaaa tttaacacat gttgttattt ttaatatttt
     1921 gctgatttat tgtttctaag taaagaaatt taaattgaaa cgattgttat ttagggaaaa
     1981 aggtattttt tgaaaaaaga atcccgtata atgattgttg tgcaaacgct atacaaaatg
     2041 aaaaagaaaa gagggatgtt tatggaaaat gaacagtcgg tcgttttaac gaattggaag
     2101 agaaattatt tgttcttttt atcgggacag tttttgtcag gaatcactag tatggtcgtc
     2161 caatatgcga tcatctggta tttgacaaga gaaaccggtt cggcgacaat tttgagtttt
     2221 gctactttac taggaatgat tcccatggtt ttactaagcc catttgtagg accgctagtg
     2281 gatcgctggg ataaaaaagc tcttttgatt gtcacggata ttattgtagc gattttcgcg
     2341 cttattttag cagtagtcgg aacgatttca gaatccttcc cgatttggct agtttttgtg
     2401 tctttgttta tgcgttcggt cgcacaaaca ttccaaatgc caacgatcca gtcgattatg
     2461 ccgacaatcg ttccatcttc tcacataaca aggacgaatg gacagttagg aatggtccaa
     2521 tcagcgaatt tcatcattgc gccagctcta ggtgcggctt tgttttcagt cgttcctgtc
     2581 aattacctta ttttattaga tgtattgggg gcagtttttg gtgtcggctt attgattttt
     2641 gtgaagatcc caaaagtttc tcctgaaata ttggaagttc cgcttactat ttttaaagat
     2701 gcaaaatttg ggctgcagca attgatggat aataaagggt tatggtacat aacgatcaat
     2761 ggtgcatttg tgatgctgtt gttcatgcca gcaatcagtc tgtatccatt gatgacgcta
     2821 gattactttg gtggttccgt tggacaggcg ggagcagtgg aagtagtcta cgcagtcgga
     2881 atgctcttag gcggtgcact gatcagtttt ataggaacat ggaaagaccg aatgaaacct
     2941 attattatag cctacatcat tatgggcctg acgatcggtg caagcggatt ggttccaaac
     3001 gatagtcaag ggttcttgta ctttcttatc ttgaatgcag gagcaggttg tgcaacgcca
     3061 tatttcaata cactgctgat ggcaatgatc cagcagagtt atgaatcgaa tgttttaggt
     3121 cgtgtcttag ggaacttcaa ctctttgatg aacctgacag gtccaattgg attattattt
     3181 gcaggaccat tagctgatcg tttaggggta gaaaaaatgt tcttgttttc cggaatcggg
     3241 attttattat gcggaatcgt tttattttta acgtcagctg cccgaaatta tgataaagaa
     3301 ttacagaaaa aactggtaaa agaacaccat gagcaaaaag acgaatagta tggaatggag
     3361 ttgtgacaga agtcttgtta caactccatt tctgatccta atgttcctgt ggcaataagc
     3421 ctactcaaat gaaaaatatg agtaattatc tttatttgtt taatacttga tatagggcag
     3481 ttttttcacg acctatcgta cttcctcttt gcttcataga tttctttgat ttgttgctca
     3541 gataacggag tgccaaatgg tagtttttcc tttaagagtt ctctttccgg tgcgtctacg
     3601 cctacattga catcgttctc gataggtacg ctgtaatgat ggatatgtcc atgaagattg
     3661 cggatagtgt cgttaggccc gagcaacatc ggataatgag tcaagtaata ttgttgatgg
     3721 tcgaatttaa cgatggcacc tacatcataa aaataaaatt tgggacttcc gtcagtaagc
     3781 ctaggatcat ttttttctaa ataacggaaa aaagcgcgac tgtcatggtt ccctttgatc
     3841 aagtggattg ttccttgcag ttgagtgacc agttccaaaa tttctgcatt tgctttttca
     3901 tattgcggat gcatagcaag gtcgcctaga tgataaacat ggtcggtttc ttttattact
     3961 gcattccagc tagtgatcaa ctgttggttc atttcatcca catttgcaaa gagccgcgga
     4021 gcaaagtcgt tttttcctaa taagcgttca tggaaaaagt gcatatcact gataaagtaa
     4081 ttcatgtatc ttttgcctcc cttttttctt agagtaaaca aaatcactta tctgagcaac
     4141 tcataacatc taaaaagaaa aggttgtaaa tgagacgtgc agctttaagc gataagaagc
     4201 aattttgaaa aatagttttt cacaatttat aaaattattc cttattgccg aaaggctggc
     4261 cctttttatg agtatgccaa ttttttatat cgaatgctca aaagatgagt gattttttgg
     4321 ttgactatca tgtttgtgta ctgaataggt ccagtagaac agtattttta cgacttgata
     4381 attaagggat gaaattttca gaaatatgac gtataatgta aaatactgaa aagttgcgac
     4441 cttcaactcg tagatgtttc agaatctaat tgaaatgatg gtgatatcat gacagacaac
     4501 agcaaaacac gcgttgtcgt agggatgagc ggcggtgtcg actcatctgt aacagcactt
     4561 ttgttaaagg aacaaggata cgacgtgatc ggaatattca tgaaaaattg ggatgacacg
     4621 gacgaaaacg gtgtatgcac ggccacggaa gattataagg atgtagcgaa agtagctgct
     4681 caaatcggga ttccttatta ctctgtgaat tttgaaaaag aatattggga tcgtgtattt
     4741 gaatatttcc tagccgaata ccgcgcagga cgtacaccta acccagacgt gatgtgcaac
     4801 aaagaaatca aattcaaagc attcttggat tatgcaatgg atcttggcgc cgattatgta
     4861 gctactggac attatgcgca agtcactcgc gatgaaaacg gcgtggtcca catgcttcgt
     4921 ggagtagaca ataataaaga tcaaacttac ttccttagcc aattatcaca agaacagctt
     4981 tcaaaaacga tgttccctct aggcggcatg gaaaaatcag aagtacgggc aattgctgaa
     5041 cgcgcaggcc ttgcgactgc taagaaaaaa gattcaacgg gaatctgctt tattggtgaa
     5101 aagaacttca aacaattttt gagcaactat ctgccagcaa aaaaaggaaa tatggtcact
     5161 cttgatggtg aagtcaaagg acaacatgct ggcttgatgt attatacgat tggtcaacgt
     5221 caaggactag ggatcggtgg cggaggagat tcgcaagaac cctggtttgt tgtaggaaaa
     5281 gatctagcga caaacacatt atatgtgggg caagggttcc atcatcctgc attgtatgcc
     5341 acaagcttgg atgcaagtga aatccacttt acgacgaatg aaccaatgcc aaaagaattc
     5401 aagtgtacag cgaaattccg ttatcgtcag caagacgttc ctgtgacagt tcgcttattg
     5461 gatgataatc gtgcagaagt cgtattcgac gaacctgtac gtgcaatcac tcctggacaa
     5521 gcagttgtat tttatgatgg aatggaatgt ctgggtggtg gtttgatcga ccacgcatat
     5581 caagaaacaa aagttttaca atacgtttaa gcgatattaa atgaatcaaa acagctatgg
     5641 ctaacaaaag aaacaccttt tcttttggat agcatagctg tttttttgca ataaaattaa
     5701 tttttattca ttttatactt gaaatacttg tgtaaaaagt tcataatatc caataatatg
     5761 aataaagttg ctttttgtta agcaagagca gttgcaaaat agaagggaat cttttgcaag
     5821 tgatttgttc aattttataa gtgagggtga tggaaatgga aaagaaatcg acgggtatta
     5881 gcaaatgggg actggttgct ctggttgtca gttcgtcaat cggaagcggt gtgtttggga
     5941 tcacaagtga tctggctagt tctgcagctc cagggccagc gatcctttca tggataatcg
     6001 ttgggattgg tattttagct ttggtattat cgctgaatca tttaggagaa aagcgtccag
     6061 atctagatgg tggtattttc ggttacgcgg aagcttcatt tggtaaatta ggtggcttca
     6121 tcagtggttg gggttactgg ttgtctgcgt ggctgggaaa tgttgctttt gcaacgatgc
     6181 tgatgagcgc catgggagaa tttttcccga tattcaaagg tggacaaaat gttccttcta
     6241 ttttatttgc aagtattttt atttggggtc tgactttatt agtaaacaat ggagttgaag
     6301 gagcgagcgt catcaataca atcgtgacga tctgtaaatt agtgccatta ttcctatttt
     6361 taatatttgt atttatcgca tttaaagtga atatattcac tgctgatttt tggggtaacg
     6421 tatctgataa tctagtgaat ggaacagggc agcaaggaac gatctggttg cagattaaag
     6481 gttgtttgat ggttatgatg tgggtctttg tagggatcga aggcgcaagt gttttagcca
     6541 accgcgcgaa gaaacgttca gaagcccagc aagcatcgat cattggtctg ctttgtttat
     6601 tgtttatcta tatccttgct tcgatcttgc catacggcgt cttatctcaa gaagagttag
     6661 caactatttc acaacctgct atggcaaata ttttaaaagg aatcgtggga aattggggag
     6721 cagcgctgat caatatcggc ttgattattt cgatcattgg ttcttggttg tcttggacga
     6781 tgctcccagc agaaacaacg atgttgatgg ctcgagacgg tctgttgccg aaaggttggg
     6841 gaaaaacgaa taaaaaaaat gccccaactt attcgttagt tgttactgca atcttgacta
     6901 atctattctt attgactttc ttagttactg attatgccta tcaatttgcc tattctttat
     6961 gtacagcggc aattttgatt tgttatctac ttgtcgggat ctatcaagtc cagttttctt
     7021 taaaagaagg aatcaaaaaa caagtagtga tcggtgggat tgctgtagtt tttgaattga
     7081 tgggcatcat tttagcagga ttttcttatg tccttttatg tagtattgcc tatcttcctg
     7141 gtttttgctt ctactggatc gcttgtcgtg aaaccaaaca tcaaattacg ggaaaagaaa
     7201 aagtaatgat tgggctgatt tgtgtggccg cactgctttc aatcgggttg cttgctgtag
     7261 gctggatcaa gatataaggg ggaaagaaac atgaaaatcg caaatttcgg tgtagaagaa
     7321 tggctaaata aatgggaaaa gaaagcggta tatgatattg cacaaagctc gatcgaagca
     7381 ttgacattag acgaactcgt tggcttagat gggactagcg taagtaaatt tttcgaaaaa
     7441 cgcaaaaacg aaccattgga ctatggctgg atcgaaggat cagatgtttt caaacaagaa
     7501 gtcgcttctc tttatcaaac cgtcgatccg gaaaacatct tacaaacgaa tggagcgact
     7561 ggtgctaatc ttttagctct atacgcgtta gtggaagcag gagaccatgt tgtttcgatg
     7621 cttcctactt atcagcaatt atatgacatc cccaaatctt taggtgccgc tgttgacttt
     7681 gttcatttga aggaagaaga agattggcag tttgatttag aacagctaga agccttagtc
     7741 aaacctgaaa caaaaatcat ctgtttgaat agtgcaaaca atcctacagg cacattgcta
     7801 gatcggaaaa ctttagaaaa aatcgcagaa attgctcgta cagtagatgc atatgtattg
     7861 atagatgaag tatatgcgcc tttaacggat aaaggtgaat tcttgtcgat cgtagatgtc
     7921 tatgaaaaag gtatagcaac caattcccta tccaaaacct actccattcc aggtattcgt
     7981 attggctgga cagctactgg acctgagttg gcagaagttt ttcgaaagta tcgagactac
     8041 acgatgattt gcggcggagt cctgtcagat gatcttgctg tacatgcatt gaagaataaa
     8101 gaaaaaatat tggcgcgaaa caaaaagatc attacagaga accttgtgat tttaaaacaa
     8161 tgggtagcca atgaaccgaa agttgaactt gtcgctccca attacgtctc tacttctttc
     8221 attaaattag ccatcgaaga agatgaccag acattttgta tcaatttatt agaagaaaca
     8281 ggagtactat tagtaccagg aagtgccttt gatttaccga agcatgcccg actaggttac
     8341 tgttgtaaaa aagaaacctt ggaaaaggga ttattcctct tatctgagtt cttgaaaaaa
     8401 caggcctaaa caaaaacact gcaaggacag tctgtgacaa ctgttcaagc agtgtttttc
     8461 atttacgctt attgtctatg agaaggagag tgccctgaag ctatggagac tcctgaagct
     8521 ttttcttctg cagtttcttg ctgatggatc tcttgtgtat tgtgtcgtac aagcaagaag
     8581 ttgaccaaaa caagaagaga agtcgaaaga aagacgccac gataaccaaa tgcactggaa
     8641 acactggagc cgatcatagg ccctactacg ttaccagttg cttggaaaga ttggttataa
     8701 ctgaagatcc gtccagcagc ttgatgaggc gaatattttg tgatcaatgc ttggacagca
     8761 gggagcagac aagcatcaga gataccaatc aaaaaacgta aggcagctaa ttgccaaaca
     8821 ttcgtgacga atgccattgg gatataaacc agaatcgcaa aacccaaacc aatcgcaagt
     8881 attctttcac tcccaatctt gtcacctaag cgaccaaatc ttggagcggc tatcaaagtg
     8941 gcaatcccag gtatcgaagc aatgattccg ctgatcaacg taacattccc atgtccatgc
     9001 agcaattggc gaatgtacag actgatgatt ggactaatgg aattgttgga tgcttggatg
     9061 atcatcgttg tgacgaacat accgatgacg acgtgtggat atttcaagtc tttgaaaatc
     9121 tgtttggcag aaaccatgtt ttcttttttc actgggacaa atttttcatg gacgaaaaac
     9181 agactcaaca agaagacgaa aaataagatg atccctgtaa tgaaaaaggt agggcgatag
     9241 ccaaacgctg aagcagagat cccgccaaga agcggtccaa gtaatgttcc tgtaacagaa
     9301 ccagttgcta aggttccaag aacttgacca cttttttctt ttggcgtccc agttgcgaca
     9361 agggcagtgg cattactgat ataaccagaa aaaatccctt gaagcagacg caatgcgact
     9421 aattgataca cactagtcac gcaacccatc aaagaaatga cgattgccat tcctaatgaa
     9481 gcacgcaaca gcatcaattt tcttcctttt tggtcggcta attttcccca ccaaggagag
     9541 ataagcgtag tcaccaagaa agtagatgaa aaagtcaatc cgctccaaaa gttcaattgt
     9601 gacgtactat aattacctaa cgtatctatg taaagtgaca taaaaggcat gactaaacta
     9661 aatccgatac cagccataaa tgttccaaac cataagacaa acaagttttg ttcccaagtt
     9721 tctcttttcc taaataattt ttcctttatt cttgcagcca attagactcc ttctttctga
     9781 aaattttcat ctacagctct ttattataaa ctacaatatg tagtttatca atgaaagtct
     9841 cttttttctc tttctttttc gtggtaaact gaaagagtga aggatttatt ttatgagagg
     9901 aaacatggaa atgaaaaaaa gaaatctgtc tcaagagaaa atcatcgact gctttcgaga
     9961 actagcagaa gagatggatg ttcagcaaat aacctttcag catttagcca aacgcttgga
    10021 tatcaaggct ccctcgctgt ataaccattt caaaaatatt cgtgatgtca aaacagcgct
    10081 aacagcgaaa cttctgcagg aacttaatga ccagctgcga cgggctcttg tgggaaaaag
    10141 tggtcaggaa gcactgcaga tctatgcaga aacttatcga tcctttgctt tttcaaatca
    10201 agcagtgtat gagttactga tcagtgtacc gcataccaat gaagctgttt tattagatgg
    10261 aatccatgaa acgaatcaga tcattttgca gatatttgag gcttttcaat taacgaaaga
    10321 agaaaaagtt catcgaagca gggaacttcg tagtatgatc cacggttatc tttccttacg
    10381 ttttttagga tactttacaa aagaaccaac aatcgatcca gaagacagct atttttggat
    10441 gatcaataac tttatcgcta ctttgcctta aacaatcaga ttcatttgta acaattttaa
    10501 gatgttcatt aaatgaccct tttataagag aaaattcttg caactttctt gaaaagtgac
    10561 taagatgagt ctagaaaagc tgaaagaagg agcacgagta tgtatcaagt aattaccatg
    10621 ttcggggaca atgagccttg gtggtttttt gaagactggg aagaagatat tgaagaagaa
    10681 gaaacattcg agtctttcga agaagcacga caagcgtacg aaaaacaatg gaacgaaatc
    10741 catcaaaatt atgaatatat caatgcaaaa agtaactttc tcagcgcctt ttggaatgac
    10801 ggagatgagc gctggtgtga agagtgtgac gatgatttac agcaatacaa gggacttgcg
    10861 ttgttgaaaa atcatcagcc catcacagta gaaagcagaa aggaatttta tgaaacaact
    10921 aattctagcg gaaaaaccaa gcgttgcgaa agacctaagc aaggtgcttg gtgctaatca
    10981 aaagcacaaa aattattatg aaggacccaa ggtgatcgtt acttgggccc ttggtcattt
    11041 attaggattg aaaatgccgg aagatctgaa taaggaatgg cagagctggc aaatggacac
    11101 gttgccaatg atccctaaaa aactaggaat caaaccactt cctaaaacag gacatcaatt
    11161 aaaagcaatc aagcagctgg ctcagcgaaa agatgtctca gaagcagtca ttgcgacaga
    11221 tgccggaaga gaaggggaac ttgttgctcg ctggatctta gagtatgtgc atttcaataa
    11281 accagtgaag cggttatgga tctcttctca aacagataaa gcaatcaaaa ctggattcaa
    11341 gcaactaaga cctgcgaaag aatatgatgc attgtatgaa tctgctcttg cccgggcaaa
    11401 agcggactgg ctggtaggat tgaatgtgac cagagcctta actgtgaagt acgcggataa
    11461 tttatcagca ggccgtgtgc agacaccgac actagcatta gtgagagacc aagaaaagaa
    11521 aatcgagcag ttccgtccac aaacgtattt cacgatcacg ttgcaagctg gaaaagaaca
    11581 agccaaaatg cttcaaaaaa atcaatttgc tttaaaaaca caagaagaag cacaaaaact
    11641 tgttcaattg atcagtacaa gcaaaggaac tgtcacagaa gtccaagaaa aattaaaaac
    11701 ggaatctgcg ccattgcctt atgatttaac agagatccaa agagaagcca atcaacgcta
    11761 tggctattct gcaaagaaaa cattaagtct tgtacaaagt ttgtatgaga cccataagat
    11821 cgttacttat ccgcgtacag acagcaaata tctgacaaat gacatgaaaa gtacgatgaa
    11881 agaacgattg caagcagtca gcgactttgc tcctgaggtc aagcagtatt tgaaaaacgg
    11941 agccatcgtc caacagacaa aagtattcca agacagtaaa gtgaccgatc accatgcact
    12001 tttgccaact gaaaacagag cacgttatga aaaattaagt aatgaagagc gaaaaatcta
    12061 tcaaatgatt gtctctcgtt ttttaggact gtttgctcaa ccccataaag tcagccaaac
    12121 aaaagttacg gttgaatttg gtaaagaacg attcattttt cggcaaaatc gtgtcgtcca
    12181 agcaggatgg aaaggcgaaa ctgaaactga gacggaaaca gtcaaatggg aaaaaggaat
    12241 gagaatcaat cccgatttcg ccatcaaaaa agaactatct gcaccgccta aaccattgtc
    12301 agaagcaagc ttgcttggtc aaatggaaaa atacagtctg ggaaccccag ctacgcgtgc
    12361 agagattatc gagaaactaa tcaaatctga actgatggaa cgtacccctc aaggactgca
    12421 agtatctcct aaaggtaagc aattattagt attggtaaat ccctctttag taacaccaga
    12481 tttgacagaa agctgggaaa aagaccttga agctattgct gcaggaaaga aacaagcagg
    12541 aaccttcttg aatggaatcg aaaaagaaac aaaacgcttg gtgaatgaga tcaaatcaag
    12601 caaacaagaa tatcaagatt tctcgattac ccaaaagaaa tgcccagaat gtggggccaa
    12661 tctgcgcgag aaaaacacac gtgacggcaa aatctatgtc tgcaccaatt cagattgcaa
    12721 ctaccgcaga agaaaagatc caaaagtctc taatcatcga tgcccgcaat gtcaccggaa
    12781 aatggaaatc atcgaaggta aaaacggtgc ttatttccgt tgtaaatacg atggaacgac
    12841 agaaaaattg cttgataaaa aagagcagaa gaagaaaatg accaaacatg aagaacgacg
    12901 cttgctgaaa aaatatgcac aagcagaaga accagaagaa agcccattag cagcagcact
    12961 aaaagcagca atgaagaagt aataagatat gaaagaggtt gtgatagaag tggtcagctt
    13021 caagaaataa gaaggaattt acgaaaattg cttctcaaat ttttgtgaaa tttcggctta
    13081 tttccgaaga agctacttct ggaacaccat ttattcggaa aaaaggactg tgacaagact
    13141 tttgtcacag tcctttttca tttaaaaaaa tgtttacttc atttgacgta tattattggt
    13201 gaggcttcaa ttcatttggc ttcacttttt ctgtttctct tatgatataa acaggacgct
    13261 gtttcgtttc taagaagatt ttcccaatat atttcccgat gattccaaga cataacaatt
    13321 ggattccacc aatgaacagg aagatcgaga ccatggaagg ccatcctgat gtaggatcac
    13381 caaacaacaa tgcccgcaaa ataatgaaca aaattgcaat accagaaccg acacaagaca
    13441 aagcgccgat aaatgaagca atcgttaacg gtgcatccga gaagttcacg ataccatcaa
    13501 tcgagtagtt gaataatttc cagaaggacc aagatgtttc gccagccaca cgttcgcggt
    13561 tttcataaga aatatattct gttttaaatc cgacccagct aaataatcct tttgaaaatc
    13621 ggttgtattc tgttaattct aggacggcat ccaccatttg acgagtcatc aagcggaaat
    13681 cacgagcgcc atcgaccatt tctgtatcgc tgattttatt gatcaactgg tagaattttt
    13741 tcgcaaagaa actgcgtatc gcaggctcgc cttcacgagt cgaacgccgg gtgccgacac
    13801 aatcgatatc agaagtagtg atccggttga tcatctcagg aagcagttcg ggcggatctt
    13861 gcagatcaac atccatcacg gtcactaaat ctcctgtaga agcttgcaat ccagcgtaca
    13921 gagccgcttc tttcccaaaa tttctggaaa atgagacaaa gcggacataa ggataagctg
    13981 aggctagctt cttgattgct gctaacgtac cgtcttttga tccgtcgtca ataaacaaat
    14041 attcgaactc atgggcacct ttgaattttt caacctcatc aaaaaaaaga ggaacagttt
    14101 tttcttcatt aaaacatggg acaacaattg atactttcat cgtttctatc ctttcaatcg
    14161 tatgtttata tatggtttac attcaaaatt taataaacct taaataacac ctttaaaatt
    14221 tgattttacg taaaagaaca ggtattcttt ttgtaaacta acgtatttag tttaccatgt
    14281 ttcctcggtt tatcaaactt ttaagaacat tttttattag ttggcaagta gttttggata
    14341 gagaaaaagg aggaagttcc agtctattga ctaggagcaa acaaaatgaa aatacacaca
    14401 cctatcggct ttttatggtt agatgcagat gaatccggat tgactgctgt atcttttcag
    14461 ccaatttcag aaagcgaatc agcgaatcag cgaattttaa cgcgcacaca agaacagttt
    14521 gaagaatatt ttgcgggaaa aagaaaagaa tttgatgttc ctttatctat aaagagaggt
    14581 acgccttttc agcaaaatgt ctggaaagct ttgtcgacca ttccttatgg agaaatccga
    14641 acgtataaag aagtagccgt tgctgttgat agtccgaaag ctgtgcgagc gattgggcag
    14701 gcaaatcgaa ccaatccatt gccaatcgtt attccttgtc atcgagtgat cgggcaaaat
    14761 ggaaaactga caggatatat gggaaacgca atagatgggg ttgaaatcaa acgccaattg
    14821 ttgttgttag aaggatatct gcaaaattaa aaacagttgt gattcggaca ttgttccatg
    14881 cgagaatcac aactgttttt tcatgttcat tctaaacttt gatcagccaa ataaatagta
    14941 aagccatcac tgtttggaga agcccgacag ctaatccata agataagaag cgtgtacctt
    15001 ctttcatgaa ttttgcaaag tctaaacgca gaccaattgc cgctaaagca gtgatctcaa
    15061 accagctgct gataaaatgt gccgattggt caataactac aggtaaagaa aacaagctgt
    15121 tgaccaaaca aaagatcaca aaccctaata cataccaagg aagcggtaat tttcgttttt
    15181 tctgcgtatc agttggcgtt tcaaacagag ctggttgttt cttgtgcatg aatttttcaa
    15241 acagatagac aacaacaacg agcatcataa tacgtagaat cttaaatagc atggcgaatt
    15301 cgacaacaga attgtcaagt aagcttgccc cagcgactac ttgaccaaca gattggagtg
    15361 taccacccaa aagtgcactt ttttcaatga gatttccacc aaacaactgt aatccaagaa
    15421 aagggagagt caacatcatc acagtaccca gtagattgac tagtgtgatg atctgtcctt
    15481 tttcttcctc tttcgcatgg atggaagggg cgattgaagc aatagcagaa gaaccacaaa
    15541 cagcgtttcc tccagccatc atcaaagaag aagtatcgct gaattgcagt tttttaccaa
    15601 tcaagtaagc accgatgata gtgactgaca tttgcagtaa cacaaaaaca attcctttta
    15661 caccaatttg agcaatcgtt tgagctgtga cagttgcacc tagtaaaaaa acagagaatt
    15721 ctaatagttt gctttcagca aattttgtgc cgattgctag aacaggctgt ttaaaaaaag
    15781 tatttcctaa taaaataccc aataaaatag caatagtcgc tccacctaac tgtggcagat
    15841 aaatagcaag aagcctgctg aaggcagcga tggcaaaaga aagcagtaaa ccgggtaaga
    15901 tttcgcggac cgctgaatcc tttacagatt ttattggatg tgacaatgat ttcatttgat
    15961 cggttccttt cgtcaataaa tactaaaagc atactcgaac atagtagcac taaaccagta
    16021 ttttgacaaa agaacaagag gtcgtgacaa aagtcttgta cgacctcttg tttcgaata
//
LOCUS       DAYYCY010000053        16079 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000053 DAYYCY010000000
VERSION     DAYYCY010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16079
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.053"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1405
                     /locus_tag="ACHAZB_001339"
     CDS             <1..1405
                     /locus_tag="ACHAZB_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFM8614024.1"
                     /translation="NILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLNDINNIKKE
                     FEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDEPDYLQLTE
                     CEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRLGGFNSWEL
                     PESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTIDSKFIEEIK
                     KRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDFQQLHQVFI
                     DNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKFYLLNEEQT
                     NLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYIVTNSDVHY
                     LIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDAVQYENIIP
                     ISINTIDRAIISAVQNK"
     gene            complement(1497..1862)
                     /locus_tag="ACHAZB_001340"
     CDS             complement(1497..1862)
                     /locus_tag="ACHAZB_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFM8614025.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPLDVQNK"
     gene            complement(1859..2224)
                     /locus_tag="ACHAZB_001341"
     CDS             complement(1859..2224)
                     /locus_tag="ACHAZB_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFM8614026.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2416..2664)
                     /locus_tag="ACHAZB_001342"
     CDS             complement(2416..2664)
                     /locus_tag="ACHAZB_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8614027.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2663..2791
                     /locus_tag="ACHAZB_001343"
     CDS             2663..2791
                     /locus_tag="ACHAZB_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HFM8614028.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2848..4542
                     /locus_tag="ACHAZB_001344"
     CDS             2848..4542
                     /locus_tag="ACHAZB_001344"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HFM8614029.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4642..4815
                     /locus_tag="ACHAZB_001345"
     CDS             4642..4815
                     /locus_tag="ACHAZB_001345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8614030.1"
                     /translation="MQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQA
                     FEHRLPRGYEKGV"
     gene            5193..6326
                     /locus_tag="ACHAZB_001346"
     CDS             5193..6326
                     /locus_tag="ACHAZB_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFM8614031.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6357..6677
                     /locus_tag="ACHAZB_001347"
     CDS             6357..6677
                     /locus_tag="ACHAZB_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HFM8614032.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6706..7170)
                     /locus_tag="ACHAZB_001348"
     CDS             complement(6706..7170)
                     /locus_tag="ACHAZB_001348"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8614033.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7302..8555)
                     /locus_tag="ACHAZB_001349"
     CDS             complement(7302..8555)
                     /locus_tag="ACHAZB_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM8614034.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8584..8901)
                     /locus_tag="ACHAZB_001350"
     CDS             complement(8584..8901)
                     /locus_tag="ACHAZB_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFM8614035.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIINLYRR
                     LNA"
     gene            complement(8927..9247)
                     /locus_tag="ACHAZB_001351"
     CDS             complement(8927..9247)
                     /locus_tag="ACHAZB_001351"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8614036.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9711..11126)
                     /locus_tag="ACHAZB_001352"
     CDS             complement(9711..11126)
                     /locus_tag="ACHAZB_001352"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8614037.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11143..12459)
                     /locus_tag="ACHAZB_001353"
     CDS             complement(11143..12459)
                     /locus_tag="ACHAZB_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8614038.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12609..13307)
                     /locus_tag="ACHAZB_001354"
     CDS             complement(12609..13307)
                     /locus_tag="ACHAZB_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFM8614039.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(13596..14585)
                     /locus_tag="ACHAZB_001355"
     CDS             complement(13596..14585)
                     /locus_tag="ACHAZB_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFM8614040.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(14665..15498)
                     /gene="msrA"
                     /locus_tag="ACHAZB_001356"
     CDS             complement(14665..15498)
                     /gene="msrA"
                     /locus_tag="ACHAZB_001356"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFM8614041.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(15673..>16079)
                     /locus_tag="ACHAZB_001357"
     CDS             complement(15673..>16079)
                     /locus_tag="ACHAZB_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8614042.1"
                     /translation="AYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNLPYKT
                     TGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGVADIK
                     VSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
BASE COUNT         4996 a         3252 c         2735 g         5096 t
ORIGIN      
        1 aaatatttta agacagctgt ttttatgtgg gcaattctat gtaaataaag aagtcaatct
       61 agaaaagctc tccaatctcc tgcatgtttg caaaaccact ttactcaatg atatcaataa
      121 cataaaaaaa gaatttgaag aacagatcgc ttatacacat cgggaaaagg accgttacac
      181 tttatatttt tcagaacata tacctcgatg caagatcatg cagcaattgt ctcaaaattc
      241 gttgttttta aaaacttgtc tgctttattt ggaagaagat gaacctgatt atcttcaatt
      301 aactgaatgt gaattcatct ctgtcagcaa agcttattca ttaaaaaaac aagtattagc
      361 ttactttaat gactgcggga tcgaaatcga ccgctactcc cctcgattca ctgaaatgga
      421 aagacgtctg ctgcttctta atgtgtcgta tcgtttaggc ggttttaatt cttgggagct
      481 acctgaatcg ttttttgaac gtgccgatcg tttcattgaa tccgtaacgg aaaatagtgg
      541 cagattttat gataaagaaa ataaagaaat cttatcgatt ggttttgcta tcagtttcct
      601 acgccagcaa gtgtgtgctg tgacaataga ttcaaaattt atcgaagaaa tcaaaaaacg
      661 tcctatttac aattatgtcg aatcagcatg ggaaaacact gatttccaaa cctattataa
      721 aaaagaagaa ttcgccttta ttttgacact attcaatctt tgtaattatg gtttccattc
      781 ttatcagttg attgcggaag actttcaaca gctacaccaa gtgtttattg acaatactcc
      841 cgaaataaaa gaactagttg ctacctttga atctcacttc aatcaagaat tattcggaaa
      901 ccaaccgttc gaacgtgcgc tgattcatct gatgcgttcg gcttgggata actatcaatt
      961 attcatgcca gaaaaatttt atcttttaaa tgaggaacag acaaatttat ataaagaagt
     1021 ccagacaatt ttttccagct ggtccagcca gttaccttat gatctacggt tgaatcctaa
     1081 ttgcatgcgg gcttttgtga tcgaattgag cggaattctc cgtctgacaa aagaacatct
     1141 gactatctat atcgtcacaa attcggatgt tcattatctg atttatcggg aagccttgga
     1201 agctgtaact actttcgatt ttcaagttgc accaacaatt tattcaagta tatctgatat
     1261 aaagaagtat gcgcagcagt cctcaaaccg ggtattatgt gaacgtacat tatatacacc
     1321 agatgcggtc caatatgaaa atattattcc tatttcgatc aatacgatcg atcgagctat
     1381 catttcagct gtgcaaaaca aataaggggc tgtgacaaaa atcatgtcac agcctcttat
     1441 ttgttgaaga tgcccatttc tgtaacaatc tcttgataca cgtcaggctc acaacctcac
     1501 ttattttgga catctagggg tccgatctct ttatttaaaa aataagaaat gatggttcgg
     1561 ataactacca acgccgctag ttggaaaata tcctgcaagg taggtttgat aatcgattcg
     1621 atgatcccag ctgaaacaag aatttccaaa cttagaagta tatagcctcc caacatcttt
     1681 ttaatagcat tgttctcgct tactcggtcg ctttgcactt ttgtatggac aaataaatat
     1741 ttcaaaaaat ccttgattga tagacaaaca ccccaaacca ataaacagat agcaaagata
     1801 tcaagtccca gtacaagttt ttcgaaatat ggaagaagaa actgcatcaa ctctgtcgtc
     1861 atcctacctc tcctcttctt tgtttttctt tgtcttctat ttcctgattc aagaaataag
     1921 aaatgactgt tcgaatgata ataataagtg caagcttcaa aatatctgtc caagtcggct
     1981 tgataactga ctcgatgata tcagcaacga ctagtacttc caagcttaac aaaatataac
     2041 ttgctagcag ttttcttgta tctgcattac gagtattacg ttctgcatag tttttcttga
     2101 catgagacca atcaaaaagt ttgaatagaa caagaacgat cccgacaatt aagataccga
     2161 tagaacaaat atttaaaata gcaatgatcc attctagaaa ttcaattaaa tttgagcgaa
     2221 acatatacgc tacctacttt cttttaattt gttcctattc ttttattgta aaagaaagtt
     2281 accttgttcg tctatttaaa agttgttcta atgataacgg ttcgcttaaa tttttctttt
     2341 tcactctccc atttttctcc ctccatccat aaatcgtgga agtatccaaa acaacgtagc
     2401 aagttaaaat aaggcttagt ccgtcatcag cttttcaaag aagcactgtc tcggtgcttt
     2461 ttttgtaccc aaatttctaa aaatagaacc atacacctcc tcaaatcttt tcttcttacc
     2521 taccctattt ttatttacag gaaaatcaga cacttcttct tttttgtcaa aatttccttt
     2581 ttcttctcgt cctatcattt ggcattctaa taaaaacgaa ctatccttgg atatagaaag
     2641 agaaaacaag gaggcgtttt tcatgagtaa aaaattaatc aaagtcggca tcggtttagg
     2701 tttgcttgcc cttggcgctg catatctagg aaaaaagaca ggactattcg aagatgacag
     2761 tcatttatat gatgaattcg aatcaatcta gcatcctatt tagttgaatc actacggaca
     2821 tacttggaag acattggagg aatcacaatg agaaaaagta aagcaataat gattggtgcc
     2881 ggtattgcca atatggcagc ggctgtttat ttgattcaag aaggcaaatg gaaaggggat
     2941 cagatcacct tttattcatt ggatgatcac ggttcaaatg atggtgcacc gaccgacact
     3001 gtagtggatg aatattggaa taaaaaccat ccattagaaa accaaaaagg ctatattgct
     3061 cgaggcggtc gcatgcttaa ttaccgtacc tatgtagatt tgatggatct gttaagtcga
     3121 ataccttctg ttactgagcc agggatgact gctgaagaag atacgagaga ttttgatgca
     3181 aaacaccgaa cctttgacaa agcacgtttg atggaaggtg gcaaaggaat cattgatggc
     3241 ggaaaactgg gattcaataa taaagaccgt attcttctga ctaaactgat tgctatgcct
     3301 gattcagaag aagaaaaact agacaatatc actatcgaag aatatttcaa agatgatcca
     3361 cacttttttg aaacaaactt ctggtttatg tgggaaacaa catttgcttt cagaacacgt
     3421 agttctgctc aagaattgcg tcgctatatg catcaaatga tttatgaatt cactcaaatc
     3481 gaacatctag tgggcgtcaa tcgaacacgt tacaatcaat acgaaagtat tatgcttcct
     3541 ttgatcgagt acttgaaaga acaaggatgc aaaatcattt tgaaccgccg tgtcactgac
     3601 tgggaattca aagaaacacc gatgcaagat gaaatcactg tgactggttt gaaaattacc
     3661 aatacactga ctcaagaaga agaatacgtt gtggtcgatg aagatacagc agttattttc
     3721 actaatgggt ccattacaga ttcagctact ttaggagatt acgaaacacc agctgtagaa
     3781 aatatggatt atggtgcagc ttctagtctc tggaaaaaag ccagtgaacg tttctacaac
     3841 ttgggaaatc ctgacaaatt ctttgctgat cgagatgcaa gcgaatgggt cagctttacc
     3901 ctgacgacga aaaaccattt gttgttgaat gaaatcaccc gtatcacgac tcaagaacca
     3961 ggaaatgcct tgaattcatt tatttcaacc acaccgatca cgccgttagg ccaaaaagat
     4021 gtcaatatgt ctattgtcgt gcatcatcaa ccgcatttta cgtctcagaa accaaatgaa
     4081 acagttattt ggggttattt cctttatcca agacgacgtg gcgaatttgt ggataaacag
     4141 tatattaaaa tgaatggaaa agaaatgcta gaagaactga ttggacaact ttcaaaagta
     4201 gatcctggac cagtgaatat tcgtgaaaaa gaaacagaaa ttttcgacag cgtaataaat
     4261 aatatccctg tctatatgcc ttatgcttcc gcgttgttca ataatcgtgc caaaagcgat
     4321 cgaccaaaag tcattcctaa acattcgacc aatctggcct ttacaggaga atttgtcgag
     4381 cagccttatc agatgatctt tactgaacaa agtgctgtac gttctggaga aatcgctgct
     4441 ttccattttg cgggtattcc tatgtcccgt cttgtcaaaa caccgcgtta cgacaaagat
     4501 attcctactc tagctcgtgc cgcaaaaaaa atgtttgaat aatcaagagg ttgtgaaaaa
     4561 gctgccctgc atcaagtaat aagaacaacc aggctaaaac agcttcttat gtttttgatt
     4621 tggttggact tattaccgca gatgcaagct tttgaacacc gtttaccaag aggttatgaa
     4681 aaagctgccc tgcatcaagt aataagagca gccaagctaa aacagcttct tatgtttttg
     4741 acttggttag acttattacc gcagatgcaa gcttttgaac accgtttacc aagaggttat
     4801 gaaaaaggcg tttagtttcg agtatgaaga aaaattttgt gaaaatggtt cctcacgttt
     4861 ttgcataaat ttgacttaat accgagaaac tgatcccttc tgtcataacc tctttttatt
     4921 tatgtctgtt tctttttgaa aacaactatt ttttcaacgt ttactctctt gccaatccaa
     4981 catcatctaa cggatacaac tatttcaatg attaccatta cactaaaaaa tcggaaacac
     5041 acctctccga acacaaatac ataagactat agaatgtttc atttaagaaa aaaagcgttt
     5101 cattatcaaa aaggtaagat ctcctttaaa atgaaattat ctgtagtact aataatgcag
     5161 acaattttat gaatctgaaa ggagcatctc caatgaaagc agtcacttgg caaggaaaac
     5221 agaaaatgga aatcagaaac gtagatgatc caaaaatatt agaaccaaca gatgcaatca
     5281 tccgtatcac agcaacagcc atttgcggct ccgatctgca tctttaccat catggtgaag
     5341 cagttttgga aaaagggtat gttgtaggac acgaaccaat gggaatcgtt gaagaagtgg
     5401 gatctcaagt tcaaacattg aaaaaagggc aacgtgtggt tatccctttt aatattagtt
     5461 gtggtcattg tcatttctgc ctgaatcaca tggaaagcca atgtgatgaa tcaaatccag
     5521 atcagttata tggcgggctt ttcggatttg gcaagttgaa cggtaatcat tggggtggtc
     5581 aagcggaata tctaagagtt ccttatgcag actctacttc atttgttgta ccagataatg
     5641 atcttcctga tgaaaaagtt ttatttctat cagatatttt gcccacagct tattggagcg
     5701 ttgaaaacgc aggagtaaaa aaaggcgata cagttgtgat tctaggttct ggtccagtcg
     5761 gtctattcgc tcaaaaattc gctgttatgg ctggagcaga gcgcgtaatc gcagtagatc
     5821 cagtacagca ccgtctagat aaagcggcta attacaatgg ggttgaaact tatttcttag
     5881 aagacacggc acaagctgga acagaattgt atgaactggc taaaggtggc gcagatgtca
     5941 ttatcgattg tgtaggaatg gacggattag agcctgtcaa agaaaaagcg aaaaatttag
     6001 taagcttaca aagcggcaca atctctccat tacagatggc atcgcaagct gtcaaaaaat
     6061 ttggaacagt ccaagtaact ggtgtttata tgactccagc ttcttcttac ccgctgcaag
     6121 aattttttat gcgggatatc gctgtcaagc atggacaggc accaattatc catctaatgc
     6181 caaaaattta cgcaatgatt gcagaaggac tattcgatcc tacacaaatc attactcatt
     6241 cgctgccact atcagaagca gcacaagcat atgagatctt tgacaaaaaa gaagataaaa
     6301 atataaaagt tgttttgaaa ccttgaattc agtaaaaaat acaggtgatt ttctttatgc
     6361 ccaaaatcaa acgaaatacc catgagatcg aaataggaaa agaagaagat atcgaaatac
     6421 gaggacgtcg cttccgcttc tatttccaaa atcgttacac attgatctca ctggctgtcg
     6481 atttattaac cgggattttc tatatttttg gaagccttgc ttccttaact aaaatccccg
     6541 atcgatatgg cacctatttt tatttggctg gtgctatttt cttgacgatt cgtcctattc
     6601 tcagaatcgt aaggaacatt tttatttacg acgaaaaaaa agcaatgaag agaacaagca
     6661 aaaaaaaaga ggcatgagaa tttttcaaat ctcatgcctc ttttttcacc ctatcgtatc
     6721 actttgatac agacgagcgg tcgtcccact ttcttccttg accattgtca aaaatgtcca
     6781 ctcgtatcgt tcataaaacg tggtatgatc tgtaattaag taaagttttt cgtacccaag
     6841 ttgatgcatt tcttttctga tttccgctaa tatccgcttg gctatcccct gcttacgctt
     6901 ttccggttca acaaacaggg cacaaagatt tggtgtcaga tcagggcggt tatggaagtc
     6961 gttttcaatg acgcccgctc ctccgacgat ggtttctttt tcatctaaga ccacatacca
     7021 ttgaggaata ctttccttat tagcgattcc agtacgaata ctttcctcat acacaacagc
     7081 aggaactccc cattttgatg aaaaccaatt cgcgctttgt tttactaatt ctgggtgttc
     7141 tcttaatttg atccagttat atgtaaccaa tgtttaaacc tccgactaaa ataaaacgtt
     7201 tacaaaaatt atacaagtct ttatgaaaat tagcaatgac aacaatagtg atgtaaaaaa
     7261 aaaaacggaa aacaatcact tgttctccgt tttttgatta atcaagcatc taaagcttct
     7321 tttcttcttt cttggatcgt agctaccttg acgaacggta gatagatcaa aatagaaaga
     7381 accaaattga cagcggctag tacaccacct gcaatacttt ttgttgccaa tacaccgcca
     7441 ataattggtg gagttaccca tggaggcata acggtagctg ctggcactaa acctgtagaa
     7501 gtcgcaaagt aagcaacact aactaagacc attggtgtca aaataaatgg aatgaacatg
     7561 atcgggttca acacgatagg taacccaaac atcgttggtt cattaatatt gaagacacct
     7621 ggcgcaatcg acaagtttgt tacaaccata tatggtttgt ttttacgtcc tactaaataa
     7681 atagctaaca gtaaacctaa agttgcacct gttcctccta agttgacaaa gctgtcaaag
     7741 aaaggtttgt tcacgataaa cggtgcgcta tgtccagctg cgattgcatt aacgtttgct
     7801 tcaatggctg gcgcattgat tgtttgcatc aaaggatcga ccatattcgc tccgtgtaaa
     7861 ccaaagaacc ataagaatgg tgtgataaat gcaagcagta aggcagatgg ataagagttt
     7921 gcaagtccca taaatggttc ttggaccact gtatagaaag aacctacgat atcatcaaca
     7981 ccgaatgcag caaaaattgc agcaactaaa gcaaacgcag agatagtgat cattgctggt
     8041 agtaatgaag caaatgcttt tgcaactgcc ggtggcacac catcaggcat tttgatgatc
     8101 aatttttcat ttccgcttaa acgtacaaag atttctgttg aaataatccc aacaataata
     8161 gcaataaaca aaccgtttga gctcatacct aatgctccgc ctaaagcgaa gaaagagcct
     8221 aaagaaatca cacctgcgga aacaccgtct tttcggtagc cgttagctaa atgataagca
     8281 agcaagaaag caatcaagac agaaagaatg gcaaatgtac cgttccaagc agcagccccg
     8341 aatcctttcc atgattcacc accaaaaatt gagttcatca attcttggaa cccgggaatc
     8401 ggtaagttat tgatcattac tgctaatgct cctaagatca ttaacggcat actcaccata
     8461 aatgagtccc gaatcgcaac taaatgccgt tgtgcaccaa tttttgatgc tacaggtata
     8521 aaatgtttct ccataaatgc aacaaattta tccataaatc ttccctcttt tctttactgt
     8581 agattacgcg ttcaatcgac gatacagatt aatgatctct tttgctaaat ctgtaaaagc
     8641 tatagatgtc atcagatgat cttgactgtg gaccgtcaaa agcgtgactt caacgtgatt
     8701 tccttgtgct tcttgtgtca gcatctctgt ttgagagtga tgtgcttgga ctaaagattc
     8761 ctctgcatct ttgatttttt catctgctaa agtgaaatca ccttttttcg cagcagcaat
     8821 cgcttccatt gcatcgcttt ttgcatttcc gccaaacatg atcagaccca tgatggcttc
     8881 tagatttttt tgatcttcca tttattgtat cctccgataa tttttattat ttgtttatta
     8941 gtttttctgc ttggtcgagt actttttcac cattcatcat gccatagtca gccatattga
     9001 ttacatctaa aggaatgcct ttaggtgcta gcttttcagc aaattgattt tgcataaagc
     9061 gtacttgcgg tcctaaaagt aaaacgtcta catttttaga ttctagttga ttatccgcat
     9121 ctgaagcaga tacagcaaag atgtctgctt ccattccttt tgcttctgct gccttttgca
     9181 tttttgtgac taggagactt gtactcattc cagctgaaca tacaagcatg attgttttct
     9241 tttccatttt aaacacgttc ctttctatac ttacacttgt atataagcaa gtagcgtgcc
     9301 aactttaaaa gcgctttaaa aagttgatat catcacattt aaggtctata ccaagttgca
     9361 tacacactaa aaaagaaagc gcacactaaa cacacaaatt aatttttatt accttttttc
     9421 ttttaacgtg cacaaaaaaa gttgtgacac aaaacttgtc acaacttttt tcaaataaac
     9481 ggtattcaaa agcttgcatc tgcggtaata agtccaacca agttaaaaac ataagatgtt
     9541 gtttttactt tgctattctt attacttgat gcaggacagc tttttcacaa cctctcaata
     9601 aacggtgttc aatcagctga ccctgggtat tagctcaaaa cgctggattc acaacctctt
     9661 ttatttcaaa taaccagtaa atacaatgaa catctacaaa aaacttctat ttatttatag
     9721 atttcgtcag gcaaatccaa ttctctctta tcaatcaaag tctgatacca cgaagcagat
     9781 tttttcaatg ttctgcttcg ttcatcgttc aattcgattc gaactaatcc atatcgattc
     9841 ttaaatgcat tcattggtga cacacagtca gtgaatgccc ataacatata gccttcgcaa
     9901 tttgcgcctt cttctgttgc tttgatcaag taatgcaaat gctgtccgat aaattcgata
     9961 cggtaatcat cttgcacgat tcctttctca tccatatact gttcttcata ttctcttccc
    10021 ataccgtttt ctgttatcag ccaagggata ttatgataat tttcttttag atacatggct
    10081 atatcataca tgattttcgg atagatttcc catcccctgg aattattcat tttcttccca
    10141 ggtaaatcaa agacttcata atatcgttca ggatggaaag gtgtctcttc attccattga
    10201 taacgagggg cgcgaacgcg tttaggataa tattgattga ttccaaggaa atctactgta
    10261 tgatgctgaa tcgtttctaa atcctctgtt gcaggatcga aataaatcga ttgtttttga
    10321 cacaactcca ggaactcttc tgtgtagtat ccttttacca tcggatcaaa aaatactcga
    10381 ttataaaaca aatcatacat ataagcagct ttttggtctt ctggagaatt ggatcgagca
    10441 tagaccattt caggatttaa aatacagcca atccttccac cataatttcc gctgcggtaa
    10501 atctctactg ctttagccgt tgctaaaact ttatgataat tccacatcat ccactttttt
    10561 gtgttttgtt catgcggcca gcgaacagca tccaaatagc aacgcgtttg gggaacgatt
    10621 ggttcgttga atgtaaacca gttttttacc ctatcaccgt aacgatcaaa ggcaatttga
    10681 gcatacttag cataaaaatc aactactttt cttgaagacc agccatcata tttttccaaa
    10741 agaaaagctg ggacttcata atgttccaaa caaatcatcg gttctacgtc tgcttcgatc
    10801 aaagagtcga tcatttcact tatatgctga gcatattctt catctactat acagttctca
    10861 taatcagtaa aaaaacgagc ccaattgata gaagtccgat aatgtgtcag atggatggat
    10921 ttcatcagat ccacatcctg ctggtattgt tccataaaat tagttgctac ggctgggcca
    10981 tacccattat gccaaacaaa atgttcattt ttataccagc tatctataaa cgaatcctgt
    11041 ccttcttttt ttcctttcca accttccgtt tgccaagcag aagatgcagc accaagaata
    11101 aaattttttg gaatagtaat tttcatgatt tcctcctgtt ttttagatcc catcattttc
    11161 cacacgattt gctactttta caaatggcag atagatcaaa atagaaacaa taatacaaac
    11221 aagttgcgta ataactgcac cgatatttcc tgctgttgcc agataagcat tgacgatcgg
    11281 cggcattggc caagggacca ttacaactgc tttcgccgca aatcctaaag cggttgcaaa
    11341 ataaccaatc gttccagtga taagaggcgt taagataaac ggaatagcta aaattggatt
    11401 cagcatgatc ggcataccaa aaataactgg ttcattgatg ttgaagatcc caggagcaag
    11461 ggataacttg gtgatttgtt tcatatcttc ccgttttcca actaacaata ctgcaatcag
    11521 taaaccgatc gtgactccag acccgcccat actcatatac atatcccaaa atggcatagt
    11581 aataatgttc ggtacttctt ttccagcttc aaaagcttcc gtatttacag caattgccgc
    11641 taaaagcaac ggttcccgta tcggcttcac catttgattt ccatggatac caatcaccca
    11701 aaagacttgc gcaataaaca tcaatagcaa aatacccggt aacccttgta cgacaccttc
    11761 taatgggcgt tgtacgacat tgtaaatgat atcataaagg tacattcctg taagttcttt
    11821 gatgataaat cccgcagccg cgatacccga tacagtcaga atcgtcggga ataaagcaga
    11881 aaaactagca gaaacattcg ttggcacggt atcaggcatt ctcagctgta gtttcttctg
    11941 tttcctcaac cagcaataaa gttcaataga aaggatggag accaacattc ccaaaaataa
    12001 ccctttcgta tctgtatatt gtttagccaa tacgttttct actataacag tctgattttc
    12061 attcactaat tcataaacag ttggattaac agataaataa gacatcactg cgatgatacc
    12121 agggaataca ccatccactt tattcagctt ggctaattcg atccctatca gaaaaacagc
    12181 atagatggtc aagaaactta gtgtgacata gctgatggca gatgcgatag gctttaattc
    12241 ttctaaaaaa cgcaagaaac ttatctgtgc taagccattt tccgagtcaa agaccattgc
    12301 tgaaaacaat gtgccaaaag cacccgtaat aattaccgga atcaatgcag caaatgcgtt
    12361 ttttatcacc atgatatacc ttaaattatt gattctcgtt gccacgttcc ctaaaacatt
    12421 ggctaattta tccataaaac tcattccaga tcccctcatt cctgcttctt ctccttgacg
    12481 tcatctcttt ttgtctatta gtcagcgctt acatttcgat gaacagaaac tatttactcc
    12541 tcttttttac caaatataaa acaaaaattc atctgatgta tttatcataa aatacatcag
    12601 atgaactgtc aattagtttc tttttctcct attattttct gtaattctct ccgatttatt
    12661 gccatatgga cagtcattgc atcaaacgca gcaagtgcat tccgcctttc gatggcctga
    12721 acgatctctg tatgaatatc gatcgtctcg attttcagat gctggatatc ataattttta
    12781 ttaaacaaag caattgattc attaatgatt gggataatat gcagcaaggc tacgtttccg
    12841 ctagcttctg caatcatcgt atgaaaatcg atgtctaatt gaacatgttc ttgtgtgcct
    12901 tctcgaaaag aagtttcaat cttttgcgtt aattttttca tttggacgat ttgattcttc
    12961 gttgcatatt ccgctgccaa acctgccatc ttaggctcca ataaatatct cagttcaaat
    13021 aagtctcgta tcatccgatc ctgatcttca atcaaagaaa aaccgagtgg gtcattggca
    13081 atccctcgct tatcactgac aaatgtcccc gctccttgcc gaacttcaag gatattcctg
    13141 gaagctagag cccgcacagc ttctcgcacc gtgcttcgcc ccacctctaa ataatcggac
    13201 aattcatatt cattcggcag tttatctcct acttgcaact gcatttcctt taaatatcta
    13261 aggatcttgt cagtagttac ttctactagg gttttatttt cttccaaacc acatacctcc
    13321 ttttcatctg atgtattttt tcgtaattat tataacacgt ttgaatcatc tgatgatttt
    13381 ttgtatacaa aattgattaa tccgttcata gacatacaaa cgaagaagat atttaccata
    13441 aaaaagacta ggataaaatt cactcctagc cttcgatcat ttttattttg aacgacaaaa
    13501 aatatgatgt tcaataacag acgatcttca ttcgattctt gatgtcatct tttgtttttt
    13561 ttaatatatg gataaaaagt ttgattacat cgtttctatt ctagaatttg gtcgattctc
    13621 aattcttctt ctggtgagaa atctaaatta tccatcattc gaacactttc ttgtaactga
    13681 cttaaccgac ttgctccgac cagtacactt gtaacagatt tttgtcgcaa attccatgca
    13741 agggccattt gcgctaatga ttgtcctcgt gatacggcga ttgtttgcag tgctttgatt
    13801 ttttccagag ttgatccaac ttgttcttct gaaaggaatg gtatttcctt acgatgtgct
    13861 ctagaatctt caggaatacc atgtagataa cgatttgtca gcaacccttg agcaagcgga
    13921 ctgaacgtga ttgcccctag cccttcttct tctaatactg gagatagatc gtcttctagt
    13981 agtgcacgag aaaacatatt gtagcgcatc tgatgaataa tgaacggtgc ttcctttctt
    14041 tttaaaattt cagtcatttt ttttgtatct tctccattat agttggatat accaatatat
    14101 aatgcttttc cttgtcttac aagttgcatc aaagcttctg ctgtttcttc caaaggtgta
    14161 tcagggtctg ggcgatgatg atagaagatg tcaacataat ctagccccat tctttggaga
    14221 ctttggtcac aacttgaaat gatggattta cgagagcccc attccccata tggcccttcc
    14281 cacatatgat aacctgcttt gctagaaata atcagttcat cacggtaggc ttgaaaatct
    14341 tctctaaaaa tacgcccaaa gtttttctct gcagctccag cagggcctcc ataattattc
    14401 gcaaggtcga aatgggtaat tcccatgtcg aatgcgcctc ttaaaatctc tctttgatta
    14461 tggataggat cataatcacc aaaattttgc cataatccga gagataaaat cggtaatttc
    14521 aaacctgaat gaccggattt acgatattcc attttttcat aacgtgcact atctgcaata
    14581 tacaattttt ctcctcctca tcaaatcttt ttaaaacgct ttcttttttc tatttttagt
    14641 ttactacatt ttaaagaaaa ggagttaatc aaaatgattt tttcgtatat ttttttgcca
    14701 tttgtttttt actcgttgat agatcagaaa ttgattacgt gcttgctgga tttttttata
    14761 acgtttcggt tgttttttgt aaaattgctg atgatagttt tctgctggcc aaaacgtacc
    14821 ggctggttcg atttttgtga cgattggttg gtggaaagtt cctgaactgt ctagtttctg
    14881 ttttgattgt tccgctaatt ctttttgtcg ttcgacttga taaaaaatca ctggacgata
    14941 ttgcttccct ctatcttgaa attgtccaaa tgtatctgtg ggatcagtaa tttgccaata
    15001 gattgtcagc aactcgctat aacttatttc tcttgtgtca taaatgatct ccacagcttc
    15061 cacatgacct gtgtaaccac cacttacttg atcataattt ggtttttcaa catgtccgcc
    15121 cgtgtatcct gacaaaacag ataggatccc ttttttcgat tcaaacggct cgaccataca
    15181 ccagaaacat ccccctgcaa atactgcacg ttcttgatag tcagcatctt gcatggcatg
    15241 cttggaaaag tcatattttt gctccttatc aaatccttcc gtaatcaata aataaaagtc
    15301 cattacatca ggcgtcagat tcattcggat agctaatgga cgtagtgttg cttctagttt
    15361 tgataactgt tctttaacgg atatattttc tcttatgtca ttctttgtct gaatcaatac
    15421 ttttcgttcc caatctcgtg tattcggatt caaaatcaga ttataaagtg tttgaatgaa
    15481 tatttgttcc tcatccatag attctcctcc tttccgaaga agacaagcta gtgactttag
    15541 ctgtaattta tcataaagtg aggaaataag cgattacttc atccccctac agatttttcg
    15601 tataaaaaag agagaatcct tatcgacaga ttctcctttt taatcaattt attttataaa
    15661 tgaaaagtta atttaccttt tatggaaaac tattttaaaa aataaacata aatatgcagt
    15721 taatgtaaca agataagaaa taatagtcgt tactgtataa cttgtgctta ctttaatatc
    15781 agcaactcct ttgactccgc ttatttgtac caatccatta gcattcacct gctcctttac
    15841 aggttgatga tttatttcta cattcgttcc taaataagca tatacaggaa gtgtgattac
    15901 ttggctcttc tcattagtta ctctatatat gatagaacta ccagttgttt tataaggaag
    15961 gtttacccat tgactgccaa tctttgcttc gtgttttatt atatgctcct tattctccaa
    16021 tgaagcgata ggagaataat catataaaaa ataatccatt cctttagtgg tatacgcct
//
LOCUS       DAYYCY010000054        16033 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000054 DAYYCY010000000
VERSION     DAYYCY010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16033
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.054"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            111..779
                     /locus_tag="ACHAZB_001358"
     CDS             111..779
                     /locus_tag="ACHAZB_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFM8614043.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFSLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(979..1365)
                     /locus_tag="ACHAZB_001359"
     CDS             complement(979..1365)
                     /locus_tag="ACHAZB_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8614044.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(1337..2719)
                     /locus_tag="ACHAZB_001360"
     CDS             complement(1337..2719)
                     /locus_tag="ACHAZB_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFM8614045.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(2875..4062)
                     /locus_tag="ACHAZB_001361"
     CDS             complement(2875..4062)
                     /locus_tag="ACHAZB_001361"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFM8614046.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4069..5100)
                     /locus_tag="ACHAZB_001362"
     CDS             complement(4069..5100)
                     /locus_tag="ACHAZB_001362"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFM8614047.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVGIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5116..6570)
                     /locus_tag="ACHAZB_001363"
     CDS             complement(5116..6570)
                     /locus_tag="ACHAZB_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HFM8614048.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEELENKLAPADTMEDELHDAVVPSH"
     gene            6745..7650
                     /locus_tag="ACHAZB_001364"
     CDS             6745..7650
                     /locus_tag="ACHAZB_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HFM8614049.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            7741..8418
                     /locus_tag="ACHAZB_001365"
     CDS             7741..8418
                     /locus_tag="ACHAZB_001365"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFM8614050.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8415..8585
                     /locus_tag="ACHAZB_001366"
     CDS             8415..8585
                     /locus_tag="ACHAZB_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8614051.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            8699..9595
                     /locus_tag="ACHAZB_001367"
     CDS             8699..9595
                     /locus_tag="ACHAZB_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFM8614052.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(9650..11020)
                     /locus_tag="ACHAZB_001368"
     CDS             complement(9650..11020)
                     /locus_tag="ACHAZB_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8614053.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11258..12004
                     /locus_tag="ACHAZB_001369"
     CDS             11258..12004
                     /locus_tag="ACHAZB_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFM8614054.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQPVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12001..12840
                     /locus_tag="ACHAZB_001370"
     CDS             12001..12840
                     /locus_tag="ACHAZB_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFM8614055.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            12841..13791
                     /locus_tag="ACHAZB_001371"
     CDS             12841..13791
                     /locus_tag="ACHAZB_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFM8614056.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            13932..14684
                     /locus_tag="ACHAZB_001372"
     CDS             13932..14684
                     /locus_tag="ACHAZB_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8614057.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            14703..15854
                     /gene="queG"
                     /locus_tag="ACHAZB_001373"
     CDS             14703..15854
                     /gene="queG"
                     /locus_tag="ACHAZB_001373"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFM8614058.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEVYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         5197 a         2994 c         3220 g         4622 t
ORIGIN      
        1 tgtcacagtc ctttttttag taaaaggata catttcttcc ttgtatattc catgcgtgtt
       61 cgtgagaaag catatataat ttaattaaca gatagttagg gggagaaaaa atgatttttt
      121 caattacaat gaaactggtg ttcgggttga tcggtttatt attagtggta cgtttgcttg
      181 ggaagaaatc catgtcagaa atcacaccgt ttgatttagt ctatacgctt gttttaggtg
      241 ggatcttaga agaatcaacc tatgatgaca atgtccatgt agggcatgtc ctatttgcca
      301 ttgcgctttg ggcagtaatg atttatggta tcgaacgtat cgtccaaaaa aatgaaaagg
      361 tcaatcgttg ggtcaaaggc gagccatctg ttctaatcaa agacggcgtc atcaacatga
      421 cagagttgac aaataaccat atcgagatgg agcagttgcg agcaatctta agacagcaag
      481 aatgcttttc tcttgaaaat gccaaacatg ttatcctaga gaatgctggt caaatgagtg
      541 tattgaaaaa atcagatgaa gacaaagctt tatcgatcct gcttgtagat gaagggcaaa
      601 ttcagcataa agtgcttcag tccaatcaat tgactgaagc atggcttatg gagaacttga
      661 agaaagaagg ctatgcagat gtaaaacagc tgatttatgt tgaatggtca gaagaaaaag
      721 ggctctacat actaaataaa gaagacgtca tgcatgaatc ttaccgaata gatggataga
      781 tagacagagg ttgtgaaatc agcgccttgg cttgttactc aaaactaagc gactttttca
      841 caacctctga tatctaatat acgttgttcc agaagttgct tcttcgaaaa ataagccaaa
      901 attccacaaa aatttaagaa gcaattttcg aaaaatcctt tcttattttt tgaagttgat
      961 cacttctgtc acaacctctc atgtcacaaa ccattttttt gtttccttta actcaatata
     1021 ccgtacgtct tcccacaata tcggctgatg gtcaatcata aagtaatctt cataggcttc
     1081 accaatgaag tgcccttcga cctcatccaa gagtcttcca aattcatctt ttaaattgag
     1141 ctgaatgatt actgctttac gagataaaaa ggcttctgtc agtatactat cgatttcttg
     1201 caatgtcatt tgttgtttag gaagaggctg tttgcgtgct tcttcttcat tttttttgat
     1261 accgttgact agttcttcca tcgcaaaagc agtcccccat tttaatccaa atggccgatc
     1321 tcgatactcg ttatagtcaa tgaatttttt tgctgtccgt ctcatctttt ccaccttcta
     1381 atccactcat accacctgca tgcccgccaa cgagagaact acgtgcgatg gcgcgtcctc
     1441 cttgcaagag actatgcgca tggatgattg attgaaagcc gtattttttt ctaatcgtat
     1501 cgacaacaaa atcaactttc gcttcattta tctgcttttc aggatcttca aaaaggttaa
     1561 gctgtaaacc tgtcgcgtat acgaggtcgg aacagttcac gcctacacgc cgaatatcct
     1621 ggtagtggta atgctggtgg aaaatggtca ataaagcatc tgctagatct ctgctagcat
     1681 ttgtcgcttc gattttcatt tgctggtgaa acccggtctt gccatttgga tcaagatagc
     1741 ctaaagagta tcctacccat aagctgacta cttgtgtttt tgctgcagat cgtctcaagc
     1801 gagtgccgac ttggtcggcc atttctttga taacgatctc gatttcttct cgtcttgtat
     1861 aatctctatc taggatttga ctatttccga tactttttga ttttggtgta tattcttgtc
     1921 ccaaaaaact tcgatcgatg ccccaagcat gggcataaag ctgagtaccg atgaccccta
     1981 gcttgctttt caagtgataa taatccgcgt gagctaaatc ataaacagaa tgtataccca
     2041 tttgtttcag acgttttgct gttcgtttgc cgattcccca aaagtcagtg atatctgaga
     2101 ttttccataa cttttctggt acatcttcat aacgccattc agcaaacatt tgctttccgt
     2161 cttttgagcc attgtctaag gctagtttag caagtagcgg gttatcgcca attcctatag
     2221 tcgtgtagat tccggtctgc tcaaacacat ctttatggat cagtttagcc atgtcgtacg
     2281 catttgtttt accaaagagt gccatgctat ttgttacatc aaggaagctt tcatcgacgg
     2341 aatagacatg atggttcgct tcgtctgcat attttttata gatagaattg atttctctgt
     2401 tcttttccat ataataggcc atccttgggg caacaatata taaatcactt ggatagggga
     2461 agggaagatc tctggcacgg ctgatattgg tgatgccgta agcttttttt gctgcaggac
     2521 tggaagccaa gattaatccg ctaccccgtg tttctgtcga atcggacgga taggacatga
     2581 ctactaattt agttgtaagg ggatcaagat ccttttcaac gcactctacg gaggcgtaaa
     2641 aagatttgca gtcaatacat aaaatatctc ttgaaggttc tttttcataa acaaataccg
     2701 gattttgatt cgcccacata acaatcacct cttcaacaca attagaacac acgttctctt
     2761 tccgtgtcaa taaaaaacag caattttcaa atacgagcaa aaggctcaca tttggaaatt
     2821 gctgagaggt tatttttatc tatttgtttt tagctgagat cgttgatttt tttatcaaac
     2881 atgaacagct tctggatcga tctttgttgt cccatttcca atgtgtgcaa cataaaattc
     2941 cggagcatag ccgacaactt tcatgtattc atcatagaca ttgtttttga aggcaggtat
     3001 attttcttct ttgacaagag caatcgcaca gccaccaaat ccagctcctg tcattcgtgc
     3061 acctaagaca ccttcttgtt tttgagcagc atctactagt gtatctaatt cgatcccagt
     3121 tacttcataa tcatagcgaa gagagtggtg agaatcattt aacagttgac caaatgcttc
     3181 taagttccca gcttctaatt cagcttttgc cttcaaagtc cgctgatttt cagaaacggc
     3241 atggcgagct ctgcgtatca atgtatcatc gccgatttgg tcaatagttg attcaaattc
     3301 ttcttcatct aagtctccta aagaagagat aggcaaagtt ttttgcaaac gtgccaaggc
     3361 ttcttcgcat tcgctacggc gttcattgta ttttgaatct gccaattccc gccgcttatt
     3421 cgtattcatg atgacgattg cataaccatc tagtttcact ggaaccattt cgtatttcag
     3481 cgtatttgta tctaataaga ttgcatgatc tagctcaccg aagccgatgg caaactgatc
     3541 catgatacct gaattgacac cgatgaattc attttctact tttttacctg tttggactag
     3601 ttccaagcga tctgttttta aatcaaacag atcttctaat acaacaccta ctaataattc
     3661 taatgaagcg ctactggaaa gtcctgcacc attagggatt gttccttcca ctaacaactc
     3721 aaacccatga tcgattgagt gtccagcttc ttttaattta aggaccattc cttttacgta
     3781 atttgcccag tttgcttttt tatcatattc tagctcatct aaagtaaaac taatgacacc
     3841 gatatcgtca aagtttgtgg aataaactaa tattttatta tcgtctcgag gtgctgctac
     3901 accttgcgtt ccataagtga tggaagcggg gaagacatgg cctccattgt aatctgtgtg
     3961 ttcaccaatc aaattgatgc gtcctggtga gaagtattgt gtagtttttt taggtccgaa
     4021 tgtttcggca aattttgtgt ttaagcttct tttgatttcc atgatgagtt attcctttct
     4081 tgttttgatt ttgaattctg tcactgtttc aaatactgat ccagctttta aatgaatcga
     4141 accaaatgct gagaagcgct ctgcacctgg agctgtttga gtctcaaatg tgatgccacc
     4201 gtgatgtgcc agttttcggt tgcgcatttc tggtgtttct gtgccgaaat ttgcagtgaa
     4261 aataaccaca ctagaagcat ctgtagcaat acctacttgg attcttttat cggggctagt
     4321 caagcgagcg gcttctttac ccaagccggt ttcttttaag aaaaatggat gatcgattcc
     4381 gtcaacaaga tttttttgat ccaaatcact agcaaaaacg gacgctaatt ttttaggtat
     4441 ttgaaaatcg aaagcacttc ctgcagcatt ttcttttacc cctataggaa tactatctgt
     4501 tcgcaaagga gcataagctt cgctattgag ccaaagctca tgttgatcga ttgactgaga
     4561 agcatctccc gtcaagttga agtaaacatg atttgtcgga ttgaataaag tatcttggtc
     4621 actggtgccg cgagaggtca ctcgccagat attatctttt gtcaaagtat agcggatttc
     4681 cacatccatt gtacctggaa aaccatgttc tccgtcggga cttgtagtag taaaaatgac
     4741 agaagcttca ttttcgccat tcaaaatgac ataagaccat ttttttaatt caaatccagg
     4801 agcaccacca tgcagattat gtccagtttt tggatctgtg gctaattggt aagtttttcc
     4861 attgagagaa aagcggccat tttcgattct ccctgcggtg cgaccgatag aagcaccgat
     4921 atacggatct ttttcgatat attcttcagc ggtgtcaaat cccaaaacta attctcgatc
     4981 atttgatttt aaactgacga ttctagcacc taaatcagag atagaaagtg ctagcttgtt
     5041 tttgttctcc aaggttatca agtgataacc ttggccaaaa tctttttctg taatcttcat
     5101 tttcaagatc ctttcttaat gggaaggaac tactgcatcg tgaagttcat cttccattgt
     5161 atcagctgga gccaatttgt tttccaattc ctcaacgatt tgtgcatgtt ttttctctgt
     5221 caatgtaatc ttccaagcaa aaataatagc ggagaaaatc atcaaagcag caggagcata
     5281 gaatgcataa gctttgaaag tcgttacgcc atgagcagtg atatcagcag cagtagcatt
     5341 accagtcatc ccagaagcga tagcgacaaa ccctacgatc ccgttagaca aagcaccagc
     5401 gattttatct agtaaagggc gaattgatag agtaacagct tcgttacgga taccattttt
     5461 ccattgacca tattcaacag agtctgtgat cgtcatcaaa gcggataaga aaatcaattg
     5521 ttgagggaag tagaacaata ctagcccgat gataacgatt ggcaaacttg aacctgcgat
     5581 cgtaaagaaa aagtaagaag aaatcatcat tgcgatccct ccaaggtaaa cgtagcgacg
     5641 agagatgacg cgtgtaagca ttgggaatag tggaacagct actaaaccaa gtaaggcagc
     5701 aactagacca acgatccaaa attcagttgg ttggccaagt acatatttga agaagtagaa
     5761 taatacggca gttgttgcta cattggcaat cgcataagcg atataagcaa gggcaagcca
     5821 catcaactga tcattttgag caactgcttt gaacacatct ttgatcccag ctttttcatt
     5881 ttgctgacgg atcgcagtat cgacttcttt tgtgcccaag gcagtgataa tcgcagtacc
     5941 gcctgaaata acagcagcaa tcacaccgaa ccagaaccag cctgaagcgc cttcaatatg
     6001 tttatgagta acaagatagg taaagaaagt aacgataggg acaacgacta gagtagtacc
     6061 atttgcacct agagaagagc cgaaacgagc aaacgtagca aatttttcac gttcctttgt
     6121 ttccgtactc aaagctggaa tcattgacca aaaagcgata tccttaaatg aataaaaact
     6181 atccaataag atgaaaacga ttgcaaataa tatgataaat aaagtcgtat tatgagtagc
     6241 taagccaaag aaattggtaa agattgctac caagagtacg gaactaacaa cgccgccgat
     6301 aactaaccat ggtttgaatt taccgtattt tgtacgagta ttatcgatga tgccaccaat
     6361 caaaggatca aaaataattt caactaagcg gatacctaca actaagcttg taactgtagc
     6421 aatcattttt gcattcgttg ccttatctgt tcctgcgaac atctggcttg tcacgaatac
     6481 cataaaataa gtgcttaatg taacatagaa tgtgtcgtgt ccaaaagcgc ctaaagcgta
     6541 ggacacacgt tggatcattt tgcttttcat aatctgtatt cctctcctgt tcaatatata
     6601 atactaagta aaccggttac aacgaataac aaagatcttt taatgactta aacttaacaa
     6661 gaaatcgctt cctttttaat atatttctgt gacttttatt taactttttg ttgcattata
     6721 ctaaaggagt aaggagggat agacgtggag caatcgattt ttttaaaaaa ggaacatcaa
     6781 caagtagaaa aagaattgta ttttgatttt tgtggtttct ctaaaacgat gccgtttcat
     6841 tcttttggtc ctgctattcg caatgattac atcctgcatg tcgtgatgga aggcaaagga
     6901 tcttatcatg tgaaaaatca acaattccaa ttgaaaaaag gtgatctgtt cttgattcgt
     6961 ccaggagact cgacgtttta cttatcagat ggagaagatc cgtggatgta ttgctggctt
     7021 tcatttggtg ggagtgctgc agaagaaatc attcggcagt cgcttttcag agaagaccaa
     7081 tacacgatgg tctcttctga catacaaaaa tatatcgaca ttattttaga atgcatgaaa
     7141 gatacggaag aaaccttgga aaatgaatta cagttaacag cattaaccta ccgtttttta
     7201 tcagtccttt taaaagatgg cggaaaggtc agcggtggtg tacaaaaggt tcattcctct
     7261 ttagctgttg aaacagtcaa atacatcgag gcacattatg ctgaaaaatt gtctgttgag
     7321 gaaatcgccc agcatttagc ggtcaatcgg agccatttgt cgcgagtatt caaaaatcat
     7381 atgggtacca gtatcaagga atatttgatc ggggtacgga tcaatcgagg ggcgtttctt
     7441 ctttctctga cggatgaatc cgtggaaagt attgcctatc aagtagggtt caatagtcta
     7501 gtcgtattta gccgaatgtt caaaaagagt accggagaaa cggctacgaa ctatcgtaaa
     7561 cgtatgaaaa acgaaaattt taagaatatt gctttggata ccttgaaaaa acagttggaa
     7621 aaacaaaccg cagtttcttg gacaacctga ttagtctggt taaaatgaca agcatcggca
     7681 aaagaaactt gtggcggtta tgctacactg taaagcgaag actaaaaagg aggaaaaggg
     7741 atgcgtacac ttatcatcat tagccatcca acgatcgaag aatctttaaa tcagcaattt
     7801 ttcaaagaag cttctgctgg tttaccagct acttggcatc atttggaatc aagttatcca
     7861 gacggaaaaa tagatataga gactgaacta cagctcttgg cccagcatga tcggatcatt
     7921 ttccagtttc cgttttactg gtatagttct cctgctcatt taaaaatttg gcaagataat
     7981 gtcttggaac gagcagcaca tgtattaaaa gggaaagaac taggaatcgt attgacaaca
     8041 ggtgtggcgg aaaaagaata ccaagctgga ggaaaagaag aatatacgat ttccgaattt
     8101 ttgaggccgt atcagcgaat cgcaaataaa ttccatatga cctatcttcc gccattcgtt
     8161 ttagcacaat ttatgtattt atcccaggaa aaacgttggg agaagctaat tgcttatcag
     8221 cagtatctct cattggaagg caaaccatcg ctgactcagc ggattgactg gtttattcaa
     8281 agagtacagg aaaatcagaa aatgcaggaa gaagaatctg aaaaacaagc atatatcgta
     8341 gaagcactga ctgaggcaaa agagcagatc gaagacttga cctttacact gcaagaaatg
     8401 aaaggaactt cattatgaca aatgattggc tgatccaaca attagatgaa ttaagcgaaa
     8461 aatcagaata tcaagaccgt gcgctcttat atgaaataaa gaaactgatg gctgagatag
     8521 acgaacgaac aatgcagctg caaggagaaa tcgacggaca atcttggaat catctcaact
     8581 ggtgaaattt tcttataaat aacaaagata aaaatgtgca cagcgacaat tatttgctac
     8641 aataagaata tgaagcgttt gataacgcta ttatacataa aaattggtgg tgaaaaaaat
     8701 gtatgatgaa aatgagtggg gagaaaatgt tcggatgacc cgaagaagac agcaaaaaca
     8761 ggcagctcgt aagcggatga tcatgattgg ggcagcagtt gccgtgtttt tgatcatagc
     8821 aggaggagtt gcgggtctat attttcatgg aaaaaattcg tcagaagcga aaagtacgac
     8881 cataacggaa acaacgacaa agaaaacaga gacgaaacaa acaagtgagt catcaactaa
     8941 aacaaaagag acacaaactt ctgaagaaaa aacagcaaat ggtttgccag agcaacttca
     9001 agcaatcgta aatgattatt cgaaaaagat cgaagaaaag acgcctgcat tgattgaaga
     9061 ataccagaca gaaattcagg gaaatcaaga gggaatagct ggtttatcag cagttgctaa
     9121 ccaaaaagca cgtgagcttc aagcaatctc cgacgaaggg atcagaaaat tacgtgcagc
     9181 gtatcaggca gcagaaaata aagacggtgt agacttagat actttgatca atcaattatc
     9241 ggctaattat acgaatcatg tcgctaagat ttccgatatt tatttgcaga ctagcgcctc
     9301 tcttcaagca gagtcaacaa gcacacaaga cacaacatct tctgattctg agacagcaga
     9361 gtcggtagaa gcttctgaaa tgcaagctcg aacaacacaa gacacaacag attcctctga
     9421 aactaatacc aacagcgcga cttcacaggc tgcgacaacc gtagttttgg aaggagaagg
     9481 tccaaatcaa atagccgaac gaactggcgt accagtagaa acgatcttgt cgctgaatgg
     9541 gatgagcatg gagaattatt tcctgactcc cggtgagacg ctaaaattga attgataaaa
     9601 gcaataaaaa agaacgaagc ttccactaag gtgaagcttc gttctttttt tattttccgg
     9661 ttcttttttc gcgtacaaag taatggatcg tcatgtaaag caagaaaacg agtccaataa
     9721 tcaatgagat tctcgtttca ggattgataa acatgaaaat caatacgata gctaaaaaag
     9781 caagtgtcac ataattcgta taaggagata gtggcatttt aaaaggatga tcatgcattt
     9841 tatctgcgtg cttctttttg aagcgaatct cgctgatcaa aatcactacc caaggaacca
     9901 taccaggcaa aatacttgag ctgtatacga aaacgaaaat attttctgac tggttgatga
     9961 acattggtag aagataattt aagatcagac caattaagat tccacctgag attgctacga
    10021 caggataaaa aggcgcaccg tgacgagata gtttcagaaa ttttttagac atctttccat
    10081 ttatggcaag tgtatagacc atacgacttg cgctgaaaat ccctgaattg caacctgata
    10141 atgccgctgt aatcacgaca aaattaatca gtcctgcagc aaatgtaatg ccgactttag
    10201 aaaaagtttg tacaaaaggt gaaccaatct cactcaattg attccacgga tagatactca
    10261 caatgataaa aatagcacca atataaaaaa tcaagattcg accgatagtg gactggacag
    10321 cttcaatgat cgtcttttta ggattctctg cttcaccagc agtcattccg atcagttcga
    10381 ttccttgata ggaagcaaca acgatcgata atgcaaagaa aaatcctttg acgcctccag
    10441 tgaagaatcc accatttttc caaagatttg aaatccctac tgcctgacca tgattaccaa
    10501 atccaaataa gattacccca agacctgcaa tgatcatcaa aatgatcgtt atgactttga
    10561 tgatcgaaaa ccaaaattct aattcgccaa acgccttgac agaaataaga tttgccagca
    10621 taagaaacaa gacgacaatg attccgggaa taattgccgg caaatcaggc caccaataat
    10681 tcatataaga tcctacagca atcacttcac ttattcctac gacaatatat tgaaaaatat
    10741 tactccaagc ggtcaagtag ccagccacag gatggatgta ctcgctcgcg taatttgcaa
    10801 aagaaccagt cgaaggatca acgtaaagca tttcgcctaa agcacgcata atcaaataca
    10861 aaaagaatcc tgcaatcgca taggctccca tcactgaggg ccctgtccaa cgtatggttg
    10921 aggaggcacc cataaacaga ccgacaccga tcgttccacc tagtgcgatc atttggatat
    10981 gacgggcact aagacttcga gttaactttt cttcttccat acaaaaactc ctttcgttgt
    11041 tgagtcaaca agaaaaaata atcaaacgtt tcaacacatg tttttcatta ttaggttagc
    11101 cttattgaac tcttgatgtg attctacaga aaattttcat aaaatgcgag gaaaattcga
    11161 aaaaaacttt ttcatcattc tacttgaaaa tgctttccat tttagttaaa ctatatttaa
    11221 ataaaaagtt aggtaaacct aaaaaggagt gtaagatttg gaacgtacgg ccattgtaat
    11281 tcaagacctt tcagccgctt atcaagggaa aactgttttg aaaactatca acttgacgat
    11341 aaatccacaa aaaattaccg gtattatcgg accaaacggt gcaggaaaat caacatttat
    11401 gaaatctttg ctagagttga taccagctac tactggtaaa gtcacctttt ctggacagcc
    11461 ggtaaactca gtccgtaaaa aaatagccta cgtggaacag cgaagtgaat tggatctttc
    11521 ctttccaatt gatgtattag gcgttgtact tttaggaaca tatccatctt tacgaattgg
    11581 acaaagacct gggaaaaaag aaaaagaacg tgcaagacaa gctttgaaaa aagtagggat
    11641 ggaagaatat gccaaaagac agatcagcga actatcgggt ggacagcttc agagagtttt
    11701 tattgcaaga gctctagccc aaggagcaga atggatcttt ttagatgaac cattcgtagg
    11761 gattgatgcg ttaagtgaac gaaagatctt tgacatcttg caggaattga agaattcagg
    11821 aaaaacgatt ttgatcgtcc atcatgatct tcataaagta gaagaatatt tcgatgaggt
    11881 tattctttta aataaacagc tgatcgcttc cggtccagta caagaagtct ttacatcgaa
    11941 gaaccttcaa ttggcctatg gtgagatcat cagacattta gtaaaaggag gagagaaaaa
    12001 atgatccaat cctttattga tggattaatg aattatcaat tcctgcaata cgctttggtc
    12061 acttccatgc ttgtaggact tgcttctgga gtcatcggtt cttttattat tttacgaggg
    12121 atgtctttga tgggagacgc tatctctcac gcagttcttc caggtgtagc catttcttat
    12181 atgtttggtt ttagttacat ctttggagcc actgcttttg ggatgctaac agcagcagtg
    12241 attggtttcg tgactcagcg gagccgattg aaaaatgata cagcgattgg aattgtcttt
    12301 agctcctttt ttgcattagg gattatcttg atttcatatg cccagagtgc gacagatttg
    12361 tatcatattt tatttgggaa tgtcttagct gttagggaaa gcgatttgtt gctgacagca
    12421 ctcgttagtg gaatcgtctt gatatttgtc tttttctttt ataaagaatt gaagatcaca
    12481 tcattcgatc cgacaatggc aaaagcttat gggctgaaca catcgctgat ccattatctt
    12541 ttgatgttct ttttgacatt agtggctgta gtcagtttac agacagtagg aacaatcttg
    12601 gtgattgcca tgttgatcac accagccgcc acggcttact tgctaacgaa ccatttactg
    12661 aaaatgatca ttacagctgc aggaatcggt atgctaagtg cagttgtcgg tgtgtttttc
    12721 agttatagtt acaattggcc atcaggagct acgatcgtgt tagcatgtac cgcatttttt
    12781 atccttgctt ttttattttc tccaacaaaa ggaattttat ttcagaaagg aagcagatag
    12841 atgaaaacaa aaaaatcact ctttttaatc ttggcagtta gtttcttggt attagccggt
    12901 tgcggaaagc aggcttctga ccaagcggat gaaggaagta aagaaaagtt gtcagtcgtg
    12961 gctaccaatt cgatcttggc ggacatggca aaagaggtcg gaacagatca aatagatatc
    13021 cacagtatcg taccagtcgg aacagatccg catgaatatg aagtactacc agaagacatc
    13081 aaaaaggcaa gcgatgcaga tgttatttta tacaacggtt tgaatcttga aacaggtaac
    13141 agctggttcg ataacttgat ggaaacggct aaaaaagaag aaggaaaaga ttattttgca
    13201 gttagcaaaa atgtagaacc tctttattta actagcggtg aagaacatac aaaagcagat
    13261 ccccatgctt ggctagacct atctaacgga ataaaatatg tggaggaaat cgcacgtata
    13321 ttctctgaaa aagatgcaga aaatgcgaca ctctataaaa aaaatgcaga agcatatgtg
    13381 gaaaaactaa aagaattaga tacacaagcg aaggaaagtt ttgcttctat cgaagagaac
    13441 aaaaaattat tagtaacaag tgaaggggca ttcaagtatt tttcacgagc atatgatttg
    13501 ccagcagctt atatatggga gatcaataca gaaagtcaag gtacgcctga tcaaatgaaa
    13561 gcgatcattg atcagataag agcaaaagaa gtaccggttt tattcgtgga aaccagtgta
    13621 gactcaagaa gcatggaacg ggtagcaaaa gaaacgggtt tgaaaatcta tgataaactg
    13681 ttcactgatt ccatagcaaa agaaggagaa caaggagatt cttactatca gatgatgaaa
    13741 tggaatattg aaacgatcca tgaaggtttg tcacaaacaa aagaaagctg aaaagtgcaa
    13801 ggtctagctt gaggagcaga ccttgcattt tttataagag atatgaaaaa aaattatgct
    13861 gtttttcgtc gtacagatac cttttttgaa gaaattccgt tatagttaga gtaaggaagg
    13921 aaggtgtggc tatgaatttt acacgtaaag ataaatatac gattaaagaa gaagtctttc
    13981 gattgatcag ccagcaggaa aataccgaca aggatcaaga ccttcaattt cttgaagcag
    14041 aattagctaa attattcata gcaaatcaaa atgagacaac agaaccagat gaagagctgt
    14101 acataggcaa atggttgttt gatgaatttc agcctttaga tgaaaatacc ggtatcggtt
    14161 attttgtgtt cctttctcca gtcggcttca cacctggcgt attagttaaa caaaacgacg
    14221 aaataaccgt caaagcatgg cctgcattat tttcagtcat caaagcagaa ggagtaatca
    14281 aaaagatcca gtcggcctat tataaaagtc aatttattgc caacagcaaa ttaatgaata
    14341 tgttttataa tcgtctagta gaaattgcta ggcctgggaa aatcccgcaa acattcacgt
    14401 acacacagca aaaaaatctc tatgaagaag tattgtattc ccgcgatctg tctgagtttt
    14461 cgccatggga agaggtaagg ctccatgaac tgttaagtac aaaaccagtt tcattgaatt
    14521 tttttaatgc attagataac aaaagaaaat taatttcaac aaattattgg gaagtatttg
    14581 ctgcaagaaa aaaatggatg gaagaaaaga agattgccga tcagcagatc cagtttgtcc
    14641 agctgaaaaa agaaaaacta tattatgggt tatacagccg ttaacagcaa gcgaggaaga
    14701 ccatgacatt aaaagaagaa atcatacaag aaagcaaacg attaggaatt gacaaaatcg
    14761 ggttcaccac ggcggaacct tttgattcat taaaagaatc gttggaagaa caaaaagcag
    14821 ctggccatac ttctggcttt gaacatccaa atatcgatga gcgtctttat ccagaaatga
    14881 catttgatca gccgaaatcg atcatttcca ttgcacttgc gtatcctact aggatccatg
    14941 aagaagtgcc tagggatgaa aaacgcgggc agtttgctcg tgcttcttgg gggatcgatt
    15001 accatgatat actcagagac cgtttgaacc gattgatcga attcatccaa acaagagcag
    15061 aaaaatggca aaaagaagag gaatggcgtc tagctccgca agtagatact ggagaactga
    15121 tcgatgtcgc tgtagcacag cgagctggtt taggatttat tggacgaaac ggattgttga
    15181 tcactgaaga gttcggttca tttgtctatc ttggtgagat cgttactaat attgtatttg
    15241 agcctgataa accaggagaa tttggttgcg gtgactgtat gcgctgcgtg actgcttgtc
    15301 cgacaaaagc attgcttggt gacggaagaa tgaatgccca gcgatgtttg tcttatcaaa
    15361 ctcagacaaa agggatgatg ccggaagaat atcgtaaaaa aatgcgaaat gtcatttatg
    15421 gatgtgatat ctgccagtta gtctgccctt acaatcaagg aaaagatttt cattttcatg
    15481 aagagatgga accaaaagta gacgaagtat atccaaaatt aaagccaatg ctttcgatgt
    15541 ctaataaaga cttcaaacag caatttgggc atttagctgg ttcatggcgt gggaagaaac
    15601 ctttgcagag aaacgcattg attgctttag ctaatctagg tgatcgaacg gcattgcctg
    15661 agatttcaga atgtttaaca gatgtccgcc ctgtcatcag aggaacagca gcttgggcta
    15721 ttggacgtct aggaacaaaa gaaccggaaa aatggcttga tgtattagat gaaatattag
    15781 ctaaagaaac agaagaagtc gttataaacg aattggaaca tgcccagaaa ttattgcgga
    15841 gaaagttaaa ataaaaaaat agttaacatt attcaaagtt tttgtccagt aattgttgta
    15901 acaattactg gtttttttgt attttttaca tttttttaag agaatttgat aaaaatcaaa
    15961 aatttttttg ctttttttta tggattattg cagtgaatgt tacagaatct gcgtttttca
    16021 ttcaaaaata aaa
//
LOCUS       DAYYCY010000055        15954 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000055 DAYYCY010000000
VERSION     DAYYCY010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15954
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.055"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(390..812)
                     /locus_tag="ACHAZB_001374"
     CDS             complement(390..812)
                     /locus_tag="ACHAZB_001374"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFM8614059.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            complement(826..2232)
                     /gene="atpD"
                     /locus_tag="ACHAZB_001375"
     CDS             complement(826..2232)
                     /gene="atpD"
                     /locus_tag="ACHAZB_001375"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFM8614060.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            complement(2255..3157)
                     /locus_tag="ACHAZB_001376"
     CDS             complement(2255..3157)
                     /locus_tag="ACHAZB_001376"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFM8614061.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            complement(3171..4727)
                     /gene="atpA"
                     /locus_tag="ACHAZB_001377"
     CDS             complement(3171..4727)
                     /gene="atpA"
                     /locus_tag="ACHAZB_001377"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFM8614062.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            complement(4750..5292)
                     /locus_tag="ACHAZB_001378"
     CDS             complement(4750..5292)
                     /locus_tag="ACHAZB_001378"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFM8614063.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNKAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYAYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMNYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLENMRNQLNR"
     gene            complement(5279..5803)
                     /gene="atpF"
                     /locus_tag="ACHAZB_001379"
     CDS             complement(5279..5803)
                     /gene="atpF"
                     /locus_tag="ACHAZB_001379"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFM8614064.1"
                     /translation="MLNQLAIAEVGNAMLGNVIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            complement(5894..6109)
                     /gene="atpE"
                     /locus_tag="ACHAZB_001380"
     CDS             complement(5894..6109)
                     /gene="atpE"
                     /locus_tag="ACHAZB_001380"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HFM8614065.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVVALILVFGV"
     gene            complement(6162..6881)
                     /gene="atpB"
                     /locus_tag="ACHAZB_001381"
     CDS             complement(6162..6881)
                     /gene="atpB"
                     /locus_tag="ACHAZB_001381"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFM8614066.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMVISLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            complement(7183..7647)
                     /gene="smpB"
                     /locus_tag="ACHAZB_001382"
     CDS             complement(7183..7647)
                     /gene="smpB"
                     /locus_tag="ACHAZB_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFM8614067.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIIRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            complement(7669..10026)
                     /gene="rnr"
                     /locus_tag="ACHAZB_001383"
     CDS             complement(7669..10026)
                     /gene="rnr"
                     /locus_tag="ACHAZB_001383"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFM8614068.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWATELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKTRNGKSGKRE
                     GKQAGKRPERNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            complement(10029..10784)
                     /locus_tag="ACHAZB_001384"
     CDS             complement(10029..10784)
                     /locus_tag="ACHAZB_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM8614069.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLRW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            complement(10887..11123)
                     /gene="secG"
                     /locus_tag="ACHAZB_001385"
     CDS             complement(10887..11123)
                     /gene="secG"
                     /locus_tag="ACHAZB_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFM8614070.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            complement(11241..11519)
                     /locus_tag="ACHAZB_001386"
     CDS             complement(11241..11519)
                     /locus_tag="ACHAZB_001386"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8614071.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            complement(11632..12600)
                     /locus_tag="ACHAZB_001387"
     CDS             complement(11632..12600)
                     /locus_tag="ACHAZB_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8614072.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     regulatory      12815..12859
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            12883..13857
                     /locus_tag="ACHAZB_001388"
     CDS             12883..13857
                     /locus_tag="ACHAZB_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HFM8614073.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     gene            13862..14437
                     /locus_tag="ACHAZB_001389"
     CDS             13862..14437
                     /locus_tag="ACHAZB_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytS/YhcK type 5TM receptor domain-containing
                     protein"
                     /protein_id="HFM8614074.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(14504..15643)
                     /locus_tag="ACHAZB_001390"
     CDS             complement(14504..15643)
                     /locus_tag="ACHAZB_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HFM8614075.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT         4359 a         3443 c         2856 g         5296 t
ORIGIN      
        1 aatctgagcc agaatgtggt tcattctgac tcagatttga ttttgcacaa ctcatcagtc
       61 cttattgaca aagagcccaa aagtcttgtc gcagtccctt attccaaata aacggtgttc
      121 aatcatctga ccctcggtat tagctcaaaa aacggcaaaa tatgaggaac tattttccat
      181 ttgtttctct aatactcagg acaaaacgct gatttcacaa cctccgatat acggtgttcc
      241 agaagtagct tcttcgaaaa ataagctgag atttcacaaa aatttgagaa gcaattttcg
      301 taaattcctt cttatgtctt gaagctgacc acttctgtca taacctcttt ttactgttct
      361 taaacacgga taaacatccg catctcattt tatgagtgct tagatacttt gatacgattg
      421 attgctcggt gcaacgcaac agttgcccgt ttcaattcgt tcgtatcttg ttttgctttt
      481 gcttcttcga tttggcgttc tgcgcgttgt tttgcacgtt ccgcacgaga tacatcgata
      541 tcacgttcac gttccgcact atcagcaacg attgaaacaa cattatcacg aacttccatg
      601 attccgccat taacagcgat ccaatcgacg tgggtatctg aatctgttcg ttttacacga
      661 acttcatcaa tcgtcaatgg aacgatgatt ggtgcatgtt taggcaaaat cccaagctca
      721 ccagctgtcg tacgtgcaac gacgatagct gcatggtgat catagaccaa accggcagga
      781 gttaccacat ttactgttaa atattgatcc atggggcacc cctttctagt agcctagtgt
      841 tttcgctttt tctacgacgt cttcgattcg gccaacactt cggaaagctt cttcaggaag
      901 atcatcgtat ttgccttcca agatttcgcg gaatcctttc actgtttctt ctacagggac
      961 atatgaacct ggaagaccag taaattgttc agccacgtta aagttttgtg acaagaagaa
     1021 ttggactctg cgggcacgag aaactaacac tttttcttga tcggataatt catccatacc
     1081 taagatagca atgatatctt gtaattcacg atatcgttgc aatacgtgtt ggacttctgt
     1141 tgctacttta taatgttctt ctcccacgat ttcaggtgct aaagcacttg aagaagaagc
     1201 caatggatct accgctggat aaatcccttg ttctgttaat ttacgttcca agttggttgt
     1261 cgcatccaaa tgggcgaatg ctgttgccgg cgccgggtca gtgtagtcat cggctggcac
     1321 atagattgct tggatcgatg tgatcgaacc ttttttcgta gatgtgatac gttcttgcaa
     1381 ttgacccatt tctgttgcca atgttggttg ataaccaacc gcagaaggca tccgtcctaa
     1441 caaggctgat acttcagatc cagcttgagt gaaacggaaa atgttgtcga taaacaacag
     1501 tacatcttga ccttctacat cacggaaata ttccgcaatc gtcaaaccag tcaaagcaac
     1561 acgcatacgt gcacctggtg gttcgttcat ttgtccgaac accatggctg ttttttcgat
     1621 aactcctgaa tctttcattt cataatacaa gtcattacct tcacgagtac gttcacctac
     1681 accggtaaat acagaaatac caccatgttc ttgggcgata ttatggatca gttcttggat
     1741 cagtacggtt ttaccaacac cggcaccacc gaatagtccg actttcccac cttttaaata
     1801 tggggcaagc aaatcgataa ctttgatccc tgtttccaaa atttctgtac ttgtacttaa
     1861 ttcgtcaaag gctggtgctt ttttatgaat ttcgcttctt tccgcatctt ctgggaaagg
     1921 tgtttctaaa tcgatcgtat ctcctagtac gttgaatacg cgacctaatg tttctttacc
     1981 tacaggaaca gagattgctt tgcctgtatc aatgacttcc attcctcttt gtaaaccatc
     2041 tgttgattcc atcgcaatgg tccggatcac tccgtcccct aattctaacg ctgcttcaag
     2101 aacaactttt gatttatttt cgtcattttt ataaacaact aatgcgttgt tgatatctgg
     2161 taaggattgg tctaaagaaa attccacgtc gacaacggga ccgattactt gaacaatctt
     2221 gcctgaactc atcttttttc ctccaatctc aatactattc tagtgccgaa gctccggcaa
     2281 caatttcggt aatttcttgg gtaatagccc cttgtcttgc acggttgtaa gaaaccgtca
     2341 gctcatcaat gatattagca gcattatcag ttgctgtttt cattgctgtc ataccagcag
     2401 catgttctgc tgttttggcg tcaacgatcg caccgtagat caaactttca gcatactgag
     2461 gcaataactg agacaagatt tcttcttttg aaggttcaaa aatatattct tgttcgaaag
     2521 tagttgcttc ttctggatct aagtctgaga ttggcagcat tttttctaca cgaaactgac
     2581 tagttagcga attgatatga tggttataac atacgtataa ttcatcgaat acttcgtttt
     2641 ggtacatagt tgttgccata tttacgattt tgcggacttc atcaaagctt ggctgatcag
     2701 atagattgcg cagctcatat gctagattga taccgcgtgc tttaaagaag tctgcaccag
     2761 ttccgccaat tgcgatcatc acgtaatcat cgggtgattg gtggtcttct tctaaaatag
     2821 acatcgtttg tttcaatatc gagctgttat atccaccgac taaaccgcca tctgcagtga
     2881 tgacgatata acccgttttc ttaacaggac ggctgagcaa catgctgttg taattgatat
     2941 cccctctagg attttctgag gcaatatcat ttaactgagt agcagtcaaa tgcgtaacga
     3001 tttctcttac ttttgttgca taaacctgaa acttctgtga agaagcttca gattttgtca
     3061 acttagaagc agacaccatc tgcattgccc gcgtgatttg gctcgttttt ttcgttgaag
     3121 cgatacgtgt cttgatttca tttaatgaag cacccattca tctcacctca ctatgcattt
     3181 tgaatggatt tcaatttatc ttctgtagag gaattatttc cttttgtagc taagaacatg
     3241 tctttatatt cctgaatcgc actgttcaac gcttcttctt ctggaagatc ttttgttgta
     3301 cggattgttt caaagatatc tgaatgattg gtatccagat attcgaataa ttcatgttcg
     3361 aagtctaaaa tactgtctac agggatacta tctaagaatc catgtgtcaa agcatacaaa
     3421 atcactactt gtttttcaac agctagcggt gcatgcaatt tttgtttcaa gatttctact
     3481 gtacggcgtc cacgatttag ttttgcttgt gttgctgcat ccaagtcaga accgaattgt
     3541 gtaaatgctt ctaattcacg gtaacttgct aagtctagac gtaaagtccc agcaactttt
     3601 ttcattgctt tgatttgcgc agaaccccct acacgagata cagatagtcc ggcatcaacc
     3661 gctggacgag tacctgcgta gaacaagtcg ctttctaaga agatttgtcc atcagtgatc
     3721 gaaataacgt ttgttgggat ataagcagag atatctccag cttgtgtttc aacaaatggt
     3781 aaggcagtca ttgaaccgcc acctaaatca tcacttaatt ttgctgcacg ttccaataag
     3841 cgtgagtgta agtagaaaac atcccctgga taagcttcac gacctggtgg acggcgaagc
     3901 agcaaggaaa gttcacgata agcaacagct tgttttgaaa gatcatcaaa aatgatcaag
     3961 acatgtttcc cgttgtacat gaactcttca cccattgcag taccagcata tggtgcgata
     4021 taaagcaacg gcgctggttg agacgcaccg gcattcacaa cgattgtgta atccattgcg
     4081 ccatattttt tcaatgtttc aacttgtgta cgaactgtag aatctttttg tccgattgct
     4141 acatagatac agatcatatc ttgacctttt tggttgatga tcgtatcgat cgcaatggaa
     4201 gttttccctg ttttacggtc accgataact aattcacgtt ggccgcgtcc gattggtaca
     4261 agagcgtcga ttgcttttag acctgtttgc attggttcgt taacagattt acgttgcata
     4321 acgcctggtg ccattgcttc tactggacga gctttgtcag taacgatttc acctagtccg
     4381 tcaattggct gacctagcgg attaactacc cgtccaatca aggcctctcc aactggaact
     4441 tccatgatct ttcctgttcg ttttacttta tctccttcac gaatggactc gaaatcgcca
     4501 aggataatga tacctacatc atttgattct aagttttgcg ccattccgta tgagccattt
     4561 gaaaattcaa gcaactcacc actcatcgcg ttttctaaac catgtgcacg agcgatcccg
     4621 tcacctacat aagttacggt accgatttct tcgactgaaa gcacgttttg ataattttca
     4681 atttgttctt taatcaaggc actaatttct tctgctttga tggccattcg attcacctac
     4741 ctctttgtac tatctattta attgattacg catattttct aattgcgtgc gaatgcttcc
     4801 gtcgattact cgatggttag cttcaacaat cgcgccacca ataatagaag aatcaacgat
     4861 ttgttttaat tcgactgatt gatagttcat tgtttttgca acattctttt ctaattgtat
     4921 tcgttgttcg tctgaaagtg gaactgctgt tgtgacactt cccaacagca aaccttgatg
     4981 ttcatcatat cgacgttcat attcttcgat catgaacaaa agatcatcca ttcggttata
     5041 cgcataaaca acttctaaaa agtttttaac gattccatca tagccactaa caagcttgtc
     5101 catgatttct cgtttttcat gaggttctag acgaacatca cttaacatat tacctaatcc
     5161 aggtacttgt tcatagattt gacgtaagct tacgagatct tgataaattt cttctgcttt
     5221 atttgaatcg atcgccagtt caaataaagc tttcccgtaa cgtcttccta ctgtatattt
     5281 atctagtttc atttgaagaa cctagacctt cgatatattg attgattaat gattcgtgca
     5341 tttctggaga taattctttg ttcaagattt tttccgcgat ttgaagagat agttctgcaa
     5401 catcgtcttt tacagagttc agcgctgaat cacgttctac agtgatatca gcttgggctt
     5461 tactttttag acgagctgct tcttcttgag catccttcaa aatattttgg cggcttaatt
     5521 ctccactttc tttcgcattt ttgatgatat cagctgcatc agaacgagag gctaacaatt
     5581 gttgttcgcg ttcttgttcc attttcgctg agttgatgcg agattgttct gcagaatcca
     5641 aatcattggc gatcttgtct tcacgttttt tcaaaatatc gctgattggt ccccaagcaa
     5701 aatgcttcaa gagagccagt agtatcaaaa atgagccgct gacaacgatg acatttccta
     5761 acatcgcatt gccaacttct gcaattgcca attgattcag cataggcgag ccttcctttc
     5821 tttgaagtac ttctagtttt catgtaaaaa agcagtaaca aaccaaattg tttctgcctt
     5881 aatactgatt caattaaaca ccaaaaacta gaatcaaggc gacaactacc cctaagatag
     5941 gaacagcttc gactagagct accccgataa acattgttgt acggaattga ccagacattt
     6001 caggttggcg agccattgat tcgattgttt tagagataac ttgaccgtta ccataaccgg
     6061 ctccgattgc tgctcccatg attgcgattg ctgctgcgat ataattcata attgttttcc
     6121 tcctaaatta gataaatagt agtttgtttt taaaacgaat attattcttc cacttcaatc
     6181 ttatggctca tataaaccat agataaagta acgaagacaa atgcttggat gcttccgata
     6241 aacaaggaga atgcgatcca gaccatctct aatggaattg ctaacggcaa tgagaaccag
     6301 cctaagctta ttaccatttt tgcaatcaag gttagcaaaa cttcccccgc ataaatattc
     6361 ccataaagac gtaaagcaag tgttaataag tttgtaaatt cttcaatcag tttcatcgga
     6421 aacataaaac tatatggttt caagaacgaa tttacgaaat atcctttgaa gcctaacttt
     6481 ttcaccccaa ataagtgagt aagtaagatc attataaatg ccaaagttaa agtaacaaac
     6541 gggtctgctg tcgggctttt ccaaagtgtt gtttcgttag gtaaaacgat ttttgttact
     6601 aaaccgatgt tgtttgcaac aaaaacaaat aaaaacaatg taaatgccaa caaatggaaa
     6661 ttgctcaatt cttttctagg cagattatct gttacgattc ctcgactaaa atcaatgacc
     6721 cattctatcg cattttgttt gcctgttggt ttcaacttaa gatttcttgt aaaaaagtaa
     6781 gctaagaaga aaacaatcaa acaggtcagt aacaccatta tacaaactgt accatcaaac
     6841 caaattggtc caatgtggaa cgttagcgag cgttcatcca atccatttca cctcttttcc
     6901 ccgaaattca cataaaatca attcatgtgc gtatctggtt ttttccaatc ggtttctaac
     6961 cacacggtat cataccacca tgaaacagaa aattcaaaac attttcatga aaattagaca
     7021 cttttttatt tcagtcaaaa acgaagcgaa tttttcttta tacacggtgc tcctgatcta
     7081 agaaaaaacc tatgataatc atgttttatc agattaaatg ctaacaaatt ttatcattaa
     7141 gtaaaagcaa aagcaccgaa tatgcgaaaa aggataatga aattatctag agaaattttt
     7201 taatgttcga tcaatttgtc gatcgatatc ttttcgtttc aaatcttccc gtttatcata
     7261 agattttttc cctttcgcca gaccaatcaa taccttggca tagccgttac gaatatagac
     7321 ttttaaagga acaacagtga ttccagtatt tttcaattca ttttcaagcc ggatgatttg
     7381 ttttttgtgt aataataact ttctcgtgcg caacggatca tgattaaaaa tatttccttg
     7441 ttcataagga ctgatatgaa cattatgcaa gaaagcttct ccgttgcgga cgcgaataaa
     7501 tccatctttt agattgatcc ggctgtttcg tatcgacttg atctcggttc cttgcaagac
     7561 catccctgct tccatcgtgt cgatgatcga ataatcatgg cgagctttct tgttttgtgc
     7621 aattaatttt ccctcgcctt ttggcatagc tgtttcctcc aactcatttt attttttctt
     7681 tttcttcttt ttaacacctt tgtaaaatgg cttcttccct tttttcttac cgaacgtttg
     7741 cttactatcg ctatatcgtt tgttcttctt gccatttctt tctgggcgtt ttcctgcttg
     7801 ttttccttcg cgttttcctg attttccatt tcgtgttttt ggccgttcca acgctgtaac
     7861 ctcttctgcg gaaacaagtt caaaatctac tgctcgttca tctggatcag ctttgactac
     7921 ttttactcgc actttctgtc cgattttaaa agtcagacca gttctttcac ctacaagagc
     7981 taaatgattt tcaacaaaat ggaagtagtc ctgtttcaat tcattgatat ggatcaatcc
     8041 ctctactgta ttcggcagtt cgataaacag gccgaatttc acaacagagc tgatgatccc
     8101 atcgaattct tcccccactt tgtccatcat gaattctgct ttcttcatgg aatctacttc
     8161 acgttctgtt tctactgcac gacgttccat cttagaacta tgatctgcaa tttcaggaag
     8221 ttccgtagcc cacgaacttt gattgctttc acttttatct tcactgtaag aacggatcag
     8281 acgatggacg attagatcgg gatagcgtcg aataggagat gtaaagtgtg tgtagtaatc
     8341 tgccgctaag ccataatgcc caaaattgtc ttctgaatag cgtgcttgtt gcatgcttct
     8401 tagcaacata gtattgataa ctgcagcttc tggtttgtct tctactcctt cgatcacatg
     8461 ctgtaaatct tttggtgtga tcgttccttt cgttcctcgg accagaatgc ccaaagctga
     8521 agcaaaatca aagaagcgct gcattttttc ttcttttggc tgttcatgga ttcggtaaat
     8581 aaacggcaga tttaatttat tataatgttc agctaccgtt tcattggccg caagcatgaa
     8641 agattcgatc aagcgttcac caattcctct gcttcttagc tggatatcat ttgggtgtcc
     8701 ttcagcatca acaaggattt ttgcttcgcg atcctcaaaa gaaatcgcgc cacgtttgat
     8761 tcgcatcgtt tcaaggattc gatgcaattc acccatttct tggaacatag gtacgagctg
     8821 agcatagcgt tgcatcgttt cttcgtcttg atcttctaag atttgattga cagctgtgta
     8881 ggtcatccgt tctgttgttt ggatcacact tgggaaaata tcatgagaga ctacttgtcc
     8941 atttggatca atctccattt cgcaactcat ggtcagcctt ggaacatggg gattcagtga
     9001 acagatacca ttcgataaac gttgcgggat catcggaatc actcgatcag tcaaatatac
     9061 acttgtccct ctttcatatg cttctctgtc caattgactg ccttgtgtca catagtaaga
     9121 aacatctgca atgtgtactc caagaaaata attaccgtta tctaattttt taaccgtaac
     9181 tgcatcatct agatcttttg catcttctcc atcaatcgta acaatgagct gatttcttaa
     9241 atctctacgt ccagttatat ccttttcgct gatttgatct ggaacttcat ctgcttcaga
     9301 caatacatca tcaggaaatt tcgtagggat accattagcc acgacaattg agaggatatc
     9361 catgccaggg tcattcttat gaccaatcac ttgcttgatt atcccttcta aactagtcgc
     9421 ataccctttt tcagggtaat gggtcagttc gatcagcaca atacttccgt caactggacg
     9481 aactccctct gctgctgcat atactttaaa ttggttgagc tttctatctt tcgggataac
     9541 aacgccgtaa agatccgttt cttgtatttc ttcttctgaa taagcaacga attcccctgc
     9601 taactgagta gtcgcccggt ctcgaatctc gactactttc ccctctgctc cgcgatccat
     9661 atctgtattt gctcgcttca tgatatcgat tgctacggtg tctccatcca ttgcaaaacc
     9721 agtcgcttct tttgggatat agatgtcatc ttcttcggga tcaatcgtta caaatccaaa
     9781 tcctcgttca tttgctcgaa atgttccttc gatcaagatt ggttttaaag gaagtttcac
     9841 ttttcctttt ttattgaaaa ctatactctg ttctctttcc atttgagcaa tcgtttgtac
     9901 tagtaattta aaatcatcgc ttttttggaa ccctaaacct tcagcgattt cttccataga
     9961 aaaactgcgt ttgcgactgc tttccatgaa aaagagaatt ttctctttta tcgtttcttt
    10021 tgtcatattt attcctcatt ccaaggtaat ttttctaaaa attccaagac gtctttttct
    10081 aattcgcgtc ttgctgtacc aactgtaatc acatgaccac tttcaggata ccatcgaagt
    10141 gtcactcgct gacggctcaa cttgcgtgca gtttcaaata cgccattagg gtcgatcatt
    10201 tcatctttcc cagcttgagc taaaaatatc ggctgttgga tatctgacaa cctactttct
    10261 acgattgctg cctgatcttg tatatccatt aattgttgtt cagctaacga gcggtaagca
    10321 actgcttttt cattgatttc tttctctgat ttacctgctg tcttcagcac ttgacgtacg
    10381 tatttttcaa agttttcagg cacgtttgtt tttacaggag acaaaggcga acagaaaaaa
    10441 ccgccgccaa tcactgattc ttcttctagt gctcgtacag caaagatgcc gcccattgat
    10501 aatcctaata cggcaatatc agaaaactgc tcagactgta aaaaatggat ggctttttta
    10561 gtgtctgccc accagctttc tgctttttct tgcaaaatat catatggctc cattgttccg
    10621 tgtcctttaa aaagcggcgc ataaactgta taatctgctt tttccaaaaa tcgtgcgagc
    10681 attcgaacat cgtttggact tcctgagtaa gcgtgaagca gcaaaaccgc tcgctttcca
    10741 tgttttgcat aaaggggttt tggtaattgt ctcactcgtc tcacctcttt cgcctcattt
    10801 taccatagtt tctttcataa cttcttctta agcgttctct ttgcaataaa aaaagccgcc
    10861 cgccgttttg cgagaggctt attcaactac ttcgaagata aatatgctag gacaaataat
    10921 aagatcatcc agatagctcc cataactgca gttgatcgct gcatgacagc ttcaaagccg
    10981 cgagcttttt gtttgccgaa tagttgatcc gcaccgccgg taaatgcact cgctgcacta
    11041 ttttgcttgc ttggctgcat cataatcgca atgaccataa caatcgataa aacgatcacg
    11101 attcccaaaa ttaaattata catatcgcag cctccttccc aaaagatatc gtctctttaa
    11161 ctttatcata gataaaagtc cgtgacaact ctatataaat ggaataacag ctgaaagttc
    11221 aaaactttca gctgttattc ttatgactga cgtaagacgt cttgatattc tggtttcttt
    11281 tcaaactctt tttttgcata agggcataaa ggaatgatct ttttcccgtc tcgtcttgct
    11341 aattctactc cagtatacac tagcttttca gctaactttt gaccacgata tgctggatct
    11401 acaaatgtat ggtcaatgat catgatgtct gggcccgcat ctgaccatgt catttctccg
    11461 atctcttgtt gttcatcatt gaataaagca agacgatttt tttcttcttt gatttccata
    11521 tgccgcactc ctcaaataac tgatattttt ttattcctac cttattttag cagtctcctg
    11581 atctataaac aattatttat cctcgaatta cttacaaaaa agaagagaaa attaattcat
    11641 cgttattctt tgttcaataa atgaaaaaat cactgtccaa taggttttcg gatctttcat
    11701 cgatgcttcc aagtgttctg cttctggaac aagcagcagt gctttatccc ctgccgtagc
    11761 attttgaatc gtgtacgctg cttctgtagg gacaaaatga tctctttcac catgaatgac
    11821 caataaaggc agatggttgc tgccaagctg tctcgtagct gatgcttgct ttaaggagta
    11881 accggcgtat ttctgggcat aatgattcgc atatctcatg accatttttt taggaaagac
    11941 agtgatttta cttaacatat atcggaattc tgcataaaca gaaacatatc cgctgtctaa
    12001 aataaatcct ttgaccgctg atggaagttt ttcaccactt gccatcatga tcgtagaagc
    12061 tcccattgat ccgccgtgta aaataatgga cgcattcggc tgttcatcta agatgagttg
    12121 gatccaggca attaaatcta gccgatctag ccatcccatt ccgatgattt ctccttcact
    12181 ttctccgtgc gcacgtaaat caggtacaag aacgttgaat cctttctctg catatctttt
    12241 acctatatga gacatgtctc ttttccctgt gcttcggtaa tcatgtacgc aaatgaccca
    12301 tttattctgc ttatttggat ggatgaacat ctgaccagct agtttcaacc cgtcataact
    12361 tttgattgtt ttgggtgttc cctgctccca aaagctttca cactcttctt ctaatttttg
    12421 tcgttttttt tctactgacg tgactgcagc agggtccatc aaacgatgac cagtgtcttc
    12481 ataccagtca tatcctctct gtactcccgc tcttactaat ttctttgcat aaaaataaga
    12541 aagaaaatgg taactgatcc ctataattat cagaataaga atgaaataaa aaataaccat
    12601 tgtacacccg ccgctttctg ccaaactata atctaactat tatccaatgt accgatccat
    12661 aggattcttt attttcggca cacgtaaatt ccatttcttt ttcattgtat catttactta
    12721 aagttctcct caatagattt acttgtaaga gagcatttac attcgcaata aaagccagta
    12781 tactaatttc gtgcattata atcaagacgc tcgtcttgga ctggttcgga aacttcccag
    12841 aataaaaaac taagtatctc taaagaaagg agattggaaa taatgcgaaa agtaattatt
    12901 gattgcgatc cagggatcga cgacacttta gctttatcat tagctgtcaa atcccctgat
    12961 atcgaagtag ttgctattac cgtagtttgt gggaatgtcc ctgcagatat aggtacgcag
    13021 aatgtgctga aatgcctgga acgctgtgat cgtttagaca ttcctgttta tcaaggaatg
    13081 gaaaaacctt tgaagcaacc tttcatcagt gcacaagaca ctcatggtat ggatggttta
    13141 ggggaaacaa atttcccgat gatcttgaga aaacaggcag aacctctcca tgccgttgat
    13201 tttttggctg attatttcaa agaaaagaca aatacatcag tcattgcttt gggtcctttg
    13261 accaatatcg cttttgcttt gaaagtcaac ccaaatattg ggaaacatat ggatcgtttt
    13321 gtctcgatgg gtggaactta caaaagccat ggtaactgtt cgccagttgc tgaatacaat
    13381 tactggtgtg atcctgaagc agcttcttat gtattcgaga atctcaaaca aacgatagaa
    13441 atggttgggt tagatgtgac aagagaaatc gtcttgacac cgactatcct cgaatactgc
    13501 tgtcaaatga atcctgaaga gggagagtat ctaaaagcga tcactcgttt ttattttgac
    13561 ttccactgga aacaagaaag aattttagga tgtgtcatca atgatccgtt agctgttgct
    13621 tattttatcg agggggccat ctgtgaaggt ttcaaaagct tcactgctgt ggaaactcaa
    13681 ggaataagtc gtggacaaac attggttgat cgttatgaat tttggcagaa acctgctaac
    13741 agtaaaatta tgacaaaagt agatactcgt ctttttttcc gaaagttttt aaccgttctt
    13801 ttaaatgcac aagaagaaac gattatgaaa gatttggaaa ggttaaagat gggctaacaa
    13861 aatgaataaa aacaaaatgg caactcgcgt acttacagtg atggctttgt gtattgggat
    13921 caattatatt ggaggaacga ttgctttatg gcttcgtttg ccaatctatc tcgattctat
    13981 cggaacgatt tttgctggtg cattgcttgg gcctgtacct ggaatgctga caggattgtc
    14041 cagcggtatt ttaagtggag tgactacaga tattttttct ctgtattatt cgccaatcca
    14101 aattctcaca ggcttgcttg ctggattgat tttgccaaga tatctcggca aagataaaaa
    14161 cagcttgaaa ctgttattac tcgctttgat tttgtcggta cctggaacga tcgtttcttc
    14221 gatcattacc gttcagttat ttggcgggat cacctcctca ggatccagta tgatcgttca
    14281 gctattacat gggttaggtt taagtcagac aacaagtgtg atcttggtac aggcaggtac
    14341 agactacctt gatcgtttgt tgtctatcgt cgttgtttca ttagtcatca ctatgttgcc
    14401 acaacgcttg atatattcaa aaagaaaaat aggctgaata aacagagctg gattagcaaa
    14461 ataatgctaa tctggctctg ttttttcatt tattaaaaaa atcttacgct aagtaggata
    14521 aaataattcc ttgatatttt tcaatcatat ccaaataatt atcgatttca acatactcat
    14581 ctacttgatg aggcaaagta acgacaccag gcccaaagac gacaaagtcg aaggaatggt
    14641 tagctttcgt aaattcggct gcatccgttg ttgctgccgc tccaactaaa ggtaatggct
    14701 ggctgaattg ttgctgaatg caatgaatca aaggagaatc tggatcagct ttgacaggta
    14761 ttttgttgta atcgatcatc aattccaaat gatagtctgt ctcttgattc aattcattga
    14821 cgattgattg cagcaatgca atgattttat catttgggta ttctggaatc gatcgaatgt
    14881 tcccttggag ctgcgcatgg cttgggatgc tgttgacttg attcccgcca ctgatcaatg
    14941 taacattatg gattgttttt cctaggacag gattctcgat tgtttcagcg aggtgattca
    15001 tttcagcatt tgctttcgta atgaattcat ttaggtgatt gattgcatta taaccttggt
    15061 ctggcataga gctatgcgct tcttttccgt gcgaggtgac tgtatagttg attgatccca
    15121 tgtgcgtata catcaaactg taattagttg gctctccgat gatcaaggca tctaagtcat
    15181 ctacatagcc tgctttcgtg agctgttccc cacctagttc acccacttct tctccgacag
    15241 tggctaataa tttgactgtt ccgttaaatg gttttcctga ctctttcagt tcgatcatgg
    15301 caatcaccat tgctgccaag ccactcttca tatctgtagc accacgtccg tacaaacggt
    15361 tcccatggat ttctgctgca aagggtgcat aggtccaaga cgattcatca ccagccgcca
    15421 cgacatccat atgcccagat aggcctaaca cttttcctga ttggcccttt tggtaacgag
    15481 caatcaaatt atctctacca tctctgtaag aaacaatctc tcctgttatg tcatgctttg
    15541 ccaaaagctt attcaaatag gctgctactt caccttcgtt tccattcact gatttaattc
    15601 ggatgatctc ttgtaaaatc gcgatttttt cttctttttt catctaatcc tctcccttgc
    15661 catttctttt tattgtattt gagtgtgctc gaattttcaa ataggagcaa ccaaagtaaa
    15721 aatgaagaaa gagagccact ttatgaaaaa tatgctgcta cttccctcct attttattta
    15781 tacgagagga cttgctcata gattcctatt ctactgttct atccttaaaa gctcttatat
    15841 gatgctagtt attgttagct ttcctttttc aaggatgatc ttttcttgta ccattctttg
    15901 taaacatcat atacagaagc tagttgcaaa aaaaactctc tacctgctga tatt
//
LOCUS       DAYYCY010000056        15918 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000056 DAYYCY010000000
VERSION     DAYYCY010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15918
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.056"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(59..187)
                     /locus_tag="ACHAZB_001391"
     CDS             complement(59..187)
                     /locus_tag="ACHAZB_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFM8614076.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="ACHAZB_001392"
     CDS             complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="ACHAZB_001392"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFM8614077.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(1674..2729)
                     /locus_tag="ACHAZB_001393"
     CDS             complement(1674..2729)
                     /locus_tag="ACHAZB_001393"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFM8614078.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="ACHAZB_001394"
     CDS             complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="ACHAZB_001394"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HFM8614079.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="ACHAZB_001395"
     CDS             complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="ACHAZB_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HFM8614080.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="ACHAZB_001396"
     CDS             complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="ACHAZB_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HFM8614081.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="ACHAZB_001397"
     CDS             complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="ACHAZB_001397"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFM8614082.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="ACHAZB_001398"
     CDS             complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="ACHAZB_001398"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HFM8614083.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            10491..12050
                     /locus_tag="ACHAZB_001399"
     CDS             10491..12050
                     /locus_tag="ACHAZB_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HFM8614084.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(12161..14092)
                     /locus_tag="ACHAZB_001400"
     CDS             complement(12161..14092)
                     /locus_tag="ACHAZB_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFM8614085.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="ACHAZB_001401"
     CDS             complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="ACHAZB_001401"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFM8614086.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(15021..15773)
                     /locus_tag="ACHAZB_001402"
     CDS             complement(15021..15773)
                     /locus_tag="ACHAZB_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM8614087.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
BASE COUNT         4107 a         3488 c         2910 g         5413 t
ORIGIN      
        1 taaaaagact aagaaacaat aacaaaagtt aacacttctt agtctttctt tagcacgtct
       61 atttattaaa gattttaggg ccttttctca tgtttttacg atcattctta ggtgtttcca
      121 tttgtttgga tcggcctcca ccttgtttct ttttgatcaa ttctttcatt ttttcttttt
      181 ggtccatttt tattctcctt taacgaatga agcgactaat tcgatatgat gggtttgcgg
      241 gaaaagatct acaggctgaa ctttcttcac ggcataacct gcttctgcaa ataacttagc
      301 atctcttgca aaagttgccg gattacatga gatatagacg attcgttctg gtgacatttc
      361 gattgctgat tcaataaaat gatgatctaa tccttttcgt ggagggtcaa ctacgatcac
      421 agatggtttg atcccttttt cttgccattt tttcataact ttttctgctt ttccgacttc
      481 ataatgaaca ttttcgattt tattatttaa agcattgaac tgtgcatctt caattgcttc
      541 tggaataact tccatcccat acaccttatc cactctgtct gctaaagaca agccaatcgt
      601 acctattcct gaataagcgt cgatcacaat gtctttttta tctaactcgg caaactcgat
      661 ggctgtttgg tacagatact ctgcttgttg agtatttact tgataaaatg actttgctga
      721 gattcgatag gtttttccta atagctgatc ttcgatatag tctctgccat ataaaacttt
      781 ttctttgtct cctaaaatca catttgtttt ttcttcattg atattttgga tgaccgaaac
      841 cacttctggc aattcttcat gaatagcttc agcaatttcc ttggatttaa agaatttttc
      901 tttacgagta actaaaacga ccatcatttc atgggaataa tgaccacgac ggataatgat
      961 gtgacgaagc tgtccttcat ttttttcttc attatatcct cgtacttgga accgttgcaa
     1021 gatatctctg actactttga ttgcttgatc gatgacagga tcttggatat aaaaatcttc
     1081 gatagggact agatcatggc tattcttttt gtaaaaaccg gtggctaact gtccatcgat
     1141 acggcgaaca gggatctgtg ctttattacg gtagccgaat ggttctgcca ttccgaccgt
     1201 tggaagtaca ggaacttctg gcatcttagc aatcttcgcc attacatgct ctacttgttt
     1261 ttgtttgaag acaagctgtt gttcatactt caaatgggct aaaggtgcga tacctatgcg
     1321 aagtaatgca ttgttttcta caggctgacg atcaggtgat tgtgtaataa atgtttcgac
     1381 ttttccatat ccaaacttat tgcctgcttt tactactagt atttctactt cttctccagg
     1441 gagtgcattt tcgataaata atggataacc atcgattttt gctaccccca ttccttcata
     1501 actcaagtcg acgatttcga ctgtatatcg ttcgtttttc tttactattt gctgttctgt
     1561 catattttgc tcctttaagc ctatttttca atcctactga actctactat aaatcagcta
     1621 attacacaac taaaaccggc actgacctta ctacgttcag caccggcttt tgattaattc
     1681 ttttgattag gtgtttcgtc tgctattttt ccgtcaccgt cgatatcttc atccgttaaa
     1741 cgttcgactt cttttacgaa ttctttgctg acaacctcta attcttcttc atctgtacct
     1801 gtgattgctt catcaggaat ctgatcggta ttggcaaaga actcaatgtg ttgatgaaga
     1861 ttttggaaat gcatcggtgc ttccccgccg tattcaccat ctaagttgat cattactggt
     1921 cgattgatat catcttctgt ttcaacgaac aaactgcttg ttttggtata gattattctt
     1981 ggatcatcga catgtcgacc gccattcagc atgagtgctg caatatgcaa gatctcaaag
     2041 atatttgctg tcttcacaat gattaaagaa aacttcccat catccaattt ggcatctggc
     2101 gcgatctgtt cgaatcctcc aacagagttc gtcagcccta ggaaaaacat cgaagcattt
     2161 ccgatatatt ccccttcatc atacttcaag cgcattttga ttggctttac tcgagggagc
     2221 atctctgctc ctttcgcaag ataagcaaga taaccaaaaa tactctttag ttctgatgga
     2281 acttcataag tcaattctgt caaatagcca cctgcagcga tattgatgaa gtaactgtta
     2341 tcagaaaggc cgatgtccat ttttacggtt tggtgttttt ttatcacttc tgctgctgct
     2401 ttaatattat ctctaggaat ttttaatgct cgagcatagt cgttcgttgt accagctggg
     2461 atgatggcca ttttaggacg gtgttcaagc ggagcgattc cattaaccac ttcgttgatt
     2521 gtgccatcgc caccagctgc cactactaga tcaaaaccta gttctgcgac tctacatgct
     2581 tcattcttgg ctgaatctgg ttctggtgta gttgcatagg cgctggcttc aaatcctgct
     2641 tcttctaatg cctgtaggat atccgccaaa ttttttttca ataattcttt tcccgaggtg
     2701 gggttataaa ttacgcgtgc ttttttcatt ttcagcctct atcgtttcgc taattcatct
     2761 ttaagtagct tattcacgac accaggattt gcttgtcctt tagttgcttt catgatttga
     2821 ccgactagga agccaaccgc acgatctttt ccatttttga aatcatcgac agattgttgg
     2881 ttgttatcca aaatttcatt aatgattggc agtagtttgt tcggatcaga taattgcacc
     2941 caaccatttt tttcaactac ttcttgggct tctccgccat ttgtgatcaa ttcacggaag
     3001 acttttttgg cgatttttga gctgatcgtt ccatcggcga tcaaacgaat cattcctgct
     3061 aagttctcag gtgtcaattt tgtctcagct aattccagtt tctcactgtt caagtaagca
     3121 gaaacttcac ccatcaacca gttagatgct tgcttggcat ctgcaccatt ttccaaaaca
     3181 gcttcaaaaa aatcagacat ttcttttgta agtgtcaata ccattgcgtc atactctgga
     3241 agtccaaact cacggatata gcgttcgcga cgagaagctg gcatctcagg caaactagct
     3301 tttacttttt gaatccattc gtcatcgatc acgaatttcg ggatatcagg ctctgggaag
     3361 taacgataat cactagaccc ttctttgaca cgcatcaaaa ctgttttatt cgttgtttcg
     3421 tcaaaacggc gtgtttcttg ttggatttct cctcctgaaa gaagaacttt tgcttgacgt
     3481 ttttcttcaa aagctaaacc ttttttcacg aaagataaag agttcaagtt tttcaattct
     3541 gctttcgtac cgaactcttc ttgtccataa gggcgaagag aaatattggc atcacagcgc
     3601 atagagcctt cttccatttt cacatcgctg acttctgtaa attggataat tgatcgcaat
     3661 gcttctagat aagcgtatgc ttcttctggt gaacgcatat cagcttcaga aacgatttcg
     3721 atcaaaggcg tgccttgtcg gttcaaatca acgtaagaat aaccgccaat accatgcatg
     3781 ttttttccag catcttcttc taaatggaca cgttcgatcc ggattttttt ctttttacct
     3841 tctacttcga tttcgatcca tccgtcatga ccgattggtt gatcaaattg ggaaatttga
     3901 tatgcttttg gattatctgg gtaaaagtaa tttttccggt caaaatgagt atctttagag
     3961 atttcacagt ttaatgccaa agctgctttc atcccgaatt ccagcgcagc tttattcatt
     4021 actggcagca cgcctggata accccagtcg atcacgtttg tattgctgtt tggttctgca
     4081 ccaaaatgag ctggcgctgg agagaaaatt tttgagttgg tttttaactc gacgtggact
     4141 tctagtccaa tgactgtttc aaaattcatt cgtctttccc tcctaaaata gctggttgtt
     4201 gtttatggaa ttctgttgct tgttcaaacg catatgccgc ttgatacatc gttttttcat
     4261 caaaataatt accgatgatc tgcaatccaa ctggtaatcc ttctgataaa cctgccggta
     4321 cagacatacc tggaagtcca gccaagttga cagggatcgt taagatgtca ctcatgtaca
     4381 ttgtgattgg atcgttaatg ttctcaccga ttccgaatgc aactgttggt gaagatggcc
     4441 cgatgatcaa gtcataatct gcaaagactt tttcaaaatc tcgtttgatc aatgtacgga
     4501 cttgtcctgc ttttttgaag taagcgtcat aataacccgc gcttaaggag aatgtaccta
     4561 acatgatacg gcgttttact tcttcaccga atccttctga acgtgagttg acatatacat
     4621 catctaatga tttcacattt tcagagcgat aaccgtagcg gataccatcg aatcgttgca
     4681 aattggaact tgcttctgat gaggcaatga tataataaac agccacaccg tattttgaat
     4741 gaggcaagct gacttcttcc acaacagcac ctaatgaacg gaatgtttct gctgctttca
     4801 atacagcttc ttttacgcca ggggccacac cttcgcctaa gtattcttta ggtaaagcaa
     4861 tcttcattcc tttgatgtcg cctgtcagtc cctctgcaaa atcaggaact gaaacgcctg
     4921 aagaagtacc gtctttttcg tcataaccgc tgatagctgt caatgctaat gcattatctt
     4981 tcaccgtacg ggtcattggt ccgatttggt caagagatga agcaaaggca atcaaaccaa
     5041 aacgtgagac acggccataa gtaggtttca tccctacgat gccgttaaaa gctgctggtt
     5101 ggcggatact tcccccagta tcactaccta aagaaactgg aatttgtcca gctgcaactg
     5161 ctgctgcaga accaccagaa gagccaccag gtacttttgt tgggtcccaa gcattttttg
     5221 ttttcttgaa ataagaagtt tctgtacttc cacccatagc aaattcatcc atgtttagct
     5281 taccaacagg gatcatatct gcttgataga ttttatccat gaccgttgca tcataaattg
     5341 gttcaaaatt gtaaaggatc ttagatgcag cggttgtcaa gatatctttt gttacgatat
     5401 tatccttgat accaattgga ataccagcaa ggacgttgct ttcatcaatc cctttttcat
     5461 ctaaagcttt tgcctgttcc aatgcttttt cttcgttcaa tgtaatgaat gcctcgacct
     5521 caggctctgt ttctttgatt cgttcaaatg ttgtttttgt caaatcttgt acagtgattt
     5581 ctttcgatac aagcatatga tgcagttctt ctagagaacg attgtgtaat tctgtcatta
     5641 tgcgccagcc tccccattat cgatgattgc gggtactttg ataaagccgt cttctgtttc
     5701 cggcacattt ttcaataatt catcgcggtt ccatccagct tgcgcccgat cttgtcgcat
     5761 cacgtttact gtttcaacca cattcgacgt aaatggaaca ccttccgtat ctacttcttc
     5821 cagcatttca accatatcga tgattttacc taattggtcg gtaaaacttg caagttcttc
     5881 atctgcgaac gcaagttttg ctaatttggc aacgtgtttc acttgttctt cactaatcgc
     5941 catactaccc cttctttcta aacgagttaa aaactttgct ctctatttta acacaagagt
     6001 gccgtgtctg aaattgttta ttgaaatttg tctgaaaaaa ctcatttttc attcttctac
     6061 tgagcgactt ttagccaatg catctgccat ttgatcttct gtccaaactt cgattcctaa
     6121 ttcttgtgct ttggtcagct tgctgccagc atcttctcct gctaccacga tatccgtttt
     6181 tttcgatacg ctaccagtca ctttcccgcc aagattttcg atcgtctctt ttgcttcttc
     6241 acgagtaaat cgtgttagtt taccagtaag gactactgtt ttttctttaa aaggagactc
     6301 gacttcttgc aattgggcat ttcgaagccc tttgtattcg aaattgacgc ctgcttgttt
     6361 taattcattc atcaattcat ggacttcttc gttactgaaa taagtgacta cactgtcggc
     6421 aatgatatca ccgatcgtat caagcgcaat aatcgactca tagtcacttt tactcaatgt
     6481 ttctagatca ccgaaatgtt ctgctaaaat ttttgctgct ttcgctccga catgtcggat
     6541 tcctaacccg aaaatcaacc gctcgaccga attatctttg ctgttgtcga ttgctgtcaa
     6601 aatattattt gctgattttt cttttatttt gtccaacgtc aataactctt cttctgtcaa
     6661 tttgtaaaga tcagcaacat ctgctactag ttttttatca tacatctgtt ctagtacgcg
     6721 cggccccaac ccatcgatat tcatcgcatt tcttgaaaca aagtggttca aaccttcttt
     6781 catttgagca gggcatttag gattgatgca ccgtaaggct acttcttcat ccaaatggac
     6841 aagttcactt ccacaaactg gacaatgtgt aggcaattgg tattcttcgc tgtctttagg
     6901 tcgtttatct aacacgactt gcgaaacttc tgggatgatg tctcccgctt tataaatcag
     6961 aactgtatct ttcaagcgga tatccttcaa cttaatataa tcaccgttat gcaggctggc
     7021 acggcttact gtcgttcctg ctactcggac tggtgtcatg atagctgttg gcgttactac
     7081 ccctgttcga ccgactgtcc attcgatgtt ttcgattagc gtttcaactt cttctggcgg
     7141 aaacttgtaa gctgctgccc atctcggtgc tttgatagta aaaccaagtt gatcttgtag
     7201 cgagaattca ttcactttga tgacgattcc atcgatttca tagggcaaat ccacacgttt
     7261 gtcatgatat tcttcaatgt atgaccacac ttcatcaatc gaatgacata gacgtttttc
     7321 ccgattcgtg tggaatccaa ttttttctag ctcttctagc gcatcatact gggtttttgc
     7381 ttgcatagga ccgaaatcag ctactgtgta taaaaaagtg tccagatttc ttttcgctgt
     7441 gattttagaa tctaactgac gcaaagaacc agctgcggcg ttccgaggat tcgcaaagat
     7501 atcttttccc tctgcttctc tgtcttggtt caattggaca aatgaccgtt ttggcatgaa
     7561 acattcgcct cttacttcga tgttcattgg ttcttttaat ttgattggga ccgaccggac
     7621 agtttttagg ttttcagtga tgttttctcc tacagttcca tccccgcgag tcgcacctct
     7681 cacgaaattt ccatcttcat aacgcaaaga aacggaaagt ccatctattt ttagttcaca
     7741 gcaatagtca actacacgtc ccactgcttt ttgtacacgt tgatcaaaag ctatcagctc
     7801 ttctttgctg aaaacgtcgt tcaaactgta aagcggaata tcatgagtga ctttttcaaa
     7861 accttctaat acttttccgc ctactcgttg agtgggagaa tcagacgtga tcaaatcagg
     7921 atattgttcc tcgattgcta ctaattctgc gtattcttta tcataaacat agtcttccac
     7981 tgttggttta tcttctacgt agtattcgcg agaccattgg ttcagtcttg tacgaagttc
     8041 ttctgctcgt ttagcagctt cgtcaatcgt catttcatgt tccatcattt accctccact
     8101 actaagcttt ttcaattggc gcaaaggcag ccaaaagacg ttttactcct tgacttggga
     8161 aagccacatc tagttctaga tccttcgacg tcccgcttac tcggacgact gttcctagac
     8221 cccatttttt gtgtttgact ttatctccag gtttccagct gttattttcc cctccagtag
     8281 cacttttaga gggaacaacc gctgtctctg gctgtttata gctatatgct gtttttttag
     8341 cgaatacagg cgttttttta ggcgtaggac gcatgccttc gatttccagt aactcctgat
     8401 cgatttcttc tacaaaacga gaaggtcggt tatattgcgt ccgtccatat aatgttctgg
     8461 agaaagcatt tgttaaatac aacgcttctt ctgctcgcgt gatccccaca tatgcgagac
     8521 ggcgttcttc ctccagttcg ctttcttcca tcaatgctcg agataaagga aaaaccccct
     8581 cttctagacc aatcaggaat acaactggaa attctaatcc tttggctgca tgcaaagtca
     8641 tcaatgtcac ttgggaagca tcttcttcta aattgtcaat gtcggaaaca agtgccagat
     8701 catttaagaa gaccgttagt ttttcttctg gtgcatcagc gtcttcttca ttttgctgtt
     8761 cgaattgttt gtcgaattct tgtgtgaccg ttaaaaattc ctccaagttt tcgatgcgtg
     8821 cttgtgcttc taatgtattt tggattttta agtcttctag atatccgcta cgatccaaaa
     8881 cctctttcgt caattctgtc actgtcaagt atgggatcat ctgagtgact tcttggatca
     8941 tctctccaaa tgcacctaat tgctgtcctg cttttccacc gatatttgcc agatccacat
     9001 tttgcgctgc ttcaagcagt ggccattcat gaagagaagc aaaactgcgt agtttttcga
     9061 tagatcctgg tccgatgcct cgtttgggcg aattaacgat acgctcaaaa ctgattgaat
     9121 cttgaggatt taccagcaca ttcaaatacg ctaaaatatc tttgatttct ttacgatcgt
     9181 agaacttgtg ccctccaacc attttataag gaatatttgc tttcaacaga gtttcttcca
     9241 ttacacgaga ttgtgcgttc gtccggtaca agatggcaaa gtcgccgtaa ttccgatgat
     9301 tgcttcgaat ctcttcttgc atccggtcta cgataaaacg ggtttcgtct cgttcattat
     9361 ctgcacggta gtaagtgatc ttgtttcctt ctttgttttc tgtccacaag ttttttggtt
     9421 ttcgattgct attgttttca atgacttggt ttgctgcgct aaggatgttt ttcgtggagc
     9481 gatagttttg ttctagcaaa attactgcag catctggata atctttttca aaatccaaga
     9541 tgttctgcat atctgcaccg cgccagccgt aaatactttg atctgcatct cccacgacgc
     9601 acaggttcct aaatctacct gccaataaat tgactagcgt atactgtgcg tgatttgtat
     9661 cttggtactc atctacatga atataatgga atttgttttg ataatacgtg agtgaatcag
     9721 gatgttcttc aaataatcga atcgtattca tgatcaggtc gtcaaagtcc atacattgat
     9781 tatattgaag ctctttttgg taagcagcat aacattttgc cgcaatctct tcgtaataac
     9841 tgccttgcat ttttgcaaaa tcctgagggg tctgcaggct gttttttgct tgagagattg
     9901 ttcctaatat acttcgagga tcatattttt tcggatcaat gttcagttct tttagtatcc
     9961 gtttcatcaa ggtcagctgt tcagaagaat cgatgattgt aaagttccgg ttatacccga
    10021 taaaatcaac atctcttctt aaaatacgca cacacatcga gtgaaaagtc gatacccaga
    10081 catcttcgcc tccagatgcg agaatcgcat tgacacgttc tttcatttct tttgcagcct
    10141 tattggtaaa agtaatcgct aggatgttcc aaggattgac ttctttttcc tcaatcaaat
    10201 aggcaatgcg gtgtgtcagc actctcgtct ttccacttcc tgcacctgcc attagcagta
    10261 atggtccgtc agtgtgaaga acagcttctt tctgacgggg gttcatcccg tttaataatt
    10321 cagctttgga tgacattaac aacatccttt cttttatcaa tcttgttaat tatagcattt
    10381 tcttggcttt cattaaacta gctagttcaa aatcatttgt ttctttttat ggatttttcc
    10441 ttgtcaattc gttgatttcg cgctaaatta aaagaaagcg aggcgtttga atggaaaaaa
    10501 aaggaaaaat caagaaatta gataagactc agatatattt tattctaaaa ggaatatttg
    10561 ttggactgct gacaggaatc gtcgttagcc tgtttcgatt atctatcgaa aagctaagcg
    10621 atttagttcg aagtatctat gaaatgagtc gggaacagcc gatttactta attggcctag
    10681 ctatttgctg catccttgca gcatttttcg tgggttatct ggtcaaaaat gaaccggata
    10741 tcaagggaag cgggattcca caagtagagg gacagcttcg cggggaatta tcaatgaact
    10801 ggttctctgt tttatggaaa aagttcattg gcggtgtact ttcagttggt gcgggccttt
    10861 ttctaggtag agaaggacct tctatacagc ttggcgcatc cattggacaa ggagctggac
    10921 aattgtttcg gagtccatct tcagaagaaa agattttaat ttctagcggt gcaagtagcg
    10981 gacttgcagc agcattcaat gctcctatcg caggattact ctttgtttta gaagaaatcc
    11041 atcactcctt ttctccactt gtttggctga catcatttgc ttcagcaatc actgctaact
    11101 tcgtctctct ttactttttc ggcttgagac ctgttttata tctaggtgac cttccttctt
    11161 tgccgttgaa atattatggt tccttagtga tcttaggagt acttctaggc atcttaggtt
    11221 ttatttatca gaatgtcttg ctggctttgc caaaatttta caaccgattg cctttaccag
    11281 cttattttca cgggttcgtt cctttcttgc tgattttgcc aattggttat ttatggccta
    11341 gtactctagg cggaggcaat caaatcgttt tagcttttgg acatgcttcc ttgccaatca
    11401 ttatgattat cggtttgttt gttcttcgat tcgttttttc catgatttct tacggtgggg
    11461 gtcttccagg ggggattttc cttccgatat tgacattggg ggctttaatc ggtagctttt
    11521 atggaaacat tatggttgat ttcgggatgg atccaatcta catcaaggat tttgtcgtta
    11581 ttgccatggc tggttatttc acagcgattg gaaaagcacc gctgacagca attattttag
    11641 tgacagaaat ggttggctct cttaatcatc tgatgccttt aggattggct gctcttgttg
    11701 cttatatcgt caatgacctt ctcggcggta atccaattta cgaatcatta ttggaacgtc
    11761 tacttggaaa tactcagacc gcctcactaa aaggtgcaag gacaactttt gattttccag
    11821 taacggcaga gagttcttta gacggattaa tggtacgaga cttcaattgg cctaaaaata
    11881 tgctgctaat ctctatacgc agaggaaatc aggaaatctt gactcacggt gatacgatca
    11941 tgaatgtagg agatattttg gttatcttga ctgatgaagc aaacttgcca agaatcaagc
    12001 aggaaatcga ggctaaatct tctatcacaa atttgaagca atcattttaa cgaacatgga
    12061 aaaactattt tacaacaact attcatctac actacttgat tctatatgat tctataaaaa
    12121 aagaagaggc tgggaaaacc cagcctcttc ttttttattt ctatgcttct gctactgcag
    12181 gagcttttct caataaacca taaatcacac cagatacgac tgctccgata gcaataaaca
    12241 ccaaatacat gaccgggtga ctcagtaaga atacaacgaa aatcccgccg tgaggagcca
    12301 gtaatttgat tccgaaagct ccgactaatc cgccagttaa agcagaacca ataataaaac
    12361 ttggaatggc acgaatcggg tcagctgctg caaatggaat cgatccttcc gtgacaaacg
    12421 atagtcccat cacgatgttc gtcaaaccag catcttgatc tgttttgctg aatttattct
    12481 taaacaaacg tgtagcaacg aaaattgcca atggaggtac cattcctcca gccataacag
    12541 aagccatcac gacacttccg ccagttgcaa cagtcgtagc taaagttgct gtaccaaaca
    12601 cataagcggc tttgttgatt ggaccgccta aatctgccgc catcattcca gctaataatg
    12661 caccaagtaa agcagcattc gaaccactca tacttgtcaa gaaaccgttc aatgcatcat
    12721 tgattgcttt catagggaca ttgaccagca acatcaagaa accagtaatc agtaagccaa
    12781 acactggata aaagaggatc gttttaattc catccaatga ttttggtaag ttcttgaata
    12841 atttacgtaa aaatacgatg acataaccag ctaagaatcc accaatcagt gcacctaaga
    12901 atcctgcacc gcctgtatta gctaatgcac cagcagcaaa tccaacgatc aaaccagggc
    12961 ggtcaccgat acttgaagca ataaatcctg ctaataccgg cagcatgaaa ccaaatgcag
    13021 cttgtccgat ttggttgaac cagctagcag cttgattgta attccctaga tttgataatt
    13081 gatcatgtgg cacgccaata aattggtcga tcatgaaaga aagcgcaata gcgattcctc
    13141 caccaataac gaaaggtaac atgtgtgata cgccgttcat caaatctttg taaattcttt
    13201 gtccgatagt accatccgca tttccttctt ctgaactgct ttcttttcca tcaccatgga
    13261 atactggtgc agtaccacta agggctagat tgatcaactc ttctggttta cggataccat
    13321 cactgacagg acgattgatc agttcttttc cgtcaaagcg attcatctct acttttttgt
    13381 ctgctgcaac gattacacct tctgcacggg cgatgtcttc agcagtcaat cggtttttga
    13441 ttccttctga accgttcgtt tcgactttga tctccacacc catttctttc gcttttttct
    13501 tcaaagcatc ttctgccatg taagtgtgag cgatccctgt tggacaagct gtaacggcaa
    13561 caacgaattt tctttctgga tgagcagaag cttctgcagc ttgtgtttct tgttctgctt
    13621 tttcttctgc ttctttttgc tcttcagctg tttggaacaa gtcttggact tcttctggtg
    13681 tatttgcttc ttttaatttt cctacgaaat ctgcatcaat cagcaatcga gatagtgctg
    13741 ctaatgcttg caaatgtgtg tcgtttgcgc cttctggagc tgcgatcatg aaaaacagat
    13801 aagttggctg tccgtctaag gcttcataat ctactccttt ttggcttttt gcgaataaaa
    13861 ctgttgcttc tttaactgca ctgtttttcg cgtgaggcat ggcgattcca tcaccaagac
    13921 ctgtagaagt ttgtgcttcg cgggctagaa ttccttcttt atatgtttcg atatcggaaa
    13981 tccggccggc atcaaacatt ttttggacca tttcatcgat tgcgcctttt ttatcagtcg
    14041 cttgcaaatc catgatcatc gcttctttga ccattaaatc cttgatattc atgattatcc
    14101 tccttgaaac taatcaattt ttgtgatttc aacttctggc agcaactcat caatcaattc
    14161 gcgagctgcc aagtcatcag aaaaagctgt tgcgcttccg caagcaactc cccatttaaa
    14221 ggcttcgatt ggatctttcg tacggctgta agccccaata aagccagcaa tcattgaatc
    14281 ccctgcacct actgaatttt tcaatgggcg aactaataca ttcgttttat aagttccttg
    14341 ttccgtgaag agcaaagcac cgtctccagc cattgagatc agcacattct gtgccccttc
    14401 agctagtaat tttttcccat acggaatgat ttcttctaca gattggaagg ttgtgttgta
    14461 aagctcagct aattcgtgat tgtttggttt gatcaacaac ggtttcttaa caagtgcatt
    14521 ttttaggtca tcacctgttg tatcaatcac gaaatctgca cctttttctc gaatgatatc
    14581 aatcagttct tgataaaatc cttttcgcag gctagctggc gcgcttcctg acaatacgac
    14641 gatatctcct tgtcctactg ttcctaatgc ttgcttcaac tcatcgattt cttcatctga
    14701 aatagatggg cctaagccat tgatctcagt ttcagtgtct gattttagtt tgatattgat
    14761 tcttgtatct tcttttacgg ttgtgaacaa agactggatt tgttcttttt caagccactt
    14821 cgaaataaat tcgccagtga accctcctaa aaaccctagt gctgtagatt gagaatcgat
    14881 ccgtttcaaa atacgggaaa cattgatccc ttttcctcca ggtaacttga agtcttcctt
    14941 cattcggttc aaatcgccta gttttaaccc atctacgcgt acgatataat caattgatgg
    15001 attaagtgtt actgtgtaaa tcattgtatt gcctccttaa ttgttgtctt ttcacggaat
    15061 tcattaacga attctaatgg acaacgctcg gttatgatcg tggctgcttc caaaggtgca
    15121 acagaggtga aggacaactg gttgaatttg gtatgatcaa tcaatacaaa tgtctcttcg
    15181 gaatttttca tggctaaacg tttaacagct gcctcttctg gatccggagt cgtgaatcct
    15241 tgttcaagat gggcaccgtt cattcccata aatgctttat taaaatgaaa attttttagt
    15301 tgttctatcg cattagatcc taaaacagca ttagttgcca gctttatcgt tccacctaaa
    15361 ataatggtag caagctccaa atcaactaaa tttgccgcat gttgaacgga atttgtcact
    15421 actgtgatct gttttccttg cagaaaagga atcatttcta acgtagtaga gccagcgtct
    15481 aaataaatga catcttcgtc attaatggtt tcagctgccg atttggcgat ttgctgtttt
    15541 tcgtgaacgt ttttgagtga tttttcactc atattctgct caaaattcaa taattgctct
    15601 ttcttagctc cgccatgaac tctttctaga aacccactat tctccagctc ttgtaaatca
    15661 cgtcttatcg ttgattcaga agcattcaaa aggtttacaa gatcttgtga tttcacgatt
    15721 ttctgctgtt caagcaagtc taggattttt tgtctgcgtt cctctgtaag cattttcacg
    15781 atcacctcaa tacaaataat agcatatttt ttttctacta gcaatataaa tcagtcaaaa
    15841 acgttcataa aaattataaa tcagtcaaaa aaaaataaaa aaccagtcag aaagactggt
    15901 tttttatttt tttgtcta
//
LOCUS       DAYYCY010000057        15303 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000057 DAYYCY010000000
VERSION     DAYYCY010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.057"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(261..1043)
                     /locus_tag="ACHAZB_001403"
     CDS             complement(261..1043)
                     /locus_tag="ACHAZB_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8614088.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(1108..1944)
                     /locus_tag="ACHAZB_001404"
     CDS             complement(1108..1944)
                     /locus_tag="ACHAZB_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM8614089.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(1962..3371)
                     /locus_tag="ACHAZB_001405"
     CDS             complement(1962..3371)
                     /locus_tag="ACHAZB_001405"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8614090.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3385..5208)
                     /locus_tag="ACHAZB_001406"
     CDS             complement(3385..5208)
                     /locus_tag="ACHAZB_001406"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFM8614091.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(6051..6377)
                     /locus_tag="ACHAZB_001407"
     CDS             complement(6051..6377)
                     /locus_tag="ACHAZB_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8614092.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(6423..6785)
                     /locus_tag="ACHAZB_001408"
     CDS             complement(6423..6785)
                     /locus_tag="ACHAZB_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFM8614093.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(6822..7037)
                     /locus_tag="ACHAZB_001409"
     CDS             complement(6822..7037)
                     /locus_tag="ACHAZB_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8614094.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            7164..7499
                     /locus_tag="ACHAZB_001410"
     CDS             7164..7499
                     /locus_tag="ACHAZB_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8614095.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(7575..9572)
                     /locus_tag="ACHAZB_001411"
     CDS             complement(7575..9572)
                     /locus_tag="ACHAZB_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8614096.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSVEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(9670..9945)
                     /locus_tag="ACHAZB_001412"
     CDS             complement(9670..9945)
                     /locus_tag="ACHAZB_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8614097.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            10177..10911
                     /locus_tag="ACHAZB_001413"
     CDS             10177..10911
                     /locus_tag="ACHAZB_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8614098.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            10950..12092
                     /locus_tag="ACHAZB_001414"
     CDS             10950..12092
                     /locus_tag="ACHAZB_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8614099.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(12187..13578)
                     /gene="sufB"
                     /locus_tag="ACHAZB_001415"
     CDS             complement(12187..13578)
                     /gene="sufB"
                     /locus_tag="ACHAZB_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFM8614100.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(13583..14053)
                     /gene="sufU"
                     /locus_tag="ACHAZB_001416"
     CDS             complement(13583..14053)
                     /gene="sufU"
                     /locus_tag="ACHAZB_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HFM8614101.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(14040..15275)
                     /locus_tag="ACHAZB_001417"
     CDS             complement(14040..15275)
                     /locus_tag="ACHAZB_001417"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFM8614102.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
BASE COUNT         4681 a         3127 c         2528 g         4967 t
ORIGIN      
        1 cagtactgtc tagtagtata aaatatacaa atttcttaaa aattttttca ctaaaatcac
       61 tacctgaaac agtctaaaac acttatatat caacgtttaa tgaaactatc tccgtttcat
      121 ttcagataca aataaatata gtcgggctcc aaatccgaaa aaaaataccc ccagtagctt
      181 atcctaagct actggagatg tataagtacg gtcatataac aacggggtag ggtaaataaa
      241 tgaaaaagat ggaagaatca ttattcaata acttttttaa atacttcgtt gtaatcagtt
      301 acctgtccgt tcttaaatac cacagaataa tcttttttcc cattttcaag taaccgaagt
      361 ggaatgtctt taccaacttg ctctgtcact gcattactga tacctgttag attagctaat
      421 aaatctgata aagattgagc atctgtagaa atctctgtat cagtatttat cgtgattcct
      481 ttatcttcat ttacaagctt ccaagtaggt ggtatagctg tgtatgtttc gatgaatgaa
      541 tcaattcttt catctatcgt cttcccagta tctacttctg tctcattact cttagatgaa
      601 tcgattcttt catccatcgt ctgctccgta actgcttctg tctcattatt ctcaccggaa
      661 actctcttca cacccaaact tatatattgt ccatctttag tgtgcatagt taaaattccc
      721 acgcttttac taataaattc actataacca gttctttcaa tagagacttc aatgtatttc
      781 acactatcat actcaaaaga aaaatagaca tgtaaaagca ttttgttgtt tacatctaca
      841 taataatcct ttgactctac ttgagacctt cctatacgga atacagcttt actgtctgat
      901 atctctatac cagcatcctt attattaatc tgccaatctc caagtagctc ttctggcaag
      961 ctagtagttt cttgtgaaca ctttaccact tcactggtag aggtcgaaac attcacctcc
     1021 gcactgttag aagaattttc caaggaatca ggagccttag cacaaccaac aagtctttat
     1081 atattgtgca gccgatattt tcttcattca gtacaccctt gaaatatgca gtgttagata
     1141 aagcagttcg tcctgatttg ctgaggcacc catctcttct tgaacgtact ccttgatttt
     1201 caaggccgcc cgatactctt tcggaaaacg ctgcttcact gaagccagca aaaaatcatc
     1261 cttctccggc aactcttcct tcttaatcat tcgggcaata aaaaactgta aatgcgtcac
     1321 aaatcttaag taaagcatcg aatctttatc aacctttttt tcgggaaaca gatatcctag
     1381 catagccaag atatccttta gtgctcttac cgagagcatg gtttcttcca tatttgcttg
     1441 ttcactttgg gcatttacaa tgtgaaacgc aatgtttcca gcctcctctt ctggcaactg
     1501 aacattcatt tttttgttga tcaattggat agcatagcag ccgatctcaa attcttttgg
     1561 gtaatacttt tgaatttcaa tcagcaggcg attttgaaaa acaatgcctt tttgactgcg
     1621 ttcaatggca tgcttcaagt gatcaaccaa ggtaaaaaat agacttttat tcagcttaaa
     1681 gtcaaacttg gcgttggcat aatcgataat tcgcttgacg caaataatta gatcatccgg
     1741 agcagcctca aacagtttaa tgtaatcctt ttttagagca tcagaaagtg aatctaattt
     1801 aaatactttt tggattttgt cagccggaac caaatcgcct ttctttagtt gaaatccaat
     1861 ccctttcccc atcaaaatta cttctttgcc ttgcttatct aagcaaagaa ttaaactgtt
     1921 gtttaacgtt ttaattactc tcattagatt caccgttctc tctaagctaa gccatttctt
     1981 gcgattactt tttgatacca aaagaagctt tcttttttta ttcgttttaa ttctttaaga
     2041 ttctcatcgg ttcggtcaac atatataaaa ccataacgct tattgaagcc ttgatgggta
     2101 ctaacaacat caatcgctga ccaaggtaaa taaccaaaaa catctacacc atctgttaaa
     2161 gctgcttgaa tagccagaat atgtttggct agataatcaa ttcgatcctg gtcattaatt
     2221 cgtctatctt cctcaactat gtcgggtgcc ccatagccat tttcagtaat taaaataggt
     2281 aaatcatagc gatcgtacaa tttcctcaat gttaatcgta gtccagtagg gtcaactacc
     2341 cagccatatg gcgttttatc tacataagga ttttcagctg cccggtagac accttcttct
     2401 cccaacatga tctgttgatc ccccgctcta gcggaaacat ctgatccgtc atttttgctg
     2461 gcagcgatgg tggcagttga ataataatta atcgcaataa aatcgggttt accggaggat
     2521 aaaattgcct gatcctctgc ctcaatttta ggatataatc cacgatcatt caaatagttt
     2581 tcaaacaaat aattgtattt tccccgaact gctacatcta agaaagacca gcctctcaat
     2641 gtttcccaat tatgggctgc aattgcatct tctggactgg aggtagcttg atacatagcc
     2701 gttaaattga ttgctggacc gatttttcct ttaagattca actgatgata caagtgaatt
     2761 actctagctt gagctaataa aacatggtga ttaatttgaa agagttcttt ttttgaaggc
     2821 agctctccac ttggtggcaa accaatcgct ccaggatgaa ggatcatcgt gttttgctca
     2881 ttaattgtca gccaatagtt caccttgtca ccatactcag taagcaatac ctgtacatag
     2941 tccacaaaat aatcgatagt tgctcgattt aaccaaccgc cctgctcctc tagaaaataa
     3001 ggtaaatcga aatggtacat ggtgacaatc ggttcgattc cctgattcag gcattcatca
     3061 attacttgat gataaaaaga aattcccttt tgattggttt tattattttc aatatccata
     3121 attcgcgtcc aagcgatgga aaagcgaaaa gcttttaagc ctaattcttt aaacagctgt
     3181 atatcttctt tatagtgatg gtaaaaatcc gaggcaactt caaaattagc taccccttct
     3241 gtatgctgat acttgtcaat aatagacggt tgtttcccat cctcctgata ggcaccctct
     3301 gcctggtagg cactaatgga ggcaccccat aaaaacttct ctggaaatgc tgaaagctgt
     3361 ttgtattgca caaaaatcct ctccttaact aatttgcatc aatggttcgt gttcctttaa
     3421 gcttccgctt gcttcactaa ttcctgcgta attttcactg ttggtcacta ccacaatcac
     3481 tgtatcatcg tagccagcgt ttctaatggc agcgaaatcg gcgttgatta ataattgtcc
     3541 tgctgacacc tgatctcctg cttttacaaa tacttcaaag ggttcccctt ttaaattcac
     3601 ggtgtcgatc cccacatgga tcaataattc aataccttct ttagaacgta gaccaatcgc
     3661 atgtttagat tctaaaatag taatgacttc tccctcaaca ggtgaataaa tgctgttttc
     3721 ttctggatga atggctacgc cctttcccaa tagtccctta gaaaagacag catcgttcac
     3781 ttcagaaata ggacacacgg ttccttttac cggattttca acaatgactg cttcggctat
     3841 tgccgtttgc tctgattcat cagaagtatc gtcaaatcca attagataag caatgataaa
     3901 ggttgcaaca aaggcgatta cacacgtaat aattgcgtga acaatattca ttggattttc
     3961 tccaataaag gctggtaagg ctgctaagcc aggggaaaca aaggcatatc tgactaaacc
     4021 gctcaagcca gcatacaaac cagcaattgc accaccaatc atggaagcaa ctaatggttt
     4081 tttcagcttc aaggttactc catagagtga aggctcagta attcccatta aagcagtgat
     4141 cccagtggac acagctagct gcttcaactc tttgttttta gttttcgcgg ctacagcaaa
     4201 ggtagcacct gattgagcaa tattagaagc cagcattcct ggcccattga tcatttcaga
     4261 acccgtactg ctcaattgca tagtagcgat tggcgtcatt gcccaagctg tacccgtaac
     4321 caccaaaaat ggctgcaaac cacccatcaa aaatggaatt aaccaagctg ctttgccgtt
     4381 aatccattca gcccccattg ctataagatc gttcagcaac gttccaaacg gtccaatgac
     4441 aaccaacgct aaaggcccca taattaaaaa taccagcatc ggcttagtaa agaatttaat
     4501 aaacgaaggc gaaattcgat ccgcaaaggg ctctacatag gacatgatcc aaacagtaaa
     4561 gataatcggt aaaactgatc ccgcataatc tgcagctaat accggaattc ctagaaaaga
     4621 aacggcatcg ccagtagaaa aaagctgtga aatatttgga tgaagcaaaa tgccagcaac
     4681 agtaatcgcc aaaattgggt tgacatcaaa ctttatggct gccccatagg ctaatagtac
     4741 tggcataaaa taaaaggctg catcagcaat agtattcaaa atgatgtaag tttgactact
     4801 gtctgatagt acaccagtca aagtcaagac ggccaaaatg gcttttacca tcccggcacc
     4861 tgttatagct ggaatcatcg gtgtaaaggt ggtagaaatg acactaatca atcttgaaat
     4921 aatactttct tgattgtctg aagaggggcc ctttttaatt gaagttccca cttccttttg
     4981 aatttccttg aaggctgttt ggacttcatt gccaatcacc acttggtatt gtccaccgct
     5041 atccaccaca ctaattacac caagtgtttt ttctaaggct ttcgtatcgg ctttcctttt
     5101 atctttgagt tcaaaacgca gtctggtggc acaatgggtt aagctgacca cattttcttt
     5161 tcccccaacc tgttgcaata catttcttcc tacttctctg taatccatta ttctctctcc
     5221 ttaaagcagt aaaaaagacc aagtcaaacc acgccccaat caacattgaa gtgttagttt
     5281 gtcttggtct tgcctgcata atcagtaaca tgccaataac gttattttgc tgtaatccaa
     5341 taatagcaca ttatgatagc gcaatcaaca gggaatttaa ttttttatct tttcgtttga
     5401 ccgtataggg taatataaca gttagatcac atcaactagg gtatatctgc gtatacttac
     5461 tgctaaaata agtatatcta atattaacta taccagtaaa accattccta cataaccagc
     5521 gggaaattcc caaaatcgtg aaagcatcaa aaatgcatat aacggatcac acaacttcaa
     5581 caattctgct cttgggaaaa attgatgaat ttggtgagta aaggatggaa taaacagcat
     5641 tccaacaata taggcaacaa tagtgatact tgtttttttg gtaaaaatca gtaagcaata
     5701 gccaatttca atcattccga gatataaagg tatcgataga atgatttggg ctagtattct
     5761 taaaaattaa ctagcaaatg tttaaactga tttatttcta atctacatgt agtaagtatt
     5821 tcaattccac caattatcaa taaaaatacc aaacatatcc ataataattt tttctttttc
     5881 atttctttta tattgttata acagtgaata acataaaaat tcccgacaat cattgtaata
     5941 tttttttgtc acctgtatta gtatttaatt tataggaaac tataaaaact ccctagtgaa
     6001 tttacacctt tgggagttcc tgtttggacg gtatgcataa tacatttaaa tcaatttaac
     6061 attaacacct cagcatatgg tatctccaca tatatacggt ctttctttgg atagtactca
     6121 atatttatgt tgatagcact tccattgtct aatatagctt tagcccggta tgtgttatct
     6181 ccattttcat gagtaatcgt cacatcatca catgatcgat ttaagttgta ctgttcttgt
     6241 aaaatctgtg ttaccaatgg ttttgatgac ttctctaatc cagatgtacc tgtcattttt
     6301 gaaaaaataa tacctataat agccaagaca acaataccta taatgacaaa cttcttgttc
     6361 atatttactt tattcaactt tttatctcct atctccaaaa taatttgctc ttatccctct
     6421 tatcagtaca ttgacatccc taaaagtaca ccaaagattg ctccagctac ccctgctatc
     6481 atcaaaatcg tacccatttt ctcatctttt tctcgatata agtagcccat aataacagca
     6541 acagcaccaa atacaacagg aataaatgct aacgatacta ccatagaaat ccaaccaata
     6601 atcaaataca gcttccctgt ctcttgtcca tgtacgattt gttccttcat ttcattaatt
     6661 gtctgctcag tacttttctt tactgtgtct gttccggttg tctcatctgt ttcttgtagt
     6721 gctccacaaa actggcaata cttactttca tctgttattt ctttgccaca ttttctacaa
     6781 tacatgtctt ctcctcctaa aatcattcaa ttgcttatta gttaactgtt tactttatta
     6841 aatacatcag aaaacagctc attcattggt acacttaata tctcagaaag catcttcatt
     6901 tgatagggtt taggaatcgt tctccctgtt tgccacgaac ttacacattg ctgtgtaaca
     6961 ttcatttttt ttgctaactc cgtttgagaa agtccttttt cttttctgta tcggctgaga
     7021 ttacttactt ttttcatgat tctcccctcc tgttttatta tatacaacaa tatgatgtat
     7081 ttcaactcat aaaatataaa aaacttcaaa taccattaca aatacaacaa cttgttgtac
     7141 tatactgaga tgggggtgta ttaatgaaac agaaacaata tgcaaaggtc attagagaaa
     7201 agcgaaaagc tctcgggtgg acacaaaaag aattagctga aaaaatcttc tctacacaac
     7261 aggcagtagc aagatgggaa aattctgtaa cagagcctaa cctcgatagc ttaacagctc
     7321 tttctagagc cctaggaacc cctgtaagcc attttctaga caatgttgtc gtagattatg
     7381 aggaagagtt tttggctctc tatcgttctc ttagcactga agatgcagtt cgaacaattg
     7441 actatatgaa gctattaaaa aggcaagaaa atgaacgcaa tcagctattg aaagattgaa
     7501 aaataaataa cccaccgaac tttaacggtg ggtgtattga gatgaaataa gattaagagc
     7561 tatggataac ctgtctagtt taatacaatt gggtcttttc tcttccttac tctccgtcca
     7621 tcatctcttg ttacacttgt aaattgatac agaggaacta tcccagctcc tgataatatc
     7681 aattctttta ttgtttcatc tatccctgcc cctttcgcta aatatattaa aaaactacta
     7741 cttgctttca cattgtatag tttcttaata tccttagaag ctcgttcaag agtgaatcct
     7801 tgtttaacaa agtagcctct gttttttatt aaagcatttt tcacgctgtt atccccaaca
     7861 ttacgtaggc gttcacaaac agtgttaata agttctttct ctgtcattag atatgtccct
     7921 tgccacgata agccctgtaa aacttcatgt gcttcatcta aaatcaattg tttttccttg
     7981 ctgagttctg atcgggaatt ttgaggaaat actcttttag cctctttttc gttaaaacat
     8041 atcttaaccc attcataaga aaaatattga tatgtaaaat gaattttttc cagaagtgga
     8101 aggatatctt cattcaattt ttgaatcaca gaagttgcat taaaaacgtc taatcgatag
     8161 acattgcttc tcttatgatt aaagtttcct aacgctttct cttcctttaa cctatttagg
     8221 tattcgagca tcttcaaagg aacatcttca tcatctaatt tttttatcaa tcctataaaa
     8281 gtcataagtg atagtaactt attacattta tctatgtcat aagttttacc atagtaagaa
     8341 ctcatttttt tagctaattg ttcccccgac aaaatacaag tcacactttc cttactatca
     8401 tcaaaaaaat taacatcaga aagcaagaca tcatagacac gtcgcatttc ctctagataa
     8461 ttccttattg tcctgaaaag tttgtctttc attgattcaa gagtgtcaaa aagtactttt
     8521 gattcctctt tcaattttcg aaaatgtgta ggatacaagt ctaggttctc taatagccaa
     8581 ttaaatgttt ctacccgact gtctttcttt aaaattagca tcacaactcc aataaaattt
     8641 tcactgatat cacaattact ggaatgacaa taaaaaatgg aatgcccagt tttactggta
     8701 aaaatactag cagagggatt atgatcgttg tgaaacagac atcttaagtt tttacttgac
     8761 ttaagtaatt tattcatatc aatggttaac aatctttcat aaatctcatt aaaagacatg
     8821 tcggaggaat caaacaggca gtctttaaac cactgtttca tttcttcttt gtcgtcattt
     8881 tttatcaact taacaaaatc ggtttcattg actagttcat actctctccc tgaatcactt
     8941 tgacaatttt tagaacatgt catggtaaaa tcaatcggaa gctcagtaat atctaacatg
     9001 ttgtcttcat taattattat cactgcatct gacttatatc ctccaaaaaa cattctattc
     9061 gaagcattta cattgtcatc acacccgggg atgttctttt tcaagtattc gtaaacagca
     9121 accattttac catagtcagt aattgcttct tttaatagaa agacaatctt gtatttttct
     9181 agattttcag agtaccgaat acttttttgt atcgcaaacg cagaattatg aactaaattg
     9241 atagataaaa ccttttctac gcttaagtat ctactatcat ctttactcaa atctactacc
     9301 ttccccttct catctttaac agtgttatcg acatccagaa taattactct ggtttccaca
     9361 aaactctcct tgtcattgcc tttattcttt gtcttgcttg gaataaatga ttgtgatgaa
     9421 tttagtatct ctacaaactc atctcttgta acttctctat agttatctaa acacctatct
     9481 cttacatcag ttttttcttt ttttgtaact ggcttctcta ataatttttc ttgatctaca
     9541 taaattggaa tattaatcat tgatgtttcc atttgtgttt cctccttata aaattctgtt
     9601 aaagactaaa acatacagat aataaaaaca aactctttat tactatatgt tttagtctta
     9661 tttgtttact caacctttat accgttccat gaaactatca atatcagatt ttttaaatct
     9721 aatgaccgta tcaattttta taacaggtaa cccgtattga gaaacatatt ttctaatcgt
     9781 tcctactgat acacctagat attcacttgc ctgctgatag tccatatact ctagagtgac
     9841 tccagaaata atgtcttcta gcataggctt tagcactgta attatctctt tcacttgttg
     9901 ttcaacttgt atagaaacaa tgctatcaat gatttgtaga gacatatttc cccctccttt
     9961 cgtgattgct tgaacaaatt ttctaagaat tatcttcgta ttttttgctc aaaataaaag
    10021 tattttgtta ttaaccgatt aatcaaacaa aaaatgatta ccagttcatc gacatacaca
    10081 taatacctcg ataaaaaagg ggtgtcaaca gctttgtaaa atttatttgc aacacccctt
    10141 ttttgaggta tactaatttt aggaggtgtg gtcaatatga taaaacgtcc accaataaat
    10201 tatttagaac gaaaaaagat actcggaaca aaaataaaag ctataagaaa gagtaaaaaa
    10261 ttaacacaac cagcatttgg tcttatgatt aacaacggtc aattaattga caaaaaaact
    10321 atctacgaat gggagaaggg aacctattta ccaataccag agcgattatc ccgaattgct
    10381 gatttgggaa atatgagtat agaggagtta gtttgtggca atgtagaaga atatattttg
    10441 ggaattattc tttatagaga tagcattgta ctagatggta taacatttcc agataaaaac
    10501 ctgttccagc atcttcgtca acagttccca ccagtacact ctaatttaga tacatggctg
    10561 gatagatatt ccaaattaga accagagatg caagaattta ttgctaacaa gacttgtaat
    10621 aaagttaaaa atgaaaaaat tagtcttttc aatatcttaa aaatagaaga attattcatc
    10681 aatgctattg tagaagaatt tgacaataac attcttttcc tcactagcag tatagaagaa
    10741 ctactagaaa gaatggtaga tgaatggcta cctattcaat tgaaagacat gtcttatccc
    10801 gaagaagctg tgagagaaat tactgataat ataaacaagc tagaacaaac gatttcatca
    10861 attgggaaaa aatacactaa gaaaaaaatg aagggaggtg ataccatcta gtggtaagta
    10921 ctccttgtaa actaagagga gatgatttaa tggcaaaaat atcaaagtac aaaaagaaaa
    10981 acggtcaagt ggcgtggatg tataaaggac acattgccac cgatccacga actggtgaaa
    11041 aaataaatac tacgagaaga ggttttagta gcaaacaaga agctcaaaaa gattatgacg
    11101 agtacagaca tcaaatatta tacggtgtta aaaagaaagc acctgatatg acatttgaag
    11161 acctgtataa tgaatggata actcatcagc gtaccagtgt taaagcttca acaatcgcaa
    11221 tctcagtaag atacgcaaat aatcaaatac ttccagcatt tggtaagctt aaactatcga
    11281 atatctctgt cccatattgt caaaaagtgg tagatgaatg gcacagtaaa tatgaaagtt
    11341 acgattatat gagaaaacag actgcacaaa ttttacgcta cggggttgca atgcaatata
    11401 ttgacaataa tcctatggaa aaaacacttc ttccacgaaa aaaagagtat gagaaaaatc
    11461 gtaaatttta ctcaaaagag gagcttaata atttactgga tgcttttaaa gattttggta
    11521 acatgaaaca atacgccttc ttcagattat tggcatacac aggtatgaga aagagtgaag
    11581 tactcgcttt acagtggaaa gacatagaca cgtttaataa agaactacat gtcaataaaa
    11641 ctcttgctgt agatgaattt ggtaaagtta taattcaatc tccgaaaaca agagcttcta
    11701 gacgagttat ctctttagat actgagactc tttctatcct caacaactgg aaattacagc
    11761 aaaaagaaga gtatttaaaa cttggataca atactagctc taaagagcaa catgttttta
    11821 caacagttaa aaacactcta tacattccaa acactgttaa tgattggtta agatatattt
    11881 tgaaaaaata taacttgcct cgtattacac ctcatgggtt tagacataca catgctagtt
    11941 tattattaga agcaggagaa tctgtcaagg tagttcaaca acgattaggg catgaaaata
    12001 gtaaagtgac attagacatc tatgcacata ttacaaataa tgcacctaag aaaacaggtc
    12061 aagactttgc tgatatgatg gctcatcagt aaaagagctg gtcaaaatct ggtcatttct
    12121 agtatacagg caaaacaaaa aagctagaac ccttatatat caagggttct agctcctata
    12181 acaggtttac ccaacactgc cttccatctc ataactgatc aatcgattga gttcgactgc
    12241 atattccatt ggaagttctt tggtgaatgg ttcaacaaag cccatgacga tcatttcagt
    12301 tgcttcagat tcggtcaggc ctcggctcat cagatagtag agttgttctt ctgagatttt
    12361 tgagactttg gcttcgtgtt ctaaagagac ttggctgttg tggatctcat tgaatgggat
    12421 cgtatcggat ttcgaccatt cgtccatgat gatcgtgtca cattcgatat gagagatcga
    12481 acccgcactg tttttggcaa aggttacttg tcctcgatag tttacttctc cgccgtcttt
    12541 tgaaattgat ttagagacga tagagctaga tgtatttggt gcattgtgga tcatttttgc
    12601 acccgtatct tggatttggt ttgctcctgc aaaggcgatg gagagcatgg tgcctcgtgc
    12661 accttttcca tctaagtaaa cacttgggta tttcatcgtt gtttttgcac ctaggtttcc
    12721 gtcgatccat tcaacggttg cgccttcgta tgcttttgct cgtttggtta ctaagttgta
    12781 cacattgtct gaccagtttt ggatcgttgt gtaacggcaa tacgcatctt ttcttgtaaa
    12841 gatttctacg atggcggcat gcaggctgtt acttgaataa gtaggtgccg tacaaccttc
    12901 gacatagtgg atgcttgctc cttcgtcaac aatgatcagc gtacgttcga attgccccgt
    12961 gttttccgca ttgatccgga agtacgtttg taatggaaca tcgactttga cgccttttgg
    13021 cacgtaaata aaggtcccac ctgaccatac ggctgaattc agcgctgcta atttgttgtc
    13081 ggttggcggt acaagttttg aaaagtactc tttgaataat tcagggtatt ctttcaaagc
    13141 agagtcagtg tcagtaaaga cgattcctaa cttttcgaat tcatctttca tattatggta
    13201 cacgacttct gattcatatt gtgcggaagc accggctaga taagctcgtt cagcttctgg
    13261 tataccaatc ttttcaaatg tatctttgat tttttctggt acgtcttccc agtcacgagc
    13321 gggtttgtca cttggttttt ggtaatattt gatggcatcg aaatcgatgt ctgaaaggtc
    13381 ggggccccat gtttgcattg gcattttgtt aaatgtttct agagatttca gacggaattc
    13441 aagcatccat tctggttcgc ctttgattct tgacatctca cggatcactt cttctgtcaa
    13501 gccttctcct gtactgaaaa ccggttcgac atcatcgtga aacccgaatt tatattcttc
    13561 taattctggt acgcccatgt tctcactcct cttccttgtg gtaatgatgg aaagctgcgg
    13621 taccatcgtt ttccattgct tttcctagtg ctttccaggc aagtgtcgca catttgatcc
    13681 gagcgggaaa tttagctaca ccaccaagca tagcagcatc gcctaattgg tcgggatctt
    13741 tgacttcttt tccttggaca agctgtagaa agtctacagt taaatgttct gcttcttcta
    13801 ttgattttcc gatgacggcg tctgtcatca tcgaggcact tgccgtactg atggagcagc
    13861 cactgccgtc aaaggcgata tttttgatga ttccgtcttt tacatcgact tgcaattcga
    13921 tcacatctcc acatgtgggg ttgttcaatt cgatcttgct tgtcgagtca gtcaagcttc
    13981 cacgatgatg cggatgggaa gaatgatcca agatcacttg acggtataaa ttgtctaatt
    14041 tagatagtcc catgttggaa aaactccttt gttgccatga ttgcttcaac taaacggtcg
    14101 gcgtcttgcg ttgtgttgta gaaataaaag ctcgcgcgag cagtcgctga tacttcaagg
    14161 tacttcaata gcggttgcgc acaatgatga cctgctctga cggctacccc ttccatgtct
    14221 aaagctgtcg cgacatcatg gggatgtaac ccatccaaat tgaatgcaac gactcctgtc
    14281 cgaaaattcg ggtcttgtgg accataaaca gtcaatccat caattgcttg taattttggc
    14341 aacacataag cggctagttc ttcttcatat cgatggatat tgtccatacc gagtttttgc
    14401 aaataatcga ttgcatggcc taatgcgata gcgccggcga tatttggtgt ccctgcttcg
    14461 aatttccaag gtagttcttt ccatgtactg tcgtataatt cgacaaaatc gatcatttcg
    14521 ccaccaaatt caacaggttc catttcttct aaccattttc gtttaccata taaaacacca
    14581 atacctgttg gtccgcacat tttgtgtccg ctgaaagcaa agaaatcaca gtctagtgct
    14641 tggacatcaa tggccatgtg cggggcagat tgagcaccat ctactacgat gaccccgccg
    14701 ttttcatgga caagttcagc taattcttga acaggattga agacacctaa gacattggat
    14761 acatgagcaa tggagacgat tttggttttg tccgtaatct ttttcgctgc atcctccata
    14821 tccaggaagc cttcttctgt tagttcgata tatttcaaga cagcacctgt tcgttttgcc
    14881 aactgctgcc acgggataac attagaatga tgttccatgt aagagagcac tatctcgtct
    14941 cctgctttga tatagcggtc gccgaaggtt ctggctaccc aattcaagct tgtcgtcgta
    15001 ccacgagtaa acagcacttc tgctgtttct tttgcatgga tgaacgcccg tactttttct
    15061 cttgcttgtt cataatcatg ggtcgcgcgt tctgctaacg tatggacacc gcgatgaaca
    15121 ttggcgttat cgtgttcgta atagtacgct aatgcctgga gtacttgttt tggcttttgg
    15181 gtcgtcgctg cattatccag ataaactaaa ggttcatcat tgacgtcttg aaataagata
    15241 ggaaaatcat tgcgtagctt tttagcatcg atcatccatt caacttccct tcgattactt
    15301 cga
//
LOCUS       DAYYCY010000058        15292 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000058 DAYYCY010000000
VERSION     DAYYCY010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15292
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.058"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            78..428
                     /locus_tag="ACHAZB_001418"
     CDS             78..428
                     /locus_tag="ACHAZB_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HFM8614103.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            527..1057
                     /locus_tag="ACHAZB_001419"
     CDS             527..1057
                     /locus_tag="ACHAZB_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFM8614104.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            1211..2362
                     /locus_tag="ACHAZB_001420"
     CDS             1211..2362
                     /locus_tag="ACHAZB_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFM8614105.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(2471..4510)
                     /locus_tag="ACHAZB_001421"
     CDS             complement(2471..4510)
                     /locus_tag="ACHAZB_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFM8614106.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKIAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLGNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(4568..5125)
                     /locus_tag="ACHAZB_001422"
                     /pseudo
     CDS             complement(4568..5125)
                     /locus_tag="ACHAZB_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     regulatory      complement(5273..5439)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            complement(5664..5870)
                     /locus_tag="ACHAZB_001423"
     CDS             complement(5664..5870)
                     /locus_tag="ACHAZB_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8614107.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            complement(5886..6500)
                     /locus_tag="ACHAZB_001424"
     CDS             complement(5886..6500)
                     /locus_tag="ACHAZB_001424"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFM8614108.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(6647..7330)
                     /locus_tag="ACHAZB_001425"
     CDS             complement(6647..7330)
                     /locus_tag="ACHAZB_001425"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFM8614109.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(7439..7897)
                     /locus_tag="ACHAZB_001426"
     CDS             complement(7439..7897)
                     /locus_tag="ACHAZB_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFM8614110.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(7981..9744)
                     /locus_tag="ACHAZB_001427"
     CDS             complement(7981..9744)
                     /locus_tag="ACHAZB_001427"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFM8614111.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(9965..11425)
                     /locus_tag="ACHAZB_001428"
     CDS             complement(9965..11425)
                     /locus_tag="ACHAZB_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8614112.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(11633..12559)
                     /gene="glsA"
                     /locus_tag="ACHAZB_001429"
     CDS             complement(11633..12559)
                     /gene="glsA"
                     /locus_tag="ACHAZB_001429"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFM8614113.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(12582..14009)
                     /locus_tag="ACHAZB_001430"
     CDS             complement(12582..14009)
                     /locus_tag="ACHAZB_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8614114.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(14040..15254)
                     /locus_tag="ACHAZB_001431"
     CDS             complement(14040..15254)
                     /locus_tag="ACHAZB_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HFM8614115.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         4371 a         3073 c         2637 g         5211 t
ORIGIN      
        1 gaataatcgc tttttcttct ctttttctta tatactataa gtagtaaata atttgaaaaa
       61 aagaaaggtg actgattatg gcgcaaaatc gtatttttac gacaagtttt gcaagcgttt
      121 atcctatgta tgtagcaaaa gcggagagaa aagatcgtat aaaagaggaa gtagatgaaa
      181 tcatttgttg gctgacgggt tattcccaaa cagaactaga atatcaacta caaaaacaag
      241 tgactttcga acaatttttt gcggaagccc ctgtccccaa tccttcacgt tccttgatta
      301 caggtgttgt ttgtggtgta cgagtagaag acatcgagga taacttgatg aaagaaattc
      361 gttatttaga taaaatgatc gatgagctag caaaaggcaa aaaaatggaa aagatcctca
      421 gaagttgatt tttccagaga tattcttctc cttcgctcat tttgtaacat ttaatttggt
      481 aaaatgacta gttcacgtta aaataagtca aaggaggttt tcttatatgt ttgagacgat
      541 taaacaaaat attcaaaaat acgccttatt aagagctgct gtttatatta tttctgggat
      601 tgcgattgtg ctaaaccctt ctgctgtttt tcattttatc ggttatctga tcaccgccta
      661 tcttgttttg cttgggttgt tgaatctttt tgaggcttac cgttttaaaa ggaaaacagg
      721 ttcttggagt atcggtctgt tcagtggtct ttttttcctg atcttagctc tgatcgtcta
      781 tgcttttgca cctgcaatcg tttctatcct gccgattctt cttggattag ctgttatcat
      841 caacagcttg ttccagttgt tcttcagcat gaatacaaaa gctaaaagtt ggagtgttta
      901 tagtagtatt ctgttgttga ttggcggatt cgtcttattg ttcaatccat tcaaaagttt
      961 gatgcttcta ttccaaatat tcggctttat tttgattttc atgggcatcc aggagattat
     1021 caatttcatc agaataagaa aaatgactat acgttaatca tttttagtta ttaaaataga
     1081 ccgtataaaa accaacaatc caaaaaaatc ttttggattg ttggtttttt tgtgagtata
     1141 ttttcctatt ttttcaataa tgtatgttat gctaagttcg ggctatattt tatacaaagg
     1201 aatgatgaga atggaattta ttggtatttt atgcctgata ttgatttcaa caacgattgg
     1261 ctcacatatc tcgcggagat taggtatccc agcagtgatt ggacaattac ttgttggtgt
     1321 tttgctaggc caagctggat tgaattgggt caaacctgat attctagtcc atgacttttc
     1381 ggagattggt gtgatcttat taatgttttt agctggtctg gaaagtgatt tatctctttt
     1441 gaaaaaatat ttccgacctg gtatgttcgt tgctttgctt gggatcatct tcccggttgt
     1501 ttttggctgg ttgacaggca aagcattcca agtttcggga aatgaagcga ttttctttgg
     1561 gattattctt gcggcaactt ctgtcagtat ttcagtagaa gtattaaaag aactaaacgt
     1621 agtgaataca aaagaaggat caacaatttt gggtgcttct gtggtagatg acattttagt
     1681 cgttttagtt ctaagtttca gcctatcttt tctaacgggg gaagcttcta gcaatgcttc
     1741 tttaccgctt gttttgattg aacagctaat ttattttgtt tttattttct tattagtcaa
     1801 atggattgcg cctttcctac tttcatttgc agaaaaagtg tacgcaaatt cttctattat
     1861 catcgtatcg ctagttattt gtttaggaat gtcttacgta gcggatcttg ttgggcttag
     1921 ctctgtcatt ggtgcttttt ttgctgggat agctgtcagt cagacaaagg ttaaagaaga
     1981 agtataccac aatgtagagg ctttaggata tgcagtattt ataccagtat tttttgtcag
     2041 tgtaggatta gaagttgatt tttctgactt tagtgaacaa ctactcttta tttctatatt
     2101 gacgcttgta gcgattttga ccaaattgct tggaggttat tttggtgctc agattgccgg
     2161 tttttctcaa catagttcat tgatggtcgg tgctggtatg atttcgcgtg gagaaatggc
     2221 tttgatcatt ttacaaattg gacagcaaag tcaattgatc gaaccacagt actattcacc
     2281 tttagttatc gtgattttat taagtacact tatttctcca cttgttctca aatattttac
     2341 aaaaaaagtt tatgaagtgt gagtaaatca aaaagaagtt gtgaaagaga catcgtgaaa
     2401 accgtttatt tgaaaaaagg actgtgacga taacgccaca gtccttttac tgtacttaaa
     2461 aagcaatttt ttattgataa ttttggttga ggtattgcaa tagttccgat gctcgtttgg
     2521 ttgctgaatc atcatcttct ttattttcca acatgctgac catcatataa ccttggttat
     2581 caagattgaa agcaaacaag aaactgttct cttgcccctt tacatcctgt ttttctttga
     2641 tttccgctgt accagttttc gctgccaacg gaatccctaa agcagaaaga gaatgtgctg
     2701 taccatttac atcttgcaca acttctctca gatctggcac gatcgtttgt actgctgttt
     2761 cgctgatgac attcttctta tcttttgtct ctttatctgc aatcaattta ggatagacaa
     2821 gtgtgccatt gttggtaaaa acagaataca ttgctgcttg ctggatagga gtaattagaa
     2881 gttcgccctg tccatatcca gtatcagcta gcaagatatc tgagttaaag ctatcttcat
     2941 tagaaatttg tgctggattc atactaattg gcaaatcaag gtcttcacca aaaatgaatt
     3001 tatctaagcc tgtacgaaat tttttctcac ccattttcaa cgtttcttgt gccgcatata
     3061 tattatcgga atagatcaaa gcagttttta agtctacttg tgatacatca ctactgacac
     3121 gcgttgtttt aaaagatccc caagaactgt ctttttgcca tttaagcccg ttgatcgtca
     3181 acacttcatt tggatcgata gtgccgttgc cgagaccgat tgctgctgta atcattttaa
     3241 acgtagatcc aggagcataa cctgtcgcaa atcggctgat gaatggatgt tcaggatttt
     3301 cttcataagc tttgtaatct tcttgtgaga tcccgtttgt cattttgttt ggatcatagc
     3361 ttggagagct agcaagcgca agaagatcac cggtttttgg cgttgtcgca acagttgatg
     3421 cagcttttcc tcctagactg tcaaaagcta ttttttgtgc cttcgcatcg attgtcaatt
     3481 tgatatcttt gccattttga acttcatgtt cgatcagaac ttttttctct acaccgtctg
     3541 catctgtaat gcttaatttc ccacctgttg tcccacgaag atccttatca aaagccatct
     3601 ccaaacctga gcgaccgatt tttccattac tgcttaattc tggattctta tcaatatctt
     3661 ctgctgtgat atctcccacg taaccaatca gctgagcagc tgcttcaccc aaaggataat
     3721 atctgccgtc tacttcttga atggtagctc cagctggaag ttctggcgtt gctccatcaa
     3781 tgattttcaa tgggacaaag taatcgggtt gtacccagct ttgtgaaatc gcctgattga
     3841 tagcatcttc tgttaagtcg aaggaggaag caatcgcttt gatattggct gttttttcgc
     3901 ctccatctcc aagtttactt ggaacgactc ctaattgttt tagtttgcct gttgttgcta
     3961 atggttcacc atttcgatct ataatattcc ctcttgctgc ttcttgtgtg gtcaggctta
     4021 ctttgtcatt tccttccatt tctggaaaaa ccaagttggg ttgccagttg atcgtggttt
     4081 gaccatcatt tctgtctaat gttcctttat aagaaagatc tttcaattca cctaatgagg
     4141 tattcatctt tgctttatat gaaaaactgt aagtagaatc gtctttttta tctactttta
     4201 gattcgatat ctgaagtcct ttgacatcag cagcaccgta tatattctgg tatttatcta
     4261 agatctcttt ttcagataat gcacttttat ttgccgcctt ttttgatgtc atttctgcag
     4321 ctttgttata atctcccttg ttcaaagctt ggacaaattg ctcaactgtc ttttctccag
     4381 cttctactgc ttgggtttct tgatagtgct ggtagtaaaa ataaccgcca cccgcagctg
     4441 ctattaagat cactgcgccg gcaatataag cgcctgttcg atttttgccg tgcttgtcac
     4501 ttcttttcat tttttcctcc ttctcttgct tgtcattcct ttatttcact atttatacct
     4561 gtttattcta acatatgtct ttgaggaaat ccataccaca atccagaagc acactttctg
     4621 gaaggttcgt ttggatacta ccaagtaatt tcccaaccgc tttgggtagc ttgaatattg
     4681 aagttacgtc tttcagctgc tgagagatag ctttgatcac ttgctgctgc cttctgatcc
     4741 tcccgaagtc tccttctttg tcttcccgaa atcgagcata ctggagtaaa gtattaccat
     4801 ccatcacttg ctctcctttg tttatatata cgccatctaa atctagagat ttttctgcat
     4861 cgatcttgac tccaccagga aacagttcat taacgcaatc gataaaatct tgaaatgtga
     4921 tacttgcata atagggagct tcaaatttga aattttcctt taatgtttga tttaataaat
     4981 caactccccc ataagagtaa gctgcattta tcttattcat tccgtaacct ggaatatcga
     5041 catatgaatc tctcatgata gagatcagtt ttgcttgttg tgttttctgg ttgtattgtg
     5101 caaccagaag tgaatccgag cgacctccac caatcgatct tttttcaccg catctcctcc
     5161 tcacacagca atccacttgt caggtgagga gcgagtttgt ctcatcaaat aagtcagtca
     5221 caaaaatata aagaaaaggt cagaaacctt ttcgaacaag aattctgacc tagcatcatc
     5281 tcatcgtccg agttttagca ctatacaacg taaagaaaac attcttagct attggaaaaa
     5341 gccttaatcc aacagtttct gttttaccca ttggcgactt tcgacgtttc tgggcaacag
     5401 cctatgtttg tataggagcc tcacctaaca aggttattac tatattctat ttacgagttc
     5461 atgatagact tttcctaatg attaggcaat ggaatttgaa atgaaatagt gaaatttaac
     5521 aatctttaat attttttaga aaaaaataac aaataagttg aaagaatccc gttgcaaatt
     5581 ctttcagttg caccaggatt ctttgataat tttttagttt tatgtgtttt tttatatgtt
     5641 ttttcacttt tcggaataaa tatttatttg ttaaagtaat taaacaatcc gctaatcgtt
     5701 tgatctggca tttctatttc cttaccatct aaagtaacag tagtaacgac aaaatggtct
     5761 tgcaatggcg gaacttctaa gtactgccct tgcagaaaat cattcactgt ttcatattct
     5821 tttttcattt cttttgaatc tgtcgatggt ttatagctta tttttaacat aactagtgct
     5881 cctttttatt cgggacgtgc aatcgtgaaa atttctccta ttgctgcata atcatttcct
     5941 gccaaacggc ttacagcatt caattttctt ggatcgatcc ttcctgtctt atcatcatag
     6001 actgtttcat ttaaatgata tttctgtact tctagcaaaa ataaatcagc tgttacttta
     6061 tttttattcc gtatctcgac atgttcatat aaggatgttt caaaacgaac ttttgcttct
     6121 gtcaatgctg gtactgaaat gattactgaa tcagttaaag tcaagtcagt caggtacagt
     6181 tcgctttcct cattaggcag attggctgct gttttattta cttcttctac gttgtcctca
     6241 tcaaggatat ggataacagc ttctcttgtc tcaagaatat tctttgctgt gtcttttact
     6301 gcatcttgtt ttctctggat cgccaaggat aagatgggtc gattcgaact gacgatattg
     6361 aagtaactga agggtgcgat attcactata tcttttgctg atttagtcgt tactactgct
     6421 actggtctag ggatgactga accaatcagc aacttgtaat tttcacgttc tgtcaattct
     6481 gaaggactcc gttcaaacat ggtttcgcct tctttctata aaattcgtta aaaagcagat
     6541 cttgaagaca aaatgaaaag taatgctttt ttcatcttct tagaatctgc tttaaactat
     6601 ctatcaattt ttcttttttg tcaagaatct gtgccgtgcg tgttttttag ttatttgctt
     6661 tacttgcggc ttcgacatat cttttagtga tcaattgcgg acgggtgata atagatccaa
     6721 ctacgactga aaaagcacct aattcatatg ctttctctac ttgttcagga gagtcagtat
     6781 gtccttccat aattactggg atatcgactt gttctatgat ttgattcaat aaatcaaaat
     6841 caggtgtctg cgtctttcgt gaatattctg tatagcctgc taaagtcgta gacaaaagat
     6901 caaaaccatc ttcttgcgct tgaagcgctt cctcaaaagt cgaaatatct gccatagcta
     6961 accgaccgtg ttcgtgaatc atttggacaa gttcagcagt tgttttttct gtcggacgtt
     7021 tacgagaagt tgcatccagt gcaatgattt ctgcttgcgt ctccaaaagt tcttccactt
     7081 ctttttctgt cgcagtaata aacacttctg acccatcgta atctcttttg ataatcccta
     7141 ttaccggtaa atcaacgatt cgcttgattt cattgatatc ttctgtcgta tttgctcgaa
     7201 taccaactgc tccgccttct tttgcagcca aagccattct ccccataata aaagaactgt
     7261 gtagcggctc gttttccaaa gcttgacaag aaacgacgac tccgcctttg atttgttcca
     7321 gtgtatccac aatatctcct ccagtaatgt tatataaaaa tacagcttca tataaaaatg
     7381 taaccctttt ctattcagga gacaataatg agctatttct ttcagcagac ctttcttact
     7441 aaaagaaaat tacttcaaat tcttcgtata cgaccagctt ctcttctgta aattctatgt
     7501 tcaacgcttc tttataagca tgtttaaaaa actcagcatg gactttccgc caataagcta
     7561 atgtccgatc cccttctcct tctttccaag catgctcttt cgtgacttca ctaaaaggca
     7621 agatggtcac atttatattt ttgatgatac aaactggttc attttttcca ttcaggacca
     7681 cgtctaattg accaactttt ggtaaaggct cattctcttg tttatacaga tcgtaaccac
     7741 ttgatgtacc agtctttatc ttttttacaa ctagttcgct caactcgtct gccatttttt
     7801 ctgtatcccc aaatgaaaag aaacctaatt tttctgattg ataactagga tatttttctt
     7861 ggaacttttt gacatattct tctacttgct gatacataac atccacccct ttgcttttta
     7921 taatgaaaaa accatccaga tactagtata aacaattatc tggatggtga gcataatgaa
     7981 ttattgaata ttcaagacac gcagactatc ttcaacgatt tgttttggtg ttaaatgata
     8041 acgttcataa agctgcccta caggaacgct atccgtgaat tctttctctg caccaaaatt
     8101 taatacgttc attgaagaat caccataata acgactgata ttttcgccaa atcctcctga
     8161 caaactacca tcttctaatg tgatgactaa ttcgtgtgtt tcttttaatt tatctaatgt
     8221 ttctacgtca ttttctgtga tcgttcctgg attgatcaac gttgcttcga ttcctttttt
     8281 tctaaaagct tttactgttt cttttcctaa ttcaagaaaa ccgcctaaag ctaaaattgc
     8341 tacttttcct ccattattta ctgtttgata ggctggaata ctataatcat ttgcgatagc
     8401 tgtaccatgt tctacaccat gtgtaggaat tcggattgct acaggctgcg ctgtttgttc
     8461 gaacgcccaa tctagcatag agatcatatc ctcttttgtt gctggagcaa gatatttgat
     8521 gttcgggata cttgataagt aaggaatgtc aaatgttcct tggtgtgttc ggtcactagc
     8581 tgagatcgtt ccgccaccga ctaaaataat cactggattc tcgttgatcg ctaaatcatg
     8641 tgataattga tcatatgccc gttgcaagaa agtacttgat tggaagacga ctggacgtac
     8701 tccctgagaa gccaaagcag aagcgaaagt gatagagtgc tgttcagcga tccccacgtc
     8761 aaaataatga tctggatgtt tttcttggaa tgttttcagt ccaaaggcgc ctggtatcgc
     8821 tgcattgact gcggcaatag gagctgtact tgtttgcatt ttcttatcca aataatccat
     8881 gatgatcata ttaaatgttt ctccagaagt tacttgcttt gcctccccgg tggacagatc
     8941 aaatggtaca tgccaatgat atttcatttt attatcgatt gccggctgat aaccgcgacc
     9001 tttttccgta tggacgtgaa gtacaatcgg atgatcaata tcttttactt cttcaaataa
     9061 acggatcacc gtttccagat cgtttccatt atccacgtaa cgataatcaa atcccattgc
     9121 tttaaatagg ttattgctgc ttttaccgtt ggtttgacga agctcagcta agtttttata
     9181 taaactgcca tgattctcat caatagacat ctcattatcg tttactaata cgattagatt
     9241 ggattgtaaa acaccaccat tgttcaatcc ttcaaaagct aagccaccac ttagggagcc
     9301 gtcaccgata aatgctacca catttcctga ttctttcttc agatctctcg ctttagctaa
     9361 acctgttgct aagctgatag atgtagatgt gtgccccact gtaaaatagt catgtttact
     9421 ttcatgttgg ttggaatatc cgctcacttc acggtaatgt cctttttcaa aaccattttt
     9481 tcgaccagtt aaaagcttat gaccataaga ctgatgggaa acatcccaaa cgattttatc
     9541 ctttggtgaa tcaaatacat aatgaaacgc aatcgttaat tctactacac ctagattcgg
     9601 accaacgtgc ccacctacat cgctgtcctt ttgcaagatt ttttggcgca tctcttctgc
     9661 caactgtttc atttcctcaa cagataattt tttgacatct gcggggctat tgattgcatc
     9721 caatacatgt aatactgtac tcataataca ttctctcctc cgtactatct tcttttaatg
     9781 aaaatagatt tgttacactt tatctattct actacttaga gttgactcta agtcaatagc
     9841 attttaaaaa aatttcagag aaagcttgtt atataagtag atcttcgcac gaatcaggac
     9901 tcaaataaaa accttctaca gacaattaaa aagctttgta gaaggtctcc taattaaggt
     9961 ctcatcaaga aatatgtttt tttaataggt gaagttcctg agcaatatga tacaaatgga
    10021 tgtttgcacc ttttaatgag attggataga tctttgtctt ttcagtttct aatccatttt
    10081 gatagctagc agcatcgcac aaaaccactc tttttttcgc acatagacat tcatcaggca
    10141 attcttctaa tgaacgaaat aaaataggat ttatttcagc gatgccttct tccgcataca
    10201 aatgaaattg ggttcgataa aaaaattgtt tgactgaact ttctgaaaca acaaaaattt
    10261 ctatttcctg tctccccgct tgttcactgt tgatcaaaga aagacttttg acgaattttc
    10321 gaatgaaatt ttcattccat cttagttgga tttgattttt ctttttccac tcttccaata
    10381 tttctacaat ttttccgtac agaagttgtt ctttttctga tagaagatag atcctttccg
    10441 gtaaaaatac ttgtccatct gcccacaacg tacggacaaa ttgtaaaaag gattgcttga
    10501 aaataaaatt ttctgcatcg tcgatttcag cgatactaat aatctgtaaa atcaattcat
    10561 tcagtgatag gtcattgaga acaaaatttt tataaacaac ttcgaaatct tttcttaaca
    10621 gctccatgtt ctcattggta taatttctac tgttgaacaa agaaaaaata taatagacat
    10681 cttcttcttg taagtctaga attccttttt gctcttccaa tccttgacag actaattgaa
    10741 ataacggctt cgtttctgcg cttttcttat cttcttcttt cagtccaagg ggaaatgcct
    10801 ttcttccgat tgcgatctct attcctcgta tgataaagga cttttcatct tccgaatatc
    10861 ttcttttaaa aaaatgagtc tctacataat caatcaactc gttacaagct tcttctacat
    10921 acggattttt attttcatag ccttcccaat tgacataacg tactaaagct agcaagatgt
    10981 tccgcgagtc aaattctggg ataatgattt ctttattttt caaataatca ttttctttaa
    11041 aaaaatccaa tacgattttt ttgattgtat atactttgct caaagaaata aattcaagat
    11101 cagccagttc tgtactcgtg aatataccat taaaataata acttaaaaac tttaaaaaat
    11161 aagattgacc gtacagaaac tgtgtgagct cagaaagagc aataccatgt ttgaatacga
    11221 gtttatactt atgcttctct cttacttgtt cttttataca atattctaga cactcggcta
    11281 gactttctag atcgtgaagg atcgttggtg tagtcaccct tagagaaata gctgtttgtt
    11341 cgatgtctat ttcttggaat tcaaataatt gttcacacag atacactttt ctaaatatat
    11401 ttcgctcgat gtaattttcc agcattcctg atacacgcct ccagtttttc ttatgatttt
    11461 atttaatcat atctgtaaat cgtatttttg tttccttttt atttagaaaa gccctttcaa
    11521 aaaccaataa aaaaagaaac tgggacaaaa gtcaaacaag ctcacaattt cagcctcatt
    11581 gtaggtagct ttgagccgtt tggactttta ccccagcctc tttcaagaat ggttattcaa
    11641 agatgtcagc gtctagttct ttgactacat ctttcaacaa ttggatacca gctgcgctgt
    11701 taccaaatgg atctagtgcc gggctgaaga cacctatgcc gtaacgattt ggcacagctg
    11761 ccatcaaccc gccgcctaca ccgctttttg caggtacacc aacatggacg gaaaactctc
    11821 ctgattcatc atacaaacca gcagttgtca taattgattt gacgattgta gcactttctt
    11881 cagtaatgat ttgtttgcca ttccaaggag caattccttt atttgccagc acagcagcta
    11941 attttgctat gcctgtagcg gtgacattga gggagcattg tctgaaataa gcatcgagta
    12001 tctccggtac gtctccttcg atcatttgat tacctttcat atagtaagca agagagcgat
    12061 tgatatcacc tgttcttgat tcagaatgat agatttcttc attcaatgtg atattaggat
    12121 catcacagat ctctttcata aatgacaaga ttcgctcaaa tttttcatct gcatctttac
    12181 cggcaatcaa cgaggttgtt gcaatcgctc ctgcattaac aaaaggattc attggatgtt
    12241 tgcgatgatt gatttccata ttcatgatag aattgaaggc aaaaccggtt ggttccgtgt
    12301 tgattgtttg aaagactttt tctattccgt tgtctttgat tgctaataac aatacgggaa
    12361 cttttgacat gctttcgatg gcaaagcgca catcggcgtc tcctccctca atcagttgtt
    12421 ttgtatccaa atcataaatc gccataccta attgtttggg atccacttca gctaatgctg
    12481 gaatgtaatc cgccacttgt cctgtttgat aatagtggaa attttttcca atcaattgtt
    12541 ctaattcttt tacttccatt tgaataacca gctttcattt tttatttttc tgcatgctgg
    12601 aatttatgac ggcatgcgta gatcgtaaat ggaataacta agacgataag ataagaaaca
    12661 atcagaatcg tttcataaat cccatgctgt ccagctgcga tactagcagg tggaacaaat
    12721 gagatacaaa aagcaaaaag tgaagtcaaa agacctaaac aagcaacgat tacttttaca
    12781 acttttccgc ctggaacctg ataggctctt tcaagactgt catctttaaa aaccagcttc
    12841 aaatagcccg cgaaaaacat aaagtacaac atcaggtaga ttacaacagt caatgacatc
    12901 gctgcaagaa atgaaagatt attccccccg ccgcttaatg taaagacggc tgcccagatt
    12961 gttgcaacga tcccatttgc gatcatcaaa tgagcaggaa caccgtgtgt gttttctttt
    13021 gcaaaatatt caggaagtaa tccttcttct gctgctacgt gaagtgcttt tgaaggagag
    13081 gttacccaag aagcaatttc tccgatgaca cctaaacaaa caagaatcgc aagaattttc
    13141 gcaatccatg caagatttgg atcaatgtgt cttaatagga attctaacga ttgaatcacc
    13201 cctgtattaa gagacaaacc atgttgaggt actgtagtag cgacggtcaa tccgccaaaa
    13261 gtatctaaaa tgattgccag aataaccaat acaaacattg ctaatggata atccttttta
    13321 gggttttcca tttcatttgc atgactggca gatgtttcta cgcccatata tgctaaaata
    13381 aaggaaacaa aaacaaccag cgtaggcaat ttcgaaaaat caggaatcaa tgttttcata
    13441 gacagtatcg tttgtagtgg agcgcctgaa gcgacataat ggatccctaa gccaaacaag
    13501 actaatgctg gcaacaagat accagctaca aaaccgatcc gagcaatcaa tgaagtgtat
    13561 ttggttcctt ttaactgtga gagtaaaata gcccagaaaa taatcaatag gattgccaat
    13621 ttcagatatt tattgttttg tatagcacca atacccgttg cataggataa tgcaccagtt
    13681 aagaaataca gcatggtcaa gtagccaact gttatctcaa accattggaa aaagattgcg
    13741 gcaaaaccga atcgttttcc caatgtgcga ctgacccaag tatagacacc gcctttttcc
    13801 catcctttca ccgttgccat ctctgctgcg cacaatgcca cagggataaa ccatagcaaa
    13861 ccgcctaata acaagaaaaa tactaagctg aagcctgagg tcgcgaatgt gggatattca
    13921 tataatgaaa cgaccattga gatggtcata gccaaaaagc caaagaacgt taattttttt
    13981 gcaggaattt tcgttttgct ttgttgcatg aatcttcctc ttttctattt gaatttgttt
    14041 caattgagtt tgttgccaaa gaagaaaaag tccaatcctt cttcttgttc ttcttttgta
    14101 gtagtgatcg ttgtaaagtg catcgccgat tttaaaatca ctgttgtcag cacataggca
    14161 tacacggcag taatgatgac accaatcact tgaatcccta agaagtacca gctgcttgga
    14221 ttcgttagta aatggctgtt agatgcaaag atccctatca gaatcgtacc tgtgaatccg
    14281 cccatgccat gaacacccca cacatcaagt gcatcatccc attgtttctt tttacgaaaa
    14341 tcaacagcag catgacagac gatacccgca atgatcccaa tcagcatggc actttttgct
    14401 tctacataac cggcacaagg cgtaatggct gccaatccag caacggatcc tgttagtacg
    14461 tcgacaaaat caaagccatt tccttttact tttgcaatga tcagccaagt aatcatcgca
    14521 aatgcaagag cgatgaatgt tgaaccaaat gcattgactg cttgttcatc tgctcgaagt
    14581 gcaccaccgg aattaaaacc gaaccagcca aaccaaagca gtcctgtacc gatagcagct
    14641 gccataagac tgctatgctg acttctttct cctaaaggaa tctttctttg accaagtgtg
    14701 aggatacatg ctaaagagcc gaatccagct gtcgtgtgga tgactgctcc accagcgaaa
    14761 tctctaaatc ccatctgatc caaaaatccg ccaccccata tccagtggca aacagggaaa
    14821 taaatcagca gattccaaaa aataacaagt ataatatagc cttttaaatt caatctccct
    14881 gcaaaagctc ctgtcatcaa aggaacggta atcacgataa acatcagttg gtaaagaaag
    14941 aacattaaaa atggaatcgt tgcaccatga aacatgttgg ggaagcttgt aacatgtctc
    15001 atcaaaaaga aatctgccgg gtttccaata acaccgccga tatcccgtcc aaaggccaat
    15061 ccgaatccac caaataccca cataatcgtt gttatcccaa cggagagaaa agattgagac
    15121 atgatcgtta gtatattatt cttccgtgct aatccgccgt aaaaaaaagc tagaccagga
    15181 gtcatcaggc aaaccattgc tgtacataaa atcatgaatg ctgtatctcc tgtattgata
    15241 ggtaatgtat tcattttttt cgcctccttt ctacgattac gagtataaaa ga
//
LOCUS       DAYYCY010000059        14815 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000059 DAYYCY010000000
VERSION     DAYYCY010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14815
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.059"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(296..1024)
                     /locus_tag="ACHAZB_001432"
     CDS             complement(296..1024)
                     /locus_tag="ACHAZB_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8614116.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(1002..2576)
                     /locus_tag="ACHAZB_001433"
     CDS             complement(1002..2576)
                     /locus_tag="ACHAZB_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFM8614117.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIEPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
     gene            2805..3743
                     /locus_tag="ACHAZB_001434"
     CDS             2805..3743
                     /locus_tag="ACHAZB_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8614118.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            3755..4666
                     /locus_tag="ACHAZB_001435"
     CDS             3755..4666
                     /locus_tag="ACHAZB_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8614119.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            4690..6162
                     /locus_tag="ACHAZB_001436"
     CDS             4690..6162
                     /locus_tag="ACHAZB_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM8614120.1"
                     /translation="MKKNMQKYLIGGASVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            6213..6824
                     /locus_tag="ACHAZB_001437"
     CDS             6213..6824
                     /locus_tag="ACHAZB_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFM8614121.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFRILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            6854..8767
                     /locus_tag="ACHAZB_001438"
     CDS             6854..8767
                     /locus_tag="ACHAZB_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HFM8614122.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            8895..11876
                     /locus_tag="ACHAZB_001439"
     CDS             8895..11876
                     /locus_tag="ACHAZB_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HFM8614123.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            11873..13534
                     /locus_tag="ACHAZB_001440"
     CDS             11873..13534
                     /locus_tag="ACHAZB_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein O-GlcNAcase"
                     /protein_id="HFM8614124.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            13574..14506
                     /locus_tag="ACHAZB_001441"
     CDS             13574..14506
                     /locus_tag="ACHAZB_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HFM8614125.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
BASE COUNT         4815 a         2333 c         2978 g         4689 t
ORIGIN      
        1 atttaaataa ctacatccat ttattctatc ttctaaaata gcctttttca tttcagttaa
       61 atagttcagc gccatttttg cactcgactt gcatagtagt tttttccata ttcctcaacc
      121 tcagcggggg ttgaagacat aaggaaaatc ctatgcgtta tgctcaactg gatctgatta
      181 aatattctaa tgtatttttc tctaccatca ttaaatggtt aatagccaaa aatcaccact
      241 tttcaatggc tccttgttgt ataagtataa ctagaagatg aacacgtctg agaaattata
      301 gaagcttttt acggtaatta ctggggctgt gtccagtagt ttttttgaag agaacagaaa
      361 aatagctcac gtgatctatt cctacttttt gactaatttc atcaatagat aagaatgtgt
      421 ctttcagtag agcacttgct cttttgattc gaaagtctag caagtaatca tgaaaggaat
      481 aacctgtttc ttttttgaag gcccgtccta aataagcggg atggtaatgg aattgtgtag
      541 ctacatcttt gagcgtaagc ttttcactat aattgttttg aacgtaatca atcacttgtt
      601 gaattgactc attaaaagaa tcatcagtta gagagttttg attagctgct gatttacgca
      661 ttttttcttc ttgtactaac ttaactaaat aaggaaaatc aatgggtttc gttatgtaac
      721 ttttaataga taaatcaatc cccttttttg catactgaaa attttggtgg gcactaatga
      781 caatactagt aaagtagcga ttggatgttt gttgcaaaga ttcgattaat tcgagtccag
      841 aaattttccc catcgtaata tctgtgataa gtaaatcaaa gcgctggttt tgcaggtttt
      901 taaatgcttc gtctccgtct gtgtagcagt gatcgataat gatgtcgggg actttcttca
      961 aaagataatg ccttatgcct tctacaattg tacaatcatc gtcaacaatt gcaattctca
     1021 acatgcatat tccccccttt ttgtattttg atagtaatcg ttgttccgac gtgtagttgg
     1081 ctttctataa caaaacgtgt atctccagga tagagatgct ttatacgtcg aaaagaattt
     1141 gagatgccga tattctcact ataaggaata tcatcattaa gtttttgttg aatatgctcc
     1201 agcttgtctt ggtccattcc tttcccattt tcaacgattt ttaacacata atggttggtt
     1261 gtttcagttg catagacagt caaacgattt tccttttttg atgaatcgaa gccgtgttta
     1321 atataatttt caacaaatgg ctgtaaagaa agttttggta acattaattg atcaatatga
     1381 tcaatcagct gatagtcaaa gttatgtcga taacgacgtt gatgtagttg caaggagagg
     1441 tcgatattaa ttaattcctc tttcaaatca attatgtcgg gctgatatgt attattgcga
     1501 tacaacttgc tcaataaata tatactgtcg ctgatttctt cgtcatcatt caatatggca
     1561 gtcattcgaa taaattccaa attattaaat agaaaatgag gctcgatttg atattgaagt
     1621 gctgcatact ttgcttcttg cttcaaccaa ttattttcat aaagtgtttt ttgatatgta
     1681 gcattatcta aataaaactg attgatatgc atagcgattt ctttcaaatc accgactttg
     1741 ttttcgatat tgattgactt gtagtctcct gtagatgaat gatttaagga agtaatgatt
     1801 tcctgatact gctggatata gttacgaaaa cttctattta ataattgatg aatagcaaga
     1861 ataaacaaac tcattaataa gaaaatcatg attgccagac ctaataaggt ttgtgacgaa
     1921 taatttggat tttcatatac ttgtaatgtg tggttaccga tagctatctg ttgcgttttt
     1981 ttccatttag aaggcgagat gtcttcagat aaattgacgg aatcaatacc attagacaaa
     2041 gaataattgt aatgtgttgg aaagagactc ttttttagcg cgttgatcga aacgtctaga
     2101 atcaatgtag catcgggcat gtcctcacta atgaaactta atgaacgaat aaaagaaatt
     2161 ttttcgtttt ttgtaggtgc gtaggcactt aagtattttt cgtaatctaa tgagatagat
     2221 atatttttaa cttcatcatc ttgcacaagt ttttttaagt tatctactaa ataggtaggg
     2281 gagcgatcct ttgacaaaat atcttccatg tattcagcag gtgatgagag gtagtagtca
     2341 ttaaggtttt cttggaaatc ataattttga tagatctctg tcacgatacc ggagagaata
     2401 cgattatatg tttccatata gtgggtgatt tgctgaatgt tttgcaggtt ttgatttttt
     2461 tctttttcaa tcacattgat ataggaaaag aacaatgtga aacaggcaag taaagtaata
     2521 aaaaaaatca atatgtatgt aaaacgcata ataacttttg ataaatcgtt tttcattcaa
     2581 agtcctcatt ttgataatct ctaattttat gaatcattat agcacatatt acaagtgatg
     2641 taaacgcttt gcaaacaggg ctacttatga ttgtgaatac agtttccgtt taaaaactat
     2701 tctattgtag aggtttaata aactaaaaat aatgtataaa aaattaatgt aagcgattaa
     2761 ccaagtgggt ataatcaagg aaacgctgag agaggagaga caaaatgaag ctaaagaaag
     2821 ggttattagg aaatctaaaa aaaaaccgag cattgtttat tatgctttta ccaggtgcgt
     2881 tatggttatt tatattttgc tatataccaa tgtttggagt tgtaatcgct tttaaagatt
     2941 tcagaatttc atcaagggga ttctttacaa gtttgttcaa cagtccttgg gttggctggg
     3001 aaaatttcaa atatctcttt gcttcgcaag atgcttggat cattactcgc aatacggttg
     3061 tttataacgt tgtaggtatt ttgctaagta ctttttgtac agtgtcatta gcaatcattc
     3121 tgagccaaat ggcgaacaaa cggttattga aaaagatgca gacatttatg atttttccta
     3181 attttttatc atgggtagtc atcagttact ttgtttacgc atttttgagt acggataaag
     3241 gattaattaa tattttacta agcaattttg gtgtggaacc cataaattgg tatagtgaac
     3301 cgaagtactg gccttttatt attgttttta cgagtttatg gaaaggcgta ggctatggat
     3361 ccatcattta ttacgcgtca attatgggga tcgatactag ttattatgaa gctgctttag
     3421 ttgatggtgc cagtaaatgg caacagataa ggaatgtagt cttgccacat ttaagatcga
     3481 ttattgtcat tatgttgatt ttgtctgttg gtagtatttt tcgagctgat tttggattgt
     3541 tttatcaatt gccaagaaat tcgggggctc tttatgatgt gacgaatgta ttagatactt
     3601 atgtctaccg tggattaatg aatagcggag atatcggaat gtctacggca gcgggattat
     3661 atcagtcatc agttggcttt gtcttgattt tgattactaa taagatagtc aacaagttag
     3721 atcctagttc agcactattt taggaggaat caaaatgata tcaaaattaa aacggcagat
     3781 gtacttttcg ctgccagtcg aacggttttt cacaatatta ctaatcttat ttactcttag
     3841 ctgtattttt ccctttattt tcgtaatcgt catttcatta acagatgaaa aagcattaca
     3901 actcaacggt tacgaattat tgccggctca atggagcact gatgcatatc aatacttaat
     3961 acaagatggc ggacagttac ttcggtcatt aggcgttaca attatgatta cagtgattgg
     4021 aacgttgatt actgttttta tgacgggaac ctatgcttat gttttatcaa gagcctcttt
     4081 cccttaccgg aagttcttta ctttttatct tttttttacc atgttatttg cgggagggat
     4141 ggttccttct tacttagtga tgacgaagat gcttggctta aaaaatacaa tttgggcttt
     4201 gattctacct cttgctttta gcccatacaa tgtcattatt ttaaggacat tctttaagaa
     4261 gtcgattcca gaatcgatta tcgaatccgc taaaatggat ggctgtagtg agtttcgagt
     4321 ttttttccaa atcgttttgc ctttagcgat cccaggggtt gcaacaatag gtttattttc
     4381 aagcttaggt tattggaatg actggttcaa cgcattgctg tatattgaca ccaacaaatt
     4441 gattccattg caatatttgt taatgaagat tcaaaattca atggaattct tagcaaataa
     4501 taatgacatt acattagcgc agcaacaagc aattcaaaat agccttcctc aagaatcaac
     4561 acgaatggca atggtcgtag tagctacttt accgattgca attgtttatc cgttcttcca
     4621 aaaatacttt gttcaaggat tgacgatcgg tggagtgaaa gaataaaaaa gaataggaga
     4681 tgttaaaaga tgaaaaagaa catgcagaaa tatttgattg gtggtgcttc tgtactaacc
     4741 cttggcctat taggtgcttg tggtagcagt aatggcgaag caaataatga aacaggaacc
     4801 acaaaagtta cttggtatca ggttggagaa gaacccaaag attttgatga ggtcatgaca
     4861 aaagtcaacg aaaaattaaa ggaagaaatc aatgtagagt tggatatgcg gctgattccg
     4921 gacggggatt atcagcaaaa gcttggtgtg atgatcaatt caggagaaga atatgatatt
     4981 tgtttcgtca atggcacaga ttatgtgaat tatgggaaca aaggtgcttt cattagtctc
     5041 aacgatcatt tggataaaga gttgaaagat tatgcagctg aattgaatga aggatttatt
     5101 gaaggtgggg caattaatgg cgaaacttat ggattgccag tcaataacga tattgccaca
     5161 acggaatatt ggacctttaa taaagatttg gtagatcaat accaattgga tgtagagaat
     5221 gttgaaggtg tagaaacatt ggctccagta ttagaaaagt ttgcagaaaa aaatactgat
     5281 acgaatatta cgccaatcgg tatgacccgt accagtgtct gtattcctta tgacaatatt
     5341 atcggtggtg gtgtaccgct agcggttgca tttgaaggcg atacacaaac agttgttaat
     5401 acttatgaga ctccagaaat gatgacttat ttgaaggcta tgcatgagtt ttataaaaaa
     5461 ggatatattt tgaaagatgc agccactgtt acgacagatc catttcgaat tgatagtaaa
     5521 aactggtttt taggtcgtaa ctcaagtggt tatcaaccat tagcacaaaa aaaattagaa
     5581 gctctagcgg gacatggatt aattttaaaa ccagctggaa aagcttttaa aacgactgga
     5641 tttgcaagag tggcaatgca tgcggtatct gcttcaagta aacatccgga agcagccttg
     5701 aaattgttga attatgtgaa taccaatcaa gaagtagcgg atttaatggt ttatggaatt
     5761 gaaggaaaac actatgatcg caatgaagat ggttcgatta ctttaacaaa ctctgatgat
     5821 tattatcaag gcgcttgggc attggtaaat acttatctag tttcaaaaac ttcaccgatt
     5881 acagaagaaa gtaaaaaact agaagaagaa agtaaaaagt ttcaaaatga agcaccgatg
     5941 tctcctattt taggtttctc ttttgacgct tctgattttc gaacagaaat tgcaagtgta
     6001 caaaatatcg tgtcacaata tgaagtactt ttaagctcgg gaacagttga ccctgaacaa
     6061 tatgtaccag agttgttaga aaaactagaa aaagctggat tggataaaat tcaggcatct
     6121 gctcaagaac aatacgatga atggcgaaaa tctaacaaat aattttttac taaatgaaaa
     6181 gaatagacta agcagttagt ggggggattc aaatgaacga aaacaaatgg accaaaggat
     6241 ttaattgggt ttttcaattg ttgttactaa actttactta ttttatacta atttgtttag
     6301 gatttggaat tttaggtttt ttccctgcta cgatagcttt gattgctggc gttgtgaatc
     6361 gaaaaaaata tggcttgcaa gagtctgtga agttcatgag taaaatctat aagaaaaatt
     6421 ttgttagata taatctatat tgctggtcag caactttgtt gttggccggc aatattacta
     6481 cgatctggct ttgttggcag caaagcgcac cattgtttta tttattaagt acattattat
     6541 cggtaattac actgttccta gtctttgcaa tgttgaattg ggtcgtgatc aaaagtcttg
     6601 ggttgtggat gatgggagac cgactgattg actatttgct tttcacagca acgatgttgc
     6661 caacggtagt catgcaaggc ttggtgcttg gaatttattt ccgaatattg atgattttgc
     6721 ctgtgatttt tctgtttggc ggaacagctg taacggtaat attgattagt aaattaactg
     6781 aaagagcgtt agcaaagttg ccttgtcaga tacaatcgca ctaatttttt cacaaaaagg
     6841 agtatttctt tctatgaagt taactgataa acaagtagct aatatagcct ttatccagca
     6901 acttgagatt ccattgacac aaaaagatga agaacaaata gacttgattt ttactgacac
     6961 aaaggctaat gtgcgacaaa tcaagattac taaaaatgag cagcaacgaa tttgcttgtc
     7021 ttattcacat gaagacttgc tgtatcgagg attgtttatc tgccttccac gttttttagc
     7081 tcaaggagga gagatgccat acattgaaga aacctcgatc attgagacca gttattcatt
     7141 agatgcttct cgtaatggcg tgatgaataa agagactcta aaaaaattca tttcttactt
     7201 agcgttgatg gggtacaaca cattgtatat ttacacagaa gatacctatg aagtagaaga
     7261 ccttccatat tttggttacc ttcgcggagc gtatacaaaa gaagaagtga atgaaattgt
     7321 tgaatttgct tctggttttg gcatagaagt agttccatcg atccagaccc ttgctcactt
     7381 gacccaattt ttgaagtggt atgctcaaca agaactgatg gatgatcaaa atactctact
     7441 gatcggtgaa gaaaaggttt atgcggcaat cgaaaagatg attattgcct gcaaagaaat
     7501 gtatcagaca gagcgtattc atttgggaat ggatgaagcc tatcaagcag gtctgggacg
     7561 atatttaagc ctgtatgggt attgtgatcg gacaacttta atgattcaac atatggaccg
     7621 tgtgttatca attgttgata aatataagat gaaaccaatg atttggagtg atttcattta
     7681 taagctatta gatgaaaccg gtaaagatag gtattacaat cctgatttta aactcaaagc
     7741 attagcagct aaaggattac cagcaggatt aacctatgtt cattgggact atggttcaga
     7801 tgaagtttcc tcctatgaga aagtaattga taaccatttg aaattttgtg acattgatca
     7861 ttacgtttta gctagtggtg cccatatctg ggggagacta gccccgaatc atgggaaaac
     7921 agaagcaatt gtttctgcag ctgtaaaagc ttctaaaaat aaaggtgtac gttctgtgat
     7981 gttaactact tggggagatg aagggcagga aacagagcat tggcattcct taattggggc
     8041 attaaaatta gcagattatg tctattctga agaagagtgg aatgattttg aggaaatcta
     8101 cgatcgttta ttaggaacag gtagttatca attaatgtcc gaattacgat atttcgatga
     8161 agtggatacc attacaaata acaacttaca aatggcttcc gtgagtaaac tgctactttg
     8221 gcaagatccg ttagttggta tatatgactt tcatgtagcc gctcataaca aaattgccac
     8281 tagaacaatt ggtacttatt atcatgagtt agctcagaaa ttagatatga ataaaaattg
     8341 gaatccaaaa caaccaattt taacaactat tcgtcaacga tatatacttt tagctgaaat
     8401 tttagcacaa aaggcagaac taggcatcta tttacaacac tgctgtctgg tagaagacct
     8461 tgcaggatta atggaattag cgaatcggtg tgaagcacta gccaatcgag taaatgaatt
     8521 ggcatctatt catgaaataa tttggtatca aacatacaaa acctatggtt gggaagtatt
     8581 agaacaacga tatgctgcat tagctcatcg tctgaaaacc gttgcgcgtc gtattaaaaa
     8641 tcatcttcag gggcaagatg ccctagagga gcttttggaa gaacgattac cgttttgtca
     8701 atatcaagtg ccgcttgaaa tttctggctt taattatcgg caaagctcgg tgtcaggcta
     8761 caattaacga aggaatcttc ccgttgaacg gaccacttgc tcatctaatg ggaaagtggt
     8821 tcgttttcta ttcataagta gcttaccact aactctttta ttaagaagag aaaaaaagta
     8881 ggaagtgaga aaatatgtta tcggactatt atcaaaatct gtcagttata agtgtcaata
     8941 cagaaccatt acgtaattat tatattcctt atgcaagtaa agaagaagca aaacaagagt
     9001 tagaaaggga aaaaacgagc cagtttatcg atctcaatgg tacatgggat tttcagtatt
     9061 atccttctgt ccaacagtta ccggaaaatt tttttcaaga ggcctttact gaaagctatg
     9121 atagaatttc tgttcctgga gtttggcaaa cagagggatt tgacttacct cagtatatca
     9181 atgtatctta tccaattcct tttgatccgc cttatgtacc taatgatacc ccttgtggtg
     9241 tatatcggcg tcagtttcaa attgagcaaa cagatgcacg cttttactta aatctagaag
     9301 gagcagatag taattgtttt gtttggataa atagaaactt tgtaggctat gaccaagtga
     9361 gtcatagtac atctgaattt gatgtgacca agtatttaca ggttggggtt aatgaaattc
     9421 aaattctagt gatgaagtgg tgtgatggta cctactttga agatcaggat aagtggcgaa
     9481 tgagcggtct atttcgtcca gtgtatcttc ttcaacgacc aactatgcat ctgacaagtt
     9541 atcaactacg gacgttgact gatcctaaaa aaggaagcgg aacattaatg gtttcttttc
     9601 aacgatttac tcctattgaa gtatcgttac ctatttctta cgaattattc catcctgatg
     9661 gacgtctcat agcgaaaggg gagtgttact cggaacaaca aataaatttg gatgttgaag
     9721 attgcctttt ttggacagca gaaacgccta aattatatac attactttta acatgtaatg
     9781 gtgaatattt taaagaaaaa gttggctttc gagcactgac gatcaagaat cgtaagttat
     9841 tgctgaacgg aacacctatt aaattatatg gagtcaacta tcatgacgtg aatcagcaag
     9901 gcacgccagt gatgacccga gagcagttac gtgcagattt gttattaatg aaaaaattaa
     9961 atgtgaatac tgtacgaacg gcacattacc ctaaatcacc agatttttat gaattggcgg
    10021 atgagattgg tctgtatgta atcaatgaag cagatttgga gtgtcatggt gtggttaatc
    10081 tatatgatac tgaagcgaat tatcaactaa ttgctaatga tccgacattc gaaaagccga
    10141 ttatggaccg agtgatccgt tctattcttc cttatcaaaa tcgaagctct atcattatgt
    10201 ggtctatggg gaatgaatct ggttatggag taaattttga aaaagctcaa gctttagcac
    10261 ggaaattgga tgattcacgg ttgattcata atgaacgctc gattgaacca gttattgacc
    10321 aagtgaatga ttttagtaac ttggatgtta ttagtcaaat gtatccccca ctggaagatg
    10381 ttcatcgtca ttgtcaaaga agtgatactg aaaaaccttt ttttatgtgt gaatatagcc
    10441 acgctatggg caacggtcct ggagatctta aaacgtactt cgatttgatt caacagtatg
    10501 attcctttct tggcggttgt gtctgggagt ggactgatca tgcaatcaat agaggaactt
    10561 tagaaacgcc atcttattta tacggtggtg attttggtga aaaatatcac gacggcaatt
    10621 tttgcgtaga tggtttattg accccagatc ggcaattaac agtaggtgca ttggagtacc
    10681 gtaatattca ttcgcctatt caactgattg ctgtagatga ggagctgcaa gaagtaacct
    10741 tgagcaatat aactgctttt acagccggtt cagcatttaa cctttctctt gaatggtata
    10801 tagatggaaa aaaaacagtt tatgaagtaa tgaatccttt catacttgag cctggcaaaa
    10861 gttgttcatt gcccatcaca gtacctacaa cgattcatgg tgctcatacg gttgctgttt
    10921 cgctatctgt ttataaaaat gatgagcttt atggacagca acagctcttt cttaaaaaag
    10981 aaaaaatgag attgccaaga actgctcatc ataatgatgc accacaaatt tcagtaaaag
    11041 gcacagagct gataatcaaa ggagaatcct ttatttatat atatcaatta gcaaaagctg
    11101 cgttcacttc tgtaaagatc aatcaaaagg aacttttgga acgtcctact caattttcaa
    11161 tttggcgtgc accaacggat aatgatcgta acttagtaaa caaatggaaa gcagctggat
    11221 atgctgaaat gtctttagaa atcagaaaaa aagactggaa attcgtagat gatcatctcc
    11281 tgatcgaaac gtcttatcga ggagttattc caggattaca gccaattttt acaatgcaca
    11341 tacagttgaa aataaatggt gcaggcgtaa tcaaccaaaa agtatcagtg gagaaaaatc
    11401 cgatttttcc agatttccct cgctttggga tggactggcc gttgaatgat actttttcga
    11461 aggtttctta ttgtggctat gggccgtatg aaagttatcc tgataagcac tatgctgcga
    11521 tgtttggtat ttatgagatg gagattgagg aatttgatca gccatatatc aaaccacaag
    11581 aatatggtaa ccgctggcaa tgtgattggg tatcaatcaa aaatcatcaa ggatctacca
    11641 tgacggttta tggaaactct aatttttctg ctctgcctta tagtcaagct caattaaccg
    11701 aaaagtcaca ccattatgaa ttaataaaag aaaatagaac ttggttcaat ttaagttata
    11761 aacaaaatgg tatcggctca cacagttgcg gtccttattt agaagatcgt ttccgtttta
    11821 tggaagatac ttttgactgg aatatagtga ttactttcaa aggagaaaat agatgaaaga
    11881 aacaaagatc aatcaagtca aaaaaaaatt atctcgttat caagaagttg tttatcgaaa
    11941 ccattgctta aaatggtcaa aaaataagct gaatgttcgt tttaatggat tattttatcg
    12001 tttcgaacag ttagaagaag tctggaaacc ggtaacgaat ttccaatcat gtcctgtaca
    12061 gctcataaat gatccaaaag aaacatacga tttatgtata acacaccttt ataaccagca
    12121 tttgaaaaat gacggatttt atttaaaggt tactaatgac aatcaactta cgattgaatc
    12181 agcaaacctt cgagggtttc aatatgctat ggaagcctta ttagagagtt ttttttcggt
    12241 aggagatcaa ttattattac cggaattgat tctgaaacat gaaccatcag tgaaaattcg
    12301 aggaatcatt gaagggtttt atggtatccc ctggacacat gaaatgcgcc tctctctttt
    12361 ttcatatatg aaagataacc aattgaatac ttttatgtat gcccctaaag atgatgaatt
    12421 attacgtaaa aaatggcgtg aactctacga tgcgcaagaa ttagacaaat ttaaggagct
    12481 tttatctgct gctgaggcta gcaatatcga tttttggtac ttaattagtc ctggaaatga
    12541 tattgatatt acgtgtgaag aagacattca agttcttctg aaaaagttag aacaactgat
    12601 tgagcttggc gtatttcaat ttggtctatt aatggatgat attgactatc aattaaaagg
    12661 tgctgctaaa cgccgttttc gagagccagc attcgctcat gcctatttag tgaatcgagt
    12721 agaggaatat cttagcactc ggttgtccaa tcatgaatta gtcatttgtc ctacagaata
    12781 tgataatcgt catggttctc gctatttagc aacgttaagt caagaaatac cagaacaatt
    12841 accgttcttt tggactggcc caagcacgtt agcggcgtca atttctacag aagagctgca
    12901 ggaaatggct tctgtctatc aacggccgat gctgatttgg gataatattc cggtaaatga
    12961 ttatttagag gataaagaac tattgttcat gagtccatat gagaatcgaa caccgaatct
    13021 ttctaaggag agataccagg ttacaggagt agtttcgaat ccaatggctc aattagaagc
    13081 ttcaaagttt acgatcaata gtatggctaa ttacttatgg aactgtgagc gttttgatcc
    13141 tttggaaact tggacgtcag tcgttgaaaa agtagttgga agtaaactac aaccagctta
    13201 tcttacctta accaatgctt ttccaaatca ttacacaagc agtctgcttt cagatgaaga
    13261 attactgcta gccaaagacc cagaatgggt cacaaaagaa atggctgctt tagtccaagc
    13321 agtaaaatgg atccagcaag aacaacatac atctagattg agaacagaat tggagccttg
    13381 gttacaagca atcacagaaa gttggacttt ctggcaagaa tggcaattaa aaaaagatcc
    13441 aaaagaagca gaagaatggt tggctaagaa aaaaacacac cgcataggac gtgatttagt
    13501 aactgcccat ttagagcaaa ttatcaaaag ttagatatct taaaaaataa aaagcaagaa
    13561 aaaggagaac aaaatggtca agagattaat tagtgcaaac tacagtgatg tcaagaaaat
    13621 gacgtcaaaa gagttgaagc aatccatcaa agcgagtgaa ggacgggtga ttgtttgtga
    13681 aacggttgct aatactcttc cacaatctgg agaaattacg aatgcagaag ttgcgtcagc
    13741 atttggtgca gatttgattc ttttaaatgg tttcgattgt tatcgtcctg cagtttttgg
    13801 actaccaaat gtgacgattg agaaggtttt tgatctacag caaacgattg aaaacccgat
    13861 tcctaaatta aaagaactag tcggtcgtcc tgtgggaatt aatttagaac ccattgcccc
    13921 taaaacaaat atgatgagtg agaaagtaga aatcagccaa ggaagagcaa gctctgttga
    13981 aacgatacaa aaagcagaag aactaggagt cgattttatt tgctttaccg gtaatccagg
    14041 cactggagta accaatttag aaattactaa atccgtaaaa atggcaaaaa agtatttttc
    14101 gggattgatt attgctggga aaatgcatag cgccggctca gaagagccag ttgtgaataa
    14161 agaggcgatt atttcttatg cggaagcagg cgcagatatt ttactagttc cttcagtagg
    14221 gtctatccca ggctttttag agcaagacct aattgaagcg gttcggttgg caaaaaaata
    14281 tgatttgtta acgatgtctg caatcggtac tagtcaggaa agtgcccgac cagaaacatt
    14341 gcggcaatta gccattatga ataaaagttg tggtgttgat ttacaacata ttggagatgc
    14401 tggatacggg ggattggcac cagcagaaaa tattttcgaa acctcggttg caattagagg
    14461 aatgcgacat accatcaact gcgttagccg ctcgatcaat cgctaacaaa attaaataaa
    14521 aaatggttaa attgattatt taagtgggaa catacttgag atatcagcta acttctgtca
    14581 ctttaaaaaa gggactgtga cagaagtgaa aatagacaaa aaatatccga acaagagact
    14641 aggatagcag aaaaactatc gttttgcaac gaggaccaac tggaccgtag gagcaattct
    14701 cttattgttc ggatatttcg cttgttcaag gacttatgtc tgtagtgccc ttttttggta
    14761 ggaaaaatgg tagggaacca tatcattttt aatgaaaatc aagcatttca aaagt
//
LOCUS       DAYYCY010000060        14596 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000060 DAYYCY010000000
VERSION     DAYYCY010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14596
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.060"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            30..842
                     /locus_tag="ACHAZB_001442"
     CDS             30..842
                     /locus_tag="ACHAZB_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HFM8614126.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            839..1639
                     /locus_tag="ACHAZB_001443"
     CDS             839..1639
                     /locus_tag="ACHAZB_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFM8614127.1"
                     /translation="MKQRVVTAAVALVLFIPIIWIGGIAVELTAAILAVIGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1800..3068
                     /gene="rseP"
                     /locus_tag="ACHAZB_001444"
     CDS             1800..3068
                     /gene="rseP"
                     /locus_tag="ACHAZB_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HFM8614128.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAILLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPDKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3136..4845
                     /locus_tag="ACHAZB_001445"
     CDS             3136..4845
                     /locus_tag="ACHAZB_001445"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFM8614129.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTNLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELVNTLPILLN
                     QGE"
     gene            5057..9415
                     /locus_tag="ACHAZB_001446"
     CDS             5057..9415
                     /locus_tag="ACHAZB_001446"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HFM8614130.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            9560..10033
                     /gene="rimP"
                     /locus_tag="ACHAZB_001447"
     CDS             9560..10033
                     /gene="rimP"
                     /locus_tag="ACHAZB_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFM8614131.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10056..11231
                     /gene="nusA"
                     /locus_tag="ACHAZB_001448"
     CDS             10056..11231
                     /gene="nusA"
                     /locus_tag="ACHAZB_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFM8614132.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11253..11546
                     /locus_tag="ACHAZB_001449"
     CDS             11253..11546
                     /locus_tag="ACHAZB_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HFM8614133.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11543..11854
                     /locus_tag="ACHAZB_001450"
     CDS             11543..11854
                     /locus_tag="ACHAZB_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HFM8614134.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11867..14173
                     /gene="infB"
                     /locus_tag="ACHAZB_001451"
     CDS             11867..14173
                     /gene="infB"
                     /locus_tag="ACHAZB_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFM8614135.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14200..14547
                     /gene="rbfA"
                     /locus_tag="ACHAZB_001452"
     CDS             14200..14547
                     /gene="rbfA"
                     /locus_tag="ACHAZB_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFM8614136.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
BASE COUNT         4973 a         2584 c         3250 g         3789 t
ORIGIN      
        1 ggaatctttt aagtatagga ggaaaaagaa tgttacgttt ttttccgcaa aaaaacaaat
       61 atattcaaga aaaaagcgaa tttcaattta ataccgatgg accaattcct cagcacattg
      121 ccatcatcat ggacggcaat ggtcgttggg cgcaaaaccg tcgtttgcca cgagttgctg
      181 gccataaaga aggcatggaa acagtaaaga aagtcacaaa aaaagcatca aggcttggag
      241 tgaaagtgtt gacgctttat gccttttcga ctgaaaactg gaagcgacca aaagatgaag
      301 taagttttct catgcaacta cctgtcgact tctttgatac gttcgtacca gaattgatta
      361 aagaaaatgt caaagttcat gtcatgggtt atgaaaacgt attgccagaa catacgcaag
      421 atgctgtaag acgtgcaatt gagcaaacaa aaaacaatac tggaatggta ttgaattttg
      481 ctttgaatta tggaagcaga gcagagatcg tgactgctgt ccaagaaatt gccgaagaag
      541 tagcaaaagg agaaatacac gcagaagaaa tcgatgatga attgattgct aagcatttaa
      601 tgacaggttt cttgcctaaa gagttacaag atcctgagct gatgatccgt acaagtggag
      661 aagagcgaat tagtaatttt ctgttatggc agatcgcata tagtgagctg tattttacaa
      721 aagcgctttg gcctgatttt gatggtgcgc atcttgaaga agcaatcgcc tcttatcaaa
      781 atcgagaccg tcgattcggc ggtgtaaaaa aaacagaaga tgagaaaggg gatcagtcat
      841 gaaacaacgt gtggtcacag cagccgtggc gctagtcttg tttattccga ttatatggat
      901 cggaggcata gcggttgagc ttacagcagc aatcttagca gtaataggag tttacgagct
      961 gtttcggatg aaaggattga cactcttaag ttttgaagga gttttatccg ccgttggtgc
     1021 agtgttactt gttttgccaa aagaaagatg gtttttcttt cttccagaga atgtaagtac
     1081 atttatgtta ttttatctta tagttatgat tcttttagga acatcagtta tttctaaaaa
     1141 tacgtatacg attgatgaag caggttttcc tgtgcttgtc agcttgtatg tgggaatagg
     1201 tttccagaat tttgtcaatg cacgaacaga aagcttggct gttttgatat ttggtctatt
     1261 tattgtttgg gcaactgata tcggagcata tatggttggt cgacgttatg gtcagcataa
     1321 gttaagtcct gatatttcgc ccaacaaaac gattgaaggt gcgctcggtg gtattggtag
     1381 tgctgttgta gtcgcatttc tttatttatt gctataccca gcaaaagaac tatttaatca
     1441 tggtatgttt gtcatgcttc tgatgacgat tattttttca gcagtcggac aatttggtga
     1501 tttggtcgaa tctgcaatca aacgtcatta tgatgtgaaa gactcgggaa atatcttacc
     1561 aggccatggt ggaatcttag accgtttcga cagtttgctg tttgtatttc ctatcatgca
     1621 tttatttggc attatttagt aagtatacct gcgtttcttt gtgaatttca taaaagacgc
     1681 aggtttttat ttttcattat attgatacta ttggtgtctt tggtatacta tggtaaaatt
     1741 atagtgtttt atcgttgttt tataaaaaac acaaaaaaca agtaaaggag aaaaaagcta
     1801 tgaaaacgat tctgacattt atcatcgttt ttggtatatt agtgattgtt catgagtttg
     1861 gtcatttctt ctttgcgaaa cgatcaggaa tcctcgttcg cgaatttgct atcggtatgg
     1921 gtccgaaaat ttatgggcat caagcaaaag acgggacaac atatacgctt cgcctattac
     1981 ctattggcgg ttatgttcga atggcaggca atggggatga tgaaacagaa atggcaccag
     2041 gaatgcctct ttctctttta ctaaattctg acggcatcgt agagaagata aatttaagta
     2101 agaaaatcca attgacaaat gctatcccga tggaattgag ccgttatgat ttagaagatg
     2161 agctgactat cactggttat gtgaatggag acgaaacaga agtagtaacc taccctgttg
     2221 atcacgatgc gacgattatt gaaaacgacg gaacagaaat cagaattgcg ccaaaagacg
     2281 tacagttcca atcggctaaa ttgtggcagc gtatgctgac caactttgct ggacccatga
     2341 ataactttat tttggcaatc ctgttattca tcattctggc atttatgcaa ggtggtgttc
     2401 aagtgacgaa cactaatcgc gtaggagaga tcatgcctaa tggagctgcg gcagaagcag
     2461 gcttaaaaga aaatgatgag gtagtcagtg tggacggaaa agaaatccat tcatggaatg
     2521 atctgacaac agtcattacg aaaaatccgg ataaaacatt agactttaag atcgaacgag
     2581 aaggtcaggt gcaatctgtc gacgtgacac cgaaatctgt agagtcaaat ggcgaaaaag
     2641 ttggccaact tggtataaaa gccccaatga atacaggttt tatggataag atcattggag
     2701 ggacgcgtca agctttcagc ggttcattgg aaattttcaa agccttagga tcactattta
     2761 caggtttcag tttagataaa ttaggcggac ctgtcatgat gtaccaattg tcttcagaag
     2821 cagcaaatca aggtgtaaca actgttatta gtttgatggc gcttctttca atgaatctcg
     2881 gaatcgtcaa tctgcttccg attcctgcct tagatggcgg gaaattagtc ttgaatattt
     2941 ttgaagggat tcgcgggaaa ccattaagtc aagaaaaaga aggaatattg acattagctg
     3001 gctttggatt tttaatgttg ttgatggtgc tagttacatg gaacgatatt caacgattct
     3061 ttttctagaa aaagttgaca aatagggtat aataacaaac gataactatt ttgaggaaat
     3121 aaaggagtat atagaatgag acaatcaaaa atgttgattc caacattacg cgaagttcca
     3181 aatgatgcag aagtattaag ccatcaaatc ttgctaagag ctggatatat ccgccaagta
     3241 tcagcgggta tttattcata tctgccatta gcaaatcgtg tattggaaaa actgaaacgc
     3301 attatgcggg aagaatttga aaaaatcgga gcggtagaaa tgctgatgcc tgccatcttg
     3361 ccagcagaac tttgggaaga atccgggcgt tacgaaacat atggaccaaa cttgtatcgt
     3421 ttcaaagacc gcaatgatcg aaaaatgatc cttggaccaa ctcacgaaga aacttttact
     3481 gatttgatgc gtaatgaaat caattcatat aagaaattac ctttaaatct ttatcaaatc
     3541 caacctaaat atcgtgacga aaaacgacct cgcttcggtt tgcttcgcgg aagagagttt
     3601 atcatgaaag atgcgtattc tttccatgca acggaagaaa gtttggacga gacatataaa
     3661 gattacgaaa aagcctacac cgaagtattc aagcgttgtg gcttagaatt tcgctcgatt
     3721 atcggagacg gtggcgcaat gggcgggaaa gactcaaaag aattcatggc gatctcagaa
     3781 atcggggagg atacgatctg ttattcgact gaaagtgatt atgctgcaaa cttggagatg
     3841 gctacaagct actatgtatc aaaaaaatcg catgaaacac agcttgaatt ggagaaagta
     3901 gctacaccta acgtaaaatc aattgacgaa gtagcagatt tcttttctgt tgaacctcaa
     3961 aaaatcatca agtctgtatt gttcattgcg gatgaacaac ctgtccttgt tttggtacgc
     4021 ggagaccatg aagtcaatga cgtaaaactg aaaaactttt tatctgctga ctttttagaa
     4081 gaagcaacag aagaagaagc acaaaaatat ctaggtgctg agtttggttc tgtcggacca
     4141 gtcggtgttt cagatgacgt aaaagtatat gcagaccgtt atgtccaaga tttagcgaat
     4201 gccattactg gagcgaacga aacaggattc cactataaaa atgtgaatcc agaacgcgat
     4261 ttcactgtac tttcttatga agatcttcgt tttgtacaag aaggagatcc ttcaccagac
     4321 aacaatggtg ttcttgcctt tactcgagga atcgaaatcg gacatatctt caaaatcggc
     4381 actcgttaca gtgaatctat gggagcaact gtactagacg aaaatggacg agaaaaattt
     4441 gtgattatgg gctgttatgg tatcggtgta agtcgtttat tatcagcaat cgtagaacaa
     4501 cattcagatg aacaaggaat caactggcca aaaggaattg cgccatttga tttgcatatc
     4561 gtccaaatga atctaaaaga cgaataccaa acaaacctga cgaatgaact agaagaagcg
     4621 atgacaaaag ctggctatga agtactggtt gatgacagaa atgaacgcgc aggagtgaaa
     4681 ttcgcggatt ctgatttgat tggctgtcca atcagaatca ctgtcggtaa aaaagcagtg
     4741 gatggaatcg tagaagtgaa aatcaagaaa actggcgaaa tggttgaagt acgtaaagaa
     4801 gaattggtga atactttacc aatcctcttg aaccaaggcg aataataagc gttaaaacac
     4861 agaaaataaa aatagctgaa tcagctgttt ttgacaatct gatgattcgc ttttaaaatt
     4921 ggaactgcga tttgaatcgc agttccaatt ttttaatgta aaactcagaa aaaacgagta
     4981 tactaaaagg tactgaaaaa gaagaggata agatgcgatc aattgcattg tttctcatca
     5041 gaaagggcgt atatctttgt cagaaaaacg agatttattt gaaaaactgt tggaacaaat
     5101 ccaactagag gaagcagaaa aaaatcatcc actgcttact gctggtgaaa tggatagtgt
     5161 agtggtccat cgtaaatcgc gtttatggga atttacgctg catttttcca aaattttgcc
     5221 tattatgcta tatcggtcat tgactcagca tctgactatc gcttttaaag atattgcaca
     5281 agtcaaactg aatatcttag cagatgacca gacattcgat gaacagctct tgcaagatta
     5341 ctgggcccaa gctttggaaa atcagcaatg cgatacacca ctagcacagc aagtattgaa
     5401 aacgcaggta cctgtgataa aagatcgtaa agtcatcttg ccaatcgaca gtacaggagc
     5461 tatttcttat ttgaagcaac aatacttgcc attagtagaa gagttgttcg tcagttacgg
     5521 attcccaaaa tttcgtatcg aaccagaagt ggatgagcag caagcggaac gagtattgaa
     5581 actttttgaa gaacgcaaac aagaacaggc agaagccttt atgaagcaag cagctgaaag
     5641 tctagttgtc cacgaacaaa agaaaaagga aagaaaagag caatcgccag ctcttgaagg
     5701 acccatccac atccagctag gacgaaatat tccagccgat gaaccaacga caccaatgat
     5761 cagtatcgtt gaagaagaac gaagagtcac acttgagggg tacgtattcg acaaagaagt
     5821 cagagaacta cgttcaaaac gcaagatctt gactttgaaa atcacagatt atacttcatc
     5881 atttatcgtc aaaaaattct cgaacggcga aaaagatgaa caagtatttg atgcgatcag
     5941 tgtcggtagc tggttaaagg tacgaggaag tattcaagaa gatacgtttg tccgtgattt
     6001 ggtcatgaat gcgcaagata tcatagaagt caaacataca cctcgcaaag attatgctcc
     6061 tgaaggggaa aaacgagtag agcttcatgt tcatagcaat atgagtacga tggatgcaac
     6121 aaacagcatc tctgatcttg tggcacaagc agggaaatgg ggacaccgag ctatcgccat
     6181 tacagaccac ggtggtgcgc aagcctttcc ggaagcacat agtgctggaa aaaaagcagg
     6241 tgtgaaaatc ttatacggcg tggaagcgaa tgtcgtagat gatggtgtcc caattgctta
     6301 taatgatgca catgaagcat tgagtgaagc cacatatgtc gtatttgacg tggaaacgac
     6361 aggattgtct gcagtatatg acacgatcat cgagctagcc gcagtcaaaa tgtataaagg
     6421 aaatgtgatc gaaagctttg atgaatttat cgatccagga catcctttgt cacgaacaac
     6481 ggtagatctg actggtatca cggatggaat ggttcgagga tcaaagtcgg aagaagaagt
     6541 attgcggatg ttccttgaat tttcaaaaga tacaattttg gtagcccaca acgcggcttt
     6601 tgatatgggg ttcttgaata ctagctacgc aaggtatggt attcctgaag cggccaatcc
     6661 agtcattgat acgttggaat tagctagata tctgtatcct caattcaaac gtttcggatt
     6721 aggtgtgtta tctaaaaaat ttggtgtcag cttggaacag catcaccgag caatctatga
     6781 tgctgaagca actggtcact tagcatggat ctttgtgaaa gaggcaatgg acaatcataa
     6841 catgctttat catgatcagc taaatgagca tatcggagaa ggagattctt acaaaagggc
     6901 tcgacctttc catgtaacga ttttagcaaa aaatcaggca ggactgaaag atttgttcaa
     6961 actgatttca atgtcgaatg tcgaatactt cgaacgcgtc ccacgaatac cacgctccca
     7021 attgaaaaag atgcgtgaaa atctattgat cggctcagct tgtgataagg gagaaatatt
     7081 cgaagccatg atgcaaaaag gggtagaaga ggcaagaaac cgagcaaaat tctacgatta
     7141 tattgaagtt atgccaaaag cggtctatgc gcctttgatc gagcaagaat tggtaaaaaa
     7201 tgaacatgat ttggaagaaa ttatccaaaa tttggtagaa atcggaaaaa gtttggataa
     7261 aatcgttgtt gcaacaggta atgtacatta tctgaacgaa gaagatgcca tttaccgaaa
     7321 aattttgatc aattctatgg gcggagcgaa tccgttgaat cgtcatagtt tgccggatgt
     7381 ccatttccga acaaccgatg agatgctaac tgcatttcac tttttagggg aagaaacagc
     7441 gaaagagatc gtagtagaaa atacgaataa aatcgcagat atctgcgaag aagtgattcc
     7501 agtcaaagat gaactttata caccgaagat tccaggttca gaagatgaaa tatcagaatt
     7561 gagttacaca aaagcaaaac aaatgtacgg agatccgtta cctgagatca ttcaaaaacg
     7621 attaaaaaaa gagttgaatt ctattaatgg taatggcttc tcggttattt atctgatcgc
     7681 ccaaaaacta gtgcataaaa gtaatgaaga tggctatcta gtaggttcac gtggctctgt
     7741 tggctctagc tttgttgcga cgatgacagg aatcactgaa gttaatccgt tagcccctca
     7801 ttattattgt ccagagtgcc aatattccga gttttttgaa gatggtacgt atggttctgg
     7861 atttgacatg ccagaaaaac aatgtccaaa atgtggtgca cgcttaaaca aagacggtca
     7921 tgatattcca tttgagacat ttttaggatt ccatggagac aaagtgcccg atattgattt
     7981 gaacttctca ggtgattatc aagcagaagc ccataactat acgaaagtat tgtttggcga
     8041 agactatgtt taccgtgccg gaacgattgg tacagtcgcc gacaaaacag catatggcta
     8101 tgtaaaagga tacgaaaggg acaataatct gcagtttcga agtgcagaag tcgacagatt
     8161 agcaaaaggc gctactggag tcaaacggac aaccggccag catccagggg ggatcatcgt
     8221 tattcccgat tatatggatg tctatgattt tacgccgatc caatatccag cagacgatca
     8281 aaattcggaa tggaaaacga ctcattttga ttttcactcg attcacgata atgtattgaa
     8341 attagatatt ctagggcatg atgacccaac tgttattcgg atgcttcaag atttatcagg
     8401 gatcgatcca caaacgatcc ctacggatga cccggaagtg atgcggatat ttgccggtcc
     8461 tgaagtactt ggagtaagtc aggaacaaat ttattccaaa acagggacat taggtattcc
     8521 agaattcggc actcgttttg ttcgagggat gctagaagaa acgcatccta caacttttgc
     8581 agaactactg caaatttccg gactatctca tggtacagac gtatggttag gaaatgcgga
     8641 agaattgatt cgacgaggag atgcgacact ggcggaagta atcggctgtc gtgatgatat
     8701 tatggtttat ttgatccatg ctggattaga tagcgggatg gcatttaaaa tcatggaaac
     8761 tgttcgtaaa ggacaatgga acaagatccc tgatgaattg cgagaaacct atctatcggc
     8821 gatgaaagaa aataatgtgc ccgactggta tatcgattct tgttcaaaaa tcaaatatat
     8881 gtttccgaaa gcccatgctg cagcttacgt attgatggct cttcgtgtcg cttatttcaa
     8941 ggtttatttc cctattttgt attactgtgc atacttttct gtacgtgcag acgactttga
     9001 tctagtctcc atgtgcaaag gaaaagatgc tgtgaaacaa gcaatgaaag aaatcacgga
     9061 caaaggatta gatgcttccg taaaagaaaa gaatcaactg acagtgctgg agttagccaa
     9121 tgaaatgctg gaacgaggat tcaaatttgg aatgattgat ttgtataaat cggatgccgt
     9181 taactttgtt attgaaggtg atacgttgat cgcaccattt agagctgtcc caagtttggg
     9241 aaccaacgtg gcaaaacaaa tcgttgaagc aagaaaagac ggtccatttt tatccaaaga
     9301 agatcttgcc acccgcggta aagtttcaaa aacattgatc gaatatatga acgacaatgg
     9361 cgtattgaaa gatctaccag atgaaaatca attatcgtta ttcgatatgc tataagcatc
     9421 tgttttcctg tctgcttgta attttaagca gagtatggta tagttcttat aggtaatgat
     9481 actaactgtg agtgagcgga tttttcgctc actcttttta atggaattac gcacttaatt
     9541 ttttaaggag gcgaaacttt tgagtagcgt cgttgaaaca gtcacagaaa tggtgacacc
     9601 gattttagat gaacaaaatt ttgaacttgt cgaagtcgaa tttgtaaaag aaggaaaaag
     9661 ctggttctta cgagtattta ttgacaaaga aggcggtatc gatatcgaag aatgcgcctt
     9721 tgtcagtgag aaactgagtg aaaaacttga tgcagcagat ccagatccga ttcctcaagc
     9781 ttatttcctg gaggtatcct ccccgggagc agagcggcca ttgaaaaaag aaagcgacta
     9841 tgaaaaggca cttggtgatt atgtccatgt ctccttatac caaccagtag atggcgaaaa
     9901 acaatatgaa ggatttcttc aatcatttga tgctgaacag cttacattga aaatacgcat
     9961 aaaaacacga gaaaaagaat tgacttttga ccgaaaaaat attgcaaaag ctcgcttagc
    10021 tatccaattt taatcacgga gaggaatcag aaaaaatgag taaagaaatg ttaaatgcgt
    10081 tagacgcttt agaagctgaa aaagggatct caaaagaaat cgtgatcgat gctttagaag
    10141 cagcattggt ttctgcatac aaacgtcact atggccaagc acaaaacgtc gaagtggaat
    10201 ttgatcagaa aaaaggaaag atccatgtct atgcggtaaa agaagtaacg gaagaagtga
    10261 tggattcaca gttggaagtc tcattgaaag atgcactttt aatcaatcca gcatatgaga
    10321 tcggtgatac gatccgtttt gaagtaacac caaaagattt tgggcggatc gctgcgcaaa
    10381 cggctaaaca agttatttta cagcgtgtac gtgaagcaga aagaacgatt atttataacg
    10441 aattcagtgc ttatgaaaaa gacatcatgc aaggaatcgt cgaacgccag gataaacgtt
    10501 atatttatgt caatcttggt aaaattgagg cagttctttc taaacaagat caaatgccaa
    10561 atgaatttta tcaaccacat gatcgaatca aagtttatgt ttcccgagta gaaaatacgt
    10621 ctaaaggtcc gcaagtattt gttagccgca gccatccaga cctattgcgc cgcttatttg
    10681 agcaagaagt accagaagtc tatgatggaa tcgttgaaat tgtcagcatc gcccgtgaag
    10741 ctggcgatcg ttcaaaagtg gcagttcgct caaatgatcc aaatatcgat gcagtcggta
    10801 catgtgtcgg gccaaaggga caacgagttc aagcaatcgt caatgaatta aaaggcgaaa
    10861 acatggatat cgtcgaatgg aatgaagatc cagctgtatt cattgcaaat gcattgaatc
    10921 ctgcacaagt catggatgtg atcttcgatg agacaaatcc aaaggcctgc acagttgtcg
    10981 ttccagacta ccaattatcg ctagcaatcg gtaaacgcgg acaaaacgca cgtttagctg
    11041 ctaaattgac taaccataaa atcgatatca aatcagaatc tgacatgacg gaattttatg
    11101 aaaatcaaga acaacaaaaa gaaacagaag aactgcatga tgaagcaatc gttcagtcag
    11161 atttaacaga cgatgaatat gaaacaattg cattcaacaa tgaaacggtc gaagaaaaac
    11221 cagaagctta actttagtaa ggaggcaaag agatgaaaca aaggaaaatt cctttacgca
    11281 aatccgtggt ttcaggtgaa atgaaaccta aaaaagagat ggtccgaatc acccgttcaa
    11341 aagaagggga agtatcgatc gatccaactg gaaaaatgcc tggacgagga gcgtatgtct
    11401 cacttgagcc ggaagaagta caacaagctt gggataaaaa aattctcgat cgagtactcg
    11461 aaacaacatt aacagatgag ttttatcaag aacttcttga ctatgtcact caccaaaaag
    11521 cacgtaaaga gttatttgga aaatgaatac agttaatcgg caaaaagcca tgaatctcat
    11581 cggtctagcg atgcgcgcgg gaaaactaat cacgggtgaa gaactgacca ttgctgaaat
    11641 ccgcaaacaa aaagcaaaat tggtgtttgt ggcaaccgat gcaagcgaga atacaagaaa
    11701 aaagataaaa gataagagtt catattatga agttccttgc tttgagctat tttctgaagc
    11761 agaaatcacg cagatgatcg gaaaacctcg caaggtattc ggaataatgg atgccggatt
    11821 tgcgaagaag accaaggagc taattgaagg ttaggaaggt gattgcatgg gcaacaaaag
    11881 aatctatgaa ttagcaaaag aactcaaaaa atctagtaaa gacgtcgtgg aaaaagcaca
    11941 acaacttggc atcgatgtga aaaaccatat gggtgcgatt actagcaatg atgagaagaa
    12001 attgcaacaa gcgttcaaaa acccaagcac gaaacaacaa gctgcacaac aagcaagcag
    12061 aaaaccagcc aatcaacagc cgaacgatca acgtaaaaaa gaaacaacga acaatcaaca
    12121 aaaaaataaa aataaccgca gttatcaaga tcgcggtcaa gggagcggtc aagtgaatca
    12181 aggaaaaaaa ccaacaacaa accaaaataa tacgggaaat accagcagcc aaaatcgtca
    12241 aggaacgagc caaggcggca atcaaaaccg tactaacaat aacaacaaac caaacaataa
    12301 caatcgcggc aaatttaata ataataaccg taatcgttat aataaaaaag gtaaaaaagg
    12361 caaacagcaa caatcaacta aaccagctgt accaccacgc aaattccgtg aattgccaga
    12421 tgtattagag tatactgaag gtatgaacgt agcagatatt gcgaaaaaaa ttcatcgtga
    12481 gccagcagaa atcattaaaa aattatttat gcttggtgtc atggtcaatc aaaaccaagc
    12541 actggataaa gatacgattg aactgttagc gacggactat ggcatggaac cacaagaaaa
    12601 agtccaagtt gacattgctg atatcgataa attcttcgag gcagaagaag tcgatccaga
    12661 taaattagtc tcacgtccgc cagtcgtaac gattatggga cacgtagacc atgggaaaac
    12721 aactttattg gatacattac gccattcacg tgtaacaagc ggcgaagccg gcggaatcac
    12781 acagcatatc ggtgcctacc agatcgatat tgatggtaaa ccaatcactt tcttggatac
    12841 accaggacat gcggccttta caagtatgcg tgcacgtgga gcaagtatca cagatatcac
    12901 gatcttagtt gttgctgcag atgatggcgt catgccacaa acagttgaag caatcaacca
    12961 tgccaaagct gcaggcgttc caatcatcgt cgctgtaaat aaaattgata aaccaggtgc
    13021 aaaccctcaa catgttatgc aagaattaag tgagtatgag ttaattcctg aagcatgggg
    13081 tggagaaacg atctttgttg agatctcagc gaaattcggc caaaacatcg aagaattgct
    13141 ggaaatgatc ctgttagtcg cagaagtaga agatttaaaa gcagatccaa cacaacgcgc
    13201 aatcggaaca gtgatcgaag cacgtcttga taaaggtaaa ggtcctgtgt caaccttact
    13261 tgtccaagaa ggaacacttc atgttggtga cccaatcgtt gtcggtaata cttacggacg
    13321 tgtccgtgta atgacaaacg atcttggccg tcgcgataaa gaagcaggac ctgcaacgcc
    13381 agtagaaatc actggcttga atgatgtacc tcaagcaggt gaccgtttcg tagtcttcga
    13441 agacgaaaag acagcacgtg cagcgggtga agaacgcgcg aaacgtgcat tattagaaaa
    13501 ccgtgctgcc acaagccgtg tgactttgga taacttattt gaaagcctga aagaaggcga
    13561 attgaaagaa gttaacgtaa tcatcaaagc agatgtacaa ggttcagcag aagcactggc
    13621 tgcatcattg aagaagattg atgtggaagg tgtccgtgta aaaatcgtcc attcagctgt
    13681 cggagcaatc aatgaaagtg atgtaacctt agcagcagcc agcaacgcta ttatcatcgg
    13741 tttcaacgtt cgtcctacac cgcaggcaaa agtgcaagct gatactgaag aagtcgatat
    13801 tcgtcttcac cgtatcatct ataaagccat cgaagaaatc gaaactgcga tgaaagggat
    13861 gctagaccct gaatttgaag aaaaaatcac cggtcaaatg atcgttcgtg aaaccttcaa
    13921 agtttctaaa gttggtacga ttgcaggtgc ctatgtaaca gaaggatata ttcgtagaga
    13981 tagcggtgtc cgtgtaatcc gtgacggaat cgtaatctat gaaggccaac ttgcaagtct
    14041 gaaacgattc aaagatgacg tgaaagaagt caaaatgggt tatgaatgtg gtgcaatgat
    14101 tgaaaaattc aatgacatca aagtagatga cgtaattgaa ggatttatca tggaagaagt
    14161 caaaaatgac taaaaactga taaaaggagg ccaatcatta tggctaacta ccgtgaccgc
    14221 cgagtcggtc aagaaatctt aaaagaagtc aatgatattt tgcgcaaaaa ggtccgtgac
    14281 cctcgtgtgg agaacgtaac gatcacagat gtgaaagtta cgggggactt acagcaagca
    14341 acgatctatt acagcatttt atctgactta gcatcagata agaaaacagc acaagatgga
    14401 ttgaataaag catcaggatt gattcgtcga gagttagggc acaatctaag tatttataag
    14461 acacctgaat tgttctttga acttgatgaa tcagttgtct atggcaacca tatcgatgaa
    14521 ttgcttcgcg acttgaacaa agactaatca aaaactcccg caaatcctgt gaaatcaggg
    14581 gttgcgggat tttttt
//
LOCUS       DAYYCY010000061        14344 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000061 DAYYCY010000000
VERSION     DAYYCY010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.061"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..83
                     /locus_tag="ACHAZB_001453"
     CDS             <1..83
                     /locus_tag="ACHAZB_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011834665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I restriction endonuclease"
                     /protein_id="HFM8614137.1"
                     /translation="DTIVLHQRKLDLLKEQKKGFLQKMFV"
     gene            110..445
                     /locus_tag="ACHAZB_001454"
     CDS             110..445
                     /locus_tag="ACHAZB_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8614138.1"
                     /translation="MKFLNFLNYKYLNWVLYGITLFFIIYYFFTDDDIFLILAILFLV
                     SSNFSDNLIEVLTEPEIRSMTDEEQQIIKAMISENKKDVVVIKKIREYTNLGVLQAKK
                     VYEKKKKGL"
     gene            622..1572
                     /locus_tag="ACHAZB_001455"
     CDS             622..1572
                     /locus_tag="ACHAZB_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002821555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFM8614139.1"
                     /translation="MLKTYSLTKDTLLVSSESNIKNSSVVYVYGDEREYIESSEHKYD
                     FKIGNILTFDDRVAYDTMIDKNDEKSLLVSFLFPEVHEGGHLDTLTTSVVFLVFKNKL
                     IIFTKQKLKFIPELLSNMVLRDVNHFVEEVLLKIMQKDFYMMLNDLRGVKEKIDDLDS
                     EISTSGSLKPTFGDLLTLQKYMIALSTTYKANHKALDFIKKNFTTIDDIDFSTKKIID
                     DLYDTSDTMDRIILSYSQYLDKLENMINNMISYQLNMIMKTLTEISIVLTIPAIIFGF
                     WGINVHVPFEKSSYGVLAVLIISAILSLICWLLMRRKTYL"
     gene            2090..2500
                     /locus_tag="ACHAZB_001456"
                     /pseudo
     CDS             2090..2500
                     /locus_tag="ACHAZB_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017369153.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            2943..3755
                     /locus_tag="ACHAZB_001457"
                     /pseudo
     CDS             2943..3755
                     /locus_tag="ACHAZB_001457"
                     /inference="COORDINATES: protein motif:HMM:NF013311.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            3750..3914
                     /locus_tag="ACHAZB_001458"
     CDS             3750..3914
                     /locus_tag="ACHAZB_001458"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8614140.1"
                     /translation="MSYPRTDTPFITENEFAYLKANFGKYSGFLGLDLEMLLSLIMIR
                     SNNSTQILTN"
     gene            3854..3976
                     /locus_tag="ACHAZB_001459"
                     /pseudo
     CDS             3854..3976
                     /locus_tag="ACHAZB_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405294.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
     gene            complement(4538..5044)
                     /locus_tag="ACHAZB_001460"
     CDS             complement(4538..5044)
                     /locus_tag="ACHAZB_001460"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8614141.1"
                     /translation="MTYKNIVFDLDDTLYDHQLPFKRSVEKCFPTIDIQQIDNIYKRF
                     RYWSDVAFPKYTKKLISIEQLRIFRCQKTMEEFGIDSISRTEALDFQADYEYELDQIT
                     MIPEIHQLLLALHKNRIPIGILTNGPVDLQSRNSKILVPINILKSKISSLVSPLTEEL
                     QKIAMKVM"
     gene            complement(5049..5816)
                     /locus_tag="ACHAZB_001461"
     CDS             complement(5049..5816)
                     /locus_tag="ACHAZB_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM8614142.1"
                     /translation="MNKKRRLEKILDMLKIDGTITIKEIIDELDISDMTARRDLDALE
                     ADGLLTRTHGGAQLLSSKKPLEKTHIEKKSLNTKEKIDIAKKACSLIKDGDTIFIGPG
                     TTLEQLALELKGRKGYKIRVITNSLPVFLILNDSETIDLLLLGGEYREITGAFVGSMA
                     STNLKAIRFAKAFVSANAVTHNSIATYSDKEGVIQQLALNNAVEKFLLVDSTKFDRYD
                     FFNFYNLDQLDTIITDNQISPQHLEEFSQYTTILKAD"
     gene            6306..6731
                     /gene="lacA"
                     /locus_tag="ACHAZB_001462"
     CDS             6306..6731
                     /gene="lacA"
                     /locus_tag="ACHAZB_001462"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050044 - galactose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HFM8614143.1"
                     /translation="MAIVVGADLKGTRLKDVVKNFLVEEGFEVIDVTKDGQDFVDVTL
                     AVASEVNKDEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     VGAEIVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMC"
     gene            6748..7263
                     /gene="lacB"
                     /locus_tag="ACHAZB_001463"
     CDS             6748..7263
                     /gene="lacB"
                     /locus_tag="ACHAZB_001463"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_function: GO:0050044 - galactose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HFM8614144.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGHEVLDFGTYDHARTHYPI
                     YGKKVGEAVVSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTSAIYAKEELNAN
                     VIGFGGKITGGLLMNDIIEAFIEAEYKPTEENKKLIAKIEHVETHNAHQADEDFFTEF
                     LERWDRGDYHD"
     gene            7274..8206
                     /gene="lacC"
                     /locus_tag="ACHAZB_001464"
     CDS             7274..8206
                     /gene="lacC"
                     /locus_tag="ACHAZB_001464"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009024 - tagatose-6-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HFM8614145.1"
                     /translation="MILTVTLNPSVDISYPLETLKIDTVNRVKDVSKTAGGKGLNVTR
                     VLYESGDKVTATGFLGGKIGEFIESELEQSPVSPAFYKISGNTRNCIAILHEGNQTEI
                     LEQGPTISHEEAEGFLDHYSNLIKQSEVVTISGSLPSGLPNDYYEKLIQLASDEGVAV
                     VLDCSGAPLETVLKSSAKPTAIKPNNEELSQLLGKEVTKDIEELKDVLKESLFSGIEW
                     IVVSLGRNGAFAKHGDVFYKVDIPDIPVVNPVGSGDSTVAGIASALNSKKSDADLLKH
                     AMTLGMLNAQETMTGHVNMTNYETLNSQIGVKEV"
     gene            8209..9189
                     /gene="lacD"
                     /locus_tag="ACHAZB_001465"
     CDS             8209..9189
                     /gene="lacD"
                     /locus_tag="ACHAZB_001465"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFM8614146.1"
                     /translation="MVLTEQKRKSLEKISDKNGVISALAFDQRGALKRLMAQYQDTEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDKEAGLLLAYEKTGYDTSSTK
                     RLPDCLDVWSAKRIKEQGADAVKFLLYYDVDSSDELNQQKQAYIERVGSECVAEDIPF
                     FLEILAYDEEISDAGSVEYAKVKPRKVIEAMKVFSDPRFNIDVLKVEVPVNVKYVEGF
                     ADGEVVYSKAEAADFFKAQEEATNLPYIYLSAGVSAKLFQETLQFAHDSGAKFNGVLC
                     GRATWAGSVEPYIKEGEKAAREWLRTTGFENIDELNKVLVKTASPWTDKV"
     gene            9217..9534
                     /locus_tag="ACHAZB_001466"
     CDS             9217..9534
                     /locus_tag="ACHAZB_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFM8614147.1"
                     /translation="MNREEMTLLGFEIVAYAGDARSKLLEALKAAENGDFAKADSLVV
                     EAGSCIAEAHNSQTAMLAREASGEELPYSVTMMHGQDHLMTTVLLKDVIHHLIELYKR
                     GAK"
     gene            9534..11240
                     /locus_tag="ACHAZB_001467"
     CDS             9534..11240
                     /locus_tag="ACHAZB_001467"
                     /EC_number="2.7.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0019197 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase complex [Evidence IEA];
                     GO_function: GO:0015155 - lactose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactose-specific PTS transporter subunit EIIC"
                     /protein_id="HFM8614148.1"
                     /translation="MHKLIELIEKGKPFFEKISRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAYVPNAWGFHWSKDIETFLMTPYSYSMGILAFFVGGTTAKALTDSMNRDLPATNQ
                     INFLSTMLASMVGFLLMAAEPAKEGGFLTAFTGTKGLLTAFIAAFVTVNVYKVCVKNN
                     VTIRMPEEVPPNISQVFKDLIPFTVAVVLLYGFELIVKGTLGVTVAESIGTLLAPLFS
                     AADGYLGITLIFGAYAFFWFVGIHGPSIVEPAIAAITYANIDANLHLIQAGQHADKVI
                     TSGTQMFIVTMGGTGATLIVPFLFMWICKSERNRAIGRASVVPTFFGVNEPILFGAPI
                     VLNPIFFVPFIFAPIVNVWIFKFFVDTLNMNSFSANLPWVTPGPLGIVLGTNFQVLSF
                     ILAGLLVVVDTIIYYPFVKVYDEQILEEERSGKTNDALKEKVAANFNTAKADAVLGKA
                     GVAKEDVAANNNITKETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGSYGAHR
                     EMLPEFDLVILAPQVASNFDDMKAETDKLGIKLAKTEGAQYIKLTRDGQGALAFVQQQ
                     FD"
     gene            11382..12788
                     /gene="lacG"
                     /locus_tag="ACHAZB_001468"
     CDS             11382..12788
                     /gene="lacG"
                     /locus_tag="ACHAZB_001468"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HFM8614149.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYHKYPVDLELAEKYGVNGIRISIAWSRIFPTGYGEVNEKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEVLHSDGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKDKGYKGEIGVVHAL
                     PTKYPYDPENPADVRAAELEDIIHNKFILDATYLGHYSDKTMEGVNHILAENGGELDL
                     RDEDFQALEAAKDLNDFLGINYYMSDWMQAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     IAPDYVPRTDWDWIIYPEGLYDQIMRVKNDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKKHLEVLSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFDTQERY
                     PKKSAHWYKKVAETQVIE"
     gene            12811..13083
                     /locus_tag="ACHAZB_001469"
     CDS             12811..13083
                     /locus_tag="ACHAZB_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HFM8614150.1"
                     /translation="MLSDTLEKVQHLFRPGFEKVYIVSFEDCPMIPELEATPLLKCGK
                     WYVSTGKEWICHSDLELSAFEWEFLQSLDVEIRETIHFEVNYLPFQ"
     gene            13104..14003
                     /locus_tag="ACHAZB_001470"
     CDS             13104..14003
                     /locus_tag="ACHAZB_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002889672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFM8614151.1"
                     /translation="MTIELKNEYLTVQFKTLGGQLTSIKDKDGIEYLWQADPNYWNGQ
                     APILFPICGSLRNDWAIYRPQERPFFTGLIRRHGFVRKEEFILEEVNDNSVTFSIKPN
                     AEMLDNYLYQFELRVVYTLNGKSIRTEFQVTNLEMEKTMPYFIGAHPAFNSPLVEGEN
                     YEDYYIEFSEVESCSIPKSFPETGLLDLQDRTPFLENQKILDLDYSLFSHDAITLDQL
                     KSRSVTLRSRKSGKGLRVDFDDFPNLILWSTTNKSPFIALEPWSGLSTSLEEGNFLED
                     KRQVTKISPQETSRKIYDITILN"
BASE COUNT         4686 a         2363 c         2765 g         4530 t
ORIGIN      
        1 atgacactat cgttcttcat caacgtaagt tagatttgtt gaaagagcag aaaaaaggat
       61 ttctacaaaa aatgtttgtt tgatatttta aatagaaaag agagataaag tgaagttttt
      121 aaattttttg aactataaat atttaaactg ggtattatat ggtattacat tattttttat
      181 tatttattac ttttttaccg atgatgatat ttttttaatt ttagcaatac tttttcttgt
      241 ttcatctaat tttagtgata acttaattga agttcttaca gaaccagaaa ttcggtctat
      301 gacggatgaa gaacaacaga ttattaaagc tatgatttcg gaaaataaga aagacgtagt
      361 agttatcaaa aagattagag agtatacaaa tttaggtgtt ttacaagcca aaaaagtata
      421 tgaaaaaaaa aaaaagggtc tataattaca aattagataa tacattagaa gctggagaca
      481 agattgtcta gataatgtgt gagataaaat ttttggcagg ttatcataca taagacaaaa
      541 tgtttttaaa actgataacg tatttattcc acaacaatta cgcatttatt tctgagaaaa
      601 atataaagca ggaggattac aatgctgaaa acttattcct taacaaaaga tacattatta
      661 gtttcgtcag aatcaaatat caaaaattct tctgttgttt atgtttatgg tgacgaacga
      721 gaatatatcg aatcttctga acataaatat gactttaaaa taggcaatat actaacgttt
      781 gatgatcgag ttgcatatga cacgatgata gataagaacg atgaaaaaag tttgctcgta
      841 agttttttgt ttcccgaggt acatgaaggt gggcatctag ataccctgac gacatctgtt
      901 gttttcttgg tatttaaaaa taagttgata atttttacaa aacaaaaatt aaaattcatt
      961 cccgaactct tatccaatat ggttttacgt gatgtcaatc atttcgtgga ggaagtactt
     1021 ttaaagatca tgcaaaaaga tttctatatg atgcttaacg accttcgtgg agtcaaggaa
     1081 aaaattgatg atttagactc agaaatatca acgtccggtt cactcaaacc aacgtttggt
     1141 gatttattga ctcttcaaaa atacatgata gctttgtcaa caacttataa agctaaccat
     1201 aaagcgcttg atttcattaa aaaaaatttc acaactattg acgatattga ttttagtact
     1261 aaaaaaataa ttgatgattt atacgataca agtgatacaa tggatagaat cattttaagt
     1321 tacagccaat atttggacaa attggaaaat atgataaaca atatgatttc gtatcagcta
     1381 aacatgatta tgaaaaccct aaccgagata tcaattgttt tgactattcc agcaattatt
     1441 tttggctttt ggggaatcaa tgtccatgtc ccgtttgaaa agtcttctta tggtgtttta
     1501 gccgtattga ttatctctgc tatactaagt ctaatttgct ggcttttgat gcgacgcaaa
     1561 acatatttgt aaagtatttg tgatacccta atatgattta aagcagaatt tctttgttga
     1621 ctttttaaaa ggctgattcc tttcaataat ttaatagctt cagcggctgc tcagaagtca
     1681 ataaattgga ctatttttat tcagttactt aggcaacatt tgagatacga ttgcgattat
     1741 aaaaattgat ataccaacca atggctgctt ggaagactga catattagcc tcgatatcat
     1801 cgaataacta aatacagaaa aatacaataa atattatcaa actagggact atactagaaa
     1861 acatcagtac attccttaac tcagggtcta taattagaaa atcgcccctt tgaaaactga
     1921 ttaaaacagc ccccattatc tagtgactag ataatggggg ctgttttttt gttaagtgat
     1981 ataataaacc ataaaagaag tttagaaata cggtcctggt catcaataaa taaacaattt
     2041 aattaattat ttatttaatt atttaatttc agaaaggtat acatgaatca tggtgcaaca
     2101 aattgtccta cccatcaaag actccagcgt cttaaagatg gtacaagata cacttctcga
     2161 tagtttccgt gctggtcgcc gaaattacac cgtttttcaa gttggtaagg ccacgctact
     2221 acgtgtgagt gatgtcatga cattaaaaaa atcagatgtc tataatccag acggctctgt
     2281 caaaaatgca acctttattc atgataaaaa gaccggtaaa gcaaacacgt tatatttaaa
     2341 gcctgttcag caagacttgt tgcaatatca tgattggctg gcccaagaaa atatcaattc
     2401 tgagtggtta tttccctcga cagcccatta tgatcttcat atcactgaga agcagtttca
     2461 taaggtgatg gcgcatgttg gtgatctctt aggaatcaac ttggaagaca aagagaaaaa
     2521 attgcgagtc agaaaaccgt tttgagatat gaacagcatt tgccttcacg ccttagcgat
     2581 tttaaataat agccttgaaa gtgattatgg aaaagccctc tacgctcaat gcaaaattga
     2641 cgtagaaact atttttgatc gattgaaagg tgtatttggt atgtgaagaa cccatgtcag
     2701 aggcaagaac gttgcaccta ctgacattgg aatgatgctt atgagcatga atctaaccaa
     2761 attatccctt gaggctagaa gaaaagcgga agcttttcaa cacaaagcag ccaaaaataa
     2821 aaaacgcgac gaagcaatca catttatgat tattcatcgc gttttttata tttagagcta
     2881 gttatttgct agacccacta tttacagatg aaacgtttat cacctttggt tttgggcatt
     2941 tagtggaatt agcagaacca ggtcattatg acgaaaagtg gcaaaattgg aaacttgaat
     3001 ctttgccgat ttttcctgat cgatacgatt ttgaagttgc aaaagataag ggaaagcagt
     3061 ttaaaattgt tgcagaactt ctcaaaaagg caaatacaat tattgttgca acagatagcg
     3121 acagagaagg tgaaaatatc gcctggtcga ttatccataa tgcaaatgcc ttttcaaaag
     3181 ataaaacatt taaaagacta tggatcaata gcttagaaaa agatgtgatc cgaagcggtt
     3241 ttcaaaattt gcaaccagga atgaattact atccctttta tcaagaagcg caaacacgcc
     3301 aaattgccga ttggttgatc ggcatgaacg caagcccttt gtatacgtta aatttacaac
     3361 agaagggcgt acaaggtaca ttttcactag gacgtgttca aacgcccacc ttatacctta
     3421 tttttcagcg ccaggaagcc atagagaatt ttaaaaaaga accttttttc gaggtggaag
     3481 ctaagtaaaa caagggtcat ttaagggcgt tctaagcccc acacagcgtt ttaaaaccca
     3541 agaggagctt tttagctttt gtttcttcta aacaagctaa aataggcaat caagagggga
     3601 taattgctga tgttcaaacc aaagagaaga aaacgaatag tccgagttcg ttttctttaa
     3661 gtagtttgca atcaaaagtc aatcagcttt ataaagcgac agcgagccaa actttaaaag
     3721 ctatgcaagg actgtatgaa gcaaaattat tgagttatcc aagaacagat acaccattta
     3781 ttacagagaa cgaatttgct tatttaaaag cgaattttgg caaatatagc ggttttttag
     3841 gacttgatct tgaaatgttg ttatcattga taatgatacg ttcaaacaac agcacccaaa
     3901 ttttgacgaa ttagtgaaac tctatgaaaa agacgtagta aaacatgcaa catcctattc
     3961 taatcagctg gtaaagctta attaggttat ataagcttga aaataaaggg tttgtccaat
     4021 atggacaagc ctttttctta tgttttgcca cagatttgcc acacttattg tgtaagagta
     4081 tcaaggatag gtataatttg atactttgtt tttttagtga tgtgcaaata aatttctgtt
     4141 acacgactac ctctagaatg ccctacacgg tcttgaattg cttctagggg tattccttac
     4201 tctgctaaga aactaatatg cgtatgacgg aatatatggg tcgtaatatg tttattaaaa
     4261 ctaagtttat tatcaatatc aattcctgct cgttttggat taccttttcg atttaatttt
     4321 ccattttcag gtgagttgag gaaattgtta aggacactat aatgaagagg tgttccatta
     4381 tagtcgggtt tatgacgact tttggaagta aaaatccatc cattatcttt ataatcagga
     4441 ttgaatctca tatgttggtg atttaactca atttcctctt taagaatttc tatagccctt
     4501 ttagataatt ggacatttcg gtaactttgt aagtttttta cattaccttc atagctattt
     4561 tctgcagctc ctccgtgagt ggactgacta atgatgatat tttgcttttc aaaatattga
     4621 taggcaccaa tattttggag tttcgactct gtaagtcaac agggccatta gttagaatac
     4681 ctattgggat gcggttttta tgtagagcca gcagtagttg atgtatctca ggaatcatcg
     4741 taatctgatc gagttcatat tcatagtcag cttggaagtc tagagcttct gtcctagaaa
     4801 tactatcgat gccaaattct tccatagtct tctgacagcg aaaaattcta agctgctcaa
     4861 tactgatgag ttttttcgtg tatttaggaa aagcaacatc agaccaatat ctgaaacgtt
     4921 tgtaaatgtt atctatttgt tggatatcaa tcgttggaaa acatttctca acgctacgtt
     4981 taaaggggag ttgatggtca taaagagtgt catcaaggtc aaaaacaata tttttataag
     5041 tcataattct agtccgcttt taaaatagta gtgtactggc taaattcctc taagtgttga
     5101 gggctaatct ggttatctgt aatgatggta tcgagttgat ctagattata gaagttaaag
     5161 aaatcgtatc gatcgaattt agtactgtct actaataaga atttttctac agcattgttt
     5221 agggcaagtt gttgaatcac accttccttg tcactatatg tagcaataga attatgggta
     5281 acagcatttg cactaacaaa agctttggca aatctaattg cttttaaatt tgtcgaagcc
     5341 attgaaccta caaaagctcc agttatttct ctatattcac cgccaagaag caataaatca
     5401 atggtttcgc tatcatttag aatcaagaac acagggagac tatttgtaat gacacgaatt
     5461 ttataacctt tacgaccttt caattctaat gccagttgtt caagtgtagt tccaggtcca
     5521 ataaaaatag tatcgccatc tttgattaaa gagcaggctt ttttagcaat gtcaattttt
     5581 tcttttgtat ttagactttt cttctcgata tgtgtctttt caagtggctt tttagaggaa
     5641 agcaattgtg caccaccatg agtacgtgtt aaaagtccat cagcttctag agcatcaagg
     5701 tctctacggg ctgtcatatc ggaaatatct agttcatcta ttatttcttt tatggttatg
     5761 gtcccatcaa tctttaacat atctaaaatt ttttctaatc gtcgtttttt gttcatatga
     5821 agacccttct ttcataaagt aatttttttc caaagataat tctcttttaa ttgtatcata
     5881 aaagacaata ttttcaaggt aaaacaaaca atttcaaaca aaaacaaatg ttagatgatg
     5941 aaataagaac agaggattga cgtatattag cttaggtcag attttgtata agacgaaaat
     6001 aaagtaggac ctcttaatca gtaagttata gaaagtaaaa gacttttgta atacttgaat
     6061 agatatttca cgtccatttt gtgatggatt aaatgaacaa aaatgaacaa taatttaacg
     6121 gtgttatcta ttttttaaaa aaacaaataa aaaaaacaaa aaataaacaa aaaaagtgtt
     6181 gctttttgtt tgaatgcttg atattatata aacaaagaaa tgatgaaaac gttttttcga
     6241 acattttgca aaatgttttc tacttctacg tagcatttct ttctaaaatt taggaggtgg
     6301 tcaaaatggc tattgttgtt ggtgcagatc tcaaaggtac gagattaaaa gatgttgtga
     6361 aaaattttct tgtagaagaa ggttttgagg taattgatgt gactaaggac ggacaagatt
     6421 ttgttgatgt taccttggca gttgcctctg aagtaaataa agatgagcaa aacttaggta
     6481 ttgtaattga tgcttatgga gctggtccat tcatggtagc aactaaaatt aaaggtatgg
     6541 ttgcagcaga agtttcagat gaacgctcag cttatatgac acgtggacat aacaattcac
     6601 gaatgattac ggttggcgct gaaatcgtcg gtgatgagct tgctaaaaac atcgctaaag
     6661 gtttcgtcaa tggtaaatat gatggcggac gtcatcaaat ccgagtagat atgcttaaca
     6721 aaatgtgtta agaaaaggag ttaaggaatg agaatcgcaa ttggatgtga ccacattgtc
     6781 acagatgaaa aaatggcagt atcagaattc ttgaaatcaa aaggacatga ggtccttgat
     6841 ttcgggacat atgaccacgc acgtactcac taccctatct atggtaaaaa agtcggtgaa
     6901 gcagtagtaa gtggtcaagc agacttggga gtatgtatct gtggtacagg tgtcggcatt
     6961 aacaatgcag tcaataaagt tccaggtgtt cgctcagcgt tggttcgtga tatgacatca
     7021 gctatatatg caaaagaaga gttaaatgcg aatgttatcg gtttcggtgg aaagattact
     7081 ggtggtcttc ttatgaatga catcattgaa gctttcattg aagctgagta caaaccaaca
     7141 gaagaaaata aaaaattgat tgcaaaaatc gaacacgttg aaacacataa tgcacatcaa
     7201 gcagatgaag atttctttac agaattcctt gaaagatggg accgtggtga ctaccacgat
     7261 taaggtataa caaatgattc tgacagtcac actaaatcct tcagtagaca tctcttatcc
     7321 tctagaaaca ttgaaaattg atacagtgaa tagggtgaaa gatgttagta agacagctgg
     7381 tggaaaaggc ttaaatgtta ctagagtctt gtatgaatct ggtgataaag ttactgctac
     7441 aggtttctta ggtggtaaaa ttggtgaatt tattgaaagc gaattagaac aatctcctgt
     7501 tagtccagct ttttataaaa tctcaggaaa cactagaaac tgtattgcta tcttacacga
     7561 gggtaaccaa acagaaattt tggaacaagg accaacaatt tctcatgaag aagcagaagg
     7621 ctttcttgac cactatagca atcttattaa acaatcagaa gttgtgacaa tttcaggaag
     7681 tttaccttca ggacttccca acgattatta tgaaaaactt atccaacttg cttcggatga
     7741 aggagtagca gtcgttttgg attgttcagg tgcacctctt gaaacagttc taaaatcgtc
     7801 ggctaaacct actgccatca aacctaataa tgaggaactt tcacagttgc ttggaaaaga
     7861 agtaacaaaa gatattgaag aactcaaaga cgttcttaaa gagtcacttt ttagtggtat
     7921 cgaatggata gtggtctcat taggtcgaaa cggtgccttt gcaaaacatg gtgatgtttt
     7981 ctataaggta gatattcctg atatcccagt tgttaatcca gtcggatcag gggactcaac
     8041 ggtagctggt attgcatcag ctttaaatag taaaaaaagt gacgctgact tattaaaaca
     8101 tgcgatgaca ttgggtatgt taaatgctca agaaacaatg acagggcatg tgaatatgac
     8161 taattacgaa acactaaact cacaaattgg agtaaaagag gtataaaaat ggtacttaca
     8221 gaacagaaac gcaaatcttt ggaaaaaatt tcagataaaa acggtgttat ctcagctttg
     8281 gcatttgacc aacgtggtgc tttgaaacgt ttgatggcac agtatcaaga tacagaacca
     8341 actgtagctc aaatggaaga actaaaggtt ttggttgctg atgaacttac aaaatacgct
     8401 tcttcaatgt tgcttgaccc tgaatatgga cttccagcaa caaaagcatt ggataaagaa
     8461 gcaggtcttc ttcttgccta tgaaaaaaca ggttatgata catcaagtac aaaacgtttg
     8521 cctgactgct tggatgtttg gtctgcaaaa cgtatcaaag agcaaggcgc tgatgccgtt
     8581 aagttcttgc tttactatga tgtagacagc tcagatgaac ttaaccaaca aaaacaagct
     8641 tatattgaac gtgttggttc tgaatgtgtc gctgaagata ttcctttctt cttggaaatc
     8701 cttgcttatg atgaagaaat ctcagatgca ggttctgttg aatatgcaaa agtgaaacct
     8761 cgcaaagtca ttgaagcgat gaaagttttc tctgatccac gctttaacat tgatgttctt
     8821 aaagtagaag taccagttaa cgttaaatat gtcgaaggtt ttgctgatgg tgaagtagtt
     8881 tacagtaaag ctgaagctgc tgacttcttt aaagcacaag aagaagcgac taaccttcca
     8941 tacatctacc taagtgcagg tgtatctgct aaattgttcc aagaaacatt gcaatttgct
     9001 cacgactcag gtgccaagtt taatggtgtg ctttgtggac gtgctacttg ggcaggatct
     9061 gtagaacctt acatcaaaga aggtgaaaaa gcagcacgtg aatggttgcg tactactgga
     9121 ttcgaaaata ttgatgaact taacaaagtt cttgttaaaa cagctagtcc atggactgat
     9181 aaagtatagt aaaaacataa aacggaggat attgttatga acagagaaga gatgactctc
     9241 ttagggtttg aaattgttgc ttatgctgga gatgctcgct ctaaactttt agaagcgctt
     9301 aaagcggctg aaaatggtga tttcgctaag gcagatagtc ttgtagtaga agcaggaagc
     9361 tgtattgcag aggctcacaa ttctcagaca gctatgttgg ctcgagaagc ttctggggag
     9421 gaacttccat acagcgttac catgatgcat ggtcaggacc acttaatgac tactgtctta
     9481 ttaaaagatg tgattcatca cctcatcgaa ctttataaaa gaggagcaaa ataatgcata
     9541 aactcattga acttattgag aaagggaaac cgttctttga gaaaatctct cgaaacatct
     9601 atcttcgtgc tattcgtgat ggatttatcg ctggtatgcc agtcatcctt ttctcatcta
     9661 tctttatcct tattgcctat gtcccaaatg cttggggatt ccactggtca aaagacattg
     9721 agaccttctt gatgactcct tatagctatt cgatgggtat cctagcattc tttgttggtg
     9781 gtactaccgc taaagctttg acggactcaa tgaaccgtga cctacctgcg accaatcaga
     9841 ttaacttctt atctacgatg ctagcgtcta tggtagggtt ccttttgatg gcagctgagc
     9901 ctgcaaaaga aggaggcttc ttgacagcct tcacgggaac aaaaggtctt ttgacagcat
     9961 ttattgcagc ctttgtgaca gttaatgttt acaaggtctg tgtgaaaaat aatgttacta
    10021 ttcgtatgcc tgaagaggtt ccaccaaata tctctcaagt atttaaagac ttgattccat
    10081 tcactgtagc ggttgttctc ctttatggat ttgaactcat tgttaaggga actcttggtg
    10141 taaccgttgc agaatcaatc ggtacccttc ttgctcctct cttctcagct gcagatggtt
    10201 accttggaat tacacttatc tttggtgctt atgccttctt ctggtttgtt ggtatccacg
    10261 gtccttcaat tgtcgaacca gcaatcgctg ctatcactta tgccaatatc gatgccaact
    10321 tgcatcttat ccaagctgga caacatgcag acaaagttat cacttctggt actcaaatgt
    10381 ttattgttac catgggtgga acaggagcta cattgattgt tccattcttg ttcatgtgga
    10441 tttgtaaatc agaacgtaac cgtgccatcg gacgtgcctc agttgttcca acattctttg
    10501 gggttaatga gccaatctta tttggtgcac caatcgtatt gaatccgatt ttctttgtac
    10561 cgttcatttt cgctccgatt gtcaacgttt ggatctttaa attctttgtt gacacattga
    10621 acatgaactc attctctgcc aaccttccat gggttactcc tggtccgttg ggaattgtac
    10681 tcggtactaa cttccaagtt ctatcattta tcttagccgg actcttggta gttgttgata
    10741 ctatcattta ttacccattt gttaaggtat acgatgaaca aattcttgaa gaagaacgtt
    10801 ctggtaaaac aaacgatgct cttaaagaaa aagtagctgc aaacttcaat actgctaaag
    10861 cagatgccgt tcttggaaaa gcaggcgttg ctaaagaaga tgtagcagca aacaataata
    10921 tcacaaaaga aactaatgtc cttgttcttt gtgcaggtgg aggtacaagt ggcttgcttg
    10981 ccaatgcctt gaacaaagca gcagcagaat acaatgttcc agtcaaagcg gcagcgggta
    11041 gttacggtgc tcaccgtgaa atgttgccag agtttgactt ggttatcttg gctccacagg
    11101 tcgcttcaaa cttcgatgat atgaaggctg aaacggataa attgggcatt aaacttgcaa
    11161 aaactgaagg agctcaatat atcaaattga cacgtgatgg acaaggtgct cttgcatttg
    11221 ttcaacaaca gtttgattaa taaatttgta aaagttattt gagacgagat tattctttca
    11281 attccttata atattgttaa ccatatcatt gcatttcatt tccttggttt ccggaactca
    11341 gaatatcgtc tcaaatgctt tttttgaaag gatttatagt tatgactaaa acacttccta
    11401 aagattttat ttttggtggt gctacagctg cttatcaagc agaaggtgcg actcacacgg
    11461 atggtaaagg cccagttgca tgggataagt atcttgaaga caattattgg tacacagcag
    11521 agcctgcaag tgatttttac cataaatatc ctgttgattt agaattagct gaaaaatatg
    11581 gcgttaatgg tattcgtatt tcaattgcct ggtctcgtat tttcccaact ggctatggcg
    11641 aagtaaatga aaaaggtgtt gaattctacc ataatctttt tgccgagtgc cacaaacgtc
    11701 atgttgagcc attcgtgact ttgcaccact ttgacacacc agaagttctt cactcagatg
    11761 gagatttctt aaaccgcgaa aatatagaac actttgtaga ttatgccgct ttctgttttg
    11821 aagagttccc agaagtaaac tactggacaa ctttcaatga aattggccca attggagatg
    11881 gtcaatactt ggttggtaaa ttccctccag gcattcaata tgatcttgcc aaagtcttcc
    11941 aatcacacca caatatgatg gtttctcatg cacgtgctgt gaaattgtat aaagataaag
    12001 gttataaagg cgaaattggt gtagtacatg cgttgccaac caaataccct tatgatccag
    12061 aaaatccagc agatgttcgt gccgctgagt tagaagatat catccataac aaatttatct
    12121 tggatgcaac ctaccttgga cactattcag ataaaacgat ggaaggggtc aaccacatcc
    12181 tagctgaaaa tggtggagaa cttgatcttc gtgatgaaga cttccaagca cttgaagcag
    12241 ctaaagattt gaacgatttc cttggtatca actactacat gagtgattgg atgcaagcct
    12301 ttgatggtga gactgaaatc attcacaatg gtaagggtga aaaaggaagt tctaagtacc
    12361 agattaaagg tgttggtcgc cgaatcgcac cagattatgt gcctagaacg gattgggact
    12421 ggattattta tcctgaaggc ttgtatgacc aaatcatgcg agtgaaaaat gattatccaa
    12481 actacaagaa gatttacatc actgaaaacg gtcttggata caaagatgag tttgtagata
    12541 ataccgttta tgatgatggt cgtattgatt acgtgaagaa acacttggaa gttttatcag
    12601 acgcaattgc ggatggtgca aatgttaaag gttacttcat ctggtcactt atggatgtct
    12661 tctcatggtc aaacggatac gaaaaacgtt atggactatt ctatgtagac tttgacacgc
    12721 aagaacgcta tcctaagaaa tcagctcatt ggtataagaa agtagcagaa actcaagtga
    12781 tagagtaata ttacttgaaa atgtaaaaaa ttgttatccg atactttaga aaaagtacaa
    12841 cacttattta gaccaggctt tgaaaaggta tatattgtta gtttcgaaga ttgtcctatg
    12901 attcctgagc ttgaagctac cccgttgcta aaatgtggta aatggtatgt ttcaactgga
    12961 aaagagtgga tttgccattc tgatttagag ttatcagcat ttgaatggga atttttacaa
    13021 tcactcgatg tagaaatacg tgagactatt cacttcgagg taaattattt gcctttccaa
    13081 taataaaaag aaaggaatta gttgtgacta tcgaattaaa aaacgagtat cttactgttc
    13141 agtttaaaac acttggtggt caactgactt cgattaaaga caaggatggg atagagtatc
    13201 tctggcaagc tgatccaaat tattggaatg gacaggcgcc tattctattt cctatctgtg
    13261 gtagtttacg aaatgattgg gctatttata gacctcaaga aagacccttt tttacaggtc
    13321 ttattcgtag acatggtttt gttcgaaaag aagaatttat tctggaagaa gtcaatgata
    13381 atagcgtgac ctttagtatt aaaccaaatg ctgagatgct tgataattac ctatatcagt
    13441 ttgaactgag agttgtttat accttgaatg gtaaatcaat tagaaccgaa tttcaggtaa
    13501 cgaacttgga aatggaaaaa acaatgcctt actttattgg agcccatcca gcattcaata
    13561 gtcctttagt agaaggtgaa aactatgagg attattatat tgaatttagt gaggttgagt
    13621 cttgctcgat accaaagagt tttcctgaga caggattatt agatttgcaa gatcggacac
    13681 cttttttgga aaatcaaaaa attctcgatt tggactactc acttttttcc catgatgcca
    13741 ttacacttga ccagctcaag tctcgtagcg taacgcttcg gtcaaggaag tctggtaaag
    13801 gtcttcgagt agattttgat gattttccga acttgattct gtggtctaca actaataaaa
    13861 gtccattcat tgctttggaa ccttggagtg gtctctccac ttcacttgaa gaaggtaatt
    13921 tcttagaaga taaaagacag gtaacgaaaa tttcacctca agaaacttct agaaaaatct
    13981 atgatattac tatccttaat taaaatttgt tattatttga gtcttcaata agatttgaat
    14041 actcttacct tgtgatgata gattggtctt ctccgtgcca gtcttacatc gcaaggtttt
    14101 taagtttttt gtgggtctat gtcaactgta gtgggtgacg aaaagctaat atctaaagac
    14161 agtctggtag taaaaaataa gattatttac tacttcttga tttaaaataa tagtggagcc
    14221 attaatttgt ggcaaattta atttaatatt ttgcctcaaa ttagccacac tttttataga
    14281 actaagagga aaaatgaaat tttcttccta ttaatatagt tgtttgaaat cttaaatata
    14341 gcgt
//
LOCUS       DAYYCY010000062        14264 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000062 DAYYCY010000000
VERSION     DAYYCY010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14264
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.062"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(69..431)
                     /locus_tag="ACHAZB_001471"
     CDS             complement(69..431)
                     /locus_tag="ACHAZB_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8614152.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(505..885)
                     /locus_tag="ACHAZB_001472"
     CDS             complement(505..885)
                     /locus_tag="ACHAZB_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvfD/Ygs/GSP13 family RNA-binding
                     post-transcriptional regulator"
                     /protein_id="HFM8614153.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            967..2142
                     /locus_tag="ACHAZB_001473"
     CDS             967..2142
                     /locus_tag="ACHAZB_001473"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFM8614154.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2219..2803)
                     /locus_tag="ACHAZB_001474"
     CDS             complement(2219..2803)
                     /locus_tag="ACHAZB_001474"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM8614155.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3004..4002
                     /locus_tag="ACHAZB_001475"
     CDS             3004..4002
                     /locus_tag="ACHAZB_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFM8614156.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4114..4623
                     /locus_tag="ACHAZB_001476"
     CDS             4114..4623
                     /locus_tag="ACHAZB_001476"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFM8614157.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4653..5312)
                     /locus_tag="ACHAZB_001477"
     CDS             complement(4653..5312)
                     /locus_tag="ACHAZB_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HFM8614158.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5314..6078)
                     /locus_tag="ACHAZB_001478"
     CDS             complement(5314..6078)
                     /locus_tag="ACHAZB_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HFM8614159.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6086..6763)
                     /locus_tag="ACHAZB_001479"
     CDS             complement(6086..6763)
                     /locus_tag="ACHAZB_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HFM8614160.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(6800..8185)
                     /locus_tag="ACHAZB_001480"
     CDS             complement(6800..8185)
                     /locus_tag="ACHAZB_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HFM8614161.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8395..8479)
                     /gene="ffs"
                     /locus_tag="ACHAZB_001481"
     ncRNA           complement(8395..8479)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHAZB_001481"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8502..9017)
                     /gene="tadA"
                     /locus_tag="ACHAZB_001482"
     CDS             complement(8502..9017)
                     /gene="tadA"
                     /locus_tag="ACHAZB_001482"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFM8614162.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9124..9981
                     /locus_tag="ACHAZB_001483"
     CDS             9124..9981
                     /locus_tag="ACHAZB_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8614163.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10062..10397)
                     /locus_tag="ACHAZB_001484"
     CDS             complement(10062..10397)
                     /locus_tag="ACHAZB_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8614164.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10464..11834)
                     /locus_tag="ACHAZB_001485"
     CDS             complement(10464..11834)
                     /locus_tag="ACHAZB_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HFM8614165.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(11912..12448)
                     /locus_tag="ACHAZB_001486"
     CDS             complement(11912..12448)
                     /locus_tag="ACHAZB_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8614166.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            12757..14238
                     /locus_tag="ACHAZB_001487"
     CDS             12757..14238
                     /locus_tag="ACHAZB_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8614167.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         4386 a         2852 c         2332 g         4694 t
ORIGIN      
        1 tttggaccaa acaataaaaa aagaagtttg aaaaaaattc aaacttcttt gttcgcttaa
       61 tctgtatttt agaagaaagg cactccctct acttcgtaag ttccaagaac agagcgcatt
      121 tgacctggtt cttcccaaat atatccttcg cctaattctt ctctttgatt cttttgttca
      181 gtttcaaaga caaacaacgg aacattttcc tctttaatat gtgtatttgt caattccatc
      241 aaactaatct tgctaacatc gatatgaact atttctttta agaaattaag tatattcgct
      301 aaaccagtct ttccttcaga aagttccgct aacgcaaatt ccaacttgtc atcaattgaa
      361 tggaccaaaa attttttagt accatcctcc aaatgcaaca taattgtacc agcaactctt
      421 ttgcttgcca aaatttccac ctcgatcaac ttgttctatg gcttacataa aaattctagc
      481 acattttcag aaaaaataca ctttttattt cttattttct ttccgttcta gttcagataa
      541 tgcttcttct atccaaatag gtaactgctc cgcaatagtg gtaaatccga tcttacgatt
      601 tttattcgtg aaataccttt ttctgcgata tggaacagct ggatttgttt tttctaacgt
      661 tcttaaagat aaactgattt ttttggaata ttcatcaatg tcgatgatct gaactgttac
      721 tggctgtccg acttttaaca atgaatggat atttttcgta taaccggatt gtacttctga
      781 aacatgaata agtccttgcg tctcgttatc aagagaaacg aatgcgccat aaggctgtat
      841 ccctgtcact tttccttcaa ttatttgtcc aattttataa gtcatctact tccctctctt
      901 cgcatttatc attatactgt aaatagctca aaaaaagaag cgacgaaaat gactggaggt
      961 cttaagatga tcgaatttga taaacagatt tcaagaaaag atacagaaag tgtaaaatgg
     1021 gacgcaattg ctgagacata tcaaatggat gacttacttc ctttgtgggt agcagatatg
     1081 gacttcttgt ctcccggtgg ggtagctaaa gcgtttagtg aatacatcaa acatggaatc
     1141 tttggttatg ccactctgtc tgataaacta tatcaggcta tcattcattg ggaacgccaa
     1201 caccatgctg tggagttgac gaaagaaaat atcgttttta caagtggtgt gttaaccagt
     1261 ttagctgcag cagtacaaac ttttacaaaa ccaggcgatt ctattttgat tcatgaccca
     1321 gtttatcctc cattttcctc tattatagaa acaaatcaac gtcacatcgt tcgaagctct
     1381 ttgctagaaa aaaataatca tttcatcatg gacttgtctg acatggagaa aaaaatcgaa
     1441 gaaaacaatg taaaagcaat gattttatgc aatcctcaca atcctggagg gcgtgtttgg
     1501 agcaaagaag aattgaaaca gttaagcgaa ttgtgtttaa aacatcaagt gattctgttt
     1561 agtgacgaga tccatcaaga tttaacttta ttcgatcata catttacttc catgctaacg
     1621 attgatccta atttagatga tctactagtt gcttttactt ccgcaaccaa gactttcaat
     1681 ctagctgcaa tcaaaaattc aatggtattt atcaaaaatc cagagatgaa agctgatttt
     1741 gaaaaacatt tagtgatgaa ccaacagcac gaaatcaaca cttttggact gattggaacc
     1801 caagcggctt atgagacggg tggagaatgg ttaagccaac tgattcctta tctagaaaaa
     1861 aatgttcaaa cagttaatca gttttttgaa cagtatcttc cgaaagtgag aataatgaac
     1921 cctgaaggaa cttacttaat gtggttagat tttaccgcct atatttcaga cgaccaagaa
     1981 ttggagaaca ctcttgttcg gaaaggaaaa gtcgtattaa atcctggcat cacgtttggt
     2041 ccttccggtc atgggcatat gcgcttgaat attgcttgtc cagaagaaac tttactggaa
     2101 ggacttaatc gaatcaagta tgctttagag acaatcgatt aaatcattta ttcaaaataa
     2161 aaggacgaga ccttactagg tttcgtcctt tttcataatt actccgtacc ataccaaact
     2221 attctgaaat ttcaattgtt tcaatcacta catcatgtac aggtcggtct tgtggaccac
     2281 gttgtacatt tgcgatttca tctactgtgt ccatcccttc tagtacatgt ccaaagactg
     2341 tatgtcggaa atctagccaa ggagttccgc cacctttata cgcttcaatg atttctgatg
     2401 ggaatccagc accttctagt tgtccaagca tattagctgg cacatttgtg ttatttacaa
     2461 taaagaattg gcttccgttt gtgtttggac ctgaatttgc catagataaa gctccgcgaa
     2521 ggttgaacag ttcacgactg aattcatctt caaaactttc gccataaata ctttcgccac
     2581 ccattcctgt tccagttgga tcgccgcctt ggatcatgaa atcaggaata acgcggtgaa
     2641 aaatcacgcc atcataataa ccttttttgg aaagttcaat gaaattttga accgtcttcg
     2701 gtgctagttc agggaataat tgaacagtaa tgtcaccacg gtttgttttg ataacagctt
     2761 ttggtccttt ttcacttttt aaatctagtt gtgggaatgc catattgttt cctcctaata
     2821 tttgcatact tcataataat aataacataa tacctaacct ctcgtaaaaa tactagattt
     2881 gttttctttt atttttcatc actagataca ttctataact taccgtttcg atttcgaatt
     2941 caaacatttt tgagaagcat tctcaattaa attctgctat agtgtgaaaa gggaggaatt
     3001 tttatggaaa tctatgacat tacaatcatc ggtgcaggtc ctgtagggat gtttgccgct
     3061 ttttacgcag gaatgcgcca agctaaaaca aaaatcattg acagtctacc tcaacttggt
     3121 ggtcaattag caactttgta tcccgaaaaa tatatttacg atattcctgg gtatccagca
     3181 ataaaagcaa gtgaattaat cgatcaatta gaaaaacaat taactacttt taaccataca
     3241 ttccatttaa aagaagaagt tctctcgctt actcgtgaag acgaagttat cgagattact
     3301 acaaataaag gcatccatta ttccaaagca gtcattttag ctttaggaaa tggttcattc
     3361 cagccaagaa aattgaattt agataatgct gaatcatttg aaaaccacgg attggattat
     3421 ttcgtgaatg atttgatgaa gtatgctgga aaaaaagtcg ctattgctgg aggtggtgat
     3481 tctgctatcg attggacgtt gatgttagag cctattgcaa gcgaagttta cttgattcat
     3541 cgtcgcccgg aatttcgtgc gcacgaacat agtgtatcac gcttgaaatc ttcatctgtc
     3601 aacttgctta ctccctatct catcgatgga ttatcaggca ataatgggga actgacagat
     3661 atccggttaa aaaaagtgaa aagtgacgag acgattgatt tgatgattga ttctctgatc
     3721 gtcaattatg gattctcttc taatttagaa catctttctt cttggggact tgatagtacc
     3781 agaaatgcca ttactgtaaa atcagatatg tctacttcta ttccaggcgt ttatgcagct
     3841 ggtgacatct gtacctatga aggaaaagtg aaactgattg ctactgggct cggtgaagca
     3901 cctactgcag tgaacaacgc tttacactat attaatccaa aggaacgaac tcagcctggt
     3961 cacagcacaa gcttatacga taaaaatctt cgtcctagtt gattttttat atctctttgc
     4021 atttcaagtt aaacccgaaa gcagtttctt gcattttttt catgttccgt taacatgaaa
     4081 tagagaaaga aaaaataaag ggggagcaat aatatggtcg tcaaaactga gactttagaa
     4141 gaaaaggcaa gagaactatt gaaggaacgt ggcgtaactg tagaagatat cgcacaactt
     4201 gtcatgtttt tacaaaaaga ctatatttct gatttgactt tggcggattg tactgaaagt
     4261 gtcaacagcg tgctaacaaa gcgtgaagta cataacgcca ttatcactgg aatccaatta
     4321 gatatattag cagaaaagaa aaaattgatg agtccgttgc aacatattat cgaagatgac
     4381 gaaggccttt acggtatcga tgagatccta gccttgtcaa tcgtgaatgt ttacggtacg
     4441 atcggcttta cgaattatgg ttatatcgac aaggtgaaac ctggtatttt aaaagaacta
     4501 aacagccacg atggcgttca tgtccatacc ttcttagatg atatcgtcgg agcgatcgcc
     4561 gctgctgctg ccagtcgatt agcacacaaa gaaccagaaa aaagaagtcg ccttactcaa
     4621 taattcaaca aaaaaagaaa gaaaacgtct atttagacga gttctttctt ttttgtttgt
     4681 ttcaagctat tttttcctaa gaaaagaaaa acagcaaaaa ataattctaa taatacaaaa
     4741 aataagataa agctatgcat gaaaaattct tctggaagta cattgatgat tggatcagta
     4801 acgggatcga ataaccagtc atcgttgttg aaaaacgttt catgaaaaag gataaaaaag
     4861 cgatcaaatc ctatcatcat gaaaaaaccg aaaacaactg gcagtaacat gccgatttga
     4921 aacggacgaa tcaaccgcca aagccgatca ttcttttgca aatatttgat aaacaaaata
     4981 ctcgggatca aagtgatgaa aaacacagca taatccacta aaaacaaaat tttcacttca
     5041 taaaagtgat gggctccgct agcagaaact gggaaatctg gcaagctgag gatggttttg
     5101 aagggattat tgagataatt cattaggtga tcaaaatttt tcagtaacgt ctcttgatct
     5161 aaagaagtat aatccaaaat ttgcaaatgt ccgatatcaa atacgtatag tggtcgaaag
     5221 ttgatcgtca caccaactgc cagactgatc agtgtcagaa ataggctgaa caatccagca
     5281 tattccatcc actgccatct cgaatttttc atattagaag ctccattcat ccagtgattg
     5341 tttcacatag gtagctggga taggcaaccc aggtacatct tcttcttttg taaaacctga
     5401 taaaacgagc aaagtatcta tattattgcg tatcccagct tggatatctg tttcatagtt
     5461 atctccaacc atgattactt cttctttttg cagccctaat acttcaacgg ctttatccat
     5521 aatgatggct tctggttttc cgatgtagat cggcggcgtt tgggttgctg tctctacaaa
     5581 agagatgact gaccctgctc caggaagaag gccgcgttca gtaggtatat tcttatctgg
     5641 gttcgttcca atgaaagtag cacctttttg aatagcaagt gttgctttaa ctactttttc
     5701 ataggtcaga taattatcta agcctaccac tacatagtca ggagcctctt cttcccatgt
     5761 aaatccggca gctaaaatca agtcaatcaa tcctgcttct ccgataacat agactttttt
     5821 cccttttcca tctgctttca taaaatcgat tgttgccaaa gtagctgtgt agactgtctc
     5881 tggagctaca tgaatgtcaa actcatctgc tagacgatta gctactgttt ctggtgactt
     5941 agtcgtgtta ttggtaacaa acaagaaagg taattttctt ttttgcaatt cttccacaaa
     6001 tcgtttaccg gccggtatgg gttgtgtccc acgataaatc gttccgtcta aatcaattaa
     6061 atatccttta tagtccattc atccactact cttccctttt tctgatcgta aatgctgttt
     6121 ttgttttttc tacttgttta ggttgttctt gtttttttac tttttcagag gtttttgctg
     6181 actctgtttt tcgattttta ataaccggtt ttttgcgatt agatgcgact ggtccttttt
     6241 tctcatcgat atacgcacga ttatggtttt ttttcttttt tcggcgctga gttgtctttt
     6301 cttttttacc gccgattctc tcgatgacaa aatacgcaca accaaaattg caatattcgt
     6361 aaagatagtc ttccaaggaa tcgattcgct gatctggatg cgcccgtcga ttatccgctt
     6421 ggaaaaatcc ttttaggcgt agttgttcat aaccccaatc tcctacgata taatcatatc
     6481 gggctagtac atcactgtaa cgttctccaa gcttttctgc attaaatcct tcacgataat
     6541 cggacacaag ccgataatgt ctttcaccta tcaaaaaatt actgtcatct aacagtgtca
     6601 ctagctcgcc tttgactttt tctgctttag gctcctcaac gatttcttcc aaagcagctg
     6661 tcagttcttc ggtgactgtt gtttcctgtt cagctgatga ttttttctta gcaatttctg
     6721 ttttatgctc aacgttctgc tgttcatttt gttttgcaga catgcgttca ccacctatct
     6781 tttttattat accttgtttt tatttgattg ggtagtgagc tttcaagtaa tctcctacca
     6841 cacttcggat aaattcaggg aacaaaaatt cattttttcc agcaatctca atcgttggaa
     6901 aaaatggaac aaacatcaaa tgatctacgg tagtaaaagt ataattttta ttcagatcga
     6961 ttggcttgtt cttccaaaag acttgatggt taatagaatc atatgtgatt ccattataga
     7021 ccatctgccc aaaaatctta ccgcgaaatc ccattccctt cataggatag ttacgtaaaa
     7081 agttacgatt tttttctatt tctaaaacta agcggatcag atcatttcca cccaatgtga
     7141 catttaacaa gtgcatcgga tgcggcaacg cagtgtgaag ctgatcctga ttgacccgcc
     7201 cttttggaat ttcagttaaa aacagaccac tgtttaatat tgctgcttcc gtctttcctc
     7261 gttctttcac cgcctcaaag gctacttgga taaacgaatg ctcattgaag atatctagac
     7321 tcagagaaaa tggcaaatct gctacttctt tttcttttaa taattcatgc ccacgttcta
     7381 gataactatt tatttcttgt tcatcttctg taaaagctgt cattgtttcg gtagggatgg
     7441 ctttggctga atgcttaagg atttttttct cttcatcgat ggttaaatgg acttcaccga
     7501 catactgccc gtactttcca gcggctgcta tttggacatc attgattacg tgaccttctc
     7561 tgaataaatg atgggtgtgc gatcctaaaa taacatcaat tgctggtatt tcttttgcaa
     7621 tttggaaatc atcctgaatc ccgagatgac tcatcaaaac taaaacatcc acttgtgaac
     7681 gtaaatcttc gactaattct ggcaaaatat cataaggatt ccgtatgtcc cagccattcg
     7741 gattataagt aagcggaaat ggagcagtaa atgcaattag tccgattttc gtctggtgtt
     7801 tcgttgtaat gattttatat ggtttcgtcc atgccggtgg ctggagcgta tttttatcga
     7861 ataagttatc caggatgata tcaaactcag catgatcgta taaatgattc aactcttctt
     7921 tggaatttcc tacgccttca ttgtttccta tcgtcgctgc atcataacca acttcattca
     7981 tcagcaagat attttcctgt ccattcgttg cttctgacaa tggatgccaa cgatccacaa
     8041 aatcacctag atctactgca atcgttgtcg ttttttcttt cttttcgttt tctcgtttac
     8101 gttgatccaa aaaccttctg atccttggcc aattttctaa atgagaatgc agatcattcg
     8161 tatgtaaaat cactattttt tccatgtacc ctctcgcctc tctttatctt tatcatacca
     8221 taggcagctt tttcttccat tgaaacaagc tctatttcag agtttttcct gccttgaagc
     8281 aaattgtata agtacaatat tcgataaatc agtctggcta aaaaatcagg aacattcatt
     8341 tttacagtac tataaaaata aaaaaaacag acccgaaagt ctgttgtcgt ttcattataa
     8401 tcagacacat ggcggaacct gcttagtgct gcttccttcc agacctgaca cgcttcacaa
     8461 gcccaccatt gtcctagcct ttcggcaaag aatagtatac cttatttttc attcgttgac
     8521 cagtcttttt ttaatttctt ttttcgctgg cggagctttc ggaaaaagtc agtcaaaatc
     8581 atcccacatt cgtcttctaa aatgccacct tctacatatg cttggtggtt aaaccgctcg
     8641 tcctccaaaa gattcatcaa ggtaccggct gttccgcctt ttggatcata cgcgccgaaa
     8701 taaacttctg ctacacgaga caaaatcatc gcgccgctgc acataggaca aggctctaag
     8761 gtaacaaaaa gtgcagcatc ttccaagcgc cagcttccca ctttttcaca ggcttccttg
     8821 attgcaagca tttctgcatg tgtagtagca tcttgcgtgg tttctctaag gttatatcct
     8881 cttccaatca cttttccatc caatacaacc actgctccaa tcggaacttc atgaagcatt
     8941 tctgctttct ttgcttctgc caatgcaagg cgcatccatt tttctttttc tactgcatca
     9001 agatttgtat ttaccaatta cccttccctg cctttgtttt ttcttgtttt actttagcat
     9061 atgataaaaa ggctgtaact aacttttttt tctatggtaa aataaataca agaggtgaac
     9121 aagatgaaaa tggataaatt acttgctctt tacccacaag cacagaaaaa aaactctcca
     9181 gcatcagaag cggatatcct ttcttttgca attgatgatg cttttatctg gatcaaacaa
     9241 gattcattat ctcaacaaga aaccagcctt ttgaaagcgt tattccctgt aatcaacgat
     9301 caaaaaaaac acccatggta tcgctatctt tttaaagatg cttcttgtgt tcaagaaaaa
     9361 agctttcgtt tgatccagct tcacgtcgaa tcaaaaggag aattcttaaa aaaagaatgg
     9421 caagatacta ttttggaatt atttcgctat tctgctgatt tcttttttta tacagacaca
     9481 gatgcgctct tagtcgaaca aataaaccgt gattatcttg atgctactga aatcaacggt
     9541 atttttctat ctttagatgc tgattttgat acgacaacgt ctgtattcgt tggttctttc
     9601 cattcacctg gcaccgacct cgtccctttg tttgctgaag aaagaaaaat ttttttaaca
     9661 gagcaaaaca atctttatac tcacagccgt actttttcta tgcaagatgt ggctttacat
     9721 tactatacaa aagatactgt aaaagatagt aagttgatca aaagttacat agaactgatc
     9781 aaacaaaatg aaatggaagt gatcattcaa gaattatgga atgaattagg caatgtcagc
     9841 tctgctgcaa aaacactgtt tcttcatcgt aatactctaa aatatcgaat cgaaaaattt
     9901 caagaacagt ctggtttcaa tctaaaaaaa gccaacgatt tactgttttg ctatctttta
     9961 ttgcttcatg aacataatta aagatagatt aaaggggttg tgacaataat tttgtcacaa
    10021 ccccttattt cgaatgaata gtgttcaaaa gctagtttct cttacatcat tttttcaaca
    10081 aaatgcatta gccggtcttt tacatcttcg gttgaatctt tgattgaatc accataaaca
    10141 gagattttct tttttcctga tttgattttt gctaacattc ccatcaaaga atccaagtca
    10201 ctatctgaaa gaccatctac gatttctagt aatttttcat ttccatcaaa tttgtcatag
    10261 acgaattttt ttactttcgt ccgagtagtc atatgatgga tcttttccat tacttttccc
    10321 gatacaacga ctgctgtcgc tacactggca gcggctgcta cgcttaatcc gattgttact
    10381 tttgttgatg ttttcataag tatcagctcc tttttatcta tctccatcat agcatagaca
    10441 aaaaaataaa cgaactaatc cactcagtga aatctcaatc ctttagggaa aaagttcttt
    10501 actgttcctt ttacttgttt cccaaatccg acaggtatct tatctaatac tagcaaaacc
    10561 catccttgat tcccatcacg ttgaaatgtt tctcctgctg tgtaagattg ccattccttt
    10621 tgagtgatcg gcaagcaagg tatattctct atcttttttg tagcaagcgc taatgcatag
    10681 ctaggttcaa atcgattctt tttgaaatct cctaaatgca aacctgtacg tacgactttt
    10741 aaaccatcga gagaaggggc taactcagga acttcccaaa gatggtcgtt gaacactaaa
    10801 agtcttccag tagcttcata atgaaaatca tttgaaaact ccgtccagag tttttcctgc
    10861 tcttttgtca tttgcacttt actttttttt cgtgtcttct tttctttatg catctgattt
    10921 tgtccatgaa aagtcagctt tgcaacaaaa tgtccttcac cctgatcttt atgaggccat
    10981 atacgtattg ttttttccaa accagctacg cttccccatt cagaacgtcc tgaactaacg
    11041 gattgagtca aaggaatctc ctcaatcgtt actggataat tttctacaag ccagctaatg
    11101 atctcttcat tttcttctgg tgcaaacgta caagtcgaat aaatcagttg tcctttattt
    11161 tttagcattt ttattgctga agacaaaatc tcttgctgac gtttttgaca gtatagcgga
    11221 gattcttctg tccactcttt tattgcattt ggatcttttc ggaacattcc ttctcctgaa
    11281 caaggagcat caaccacgat ccgatcaaaa aagcccgaaa aatgagggac aagttctgcg
    11341 ggtgcatgat ttgtcactat tgcgttcgaa actccccatc gttctatatt ttctgataaa
    11401 atcttcgcac gtttcggaaa gatctcattc gtcactaata accctttccc cttcatttga
    11461 gcagctaatt gggttgattt accaccggga gccgcacaca aatccaaaac tttttcaccg
    11521 ggttttgctg cagcagctgt tccaacgatc attgcacttg gttcttggct atattcatac
    11581 ccagcttgat ggaggaaaga ctttccattg acagtgccaa gaaatccctc attgctgtag
    11641 ggagcgggtt gtaattcttc gctatggtaa gtttggacca tgtccaatcc tgccggtttt
    11701 agtggattcc accgaaatcc ttttttcacg cttccttgtt caagcgcaga aaaaaaatcg
    11761 gatgcttctt cgcctaaaag aagacgatat tttttgataa attgctgtgg taacgttgtc
    11821 gcttcctcct tcattccaat gtttcgttag acattttaca atctttttta cgaaaaataa
    11881 aggtggctga tctacttttt catgttgctg cctaaggtga ctgttccttt aagatatttt
    11941 ccaagtattc atccgtcacc tcgtttttat cccaaacagc gttcatcatt tcaagaaaat
    12001 cttgagaaga atcatctgag cgcaaacttc ccaatgcatt aagccatacc aattcaagcg
    12061 acttttcttc tttgatcttc gtgtgacctt tgacatatga gaaaaatggc cgcttaaata
    12121 gcgaatccat gtctaaatca gattttgcaa tgatttcacg aatactttca tggatagaaa
    12181 aaaaatcttt caataagtag acaatgctta aaacatcatc ggaactgcat tcagctaagt
    12241 agttttcaaa ataaacagtg atttcttcta atatcttctc ttttaacccg ctgattgatt
    12301 tgaaatgtgt ataaatcggg tatgtagaac aaccgcagaa ctctgctaac tctcttgcag
    12361 taattgactt cattccatat tcttccacgt ataccatact tttgaacagc aaatattctt
    12421 tactataatt tgttactctc cccatcattc ttacctccgt ctcttttcat ctacccataa
    12481 ataaataaca taaataattc taaaaatatt attgataatt atcaaaacat atttagttat
    12541 atccattaaa aatcaatttt tccgccttaa aaacaagaat atcttgacaa aataccctag
    12601 gaaataaaca atagaataaa caaggtaaca aaaaaattaa ataacgttat ataaaaaaga
    12661 aggcgacact ctgtgagaat aacacagact gaaatgactt tccactcaca gtttttgtct
    12721 aactatatac aaaaacagaa taaaaaggag gtatttatgc taattgaagc aagaaaaaaa
    12781 gagttacttg ttctacaaat ggtcattcaa aaaaataatt gttctttaca agaattatcc
    12841 aatgacttac atattccaaa acggacaatc aaagaactga tccgaaaaat aaatgtatca
    12901 ctcaagcaat ttttattgat tgatgaattt atttattcta atcataaagg agagatccaa
    12961 accaaagaaa ccgatcaaac taaaaagtta gctgtttttt ctaagttaaa attatgctac
    13021 ctaaaagaat ccaatcgttt taactatttg atgttactaa tcaattatcc acaaactgct
    13081 gtaccaaaaa aatatttact tgaacaactt tatatatcgc cctcctatct cgaaaaactg
    13141 acaaaacagc tcaatcattc cttaaaaaaa ttccatttac ggatcctatc tgtaaaaaat
    13201 tgctgtactc ttgaaggaaa tgagttatct atccgtctct atctttactt ttttctttca
    13261 gatacatttg gaggtaacga ttggccattt tcctcacttg aattaaatgc actaaaaatg
    13321 aacagtacag atgagcaaat agaaaaaatt tcaaaacaag aaaaaaatat ctacttgctg
    13381 ttaactttat tcatattacg aaacaaacaa caggcatatt ttccaaaaac ttcttctgaa
    13441 atcactgaat tgatggactt attgcagcat acacatgatt attcagaata ctttacttct
    13501 ttttcagtga gtcgcccttg tgaagagaca gaaacagaga gagcctactt taattatttt
    13561 ttctattgtt tttttccaaa tcaaatctct tttcgacaaa aacaggagat tggtttttta
    13621 ttttccacaa gcaaaaacct tatttgtcga caagtcggtc aattgatgac ttacttaact
    13681 caacagtttc ctctcaacca tttagaaaat aaaataccaa tttattttta ttttcttgtg
    13741 ttggcagttg tttttttcga actgttgggt gaatccagca gtagtttttc tacactttgc
    13801 tttccagaag ccacgtccaa tcattctagt tcttttatac aggaaaagaa attaagagaa
    13861 aaaatcgata caattttttc aaaaaatggg gaaagaaacc aaatcagtca ataccttagt
    13921 caattgatcc aacacttact acaaacagaa acgaaagagc agttactcat ccatttgcag
    13981 ataaccaaac agtttacagg aaattatcat cttcaaagcc atttgcagca gttatttcgc
    14041 aaagatgctg ttaaaattac cgcatattca gaacatgctg atttgatcat tacagatcag
    14101 catgagaaaa tttcagatga aacggagact ttctatctgg atacaacgca caatacagtg
    14161 ttatttggca agttaataaa agcggttcat gaactgtata tgaaaaaagg ttatgagaac
    14221 aaaacaaaga ggatgtgaca aaattcttgt cacatcctct tatt
//
LOCUS       DAYYCY010000063        13909 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000063 DAYYCY010000000
VERSION     DAYYCY010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13909
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.063"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(17..1246)
                     /gene="hflX"
                     /locus_tag="ACHAZB_001488"
     CDS             complement(17..1246)
                     /gene="hflX"
                     /locus_tag="ACHAZB_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFM8614168.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="ACHAZB_001489"
     CDS             complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="ACHAZB_001489"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFM8614169.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2169..2906)
                     /locus_tag="ACHAZB_001490"
     CDS             complement(2169..2906)
                     /locus_tag="ACHAZB_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFM8614170.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(2997..3524)
                     /locus_tag="ACHAZB_001491"
     CDS             complement(2997..3524)
                     /locus_tag="ACHAZB_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8614171.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3704..4297
                     /gene="clpP"
                     /locus_tag="ACHAZB_001492"
     CDS             3704..4297
                     /gene="clpP"
                     /locus_tag="ACHAZB_001492"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFM8614172.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4401..4886)
                     /locus_tag="ACHAZB_001493"
     CDS             complement(4401..4886)
                     /locus_tag="ACHAZB_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFM8614173.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(5039..5236)
                     /locus_tag="ACHAZB_001494"
     CDS             complement(5039..5236)
                     /locus_tag="ACHAZB_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8614174.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="ACHAZB_001495"
     CDS             complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="ACHAZB_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HFM8614175.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="ACHAZB_001496"
     CDS             complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="ACHAZB_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HFM8614176.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            9211..9423
                     /locus_tag="ACHAZB_001497"
     CDS             9211..9423
                     /locus_tag="ACHAZB_001497"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit epsilon"
                     /protein_id="HFM8614177.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9425..11104
                     /locus_tag="ACHAZB_001498"
     CDS             9425..11104
                     /locus_tag="ACHAZB_001498"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFM8614178.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(11358..11558)
                     /locus_tag="ACHAZB_001499"
     CDS             complement(11358..11558)
                     /locus_tag="ACHAZB_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8614179.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(11708..11851)
                     /locus_tag="ACHAZB_001500"
                     /pseudo
     CDS             complement(11708..11851)
                     /locus_tag="ACHAZB_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
     gene            complement(12086..12748)
                     /locus_tag="ACHAZB_001501"
     CDS             complement(12086..12748)
                     /locus_tag="ACHAZB_001501"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8614180.1"
                     /translation="MNTIESQFDKVAEDYDFVNELLNDYSFFVSNMSPKKGRALDIGC
                     GSGLLVEKLASYYDEVVGIDISNQMLDLAKSKRQLTNTVYLNMNAEQLNFNEKFDFIV
                     SRTTFHHLDDIASVIQQMKELLNEEGRIVILDNVSEVETPPTYVYKLGAIQEFLPHCF
                     KFGIKNAIRIYNHNTSKSWLEHLASDKYLSEQNYYDLYEKLLPGCQFHKMGWAMGVVW
                     TK"
     gene            13140..13199
                     /locus_tag="ACHAZB_001502"
     CDS             13140..13199
                     /locus_tag="ACHAZB_001502"
                     /inference="COORDINATES: protein motif:HMM:NF019858.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin resistance leader peptide"
                     /protein_id="HFM8614181.1"
                     /translation="MGTFSIFVINKVRYQPNQN"
     gene            13257..>13909
                     /gene="erm"
                     /locus_tag="ACHAZB_001503"
     CDS             13257..>13909
                     /gene="erm"
                     /locus_tag="ACHAZB_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S ribosomal RNA methyltransferase Erm"
                     /protein_id="HFM8614182.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALK"
BASE COUNT         4157 a         2922 c         2368 g         4462 t
ORIGIN      
        1 ttaaaaaaat aaaattttat tggttttttc ttaaccattt tgaattggtt tttgcaaacc
       61 ctttaacttc atattcgttt gtttcttcca tgtacgcttc gtccaaaacc attgtttgtc
      121 ttctcaactc gcttaatttc tgtccttcgt tgcttggcac agaaagtgta taaggggtca
      181 ttagttccat cagctgacgt ttgattgctt cgatcaacgc catcttacct tctttcgtct
      241 gagtagaaat caatacattt gggaatagcg taggcgtgaa catcgctggg tcgatctggt
      301 ctgccttatt gtaaacagtc agtatcggca tttctttcat attcagttca tccatcaatt
      361 ttaagacagt ctgctcctgt aaaatacgat ctggagaact agcatcgact acatgtagca
      421 aaagatccat attttgactt tcttctaatg tagaatggaa agcatcgatc agttgcgtag
      481 gcaggtcttg gatgaacccg acggtatccg ttacagtaat ctcaaatcct tcagcaaaac
      541 gccatctctt agtcaatgga tctaacgttg caaacaattg atctttcgaa taagtatccg
      601 cttgtgtcaa tagattcaag atagttgact tcccggcatt cgtgtagcca ataagtccaa
      661 tctgaaaaat ctcgctattt tttctttttt ggcgattgcg ctctcgatga gcttctactt
      721 ctttcaattc tttcttaaca gcaaaaattt tattccgtat atgtcggcgg tctgtttcta
      781 actttgtttc acctggccct cttgttccga tcccaccgcc aagacgagat aatgttttcc
      841 cttgccctac taaacgaggc agcaggtatt ctaactgagc aagttcaacc tgcagcttcc
      901 cttctttcga acgagcacgc atcgcaaaaa tatctaagat aagctgcacg cgatcaatta
      961 ccggaagacc aacagcatcg gtaatcagtt ggctctgtct cggagttagc tcatgattaa
     1021 agatgatcaa atccgcttca tgcgcatccg cttgttgaac taattcttgt aatttccctt
     1081 taccgataat cgtttgccga tccacttgtg gtctcttttg tgtaattgtg aatacgactt
     1141 ctcctgatgc ggtattcgtc agctgaacta gctcttccat tgactctgaa aagtaacgct
     1201 ggtttgcttc ggtttctacc cctacgatga tcactttttc ttccatttta ttccgcctcc
     1261 agccattttt tgattgtttg ctcaatctga cttttttgat ttggctcact tataagatca
     1321 aaccactgtg catccaaacg gttacgaaac cacgtcaact gacgtttcgc gtatcttctc
     1381 gaatgtagtt tgacttgttc tgttgcttct tccaatgttt ctttcccttc aaaataaggg
     1441 aaaaattctt tatatccaat accttgactg gcctggactt ctccaagctc atacacctta
     1501 cgcgcttctg ctactaaacc ttctgctagc atcaaatcga ctctttgatt gattcgttca
     1561 taaagaatac tacgcttcgt atctaatcca atcatgaggt aatcatacaa ttgctttggc
     1621 tcttctttag gtgccgtgat actatgtcct gtcgtctcta aaacttccag agctcttatc
     1681 actttgcgtt gattgttcca gtggattttc tcagcagctg ccgggtcttt ttctttcaac
     1741 tgattccata atgcttggga accaattgtt tcagctaacg tttcatactt ttttctaatc
     1801 tcaggattcg cttcttctgt cgctcctaac tgataatcat atagaaggga ttggatatag
     1861 agacccgtac caccagctat gataggtagg tgccctcggt tcgcaatctc ggtaatcaat
     1921 tgtcgacctg aatgctgaaa atcagcggct gagtaacgtt gagtaggttc gatcacatca
     1981 atcaaatgat gcggaacatt attcatctct tcttctttga tttttgctgt accgatatcc
     2041 agatttttat agatttgcaa tgaatcacca ctgatgatct ctcctccaaa tagttgtgcg
     2101 agttccacac ttagcgctgt tttaccgact gctgtcggac cgacgatcac taatactttt
     2161 tccatatttt acctactttt cgttgattgt ttccaccgaa gagctttctc cggaaaatca
     2221 gttattattc catctatttt gagttcttga catttggcca tttcttcttc atcgttaatc
     2281 gtccatgcac gaattgcttt tggaaaatca gctgcctgga ctggatgttc gaacaaccaa
     2341 gtattttttg gatgtactcc ttctatataa ggggtttctg ctgctagttg gatcttttta
     2401 tcagaagtac tcatcaataa gtccagctgt atctccggtt ccagtaatgc gattttttcc
     2461 aaagaatcca aatgaaagct gcaatatgca tgttgaaaag gccaatctct actttttaat
     2521 gtttcggcta atttttcttc gataccggga tattcatact gatcagtttt aatttcaatc
     2581 attagcatcc ctcggtaatt tttttggagg agcagtccta acacttctct caattcagga
     2641 actttagctg ctggaaattc tttgctgaat ttgccccctg cattcaattt tttgagttct
     2701 tccaatgttt tttctcgaat cagcccttta ccgttagtcg tccggtccac ttcttcgtcg
     2761 tgcataacga tcaaataacc atctttgctt agatggacat ctagttcgat accatctgca
     2821 cctactcgta cagcttctgc aaatgcagga agcgtatttt ctgggtgggt gccgctgctt
     2881 ccgcgatggg catagatttg taacatcttt cttcactccg ttcagttcta ttgtatcatt
     2941 aagaaaaaaa gaaagcatta ccacgctctc atgtgcaacg gcaatgcttc aaaatatcag
     3001 ctccaaacat catctttcga gaagaaacca atcggctggc gcgtcatagt cttcaactga
     3061 gctttttttt cattgatcat ttcagtcaga ctatccgtaa aattctctaa aatcacgcgt
     3121 ccgcctttat aagcatatcc accaatcttc attgtttcat ctggagataa aatcactttt
     3181 cgatgggaga tattcttaaa tagctcgtct aaaatataat ttggcagata aaaactattt
     3241 tgcgctgaaa atgattggag atacgcaaaa tcgtcaatcg tgatataaca atcatcaaat
     3301 gcatcaaagc tgacatattc ttccatcagc gttgtccgtt tttgcaagat ctcgttgatc
     3361 tttttattta attgattcag cgtctcgatt tctttgactt tcaattccaa ttcacgtcta
     3421 cgctgttctt tattttccaa gtgacgcatt tgcagtatca tgccgatata gacaactaca
     3481 gcacctgtca gcactccgat aaggccagta ataccaacta tcaatctgtt gttcctcctt
     3541 gttttgtttc tctgcttatc ataccataaa gcgggtttat gcgactaatt tgcctaagtt
     3601 ttttatttga ccttttttga ccaaaaatgt atactacatt ttaggtatta gaaactaaat
     3661 tatggtacac tatttctaaa cgaaatcaag gaggaactct attatgaatt taattcctac
     3721 agtcattgaa caatcttcac gaggtgaaag agcttacgat atctactcac gtttattgaa
     3781 agaccgtatc atcatgttaa gcggccaagt aacagatgat cttgctaatt cgatcatcgc
     3841 ccaattgctt ttcttagacg ctcaagattc tgaaaaagat atctatcttt atatcaattc
     3901 tcctggtggt tcagttacag caggtatggc tatttacgac acaatgaact ttgtcaaagc
     3961 agatgtccaa acaattgtta tgggtatggc tgcttcaatg ggaagcttct tgctgacagc
     4021 tggtacaaaa ggtaaacgat ttgctttacc aaatgctgag atcatgatcc atcaacctct
     4081 tggcggcgct caagggcaag caactgaaat cgaaattgcg gcacgccata ttttgcaaac
     4141 acgcgaacgc ttaaacaaaa ttttagctga acgcactgga caaccgcttg aagtgattga
     4201 aaaagataca gatcgtgaca actatatgac agctgaacaa gcaaaagcat atggattgat
     4261 cgatgaagtc atggaaaaca gttcttcatt aaattaatct gtttcaaagg aagctttttt
     4321 aagctctctt atctaaacca aataaaaaag gatggtcaac tcaaactgac catccttttt
     4381 tattttatgg atgctactgc ctatctttgc tccttgaatt ttccttgcgc atgcatccga
     4441 cctatctcga cataatgctg gatgttttct ttgttttttt gtatttcttc ttttgtcaaa
     4501 ggacggatca ctttagctgg gcgaccaaaa gccaagacat taggaggaat aacttttcct
     4561 tctgttacta atgatccagc accaatcaaa ctattctcac caataaccgc atgattcaat
     4621 atcgtggtcc ccatcccgat cagcgcccct ttttcgattg tacagccatg tagcatgcac
     4681 tgatgtccta ccgtcacatt ctccgcaata tctacaggtg catcatgatc gacatggatg
     4741 attgtaccat cttggatatt cgttctttgt cctattttta tccaattact gtccccgcgt
     4801 aaaacagctt gataccagat cgtcacatct tctgacaatg tcacatcacc aatcactgtg
     4861 gcatttgatg ctataaaccg ttccatattt atttttctcc tttcattttt tgtagaaact
     4921 aatcctattg aataaaggta tcatagcata tatttatttc ataagaaaaa aaacaggtaa
     4981 aacctgctca ggccagatgt ctatttacag tatctggtaa ctgacgtttt acctgttttt
     5041 attgtacatc tatattttct tctattactt tttttaaaat taattcgtac acttctattt
     5101 gtgctgccaa gcgttcgatt tgaaactcat tttcaaaaaa atcagctgaa tcaaaaggct
     5161 tcgtctgaca gacgtgcgtt aaacgatcaa cttctctttt ggcttcagta attttttcat
     5221 ttaaaaactg ttccatctcc gtgtcctcac ttttcttgat aactttactt taccattatc
     5281 aagaatagag aacaaaaaat aagttttaaa ctgttttatt cttatatgac ttattctgct
     5341 tcctcagcaa attggctgtt atacagtttt tcataaaatc caccttgtgc caataaactc
     5401 tcatgcgttc ctttttcaat gatctgacct tgatccatca ctagaatcag atctgcatca
     5461 cggattgtcg ataaacggtg agcgatgacg aaacttgtcc gtccctccat cacgcgatcc
     5521 attgcttttt gaatcaacgc ttctaaacgg gtgtctactg aacttgttgc ttcatctaaa
     5581 atcaagattt taggatcgga gaccactgct cgggcaatcg tcagcaactg tttttgtcct
     5641 aaagacacat tgtctccctc agaattgatg gccatatcat atccatctgg catcgttcgg
     5701 ataaaatgat ctacgtttgc tgttttcgca gcatcaacta cttcataatc tgtcgcatca
     5761 agctttccga agcggatatt atctgcaatc gttccttgat aaagccatgc atcttgcaag
     5821 accataccaa atagcgaacg cacatcactg cgactcattg atttggtgtc gattccatca
     5881 atccttattg ctcctttgtc tacgtcataa aaacgcatca atagattgat caatgtcgtc
     5941 ttgccggcac cggtcggacc gaccacagct acggtttgac cagctttcac ttcaaaattc
     6001 aaatcttgga ttaatggttt cttcggatca tatgcaaaat cgacatgatc gaatgtcaca
     6061 tctcctaaaa tcgtttctgg cagtgaagca tctactttgt tttctttttc ttctggctca
     6121 tctaaaatct cgaatacacg tttcgttgcc gctgatgcac tttgcaaaac tgaagacaat
     6181 tgtgtcacgt tccccattgg ctgactgatt tgccaaatat attggatgaa tgcttgtagt
     6241 tgaccgacaa cgatcgcccc agtaataaca taaaagcttc ctagaacagc cataccaata
     6301 taagtagcat aggcagttaa ttgaactaga ggcatcatca agccagaaat gaatgctgca
     6361 cgaaacccaa agtatttcaa gctgtgatta acttttttga atccttccaa tgtttctttt
     6421 tcgcgtccat atactttcaa tacactaaaa cctgtcatat tttcttggac gtagccgttt
     6481 agctcaccaa gagaattctg catgctttga aaatatttct gcgaagcatt gatcactcgt
     6541 tttgagatcc agatcgatgt tggaatcatg atcatagaga tcagcgccat catcggctgg
     6601 atataaaaca tcattgctgc agccaacgta attcccaata tcgcatttac gataccaata
     6661 aaagcttgtt gtaaagcacc gctgactgca tctacatcat tcgtcacacg agacaaaata
     6721 tttccttgct ggtttttatc gaaatatgaa accggaagac gattgatttt ttcttctatg
     6781 tctcttctca aatcatgcat ggcactttga acgacatttg tcatcaaaac accagaaagt
     6841 aattgcgaaa tacaataccc tattccaaca actgtgatcc aaatgagaca tttgaatata
     6901 taagaaaaat tgattgattc gccattggca atattacggc tgatttctgt agtaggaagt
     6961 cctgtaacat atggcaaagc cacgttgaaa gccactgtca gaactgtaaa taggatcact
     7021 aaaagaaaag tctcacgata cggtctgata tatcgagcta aacgttttaa ggaagaaaag
     7081 gtttttttca tgctaattcc tcctttgata attgagaagc ggcaatatca taataaatcg
     7141 ggcatttttc caacaactcc cgatgcgtcc ccataccgac gacttctcct tcatttaata
     7201 cgatgatctt atctgcatgc atgatcgttc ccacacgttg tgctacgatc aatacagtag
     7261 attctgtcgt ttcttttttc agacgagcac gtaacttcgc atccgtttga taatctaatg
     7321 cagaaaagct gtcatcaaaa atatagattt ccggacggcg tatgatggct ctggctattg
     7381 ccaatcgttg tttttgtcca cctgaaaaat tggaaccacc ttctgataaa tgttcattaa
     7441 atccatctgg ttttcgagaa atgaattctg aagcttgagc aatctcagct gcacgttcca
     7501 tttcttcttg cgtagcatct tctttcccgt atcgcaaatt atctgcaatc gttccagtaa
     7561 atagcagcgc tttttgcgga ataaacccaa tcttatctcg caacttgctt agccgataat
     7621 ctctcacgtc tactccatcg atcaagatct ttccttcact cacatcataa aagcgaggga
     7681 tcaattggat caacgtagat tttccgctac cggtacttcc aataaaagca acggtctctc
     7741 ctggagaggc tgtaaaactg acatttcgta tcactggctc ttgcgaatgg cctggataag
     7801 caaatgttac attttgaaat tcgatatacc ctttagtttt tgtatcagat ataccatttg
     7861 gatcttctac aatcgaaggt ttggcatcta gggcttcttg gatacgtcca gctgaaacag
     7921 ctgctcttgg atacatcatg aacacactag caaataacat gaatgagaac aaggcatgga
     7981 atatatactc gataaacgca atcaaatctc ctacacgaag ctcgcctgca tcgatttgca
     8041 ctgccccgct ccagatgatc aataccatta cgatattaaa taagaaataa aaacccggtt
     8101 gtgcaacagc catcaaacgg aataagcttt ttgaactaga agcataattc tcattgacta
     8161 cagcaaaacg tttttcttca aacttttcat tgacgaatgc acgaatcaca cgcaatcctg
     8221 aaagattttc ccgcaagata ctgttgattt tatctaagtt tttttgttgc tttttagata
     8281 acggttcaga caccttagca atcgctacga ctgccaataa tagtaaaggt aatgcaccta
     8341 ctacatacaa actgagtgtc acacttgtac gcatgaccat gtacaaactg acaatgaaca
     8401 tcattggtgc cataaaaccg atacgtaaaa tattttgcag gaacaacatg atctgataag
     8461 catcgtttgt tgtacgagtg atcaatgaag atacgcctag attttcgtat tcctgatgcg
     8521 aataactttg gatcttttcg aacaaatcat ctcgaatatc tgctacgata ttagtcgtaa
     8581 tcctcgaacc aaaataacct aacaagatat tcaaaataat accgattaca gtaataacga
     8641 tcataaatat cccttgttgc ttcacataat caaaatcatc attgcggatt cctacatcga
     8701 tcatacgtgc taaaatcgta ggaagtccta attcaatcaa aataaaacca aatacgcata
     8761 tgaaattgaa aaacaagaat ttcttataac gcatggtata atgccacatt agtttcactt
     8821 tctcatctcc ttttaatctt ttgttacatt ttcattacta aaaatgatag cacaacgagc
     8881 ctattaatag aacacttctc tcataaaaaa gtcattatga ttatagcggg atgataaaca
     8941 ttgatttttt ctttcaatct agttgatttc tatacttatt tgctgtttta tgctagaata
     9001 acaattaatt gttgtatatt ttttatacag aaggtacggt tgaaaaaata tccgaacaga
     9061 taagcaaaaa cgaggcttgt ctctttgact cgaatccagt attctagtca aagcatatga
     9121 aaaaccaggt ggcttgtccc ctcgttttgt gttgtctaaa aaaatcaact agctaaatca
     9181 ctgctttacg cagataaagg agacaaaaca atgatctata aagtttatta tcaagaaaca
     9241 aaagtacgca accctaaacg ggaagaaaca aaatctcttt acattgaggc aaaaagcgat
     9301 gtagacgctc gccaacgtgt ggaagaaaat accccataca atattgaatt cgttcaactt
     9361 ctagaaggaa gtcatctaga gtacgaaaaa gaaaatgctg atttctcatt agtggagttt
     9421 taatatgaaa ctttccatca aaaataacga gacaggcgtc tttgctattg gtggattagg
     9481 tgaaatcggg aaaaatacgt atggggtaca attccaagat gaaatcattt tgattgatgc
     9541 cgggatcaaa ttcccggaag acgatttact tggaattgac tatgtcattc ccgactacag
     9601 ctacatcata caaaacttac acaaagtaaa agccttggtg attactcacg ggcacgaaga
     9661 ccacatcggc ggaataccct acttgctgaa acaagcaaat attcctatct atgctggtcc
     9721 tctcgcatta gcgttgatca caaacaaatt agatgagcat ggcttgcttc gagatgcaga
     9781 gctccatgag atcaatgaag atacgatcat ccgttttaga aagacttctg tcagcttttt
     9841 cagaacgacc catagtatcc cagatgcatt agggatcgtg gtgaaaacac cttctggaaa
     9901 tatcgtagcg actggagact tcaaattcga tttcactcct gttggcgaac cagcaaactt
     9961 gcataaaatg gctcgtatcg gggaagaagg agtcttgtgc cttttatctg atagtacaaa
    10021 tgctgagatt ccaacattta ctaaatcaga aaaaacgatt gggcattcta ttttgaagat
    10081 cttttcaaaa atcgaaggac gtatcatttt tgccagcttt gcttctaaca tcttccgttt
    10141 gcagcaagca gcagatgccg ctgtggcaac tggtcgtaaa atcgctgttt tcggacgaag
    10201 catggaaaat gccatcgtca acggtcaacg tttaggatat atcaaagttc cagacggcac
    10261 atttgtagat gcacacgaaa tcaatcgctt gcctgccaat gaaacgatga tcctatgtac
    10321 aggttctcaa ggagaagcga tggccgcgtt gagccgcatc gcgaatggta ctcaccgtca
    10381 aatccaaatc cagccagacg atacagtgat cttttcaagt tcacctattc ctggtaatac
    10441 aacaagtgtc aatcatttga tcaatttgct gtctgaagct ggtgcagaag tgattcacgg
    10501 aaaaatcaat aacatccata cttccggtca cggtggacaa gaagaacaaa aattaatgct
    10561 tcgtttgatg aagccaaaat attttattcc tgtccacgga gaattccgca tgttgaaagt
    10621 acatgcttct ctagcacaag atgtaggtgt tcctgcagaa aactgcttca tcatggaaaa
    10681 tggcgatgtt ttagcgttaa ctagcgattc tgcgcgtcct gctgggcatt ttgtggctgg
    10741 agatgtgtat atcgatggta gcgggatcgg tgatattggt aatgtcgttt tacgtgaccg
    10801 tcacctgctt tcagaagaag gtcttgtatt agctgtcgcg acagtggata tcaagaaaaa
    10861 agaaatcttg gctggtccag atattctctc tcgtggattt gtctacatgc gagaatcagg
    10921 agaaatgatc aatgaagctc agcgaatctt attccacgct ttaagagaat gtttgaatgg
    10981 acctgactgt tcagaatata aattgaaaga agcgatgact ggcgcgcttc agcctttctt
    11041 attcgaacgt acagaaagac atccaatgat tttgccaatg gtaatgacac cagatgaaga
    11101 ataaataaga ggttatgaaa tcagcacctt gtcctgaata taagagaaaa caaatgaaaa
    11161 acatgagaag ccatttttgt ttgttcttat tccttgatac aaggtggctc tttcatagcc
    11221 tcttaaattt atttactttt tcatacaaaa aaatacctgc cacaagagaa attgtctctt
    11281 gtggcaggta tttttgttac tcgctttgtt ctctcgtaaa aattgatgtc taccatcaat
    11341 ttgatttaca gataaaatta agcttttaca acgtttgcag cttgtgggcc gcggtctgat
    11401 tcttcaacgt cgaaagtcac tgcttgacct tcttctaatg ttttgaatcc gtcaccttgg
    11461 atagctgaga agtgaacgaa tacgtcgctt ccatcttcac gtgagataaa tccaaatcct
    11521 ttttcagcgt tgaaccattt tactgttccg ttttccataa taatatcctc ctcgtgccaa
    11581 aagcactaat ttaaattgta acattgctcg tgtacgatag aggtgcaaca gatgtttcaa
    11641 cttctattcc caaacaaaat tacaatataa ttgtacactt gcttcatttt ttttgcaaga
    11701 aagcaactta cttctcttcc atcattcctt cttctctcaa actaaaatat cgtttgcgtc
    11761 cgatgatcaa gtggtccagt acagtgatcc ccatcatttc tccacacttt tgtatccttt
    11821 tggtaaaatt catatcctgc tgcgatggaa tttcatatta tcaagagtaa attacaaaag
    11881 aatcatttaa atcaatactt tcactttgaa tacatgtatt tgaagtggaa ggtacttatt
    11941 tcaaaaatag taaaacctgt atcttaaatt acttaatagt aacataagat acaggctgat
    12001 tttttattca ttgttattta tactaaagca cccgatagct ctgaaaacaa tcacaaattc
    12061 aacttttcaa agccacagct ttaagttatt ttgtccagac aacccccatt gcccaaccca
    12121 ttttatggaa ttggcatcca ggcaacaact tttcatataa atcgtaataa ttttgttcag
    12181 ataggtactt atctgaagct aaatgctcaa gccatgattt agatgtgttg tgattataaa
    12241 ttctaatcgc attttttatt ccaaatttga aacaatgcgg taaaaactct tggattgccc
    12301 ctaatttata aacataggta ggcggtgtct cgacttcaga tacattatca agaataacta
    12361 ttcttccttc ctcattcaac agttccttca tttgctgtat tacgctggct atatcatcca
    12421 aatgatgaaa ggttgttcgg cttacaataa aatcaaactt ctcattaaaa ttaagttgtt
    12481 ctgcattcat attcagatag accgtatttg ttagttgacg tttagatttg gcaagatcga
    12541 gcatttgatt agaaatatca atccctacca cttcatcata ataacttgct aatttctcca
    12601 ctaacaaacc cgagccacat ccgatatcta atgctctgcc tttctttgga gacatattag
    12661 acacaaagaa tgaataatca ttcaaaagct cattcacgaa atcgtaatct tctgcaacct
    12721 tatcaaactg tgattctatt gtattcaaaa agatccccca ttcctacttt atcgacattc
    12781 tttcattact taccacttta gatgtttttt cgttggggat aaaacttccc tttagacaat
    12841 tttatccaaa gacaatacaa cagtgcaact ttattaaagt cactgtcctt tatcgcagcc
    12901 tttacttttt agtaaagaca gtggcttctc ttatcaagtt tcaaaacata ttattttgaa
    12961 gaaaacgtcc atctgaagtg tcaagtgcaa aattacatat aaaggtttat tctaaaatga
    13021 aaagatgata caatcatatt cagttacata aggaggtttc aattatgtgc accagtatcg
    13081 cagtagtaga aattacttta tctcattcat aatgaaaaaa atggaaagga gataaaagta
    13141 tgggtacttt ttctatattt gttattaata aagttcgtta tcaaccaaat caaaattaat
    13201 tggttataat gaacgcttaa tgtcagttca ttataaccag taaggagaag gttataatga
    13261 accagaaaaa ccctaaagac acgcaaaatt ttattacttc taaaaagcat gtaaaagaaa
    13321 tattgaatca cacgaatatc agtaaacaag acaacgtaat agaaatcgga tcaggaaaag
    13381 gacattttac caaagagcta gtcaaaatga gtcgatcagt tactgctata gaaattgatg
    13441 gaggcttatg tcaagtgact aaagaagcgg taaacccctc tgagaatata aaagtgattc
    13501 aaacggatat tctaaaattt tccttcccaa aacatataaa ctataagata tatggtaata
    13561 ttccttataa catcagtacg gatattgtca aaagaattac ctttgaaagt caggctaaat
    13621 atagctatct tatcgttgag aagggatttg cgaaaagatt gcaaaatctg caacgagctt
    13681 tgggtttact attaatggtg gagatggata taaaaatgct caaaaaagta ccaccactat
    13741 attttcatcc taagccaagt gtagactctg tattgattgt tcttgaacga catcaaccat
    13801 tgatttcaaa gaaggactac aaaaagtatc gatcttttgt ttataagtgg gtaaaccgtg
    13861 aatatcgtgt tcttttcact aaaaaccaat tccgacaggc tttgaagca
//
LOCUS       DAYYCY010000064        13877 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000064 DAYYCY010000000
VERSION     DAYYCY010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13877
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.064"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            60..2225
                     /gene="acm"
                     /locus_tag="ACHAZB_001504"
     CDS             60..2225
                     /gene="acm"
                     /locus_tag="ACHAZB_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HFM8614183.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(2497..2982)
                     /locus_tag="ACHAZB_001505"
     CDS             complement(2497..2982)
                     /locus_tag="ACHAZB_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HFM8614184.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    3072..3417
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3462..4103
                     /locus_tag="ACHAZB_001506"
     CDS             3462..4103
                     /locus_tag="ACHAZB_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFM8614185.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            4122..4757
                     /locus_tag="ACHAZB_001507"
     CDS             4122..4757
                     /locus_tag="ACHAZB_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM8614186.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            4775..5590
                     /locus_tag="ACHAZB_001508"
     CDS             4775..5590
                     /locus_tag="ACHAZB_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8614187.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            5909..7345
                     /locus_tag="ACHAZB_001509"
     CDS             5909..7345
                     /locus_tag="ACHAZB_001509"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8614188.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            7534..9981
                     /locus_tag="ACHAZB_001510"
     CDS             7534..9981
                     /locus_tag="ACHAZB_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFM8614189.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            10211..11278
                     /gene="rlmN"
                     /locus_tag="ACHAZB_001511"
     CDS             10211..11278
                     /gene="rlmN"
                     /locus_tag="ACHAZB_001511"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFM8614190.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            11268..11474
                     /locus_tag="ACHAZB_001512"
     CDS             11268..11474
                     /locus_tag="ACHAZB_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8614191.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(11471..12574)
                     /locus_tag="ACHAZB_001513"
     CDS             complement(11471..12574)
                     /locus_tag="ACHAZB_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFM8614192.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            13127..13675
                     /gene="aac(6')"
                     /locus_tag="ACHAZB_001514"
     CDS             13127..13675
                     /gene="aac(6')"
                     /locus_tag="ACHAZB_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFM8614193.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         4749 a         2306 c         2938 g         3884 t
ORIGIN      
        1 atttttgaga gatgatatag taggttttat ttttagttaa aataggggag atagaaaaaa
       61 tgaaaaagtg tagaaatatt attttatcaa tgttatttat tattactaat ataacatcac
      121 tgattcctgt tcatgtctat gcggatgcag gcagagatat cagcagtaat gtcacttcgt
      181 tgaccgtgga cccaacgaat attacagatg gaggaaacat aaaagtaaaa ttctcgtttg
      241 acgaaaaaaa acaaaatatc caaccaggtg attatctttg gatcaattgg ccaagtgaag
      301 gaaatattcg cggtgaagga ttccaaaaag aaatcccatt aatgattgaa aataaaaatg
      361 ttggaacttt aacggtcaga aaagattctg cgcaggttgt gtttaatgaa aatattaaaa
      421 acctagactc agtagaaggt tgggggcaat ttgagataca ggccagaaac gtaaccgata
      481 ccggtgaaga aaatacggga ccattcaccg tgacgagcgg tgataaaaca gctactgtaa
      541 atgtgacaaa gccagcttct ggttcttctt ctagcgtttt ttattataaa acaggagaca
      601 tgctacctga agatactaag cacattcgat ggtttttgaa tattaacaat aacggcactt
      661 atgttgaaca accagttaaa atcagtgatg aaatccaaag tggacaaaga ttagatccaa
      721 gtacgtttga aattaatcaa atacacttag gtgaacagaa ggtttataga ggagaagagg
      781 gaatccaaca atttttacaa gatttcccca gcgctacatt taatttttca gtaactgata
      841 attacattga aataactata ccaaaaaact ttgtcaatct taggaaaata atggtgagtt
      901 ataagacgat catagaaaac ccagaacaaa taaattttga gaatcattcg gaagcttggt
      961 ttaaagaatt taataaacct gctgttgatg gagaaagttt caatcataca gttaaaaata
     1021 tttcagcttc tggtggagtc aacggtacag ttagaggcga attgaagatt ttcaaatata
     1081 tcaatgacac agagattggt atcccaaatg tgactttcga attaagacga gcagatgaac
     1141 agcctattca aggacagtct agtatcctgt tgacttcgaa tgaacaggga gaagctagta
     1201 tcaaaggcct gcaagtagga gactatgttg taaaagaaaa agaagcaccc aattggattg
     1261 attttgatcc actaagttca aatgaactca aattctccat aaacgaaaat gatacagagg
     1321 gagtcagttt acctatttat aacaagaaaa aggttacgaa catcacagca actaaaattt
     1381 ggaacggtgg aaccactcca agaccgagta tttattttaa attattcaga gcaagtacaa
     1441 acaattggga accagttcct gatgcagaaa caaaacgatt agacaatgga ataacttcag
     1501 tgacttggga agatatccaa cagtatgatg atagtggaaa cgaatatacg tttaaagtgc
     1561 aagaagtaga tcaaaacgga aatgattatg tcccttcagg ttatcaaaaa attgaaaacg
     1621 ggctagtcgt tacaaatgag aataaagaag tcatttcagt ctcaggacaa aaaacatggg
     1681 aagacaatga gaaccaagac ggcaaacgac cgacgacgat cacagtaaat ctattagccg
     1741 acggtcaacc gatccaacac aaagaagtaa gtgaaaaaga tgattggcgc tatcgtttca
     1801 caaatctgcc aaaatacaga gacgggcaag aaatcatcta tactgtaacg gaagacaacg
     1861 tgcccgaata cagtacgacg attgacgggt ataacatcaa aaacagttat acaccggaaa
     1921 aaacaaatat atctatacca ggtagccata taacaccgtg gaaaaatttt ccagagaaga
     1981 cagagagaga gaaaattaga aatctattct ctcaaaaatt gcaaatattt gctcatacag
     2041 gagaatccag cgaacaaaga agcaattata ttagtaagag agctgtacct aaacagataa
     2101 caaataaatc aaaaagcatt cttcctaaaa caagtagtaa aaaatctagt tttgctgtaa
     2161 tcattggttt attgatagta actatctgtg ctgggatatt cttacaaaaa tataaaaaag
     2221 tttagttaga atataatatt ttttgttaaa tatgactgaa atcaaaagaa taaaattccg
     2281 atttataatc gaaaaaagaa gcatttttat atatgcttct tttttcgtag aatcaataaa
     2341 tatggatttg gctaactatg gaagaatact cgtattagtt ttcagcgttc atactagttg
     2401 ttacattgtt tgattggcca aaagaatttc tgatatattt tgtgctaaat aaagaaaaag
     2461 acgcttgaat tgggaaataa gaaaactact ctatcattac aaattttcat aaactggcag
     2521 atgttctaag taaacaagta tcttttcaaa gtcggaaaaa tccataatca acgtagtggt
     2581 attgatattt ggatgaaagc ctacggtatc ttgacgatca atagcagcat cgatcacgac
     2641 ttggattttt ttctctttgt cgtgcatcaa tgctaatgga gtcactgtac ctgctggaac
     2701 gcctaggagg tcaggaagct gttcttcaga aacaaaagat aagcgctttt cttttaattg
     2761 ttcagctagt tgttttaaat cggcgtgctt ttcatcaggc aagatgacaa gatagatttg
     2821 ttttcctttt cttgatttca ataatagatt ttttacttgc ggaccaggta gggaaaaagg
     2881 gacatttttg actgacgtaa tcgcaggatg atcgactttt tgaaaagaga tacccagttt
     2941 ttttagtagt tcataagctt cttgttcggt tgcacgactc atcgcgcatc accctccttt
     3001 ttttattagt gtatcataaa aaagagttga caaatgaaaa ataatcatgt aaattacacg
     3061 taaagttaaa acaaatgaca tcgaaaagaa gagtagccgt taggtgtttt tagagagtct
     3121 ctggttggtg gaaagaggca gcagttaacc gtgaaacaca tcttggagtc gattttctga
     3181 agcgtatagt aggaaaatcc ggggagcccg ttacagctgc ggtcgttgct agatgaccag
     3241 ataaggctgg cttttccgtg aggagtcggt aaaaaaaggt ggaaccacgt gattaacgtc
     3301 ctttgatctt cggatcaaag gactttttgt tttcatctga acggcaacga ctgtgtgagg
     3361 ttttttctgc aaggaaaaaa taaacagagg tggaaccacg tataatacgt cctcttggca
     3421 tttgccaaga ggattttttt attttaaagg aggaaaagaa gatggagtat ttgattgaag
     3481 ttatgccgca attattagta ggagcactca caacattgaa attattttta tttaccttga
     3541 ttggctcatt accattaggg attgttttag cattaggatt gtctttgcat tttaagccgc
     3601 tacgctactt gcttcaaaca tatgtatggg ttatgcgggg aacaccgctg ttgttgcaac
     3661 tgatttttat tttttatggg ttgccgacga ttgggatcgt ttttgatcgt ttcgaagcag
     3721 cactcattgc ctttatctta aactatgcag cttattttgc agagatcttt cgtggaggga
     3781 tacaatcgat tcctcaaggt caatatgaag cagcgcaagt tttgcaatta agtccctttc
     3841 aaacggtgac gaaaatcgtc atcccacaag tgacaaagat tgttttacct tctgtaggga
     3901 atgaagttat caacttggtc aaagattctt ccttgatcta tatattaggt ttaggtgacg
     3961 taatgcgtgc tgggaaaatc gcgatggaac gcgacgcgac tttactgcct ttgctaggcg
     4021 tggcaatcat ctatcttaca ttgactggta ttctaacaat cataatgaaa caagttgaag
     4081 gaaaattaaa ttattaccgc taactgatag gaggaagaaa tatgattgaa ttgaagaaag
     4141 tgaacaaagc atttggtgaa aaaaaaataa tcaaagattt gaatctaatc attccagatg
     4201 ggaaaacatt agctatcgta ggtccttccg gtggcggtaa aaccacgctt ttacgtatct
     4261 tggctggctt agaaagttct gacagcggag aattttattt agacggtgta ccgttcaacc
     4321 cattcgagct taaaatgaaa gaacaagttg taggagtcgt attccaagac ttccagctat
     4381 ttccacatct gaatattttt gaaaacatta ccttagcacc aaaaatggta ttgaaacaga
     4441 caaaagaaga atacaccaaa aaagcagaag aattagcggc ttcattaggg attagtgatt
     4501 tattgaatat ctatccttat cagctttctg gcggacaaaa acagcgggta gcgattgcca
     4561 gagcattggc tatgaatcca cgggtattgg cttatgatga accaacaagt gctttagatc
     4621 ctgaattacg ccagcaagtc gagcgtttga tcctatcatt gaaagaagat ggggtaaccc
     4681 aaattattgt cacccatgat ttagtatttg cagaaaatgt cggtgatcaa ttactaaaag
     4741 tttccccaat tcattaagaa aggagaagat agagatgaaa aaaaccagtt ttctattcgc
     4801 tttgatcgca ggtcttcttc tttttgcagg ctgttcgaac aaaaaaactt cagcagataa
     4861 ttgggcgact atcaacgaag agaaaaaaat cgttataggt ttagacgata catttgttcc
     4921 tatgggattt agagataaag acgggaagct cacaggattt gatattgatt tagccaaagc
     4981 agtctttgaa caatatggga ttacggtaga ctttcagccc attgactggt caatgaagga
     5041 atttgaactg aacaatggaa cgatcgattt gatatggaac ggctattcca aaacgccggc
     5101 aagagaaaag aaagtgcaat ttacaaaacc ttatatggaa aacgatcaag tgctgatcac
     5161 acctaaagaa tcaggaatca caaactttgg acaaatgaat aataagagat taggtgcgca
     5221 aaatgggtca tctggttatg atgtatttac gaaacaacca gaagtattaa aagagatagt
     5281 caaaaaccaa gatgctgttt tgtatgatag ttttaatgaa gcattgatcg atctgaaaag
     5341 cagacgggtc gatggtctgc tgatggataa agtatacgca gactattatt tgaaacaaag
     5401 aaacgaacta gaagacttca acataacaaa aggttcttat gagagtgagg attttgcagt
     5461 tggtgcacgc aagtcagata gtacattagt gaaaaaaatc aatgaagccc tagatcaact
     5521 agagaaaaca ggtgaattcc aaaaaatatc cgataaatgg tttggcgaag atgttacgcc
     5581 gagaaattaa caaatagggg actgtgacaa aagacgtgtc acagtccctt tttccgaata
     5641 aacagtgttc tcctgccaac tccttgtgac tcggagctga atggcaggct cacaacctct
     5701 tggtaaacgg tgttcaaaag ccagtttttc ggtattaagc caaatttatg caaaaatgta
     5761 gaaagccatt ttcacaaaat ttttcttact actcgaaact aagcgacttt ttcacaacct
     5821 cctttacata attttatttt gttgtggaag gggtttcgtg ttattctaaa agaggaacga
     5881 ttgaaggtta taggaaagga agagatgaat ggaacacaaa gaacttacct catttccaaa
     5941 agattttctg tggggctctg cttctgcagc gtatcaagta gaaggagctt ggcaagaaga
     6001 tgggaaagga gaatcagtct gggatcgatt tgtacggata cctggaaaga catttaaagg
     6061 aacgaacggt gatttggcag tagaccacta tcatcgttat aaagaagata ttgctttaat
     6121 gaaagaacaa ggtctgaaag cttaccgctt ctctgtagct tggacacgga tttttcctaa
     6181 tggacgagga gaagtcaatc aagctgggct cgctttttat gagagattga tcgacgaatt
     6241 gattgaaaac gatattgaac ctatgttgac tttatatcat tgggatcttc cacaagcatt
     6301 gcaagatgaa tataatggtt gggaatcacg tcagatcatt gaagatttca ctcattatgc
     6361 agaaacatta tttgaggcat tccgcgggaa agtccattac tggattagtt tgaatgaaca
     6421 aaatatcttc acttcattag gttatttgtt agcagcacat ccgccaggtg tgacagaccc
     6481 gaaacgaatg tatgaagtta accatattgc taatttagca aatgcatctg tcatcaataa
     6541 atttcacgag ttagaaatgc ctggaaaaat cggaccaagc tttgcgtata cgccaaatta
     6601 tccaattgac agtaatcctg aaaatgttct ggctgctgaa aatgcagaag acctgatggc
     6661 caattattgg atggatgtct atatgtgggg aaaatatccg attgctgcca tgaggttctt
     6721 agaagaaaaa ggatgggcac caacgattga agctggcgat gcagaattgc tagaatctgc
     6781 taaaccagat tttcttggga tcaattacta tcaaacggcg acgaatgcat tcaatccatt
     6841 aaacggagtt ggtgctggaa aaatgaatac gacaggaaaa aaaggttcat cagaagaaac
     6901 tggagttcct ggaatgtata aaaaagttga gaatccattt gtcgaacgaa caaattggga
     6961 ctgggagatt gacccagaag gattaaggat cggactgcgt cgtatcacaa gccgttaccg
     7021 catgccggta ctgattacgg aaaatggctt aggagagtat gacaagttga cagaagataa
     7081 acaaatccat gacgactatc gaatcaatta cttacaaagc cacgtcaaag ccatcaaaga
     7141 agcaatttcg gatggagcag aagtcctagg ctattgcacc tggtcttata cagatttgct
     7201 tagctggctg aacggttatc aaaagcgata tggatttgta tatgttgatc aagacgaaac
     7261 acaagaagga tctttggaac gatataagaa agacagttat tactggtacc aaaaactgat
     7321 tgaagaaaat ggagagaacc tttagttcac atacttaaaa caagaaggta cggctggata
     7381 tggccgtgcc ttttcttgat aaaatcattt agaaattaag aaaaactgta aaataagctg
     7441 aaaaatgaaa gaaagcgtca gttctttcaa acaaaacttt agttccctta cttttttttg
     7501 tataatgaaa cttgatgaaa tgagggataa taaatggcac aaaagaaacg gcctactaaa
     7561 aaaaggaaaa agacgaaaaa aacgaaaaag caaaaacagc agcaggaaca aatgctgacg
     7621 attggtgttg ggtttgcctt gattttattt gctgtcttcg gctttttaaa attaggtttt
     7681 ttaggaatat tgattgcaaa cggctttcgg atcattgcag gaaatactta ccaaatcctc
     7741 tgtcttttat tagccgtatt aggattttgg attgtcatta agaatacaga gttttcaatt
     7801 ggtaaaaacc gacgctggtt tggcggcata ctcttttatt tcggtatctt attattgtta
     7861 catgcccatt tatttggcaa actccatact ggggaaccaa atatcatggg gacgacatgg
     7921 gatttgttag ccagtgatat aaaacaaagt caagtagaca ataacgttgg tggtgggatg
     7981 attggagcaa tcctttatca ttttacttac ttcttgattg cacagcctgg aagttattta
     8041 gcagcagtgc tattactggc aggaggagca ttcctgttca gtaatctgga aggctatcag
     8101 ctgttaaatg gtatccaatc aattggagaa cgagtacagg aattattgga aggtgatcct
     8161 gccaagcaag ctcgaaagca agcggcaaag gaagagagaa tgaaacagcg cgcagaagcg
     8221 aaagaagcaa ggcgcttagc tgcaaaagaa gctgcagaaa aagaagctgc agaatatgag
     8281 aagaaaaaag cggtgagcca gcaaagggaa aatactgctg atgaatggca ggaaccagaa
     8341 aatacagagc cggagcagct aagttttgtt ccaatcgaca gttttcaaga aaatatccat
     8401 cctgcgaatc tagagaaacc agtaccagat acaccaaaac agaccaatac agcagaaggt
     8461 ttttcagatg agttgccaga ggatgacggt acctcattag aatttgagat tgaagcagaa
     8521 caggaaaatc aagactatga attaccgaca gttgatttgc ttgattctat accaactgtg
     8581 gatcaaagtg atgagtataa aaaaattgaa aaaaatatcg gcgtattaga acaaactttc
     8641 caaagtttcg gtgtcgatgc caaggtcgtc aaagccagct taggaccatc cgtcacaaaa
     8701 ttcgaagtcc agccggctgt aggggtaaaa gtaagtaaaa ttgtgaactt aacagatgat
     8761 atcgcgcttg ctttagcggc gaaagatgtc cggatggaag caccgattcc aggtaaatca
     8821 ctgattggta ttgaagtacc aaatggaaag atcagtatgg tttcctttag agaaatcatc
     8881 gaagcacaac caaaccaccc agataaatta ctagaagtac ctttaggacg tgatgtttct
     8941 ggacgggtac aaacggctga tctttcgaag atgcctcact tactcgtggc tggttcgact
     9001 ggtagtggga agtcagtagc aattaatggg attatcacaa gtatattaat gcgtgctaag
     9061 ccccatgaag taaaactgat gatgatcgat ccgaaaatgg ttgaattgaa tatgtacaac
     9121 ggcatacctc atctgttgac gccggtcgtg acgaacccta gaaaagcagc gcaagcctta
     9181 caaaaagtag tacaagaaat ggaagaacga tatgaaaaat tcgcggcaac cggagtaaga
     9241 aatatttctg gttacaatga atttgttcaa cagaaaaact tggaaaacgg aacgaaacat
     9301 ccaaccttac cattcatcgt agtgatcgta gatgaattag ctgatttgat gatggtagcc
     9361 agcaatgaag ttgaagatgc gattattcgt ttagcgcaaa tggctcgggc agcggggatt
     9421 catatgatcc tagctactca acgtccaagt gtggatgtta tcacaggaat catcaaagca
     9481 aatgtacctt ccagaatggc atttgcggta tcaagcggta cagattctcg gacgatcatt
     9541 gattctaatg gggcagaaaa gctcttaggg cgaggagata tgctattctt gccaatgggt
     9601 gagaataaac cgatccgtgt gcaaggagca ttcatttctg accacgaagt agaacgggtc
     9661 gttcaatttg ttacagatca gcaagaagct cattatgaag agaaaatgat gccgacagat
     9721 gaagttgaaa cgacaggtgc tccagaacag ccacaagatg agctgtttga agaagcaaaa
     9781 gcacttgtcg ttgaaatgca aacggcaagt atttcccttc tccaaagaag atttcggatc
     9841 ggttacaacc gagcagcacg tttagtagat gaactggaag ctcatggagt tgttggcccg
     9901 tcagaaggaa gcaagcctcg gaaagtattc attgagcagg aagaaacatc agaaacgttg
     9961 ccggacgata atgacatgta aaacttgtgt gatgaagggt tgaaaaacga ataaccaata
    10021 aaaagacaag gcatgatgat accaaaagaa aaaatggttt ttttccatct tttctaatac
    10081 ggtatgatgt cttgtctttt tagtttttgt gagattaaag ttctattcga tttatttgtg
    10141 aagtattgca ttaaggaaat gaatcatgta tagtatgtag cgaaactttg agaaaaagag
    10201 ggaaaatact ttggaaaaac agtccattta tggcttgacg aatgaagaat taatcaattg
    10261 gtttatagaa aacggcgaga aaaaattccg agctgcccaa gtttgggaat ggttgtatca
    10321 aaaacgtgta agtaatttca cagaaatgac gaatctatcg aaacaattga tcgaaaaatt
    10381 atcggagcat tttatcatca atcctctaaa acagatggtc gtccaagaag cttcggatgg
    10441 tacagtaaaa tatttgtttg aactccctga taaaaacatg attgagactg tactgatgag
    10501 acaagaatat ggcttgtcag tttgtgtgac gacacaagta ggatgtaata tcggctgtac
    10561 gttttgtgca agcgggctgc tcaaaaagaa ccgtgatctg actgctggag aaattgttgc
    10621 acagatcatg atggtccaac attattttga tgagcgcaat cttggcgaac gcgtttcaca
    10681 tgtcgttgta atggggatcg gtgagccatt tgataattac gacaatgtaa tggacttttt
    10741 gcatatcatc aatgatgcca aaggattggc aattggtgct cggcacatta cagtttccac
    10801 tagtggatta gcacacaaaa tcaaggaatt cgctaataac ggcttgcaag tcaatctggc
    10861 tatttccttg cacgcgccga ataatgaggt gcgaacatcg atcatgcgga tcaaccgaag
    10921 tttcccaatt gagaaattga tggaagctgt ggatgagtat ttagagaaaa caaaccgtcg
    10981 aatcacgttt gaatatatca tgttgaatca agtaaatgat agaccagagc atgcgcaaca
    11041 attagcagat ctattaaaag ataagaaaaa attagcatat gtcaacttga ttccatataa
    11101 tccagtcagt gaacatgatc aatattctcg cagtccaaaa gctgatgtat tgagatttta
    11161 tgatgtattg aagaaaaacg gcgtaaactg tgtgatacgt aaagaacatg gtacagatat
    11221 tgatgcagct tgcggacaat tgcgaagcaa gcaaatgaaa aaaacagttg ccaaataatc
    11281 gtatcctact cgttttttca attaccataa aaggagcagt tttcttaatt aaggaactgt
    11341 tcaacttaaa taagagaaaa gattctatag cgaaagctat aaagcttttt ttgacaagaa
    11401 aaaccctcat caagcaaaga aatgtcctca atttgatgac agcctgcttg atgggggttt
    11461 tagcaattat ttaactgatt ttcttatatc cttgctggct ttcttgtgta gcatgataag
    11521 tttctacgtc aagaatggcc tgtttaaatt tttctcgggt gatttcataa ggcacatgtt
    11581 taatatcatt tgttgccatg gctttatcta agattgcttc taaatcggtc gtgacattga
    11641 gattcatgtc ggttaacgat actggaagtt ggttcttttt cataaacaca tagattttat
    11701 caaacaaatc gaactgctga tccatcatca ataaggtcag tacaccataa ctgacagatg
    11761 ctccgtgaag gtatccctcc attttttcta gaacagtcgt accgtaatag aatgagtgtg
    11821 ccatagcaga attatagtta ttgttaacta aaacagaaac caatccggta gtaacaatga
    11881 tatccgaaac gacttgttca attgcatatg agggacgatc gtcggcacta tctttcaatg
    11941 cttgcgtacc ataagtcaag agacgatcaa tgcacatttt tccgatttgg attcctaatc
    12001 tatccgtgta gcctagatca tcgccacgag cagaaaaagt agattcgact tctttggata
    12061 aagcatcgcc aatacctgcc cataagtatt tatcaggtgc ttcggcaata attgctgtat
    12121 caatgaaggt gtgtacaggt ggttttgaag gaaagaagag tccgtccatt acgccttctt
    12181 cgttatagat gacacatacc gcagtgacgg gcgaacagtt cgaaccaata gtgggaaagg
    12241 tgaagactgg tttagctagt ttttcaccaa tgacttttac agtatcgcaa gcacggccac
    12301 caccgactgc aaacaggacg tctgcttttt ggacactttc atgctggatc aactgttcaa
    12361 tatttccata agttgaatta cccccgtacc agagactggc gacaacctct atttcctgtg
    12421 tcaaagctac ttttattttg ttttctgcct ttgaaagtgc agtcttccct ccaatcagca
    12481 ctacttttgt cccatattct ttgacgattc catttgcttt ttcatacgca tctgttccaa
    12541 tggtataatt tggtaagaaa acactttgac tcatattcat ttccttcttt cttcgttgaa
    12601 acgggttaaa attttaaaga gtgatcgatc tgactgcggc tgtagatgga gtagaggtga
    12661 acctaagaca ggataagtga tgaataaggt ggcatgaaca ccttgaaatc atcaataagt
    12721 atcctaaaaa cgaacaagta caaaaacatt gaaaacgaca ataatgaatt tttgttcctc
    12781 ttagtatttc taccgattcc ctttgctttt ttgtaattat ctaaaaattc taaaaattaa
    12841 gattattata tgaaaacagg ttttatttgt caattcataa ttggtttttt ttaaagcaaa
    12901 agataatcta tatcatgatg aacaaacaga gttttttcct ccaattgatg gctttcctga
    12961 tctttcctat tttgaataaa gtgtttctgt ataatttttt tattttataa gtaagataaa
    13021 taatgaaaga atagagatac tgattggtag ctaaagattt ttgagcgaga acatttcgtt
    13081 ttttcaaaag gactgttaga ataagcaaga acaaggggga agacgtatga taatcagtga
    13141 atttgaccgt aataatccag tattgaaaga tcagctttct gatttactga gactgacttg
    13201 gccggaagaa tatggagaca gctcggcaga agaagtagaa gaaatgatga atccagaacg
    13261 aatcgcggta gcagcggtag accaagatga gttagtagga tttattggtg caatccctca
    13321 atacggtatc acaggttggg aattgcatcc attagttgta gaaagctccc gacgaaagaa
    13381 ccaaataggt actcgattag tcaattactt agaaaaagaa gtagcttcca gaggaggaat
    13441 cacgatttat ttaggtacgg atgatttaga ccatggaaca acgttaagtc aaacggacct
    13501 gtatgagcat acatttgata aagtggcttc catccagaac cttcgtgaac atccgtatga
    13561 attctatgaa aaattaggtt ataaaatcgt aggtgtctta ccaaatgcaa atggctggga
    13621 caaaccggat atttggatgg caaaaacgat tattcctcga ccaaattctc aataataaaa
    13681 agaggttgtg acagaagtaa tcagcttcaa gaaataagaa ggaatttacg aaaattactt
    13741 cttgaatttt tgtgaaattc caacttattt ccgaagaagc tacttttgga acaccgtgta
    13801 ccagaggttg tgaaaaagtc gcttagtttc gagtagtaag aaaaattttg tgaaaatggc
    13861 tttctacatt tttgcat
//
LOCUS       DAYYCY010000065        13841 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000065 DAYYCY010000000
VERSION     DAYYCY010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13841
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.065"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            370..1809
                     /locus_tag="ACHAZB_001515"
     CDS             370..1809
                     /locus_tag="ACHAZB_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8614194.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            2104..2322
                     /locus_tag="ACHAZB_001516"
                     /pseudo
     CDS             2104..2322
                     /locus_tag="ACHAZB_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
     gene            2571..2786
                     /locus_tag="ACHAZB_001517"
     CDS             2571..2786
                     /locus_tag="ACHAZB_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8614195.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(3003..3259)
                     /locus_tag="ACHAZB_001518"
                     /pseudo
     CDS             complement(3003..3259)
                     /locus_tag="ACHAZB_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(3446..4357)
                     /locus_tag="ACHAZB_001519"
     CDS             complement(3446..4357)
                     /locus_tag="ACHAZB_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM8614196.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(4376..5380)
                     /locus_tag="ACHAZB_001520"
     CDS             complement(4376..5380)
                     /locus_tag="ACHAZB_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFM8614197.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(5377..7500)
                     /locus_tag="ACHAZB_001521"
     CDS             complement(5377..7500)
                     /locus_tag="ACHAZB_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFM8614198.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(7505..9952)
                     /locus_tag="ACHAZB_001522"
     CDS             complement(7505..9952)
                     /locus_tag="ACHAZB_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8614199.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(9939..10328)
                     /locus_tag="ACHAZB_001523"
     CDS             complement(9939..10328)
                     /locus_tag="ACHAZB_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM8614200.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(10388..10891)
                     /locus_tag="ACHAZB_001524"
     CDS             complement(10388..10891)
                     /locus_tag="ACHAZB_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFM8614201.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(10904..11128)
                     /locus_tag="ACHAZB_001525"
     CDS             complement(10904..11128)
                     /locus_tag="ACHAZB_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8614202.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLSLFNRIVGA"
     gene            complement(11232..13142)
                     /gene="ltrA"
                     /locus_tag="ACHAZB_001526"
     CDS             complement(11232..13142)
                     /gene="ltrA"
                     /locus_tag="ACHAZB_001526"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFM8614203.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
BASE COUNT         3923 a         2889 c         2426 g         4603 t
ORIGIN      
        1 tggaattgaa tatcatagca atgtatttga aaagcaaatt ggaaaacaaa taggtgcaac
       61 accataaata tgaaaaaatt gggaaaaagt agaatggaaa tttgcttacg tctcacgaga
      121 tagctcacac tattaatctg aagaacaatg aagtcgactt ttagaaattg ctagaagaaa
      181 gtaaagcgaa ttatcactag ttactataga gtaagttcaa ataaagcatc tccaacttac
      241 atggttacat gaggatatca aatcgaaatt actcaagctt cctatggcta gagtcgcgag
      301 aagttgttgg gcagtagaca taggttatat tgttcttagc tacacgatca ttagaaagga
      361 aatcctatta tgtatagtta tcaagaagat gcaaaagtcc aaaaaaatcg ctggtggatt
      421 ttgattgcta tatctatgtt taccttcatg tccacattgg atggcagtat tgtcaatatt
      481 gcactaccta ccatctcaga tgatatgggc ataccaatga atcaagcgga atgggtagtg
      541 tccgtttatc tgatgattgt ctgttcttgt ttgttgctgt ttggtaaaat tggcgatagc
      601 cgtgggaaaa tcaaagtatt tcggattggt acgctgatct ttgtcttggg atcgctgttg
      661 tgtgggttca atcattcgct aggtatttta ttgttcgcac gggtggtaca agccattggt
      721 gctagtatga ctatggctac aaatgccgga atcatcacgg aagtctttcc catacatgaa
      781 cgtggtcggg cattgggagc aataggtgcg tttgtttcat taggctcgat tgctggacca
      841 ggtcttggcg gcttgatttt gtcccaattt tcttgggcgt acattttttg gatcaatgta
      901 ccggtgggaa tcgtaacgat gttgattgct gaaaaattct taccaaaaga tatcaccatg
      961 agcggccata caatcgatgg agggggcttt gctgccattg cggctacgat tatcagcttt
     1021 tttggagcaa tctttttggg gcaagaaagt ggctttttcc atccagtccc ccttgtactg
     1081 ttcgtaattt cagtgattgc ctttgtcagc tttttaaaag ttgaaaagaa agcagcctct
     1141 ccattgattt ctttcggtat ttttaagaac aagattttta ccatgagctt gattacagcc
     1201 ttgttgattt tttcttccaa cttctttgtc aatgtagtga ttccttttta tctgcaaaat
     1261 gctcgcgggt tgagtcctag ctatgctggg ctgttgatga tggtctttcc tttgatgatg
     1321 gtcgtcgggg cgcctttaag cggctacctg accgaccggg ttggcccgga gattttggta
     1381 ttagtaggct tgggcttatt gtcggtaacc caggtcatgt atatgttttt gtcagtggac
     1441 tctcctttgt ggctatacat tgtcgctact gccatcatgg gtgccggcaa tgccttgttc
     1501 cagtccccca ataatacgat ggttatgagc tcggtcacta tcgaaaactt gggactggca
     1561 ggctcgatga attcctttgc ccgaaatttg gggatggtca gcgggatttc gctttctacg
     1621 acggtcttat atcaagcgat gtctgctaag atgggccagc aagtaactaa ctatatcgca
     1681 aatcggccgg atgtcttttt atatggaatg aagattacct ttttaggcag ctttgttttg
     1741 tgttttgttg cctttttcct gactatctgg cgtatcaaaa aacaaggagg tattcagaaa
     1801 aatgtctgaa aaaaccactg ttgaagaatt tttggaggcc tgcgtatggg tgagtgggat
     1861 ttccatggat gatggaagat gaagtgaact tctaaaagcc gaaaaactca agtattatct
     1921 tcaaaatact agtgctcaat gagatgcttg atttgatcac aagaagactg cgaaagccgc
     1981 tggcaaccgc aagctaggta tcccaccgat ggactacgtt ttagaggcta ctggttatga
     2041 atacggagcc aacgctctca tctgcataag aatacatcac ccagattatg taaagatttt
     2101 cgatacttct gtcaaaaaat cattaagata gataaatgat ttccgtctag tcaaattggt
     2161 tcagaatgta gacacacaga tggtaagaag tcactcacaa ttcgagaatg gacttgtcct
     2221 atttgccgta tacatcataa tcgaaacgtc aacgatagta tcaatatttt gaccaagggc
     2281 tatgaataca agcattgcct tagaaaacat caagaacctt aggaacaacg gggatagctt
     2341 ggttaataag agataccgct gtttaggctt gcaagtatac tcttttatca agaattccgt
     2401 ggctttagtc atgagaggct taatactatg aaaattttct ttagcatttt tgatttaggt
     2461 tccttttcta attataataa gatgaccact acagtaacct actgaaatat ataaacagcc
     2521 actgtagtac cattttcaaa gtagtctatg agaggagaat cttcattctt atgaacgctg
     2581 gaattgcgga tatgaattta ataaaaaaaa cccttaatga ttttacttcc aattcaatat
     2641 caaaaggtac tggtatcaat ttaagcacaa taaaaaagct gaaaagtggt gaaagatcag
     2701 tggaaaagct gaatctttta gatgctataa aaataacgga atttgctatg aaaaatggaa
     2761 aagctgagat tgaaatatgg agataattat cgtctagtga attacttgcc acttagctga
     2821 atttaacgat ccttggtgct tgaaggggga gattcttggg aatagagcat acttgcaagc
     2881 ctatgtcttt actaacaacg atatctctta ttaaccaagc tatccccgta gttctaaggc
     2941 ttttactttt atctaagcaa taatcgtatt caaaggaaat ttttgaccct tagaaagtct
     3001 cattttatca gacgtgagct taggcattgc tgaattttgt taattcggtt ttaggttgtg
     3061 ctttgagcat aatatgaaag tattctttat cacgatttca ttcatctaag gtaatgtgat
     3121 gtgattctga aattctttca aaggcagttt ttgcataagc tgacattaca tcatcaatga
     3181 catgtttaca atgtttcact actaaaataa gggggtaacc aagaagaaaa actgaatgat
     3241 tatttgtatc taattccatg gtatatcaac catttcctat atagactgat tatgacatgg
     3301 attaaaataa aaaggcacta tgtcttaatt gtcggttttc gagttaccga atggcattca
     3361 tcacccacct atagaggatg ggcgacttct gcctgaaata agttacacta cttataactt
     3421 ctaataaaat tagattgagt cattattatt taacatcaac gattttccag tttccatttt
     3481 tttgtaatac taatttgtac tgaaagtagc tagtggtttt tgatacatta tcaaggtatt
     3541 ttatctttgt actcactaga aaattttttc cgttttgtag ataaactgga ttctctaact
     3601 ccgaaaactt taagtcaata ttgattggtc gcatcgcacc attttcaaca taatactcca
     3661 attctttttc agaagctgat ggataaagtt taaaaaatgt ttctaaaaaa tcggtgattt
     3721 cttccgttgt tttggcatca atttctgaat cgttttctac ttgttttgct tcatattttg
     3781 ccttgtctgg ctttccaatc attgttggca aactagttac taaatgattc ctgttttcat
     3841 cttcaaagat tgtcactcga tatgcagaag aaattgattg cgtctttttt ccctctgtaa
     3901 tatgttgctc cactaaaaat gaaacgacaa attcttccga gtgtttttca acatctagaa
     3961 tttttacaga ttgaacttcg gaactcgtgg gaatatctgc tcgaaccata tcttggatta
     4021 aagcgagacc gttttctgta aggtactgtt cgagattact cattctcttt tcaattgctt
     4081 ctttgttatt tttccatgaa aaatattctt tcgcaaaatc ctctgtaaaa ctttcaacgc
     4141 cagaagtatt cactaccttc tcttttataa ccactttttc atgagttgta tgttgatcaa
     4201 tggcggtgaa atttttataa acgccaaaag caagactaca agacaataaa agccaaaggg
     4261 caagcaccat ttttcgatgc ttacccacgg atacaactcg ttcttttttc ttttttgggc
     4321 tcttttcctt ttgattccgt tcaattttta tcttcattct ttttcctctt ttctcttatt
     4381 tgtatcggcc agctccgata agatgctgcc gccaatagtt ttccgttaga ttcgtccacc
     4441 ccactggatc tcctgcatga aacatgcgat tctcccctgc gtaaatgccc acatgggtca
     4501 cataagtacc ggcatcatag gttgaatgaa agaaaactaa atcacctggt ttggcatctt
     4561 ttagatcaat atgagtcatg gcatcatatt gatcctgagc cacccgagga agtttcaggt
     4621 caactttggc ataacaccat tgggtcaagc cactacaatc aaaccccgtt tctggacttg
     4681 agccaccaaa cacataggcc gttccttcat acttaaatgc ctcatcaaaa atttcttgga
     4741 ctattttgtc atcaaactga gttgtagtta ggtattgctt cactaataaa cagtaaaaca
     4801 tattgccata attataccgc cacccaccat tttccttaat cgcgattggg ttggggtaat
     4861 cgactttttg accacctgat ttttcctttg aaaaagattc agctaaagca aaactatgct
     4921 tcttcccgtt tttcccgaca tgatctagat acccgccacc atagttatag gcctgaataa
     4981 tactatcttg atcacaccct agttctttgg aacgcttaac caaagacgca aaatagagac
     5041 agccttgttt gatggatgcc tcggtactta atgaattagg tggtaatccg gctgactcag
     5101 aactttgcat aacgtcttcc attgtgccac cagattccac ttgaataatc gccaaaagaa
     5161 ttggaatata ctctgttata tccgattctt tggcatactt ttccaccatt gcacggtgtt
     5221 tcaatacttc ttctgagaca ctagttccgc caggagaagt gccagaacta ccatttccct
     5281 cactgtcttc accaaacaac aaacccacac acatcaaaat acccataaaa gagatggtca
     5341 gaaaagttaa tgaaataaca cccactttct tcaagttcat tttttctcac ctgaccttga
     5401 ttttggacga tggttcattc ttttttgtac tttttgattg gtctgctttg gcagagtgga
     5461 cctctgtttc acttgttgga aagaattttt cttacttgct gattggcttt tcccttttga
     5521 aatgatttct tgactagtta gttttcgttt gatttcggga ttcagatttg ctctcacaat
     5581 ccgctttggt gcttcttttt tcagcatcat atctttctga ggttgagctg ctttatctat
     5641 tggaatattc gcccttcttt ttccctgacg atttgttgct tcactcagta tcttgcgttt
     5701 ttcgtccatt ttcgcatttc ttcgttgtcg ttgtttttct cgcaaatcca ctcgatccgc
     5761 tttttcttgg aacagtccgc gcttgaattc ttctggtgct tttttcgact tttcgattcc
     5821 tttatggaga ttgtatttca aattcgttgg cgtattcctt acctgatcct ttacttgttt
     5881 agtcttatct ttcaccaaag cttgggtatc caaaactttg ccgatttttc tcccagccaa
     5941 attcattcgg gattgtttct tattatttga aactggattc tccttagtta agtcagatag
     6001 tatttcgtga tcgttcggta atcgtgaatt ctttcgtaga gaagaaccgg atggcttaaa
     6061 tttggatttt gattgttcct ttgccaactt atttccgact gctgcaccac caccaaaagc
     6121 caaagctcgt cccacatttc gattgagttg ccgcaatttt cgattcatta acatttgcgg
     6181 ttttcgcatg acgcgtcttc ccattgactg gctatcatta ctctggaggt tgaacatact
     6241 cataatgtcg cccaatttga aatagatacc ggcaaaggtc acaatttgca gaaaagcaac
     6301 catgaaaaat ggataggttg ccgaaatatt gaaaaacatc gtggagatgc taaatgcagt
     6361 cgtaatcacc aaagtaacac ccgctcgcat cataatcgta ttgaataaac gaataatcgc
     6421 cttctttcct aaactttcat aagtcggcaa catggacaat aagaaactaa ttggcagaaa
     6481 catcgcaaag ataatgaaga gaatttgcga gaacaacata atccctgtta gaagaaaaac
     6541 aaagaaggaa atgccaatat tgaacaatac caaaaagacc acggtcccta aacggttcat
     6601 ggtcttggta atactgagat tcgcattgtc attgtcttca atctctgcct tgaccgcttc
     6661 ttcccgatct ttgcctttgt tttcatctgg actcacagat agaatcttgt tgacccggtc
     6721 ttcaccaatt tcttctatgt tggaatcatc aaattgcagt agtaaccaag gttgttggac
     6781 ttgaatcgaa aacaaactat cccgaatcaa atccacacta tcttttcctt ggctttctga
     6841 attgggtaca acgatctcgg ttccaagggt aagggccgct tcactaacat ctgagctaaa
     6901 gtcattgatt tttgtgatat aggtaggcgc ataagcaatg aacgaacccg atagtagaaa
     6961 aatcatcaca aaattaatca cggctcgaac tgctttcgtg gtttctcgtt ttaataatcc
     7021 ggtatacgcc acatacaccc ccagagctaa aatcatcaat aatagaaacc caaaataaaa
     7081 tccggaggtc tggagtccat tttcagtaat accagccaat gtttggatat tctttccaat
     7141 actttcagcc gtatccgaaa tgaaatctaa tttgtaagcc tcttgaacca cataacccgt
     7201 ggcgttcgac aaatacaaac tcaccgtcca aatgaaatta gtaatggcat aaagaccata
     7261 catcacgctt ttccctaagc catcacccca attccaaggc aaccaatccc aagagctatc
     7321 cacaaagaaa tctagttgat agttgttcag tgaatatttg gaatatagat ttcctgcttc
     7381 aactgtatca tcaactagcc ctgtcgcctc ggctaccgta ccaactaaac tcaatagaac
     7441 tatcagagtg agtatgctaa taaaaacgat tcccacaatc cgaaaaagtt tctttttcat
     7501 cccctcactc cattctctct tgaacaggtg gacgggtatc aaaggcatgg aagagttctt
     7561 caaagactgg atgtacctga accacgccta cccgaccata caaatcctgc atcaagcatt
     7621 ggccattttc caaatctcgt aggcgttttt gattgctttc atcttctcga tccaagccaa
     7681 agaattccaa ggttttcttg atttcatgaa tatcggtact gcgaaacgca aacttcaacc
     7741 cgatattatt cttcaagcgt tcatccagta aatcatcact attttgcgtg acaaagtaaa
     7801 ccccggcatt catagcccgc ccagcacgaa cgagcttatt cgataatgtt ttcccttgcg
     7861 ctacttgaag aaaactccac gcctcatcta agtctacaat tttgaagatt tctcgattac
     7921 tatggataaa gtccaaggca aaagtggaaa tgacgattag catggaaaca ctaagcaatt
     7981 ccatggtggt atattcttca aaagtggttt ctgcatctgg taaaaccaaa tccgctactt
     8041 gaatgatatt taactgccta tctaaactaa tcgactgggt aatcgaacca tctgagaaaa
     8101 gcaaatgggc aaaatcataa tcggtaaagg actcgatgtg atccgcgata ttttccgcaa
     8161 ctggtgagcc ttcttttcga agctcattaa ttacttgtaa taatccccgt tgattgcttt
     8221 gggtaaccgt ccgaatggcc ttacgcaaca ctggaaactt ctccccatct cgactagaaa
     8281 ttcctgttaa aaaggtcaga atatcaattg ctagactttc agaatccttc tttttctcca
     8341 taatcacata aggatcaagt aatccttgat tttttggctt actagttaag ttgagaatat
     8401 tgatttcatc cgcaatttct ggcaaggttt ctttccagcc accccgttcc ccttttggat
     8461 cgacaatcac agcttgtcca ccaaaaagga cggcgtaata aactaagaga ttgttactaa
     8521 aggactttcc gccaccgagt gaaccgagga atgccgctgc tagcgcattg gtaactgaat
     8581 ccttgacccc ttgtgaggcc aatgccggtt tcaggtaaac attgcgcccc gtatctacgt
     8641 tataaccaaa gtaaatgcct tccaactccc ctaacatctg agtggcgcca aaaccaagtc
     8701 cggcgagaaa atcagacgta acatactgga tataatcatt catgtatcgc ttactagaag
     8761 gtaagaattc ttcatgtagc cccatcatat cgccaaaagg ccggactagt tttacattgg
     8821 tatcatcata aaaatctagc acttcatcac accggcgctt tagttcatct actgattcgg
     8881 cacttactcg aaccacatag ctcagtttgt acatggattc tttggactga tccaaggtag
     8941 tttccaattc atctacggaa tccaatgcat ctaagacatt ggaatttgtt tcattgtcag
     9001 attgataggc atggttgtcc aagtctttta attccttttt cttattgcga acggtagcaa
     9061 gtgccttttt gttcgtcaca atctccacat tcatactagt gtcaattggg aatgtaaatt
     9121 gttgttgctg gtaatagaaa agttctgagg agggaaactc catttccccc acaattgtat
     9181 taattgttaa gtacgctaca tatgattcat gattctcatg ttccatgcat aagtaccggg
     9241 gcttttcttc aatcaaacaa cgactgggac gtaataagtc gtaacgtttc accaaagttt
     9301 ccgacttcag ctttttcttt ggtagctgaa attcatattc ttcaaatggt gtaccctttt
     9361 cgccatagat atgttcaatc aaatacatca gatcactcgg tgtgactcgt ctgactttaa
     9421 atcttcgagc caatttactt tccattagtt tttcaagctt ggtatatcgg cgaatttctt
     9481 cattggataa ggaaacaaaa tcgcccatca aatgatgatt cactccatag acaaattctt
     9541 gaaagccgga gaagaaagat ttcttgagat tctttagatt gacctcttca tctgtcgcaa
     9601 tcagtttgaa gccaataaaa aagcgataat caatctgact atcgccaatc atggatacta
     9661 atgcctctgt ctgtaaatca attcgttgtt tcgctacttc tgttaatcgc ccggtaattt
     9721 ccttttttga tcgttcttgc gtggcacgaa tactactttc tgtggcaatt tgtaaggcat
     9781 gaatcttgcc ttcacgattt tgcgcaatca attgacgaaa gttatcgtgg acttgatatt
     9841 tctgttcagg tgacaaaaag gagtaattgt aaggtattaa ctcatagtag gcaaaacatt
     9901 ctccctcttg attaaagaca agattatttt caatatactt aatcgggtac atagttcaca
     9961 ctccttacca gagtaatggg ttcgtcccat cgtttcttct gttctttgac tttctttcct
    10021 gcgaaagtga ccttaaatcg taagtgatag ctaataaaag atcgtaaaaa tcccaatggt
    10081 ttcttaccgt caaacgtttt ttgcgacatg aaccaagtaa ctgccaccgg tatacctaag
    10141 tattttaaaa aggccccctc tatcatagat agtggaggca catgggcaaa tacgatgaca
    10201 aacagcaagg ataaaacaaa ccaagccatc tggttaaaag tcacaggaaa aggcagttga
    10261 aagtcattaa ttgcataaat gaccttttca actgcccaaa tactggtgta gctttttatt
    10321 ttcttcatca aatcattcct ttaaaaagaa ggatagttta gaaatcaact acccttcaat
    10381 aattttgtta atatggatac tcaaaaacac ctcgactcgt taccaaataa ttgcctccaa
    10441 tctccaagtc tctaccacac gcatcaaaat caatatacgt cttcaagtgt tctggtactt
    10501 ctccaaaatc accagcttct acaatcaaga aatatgctac atcactcata tcatcgcaat
    10561 ctggataata gataatatca tccacatgct ctaccatctc ctcaaaagaa ttgaagaaag
    10621 catgttgaat ctcagaagca acttcaccaa ctggcgttcc ttcaagttcc tccgctaagc
    10681 cacacaaccg attaatttcc tcaattggcg tatactcatc aatctcaaag ggaagttcat
    10741 aatcatgaat tgcatattcc tcgtattcgt catttaaccc aattcgttct ttcacttcat
    10801 caaaatcgac aggtggggta aaccacgccc ccaccaattc accttcgtta tatttcccga
    10861 ggttcgcaat ataaacacgc atctgttcca tcgtgcttcc cccttacgca ccgacaattc
    10921 ggttaaacaa actgagtaag acatccttca ccccagaagc attaaagacc aatccgaccg
    10981 caatcaaagc aatcaccaaa aagccaatca gtttggaaaa ctcccgctta aatcctaaaa
    11041 acaaaccaat gaccacaatt gccagtaaga ctaaggattg ggcattggtt aaaaaccatt
    11101 gatatatgga taggtaagtt cctgatatta taaatatctc aaacttttcc ccccattcac
    11161 acggagcgtg cgactttcac cgcactccgc gttccatcgt gtttatattg aaaagtattt
    11221 atgtagtaga cctatatcaa gtttaattct tgattgccag ctaatactct atatttattt
    11281 agtcttatga gctggacttt atttagcttc aatagagcta tatatttttg tattgtttct
    11341 tctgttttag cgtgtattag acggtgtacc gcctctttta ccaaaataag atttttataa
    11401 tcgtcatttc caccagctcc agttggtaca atatgatgac aatgtatttc attagcttgt
    11461 tgaaattctt ccagagttat agcacatttt ccatgttgag cagaaaatag cgaaattcga
    11521 ttgtctgcgt attctattgt gcgattttcc aaagataatt tcattaactg gtacatcagt
    11581 aatttatcaa cacttagatt ggtgtggata aattttcttc cctctagagt gtagcaattc
    11641 gctaaataca cctttgccat tggttttctg tgttggatat atccaacagg gaaaataggt
    11701 tcgccctcgc cagcaacata ccgaagcatt ttagatttac catatcttgt tgcctccttt
    11761 gtcgtaagtt gtctgcctgt ttttctcaat ctgccccttt tgtttgtacc taatctgtta
    11821 gtgaagatag ccattacagc tctattcagt ttcatacaat ccagatttac attcgttgca
    11881 atctgataat aattttgcat acctaatacc attgaattat aaagtcttgt ttcttctaat
    11941 tccgttttcc cttttgaggg agaagcaatt ttctttgcct gtgataccag cttgtctttt
    12001 tggtttttga attgtttatc acttatatgt gatttaacaa ctaacttctt tcttctcggt
    12061 atcattttaa tcttgaaacc aagaaagtcg gagtatcttt ttctaacatt tacaattctt
    12121 gttttttctt gtgatacttc caattttaat cgttgttcaa tccattgagt gattgctatt
    12181 ttggcttttt cagcactttc cttgtagcgg cagaaaactc taaaatcatc tgcataacga
    12241 accatataca tttcctttaa ctttgttttc ttcatttctt tgtaagcgtt gcttttacat
    12301 gggctaccgc ttttattgat atgaaccaca tagttttttg taacaggatt ttcctgccat
    12361 tggctttcta cccaatggtc taactcatta agaacaatgt ttgctagtag tggggatatt
    12421 attccccctt gcggagttcc tttctctgga tatgttattg taccgttagt cattttgatt
    12481 ggagctttta atatcctttt taaaatgaat atcaagtgct tgtcccgtat tcccatagcc
    12541 catatctgtt ttatcagctt ggaatggttc acgttgtcaa agaaaccctt aatatcaaat
    12601 tcaatcacat aatgtagctg gctgatttgt aggcggttat aggttgcttt tattgcattt
    12661 tcaactgacc tattaggtct aaaaccatag ctgttttcac taaatttagc ttcgcagatt
    12721 ggttctaata cttgttttat acattgttgt ataagcctgt cccacataca aggaatacct
    12781 agaggtctgg ttttaccatt tggttttggt atttcttttc gcctcactgg cttaggtcga
    12841 tacccatgtg gactgcctgc aacaatgtat cttacttttt tgacaactgt ttctgctggc
    12901 aatttgccaa tatctttaat tattgtaccg tctgttcctg ctgttttact gcctgcattt
    12961 gctttgatat ttcgatatgc cagtaatata ttttctctcg ataggataat atccataaga
    13021 tgagtgaaaa tatctccatt cttacttttg gcatataaat catcaaaggt gttttgcatt
    13081 tcgtaatatt cagcgtgtct taaatcttct acacacaatg tttcattttt atttttaggc
    13141 ataaggcttc actcctttac ttgaaattga gtgagctttt ttatcttact cgtaatcctt
    13201 attgaaacaa cataatactt ttctaatata tacacgactt gagggcattc ctccattctc
    13261 attacaagaa tttcttaggt tcgccctctg cttttcagca ttagttcaac tgcttattgt
    13321 tcttcgtcag tttattctct tgatgagaac ccaatacctt tcctcgttcc gataatctta
    13381 tctatacata tatatcctta ggtcactagc tataagcctg ctggcttgat accgcctgta
    13441 acgttatatg gcatttcata acctatattc ttactttgcc acaccagcta cgatttctcg
    13501 tacctttgca tttctacaaa gttcaaattt agacccgtac attcgctgtt tcgtcagttc
    13561 cttatagaac attctaacca tagatatttt atagaccccc gacctgttgg atacgctacc
    13621 cacctctgga tagttttcga tagaagatat atgttcatca actattacgg tatctttcag
    13681 ccgtctttac cgagcttcaa acattctgag tttcctcata caatgcttgt cggaggctca
    13741 gggcaggcgt ttcagagcgt tacttcatca ttccacctgt cagattatca gttctcctaa
    13801 taaccaaact ttttcgttct tttattagtg ccttagcttt t
//
LOCUS       DAYYCY010000066        13810 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000066 DAYYCY010000000
VERSION     DAYYCY010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13810
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.066"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(221..1423)
                     /locus_tag="ACHAZB_001527"
     CDS             complement(221..1423)
                     /locus_tag="ACHAZB_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8614204.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
     gene            complement(1426..1620)
                     /locus_tag="ACHAZB_001528"
     CDS             complement(1426..1620)
                     /locus_tag="ACHAZB_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFM8614205.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIIGVPLSQEE"
     gene            complement(1667..2515)
                     /locus_tag="ACHAZB_001529"
     CDS             complement(1667..2515)
                     /locus_tag="ACHAZB_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HFM8614206.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQGFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNALALNELGSPEQVMDFQESTVH
                     YTKIKK"
     gene            complement(2720..3070)
                     /locus_tag="ACHAZB_001530"
     CDS             complement(2720..3070)
                     /locus_tag="ACHAZB_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8614207.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            3248..4111
                     /locus_tag="ACHAZB_001531"
     CDS             3248..4111
                     /locus_tag="ACHAZB_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8614208.1"
                     /translation="MFGLVQPNKVKVGQRIKEIKDGMSLSFTELGNRLGLKKPTISSY
                     VQGYALAPKSVINQLSSISGKPVGWFYFGHVEEYIREYLFLIGQKKIVDDYPEIIEKI
                     KQEFYTGEFKNPAWENELGYPLEEFIQECFVEYSSEIMKDYVVQITKEVLEESKTLNK
                     LSSKAKEEAVTILSSGIIDYIEMSGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSY
                     LIGKLINILGTDEDTELFLSSLSMIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREK
                     TPDEFYDWFEK"
     gene            4735..5727
                     /locus_tag="ACHAZB_001532"
     CDS             4735..5727
                     /locus_tag="ACHAZB_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8614209.1"
                     /translation="MYWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            5743..6627
                     /locus_tag="ACHAZB_001533"
     CDS             5743..6627
                     /locus_tag="ACHAZB_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8614210.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            6676..8283
                     /locus_tag="ACHAZB_001534"
     CDS             6676..8283
                     /locus_tag="ACHAZB_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8614211.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            8284..9768
                     /locus_tag="ACHAZB_001535"
     CDS             8284..9768
                     /locus_tag="ACHAZB_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HFM8614212.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
     gene            9945..10937
                     /locus_tag="ACHAZB_001536"
     CDS             9945..10937
                     /locus_tag="ACHAZB_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8614213.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIDMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETVEKRTVLPIEFGEGDTTKPLVRNDFA"
     gene            10989..11858
                     /locus_tag="ACHAZB_001537"
     CDS             10989..11858
                     /locus_tag="ACHAZB_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005472675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFM8614214.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
     gene            complement(12278..>13810)
                     /gene="guaA"
                     /locus_tag="ACHAZB_001538"
     CDS             complement(12278..>13810)
                     /gene="guaA"
                     /locus_tag="ACHAZB_001538"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFM8614215.1"
                     /translation="KIIVLDYGSQYNQLITRRIREFGVFSELMSHRITAEEVKAMNPK
                     GIILSGGPNSVYDEKAFGIDEKIFDLGIPVLGICYGMQLITYKLGGKVKPAKNREYGK
                     AELEITSAEAALFQNTPEKQTVWMSHGDLVTQAPTDFDVVATSKDCPIASIQNAERKM
                     YGIQFHAEVRHSEYGNDLLRHFTLDVCQCTGDWTMDNFIDMEIAKIREQVGDKKVLLG
                     LSGGVDSSVVGVLLQKAIGDQLTCIFVDHVLLRKGEAEQVMESLGGKFGLNIIKVDAK
                     ERFLSKLAGVSDPEEKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQGTLYTDVIESGT
                     ETAQTIKSHHNVGGLPEDMQFKLIEPLNTLFKDEVRELGTQLGMPDSIVWRQPFPGPG
                     LGIRVLGELTEEKLEIVRESDAILREEIANAGLDRDIWQYFTVLPGIRSVGVMGDGRT
                     YDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVYDITSKPPATVE
                     WE"
BASE COUNT         4231 a         2337 c         2851 g         4391 t
ORIGIN      
        1 acctccagac ttcttgatag ctcaagtata tcttgaggtg catttgttag taaggtactg
       61 ttctatgggg cgcttacgtt gaaaaacgtt gatttatcgg cattccccat ccatattata
      121 tttacgtctt attcccactc aatatgagta gcctcgtaaa gaagctatta tatcagtgct
      181 ttttcagcct attcctcatt ttggtaggga aatggtaggg ttagattttt actgcttgat
      241 ttccattaaa gtttgcaact ttagattttg ttgcacaggt aaatttaatg tttcctgtca
      301 tttgttctgc aattccttca tcattacggc tccatagatg agcgtagtgc tttaaagtga
      361 tttcagggct agaatggcct aaccgttgcg acacgaccag aatgtctgcg ttaaactcat
      421 tgattaggta gctaacatga gaatgacgta atccttttcc ttgtattagg ggtaccttag
      481 ctagtttggc ataacgctga ataattcgtt gaacagttga acgatagagt gggaggtcag
      541 tgtagctaag aataaattgt tctacaccat gatttttttg aataactttc cactttttta
      601 gcacttcaat tgtatcatcg tccagagaaa tgatccgcaa gccgctttca gtttttgtgt
      661 aaggtttccg aataaaatct ttttgatttg tcatttccaa ggtgtgatga accctcaact
      721 ttttacgttt gaagtccacg tcattccaat taagtgctaa tccttcgctt acacgaacac
      781 cagtcatgta atatagccag atcattaaaa aacacatatg ttcgtagaaa tcatctttat
      841 aaatttttgc caaaacttct tcaaattctt cttttgtcca gtaaggaacg attgattttc
      901 cttttggaat tgcttttgtt cttttggaaa tattctcatt cagaaattgc agggtgaccg
      961 catagtctaa tgatttacga aacataccgt acataagtga actgtatgct tgtgagtatc
     1021 cgctatcgtt caataaccaa attcgataat tttcacaatc tcgcacattg atttcacgaa
     1081 gcttcttatc accgaaaaat tcagtaatct gaatcaatcc acactcacga gaactccaag
     1141 tacttttttg tacgctagct ttgtagtgtg gaacgtaaga ctgctccata aattctttgt
     1201 aagttaaatc ataattcgca aatccattta caaccatgta ttcatttttt atacgcatga
     1261 cttctttgtg tgcttcacga gctgatgaga atcgttttcc tagagaatct tttgttcctt
     1321 tcttctggat cctctttcca gtcacttgat ctgttcctaa ctcaactaga taagaaaagc
     1381 gaccattttt atcttgatac acacaagggt attttgtttt catagttatt cctcctgtga
     1441 aagaggaaca cctataattt cttcaattac ttgtcggggt actaccccaa ctcttttatt
     1501 attataatat ggatatccct gatttaccat tcttgttttt gcttgtcgga ttattgtggt
     1561 agcttgatgt gttttaaatc ctagagcctc taaatctttt cgacaaattg tatttttcat
     1621 tgtataaact cactttcaaa gaaaacacta tcattgggta atagtgttat ttctttattt
     1681 ttgtataatg aacggtactt tcttgaaaat ccatcacttg ttcaggactt cccaactcat
     1741 ttaaagctaa agcattgtaa aaaaatggac gtctaactat atattcgctt gtatatagta
     1801 tatcttgatt cgtggaatca tagggtttct ttaccatctt tttggtgaca gttggtagtt
     1861 tgagattccg ggacttgaaa aaagcttttt tcttatgctc tttcaattcc agctctttat
     1921 cgaaatattt gctgatatag cgcccgcgat tttcgatact gtctacatcg atcttgttga
     1981 tcttaacgaa accctgaccc caaatcgtag ttaaccggtt aaatgagata aaggggaaat
     2041 caaacaagat aatatggtag tggattgcac cgcgttgctg cttttcccac gtggcgagat
     2101 actttaaatt tgctttttta gttttataga ggtcataatt cagtcgtttg ataaattttt
     2161 taaattcatt atttgcatac tcaatgtctt gaatgttttc tttgaaagtg agagttaaaa
     2221 atttcgtttg attgtcaaaa ttcgtgtcta caaggcgggc aatctcaaat cgcatacttt
     2281 gataatgttt ctgtttgcgt tttaaactgt catattgtcc ttgagcggat aattcatcat
     2341 agttttttct tttttcaaga gtaatatcat tttcataaat ccaagctaat ttttgttttc
     2401 gttccagatt tacttcgttt tcgatcgttt tacgtttggg agtaatgatt gcttcataat
     2461 cataaatttc aatatacgtg ggagtttcaa tgattttctg attataagct tgcaaaaatc
     2521 atcactcctt gttgcgtatt cgattgtcaa agaaccagtc attttctcct gatgttgatc
     2581 tataaatcaa gttaaggggg aaggcctgcg tagcagccct tctagcctat ggggtgttgg
     2641 gcggtcttaa cggacagaaa cggctctgtg ggcgctgccc cgccgtttcg tcctaaccac
     2701 ccaacactcc atagtatagt tagttttgtg tagtggtacc ttgttttctg ggaatcaaag
     2761 ttgccgtatc agcggttgcc gataacccat aactgacaaa ttgtccattc gagcgtcccc
     2821 aaggtacaat caccaaattg gtaaatgtaa ttatggctaa accttgttgg gtaagttctt
     2881 ccggcgtgat cacaggatgt tcagatttga ctttgacact gattttttcg tagcgaagtt
     2941 ttggtaggat acattcgtag ctccaaccta gaatttcgcc ctccttttgc cagcgagtga
     3001 cattgacgac ttggtattct ttttcgagtg tttcttttgg taaactgaga tcggtaattt
     3061 ttattggcat cacaattctc cttttttata cgaaagttta tacgatttga ttaaaaaata
     3121 tagatggcca tctacgactt gtattatata cgagaactta tacgtagtca agagattttc
     3181 taaaaaaata tatacgattt tttatacgag tgttataatg aaaaaaatga taaggaggaa
     3241 tttgaatatg tttggtttag tacaacctaa taaagtgaag gtgggccaac gaatcaaaga
     3301 aataaaagac ggaatgtctc tctcttttac agaattaggg aatcgattag gtttaaaaaa
     3361 gccgacgatc agttcctacg ttcaaggcta tgcgttggct cctaagagtg tgatcaacca
     3421 attatctagt atcagcggaa aacctgtcgg ctggttttat tttgggcatg tggaagaata
     3481 tatccgagag tatttgtttc tcattggtca gaaaaaaata gtggacgatt atcctgaaat
     3541 cattgagaaa ataaagcaag aattttatac gggcgagttc aaaaatcctg cttgggagaa
     3601 tgagctaggc tatcctttgg aggaatttat ccaagaatgt tttgtagaat attcgagtga
     3661 aatcatgaag gactatgtcg tacagattac gaaagaggta cttgaagaat ccaaaacgct
     3721 aaacaaacta tcatcaaaag caaaagaaga agctgtcacg attctttcat ctgggatcat
     3781 tgactatatc gagatgagtg gtgagatcaa gtatggagag aaagatcgca ttgcgaaaat
     3841 ggtgaaagaa tcaataaaaa cctttgatgt gaaacaagac ctgacattta atgattccta
     3901 tttgattgga aagttgatca atattcttgg tacagatgaa gatacggagc tgtttctttc
     3961 ttctttatca atgatattta ctgataagca tttttccact cgatttggtg gggaagaatt
     4021 agtcgaaata tttcaatcga tgcgaccagc gttaatcaaa ctctatcggg agaaaacacc
     4081 agatgaattt tatgattggt ttgagaagta aaataagaaa agtggcttag attacaagtt
     4141 ggtgaaaaaa tttttttcag cataacatca gtaatctata aaggggttgt tgattatact
     4201 gtagaagtta agtactcaag agattttttg tacgtaaaca aagcaaaaaa gggtgcagtt
     4261 tcactagaaa atgtactctt ttttgtgatt tttcttagtt atagagtttt tttgaaactt
     4321 taaaaatgag aaaacatatt gattttaaag agtgtagaca tagcttcttt tgctaaatag
     4381 attaaactta ctggtgagtg gtcttgaaca tacttttaaa tcacatttta ctgagtttta
     4441 ttggatttta taattcattg gaaacctcgt acttaatctg tttttatcta tcgactagaa
     4501 tctgtatata ttccagctat caatgaggcc gcagaaaagg gtgcttaaaa ataatctgtt
     4561 gtttaaaata ttattatcag aatacttaga tacaaaattc tggcacaaag taaaaaaaat
     4621 attgacttaa aaaaatggat atggtaatct ataaacgaaa atagtggtaa cgtttccaaa
     4681 atgcatcttc gttaaatttt tattttttta aacgaaaatg gaaacgtttc taaaatgtat
     4741 tgggaggaga ttgaattgga aaagacattg ggaccaggtg gtgagataag ggcggtacaa
     4801 cgggataaaa aaaatatttc gaagtgggaa aaattaaaga aacagttgcg gcgtgataag
     4861 tggatgtact tgttgctctt accaggtatc atcatgacgt ttatctttaa gtatgtgcca
     4921 atgtacggtg ctgtgattgc atttaaagac tataatccta tgctaggaat tttggaaagt
     4981 ccttgggtag gactagatca ctttagggaa tttatcagtt caccaaattt tacgcttttg
     5041 ctgagcaaca cattaaaaat tagtatttat ggtttattgt taggtttttt tccaccaatt
     5101 attttagcac tgagcttgaa tcaaattatc aataaaaaaa taaaacagaa aatccagttg
     5161 attctatatg cgccgaattt tatctcagta gttattattg tggggatgct gtttttgttc
     5221 ctatccgcaa atggacctat taatgggtta gtagaaaagg ttacggggca accaattatg
     5281 ttcatgtcta atccagctta tttcagaacg atttatattt tatcgggaat ttggcaagga
     5341 atcggctggt catccattct ttacaccgca acgctggcgg gagtcagtac cgatttatat
     5401 gaggcagcaa atatcgatgg ggcaaatatt ttacaaaaga tttggcatat cgatttgcct
     5461 gcattgaggc ctgtgatggt gatcagcttc atcttggccg ccggcggtat tatgaatgta
     5521 ggatatgaaa aagcctactt gatgcaaact tcaatgaaca tccctgcctc agaaatcatt
     5581 tccacttatg tatacaaagt cggcttgcaa tcaggtgatt atgcctactc tgctgctgtt
     5641 ggtctgttca atatgattat taatgtcgtt ttattactga ttgtcaacaa tataaccaag
     5701 cggctaaacg atggtcaagg cttgtaatga aaggagaaaa ttatgaatag tgctttatca
     5761 aaaatcgacc gtcaaatctt atggttgaat cgagtgattg taactttttt agtcgtcatc
     5821 accattgttc cgctgattta tgtactggta gcttctttta tggacccagc tactttgatc
     5881 aataaaggta taagcttcaa tccgaaagat tggacaatac agggatatca acgggtattt
     5941 gcagatgatt ccattttacg cggttttgta aattcgttgt tttactcttt tagcttcagc
     6001 ttattgactg tatttattac gatgatcact gcgtatcctt tgtctcgacc taacttggcg
     6061 gggaaaaaga ttattatgac tttttttgtc attacaatgt tcgtgggtgg tggcttgata
     6121 cctacctatt tattggtaaa gaacttgggc atgttagata gtgtatgggc gattatcgta
     6181 ccaggtgcta taaatgtgtg gaatattatc ttagcccgta cctattttag tgctttgcca
     6241 aaagaacttt ctgaagctgc tactatcgat ggcgccaatg acttgcaaat actgttcaaa
     6301 gtcatgcttc cattagcgaa accaattatg ttcgtattgt ttttatatgc ctttgttggg
     6361 caatggaact catactttga tgccatgatt tacatcaaag atcccgactt acagccgttg
     6421 caactcgtat taagaaatat tttgatccaa aaccaacctt cacaagacat ggtgggagcc
     6481 aatactgcca tggcagaaat gaaacaaatt gctgagttga tcaaatacgc aacgatcgtc
     6541 atttctagtc tgccattaat cataatgtat cctttcttcc aaaaatattt tgataaaggt
     6601 gttttagttg gttctgttaa agggtaagta ttgttcacta tttgtatgaa taaaaattat
     6661 aaaggagatt ttaagatgaa agcaaaatgg atgctgccaa tcgtttttac ttccgtacta
     6721 attttggggg cttgtggagg ctcaaaagaa aagagtaaat caagccctga ttatgaacta
     6781 gaaaatgtct cttttccatt gaaagaaaaa gtatctttaa aaatgatgtc tcaaagtgcc
     6841 cctttggctc ccaatgatcc taatgacaaa ttgattttcc aacgattaga agaagagacc
     6901 aatctccata ttgattggac aaactacagt agtgattttg ccgaaaaaag aaatttggat
     6961 atcgctagtg gtgatttacc agatgccatc ttcaacgccg ctgcaggtga ctatgattta
     7021 ttaaactggg cggaaagtgg cgttattgta ccgattgaag atttgatcga agattatatg
     7081 ccaaatctga agaaaatttt tgatgaaaac cccgaatacc gacaaatgtc tactgcacct
     7141 gatggtcaca tttactctat gccttggatt gaagaattag gcgaaggaaa agagtcgatt
     7201 catacagtaa atgggatggc ttggatcaac aaggagtggt tggataattt gggtcttgaa
     7261 atgccgcaaa ccactgatca gttgatgact gtgttggagg ctttcaagac gcaagatcca
     7321 aatggtaatg gtgaagcaga tgaaattcct tttagcttta ttaacaatgg aggaaacgaa
     7381 gacttaaaat ttttaatggg ggcattcggt ctaggtgata atgatgacca cttagtcgtt
     7441 gataacgatg gtcaggtaca atttactgct aacaaagaag ggtataaaga agccatcaaa
     7501 tatttcaatg aaatgaataa taaaggtctt attgataaag aggctttcga acaagattgg
     7561 aatacgtatg ttgccaaagg gaaagaacat aagtatggcg tgtactttac ttgggataaa
     7621 gcaaatatca cgggtgcaga tgatagttat gacgtcttgc cagtcttagc gggaccagat
     7681 ggaacgaaac atgttactcg tacgaatggc atgggtttct caagagatcg tttcgtaatt
     7741 actagtgcga ataaaaactt ggaacttaca gctaggtgga tagataaaat gtatgaaccg
     7801 ttacagtctg tccaaaataa ctggggaacc tacggtgatg aagcacaggc gaatattttt
     7861 gaattaaagg atggtatgtt aaaacatttg cctttagagg ggacagcacc gggggaacta
     7921 agacaaaaaa ccgaagctgg aggaccatta gcagttcttg attcttatta cggcacagtc
     7981 actactatgc cagatgatgc gaagtggcgt ttagatttga tgcatgacac gtatttgcca
     8041 tatattacag aagacaatat ctatccacgt gtctttttag aaaaagaaga aacagatcga
     8101 ttagcaaaaa tcgaaacgga tatgtgggat tacatctatc gcaaaagagc agagtggatc
     8161 accggcggta aaatcgatga agaatgggaa gaataccttg ctgaattgga acgtgtaggt
     8221 ttggaagaat ggattcaaat aaaacaaact ggctatgata attttttgaa gggagcacaa
     8281 taagtgttag tagaagaaac ggcgattttg gataaagcaa atcgatttat tgaaatgatg
     8341 cgaggaaaag ttaaccctca gtatcgacaa aactatcatt tttccgcacc catcgggtgg
     8401 ataaatgacc ccaatgggtt tatatattac aaagggcatt accatctctt ctatcaatac
     8461 tatccttacg atagtcgatg ggggcccatg cattgggggc atgcgcgcag tagtgatttg
     8521 gtccattggg aagatctacc agtagcgtta gccccagatc aaccttatga cagcggcggg
     8581 tgtttctcag gaagtgcaat tgaaagagac ggaaaactat atttatttta tacaggctgt
     8641 caagaaattg atggtattag ccatcagcat caatgtttgg cggtctctga agatggtatt
     8701 cattttagca aatacagtgg taatcctgtg gtagcagaaa aacagctagg tgaaaatggg
     8761 ctaatacaag aatttcgtga tcctaaagta attgttcaca acgatggtta ctatatggta
     8821 gtagctacta aatctacaca gtctactggt agaatagtga tgtttcaatc gtcagatttg
     8881 tatacttggc acttttactc tgtgctgcta gaagggactt cagagcaagg aataatgtgg
     8941 gaatgtcctg atttattcca tttagacgga cgagatgtct taatcatgtc tccgatacaa
     9001 atcaagaaga acggactgga atatcataat atcagttcta ccatggcttg tattggtgag
     9061 gtaaactggg aaacaggtca gatgatagtt gataatttcc atgaaatgga ttttggaatg
     9121 gatttttatg caccacaaac aacaagagat cctaatggtg gacgggtgat gattgcttgg
     9181 atgcagatgt gggatcgtac attgcctaca catgagttag gccatctttg ggcaggctct
     9241 atgagtttgc cccgagagtt aagagtgaaa aaaaatcgat tggtacaaaa acccatctca
     9301 actgtatata gtcatttagt ttatcaacaa ggcagtgaaa atattgcagt gactgacaca
     9361 tcagtcgtat ttcgaaacgc gctgtctgat tcttcatatt tacatctagt cgcagatttg
     9421 gagaaagctg aagtttttac aattgaatta ctgcggtcta agacggattg tctcaaacta
     9481 gcctatgaca ctgctacaca ggtttttgaa ttggatcgtc agaaactagg aagagagctt
     9541 acaggggcgg aagaagaccc tctgaatgta cgaaaagttg aagtaccttt gattgataat
     9601 cagctcgttc ttgagatttt tagagatacc tcatcaattg aagtctttat taatgggtta
     9661 gaagttatga cggctacttt ctatgagctg gaaaaagctc gagatatctt gtttcattcg
     9721 atcggagaaa gtcgtattca agcttttgaa acagcgagtg tgaaataaat gtttgattgc
     9781 ttaggcgatg aatcaattat ttaataggtt ttgaaggata atgtttttag aaaagaacag
     9841 atagctagat ttcccatgga tttgctatta gtattttgaa aaaattagta atttatgaca
     9901 aaattcatta tactaaactt gtgcagaaat gtgagggatc aactatgaaa gcaacaatga
     9961 aagatgtcgc aaaattagca ggtgttggag taggaactgt ttcccgggta ttgaataacg
    10021 gttctgttaa agcttctact agagaaaaag ttgaagcagc aatgacagag ttaaactatc
    10081 aaccagattt ttatgcccgg gggttaaaga tgaagcatac gaacacggtt gctttgattc
    10141 tacccacggt atggcatcca ttttttggcg aatttgccta ccatgtagaa acagcattag
    10201 aaagacaaaa ctacaagctt tatttgtgta actccagtgg taatagtgaa aaagaacaag
    10261 aatatatcca gatggtcagc caaaataaag tagacggcat cattggaatt acttattcag
    10321 atatcgatga atacatttcg agtaatctgc cttttgttag tattgaccgt tatttcactg
    10381 aagaggtgat ttatgtgacc gctgacaatg cggccggtgg acgtttggct gcaaatgaac
    10441 tttatcgccg aggatgtcag aatgtagcat atatcggtgg gtaccaagaa acaccgaatg
    10501 aaacgaagag tcgtcgaaaa tattttgaaa tcgagtgccg tcaactgggg atttcctata
    10561 aagcgttaga tatgttagaa cctttagacc atctctcaga acgtgtaaca aattttttgt
    10621 tagagaatcc cgatatcgat gggattttta cgatcaacga tccaatggca ttagctgtca
    10681 ttgatatgct tgaaaaaata gggaaaaacg taccagagga tgtccaagtt atcggatttg
    10741 atggtcaaaa gatgacagaa ggagatcctt atctagtctc aacgattgcc caacctacaa
    10801 agcaaatggc ggaagtggca gtggcaaaat taattgctgt cattaatgga gagacagtag
    10861 aaaaacgtac cgtattgccg attgaatttg gcgaaggtga cacaacgaaa cctttagtga
    10921 gaaacgattt tgcttagtgc aaatacgttt ctttttttga aaatgagtga atgaaaggtg
    10981 tagataaaat gttatatggt ggaatagaag ccggtggaac aaagtttgtc tgtgggattg
    11041 gtaatgaaca attacaagtg atagaacgga aaagttttcc taccacaaca ccagaagaaa
    11101 cgatggagca agtgtttgat ttctttgata agtacaaagg aaatatcgca tcaattggtg
    11161 taggagcatt tgggcctatt gatattcaaa tcgaatcaag aacttacgga tatatcacca
    11221 acacaccgaa gttagcttgg caaaattatc attttgtagg agctttggaa aacgccttgt
    11281 cggttcccat ctattggact accgatgtaa atgctgcttg ttatggagaa tatatggcag
    11341 ggtatggaaa aggtaaaaat agtgttctgt attacaccgt tggtacaggt gttggtggtg
    11401 gtgctgtagt ggatgggaaa atagtcgcag gttttagtca tcctgaaatg ggacatatgc
    11461 tcttacaacg tcataatgaa gatcaatttg aaggaatctg tccttttcat catgattgtt
    11521 tggaagggtt agcatcagga cctgcgattg aaaaacgctt tggaagaaaa gcccagacta
    11581 tctcatttga tcatccttat tggacgattg aggcagatta tcttgctcag tgtgtgtata
    11641 acacaacgtt actatatgca ccggaagtaa taattttagg cgggggtgta atgaaacagc
    11701 cgcaactact cgagttgatt cgccaatcgt ttaaagaaaa aatgggttcc tacgtggata
    11761 cgcctactct cgaagaatat ctactaactc caaggcttgc tgacaatgcg ggcttgattg
    11821 gctgcctagc tttagcaata aagaaactca atcattagaa tagtatttat tagaactatt
    11881 cataacatac cggaagttta aaagggtatt ctgaaactta aatagtctca attcattctt
    11941 ctttcttgag aatctgagta gagactttta ttctgtttca aattaatata attacgaaat
    12001 gatattgagt acaagaaagt tctttttacc agatctttta aatcgaaaaa tctgatatat
    12061 atatttatag tactaaaaat ctgattagat aaaaagctaa ttcaatagga aaataaaggt
    12121 agtgaataaa agcaaagccc ttgttagtag ttaatctaac aagggctttg gtagggaaaa
    12181 tggtaggaaa ccatcttatt ttttacgaaa atcaggaact ttagtaattg aataatgttg
    12241 atttataggt attcctcatc catattatgt tggctcttta ttcccactcc actgttgcag
    12301 gtggtttgct ggtaatatca tacacgatac ggttaacgtg tgcgacttca tttacgatac
    12361 ggacactgat tttttgcaag acttcccaag ggatacgggc aaagtcagcg gtcatgccgt
    12421 caatagaagt gacagcacgg atacctacag tgtagtcata cgtacggcca tctcccataa
    12481 ccccaacaga gcgaatgcct ggtaggacgg tgaagtattg ccagatgtcg cgatctaaac
    12541 cggcattggc gatttcttcg cgcaagatag cgtctgattc acggacgatt tctaattttt
    12601 cttctgtcaa ttcaccaagg acacgaatac ctaagcctgg tccagggaat ggttggcgcc
    12661 aaacgatgct gtcaggcatg cctaattggg tacctaattc acgtacttcg tctttgaaca
    12721 atgtgttcaa tggctcgatc agcttgaatt gcatgtcttc aggtaatcca cctacattgt
    12781 gatgtgattt gatggtttgg gcagtttcag ttcctgattc gatcacatcg gtataaaggg
    12841 ttccttgtgc aaggaaagaa acgccttctt caccggcaag tttggttgct tcatcatcga
    12901 agacatatac aaattcattt ccaatgattt tacgtttttc ttctggatca gacacgccag
    12961 caagttttga taagaaacgt tctttggcat cgacttttat aatgttcaag ccaaattttc
    13021 cgccaagact ttccataact tgttctgctt cacctttacg caacaatacg tgatcaacga
    13081 agatacatgt taattgatcg ccgatcgctt tttgtaaaag aacaccaaca acagaggaat
    13141 ctactccgcc tgaaaggcct aataaaactt ttttgtcacc gacttgttca cgaatttttg
    13201 cgatctccat gtcgatgaag ttatccattg tccagtcacc agtacattgg cagacatcta
    13261 gtgtaaaatg gcgcaacaag tcatttccat attcggaatg gcgaacttct gcatggaatt
    13321 ggattccgta cattttgcgt tctgcatttt ggatcgatgc gatggggcaa tctttgctgg
    13381 tagcaacgac atcgaaatca gtcggtgctt gggtaactag gtcaccatga ctcatccaaa
    13441 cggtttgttt ttcaggagtg ttttggaaaa gggccgcttc tgcagaagtg atttccaatt
    13501 ctgcttttcc gtattcacgg tttttggctg gtttaacttt tccgcctaat ttataagtaa
    13561 tcaactgcat accgtaacag attcctaaaa ctgggatccc caaatcaaaa atcttttcat
    13621 caataccaaa ggccttctca tcataaacac tgtttggtcc gccagaaagg atgattcctt
    13681 tagggttcat tgcctttact tcttctgcgg taatgcggtg gctcatcaac tctgagaaaa
    13741 caccgaactc acgaatacgg cgagtgatca gttggttata ctgactaccg taatcaagta
    13801 cgataatttt
//
LOCUS       DAYYCY010000067        13680 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000067 DAYYCY010000000
VERSION     DAYYCY010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13680
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.067"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            138..476
                     /locus_tag="ACHAZB_001539"
     CDS             138..476
                     /locus_tag="ACHAZB_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8614216.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(681..1256)
                     /locus_tag="ACHAZB_001540"
     CDS             complement(681..1256)
                     /locus_tag="ACHAZB_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HFM8614217.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGF
                     YEWNEEKDKFMFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRMPLVL
                     TEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(1988..2569)
                     /locus_tag="ACHAZB_001541"
     CDS             complement(1988..2569)
                     /locus_tag="ACHAZB_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8614218.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(2752..3378)
                     /locus_tag="ACHAZB_001542"
     CDS             complement(2752..3378)
                     /locus_tag="ACHAZB_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFM8614219.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(3400..4731)
                     /locus_tag="ACHAZB_001543"
     CDS             complement(3400..4731)
                     /locus_tag="ACHAZB_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HFM8614220.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(4886..5287)
                     /locus_tag="ACHAZB_001544"
     CDS             complement(4886..5287)
                     /locus_tag="ACHAZB_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFM8614221.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(5373..5579)
                     /locus_tag="ACHAZB_001545"
     CDS             complement(5373..5579)
                     /locus_tag="ACHAZB_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8614222.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(5674..7176)
                     /locus_tag="ACHAZB_001546"
     CDS             complement(5674..7176)
                     /locus_tag="ACHAZB_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HFM8614223.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(7194..7649)
                     /gene="argR"
                     /locus_tag="ACHAZB_001547"
     CDS             complement(7194..7649)
                     /gene="argR"
                     /locus_tag="ACHAZB_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFM8614224.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(7747..8364)
                     /locus_tag="ACHAZB_001548"
     CDS             complement(7747..8364)
                     /locus_tag="ACHAZB_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFM8614225.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKPFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQLVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            8585..9958
                     /locus_tag="ACHAZB_001549"
     CDS             8585..9958
                     /locus_tag="ACHAZB_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8614226.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            10033..11145
                     /locus_tag="ACHAZB_001550"
     CDS             10033..11145
                     /locus_tag="ACHAZB_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM8614227.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWSTEDYQKDISTYITKNPS"
     gene            complement(11217..12854)
                     /locus_tag="ACHAZB_001551"
     CDS             complement(11217..12854)
                     /locus_tag="ACHAZB_001551"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFM8614228.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(13022..13630)
                     /locus_tag="ACHAZB_001552"
     CDS             complement(13022..13630)
                     /locus_tag="ACHAZB_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFM8614229.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         3686 a         2884 c         2452 g         4658 t
ORIGIN      
        1 ctaaaaaaat aatccaagaa acattgaacc tatggatcca aaagctaccc aaatactata
       61 gctaaccccc cgctaccttc aattttgcag ataaaacgct ttgggagaaa atttcaaaga
      121 caacaggcca tcctatcatg cccgattata aacaaaaagc tggctattcc ttttgctctg
      181 acaaatttca attgggaaag ttcacgaaac tagcaggtga agaagtttca actgttcgga
      241 tcaaagaatg cccgcttcaa atagaaacat tggttcaacg aatttcaact agacaagact
      301 ttgctattgt agaacgtgag atcgtagcta tttttgtaga agaagggatt ttacatgatc
      361 caactcatat caatgtggat aaatggcaac ctcttatcta taaattcaga gaatatacaa
      421 ctgccaacca gtcgttaact ttgaatttta gattccaaga attcaaagag tcataatgat
      481 aaacatcgtt aggtataaat aaaaaagact cgcagcacga gtaaaaaaca aatagaagct
      541 atacattaga aaggtcgact gtgaccctat agatgcagta tatgcagctt atgagcattt
      601 cttctgttct ctttgttttt gggatctgtg ctgaagtctt tttcgttttt tgccgctctt
      661 tctttgaaaa atcgattatg ttataccttc tccattacta attcaggcat ctgctgagta
      721 atgatttttc gcgcaaaaga aatatctgtt acccaaggtt cgatttgttc ttcagtcaag
      781 acaagcggca ttcggtcatg gattggtgcg actgtgcggt taggttcagt agtcattatg
      841 attgattctg ccattttttc atcctgatgg ctacgataaa aaccgccaat atacactatt
      901 ttttccttat ttgaaaacat aaatttgtct ttttcttcat tccactcata aaagccactc
      961 attgggaaaa cacaacgatt ttgcaaaaat ggttgatgaa aggtcttttt ttcttctact
     1021 gtctctgcac gggcattaat catcaattgt ccttttttaa atccggtaaa tccccacttc
     1081 atgatttccg gagtgaggat gttttcttcc tcttttctta acgtcacaac aagatttgac
     1141 ggaaaaactt ctccagctgc taattctgct gcctgcttcg gttgtttcgt ctgggcttct
     1201 cgataataat tctgaaggag ctctgactct agatcaaaaa aataacgacc acacatccta
     1261 atccctccca atcttttttt ctatcataca ctgcaacaag aagttcgcac caacaaaacg
     1321 ctttcaattt ttgtagaact aaaaaagttg tgacaagtga caaactctcc agtgaatggt
     1381 attctcctgc caactcctcg gtcacaagtc acaatattca acaaacatga gaagctgctt
     1441 tttgaaattg ttccttgtga ctcggagtga aacggcaggc ttacgacctc tgggtaaacg
     1501 gtgttcaaaa gccagtttct cggtattaag ccaaatttat gcaaaaatgt agaaagccat
     1561 tttcacaaaa tttttcttaa tactcgaaac taagcgactt tttcacaacc tcttccttaa
     1621 acggtgttca aaagcttgca tctgcggtaa taagcccaac caaacgaaaa atatgagcag
     1681 ctatttttat ttggctgttc ttattacttg atgcagggca gctttttcac aacctctcca
     1741 ctaaacggtg ttcaatcagc taaccttcgg tattagctca aaaaacggca aaatatgagg
     1801 aactattttg ccgtttgttt ctctaatact caggtcaaag cgctgatttc acaacctcta
     1861 atacacggtg ttccagaagt agcttcttcg aaaaataagc cgaaatttca caaaaatttg
     1921 agaagcaatt ttcgtaaatt ccttcttatt tcttgaagct gaccacttct gtcacaacct
     1981 ctaattatta aagaaccttt tgcgaagtta acaatctttc tttgatcaat tgaaaattct
     2041 gcctaagtag tgatagatcc tgtgtgactt tcaacaataa gatacttccc tggatttcag
     2101 caatcaacgc tttggcttga cggtctgctt cttcatcgga cagtttttct tcagaaagtg
     2161 ccaggatatt ttgtttcgta tacttgaccc attgattgac taattctagt agcaacagac
     2221 ggtattcttc atccactgta ctcaactcaa taatcaaatt acctacagga cagccatgat
     2281 aagtgatctg tgtctcatga tcagcgaaaa tccagtccaa catttcttct aatgcttctt
     2341 ctggctgttt tccagcattt aagatgtctt cgaacaattc tgtctgccat acgtccatat
     2401 gcgcatggat caccgctaga caaacttctt tttttgaaga aaagtaataa taaaattgac
     2461 cttttcctgt atcagaagct gctagaatat ctcggattga cgtttcatga tagccttttt
     2521 tataaataag atctttggtc gcttccaaga tcttttcctt tgttttcaat gatattctcc
     2581 tttttcattt ctcatagtcc tatattatac agtaaattca tcattttttc ttcttaatcg
     2641 tttaaaaaag actgtgccaa attatctgac acagccctca ctataaaact gttctcatat
     2701 agctagatgt tcattgattc aagaactgcg aggacaaaag caattcctat cttatgatag
     2761 ttgctctaat acttcctctg ttgtcatcac ttgatctgca ataaagccaa agttcgtcaa
     2821 cgctgcatcc attgcttctt ggccaggagc accaattgca tctgtcgcta ccacaacatt
     2881 gaatccactc tctaccaatt cacgcatatg ggaatcgcta cacaagtttc cgttcattcc
     2941 accaagaatt actgtgtcga ttccattttt tctcaattgg agcgccaaat cattgctttc
     3001 tggtccaaag attttatgag gagagctgat gatcgtattt tcatccaaat aaggtttcaa
     3061 ttcttcgata aaatcggctc ctgaaccttc ttccactgat tcatattgac tttttctagc
     3121 aaacatctgc tgctcaagca tcatatgttc tcctgcaccg ccaaattgcc atttatggtc
     3181 atgtgggtaa aaataatgag gagaaataaa gagtttgtat cctttttctt ttgcttgttt
     3241 taccatttca atcatatttt cgatcgtatt gtatttttct aagatatctt ttgtcaaacc
     3301 aaagccaccg cctgctggtt tcaagaactc gacttgcggg tcagtgagaa ctactgctgt
     3361 atttttatta ttgaacatgg tggattctcc attcgttatt tattttaata catcatttag
     3421 agcttcactc attgtcggat gagaatagat ttgatctctc aataacgtat aaggaagttt
     3481 tccattcata gctagcgaaa tcaagttgat gacttcatga gaatcttccg catagatact
     3541 tgcccctaga atcaagtcag tttcagggtc gactaaaact ttgaatacac ctttaggatc
     3601 ttctaatact tgtgctttag ggacaccgct tgtcatatac ttgaataatt tgtaattgac
     3661 gccttgtgct ttggcttgtt tttctgttaa acctacattg gataaagtcg gagtgataaa
     3721 tacactgtaa ggcacagctt tccggttatt cgttgttcgt ttattctcgc ccttcaactg
     3781 atccataatg attcgataat catctagaga gatataagtg aactgcaatc cgcctttgac
     3841 gtctccaatc gcccaaatgt tgtctgcatc tgtacgaagg tattcatcca cagcgattgc
     3901 tccgcgatct gttagtttaa catctgtatt ttctaatcca agtgatgctg tacttggttt
     3961 tcttccagtt gcagccaaaa cttttgacgc ttggattgtt acttcttggc catcttctgt
     4021 aaaggtgacg gcagctgttg attcttggtc tgctacttga tctacagata cacctagatg
     4081 gaattcgatt cccgcatttg tcatgtcttc taaaatcatt tgcgcgatat cttcatcttc
     4141 acgtggaaga aattcagaat gggcatcaag aacgacaact ttagatccgt attcattgaa
     4201 cattgatgca aattccaagc caatatatcc agccccgatg atcactaatt tttccggtaa
     4261 ttgttcctga tccatcgctt gcgtagaatc aatcaagtag cgacttgtat tcaatccttt
     4321 gattggtaaa atgattggtt cagaaccagt attgatgaaa atccgttcgc cttcaacaat
     4381 cactttttca ccattattcg tcacttcgat ttgatggtta gaaagaaaac gtgctgtacc
     4441 gtccatcact gtcccagttg cctcatcgct gatcatatga taatttttgg cgcgaagcat
     4501 gcccgtcaat ttttctttcc gagcaacagc ctctgtaaag tcgattcctc tttctccatt
     4561 gaaaatcaat gatttagaag gaatacagcc gatgttgata catgttccgc catacatacg
     4621 tgttgacttt tcaacgacta aaaccgattc accttttgaa gctaacgcct tcgctaaagt
     4681 cttaccgccc tttccaaaac cgatcacaat attttgatat ttttccatca tttttccctc
     4741 gcttcttttt tacctaccga ttggtacaaa aatatgataa ccgataattg tctattctcc
     4801 aaatcatctg ctttatttgt caaaaaaaac cagacaaaaa gcggtccttt cacgttttta
     4861 gtctggttcg atttccttta ttcttctaaa caatcgttac ttctacatcg atatttcctc
     4921 tagttgcttt ggagtaaggg cagaattcat gcgcatcttc tgcaattttt tgggcttctt
     4981 cttcgctgat gccatcgata tgcacttcta atactacacc gatctgatag ccgccatcat
     5041 ctaaattata aagagagaca cgtgctttta ctgtggactc tgctgtcatt cctgcttctt
     5101 tgatcatatg ttgtaccgcc ccattgaaac aagaagcata acctgctgca aataattgtt
     5161 ctggatttgt tccttctgca tggattcctg gaggagtgat tttcatatgc atcgagccat
     5221 ttggtgcctc gacttctcca ttgcgcccgc catggttgat cattgctgtt tcgtatgctt
     5281 ttttcattgt actgctcctt tcgattgacc atttgctact tggctataat tatagtgtat
     5341 gacaagtaag gcgctttcta caaaaattct gcttattcga ataccagaat gccgtcttct
     5401 actatttttc caaaaaacaa aaaaatacta ctcgtgaaca acagcgtcat atttgttcca
     5461 aaataaatag caatcttttc tacataatct tttatatgat tgattttaag atacatatat
     5521 accagaaagt tgaccccata aaccaatact gttaaaaaaa taaccatatt cgttaccatt
     5581 ttttcctcct tttgctggtc tccctaccag cactcgattt aaccatacca ctttttttgc
     5641 cgtccaagca actagccttt caaaaagact ggtttattct gctgttaatg attcaagatc
     5701 agaaaaatgt ccgaataatt ttgctaattt tactaacgcc aacttgttct ttccgaattc
     5761 atcattttct atctgtaata ctagtaacgg ctcatgtaag cttaaacgga tcaaaaacca
     5821 gccgtttccg tatggctctt ctagatcata tcgaatacct tcttcatttt ccgggtcgaa
     5881 gttcatccct ttttcttctt ccaacagtcg cttcatttct tctaatatct tgtttccttg
     5941 ttcgcgtggt tcatcgctga acaattttaa tcggatttct tgtacttcta cgggctgttt
     6001 caactctttg atttgttcat ctaatgtgat accttttctt tttaattctg gcaaaagcat
     6061 caggatcttt gcgatgacat atgcaccatc atccagaaaa tagttttcct tgaatgcagc
     6121 gtgtccgctt gtttcgatgg ctagctgtgt atctttccca agacggttaa gttctaatgc
     6181 tttattgatt acatttcgat agccggaaat ataacgaact tggatgcctc ctttttcttc
     6241 aatgaatctt tgtagatgag aagaagtagg ggaattcgtc acgattcttg ttcctggatg
     6301 ctcttttaat acgattgtac ttaatactgc gatcaaatta tttcgattga tcactgttcc
     6361 agaagcactg acaactgcag aacgatccac atctgtatcg aaaatgatcc caagatctgc
     6421 ttcatttgct aaaacagcct gcttgatact ttccatcgct tctttattat caggatttgg
     6481 gatatgatta ggaaatcggc catctggttc caaaaattgg gaacctgttg aatccgcacc
     6541 taagctattc aatacttttt cagcaaagaa tccaccagca ccatttccgg catcaacaat
     6601 gattttccaa ccatttagtg gtttgctttc tagtgttcca atcccctttt taatttttgt
     6661 gactaaatct tgcgcataag gtgtcaaaag gtctgctttt tctacgcttc cagtttgttc
     6721 gctttttatt ttttgctgct gtgataggat aaaagcaata tcttcttttt ctgctcctcc
     6781 ttctgaggta aagaatttta ttccattata ataataggga agatggctag cagtcagcat
     6841 aaccgcagca tcgcacgaga actgttcaaa ttgagtggac ataaacatag caggcgtagt
     6901 agctaattcg aaatcgatga catgtatccc ttgttctttc agactttgga tcaacgcctc
     6961 tttcagtgct ggtccgctta tcctactgtc atgaccaatc ccaatcgtcc attttttatt
     7021 tggatcggtt gatttcttct ttttctttat ccagtttgct agtccccagc cgatcttttt
     7081 taccatgtct gtcgtcaagt ttacttcata tccttcaact ggtaatgcga ttcctcgaat
     7141 atccgatcca ttttgcagcg ctttcatatc tttcatataa aagtcctcct catttattcc
     7201 ctcatttttt ccagcttttc tgcaaatcgc tgtgcttctt cttctgagcg gcaaataata
     7261 atgaatgtat ctacaccggc catcgttccg acaacttcag ggtaagccac ttcatctagc
     7321 caattcgtta ccacatcggc accatttcgt tcagtcaata cgataaccat gaactgaaca
     7381 gactggatac gcaaaacctc gcgtctaatt gctgtacgca aacgttcttc atttaaagaa
     7441 acagatggct ggttaaataa tgtatagcgt actattttgt tttcattttg actttttact
     7501 aacttcattt ctcgaatgtc tcgagaaatc gttgcttgag tcacatcggc gcctgcttca
     7561 tgcagccgcg caagcagctc ttcctgtgtc ccaataggtt gttctgtaat gatctggcgg
     7621 atcagtgctt gtcgctgttt ctttttcata ttagacacct ctcaatgttg attatagcaa
     7681 aagataagta atatttcttc atattttggt attttttact tttatttttt gttttcattc
     7741 aggttgctaa cctttttcta ttttaacacg caaaatcgtc ttcatttttt caggtgctga
     7801 ttttcttgga tcgctgatgt aaatctcttt gtgttcttta gaagctcttt catagccttc
     7861 ttttgcgatg aatgcttcta atttctcaaa cgttgctggt tcttcggcat agggcccgac
     7921 gtgtaagatt tgggcacaaa gaccttcttc catctttttg aatactagtt gttctttcaa
     7981 gtcttccgga agtttgctag aacgctgaat ggcttcttgt gctatcgctt ctgtaacaaa
     8041 gtcaggctgt cggatcatca attgataaga aaaatgttct tttttcatca ctggttcatg
     8101 caaatacttt tcttgcagtc cccattgtcc ttccagtgga tagacagtat acatctgata
     8161 atcaggaatc agccaatttt ttttaggcga catgcgtatt gcatagctga ctgcatatag
     8221 acaggctact cttcgtttaa agtcttcctg attcgggtcg ccgatccctc tgatgctaaa
     8281 gaatggtttt ttaggaacct ctagtatttg cgggcttttt ttcatactat agctcttgtc
     8341 tttacgccat tcaatttttt ccatttttct tcctccgcga aattccattt agttatttat
     8401 tatcatacct ctttttgagt gacttggcta atcgtgttga cactattttt cagtttgtgc
     8461 tagaataagt gaagtaaata aatcgtacag ataacacgtt tcggcgtgtc gagagagagg
     8521 cggtttctat ttttataata gaaatcactc tcttttttta cgaaattttt tagaggtgat
     8581 gtaaatgaca aaacaaaaac agctgctttt agtacctgca gtaattgcaa cagggatgat
     8641 gtcttttgcg ggcgtattga tcgagacggc catgaatgta actttcccaa ctcttatcag
     8701 acagtttgat ttaacgactg cacaagtcca atgggtaaca acgatttatc ttttgatgat
     8761 ttcgatcatt gttccgctgt ctacttattt gaatcgcaat tttagtctca aaaaactctt
     8821 tttagcttca aatctgcttt tcttggcagg tataatgatc gatttcttct ctcctacctt
     8881 catggtccta ttgtttggtc gattgctcca aggtgcagcg acaggtatcg ctcttccgtt
     8941 gatgttccat attatcttga catttgcccc cctcaatcga cgaggcgcga tgatgggtgt
     9001 cggtactttg acaacggcca ttgctccagc aattggcccc acttatggag gcattatgac
     9061 ttcacatttt acatggaatc atatcttcct gtttcttgtg cccgttttga ttctttcttt
     9121 gattatcggc tggtttgcta ttcccgagat ggcagtacaa aaaagtggtt atttggattg
     9181 gataagcgtt ttaggaattt gtcttttgtt tagtggaagt ctaacatttt tgagtacgtt
     9241 gggaacagtt tccagctgga ttgctttaat catcggcggt attggctggt tcctttttta
     9301 ctatcgaagt aaaaaagtgg agcatccact gatccgactg tctattcttt caaataaaac
     9361 attccgcttg tttttgattg gcttcttagt ttatcagttt ttattacttg gtgtatcttt
     9421 tgtcttgccg aatttccttc agatcgttca aggaagtagt ccttttgtcg ctggattagc
     9481 gatgcttccc ggcgcagcgg ttggtgcttt gctttctcct ttttccggga aaatgctgga
     9541 tcaatatggt ccgaagaagc caatttttgc tgggctgatt ctttctttga ttggttggct
     9601 agcattattc ctactcatag gacacgcttc agtgggtgta ttgattgctt gtcatgtgtt
     9661 ttatatgatc ggtatcggat tatcttacag caatatgatg accaccggaa tgaatgcact
     9721 gaatgaagaa ctacaaggag acgggaacgc aatgttcaat accttgcagc agttttctgg
     9781 cgctgtggct acttcattag tagcggtcat tatcaattac gtccagcatc atacttccca
     9841 taactatgaa gtgtctacta cattaggatc aaaagcagca ttaggcgtct tgttgctgtt
     9901 gcttctcgta agctttgctc gattcgccta ttatctgttc tttgctaaaa aagcgtagat
     9961 tccatatacg gcaggtgttg catctgccgt ttttttgtgt gaaaatggtt gtttgtaaaa
    10021 ggaggctttt ttatggaaaa aattgccatt atcggtggcg ggatcattgg tatgactcta
    10081 gccagttact tagatacatc aaaattcaat gttgctttat ttgatgaagg gacaggacag
    10141 gcaactaaag ctagcgctgg gattatctcc ccttggttgt cgaaacgcag aaacaaacaa
    10201 tggtatcgtc tggctaaaga cggcgctgct tttttcccta aacttataaa agatttccaa
    10261 cttgatcaaa cgatttacgc acaatcaggg acacttttgc ttcgacctga aaaagattta
    10321 gctgaattgg ctgttcttgc cgagaaacga aaagaagaag cgccagaaat tggaaacatc
    10381 gaatggcttt cacctgaaca gacatctcaa gcacttccgt tattgaaacc agcagtttca
    10441 ctgcgtatca gtggtggtgg tcgtctggat ggaaaagcat atttagaaca tcttggtcag
    10501 cgtttagcac aaaaacaaat ggagcagatc caggcaaaag tcactttgca taagaagagc
    10561 gattcttggc tcatcaaggg accgtttgga gaatacacag ctgatcgtgt aattttgaca
    10621 cctggcccgg cacttaagcc tttattacaa gaattaggct atgctgcaga tatacgtccg
    10681 caaaaagggc agctcttagc atttcagaca tcttataaga tgagtcaaga ctggcctgtt
    10741 gctatgcttg acggagaagc tgatttcatt cctttcatgg atggaaagat ccttttaggt
    10801 gctacccatg aaaacgatca aggctgggat ttggctccta ctagcgaagc ttataaggga
    10861 ttgactaaga gtacagaaca gttcttgact gatccagatc agataaaaaa ctggccatac
    10921 gagttccgtg taggtacccg tgcttatacg tcagattttt ccccattttt cggcgtactg
    10981 ccagatgatc caagcttact ggctgctagc ggtctaggct cttctggctt gaccacaggt
    11041 ccttatatcg gctatctatt agccaacttc ttaaatactg gtcattggtc tacggaagat
    11101 tatcaaaagg acatctccac ctatattacg aaaaatccat catagaaaaa cagctttttc
    11161 aagtaaggat cccgagcgat attgcgcatg gattacttct tgaaaaagct gttattttag
    11221 ttcaatttct gaatcaaatt ctccaaactt tcttttgctt catctggcaa gagatctttt
    11281 cctatctgag cttcaatgaa tcgtaaacga atatcaagtc gttgtttcac cagattttta
    11341 taggacgtat cctcaaaaga aacaggaaaa tctggtatag gtaaatagtg gatgtcttca
    11401 aatggaccaa acgacttgaa ttctgccgtt tgttgcacga ttctttcaac gattgacaat
    11461 gtcgtttcag gtttgatttt tttatcatcg aaatatccag tattcaggac atagcaggct
    11521 gtttgctgtt tcaaaaacaa ctctcgaaaa gcttgatagt cttccctaag aggatagaca
    11581 cggaaaggat tagcaaatgg ttcaatgact agctcatcta gctgtctttt tgtagtgata
    11641 ttttccgcgt ctgatcgttt tgttgctaat gttgcaccaa aagacgctgc taaaactggg
    11701 tcattgatct tgataattgg cggaagcgaa tcatctttca tgatccaaaa aacagcatcg
    11761 atcttttcgc taagatgatc gactcggtcg ggtgtcgcat aacgtgattt gactgtccgc
    11821 ccatttccgt ttcgtatatc tcctgttact aggacttttt ctcctttact tgtacatgtc
    11881 acgcccacat tctgacaagt aaggaaagaa gaaatggccg gatcatcaga cggatagtct
    11941 tgtgttttat caaagtaaga aggttccaag gcagtagcaa aaccattttt tcgagaaata
    12001 acgaaagcat cgtcatgcaa gatcgaaaca gagtgacctt ccgtatgttt tgctaaggta
    12061 atcgttgatt ttccagaacc ggacaaccca aatacagcca tcgtataggt ctggtcatta
    12121 ctagcatatt gtttcatccc accgtggcag gcaataaagc cattgcgatt tcccaatgcc
    12181 caagcaagcg tcaaagtcgc tttttttaat tctccaaagt aacgtaatcc tagcacagcc
    12241 gctgtattat ggagagcatc aaacaaaatg agtccatttg gaaatgtttc atgtgtccat
    12301 tcgggatcac aaaagatata aagatctccc tcatcaaagg aaacagactt ttgataagtc
    12361 tctaagatct tcgaattcgc tggctgaaaa ttcagcaaat aagaatacaa attgttctca
    12421 aatccttctg gaatcagcaa atgactttgc acagaaaatt cttcagatag gccaacgatt
    12481 acttttgtat ggtaaaactg gcgcttgcga ctttgataca cagcttctct taaaattttc
    12541 tcgtatttgc tttgttcctt gtctaagtcc tcaaagatcc gccgagcact tgctgttctt
    12601 cccacaatgc ttccatcatt gaatactagt gtcctaacat cagctggtaa atctagttct
    12661 tcagcatgtg agattgttaa atctgtgatg atcgttcctg gactcccttt tgctagttga
    12721 taagctgtct tcctgtcaaa aacttcatga acattattac cgtaaaatgt cgtttctaca
    12781 atcgatcgaa tactggataa cagtggattt ccacttgtga tttcgtttaa ttcgagactc
    12841 tcttttgtac tcatggctcc tcctcattcc ctaaacaaaa aaacgttttc tttattatac
    12901 attattcgag tttttatttg tactgctgcg ctgtgggaag caaacgagtt atttttcaca
    12961 caaaaaaacc gagaacttga ataaacaagt ttctcggttt catcattttc cttttatcgg
    13021 cttagtacca gccgtttgct aaccagaatg ttttagcagc atcccatgaa ccataacgag
    13081 atgttacata ttgttctgct acacgttctt gattggcagc tgaatagtcg ccgtttaagt
    13141 atgaagcatc taattgataa cggccgatgt aacgtccgtt agtagctgtg tatgaaccgc
    13201 ctgattcttt ttgtgcgatc cattcttttg cagagcttga agttggcgct gttgcagcag
    13261 ctggggcagc aggagctact tcaactgttt caacaggtgc aacgtatgta tgttcttgcg
    13321 ttgctgtttc agttgtagat gctgtatttt ctgttgtttt gttttctgtt gttgctgaag
    13381 aatcatttga agttgggatt gttaattgtt caccaacata aatgcggttg atgtttgcga
    13441 tcgaattttt ttcagcaatt gcatcgatca ttgagttgtc tcctgcaaat ttttgtgaga
    13501 tcttagataa tgaatcacct gattttactg tataaacttc atcagcgtga gcatttgtag
    13561 ttcctacaaa cattgcagta gcaccagcag caaaagttgt acctaaaaca attttcttaa
    13621 atgaattcat attaaactcc tttttttctc cgttttttat tctttcgacg ctattaactt
//
LOCUS       DAYYCY010000068        13398 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000068 DAYYCY010000000
VERSION     DAYYCY010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.068"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(14..712)
                     /locus_tag="ACHAZB_001553"
                     /pseudo
     CDS             complement(14..712)
                     /locus_tag="ACHAZB_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
     gene            791..967
                     /locus_tag="ACHAZB_001554"
                     /pseudo
     CDS             791..967
                     /locus_tag="ACHAZB_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
     gene            complement(1143..1712)
                     /locus_tag="ACHAZB_001555"
     CDS             complement(1143..1712)
                     /locus_tag="ACHAZB_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFM8614230.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1786..2109)
                     /locus_tag="ACHAZB_001556"
     CDS             complement(1786..2109)
                     /locus_tag="ACHAZB_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8614231.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2263..3075)
                     /locus_tag="ACHAZB_001557"
     CDS             complement(2263..3075)
                     /locus_tag="ACHAZB_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8614232.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3402..4277
                     /locus_tag="ACHAZB_001558"
     CDS             3402..4277
                     /locus_tag="ACHAZB_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HFM8614233.1"
                     /translation="MSKRSTIQLMIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGT
                     FSRVQCTPDLLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLE
                     AMAENHATIDNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDD
                     ELLLLLDKFEKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAI
                     QLGISPRGSLSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKK
                     QIIHTLVAQIPIPVR"
     gene            4291..4614
                     /locus_tag="ACHAZB_001559"
     CDS             4291..4614
                     /locus_tag="ACHAZB_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8614234.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KNKRF"
     gene            4611..5363
                     /locus_tag="ACHAZB_001560"
     CDS             4611..5363
                     /locus_tag="ACHAZB_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HFM8614235.1"
                     /translation="MILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKR
                     EISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKL
                     SAHTQEWLYREYDYQEEECSPLFCFWGLPSPKFEPLLDLYFSLWHLRKEKQPELLILG
                     NRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKD
                     RTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5353..7524
                     /locus_tag="ACHAZB_001561"
                     /pseudo
     CDS             5353..7524
                     /locus_tag="ACHAZB_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(7636..9807)
                     /gene="copB"
                     /locus_tag="ACHAZB_001562"
     CDS             complement(7636..9807)
                     /gene="copB"
                     /locus_tag="ACHAZB_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HFM8614236.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIR"
     gene            complement(9827..12013)
                     /locus_tag="ACHAZB_001563"
     CDS             complement(9827..12013)
                     /locus_tag="ACHAZB_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM8614237.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEVKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLCLNRHMKK"
     gene            complement(12013..12222)
                     /gene="copZ"
                     /locus_tag="ACHAZB_001564"
     CDS             complement(12013..12222)
                     /gene="copZ"
                     /locus_tag="ACHAZB_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HFM8614238.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(12235..12675)
                     /locus_tag="ACHAZB_001565"
     CDS             complement(12235..12675)
                     /locus_tag="ACHAZB_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HFM8614239.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYYPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(12749..13288)
                     /locus_tag="ACHAZB_001566"
     CDS             complement(12749..13288)
                     /locus_tag="ACHAZB_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFM8614240.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQLFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT         3765 a         2807 c         2380 g         4446 t
ORIGIN      
        1 agggggggat tttttatcgt tgtggaatat ttaaatacca acgcttgtca tgaaaatctt
       61 gtgctccacc cttagtattt ccttctggat cgtttgtcgc acgcatcatg acgtatactt
      121 tcttattagg gaaatcacgc atattgaaag atacatgata gccaacattt cctaaagtat
      181 tataagcttg atttacgtct ggtctataaa tcccatcagc tcttactcta gctagttctt
      241 ttccagtatt gtagtccata atgaaaatat actcgtattt ataattagca atgtgccatc
      301 cagctacatg caagtttgcg ttttcgattt ctccgaactg atcaatgtgg gcgtaatttg
      361 ttccatctgt cagcgtagaa tttgcagcac ctgctcgagt tggatcaatt actggtttat
      421 cctccgaagt agttggatta tcatccgtaa aaccatgagc caaatcatag gctagttttt
      481 ctttacttac acccatttca gaaagataac cgtaaggatc tgtatgatcg ccccaaatat
      541 tttgtgttac ccataaatgc gatttgattc ctggttggtt ataaggagtg tctaatgtta
      601 atggaatacc atatttcatt gctgaatctc ttgccaattc aaagtatact ttatagttct
      661 tttcaaaagt ttctttatca tgtgtatgtt gtaactcgat ttgcacagga cttttggctc
      721 acgaggttgt cctactctac atcttgctga tagatatgct gaaaggaaaa caccaacatt
      781 atgttttgct ttttcagcta gaacgagaca agcaggaata cctttagaag atatcaaagg
      841 ttttttaggt cataaagatg tttccactac acaagtctat gcccatattt ctcccgaagt
      901 gaaaaaaaga tcaatgaatc aactagaaaa ctatatagaa gagcaaataa aaaagcactc
      961 aaattgagtg cttttttaag ctcactatca ctaaaactat cactaatttt atttataagc
     1021 gaacatgttt ctatcactga ttttttaaat ctagcgaaaa acccttatct taaaggcgtt
     1081 cgttcaattc tttggcaagg tcttcaaagc ctggcttgcc tagaagtgcg aacatggttt
     1141 tcttatagct catgtagaaa aattttcttg atgatgattt tatccatcaa ttgagattga
     1201 ctgttttgct ctcctatgat ttcagctatg attgtccgat tttgcggttt tcttcttaac
     1261 tggtagttga ttttcccagt ttccatcaac tcgctaagca tatccacgac tccgccaaat
     1321 ccggtctttt tcgtaataaa acctagtgct accattcgtt catctagatt ttttgctgta
     1381 atcgtcacac cagtaatgag cgagtgctgt aatgtttggg ccagctcttc catcccttca
     1441 gcattggata cgacggaagc tgttttgtta ataggttctt cgtttatttc ttttggttct
     1501 ttcctctcca ctttcactgt ttgacgaagg acatctagtt cttgcaagag cgtttcgtat
     1561 tgttcttggg tcagcatcac gccagctact ttttcacgat taaatatata tacgccagtt
     1621 cccgcgtctc gcgcttgagc aaatacatcc atcggcgatc gtttcacttc tgtgatcgaa
     1681 gatgtgggaa cttctaattt ctttaattcc aatggatttc ctcctttact aatcgctttt
     1741 tattatagca cagactgact tgttttgatc ttttttccaa aggttttatc ggtttttgcc
     1801 taaccagtaa ttgatatcgg taagaagtct ttcgatacca aatgaaggat ggtacagata
     1861 cgttttttga tcttcgttat ggtctagtgg aaaaaaatga tcgatgatca ctagatctgc
     1921 ataccgaggc ggcttatttc gagtacatac atgtatagag atccgttttc gatgtcttag
     1981 cgttttgttc agtaccccag caagtcttct tgcttcttgg tgttcatagc gaaaaaaaaa
     2041 gacgattttc acttcaggag ggtggacatc taaataccct cgttcctgga gtgttttcag
     2101 taaaagcatc ccccactcta ctgcggggtg tgcaagtgct tgttggatcg ctagccgaaa
     2161 ttgatatagt ttatctttgg aattgggaaa tttttctaat gtttcctgat aataacgaac
     2221 ttcttctatc agccggtcat ggataccaat tacgtaacga agtcattctt ggtaaagtac
     2281 ttttgtcagc tttgatttgc tttcgctctc aaattcgcta ttcagccaaa acagcgaatc
     2341 aagcttttca gtcacaatcg tcaatttccg atagtcttgt gtctcttcta tccgtttcca
     2401 ccgcttttca gtaatctggt tacagctcaa gcgcaaaatt gctagataat ttttttcttt
     2461 tattggcaaa atctgctcct ctctaaatag caattcagga aacaattcaa agaatagtgt
     2521 tcgactgtct cctaaaatct cgatttctgc cggatccttt ctttgcttca ataattggat
     2581 gaacatctca gaaaaggact gctctccatc atctgcaaaa aaatgcggaa aatctttcaa
     2641 aaagcaaaac agcagatatc ttacttttct ttctgaacca aagatctgtg cttttttata
     2701 agaaaaagaa aggtggtact cgccaaacca ctttctcaat cgctcttgcg tagaacgatc
     2761 gtacttctgc tcgtaaggtt cattcaatag cagatttttc agtaaacaaa acgggggtga
     2821 ttggatcaga aaaacaaaca agagataagg ttcgctatat ccttgatcat aaactaactg
     2881 aacggttttc ttatgaagaa tcagctgaat gggacagtcg ctttccgcca aacgtttcag
     2941 atgccgatcc actgttttaa catcgatttt cagttcttca gctaaatcag cttttcgcca
     3001 gatcaaatct ttcattagat tgattagtga aaataaattc tgtgcatttt ttgataatag
     3061 tttttgccat ctcatcgatc atttctcacc tgatttctaa acatattgtt tgatcaaaat
     3121 gggttcactc ctaaaaagat aaaaaactcc ctttctattt atttaaatac gcaaatcttc
     3181 ctcattattt tttattttac tcaatttaat cgtattctca tttcaaaacg aatgtacttt
     3241 aaaaaaacat aataagatca ctttgcttgt atgatgtatt cccttccatt acaatgaaat
     3301 aaatagcgat tttatgagaa ggtgatgtga aaatggaaga aaaaatagaa gaatccaaac
     3361 aaaaaatcga tcagttgata taagaattag aaaaagcggt catcagcaaa cgttcaacta
     3421 tccaattgat gatcgtggcc ctattagcag gtggtcatgt cttattcgaa gatattcccg
     3481 gtgtaggtaa aacgttactg gtcaaagcac tagcaaaagc tgtacaggga accttcagca
     3541 gagtccaatg tactcctgat ctgctgccta gtgatattct tggtttttcc gtatacaatt
     3601 cacaatcaaa agagtttgaa tttcgtccgg gaccgatctt taccacgatc ttgttagcag
     3661 atgagatcaa tcgtacgact cctcgtactc agtcagcact tttagaagca atggccgaga
     3721 atcatgcaac gatcgacaac aatacatacc ctctagacaa tcattttttt gtcttagcta
     3781 cgcaaaatcc aatcgagtac gaaggaacat atcctttgcc ggaagcacag ctcgatcgtt
     3841 ttttattccg actacaaatc ggttatcctt cttttgatga cgaacttttg ctacttttgg
     3901 ataaattcga aaaagtgttg gataacttaa atgaagtatt gaacagttat gaaatagaag
     3961 aaataaaaac aaatgtggcg aatgtctttg tcagtcccga agtggcatct tatgccttgc
     4021 agttagtgtc agcaacaaga agtcatgctg ccatccagct tggcatcagt ccacgaggaa
     4081 gcctttcctt tattcaggca gcaaaagctt atgctttaat ccatgggaga aattacgtca
     4141 cgcccaaaga tttgcaggat ttagttcctt acgtgttttc ccatcgcctg attttttacg
     4201 atcgttcatt gaaggaagac gaaaagaaac aaatcattca tactcttgtt gcacaaattc
     4261 ctattcctgt gaggtagatt gaggtagaca atggaaaaaa tcaaagatta tttcattaat
     4321 tttgtgttat ttctggtata tttcatcatt gtgctttttg ctcttgtatt caacaattct
     4381 ttaggctggt tcttgttggc cttttttact ctttatgtat catggagctt gatttcatta
     4441 agcagctttc taaagaaact ttcggttact tgtacattgc agccactcgt ttatcgcaaa
     4501 aaaagtatgc aggttatttt ttccatagca aaaaagagaa ctttttcttt acctctccct
     4561 cgtttacaaa tcggcttccc agcaatcttt gcagaaaaaa acaagagatt ttgattctta
     4621 caaataaacc gcaagaagtc caattgcttt ggcagccaaa agaaagagga tgccatgaaa
     4681 cattaactgt catttttaca gcctatgata gtctaggact tttcagaaaa agtgcaaaaa
     4741 gagagatctc ttttcctgtg actgttctgc ctgctttttg taagattcaa gcagaaaaat
     4801 tacagagagt tctggaaaaa aaacagcagc tgaacgcgta ccaaagaggt cttgattttc
     4861 gggaacatcg cgcttatcgt ccaggtgatt ctttcaaccg aatcgattgg aaattgtctg
     4921 ctcatactca agagtggtta taccgtgaat acgattatca agaagaagaa tgttctcctc
     4981 ttttttgctt ttggggtttg cccagtccaa aatttgaacc tctgctagac ttgtattttt
     5041 ctttatggca tttgagaaaa gaaaagcagc cggaacttct gatattaggc aaccgcgcct
     5101 ttcatggcaa agaccccggt catacctatt ttgcttcttt ggaagctggc gatgagaaag
     5161 cttttttcgc acacttgaaa aaatatgcaa aaaaacaaat cgtcctcttt gttcctgaga
     5221 attctcctga agtctcagaa gctattgaaa cattgtcaaa agatcgaacg gtttatcttg
     5281 tttatttcgt agaaaaacaa ctcatggtcc aagaaaaaga caagatatgt tcattgccag
     5341 gaggtgaatg gattgacgat taaacgggaa aaatggctgc gtggcgttta ctcctttgtg
     5401 atactaagtg taggtgctcc gatatttgta gtagctaatc aattgcaaaa cagctacctt
     5461 cttatcctaa ctattggtgt cctatgcctt attgtctgct gtattcctaa aaaatgggta
     5521 cgtttgctgc tttctttgcc agtaatgata ggttgtttgt ttttatattt tccttcgaag
     5581 acattttcca tattatggat cttacagttt ttttcgtcag tcacagagga attcttccat
     5641 ctatcccaag gtaaacttta ttatttgcca gaaaagacag ctttttttct cataatgttg
     5701 ctgatctatt tatttattac attgaccgtg gattacgact attggtacgc accctttctt
     5761 tctttgatgg cttacttcat gatgcttacc gtttttcgga aaaaagattt actgtacgaa
     5821 atgattgcgg tagtaactgt tttgttcgtg tatctggcag caaggcaatt tttctccatc
     5881 cggttgttgt cgggaaacac tcgattccag tcattgacta cagttctgct tatttgtttc
     5941 ttaactttta gcgctatctt gcctttatat cttccgaaag tacaccaatc tctcgaggaa
     6001 acgagtgaac cgatccgaga atatcttaat gatgaagggc tttatgacac actccatgag
     6061 tatcaattaa ccggaagtgc aagaacaggc ttcagtgaga atgatgaaca gcttggtgga
     6121 cctttatatg ataatggcga agttgttttt acagcgaacc aaaaaggaaa tggctattgg
     6181 aagatcgaaa ataaaaatca ttacaccggt tctggatgga tagaagatca ggacgcaccc
     6241 atccaaccaa tccaagaatt ccctgttgac atcacgagct accaaaccgt tatagaagac
     6301 aaagaaaaca ttacattgac cctaaaaaaa caagttttct taccttatcc ttatggaaat
     6361 actaccttga ctgatttttc attagctagt ggacaaacgc tttactattt gcctgaaagc
     6421 cagcgattca ttcctgtccc gaatgaatcg acacgttctg caactttaca aacagaaagg
     6481 ttagttttta cgagagaaga attaaaaagc agtcagttaa aagctgatga tttagaaaat
     6541 cagcagacaa atcttcaatt gccaaaaagt ttgcctaaac gtattgagga cttggcggtc
     6601 gaattgacca aaaatgaagc aactttaatt cgtaaagtcg aagctgtcta acagtattta
     6661 aaaacaagcg gaaatttccg ctattccaaa acggataccg ggcatactcc aaaagaacac
     6721 gattatgtgg attatttcct ctttgattca aaagtcggct attgtgacaa cttttcaact
     6781 tctatggtcg tccttcttcg aacacttggt atcccagctc gctggacaaa aggttttaca
     6841 aatgggctca gaacaggcac agataatcaa gggaatgagg aatacagcat tctcaatagt
     6901 catgctcatt cttggccaga ggtatacttt cctggatttg gctggcttcc atttgaacca
     6961 accccttcat ttgccaatcc aatgggttca caaccagttg ctgaaacaac cgaaaccgtc
     7021 cagcctgcta tcgaacaacc aaatgaaagc gaaaatatgg cagaaactat ttccagtgag
     7081 agatctgagg tcattccatc agaagaaaca acactcacac cagcttataa aatcgtctta
     7141 ttcagccttg gagtgtgttt atccgtcttg ttgttctttc tattgttatt cagaaaatct
     7201 ctgcgctgga caagttatct tatctatctt catttttttg cttctactaa tacgattgca
     7261 tatcaaaaac ttcttaaccc tttggaaaca ttggtaccgc gcaaacccgc agaatctttg
     7321 caggaatata gcctgcgaat agaacaagaa ttcaagctag atctgcgttt cattcgatta
     7381 actactcaat atgaagcaga aatatatgga cgacaagcag atccggtaaa attcgataaa
     7441 aaaacctatt tagaaataac gcatataata agaaaaatgg tatacaaaaa aattctgaca
     7501 aaagggagcc gcttcaaaaa ataagataaa atttctggag aatctacttc tggaatgccg
     7561 agaaccagct gattgagtat cgattactcg aaaaaaaggt tgtgacacgt tttttgtcac
     7621 aacctttttt cattattatc tgattttcaa tgtcaaagca tttagagcta caactaccgt
     7681 acttaacgac atcaagacag caccaactgc tggacttaat atgataccaa ttggcgcaag
     7741 aattccggct gctagcggaa ttgcaagaat attgtaacct gccccccacc agaggttctg
     7801 gatcattttc tttcgcgttt gcttggctag atcaagaaaa tgcaaaatat cttttggatc
     7861 gctgtttgtc aatacgacat ccgcagaatc gattgcgata tctgtccctg caccgattgc
     7921 catcccgatt gaagcacggg ctaaacttgg cgcatcattg atcccatcac ccaccatgat
     7981 tacttttttc ccttggtcaa ggtatttctg gacaacagct tctttatcat ctggtaataa
     8041 accgccataa tattcttcga tccctaaata atctgctact gcttgggcag cgtttttatt
     8101 gtcaccagta agcatgacag gagtgatttt tctttctttc aatgttcgga taaattcttt
     8161 tgcttctgtt tttactacgt cacctaaagc aacgattgca gctagatgat tttctaaaat
     8221 caggaagctg atggtatttc cttgttcttg gtaaggtttc aataatgctt gttctacgtt
     8281 taatccgaga cgctcaactt ctttttcatt aactattttc acttgttgtt ttttgactgt
     8341 tgcctctagc ccgactcctg aaagattttt tagattttca gcttgataag gcttgatttc
     8401 atgttctttc aaataattca taatgccaac agccaatgga tgattggcat tcgcttccaa
     8461 ggctcctaga tattgaagga tttcattttt attgaacgca tcatcaagta cttcgacacc
     8521 cgttacagta aattttcctt ctgttagtgt tcctgttttg tccagcatga tcacatctag
     8581 atcattggct tgctccagtg cattgcggtt tttcaaaagt aatccgtttt tagctgctat
     8641 tgatgtggaa cgagcaacaa ctaatgggat agctaaacct agagcatgag gacaggcgat
     8701 gataaagact gttaccatac gttctaatgc attaggaaga tctgtcaaga acaaccaggc
     8761 gatgaatgcc aggataccca caattaatgc gacataaaac agccattttg ccactttgtc
     8821 agataatgac tctagcttgg atttttctcc ttgtgctttt cgcaccattt ccataacctt
     8881 cgctagataa ccattttcac cagttccagt tacttcgatt tcgatcgttc catccccatt
     8941 gatagaaccg ccaatcactt tgtcattggc ttgttttttg attcctcttg attcgccagt
     9001 cacagctgat tcgtcaacaa ttgtttcacc tttcaagatt acaccatctg ttggcatttt
     9061 atcaccagac cggacgatca aacgatcacc ttcgtttact tctttcaatg aaactgtctc
     9121 ttcttttcca tctttcgtca agcgtttcac tgattctggc agtaattctg ctagtttttg
     9181 taaagcattg ctggcattcg acacagcatt catttcaatc cagtgaccta gtagcataat
     9241 aacgatcaaa gtagccaatt cccagaagaa atccatgacg tgtgtatgtg gattgagcag
     9301 atttgcgata aacgaataga cactataaat atatgcaact gtaatcccca tcgcgatcag
     9361 tgtcatcata gcaggacttt tttgtttcaa ttccatctta gctccactta aaaatggctg
     9421 accgccatag ataaatagta tagttgctaa gatcaatacg agccaatcag aaccaggaaa
     9481 cgttacttga aatggaaatt ccattcccat cattggtgaa aaaagaatga ttgggatagc
     9541 taggataagc gatagccaaa atttttgttt gaaattcccc atgtgcatgg aatgatccat
     9601 gccgccgtgt tcgtgatcca tgtgcatcga atggtccatt tgtttgctgt ttgctatatc
     9661 tccatggtta tgtcccatgt gcatggaatg atccatctga ctatggtcat gtttcatctc
     9721 catctgtttt tcatgagatt ctgtactttc tttgtttact acttcttttt tatgctcatg
     9781 ttttttctcc atatcgttcc cattcatttt tactccatcc cttctcttac tttttcatgt
     9841 gtcgatttaa gcacagggag ttcaacaata cactgacgga actaaacgcc atcgctcctc
     9901 ctgcaatgat tggattcaaa aagcctagtg cggcaaaagg aataccaatc gtattataga
     9961 caaaagccca gaaaagattt tgtttgattt ttctcaatgt caacctagac aactcgattg
    10021 tttgtgcaat ggatgccaaa ctgctattca tcagcgtcac atcggctgtt tccatcgcaa
    10081 tgtccgttcc acttcccatt gccataccga catcagctaa tgctaaggcc ggtgcatcat
    10141 tgattccatc gcctgccata ccgacttttt tgccatcttt ctgaagtttt tctacgtagg
    10201 ctgctttatc ctctggaagt acttcagcaa agatatgctt tggatcaata ccaacttgtt
    10261 taccaatcgt ttcggctgcc agtttgttgt ctcctgtcag cataaaaaca tctaatcctt
    10321 tattctggag ctgcttgatt gcttgtttga cttctagttt gatttgatca gccacggcaa
    10381 tcaacccgat cacttgcttt tcatcagcta aaaacatgac tgtttttccc tgctgttcta
    10441 attctaacgc gtgttcttgg tattcatcaa atgataggtt caattcaatt aatcgtttac
    10501 gtgtacccgc aaaatagcgt actccgttaa tcgtaccgct gattccagca cctgggtggg
    10561 ctgtaaaatc aatcatatcg tatgttgctg cttcttgcag ttttccgtat tccacgattg
    10621 ctttacctag tggatgttca gaagcttgtt ctaaagtata aaaaatagat aggacttctt
    10681 tagaaccgac tagatctgta actttgggct ttccttctgt aatcgttcca gttttatcca
    10741 aaacaatgct gtctaagtga gcagctgctt ctaatgcttc tccgccttta attaaaattc
    10801 catttcgtgc accaacaccc gttcctacca taatagctgt tggagtcgct aaacctaatg
    10861 cacatggaca cgcaatgact aatacagaga cactatggat caatgcgagt tgccaatcac
    10921 cagtgatcaa acctgtagct atgagcgtga taaacgcgat gactaatacg atcggaacaa
    10981 aaatccctga gatccgatct gcgatttttt ggattggggc tttgctccct tgagcatctt
    11041 ccaccatttg aatgatttgc gctaatactg tctgcttgcc gacttgtgta acttccatgt
    11101 gaagtagacc attcgtattg attgtcccgc caaagagcgt gtcatccgga ttcttttcta
    11161 caggaagatt ttcaccagta agcatacttt catctatcgc actgcttcct gaaataattc
    11221 gaccatctgc tggaatctgc tcaccaggac ggatgactag ttgatcacca acgactactt
    11281 cttcaatcgc taacgtttct tctttcccgt ttcttatcac ttgggcggtt tttgtttgaa
    11341 gagacatcaa ttgtttgatt gcattacctg ttttcgtttt tgctgtatgt tccaaatatt
    11401 tgccgagcaa aatcagcgtg atgatcatac tgctgctttc aaaataaaga tcttgggggt
    11461 gtccacggaa aaatccgttg taaatgctca atgcaaaggc agccgatgtt ccaattgcta
    11521 ccaatacatc catatttggt gctttcgtct tgattgcatg gtacgcacct ttataaaaac
    11581 gagcaccgac ataaaattgg acaggagcag acagcactaa ctggacgatt ggaaaatgaa
    11641 aaaagtgaac aatcgccgca tggcttccta acatcatagc aatcattgac aacatcaacg
    11701 gcaaagtcaa aaccgtactc aatataagat cgaatagcat ttttttcaaa tacgcttgct
    11761 tttcttctgc aattttttgt ttgtgggctt catcgtacaa aattgcacca tagccgatgt
    11821 tttctacgct ttggattaat ttttcagtgg tcgtcccttc atatttgaca cttgcttttt
    11881 cagtggccaa gttgactgtg gcattgacca caccaggttg tccgttcagc tctttttcga
    11941 tcctagcaga acaatttgca caagtcatac ctgtaatgac gaacgtatcc attttggttt
    12001 ctgctgttgc catcaaatca cctctgcttt ataacccaat ccattgattg cttgacaaat
    12061 cttttcggat tgaacttttg tttcatcaaa cttgaccgta ccgtttgctt ttttcaaatt
    12121 tactttcact ttttgcacgc cgtcaagtgc gcttactgtt tcttctactc ttgctgcaca
    12181 atgattgcag ctcatacctt cgattgaaaa ttgttgtttc attttttctt cctcctattg
    12241 atgttgttta cattcacatt gacctgggat acagttacat tcgatcgtat ctactggttg
    12301 cttttgttcc agttgtttca aaatctcatt gatatcttct tgagtcaaag tggcttcttc
    12361 tactagatcc gcaatcgtct ctccaatcct ttttgcacag atatgggaaa aaagattttc
    12421 cgtcgcactg cgaactgttt ccatctccga gacagctgga taataaatga atttttttcc
    12481 ttcttgttct gtccagatca cttctttttt taccaaacga cccagcaaag ttttgatcgt
    12541 tgctggtttc caatccatcg aatcagctaa gatctgcgtg atttcttggg ctgtcgtctg
    12601 tccaagtgtc caaataacac gcatgatctc ccactctgaa tcactgatct ttactggcgt
    12661 tttgactttt tccatttatt tcacaccttt cgtttacaaa tgtaatcatt atcttatgaa
    12721 tacagattac acttgtaaac aaaaactgtc aattattttt gctctctatt ttcaatttag
    12781 ttaaaaattc ttgtttctct cgtcttgttt taaggatgat cagtttattt cttgcattag
    12841 ctacttggat cagctgctgt attttcggtt cattgttttc ttcaaaatgc cagataaaag
    12901 ataaaaattc taagtattcc ctgtttagtt tctccgaaaa tgattcaggc atatctggcc
    12961 gagatttttt gtttatcttt ctttttagag aacgccaaac tactcggtag atggcgagat
    13021 aacgcggcac tttaagccaa atgatcttat ctgcttcttt aatcctttcc tctaatgtag
    13081 aagtataatt tccttcaacg atccatgacg gattagatac cataaacaat tgctgttctt
    13141 gcaaaaacca cttttttgcc tgaatactgt agtctgttgt atgccataaa aggtctagcg
    13201 gcagtagcgg aaagtcggtg atttctttga tcttccttgc gagggtcgat tttcccgacc
    13261 cactttgtcc aatcacgatg atcttcattc ttctccctca cttctctttg gaatttggtt
    13321 cgatcatacc acttataacg ccgcaaaaaa acaagggact gtgacaaaag tcgtgtcaaa
    13381 gtcccttgtt tcgaataa
//
LOCUS       DAYYCY010000069        13367 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000069 DAYYCY010000000
VERSION     DAYYCY010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13367
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.069"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            182..949
                     /locus_tag="ACHAZB_001567"
     CDS             182..949
                     /locus_tag="ACHAZB_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8614241.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            939..2981
                     /locus_tag="ACHAZB_001568"
     CDS             939..2981
                     /locus_tag="ACHAZB_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8614242.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            3262..3411
                     /locus_tag="ACHAZB_001569"
     CDS             3262..3411
                     /locus_tag="ACHAZB_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HFM8614243.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            3422..4936
                     /gene="dltA"
                     /locus_tag="ACHAZB_001570"
     CDS             3422..4936
                     /gene="dltA"
                     /locus_tag="ACHAZB_001570"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047473 - D-alanine [D-alanyl carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HFM8614244.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            4933..6138
                     /gene="dltB"
                     /locus_tag="ACHAZB_001571"
     CDS             4933..6138
                     /gene="dltB"
                     /locus_tag="ACHAZB_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HFM8614245.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            6183..6416
                     /gene="dltC"
                     /locus_tag="ACHAZB_001572"
     CDS             6183..6416
                     /gene="dltC"
                     /locus_tag="ACHAZB_001572"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036370 - D-alanyl carrier activity
                     [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HFM8614246.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            6419..7687
                     /gene="dltD"
                     /locus_tag="ACHAZB_001573"
     CDS             6419..7687
                     /gene="dltD"
                     /locus_tag="ACHAZB_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070394 - lipoteichoic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HFM8614247.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            7828..8910
                     /locus_tag="ACHAZB_001574"
     CDS             7828..8910
                     /locus_tag="ACHAZB_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HFM8614248.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            8910..9713
                     /locus_tag="ACHAZB_001575"
     CDS             8910..9713
                     /locus_tag="ACHAZB_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFM8614249.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            9783..10523
                     /locus_tag="ACHAZB_001576"
     CDS             9783..10523
                     /locus_tag="ACHAZB_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFM8614250.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            10534..11538
                     /locus_tag="ACHAZB_001577"
     CDS             10534..11538
                     /locus_tag="ACHAZB_001577"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HFM8614251.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(11583..>13367)
                     /locus_tag="ACHAZB_001578"
     CDS             complement(11583..>13367)
                     /locus_tag="ACHAZB_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8614252.1"
                     /translation="SIIIILSLLFLTGIAYFFYFIFSPADSNQMKEIKDSWVFYTKDD
                     PDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLLIKGNHQWITVYLGEKILYQR
                     KDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNYAGLPPKVYLGTTGPLISFIY
                     SQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSLILLSCFALALGFEAVSEDIL
                     SGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKKWYGIFCTFQLLIFIGVLLYA
                     TFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNRFYVACTPWIVLVAIIHCLIY
                     IQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNTDKDRRQVDFLKIKNDLLEQH
                     YEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKNYVEKILEEAKNFEMLCSFCN
                     HQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDDLAQLLIHALEHSFRETHAIE
                     NPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGITEELDDQERFDLMMMKDVADR
                     YTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT         4297 a         2191 c         2702 g         4177 t
ORIGIN      
        1 aaggtgaaaa cttgtagctg gtggatttgt tttttgcata tttaattttg ctgaataaaa
       61 taattgaata aaatttttta ttcagtccaa gtaaaagtga caaaaacata agagcaaatt
      121 attcgtagat atgctactat gcaaataaat agaaatgaaa aatgaaagag gaggagaaga
      181 attggaaaag atgatcgaag tgaaacatct aagtaaaagc tacggcagca aactttctcc
      241 cgtcaaagcc ttagataata tctcattcac agtagaagaa ggcgagtttg ttgggattat
      301 gggcccaagt ggtgcaggaa agacaacgct gatgaatatc ttgtccacga tcaatttgcc
      361 tacgatgggc agtgtctcga tccaaggaaa agaaatcaca aaaatgaaaa acagtgagct
      421 cagtgacttt cgtcgaaaaa aactaggatt catttttcaa gagttcaatt tgatcgatac
      481 cctaaatgca aaagacaata tcctattgcc cctagcagtt gaacggatga ctaaagagga
      541 aatggaaaag agagtaagac atgtagctca attgttgaat atagaagaac tgctcaaacg
      601 gtatcccgat gaactttctg tcggacagag acaacgaatt gcagctgcta gagcacttgt
      661 tgttcagcca caagtgattt ttgcagatga accaactggc tctttggact cgaagtcggc
      721 tacagaattg ttgaactact tgaagaccat gaatcaaaaa gaaaaagcaa cgatactgtt
      781 agtcacccat gatccatata cagcaagcta ctgcgataga attttattta ttaaagatgg
      841 tgtgattttt tctgaagttg tcagaagagg gacaagaaga gagttctttg agaaagtaat
      901 agacatgcag gcgacgattg gcggaggagg aaaaatgaat gctgtataaa ctagccgtta
      961 aaagtttttt tcggcagtca cgaggctatc ttgtttattt tttcagtttg actttatccg
     1021 taatgatcta ttattccttc agcgcaatga cctacgatca atctttagtc agaagagcaa
     1081 gtcaagatac ttcgatagat ggtggattga gttttggtgg atttatcatt actgttgttt
     1141 tgcttttttt cgtattcagt gccaaccgat ttttcttgaa tagaaggcaa aaggaaatag
     1201 gcatctatca gctttttggt atgaataaac tgcagatatc aggtatatat gtgttagaaa
     1261 tcatgataat tggattgttc gcctgtattt ctgggatatt actcggtatt attttttcca
     1321 aactcttttc aatgattttg gttcgaatga tggatatgga tcttactagt ccatttttta
     1381 tctccattcc atcagtagta gatactttat ttgctttttt catcatcctt ttagcagtat
     1441 ctgtatattc aatatggaaa atatggcgct atccaatgat tcgaagtttt ggagaaaaag
     1501 aacaagcaga tagttcaaca atgcgcttca ggacaaggca ccgattactc gcagtcatcg
     1561 ggttactact tttaacgagc ggatattttg gagctagtca ttttagggaa atcacttcat
     1621 ggatgatcag ttatcattat cttgatacat tagttttctt ttttcctttt ttaatttttt
     1681 tcgtttgtgt ggtcggtact tatttgtttt tctgttatac atttcggtta tttttgatca
     1741 tattgagtaa atggaagatt tattactatg gtatcaattt tttgataatg ggtaacacgc
     1801 aagttcattt gctcaaaagt tggcgtacaa attctctgat tacagtcatt ctcggaattt
     1861 ctttaatgac aattggcggc acggctagta tctctactat tttatcctac caggagaaag
     1921 tttcctcccc gatggactat cagtttgatg ttgagacaga gaaaaaaatc aagccgattc
     1981 tagcagagga agggcagaag ataactaaag aaatcacctt gcattataaa gtcattggga
     2041 gtatctatag tcaaaatctc gaagaaccta tgtttcaaac tgctaatctg atcacggaaa
     2101 cacaatatca agccttcaga aaagttaatc cagatttgcc gaacatttct ttgaaatcac
     2161 ctggtcatac tgttttgctt gatcaaatgc aaactctttt tcggaatatt tcattatatg
     2221 gatctagtat tggactgcca gataatcaag aattgaagat tcaacaaatg cttccaagtg
     2281 tattaggtga cgaaacaatg gtatatctaa gtcctgtact gattgtagat gataccgttt
     2341 ttgaaaaagc aaaaggtgtg gattatcaag tagtcaatgt agatgtatca ggtggaaata
     2401 atgaaaaaat agatgaacga ctgagcaatg acctgtcgat aaagtggctc aatgctgttt
     2461 attattctta tgatatcgta aatggaagtc tacaaggaga aattagcaca aagaaacttc
     2521 cggatgacca aatgaaggaa gcacaattta gccaagaaag ctctcgtttg aatttctctt
     2581 ccagctattc gaaattgcgt gcagttaatc ggcgaatcgg tatcaatatt tttgtcacac
     2641 tatttgtcgg gatgatcgtg attattgcta ctggtagtat tttgacagtc cggcaattag
     2701 cagaagcaga ggaagaaaaa gaaaattatc acctattgac taagctgggg attccacaaa
     2761 gaaggatcag acggatgatc ttcgagcaaa acgcacttac cttctttcca cccatgatat
     2821 tagggatcac tcacgcagta tttgcaatca atgtcttcac tcagtatata aaaactgccg
     2881 attactggtt ggcttatctt gtgagtggct tattgatcct tatctatctt ttgctttatg
     2941 tcattacgag tcagttatat tgtcggatta tcgaggaatg atatacggaa taagacaaga
     3001 taataaatag aactaaattt tgaataccat ttatttggaa taagaggttg tgacacgatt
     3061 tttgtcacaa cctctttatt ttttatattt tttaaaagta cgagaaatta taaagaaaaa
     3121 agcagtgatt tgatgtgcta tttttataaa agctatgtga caaaactgtt agtaaaaaaa
     3181 cggtttctgt aagctagaca catgtttctt tttgtcaaaa ttagttacac tttgtgtgag
     3241 gttatatgag gagggaatcc aatgaacaaa tggcgttcta atccaaaatt gaattattgg
     3301 ggaaaattca tcattcgaac aattttttat agtgcagtac tgctgttcct gatttatctg
     3361 tatcactata aaaatattca aggaggaact tttatctata acgaatttta ggaggaagag
     3421 tatggaaatc aaaacgatta ttgaagcaat cgatgattgg ggaataaatg aacctgatcg
     3481 agttgcttat caggcggatg agacgcatac ttttggtgaa ttgaaacaag catcagacgc
     3541 acttgcttat tatttagaaa aaaaggtaga aggcgatgga ccaattgtcg tttttggcaa
     3601 tttggagttt gaaatgattg tctcatttct aggggttgtc aaatctggcc atgcctattt
     3661 gccgattgaa gagcatacac ccaaggagcg tatcctatcg atctttcggg tggctaaacc
     3721 atctatggtc atcagtatcg gggactggat agaagatatt ccttcggttc ctgtcataac
     3781 aaaaaaagaa tttcaagaaa tcaagaagat accagtagga ttttatccca gaaatagcgt
     3841 aaaaggtaca gaaaattttt atattatttt tacttctggt actactggag aaccaaaagg
     3901 cgtacaaatc agtcatgata atctagtcag ctttgttcaa tggacgatgg aagactttgg
     3961 gatcaagcct ggtatgcatt tcttagccca agcgccattt tcttttgatt tatcggtatt
     4021 ttctatttat cctgcattag tttctggcgg aatgctgaag ccgttaaata aagcagttgt
     4081 tcaagacttt cgtcaattat tcgcgacatt gccagccttg aagttgaatg tgtgggtttc
     4141 tactccatca tttatggata tttgcttgat ggaacctaca ttcaatggag aaaatgtgcc
     4201 ggaattggac atgtttttat tttgcggaga agaattgacg aaaaaaactg cagaaagtct
     4261 acttgatcga ttcccaaatg cacggatata taatacttac ggtccaacag aagcaactgt
     4321 agctatttca agtattcaga ttgaccagca tgtactagat agttatgacc gactaccgat
     4381 tggctacgta aaaaaagaca cgaaagtttc tattgtccaa gaaggtaaac ctgtttcaaa
     4441 aggagaaacg ggtgaaatca ttattgcagg accaagcgta tcaaaaggtt acctgaacaa
     4501 tcccgaaaag acagctgctg cgttcttttg tcaaaatgac ttagcaagtt accatacagg
     4561 ggatgcaggt agactagatg aatatgggct tttattttat gaaggacgta tggatcagca
     4621 ggtcaaatta catggatacc ggattgaact cggggatatc gaacattatc tgctccaaga
     4681 caaccgaatc aaacaagctg tcgtcgtacc aaaataccaa ggaacaaaag tacagcagct
     4741 ggttgctttt gtcgtattag aaaacaaaac gaaacagccg gattttcagt tgactaaatc
     4801 tatcaaagaa caattactag aagttgtaat ggattatatg attcctcaga agttccatta
     4861 tgtcgatctg ctgcctcaga cagttaatgg aaaaatcgat cgaaaaaaat taacagctga
     4921 ggtgaatcct gaatgatgtt acctcatatg atcccttatg cagagccgtt ttattttttt
     4981 cttttagcca ttgcgcttgt accgatcgtt ctatctttat tgatatggga aaaaagatta
     5041 ccagtttatc aatcagcagt gacacttttc tttttatttg tcagctttgg aggtaattat
     5101 tggcaacagg gagcggcgct gattgggtat gttttgtggc agacgatact tgtatggggc
     5161 tattttgttt atcggcaaag gaaaaatcag acaggatggt tttttgccgc cgttatttta
     5221 gcgataattc cactgttttt aacgaaagtg actccttttt ttgaacatgg aaaagcatcg
     5281 ttacttggtt ttttaggaat ctcttattta acctttaaat ctgtacaaat gatcatggaa
     5341 ataagagatg gcatgatcaa aacatataag ccatttcgtt atatccagtt tctgctgttc
     5401 tttccaacaa tctcttctgg accaatcgat cgctatagga gatttgaaaa agatcagctc
     5461 catccgcctg ttcgtgataa atatgttgat cttttgggaa aaggcgtcca aaatattttt
     5521 gtcggttttt tatataaatt cattatcggt tattattttg gtcaagtact gcttcctgta
     5581 gtgggacgca ttgccttaca gcatggtggt atttcatggg cattagtagg ttatatgtac
     5641 gtgtacagta tgtatttgtt ttttgatttt gctggctata gcttatttgc agtcggtaca
     5701 agctacatga tgggatatca gacaccaatc aatttcaata aacctttttt atcatggaat
     5761 atcaaagaat tctggaatcg ttggcatatg acgctgtcct tttggttccg tgattatgta
     5821 tatatgcgat tgatgttctt catgatgaag aaaaaatttt ttaaaagcaa gatcgtaatg
     5881 tcaaatatcg gttattttgc tttgtttctt ctaatgggta tatggcatgg attgacgtgg
     5941 tactacatcg tatatggact gtatcatgct tgtctgatct gtctgacaga tgcatggatc
     6001 cgatttaaga agaagcataa agagcagttg ccttctaaca aatttacgca tggcttagcc
     6061 gtatttttga catttcaagc agtatgtttt agcttcctga ttttttcagg atttttagat
     6121 aaactttttt ttagctaacg aatagtgaca tattaattta tataacaaag gagaaaagga
     6181 aaatggaaga acaaatttta tcaatcttag aagaaataac aggaacagat gaagtaagaa
     6241 aaaatcgtga tattgatcta tttgatgaag ggttgcttga ttctttagca tctgttcaat
     6301 tattagtcga gctagacgga gagctggata tccaagtccc tgtatctgaa ttcgagcgag
     6361 aggattggag cacaccgaat aaaatcattc aacaagcaac tgcattgaag ggttgagtat
     6421 gaaaaagaaa ctttttgcta ttttcggccc ggttgtttta gctgttggct tactgtttgt
     6481 gttttttacc tcctcctatc aagtagatat ctctaatcca gaaacgatta gacgtgcctc
     6541 ttcttccatg tcagtgaacg tgttgaaagg agatgcgata aaaaattatg cattatctga
     6601 aaaacaatat attccttttt ttggctcaag tgaattaagc agaatcagtc catttcatcc
     6661 gtctgttctg gcagaaaaat accagcgaaa ctatcgtcca tttttgctgg gagcacccgg
     6721 gacacaatcg ttaagccaat atatgatgat gcgttctgca ggagatgcta tgaaaaacaa
     6781 gaaagtcgtc tttattattt ctcctcagtg gttcgttaaa aatggtgtaa aaactgatta
     6841 ctttaatacg tattattctg aacttcaaac atacgactgg ttgttttcaa tgaagaaagt
     6901 aacgcctgct gatcgctatt tagctagaag attattaact ttctcaaaag taaaagagaa
     6961 tgatacgttg acggcgattc tgcaaacgat caaaaaaggg aagctacctt tacctgaatc
     7021 tttgaatcag ctgcgcagcc aatggaacat gctgaagaga gaggatgaag tattcagcaa
     7081 tattgggttg aaagatcgac agcaaaaaat cgatcatgag tcaaaacgat taccaaaaca
     7141 ataccaagaa acagaattat ccattttagc aaatcaaatt ggagaacgag aaacgacgaa
     7201 taatccattc gggttgaaaa acgattttta tacgcatcgt atccgagccc acgagccaga
     7261 gctcaaacag tcacaaaaaa attgggatta tcgtttttcg ccagaatttt ctgattttca
     7321 gctggtcttg gatcaattag caaaaaacca taacgaagta ttgttcatca tcccaccggt
     7381 taacgagaaa tggagcgatt ataccggact atctcaagaa atgttacaag gatttgccaa
     7441 aaagatcaaa tttcaattaa atagccaagg attcaatcga atagctgatt ttgtcaatca
     7501 agcgggtacc aactatttta tggaagacac gatccatcta ggctggaaag gctggttggc
     7561 tgcagatcaa cagatcagac cgtttttaga agaaaatcat attactgcta gtaagtatca
     7621 tcttgacgat gcttttttta gcaaatcttg gcaacatcaa ataccagata aactacaatt
     7681 aaaataaaga aaacaggcga gtgttgctcg cttgttttct ttatttttga atattctgaa
     7741 taaaagataa aaataccaag tttgcctgaa caaaaatcat aagcaatggt ataatcacaa
     7801 tatgaatata gaaatggggg aaaacatttg gatacaaaag aagaacagct acttattgat
     7861 ttaacttctg caaaaggagt acctggaaat gaagaagagg taagagaagt attcagagaa
     7921 tatgccaaac cattcgctga tgatattttt tatgacggtt taggcagtgt gattgccaaa
     7981 catggtgctg gcggaggccc caaggtcttt atttctggac atatggacga agttggtttt
     8041 atggtaacaa aaataaccga aaaaggattc ttggaattcc aaactcttgg aggttggtgg
     8101 gggcaagtca tgcttgctca gcaagttgaa atcaaaacac gtgaagggaa aatcgtccac
     8161 ggtgtgattg gttccaaacc accacatgtt ttaacacctc aagtaagaaa taaaccttat
     8221 gaaataaagg atatgttcat tgatattggc gcatcaagcc aagaagaagc caaagagtgg
     8281 ggcatacgtc ctggagatat ggtcactcca tatattgaat acaaacgaat gaatggcagt
     8341 aaatatttac tggcaaaagc atgggataat cgtatcggga cagctgtttc tctgcgagta
     8401 ttagagaatc tatctaaaga agcacaccca aatgttttat tcgcaggaag cgatgtgcaa
     8461 gaagaagtag gcttgcgagg tgctcgaaca agtacacacc ttgtaaatcc cgatattgct
     8521 tttgctttag atactggtac agcaggagac actccaggaa tgacaccaaa agaagcagat
     8581 tcgattttag gaaaaggccc acagattttg atttttgatg catctatgat cccgcataaa
     8641 aaattattga attttgttat tgatgtagca gaggaaatgg atattccatt ccaatataca
     8701 gtgatttctg gcggaggaac tgatgcagga caaatgcatt tgacaagaga tggtgttccg
     8761 tctgtagcca ttaccgttcc tgttcgttat ctgcattccc atacatcagt gattcatgaa
     8821 gatgattatt tgaatacagt gaaactagtc acagaattag tgaaacggct tgatagtgaa
     8881 acagttgaaa aaattcggag ttatgaataa tgaataatag aatacctatt tctgaatatc
     8941 ggaaaaaagc acgagtttct ttggaaaatc aatggggaat caatacttgg cttgttttct
     9001 tatctataat atttacgagc gtcatccaga acttttttga cggtttcagt ccttttccgg
     9061 aagaaagcat gcagcagcac ttattgagtt ttctagtcga taatttactt ttattcgcat
     9121 tcaactatgc attgttttac attgcgctag tagttgtacg gggtggcaga ccaaaaagta
     9181 accttttatt ttcagttttc caaagaaagt attttggttc gatacttggc atcaatttga
     9241 tcaattcagt catcaactgg ctactcaaca gtttaatctt tctgcccatg tttttaatca
     9301 gcggagtgag tacttatatg cagctgcttt tcagttcaaa tgcggtacct acgggtgtca
     9361 tgccagaaga ttttcttgat atcagctttg ttttatcgat gatagggatg atttttgttt
     9421 cagcactcat catgagtatt gttggcggtt tattccaatt tgctgcatgg acaaagttcg
     9481 actatcctga tttgtcggtt attcaatgtt tgaaatatgg ctggttttta ttgaaagata
     9541 gaattggtac gtatattttg ttgcagctgt cttttatcgg ctggtatttg ctaggagcaa
     9601 tcgctttgtt catcggtctt ttgtgggtca ttgtttatgt gaatgtgtca gttgcagaat
     9661 tttatgaaca agcacggatt gaaaaaggag gtccagacgc ctattttact tagcatgtaa
     9721 gcagtttatt catcaaaaaa ataacttttg tggtaggata aatctttgag ggggaatgta
     9781 cgatgaaatt aactgtttta ggatgcttag gagcgtatcc ttataaaaat caagggacaa
     9841 caggttattt gcttcaatct gacgggttca atttgttgat cgatgcaggt agtgctacgc
     9901 tgatcaaatt acaagaacac cttgatccac ttgatctaga tgctgtgatc cttagccatt
     9961 atcatcatga tcatattgct gaccttggtg tattacagta ttactggcaa cttcatccta
    10021 agagaaatcc ggagacattg ctttcaattt atggacacac aaaagattcc attcactttg
    10081 aagaattgac gttggaaaat gtctcaaaag gacatcctta ttttgaagct gaagaattaa
    10141 aaattggtcc ttttctgatt acttttatgg aaacaatcca tccagtagta tgttacgcca
    10201 tgcgtatcgt agaagaaaca acggggaaag tattcgtatt tacaggtgat tcaggttatt
    10261 tggaaagttt cgctgatttt gcgaaagatg ctgatttgtt tctagcagat acctatcttt
    10321 ttgaaggaaa tgaacatcat catgcccatt tcacttcaaa agaagctgga gaacttgcga
    10381 aatcagctaa cgtaaagaaa cttgtactta cacacctacc tcagtttggt gatttagaac
    10441 aattgaaaaa tgaagcgcaa aaggcagcag gcaaagggat tgatatagaa cttgctgcag
    10501 ttgacaaagt gtttaacatt taggaggaag aacatgattt ttgtaccaaa tgaaaaccac
    10561 gacccaagaa tcaatttagc aattgaacaa tatttgctgc aagaaatgcc tttagatgaa
    10621 cctattttgc tcttttatat caatgaacct tcaatcatca tcggcagaaa ccaaaatacg
    10681 cttgaagaaa tcaatcgaga ttatgtggaa gaaaatggca ttcatgttgt ccgtcgctta
    10741 agtggaggcg gagctgtcta ccatgatttt ggaaacctga actttagttt tatcatgcct
    10801 gatgatgggg attcattccg tgactttgcg aaagtaacac agccaatcat ccaagcactt
    10861 catgaattgg gtgttgctgg ggcggaattg aaaggccgaa atgatctagt cattgatggc
    10921 atgaaatttt ctggcaacgc gatgtatgct accaacggtc ggatgtttgc tcatggaaca
    10981 attatgttcg actctgatat caatgaagta gtcaatgcat taaaagtaaa aaaagacaaa
    11041 atcgaatcaa aaggaatcaa atcgatccgt tctcgtgtaa cgaatatcaa accatttctt
    11101 ccagaagaaa agcaaaaaat gacaacagaa gaatttcgtc aagaaatctt gttgaaaatt
    11161 ttcggcgtag agtcagtaga agaagtaaaa acgtacgaat tgactgatga agattggaaa
    11221 aaaatcaatc gaatctctga ggaatattat cgaaattggg attggaatta cgggaagtct
    11281 ccagatttca atttctctcg ccaaaaacgt ttttctatcg gttcgattga agtccatttg
    11341 aatgtctcag aaggaatgat caaagaagca aaaatctttg gcgatttctt cggactagga
    11401 gatatctctg acgtggaaaa aaaattggtt ggacagaaat atgataaagc atctctatca
    11461 aaaatagtca gccaaattga tgtgaagaaa tattttggtg cgatagaacc agaagaattg
    11521 cttaccttac tttattgaaa ataaaaaata gcggaatcca catatatgga ttccgctatt
    11581 ttttatacag gattcgggta atttcgagag agttgaacag tgatccgatc aatgaaagta
    11641 tctttttcct gcgttagtgt tcctgtgtaa cgatccgcta cgtctttcat catcattaag
    11701 tcaaaacgtt cctgatcatc taattcttct gtgatgcctc gactaaatag gtttgtatcg
    11761 taatgtgcac tatgttcgca acaaatgacg aaatgttcct cttgttcgtg tatcgacaaa
    11821 taaatttttc gatacagtgg attttcgatg gcatgagttt ctctaaagga atgctctaaa
    11881 gcgtggatca aaagttgagc cagatcgtca tcgttgatag gaagttcttc gggaagatct
    11941 gcttggaagg ttatctgaat attccgtttg gcagctaact tctggtaacg cgctaagata
    12001 agatttgtca cctggtggtt gcaaaaagaa cacagcattt cgaaattttt tgcttcttct
    12061 aatatctttt ccacatagtt tttagctttt gtttggtccg tatgcattaa atcttgcaga
    12121 ttctccatct cttggacaaa ttccaggcga agtgtattta cttcttggat atgctgtcga
    12181 agctgctcat aatgttgctc caacaaatca tttttgattt ttaagaaatc tacttgtctc
    12241 cgatctttgt cggtatttaa gatgtgatcg attagattgt agctgataat aacgattaga
    12301 atgataatat ataaaatcgt agaccaattg atcgttgttt gatagatacc taatgccgtt
    12361 tggatgtaaa tcaagcaatg gataatggct accaagacga tccaaggtgt acaagctaca
    12421 taaaaacggt tattcttata tgcttcgcca atgcatgcaa gtgaagtgac taaagtgctt
    12481 ataacactag caatattagc aatcggcatt aattcaggaa gttctgcagg agtaaaagtt
    12541 gcataaagca gaacgccgat aaaaatcaaa agttggaacg tacaaaaaat tccgtaccat
    12601 tttttatagt aaaacattcg gctccaatag tagcagatga tgaaagctga cgttaagata
    12661 ataaataaat gagataaaaa agaatgaacg aatggttcaa aaagaatacc acttaaaata
    12721 tcttctgata cagcttcaaa tcctaatgcc aaagcaaagc aacttaagag aataagtgag
    12781 tattcaagac gtttttgttt atacatcaag tagatcgtat agaaaaaggt ccctatagcg
    12841 ataaaaacag cgattatcat cgtcaatact tgcggcactg attgtgaata aataaaacta
    12901 atcaatggtc cagttgttcc tagataaact ttcggtggca gtccagcata gttctgataa
    12961 ggagatgaga ctgtaagggt caattttttt ccgatataat ttgctggtaa atctatcaca
    13021 gccaatgaga gtccgggatt gctttcttcc tgataatctt tccgttggta taaaatcttt
    13081 tcgcctagat atacagtaat ccattgatgg tttcctttga ttagtaaatt tgcttcgtcc
    13141 aacggttttt caagtgtcgt ttccataatg aatgtttcgt ctttatccac ggtaggaatc
    13201 gtacgagtgt acttgctgtc aaacttaaaa tcaggatcat cttttgtata gaaaacccac
    13261 gaatctttta tttctttcat ttgattgctg tccgcaggag aaaaaataaa ataaaagaaa
    13321 taagcaattc ctgttaaaaa taacaatgat aatataatga ttatgct
//
LOCUS       DAYYCY010000070        13318 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000070 DAYYCY010000000
VERSION     DAYYCY010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13318
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.070"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            207..1946
                     /gene="yfcC"
                     /locus_tag="ACHAZB_001579"
     CDS             207..1946
                     /gene="yfcC"
                     /locus_tag="ACHAZB_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFM8614253.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            2103..3287
                     /gene="iadA"
                     /locus_tag="ACHAZB_001580"
     CDS             2103..3287
                     /gene="iadA"
                     /locus_tag="ACHAZB_001580"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFM8614254.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3398..3601
                     /locus_tag="ACHAZB_001581"
     CDS             3398..3601
                     /locus_tag="ACHAZB_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFM8614255.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3702..4364)
                     /locus_tag="ACHAZB_001582"
     CDS             complement(3702..4364)
                     /locus_tag="ACHAZB_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8614256.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4377..5879)
                     /locus_tag="ACHAZB_001583"
     CDS             complement(4377..5879)
                     /locus_tag="ACHAZB_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8614257.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6427..8106
                     /locus_tag="ACHAZB_001584"
     CDS             6427..8106
                     /locus_tag="ACHAZB_001584"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFM8614258.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8233..8712
                     /locus_tag="ACHAZB_001585"
     CDS             8233..8712
                     /locus_tag="ACHAZB_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HFM8614259.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8931..9611
                     /locus_tag="ACHAZB_001586"
     CDS             8931..9611
                     /locus_tag="ACHAZB_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFM8614260.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9619..10950
                     /locus_tag="ACHAZB_001587"
     CDS             9619..10950
                     /locus_tag="ACHAZB_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HFM8614261.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10965..11927
                     /locus_tag="ACHAZB_001588"
     CDS             10965..11927
                     /locus_tag="ACHAZB_001588"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HFM8614262.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            12038..12376
                     /locus_tag="ACHAZB_001589"
     CDS             12038..12376
                     /locus_tag="ACHAZB_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFM8614263.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12455..13162
                     /locus_tag="ACHAZB_001590"
     CDS             12455..13162
                     /locus_tag="ACHAZB_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HFM8614264.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
BASE COUNT         4258 a         2340 c         2915 g         3805 t
ORIGIN      
        1 attttaagga aaaacagaga aataaaatac ttaatataaa tggaaagaga aaaaaataat
       61 catacatagg agaaaaaaaa caaaatagaa aaaagtgatt ttctaataaa ttattcatgt
      121 ggaaatgtca tttttactgt ataattcatt tgattatcca ttttttattc atcaaaatta
      181 ctgaaaaaag aaaggaagaa ataaatttga agaaaacaaa ttcttcagga aaaccgaaac
      241 gcgacttcac taaattatcg acaccccata cgtatgtcat tatttttggt gtagtcatct
      301 ttgcatggat actgactttt gtcgttccag caggaaagtt cagcacgcaa gatattgaat
      361 acaaagatgc aaatggcgaa acaagtactc gtactgtttt acgtcaagat tccttccgtt
      421 atgcttacga gctggataag tcgtatgtgt ttgatcaatt agaagaactg caagatcatc
      481 cagcagaacg tgaaaaactg gatgttccgg aaaaaggatt ggaaaaagtc attgctgacg
      541 gagaaaaaaa tcttacgcag gaaaaactag atgaaatctc cttgacagat gatgtgcttt
      601 acgatcagta cggcgaaaat atctatgata cttctaaaaa attgcataaa acagccaaaa
      661 tatggggaac agatgatttt ggcggctttg gtttcttaaa ttttgttttt gaaggactag
      721 tatctggaga taagtatgga tctgctgtag ggatcgctgc attgatcctt gttgttggag
      781 gtgcttttgg tattattatg cgtaccggag cgatcgacgc aggtatctat gcgtttatca
      841 gtaaaactaa gggactggag cgtttagctt taccgctatt attctttgct ttttcattcg
      901 gtggcgctac attcgggatg gcagaagagg taattccatt ctcaatggtg atggttccat
      961 ttgtcattgc gttaggatat gactcgatcg tagcagtaac cgtgacttat gtcgcatctc
     1021 aagtcggaaa tgcgacctct tggatgagcc cgtttagcgt agcggtggct caagggattg
     1081 ctggaatccc cgtgttgtct ggtgcgacat tccgattgat tatgtgggta gttgttactg
     1141 cattagccgc tggttatatg atgatatatg cagaaaaaat ccgtaaaaaa ccagaaagtt
     1201 cgttgactta caaatcagat gattatttcc gttctcatat caaaaagaca tctgatgaaa
     1261 ataaaaaatt catgcttggg cataaattga tcttattaga gatgttagtt gtccttgtct
     1321 ggatcgtttg gggcgtgact caaaaaggct attacattcc agaaatcgct tctcaattct
     1381 ttgtgatggg attagttgct ggaatcattg ctgttctttt caaattagat ggaatgaaag
     1441 tcaatgacat cgcttcttct ttccaatctg gtgcagctga tttagctggt acagcaatcg
     1501 tagtaggtat ggccaaaggg attcttcttg tcttgggagg atctgatgca tctgttcctt
     1561 cagctttgaa tacgattttg catgggatcg ggacggcgtt gaccggtgtt ccagctgtca
     1621 ttggtgcttg ggcgatgtac atcttccaaa gtttgttcaa cttggttgtt acatcaaatt
     1681 caggacaagc agctttgact atgccaatca tggctccact ggcagattta gtaggtgtat
     1741 ccagacaagt agcagttctt gcttatcagc ttggttcagg ttttatggac gcttttacac
     1801 ccgtatcagc cagcttgatt ggtgtgctag gtgttgcacg gatcgaatgg gcaaaatggg
     1861 caagattcca aatcaagatg caaggattcc tgtttgtttt aggaactatt ttcatcatga
     1921 ttgccattgc aataggtctg cagtagtact ggagacctgt aaataaaaaa gtaatccaaa
     1981 cagtagataa ggactccaag aaccgtcatt ttacggtaaa atcaaaggag taaagatcct
     2041 atcgtttgga ttatttttta agatcctatc atcagttata cttaaagaaa gaggaaaaat
     2101 gtatgaaaat cattcgacaa attgaagtgt ttgcaccaga ttacttagga aaaatggatg
     2161 tactgattgc cggtgggaag attttggctg tagaagaaca gctagaaggc ggctacgaag
     2221 gagtggaagt agaagaactt tcaggtgagg gaaaagtact aaccccgggt ttcattgact
     2281 gtcatttcca tattttaggt ggaggaggag aaggcggtta tcaaaatcga acgccagaag
     2341 tcacattgag ccaattgact acagctggtg taacgacagt tgtcggttgt ttgggaacag
     2401 acggagaagg tagagatatg actgcgttga tcagcaaagc gaaaggtctg gaagcagaag
     2461 gaatctccac ttatgtatac gaaggttcgt accgattgcc ggtaaaaaca gtaacagatt
     2521 ctatcatcaa agactttttg acaattgata aaattatcgg aatcggagaa atcgcagtat
     2581 ccgatcaccg ttcttctcag ccaagttttg aagagttcgc tcgtgcagta gcagatgctc
     2641 gtgtaggagg catgttatcc ggaaaagctg ggatcatcaa tgtccactta ggcggaggaa
     2701 aaagaaagct agagctattg acacgaatcg tagaagaaac agaaattccg atcacacaat
     2761 ttttgcctac tcatgcaaat cgcacaccag aattatttga agcttgcgtg gcatttgcca
     2821 aacgtggtgg cacgattgac ttcactgcta gcgaagatcc tgatttttgg gagaagacag
     2881 atggtgaagt tcgcttcagc aaagcactga aacgattgat cgaggaagat gtttctttag
     2941 ataatttcac gatgacctca gatggacaag gaagcttgcc ttattttgat gaaaacaatc
     3001 atttcctagg gttaggagta ggaagtgcca aggcattgct agtagggatc aaagaagctg
     3061 tgcaaaaaga aagtatccct ttggaaatcg ccttacgagc tataaccagt aatccagcac
     3121 gtatattgaa attggataaa aaaggaaaaa tcgaaattgg tgctgatgca gatctgtgta
     3181 tcttggataa agaaacgctt gatattgata cagttatcgc taaaggagaa ataatggtcc
     3241 aagaaaaaga agtcaaagta tgggggactt tcgaaaaaag tttttaatca acagctctaa
     3301 ctatcttttt tagaaaacga gcgttctgtt tgtctgtatc tgttgttgat gttaccctga
     3361 aggtagatca ataaacaaac taagaggagg aagcacaatg gcagatttaa aaggacgttc
     3421 taaagatatg aaagataaag tagtcggtga aggaaaagaa gcatacggca aagcaacaga
     3481 cgacaagagc aaagaaacag aagggaaagc acaatcacta ggtgctgaca tcaaagaaaa
     3541 agctcgaaat ttaggcgacg aagtcaaaga caagttttct cgaaaagacg aaaaaaaata
     3601 aaaaccactt attactaatt gaatcaagca aataaaaaat ccgaaccata tctgcgagac
     3661 agatatggtt cggatttttt atttgatcat tatcctgttt tttaaacttc gattttttga
     3721 aattttttat tttttagttc aaagacttca tcacagcttt ttgctacttc aatctcatga
     3781 gtcacgatga taatacagcg attttgttca tgggcgattt cttggaaaag tttgacgata
     3841 tcttgtgaag ttgtttcatc tagatttcca gtaggctcat ccgctacgat tagttgatgt
     3901 tcacagcaga tagcacgaac gatagcgact cgctgctgct gacctcccga aagtttggtc
     3961 acctgttttt ttgctaatat ttcatcaatt cctacacgag ccagtcgatc taaagcaact
     4021 tgtcgataat tctcttgctt ggtcttagtg atttccattg ctgctatgac gttgtctaga
     4081 gccgataaat aaggaagcaa gttgtaagct tggaaaataa tcgagacatc atttttacga
     4141 taacttctta acccgatttt gttcagtgaa cgatcttgat agtatacctt tccttctttt
     4201 ggtgcatcta gtccagaaat aagcgaaaga aaagtcgttt ttccagaacc acttgctcct
     4261 aaaatagcgt acatttttcc agcttcgaaa ttcagttcta catctttata aagcgcatct
     4321 tgcggatctt gataccaaaa tcccaattgt tctgtccgta acatcaaatt cctcctttag
     4381 tttaatagga tttttcttgg gtttaatctt aatatcccta tagaagctaa aaagacagaa
     4441 aacagactga ttccaaatgc tactccggct aatagtccca attgtgcagg tttgacattg
     4501 atttccaatt cgctgacttg atcagaaaca gcaaatggat tattcgtggt tccttgttga
     4561 ccatcgcgtt gaccgccttg ttcttgtctg ttacctggca tttgtccacc gccaggagct
     4621 tgctggtttt gtgtcgtggt ttctgtctgt tggtcaagta gctgctgtcc cacggcattc
     4681 gcgacgacgt ttccgctaag cgaagcaact cctaatgcaa gaatcataca aatagccact
     4741 tcggtaaaca attggagaat gattttactt ctggattctc ctaaagatag caagacgcct
     4801 aactcatgcc gacgctcccg aatggtaatc ataatgatca gagtaagtat gataataccg
     4861 gctacagcca ctagtaaaac aacattttta gaaaaacttg ccacgttgtt taaaggttcc
     4921 aacattgact gatacatgga atcatttgat tgtaagctaa atgtatctgt gtcgatcagc
     4981 ttctcagctt ctttgagaaa ctcatccatt ttatctggat cagatagagt gtaaatagca
     5041 gaatctatcg tgttatcgtc agatgtccca tttagttcat ttgcaaaagt gtaagaagtg
     5101 taaagtgtat tcgacggatt catgaagttg aatttcatac ccatactgcc gcttgtttcg
     5161 ctggattcat aaattccttt gatcgtcatt tcataagtgg tatcttcatc ttcagtactc
     5221 gtgattgtga aagtatctcc gactgccagg tcatttgttt ctgctagagt agaatcaatg
     5281 actacattgt ttgtgccttt gtcgctgtca tctattcctt caccatccgt gatttttgct
     5341 gttccatcag aaaaattact attttgtgca gttgctgaca caccactgat ttgaaagtct
     5401 gcttgagtca tttgcggatt cccgcccatt cctccagcag gttcaccgcc tgcatcagta
     5461 gtttctgaac tgtcttcact tgaatcagaa ctagtgatgg cagttattcc atcttttgcc
     5521 atagctgagg cggaactttc aaaggaataa ctctttacat tatctaattc agctattttt
     5581 tccgcgtccg ttaaggaaac tggtgtaaga gaaaaactgc ctggatctgg acgttcttca
     5641 gtggtagaac cgcttgaaga agtttgttcc tgttttttaa atgaagcttc tcgattggca
     5701 cttaaagtca ctgttgctcc aacttctttt tgtgcttgat tcgctgcttc ttctgcagcg
     5761 cttcgaatcg tgaggccagc tagcacaaaa ataagaatgg ctgtgaacac ggcaaacaat
     5821 aacaatgaac gtccccattt tacttttgta cttttccatg cacgtttcat aaaattcata
     5881 ttttgcatcc ctcctagtag atgcatttga taatagacaa cctatcttaa agctagctta
     5941 aatcttatgc tgtttgcaca ccatcaacat gaggttctgt atatataaaa agtcactata
     6001 gttattgcaa aaaatgtggt aaaatgaaaa aagtatgttt caaaccgacg aagaagaaat
     6061 gaaagaggaa tagcaaaaga aatcctagat gctacagagg gatttttacg tgcatagttg
     6121 ttctaacaat tgctgatact agcgatttag tttatttgtt gtataataat ggggaacagc
     6181 tgaatactta tataagtatt ctggctatag cgtttcgcaa aagatccatt ttgttaatga
     6241 agtatatcca aaataaaatg cccatccaac tgtcagaacg cggatgtgct ggtaattttc
     6301 ctgctgattc tccataagtt gagaaatagt gattttgcat atgcttcatt ggaatggctc
     6361 atatatttag ataaagaaga aaatcgacat ctttaaactt ggatgtcata agaagaggtg
     6421 aagtttgtga gtaccattaa aattatccca ttaggcggcg ttcgtgaaaa tggaaaaaac
     6481 atgtatgtcg cagaagtggg cgaggatatt tttattttag attgtgggct aaaatatcct
     6541 gaaaatgagt tgttggggat cgacgtcgta atccctgatt ttacgtattt agaagaaaac
     6601 attgatcgtg tagctggtgt ctttttaaca catggacatg cagatgcgat cggggcactt
     6661 ccttacttgt tgtcaaaaat ttctgtacca gtattcggga cagagttgac gatcgagtta
     6721 gcaaaattga atgtcagtcg taatgaagca gccaaaggat ttaaggattt ccatgtggta
     6781 gatgagcata cagaaataga ctttggtgaa acagtagtga gctttttccg taccactcat
     6841 acgataccag attcagtggg gatcaatctg aaaacaaaag aaggtagtat cgtttatacg
     6901 ggagatttta aatttgacca aacggctatc ccaatgtatc aaacagatta tgcacgattg
     6961 gcggaaatcg gcaaagaagg agtcttagcc ttactaagtg aatcggcaaa tgcagaaaat
     7021 cctcagcctg tagcttctga agcccaaata gcagatgaag tatttgatac gatcaaatat
     7081 tgggaagggc gtatcatcgt cgcttgtgta gcaagtaatt tgcaacgagt gcaacaagta
     7141 ttggatgcag cagaaaaagc acaacgaaaa gttgtcttga ctggtcaaga tttcgaacga
     7201 attattcgca cagcgatgcg tttagaaaaa cttcaattac caagtgaaga tctgcttgtt
     7261 acgttgaaag acatgaaaaa ctatgatcca gaagaactga tcattttaga aactggccga
     7321 atgggtgaac ccatcaagtc tctgcaaaaa atggcaaacg gtacacaccg taatgtcaag
     7381 attgaagaag gagatttggt ttatatcact actacgccaa gcatcgcgat ggaaaccatc
     7441 gtggcgaaaa cggaagatat catttatcga gctggtggaa cagtgaagtt gatctctgag
     7501 aatatgcgcg tttctggcca tgccaatcct aatgacttgc aattaatgat caatttgatg
     7561 aaaccaacat attttgtgcc agttcaagga gagtatcgtg aattagctgc tcatgcagat
     7621 ttggctcatg cagtagggat gccatacaaa aatatctata ttactggacg cggagacgtg
     7681 ctggaatata agaacaagcg tatgagtgtt gcaggaacta caagtgcaga aaatgtaatg
     7741 attgatggat taggcatagg tgatatcgga aacattgttt tacgtgatcg aaaaatcttg
     7801 tcagaagacg ggattttcgt agcggttgtc actatcagtc ggcgagaaaa gaaaatcatt
     7861 tcaaaacctc aaattacttc acggggattc gtttacgtaa aagctagccg tgatctgatg
     7921 aaagaaagca gcaatatgat tgaaacaatc gttgagaaac atttagaaag caatgatttt
     7981 gagtggagca aattaaagca agacatccga gatcaattag gccgttactt atttgaacaa
     8041 acaaaacgcc gcccagtgat cttgccagtc attatggaag ctactcaacg tcgaaataaa
     8101 aaataaacaa aaagagtaag atgaacctct ctatagagtg ctcgtcttgc tctttttttg
     8161 cgaaaaacaa ataatttttg aatacagcat ccctcgcttt ataataaacg atagaataaa
     8221 gggagtgaat caatgatgag tcaaacaaaa gtcgaacgtt atttagaaga acaaaagatc
     8281 agctatcaac cacttgattt tagtcatgtg gtagaagaat ctttcacaga agaactacaa
     8341 aaaagaggga ttgatccagc tttgatatgt aaaactctcg tagtgaaagg aaacaaaact
     8401 ggggtgatca tcgcattgat tcctttgaac gatcacttag attacaaaaa aacgagacaa
     8461 gtgacgaaag atcgtaaagt tggttttcct ggaatggatt ttgtattaat gcatacagga
     8521 tatccccacg gagcaaacac ccctattgga ataaaactcg cacatcctga ttattgtttt
     8581 ttagcagaca gttctttaaa agaaaaagaa gaagtaattg tgtcaagcgg tgaaatcggg
     8641 agaagccttc ggataaaagt agccgattta gaaaagatcg tccatcctat ttacgctgat
     8701 ttaattagtt aagggacgag aagagaagct aagcagagca gagacctcaa acttgagatt
     8761 atatctgctt ggtacttggt gcatcctgta ttatctgtta caattgcttt gtacatactg
     8821 tactatcaat ccaatgaaaa tatatttagc agctatattt tttcattgat gtgcaagtga
     8881 tgaaatcgtt tttaataaaa aagatacaga gaaaacgcga ggtgtaaaat atgaaaatta
     8941 cgtatcatgg acattcctgt ttaacaattg aaatggaaaa tggccaacgc gtgatgattg
     9001 acccgtttat tacaggaaat ccattgacag acttaactgc agaagaagta aagacagatt
     9061 ggttgctgat tactcatggt catagtgacc acataggaga tatgattcct attgcaaaag
     9121 aaaatcaagc cacaattatc agtattgttg agattgctga ttatgcgaac agcagaggag
     9181 tagattcatt cggtatgaat atcggtggaa aacatgcgtt tccattcggc gtggtaaaat
     9241 tcgtacatgc acagcatagt tcaagctatg aagtagacgg tatcgttcag tatatgggcg
     9301 agcctagcgg aattattatt caagcagaag gaaaaacgat ttatcatgca ggggatactg
     9361 cttatttcag tgatctagga ttgcttgcag aagaattcga tattgatgtc gctttcttgc
     9421 cgattggcga taactatacg atggggccag aagatgcagc aaaggctgcg caaacaattc
     9481 gtgccaagaa agtagttcca atccattaca atactttccc tgtcattgaa caagatcccg
     9541 aatcttttgt agagttactg gaagatagtg aaggaaaaat aatgagcgtc tcggaaacgc
     9601 tggaagtgta ggcagaaaat ggcgacaaaa catgatttga ttttagaata tattgaaagt
     9661 ttacctgttg gcaatcgaat ttctgtaaga agcatcgcaa aagaattgaa tgtcagtgaa
     9721 ggaacagcgt accgtgcaat caaagatgca gaaaatgtcg gactagtttc aacaattcaa
     9781 cgagtaggga ccatccgtat cgaacgcaaa ttaaaaaaac atattgaacg tttgacattt
     9841 ggcgaggtcg tcaggatcat tgaaggggac gttttaggcg gggcggcagg attagacaag
     9901 gtcttgaata aattcgtcat cggcgcgatg actaaacatg ccatggaacg ctatattacg
     9961 ccaggatcac taatgatcgt cggcaaccgg gcggaagtcc aaaaattagc attggaagat
    10021 ggcgccgcag tattgatcac aggaggattc aatacgactg ctgagattgc aaagctagct
    10081 gatgatcttg aaatgccgat cttacgcacc tcttatgata catttacagt cgcaaccatg
    10141 atcaaccgag cgttaagcga ccaattgatc aaaaaagata tcatgcttgt cagtgatatc
    10201 tatatgccag ctgaaaagac tcattatttg cagcagacag atacgatttc tgattatcag
    10261 cgtttgtcag aagaaacgca gcattcgcgt tttccagtcg tcaaccgcca tcatcgtttg
    10321 atggggattg ttacggcaaa agatgtttta gggaaatcgc caaaccagat catcgatcgg
    10381 gtgatgacga aagaaccaat cagtgtaaag aaaacaatga gtattgcctc tgttagccat
    10441 caaatgatct gggatgggct agaagtcatg ccggtcgttt ccgatgactt gacattggaa
    10501 ggcttgatta cccggcaaga tgtaatgaaa gcgatgcagc ttgtccagcg acaaccgcag
    10561 attgcagata cgatttcgga tcagatatct ggaaatatcc atacgattga tactgaccgt
    10621 gaaggaaatc gtttggaaaa ccctaagttc aaattcgtag tagcaccaca aatggtcaat
    10681 ggcgttggaa ccatttcttt tggcgtactt agcgaaatca tttctgatgt cgcacaaaaa
    10741 acaatggtga tgaaccaaaa acgaaatatc ttgattgaac aagtgaacct acattatttg
    10801 cggctgatcc aattggaaag cgaattagac attcgtccac gtgtattgga aatcggcaga
    10861 cgttccgcta aactggatat cgaagtgttt attgaaaaca ccatcgtttc caaagcaatc
    10921 gttgtttgtc aagtaatgga acgttcttag aaagcaggag tgaaatgaca gtacagcaag
    10981 aaatagtaga tacaataaaa aaatacgata aaatcattat ccacaggcat ttgcgccctg
    11041 atcctgatgc attaggatca caagtaggat tagccgaact attacgggaa agtttcccag
    11101 aaaaagaaat acgtcaagta ggtgaaacga ttgaagggct tcgcttttta gcagaaatgc
    11161 aagaagttga agatgtcttc taccaaggag cgctagtcat cgtgactgat acagctaatt
    11221 cacccagaat cagtgatcaa agatatcagt tggcagaaaa actgatcaaa atcgaccatc
    11281 atcccaatga tgacccttat ggagatcttg tgtgggtaaa tacaaaagca agtagctgta
    11341 gtgagatgat tgcagaattt gctttgatgt ttcctgaatc tttaaagatg aacgttaagg
    11401 ctgcacggtt actttatgca ggaatcgtcg gagatacagg gcgctttttg tatccctcca
    11461 cgacgtcccg tacattagag attgcttcga tattgagaaa ttatccattt gatgcaagtg
    11521 ctttgaacag agagattgaa caaatgccga tgaaagtcgc taaactttcc ggttatcttt
    11581 atcaaaatat ccaggtggat gaaaatggtg caggaagaat aatccttccg caagaaattc
    11641 ttgataaata tggaatcgat gattcagaga cagcttcgat cgtttcactt cctggagtga
    11701 ttgacgaagt gctggcttgg gggatttttg ttcagcagcc ttcagggtat taccgcgtac
    11761 gtttacgttc taaggggccg atcatcaatg aattggctaa acgccatcat ggaggaggcc
    11821 atccattagc aagtggtgcg aatgcaaaag atttagcaga agtagaggaa atctataaag
    11881 agatccaaga tatatgccaa tcgtataaga gcagaaaaac agaatgatac ttcgttcgaa
    11941 aacgtgcctt ttgtagtaaa aatcacaaat gtatcttttc aagatcttct ttttccgtta
    12001 caatagtgct cactaaagat tttaaaggag aaagaagatg acattaccaa attgcccaga
    12061 atgtggctca gagtacacat atgaagatcg aggcttgttg atttgcccag aatgcgggaa
    12121 cgaatggaac aaagaagaac aagaaactgt catagaagac ggattagtgg tattagatgc
    12181 aaatggaaac cgtctgttag agggcgatag cgtgacagtg attaaagact tgaaagtaaa
    12241 aggtgcgtca ggtgccatta aacaaggaac aaaagtcaag aacatccgtt tagtagaagg
    12301 cgatcataat atcgattgca aggtagaagg atttggaccg atgaaactga aatcggaatt
    12361 tgtgaaaaag aattaaagaa tattcaaggt cttttcacat tcttttccta ctactaatga
    12421 taaaataaag taaacgaaaa ggagtgaaac gaaaatgtat ggttatggaa tgtatggttt
    12481 tttagatccg acatttcttt tggtcattct tggattagtt atttccggaa ttgcgtcagc
    12541 gtacgtcaat agtactttcc gaaaatacga tcaagtgaga agcaaaaaca atgtgactgg
    12601 tacacaagca gcacaatata tattacaaag tcaacagata aataatgtcg gtgttcaaca
    12661 aattgccgga gatctcacag ataactacaa ttcaggtaat aagatgttga gtttgtctca
    12721 agcaactgca cagtctactt cagttgctgc gataggtgta gcagctcatg aatgcggaca
    12781 tgcggtacaa gatgcttcgg gttatgtccc attgaagatt cgtgcagcac ttgttccagc
    12841 agctaacttt ggttcgatga tttcgttccc cttgattatg gtaggtgtat tgcttagctg
    12901 gaactctacg ttaatcaata tcgggatttt cgctttttct ttagcattac ttttccaatt
    12961 ggtgacatta ccagtcgaat ttaacgcgtc tcggcgagca atccaaattt tgagtgaagg
    13021 cggactactg acggaagaag aagtaccgat ggcaagacac gtattatttg cagcagcact
    13081 gacttatgtt gcagcagcgc tttcgacatt cctgcagctg ttgcgcttga tcttattgtt
    13141 cggcggcaac cgaagagatt aaaataaaga agttgtgaaa tcagcgcttt gatctgagct
    13201 aataccgaga gttagctgat tgaataccat ttatttggaa taagggctct ttgtcaataa
    13261 ggactgatgg gttgtacaaa atcaaatctg ggtcagaatg aacctcattc tggctcag
//
LOCUS       DAYYCY010000071        13200 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000071 DAYYCY010000000
VERSION     DAYYCY010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13200
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.071"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(80..1891)
                     /locus_tag="ACHAZB_001591"
     CDS             complement(80..1891)
                     /locus_tag="ACHAZB_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HFM8614265.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(1888..2253)
                     /locus_tag="ACHAZB_001592"
     CDS             complement(1888..2253)
                     /locus_tag="ACHAZB_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFM8614266.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(2416..2811)
                     /locus_tag="ACHAZB_001593"
     CDS             complement(2416..2811)
                     /locus_tag="ACHAZB_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFM8614267.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(2829..3791)
                     /locus_tag="ACHAZB_001594"
     CDS             complement(2829..3791)
                     /locus_tag="ACHAZB_001594"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HFM8614268.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(3791..4003)
                     /locus_tag="ACHAZB_001595"
     CDS             complement(3791..4003)
                     /locus_tag="ACHAZB_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HFM8614269.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(4024..4722)
                     /locus_tag="ACHAZB_001596"
     CDS             complement(4024..4722)
                     /locus_tag="ACHAZB_001596"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFM8614270.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(4742..5284)
                     /locus_tag="ACHAZB_001597"
     CDS             complement(4742..5284)
                     /locus_tag="ACHAZB_001597"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFM8614271.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(5416..6420)
                     /locus_tag="ACHAZB_001598"
     CDS             complement(5416..6420)
                     /locus_tag="ACHAZB_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFM8614272.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(6417..7406)
                     /locus_tag="ACHAZB_001599"
     CDS             complement(6417..7406)
                     /locus_tag="ACHAZB_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFM8614273.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVEKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(7403..8209)
                     /locus_tag="ACHAZB_001600"
     CDS             complement(7403..8209)
                     /locus_tag="ACHAZB_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8614274.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            8375..9331
                     /locus_tag="ACHAZB_001601"
     CDS             8375..9331
                     /locus_tag="ACHAZB_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8614275.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(9408..9926)
                     /locus_tag="ACHAZB_001602"
     CDS             complement(9408..9926)
                     /locus_tag="ACHAZB_001602"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFM8614276.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(10014..10163)
                     /gene="rpmG"
                     /locus_tag="ACHAZB_001603"
     CDS             complement(10014..10163)
                     /gene="rpmG"
                     /locus_tag="ACHAZB_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFM8614277.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            10391..10837
                     /locus_tag="ACHAZB_001604"
     CDS             10391..10837
                     /locus_tag="ACHAZB_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFM8614278.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(11032..12927)
                     /gene="asnB"
                     /locus_tag="ACHAZB_001605"
     CDS             complement(11032..12927)
                     /gene="asnB"
                     /locus_tag="ACHAZB_001605"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HFM8614279.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
BASE COUNT         4102 a         2808 c         2241 g         4049 t
ORIGIN      
        1 aaacgcgttt ctcgttaata attatttaaa aatacgcatg aagactttct aaaaaacaga
       61 taagtcatca tgcgtatttt tatttgtcag tcaatataag ttgtgctatt ttatctgcct
      121 gtctcagatt ccatggatct gtataaggtc tagctactgc tgcagttagc caatccacac
      181 cttcaacagg tattccatag caataaaagt cagactggat ttcccctgaa gcatcaatca
      241 cgcgattcgt acctcgctca acatctatag cttctgaatg ataacttcct gtattatctg
      301 aaaatgaaaa agggcgaaca agtttgtttt ccttcaaatt ttttatgaca ggattacttg
      361 aacgagaaaa gcttgtttgt ggcaatcgag cttcaatcaa atagcgactg ctgatttctt
      421 tatcttgtag agaaataaca tagcggttgt gcttttgctg aatatcaaat ggcggttcga
      481 tcaaatgaaa aatacccgca tctatcagtg ctgctaattc tttcgtccga atgattggcg
      541 gaccgatcgt taaaaagcta tttagatgag taaaccatcc ccatagtatt tctttgtatt
      601 ccttagcaga aaaaacgtgc cattcaatag cctgccggat tggatcacgc cagtctttta
      661 atgcatcaaa tgttgaagca aatgctcctg tgcaattgcc tttttccgct tcttttatct
      721 gcccttcaat aaatattcgg ctagcttctg aaaagggctc attctgatag gcaagaggag
      781 tttctaaata cgaccaatcc catctataag ggattagtgc tggatgcttt ccgagacact
      841 cctctgaaga gagggagaga aaatcatgct caaaattttt ctttggaatc ggcaagtgat
      901 attcttcgat gattttcttg taataaaaca actcaacgtc ttttttcaga tagtcaaaaa
      961 atgtttcgcc agtcaaaaat cccgactgat gccattgctc caggttttct tttgttagta
     1021 accgtggcca tgccattgct ccgccttgct tttgatttct tcctcgaggg taataaggga
     1081 gcccccttct tgatccactg tatacaatcg gttccttgcc tgatggatga tagaccagtt
     1141 ttcccttttc gttctcgaag cgccctcctc ttgatactgt aaacaatccg acataatcaa
     1201 aaaaacttaa gcctagacct cttaaaaaga cattttcttt aggcggtatc ttcgaaagat
     1261 ctacatctgc aggattagaa ggaagctgat acagtaaaac atttttcttt gcgtagtcta
     1321 cccacactct ttcttcttct gtctcatcat tttcccagtg accgcttgct aaaacaagct
     1381 gatcgataga atagctttgc tgatctgtct gtaaaagata accatctttt cttttgctta
     1441 ctttttttac taaagaataa acaaactgaa tagaagctgg aaattcttct tgcaactttg
     1501 agaaaaacca gcgttgatag agtccgtata aacatcgagt agattgctga tctttttcca
     1561 gacgattgat ttcttctata attagttgct tatttttggt taccgtctgt tcgatataat
     1621 ctgtagcagg tcctttactc cattgaaaca agttgggtcc ctgtgttacc aatcctccac
     1681 tggacaatgt ttcatctgta aaaagcgtca cttgttgaga aacagaattc attaataact
     1741 ccggtggctg attcaaccgc caaactcgac ctccaggacc attgggatcg aaaagaaaaa
     1801 gttcaagaga atgtctgtcc tgattattat ggagaatcct ttctgctaca cttaatcctc
     1861 taggtccggc accaatgatc cctattttca tttgcatgcc tccatttcga taaatttttt
     1921 caataaaaca gcatggttct gatgttcatt ttttgcacca taaacaaaag taatgactgg
     1981 atgttctttc ataagttgac tgagtttaaa aatagcttgc ttcttttctt cttcgaaaat
     2041 caattcttgc tggtatttct ttgcaaatga agcaaatcgt tctggtacat ggtcaaaagt
     2101 tttgcgtgct tcattactag gtgcaatttc tttcaaccac agatctaact gcgctttttc
     2161 ttttgaaatt cctcgtggcc agaatctatc cacgagtatt ctatagccat cctctttttc
     2221 agcatcaagg taaacacgtt ttaatttgat catagttgtt tccttctttc taccttttcg
     2281 cttagcttat atatgtagat aatagcacaa aataaaaaaa atgaacggca caactatcca
     2341 ttgcgccgtt catgtgatct tggtcaaaat ctaatgattg attcggtttt taaagctgtc
     2401 atacaatcat ctgttttata cttttttact tttgattttt ccagaccatc cgtcatatcc
     2461 acccttaagt aaataaatct cttgataacc tgctttacgt aatttatttg ctgtacggat
     2521 gctcaaactt ttcttttgat cgtaaatata gacaggctgg tcttttcgaa tagatcctaa
     2581 tgaatctttc aaaacagtgt aagggatgtt tcttgcacct aaaatatgcc cagcatcaaa
     2641 cacatctttt tcgcgaacat cgattacttg tgcttttctc attccttctc gaaattcttc
     2701 ttctgtcaaa gtcgttgctg aacgtttggc cattactcgt agatagactt cccaaaggat
     2761 gattgcaagc acaatcaata gcaaaatgat attaattacc cataaaacca tgtaatgcct
     2821 ccgttttttt aagcaaattt caaggctaat gaggcagcac caatgacacc agcttcatta
     2881 ccaagctctg ctaatttgat ttttgttgat tctcttactt gcgggaaagt aaattcttta
     2941 aaatatttct ctacacggct acgtaaaaat tctcctgctg ccgatactcc tccacctaac
     3001 acgatacttg acggatttaa tgtattcccc aagtttccgc aagctaaacc taagtagaag
     3061 catacgcgat caacaaccat caatgcaaac ggatcgtctt cttgagccat ttcaaagata
     3121 tctttacttg ttacttcttc tccgttgtct agcattgctt tcagttttga atcccctgcg
     3181 tattcttctg ccaagaaacg acctaggcgt accacacctg tagcgctaga tacagtttct
     3241 aaacatccgc gtttaccgca agtacattcg aacccttcag gatccacagt gatatgtccg
     3301 atttctcctc cagctccgct cacgccgtga atcaaatttc cgccagcaac gatacctccg
     3361 ccaacacctg ttcctagagt aacgaataca acgtctggat cgttttctcc agcacctttc
     3421 catcgttcac ctaaagcagc aacattcgcg tcattatcga tagcgaattt gattcctgtt
     3481 cctgcctcga tcagtttttt cacaaattga acttctgtcc agtttaagtt atacgcacca
     3541 ataaccgttc cagcttcgct gtccacgctt cctggggtac ccatgccgat tccgataaag
     3601 tcttcggctt tcatattata taaattcaaa cgatgattga ttgattcaat gatttcagga
     3661 acgatatgct taccatcatc caaaatattg gtatcgatgc tccatttttg ctgaatctct
     3721 ccttctggtg ttaaaattgc aaattttgct gttgttccac ctaaatcaat cccaataatc
     3781 ttcttgtcca ttacaaattt cctttctttc ggctttcttc aattcggtgc tctcttttta
     3841 atatgcgcca cgctgtcatg tatgtatcat gatcaatcaa acggctatca tacagattac
     3901 gcaattcgat agtcattaat tcaatgtcat agatgcgtgc acccacatag ataaagatgc
     3961 caaatttttt gaacagctgc tgtacatcat ataagctctc catctttaag cacttccttc
     4021 atattataca agtaaaccat atttttttaa acccagtaaa atacatatta caaaaagaaa
     4081 tacaaaaact agtgtagaaa gaatcctttg gtgtatcgaa tagcttgtct gttgtttcgg
     4141 tagacccaaa acattcccta acactaatcc gccaattagc ccaccgacat gtccccaaac
     4201 atctacagag gtatccagaa gaccaaaaac aaatgtcaac acaataaata aaagaaaatg
     4261 tctgactagc tgctggatcg ctgggttatg tttaaaatga aaccctaaga taaacaacgc
     4321 accgaacaaa ccgaatatcg aagtacttgc accaccagac agaacattcg cttcgttaaa
     4381 agcaaagctt gctgtatttc ccattatccc gctgaacata taaatcaaga aaaaacgcca
     4441 atggccatag attgcctcga tttgctgccc cataaaatac agtatcagtg agttcattac
     4501 gaaatgcatc agtccaaaat ggatgaagat tggtgtcact aaacgccacc attcattgaa
     4561 gtgaacaaga taaggaccat acattccggc ataataaggg atctcaagtc gcggaaataa
     4621 ttccatcatg aggaagaccg ctgtctgtat ccctaaaaaa gcataggtcc ataacggtcg
     4681 tcttaaccat gcacgcaatt ttatttgctg ttgataattc atgaaaacct cccatttcat
     4741 tttagtcaat aaacaattgt tggatcttct gatcaaagct ctccggttgc cattgttcat
     4801 tcaattgttc acgaaaaaca aggctacatg tttttttatg aaaattattt aaataccggt
     4861 cgtaataacc accgccaaaa ccgattcgat aaccggctct attgtagata atacctggta
     4921 caatgaggag atcgatcatc tccggcaaat agagcttctc tgattgtggc tcaagaacac
     4981 caaaactcga acgtacaaga acggactgtt catccatttc ataaaacgcc atctgacgtt
     5041 tcggcaaagt tttaggtaca actattttct tttgttcttt ttctgctcgt tggataaccg
     5101 gctgtgtatc cagttcaaat tcattcgata aagtcgtccc tatcactttt gctgacttcc
     5161 agtactcgct ggcgaatagc tgtttataga ttcgctgttc tttttccttt ttcttgccac
     5221 ttttggccag atttttcagc ttttcgattc ctttccggcg taatcttact ttcaattggt
     5281 ccaaaaactc acccttcctt ctctcataaa atggtatagg aaaaaaagaa aaatagcaat
     5341 cgaactttca aataaatgca taaatagaat aaaaagaaga cctagaaatg attggccttc
     5401 cttttttaat gaatttcaac tgttcagcgc gtgtttttgt ctgataaaaa gtgcatacag
     5461 aaaatatggc gcaccaaaaa gggcaatcaa aattcctgta ggtacttccc cacttggtag
     5521 aatcactcta gcaaccatat ctgcactaag cagaaaaatg gaaccgaaca aaccgctcag
     5581 aacaaaggtc cagttatttt ctttacgtac gactagcttc gcagcatgag gagctagtag
     5641 tcctacaaaa gataaactac ctgtcaccgc tacactaccg cttgccaaag caacagctaa
     5701 caacaaaagg attttttgac ttttagccac gttaaggcct aatccttgcg cgcgttcttg
     5761 accaaatgat agcaatacaa tcctctgtgc ataaagtcct cctagcggaa ttaagataac
     5821 cgcccatgga agcaatgccc aaacataagg ccagctcgca ccccagattg atcccgctaa
     5881 ccatgcattc acaaatccat aattttcctt agataattgg atcgtgcata gcaatgttaa
     5941 tgcagaaagg ccggcattaa cggcaatacc ggacaataag attcgattca tacccaaaaa
     6001 gccatttcga tcataggccg caagatagac acagcaagca gctaaaacac cacctagaaa
     6061 ggctacaact agcaatagaa acggcagcga atagtgcgaa aagaaaccta aataaagcat
     6121 cactaaaaat cctgctccgg cattaatgcc caatatacta gaatctgcca attcatttct
     6181 tgttactcct tgcaagatat aaccagaaac gccaagacaa gctcctgcta ataaactgat
     6241 cagtaatcga ggaactcgaa attctctaac aatcagcgcc aaagaagatg attctttccc
     6301 tatgaacgtg gcaaaaagct gatccaggta aataaaaggc gtaccaatca ttaaactacc
     6361 tatagacagg atgacaagta caaaaagcaa gaaatagaac gtccaatata tttttttcac
     6421 cgttgttccc tccttacttg tatcagtaaa aacggaacgc caatcaatgc aatgactaat
     6481 ccaaaaggtg tttcaaaagg gggattgatc atcctcgctg taaaatccgc caacataacc
     6541 aatccaccgc caactaaaaa agagacaggt agaacataac gatagtccgt tccaaccatt
     6601 ccgcgaacaa ctggcggaac caacagccca ataaatgaaa caggtccgac taacgaaacg
     6661 gcaatggctg ctaacactgc aactagacag aaaacaatca accgcgttcg tttaggatga
     6721 cttccaagag ctaatgcatg tgtttcactc aaatgaatca aagatattgc ttttcctaat
     6781 gaaaaagaaa ccaaaacagc aagtatatat aaaggaaaca atactgctaa ttgtttccat
     6841 gtgatattgg cagctccgcc tacaaaccaa aaagcaagat cctgctgcag attaaagaat
     6901 tgggtaagtg cttggctgat agcagtaaaa aaagaactaa tagctacgcc agctaaaatc
     6961 agctgtaaag gattcatgcc cattttcgaa aactgcaaaa aaccaaatac gagcaaagtt
     7021 acagcaaaag aacctgccaa tgacattaga aacacagaaa aatcagaagc tttaggccaa
     7081 aaagcaaaac taagggccat tgccaacgaa gcacccgcgt tgattcctag taagcctgaa
     7141 tctgctaaag gatttcttgt caccccttgc atcaatgcac cgctcaatgc aaaacaactc
     7201 cctacaagaa atgctcccgc cacacgaggc aaacgaatat ttcgtatgat ttggtgactt
     7261 tgttttgatc tatcaaaatc aaacaacgcc tgccaaaaat ctgctttttc cacagtcgct
     7321 gcgccatttg aaagagcaac aaaaaaaata atcaaaagaa caaaacagac caaaagcagg
     7381 agtctatttt tcttcatagt cctcataatt gatcctcctg ctcttttggc aacaaatcat
     7441 aggttagaaa aactggttta tttgttcttg gaagcgtgac aatcgttgca tcaatctcaa
     7501 atattttttt catacactct tttgtgatca cttcttcagg tgtaccttct gtgatcaact
     7561 taccgttttt cattgctaat aaataatctg aaaaacgtga agcatgatta atgtcatgaa
     7621 tcgacatcaa gatcgttgtt tgtttcattt gattgatttc agtcaataac tgtaggatct
     7681 ctaattggtg tgccggatct aaaaatgttg tcggttcatc caagatcaag atatctgttt
     7741 cctgagcaag cgccatagcg atccagacac gctgggtttg tccaccagac agcgttgcga
     7801 tatcccgcat acgaaatcca gctagacctg tagcatccaa cgcccactca attgcttcat
     7861 gatcttgcgt gtccatgcca ctgaacagtt tttgataagg gtatcgtccg taagatacta
     7921 cttcttctac tgtcatacct gtagggtgtt cactagtttg tgctaataat gcaatttttt
     7981 ttgcaagttc ttttgaactg tatgtagaga tatcagagtt attgacaatg atctttcctg
     8041 atttcacagg tagtatacgg gtaaaacttt tcaataaagt agattttcca cttccatttg
     8101 gcccaatcaa actagtgatt ttcccttcag gaatcgtaac agacatgttt tcaatgattt
     8161 ttttgttttc gtagccggct gcaatgtcag ctgctcttaa tctcgtcatt tcacccatcc
     8221 tttcgctgaa aatcattctc aattatgacg ctgtttcaac atttcgtcaa gcgctacttt
     8281 catatctata gccaaatgaa gcggttgaca gcgttcgaaa tatcaggaat aataaccaat
     8341 gagaaagatt ctcaattatg ttaggaggaa agaaatgaaa ttaaaaacaa aagtattcac
     8401 attatctagc atattgctgc tggctggagt actgagcgct tgctccccct cttcagataa
     8461 agaaacagct acaactgcca attcgacgac ttcatccaat agtactcata cagtcacaga
     8521 tacacttgga cataaagtag agattccaaa cgcgcccaaa cggatcatcg gcagttactt
     8581 agaagactat ctgattgcac taggggaaaa acctatcgcc cagtggactg ttggcagtgg
     8641 ttccattcaa gattatctgc aagatgattt aaaggatgtc ccaacgattt cttatgacct
     8701 tccttatgag aaagtgttaa gctttgagcc tgatttgctg ttgatttctt cttccgctac
     8761 tgtcgaaggc gggaaatacg aacaatatag caaaatcgcg ccaacttatg ttgtaaaaaa
     8821 cggaacagat gtcacatggg aagaacaact aaaagatgtc ggcaaagctt tggcaaaaga
     8881 aaaagaggca gaaaaaatca tttccgatta tcaaaaatcc atcaagaaaa ctcgagaaga
     8941 actagcagat aaaatcgaga acaagacagc tgcagtgttg tgggtgacca acaactcagc
     9001 ctttatggtc agcaaaaatc ggtcaagtgg aagaatcgta tacgacgatc tggaatttgg
     9061 tgttcctgat ctagtggaag aggtctccaa aaaagccact tctgactggt ctgctgtttc
     9121 ctctgaaaag ctagctgaat tagatgctga ctacatcatc cttgtgaaca gtgacaaaga
     9181 tgctgccatg ttcaatgaac caagctggaa gaatctaaaa gcagtgacag acaatcatgt
     9241 actgcaattc ggacctgaat ctagttggct atataacgga cctattgcca gtcaggaaat
     9301 ggtcaatgat atcaaagaaa aactaaattg attttaacta aaaaaagcat cttcacgatt
     9361 agtcgtgaga tgcttttttt cttattaaca aactagacgc atagatttta ttcttctttt
     9421 gaagcaaccc tattttttac tctttcccat ttttgaaaac gatgggcata tagattttct
     9481 tgatccgtta tcccctcata ctcctctata agggcgaagt ctgaaaaatc aatattccca
     9541 atatacgtat cgccttcaaa cgtgctatcg atcaatgttc gccagatccg accagtgtaa
     9601 ggaagaagtt cttgaaaaat ccgactacca cccgaaacat agatatcttc tgagatcgtt
     9661 tgactatacg acaaaagttc atcgatcgaa tgcattactt ccgcattttc tttcgtgcga
     9721 aaatttggtt gcgttgtcaa cacgatgata tggcgataag gaagtgaaag atcccccatt
     9781 ccttcatagg tctttcttcc catcactaaa attcgatcca ttgtatgttc gcgaaagaaa
     9841 cgcatatcat ttggcaatcg ccatggtaac aaaccatttt tgccgatcag accattttta
     9901 tcttgtgccc acatggaaat aaacatatta tccgctcctt atgattttag atgttttgag
     9961 ataaaaaaag tctcaattct ttgaccagca aggaattgag aactttaaaa acattatttt
    10021 gtttcacggt gcaaagtaac tttgcgttcg cgtgggcaat atttttgttt ttccaaacga
    10081 tcaggattgt tacgtttgtt tttgcttgtt agatagttac gttctttaca agaagtacac
    10141 tctaaagtga tgtttacgcg catgtttttc cctcccattt ttacaagaat cctctaagat
    10201 gactctcaga cttgattatc ttaccatgat tttttctaga ttgctagtgt ttttcacgat
    10261 tttgttaagt aaaactactt gacgataaat acaacggttt tacttctcaa acaaaatctt
    10321 tttctttttg tataaatacc cttacacttg tttatataga ttatgttttt taataaaggg
    10381 ggatactaat atgccatgga acatgaagga ctttcctgca tcaatgaaaa acttagataa
    10441 attgacaaga aaaaaagcaa tcgacatcgc aaatgcatta ttagatgaag ggtaccctga
    10501 taaccgtgcg atcccaattg ccattgatca agcaaaaaaa tggtatgaag atgctagtga
    10561 atcagaaaaa cgaactttcg aaaaagaaaa aaatccaagc aagacggatg aacacgatac
    10621 gaatcctcgt gctggaaaac tgcttgattc agatgtgatc gtagaatatg aggaagaaca
    10681 atggatcgtg aaatctaaag gggcaaaaaa ggcaagcaac cattttgata caaaaaaaga
    10741 agcaatcgaa aaaggaaagc aagtagcaca aaacaaagaa tcaactttgt tcatttataa
    10801 aaaagatggg acaaaagaaa aagaaatcga ttattgaaca tatgtaaagg ggttgtgacg
    10861 aaaatcgtgt cacaaccccc ttatgttcga aacaatggtg ttcctctgcc aactccgaac
    10921 cataagtcat aatatctata aaacgggaag agctgttttt tgaaattgtt tcttatcact
    10981 cagtgttaaa cggtaggaga aaacctcttt tttattgata tttgattttc atcaaacagt
    11041 cacatagtcg attgcttctg cttgcggaat cgtttcatct acaaagtaga ctttatacca
    11101 tgtcaagaaa ctgaagattg tccagatttt tctttgctct tctttacgtc cttcgtgatg
    11161 gtcatctagc aacttcatga ttttttcttg atcaaaaaat tcttttgcaa aatctgcttc
    11221 gaataatgct ttgacttgtt cgtagccgtg ttcttctttc aaccaagctt tgattggcac
    11281 agggaatcct aatttcacac gattcgacca ttcttccgga agatgtttgt tcgcagcttg
    11341 tctgaaaata tcttttgtgt tgttttgatt caataaaaac ttacttggta tttgttgagc
    11401 caatttcatg acttcgatat ccaataaagg tactcggact tccaaagaac ttgccataga
    11461 aagtttatct gcctttaaca agatatcttt tggcatccat tgatgaagat cgacatattg
    11521 catcttattg acttcatctt tgattccttc tgttttctcg taatgaacag tcatgatttc
    11581 atccacagat ggtgctgttt gatattttgg tgttaacact tctgatgctt ctgattcttc
    11641 gaacactcga gcatgaccga taaaatattc ttttgcagga gcaagtgatt catataaatg
    11701 gatccgtcca tggaagtttt tcatttttcc gattttacgt cctaaattat agcgtgttcc
    11761 ttttggtaat tttttcaatc cttgagcaat cacacggatg aattttgaat tcgtatggaa
    11821 tccatacgtt tggtatcccg caaacagttc atccgcccct tctccagatt ggactacccg
    11881 cacactttgt gaagcaagat ttgctaaaaa gtacaaaggt acgcaagaag gatttgaatc
    11941 aggttcatct aagtgatact ggatcaaagg gaatgctttg aacgacatgt ctccgtcgat
    12001 cactgctgct gtattatcta agtccaatag ttcggttagt tttctggctt caatcgcttc
    12061 attataggtt ttatcatcaa acccgattga gaaagaatga tctggtttca atacagaggt
    12121 tacataactg gaatcaaccc cgctggataa gaaagaacca acttctacat cactgaccgt
    12181 atgtgcttca atagaagctt gaacggtttc gtcgattttt tcgatccatt cttgtcggga
    12241 atgcgtttct tttgtttcaa aatctgcatc ccaatattgc tgaatgtcta tttttccatc
    12301 tttatacgta taataatggc cttcaggcaa acgatagaca tctttgaaga atgtttcgcc
    12361 gtttaatgga gaatattgga aagtcatata aggtttcaac gcttctttgt tcaattcttt
    12421 gttaaaatca ggatgaggca gaaaactttt gatttctgaa ccaaacataa atgttccgtt
    12481 catttctgca taataatatg gtttgatccc aaagtggtct cttgcaccga ataactcatt
    12541 cttttcatta tcccaaatag caaaagcaaa cattccgcgg atcttttgca ataattctgt
    12601 tccccattct tcgtaaccat gcaaaaggac ttctgtatct gcatgtgttt gaaaaacgtg
    12661 gccagctgca atcaattctt ctctcaaagg ctggtaatta tagatttcgc cattaaaaat
    12721 aatgatcttt gtccgatctt cattgtaaat aggctgagta ccgccctcta agtcaataat
    12781 acttaatctt ctgaatccta aagcaacatg tttatcgata tattctcctg aactattagg
    12841 tccacggtga acgattcgat ccatcattgt attaataatt gtttttttgt tgtccttgtc
    12901 gttaacaaaa cctacaatac cgcacattta actttcacat cctaatataa aattactgtt
    12961 tatttcattc atatgcatct tatcatagaa taaatagcgg acgaatattt tctctcttac
    13021 aatttcgtca cgtgaaaaca aaagatgaaa gtttctgttt ttacagaaaa taaagtccca
    13081 atatctaatt taccatgttt tcttttttga ggctacctta ttctcttcgt gttaaaaaaa
    13141 tattagtgga tgctatttcc aaaaaaacca gcaaaaaaag acacgaccta aagatcgtgt
//
LOCUS       DAYYCY010000072        13128 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000072 DAYYCY010000000
VERSION     DAYYCY010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13128
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.072"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            12..84
                     /locus_tag="ACHAZB_001606"
     tRNA            12..84
                     /locus_tag="ACHAZB_001606"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:45..47,aa:Val,seq:tac)
     gene            88..160
                     /locus_tag="ACHAZB_001607"
     tRNA            88..160
                     /locus_tag="ACHAZB_001607"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:121..123,aa:Lys,seq:ttt)
     gene            168..249
                     /locus_tag="ACHAZB_001608"
     tRNA            168..249
                     /locus_tag="ACHAZB_001608"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:202..204,aa:Leu,seq:tag)
     gene            267..339
                     /locus_tag="ACHAZB_001609"
     tRNA            267..339
                     /locus_tag="ACHAZB_001609"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:300..302,aa:Thr,seq:tgt)
     gene            351..422
                     /locus_tag="ACHAZB_001610"
     tRNA            351..422
                     /locus_tag="ACHAZB_001610"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:383..385,aa:Gly,seq:gcc)
     gene            439..524
                     /locus_tag="ACHAZB_001611"
     tRNA            439..524
                     /locus_tag="ACHAZB_001611"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:473..475,aa:Leu,seq:taa)
     gene            535..608
                     /locus_tag="ACHAZB_001612"
     tRNA            535..608
                     /locus_tag="ACHAZB_001612"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:569..571,aa:Arg,seq:acg)
     gene            618..691
                     /locus_tag="ACHAZB_001613"
     tRNA            618..691
                     /locus_tag="ACHAZB_001613"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:652..654,aa:Pro,seq:tgg)
     gene            711..783
                     /locus_tag="ACHAZB_001614"
     tRNA            711..783
                     /locus_tag="ACHAZB_001614"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:744..746,aa:Ala,seq:tgc)
     gene            811..884
                     /locus_tag="ACHAZB_001615"
     tRNA            811..884
                     /locus_tag="ACHAZB_001615"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:845..847,aa:Met,seq:cat)
     gene            898..971
                     /locus_tag="ACHAZB_001616"
     tRNA            898..971
                     /locus_tag="ACHAZB_001616"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:932..934,aa:Ile,seq:cat)
     gene            995..1084
                     /locus_tag="ACHAZB_001617"
     tRNA            995..1084
                     /locus_tag="ACHAZB_001617"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1031..1033,aa:Ser,seq:tga)
     gene            1097..1170
                     /locus_tag="ACHAZB_001618"
     tRNA            1097..1170
                     /locus_tag="ACHAZB_001618"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1131..1133,aa:Met,seq:cat)
     gene            1189..1264
                     /locus_tag="ACHAZB_001619"
     tRNA            1189..1264
                     /locus_tag="ACHAZB_001619"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1222..1224,aa:Asp,seq:gtc)
     gene            1266..1336
                     /locus_tag="ACHAZB_001620"
     tRNA            1266..1336
                     /locus_tag="ACHAZB_001620"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1298..1300,aa:Gly,seq:tcc)
     gene            1350..1423
                     /locus_tag="ACHAZB_001621"
     tRNA            1350..1423
                     /locus_tag="ACHAZB_001621"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1384..1386,aa:Ile,seq:gat)
     gene            1448..1535
                     /locus_tag="ACHAZB_001622"
     tRNA            1448..1535
                     /locus_tag="ACHAZB_001622"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1482..1484,aa:Ser,seq:gct)
     gene            1557..1628
                     /locus_tag="ACHAZB_001623"
     tRNA            1557..1628
                     /locus_tag="ACHAZB_001623"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1590..1592,aa:Glu,seq:ttc)
     gene            1651..1740
                     /locus_tag="ACHAZB_001624"
     tRNA            1651..1740
                     /locus_tag="ACHAZB_001624"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1687..1689,aa:Ser,seq:tga)
     gene            1753..1826
                     /locus_tag="ACHAZB_001625"
     tRNA            1753..1826
                     /locus_tag="ACHAZB_001625"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1787..1789,aa:Met,seq:cat)
     gene            1845..1917
                     /locus_tag="ACHAZB_001626"
     tRNA            1845..1917
                     /locus_tag="ACHAZB_001626"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1878..1880,aa:Asp,seq:gtc)
     gene            1921..1993
                     /locus_tag="ACHAZB_001627"
     tRNA            1921..1993
                     /locus_tag="ACHAZB_001627"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1954..1956,aa:Phe,seq:gaa)
     gene            1998..2068
                     /locus_tag="ACHAZB_001628"
     tRNA            1998..2068
                     /locus_tag="ACHAZB_001628"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2030..2032,aa:Gly,seq:tcc)
     gene            2081..2154
                     /locus_tag="ACHAZB_001629"
     tRNA            2081..2154
                     /locus_tag="ACHAZB_001629"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2115..2117,aa:Ile,seq:gat)
     gene            2166..2237
                     /locus_tag="ACHAZB_001630"
     tRNA            2166..2237
                     /locus_tag="ACHAZB_001630"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2199..2201,aa:Glu,seq:ttc)
     regulatory      2426..2505
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SMK box riboswitch (SAM-III) riboswitch; SAM
                     riboswitches modulate the expression of downstream genes
                     in response to levels of S-adenosylmethionine; SMK box
                     riboswitch shares no known similarity to SAM-I and SAM-II;
                     Derived by automated computational analysis using gene
                     prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            2503..3696
                     /gene="metK"
                     /locus_tag="ACHAZB_001631"
     CDS             2503..3696
                     /gene="metK"
                     /locus_tag="ACHAZB_001631"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFM8614280.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            3826..5298
                     /locus_tag="ACHAZB_001632"
     CDS             3826..5298
                     /locus_tag="ACHAZB_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFM8614281.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            5381..6169
                     /locus_tag="ACHAZB_001633"
     CDS             5381..6169
                     /locus_tag="ACHAZB_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8614282.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(6236..6904)
                     /locus_tag="ACHAZB_001634"
     CDS             complement(6236..6904)
                     /locus_tag="ACHAZB_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HFM8614283.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            7019..8731
                     /locus_tag="ACHAZB_001635"
     CDS             7019..8731
                     /locus_tag="ACHAZB_001635"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8614284.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            8734..10503
                     /locus_tag="ACHAZB_001636"
     CDS             8734..10503
                     /locus_tag="ACHAZB_001636"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010762530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8614285.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFE"
     gene            complement(10708..11274)
                     /locus_tag="ACHAZB_001637"
     CDS             complement(10708..11274)
                     /locus_tag="ACHAZB_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8614286.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(11274..12230)
                     /locus_tag="ACHAZB_001638"
     CDS             complement(11274..12230)
                     /locus_tag="ACHAZB_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFM8614287.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(12230..12886)
                     /locus_tag="ACHAZB_001639"
     CDS             complement(12230..12886)
                     /locus_tag="ACHAZB_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFM8614288.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
BASE COUNT         3823 a         2458 c         2927 g         3920 t
ORIGIN      
        1 aagttatcca tggaggttta gctcagctgg gagagcatct gccttacaag cagagggtca
       61 gcggttcgat cccgttaacc tccatatgag ccgttagctc agttggtaga gcatctgact
      121 tttaatcaga gggtcacagg ttcgagccct gtacggctca tatttttgcg ggtgtggcgg
      181 aattggcaga cgcactagat ttaggatcta gcgccttacg gcgtgggggt tcaagtccct
      241 tcacccgcat aatttaagtt ttacatgccg gcttagctca gttggtagag catctgattt
      301 gtaatcagag ggtcgagggt tcaagtcctt tagccggcat ctttaaacac gcggaaatag
      361 ctcagtggta gagcaccacc ttgccaaggt gggggtcgcg ggttcgaacc ccgttttccg
      421 ctttgccaaa gcaatcgcgc cggggtggcg gaactggcag acgcacagga cttaaaatcc
      481 tgcggtgagt gatcaccgta ccggttcgat tccggtcctc ggcatttcaa atatgcgccc
      541 atagctcaac tggatagagt gtttgactac ggatcaaaag gttaggggtt cgactcctct
      601 tgggcgcgtt aattaaacgg gaagtagctc agcttggtag agcacttggt ttgggaccaa
      661 ggggtcgcag gttcgaatcc tgtcttcccg ataattataa atttaaacac ggggccttag
      721 ctcagctggg agagcgcctg ctttgcacgc aggaggtcag cggttcgatc ccgctaggct
      781 ccatttcatt taaataatga atatcttttt cgcggtgtag ctcagctggc tagagcgtcc
      841 ggttcatacc cgggaggtcg ggggttcgat cccctccgcc gcgattttta agagcctgga
      901 cctttagctc agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc
      961 tacaaggtcc attgtggcgt tgtaagtttc atatggagga ttacccaagt ccggctgaag
     1021 ggaacggtct tgaaaaccgt caggcgggta aaaccgtgca agggttcgaa tcccttatcc
     1081 tcctttttca tattatcgcg ggatggagca gtctggtagc tcgtcgggct cataacccga
     1141 aggtcgtagg ttcaaatcct gctcccgcaa ttaaaaacta aaaacactgg ttccgtggtg
     1201 taggggttaa catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc
     1261 gccatgcggg tgtagtttag tggtaaaacc acagccttcc aagctgttgt cgcgagttcg
     1321 attctcgtca cccgctttta gttttttatg ggcctatagc tcagctggtt agagcgcacg
     1381 cctgataagc gtgaggtcga tggttcgagt ccatttaggc ccattagtac aaaatcttat
     1441 tggttttggg gaagtactca agtggctgaa gaggcgcccc tgctaagggt gtaggtcggg
     1501 aaaccggcgc gagggttcaa atccctcctt ctccgtttca atattttgtt ttacctggcc
     1561 cgttggtcaa gcggttaaga caccgccctt tcacggcggt aacacgggtt cgaatcccgt
     1621 acgggtcatt aatttaatat agttttacat ggaggattac ccaagtccgg ctgaagggaa
     1681 cggtcttgaa aaccgtcagg cgggtaaaac cgtgcaaggg ttcgaatccc ttatcctcct
     1741 ttttcatatt atcgcgggat ggagcagtct ggtagctcgt cgggctcata acccgaaggt
     1801 cgtaggttca aatcctgctc ccgcaattaa aaactaaaaa cattggttcc gtggtgtagg
     1861 ggttaacatg cctgcctgtc acgcaggaga tcgcgggttc gattcccgtc ggaaccgttt
     1921 ggctcggtag ctcagttggt agagcaatgg attgaagctc catgtgtcgg cagttcgatt
     1981 ctgtcccgcg ccattatgcg ggtgtagttt agtggtaaaa ccacagcctt ccaagctgtt
     2041 gtcgcgagtt cgattctcgt cacccgcttt tagtttttat gggcctatag ctcagctggt
     2101 tagagcgcac gcctgataag cgtgaggtcg atggttcgag tccatttagg cccatttatt
     2161 ttattggccc gttggtcaag cggttaagac accgcccttt cacggcggta acacgggttc
     2221 gaatcccgta cgggtcatgg aagatacttt ttgagtatct ttttcttttt atctgaaaga
     2281 atgattttaa aaagcttagc agagttatct ttatggatga ttgtgctaag cttttttgga
     2341 cagttctaat gtaaaatatt tttatatttt ttaagcaaaa aaatgaaaaa atacgagcag
     2401 aaaactgttt tatctgctat acttggttcg ttgcaagttc ccgaaaggat ccgcaggacg
     2461 gagatgcctt gtaaccgaat ttaactaggg ggaatcatta taatggtaga aagacactta
     2521 tttacatcag aatctgtttc tgaaggacat ccagataaaa ttgctgacca aatcagtgat
     2581 gcaatcttgg atgcaatttt aaaacaagat ccaacagcac gagtagcgtg tgaaacgtct
     2641 gtaacaactg gtcttgtttt agtatttgga gaaatatcta cgacggcata tgtagatatt
     2701 caaaaagtcg tacgggaaac aataaaagaa attggttata cacgtgcaaa attcggattt
     2761 gatggagata cagcagctgt attggttgcg attgatgaac aatctcctga tattgctcaa
     2821 ggagttgatg aagcacttga gatccgggat gaagataaaa aagacgtatt agatgagatt
     2881 ggtgctggtg accaaggttt aatgtttggg tttgctgtag atgaaacacc agaattgatg
     2941 cctttaccaa tcgctttgag tcatcgtctg gtacgacgct tggcagattt gcgtaaatca
     3001 aatgaattaa cgtatttacg tccagatgca aaatctcaag taacggttga atatgatgat
     3061 caaggacaac cggaacgcgt agatacaatc gttatttcaa cacagcatga tgatgcagta
     3121 gataatgaaa caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt tattccagct
     3181 gaattattag atgatcaaac gaaatattat atcaatccga ctggccgatt tgtcattggt
     3241 ggtcctcaag gggatgccgg attaacagga agaaaaatca ttgttgatac gtatggcggt
     3301 tatgctcgtc atggtggcgg tgctttttct ggtaaagatg cgacaaaagt tgaccgttct
     3361 gccagctatg ctgcacgtta tattgctaaa aacattgttg cagcagggct tgctcgcaaa
     3421 gcagaagtac agctagctta tgcgattggt gttgctcaac ctgtttcgat ctcaatcaat
     3481 actttcggta ctggaacggt tccggaagaa gaactgattg ctgcagtgag agaaaacttc
     3541 gatcttcggc cagcaggaat catcgagatg cttgatttgc gtcgtccgat ttacaaacaa
     3601 acagctgctt acggtcattt tggtcgtaca gatgtagatt tgccttggga acatacggac
     3661 aaagtagatg cgttgaaagc aagtttagca aaataagtta tattcagctg gaacgatcgc
     3721 caagtggtgg ttgttccagc tttttttatg gcagttaacc tcttgaaaaa gatagtaatt
     3781 atttatctat ttttagatcc tgtcataatg aaagggagga ataagatgaa aaaagaaaca
     3841 aatgttactt tggtcacagt gagtattttc attgcaacgt tcatgacggc tattgaaggt
     3901 acaatcgtaa caaccgctat gccaacgatt gtcggttcat tacatggaat ggaaatcatg
     3961 aactgggtat tttcgattta tttgctgaca aacgctatgc ttactccgat ttacggaaaa
     4021 cttgcggata aagtgggacg gaaaccaatc tttatttttg ggacattatt gtttattatc
     4081 ggttcgttat tttgcggttt atctcaaagc atgattgggt tgattgctgc tcgtgcgatt
     4141 caagggatgg gtgctggggc aatgatgcca gtagctttga cgatcatcgg cgatctttat
     4201 tctattgaaa agcgagcaaa agttttaggt ttgaatagtt cagcctgggg gattgccagt
     4261 gtttttggac cgcttgcggg aggattcatc gttgaaacga tcagctggca ctggatcttt
     4321 tttatcaatg tgccgatcgg aattctatta attattttga tttggctata tttaaatgaa
     4381 ccaaaaatca tacatgagtc taaaccaatg gatatttttg gtagttttag tttgatggcc
     4441 atgcttgtct cattgctgtt agggttccaa ttattaggag acgaaggaat gagtccgctt
     4501 gtttctgctt tgctgctagg tagtatcgta atgttaggta tttttgtttt tgccgaaaaa
     4561 agagctaaag atcctgtcat catgatgcat ttattcaaaa atccactatt tgttgtggtc
     4621 aacttggctg cagcattggc tagtggtttt ttgatgggaa tcgatgttta tatccctatg
     4681 tggatgcaag gtgtattagg gctgaatgca gcaattggtg ggctggtatt agcaccatta
     4741 tcactagtct ggatggtcgg ttctttcctt gcaagcaagt ggatgactca aatgaaaact
     4801 cattgggtct tgagaattgg actactaatc gcagctattg ggggcgcttg gttgtatctt
     4861 atgcctttca gaagtaacta tttatggttt tttgctatat caacggtcat cggtataggc
     4921 ttaggtgtga ccatgactac ctcgactgtc agtgcacaaa atagtgtatc tcgtgaccag
     4981 cttggtgtag caacttcttt taatacgtta gttcgaacaa ttggtcaaac agtcatggtt
     5041 tctatttttg gtttattgat aaatggggta actaaacgag aacttgcagc agcaaattta
     5101 acaaatgatg cagatatcat gaataaactg gtgaatccac aaacggctaa atatattgat
     5161 gcggatattt tgttaccatt aagaaaaatt ctttatgatg ggttacattc cgtttatcta
     5221 gttggcttgt tactcgtgat cattgctttg gtcttaacgc ttttcgttca tgaaaaaagt
     5281 ggtaaaataa aacagtagtt tcggttaaga tatgtacgta gccgaatcat tcaaatttaa
     5341 agttattcaa aaataacttc ttactaagga aggcgaaaat atggcattta ttcaagcaaa
     5401 tatttattcg aatgtgttag aaatggaagt caatctaaat gtgatactac cgcaaacaac
     5461 aaaaaaggtg attggcacca gttcagagca agagacaacg gatatacctg ttttatattt
     5521 acttcatgga atgggtggca atcatagtgt atgggaaaga cgtacgtcga ttgaacgtta
     5581 tgtagcaaac tacggattag ctgtcatcat gccatctact gatcttggct ggtacacaga
     5641 tacagtttat gacatgaagt attggacatt cattgcagaa gagctacctg agatttgtca
     5701 tgaattattc ccgcaattaa caagaaaaag agaaaaaaca tatgccgctg gattatcgat
     5761 gggcggatat ggcgcgttaa aactaggcct tgcaaaatca gaaaatttcg cagcagtaat
     5821 ctctctttca ggcgctgttt ctgtaggaaa tcgaatggat gatcttttga tggttcgtaa
     5881 agcatctttt tgggaaggga tctttggacc attggatcaa atcgaaggtt caaaaaatga
     5941 tccgcaatac ttattgaaag aactagtaaa aagcggaaaa ccagttccaa gattttatat
     6001 ggcttgtggg gaatctgatt ttctttatca ggcaaatcag gaaatggtat ctgctatgca
     6061 ggaacaaggc ttggcggtca cctttgaaca aggacctggc gatcataact ggatcttctg
     6121 ggaccaatgg attcaacgtg cattggcgtg gctaatggaa gaaaaataaa aaaagagtct
     6181 taaaaagata gctcctatag agatcacaaa catagtgatc tctataggag cttttttact
     6241 gctcgttttc tcttctttta ttttgttggc ataagccttc aatataggtt tgtgcactcg
     6301 ttagaaagag tgctttttca ttcaattctg catgggactg catactttct ttaaatccct
     6361 ctaattgatt ttcaactaca taattacgca taaatagaac tgtttctttc aaaaattcat
     6421 ttccgtccat aattttttgt cgaagctgct gttcatgctg ctgatccatt aactgtccaa
     6481 tcaataaatt gtggagagag gcaatggcct gatgactttg ggtgactgaa aatccttcac
     6541 gaacaagtaa ttggatcatt tgctccaaat gttctaatcg actcggaaaa gcgggcacag
     6601 tattgatttc taattctgcc ccacaaggga aacgtgtata aaggtcatag taattttcca
     6661 tatactgcag caactgttca ttccaaggca aagttggatc tggaagcgct aattcttctt
     6721 ccattgtttc agccattatt tgcaagagct gttgtttgtt tttgaaatac caatataaag
     6781 caggtgcttg aacatgaatt ctgtttgcga tgtttcgcat ggaaagctgc gaaagagctt
     6841 gtgtttcagc caggatatca aaggcggcct gaatgatgat tgcttgagaa agctttattt
     6901 ccattttatc aacctcgtgt attgcattat ctttttaaac atggtaaatt gttagtcggt
     6961 ctaattttac agtgttaaat tagtcctgtc aatttaaaca aaggggtgta atcaacgaat
     7021 gaaattaatc ttacgacatg cgaaaaaata taaagtagct gtttttattt ccttgctgtc
     7081 cgtagcagtc atggttgcag cagcattatg gcaacctaag ttactacagc aagtattgga
     7141 agctattatt acagaagata atgatgaaat gaaaacaatt ggtatttacc tgatcagtct
     7201 ggctttgcta ggattagccg caggtgtaac gaacacgatt ttctctgcaa aagtagctca
     7261 aggcgtaagt gcagatatcc gagaagaagc atttcgaaag atccagacat tttcatttgg
     7321 aaatatcgaa cagttctctg ctggaaatct tgttgttcgt ctaacaaatg atatcacgca
     7381 aatccaaaat ttagtgatga tttctttgca atcacttttt cgaattccat tcttatttat
     7441 tggtgccttc attttagcta tgatcacgat gccgcaatta tggtggatca tcgtagcgct
     7501 tatcgttacg gtatttctga ttacggcact ttcatttaca aggatgggca agcacttcat
     7561 gatcattcaa aaactgattg atcgtgtgaa tagcattgca aaagaaaact tactagggat
     7621 tcgtgtagtg aaatcttttg tacaagaaga aaaccaattg aagtcattca gcaaagtaag
     7681 tgaggactta acaaaacaca acatcatagt cggtacatta ttttctgtga tgatcccgtc
     7741 cttcatgctg gctgccaatt tggctgtggt tggggcgatc ttttttgtga gcgatcttgt
     7801 taaagatgat cctacgttaa ttggtggaat tgcttcattc atgaactact tgatgcagat
     7861 tatgatggca atcattattg gcggaatgat gatgatgatg acttcaagag cagccgtatc
     7921 gctgaagcgg attgcggaaa ttttagatgc tgaaccagat cttacttatt tagacgttcc
     7981 agaacaagag ttgacgggtt ctgtcacttt tgaccatgtt tcttttcgtt atcctggtga
     8041 ggatacagat acgcttaaag atatcagctt ttctatcaaa cctggagaaa tggttggtat
     8101 cgtcggggcg actggtgccg ggaagtcaac actagcacaa ttgatccctc gtctatttga
     8161 tccgacagaa ggcaaagttg aagtaggtgg tgtagatctt cgtcaagtaa atgaaaagag
     8221 tttaagacaa acggtttcct ttgttttgca aaaagcaatt ttattctcag gaacgattgc
     8281 tcaaaacttg cgccaaggga aaaagaatgc aacagaaaaa gagatggaac atgcttcttc
     8341 tattgctcaa gcaaaagaat tcatcgaaaa gttatctgat cgttacgaag caccaataga
     8401 agaacgtagc agtaactttt ctggtggtca aaagcaacgt ttatccatta ctcgtggcgt
     8461 tatcggtaat ccaaagatct tgatcctaga tgacagtacg agtgcactgg atgctcgctc
     8521 tgaaaagctg gtccgagaag cactcgatcg agatttgaag ggaacaacga cgattgtcat
     8581 cgcacaaaaa attgcttctg ttgtcaaagc agaccgcatc ttagtattgg atgaaggcag
     8641 attagtcggc gaaggaaccc atgaggaatt ggtagctgga aaccgtgtct atcaggaaat
     8701 ctttgaaact caaaaaggaa cggaggagta agtatgactg atctaataaa agcaagtaaa
     8761 tttttctatc actatttgaa gcggtacaaa ctctcctttt tctttatctt tatcacagtg
     8821 atcattgcta cctatctaca agtaaaggca cctcaataca tcggggaagc ttttcaagaa
     8881 ttagctaatt acgtaggagg cttgatgcaa ggcgtagacg ataaaagcaa atttgtttct
     8941 gtaatatgga aactcttgct gttttatgta ctagctagtg cagccaactt tatctatagt
     9001 attttgttca cacaagtagt agggaagtca acgaaccgaa tgcgtatcgg tctattcaac
     9061 aaattggaaa aattgacgat ccgtttcttt gattctcatc aagacggtga aattttaagt
     9121 cgttttacca gtgacttaga taatattcaa aatagtttga accaagcttt gcttcaagtc
     9181 atcacgaatg ctgttttact tgtcgggatc ttgatcatga tgttccgcca aaatgtagag
     9241 ttagcttggg ctacaattgc ttccactcca gtagccgttt tgatagctgt cttgattatc
     9301 cgcaaagcac ggaaatatgt ggatcttcag caagatgaag tggggaaact aaatggttat
     9361 atggatgaaa aaatcagcgg acaacgtgtc atcatcacaa atggcttaca agaagaaacg
     9421 attgaaggat tcttagagca taatcaaagt gttcgcgaag caaccttcaa aggacaagct
     9481 tattctggtc tactatttcc gatgatgcaa ggaatgtcac tggtcaatac agcgatagtt
     9541 atctttttcg gcggctggtt agcattaaat ggtgatttgg aacgttcggt ggcattaggc
     9601 ttagttgtga cattcgtcca gtattcacaa caatattacc aaccactgat gcagatttca
     9661 tctggataca gcatgatcca attagcgatt acaggggcaa gacgattgaa tgaaatattt
     9721 gatgaaaaag atgaagtcaa accagaaaac tgtaaagcat ttgatggtgt tgaaaaaggt
     9781 cttgcgttgc agcacgtaga ttttggttat tctccagaaa aactagtctt gaaagatgtc
     9841 tcgatcaatg tggacaaagg agaaatggtt gctcttgttg gtccaacagg atcggggaaa
     9901 acgacgatca tgaatctgct aaaccgtttt tacgatgtca atgatggttc agtagtcatc
     9961 gatggaatag atatccgaga ttatgatctt gatgcattac gtagacatgt aggaatcgtt
    10021 ttgcaagaat ctgtattgtt ctccggaacg atcaaagaca atatcacatt cggcaaaccg
    10081 gacgcttcag atgaagaagt catcaatgca gcaaaacaag cgaatattca tgacttcatc
    10141 atgacgttag aaaaaggtta tgatacagag atatccgaag aaaataactt attcagtaca
    10201 ggtcaaaagc agttgatcag tattgctcga acgatcatca caaatccatc tttattgatt
    10261 ttggatgaag caacaagtaa tgtagacacg gtaacagaag caaggatcca aaaagcaatg
    10321 gatgaagcaa tcaaaggaag aaccagcttt gtgattgctc accggctgaa aacaatcttg
    10381 aatgcagata gaatcgttgt cttgcgtgat ggggaagtca ttgaagaagg cagtcatcac
    10441 gagttattag cagaaaatgg attttatgcg gaactttatc ataatcaatt tgtatttgaa
    10501 taaagaggtt gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa tcaaaaacag
    10561 cttctcatgt ttttgatttg gttggactta ttaccgcaga tataagcttt tgagcaccgt
    10621 gaataaagag gttatgaaat caacgctcta acctgagtat tagagaaaaa aacgcaaaac
    10681 atatcattta tgttttgcgt tttttattta tctttttttc tcaatacaat ataaaatagg
    10741 cggctggttc ttttggttga taaaccgata attcagcaca ttatatgttt cctgtggcag
    10801 tttggaacaa aaagaatcaa ccatatccag ttctttttct ccaccttcat gaccatagta
    10861 gacgacaagg atcaatcgtc cgcgaggcac aagtcgtttc aatatttctt ccatagctgt
    10921 gcgtgtagtc tctggcatag tgatgattgc tttatcactt tttgggagat accctagatt
    10981 gaaaatccct gctttgagat tttgttgttc agctatcact tctcctaacg tttcgtggcc
    11041 ttgtagaaac aatgagacac gttcttctaa ctgacgctgt tctagccttt cacgagtagc
    11101 atcgattgct tgttgctgga tatcaaaact atagacatga ccagtttttc cgatgtgttc
    11161 tgctagaaaa accgtatcgt ggccattgcc catcgttgca tctacgacgt gatcccctgg
    11221 ttgcaagatt tcttgcagta atgtatggct gaaatgcaaa gcagtttgca acatcagacg
    11281 ctcacctttt tccgtacatc atattttcct tggaagctat ctctgcgaag aagttcttca
    11341 tcaattgcat tcaagacttc ccattttttc aagctccaca ttggtccaat caaggaatcc
    11401 cacggcgcat cccctgtcaa acgatggatg atgatctctt ttggtatcat ttccagctgg
    11461 tcgcaaatga cattgacata gtcaggacgg ctcattaatt gaaggcgacc ttcatgatag
    11521 tcccgcaaca ttctcgtttt gcgcatcaga tgcatcaaat ggagcttgat tccttggata
    11581 tctgaatcta aaatggtgcg tctgacattt tctttcatca tttctaaact ttcgcctggt
    11641 aatccgttga tcaaatgcgt acaaacacgg atattatgtt tgcgtaattt agccacgcca
    11701 tctaaataag tttgataatc atgtgctcgg ttgatcagtt ttgaagtttc ttcaaaagtt
    11761 gtttgcaatc caagttctac ccataaatac atccgctcgt tcagttcagc aaggtaatca
    11821 actacttcat ctggcagaca atccggtcgt gtaccgactg ataatcctac gacacctggt
    11881 aaattgacta cttgctcaaa tcgttctttg attatttcaa ctggcgcatg ggtgtttgta
    11941 aaattttgga aataaacgat gtattgttgg acatgaggcc atttttgatg catcatgtcg
    12001 atttctttct ggaattggat tggaagaggg tcttcaggtg caacgatcat gtctcccgaa
    12061 ccagatacac tgcaaaaagt acaaccgcca tgggcaacag tcccatctcg attcggacag
    12121 tcaaatcctc catcgatcgg caccttgaaa attttcccgc caaactcatt tcgtaaagcg
    12181 taattccaag aatgatatct tttatttcct tctgaataag gaaaagtcat taaaagtcct
    12241 tctttctgta agagttatct atttgttttc ttttttctct atacactgga taagtcaggc
    12301 acagccaaga aagactgagt aagtaaccgc cgatgatatc agtaggaaaa tgaacgccca
    12361 gataaatgcg gcttatcccg acacttacta taataaacat catcagcaat tgaagcaatg
    12421 tgcgtaaggt tcttttttga acaaaagctg gaagcagaaa tagtaaagaa ccgtaaagaa
    12481 tcatactgcc tgctgcatgt ccactaggga aactgtaact tgtttcaact actaagtgcg
    12541 ccaatgaagg acgctctcgc atgaaaaata gtttgatcac cgggttgatg attccagcaa
    12601 ttacaataaa tccagaaacg aaccaaaatg tttctgccct ttttttctta taccacatcc
    12661 ataaagccgc acaagcaaat aaaatgatga cggtaagagg attaccgaat ttagtcataa
    12721 agagttggta gctgttcaga tcaggataag aagctctgac aatgcctgta aaccactgat
    12781 cgaaaccaga aagccacgac agatggaact ttacgatcca gcccaaaatg aaaaacagga
    12841 caagaaagaa gctaccagcc acctgaaatg atgttttata tttcatacaa ttcctccttt
    12901 gaagcaatga cttattatag catttttggt ccagcgaaaa aaacggtaaa gagataaaac
    12961 caatgtatga ttcctctttg aataaccaat attcttataa aaaaaataac tgtaatcgga
    13021 aagacatttt ttggtaatgc aaatgaaatg ttagggaaat aaaatcagtg tttttcgaaa
    13081 tccttttaaa caagaacgta gacgtaggaa gaacttgaaa atgaacag
//
LOCUS       DAYYCY010000073        12734 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000073 DAYYCY010000000
VERSION     DAYYCY010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.073"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(85..264)
                     /gene="rpmF"
                     /locus_tag="ACHAZB_001640"
     CDS             complement(85..264)
                     /gene="rpmF"
                     /locus_tag="ACHAZB_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFM8614289.1"
                     /translation="MAVLARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(322..891)
                     /locus_tag="ACHAZB_001641"
     CDS             complement(322..891)
                     /locus_tag="ACHAZB_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HFM8614290.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1077..1979
                     /locus_tag="ACHAZB_001642"
     CDS             1077..1979
                     /locus_tag="ACHAZB_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFM8614291.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="ACHAZB_001643"
     CDS             complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="ACHAZB_001643"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFM8614292.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="ACHAZB_001644"
     CDS             complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="ACHAZB_001644"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFM8614293.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="ACHAZB_001645"
     CDS             complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="ACHAZB_001645"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFM8614294.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8547..8732
                     /locus_tag="ACHAZB_001646"
     CDS             8547..8732
                     /locus_tag="ACHAZB_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HFM8614295.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8794..9990)
                     /locus_tag="ACHAZB_001647"
     CDS             complement(8794..9990)
                     /locus_tag="ACHAZB_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFM8614296.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVARAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10160..11596)
                     /locus_tag="ACHAZB_001648"
     CDS             complement(10160..11596)
                     /locus_tag="ACHAZB_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFM8614297.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11889..12698
                     /locus_tag="ACHAZB_001649"
     CDS             11889..12698
                     /locus_tag="ACHAZB_001649"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8614298.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3802 a         2674 c         2250 g         4008 t
ORIGIN      
        1 attttttatt taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt
       61 tttacatttt ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata
      121 gtaaccacaa tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc
      181 gtttaaccct ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga
      241 tgttcttcta gctagtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat
      301 tagacacaat atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag
      361 ttcagatagt tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg
      421 ctggtattct tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc
      481 ttcctcttct tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga
      541 ctctgctaaa ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc
      601 atctctactt tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg
      661 cagttcaaca ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta
      721 atgaacgata tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac
      781 atcaagtatt aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa
      841 agttaacggt gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca
      901 ctcctaagca acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt
      961 gacaccttgc agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat
     1021 catcctcaga atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga
     1081 acacgaaaga tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg
     1141 ctaattattt ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag
     1201 agtttgacac cgtacttatt acaagagatc gatcgcatca cgaattaatc gtcacgcctt
     1261 ctggaaagca gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag
     1321 ctgaaatcac tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca
     1381 atgaatactt tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg
     1441 agattaccga tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt
     1501 tagctgtatt aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt
     1561 tggacaaacg tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg
     1621 tctctttcaa agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc
     1681 ccgcagcatt cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa
     1741 gcaatgactt gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg
     1801 cagagattgc tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc
     1861 agcctcgctt cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag
     1921 aacaagtagt ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa
     1981 agaagagctg tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta
     2041 aacgatgccg cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc
     2101 gcctttagca ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga
     2161 agtcaatccg ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc
     2221 tttgtcagtt gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc
     2281 agcagatgaa gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc
     2341 taataattta gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac
     2401 tggtacacca gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc
     2461 ttttttagct gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg
     2521 gtaaacgccc cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa
     2581 gatatctgca tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc
     2641 agcaacgatt gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt
     2701 ttcagttaca tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc
     2761 attttcagct tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc
     2821 gtttgcagat tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac
     2881 gtcagaagct tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt
     2941 agcagtgata actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat
     3001 tggtactagt tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc
     3061 agaaactttg atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa
     3121 gatttgaaca tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg
     3181 tgctttacgt acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat
     3241 atcgtcagca tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac
     3301 ccctttacgt gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc
     3361 ttcgtctttt tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc
     3421 aacgtgaaca tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac
     3481 atgttcagga tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc
     3541 acgaccaaaa tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt
     3601 atctaaaaga ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat
     3661 acgtgataat tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc
     3721 agattcaatc aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac
     3781 gattttggtt tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta
     3841 cacagcttaa atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat
     3901 atagtgataa gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa
     3961 cgatttcgtt gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga
     4021 cagcgtaagc accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac
     4081 ggacaacgtg tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct
     4141 cagcgaattc atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc
     4201 cgcgatcgcg gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg
     4261 ggatgatgat ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat
     4321 tgcgtcccat tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac
     4381 ggtcaattga ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc
     4441 caggaatatc attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca
     4501 aagccattgc gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga
     4561 attttttcaa ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac
     4621 gtgcagaata caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa
     4681 gacgacgaat atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca
     4741 gtccttcgta aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt
     4801 ctccgccact ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac
     4861 tttatcctaa gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct
     4921 aacattattt ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca
     4981 agtagtattt tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat
     5041 aagttgcccc tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt
     5101 tcgttgcgtt ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat
     5161 cacttgtaat tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca
     5221 agtttgtgaa ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag
     5281 ttgcagttcc tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc
     5341 aacggcaaac aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc
     5401 tccactactg tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc
     5461 tctaacttcc ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat
     5521 cagctgaact tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa
     5581 ataaatacct aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac
     5641 ttcttcttct tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat
     5701 attttggatt tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc
     5761 aaaaactcct actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat
     5821 ccgtatgagg aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat
     5881 atcttctaca aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc
     5941 cttattgacc aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag
     6001 tttattttta cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga
     6061 attcatcagg ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa
     6121 tcccacgaat gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc
     6181 gatatattca tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga
     6241 tacaaaatgc gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg
     6301 cagaatatct gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg
     6361 ttcctgatag acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg
     6421 gtagcgaatg gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg
     6481 gcctggtcga tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct
     6541 tcgtaaaacg ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg
     6601 ttgaaacaag agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata
     6661 cccttctact cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc
     6721 taaaaagtcc attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag
     6781 aaaagcttca tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat
     6841 aactacacct gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt
     6901 tttgaataaa agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt
     6961 tttataagct tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt
     7021 tgcttcactt tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc
     7081 cattgtgcca aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata
     7141 attcaatact tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa
     7201 gcgttcgggg tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt
     7261 gatttctaac acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac
     7321 gattttgttt ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa
     7381 tcccatttga tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata
     7441 gtcagtgatt cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg
     7501 ttttccatta ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga
     7561 aatcgattca gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa
     7621 tcgtttgatc atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc
     7681 agataaattt tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc
     7741 aaaaagttct tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc
     7801 ttttatcaca tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat
     7861 ataaaaattt ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc
     7921 ctctttattg aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc
     7981 ggtttctgtc atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt
     8041 catcagctgc tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc
     8101 ttcataagta taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat
     8161 acgatagaat tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta
     8221 gccttgtttt tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga
     8281 ataagaagta attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt
     8341 taaaattcac tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg
     8401 aagttgatta ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa
     8461 gaactcaaat tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa
     8521 attaagatag aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg
     8581 ttctaggtca agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta
     8641 ctttgacaac aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg
     8701 cgccaagaaa agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc
     8761 aagctctttt ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg
     8821 aaagttgaat aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac
     8881 agcactgtaa ttataagcac gcgcgactgt cgatcctagc atgggaccta aaacttgtcc
     8941 aaagttgcta aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat
     9001 cttactgatc agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa
     9061 gcgaagtata cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa
     9121 tgataaaatg agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc
     9181 tagtttcggt gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac
     9241 aaaaaggata ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct
     9301 gatcgtagtg attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga
     9361 gagatgatcc atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg
     9421 atggaaatct tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc
     9481 tgtgatcaag aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg
     9541 tccgatcaga tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt
     9601 ttctctaggt gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat
     9661 cccattccaa aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc
     9721 cattgttaat gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc
     9781 agctagattt ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc
     9841 tagtcccgaa aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg
     9901 tatgaatggc ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat
     9961 cactaaattt tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat
    10021 acctattttc gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag
    10081 gtcattttat atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt
    10141 tccaatccct tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa
    10201 tatcaacgat ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa
    10261 ttgtatagaa aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa
    10321 agatcgatgc aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa
    10381 aagaagccat catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta
    10441 caatggcgat cagtggagag aataaactac ttaatgcgaa tagcactgcc actactactg
    10501 aagtcaaacc agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg
    10561 tattcgatgt accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt
    10621 tatccatttt tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga
    10681 aaatacctgt gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata
    10741 aactgaatgc gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag
    10801 attgcatgcc ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat
    10861 tacctaacga attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac
    10921 caaggactgt ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta
    10981 aaatggctgt cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg
    11041 ctaacgcagg gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga
    11101 cagagctcga aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc
    11161 cgacatacgc aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa
    11221 tcgcccgaat aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc
    11281 aaataaacac cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa
    11341 aagtgaaaaa tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca
    11401 atcccattac cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga
    11461 agggcatacc tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa
    11521 agaatgtcgt tactcccgcc attatttcag ttgatacatt tgtgttgttt ttctttaaac
    11581 cgaaaaactt ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa
    11641 taataaaaaa taagtaaaat agggcaaatc catcgaacaa tttcaaatta tatagacgtt
    11701 tcattcgctt ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt
    11761 ttagcttatg tttagctatt tatatagcca taactgtagt ttttgttagg aatagctgat
    11821 tgctcttgtt aattacctaa aatatgctag actaaaacaa atgctagttg aggaaggaag
    11881 gacccttatt gaataaaaaa ttaattgcca tcgatctgga cggaactacc ctcaatcaag
    11941 attcgatgat tacatctaaa acaaaagaga ctctaaaaaa agcaatcaat gccggtcatc
    12001 atgtcagtat tgctacagga cgcccattcc gaatgagcca tcagttttac caacaattag
    12061 aattaacaac accgatggtt aattttaacg gcgcattagt acacatccct aatcagcact
    12121 gggatggtga aagagaaacg ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa
    12181 aaaagcagct aaatttagat tttatcgctg ctgaaaatcg tgatactttt tttattgaca
    12241 gttttgattt ctttgatgaa aaaatttttg cttcttctcg tccaggagag aagaatttgc
    12301 tttctcctaa aaatttgacg acaaatccta catctttgct tgtccgaaca gacaaacgtt
    12361 ttgcaggagc tgtatctgct gaattaacac gtcaatttgg cagttatgtc gatgttcgga
    12421 catggggtgg acctactgcc atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag
    12481 gcgtgcaaga gattgctaac tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg
    12541 acgagcataa tgatttggaa ttgttggatt atgcaggttg gggagtagct atggctaacg
    12601 gtactgacca actcaaaggc attgcaaatg atgtcactcc tttgagcaat caggaagacg
    12661 gtttagctgt ttatctggaa aaactactta aactataata gagaattaga ttccacaaaa
    12721 aagaggttgt gaca
//
LOCUS       DAYYCY010000074        12384 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000074 DAYYCY010000000
VERSION     DAYYCY010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12384
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.074"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(47..1156)
                     /gene="rpoD"
                     /locus_tag="ACHAZB_001650"
     CDS             complement(47..1156)
                     /gene="rpoD"
                     /locus_tag="ACHAZB_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFM8614299.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(1180..3021)
                     /gene="dnaG"
                     /locus_tag="ACHAZB_001651"
     CDS             complement(1180..3021)
                     /gene="dnaG"
                     /locus_tag="ACHAZB_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFM8614300.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(3203..5518)
                     /locus_tag="ACHAZB_001652"
     CDS             complement(3203..5518)
                     /locus_tag="ACHAZB_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFM8614301.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(5703..6563)
                     /locus_tag="ACHAZB_001653"
     CDS             complement(5703..6563)
                     /locus_tag="ACHAZB_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HFM8614302.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(6622..7254)
                     /locus_tag="ACHAZB_001654"
                     /pseudo
     CDS             complement(6622..7254)
                     /locus_tag="ACHAZB_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
     gene            7421..8182
                     /locus_tag="ACHAZB_001655"
     CDS             7421..8182
                     /locus_tag="ACHAZB_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8614303.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            8208..10799
                     /gene="mprF"
                     /locus_tag="ACHAZB_001656"
     CDS             8208..10799
                     /gene="mprF"
                     /locus_tag="ACHAZB_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFM8614304.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(10987..12174)
                     /gene="tuf"
                     /locus_tag="ACHAZB_001657"
     CDS             complement(10987..12174)
                     /gene="tuf"
                     /locus_tag="ACHAZB_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFM8614305.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
BASE COUNT         3499 a         2627 c         2194 g         4064 t
ORIGIN      
        1 aaaccaaaca gttttactga tttctcttct ggtcaatagt ccacgcttat tctaagaaat
       61 cttttaattg ttttgaacga gaaggatggc gcaattttct taatgcttta gcctcgatct
      121 gacgaatacg ttcccgagta acaccaaata ctttccctac ttcttccaat gttctcgtcc
      181 ggccatcatc taaaccaaaa cgtaagcgaa ggacattctc ttcccgatca gtcaatgtat
      241 ctagtacatc ttccaattgt tcttttagta actcataagc tgcgtgttct gctggacttg
      301 ttgcttcctg gtcttctatg aaatcaccta agtgagagtc atcttcttca ccgattggcg
      361 tttcaaggga aacaggttct tgcgcgattt tcaagatttc tcggactttt tctgttggaa
      421 gatccatttc cgcaccgatt tcttctggcg taggttctct tccaaggtct tgcagcaatt
      481 gacgttggat acgtatcaat ttattaattg tttcaaccat atgaactgga atacggatcg
      541 tacgtgcttg gtcagcgatt gctcgagtga tggcttgacg aatccaccat gtagcatagg
      601 ttgaaaactt aaatcctttg cggtagtcaa atttttcaac tgccttcatc agtcccatgt
      661 ttccttcttg gatcaagtca aggaactgca tgcctcgtcc tacataacgt ttagcgatac
      721 ttactactaa acgaaggttt gcttctgcca gacgctgttt cgcttcttgg tcgccttctt
      781 cgattttcaa tgctaacgct acttcttctt ccgcagttaa aagggagaca cggccaattt
      841 ctttcagata catacgaacc gggtcattga tttttacacc ggttggcgca gaaagatctt
      901 ctgtttttgc ttgttcagct tttttctcag cactcttcaa gctgtgaatt gatggatcac
      961 cattttcatc caccacactg atacctgcgt cttcaatctt ttggatcaat ttgtccattt
     1021 catctgcatt caaagtgaat ggtgtagcaa gctgattgga taaatcatca tatacaactt
     1081 gtcgtttggg tttgttttct ttgatgaaag ctgctactgc tgcttcatac gtttttttat
     1141 ctgttccatt tgccataaaa gaaggccccc cttgagtagc tatttggctt gttttaactg
     1201 tttcgttaaa ttgatgattt caatcgtcaa ttcaagctca agctgtttgt ttccagttcg
     1261 tctggcttct tgctgcattt gtttttttaa tgttatttca tcagctaagc cagatttgct
     1321 gatcacatct agcaagtctt ggatttctcg tggcgaactt tcttctgcca ccggtaaata
     1381 ttcgatttcc accaccaacc gccttaaatt ttcatcttgc aaaaagtcta aaaactctgc
     1441 aaggttgaat tcataatgtt gtgagacata tgcatcgaat aaaaaataaa cttctgcata
     1501 tgcatcatga ataaactgaa attctcgttc ttttaaaata ttacggacag aaacttcttt
     1561 aaatgcacga tataatagaa tctgctctgc cttttctact tgcgtcaaag gtctcttttt
     1621 ccgtgcagtt tcttgtctcg taacctgagt gtgttgactt tcctgaggtt cttgacgatt
     1681 ttgctgattt gtccttcgaa gatcgcgcag ttgtttttgt atcgtctctc tgccgatctg
     1741 aaactctgtt gacagttgag atagatacat atcttgttcg attggtgaaa caactcggct
     1801 aagttcaact agtaattctt cgaggtattc gatcttgtct ttttcattat ctaaattttt
     1861 cccttgtttt aaataacgtg atttaaaagt aaagactgtt tcgcgcccat gagccactaa
     1921 ttcttggaaa gatgcttctc catacttacg gagatattca tcaggatcaa gcttttctgg
     1981 aacgactacg acagaaagtg ctaatcgact gttttgccga agcaattcaa tccctcgatt
     2041 ggttgcttca aaacctgcgt tgtctccgtc atagcaaaag accaattctt tcgtcagctt
     2101 ttccatttgt tggatctgtt gagctgttag acttgtcccc atagaagcta atccagtttt
     2161 gataccagcc tgccatgcag caatcacatc cataaaccct tcgaacaaca aaatttctcc
     2221 gtttttccgg atatcgcttc ttgctttatc aagattaaat aaaacttgtc ttttattgaa
     2281 cagttctgtt tctggactat tcaagtattt gggctgatct ttttcctgcc ctttttcagg
     2341 tgcaagccag cgtcctgaaa aaccgatcgt ctggccttga gaattacgaa gtggaaacat
     2401 cactctttga taaaaacgat cagacatacg gccatcatca tgcgtgacaa acaatccaga
     2461 gcgttctaac aattcatttg aaattgattc attcttgaat acttgctgaa gaaaagaacg
     2521 ctcattaggt gcaaaaccta tgttgaattc ttcaattaat tctaaagaca acccacgact
     2581 ttgcaaatat tctaaagctg gctgtccagc ttttgtatgc aatagcatgt gatgatagat
     2641 ttccgctgct ttttcatgag cagcaatcag tttccccgta gcagattcag gattttggac
     2701 agctatagta cgctgacgcc attgatcttc taacggtatc tgttcgaagt ctgccacttt
     2761 taccactgct tctggaaaac ttagaccttc tagttcttga agaaaagtaa acacgtttcc
     2821 gcctttacca caaccaaagc aatgaaaaat ctgcttgtct tctgcaacag aaaaagacgg
     2881 tgtacgttct tcatgaaaag gacacagccc caagtaattt ttaccagact ttttcaactg
     2941 gacatattgt ccaatcacct caacgatatt cgtttgactg cgtactgttt cgataacctc
     3001 ttgaggaatc cgttgagcca ttttttcacc ccctgctcta ttgatatctt ttgggtttaa
     3061 cagaagatat ttcaggagaa taaccggtaa aaaatcgcac taactgaaat cagtgcgatt
     3121 ttaaaggaca caattataca tgtttgattg tatcatattt cattgctttt tcaagtataa
     3181 cacatttcta tctcattaag catcaatttg ctactctttt cagtaactga ttttgaatat
     3241 cagccttcca gtacaatact cgaagaacaa tatttacgag catgaataca aagaagtatc
     3301 ctacgctctc tcccaaccct aagtttactt ccaaaattcc atggaacagc attgcagcta
     3361 catatatccc tacgatcgtc gttactgcta ggaagatgcg caatgggttg aagggataac
     3421 aagcttttac aactgccatg caactgatcc caatcagcaa gtaatacatc aaagtagtag
     3481 tttcagcatt tccccatgcg aatgtttttc caagtagata caccgcaatg atattcacta
     3541 ctacaagcaa ggcatttggt aaagcattaa taagtgctgt aggcaaataa cggtaagtga
     3601 tttttctttt atcttgttca aaagataaaa agaaggaagg atacccttca atcgctaaat
     3661 caatcagcgt gatttggata gggataaacg gaaatccaat tgctgtaacg gcacatataa
     3721 tcgataaaat aaatgaataa atcgttttga taaaaaagat gcctgagact ttcgtcacat
     3781 tgttgaccac tcggcgtcct tcaaacagga cttctggcaa tgtagtaaaa tcagagtcca
     3841 acaataccag attcgcaatc tgacgtgttg ctccatcacc ttctgccata gcaatgctgc
     3901 aatctgcttc tcttaacgcc aaaacatcat tcacaccgtc gcctgtcatt gcaaccgttc
     3961 tgccgctttc tttcaattca tttaccagca atttcttttg ctgcggactc actcgtccaa
     4021 aaaccgtata gcgatgagca gcttctcgca cttccatttc ttcagtaatt tgagaaagat
     4081 cgacatacgc atcataattc ggtaatcctg cacgtctagc gatattggag acagtaaaag
     4141 gattgtctcc agaaatcact ttcagatcca caccttcctc atgaagataa gctagtgttt
     4201 cttgagcgtt ttttcggatt ggatcatcga tttcaaacag agcaaccggc tcaacaaatg
     4261 ctggtttttc ttcgttcaat tgctgatttg gagcgatccc caacataagt acgcgatagc
     4321 cattcgcttg ggctgttttt agactgtccg gaagccgatc ttcttccacc aaacgttcag
     4381 gcgctcctac gtacacgact cccagttctg gaaattccat cgcgccccat ttgcgatcag
     4441 aagaaaatgc gactttgttt gtcacatcat accgattaga aagctcaaaa taatctctca
     4501 tagcccgcat agtgatgttg ttatcactgc tttctgccag atacgtcccc ataatttctg
     4561 ggatggcttt ctcgtacagc ggatgtagtg cttctacccg ctggatactc attttccctt
     4621 cagtaatggt ccctgtttta tccaagcata atgtatcgac atgggccagc gtttcaattg
     4681 aatacatgtc ttgaacaagg attcgttttt ttgccagttt ggttacccct gtcgttaatg
     4741 caatacttat tagcaatacc aaaccctttg gaagcatgcc taacaaagct gctgcagatg
     4801 caataacaga aattttgata gaagcatcgc ggatgaacag tgcttccaaa aatagtatga
     4861 tccccaaagg aataatcacg aaactagtga atttggatac tttgcgaatc gaatctacaa
     4921 gttcagaacg aatagctttg tgagtctttg cctcgctagt caagcggaca gcataattat
     4981 ctgcacctac tcggataact tcagccatga cagaaccgct tgtcaaaaag cttccagaaa
     5041 gcaattcagc tccagtttct ttgacaatca aatcagattc gcctgtcaaa agagcttcat
     5101 ttgcttctgc ccgtccagat ataacctgta aatcggctgg tatctgttct cctgctccga
     5161 tttttaccaa atctcctaat accagctctt tagtagaaat cgattgttct ttaccgtccc
     5221 ggatcactag tacttctgat tgggaaacaa tcgataattt ccgtacaaga tttcttgcat
     5281 gtatttcttg ataaattcca ataacgatat tgacgacaat aattgctaaa tacgccatgt
     5341 tggaatacgc acctacaaat aacagacaaa tcccaatacc aaagttcaga aaattgaata
     5401 acgtcaatat attatcgctg aatatttgtt ttgtactttt tgatgcatct tcctggtagt
     5461 cattttgctg gccagaagcc attttctgaa ctacctcttg ctgtgtaagt ccattcattt
     5521 tctttttctc cattttccgc caactttcaa aaataatcac gtgtttaagt ctagcgtgtt
     5581 ccaagattaa aaacaacttg tttttataaa atttatagaa aaacacacga atgattttac
     5641 aaaaaagatg ccaacctttt tgtttgaatc acccgtgtgc tgtttttatt atcaatctat
     5701 ctttacttat attgttcctc caaacggctc atccaccaac ctagtgctgt accggctata
     5761 aaaagaacag gacaagctag taccccagca actcccaatc ccgcgtaatc ggtaggaatg
     5821 atcataatcg ttgaagcaaa aactacacct aaaatgaaat ggaatagctt gctatatgcc
     5881 tttgagaaaa tgtaatccat gatttttgac aatccaagaa cacaaagaat cccaccaatt
     5941 ccgatcggca caatcacttc aagtcgtact tctttaaatc cgtcagccat cgctttatac
     6001 aatcccatat acaccaaaaa attagaaggg ctcaatcctg ggacgatcat acccaaacca
     6061 atcaatccgc cggcaatcag ccatgttcca aaattctgat cgacttgccc gtcaaataat
     6121 tgagcgccgt ataataaaaa caaataagcc agaacaaaag taacaaccat gatgatcaca
     6181 tcagaagtat ctcttccttt tttccctgct tctttccata acgcaggaac agttccagcg
     6241 atacatccga caaaaaacca taatatcgtt gtttcatacg cacctaataa aaaactgact
     6301 aaaaaagaaa gtagaaagat gcctgtcact ccgcctagac caacaggcag aaaaaacaaa
     6361 acattttctt tgaaattttt tgtaaggtgg gctaaaaaag aaatcagccg ctcatacatg
     6421 ccaaagacag ctgctaatgc accaccgctg actcctggca agataaatcc tgaaccgata
     6481 aacattccct tcacaaaccg taacatccag tctttggttg tgtaccccac ttcttgattt
     6541 tccatttttt gccttttttc cactatttgc gcactccttc tttcatttaa ttttattcat
     6601 gattgaacat ttcctgctat tttacttaaa gactttttca taggtctcta ttattttttc
     6661 attcattttg acaatatcag gataaaacat gttacctcca ttatgataga gatagccgcc
     6721 attgtaaagc aaggactgga tccgccaata tcggtattgt ttcccttcct catttcctaa
     6781 aagtgggctc agcacatctt tagaatacgc ttctgctaat tctactgtgt tttctccgcc
     6841 atgatttttt acataggcaa tataatcttt cccaaaatta tatgcttgga cagctgtcca
     6901 taaatcgcat ccttgtttct ttgcttctac tatcatttcc gccaaatatt tgacaccttg
     6961 atcaatactg tcagctggat tatcaatgat ctcagcttct ccatgcagac tttccgagct
     7021 ctgcatgaga tctgtccctt ttccttttga ttcggtcaat attatcgcct tgatcaaatt
     7081 ctcgtagttt tccatatgat aattttttgc agctgttgtt atttctattt gatagctttc
     7141 cactcgtttt accgaacgat aagttcttcc tcataaaaac aacaccgcac caataaccgt
     7201 cagaatgatc atgtaaaaaa gaatacggat aatagataat cgttttcgct tcatttcatc
     7261 acctagttat tttgacatct ttttttatct tactatttct ttatcttctt ggctatttct
     7321 tccttgattt ctgtgtacgc cttaagaaaa aatgaatttt cctgtatttt ttgacatctt
     7381 acgaaaatga tcgttgtttt tcagtaaaaa caattgccca atgactgata taatcgttat
     7441 actttacaca gtaggtgatt atgtgaaaaa agaaaagaaa tatgcaaaaa tgccagatgg
     7501 gagcgaaatt tactatgaaa agagcggtca aggcttccct ctctttttac tgcatggcaa
     7561 tgacggcagc ggccgctttt tttcagaaca ggtccctgta ctcgaacgct actataccgt
     7621 gtatctagta gatagccgag gacatggtcg ttcgacaaac gaagcatcta tgttgaattt
     7681 tcagttaatg gccgaggatc tgaatacgat catgttgctg gaaaaaatcg atcaggcaga
     7741 ttttcttgga tttagtgatg gagcaaatct agcactggta tttgccagca gcttccctaa
     7801 aaaagttcat cgattgattt taaattctgg aaacacgctt gttaaaggcg ttcgtttttc
     7861 tgccagagtc atcagtaata ttcattatgc atgggtatgg ttactctctt tatttcgccc
     7921 aagtcttcgc aaaaatcttt tagttatcaa acttttgctg catgacatcg ggcttacaga
     7981 aaatgattta aaaaaaatca actcccccac tttgatcatc gtgggaaaaa aagatgtaat
     8041 caagctaaaa cattctttgt atatagctaa aactatacca aaagcttcct ttgtgcttgt
     8101 gaaagaacaa ggacatgaat tagctagaaa agatcctgaa cgcttcaatc gagaagttct
     8161 tcaatttctt tctgaaacat aactattagg aatagaaggt gatttatttg ttaaaaaata
     8221 ccatacaatg gctgaaaaac cacctaggac tgttcaaaac aatctttttg atttctgtca
     8281 ttgtcattat tgtcggtgaa ttgatgtcca tcggtaaaac tctatctatc cagcaattag
     8341 gagaaacgtt tgccactata cctgtatgga aaagcggact aatgctattg attggtctta
     8401 tctctgtgtt acctatggtt ggttatgata ttattttgaa tcggatgctt gaacaaaagc
     8461 agaatcctcg ttacctgttt gagacaagct ggctgatcaa tacgatcaac aatattgccg
     8521 gtttcggcgg gtttgttagt gtggggcttc gctctgaact atatggacaa aagaaagaga
     8581 gcagaaacgt gatccaagca ttatcgaaag ttttcttgtt tttgatggct ggactttcta
     8641 tttacagtct gctttctttt ttacttgtag tcttcacgcc agttgctcct tttttgaagc
     8701 aatattggat atggttgatc ggcggcggtc tatattttcc tgtcgtcttt ttatttacta
     8761 ctttcaaaaa gaaaggattc cttggtggta cctcacttaa aactcaagga cagctattgc
     8821 tagtttcatt gttggaatgg accggtgtcc tgtttagttt cctttctgtt ggttatttaa
     8881 tggaaatccg gattgatctg tggcaaacaa ttccattatt cattgctgct tcagtgatag
     8941 gaatcgtgtc tatgattcca ggagaaattg ggagtttcga tgtaatgatg attatcgggt
     9001 tgtccgctat tggtgtgcca agagaaatag tcgttgtgtg gatcttgctc tatcgcctgt
     9061 tctactacat cattccgttt ttgattggaa tcgtcttttt cttcaaaaat atcggttcca
     9121 cttttgatca gcgttattct ggtatcccta aacaattagc aacagaaatt gcgcataaaa
     9181 tcgtggttgt cttattatac ttttctggaa tcatgctagt actttctgcc actatcccgc
     9241 aagcttttac agagtttcgc tggcttcata gtttgaaccc attgaaattt cattttatta
     9301 tccaatttcc tagtatacta ttgggatttc ttttaatcat tatgggcaga gggatcgcag
     9361 cacgtgtgaa acgagcatat ttgcctacaa tcttcttgat tgttctagct ttactttatg
     9421 ttttattaag tgattttagt ttcacaccgg tggttttctt gtctattctg cttctgatca
     9481 ttcttgcttc taaaaacgaa ttatttcggg aacaattgat ctattcatgg gagtggcgta
     9541 cgatcgatgg aatcattatt ggtgcactgt ccttgcttta catcgttatt ggggtataca
     9601 atctgccgga tttccctcat cggcgtcacc attttatttc ctttttctta tttccttctg
     9661 aaaagatttg gttttcggga ttgttggcca tcatcgctgt tagtttcatg atcgttctat
     9721 ttgttcattt ccttcaagga gaaaagaaac agattggcga agcctttaat gaagaaaagg
     9781 ccctgaagat ccttacaact tatggaggaa actccgatag tcaattgatc ttcttaaaag
     9841 ataagcggat gtttgcctat gaaaaagacg gtgaaccaac tgttcttctc caattcgctt
     9901 gcttcaataa taaatgtatt gtaatgggtg atccttctgg taagaaagaa gattttccag
     9961 aagcaatcga agcttttatc gaagaaacag atcgactatg ctatttacca gttttctacg
    10021 aaacaagcga agagattgtt atgattttgc atgaattcgg ctacgatttc attaagatgg
    10081 gggaagaagc ttatgtagac ctgaatagtt ttacaacttc agggaaaaaa atgaagggca
    10141 ctcgtgctgt cttgaatcgg atcgaacgag aaggatttac atttgatgtc ttacaaccac
    10201 cattttctac tgagcaaatg agtactttca aaaatatctc tgataattgg ttgggttcac
    10261 gaaaggaaaa agggttttct ttaggctttt tctctgaaga ttatctacaa agagcgccta
    10321 ttgcagttgt caaaaatacc caagaagaaa ttgtcgcttt tgcaacgatc atgcctactt
    10381 acacgaacaa taaagtagga acgattgatc tgatgcgata tgattctaaa aaagcacctt
    10441 ctggcagtat ggatttcttg ttcttgcatt tgtttgccta tatgaaagaa gaacatatcc
    10501 aatggttcaa tttagggatg gcccctcttt caaatgtcgg aacttccaga aagagttttt
    10561 tacaggaaag gattgcatac ttggtttacg agtttggttc tcatttttat tctttccatg
    10621 gattaagaga atataaaagc aagtacgcta caaactgggt ctcacgttat actctatatt
    10681 ctagagatag ttggattgca tatgttatga ttgctttgct gattattgat aatgcgccag
    10741 ttgagcagac acaaggtaga ataagtggtt tgaaaaaatg gatacgaaga aagtattaaa
    10801 aactgcatac cgatagatga acactctcat gatgaataaa agagactgaa gtaaaacgaa
    10861 agttcatgct tctacaatct cttcttatac aattgaagaa caaagagcgg tccaaacaga
    10921 aatcctgttt ggaccgctct tttattttaa acatttttca ttaccaaaat attcgagtcg
    10981 cgtttcttat gcttcgatct ctgtaacgat acctgaacct actgtccgtc cgccttcacg
    11041 aattgagaat gtcgttccgt tttcaacagc gattggatgg atcaagtcaa catcaatcgt
    11101 cacgttgtca ccaggcatga ccatttctgt atcttctggc aatgtgattg ttcctgtaac
    11161 atcagttgtg cggaagtaga actgtggacg atagttgttg aagaatggag tatgacgtcc
    11221 accttcttct tttgtcaaca cgtacacttc tgctttgaat tttgtatgtg gagtaatgga
    11281 acctggttta gcaagtactt gtccacgttc gatatcgtca cgttggatcc cccgtaacaa
    11341 cacgcctacg ttatccccag cttccccgta atctaacgtt ttacggaaca tttctacacc
    11401 tgttacaact gctttttgtg tttcaggttt gatccctacg atctctacct catcaccgac
    11461 acgaacagca ccacgatcaa tacgacctga ggcaacagtt cctcgacctg taattgagaa
    11521 gacgtcttcc actggcaata gtaatggttt atctgtatca cgttctggtg ttgggatata
    11581 ttcatctact gtatccatca attccataat agcagcttca gcatctggat cgccttgcaa
    11641 tgcttttaat gcggaacctt tgatcacagg agtgtcatcg cctgggaaac catattcgct
    11701 caataactcg cgaacttcca tttctaccaa atcgatcaat tcttcatcat cgactaaatc
    11761 aactttgttc aagaaaacaa tcaggtattt tacaccaact tggcgtgaca ataaaatgtg
    11821 ttcacgtgtt tgaggcatcg gaccgtcagt tgcagaaaca actaagattg caccatccat
    11881 ttgtgctgcc cctgtaatca tgtttttcac atagtccgcg tgtcctggtg catcaatatg
    11941 cgcgtagtgg cgtttttctg tttcatattc tacgtgtgcg gtattgatcg taatcccacg
    12001 ttcacgttct tctggagctg catcaatact agcatagtct tgaggatttg ctaggccttt
    12061 tttgcctaat actgtagtga tcgcagcagt taaagtagtt ttcccatggt caacgtgtcc
    12121 aatcgtacca atgttaacgt gtggtttact tctgtcataa tgttcttttg ccataattta
    12181 caaacctcct aaataatata gatcactgaa aaatttcagc tctcaccttt ttattatagt
    12241 ctttttttga ctaaatcaaa aagaatacgt ccgacttact ttttgtaaga ttcgaactaa
    12301 gcttattata cgtcaatatt ggtcaaagtc aactaagagc actcgtctgt cttatacgcg
    12361 aaaaaagtac cttttataaa taaa
//
LOCUS       DAYYCY010000075        12306 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000075 DAYYCY010000000
VERSION     DAYYCY010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12306
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.075"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            286..693
                     /locus_tag="ACHAZB_001658"
     CDS             286..693
                     /locus_tag="ACHAZB_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8614306.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            707..1108
                     /locus_tag="ACHAZB_001659"
     CDS             707..1108
                     /locus_tag="ACHAZB_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8614307.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1110..1481
                     /locus_tag="ACHAZB_001660"
     CDS             1110..1481
                     /locus_tag="ACHAZB_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8614308.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(1517..1816)
                     /locus_tag="ACHAZB_001661"
     CDS             complement(1517..1816)
                     /locus_tag="ACHAZB_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFM8614309.1"
                     /translation="MLFNETQLWFKFDPTNRFIKDFYKVWNSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(1822..2052)
                     /locus_tag="ACHAZB_001662"
     CDS             complement(1822..2052)
                     /locus_tag="ACHAZB_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8614310.1"
                     /translation="MMEDFIRKNISDEYADFYEQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
     gene            2669..3331
                     /locus_tag="ACHAZB_001663"
     CDS             2669..3331
                     /locus_tag="ACHAZB_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8614311.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            3410..6635
                     /gene="fss3"
                     /locus_tag="ACHAZB_001664"
                     /pseudo
     CDS             3410..6635
                     /gene="fss3"
                     /locus_tag="ACHAZB_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
     gene            6749..7063
                     /locus_tag="ACHAZB_001665"
     CDS             6749..7063
                     /locus_tag="ACHAZB_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFM8614312.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            7076..7450
                     /locus_tag="ACHAZB_001666"
     CDS             7076..7450
                     /locus_tag="ACHAZB_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFM8614313.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            7460..7804
                     /locus_tag="ACHAZB_001667"
     CDS             7460..7804
                     /locus_tag="ACHAZB_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFM8614314.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            7875..9227
                     /locus_tag="ACHAZB_001668"
     CDS             7875..9227
                     /locus_tag="ACHAZB_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFM8614315.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            9287..10429
                     /locus_tag="ACHAZB_001669"
     CDS             9287..10429
                     /locus_tag="ACHAZB_001669"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFM8614316.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            10454..10708
                     /locus_tag="ACHAZB_001670"
     CDS             10454..10708
                     /locus_tag="ACHAZB_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8614317.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            10964..12148
                     /gene="mobT"
                     /locus_tag="ACHAZB_001671"
     CDS             10964..12148
                     /gene="mobT"
                     /locus_tag="ACHAZB_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFM8614318.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            12145..12282
                     /locus_tag="ACHAZB_001672"
     CDS             12145..12282
                     /locus_tag="ACHAZB_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFM8614319.1"
                     /translation="MKRGIVMEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKG
                     K"
BASE COUNT         4168 a         2017 c         2646 g         3475 t
ORIGIN      
        1 gaggggaagt acatatagtt gttgatatag ttgctaatat tgttagctaa tttgttagta
       61 aaactgtata cgaatttaga tagtaaaaaa acagtttgtt ccttatatag aggcactttt
      121 actactgatt aaatgtcaga tatgcttaca aatatactta taaatatgct tacaaaatta
      181 gctaatattt ccgttgacaa agctttgtta ctattgttaa actaaattga ctagcaaaag
      241 tgaaacataa atttattaaa taaatgtaaa aattaggaga aatcaatgtc ttatagagta
      301 caattaataa tttcagaaga tgtagaaagt cagcaatttg gcactaatgt tattaataaa
      361 gtaattaatc cagctctttc tattaatgcg cctttaattc ctactgcttt atcattttct
      421 gtaacagctg tggtgtctga gatagaggat acagaaaaga taaaaatagt agaaattgaa
      481 gttttaaaca agaatgaaaa acaaattttt tctacgggtg aagtatcggt aagcttgcct
      541 cctcaagtta acgatataaa ctttaatata aacgccagaa atgtcttggt tgaagaagca
      601 ggagagcatt atgctgttgt taaatttaat ggaactgaga ttggaaggca aatctttgat
      661 atcaaggtca acaaaccagt ggaaaaaaat taaggagacg atacggatgc ttgatatagt
      721 accatcaaat acacatcgga gtggtagctt aataaatttt gctgcgattt ttgcttgcgc
      781 atcggtaatt ctagcatctc cagttgttgt ggatgcaagt tctactccga aaactaaaaa
      841 tgataatcaa cttgtaatta ctacaaaaaa tgagattaac acaatttcaa gtaatgatgg
      901 caaaattttt gatgtgataa atacggtttt aaaaagaaaa gctgaaatca atataaaata
      961 tgatgaagat ttaaacttgt atttctttgt tatcaaaaca acatctgaat tgttttcctc
     1021 agattacgat gttttggata ctctcgataa tgtattatct gactataaat atatggggaa
     1081 atctgtagta gcaactttgg gggaataaaa tgtttgactt taatgaatat ttatctattt
     1141 ccaaacaaat tgaatcagat gataaatata attctaaaga atcttataga agaactgctg
     1201 tttcaagagc atactacagt gcttttaaaa aatctgatga gtatttgaaa gaaaattatg
     1261 atattattta taatggaagt agcggaaaag gaagccacca aaccgtttgg aatttgtttt
     1321 caacagttaa ggaattgaat accttaggaa tacataatag tgggtataga atgttagaga
     1381 aaagaaaatg tgctgactat attcctaatg aaaccattac gaaaacagat atggcgctga
     1441 tgaatcgaga agcagaaaaa ataataaaca aactcaccta atgaatttca agcgagtttg
     1501 tttgttattt ttttatttaa tctttgtatc tttctggatc aacgaaagta tactttatat
     1561 agtcataacg ccgatgatcg ctacgtgcgt ccggcacgtc agtcacgata tcaaacaaaa
     1621 agtatacacc cttcttcatt ctagttttcg cagcaggaat tttgaaatag ttcttattag
     1681 aatagtagag attgattaat aagctatctt cgattgctaa aaagaaaact tctgaattcc
     1741 atactttata aaaatctttg ataaatctat tcgtaggatc gaatttaaac cataattgtg
     1801 tctcattaaa aagcataacc attactccga tctgttttta aaattagttt ctacctctaa
     1861 tatatatcga gtttttattt tgccttcaga gaatactgtt tctttttttg cagttacagg
     1921 ttgtttattt tcggaaaaag ctaatatagc taaaattgaa acatccatct ggaatttatc
     1981 ttttttgctg ctttgctcat aaaagtctgc atattcatca ctgatatttt ttctaataaa
     2041 gtcttccatc ataaaaatca cctcaaaacg attatacgaa cttacgttct cgttgtaaag
     2101 cgatatttga ggggcaaaaa gggggcaaaa atacgtttat ctttgatata tctcagattg
     2161 aacatagtgt ttattatatg tcggtattgt tgatttatag ctgttttgag ggtattagat
     2221 acataataga atatagtggt tttgtttacg tgcattacac ggaaaagctg ctcgtatcaa
     2281 agaaatcaga agataaacac ggaaaacttc cttggaaatc tgaaatttgt gaaaagtcct
     2341 tgtatatcaa gggctttttc ttttttctct atcacagttt taagccattt ttggctgttt
     2401 tggaccattt ttcaatcata ttcccttaaa aattgtccta gattgtccga cattgactat
     2461 cagtgtcgtc aaaatagggc aaaatttagt agtcagcttg acgacaaata taatacgaaa
     2521 aataccaaaa catttaccga atttagcaac ttttgaaccc aagtttctca aagggtcagc
     2581 tattagcaaa aaagacaaaa catgtacttt tctactaaaa agttgatgtg ataggcttct
     2641 atctaaataa ctattaggag tttttatcgt gaagcaaaat gatggacgta tcatttggaa
     2701 aaatcaaagt gagttaaaac tgattttaac aattaatgaa ttcatcgaaa aacacggaat
     2761 tacatctagc cgtcaatacc agaaaaagct atcagaaaat ccaaattctg caccaagtat
     2821 gtggttcatt aacaaaaaat atggatcatg ggagaattta ttgattagta taggacgaga
     2881 aaatactggg tatggaaaat gggctagaat gtcagaacaa gaattattgg aaatagtaga
     2941 agcctttatt aagtgcgaga agataacctc tcaacgaatg tacgaacaaa aatcagtagg
     3001 gaagaatatt ccttcactta gtaccattaa aaagatgtta ggagatatac gtccactttt
     3061 taaagaaaaa aatgatggat ctcgatttac tgatttcgaa ctgctgttag aactaaaaaa
     3121 tgaaataatc cgattaaaac tacaggatga tttatcaatg acaaagtttc gaaaactagt
     3181 tcaatcccca aaactaccat cagttgatac aattatgaaa agaacgaaca aaaattggga
     3241 ggaattgatg gcagagattg gatttgatta tcgaagaatc aaaatttata agcagagaaa
     3301 caacctatcc aagacaaaga aaactaaata gtatcactct aaatcagacg ttcaaaatta
     3361 aaatttggac gtctttttta ttacccaaaa acgaaaggag atttatttta tgaaaaagcc
     3421 aagatttaag aattggcgtt tgctcgcaac actgagtcta ttatgccaaa caattggtgg
     3481 atcgcttggc ccaacgattg cctttgctga tgaaattact catccacaaa cggtaactgt
     3541 ggaattggat ttggctcacc agtatgctgt cgaaggtaca tttagcgatg gtcgtcccat
     3601 gtcagaagtt accgttccgc actatgcagt ttacaatggt gtgaagcaag atattttctg
     3661 tattgagcca ggggtgccga tttacaatga gttcactcct ggttatgaga aaaatccatt
     3721 gcctgatatg tcggagaaag cgaaattagt ttccgtttta tggaaggaag ctggtacgga
     3781 tgtggataca catattgtgg cccagaagat gatttggcaa gaagttaatg gctacgcttc
     3841 actcaattaa acgttctgat ggtagtgcag taaatatcgc agccattgag tcaaaaatca
     3901 atcaagccat tgcggattat caaaagaaac caagcttcca taactccaca gtgaaaaccg
     3961 tgttaggtca atcgactact gtgacagata cgaacggctt gaatttatcg gagtttgatg
     4021 aagttgtcga gaatatcgca aatattgatt atcgtgtgaa cggcaatcaa ctagtgatta
     4081 ctccaaatgc caattcaaaa gagagtggtg aattaaccct aaagaagtca gctaatacag
     4141 gaacaccggt agcttataaa atggctggtc aacaaacttt gatggcgggg gcaattgata
     4201 agcccaatac ctacacaatc aaaattgatg tggaaactga gggttctttg aagattaaaa
     4261 aagtcgataa agaaacagga aatgttgtac cgggaaccgt tttccattta gactttggaa
     4321 aaacattacc tgcaaaagac gtgaccaccg ataaagaagg cattgcgaca ttggatgaga
     4381 ttcctcatgg cgcgaaagta accattactg aaaaatcagt gccagctcct tatacgattg
     4441 acactacacc aatcaccgca accattaaat ctggtgagac tatttctgtg acttcaaaaa
     4501 atactcgcga aaaaggtcaa atcattttga ataaaatggg ggtggaaaca ggaactgatc
     4561 tttggaacga caaatactca ttagccggaa atacgtttgc cattcgcaaa gacagtccaa
     4621 ccggtgaaat tgtccaagaa atgaccacgg atgaaaaagg tcatgcggaa acaccaaaag
     4681 agattgccaa tgcgttggaa ttgggaacct attatgtgac cgaaaccaaa gctagtaatg
     4741 gctttgtgaa tacctttaaa ccagtaaaag tcgaattgaa ctatgtcaat caaaccgtgg
     4801 ctcttgttac cagtaacgta aaagggcaaa accaagaaat cactggtgaa accactttga
     4861 caaaagaaga caaagatacc ggtaatgaaa ctcaaggtaa agctgagttt aaaggagctg
     4921 aatatactct ctttactgca aaagatggtc aagcggtcaa atggagtgag gcttttaaac
     4981 cagagttagt gaagggaacg aaagcttctg atgaaacggt gactttgact ttagatgaaa
     5041 agaatcaagt tgctgttaaa cacttagcga ttaatgagta ttactggcaa gaaaccaaag
     5101 cacctgaagg atataccttg gatgaaacaa agtaccctgt ttccatcaaa aaggtggatg
     5161 ataacgaaaa aaatgccgtg attacccgag atgttacggc aaaagaacaa gtcattcgct
     5221 ttggctttga tttctttaaa tttgctggat cagctgctgg gactgccgaa actggcttca
     5281 atgatctgac ctttaaagta tcgccattgg aaggaaccag tgagattaca ggtgccgaag
     5341 atgaagcaat tacagcttat aacgagcaac taggttttga tggctatggc aagtttgaaa
     5401 atcttcctta tggggattat ttacttgaag aagtagaggc tccagaagga tttcaaaaaa
     5461 ttacgccatt agaaatccgt tctactttta aagaaaataa agcggattac actaagagtg
     5521 aatacatttt tacgattacc gaaaaaggac aaaaacaacc aattaaaacg gtgaccgttc
     5581 cttacgagaa actgacgaat aaagcatttt ctgttagctt gaatcgtttg atgctctatg
     5641 atttacctga agaagaagat agcttaactt ctcttgggac ttggaaagac ggaggcaaag
     5701 aattgacaga ccttgattct accgagctag ttgataaatt gagctataac ttgcatgaaa
     5761 tcaaagaaga ctggtatgtg gtagcgcaag ccattgatgt ggaagcgaca aaagctgccc
     5821 aagaaaaaga tgaaaaagcc aaaccagtgg taattgccga aacaaccgca acgttggcga
     5881 ataaagagaa aacaggaact tggaaaattt tgcataaatt aaccgcagaa caagttttgg
     5941 ataaaaccat cgtcttattc aactatgtgt atgaaaacaa gaaaccctat gaagcaggtg
     6001 atgatccagt agcgaaagat gctagcttga acaatcaagc ccaaactgtc aattgtacgg
     6061 tggaacgcca tgtttccatc caaacaaaag cccacctaga agatggttcg caaaccttta
     6121 ctcatgggga tgtggtggat atgttcgatg atgtgtcgat tacccatgat gtactggatg
     6181 gctcaaaaga agcttttgaa acaattctgt atgcattact tccggataat acgaccaaag
     6241 aaatttggaa atctggcaaa attgattatg aagtgaatga caaagaattc actaaaaccg
     6301 tacttgcgga aaaagtggac acaagtaaat atccagaagg tacaagtttt accttcaaag
     6361 aaattaacta tggcaaagac ggaaacatca atgggaaaca caatgaggat ctaaaagaaa
     6421 aaactcaaac tttaacacct aaaaaagttc cgacaacacc aagtgcacca gaacaaccgg
     6481 aaataccaac tgttccaagt gactctcaag aatctagtcc tacagtaaag acattcccac
     6541 aaacagggga gaaaaattct aatgttctgc tgtttattgg gtttaccttg atctttgcga
     6601 ctgctgggta ttatttctgg aatcgccgaa actaaggtga tgtgatgaaa caactaaata
     6661 ggaaaaacca acgtgcgcga ccgcctccta aaaaaagtgg tcgcgctatt tttgtatcca
     6721 aaactaaaaa tgaaaagagg aaaagaagat ggaattgaaa tttgttgtgc cagatatggc
     6781 cgaaaccttt ggaaagataa ggtatgccgg cgaaggcgaa gttttaactg aaggatatgg
     6841 gcggaatact acagtaattg gtcgtagtta tcatttgtat tccagtaagc aacgagccga
     6901 tgatattgaa gtggtggtag ctgcggaagc cggcgaaaag gattttgatc aagatcaacc
     6961 actaaaagcc gtgaatcccc atttggttgc caaaggctat gagattgaga atcgtgggtt
     7021 taccgactac gtgttgtatg tcgatgattt agtgaaagca taggaggaaa agaagatgag
     7081 attaacagaa ggtattgttg tagattcagg actaacgttt gggaaattac gattttccgc
     7141 attacgccga gaagtacgga agcaaaatga ggatggaacg gttagtaacg aggtaaaaga
     7201 acggacttat aatttgaaat cctctgcgca aggtcgaatg atccaagtta gtatcccagc
     7261 aaatgtaccc ttacgcgagt ttgcctatga cgcagaggta gagttggtca atccaatcat
     7321 ggatacggtc gccaattatg ttttccgaga aggaactact gtcaattggt tcattaaggc
     7381 cgatgattta gtcttgaaac gacaaccaga tcagggaaat tctacaaacc aaaatgaagg
     7441 aaagaaatag gtgaagcgta tggaaattaa tgttgaacat attttagatt gtctcgatca
     7501 atatggaaaa ggtgagctaa cggaggaaca actcactaaa gcattaacct atgatgaaaa
     7561 aatgtttctg attatgcatc aagggttact ggaagtaggt aacgatgccg aagaagattt
     7621 tgatgtactg aattggttag gggaagaaga gtccttcttt atggttatcg aagtaaatga
     7681 aagtctatgt cgagaagccg aaaccgtatt ggaagaaatt ggtgtggaaa tgccggatgc
     7741 cattgaagtt tttttaaagc aactagtgga aacgaaacaa ctccctatag cagtaaatga
     7801 ctagcataac tgcttgaaat tggatcaatg aaatcctatg aagctttttt cgattggagg
     7861 tggaaaccat aaaaatgtac aaagggcatc gtatacgggc aggagatcag cacttggttt
     7921 atcactttgt tcttggctgg ctactcgctc tgttcatcgg ttggatgagc gtcttttatt
     7981 ttcaagagct tagacaattt gatatttcta agctatcact ttctactata gaaatcgttc
     8041 gttctataaa agatttaatc tatttgctag gaagtctcgt tctttcagga agcacgatgt
     8101 tgctttatat acactttttt caggatcatt ggcgcagttt gtggcatcga caaaagctag
     8161 cccggatgat tctagaaaat cattggtacg aagtaaaaca aactcagagt gaaggatttt
     8221 tcaaagacct caatagtagt cgaaccaaag agacaatcag ttacttcccc aaaatctatt
     8281 accgtatgaa ggatggttta ctttctattc gcgttcaaat ttcactggga aaatatcaag
     8341 atcagctttt aaagctggaa aagaagttag aaagtggtct atattgtgaa ctagtagaaa
     8401 aggaactcaa agactcctat gtggagtaca ctttactgta tgatataatt gccaatcgaa
     8461 ttgggataga cgaagtagtg gcagaaagtg gggctttgcg attgatgaaa aatcaagtat
     8521 gggcttatga ttctttacct catatgttaa tcgctggcgg cacaggtggt gggaagactt
     8581 actttttact cactatcatc gaggctttat taaaatctga tgccgagctt tttgttcttg
     8641 atcccaaaaa tgcggactta gccgatttag gtacggtaat gcctcatgtc tattcccaaa
     8701 aggaggaaat ttctgcttgt gtggaagatt tctatgagcg catgatgact cgtagcaagg
     8761 caatgaaaga aatgcccaac tacaaaacag gagagaatta tgcgtttctt ggacttccac
     8821 caaacttttt aatctttgat gaatacgtgg tttatatgga aatgttaacg acgaaggaaa
     8881 gtgcggtgat tttgaataag ctcaaacaaa tcgtgatgtt aggtcgtcaa tctggtttct
     8941 ttctgattct agcttgtcaa agaccggatg cgaaatattt aggagacggg attcgcgatc
     9001 aatttaattt ccgggtggct ttgggtcgta tgagtgaact gggatattcc atgatgtttg
     9061 gcgaggttga taaaaaattc tttatgaagc gtatcaaagg tcgaggctat gtagatacgg
     9121 gagggagtgt gattagtgag ttttatacgc cacttgtacc aaaaggatat gatttcttaa
     9181 gggaaattag cgacattggt ggattaaacg ttaatacgga aggataaaat aatagtatgt
     9241 agcctaggag tgtcaagaat cctgggcttt ttttacgagg tgatttatga catttttaga
     9301 tttattcgct gggattggtg gcttttgctt aggtatggaa caagcgggtc accagtgtat
     9361 cggcttttgt gaaattgatg attttgctcg ccagagttat aaggcaatcc atgatacaag
     9421 taaggaggtg gaaatgcatg acatcacaag tgtatcagac gagtttattc aatctctcgg
     9481 accagtggat atcctttgcg gtggatttcc gtgccaagct ttttcaattg cgggaaagcg
     9541 gcaaggattt tccgatactc gaggtacttt attctttgaa atcgctcggt tcgccgctat
     9601 tctccaacct aagtttttat tccttgagaa cgtccgggga ttactcaatc acgaaggagg
     9661 ggctacgttc gagacgatcc tccgaacgct ggatggattg gggtatgatg tggaatggca
     9721 agtgcttaac tcaaaggcct acgttcccca aaaccgtgag cggatcttcc ttatcggaca
     9781 ttctcgagac gcatgtaccg aacaagtatt tcctatcatt ggatcgtctc caacatctga
     9841 tcaaaacatc agaaacttat taaatatcaa cccttccaat cgaggaatgg ggggacaagt
     9901 ctatgggtca gatgacgtag cgcctacctt gaccggtgat gaaggaatca aaatcgcatt
     9961 gccggtagat gatggaattt ctgtagcggg tatgttactg ggaaattttg aacaaggcaa
    10021 tcgggtttat gagataactg gaacagcgcc tactttgtca acgaaacaag gcggaacgaa
    10081 gatcatgctt cggaaaaacg aaacagaata tcaagaggtg aagccaggga acagtgtgaa
    10141 cctggctttt cctaattcca caagtcgtcg aggccgtctg ggaaaacaaa gtgtgcatac
    10201 cttattaaca ggagaccaac aagcagtcgt cacggatcag tatcaaattc gaaagttaac
    10261 acctagggag tgctggcggc ttcagggctt tccggactgg gcttttgatc gagcttctca
    10321 agtcaattca gatagtcagt tgtataagca ggctggaaat tctgtaacag ttccggtcat
    10381 ttttgatatt gctagaagat taaaggaggt aaaaacagat gatttatgat gagttgattg
    10441 gggaatctac tgggtgattg gcaagattca gtcagatccg gaattggaag aagaactgta
    10501 ccggctcaat tttgatattc gcaaaaacgg ggtgaaagtc cctgatgatc cgtatgtaat
    10561 tgatgaggaa acggatgctc gaatggagct gaatcaagtc atcagtgaat tggaacgaat
    10621 tgcggacttt gctaaagaac ctgatatccg ccaatacttg tttgagataa aagcagagtt
    10681 ggaaattgag ggtattaccg cggagtaacg gccaaataaa ccactgatag gcactgaccg
    10741 cgggggattt gcgaagcaga aatcctccgt ggtgctgtgc ctgtttatgg acggcgttag
    10801 ccggccatga tgtctcaccc cccgtatcta acaggggggt acaaaaacac aaaaaataag
    10861 caagcaacga taaggaatgg ctgtcctatc aaggataata agcaatttgt gtagatgtta
    10921 actgatttga aaaagttaac accttaaatt tggaggtgag aacttggcac aaaggaattt
    10981 agattatcgg ctattaaaag accggcgtaa tgaatatggc gtttcacaaa ataaattagc
    11041 tacgacttgt ggacttagtc gcccgtactt aaatcagata gaaaatggtg gcgtaaccgc
    11101 atcgacaaaa accatgagga aaatttttga ccaactggaa agtttcaatc ccgagttacc
    11161 tttaacgtta ctgtttgact atgtaaggat tcgctttccc acaacggatg cgaggaaaat
    11221 cattcaagag attctccatt tgaaatttga ttatatgctc cacgaagatt acgcctttta
    11281 ctcctatcaa gaacaatatg ttatgggaga tattgtggtg atgttgtcac atgaagaaga
    11341 taaaggcgtc cttttagaat taaagggtcg tggttgtcgg cagtttgaaa cttttttact
    11401 cgcccaaaag cgtagctggt atgacttttt cgaagattgt ctaaaagccg gtggcgtgat
    11461 gaaacgctta gacctggcaa tcaatgatcg agttggcctg ttagatattc ctgatttaac
    11521 gaaaaaatgt cagaaggaag aatgtatctc cttgtttcgt accttcaaaa gctatcgttc
    11581 cggggaactg ttgaaacctg atgataaaga tggtatgggc aatactttgt atattggcag
    11641 tcttaagagt gaggtctatt tctgtttgta cgaaaaagac tatgagcaat atatcaagtt
    11701 agggattcct ttagaccaga ccgaaacgaa aaatcgtttt gaaatccgac tgaaaaatga
    11761 tcgagcttat catgcaattc aagatttgtt aaaaggtcgc agtattgaaa gtaccacatt
    11821 ttccattatt aaccggtact tgcgatttgc ggataaggtg gaaggcaaaa ggcgaactaa
    11881 ctggcccttg aatgaacaat gggggcgctt cattggacgg aatcagaagg aaattcaatt
    11941 aacttctgaa ccgaagcctt ataccattga acgaacgttg aactggcttg gacgacaagt
    12001 cgcccctacg tggaagatgg ccaaggagtt ggatcggttg aatcagacaa cgtacattca
    12061 ggatatggta aataatactc gactatcaga ccggcacaaa aagattttgg agcaacaaag
    12121 tatggcaatt gaaaatttga ttatatgaaa agaggaatcg tgatggaaat tgtgaaagat
    12181 attttattgg tgtttggtgg cagtatgctt ggtgttactg tgatgtgctt gatgcatgca
    12241 agtgcagcgg cggaccgagc aatggaaaag aaaggaaagt gatgaaatga atttcggtca
    12301 aaattt
//
LOCUS       DAYYCY010000076        12177 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000076 DAYYCY010000000
VERSION     DAYYCY010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12177
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.076"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(139..303)
                     /locus_tag="ACHAZB_001673"
     CDS             complement(139..303)
                     /locus_tag="ACHAZB_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8614320.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            598..1926
                     /locus_tag="ACHAZB_001674"
     CDS             598..1926
                     /locus_tag="ACHAZB_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HFM8614321.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2128..2961
                     /locus_tag="ACHAZB_001675"
     CDS             2128..2961
                     /locus_tag="ACHAZB_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFM8614322.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2977..3714
                     /locus_tag="ACHAZB_001676"
     CDS             2977..3714
                     /locus_tag="ACHAZB_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HFM8614323.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3869..4837)
                     /locus_tag="ACHAZB_001677"
     CDS             complement(3869..4837)
                     /locus_tag="ACHAZB_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFM8614324.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5069..5902
                     /locus_tag="ACHAZB_001678"
     CDS             5069..5902
                     /locus_tag="ACHAZB_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFM8614325.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5936..6775
                     /locus_tag="ACHAZB_001679"
     CDS             5936..6775
                     /locus_tag="ACHAZB_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8614326.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6801..7361
                     /locus_tag="ACHAZB_001680"
     CDS             6801..7361
                     /locus_tag="ACHAZB_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8614327.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7560..9281
                     /locus_tag="ACHAZB_001681"
     CDS             7560..9281
                     /locus_tag="ACHAZB_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HFM8614328.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9446..10588
                     /locus_tag="ACHAZB_001682"
     CDS             9446..10588
                     /locus_tag="ACHAZB_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFM8614329.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10604..11815
                     /gene="thiI"
                     /locus_tag="ACHAZB_001683"
     CDS             10604..11815
                     /gene="thiI"
                     /locus_tag="ACHAZB_001683"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFM8614330.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4151 a         2042 c         2528 g         3456 t
ORIGIN      
        1 gtaaataaag cttatgcaaa gaaaaagaaa tccaatcact tgggtttctt tttcttttat
       61 tattatgatg aatcaagctt tctgtttttg tcacgtatgt tgtaatcttt acgaaagagt
      121 agagttggct actcataatc aaccatactt cccatcatcg ctaaatacga aataaatcag
      181 cattcctaca ccaatcaaag cacaagctgc accaagaaaa gggatgacat tcccaaagtt
      241 atctgtattc ataagtatgc caccacccac aaacaaaaag ccgccagcaa tcaggttttt
      301 cattttattc atctcctttc ttacgttata gtatttcctt ctttcaatga gtattttgaa
      361 agctatctta ttgtatgatg ttcttctagg cctcttcttt attatacagg ccattttcgt
      421 tatctcatag aaatattcct ttttatctga aaactattgt ataccgtatg acatttttct
      481 tcatgaagtt gtaagtatat tctcattcaa atgaaaattt catcatagaa gagattgtat
      541 ttctaaattc ttaagagtaa aatacgaagt ataacttgga tttggaagga tgattggatg
      601 aaagctatta cgcctgatat gacaacaaaa tttaacaaac agtttcaagc aaatatcaaa
      661 cagcaggcat tgcagcgggc agttgtgaag aacggaatta ctgcttctgc tgaaaatgtt
      721 tcagcacatg tggagaatac accggtattt tctatagatt tggctacggg gaaagtggct
      781 aatcaaatgc aatcggggcg ttgctggatg tttgctgcac tgaacacatt ccgtcataag
      841 atgctgaatc aattccattt aaaagagttt gaattatcac aaaattacac ttttttctgg
      901 gataaatatg aaaaggcaaa ttatttctat gaaaacatca ttgcaacagg aaaagaggca
      961 atcgatagcc ggaaagtagc tttcttactc gctactcctc aacaagatgg cggccaatgg
     1021 gatatgatcg tttcattatt ccaaaaatat ggcgttgtgc ctaaatcggt gatgccggaa
     1081 agtgcgaata gttcaaattc tcgagatttg aataactact tgaacaaatt actgcgtaaa
     1141 gatgcagtag tgttacggaa aatggttgct caaggagaaa cattagacgc tattgaagaa
     1201 aagaaagaag aaatgctaga aagcatttat aacttcttag ctatctcatt aggaacacct
     1261 ccaaaagaat tcgattttga gtaccgcgat gaagaaaaga actatcatct tgaccgagga
     1321 ctgacaccac aaatttttta tgataaatac atcggtgtaa aattagatga ttatgttagc
     1381 gtgatcaatg caccaacaaa agacaaacca ttcaatcgtt cttatacagt agagatgcta
     1441 ggaaatgtcg ttggcgggaa agaagtcaaa tatctgaatg ttgatatgga aactttcaaa
     1501 aaattagcga ttgctcagct tgaagaagga gaatctgttt ggtttggttg tgatgtaggt
     1561 caatcttcta ctagaactag tggcatcatg gccttagatg cttattcaat ggatgatcta
     1621 tttgatacgg attttacaat gactaaagct gaacgtttag attttggaga aagtctgatg
     1681 acccatgcga tggtattgac cggtgttgac ttagtagacg ggcaatcaac aaaatggaaa
     1741 gtcgaaaata gctggggcga aaaagttgga aagaatggct tctttgttat gagtgatgcc
     1801 tggatggacg aatatacgta ccaaatcgtt gtaagaaaag atttattgac gaaagagcaa
     1861 ttaaatgcgt tcaatgaaga accaattgtt ctatctccgt gggatccaat gggtgcttta
     1921 gcatagacga gtatttcaat gaagagtatg tagtaaagat aaaaagagct ggcttaagcc
     1981 agctcttttt atctttaaag tccattccct gttttttatg gtccctgtgc tataattcta
     2041 aataaaggat aaggattgga aataatcctt ttatcattaa aaatatgatg acgaagtaga
     2101 ataaaattgg gagtaggggg gaaagtcatg gatatccaaa ctgttttatc tgatctgaaa
     2161 ttaaatggta aagtgattcc agttgttggt ggcgatgtta atcagaccta tcgtataaaa
     2221 accgaacatc gtgcttattt tttaaaaatc catccgaatg taaaaaaagg attttttgaa
     2281 gcagaagtag acggattaaa agaactgtca gcattcgtac gtgtaccgga tacgtatatg
     2341 ctaggtgaaa caagtgaagg cgcttatttg ttaatggaat ggatcgagcc gggaaaaggg
     2401 gatcaacgag atcttgctgc agctttagct aatctccatc agcaaacagc gccacaattt
     2461 ggttttcgta aagataacta tttgggtaca cttgttcaga aaaattcttt tgaagaagat
     2521 tggtggactt ttttctttaa agatcggctt gaatcccaga tttctttggc agaagaaacg
     2581 aatcgatgga atgtacagcg tcaagaaaag tatctgcgat ttaaagaacg tgtattgaaa
     2641 tcggttgaac caaaaaagat cactccacgc ctcttgcacg gagacctgtg gagtggtaat
     2701 gtgttctttg accagcaagg gcatccagtt tttgtcgacc cagctgtttc ttatgggaat
     2761 cgggaacaag atattgcaat gagccagtta ttcggtggtt tccgaccgga atttttagat
     2821 gcttatcaaa ctatatttcc attagaaaag ggatggaaag accgactgcc gatttaccag
     2881 ttatattatc ttttggcaca tttgaatatg tttggagaaa gttacggttc ccaagtagat
     2941 caattactag aaaattttta agaggagggc gtgtttttgg caaaaaaata taccaaaaag
     3001 catgaagttt catattatga gtgtgacatc aaccaaacga tgacatttcc ttcgatgtta
     3061 ggagtagtta tcaaaacgtc tgaagatcaa agcgaccagt tgggaagagg cagtgaattc
     3121 gtcaatagct ttggacttac ttgggtcatc acgaattatt ccatggagat taatcgtctc
     3181 cctagagtcg gtgaagtaat ctcagtaacg actcaagcca tggagtacaa taagtttttc
     3241 tgttatcgga acttctggat ccatgatgaa gatgggaaag aattagtcaa aatagaatct
     3301 gttttcgtat tgatggattt tgtgaatcga aaaatgagca gtgttaatga agagatcatt
     3361 gctccttttg agagtgagaa gatcaagaaa atcaagcgtc aggaaaaaat tgaaaagatt
     3421 gattcgggct tcatgttgcc atatcgtgtt cgtttttatg atatcgatag taatcagcat
     3481 gttaataatg cgatgtattt taattggatc attgacgtat taggatatga ctttttgact
     3541 acgcatgttc cagaaaaagt aattattcga tttgataaag aagtagagta cgggaatgag
     3601 atcgaaagcc attttgaaca aatccctatc gaaaatggtg tcaaaacaaa acatgaaatc
     3661 cgaatgcaag aacagctgta ttgtgaagca aatattatat ggaaaaataa ataaaaaaga
     3721 ggttgtgaca gaagggggca acttcaagaa ataagaagaa atttacgaaa attgtttctc
     3781 aaatttttgt aaattttcga cttagttttc gaagaagcta tttttgtcac agcctctctt
     3841 tttaagttta gctattagat aatgatgttt agttttggat aaacgaaatc aattcttcac
     3901 ccgacaaacc atagtttaca gcaatcaata gttttaaacg tgctttttga ctattaatac
     3961 tgttacagaa aataatcccc attttttcta gttcttttcc cccgccttca taatcataaa
     4021 caggctctgc gattccattg aaacaacgag aaacaagtac tacagggatt ttttggacaa
     4081 gaagcttttc caaagcagct aaggcttgtg gagggagatt ccccgcacct aaagcttcga
     4141 ttaccaaacc gtctactttc gtatgggcga tggcttccaa taagtcccct tgcatcccag
     4201 catatgcttt aacgatcggg atagtcccat ctacagcgga aatgtccagt cgctttgttt
     4261 ctaaaagttc ctgcaagaat aaaattcgat tcttagttac taagccgatg ggacccaatg
     4321 taggtgttcg gaaagtagca acatttgtgg tatgggtctt tgtcacgtag cgtgcagaat
     4381 ggatctcatc gttcatgacc accaaaaccc ccttatctag cgcttcttca caacttgcga
     4441 cacgaatcgc actttcaaaa ttataaaggc cgtcacttcc aagttcatta cttgaacgca
     4501 ttgccccagt caaaacaatt ggtaatttgt tgccaatcgt tgtatcaaga aaaaaagcag
     4561 tttcttctaa tgtatctgta ccgtgtgtga tcaccactcc ggatatacct gatgcataag
     4621 cggacaagat acgttctttt aataccaaca tgtgagaagg catgatgtgt ggtgagggaa
     4681 gattgaagat atcttcaact aataattcta catgcttggg gattgtgatt tctgcatcta
     4741 ataatgggtt tgccacatca ggactaacac cgcccgtgag catgttttct ttcatggcga
     4801 tcgttccgcc ggtgtgtagt acaagtattt ttttcatttt acagacttcc ttacgaataa
     4861 ttttgcatag ccgttaaatc ttattgtata ctatataaaa gagttttgct atcagtgaag
     4921 aataacgaat agcctaactt atcataaaaa agctgacctt attaaaatgg aaaagactca
     4981 accttgacct cctaatacaa tggagtatct ggttgagttt cttttacaag aaagcagcca
     5041 aggaaatcaa tcacgaaggt ggccggaaat gatcagaatc ggattaacat cgtttaaaga
     5101 acatgacaaa ttaactggta aaaagaataa cactctttat gaatatgcag ggtttctacc
     5161 cttagtggaa ttagatactg cctattatgg aattccccga aagtcctcag ttgagaattg
     5221 gataagtgaa gtacctgaat catttcgctt tgtagtcaaa gtctatcaag gaatcagcgg
     5281 tcaaggagac tggaagcctt attatcagac cgaagaagag atgattgcgc attttttaga
     5341 tgttatgtcg ccaatggtag atgctggaaa gatgttttgc tttttagtcc aattcccctc
     5401 atcattcaaa tgtacgaaag aaaatgtcca gtacttgcgc aaacttagaa agtggtttga
     5461 gggttatcct gtttctatag aattgcggga ttattcttgg tacagcgatg aatttcttga
     5521 acgtacaaga aactttatgt ctagagccgc tttttcttta gccatcatcg atgagccaca
     5581 gttgctgaat acaacgattc cttttgatcc atttgtcacc aatccgaact ttgctctttt
     5641 tcgattccat ggtaggaatg cgcaaggatg gcaagcaaat gataaagagt ggcgtaaaaa
     5701 aagaacgtta tatcgttatt cagaaaaaga actgcaagaa ctagcacatg aagtcaaagt
     5761 agttgattct aagacaaaag aggtaggtgt gatttttaat aataattctg gtggtgatgc
     5821 ggctgaaaat gcattagcac tgcaaaaaat attaaatatc aagccagatg gactcaatcc
     5881 aacccaaatg gatctgtttt aagggcttat tttattcaag aaaggggcga agtctatgat
     5941 caaagctatt ttttttgata ttgacggaac attggtcacc aatcgttcaa aagcgttgga
     6001 atcaacaaaa caagcaattg aatatgctcg gaaaaacggc attttatgcg gtgtagcaac
     6061 ggggcgcagc ccagtaaaga tcaaagaaat cattgatgaa ttggaattag atatgtatgt
     6121 cgtgtataat ggtcaactgg tttttactgc agatcgcacg attatcgacc acccttttga
     6181 acaaaaagta ttggaacata ttgttgaatt tgcagatgag aaccatcgac aaatcgtttt
     6241 tggtgcgaga aatcggttag atggatcaac gacgatgcta ttaggacaat cgatttttat
     6301 caagcgacta gtcagctttt taccgcgtaa attcccggta cgtctgatga aaaaaatcct
     6361 ccaggttttt agccctcacc gacaaaagga tcgttatgaa acgttagaga tattaaaaga
     6421 accaatttat caatgtatat tgctcagtcc ggagtcagaa cagaaaaagt tagaacatcg
     6481 ctttcccgag tgtacgtttc agcgttcaaa tacgtatact gtagatatca tcccaaaagg
     6541 tggttcaaaa ttactaggca tccaagcttt cgcaaatgct gtcgaaattg aaatggaaga
     6601 aattatggca ttcggcgatc attacaatga tatcgagatg ctgaaaggtg taggaattgg
     6661 agtggccatg gggaatgctc agatcgaagt aaaacatgct gcagactttg tgactcaatc
     6721 aaatgaagaa gatgggatat acgaaggact gaaacacttc ggtctgattt attagtcaat
     6781 gaaagaagga aaaaatcagt atggaagcag agacaccttt tcaaaaagta gaaacattcc
     6841 acaaaacttt tgatccaaga agaccagaga tacccacggc ttttacagaa aatgaagctc
     6901 atacacgtgc tgcgttcaaa atagaagaac ttgttgaatt tgttgcagct acttcaccta
     6961 ataaagaaca actgcatgaa gctgttatgc tgctgcacga agcattagat aaagcagaag
     7021 aaaaaatact tggtaaaaca cagtggggaa gtgatccgct ggttgaacaa gtagatgcac
     7081 tgacagatat cctttacttt acttatggtt ctttttcatt actaggaata gtgcctgatc
     7141 gaatattcga aatcgtccat caggcaaata tggggaaatt atttccagat ggaaaacctc
     7201 attatgaccc tgtaacaaat aaagtgttga aacctgaaaa ctggcagatg gaatatgctc
     7261 cagaacctaa aatcgcggca gaaatccaac gtcagattca agaagcgatt gctaagaaga
     7321 aatccgctga tataggcatg tgcacaaatg aaaaagagtg atcagagttt cactattttt
     7381 gttatcttta tacgatggaa aaacaaaaga ttttcgttta agttagtgaa tgcatgaaaa
     7441 tagtatgcta taatctagaa tagatgtctt ttgcacgaga gacgtattat ttggatttat
     7501 gccaagcaga acgccatctg ttgcataaaa gatgcatact gaaaacggag gaaccataaa
     7561 tgggaaataa tgttatcatc ggaatcgtca ttgcaatcat tatagtcgcc attgtcgtct
     7621 ttttggttag ttacttgatg aagaaaaaaa accaagatcg attggatgcc ttagaaaaac
     7681 gcaaggaaga attatttgac ttgccagtga ttgaagaagt cgatgatgtt aaaaaaatgc
     7741 atctggttgg gcaaagccaa aatacctttc gtgaatggag tcaaaaatgg aatgacatat
     7801 ctacttcctc gttcgctgaa ctagaaagtc aaattttcga agtggaaaac ctcaatgaaa
     7861 gttttcggtt cttaaaagga aaaaaagcag ttgttgaagc agaagctaca atggaccaaa
     7921 tggagaaaca agtgacggaa atccgtgaag gtctaaagga attgcgggaa acggaagaac
     7981 gcaactcact agctgttcag caggcacttg acgtctatga agaactgaaa gaaaaagtca
     8041 gagaagacga gcatgaatac ggaccagctc tttttgaatt gcaaaaacag atcaaagtga
     8101 tcgaaaaaga atttacgcag tttgtgactt taaacacttc tggagaccct gttgaagccc
     8161 gcgaggtttt ggataaagca gaaaaacata cgtatgaagt ggaagctctg atgaaaaaaa
     8221 ttccaccgct gtatgaggag ctccatacca ctttccctga acaacttgaa gaaatcagtg
     8281 atacttacga gaaattgatg gaagaacaat atgtattccc agaagaagat cttgctgaag
     8341 atatcgcaaa agtaagtcga agaattgaaa attcattagc caatcttgaa aaaacagaag
     8401 ttgaaacggt cgaatttgaa aaccgtgaaa cagctgattt gattgattcg ttatatgata
     8461 tcttagaacg ggaaatggaa gctcagagat atgtaaaaac taaccaatca acgattgcag
     8521 agtatatcaa acatacaaca aagaataacc gtcaattatt gattgagctg gatcacacat
     8581 ctcaaagcta tacattgaac cacaatgaaa ttggccgtgt acgaggtttc caaaccgaag
     8641 tggaagaaat ggaacgtcaa aatgaacaga tgattccaca gatccgtcaa cacgaaattc
     8701 cttattcaga agtgcgcacg ttctataaaa cagtcttcaa agtcttagaa gatatcgaaa
     8761 agcaacaagt agagatcgac gactcattac atgaattgag aaaaggcgaa aaagaagcgc
     8821 aagaaaagat cgatacattc gaattcaaat tgcgcagttt gaaacgtttt gtagaaaaac
     8881 aacgtttgcc tggcttgccg aatgattacc tagaattctt ttttgttgct actgaccgta
     8941 ttgaagaact aagtgttgtc ttgaataaaa ttcgagtaaa catggaagag gtcaatcgtt
     9001 tagttgctct atgtgaagaa gatctagaat tattagataa gaagacacat gatttagtag
     9061 atgcagcagc attaacggag caaatgctcc aatatgcaaa tcgttatcgt catacacacg
     9121 aagaagtgag agaagcaatt gataaaagct actatttatt caataaagaa tatcactatc
     9181 aagaagcatt agacgaaatc ggtacagcat tagagcgagt agaacctggt gctttcaaac
     9241 gcatcgaaga tttttatttt aatcatccag acttagtata aagcaaaaaa gttagtaaac
     9301 cagcaagagc aagacggatt attgtcttgt tcttgtcttt ttttgtatat ttgaaaattg
     9361 aaacaagtga aaatttcacc tgctcttgaa agcaccttgg acaaccgtta taatagaaaa
     9421 gaatttcaaa aaagaggggt ttcttatgat atattttgat aacagtgcaa caaccccaat
     9481 ctttccgcaa agcttagatg catatgtaaa aaccagtcag cggattattg ggaatccatc
     9541 aagcttgcat gatttaggca atcaagctaa ccggttgttg cagcaagcaa gaaagcagat
     9601 cgctgatttg atccatgtag aaccagaaga aatctatttt acttcaggcg gaacagaagg
     9661 agataactgg gtattaaaag gaacgatgat cgaaaaaaga gaatatggga atcatatgat
     9721 catttcagga gtcgaacatc cggccgtcag cgagacagca gaacaattaa aagaattagg
     9781 aatcgaagta agtatcgccc cagtggataa acgcggattt gtacgtgtcg acgaattgaa
     9841 agaattaata agaaaagaga cagtattagt ctctgttatg gctgtgaata atgaagtagg
     9901 atcgattcag cccatccagg aaatcagtga tcttttggat gattttccta aaattcattt
     9961 ccatgtagat gctgttcagg cgattggaaa agtaccattc tcgcaatggt tgacagaaag
    10021 agttgatttt gcaacatttt cagctcataa attccatggc ccacggggaa ctggtttcat
    10081 ttattggaaa aagggtcgtc gtttagcacc attactgaca ggtggagggc aagaaagaaa
    10141 tcagcgaagt gggacagaaa atgttccagg tattgtggct atggttaaag ctttacgttt
    10201 atcattagag ataaaggaac aaaaacctga gcagacacaa cgcttaaaag tagctctaat
    10261 caatgcgttg aagacgtatg agaaagtgac gattttttca gaaggaactg actatgcacc
    10321 gcatatttta tgttttgctc taaaaggaat tcgcggcgaa gttctcgtcc atgccttaga
    10381 agaaaaacaa atatttgttt ctactactag tgcctgctcc tcacgtaaac atatggcaag
    10441 cagtacattg cacgcaatgc atgtaccaaa tgatctggct acatctgctg ttcgaatcag
    10501 cttagatgaa aacaattcgc ttgcagaagt cgagcaattt atgattattt tcaagcagct
    10561 ttataagaaa ttttctgcaa tcaattaaaa gaggagtgtt cttgtgaaat acagtgaaat
    10621 tatggtccgt tatggggaac tttcgacaaa aggaaagaat cgaaaaagct ttatcatgca
    10681 actagcacaa aatgtcagac aagctttgaa agactttcca gaaatcaaaa tccatgctga
    10741 tcgggaccgg atgcatcttt tattgaatgg cgcagacggg cagctagtta ttccaaaact
    10801 agcaaaaata tttgggatcc aaaatttctc tccaagtatt cgtgtagaaa aagatgtcga
    10861 agttttgaaa caagctgttc aagacatcat gaaagaaatt tatactggga aagaaacatt
    10921 taaaattatc gctaaaagaa gcgatcacca atttgaattg acaagtaatg aattgaatca
    10981 aacattaggt aatgctgttt ttgatatttt cccgcatatc caagttcaaa tgcgtcaacc
    11041 agatattcca ttacgtgtag agattcggcg tgatggcgct tatctatcgt atgaaacgat
    11101 caagggagct ggaggattgc ccgtaggaac aagcggaaaa ggaatgctta tgctttctgg
    11161 ggggattgat tctcctgtag cagggtactt tgccatgaag cggggagtgg aaattgaagc
    11221 tgttcatttt gctagtccgc cgtatacaag tgaacaagca ctgcaaaaag caaaagatct
    11281 aacagccaaa ctagcacctt atgtgggaac gatccatttt atcgaagtgc catttaccga
    11341 gattcaagaa gaaatcaaaa agtcagtgcc acaaggatat tggatgacaa ttactcgtcg
    11401 aatgatgttg cgtttgaccg atgaaattcg ccgtattcgt catggtttag tcattttgaa
    11461 tggtgaatca ttaggtcaag tggcttcaca aactttgcaa agtatggtgg ctatcaatga
    11521 agtaacgaat acgccaatca ttcggcctgt agcgacaatg gataaacttg aaatcattga
    11581 agtagcagaa cagatcgata catttaatct ttctatccag ccttttgaag attgctgtac
    11641 gatttttgct cctccacagc caaaaacacg ccctcaactg gaaaaggtcc atcaatatga
    11701 agagcgttta gcaatagatg aaatgatcca acgagcacta gctggcttga agatggaaga
    11761 gatacaagca gaatcgtcta atgaaaaaac tgaagaattt tcagaattgc tataagaggt
    11821 tatgaaaata gtgttttgac cagagtatta gagaaacaaa acgaaaaaat agctccatgt
    11881 attttaccgt ttttgaacta atgtcgaaga ttagctgatt gaataccatt tatcaagaag
    11941 ttatggaaaa gccggttctt ttttactaac tgaaaaataa aattttattt agagatcaag
    12001 ctacagttga taaatgatat aaaaaagaga atttcccatg aaatagcgag gttgtgacag
    12061 aagtgggcag cttcaagaaa taagaaggaa tttacgaaaa ttgcttttca aatttttgtg
    12121 aaatttcggc ttatttttcg aagaagttac ttctggaaca ccgtgtatca gaggttg
//
LOCUS       DAYYCY010000077        12065 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000077 DAYYCY010000000
VERSION     DAYYCY010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.077"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..514)
                     /gene="frr"
                     /locus_tag="ACHAZB_001684"
     CDS             complement(<1..514)
                     /gene="frr"
                     /locus_tag="ACHAZB_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFM8614331.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEV"
     gene            complement(517..1239)
                     /gene="pyrH"
                     /locus_tag="ACHAZB_001685"
     CDS             complement(517..1239)
                     /gene="pyrH"
                     /locus_tag="ACHAZB_001685"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFM8614332.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1375..2256)
                     /gene="tsf"
                     /locus_tag="ACHAZB_001686"
     CDS             complement(1375..2256)
                     /gene="tsf"
                     /locus_tag="ACHAZB_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFM8614333.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2355..3137)
                     /gene="rpsB"
                     /locus_tag="ACHAZB_001687"
     CDS             complement(2355..3137)
                     /gene="rpsB"
                     /locus_tag="ACHAZB_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFM8614334.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3496..3975
                     /locus_tag="ACHAZB_001688"
     CDS             3496..3975
                     /locus_tag="ACHAZB_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFM8614335.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4192..5283
                     /gene="phnW"
                     /locus_tag="ACHAZB_001689"
     CDS             4192..5283
                     /gene="phnW"
                     /locus_tag="ACHAZB_001689"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HFM8614336.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5276..5404
                     /locus_tag="ACHAZB_001690"
     CDS             5276..5404
                     /locus_tag="ACHAZB_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HFM8614337.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            5408..5953
                     /locus_tag="ACHAZB_001691"
     CDS             5408..5953
                     /locus_tag="ACHAZB_001691"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HFM8614338.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6407..8098)
                     /gene="argS"
                     /locus_tag="ACHAZB_001692"
     CDS             complement(6407..8098)
                     /gene="argS"
                     /locus_tag="ACHAZB_001692"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFM8614339.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8518..9465)
                     /gene="arcC"
                     /locus_tag="ACHAZB_001693"
     CDS             complement(8518..9465)
                     /gene="arcC"
                     /locus_tag="ACHAZB_001693"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFM8614340.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9580..10599)
                     /gene="argF"
                     /locus_tag="ACHAZB_001694"
     CDS             complement(9580..10599)
                     /gene="argF"
                     /locus_tag="ACHAZB_001694"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFM8614341.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10690..11919)
                     /gene="arcA"
                     /locus_tag="ACHAZB_001695"
     CDS             complement(10690..11919)
                     /gene="arcA"
                     /locus_tag="ACHAZB_001695"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HFM8614342.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3295 a         2509 c         2189 g         4072 t
ORIGIN      
        1 ctacttcttt gatgctgtta tctgtcaatg tttgtacgtc tttttctaat ccgcgcaaat
       61 catcttcggt aatatcacca tttttttgtt gttttttata ttcatcgatc gcatcacgac
      121 ggatgttacg aatcgcgatt tttgcatttt ctgcttcttt tttaacatct ttagctaatt
      181 cttttctgcg ttcttctgtc agttgcggga taactaagcg aatgacattt ccatcattgg
      241 ttggactgat tccgatatca cttgccataa tagctttttc aacatcttga agaatgctct
      301 tgtcaaaagg tgtgatcatt aaaacacgtg cttctggaat ttggattgat gccatttggt
      361 tcaatggtgt tggcgcaccg tagtagttca cagtaatacg gtccaaaagg ctagcgttcg
      421 cacggcctgc gcggatttgc ccaagttcac gttgaagatt ttgtgctgct tttttcattt
      481 tttcttttgc ttctgtcata attgcatctg ccatttttat ttccccctta cagttgttcc
      541 gatattttcg cctaagattg cacggcgaat atttccgtgt tcatttaaat tgaagacaag
      601 cagtggaata tcattatcca tgcttagtga acttgcagtt gaatccatga cttgtaatcc
      661 tttggcaata acttccagat gagtcagttc ttcaaatttt actgcatttg catctacttt
      721 cggatcagca gaataaacac cgtctacatt gtttttagcc atcaggataa catctgcgcc
      781 gatttctgcc gcgcgtaatg ctgctgttgt gtctgtcgag aagtacgggt tgccggtacc
      841 gcctgcaaag ataacgatcc gtcctttttc taaatgacgt tctgctttac ggcgaatata
      901 tggctctgcg atttgacgca tttcaatcga agtttgcacg cgtgtcggta cacccacgtt
      961 ttccaacgta tcctgtaaag ctaaggcatt cataactgta gctaacatac ccatgtaatc
     1021 agcttgagca cgttccatgc ccatttgtgc tccgatttgt ccgcgccaga tatttccgcc
     1081 gcccacaacg attgccattt caacgcccag ctcgtgaact tctttgattt cttggatgat
     1141 ttctttgatt actggtggtt tgattccaaa tcctgcgtcc ccagctaaag cttctccact
     1201 caattttaag actacacgct gatatttcgg tgtgaccatg ttcattcctc caatattttc
     1261 tattgccatt ttaccatact tagatgattt cgacactcta agactggttt ttcttgtttt
     1321 gcttaaaaaa agggagcgca caaaccgtgc gttcccgtct gttattcaac agaattattt
     1381 tttcacttgg ctcataactt cttctacaaa gttatcttcg cgtttttcga taccttcgcc
     1441 aacttcgaaa cgtacgaatg ttttaacagt tgcgcctttt gatgcaacgt atttttcaac
     1501 agtcatatca ggatctttaa cgaatggttg gtccaccaaa gcgatttctg ctttgaattt
     1561 cttcaagcgt ccttctacca ttttttcaac gatattcgct ggtttgcctt cgttcaatgc
     1621 ttgttctgta agaacagttt tttcatgttc taattcagct tctggaattt gtgattcgtt
     1681 tacataacga ggattgatag ctgctacgtg catagctacg tctcttgcaa ctgtttcgtc
     1741 agttgttcca tctagaacag tcaatacggc aatacgtccg cccatatgca agtatccacc
     1801 aaatgcagca ttgtcgtctt tttctacaac ttcaaagcga cggaagctga ttttttctcc
     1861 gataacttga gttgcttcga tcaagtcaga ttcaattgta cctttatctg ttttgatttt
     1921 cattgcagct tccatgtctg ctggtttgtt ttcagcaaca agttctgcaa tttcttttac
     1981 taaatcttgg aacatttcgt ttttagaaac aaagtctgtt tctgagttta cttcaacgat
     2041 tgctgcagtg ttacctttca cagcaactga agccaatcct tcagctgcaa tgcggtcatt
     2101 tttcttcgca gcctttgcca ttcctttttc acgtaaaaga tcaactgctt tttccatgtc
     2161 gccttctact tcaactaatg cttttttcgc atccatcatt cctacgccag tcatttcgcg
     2221 tagttctttt accattttag ctgtaacgtc tgccatgttt aactcctcct gtgtgttttt
     2281 ctttttaaaa aaagctgctt caaaggagag tgaggcttgt cgcccagcct ttgaaacagc
     2341 tccatcattc aaaattattc tgctgaagaa ttgtcgcctt caacaacatc tacgatttct
     2401 tcgattgatg ttgctgtgtt ttcttctgtg aaatcttctt caactacttg atcttcacct
     2461 tggtttcctt cgatgaaagc atcagccatt tttgaagtaa tcaatttaac ggcacgaatt
     2521 gcatcgtcgt ttgaagggat tactacgtcg atttcatctg gatcgcagtt tgtatcaacc
     2581 atagctacga ttgggatatt taatttgtgt gcttcttgaa cagcaatacg ttctttacgt
     2641 gggtcaacga tgtacattac atctgggatt cttggcatat cagcgatacc gcctaagaat
     2701 ttttcaagac gttcacgttc tttgttcaaa ccagcaactt cttttttagg tagaacttca
     2761 aaagttccat cttcttccat tgcattgatt tgtttcaaac ggctgatacg tttttggata
     2821 gtatcccagt ttgtcaatgt tccgcctaac caacgatggt taacgaagta ttgtcctgca
     2881 cgagttgctt cttctttgat tgcttcttgt gcttgttttt ttgtacctac gaataatgct
     2941 acgccgcctt cttcagcaac gctcttcatg taatcataag ctgcatctac taatttaact
     3001 gttttttgca agtcaatgat gtagattccg tttctttctg tgaagatata tttcttcatt
     3061 tttggattcc agcgacgagt ttggtgacca aagtgtacgc cggcttctag taattgtttc
     3121 atagaaatta ctgccatgtt tgtttcctcc gatttggttt tatttttcct cttcttatct
     3181 tctaccttgc cggcaaactt tttcaagcac ctaccgacaa tccaataaga atgtggattt
     3241 gctgatgcat aaagcaccct cagctattat acagacaaaa gatccgtatc gcaagagcaa
     3301 atatactttt ctgctcaaca ttcaatagtt tacctgaaag ataaaaagaa ttcaagaaaa
     3361 acagtagatt tctctttgtt tctttattat cctttttcca taaaataatt ttaggtataa
     3421 aagacgaata ttattgggca atccttcttt tttttgttat gatacatgtg aaatctttcg
     3481 taaaggagaa agaaaatgcg caaagctcaa agacatttgc tgattaaaca agtcattgaa
     3541 gaattcccga tccgtacgca agaagaacta ttaaacaaac ttgggcatta tggcgttacc
     3601 gccacgcagg caacgatttc tcgagatatc cgtgatctaa aaatcgtgaa ggcacctgat
     3661 gaaaatggcg tttcccgctt cgtattgttt cgcgaaaaag gaactcctga gactaggact
     3721 gaggaagagg atcggctggt tcaaatgatt gaagacattg tattgaaagt cgaacgagtc
     3781 cactttttga cgatcgtcaa taccttgcca gacaatgctc atttatttgc atctgtttta
     3841 gatgagatca aacctccctc tattgtcagt acaattgctg gattcgatac gatcatggtc
     3901 atctcggaaa atgaggagga cgcaaaacga gtagaagaat ttcttcacca tccaactgaa
     3961 gtatcacttt tttaatcaga gcacgtattc ctaatatgga atgcgtgttt ttttattttc
     4021 aatattttcc ttattgaata aacctttaca ctctttacaa aaatacatca ttttttattc
     4081 aaatttttca acaccttgat tgaatcacaa taatcatcat ttatattgga tgttaacaag
     4141 agcagcttct tgctctttgt gtatttcaaa aaagaaatga aaggaaatca tatgtataaa
     4201 ttattaactt ctggaccatt gacgacttct gaaacagtga aaaatgctat gctgatcgat
     4261 cattgtactt gggatcaaga atacaaagaa attacacaat ggataagaaa aaaattattg
     4321 gatcttgctc aagtatcgga aggaatatac acaacggtct taatgcaagg tagcagctcc
     4381 ttcggggtag aatcggtatt aagttctgtg atcaggccag aagatactgt actgatttgt
     4441 tcaaacggcg catatagaca acgaatgatc aacatgtgcg aatgtctgaa actgaactat
     4501 accgtttatg aggtatcgga acatcaaatc ctccaagctc atgtgattac acagctgatt
     4561 gaagagaatc cgtctatctc cgcactagca atggtccata gcgagactac ttccggtatt
     4621 ttgaataatt tggaagagat tgctgctgtc acacaagaaa gagatctttt gtttatagtg
     4681 gacgcgatgt caagctttgg tgggataccg atttgtgtag ccgaactagg gattgatttt
     4741 ttgatttcca gctcaaacaa atgtattcaa ggtgttccag gcttttcttt tgtacttgct
     4801 aataaagcgg aactggaaaa acgaaaaaat gttagccgaa gtttgtcact tgatctatat
     4861 gaccagtacc gcgtgatgga aaacgagaat ggcaaatggc gtttcacttc ccctacccat
     4921 acggttttgg cttttcaaca agcgctgaag gaactagagg cagaaggagg aatcgaaact
     4981 cgatattcac gttatctcgc caataatcat gtgctacgac aaaaaatgac agagatgggg
     5041 ttccagcctt tcattcaaga atatcagggg ccattcatca cctcttttct ttaccctaag
     5101 gactggcgat ttgattttaa tcatttttat cagtatttaa agcaaaatgg atttgtgatc
     5161 tatcccggga aaatcagcga tcatgaatgt tttcgtatcg gaaatattgg cgagatctat
     5221 ccggaagata ttttggaact gacagcaatt atcaaagact ataaggagaa cgttaatgat
     5281 taaaactgta atttttgatt gggcaggaac aactgtggac tttggctgta tggctcctgt
     5341 ccacgctttt agaaatgctt ttctagaaaa aggaatccaa ctgactgaca aagaaatccg
     5401 ctaaccgatg ggaaaattaa aatgggatca tatccaacag cttctttccc tcccttctgt
     5461 caaaaaacaa tggatagcta tttatggaca actacctcag gaaaaagagg tggaggaatt
     5521 gtatcaacaa tttgaacatt atctgtttaa agatttggtc aagcattcta ccttgaaacc
     5581 cgacacattg gaaacgatcg aacggttgag aaacgctggg atcaatattg gttccacgaa
     5641 tggatttaat gctcctatga tgacaatcgt agcagaaacc gcaaaaaaag aaaggtattc
     5701 accagacttc atggcaactt ttgaagacgt cgaagggtat ggtcgaccgt ttccttatat
     5761 gatccataaa aacatgcagt acttcaaaaa caagtcggtt gatgaagtaa tcaaagtcgg
     5821 agatacggtc tcagatatcc aggaagggaa aaatgccgga gtgttgacag taggtgtaat
     5881 tgaaggtagt tcgctaatgg ggatttctta tgatgagtac caaaaattag agatggggaa
     5941 aaagatcgtt taaaacagtc tgttgctctt agattccaac aagccggcgc tgatatttgt
     6001 gtaaacaatt taaaagagtt aacagaatac attttgtacg ctgacatgaa tccagaatga
     6061 cttttataca aaataagagg ttgtgaaaaa gctgtcctgt atcaagtaat aaaaacagcc
     6121 aagcaaaaat agctcctcat atttttcgtt tggttaggct tattaccgca gatgcaagct
     6181 tttgaacacc gtttattcga aaaaaaaaag ggagctgtga cacgactttt gtcacagtcc
     6241 ccttatttgc aaacaaacag tgtttaagag acaacctctc cactaaacgg tgttcattca
     6301 gctgactctc ggtatttgct caaaaaacga taaaatttga aaagctattt tctcttttgt
     6361 ttctctaata ctcggatcag aacgctgatt tcacattctc tttttcttac atcttatctg
     6421 gtgcatgaag tcctagcaaa cgaaggtctt ctttcaataa tacagtcact gcataaacta
     6481 gagctaagcg tgcttctttt tgttcatcat cagctaaaat cttcgtatga gcatagtatt
     6541 tgttgaatgc ttgtgctaat ttgattgcat gtttcgcaat cactgacggt tcgtattttt
     6601 ccccagcaga caagaccgtt tcagggtatt tttgaactaa tttaaccact tcccagctgg
     6661 tatcgtcatt caatgcatag tctgctgttt ctgaagggac aaatcctgct ttttccaaca
     6721 ggctgactgc acgagcatgt gtatattgca cataaggtcc agtttctcct tcaaatcgaa
     6781 caacttcttc caggttaaag tcaaatgtat tcagtcgatc gtttttcaaa tcgtggaaaa
     6841 tgactgcccc aaccccaact tgtttagcta cagcatcttt gttttccaaa tcagggtttt
     6901 tctcgctgat ttgttcttta gcagattgga ttgcttcatt taatacttct tctaataaga
     6961 cgatctttcc tttacgagtt gataattttt taccaccttg cgtgatcaaa ccaaatggaa
     7021 tatggtgcat gtcgtcagac cagtcaaagc ccatttcctt caagacagct ttcaactgtt
     7081 taaaatgata gctttgttca ttccctacta catatagaga ttgtttgaag tcgtaagtac
     7141 gtttacggta caatgcagca gctaagtcac gtgtaatgta tagtgtcgca ccgtctgact
     7201 tcttaatcaa cgctggattc aggtcataag cagataggtc aacgatctct gcacctttat
     7261 cctcgttcag caaatgtttt tcttctaaaa gtttgacgat ttcatccatc ttgtcattgt
     7321 agaaagcttc accgttcaac gaatcgaaac gtacttccag cagatcatag attttattga
     7381 attctttcat cgattcatca cggaaccatt gccataattg aatagcctct ttatcgcctt
     7441 cttccagacg tttgaaccaa gcacgagctt cttcatttaa ttctggttct gtttcagcta
     7501 cttcatggaa ttggacgtat aaacgaagca attcattgat tggttctgct ttaacagctt
     7561 cttctgtacc ccattttttg taagcgacga tcagttttcc aaactgtgtt ccccagtcac
     7621 ctaaatggtt gatgcggatt ggctgataac cgattttttc cataatgaaa ccgatcgaat
     7681 taccgataac tgtcgaacgc aaatgtccca ttgagattgg tttggcaata ttaggagaag
     7741 acatgtcgat tggtactgtt ccttgattcc cgatgttgct atccccataa tgttcttttt
     7801 ctttcactac tgtttgcaaa acttttttgc tgataagttc tttattcatg aaaaagttca
     7861 agtaaggtcc gacaacttcg attttttcaa agttggcaga atcgattttt tctgctaaat
     7921 ctgcagcgat ttgctgtggc gcttttcggt aaacttttgc taatgaaaag gctggaaatg
     7981 caacatctcc gtgttcagct gattttggat tttctagtaa ttgctcgact tgttccagtg
     8041 ttaaatcatc tttgacagcg tcatagacag cttttgcgac aagatcttta ttattcatct
     8101 ttattcctcc taaagtgttc ccttgattgt taaacaaaaa aaatctctag cgacggcata
     8161 cttagtatgc catcactaga gacgagattt cccgcggtac cactccaatt gtcctaaaaa
     8221 aggaccagct ccatctgata tcggtagacg accgtctgtt taaaacagaa ttctccaagt
     8281 gcgcttcgtt gtatcttctt cattcggctt ccaccacttc cgaattcgct agccaagaat
     8341 gatacaagta ctctcttgtt caacaaatct tagtcttatt aaactttctt gaattatatc
     8401 agaaaaaaaa acgaaaggct agtcgtttca taaaaaaaca atccagatgg cctaaaggag
     8461 aagaaatacc acctggatag ctataaatca tttcgtaaat acgaaagact taacaaatta
     8521 atcagctacg ataatcgttc cttcttcaga agctaaaaga ttctcgatat tttctaaaga
     8581 agtgatgatt gccttagcgt ttggttttgc ttcaacaaat gcaatagctg cttctacttt
     8641 cggaagcatg cttcctggag cgaattgctt ttcatcaatg tattgtttca tttctgaaac
     8701 agtaacagtt tctaatttct tttgatctgg tttttggtaa ttgacataca cattgtctac
     8761 tcctgtcaat acgatcagca aatccgcatc gatgatttcg gctaattttt ctgaagcaaa
     8821 atctttatcg attacggctt ctcgtccttc taatccagta gctgtttcta caacaggaat
     8881 cccaccgcca ccaacagaga ctgtgataac tccttgatcg accagcgttt cgatgacacg
     8941 tgcttcttta atcgagatgg gtttagggga agcgactact tttctccatc cacgtccagc
     9001 atcttctttg aatgtcacag tggagtcagc attcatttgt tctttggctt cttcttccgt
     9061 gtagaatgga ccgactggtt tacttggatt tttgaatgaa ggatcatttt catccacaat
     9121 aacttgagtg actaaagaaa ccacatcttt atcgatccct ttttccttca gtacttcgcc
     9181 catggcgttt tgtaaccagt aaccgataga tccttctgtc attgcaacac atgtatctaa
     9241 aggcattgcc ggtgttttct cagaatcagc agcttgctgc tgaatcaata ggttaccgac
     9301 ttgcggacca tttccgtggg agatgatcaa ttcatctcct tgttcgataa atttcactaa
     9361 atactttgct gtttccatta acgcttcttg ttgggctttt gcacttgcat ccgtagacaa
     9421 gatggcattg ccccctaaag cgacaactac ttttcgtttg gccatttcat tcgtcctttc
     9481 ttcagttcta ccgaattttc tagataacaa aacgagccag cgttttgtga ttttccctca
     9541 atgctgactc gttttttcgt gagatgaatg tggttatgct taaacgcgag ggataaacaa
     9601 gttacctaat gttgctgcca tgatcgcttt gattgagtgc atacgatttt ctgcttggtc
     9661 aaattgacga gcatatttgc tgcggaagac ttcatctgtt acttccattt cagtgatgcc
     9721 aaagcgttct ttcatttgtt caccgtaaac agtgtttgta tcatggaaag ctggcaagca
     9781 gtgcaagaag atcaaacgat ctgtgttgtg tgttttttct accatttcca tattgatttg
     9841 gtaaggtttc aacaatttga tacgttcttc gaatttatct tcttcaccca ttgataccca
     9901 tacatcagaa taaaggacgt ctgctccgtc tacaccttta tcaacatcgt cggtaatcat
     9961 caattgtgca ccggattttt cagcaaagcc ttctgccatt ttgacgattt cttcttcagg
    10021 ttgtaattct tttggtgcaa cgatacgcat attcgctcct aaaattgcac ctgtgactaa
    10081 taaagagtta gccatattgt ttcgaccatc tccgcagtaa gcaacagtga taccttctac
    10141 tgttccaaag ttttcttgga tagttaaaaa gtcagcgatc atttgtgttg gatgccattc
    10201 atcagtcaag ccattccaaa ctggaacacc agagaattct gctagttcct ctaccatttt
    10261 ttgactgaat ccacggaatt cgattccgtc aaacatacgt cctagtactt tagctgtatc
    10321 ttctgtggat tcttttttac ctaattggat atcatttgca cctagatatt caggatgtgc
    10381 acctagatcg atcgctgcag tagtaaatgc agaacgagta cgtgtagatg ttttttcaaa
    10441 taaaagcgca atattttttc cttctagata gtgatgtggg ataccacgtt ttttcaagtc
    10501 tttcaagtgt tctgaaaaat cgatcaaata ttgtaattct tcttttgtaa agtctttttc
    10561 tgctaataag cttcttcctt ggaatactga ttctttcata ttaaacattc tcctttagat
    10621 tgtttttatt taagattaaa gaggaaatcc cgacggcagc tgacagtaag actgccgtca
    10681 ggaaacaaat tacttcaagt cttcacggac aagtggttgg ctcatgcagc gtggaccacc
    10741 acgacctcgt gacaattcac ttgaattgat ttctaacact ttgatgccat agctacgaag
    10801 caattcgttc gatacataat ttcggttata agtgactaca acacctggag cgattgccaa
    10861 tgtgtttgat ccatcattcc attgttctcg aggtgcaacg atctcatctc cattaccagt
    10921 tgggatcagt acaagatcat ctaatccaag agcagctttc atcgtttcgt gtaagttatc
    10981 tgaatgagta atcttgatat catcaccatc tggttcgatt gtaaagacat ccatcttacc
    11041 gtctttactt tgaattgctg gatggatagt gaatttatcg tgatctacca ttgtaaatac
    11101 ggtatccaaa tgcatcattg cacggttgtt cggaattttg attgccaaca ctttttcaaa
    11161 tcctgaattt tttgcgaata ggtggcgtgc taatttttct aaagctttcg cattagttcg
    11221 ttgagaaatc ccaacagcta ctactttatc gcttaagatc aattcgtctc cgccttcgat
    11281 atgatctgga ttttcacgat ccaaccatac ttcgattcct ttatttgcaa atcttggatg
    11341 atgtttcaaa atatattctg taaacatcga ttcacgacga cgtgcttcaa aagtcatctt
    11401 attgactgtc atcccatttc ccattgatgc ggaagggtcg cgtgtaaagt acaagtttgg
    11461 cattgggtcc atgtaaaatg gatagtcatc gtctgctgac agatcatata gactgctaga
    11521 acgtacttcg atatcttttg tacgaacccc agccatgatt ttatctacca tttcttgcgt
    11581 ttccattgat agaaggaact catgaagacc ctctcttaca gcattcgatg caatatgaga
    11641 ttcatccaac attttattca agaattgttc tttgacattg cctgcgtcaa tcgcttctgc
    11701 cgctaatttt tccaagtaaa gagtctcgac gccttcattt tgcaatgtct tcgcaaagtt
    11761 gtcatgttct tcttgagcaa tcggaagata tggaatatca tcgaacaaca gacgatccat
    11821 aatatctggt gtcaaatttt ccacttcttg tccaggccgt ttcagtaaaa ctgtctttaa
    11881 tttccctatt tcagagaaaa cgtgaatagg tttatccatg ttgtgttgcc tccttctttt
    11941 tgattacatg tataggatat cctccttttg taatcacttt cataattttg tctcattcaa
    12001 aatgagacat tcctcacatt aatattcgat aattaaataa ttaaacgttt ttctattttt
    12061 aagcg
//
LOCUS       DAYYCY010000078        11988 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000078 DAYYCY010000000
VERSION     DAYYCY010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11988
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.078"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            82..2100
                     /locus_tag="ACHAZB_001696"
     CDS             82..2100
                     /locus_tag="ACHAZB_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFM8614343.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    2170..2376
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2527..4941
                     /gene="leuS"
                     /locus_tag="ACHAZB_001697"
     CDS             2527..4941
                     /gene="leuS"
                     /locus_tag="ACHAZB_001697"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFM8614344.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            5289..6842
                     /locus_tag="ACHAZB_001698"
     CDS             5289..6842
                     /locus_tag="ACHAZB_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HFM8614345.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(7223..9007)
                     /locus_tag="ACHAZB_001699"
     CDS             complement(7223..9007)
                     /locus_tag="ACHAZB_001699"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8614346.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(9022..10743)
                     /locus_tag="ACHAZB_001700"
     CDS             complement(9022..10743)
                     /locus_tag="ACHAZB_001700"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8614347.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            10843..11604
                     /locus_tag="ACHAZB_001701"
     CDS             10843..11604
                     /locus_tag="ACHAZB_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM8614348.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         3932 a         2163 c         2427 g         3466 t
ORIGIN      
        1 aagctcttaa ataactttta ataaatatta agagaagtta taattattaa taatagataa
       61 attgaagttg gaggatagaa gatggaaaac attgcaagaa aagaacggag acgtttagaa
      121 cagaccaaac gttttcgaaa agcaaaacga ggggcagcaa tcgtgggaac tgcgatggta
      181 ggctgttctg tcgctgcccc attagtacag ccggttcctg ttcaagcgga tgaaacacct
      241 gttcagttcg gcgcaagaat caatacagcg gcatttatca cagaaattgc tacttatgct
      301 caaccaatcg ctcaagccaa cgatctatat gcatcagtga tgatcgcaca agcagttgta
      361 gaaagcggtt ggggcagtag tgcgttatct caagcacctt attataatct ttttgggata
      421 aaaggcagct atcaaggaca aaccgtctac atggatacgc ttgaattttt aaataatcaa
      481 tgggtaaata aaaaggagcc tttccgacaa tatccttctt ttgctgaatc tttcaatgac
      541 aatgcctatg tacttagaaa tacttcattt ggcaacggtt attactatgc cggtacttgg
      601 aagagcaaca cgaaatccta tacagatgcg acagcttgtt tgacagggcg ttatgcgact
      661 gatcctggtt acgcaggcaa attaaataat atcatcgcaa cttacggatt gacccaatat
      721 gatacaccag ctagcggaaa cgcaggcggt ggagcgacta caggtggcaa taatactgga
      781 agtgcaggta gtactggcgg ttcaagcaat tctggtaata caaactcagg ttcagcaact
      841 tcgaccactt atactgtcca atctggtgat tctgtttggg gaatctcaaa taaattcggt
      901 atcacaatgg atcaattgat ccaatggaac aacatccaaa acaatttcat ttatccagga
      961 caaaaactga caatcaaagg tagccaggaa aatggctctt cgacgaataa ttccggtaac
     1021 aataccaatt cttcaggaaa tgctggtaca tcaaacggag gacaaacaac tggtgcgaaa
     1081 tacactgttc aatctggtga ttctgtttgg aagatctcaa atgatcacgg aattacgatg
     1141 gctcaattga ttgaatggaa caatatcaag aacaatttcg tttatccagg acaacagcta
     1201 atcgtatcaa atggcggttc ttctaatgct ggagcaaata ctggctcaaa tactgggaat
     1261 acaagtggct ctagttcgtc cagctcaagt tcttctaata cagcttcagg aacgaaatat
     1321 acagttaaag ccggagactc tgtttggagc gtctcaaata aatacggaat tacgatggct
     1381 cagctaatcc aatggaacaa tatccaaaat aactttatct acccaggaca acaactgact
     1441 gtttcaaaca gtggatcaac aaacagcacg tcaggtaata caggttcgtc aaccaataca
     1501 aataacagca gttcagttaa tactggcagc agttcgtctc aaggcactta taccgtgaaa
     1561 gcaggtgaat ctgtttggag cgtggcaaat aaaaacggca tcacgatgga tcagttaatc
     1621 gaatggaata atatcaaaaa caacttcatt tatccaggac agcaattgat cgttaaaggt
     1681 ggaacttcgg caaatacaaa tacaggttca actacttctg cagcgaaacc aaatacaccg
     1741 aatacgtctg ctacgacaag tacatcatcc actggtaata cgatgtatac cgtgaaagcg
     1801 ggcgagtcag tttggagcgt agcgaacaaa catcatatca cgatggatca gttaatcgaa
     1861 tggaataata tcaaaaataa tttcatctat ccaggacagc aagtgattgt gaagaaaggt
     1921 tcatctcaat caacaaacca acaatcagtt actggttcga aaacctatac tgtgaaatct
     1981 ggagaatctg tttggagtgt tgctgacagt catggaatta caatggctca actaatcgaa
     2041 tggaacaata ttaagaacaa ctttatttat ccaggtcaaa cgttgattgt agctaaataa
     2101 aatagctgtt cgaagaaata acgattgaat tccacaaaaa tatttgttac tataaggcta
     2161 cttaaatatg acaacacttg aaaaggaagt agtaaaaaga ctgaagtttc agagagcgca
     2221 tggctggtga gaatgtgcac ggatgctttt gaactcgcct ttgagttgcc cttgctgaat
     2281 acccgagtaa gcaagcggcg gtgacggccg ttacaacgtt cgaggccttt tcactaaggt
     2341 aaaattaggt ggtaccgcgt agattacgtc ctataaaaag agcaatcttt ttataggatt
     2401 tttttatttc ttttgtgaag tttgagaaaa caaagcaaaa ggaaataaaa ataaacagat
     2461 tctgccggtt ttttcgttat atttataata gaaacaagtg ttttcaatag aacaggagga
     2521 cagcaaatga gttacaatca taaagaaatt gagaaaaaat ggcaaaaata ctgggcaaaa
     2581 acgaatactt tcaacaccca tgatgatcct gaaaagccaa aattttacgc actggatatg
     2641 tttccctatc catccggtca agggctacat gtagggcatc ctgaaggtta tacggcaacg
     2701 gatatccttt cacgtgtgaa aagaagtcaa ggatacaacg tgctgcatcc aatggggtgg
     2761 gatgcattcg gtctgcctgc tgaacaatat gcgctagata caggaaacga tccagctgag
     2821 tttacccaaa aaaatatcga aacattccga cgccaaatca attctttagg atttagttat
     2881 gactggaatc gcgaagtgaa tacaactgat ccggagtatt ataaatggac acaatggatc
     2941 tttacgaagc tttatgaaaa aggtctagct tatgaagcag aagtagcggt aaactgggta
     3001 cctgaattag gaacagttat ttcaaatgaa gaagtgatcg atgggaaaag tgaacgtggt
     3061 ggctatgatg tagtcagaaa accaatgcgc caatggatgc tgaaaatcac tgcttatgct
     3121 gatcgcttat tggatgatct agaattagtt gattggccag aaagcattaa agaaatgcaa
     3181 agaaattgga tcggtcgttc agtaggagca aatattgaat ttaaagtggc tggtacggat
     3241 aaatcctata ccgtatttac gacacgacca gatactctat tcggtgcaac ttactcggtt
     3301 cttgcacctg aactagatct tgttagagaa attacgaccc ctgaacaaaa agctgctgta
     3361 gaagcttata tcgaagagac agctaaaaaa tcagatctaa agagaacgga tcttgcaaaa
     3421 gaaaaaacag gagtatttac tggtgcttat gcaatcaatc cagttaatgg caaggaaatc
     3481 ccaatctgga tcgccgatta tgtattatct tcatacggca caggagccat catggctgtt
     3541 ccagcacatg atgaacgtga ctttgaattc gctcaaactt tcggactaga aattcttcca
     3601 gttatcgaag gtggagatgt tgaaaaagct gcttacacag aagatggtac gcacatcaac
     3661 tctgaatttc tagacggcat gaacaaacaa gaagcaatcg ataaaatgaa tgaatggcta
     3721 gaagaaaatg gcgtagggaa aaaagaagtc agctatcgct tacgtgactg gctattctcc
     3781 cgtcaacgtt actggggtga accaatccca atcatccatt gggaggacgg aacagtcaca
     3841 gctgtcccag aagaagagct accattacgg ttgcctaaga caaaaaacat caagccaagc
     3901 ggtactggag aatctccatt agcaaatatc gaggagtggg tcaatgtcgt tgatcctgta
     3961 acaggtaaaa aaggccgtcg cgaaacgaat acaatgccac aatgggcagg aagctcatgg
     4021 tattacctgc gttatatcga ccctcataac aaaaaagaac tagcagatta tgaaaagttg
     4081 aagcgttggc taccagttga catctacatt ggtggagcag aacatgcggt acttcattta
     4141 ctatatgcac gattctggca taaattctta tacgatatcg gtgttgttcc tacaaaagaa
     4201 ccattccaaa aactatacaa tcaaggaatg atcttaggcg aaaacaacga aaaaatgtca
     4261 aaatctcgtg gtaatgtagt caatcctgat gatgtggtcg aaacttacgg tgcagataca
     4321 ctgcgcatgt acgaaatgtt catggggcca ttagatgctt cgattgcttg gagcgaaaat
     4381 ggcttagaag gaagccgtaa attcttggat cgtgtatggc gtttgatcgt tgatgagaac
     4441 aataaaatgc gtgaccgtat cactacattg aatgatggca agcttgataa agtgtatcat
     4501 caaacagtga aaaaagtgac agaagactat gaaaatcttc atttcaatac agcaatctcc
     4561 caattgatgg tctttatcaa tgaagcctat aaagtggacg ctctgcctta tgaatatatc
     4621 gaaggatttg ttcaactact cgcaccgatt gctcctcaca tgggtgaaga actatgggca
     4681 atccttggaa atgatggtgg tatttcttac gcaccatggc caacatacga tgaagcagca
     4741 cttgttgaag acgaagtaga agttgttttc caagtaaacg gtaaagttcg tgcgaaaagc
     4801 aatgttcccc gagacttagg aaaagatgag ctagaaaaag cagcattagc caatgaaata
     4861 gtgcaagaat atattgaagg caaaacagtc agaaaagtta ttgcggtacc aaacaagtta
     4921 gtcaatatcg ttgctaatta attggctctt tgtcaataag gactgatgag ttgtgcaaaa
     4981 tcaaatctgg gccagaatga acttcattct gtctcagatt ttttgttatt tcaaattgat
     5041 taattgattg atcaaaaagg ttttgatttt catgttctca aacacgttca atgtcttttt
     5101 cttctgaatg gtcttgtaag agatctctgt aatcatcaaa tttttgtcta ttaatcttag
     5161 tattttttga tgtataaact acctgaaatt gagagtacat accaaattca taaatttttt
     5221 attagtggga ctagtgattg tatggatttt gacgcataat gaatgaagta tgagaaaagg
     5281 agagacaaat ggatcaagaa ctatttatta tcgaagtatt atcagctaaa caaaaaatta
     5341 tcgagaaagc gaaaaaaaga gcgaaaagaa taatgttcaa gcttttaagc ttagtatttt
     5401 tctctggttt gctttttttc ggagctgtat cgttcctata tcgccatatg agtgcagtag
     5461 tccgttcagc tgaagggatg caaaaattaa ataatttgta tcacttttcg tggggagttg
     5521 gcggaataac aattgttgtg cttattgttt ttcttctact tatttggctc cgttaccatc
     5581 aattgtcgcg tcatttcgat caatggctta gtcaaagagc acaacagttt ttacataata
     5641 aaccaacttc tcaagatgat gactatcttt attttgagaa gacttcgacg agtcaagctt
     5701 ttcggataca aaaaagtgat tgccagttat tgctgagatc acttgatgat ttacctatct
     5761 acttaggcga aagcaaatca aaattcggaa tgaacacatt ccatattttt ttactagaaa
     5821 acgaacccag atgggaaaaa agccagtctt cgcctggtgg atacctgaca actaagatga
     5881 gtacattagt cactggtttt ttcctcattc ttttagctgg aacaatctat tatgggagat
     5941 acactcagcc aaaaatgtct tctcatataa atgctgcttc tcacatgaca atgaactcaa
     6001 ctaacgatac gcatatacaa aagacaaatg aagggttgtt agctgatcct aagcaaaagg
     6061 taaatttgac aacacaaaag ataaatgatc tcaaacttga tacagagacg cactctctct
     6121 atatgacaac agatagtggt gctaattggg tatttgtgcc gattgattcg caatggttgc
     6181 gttttggcga atacaccctg acaacggggc tgattcctga aggttattgg atggacaaca
     6241 catatgatat ttccgccgat ttctcatggt ttatctattc ttctggggat gatgtacgtc
     6301 tgctgacttc aagagataat ggtaaaacat ggcaaacgtc tattatcacc caatcagttc
     6361 agccgatacg ttatcgaaaa gcggtatttt atggcaacga tggtctattt atctgtacat
     6421 cagccagtgg gatgtctgca gaaactttgt acatttacac tactatggat ggcggaatca
     6481 cttggataaa aagtggaagt acaatgatca atcagccagt acaaaatgtc agcttcgtta
     6541 caacttcttt aggttttgtt tcgacacgtg aaagtttgtt ttatacaaac aacggaggac
     6601 gctcattcaa agaatcagtt ctgtcgattc cggaaaacta tcagacaggg gggatagatc
     6661 tttttcaatc accaaatgaa gttactcaaa taagcgcgaa tacgttagag acaaaatttt
     6721 atttgttgaa aaaaggacca atcgatgcag ggaaaatgtt tgcctgcctc ttccgttcaa
     6781 ctgataacgg ggaaacatgg caattcgttc agcaactatc gcaagtagaa caaggagatt
     6841 aaagaagaga gattgtgagc ctgacgttca gcaccgagtc acaagaaaca aattcaaaaa
     6901 acagcttctc atatttgttg aatagtgtaa ctcgtgattc ggagttgaca agagcaacaa
     6961 gaagcgattt tgaagtatag cttctcgtat tttacaagat cgtgtcttat tgccgatagg
     7021 ttgtctcttg aacaccgttt attcgtaaaa agggattgtg acaagtcttt tgtcactact
     7081 taccgaacga aactgaagga gctatccaaa aagtatcatt agtattattc tttgtgtttc
     7141 tagtggcacc actagtacat ttaaaaaagc tatcgatcat ggactaatca tacatgatcg
     7201 atagcttttt agataatgtt tgttacgcta tctgaaattg actattataa agactggcat
     7261 aaagcgttgg tgattggagt agttcagaat gtgttccttt ttcgacgata tcgccattct
     7321 tcattacaag gatcatgtct gcattttcaa tcgttgacaa acggtgtgca atcacaaaac
     7381 ttgtcctatc ttttgtcaca gcttccattg cttgctgaat caatgcttct gttcgtgtat
     7441 ctacgctaga agttgcttca tctaaaataa cgacaggagg attcgctaga atgactcgag
     7501 caattgtcaa taattgttgc tgtccttgag ataacgtgcc gttttcgcta gaaatgatcg
     7561 tatcatatcc gtctggcagt gttcgaataa agtgatcaca ttgtgcgatt tcagccgctt
     7621 cgataatttg ttctcttgtg gcatccattt tcccgtaggc gatattatca gctaccgttc
     7681 cttcaaacag ccaggtattt tgtaaaacca tcccaaaaga acgacgtagt tctccacgtg
     7741 aaagcttggt aatatcaaga ccgtcgaatt cgatctttcc tttattcagt tcataaaaac
     7801 gcatcagcag attgactaaa gtcgtttttc ctgcaccagt tggaccaacg atcgctaccg
     7861 tttgttttgg ctttactgaa aaatcgactt gattcatcaa tattttatct gatgtgtaac
     7921 caaactgtac atttttgaaa gctatttttc ctttcggttg ttctaaaagt cttgcatctg
     7981 gaaggtctgg aatttcatct gtttcatcta atatctcaaa tatcctatcg attgctgcca
     8041 ttgcggattg gatcgagttg atcacataag aagcggtaga aattggttca gaaatctgat
     8101 tgatgtactg aagataagct tgtaagaagc cgatcgtcac accacctgat agaacaagca
     8161 tagcaccgaa taccgcactt atgatgaagg cgagttgatt aatgaaacga atggccggat
     8221 agatagcaca gttcataaat tgagcacgtt taaaagcgtt gtattgcttt ttatttactt
     8281 cgtgtatagc agcttctgca tgagcttgct gattgaatgt tttgaccact agattaccag
     8341 ctaagatttc ttccattcga ttatttaaaa cacccagttc atactgattc tgttcagaaa
     8401 tctgtttgtt tttgttcgca atcttagttg taacaattga actgccaacg atcagcaata
     8461 agacgagcaa agtaagcttg ctttcgatat aaaacagcat cagaccagca gcagctattg
     8521 ttacgacaga agtaaaaaat tgattgatac ctgtcaataa cacttgagaa agttgattga
     8581 gaccggtagt tgtccgacta atgatgtcac ctacttgatg attgtcaaaa aacgacatcg
     8641 gtaatgtttt gaattttttt gtgatttctt ttcgcaccct caatgtgatc ttttcgctta
     8701 acgctgccat tgtccgttcc tgaataaatg aagtgatgct gcttaagact gaacagccga
     8761 tcagcaataa gacagggaac aacaatgctt gtttgatatc actgaaagaa agctgtccaa
     8821 tacctgtttt tcttatttct gataagagat catcgatccc gattcccatg atcaagggca
     8881 tggctacgac taataggttc ccgaataaac tgcatagaaa caatccgtaa aaatatttat
     8941 gttcaggttt gatcattttc caaaaacgta tcaaagcacg cgtgttcaac gtttttttct
     9001 tttccatcgt atcccttctt tctatattcc ttgtgatttt gcaaactcct gataagtttg
     9061 gcaattagac agtagttctt catgagtacc ttgtccaacg atttttcctt catctaaaac
     9121 aatgatgtta tctgcttgtt ggattgtact gaggcgttga gcaacaatca cgagagtttt
     9181 ctccgacatg tgtttatgaa gggctgctct taatgctgca tctgttttat agtctaaagc
     9241 ggagaaacta tcatcgaata catagatctc tgctggtttg atcaacgctc gagcaataca
     9301 tatccgctgt ttctggcccc ctgaataatt agaaccgcct tgagcaacaa agctgtccaa
     9361 tccgtcagga agggtgtcca ctagttcttc taattgagcg atcttgatcg ctttagacaa
     9421 ctcttctgtt gaggcatatt catttcccat ctgcaagttg cttaaaattg tcccgctgaa
     9481 taagaaagcc ttctgaggga cataactgat gtgggagcgg atctgttctt gagtcatttg
     9541 agtaagagga ataccaccaa gggttacttt tcctatcgta gggtcactaa gtctcaagag
     9601 aagtttcgcc aaactacttt ttccagatcc ggtcccgcca acgatcgctg ttgtttttcc
     9661 tttaggtatt gtgaaatcaa tatcttctag aactggatca gagatttcag tatatttaaa
     9721 tgtgacatgt tcaaaagcag ctgcatttgt ctgatcaaag gttggttcag ctactgtttt
     9781 gatcgtctct tcctcaattt cgatctctgc ttgcagtatt tcttctatcc gtcgtaatga
     9841 ggcaaacgat ttagggataa tgaccaatac catagctgcg atcatcaagt aagcaagtgt
     9901 cagcatagag tattcgacta cagcagtcac gacaccgatt tgtaatgctc cttctgctac
     9961 aagatttccg ccaaaccaca aaatagaagc ataaacgatt cccatgagca aaaaagcaat
    10021 tggagtgacc caagcaaata ttcggttcac tttgatcatt tgattagcat agtcagcaaa
    10081 gctcttattc gttcgctttt cttcatatga ttgattatca aaggcacgga tcatattaat
    10141 cccagtaaag aattctctca atgtgatggt gatccgatcc atttttggct ggatactcaa
    10201 agataaaggt gcccctcgtt tcatcagtcg ataaacaatc catacataaa aaacaacaga
    10261 gatcaatgta acccatgtta gtaatagaga ataacgaaac atcatgagaa tggtgaaaac
    10321 gcaaataacc ggtgctggta agatcatttg gcaaaataaa acaatcattt gctggacatt
    10381 atcgacatca ttcgtcattc ttgtcaaaag agatccagaa ctaaaacgac tgatatcctt
    10441 cattgacagc tgttggaatt tacggaaata tttctcccgc gtttggaatc caaacctagc
    10501 ggaagccaat gctgaaaagt aactagaggc aactgcagcg attgttccta gaaacgctac
    10561 aagcagcatt tcaactccaa ttttttttat aactgaggct tcgccacttg ctatacctgt
    10621 gtcgatcagc tttgctacta attttggtac acctaatgta ccggcgattt ctaaacagat
    10681 catcacaaag gtaaggagag cgagtccttt gtttttctgt aagaatgttt ttaagataga
    10741 catattattc ctcctttttt cctgatactt ttcgattcta taccttcaag taacttgaag
    10801 gtcaatagaa aaaagaataa actgaaaaag aggtgagaaa aaatgaaaaa aatgtatacg
    10861 attggccaag tatcggaact atttgatttg ccaaaatcaa cgatccgcta ttgggatgaa
    10921 caaggattaa tccgttcaag tagacaagaa gagaatgatt atcgattgtt tgatatcgat
    10981 gatattttta tgctttatga tatcgttttt tatagaaaat tagatatacc gatcaaacag
    11041 atgaagaatt tatatggaaa aacattgacg gagttatatg agacacttga tgaaacagaa
    11101 agaagaatcc ataaagaact ggtggtgatg aaacaaaaac aaaaagagat aagggagcaa
    11161 aaaaaacagt tgaaattgat gatcgataca aacgaggaag aatttcctgt agaagagatc
    11221 ccttttgact gtatgatctc aacggagttt gaagatattg tggaaatcaa aaaatttcta
    11281 cctaactatt caagctttgg gatgatgagt atgccagctt ccagttcaca gacgatgtat
    11341 ggatttttta tcgaccctag tgaagtacat ttatttactc aagacgtgat ttgggagaaa
    11401 aaagatacgg ctgtttatcg tcgattctta ttgaaatccg agatgaacca tgcggaacgt
    11461 aataatattt tagaaatcag agaaagaatg tatgaaaaag gatggaaaac aggagaagtg
    11521 atcggccagt atctattgac gaatacggat gaaaacaata tcagaactga atattatcat
    11581 gcatggatag aaatgaaaaa ataggaaata tgtgtttaag ataactcgaa aatgatggtt
    11641 gttttagtta taaagaaaaa ttttctggtt cgattacggg tttaatgtgg gggattatta
    11701 gtcgtggata gatagaattt tagtttttta gaggaaatat gtctttaaaa atggaatggt
    11761 tgcaattctt agcgatatat acgatgtaat tgtcctctta aaaaagtatg gaaaaataat
    11821 tgtattgggt gttatgtata attcatggtg aactgagact aatgagaaga aaaattcata
    11881 ttgttatgag tgagattgta tcagatgaga atgataaatt aaataggtgc ataaaagcat
    11941 tacacttaaa atacattgtt gcttacatta ttacaatatg ttaataag
//
LOCUS       DAYYCY010000079        11667 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000079 DAYYCY010000000
VERSION     DAYYCY010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11667
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.079"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(37..216)
                     /locus_tag="ACHAZB_001702"
     CDS             complement(37..216)
                     /locus_tag="ACHAZB_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8614349.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            complement(232..432)
                     /locus_tag="ACHAZB_001703"
     CDS             complement(232..432)
                     /locus_tag="ACHAZB_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8614350.1"
                     /translation="MNKIIPLLFVLFVGEILMRHGAALHLNFVVFLGILVFCLIFWRF
                     VLTLVSLIFKLIAAIFLIGLFR"
     gene            complement(597..1304)
                     /locus_tag="ACHAZB_001704"
     CDS             complement(597..1304)
                     /locus_tag="ACHAZB_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8614351.1"
                     /translation="MNKVVYQLTTEDVSQFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNRLDVYKRRPDTLKKGAELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELTVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGQ"
     gene            complement(1297..1734)
                     /locus_tag="ACHAZB_001705"
     CDS             complement(1297..1734)
                     /locus_tag="ACHAZB_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8614352.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFENIEKNGAFYTIESEEKDNE"
     gene            complement(1749..2000)
                     /locus_tag="ACHAZB_001706"
     CDS             complement(1749..2000)
                     /locus_tag="ACHAZB_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8614353.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            complement(2000..2206)
                     /locus_tag="ACHAZB_001707"
     CDS             complement(2000..2206)
                     /locus_tag="ACHAZB_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HFM8614354.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYVLIDEKVKKR"
     gene            complement(2643..2903)
                     /locus_tag="ACHAZB_001708"
     CDS             complement(2643..2903)
                     /locus_tag="ACHAZB_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8614355.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIESMQIRNPDHFTRSMGI"
     gene            3077..3181
                     /locus_tag="ACHAZB_001709"
     CDS             3077..3181
                     /locus_tag="ACHAZB_001709"
                     /inference="COORDINATES: protein motif:HMM:NF028245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HFM8614356.1"
                     /translation="MPVETALGLMISFGSLIATIIFGILAVVNNNQKK"
     gene            complement(3322..3807)
                     /gene="ssb"
                     /locus_tag="ACHAZB_001710"
     CDS             complement(3322..3807)
                     /gene="ssb"
                     /locus_tag="ACHAZB_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFM8614357.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSSSPSPTTFEQRDVATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            complement(3810..4709)
                     /locus_tag="ACHAZB_001711"
     CDS             complement(3810..4709)
                     /locus_tag="ACHAZB_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFM8614358.1"
                     /translation="MTPTQFAAKTRNLAKSTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTEQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIRGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            complement(4722..5315)
                     /locus_tag="ACHAZB_001712"
     CDS             complement(4722..5315)
                     /locus_tag="ACHAZB_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HFM8614359.1"
                     /translation="MQDKLKELLAIRDGNLSMKEARKFGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEFEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            complement(5391..5540)
                     /locus_tag="ACHAZB_001713"
     CDS             complement(5391..5540)
                     /locus_tag="ACHAZB_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8614360.1"
                     /translation="MRNTTHKQQEIENQKAKIRLEQEKLRANKRRLQQLKNQQRIQKE
                     IELDR"
     gene            complement(5556..7760)
                     /locus_tag="ACHAZB_001714"
     CDS             complement(5556..7760)
                     /locus_tag="ACHAZB_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HFM8614361.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADHGKIFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGTIHQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKTVGKVPVDLGWKKLFGK
                     ESDRKEKEEEPLLPKVTKGEAVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEATRHLGVCPKCREQEVLLYQNVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNGRTSQSVRGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNYKKRTRRTTK"
     gene            complement(7771..9231)
                     /locus_tag="ACHAZB_001715"
     CDS             complement(7771..9231)
                     /locus_tag="ACHAZB_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFM8614362.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVRDDRLDHAVAFLWKDPQTNETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKVI
                     YEEVAKEEKITPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQLSVNDSIEAINLKD
                     ICREVAKELKACERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIYQQEIRKQKRAMLFQQGRQQERE"
     gene            complement(9292..11664)
                     /locus_tag="ACHAZB_001716"
     CDS             complement(9292..11664)
                     /locus_tag="ACHAZB_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFM8614363.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERLENKVHSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKDYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKENDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQTVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAEQKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNSFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
BASE COUNT         2892 a         2432 c         1957 g         4386 t
ORIGIN      
        1 tgctatcact tttagttttt aggataaaaa gtgacgtcac tttttgagtg ctggtacctc
       61 aaatcctgct ttttcttggg caatcaataa atctaacgca atacacataa tcctggattc
      121 acttttgccg tacttctgct ttagttgctg caattctttt tgtctctttt catccaacgt
      181 cacgggtaac cgtttatttc ctattgcaat tggcattagt actctctcct ttcatcgaaa
      241 taaccctatt aaaaatatgg ctgcaatcaa cttgaatatt agactcacaa gggttaacac
      301 aaagcgccaa aagatcaaac aaaataccag tattcctaag aatactacga agtttaaatg
      361 tagagctgca ccatgacgca taagtatctc gccaacaaac agtacgaaca atagaggaat
      421 gattttattc atatggatct ccttctttct cttaggttat cgggctttgc ccgcttattt
      481 cgcaccacct aagcggcggg ggcggttgga tcgattggtg cgaaataaga accattccct
      541 taatttcaag gttttttgtt cttatttcac acatcctcac ttttcggtat aaatggttat
      601 tgaccttttt tcctttcaaa tcgttgatat ggcattcgtt tacggtcaag ttcattttgg
      661 atttcttctg tatcgtcgta agtgtaggtg tctaaaaaat cagttaaagt cgtgtaccag
      721 tcgtcgcctg ggttatctac ttcttctaca tattccctca aaaatgcttc gggtacagtg
      781 agttcataat ccattcccat taacacgata gattccgtcc cacacgtcaa aaatctagta
      841 aatacaaggt aataatcgtc taccgtcgca tctttgctgt ttagctcttc catttcttct
      901 tccattaatt ccatgctctc aaaaattgaa taatattctg attctgagta atagtgtttc
      961 ctgcaaatcg tgtagctgac aaagttttcg accagctcac tcctttctag gtaaacattc
     1021 atgatatatt tttctgtttc tagtcgtaga agctccgctc ctttttttaa tgtgtcagga
     1081 cgacgtttat acacgtctaa acgattatgg ctgactgtaa ccttcgtttg actcgtttca
     1141 ctttccatat acaactgttc ttccactctt tgaataaagt acttcatttc ttccatacaa
     1201 ctaaagtctt tttgaatccg tctgattact tcattcaagt gttctttgtt tgttaaaaat
     1261 tgcgaaacat cttctgttgt tagctgataa accaccttat tcattatctt tctcctccga
     1321 ttcaatcgta taaaatgcac catttttttc gatattttca aaagtatcta gccagtcgct
     1381 agccattgct ttagctttct taatctgtgc gaattttgca taagtcagtt ctaaggcttg
     1441 atattcggaa ggtttgatag tgcttggttt gacacctact tccaagcata gtaaggcttg
     1501 gaaaaaccat tttccatttt gttttttcag ttcttccagt gttcgatagt acgtcgcaat
     1561 gagattcatt tcgacacctg attcaatcat ttcttccact gcgactgaca atttttcctt
     1621 gtaatgtttt ttgggtacac gtgtttcatg gtataggaac gtcatatcct ccattttttg
     1681 tgccatttta tccaaaggta atttgacaaa ttcgtcatag taattttgag ccatattttt
     1741 tctccttttt agttctctaa tttggttaac caatattcga tttcataagt ttccatgttt
     1801 tcttgataat gcagttcata tcctgcaatc ttcactaggt cttcaaatag catagtcggt
     1861 gcatcctcta ctaaaggggt ttctatcgtc cagtaccgcg gaataagtaa agcttctgct
     1921 actttttcag aaatgacttc tttgtccacc aaaatttgaa cgacttcttt tacagtcaat
     1981 aatttgtgtt gtgttttcat caccgtttct ttactttctc atcaatcaaa acataatcgc
     2041 cataagacaa ggtatatttt ttcccgcatg ttggacattg atacaaacga tagcttttga
     2101 tacatttcgc tctttctcca caatcgcatt cgacagtatg tggtgcatga tagtttggga
     2161 ataattcgct gaagggaaca ccccaataat caaatttctt tcgcatgatt gattctgcct
     2221 cctcttagga aaggttttat ttcctttcgt tagttctttc tttattgatt tgttggccag
     2281 aatgaatcaa cgaaaagcga caaggacaag ggcgcgcagc gtgccattta cccttgtcct
     2341 tgtcgctttt tggtgataaa aggcaacttg ttgccggtct ggcaaatcaa taaagagata
     2401 gcgaacagga acgaaccttt tggtgagagg aagttttttc tattttgtct tgctttgcaa
     2461 gacaccatat cttagtgaac gtgcaacgtt cacttccttt cttctcgaac ggcggtggcg
     2521 gttggagtga gagaaaggtt tttcaattga gaaggcactt gtgtcttcct aaaaaggtct
     2581 tttcctgttt ctatgtaaaa caaaaagagc tgcaatcctc atttaggact gcagctcttc
     2641 ttttatatgc ccatcgatcg cgtaaaatga tcggggtttc taatttgcat cgattcaatg
     2701 actaattgat tccgtttatt gaaatgacct gtgacggcaa tattgtattt tccatcatcc
     2761 ataattaaca atttatcgat aatttcaata tctgcaacta cacaattaat cgattcatga
     2821 attgtttgta aagtaaagcg aaccaataat ggttgtgttt ttattactcg gattttatca
     2881 agcaaaccaa catatttgtt catcacgatc acctcgctaa aattatagca aaaacagcta
     2941 aaattgagaa tttttattcg attttagata tgctataatg atcaagacag taaagtttgt
     3001 gacggtggct atccgattga aagtgaggtg gtgcgtatgt gcgtacttcc aaaatcttac
     3061 agaaaggaga agccttttgc ctgttgaaac ggcgctagga ttaatgatta gtttcggttc
     3121 tttgatcgca acaatcattt ttggaatctt agcagttgta aacaacaatc aaaaaaaata
     3181 accgtcagct ttagccggtc gacgattatt tttcaaaaat aatattaagc tggtcaccgt
     3241 cttaaacggt gctgttaggg gagttgttag cgcaactcct cttttcgtat ccattataac
     3301 aactttattg atcttttaca atcagaatgg taaatcatca tcactaatat cgattgattg
     3361 tccgccaaat ggattttgtg aagcgttcaa tccattgttg ttaggcgtag ctacatcccg
     3421 ttgttcaaag gtggtagggc ttggactgct ggacgattga tcagcgtgcg cacggctttc
     3481 tgtcgctgct ttactttcta atagttggaa attatcggcc acaacttctg tcacatacac
     3541 acgttgtcct tgttggttat cgtaggttcg ggtttgaatc cgtccaacaa cgccgatcaa
     3601 actgcccttt ttagctaatg tcgccatcgt ttctgctggt ttgcgccaaa tcacacagtt
     3661 aataaaatca gcttctcgat tgccgttttg attcgtgaaa tttcggttga cagctagggt
     3721 aaaagtcgct actgcagtac cactcgcggt ataacgtaaa tctggatctt tggttaagcg
     3781 tcctactaaa gttacattat tgatcatctt tatctctcca tttcttgatt catttgttgt
     3841 gatcttttaa actgccgttt tctttcttga ttttcaattt tgatcacact tttcattagg
     3901 tgatcaattt cgttgactgt atctgtaaat gaaatggaat tcgcaaacgt gttaactgag
     3961 cgatattttt cccactgctt ttccaaatag tcatttcctc tgatcttatc catttctctt
     4021 tcataataaa aggacaacgg ttttgtatcc ccacgttttg taaatgtatt tcgaacactt
     4081 ttgtataact ctttcgtatc gatattttct agtttcggaa tggtaaataa gtcgtagtaa
     4141 tcttttgctc tcgaattatc tgtgccaaag ctgaacgtag cacttagctt ttctgcaata
     4201 atttgttctg ttggataagc tggaatctcc acttttttat cttcaaacat taattggtga
     4261 tagtgtgtat ctacttcagg ataaatagaa tctcctgttg tcacgtccat tttaaacggt
     4321 acacgtactt tctctaaatt agccaccaat ttaatttgaa atccaggata aaaatcggct
     4381 tctcttgttt ccttcattcc ttgaatagag aaaacaatgc cttcttttgt gggttttcta
     4441 gttaaatcat taaaaatgct cttcaatttt tcttcagtta atttgctatt tctaaatgtc
     4501 gtatcgatat caattgttgt ccttctatcg attccatatt ttgatcctaa taaaaagccg
     4561 cctttcaaaa tgaagtcatt tttgtacttt gattcagaaa tttgttcaat tagacgatcc
     4621 atcataaaat gccgttgcac taactgtgca tttaatcctg tactctttgc taaatttctt
     4681 gtttttgctg caaattgtgt cggtgtcatc tttttcctcc atcaatataa gctttgcatg
     4741 tggtatttca tttctttttc tacatgtagt tttgtcatgt aatctcttaa tttacttgga
     4801 tctctcaatg ggtcttccat gtactctttt aatgcttcta atttcatttc atattcaaat
     4861 tcatatctcg gattccaaat atcacaaagt gtacgctctt tatcatacat agtaatagga
     4921 tttccttgaa aggaagttcc ctctactttt cctagttcat aaacttcttt cttcacgaaa
     4981 tgaaattgta actgctgctc ttttgtaatt gaacgatcaa cgttatagcc atggggaaaa
     5041 gttaagtgaa tgaatttcgg catattcgta gaaagctggt aaatatctaa agtcgtttca
     5101 tgagagaaga ttcccctgga aaatcgttgc tgaagccaaa ataaatcatc tattccatga
     5161 ccatcctcta cataataacc acgatcaact ttgatcaatt ttccttgatt caccaatctt
     5221 tgtacagtgg ttgctgcgat tccaaatttc ctggcttcct tcatagataa atttccatcg
     5281 cgaatagcca acaattcttt cagtttatct tgcatttact tcccctccct tcgttacaaa
     5341 tacactacat caaatatatc atagtagttt atttgtaaca agattaacgt ctatcgatct
     5401 aattcgattt ctttttgaat gcgctgctgg ttctttaatt gttgcagtct tcttttattt
     5461 gcacgtaatt tctcttgttc taaacgaatt tttgcttttt gattttcgat ttcttgttgt
     5521 ttgtgtgttg tatttcgcat aggataaccc cttcattatt ttgtagtgcg gcgtgttctt
     5581 ttcttatagt tcgttttttt cttttcagaa aattcaaagc ctgttgtaaa gtcttctttt
     5641 aaaacgatcg ttgcttcaaa cgaacctttc tgacctttta gtcctctgac tgattggctt
     5701 gttcggccgt tttgaatgat ctctttcagt tgtgtggctg tgagtttctt tttggcaatc
     5761 gttgtccata atttaaaatc acaggcttca gacgtacatg ccgccacatt ttgataaagc
     5821 aagacttctt gttcccgaca ttttggacaa acacctaaat gacgcgttgc ttcctgtttt
     5881 tcactggctg ctttcacctc ttgtaacgtt gatccaaaat ctgttgactg gatttgtccc
     5941 ggtacgactt ccaacaagtg agagacaaat ttttggatat tagctaagaa agtagcttgt
     6001 gttcctttct gctcaccaat ctttaataag taactttccc atttggcggt catttcggca
     6061 cttgttagca gcggctcttt ttccacggct tggcaaagta aaatcccttt atttgtcacc
     6121 acaagcttat ttttttctac cttgatatat tccttttgtt tcaaagtttc aataatattt
     6181 gctcgtgttg cttctgtacc gattccttct acttctttca atatggattg tgcttcttcg
     6241 ctatccacgg tttttccagc tgttttcatc gctgtgatca acgtaccttc ggtataaggt
     6301 tgtggtggct tcgtttcttt ttcaaggact tgtagatcta ccgtcaccgc ttctcctttt
     6361 gtcactttag gaagtagcgg ttcttcttcc ttttcttttc tgtcgctctc tttgccaaac
     6421 agctttttcc aacctagatc tactggtact tttccgactg tctttagctg aagctgctga
     6481 acttgcgtca agatcgttgt ttcttcatag gtatactttt ccgcaaacat ggccaccgtt
     6541 gtttttaaca cttgttcata aatagcctgc tgcagtggac ttaattgttc aaaagccgct
     6601 gccgtcatga ccgtctttgt aggaatgatc gcgtaatgtt cctggacttt cttattattc
     6661 acatatcgac tattcggtgt atgaatagga gtaggaatgg cttcggcctt taaaaagtgt
     6721 ttgtattcgt ctaagtgatc caacagatac gcatactccc cttctgtaat ataaggggta
     6781 tctgttcgtg ggtagcttag atatttccct tcatacaacc cttgcatggc ttccaaagta
     6841 tctttggcac tagctttcat gagctgattc attttagatt gaaggctgga tagagaaaaa
     6901 agacgtggac ttgccgcact ttttttctcg gtttcaactt ggtggatcgt acctggctgt
     6961 tttcccaatt gaaccccctt ttcttttaaa taatcttcaa atgcttcttg cgtcgcttgg
     7021 gtttcattcg gatcaagctt cgcatcaaat gcgccatttt gagcgattac ttgggctttt
     7081 ccttcaaaat atggttcttt ttggaagttt ctaatctttt cctgtaattg ataaatcatg
     7141 taaagcgttg gggtttggac tctgcctaag gaaaaacttc ctggtatgcc tttttgctgc
     7201 aagagtaaac tatacagcgg gctaccgttc atgccgatca accaatcggc gatctggcgt
     7261 gcttgtgctt cttcaaattt tgggtaggtc tcttctgcat ctcgtaactg ctggaatccc
     7321 tgataaatgg cttctttttc taaggagttg atccaaagtc ttttaaatat ctttccatga
     7381 tcggcgcctg cttgttgaat gatcgtccaa gctatcagtt ccccttcacg atcgctatct
     7441 gtcgcaataa taatggtgtc ggccgattga agttgctgtt tcaccacctt gaattgcttt
     7501 ttcttatctt taggaacatg atagtgataa tgtgtaggga aaataggcaa atgctctaat
     7561 gaccattgtt tccaattttc atcgtacata tcgggcgaat ctaactcgac taaatgacca
     7621 aaaccatacg tgatcacagt ttctcctgaa aacaatcgat cttggatctc aaaataccca
     7681 tcttttcggg tagctttaga aaagctatcg gcataagcct tcgcttgtga cggtttttct
     7741 gctaaaataa cggttttcat catgacgatt tcactccctt tcttgctgtc taccttgttg
     7801 aaacaacatg gcacgttttt gctttctgat ctcttgttgg tagatttcat gcttcaattg
     7861 atcattccaa tcctttttta cttcaggtac aaattcatgt tcatgatttt tatagccttt
     7921 gtagtattgt tctgctttat aagagaaaag aatctgagca ttaatatccc ctttttcttg
     7981 ataaaaacga tcaaaatcgt acgtaccatc cacgcgttca caagctttta attcttttgc
     8041 gacttctcta caaatatctt tcaaattaat agcttctatt gaatcgttaa cagaaagctg
     8101 ctgaccaaaa gacgcattta ctttgtgagc ggaaaccaat tggttcgtta attgcagatt
     8161 gttttctgtc ttgtgtatgg ccataatagc ttctggtgtg atcttttctt cttttgcgac
     8221 ttcttcataa atgactttgt attcttttgg cacttgccaa tcattagcga ttcctcgttc
     8281 acaacgtact ttctgattcg taaatggatc aactgcgccc attaattgtt ctttttcgta
     8341 gaaaatatgt cctgcacggt cattatccac acaaatctcg atggattgag gaaaggcttg
     8401 tttctttctc aattcagata ctgcctcacc aaaataatga ctgatcacat ggtgttttaa
     8461 cccttccatc gaaactaacc acgtatcgtt taactgatca cgatttagtg cagcatagct
     8521 aagtaaatca atactactct caaaaaattt taactgctta gggtcaccga tctttaggtt
     8581 aaagccatga ttcgctgtcg aatttttatc tatatgctta tatgtgcctc tctcaccaaa
     8641 acgttcataa tcaatgaacg ttccttgata actcgcccct actgtctcat ttgtctgtgg
     8701 atctttccat aaaaaggcga ccgcatgatc taaacgatcg tctcgaacta agaaattttg
     8761 ccgttcatgt tttggcttta gttcggcaat caacccttct ttttctgcat aatcaatcag
     8821 ccactctggt agttttcgtt cttccttcag ataattcctt cctttttctg tgagtggttg
     8881 ataatcaacg gggtgatctt tgtacttttc tgtctcaaaa tgaagatttt ccactcgttc
     8941 tgttttgtct tcattcttta gaataaaatc agtggctgct ttaaattgtt gcttagggtc
     9001 ttggatctct ttgtctatga aaaaaagttt agctaattca ataatgtcgc cactaatatt
     9061 ttgactgttc caataaaaac gttgttttct tcgatcaaaa acaaatgaat catggtcttc
     9121 taagcgatag tctcgtcctt tattgactac agccattcca ttgtttcgag caaaatccac
     9181 tatatcgatc tgtttacatc gatcaataag actcattttt ttctcggtca ttttaaaacc
     9241 tcccaaaaga aaaaagacta tccgtttttc agatagtctt ttttacttgt tttatacacg
     9301 ttctttttgt gacaattgtt gttcataata gatttcattt tttcgttttg ccacttctga
     9361 cactgtctca agtgaaaatc ggaatgaatt tcctagcgca aattcttctg aaggtaacgc
     9421 cgtttggatc tcttttccat aactttttcc ttttaaaatt acttgattat gctgtggatc
     9481 ttcaataaac gccgcatcct tcaaatgtaa cgttcgaccc gtatcttctt ccattacttt
     9541 cccgtctttc ttgtcgatcc aaagtgaacc aacaggaaca atattctttt gttctgccgt
     9601 ttctacagta tttttatgct cctgcttcac ttctgctggt tggatctctg gttctctttc
     9661 tctgtattga ttgatttcat tagaaatggt ttgaatgaat tcatttgcga cacggctgac
     9721 atcattcatg attttcccac gttccgcagc ttccttatca tccaacgtct gaccattctt
     9781 cgtccatgct gcaatataag gaatagcttt ctccgaagtg tccattccaa aatgtttgca
     9841 cacaatataa gaagtcattt ctgcctgcaa ttctttagtt gcggtatcta attctgaaaa
     9901 tttgtgcatt cttgcatggg ctaattcatg aatacttgta gctaaatgct gcgtaggagt
     9961 atttcgagta ttcatgacga tttcttcttc gccagtaaat tcgttacgaa catatgcccc
    10021 ccgtgcttgt cctaattctc tttgatagac ttcactttct gtcatatctc gaatttgtat
    10081 tccaatattg ttggctaccg cttgaatccc agcttgaagt tctgactgtc cttttttatc
    10141 caattgaaaa tcaaagacac ggttcggaaa gattttcgga tagtcttcag gttttaactg
    10201 tgtttgtgtg atatcaaaca ctttttcaat cgtgtagtaa ggttttttga tcacttcgat
    10261 ttgaccactt tcgattcgtt ttttatcttc tggtgtagct tctgtttctc gaacaaagcc
    10321 acgtcctttc tcataaaatc ctttgaccgt ttttctgaca aagatcttgt tcccttgttc
    10381 cccttttttg atatagattc cttctttttt caacgcagca cgacctaaac aagccgttgc
    10441 acctggaaat tgttcctcaa tcaataattg atttctaaag gatcgttctg gaaaacgaga
    10501 cataaaattc aaaagttctt tcacgtcttc aggagaatgg gtatgctttt tcaccgcatc
    10561 cagtgcctgt tgcgaaaggg atttgacttc ttgcttaatc tcttctgccg ttcgtttttg
    10621 atagcccttt tgttttgttt ttgtttcttt cgactgcatt tgttctttcg aaacaggttc
    10681 atttttgacc gtttgttttt gttccttttc aggtacttgt tcgcacaatt ttgctaactc
    10741 taaatagatg ggttgattac tgttgacgcc atcccctaga tccattcgga tctgatcgac
    10801 tacttctccg ttcttcactt cttcaaaata gaatttataa taacctgctt ctgcggcgta
    10861 ttggttatcc aacgttttga gttgatcgat caattctaat gttacgatct ccccaccata
    10921 acttttcaat cctttttctg gatcattttc gttgaattct actcgataga aatgatcgtt
    10981 ttctttctcc ttcaaagaat cagttggcgc agcctcttca agtaattcac gaacagctgc
    11041 tttttcctct tccgacgtgg cttggtattt ttttgattcc tcaaatcccc cgttttcatt
    11101 cagttctaaa gcaacttttc gatagtcttt caaaaaataa atggttggat tttttttcca
    11161 ttgcgtcact cgtccgctgt caataattgc ttgtacttca ggaagaagat tttcttttcc
    11221 tttttctgat ctttctttca gctgttctaa tgtttccaat ttccgttggt attttcggat
    11281 cgtagcggct gaaaaagtcg attcaccttt ttcaaagcgt tcgatttctt ctttgattct
    11341 tggaatattt tcgattgtca tatccaggcc gccgtacttg ttatagccca tttcctttag
    11401 ctcttcttgt ctttcaagtt tttctacctg tttttcttgt tgcttgatct cttctctcag
    11461 actatgcact ttgttttcta aacgttcttg ttgcctaaac caagaatgcc catttctctt
    11521 atcgttcatt ggctgaccgt tggtctttct ctggtgttca aacatcttct caatttgttc
    11581 gtttaattca ttttgcaatc gtactaattt tcttttggat tttcccaagc gactattgtc
    11641 tagtggttct ttttcttctg ccatgtc
//
LOCUS       DAYYCY010000080        11364 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000080 DAYYCY010000000
VERSION     DAYYCY010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.080"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..511)
                     /locus_tag="ACHAZB_001717"
     CDS             complement(<1..511)
                     /locus_tag="ACHAZB_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFM8614364.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALR"
     gene            complement(523..1215)
                     /locus_tag="ACHAZB_001718"
     CDS             complement(523..1215)
                     /locus_tag="ACHAZB_001718"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFM8614365.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYKPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1234..1518)
                     /gene="macP"
                     /locus_tag="ACHAZB_001719"
     CDS             complement(1234..1518)
                     /gene="macP"
                     /locus_tag="ACHAZB_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall synthase accessory phosphoprotein
                     MacP"
                     /protein_id="HFM8614366.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1531..2094)
                     /locus_tag="ACHAZB_001720"
     CDS             complement(1531..2094)
                     /locus_tag="ACHAZB_001720"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFM8614367.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2322..2984
                     /locus_tag="ACHAZB_001721"
     CDS             2322..2984
                     /locus_tag="ACHAZB_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HFM8614368.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3108..4304
                     /locus_tag="ACHAZB_001722"
     CDS             3108..4304
                     /locus_tag="ACHAZB_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HFM8614369.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4405..5781)
                     /locus_tag="ACHAZB_001723"
     CDS             complement(4405..5781)
                     /locus_tag="ACHAZB_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8614370.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5818..6684)
                     /locus_tag="ACHAZB_001724"
     CDS             complement(5818..6684)
                     /locus_tag="ACHAZB_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFM8614371.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6773..7798)
                     /locus_tag="ACHAZB_001725"
     CDS             complement(6773..7798)
                     /locus_tag="ACHAZB_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM8614372.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7816..8490)
                     /locus_tag="ACHAZB_001726"
     CDS             complement(7816..8490)
                     /locus_tag="ACHAZB_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8614373.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8674..9567)
                     /locus_tag="ACHAZB_001727"
     CDS             complement(8674..9567)
                     /locus_tag="ACHAZB_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFM8614374.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9641..11338)
                     /locus_tag="ACHAZB_001728"
     CDS             complement(9641..11338)
                     /locus_tag="ACHAZB_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFM8614375.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
BASE COUNT         3369 a         2505 c         1955 g         3535 t
ORIGIN      
        1 gtctcaatgc ccattcatgt ccaacgggat cgaaacttgc cacggcatct ttgaaaatat
       61 ggagtttgta tcctagatta tacgcatcga ctgctgtatg caacacacag atatctgtac
      121 atacgccggt caaataaata tctgtgatct ggcgttctcg caaacggatg tctagatctg
      181 ttccactaaa agcggaataa tgccgtttgt ctatccaata aacattttcc tcttgctcat
      241 agtcttggta taaacttttt aaagagccgt acaattcccg tccacccgtt cctatcacat
      301 tatgcggagg aaataagcgg ttctccggat gaaaggaatc ttttggatca tgcgcatcaa
      361 tggcaaaaac gacaaaatcc ttttgatcaa taaacttctt tgtatgagca gtcagctttt
      421 tttcaattac ttgtcctgct gtcccagtag tcagctttcc atcctctgca acaaaatcat
      481 atgtgtagtc aatcgagatc aatgcgttca tcttagtcct ccttatacga gatgtttgac
      541 aaattcgatc accatttctg ctgatcgttt gcctgcgtct tcgataaatt cgtcaaagct
      601 ttgtgtcgcc gagtgatctg ctgtgtcgct cattgcacgt acgatcaaaa atgggatatt
      661 gaactgacga gctgtttgtc caacagcggc tccttccatt tcgcaagcta atgcttcagg
      721 gaaattcgtt aagatttctt tgattttatc tggcgagtcg acaaatgtat cacctgtaac
      781 gatcaatcct tcttttgcgt tcaaattcgt tgcttttgct gctttaacca tttcactgcg
      841 caaatatgtg cttgcttcat aatatagagg cattcctggt aattgtcctg gtttgtagcc
      901 aaatcctgtt gcatctgcat caaaataagc tactttgtct gatataacga tgtcacctac
      961 ttgtaagcct tcaccgattc cgccagctga acctgtattg atcaccatgt tcacgccgta
     1021 ttgttggatc aacagacttg tggtaataga tgctaagact tttcctatac cggaacgtac
     1081 gacgatcact tcgtggttac ctaatgaacc tgaaataaat aaagcgcctg cgcgttccca
     1141 tgataatggt tcgcttaggt tctctctcaa aatttttact tcttcttcca ttgcgccgat
     1201 aataccgatt ttcatctttt taattccttc tttctatata aatgcgattg ctaaaaaaac
     1261 cacacataac attaggataa caataataag agatttcatc aaaaaagagt tccatttggt
     1321 cgtcttttcc cgttcgctga tccttgtttt cgtgattggt ttattctttt tgctttcttt
     1381 acgataaaag ctggcaatct ttttttcttc ttgctgataa gctgtttctg cttttcgctg
     1441 tttttttaat gactcgcttg cttgtgcttg ttgtcgcttg cgaagttcgc ttcgcgtaat
     1501 aagcggtcct ttggccataa gccgatccct ctactttcct ttggtcaata gttcccagta
     1561 ttgaatcgca taaatcgtct ttgcatcaca aatcaactga tctttcatcg cttgttgcgc
     1621 ctcttcaaga gttagatgat acaactccag tacttcgtct tcatcttgtg ctaaaggatt
     1681 ctcgaccttc tctacccctt gtgcatgata aatatgaagg acttcatttg caaaacctgg
     1741 tgaaagatac atcgaagtaa gatggctcag cgacttcgca cgatagccag tttcttcttc
     1801 taattctcta gctgctgtca tttcgggatt ttgtccttct cctggatcga tttttccagc
     1861 tggaatctcc aaaatcactt tttctaaagg ttttcgaaat tgtttgacaa gaagcagacg
     1921 atcttgttcg tcaaaagcaa tgatcccgac accaccagga tgaaatacca gttctctctt
     1981 cgaagttcct ccatctggca aacgaacttc gtctacagca acatcaataa tttttccgct
     2041 atagatttct ttgcgactga ttgttttttc ttcaaattgc tcattgttca gcatatcttc
     2101 tcctgctttc tttgtaatca taactgcttt tttttcattt ttcaattttt ctcaaaaact
     2161 ccttattagt ttaccataga taaaccttag attttctcta aatatagaaa atccttcttt
     2221 agtccgatac tctttctagt agtccgtatg ctaaaataag tatgtaatcg aataacgaaa
     2281 agaattcaat actcaacgat aaatgacatg gagggtttat tatgataaaa cgaaattggc
     2341 cgtggcttct agcattggtc ttgctatttc tctttgtccg aaacaatagt ttttcttttg
     2401 ttctaggact tttattgata ggtgtagttt tagttttgtg gggactcttt ggttcgcgca
     2461 ggaaaaaggg aaaacaagaa gaaattcctg cactgactga cgaacttgaa actcactatg
     2521 aaaaaagcgg tatgacagcg aatgaaattt cattttttcg acaaacaatg aatcaaacaa
     2581 aaaatgagat caatcaattg caaatgaata tgcagcagac agccaaactt aaagcaatcg
     2641 atctccgcca tgatccagtc aaagcagcga aagctttatt caaagaattg gtgaaagaac
     2701 caaaccgact gcatgaagca agtcagtttc tttatacgca tctgccgaat ctagttgatt
     2761 tgacaaataa gtataatgag atcaatgacc atgagatcaa aaacaaacag acctatgaaa
     2821 aactagaaga aagcgcgcag atcatcgacc agctgtcagc tttaatcgca caagattatc
     2881 aaaaattcgt tgctgaggat ctagatgata tcgacgtaga gatatctgtt gcgaaacaga
     2941 gcttaaagcg tgataataaa tatgaaccag aacaaaagga ttaactcttt tgttcataaa
     3001 agaagactcg gaacattcag atgatgtctg gctgtttcga gtactttttc taaagtagag
     3061 acattcatgt aagaatcgag aacagattca acaaggagga attttttatg gatcataaac
     3121 cgactgaaac aaaagatgtt acaccagtta acgatacact tgaagattta ttgaacaacc
     3181 cgttttcaac tccaacagat acgctgacca cttcacaaca agcggagatt gatcagctcc
     3241 aagagcaaca gacagctgct cgtttaattg acaaactacc ggcggaacgc caagaacaag
     3301 ctaaacagct agcagcaaaa atcgatgcta atgatgcaca gtctgtcatc agctacggat
     3361 cggctgccca agcaaaatta agtgaattct cacaatcgat gttgaatcat gtgcaagcac
     3421 aagatattgg tcctgtagga gattccttaa ctgaactgat gtatcgtctc caagaagcca
     3481 atcctgacga actgcgtgcc ggagaaggga acatcttcca gcgtgtcttc gggaaggtca
     3541 aacaatcgat ctatgaagtg acagcaaaat atcaaaagat tggtgcacaa attgacaaga
     3601 tttcagtgaa attagataaa gaaaaagacg gtctgctaaa agataatctg atgttagaac
     3661 agctttacca gaaaaacaaa gactactttg atgcactgaa tatttatatc gctgctggag
     3721 aattgaaaat ggaagaactt caaacaacga tcattccaga agcgatgaaa agagcagaag
     3781 aaactggcga tcagatggat gttcagattg ccaatgatta cacgcaattc ctagatcgtc
     3841 tggataaacg tacgcatgat cttcgcttag ctagacagat cacgatccag caagcaccac
     3901 aaatccgttt gatccaaaac acaaatcagg ccttggctga aaagatccaa gcatctatcg
     3961 ctacagccat tccgctgtgg aaaaaccaag tcgtgatcgc attgaccttg cttcgtcaaa
     4021 aagatgcagt aactgctcaa cgccaagtct ctgaaacaac aaacgactta ttgaagaaaa
     4081 attcagaaat gctgaaaatc tcagccatcg aaacagcgaa agaaaatgaa cgtggaatcg
     4141 tggatatcga gacccttcaa accactcaaa atgatttgat cgaaacaatc caagagacat
     4201 tgcgtatcca aaaagaagga aaagaaaaac gtcgtcatgc agaagtagag cttggacaca
     4261 tggaagaaga tctgaaacaa aaactgttgg atttaaccca atagatcacc tttcgcttgt
     4321 tactaaaaca aacgcccaac aacagtttat tacaactgtt gatgggcgtt tttcttatct
     4381 ttcttctaac ttctccattg gagtctattt ctcattcatc ttgctgtgtc catagccata
     4441 gccaaaataa atgatcagtc caataaccat tacgataccg aacacgatcc atgtcacagt
     4501 ttgcagacga agcatcaata caatacaagt caagatggag acgattggca atagcggaac
     4561 gaatggcact ttgaattctc cagcttttgg tttgccaaac atttttctca acttcagtaa
     4621 gcctagacta acaagcatca aataggcgag cgccatgata ttcgttagtt ccgctaatac
     4681 attcaatggg aaaaatccgg caaagaatac agctgccaca ccagcggcat aagtcgcttt
     4741 tttcggtgta cgatgctttt gatcgatttc tgtcataaac ttaggaagta agccatcctt
     4801 gctgacagca aacagtaaac gagcaagtga atacatcatc gagatcgtta ccgtcagtag
     4861 cgttaaaatt gcccctacag cgatcaccgc gcccactgtt ccatgtccta catacctcat
     4921 tgcaaaagcc actgggtcat tgactcctag ttcagtatac ggaacgattc ctgtcaagat
     4981 cagcgtcacg atgatgtaaa ggacagtagc aatcagtatc gaaccaatga ttcctcttgg
     5041 cacatctttt tgcgggtttt tgacttcctc ggctgccatt gcaacggcat caaatcccaa
     5101 gaaagcaaaa aacacaagag ctgcaccatc tagcacaccg gaaaaaccaa aaggcatgaa
     5161 tggctgccag ttatcaggtt taacgaaaaa tattcctaca atgataaaca acgcgatgat
     5221 accaaatttg acatatacca tggcattatt caaacgtaat gctttcttgg cttcaagcga
     5281 cacgatgtaa gtgacgaata tcactacgag caccgcaatc aagtcaatgt atgtaccatt
     5341 ttccggattg tacccgccag ttaatgcttg tggaagttcg gcccctaaac ttttcaaaaa
     5401 accttgcata tagccagacc atccggaagc aacagaggaa accgctagta aaaattcccc
     5461 gatcaacaac caccctgtca gccaggcagc aaattcacca aatacaacat acagataggc
     5521 atatggccca ccaatcactg gcactcgcga agcaaattct gcgaaactta gtccagacaa
     5581 aatgacaacg attgcagcta agacaaatga aagtgacagt gctggaccag cattttgatt
     5641 ggccgcaacc cctgtcacca caaatattcc cgtaccgatg atcgcaccaa ttccaagcat
     5701 gatcaaatca gacgttttta gttctttttt catctcactc gtatgattga gattgatctc
     5761 tttcttacga aacaaatcca ttgaagttcc tccccatttt aggttggaaa acagcgatta
     5821 aactgttttc atatacccta aaaggatacc acctaaaatg actaaaaggc aaccaacaat
     5881 aacataaatg agctcttttt tcgtttttct ttcgccaagg atgaaaatcc cacctaaagt
     5941 agagataatg atccccattt gtgaaagtga gaagccaaca gctaaaccaa gtgatttgac
     6001 tgttagaagc atgcagatgt ttcctaagcc ccataataat ccacagatca tgtttttcca
     6061 cacaaactga tcgactttaa ctttacggaa agcaaagaag cttgcgccta agatcattcc
     6121 gatactttga ggtaagataa ttgacatcgc atccaaattc gcccagttga cgatgatggt
     6181 atatacacca taaccaatcg tagagaagat caatgctcta aaacccttac caaaatcaag
     6241 cattttatta tctgtatcta tttttccttc gtcatcttgt cgggcagtca aatacgcacc
     6301 gcttacaaga agagctaaag caataatacc gtatacataa tctttcgttt gtgtccattc
     6361 atggaagaaa acggctcccg caatcgtatt caaaattaat tgaaaaccag tagacaatgg
     6421 tagccctaca gaaacaccca tgtacttcat tccatggaac tgtccatttt gtccaacact
     6481 ccaagctaag ccggataaaa atccgatcaa gaaaatccaa ggggtgatta caggactaac
     6541 aacgaaaaag acaactaaag aaaacagaaa tgcaccgatc gtcattccta gtgtttgttg
     6601 attggcatcg ccaccaattt taccacttac taaaccaata ctaccccatg ccaacatagg
     6661 gactaatgct attaaaattt ccataaccaa tctcctttta tttcacatga taagaaactt
     6721 atcagcgaaa acgttaacaa cacaagcact ttttttcgtt tctaacgatt ttctaacgtt
     6781 tttcattata aaaatttaag attgggaaaa ataaagtcat tgttgtccct tttccttcag
     6841 ttgattcagc cgtcaaatgg atacctaatt tttcggccag attttttgct aaataaagtc
     6901 ctaatcctgt tgaatgtgtt ttgctaagtc gaccatttct cccagtaaat cccttatcaa
     6961 agatccgttt caagtcttcc gctgggatac cgatccctga atctttaact gaaaggctga
     7021 ctccttgcgc ttccttttct atgctcacaa tgatgtttcc gtgatccggc gtatatttga
     7081 tggcattact aagcaattgg ttgaagataa aagcgatcca tttagcatcg gtcaaaacag
     7141 tatgatcctc accaacgatc tcgtagcgaa tatttttttg gataaaataa tttgcctgac
     7201 tgcgtatcac aggctgaatg attcctttta gtccatattc ttggatgagg tagtcccgcg
     7261 agaaactgtc gagtcttgca taatacaaaa cttgttctac gtattcatcg atttttgata
     7321 attcattttc tagtaatgta tacttttgat cgtcaatatc gaactcgatc gaacgaacta
     7381 gcaatttaga agcggaaagt ggcactttga tttcatggac ccatgagtca atataatctt
     7441 tttggtcttg ttgattactg attgctgatt gcattacttc ttggtgttca cggatcagct
     7501 gattgatata ttcttctacc agtttttctt cttctgtcct cccaccactc aaataatttt
     7561 gcaaagggga agttttttgt atgtcggcta attttcccca ccatcttctc ttgctcaagt
     7621 aaccacccag cagataaaaa atcagaaaga cgccctcaat caaagctaaa taagcaattg
     7681 tgctcaaatc aactttgatg tttggcgcca gccacatcac aaagatcgtc aatgcaataa
     7741 aaaacagcca tcccaagatc aatgtccact gatctttcaa atatttaaaa aaattcatct
     7801 taaccgctca tcctttcagg gaacgatata tccctgtcct acttttgttt cgatatatcc
     7861 ttggatacct gcctgttcga tttttcggcg taaccgattg atgttgactg tcaatgtatt
     7921 gtcatctaca aatcgttcgt catcccacag tgctcgcagc aatttttccc gacttaagat
     7981 ctttccattg tgcttcatca aaataaataa tagacgatat tcatttttgc tcaaatcaat
     8041 gatctctccg ttgatttcca tacttccgtt gtcaacattc aacgtaatgc cattgtggct
     8101 catcatttca ctagataatt ctgtgtagtt gtaagaacgg cgaagtaatg cattgatttt
     8161 tgcaatcagt acatcgattt ggaaatgttt tgtcacaaaa tcatctgccc ccatgttcat
     8221 agccatgatc atatccatat tcgtattcct gctggagata aaaatgatcg gtactttcga
     8281 tacttcacgt attttttgac accaataata gccatccaaa acgggaagat taatatctag
     8341 taatacaagc tgtggttctt ctctttcaaa gtctgagaac acttgattga aatcggtcgt
     8401 tccaaaggtt tcaaattgcc atttctcaag ttcctccatg atcaattgtc gaatgatctc
     8461 ttcgtcttcg actaccatga tttttgccat aaaaaatccc tccatctttc ttcttgctac
     8521 tacaatatca aagaagccct tgtgaaacaa gttggcttac ctggaaaatg tcattttagc
     8581 aaacgttaaa aaaaactgga aatatgcgat ttaatcgcgt ttgtctcttc caagcagctt
     8641 aaatttatta tgttattctt aataattggt ctattatctg ttatttttct ttcgttgttt
     8701 ttgtctttta aattttcgat aacaccaata accgaaaagc gttagcacta gcaatatacc
     8761 tagaaggagt ataagtaatc gataaagatg gtattttttt gtctgttcta tctttgaaga
     8821 gacttcttct gtaaagggaa ctcgtcttcc tgtcactagt aatcggtgag tgttcacggt
     8881 gtaaggtgta caagtgataa gtgtgactag atcctgcttt tctctaattg ctaatgtatt
     8941 tgtttttgtt ggaaggacaa caattttctc aacgacttca taagcgagca tccgaccata
     9001 cacttcaaga tagaatcggt catcttgctc catttcttca agatccgtaa agagtttttt
     9061 ctcagcaata ccgctatggg cggtaatcac ggaatgtgta ctctctcctc cagttggaaa
     9121 agaggtgcct tgcaggactg tcgctcctct atctaacaaa agatcattcg tttcatcaaa
     9181 taaaggcaag ctaacattga ttttcggaat atagatggct ccgatcatat gttgttcgta
     9241 atattctttt cctggatttt ggacatttct aaccgcttgc tcaaaaggat ctttgacttg
     9301 tcccattcct gggattactg atgtatgagc aagcgtctga tttttctttt catgcactgc
     9361 taattgttgc tcatgttttt cttctttatc gcgttcaagc tgcttttgat aattctcgat
     9421 ccttctttga tccaaataat tgctgagagc atctgctaaa aatggatatg taaaaactgc
     9481 agctccggca agaaacatca ctgccataaa taattgtaat aaaagactga tttgggtttg
     9541 aggtctatta cctattttct ttttcatttt cttcaccttc ttatttaaaa aaactgcagc
     9601 aacctttata aaaccgattg ctgcagctta tttttgttgc ttatacttca gacttcattt
     9661 tagatttacg gtaccagctg tacgctccaa tcatcaaact tattccgatt agtaagaatg
     9721 ccaagagccc gttcccacca gtagcaggaa gtgacccgcg tttcatattc ggtactcttt
     9781 ctggccctgg attttctact cctacgatct gaatttcttc attatagctt ccataagaga
     9841 tgataaattc agtaaaggct gcctcacctg ttggaagtac ataaccttct ggcgccatgg
     9901 tctctttcat ttgataggtt ccataatcta acccttgaac ctcaaagtat ccatttccat
     9961 ctgcgacaaa ttctgttgct tgtccgcttg ttgcccactc aattctatct gctcctgatt
    10021 cataaacagc taaaggcgac tcccctttat aaaatattgc gtattcagaa cggtcttcat
    10081 tccacaattc gaatcttgca tctttaagca attcactgct tgatgcatca tatttttcaa
    10141 actttcttcc acctgttgtg actttctctg gcggctctac tccttcttca taatcaaatt
    10201 catcatttcg tccgatttca ataaccgctg tgttgttatg gaaatcatcc gcttctagtt
    10261 ctgtggaaac cttcaaatta tacgtgatcg ttaaatctcc tccacgtgca tctgctaatc
    10321 tacctaaagt atctactttg gctactccat tttcttctgt caaaatcgtc acgacaaacc
    10381 cgttgcttac tacttctact atataatctt gtccttttgt taaaatcgtc tcgccggctc
    10441 gcacttctat ttcctgatca taataagtta gaccaactgt aggcgtatct gtgattttgt
    10501 aatttttgat aacagcgctt ccgttttcaa ggacagaatc gatgaaataa ggaataggtg
    10561 ctgtgatcgt gtaagaagcc gtttttcctc tttctagatc aatataagaa atcgtttctt
    10621 gttctccagc ttcattaata atcgttactt gtctaaggtc gctttcctct acattcatct
    10681 gtttatctgc ttctctgatt tcgtttttcg gatacaaatg cacatctgta ttgtatgtgt
    10741 ctttccatgt agaaccatcc cacacgccat ccaccatatc aggcatcatc actggcatca
    10801 taacaaccat tggagacgcg attctggaaa tatgagctgg cgaataagtt tctaagaaaa
    10861 gatagacttt atctctagaa gaaggtgctt cttccgtcac ttggacggta gagaataccg
    10921 cttctccagc ttcattcgtt gtttgtttgt caatacttgt cagtcgatat tttaaaaacc
    10981 aactttctgt gtgccgtttt tgaatccatt caatcgcttc ggcagaatta agaccgtctt
    11041 ctcgttccgg tgttaaagga ttatctcgaa tcaaatcgta atatacttcc gttacgtcaa
    11101 acaaatcaaa atcgactcct ggaatatttt cttcaccaaa atccgtagaa accaatccgc
    11161 tattcggtcg ttcttccgga acagatgaga aacctttttt gtgtaatgta atcgtcactt
    11221 cacttggttt ttgtgcgact tcttcggctt tgactagttt tccgttcccg aaaatcccta
    11281 ttcccgcagt aacgagtaac agtacactca ttcccgtgac aatcattttt ttattcattt
    11341 ttttctcccc gttctttttt tatt
//
LOCUS       DAYYCY010000081        11278 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000081 DAYYCY010000000
VERSION     DAYYCY010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11278
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.081"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            22..1653
                     /locus_tag="ACHAZB_001729"
     CDS             22..1653
                     /locus_tag="ACHAZB_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HFM8614376.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(1715..2356)
                     /locus_tag="ACHAZB_001730"
     CDS             complement(1715..2356)
                     /locus_tag="ACHAZB_001730"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFM8614377.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    2542..2618
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            2638..2946
                     /gene="rplU"
                     /locus_tag="ACHAZB_001731"
     CDS             2638..2946
                     /gene="rplU"
                     /locus_tag="ACHAZB_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFM8614378.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            2973..3317
                     /locus_tag="ACHAZB_001732"
     CDS             2973..3317
                     /locus_tag="ACHAZB_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HFM8614379.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            3330..3623
                     /gene="rpmA"
                     /locus_tag="ACHAZB_001733"
     CDS             3330..3623
                     /gene="rpmA"
                     /locus_tag="ACHAZB_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFM8614380.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            4057..6864
                     /locus_tag="ACHAZB_001734"
     CDS             4057..6864
                     /locus_tag="ACHAZB_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM8614381.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            7055..7546
                     /locus_tag="ACHAZB_001735"
     CDS             7055..7546
                     /locus_tag="ACHAZB_001735"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFM8614382.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            7649..8638
                     /locus_tag="ACHAZB_001736"
     CDS             7649..8638
                     /locus_tag="ACHAZB_001736"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFM8614383.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            8671..9474
                     /locus_tag="ACHAZB_001737"
     CDS             8671..9474
                     /locus_tag="ACHAZB_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFM8614384.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            9495..10409
                     /locus_tag="ACHAZB_001738"
     CDS             9495..10409
                     /locus_tag="ACHAZB_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8614385.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            10492..10866
                     /locus_tag="ACHAZB_001739"
     CDS             10492..10866
                     /locus_tag="ACHAZB_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HFM8614386.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            11005..11250
                     /locus_tag="ACHAZB_001740"
     CDS             11005..11250
                     /locus_tag="ACHAZB_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFM8614387.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
BASE COUNT         3550 a         1913 c         2493 g         3322 t
ORIGIN      
        1 catgaaaaag gagttagata ggtgttcaaa aattatgttt cctcactaaa aaaagaattt
       61 tcaggttatg atctttcaag atttcagaaa gatcttttgg ctgggatcac agtagctgct
      121 gtagcattgc ctttagcctt ggcttttggg gtatcaagcg gagctgatgc ggccgcaggt
      181 ttgattacgg ctatcattgc cggtatcgtt attggcggtt tatcaggcgg tttttatcaa
      241 atatcaggtc cgacaggtgc gatggcagct atcttgatgt ctattgcggc aacaaaggga
      301 atgcaaggtg tattgcttgc tactttttta gcgggtctat ttttacttgc agcaggactt
      361 ttccgattag gaacactgac ttcttttatc ccagcacccg taattacggg gtttacttca
      421 ggtattgcta tcattattgc attaggtcag atcgataatt tatttggtgt tcactcggaa
      481 ggtacaaacg ttattgaaaa actaagtagt tatcaaacga taggatttcc gataaacaca
      541 gcctctttga tcatgggagg gctagtcatt ttagggatgt ttatctatcc aaaaaaatgg
      601 gcgaaacgaa tgcctagttc gctgctggct atcattttgg caacagcagt tatgctattg
      661 ttccatttac cagtagcaac tgtgggtaag attccacaaa cattgatcag tagtaatcgt
      721 ttgaatatgg gagattttag cttttctgcc ttgcaacaag tggcagttcc agctattagc
      781 attgctttat taggaatgat tgaaagcttg ctatgtggtg cttcggctgg ccgaatggca
      841 aataagccct tagatagcaa tcaagaatta atggcgcaag gaattggaaa tctgttgctt
      901 cctttctttg gcgggattcc tgctactgcg gccatcgccc gtacaagcgt ggccatcaaa
      961 tctggtgcac aaactagatt agctggtatg atacatgcag ttgtcttact gctctccatg
     1021 cttgtttttg cgccagtgat gtcacagatt ccaatgccag cacttgcagg tgttttgatt
     1081 gtgacggctt ggcgtatgaa tgaatgggaa acgatcaagg agctatttac taataaatat
     1141 tggtcagcag ttctattgtt tatccttacg atgatttgta cagttatctt tgatttaagt
     1201 attgctatcg tgatcggggt gatcggcggc tgtgtatttt ttgttgtcaa aagcgctgct
     1261 atcacgattt cattagaaac gatcgattgg caaagaatgg ggctgccaga aacggaaaag
     1321 ctgaataact ggacagtagt gtatattagt ggtccactgt tttttatgtc ttcagaaaaa
     1381 ctcaaaaata aattggaaga actaaatcat cacgatggtg tcattttctc catgcgaggc
     1441 gtaccaagta ttgatttgac ggcacgtgca gtttttgatg aatttcaaga aaaagcagag
     1501 caaagaggac aaacgattat ctatacttcc ttgcagccag aagtagaaaa acaactttct
     1561 cacttgtggg aacatacatt cacgcatcaa catcaaacag ttgctcatgc gttgacagct
     1621 ttacatgcga aatatactgc aattagccat taaaacaatg aaaaagaaca gacaagactg
     1681 taaactgaag attttatggt cttgtctgtt ctttttaata ttgctgtaaa agtgaagcta
     1741 gtacttgcca gtcttgatgg ataacaggta caagggagcg attgtagaaa atagctgctg
     1801 gatgaaaaag tggaagaatt cgatagtttt tttctgacca ttcgtaggca tccttttgtt
     1861 ctgttaattg taagacaggc cctgtataga gttgaccatg atttttggta accgtatatt
     1921 ctttgccaag taaacgttga agaccaatgt ttccaagtgt cgcaattaat tttggctgga
     1981 ccttcatcag ttcgtaatcc aatattggtg catgcgcaaa tacttcggaa cgagtaggtg
     2041 tccgatttgg ataaacagtt tctgactgtt gtgtccgttt attgatccga tgagtcacgc
     2101 gatacgggcg gctccttaca gcactggtga tatacacctc ttctcgtgtt aagccagttg
     2161 aagaaagtgc ctgcatcaat tcttttccag cttgaccgct aaaaggaaca tgcgtatcaa
     2221 tctctgtttt ccctggggct tctcccacga gcatcagttt aggatgaaga ggtccttgcc
     2281 cagcgataaa tccttctaca ggacggccgt tcattcttaa agaagcttct tgtatcagtt
     2341 cttttgggta ttccatttat gctcctcctt tttcttttat tataagaata aaagaaacac
     2401 aatgcgacta ttttatttgg tagaactgtg aggatagaaa agcagaagta ttgcttgaat
     2461 ttttgtttta ctttttgatt ttaaatttag ttgactaacg gttgctgttc tggtaagcta
     2521 gtactgttgt aattgtggac tccacagcta caaccgctca gaacaagtaa taagcacagc
     2581 atatgctgtc acttcggatg gcgagtctaa gtatatcaga aggaggtgct ttatagcatg
     2641 tacgcaatta ttaaaactgg tggtaaacaa gtaaaagttg aagtgggaca agcaatctat
     2701 gttgaaaaac ttgacgttga agcaggagaa aaagttgttt ttgacgaagt tatcttagta
     2761 ggcggagaat ctacaaaagt aggcgctcca actgttgcag gtgcaactgt tgaaggtact
     2821 gttgaaaaac acggcaaaca aaagaaagtc gttactttca aatacaaacc taaaaaacat
     2881 tcacaccgta aacaaggtca tcgtcaacca tatacaaaag tggttatcga cgcgatcaac
     2941 gcttaatagc gtttgagaaa gaaggcctat atatgatcaa aggaacattt aagcgaaatg
     3001 acgcaggtca gatcgtttca ttcactttga ccgggcatgc tgatgctggt ccttatggaa
     3061 gtgatatcgt ttgtgcgggt gtttctgcgt tagcgatcag cactgtcaac gggattgcct
     3121 cattagccgg ttttgagccg attgttgaaa tgaatgaaga agaaggcggt tatctttata
     3181 ccgaagtcac ttcaggaatg acacaagaac aaaacaatat tgctgagatt ctattagaaa
     3241 atcttttatt aggattacaa tcgatagaag ctgaaaactc agaatatatc caaattaaaa
     3301 caattaatga taaataggag gtgcagacca tgttgttatc tatgaatcta caattatttg
     3361 cccacaaaaa aggtggcggt tctacatcta acggccgtga ctcagaatca aaacgtctag
     3421 gtgctaaacg tgctgatgga caaacagtta caggtggatc aattctttac cgtcaacgcg
     3481 gtacaaaaat ctatccaggt gtaaacgtgg gtatcggtgg agacgacact ttatttgcaa
     3541 aagttgatgg cgtagtacgt ttcgaacgta aaggccgtaa caaaaaacaa gtgtctgttt
     3601 atccagtagc tcaagaagct taattgaatc ttatgattaa agaggttgtg acaagacttt
     3661 tgtcacagcc ttttttgttt ataaaaataa aagttgtagt ggaggctacg aagtttactt
     3721 tgtcttttta cacttaatat ttttttgcca atttggaatc aggatgtttt gataactatc
     3781 gttttcaatt gatggaagct atattgtttg ctatcagtga aataattata tggtaaacag
     3841 tgttcttttt cttgtttcca ttataaaaat cgtatcggga tttttcgttc ttttaaattg
     3901 aacatttcta tctatccgcg tgttatcaac aaaacaaaat caggtaaata gttgacgaaa
     3961 cagaaataaa tacatagtat tatgttttga agcctaagta caatagtcta tagaaaagga
     4021 gcgataccat tggcaaaacg cattgaccga atttatatgt atgtcaaaga aaaaaccgct
     4081 catttaacaa aggcagaata tgatcaagga gtaacaacac aggaaattgc tgaagtgtta
     4141 gggattcagc gaacaaatag cagtaaagat ctgaatcagc tagtgcgcga aggaaaactg
     4201 ataaagacag acggacgacc agtacgttac atatatcaaa cactcgttac gcacgtaaag
     4261 ccagttacaa aacatgtagt cagttacaaa gaaacgccgg tctctgaaga aaaaaataca
     4321 ctccaaatag atactaagga tatctttgca aaaatcattg gagctaacgg cagcatgaaa
     4381 aattcagtgg aacaggcaaa agctgcgatt ttgtatccgc caagaggatt aaattgtttg
     4441 attacaggac ctaccggatc aggaaagact tattttgcac acgcgatgta tcattttgca
     4501 aaagcgcatc atgtaataga tgaggaaacg gaactgattg ttttcaattg tgctgattat
     4561 gccaataacc ctgagctttt gatgagtcat ttatttgggt acgcaaaagg tgcttttaca
     4621 ggcgcagatg aagaaaaaat ggggattatc gatcaagcgg atggcggtat gctgtttttg
     4681 gatgagattc accggttgcc gcctgaaggt caagaaatga tcttttactt tatggatcat
     4741 ggaacttaca gtcgtcttgg ggaaacaacg aaatcccatg aagcaaatgt acggatcgta
     4801 ggtgcgacca cagaagaccc aggttcttct ctattagaaa cttttgttcg gcgtatccca
     4861 atcaatatca aacttccggc ttttgaaaaa cgtcctgcaa atgagaaagt cgatttagta
     4921 aaaatcatga ttgcccacga agcaaaccgc acgcagcgaa agatttcttt gacagaagat
     4981 gtagcaaaag cattgatcgg cagtgtgacc tatggaaata taggacaatt gaagtcaaat
     5041 atccagttag tatgtgcacg aggatttcta aatcatatgc aatcacccga aatctcgatc
     5101 acaatcgatg atttgacaga agggattcgt agcggattga ttcaattagc aagcaaccgc
     5161 agtgcaatgg cagagctttc taaaatattg gagccaaaga taactgtagc accaaatgat
     5221 acgatcatga aaatccaatc ggactcctat gagttacctt ataatctata tgatatcatc
     5281 ggagataaag cagctttact gaagtcggat ggtttggatc aagaagcaat caatcatttc
     5341 atttctacag atatcaacat ccatctgaag tctttctata aagaccatgg tttttctttt
     5401 aatgcagaca gcaaattagc agaatttgta gatccgaaaa tcatagaagt gaccaatcag
     5461 atttatgtaa tggtcaaaag atctttacca tatgagtttc agcaaaactt tatttatgct
     5521 atgagcttgc atatcagttc ttttttgaaa aaaatcaact taggagaaga acggcatacg
     5581 aatgataaca tccgtgagat ggcttttgat tttccagatg aatatgctgt tgcgaaagaa
     5641 gtgcgtagtt atatcgaaac gtattttcaa gtggagattc cagaaagtga agactattac
     5701 ttgaccgttc tactcgtctc gttacgggca aatcaagcaa gcggcagaat cggtgtagtc
     5761 gttgcagctc acggaaacag cacagcaagc agtatggtcc aagttgtcca acagttgtta
     5821 gatgcagaga acgtacgagc agtagacatg cctttggata tggatccgaa aacagcattg
     5881 gaccgaattg aacgcaatgt acaagaagta gatgaaggaa gcggggtcat actcttagta
     5941 gatatggggt cccttgcgtc gtttaattcg caaattcaaa gagacactgg aattccagta
     6001 cgtactgttg atatggtaac aacttctcta gttttggaaa ctgtacgaaa ggtctcagta
     6061 ttagggacag atctagatat gttgcatgaa tcattgaaaa attttcgagg ttacgcagaa
     6121 atatcttctg tagaatctgt tcaggaagca aaacctattc ataagaaaaa agctgttttg
     6181 gctatctgcg catctggcga aggtacagca caaagaatca aagaattgat cgaacgagcg
     6241 gtaaacaagc gtcaagaaac agaattagaa gttctagctt tgtcgattgt ggaagtgaaa
     6301 gaagaactgc cagttatcca aaaagaatat cagatcatag ctactactgg gattactgat
     6361 ttgaatatcc aagccccatt tattccttta gaacgtttta ttgatcaaaa tatcgaagtc
     6421 attttggatc agttgctgat ggaatcagaa ttggaggaaa ctgaattcat ctccctagat
     6481 gaagagtcag ctaaaaatac gtgtgttgag tttatctctg ataacttcat ctttattaat
     6541 ggtagtaagt taatcgatcc aatgtggcaa ttttctacac aaatctctca gacgacggga
     6601 atcggcgatg aagaatatgg attcaaaatc aacctagtga tgcacacagc aggaatgatc
     6661 gaacgtatta ttcggaatga accattgact gtggaagaaa acgaattgac aaatacaacg
     6721 aatgatcctc tttatagcca attggcagcc tcagttgttc tgttggaaga tcagataaaa
     6781 gtaaaagtgc cgatagaaga aatgtattat ctgctacgtc tagtccataa tcagttagat
     6841 aaaaaagaat acactgtgcc atgaatacac aaaaacgata cacttttata gtgtatcgtt
     6901 tttgtgtatt ggttaagaat cctttaaatt caattagtgt atcgtaaaaa agttggcacg
     6961 ctttttgctt atagtagtgt gtgctaggag gtggttgttc acacagaaat gataggggtt
     7021 tctgtgaagt agtgaggaag aaggagtttt gcagatggat attcgattgg cacgtataga
     7081 tgatcgttta atccacgggc aagtcgcaac agtttggtcg aagcaaacag gtgttgaacg
     7141 tatcattgtg atcagcgatg tagtcgctca agataaattg agaaagtttc tactaaaaga
     7201 agctgcaccg ccggggatca cagcaaatgt aataaccgtg cagaaaatga ttcaaatcta
     7261 tcatagtgga ttattatcac gagcaaaagt gatgctttta ttcaccaatc cccaagatgt
     7321 tgaagcggtt gtccgctgcg gtgtacagtt gaagtcgttg aatatcggcg gtatgagttt
     7381 tactgaaggc aaatcaatga ttaccaattt tgtttcagta aatcaacaag atattgattc
     7441 ctttcgttac ttgaacagtc aagaagtgga gttagagatt cgaaaagtac ctgctgacag
     7501 gaaaatcctt ttgatggaac tactagcgaa aatcaaaaag gtgtagcaag aatggttgtt
     7561 ggaaaaggtc aaataattct gttgtaatgg ttagcaatta ttaatatttt tgaatttgtt
     7621 acaacaaaag taaataggag gtataaatat ggtagggatt atcctagcaa gtcatggaca
     7681 atttgctgaa ggaatcttac aatccggttc aatgatcttt ggcgaacaag aaaacgtcaa
     7741 agccgtgatt ttgaaaccaa gtgaaggtcc agatgacttg agagccaaat tggaagaagc
     7801 ggttgcttct tttgataatc aagacgaagt gttattctta gttgatttat ggggcggaac
     7861 accgttcaat caatcaaaca cattattcga agagcacaaa gataaatggg caatcgtcag
     7921 cggtttgaat ttaccaatgt tgattgaagc ctacgcatca cgcttttcaa tggaatctgc
     7981 tcatgaaatt gcagcacaca tcattgaaac agcaaaagac ggtgtaaaag taaaaccgga
     8041 agaattagaa ccggcagaag caccgaaagc agcagttgaa gacgcacaac ctaaaggagc
     8101 acttccagaa ggaactgtgg tcggagacgg aaaaatcaaa tatgtattag cacgtgttga
     8161 ctcacgtctg ttacatggac aagttgcgac agcttggaca aaagcagtgc aaccaaaccg
     8221 tattatcgtt gtttcagatg cagtatcaaa ggatgatctg cgtaagagac taatcgaaca
     8281 agctgctcct ccaggtgtaa aagcaaatgt tattcctatt agcaaaatga tcgaagtagc
     8341 aaaagaccct cgttttggaa ataccaaagc attgctttta tttgaaaatc ctgaagacgt
     8401 attgacagct gttgaaggtg gcgtagacat caaagaatta aatgttgggt caatggcgca
     8461 ttcggtcggt aaagtcgttg taagtaaagt attgtctatg ggacaagaag acgttgaagc
     8521 gtttgagaag cttgaacaaa aaggtgtgaa attcgatgta cgtaaagtac ctaatgattc
     8581 acgcgataac atggatgata ttttgaaaaa agcaaaagct gaattagcaa aagcgtaata
     8641 aataatatta aaatttatag gaggcttatc atgtctatta tttcaataat tttagtcgta
     8701 ttagttgcct ttctagctgg tatggaaggt attcttgatg aatttcaatt tcatcaacca
     8761 ctagtggcat gtacattgat cggtttagtt accggtaacc tcgaagctgg tatcgtactt
     8821 ggcggtacgc ttcagatgat cgctttaggt tgggcaaata tcggtgccgc tgtagcacca
     8881 gatgctgcat tagcatcagt agcatcagca attattttag ttttaggtgg acaaggtgtt
     8941 aaaggtgttc cttctgcgat cgcgattgcc gttcctttgg ctgttgcagg gttgttctta
     9001 acaatgatcg tacgtacaat cgctgttcca atcgttcatt tgatggatgc ggctgctgaa
     9061 gaaggaaaca tccgtaaggt tgaaatgtgg cacatcattg ctgtatgttt acaaggggtt
     9121 cgtatcgcgg tcccagcagc agcgttgcta tttatcccag cacaaactgt gcaatcattc
     9181 cttgaatcaa tgccagcatg gttaacagat ggtatggcta tcggtggggg aatggtcgta
     9241 gctgtaggtt acgctttggt aatcaacatg atggctacaa aagaagtatg gccattcttc
     9301 gttattggtt tcgttgtagc tgcaatttct caattaacat tgattgctct aggtgcttta
     9361 ggtgttgcat tagctcttat ttacttgaac ctttctaaaa tgggtggttc atcaaataat
     9421 ggaggcggaa gcaactctgg tgacccttta ggcgacattt tgaatgacta ttaatcttga
     9481 aggaggaaaa taaaatggca gaagaaaaaa tcaaattatc aaaaaaagat cgtttagctg
     9541 ttgcgtggcg ctctaccttc attcaaggtt catggaacta cgaacgtatg caaaacggtg
     9601 gctgggcatt ttctatgatt ccagcaatca aaaaattata caaaacaaaa gaagaacgtt
     9661 ctgcagcatt aaaacgtcac ttggaattct ttaatacaca cccatacatt gcttctccaa
     9721 ttttaggggt tacattggct ctggaagaag aacgtgcgaa tggcgcgcct gttgatgatg
     9781 tagcgattca aggggttaaa gtaggtatga tgggtccttt agctggtgtt ggtgacccag
     9841 tgttctggtt tacagtacgt ccaatgctag gtgcactagg tgcttcatta gctatgggag
     9901 gaaatatcct tggaccaatc atcttcttct taggttggaa cattattcgt tgggcattca
     9961 tgtggtatac acaagaattt ggctacaaag ctggttctaa aattactgat gacctttctg
    10021 gcggattgtt acaagacgta acaaaaggtg cttcaatttt agggatgttc gtattagctg
    10081 cccttgtaca aagatgggta tcaatcaaat tcttaccagt tgtatcaact gtaaaattag
    10141 ataaaggtgc ttacatcgaa tgggataaat tgccttcagg tggtgaagga atcaagagtg
    10201 catttgaaca agttaataat ggcttagccc tttctcctga aaaggtaaca acattacaag
    10261 acaacttaga ccaattgatt cctggtttag ctgcattagg attaacattg ttatgtatgt
    10321 ggttgcttaa gaaaaaggtt tctccaatcg ttatcatcct tggtttattc gtagtcggtg
    10381 ttgttggaca cgtcatcgga cttttataat ccataaaaat atagctcggg cgttgtcccg
    10441 agcttttttt atatagtata ctgttcataa acaaatatct ggagggagaa aatggttcaa
    10501 tcaatcaata caaaagtaga cttggtaatt gatgctactt cgtttacggg tctgacagat
    10561 tacgggcaaa tcatgatagg tgataatggt tttgagtttt accatgcgcg agatagacgc
    10621 aaatatatcc aaattccatg ggaagaagtg gattatgtac tagcatcagt catgctaaag
    10681 ggaaaatgga ttcctcgata tgccattcaa acgaaaaaaa atggtacata cacattttct
    10741 tccaaagaac ctaaaaaagt gcttcgtgcg atcagagaat atgtagatga agatagaatg
    10801 gtacaatcat tgagtttctt tgatgtagtg aaacgtggta ttcgtagtat gtttaaacgc
    10861 aagtaaaaaa taaagaaaac ttctaagagg aggttttctt tattttttta ctttgcagaa
    10921 gaaaatatga aatagcaaaa ccaattattt gtccccttta aatcccatat gctaaaattg
    10981 attagcaaag aaggaggtta tattatgagt tggttatgga cattgattgt tggagccgtg
    11041 atcggagcaa ttgcaggtgc gatcaccagt aaaggaaatt cgatgggctg tgtaacgaat
    11101 atcattgctg gactagtcgg ttcaagtatt ggacaagcga ttttcggcga atggggacca
    11161 aaagctgcag gtatggcact tattccatct attttaggcg cagtgatttt ggtagcagtt
    11221 gtctcatttt tctttggaaa acgatcataa gtttttacaa aatagtaaac taaaagga
//
LOCUS       DAYYCY010000082        11238 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000082 DAYYCY010000000
VERSION     DAYYCY010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11238
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.082"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(301..2310)
                     /gene="metG"
                     /locus_tag="ACHAZB_001741"
     CDS             complement(301..2310)
                     /gene="metG"
                     /locus_tag="ACHAZB_001741"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFM8614388.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            2578..2856
                     /locus_tag="ACHAZB_001742"
     CDS             2578..2856
                     /locus_tag="ACHAZB_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8614389.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            2953..3774
                     /locus_tag="ACHAZB_001743"
     CDS             2953..3774
                     /locus_tag="ACHAZB_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFM8614390.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(4059..5069)
                     /locus_tag="ACHAZB_001744"
     CDS             complement(4059..5069)
                     /locus_tag="ACHAZB_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFM8614391.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(5081..6034)
                     /locus_tag="ACHAZB_001745"
     CDS             complement(5081..6034)
                     /locus_tag="ACHAZB_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8614392.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            complement(6024..7094)
                     /locus_tag="ACHAZB_001746"
     CDS             complement(6024..7094)
                     /locus_tag="ACHAZB_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8614393.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            complement(7100..8143)
                     /locus_tag="ACHAZB_001747"
     CDS             complement(7100..8143)
                     /locus_tag="ACHAZB_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8614394.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            complement(8144..9085)
                     /gene="opp3b"
                     /locus_tag="ACHAZB_001748"
     CDS             complement(8144..9085)
                     /gene="opp3b"
                     /locus_tag="ACHAZB_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFM8614395.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            9169..9516
                     /locus_tag="ACHAZB_001749"
     CDS             9169..9516
                     /locus_tag="ACHAZB_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HFM8614396.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            complement(9586..>11238)
                     /locus_tag="ACHAZB_001750"
     CDS             complement(9586..>11238)
                     /locus_tag="ACHAZB_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8614397.1"
                     /translation="KFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFNLVV
                     PQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTYKIK
                     LREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKSELG
                     IKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNGPFT
                     LEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDVILS
                     GELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVKQVL
                     GDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDSLEF
                     DLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGGWAA
                     DYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIKIIS
                     DDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
BASE COUNT         3161 a         2294 c         2054 g         3729 t
ORIGIN      
        1 gcattcaaac tttatgaatg taaggacaat aaaaaagagt accagatgaa tcaattaaaa
       61 tctgcccgac cctttatcat tatgcttttt ttctgttttt acgattccta ttagtattat
      121 tctaaattaa caaaaatcag cgaagctcgt acttcttttt ctaggattta ttttggatag
      181 ttctctgttt ctgtattata tgattggctt caagtaataa gaaggaattt acgaaaattg
      241 cttctcaaat ttcggaaaaa gggggtatga cacaactagt gtcatacccc ctttgaattt
      301 ctatgcgatt tctgagccat ttggcatgga ttttggtgct tcgattactt gtaaagagcc
      361 atctggtgct tctgcagaaa ggatcattcc ttgactgatt tgtccacgca tctttcgtgg
      421 tttcagattt gctacaatga cgactttttt cccgatcaat tcgcttggat caggataaaa
      481 ttcagcgatt cctgacaaga tttgacggtc ttggctgtct ccagcatcta gtcggaattg
      541 cagcaactta tctgcccctt ctactttttg acagttgatg acttctgcta cttttagttc
      601 tactttttca aatacatcaa atttgatttg tttttctttc gttgaaacaa gttctgtttc
      661 ttctggatcc catttgactg tatcttcgtt tgtttgagta ccttcagaca ttttttcttg
      721 aataaaagca acttcttctt ccatatctaa acgtgggaag attggtgtac cttttgcaac
      781 tacttttgtt cctgaaggaa attcgccaaa gtgtaatcct tcaagattca ttgtttcaga
      841 gtccaatcct agttgattga agatcttctc tggcgtttct gtcatgattg gctgaagtaa
      901 tatcgctgcg atacgcaaag attcagctaa atggatcatg acactattca attcagcttt
      961 cttttcttcg tcttttgcta atacccatgg ttctgtttca tcaatgtatt tgtttgcacg
     1021 agaaatcagc acccagattt cagccaatgc tgtattgaat tccatttttt ccattgcatc
     1081 atggtaacgt ccgacaacgt tagcagccgt cgtagacagt tcactatcaa atggagtgac
     1141 aagtgaggca tagtcaggaa cgagtccatc acaatatttg ttgatcatag caatcgttcg
     1201 attcaataag ttccctagat catttgccag atcataattg acacgggaaa caaaatcttc
     1261 tggcgtaaag acaccatctg acccaaacgg aacagcacga agcaaataat aacggagtgc
     1321 atctaaacca taacgttcca caagcatttc aggataaacg acatttcctt ttgatttaga
     1381 catttttccg tctttcatca atagccagcc atgcccaaat acttttttag gtagaggcaa
     1441 atcaagggcc atcagcatga ttggccaata gatcgtatga aagcgaacga tttctttacc
     1501 gaccatctga acatctgcag gccagtattt ttcgaataga ctttcatctt cggagccata
     1561 tcctagtgca gtaatataat tggataatgc atcgatccat acatacacca catgtttcgg
     1621 atcactcttg actggtactc cccaagtaaa agttgttcgt gaaactgcaa gatcctctaa
     1681 tcctggtttg ataaagttgt tgatcatttc gtttttacga gattctggct ggatgaattc
     1741 gggatgttct tcatagtatt ccaatagacg gtctgcgtat ttgctcatgc ggaagaagta
     1801 tgattcttct tttaccaatt ctacttcgtg tccacttggc gctttaccac cgatcacgtt
     1861 accttcgtca tctttgtaca cttctgcaag ttgtgtttct gtaaagaatt cttcgtcaga
     1921 aacagagtac cagccttcat attcacctaa gtaaatatct ccctgttcta ataaacgatc
     1981 aaatattttt tgaacagctg ctttatggta atcatctgtc gtgcgaatga atttatcata
     2041 actgatatca agcgttttcc ataatttctt gacatcagcc gccattttat ctacatattc
     2101 ttgcggtttt acacctagtt cttctgcttt tgtttcgatt ttttgaccat gttcatccac
     2161 tccagtgaga taaaacacat caaagcccat caaccgttta taacgagcaa ttgcgtcaca
     2221 tgcaatcgta gtatacgagt tgccgatatg aagtttgccg cttggataat agattggcgt
     2281 agtgatataa aaagtttcct tttctgccac ggggctcttc ctcctttatc gcatgatgcg
     2341 aattcgtttc cttgctagta taccataaaa aaagcatgct gttagctttt tttcaaaatg
     2401 aaaaagactg gataaacgac ttctatctac gttcaatcta tcctttaaaa ggatgaatag
     2461 gttgaataaa tgcgggtttt tgttttgttt catatctact aaaaaacggc tttttatttc
     2521 tcccctcaca cgtggtataa taaagactat ctatcttagt aagggactgg aacaatcatg
     2581 cagaagaaac atcttttttt tacacttagt attgcttttc tttcattagc acatttaata
     2641 ttcagctatt tttatattcg tatgtatggt tattttaacc tccatgggca tttgaacagt
     2701 tttatgacag cagcttggat tttgcgtttt atcattgacg tatatatcgt tatttgtggc
     2761 ttctttgcca ttcgtgaaga gcggtataaa gtattgccgt tttatctgct tttcttttta
     2821 ttcaatctca tacttccatt tatttttcat atttaaatga taacctagat ttttcaagta
     2881 aatccttggt tttttttcag agtctgctac aataaggtaa aaagtacttt tactgtcaat
     2941 ggaggtggtc agatgaaccg ttttgcttct aaagaagaag aaaaattata ttatgaacat
     3001 aaagcaagtg aagaagaatt tcttaactgg tatcaaaaac aggaacgtcc agaatatgac
     3061 aagccctctt tgactgtcga tatcgtactg atgtgctata acaaagaaga agatcagctg
     3121 aaagttttgt tgatccaaag aaaagggcat ccttatcgaa attcatgggc attgcctggt
     3181 ggctttgtcc aaaaggatga gtcaactgga gaaagcgtct taagagaaac aaaggaagaa
     3241 actggtgtgg ttatttcaaa agaaaatatt gagcaactgc atacattcag tacgcctaat
     3301 cgagatccgc gaggctgggt cgtcacagtc agctacttag cctttattgg agaagaaccg
     3361 ctaatcgccg gagatgatgc caaggaagta cgctggttca cactagaacg tcatagtaac
     3421 aagatccatt tatctagcgg agaagtggct atctcgcttg atttagtcac tggagagtct
     3481 tctggcaaag atactttggc ttttgaccat agtcaaatca tcttgaaagc attcaaccgt
     3541 gtagtcaata aaatggaaca cgagccgcaa gttctacaag ttttaggtaa agatttcacg
     3601 attacggaag ctcgaaaagt atttgctaaa ttcttaggga tcgattataa aacgatcgat
     3661 cattccaatt ttaagaaagc tatgctccaa tatttcgatg agatcggtga acgtcctgta
     3721 ggaatcggcc gtccttcaaa aatctatcag ctcaagccgg ggcaccatga ataaggttgt
     3781 gagcaaaaga ttcagaccta atcattcaag aaaaaacgca aaaacaactc tttgtgagtt
     3841 acgttttttg tttcagaatt ttgatgtatt cgtaaacttt ctgaataaaa aattaacaat
     3901 agcagaaaaa gctcgtacga actttttctg ctattgtttt attggtttgt cataattagt
     3961 gagcttttct tcaaaagaaa aattcctaga aacaaaagtc attaagactt tttattaaca
     4021 aggctaaccc taacgctcct atcagtttca ttcatttttt atcccttgat aaccgaatgt
     4081 ctgcccattt tttctactac ttggctcttt cttacggaat tgctttcttc tgctccgtcg
     4141 gtattgatca aaagaaattt tttattatac cattgggact tataacgcaa ttttgttatt
     4201 aatttatgcc agtcgtgaat agaaaaatca gcatgtgcct cttcttttgg aaaagattct
     4261 acaaataagt agtcatattg tttgaccaat ttcccagata attgttcaag ataatcttct
     4321 cgctggttct tttgtttcag ataaagatcc attacttttc tcttttgttt ctgataattg
     4381 cttgcctgat ctaacctgat ctttctatgg tgagcggagc ggactttcac attcaatttc
     4441 cgctgtgcat gttgcagctt ttcttctgtc tggtgaaatt ttggtaaagt tacttctaga
     4501 ggtcgagatg tttggatcaa tgtcttcgga tgataggcga tcccgatcca tttagctaaa
     4561 gattcttttt ttactggcga aacatcacaa agtagtgcga cataaaattc ttcattatat
     4621 cgagcagaaa tcgtagcgga gcgaatcgat ccttcaactg gccgatgctg atttatcttg
     4681 attagttctt tttgttttgg taactttaag tagccatttt ccagatagat cgtacgggtt
     4741 tggttgttgg tcgtataaga ttgccacata ctttttttcg ttttaagctt tgggaagctg
     4801 gcacgtccct tgaaataatt gcgaaaagcc cgatccaaat taagctgtgc atttgctaaa
     4861 gcaagactgt ctacttcttt taagaatggg tattcttttt ttaatgtagc gggtgtgagc
     4921 gaaaaattgg ctactcctgt ttgttgccaa cttttctgcc gctttttcag caaatggtta
     4981 taggtaaatc gagcacatcc aaaagtacga atcaattgct gtttttgttc ttctgtcgga
     5041 tacatacgga atttaaaagc ttttagcatc tatgtccctc ctagttagca accagaagag
     5101 attccttttg tcgttttagt ttttttgcgt aatatgggac ttcctgctca gttgcataga
     5161 caaaatgttc tggtgcgatt tcacgaagtt gacgtacttg tccgtcatct ggttcaggtt
     5221 gatatttgat tcttctgcgt tttctctcat ggtctggatc tggtagagga atagcagata
     5281 acaaactttc tgtataagga tgaacaccgt gattatagat ttcatcactt gttcccatct
     5341 ctaataacaa gccgttatgc attacaccga ttcggtcact aatatgttta accatcgaca
     5401 aatcatgtgc aataaataga taggtcaatt gatgttcttt ttggagatct tgcaacaaat
     5461 tgaccacttg ggcttggatc gacacatcta gtgcagaaat tggttcatca caaatgataa
     5521 aacgcggttt tactgccaat gctcgtgcaa taccaatacg ctggcgttgc ccacctgaaa
     5581 attcatgagg ataacgagta ccgtggcttg gattcaatcc aacagttctc aatagatcat
     5641 cgactttttt tgcacgctct tcagatgatt tagctagtcc attaacatca attccttcgg
     5701 caatgatatc tctgactttc atacgaggat tcaacgaagc gtatggatct tgaaagatca
     5761 tctgaacatc atgccggaat ttcgtcaaac cttttttatc tttgatcttc gttatatctt
     5821 gaccgtcgaa taatatctca ccatcagtag gttcattcaa gcgaatgatc gtacggccag
     5881 tagtggattt tccgcttcca gattctccaa ctaaaccgaa tgtttctcct tcataaatgt
     5941 gaaagctgat gtcatttaca gcatgtactt cattcttttt tcctacattg aaatactgtt
     6001 tcaatccttt aacttcaagt agtttatttg gcatcagcaa ttcctccttg accgtaagta
     6061 tgtttttgtt tctctttaaa gatagcccag cgacgttgga tttcttctgg cggtgtcact
     6121 ttgggtgcct gcggagcaag tagccaagtt gctgccttgt gtgtttttga tagttcaaaa
     6181 aatggtggtt cttgttctgc atcgattttc atcgcgaatt catttcttgg atagaatgcg
     6241 tctccttttg gcgggttcaa taaatccggt ggagaaccag gaatagcata caatcgatcg
     6301 ttcgcactat ctaatgtcgg cattgaacca agcagtcccc aagtataggg atgttgcggg
     6361 ttgtagaaaa tttcttccga tgttccaact tcaacgattc ttcctccata catgacagcg
     6421 acacgatcag cgacattcgc tactacacca aggtcatgag tgataaagat aattgatgta
     6481 tcgatttttg tctgaatttc tttcagcagc tccaaaatct gtgcttgaat agtcacatca
     6541 agcgctgttg taggttcatc agcaatcagg atttccggat aacataccag tgcaatcgcg
     6601 atcacgatcc gttgacgctg acctcctgag aactgatgcg gataactgtt gatacgtttc
     6661 tcagcttcgg gtattccaac taatttaagc aattctaatg cagctttttg tccctctttt
     6721 tttgaaactt tattatgctt tctaagggac tcagcgactt gcttcccaat gggcatcgtc
     6781 ggatctaaag aggtcattgg atcttggaaa atcatcgcta tttctttacc acggattttt
     6841 tgcatctctc tttctgattt tccaacgata tcgttacctt taaagaggat ttgaccagca
     6901 tctatattgg cgttgcttga cagcaagcgc atgatgcttc tggtcgtcac tgattttcca
     6961 cttccagatt ccccaacgat cgccaatgtt tctcctttat tcaaatgaaa attgacaccg
     7021 cgaatcgctc gtacctttcc agcatacgta tcaaaagaga tttctaagtc tttcacttct
     7081 aaaattttcg tcatcgtctt cactctttca ttttaggatc aaaggcatca cgcaatccat
     7141 cagcaagtaa gttaaagcca atcatgataa ccgataaagt tactgccgga atccataata
     7201 gataaggcag atacaggaat gtcttgtacc cttcgctcaa catcgttcct aatgaagctg
     7261 acggaggcgt aagacctaaa ccgataaagc ttaaaaaggc ttcgaagaaa atagcagatg
     7321 gaatactaaa catcatttga ataataatga cactagagat attcggcaaa atatgtttga
     7381 atgcaatttt cattcttgat tcacctaatg tcaatccagc taaaacatat tcttgatctt
     7441 ttaatttcat tgtttgtgca cgaacgatac gtgccattgg tatccagttt gtgatggcca
     7501 tagcagccac gattgaaaaa attcctggtt caaaaacgac caacatcaaa atcatgacaa
     7561 cgaggtttgg aattcctgat aagatttcca aaaaacgttg catcacgtta tcgacacgtc
     7621 cgcctaacaa accagaaacc aacccgtaag ctacaccaat cgttacatct agtaatgcag
     7681 caataaatgc gatcagcaac gagattctca ttcccataaa taagcggctt aatacatcac
     7741 gtcccaaacc gtctgttcct aaaaagaaat ttacgtttga agggacgttt gcttgagcat
     7801 atttatcaac aagttcacca ccaaccattg cttttccatt caatccatcg atattgattc
     7861 ccggaatacg aggaggcaaa ttgatataag ccacgttttg ggcagttgga tcatgaggag
     7921 aaacaaagat tgtgatcaca gagatcaaaa tgatgaccaa tagtaaaacc attgaaataa
     7981 cggcagcttt gtttttcttt aaacggcgcc atgaatcttg gataaaattc aaagaaggtg
     8041 cagtaatctg ttcacgttct tcttcggtat ttttttgtaa tggaagaaat tcatccgcag
     8101 ggatatctgc aactgtttca taacgctttg gtatcatttc catttatcct cggctccctt
     8161 ctgaaacacg aattcttgga tcaactagac cgtacaatag atccacgatc aaaataacaa
     8221 aaatcagcat aaatgagtaa aggatcgtta cagccatgat cgtaggataa tcatttgttg
     8281 tgattgactt aacgaattgt tccccgatac caggaatagc aaagatattt tctaccacca
     8341 gtgatccggt catcaatgct acagctaacg gtcctaataa agtcatcaat ggaatcaaac
     8401 tatttcgaag accatgttta aaggcgattt cccaacggct caatcctttt gcttttgcta
     8461 attccacata atcactatgc agtacttcca ccatttccgt acggataaac cgagcagagt
     8521 cagcaagcgg cgacatggcc aacgcaattg ttggtaagat tgtgtacaca aagccatccc
     8581 attttgcgat tggcaaaact tgtaatttga ttgcaaagat atattgtaat aaaacagcaa
     8641 atacaaagtt tggaatggaa cgacctaata tcgctacgag tgtagaagat gtatctgccc
     8701 atgtattttg cttcatggca gagatcgttc ctaaaatcgt ccctaggact gttccaaaga
     8761 taattgcctg taaccctaac tgcaaggatg gtccgattct tccagctaac aaatgtgcga
     8821 ctggctggtt tttaaattgg aaggaaattc caaaatctcc ttgaagcaaa ttagaaagat
     8881 aaatcccata ttgtacgata actggtttat ccaaaccgac ttgtttattt agcatctcga
     8941 ttgtttcagg actcaaacgt tcctgattcg tatatggcgt gcccggcagc aattgcatca
     9001 agaaaaaggt gattgttgca attaaccaaa gcgtgatgat catgaaaaag acacgtttta
     9061 atacatattt gatataacta ttcactaatg tttcccccta gcttttgact tctgacagat
     9121 gtttcgctac attattagat aatgaaccaa aaataaagga gcaatcaaat gaaaaaaaaa
     9181 tctatcccct acgcagtagc ttttctactg attctggtta ttttaataaa aaatctcatc
     9241 aaccattcct ttacactcat tcaattatct aatgatctat tcttatggtc attgccgttt
     9301 ctgattatcg gcggtttttt atgggtcttt tcttcgggtt tcttcgatca ttttcagcga
     9361 tcggtccatc ttgcacgaac aaggaaccga aagaaaaaac cagaattttc ctcgctctcg
     9421 tctgccagct acggtatgta cagcttttgg ctgatcattg ccggaatact aattgctctt
     9481 tctgctattt tcatgctttt ttctttgtta ggataagtga atatgagaac gaggttgcga
     9541 catttgccgc agcctcgttt tttctcatgc tcacggtatt aacgattatt cttcaacgta
     9601 tgtccatttg taatcccatg aagcgcctgc cgcatggtga acgatacctt tgattttttc
     9661 attcactagg tgaccttctg ctttttggta caccggaacg acacccatat catcagagat
     9721 gatcttgatt gctttttgga aatcatccca acgggcttgt tcatcgccag cgtctgttga
     9781 tgaagctgct ttaactgctt cgtcatattc tgcattgctc cagcgaccac ggttgtatga
     9841 attacctgtg acaaacaaat cagtaaagct gcttgggtct gcatagtcag cagcccatcc
     9901 gcctagtact gcgtcaaaat cacccgaagt tgagcggtca agacgtacag agaatggaac
     9961 aggtgttggt ttgactgtta cgccatccaa gttttcttgg attgagtttt gaacatattc
    10021 gattactttt ttcgatgaat catcatcaga agccatcaaa tcaaattcta aagaatcaac
    10081 gcccaattct tttttcgctt tttcccaata ttcttttgct ttatctttgt cataagaaac
    10141 taattttcca gcttcttttg caaagtcttc gttcgttgtt ggacttttta ccatatctga
    10201 tgggatcaat cctgtagaga caactgatcc gtcacctagt acttgtttta ctaaagcttc
    10261 acgatcgata gaataagaaa tagcttttct taggttcaca ttgttgaatg gtgaattctt
    10321 ttcacgttga ttgaattcga tatagcttgt ccgcgcttct ttaacagaag tataagctgg
    10381 atcgtttgcg ttttgctgtg ctaactcacc agacaagatc acgtcatctg cttggccatc
    10441 tttgaataag ttcaatgcag tagaagattc ttttactaca ctgactttga tttcagaaag
    10501 ttttactgta tctttgtccc agtaatgatc atttttctta taagtccatt ctgtatctgt
    10561 tcctggtcca tcaaaatctt ctaatgtgaa tggtccatta taaacagctt tatcacttgc
    10621 agatgcatag gcatccccat tttcttccac tacggattgt ttttgtggga aaaatgatgg
    10681 gaaagcaagc agatattgga aatatggtgt ttgtttcgtc aatttgattt ctaattcata
    10741 gtcaccaact gctttgatcc ctagttctga tttatctttc tttccagctg taatgtcttc
    10801 tgcattttca actggcgcat acaggtaagc atattcagaa gctgtagctg ggtctactgt
    10861 tcgttgccag ccataaacgt aatccgctgc tgtaacagga tcaccatttg accattttgc
    10921 gtcttctcgt aatttgattt tgtaagttaa tccgtcttca ctgacttctg ccaactcgct
    10981 agctcctgcc ggctgtggtt tgctgtcttc atcaagacgg taaattcctt cataaacgtt
    11041 attcaatgct gtaaaactga ttgtatctgt tgcaactgat aagtcagctg taggcatttc
    11101 ttgaggcacg actaagttaa atacgcctcc tccatctgct gaaccgcttt ttgctgaact
    11161 actgcttgtg gctgaactgc cgccataaca tccagcaagt acaaggccgc ataccgcaac
    11221 caaaccaaat gtaaattt
//
LOCUS       DAYYCY010000083        11207 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000083 DAYYCY010000000
VERSION     DAYYCY010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11207
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.083"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..305
                     /locus_tag="ACHAZB_001751"
     CDS             6..305
                     /locus_tag="ACHAZB_001751"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8614398.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="ACHAZB_001752"
     CDS             378..1739
                     /locus_tag="ACHAZB_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFM8614399.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="ACHAZB_001753"
     CDS             1761..2021
                     /locus_tag="ACHAZB_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8614400.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="ACHAZB_001754"
     CDS             2035..2787
                     /locus_tag="ACHAZB_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HFM8614401.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="ACHAZB_001755"
     CDS             complement(2881..3486)
                     /locus_tag="ACHAZB_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8614402.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="ACHAZB_001756"
     CDS             complement(3539..4513)
                     /locus_tag="ACHAZB_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HFM8614403.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="ACHAZB_001757"
     CDS             complement(4891..5610)
                     /locus_tag="ACHAZB_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8614404.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACHAZB_001758"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACHAZB_001758"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0019191 - cellobiose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFM8614405.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWIVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="ACHAZB_001759"
     CDS             7161..8558
                     /locus_tag="ACHAZB_001759"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8614406.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="ACHAZB_001760"
     CDS             8639..9079
                     /locus_tag="ACHAZB_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFM8614407.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="ACHAZB_001761"
     CDS             9227..9553
                     /locus_tag="ACHAZB_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM8614408.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="ACHAZB_001762"
     CDS             9554..10303
                     /locus_tag="ACHAZB_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HFM8614409.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="ACHAZB_001763"
     CDS             complement(10383..10838)
                     /locus_tag="ACHAZB_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFM8614410.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
BASE COUNT         3554 a         1959 c         2300 g         3394 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aatccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagtt
//
LOCUS       DAYYCY010000084        10922 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000084 DAYYCY010000000
VERSION     DAYYCY010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10922
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.084"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..281)
                     /locus_tag="ACHAZB_001764"
     CDS             complement(<1..281)
                     /locus_tag="ACHAZB_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8614411.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEA"
     gene            453..1541
                     /locus_tag="ACHAZB_001765"
     CDS             453..1541
                     /locus_tag="ACHAZB_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM8614412.1"
                     /translation="MFWSLELLIIAALIMISSEINFIFEPIGTFFSTLFAPVLIAGFL
                     YYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQISSLASNMPDF
                     IKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSLGSIVGTIASA
                     TVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTLANYISGQAIE
                     CLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLVTVFNDPVKAL
                     LCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLGIFLGVPFYAI
                     CKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            1669..3045
                     /locus_tag="ACHAZB_001766"
     CDS             1669..3045
                     /locus_tag="ACHAZB_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFM8614413.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            3446..5026
                     /locus_tag="ACHAZB_001767"
     CDS             3446..5026
                     /locus_tag="ACHAZB_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFM8614414.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            5224..5447
                     /locus_tag="ACHAZB_001768"
                     /pseudo
     CDS             5224..5447
                     /locus_tag="ACHAZB_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
     gene            complement(5652..5972)
                     /locus_tag="ACHAZB_001769"
     CDS             complement(5652..5972)
                     /locus_tag="ACHAZB_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFM8614415.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(6120..8354)
                     /locus_tag="ACHAZB_001770"
     CDS             complement(6120..8354)
                     /locus_tag="ACHAZB_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8614416.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            8833..9099
                     /locus_tag="ACHAZB_001771"
     CDS             8833..9099
                     /locus_tag="ACHAZB_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HFM8614417.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            9099..10826
                     /gene="ptsP"
                     /locus_tag="ACHAZB_001772"
     CDS             9099..10826
                     /gene="ptsP"
                     /locus_tag="ACHAZB_001772"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFM8614418.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
BASE COUNT         3479 a         1911 c         2215 g         3317 t
ORIGIN      
        1 gcttcttcaa tcaatatttt tcctaaccct tggtgtctgt attcagaaag aatcgccatc
       61 cgctgtaatt tcatttttcc gttttctaaa ggcaaaagac gtacagttcc ttgcggttct
      121 tttttatctg agtacaggac gaagtggata caatgggctt catctttgtc tatttctcga
      181 gacaaaggca caccctgttc gactacaaaa acttgatgcc tgatccgtac agcatctaag
      241 taaatattgc tcattgtatc ttttgtttgt acaactttca tggtctagcc accttccttt
      301 cgtatagtat actggaaaat aaaacaaata aaaagtatgg caagttttca aaaataaaaa
      361 aagaataaaa gaatttttaa cgaatataat atatactagt aagaaagaaa ggaggaaatc
      421 tattgtttga aaaacttaga cagtctaaac taatgttttg gtcgcttgaa ttattgatca
      481 tagctgcatt gatcatgatt tcatcagaaa taaactttat tttcgaaccg atcgggacat
      541 ttttttccac gttatttgca cctgtactga ttgcaggctt tctttactat ttattgaatc
      601 ctattgtcaa gctgttgatg aaagcaaaaa tcaaaagaat atgggctgtc gcaattgtgc
      661 tgctcctttt agttgcagca attatttgga ttttacttag tgtcatccca agtctagtac
      721 agcaaatttc ctcattggca tctaatatgc ctgatttcat caaacaggta gaaagctggt
      781 taaaagaggt agccgaattg cctttgttca aggaagtaga tatcaataaa tattttgagc
      841 aattagacat ttcttacggg acaatcattc agcaattcct tagtggagta tccaatagtt
      901 taggttcgat cgttggaacg atcgcttcgg caaccgtagt aatcattaca gcaccattca
      961 tcttgttcta tatgttgaaa gacggagaaa aactagtgcc aaacatccaa cagtttttcc
     1021 cagaaaaaag aagaaaccaa attgtggagt tattggggca actgaataaa acattggcta
     1081 attacattag cggacaagca atcgaatgtt tattcgttgg taccttcaca tttttaggat
     1141 atatggtaat tggtgtggat tatgctttct tatttggggt cattgcggga ttgacaaacc
     1201 taattccata tttaggacct tatctaggac tagcacctgc atttctagta acagtcttca
     1261 atgatccggt aaaagcatta ctttgttgtg tggttgtgct aatcgtccaa cagttggatg
     1321 ggaatatcat ttatcctaat gtaattggga aatcactgaa aatccatcca ttaacgatca
     1381 ttatcgttct tctagtagct ggaaatattg ccggattgct tggtatcttc ttaggtgttc
     1441 ctttttacgc aatctgcaag acgatcattt cctatataat caaaatcgtc aaagaagaca
     1501 agcaaaatga aaacaagaag aaaattgcta cgacctcgta ggtagtttta gtacacttgt
     1561 ttttattgca aagaaaattg ctttgtgata ccattttaac gtggctatag tgccacgttt
     1621 ttttctatcg taaactaaaa attaatttta taaggagaga atgcgattat gaatgctgac
     1681 cctgagagtc agtcgctgtt agcgcagatt ttattattga tcgttttgac attgatcaat
     1741 gcttttttag cagcttccga aattgctgtt gtttcgatca ataagaatcg aatcgagcaa
     1801 aaagctgatg aaggagatgt gaaatctcaa aagcttttaa aaatattgca gaatccaaat
     1861 aatttcctat cgacaatcca agtaggaatc actttggtca acattttgtc tggtgcttca
     1921 ctagctaata ctttatcgga acggttagct ccggtattag gtggcggcac agcggcaaaa
     1981 aatattgcaa atatcattgt gctggctatt ttgacctatg tatcgattgt ttttggagaa
     2041 ttgtacccta aacgtattgc tttaaataaa tcagaagaag tcgcaaagtt cacttcaggg
     2101 ctgatacgct tgattggtgt agtagctaag cccttcgtct ggctgctttc tgcttccaca
     2161 agtttattgg cgcgtatcac gccgatgact tttgatgatg aagattcaaa aatgaccaga
     2221 gatgaaatgc gttatatgct tgaaaacgaa ggcgtattga ataatgaaga attagaaatg
     2281 ctccaagggg ttttttctct tgatacgaaa gttgcccgag aagtcatggt cccacgaacg
     2341 gatgcattta tgattgatat caacgacact gtggaagaaa atgtgaatga agttttatcc
     2401 gaaaactatt caagaatccc tgtttataac gaagacaaag ataaagtagt cggaatcctt
     2461 catacaaaga atttactaaa agctgcccat aaattcggct ttgataattt agatatcaag
     2521 aaaatcatgc aggagccttt attcgttcct gaaacggtct ttattgatga tctgttatat
     2581 gaaatgaaaa agacgcagaa tcagatggct attttattag atgaatatgg cggcgtcgtt
     2641 gggttagcaa cactagaaga ccttttagaa gaaatcgtcg gagaaatcga tgatgaatca
     2701 gatgaagttg agaatcttta tgaaaaaatc gatgaacacg aatatatcat ccaaggacgt
     2761 atgctgattg atgagttcaa tgaagctttt gacagtgatc tgcatatgag tgatgtagat
     2821 acgatggctg gttatttgat tacagcatta ggcatgattc cggatgaagg cgaaaagtta
     2881 tcgtttgatg tcgataatat tacgctggtt tctgaagaaa tggaaggttc tcgaatcctt
     2941 aaaatccgtg tcatttttca tgatccggaa gaaacagaag cggaacctga agaagaacga
     3001 agatacttca gaaaagaatt cgaagatgac gagccaagaa gataattgga aacttggtta
     3061 tgaaagacgt gttgcttgaa taaacaagca acacgtcttt tcttttagtc agctgacaaa
     3121 aaaatatttg actcttatgg tttggtggct ttttcattcg ctgaatccat gctatactga
     3181 ttcagttgta gtagttgaca aaggttgtga aaaagctatc ctgcattaag taataagagc
     3241 agccaagcaa aaacagcttt tcgtattttt ggcttgggtg ggcttattgc tgataggctg
     3301 tctctagaac gccgtttact cggaaaaaga gactgctaca cggcttttgt cacagcccta
     3361 tatgaaattg tctttcttta agagatcgga caatctgtgc ctagagaaaa tataaataat
     3421 tgaatggaag aaatggagtt aggttatgaa taatccaaaa ttaaagcaac aagtggacaa
     3481 tcgcagaact tttgcgatca tttcccatcc ggatgctggg aaaacaacga ttacagaaca
     3541 attactattg tttggtgggg ctatccgtca agccgggacc gtaaaaggca aaaaaacagg
     3601 taattttgcc aaatcagact ggatggaaat cgagaaacaa cgtgggattt ctgttactag
     3661 ttctgttatg cagtttgatt atcaagggaa acgagtaaat atcttggata caccaggaca
     3721 tgaagatttt tctgaagata cttatcgtac tttgatggcc gtggatagtg cggtcatggt
     3781 aatcgatagt gcaaaaggga tcgaagcaca gacaaagaaa ttattccaag ttgtaaaaaa
     3841 acgtggcatc ccgatattta cttttatcaa caaactagat cgtgatggtc gtgaaccatt
     3901 ggaattgtta gaagaattag aagagctatt agatatcgaa tcttatccga tgaattggcc
     3961 aatcggtatg ggtaaagggc tagaaggtct ttatgatatc taccataatc gtgtagagtt
     4021 ttatcgtccg gaaaattatc aggatgaacg attagtagaa ttggatgaag atggactgct
     4081 tcccgaaaat catcctctaa ctgaaaattc cttatatgaa caagtcttag aagaagtaga
     4141 actaatcaaa gaggctggag atgcatttaa tcaagaaaaa attgctcgtg gcgatcagac
     4201 accagtattt tttggttctg cattgactaa cttcggtgta cagacttttt tagagacttt
     4261 cgttgaattg gcaccagctc cttatggaca caaaacacaa gatgatcaga tggtaagtcc
     4321 ttatgaggaa gaattttcag gtttcgtatt caagatccaa gcaaatatga atccagccca
     4381 tcgtgaccga atcgcttttg tccgaatctg ttctggaaca ttcgaacgag gaatggatgt
     4441 ctggttggaa cgtacaaata aaaaactgaa attaagcaat gtcacacaat ttatggcaga
     4501 ttcgagagaa aacgtcgaaa aagcagtagc aggcgatatc atcggtgtat atgatacagg
     4561 caattatcag attggggata cattgttcga aggaaaacta aaagttgctt atgaagaact
     4621 cccttcattt actccagagc tgtttatgaa agtgactgca aaaaatgtca tgaaacaaaa
     4681 atccttccac aaaggaatct atcaattagt tcaagaagga gcgattcagc tttataaaac
     4741 atatctaacg gaagaatata tcattggagc tgttggtcag ctacaattcg aagtattcca
     4801 atatcgtatg ttaaatgagt ataacgccga agtaatcatg tctccaatgg gacacaaaat
     4861 cgctcgctgg atcgatccag aagatttgga tgagaagatg agttcaagtc gcaatatctt
     4921 ggctcgtgac cgtttcgacc aaccgttgtt cttattcgag aaccaatttg cagaacgctg
     4981 gtttgcggat aaatatccca atgtaaaatt aaaaagtttg atgtaaagaa gtcttgacac
     5041 aagctgccct gcatcaagta ataagaatag ccaaacaaaa acagcctctc atattttgtt
     5101 tagagccgtg acggattttc cgtgacggct ctttttacta tattaaaatc atagaaatgt
     5161 ctaatcaaaa aaatataaaa attattttgc gtacatatga ttaaagaaag gagagtgtga
     5221 cacatggaaa cagaatggca aagacgtgaa agaaaaaaac agaaaaagaa gagatgttac
     5281 gattatgtcg attattagat gtaaaagact gggacaaaaa cgagcaaatt gcacaaaaag
     5341 tacgatcaat catccataaa gaggaaaaag ttaaaaataa agacacgcgt agaactgaaa
     5401 atgaaaagag aaaagtacaa aagaaaagag aaaaatcaaa aagctagaaa gaaaaagagg
     5461 ctgagacaga agcgtttaac tccaagaaat aagctggaat tcacaaaaat ttgaaaagca
     5521 attttcgtga atttcagctt atttccgaag gagttgcttc tgctttcgcc gtttatacgt
     5581 gtttagagcg tgagacaaaa gtgttcttta cttttgtccc acgctcttta attattatgt
     5641 tcaaaatttg tttacacggc taatgtagcg cctaattcat gttttggaat cgagatttca
     5701 acaaggcctg ccaattcgat tactttgatt tcatcctcgt ggtaaacggc gtctttgatt
     5761 ttatctaaga aataattttt agcatcttcg atttctttcg gctggatttt acgtttgtca
     5821 ttgatcagca gaatttcatt atgttcaggg gcttcagtgt agacaatagt ggtatttccg
     5881 gctgtgtaga ccttgactag atgggcatca gtgttttcaa gctggtttaa tacaagacgg
     5941 gaatggctgt ttgttacatt taccaatttc atggcgctca cctcttttat gtatttttaa
     6001 ttgcttgttt ctcttattat aatttctttt ttataaaatg aacagtattt tgacttttct
     6061 gaaaaaagaa tgagacggca tttctcaaag aaataccgtc tcacgctata aaactggatt
     6121 tattcagccg tttcttcggc tgattcttta gccaaacgtt ctggtttaga agtaacgatg
     6181 atttttccat ctttatccag ttttgctttc aactcatgga tagaagggtg atcaagataa
     6241 aattcagcga taccatcttc gatttgttct tggatcgtgc gtcgaagagg acgagcaccc
     6301 atagatggat catagcctaa gtcaactaat ttttctttga cattcgtagg aacttcgatg
     6361 tgaagttgct gtgctgccag catttggttg acatcgtcta gcattagtgt gacgattgtc
     6421 atcaagtttt cttttgataa tgcactgaat tcgatgatcc cgtcaaaacg gttcagaaat
     6481 tctggtgtaa agtagttgtt taattggttc aatacagaac gagtaacacc ttcgcgtgcc
     6541 gcaccaaaac caacgtttgc ttctacttta ccagttccgg cattacttgt cataatgatg
     6601 atcgtatctt taaagctgac cgttcttcct tgtgcatctg tcaatcgacc atcatctaag
     6661 atttgcaaga acatatgcaa aacatctgga tgagcttttt ctacttcatc taacaagaca
     6721 agactgtaag gatttctccg tactttttct gttaattgac cagcttcttc gtatccgaca
     6781 taacctggag gagaaccaat caatttagag acactatgtt tttccatata ttcagacata
     6841 tcgaaacgaa tcattgaatc ttgtgaaccg aatagttcgt aggcaagttg tttcgcaagc
     6901 tctgttttac ctacaccagt tggtccaaca aataagaagg agccgattgg acgattctgt
     6961 ttgtttaatc caacacggtt tcggcgaatc gctttcgcta ctcgatctac agcgttatct
     7021 tgtccgatga catgagcttt caagtcatct gctaagtttt tcagttgtgt ctgttctttt
     7081 tcttttaatt cacccactgg aatacctgtc cgttgttcaa cgattttttc catatctttt
     7141 tcggtaatca caggtgtttc ctcttcagtg agctgacgtt ctttcatttt ttgaagttta
     7201 ttgatctgat cgcgataata agctgctttt tcgaaatctt cttctcgaga agctaaaact
     7261 ttttgttctt ctgcttcttg cagttttttc tcgattgttt taggatcaac gatttggatc
     7321 gtcaagtttt tcttagaacc tgtttcatct agcaaatcga tggctttatc cggcaagaag
     7381 cgatcttgga cgtaacgatt ggataaagtt gcggctgctt cgatggcctc atcagtatat
     7441 tttacatgat gatagtcttc ataacgtttt tgcaaacctt tcaaaatact gatcgtttct
     7501 tcgacagttg gttcatctac ccgtacaggt tgcatacgac gttctaaagc agcgtctttt
     7561 tcgatgatgc ggtactcatt taaggtagta gcaccaacca tttgcaattc tcctcgagct
     7621 aaagctggtt ttaaaatatt accagcatcc atatttccat caccagcaga accagcaccg
     7681 acgatttcgt gaacttcatc tatgaatagg atcacatttt ctgcttgacg gatctcttca
     7741 atcaattttt gcatacgttc ttcaaattgt cctcgtatac cagtaccttg gactaaagat
     7801 acgacatcta aacgaatgac ttctttgtcc attaattttt gcggtacatc accatctacg
     7861 attttttgtg ctaaaccttc aacaactgct gttttaccaa cacctggttc tccaatcaag
     7921 acaggattgt tcttggttcg gcgatttaaa atttcaatga ctcgtttgat ttcgtcatcg
     7981 cgtccgatca caggatcgat atcaccttgt cgtgcttgtt ctgtgatatt gattccgtat
     8041 tctcccaata aaccttctgt attgccttga ggtggctgac caccattgaa gttgttgttt
     8101 ccgccaaatt gtgttggtgg tgtttgctca taatttcctt gctgttgcat ttggcgagac
     8161 atggaacgga aaagatcgtc caaactgcca aatccaaatg gatcattttg ggccatatta
     8221 gtcagacctc cattttgttg atttttgatt ttttgataac agctctgaca gtaatcgagt
     8281 tgtgtccgct gaccattgac agtggcatac aagtgaatcg ttgcctcatt cttgccgcag
     8341 ttttgacaaa gcatgaatca ttcacgtcct ttcacaaaaa tgggtacatc catattttac
     8401 atctcgtttt tcatgaggta aaagaataac ccctagtttt ttaagagatt tatgaggtag
     8461 aaaaggtgaa atctctattg accattcctg acctttgact cttattatac taaaaataaa
     8521 aagggaagca agtaattcgt ttcatatgaa aacaaagagg gtcaaaggaa agaatgataa
     8581 acgtcaggag aagagaaagc gacaagaaaa aggaaataaa cacgtttgtt ataaaatgtg
     8641 aagaaaagga tgaaaaagaa tccggaataa tttgaatact tttatctgaa attgaatgaa
     8701 aactgttgta tctaacgaaa aaaaatgtta atctttacag atgaaagggt ggcatttgtt
     8761 aggattctct gaattactaa aaaagaaaac cctcacataa aaacaacact cacttaaagg
     8821 agaccgatta atatggaaaa gaaagaattt cacgtagtag cagaaactgg gatccacgct
     8881 cgtccagcta cactattagt acaaacagca agcaaattta actctgatgt aaacttagag
     8941 tataaaggta aatcagtaaa cttgaaatca atcatgggcg ttatgtcttt aggtgtaggt
     9001 caaggttctg atgttactat tactgcagaa ggtgctgacg aagctgatgc aatggcagca
     9061 atcgttgaaa caatgaagaa agaaggctta tctgaataat ggttgaaatg ctaaaaggga
     9121 ttgccgctag tgacggtgta gccgttgcaa aagcttacct gctagttcaa ccggatttaa
     9181 cattcagtaa ggcaacagta gaagatactg cggctgaaga agctcgtttg gatgctgcat
     9241 tagctaaatc tactgaagaa ttgcagcaaa ttcgtgaaaa agcagcgcaa agcttaggtg
     9301 aagcagaagc gcaagtattt gatgcccatt tgatggttct ttcagatcca gaaatggtag
     9361 gtcaaatcaa gcaaaatatt aaagacaaca gcgtaaatgc tgaatcagcg ctaaaagaag
     9421 taacggatat gtatatcggt atgttcgaag caatggaaga taatgcttat atgcaagagc
     9481 gtgctgcaga tattcgtgac gttgctaaac gtattttagc gcacttatta ggcgttactt
     9541 taccaaatcc atcaatgatc aacgaagaag tggtcgttgt tgcacatgac ttgactccaa
     9601 gtgatacagc acaattagat cgcaattttg taaaagcgtt tgtaacagac attggtggac
     9661 gtacttcaca ttctgctatc atggcacgtt ctttagaaat tccagcaatc gttgggacaa
     9721 aagaaattac tgctaaagtc aaagaaggcg atattttggc tgtcaacggg atcgaaggcg
     9781 atgtaatcat cgatcctaca gatgaacaaa aagctgaatt tgaaaaagca ggtgcagatt
     9841 acgcagcaca aaaagcagaa tgggaaaaat taaaaaatgc agaaactgtc acagctgacg
     9901 gcaagcattt tgaattagct gcaaacatcg gaacaccaaa agatctagtt ggtgtccata
     9961 ataatggtgg agaagcagtt ggtctgtacc gtactgaatt cctgtatatg gattctccag
    10021 acttcccaac agaagatgac caatatgaag cttacaaagc tgtattagaa ggtatggaag
    10081 gaaaacctgt agttgttcgt acaatggaca tcggtggaga taaagaacta ccatatctgc
    10141 aattacctca tgaaatgaat ccattcttag gatatcgtgc attgcgtatc agtttatctg
    10201 aacaaggcga cgaaatgttc cgtacacaaa tgcgtgcatt gcttcgtgca tctgttcatg
    10261 gtaatttacg tatcatgttc cctatggtag ctacactcaa agaattccgc gcagcaaaag
    10321 cgatctttga agaagaaaaa caaaaattga taagtgaagg caaggaagta tctgatacga
    10381 tccaagtcgg catcatgatc gagattccag cagcagcagt cttagcagac aagtttgcaa
    10441 aagaagtaga tttcttctct gtaggtacga acgatttgat tcaatacaca atggcagcag
    10501 accgtatgaa cgaacgcgtt tcttacttgt accaaccata taacccatca attttacgtt
    10561 tgatcaaaaa tgtaattgat gcagcacacg ctgaaggaaa atgggcaggt atgtgtggag
    10621 aaatggctgg agatcaaaca gctgttccat tgctagtagg tatgggtcta gatgagttct
    10681 caatgagtgc gacatctatt cttaaaacgc gtagtttaat gaaacgcttg gacacagcga
    10741 aaatggctga acttgctgat cgtgcattga aagaatgcga tatgatggaa gaagtcgttg
    10801 aacttgttca tgaatatgta aaataaacaa cgtatcaaaa gctggtgtgg cttgtaaaag
    10861 agctacgcca gtttttttgt tcttttttaa gaaaacgttt ttgtcagtaa gaatagacaa
    10921 aa
//
LOCUS       DAYYCY010000085        10918 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000085 DAYYCY010000000
VERSION     DAYYCY010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10918
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.085"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            171..1430
                     /gene="lysA"
                     /locus_tag="ACHAZB_001773"
     CDS             171..1430
                     /gene="lysA"
                     /locus_tag="ACHAZB_001773"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFM8614419.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1417..2217
                     /gene="dapF"
                     /locus_tag="ACHAZB_001774"
     CDS             1417..2217
                     /gene="dapF"
                     /locus_tag="ACHAZB_001774"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFM8614420.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDTDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2220..3101
                     /gene="dapA"
                     /locus_tag="ACHAZB_001775"
     CDS             2220..3101
                     /gene="dapA"
                     /locus_tag="ACHAZB_001775"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFM8614421.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3098..3814
                     /gene="dapB"
                     /locus_tag="ACHAZB_001776"
     CDS             3098..3814
                     /gene="dapB"
                     /locus_tag="ACHAZB_001776"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFM8614422.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3820..4515
                     /gene="dapD"
                     /locus_tag="ACHAZB_001777"
     CDS             3820..4515
                     /gene="dapD"
                     /locus_tag="ACHAZB_001777"
                     /EC_number="2.3.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HFM8614423.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4531..5532
                     /locus_tag="ACHAZB_001778"
     CDS             4531..5532
                     /locus_tag="ACHAZB_001778"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFM8614424.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5529..6890
                     /locus_tag="ACHAZB_001779"
     CDS             5529..6890
                     /locus_tag="ACHAZB_001779"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFM8614425.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6850..8001
                     /locus_tag="ACHAZB_001780"
     CDS             6850..8001
                     /locus_tag="ACHAZB_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFM8614426.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSGAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            7976..9145
                     /locus_tag="ACHAZB_001781"
     CDS             7976..9145
                     /locus_tag="ACHAZB_001781"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFM8614427.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9320..10699
                     /locus_tag="ACHAZB_001782"
     CDS             9320..10699
                     /locus_tag="ACHAZB_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM8614428.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
BASE COUNT         3623 a         1910 c         2440 g         2945 t
ORIGIN      
        1 ttcgggtatt ttgtttttat aaccattttt attcttattt ttaaaaaaat atttcgaaat
       61 atactagaca attcagaaaa ttttgtttaa tattattcca ataaaattca aatctttatc
      121 aaatgaaatt ccaataaaaa cggaaaaaaa gtgaaaagag gaatgaaaaa atgacagaat
      181 tgataattgg cggagtcaca gcaagcacat tggcagaaaa gtataagaca cctctgtatg
      241 tgtatgatga agaaaagatg agacaaacga tgctgtcatt caagcgaggc tttgtatcga
      301 aacgttttga tacaaaagtg ctttacgcct ctaaagcatt ccaaacgatt gagatgctgc
      361 atttagttcg tgaatatgga ttcgggctag atgttgtcag tggtggggaa atctataccg
      421 ctttgcaagc tgattttcct agagatacta tctacttcca tggtaacaac aagacgccag
      481 aggaactgat ctatgcactt gaaaatgatt tgttgcatat tgttgccgat aatttgatgg
      541 aagttgaatt attagcagaa ttaagccaac gctatttaaa aaaaatctgt atcatgcttc
      601 ggttgaatgt cggagtagaa gcacatactc atgaatatat cgtcactgcg catattgatt
      661 caaaattcgg catgagctat gaaagtaaag agtgccaaca agcccttcag ttgataaaag
      721 aaagcagcta tctagagtta gaaggatttc atgctcatat cgggtcacaa atttttgatg
      781 ttactgcttg gttagctgaa atcgacaaat tgacgaatta cttaaccgat tttgaccaac
      841 ctctttcctt gaatcttggc ggagggtttg ggattcgtta tacacaaaat gatcagccga
      901 tcccagtcga agaggcacta aaaaaactga ttgaatatac ggaagaagct ttggaaaaac
      961 gaaaactgac cattcgtcaa ttactgatcg aaccgggtag aagtattgtt ggagaagctg
     1021 gtacaacact atacacgatt ggtttcatca aaaagacacc acataaaaaa tattactttg
     1081 tagatggcgg gatgacagat aatatccgtc cagctctgta tcaggcagaa tacgattgtg
     1141 atttagcagc aaaactgtcg gaagaaaaaa cagagaaagt gacgattgca ggaaaaatgt
     1201 gtgaatcagg cgatgtggta attaaagaag cttatttgcc gaaagcagaa tcgggtgatc
     1261 ttttagctgt ttattccacg ggagcttatg gatactcgat gagcagcaac tacaatcgag
     1321 cgacacgacc agcagttgtt tttgtcaata atggccaatc aagactgatt gttcgcagac
     1381 aagcattttc tgatctacta aggggggaag tttcctatga tccttcataa atataatggc
     1441 tgcggaaatg attttatctt attagattac gcagaagaca cagattatag tcaattagcc
     1501 gcttctttat gcacaaaaga aaaatatgat acagacggat tgatcgccgt aaaaatcgat
     1561 ccgttggaaa tgatctatta caacaaagat ggcagccgcg cgccgatgtg cggaaatggc
     1621 attcgctgtt ttgctcgata cgtaaatgat cagaacatga tgaatgaaca ggaattcaac
     1681 gtagagacat tagcaggcac aagaaaaatt acggtgttgg atccctcacc tttttattgc
     1741 agcgtagata tgggagtgcc agatgattct ccggaaaaaa tcaaagctaa tcaaacttat
     1801 ccaataaaaa accaggaaat agagatcaat ggtgaaaaag taacgatcac tagtttattt
     1861 ttaggaacga ttcatacagt tgtttttgtt gaaaatgcgc gtgaggaagt ctggagaaaa
     1921 agaggtgaac tgatctgtca tcatcctttg ttcaaagaaa aaaccaatgt caactttgta
     1981 gaagtagtca atgaacaaga attgatcgtc cgtacctatg aaagaggagt aggttggaca
     2041 cttgcttgcg gtacaggttg ctgtgcctct tatgtggttg ctaaggaaac tgggaaaatt
     2101 tcagcagatc aggtagttgt tcacttggaa caaggagact tgatgatctc tggtgaggat
     2161 gcgatacaaa tggctggccc agcagttcat gaatggacaa gaatattgga ggaatgaaga
     2221 tgataaaagg ttcgatcgtg gcgctgatca cgccgatgaa tgaagaggga tcagtagatt
     2281 atgctggttt ggaaaaattg atccaattcc atttagatga acaaacagat ggattattag
     2341 ttttaggtac aacaggagaa tcttctacgc tcacacaatc tgaggaggag cagatccttc
     2401 aactgacagt aaagaaagtg gctggccgtg tgccggtgat tgcaggagct ggaacgaata
     2461 atacgaaaga aacgattgaa aaagcgaagc attttgcttc tttgggggca gacgcacttt
     2521 tggtcatcac cccttactac aacaaaacaa gtgatgcagg actagctgct cattttacag
     2581 caattgcaga agcctcaccg attccgctta ttttgtataa tgttccttct agaacaggca
     2641 tgtcgattcc aatccatgta ttagtaaatt tagcagaaca tcctaatatc attggtttga
     2701 aagaagcctc aggagatatg gcttatgtga tggatgccgc tcggttgatt ggagaagaat
     2761 tcttccttta cagtggaaat gatgatttga tcctaccagt aatgagtgta ggaggaagcg
     2821 gtgtaataag tgtatgggcg aatatccaac ctaaaatagt ccatgaatta gtaaaagata
     2881 cgcaggacgg aagatggcag caagcaaaag aaaagcaatt aaatgcactt gaactgatcc
     2941 atgccttgtt cagtgagacc aatcctattc ctgtcaaagc agccatgtca ttattagact
     3001 taccttccgg acctctacgc ctgcctttag tttctctttc aaaagagaaa aagaaacaat
     3061 tagctcagct tttgttaaaa gaaaggacgg ttgaaaaatg aaaatcggac tgattggcag
     3121 tggacagatg ggaaatagag tcgaagaagt tgcacaagaa agaggagata cggtccttct
     3181 tttacggacg gcacctaaag aagcaactac gcttgctttt acggaactgc cagaactgct
     3241 tattgatttt tctcatccag ataatttaga gaaaatcctt tcgtatagct tatcatatca
     3301 acttccgtta gtcattggca cgacaggtta tacacaatca cagttccaag aaataaaaga
     3361 acatgccaaa catgtacctg tgatgtatag tgctaatttt tcgttcggca tcatggtgat
     3421 gaatcaacta atcaagcaag cagctgcaat gttgcaaggc tggcagatcg aactgattga
     3481 aaaacatcat agtcgtaaaa aagatgcacc atctggtact gcaaatatgc tgcttcaaac
     3541 aatccaagaa gtgcaaaagc tgcagcccgt atacaactgg caagggaaaa aaagagaaga
     3601 aaaccaaatt ggtgtacaca gtattcgtgc aggttcttta cctggtgaac atgatgtatt
     3661 gtttgctgct actgatgaaa tatttacgat caaacatgaa tctttttcta atcgaatctt
     3721 tgctgcaggc gcagtagaag cagcggactg gttgaaagat cagtcaccag gttattacaa
     3781 attagaagat ttattaatgg acaaaggggt gtaaaaaacg tggaagctca agaaatcatc
     3841 cgctacattc aaacggcatc taaaaagacg cctgtgaaag tctatctgaa tgtaaccgag
     3901 ccaatcgaat ttaaagaaag taaagttttt ggtgaaggaa gcagcttcgt tgtttttggt
     3961 gactatgaga cgattgctcc ggttttagaa gctgaatcag aaaaaatcat tgaaatcgaa
     4021 atcgaaactg ctgcaagata cagcgcagtt cctttgctag atattaaaaa aattaatgct
     4081 cgtatcgaac caggtgctat tatcagagat caagtgtcaa tcggcaacaa tgccgtgatc
     4141 atgatgggag cgattatcaa tattggtgca gtaatcggtg aaaacacgat gattgatatg
     4201 ggtgctgtct taggtggacg agcgacagtg ggcaaaaact gccatattgg tgcaggagct
     4261 gtcttagcag gtgtgatcga accagcatcg gccaaaccgg ttatcgtaga agacggcgta
     4321 ctggtaggtg cgaatgcagt gattgttgaa ggtgtccata tcggaaaaga tgcagtcgtg
     4381 gcagcaggag cagtggtttt agaagatgtg gcagcagaaa ctgttgtcgg tggaatacct
     4441 gcacgtgtat taaaaataag tgatgatcag acaaaagaaa atacagcatt gattgctgcg
     4501 ttacgcgaat tatagaggag atggagaaaa atgcgcgaaa tagatacctg tattgtgggg
     4561 gcaaccggat tagtcggcca aacgatgctc aaagtgttag aagaatatga ttttccagta
     4621 ggacgattga aattattagc ttcaaaacgt tcagccggta aagtttgtgt attcaagaaa
     4681 aagaaacaca ctgtacagga attgacagaa acttcatttg aaggatatga tttagcgcta
     4741 ttttctgcag gtgcttctgt atccaaagag tttgcgccaa tcgcacgaag tcagggacta
     4801 tttgtcatag ataattcctc agcatggcgg gaagattcgg atattccatt aatcgttcca
     4861 gaggtcaatc tggaagacta tacattgaat cggctgattg caaatccaaa ttgttcaacc
     4921 attcaagcgg tacttccgct taaagcttta caagaagcct ttggtatcca gcgagtcaat
     4981 tattcgacct atcaagcagt ttctggttcc gggcaaaaag gcattgaaga tcttcatcgt
     5041 acgcaaaatg acgaggaacc agttttgtat ccttataata ttagccaaac ggtgattccg
     5101 gaaatcgatc agcctttgga aaatgggtat acaaaagaag aacagaaaat gataaatgag
     5161 acccgtaaga ttctgcatca gcctgctttg ccagtatcag caacctgcgt acgtgtgcca
     5221 atcgaaaatg gacatggagt ctctgtggca gttcaactgc aaaaagagtt tacgacggaa
     5281 gaaattagag agtgtctcag ccagtttcca gggattcaat tagaagatga tctagcagca
     5341 cataaatatc cgacgagcat tcttgctaga ggaacagata tggtttatgt tgggaggatc
     5401 agaaaagaca cttctttaga gaacggattg ttgttctata cgactgccga taacatacgc
     5461 aaaggagccg ctgcgaatgc cgtacagatt gctgcagctt taatgcagaa aatagcggag
     5521 gtggcaagat gagtaatacg ctgaaagtag cgaaattcgg cggaacatcg atgtctcatg
     5581 aagagcaatt caaaaaagtc cgtacaattg ttctttcaga tcccaatcgc caagtgatcg
     5641 ttgtttctgc tttaggcaaa cgtacgtcag atgatgacaa agtaacagac ttactttatt
     5701 tgatttatgc ttaccttcag catcatgttg attggacacc cctttggaaa aggatttgtg
     5761 atcgtttcat tcaagtaaga gatgttcttg ccctgcagtt acctattgaa gatgaactag
     5821 cacgcataca gaaacaactg gaagacgaaa agataactga agattattta gtaagtagag
     5881 gtgaatatct gactgctcaa ctgatggctg agtatcttgg ttaccagttt attgacgcaa
     5941 aagacctgat tttctttgac tatgatggga aaatcgatca ggaaaaaaca gcacaggaaa
     6001 tcacaaaagc atttaaaaga tatccgaaaa tggttgttcc agggttttac ggggcgaacc
     6061 ctgcaggaaa agaaaaatta ttgggccgtg ggggaagcga cattaccggc gcgatactgg
     6121 cgaagatgtt gcaagcggaa agttatgaga attggacgga tgtttcagga atcatgatgg
     6181 ctgatccgcg aatcatcgat catcctaaaa tcatcgagga aatctctttt agagaactga
     6241 gagagatggc ttatatgggg gctaacgttc ttcatgaagc agctgttttc ccagttcaag
     6301 aggctgatat ccccatccaa ataaaaaata ccaatgatcc tttcgctcaa gggacaaaaa
     6361 tatcgaatca cgaaatagaa aaggaaaatg gactgacagg aatagctgga cgcaaggatt
     6421 ttctttccat cactttattc aaacgtcata tgtccgatga aatcgggttt atctggaaag
     6481 caatgagcat ttttgctaaa catgagatat caatcgaaca tattccttca gggatcgaca
     6541 atatcggagt gatcgtctca gcagaagcga ttgccgataa gttatttctc atcacgaaag
     6601 aattaaaaga aacacttggc gtggaagaaa tcgtcgtgat cgaagactta gctttgatct
     6661 ctgtcgttgg gggtccgcat aaagaattga ttggattatc tgggaaagtg ttatcgatat
     6721 tgaacaaatt agaaattcgc acatcgatct tatcacaagg agcacaggag ttgaatctga
     6781 tcatcggtgt accgaataat caatatgaaa cagtagtgaa gggtatctat gaagggatgg
     6841 tgaatacgga tgctgaaaga atatcgaatg gcactccatc aaattcctga aatcgggttt
     6901 catgaattca agacaaaaaa atatttatat gaacaagtaa aaaacagcgg ggccgttatc
     6961 catgaaatcg gcgaaacagg actactcctt tattttgata atcagcaaga agaaacgatt
     7021 gcttttcgaa cagatatcga tgcactgcca gtcaaagaag aaacgaatct ctcttttgct
     7081 tctaagcatg aaggttatat gcatgcatgt ggtcatgacg gacatatggc gatgctttta
     7141 gggttgactg actttataac taaacacatt gaggatctta tttataatgt agtactgatc
     7201 tatcagccat cagaagaaat ttccggtggc gcacaaagcg ttattcagtc aggattgttg
     7261 gataaatatg aagtacaagc aatatttggc tttcatctat ggcctggact agctaaggga
     7321 gaagttttct ctcgaccagg acctttgatg gcacaaagca gtgaaacaga cattatcatt
     7381 aaaggccagt ctgcacatat tgcttctagt gaaaaaggaa tcgatagtct ggaggcagct
     7441 gtacgatttt tgaagaatgt ttatgatttt gaccggcaat taccggcaga gtggaatcat
     7501 ctaatgaaat ttggccaaat ccaaggtgga acgatccgca atgttttagc tggagaagtt
     7561 attgtatccg gaagtatccg ttcttacagt tcagatatgc aaaattattt gaagagggga
     7621 ttagaccatt tagcagaaca gtttcactct acagctacta tttcttttcg ctataatgac
     7681 ggctatccag ctgtaatcaa ccatagaggc ttatatgcag cactgttaga ttcaaacaaa
     7741 ctacatgaat tagatgctcc agttcttcaa gccgaagatt ttggcatata tacgcaacaa
     7801 tatccatgtg tatttttctt tttaggtgtc ggtgataccc ctgctttaca tgatccgacg
     7861 ttcgattttg atatggatgt tttagaaaat ggtgtagaat ggtacaaaac gattcttttt
     7921 acagaaaatc ttcttgtaaa aatcgatagc agttcaaaag aggagcgagt aaataatgat
     7981 gaatttatcc aatcgattta gtcctaggct ttctagaata gaagtatcaa aaatacgaca
     8041 attcgatcag caaatatcgt cgataccaga tgtgatcaaa ctcaccctgg gagaaccgga
     8101 ttttccaact cctgaacatg tcaagcaagc aggaatagca gcgattgaag aagacttttc
     8161 tcactatacc ggaatgagag gattagagga actgcgggaa gcggcttgta tatttcagca
     8221 acagcgttat ggattaactt atgatccgca aacggaagtt ttgacgacag ttggtgcaac
     8281 ggaagcgatt gcctctgcgt tgttatctgt tttggaagaa ggagacaagg ttcttattcc
     8341 agcgcctgct tattcaggat atcagccatt agtggaacta gctggtgctg aattgatccc
     8401 gatagatact tctgacacag ggtttgtctg tcagccggaa caatttgaac gagcatttga
     8461 acaatatgga tcagctgtca aagcagtcat tttaaactat cctaacaatc ctactgggac
     8521 gacattgtct gcaaaacaat tgaaagcaat cgctgaggtt ttaaaaaaat atccggtatt
     8581 tgttataagt gatgaagtat atgctgaatt gacttatagt ggaacgcata tgtccatagc
     8641 tacttatctt ccagaacaaa caatcgtgat aagcgggtta tccaagtcac atgcgatgac
     8701 tggttggcga gtgggcttta tttttgctca aaaagccctt attgacgaac taattaaagt
     8761 ccatcaatat ctggtgactg ccgctacaac gatgtcgcaa aaagctgcgg ctgaagcgct
     8821 aataaatggg gtgaaagata gcgagaatat gaaagagcaa tatcaaacac gaagagacta
     8881 tctggtccag caattggcgc ctttaggatt tgaagtgacc cagccaaacg gtgcatttta
     8941 cttgttttgc aaactcccag ctgccattca aactgactct tggcagtttt gtgtagaatt
     9001 agcggaacag gcaaaagttg cgtgtatccc aggaattgca tttggcccag aaggagaagg
     9061 ttatatccgg atttcttatg ctagcagcat ggaaaaatta catgaggcct gcaaacggat
     9121 caaagacttt ttaacagaac agtgattcat agattttatc aaaaaaacaa tcgttaattt
     9181 tgcggtatat tctgcaagga gacgattgtt ttttaaattt agttcaaatg aacgcgtttc
     9241 ttttgattca taactaatgt aatgttaagt ttaaattgtt tagaatgatt ttaaaagaat
     9301 attcaaggag gtttcatcaa tggaaaaaat caaagttgtc attgtaggtg catctcatgg
     9361 agggcatcaa tcgatcttgg aactattgac acgatacggt gaaaatgtag atatcacgct
     9421 atttgaagca ggagactata tttccttcat gtcatgtggt atggagctgt atttagaaga
     9481 caaggtaaca gatgtaaatg atgtacgtaa tttcagacca gaaaactttc cgcaatccaa
     9541 tgttcatatt ttaaataatc acgaagtaaa aacgatcgat gccgataaaa aaacagtaac
     9601 ggttactagc gcatcagata acgaaacaaa agaatatcct tatgacaaat tgattttgag
     9661 ttctggtgtc aaaccaaaca atctgccagt accaggagca gatctagaaa atgtctactt
     9721 gatgcgtgga tacaattggg caaccaaaat caaagaaaaa ttaaatgatc cggctattaa
     9781 aaaaatcgct gtcgtaggtg cgggttacat tgggatcgaa gcagcagaag caagccgaaa
     9841 agctgggaaa gaagtcgtat tgcttgatgt gatcgataga cctttaggca cttatttaga
     9901 tgctgagatg acagatattt tagaacaaca cctaaatgaa aacggtattg aagttttgac
     9961 aaatgcaaaa atcgaagctt ttactggtaa cggaaaagtt gaggcaatca aaacaagtga
    10021 aaaagaaatc ccagcagatc tagtcattca agctgccggt gtgaaggcaa atacagaatg
    10081 gttaaaaggg atcgtcgact tagatgaacg tggctggatc caaacaaatg aatacttgca
    10141 aacgaatctt ccagatgtgt atgcagtagg agatgctaca cttgcttatt ctatcccagc
    10201 acgcaaaaaa atgccgatcg ctttagcgac tgttgcacgt agagaagcac gttatgtagt
    10261 aaaacatctt tttgaagaag taccaaacaa accgttcggc ggagtcgtag gttcctctgc
    10321 attaagtgtc tttgactatc attttgcagc aagcggattg aatagcttta cagcagcaaa
    10381 agcaggagta gatgtacgag tgtcttatta tgaagatacg ttacgaccaa aatatgtacc
    10441 agaagaacaa ggaaatccaa aagtgtccat ccaattattc tcggatccaa tgacccatca
    10501 attgctagga ggagcagtat tatctaccta tgatgttaca ggtcaaggaa atgtattggc
    10561 actagcgatc caaaacaaac tgactttaga agaactagca gaagctgact tcttcttcca
    10621 gccaggcttt gaccgacaat ggagcatgtt gaatctagcc gctcaacaag cgttaggaga
    10681 agaaccattt gtagaataag aaaacgaaat atttgaaaag aggttgtgaa aaagctgcct
    10741 tgcatcaagt aataagaaca gccaagtaaa aacagcttct tatgttttta acttggctgg
    10801 acttattacc gcagatgcaa gcttttgaac accgtttatt ttcaaaaaag ggactgtgac
    10861 acgatttgtg taccgacccc caaaagttag accaaaaaat ctaacttttg gaggtcgg
//
LOCUS       DAYYCY010000086        10258 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000086 DAYYCY010000000
VERSION     DAYYCY010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10258
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.086"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(53..1561)
                     /locus_tag="ACHAZB_001783"
     CDS             complement(53..1561)
                     /locus_tag="ACHAZB_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HFM8614429.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATCLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            1720..1911
                     /locus_tag="ACHAZB_001784"
     CDS             1720..1911
                     /locus_tag="ACHAZB_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8614430.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            2114..3031
                     /locus_tag="ACHAZB_001785"
     CDS             2114..3031
                     /locus_tag="ACHAZB_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HFM8614431.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            3024..4097
                     /locus_tag="ACHAZB_001786"
     CDS             3024..4097
                     /locus_tag="ACHAZB_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8614432.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            4245..4763
                     /locus_tag="ACHAZB_001787"
     CDS             4245..4763
                     /locus_tag="ACHAZB_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8614433.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            4744..5136
                     /locus_tag="ACHAZB_001788"
     CDS             4744..5136
                     /locus_tag="ACHAZB_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HFM8614434.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVVFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            5212..6147
                     /locus_tag="ACHAZB_001789"
     CDS             5212..6147
                     /locus_tag="ACHAZB_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFM8614435.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            6280..7059
                     /locus_tag="ACHAZB_001790"
     CDS             6280..7059
                     /locus_tag="ACHAZB_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFM8614436.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            7052..7369
                     /locus_tag="ACHAZB_001791"
     CDS             7052..7369
                     /locus_tag="ACHAZB_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFM8614437.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            7613..9223
                     /locus_tag="ACHAZB_001792"
     CDS             7613..9223
                     /locus_tag="ACHAZB_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFM8614438.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            9380..10228
                     /locus_tag="ACHAZB_001793"
     CDS             9380..10228
                     /locus_tag="ACHAZB_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFM8614439.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT         3224 a         1799 c         2070 g         3165 t
ORIGIN      
        1 actatcgatt aagctaatag tttcgttttc aacatttata agtaaacttt ttctattggt
       61 aatatagatt ttcggaaggg tagacaggtg cgcaggtaat atcgattcct agttttttca
      121 atgtttgttc atcattttta ttgaccatca cagtagagtg ggcttgtgct ccctcaagtt
      181 cagctagttt atcataagca acttgagcga ttggattcgt tactgcactg attgataaag
      241 cgatcaagac ttcattgatg cttagtgcat ccaaagagga atgaagtgtc tttgatttca
      301 atccttgtat tgtttcaaga actaatggtg acaataaagg aatgtcatct gcgatatggg
      361 ctaaatgttt tactgcattt aagataactg ccgctgacgc atccatcaat gaacttgtac
      421 gcccggtaat gatgacttca tctggtaatt cgattgcaat cacagcagaa ggatcttttg
      481 taggactttg cgcttgaatc ttatctgcat agtcatgggc agggactacc acgtttcggt
      541 cttcaggacg taaaccgact tcttccataa tcagtttgat tcgattaact gcttcttcgt
      601 cgatcaatcc ttttttgaag tcgcattctg tttggaaata acgacggatg atttcttgtt
      661 tagaggcttc ttggacaacg gtatcatcca cgatcccaaa tcctacacga ttcacaccca
      721 tgtcagtagg agattgataa acagattctt taccagtaat tttttcaata atacgtttga
      781 ttacaggaaa tgtctcaaca tcccgattat agttcacagc tactttattg taggcatcaa
      841 agtggaagga atcgatcata ttgacatctt ttagatcgac tgtggctgct tcataagcaa
      901 tgttcagcgg atgtttcaac ggaacattcc atactgggaa agtttcgaac ttggagtaac
      961 cagctgcatg tcctttttgg ctttcatgat acagttggtt caagcaagta gcgagctttc
     1021 cacttcctgg gccaggagct gtcactacaa cgattggttt agttgtttca atatactcat
     1081 tttttccaaa accatcttct gagacgattt tttctacgtt gactggatag tcttcgattt
     1141 ctgagtgttt atagaccttg atgtttcgtc tttctagttt attgataaag actttcgtgg
     1201 caggctgtcc actatagcga gtgataacga cactgttgat taataagcca taaccacgta
     1261 attcatcgat taaccgcaag atatccatat cataagtaat cccataatca ccacggatct
     1321 tattacgttc aatatctcca gcatagacgc aaatcagtat ttcagcttga tctttcaatt
     1381 tttgcagcaa tttgattttc gcatcttctt caaatcctgg aagtacacgt ttcgcatgct
     1441 tgtcgtcaac taacttccca ccaaattcta aatataattt gtcatagtgg ttcactcgtt
     1501 ccaaaatata cgctgactgc tcttcaatat atttttctga atcaaaacca atttttttca
     1561 ctaggttccc tccttaagta ctaaagcatt ttttcatttt tttgaggatt cgtcaactgg
     1621 aagagcgaaa ttcctcaaaa aaacaaagaa aatggtttaa accaacttgt ctagatagtt
     1681 attcattggt atgatagttt ttgataagga aggtgctaca tgaaaaaaag acagaaaaag
     1741 aaaaacgcat ataaacacta tatccgttcg atttttaccg gttatgaaag aatgcttgaa
     1801 gatcccgaat tggaacaatt gacgtttact tatctaaacg aagaaacgca gctaactcgt
     1861 gatgagcatc agcgcattca tttcacaaca agagatctgc ctagtaaata aaaaataaga
     1921 taaaggtcat cggattcgat tctgtcctat cagatggaaa gactagttga tcgaaaaaaa
     1981 ggctgtacca aattttggtg cagcctgttt tatggagcat tgcccgctct tttttataac
     2041 aattttttac agaaaatggt actattgaag tattaataag tgaaaaacag atgagtaaga
     2101 ggaggcaagt agtatgaaag agccagatac aaatgcaaat tatgagccag acgaactaga
     2161 ggagacgaac gcggatcagg atacaggaca agaatcgtct tctttttacg atatcaaaga
     2221 aaaagcaagc atgaatcttt cccaattatt agaagactta caggcttttg aaaaagctgt
     2281 ccgcgaagaa gatatccctg agatctaccg tttatacaat ggccgattga atgaagaatt
     2341 aaaaagaagc tcaaatgaaa atcacgagat cgatcaattg ctgatgcgta agatccatga
     2401 tcgtttttta caggaatttc cttttatgga acaagtagaa aacatctcgc caacgatgag
     2461 ctattacaaa atcggcactt attaccatga tcggcccacg gtcggtattg atgcaagtct
     2521 gccagaaatt tttgttttgc ctaagatcga tgaagagtgg gagaaatatt cccaaccgga
     2581 gtcaacggac tatgacaaaa aaatcaatga gctagacgca aaggtgatca ctgctcagac
     2641 agaaattgaa cgtttagatg aacaaataaa agaaatcaat cgacaaatga ctgatttgaa
     2701 cgacaataaa ggattcttga atcgtaaaaa aatcgaggaa gaaattcaag aattagagaa
     2761 gaagaagcaa gtactatcta atgaaaaatt aggctggctt ccttatattg agacgcctga
     2821 aacaatccag cagcaaaaag aagcattgaa gcaagaagca agagcagacc agcttcgtgc
     2881 agccatcgta gaaaaagaac aaaggcaaat cagacgctat ttcggtagca aagagggatt
     2941 cggtcaagca atccatgaat tcttgatgaa ttatcttggc aatgagaatc ctaataatca
     3001 atctaatgaa ggaggttccg agtatgaatg aacacgatga agcagaatgg acaaaaaatg
     3061 aagaacaaac agaagatcat gaagaaacga acacttcatt ggaagaacaa acaagtaatc
     3121 ctctggatga cacagaagaa atccctatgc tagaggaaac aaaaatacat gaaaaagaaa
     3181 aagtaactcc gacgaatacg aatactgcag aaaacaccca tgcttacgaa ataactgaaa
     3241 atcccgggta tttagaagga aaagagcaag atcatcaaga tatcgtcagt ttgattgaaa
     3301 cagcggaagc agaattagcg gatttacgta tgagaaaaga tttgatcgaa tcagattttc
     3361 cacaattatt agaatcatat gatcgtttta tattgaatga agaaccaatc tccacgatcg
     3421 gaaagacaag cctgatcgaa tacttcacgt atgaattact gaaaccgttg aatgatattg
     3481 gtctggaacg gtcaaattca gcttatgaca catggaaaac aagacatttt tcgatcaatt
     3541 atacgttgaa tggacaaaac gaattagtct tttcctttgt attaccgacc atcgatcaac
     3601 gataccaagt ggaatcgatg agtctcctaa acgtatcacc agagacaatg gaaatcaatg
     3661 tccaagatga ccgggtgctt tctttgatcc gacactggtc agtggatcga gtattctctg
     3721 cagggcagat cacgatattc aatcacaaat tgaatcaatt attagcccat gcaagaacac
     3781 ttggttttac cgttaatcag acattactag acaatacgaa gccacttcgt ttgaatcttc
     3841 agagcgaatt tgaattgacg aatgaagtat tggatgatat ttttatcgtg gcaatgaaaa
     3901 atccatccta cgatatggaa aagatcaacg aaactactta tcaagtacga ttagataaag
     3961 gacagtcttt aacgataaca acaaacgaaa aagatcaaac agtcttgtcg atctcatctg
     4021 gagactaccc acgatctgtc attgatttct ttatcaatta cgaattcttg gtcccattaa
     4081 tggtgagaaa gtcctgattg gataagttgg attaccagat gtcattcaga taattctgtt
     4141 ccaaaggact tgcggcagtt atccagaata attgtcacaa gtcttttctg tatgtactaa
     4201 atggacgtga ataacttgct gatgaaggag ggatttcatt aggaatgtcc acaaataaaa
     4261 tagcacagct tagcttattg tctgctgctt gtgtagcagg taggatcgct tttcaattta
     4321 ttccgaattt ccagccaatg actgctattt ttttatttat agtactttac ttaagcttaa
     4381 aagatgcgtt aattgtcatg agtctttcta ttgcgatcag cagtttttat tttggtgttg
     4441 gaccatgggt catcggccaa tggatcagct atactgttgt tctgagtttg ttttcaatcg
     4501 tctgcttgag aaaaacagtg caaaggaatc tatggttaaa gggagtctgt ttttttctgg
     4561 cgggcatggt ttacggaata ggtatgaccg tttttgatac gctgttatat cgtttgcctc
     4621 aaccttggat ctattacctt caaggggtgt cgtttgattt gatgcacagt ataggcaatg
     4681 ttgtgttctt tcttgttttt ctgccagtcg tcaaacgttt ttacaatcat tcaggaggaa
     4741 aaaatgaaaa aaataattta tagtttgctt gcaatgagtg tcagtatact cattttgaca
     4801 agttgcagta caacctatac acaatcggct tccaaccaaa aggaggaagt aaaaacatcg
     4861 caaaaagcaa caatcacgtt acaagaaaat ggtcaaaact tttcggaaaa agaagtggtc
     4921 ttcaaaaaag gcgatgattt actgagtatt ttgaaacgaa attttgaagt caaagaagat
     4981 aacggcttca tcacttcgct tgaagggcac tcacaggatg agaaaaatca actctattgg
     5041 atgtttactg tggacgggaa aagtgctact actggtgcca aagagatcaa attgaatgat
     5101 ggacaatcgg tcattttcaa tttgtctaaa ctataagttt cataaaaaac tgttaaaggg
     5161 cgaatattgc cataattcag tgagttcagt tgatgaaaga aggaaaataa catggaaaat
     5221 tttaaaaaaa gcggaatgtt ttccgtaatt gctgctctag gggctaatat tctagttgct
     5281 atcagcaaat ttgtcggcta tgcaatcagt ggcagtgccg ccatgctaaa cgaaagcatc
     5341 cacagtatcg ttgattgcag caatcagatt ttgctgttat tcggtgataa acaagccaat
     5401 aaaggacaga gcgagttgca tcaatttggt gaaggacgag caaaatactt tttcagtaca
     5461 atcgtcgcaa tgatgctttt cttcggcggt ggggcacttg gggtgatgga agcttttgaa
     5521 aagttgacgc atccatccca tgaagtgggt aatccgatca ttgttatagc aatactgtta
     5581 tttggtttgg taatcgaagg aagttcgtta cgcgtggcaa tgaaagaaat caaagaattg
     5641 aatactgaaa aactttccac gatgcgcttt ttgcgcgaaa gtcgacacag tgaaatactg
     5701 attattttta cggaagactt gtgtgctgta atcggtttgg tcttggcttt agcaggaaca
     5761 atattgactt atgcaaccgg gattgctgca tttgatgcga tcagcggatt gctgatcggt
     5821 ttcttgctga tgggagccgc cattttccta gcaaaagaat tttatagtct gatcatcggt
     5881 gaaagcgtga cagcaagcga tttatcaaaa atcaagttag cttttgaacg tcctgagatc
     5941 agtcggctga tcgatataaa aacagttcat ttaagtccaa cggagatttt agttgcagca
     6001 aaagcagata tcgttggtaa caaagagaat gaagcttact cggtcatcaa tgatattgaa
     6061 aaaaatatgc gtcagtctct tcctgacaag aagatgtata tatacattga aacagataaa
     6121 tatgacccaa attattctcg taaataaaac gtttattcat gaattgaagc aaaaatgaga
     6181 aaaatataat cttgttttta aataaaacgg ctacaattta gcgaatttat gttacaatga
     6241 gtccgctgtt tatttgttgt aattaaagga gatattttaa tgaacaaaaa gaaaagtgcg
     6301 ccatggcttc aagctttgca tgcagtgatg ccactttgta taagctatat accagtagga
     6361 ttagcttgcg gagtattgtt gcaaaaagtt ggatttgatc ctctcttatc aggactgtta
     6421 tcgattctgg tcttttccgg tggcgcgcag tttttagttg catctatgct tacgacgcaa
     6481 gcatcatttg caacgacatt attgatggtt ttctttttag aattacgcta tacattatta
     6541 ggagcaagtc ttgccggttt tatgaaaaaa gaaaagcgga cgtttttggc tgtcttttct
     6601 caatcactga atgatgaaaa ctatgcggtg aactacttaa agttttcaac tgacccttcc
     6661 tggaataaaa gaaaggcttt atatgtcaat tggttctcaa tgactgcatg ggccggtagc
     6721 aatatgatcg gtaatttttt cggctcggtg attcgagtag atgctgacct tgtccatttt
     6781 gctttgacgg ccatgttcat tttcatgttt atcatgcaga tgaaaagcgc actactgatc
     6841 tttacaggtt tattctcagg cgtattaggg gtcgtattca tggtattatt ccaaaatact
     6901 ttcggattga ttgcagcaac agtcattgct tcatttgccg gatttatgct ggagaaggca
     6961 ttcaaaaaag aaaaagctaa gaaactaaga gaccgcggta aagatgtcaa tgagccgctc
     7021 ttcaacttcc cggaacagga ggccaatcac catgactaaa ctatatttgc tacttttagt
     7081 tgcttgtctg ttcatcgtgg catatatccc gcgcttgttc ccgatgcttt attttactca
     7141 tcgaaaagtg ccatcctggt tcagtgactg gatgaaatat gtaccggtgg cattatttgc
     7201 ggcgttagct tttaaagatg ttttcattac tcatgaacat ctggatattg cttggaatat
     7261 caaaatagct gcaatgattc tagtagcagg cgtggcttac aaaacacgtt cgatggcgtt
     7321 gtcagttttg acaggacttg cgtctgtctt tttgctgtct atgctttaaa ctgttttatt
     7381 ttattaaaaa cagagattgc gacataagtt gttcaaactg atctatccga actgcatttg
     7441 caacagtcaa ttctggatgc aagtttattc gtagggataa aaaagattag tgttggctta
     7501 tgatgcaagc tcttttttat tttaggaatt agagagggat ttgtccttct atgatttttt
     7561 gctataatag ggaagatgtt tattttatga aagaatagtt gagggaattt aaatgaataa
     7621 aaaattgatg caaggaacat tttggctaac ctttgctaat cttctatgta aagttttagg
     7681 ggtagtctat ttgattccct ggctaagtat gatggggaac aatcaagatg gtatgcttgc
     7741 tacgacattg tataatgttg gctatcttcc ttatggattg ttcctgatgc ttggtacagt
     7801 cggatttcca aatgcgattg caaaaaaagt agctgtagcc acaaaagaag gagataacgc
     7861 tgcttgtcgg ctgattttta gaagtacgat caatatcatg tttgtcatcg gtatcgcctc
     7921 tgctgcactg atgtatttat ttgctccttt actagccgga atcagtccga ttgctaacgt
     7981 taataatggg atactagcta tcagaagttt atgcccatct ttgattgcta tcccaattct
     8041 tagtgcaatg agaggatatt tccaaggaaa aaatgatctg cggccttatg gaacttcttt
     8101 gattatcgaa caggctgtgc gagtgatcgt tatcttggct ggtacttatt acttgcgcgt
     8161 attgactgac ggaaccatat tagaagctgt attgatcagt acagtcgctt ctttcttcgg
     8221 tgggttagct gcgattatcc atatgtatat cgtcggacgc agaaaagact tcttcgaact
     8281 taaagatttt ttgatttcca gccgttattt cgaaagagaa aaccgatctg cctctgtctc
     8341 tatcattcgg gaaacactac cgtttatttt tgtcggctca gtcattacta tcgttcaaat
     8401 gatcgaccaa gtaacgatga aaccactgct tcactttttc cgtccagaga tcgcagatca
     8461 gcaactggaa ttcttattca gccgtgcttc tgtcaatcca aataaattga cattgatcct
     8521 gatctccatg gtaggaacag tagctataag cagcttgcca atattaagca cgatgaagaa
     8581 aaaagatcgg atacaaatcg aaaaaacagt aggagatagt ttttcgattg cgttattgat
     8641 tcttcttcct tcattgactg gaatgtcttt attagctgga ccactataca cattgttttt
     8701 tggctatgat cctgaaagtg tcggatattt ccaaatggct ttgctagctt ctttgttttt
     8761 ctctttattc acgatcctat ctacgatgct tcaatctttg aatcaccatc gttttgcgat
     8821 ccgattaact gtagaagcaa ttatcctgaa agtcatcttc caagtaatcg gattaggatt
     8881 agttggcgga tacgggatga gcttatcaaa tggcttagca ttcggtattg tctttgttcg
     8941 cggctatcac tttatgtgca aagaataccg aatagcacct ttagcaaaga tcagcaattt
     9001 tttcttaaaa actttccgct ccaccctgat tatgcttgca gtatgttttg ccgttttctt
     9061 tttgctgaat cagcgattgt caatggaatc aaaaagctat gcaatgattt attgtgtggc
     9121 tgtaggaagt attggcggct tagtattttt attttctcaa ttcggaaaaa atgggtttcg
     9181 aatgttgaaa aatgttaaac atcatagcag aagcaaagaa taggttaact aatcgttttt
     9241 tgctgtcact tgaaaaacgg caactcgtat gattacgctt gtcgtttttc tattttccaa
     9301 ttttagtttc atatctaatc ttacttatga tatagttata aggtctggaa aaattctttg
     9361 aaacgaggaa attaaacaca tgaaacttgc agtagttaca gatagttcgg catatctgcc
     9421 ggaacgtatc aagaaccatc ccgacttatt cgttattcca atccctttga ttatggatgg
     9481 gaaaatttat aacgaaggaa tcgatatcga agcggatgaa tattatggac ttttaaaaaa
     9541 cagtaaagat tttcctacta cttcacaacc tgtcgtaggg gaagttttgg gtttatatga
     9601 ggaattgaaa gagaaagggt atgacacgat tttaagtatc catctttctt cagggatttc
     9661 tggctttgtc aacacattat ttgcgatgaa agatgacatc agcggagtaa cggtcattcc
     9721 ttatgactct aagatcacta gtatgccgat gggacacatg gtagaagctg ccttagactt
     9781 aaatgatgaa ggtcagtccc ttgatgaaat acttgcacat atcgatcgaa tccgagataa
     9841 tacctatgct tatctgattg tagatgattt gaacaattta gtacgaggag gacgtttgac
     9901 aaatggtgca gcgctgattg gcagcttatt aaaaatcaaa ccgatcctga cattttctga
     9961 agggaaaatt gttttattcg aaaaaatccg ttcaagtaaa aaagcatttg ctcgagctga
    10021 agaaattatt ggtagaagaa ataacgaaat acaaccgcca gtcaaacttt acgtgataca
    10081 tgcaaataat ttagaagtag cgattgcgga gaaagagaaa ttgcagaaaa aatatccaaa
    10141 tgcagaaatc gaaattggtc atttcggacc agtcattggt acacatttag gtgaaaaagc
    10201 catcggcatt gccatttcag cgcaataatt ccttttattc aaaaaacata ccaaaaat
//
LOCUS       DAYYCY010000087        10192 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000087 DAYYCY010000000
VERSION     DAYYCY010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10192
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.087"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            119..625
                     /locus_tag="ACHAZB_001794"
     CDS             119..625
                     /locus_tag="ACHAZB_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFM8614440.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            642..1541
                     /locus_tag="ACHAZB_001795"
     CDS             642..1541
                     /locus_tag="ACHAZB_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HFM8614441.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            1628..2512
                     /locus_tag="ACHAZB_001796"
     CDS             1628..2512
                     /locus_tag="ACHAZB_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFM8614442.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(2704..2775)
                     /locus_tag="ACHAZB_001797"
     tRNA            complement(2704..2775)
                     /locus_tag="ACHAZB_001797"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2740..2742),aa:Arg,seq:ccg)
     gene            2835..4169
                     /gene="rpoN"
                     /locus_tag="ACHAZB_001798"
     CDS             2835..4169
                     /gene="rpoN"
                     /locus_tag="ACHAZB_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFM8614443.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            4318..5409
                     /locus_tag="ACHAZB_001799"
     CDS             4318..5409
                     /locus_tag="ACHAZB_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HFM8614444.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            5450..6451
                     /gene="gap"
                     /locus_tag="ACHAZB_001800"
     CDS             5450..6451
                     /gene="gap"
                     /locus_tag="ACHAZB_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFM8614445.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            6561..7751
                     /gene="pgk"
                     /locus_tag="ACHAZB_001801"
     CDS             6561..7751
                     /gene="pgk"
                     /locus_tag="ACHAZB_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFM8614446.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            7855..8610
                     /gene="tpiA"
                     /locus_tag="ACHAZB_001802"
     CDS             7855..8610
                     /gene="tpiA"
                     /locus_tag="ACHAZB_001802"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFM8614447.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            8726..10024
                     /gene="eno"
                     /locus_tag="ACHAZB_001803"
     CDS             8726..10024
                     /gene="eno"
                     /locus_tag="ACHAZB_001803"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFM8614448.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT         3565 a         1886 c         2112 g         2629 t
ORIGIN      
        1 tttgtcacac cttctttttt gtatataaaa atcccaagta aatacataaa aaataaaaaa
       61 attgaaactt ttagcgcacc ttgtacgtca tattaagtga ggcagaaagg agtggagaat
      121 gatttttact ggatataaga aaaaacggca gatcgaaaaa taccgtcacc tgctgaatgg
      181 actcatcgaa actgattatc aaaaaatgtt ccgtatcacg atgaattacg tacatgataa
      241 agaagaggcc ttagatgtga tgcaagatag ttttcataaa gccctgaact attttgaaaa
      301 agaaaaagag atcgagcatt ttagtgcatg gttttatcgg atattgatcc gcacggcatt
      361 agatagttgg cgcagacaaa aaaggacgcc tgtggactta tttgaaattc aagatatccc
      421 ccaacagtta cctttagaaa cttccgttgc agagctccat atgattttag ataaaataga
      481 cagtccggaa aaagagattc tgatcctcta cttttttgaa ggatttcgtt tgaaagaaat
      541 tgcgactatc ttggatatga atgaaaacac agttaaaacg aaaatgtacc gctcattgaa
      601 tgctttacgt caaatcctag aatgaaggag agaagagtgc catgtattcc caaaaagaaa
      661 atttaaaaaa actaaagaaa acttataaag aacaagcaat tcccaaaggg cttaaagagg
      721 agatccgcca gcgttttatt atcgaagagc aatcatttat aaaagcaaag cggagaaaga
      781 gacgtctcca agggttcgga gtatctttgg ctatcgtaat gattacaaca agtagccttc
      841 tttttaatag tcaagttcga agctttgcag aagatttacc catcttagga tcagtcattg
      901 aattgatctt aggcgaacgc tttactgatc gatctgaaaa aatcgatatt caggtaccga
      961 agatcaatac acaaaatgag aaagaaaata aaacaattca tggattaaat caaaaatact
     1021 ttcgcgaagg acaagcagat tttgaaaaag ctgaaaaaga atatggaaat tttgaaacgg
     1081 atcattacca agttttggga gattatcaga aaatcgtaga cgataaccgg tttctcgtga
     1141 ttgaaaggca gatcacccaa acagcagcag atagccatgt tgaaaaaaga tacgacacaa
     1201 tcgacaagaa aaacagtgtc cagctctcac ttccactttt attcaaagat gacacctatc
     1261 tatctgtttt gacaaaagaa gtcaaacggc agatggccga acaagtcaag gaagatccat
     1321 ctaagtacta ttggactgag caagatattc aagaagggac gatagaaaaa ccaacacttg
     1381 ttacgcctac acgaagtttt tatttgaaca aagagcatca attagttttg actttttctc
     1441 aatacgaaat tgctcctggt tatatgggta caccagaatt tgtcattcct aagtccgtga
     1501 caaaaacgat tttggcatcg gaagattatt tggatcatta gacgagaagt agttgcgact
     1561 agcagtttca attttcaagt aagtacaagt aaataataag aagagaaaaa gagaaagaag
     1621 agatatagtg aaaagaaatt atttagttat cagtattatt gctttatccg tgcttttgtt
     1681 tttcgaaatt acttcggtgg cctccttgat caccgaagta aaaggacaaa cacactcttc
     1741 ggagcattcg tcacaatctt tcaaacaatc agaacaatca accattaaaa gccaatcaac
     1801 tgaaacagct aacgtcaaag aagccattca agaatcagcg gaaaaggtac aaagtcagtc
     1861 atcacaatca acacaagaca gtacaaaaac gagccgagat ccggctgtca aaagcgtcgc
     1921 aatcagcttt gatgatggac caggagcgac cacaacaccg cagcttctgc gaatcttaaa
     1981 agaaaaaaac gtgcatgcca cttttttcgt cttaggagaa aatacggcgc agcatcctga
     2041 aatcgtgaag cagacagctg aagcagggca cgagattggc aatcacacat acgatcatca
     2101 gaatctggcc actctttctg cccagtcgat gacggaggaa gtgacgaaag cagacactga
     2161 gatcaaaaaa gtcactggca aaacccccac ttttgttcgt ccgccctatg gttctgtcac
     2221 gaatgtagga gcaacgatca tccagcgacc gatcatcgaa tggtctgttg attcagaaga
     2281 ttggaagaca agaaatcctg atttgatttt gcagaagatc caagcaacag tatacgatgg
     2341 ggcaattatt ttattccatg atatctatcc tgaaacgatt cgtgccgttc ctcaagtgat
     2401 cgattatctg aaagagcaag ggtatcgcat cacgacagtt ggggatttat tagggcatcc
     2461 aacggctgtc gagaactatt atggcagaaa cgatcatcgg ccagttcagt aagtgagaca
     2521 agtatcttta ataaataaca cattttaata agtagttgaa cacataacta ccttacttgc
     2581 aaaggcttaa caatatctat acaggtacaa caaaaaactg tccaccaatt ttctttggcg
     2641 gacagttttc acttaaaaat aaagaagcaa ctattcgtaa attatgctaa aatagcagta
     2701 gaacgcgtcc tgagggaatc gaacccccgt ctcaagaacc ggaatcttac gtgatatcca
     2761 ctacactaag gacgcaagca ctgggataat tgtaactaaa cagataaaaa attacaaggg
     2821 gattggacaa aaaaatgaaa tttgagcaaa atttttcaca aaaacagcag cagacacaga
     2881 aactagccat gacgcaacag ttgcaacagt ccatccaagt tttacaatat aacagtgagg
     2941 agctgttggc attcgttgag aatcaagcaa tggaaaatcc attggtggaa gttgttgagc
     3001 cggaatggca gccagattac ataaaagctt cttcatcttc ttatgaagga gaagagacaa
     3061 attacctaaa tcagattcct gatacaaaag ggtctttatt tgattcattg atcgaacaag
     3121 tccatttgaa ttaccgagat acttttttga ggaaaatcgt tctttatctt gtagaataca
     3181 tcgatttgaa cggatttttg acgattagtt tagaagaagc cggaaaagag attggagcta
     3241 cgcctatcca aatgctcgat gcgttgactc tgattcagca attagaacca gctggagtag
     3301 gtgcacgtag tttgcaagaa tgtctcatgc ttcaaacaga acgagatgat tatgcgccgg
     3361 aattagctta tatcgtgttg gaagaatgtt ttgaggaact ggtggagcga aaatggaaag
     3421 aaatcgcaca acgtttttct gttgatctac atgctgttca gcagatattc gactatttac
     3481 aaacgttgag tccaagccct ggccggattt tcgaccgtag cagcgaattg tacattattc
     3541 ctgatgtccg ggtattagtg gacgacgata aaaatgtaca agttatctca aaccgaaaaa
     3601 atcagccaaa tatacgcttt caagagagtt acttcaaaca aatgtcacaa caagcagata
     3661 aagaaactga aaattattta aaagaacgga aacaggaatt cgaatggttg aagaaaacga
     3721 tcctacaacg tggggataca attttgcggg ttgctcaagt cattgtttcc cggcaaaaag
     3781 ctttctttat cgacaaggaa cgtccaatca agcctcttac gttaaaggaa gttgcaactg
     3841 agatagatgt gcatgaatcc acggtcagtc gtgcagtcaa cgggaaatat ttggaaacag
     3901 acttcggtgt atttgaatta aaaaaatttt tcactacacg aattcctacg aatagtacag
     3961 aacagacaga agacctttca gcagatacag cgaagaaaaa gctgcaggaa ttagtcgatc
     4021 aagaagacaa aaacaagccg ttgtctgacc agaaattggt tgaattgctg aaaaaagatg
     4081 agatagcgat ctcaagacga acagtagcaa aatatcgtga tttattagga atcccgagtt
     4141 catcaaaaag aaaacgttat gataactaat cagtcaaaag actagaaaaa gtatttcact
     4201 ttttctagtc tttttagttg aaaaacgaga gcaaaactgt tagacttaca gtcgtgaggt
     4261 tgagatttcc ctttattgat gtgaaattcg aatagttaat ttcttttttt atcgtatatg
     4321 ggtcgtttta agtctacatt ggacgtaaag tgtccaacta aggagatcgc tatgctggat
     4381 gaaataaaaa tgattgaatc tgttgcacca gatattatcg aagtgttgca agaacgctat
     4441 aaaattttac gaaatatcta ttggatgcag ccgattggac gtcgaagttt atcggaaagt
     4501 atggggatca cagaacgagt attgcgaaca gaaacagatc ttttgaaaaa tctgaatttg
     4561 atcgacacct caaaaagcgg tatgacgctg acaactaagg gcgaagaagt ttatcaaagc
     4621 cttgaaaact tcatggatca gcttttaggt acgcatcaaa ccgaacaaca gttggcagaa
     4681 tattttggta accaacgctg tatcgttgtt tcaggaaaca gcgaagagca gaccaaagtg
     4741 gctgatgcat tcggcgaagc gctgagcgaa gccctagacc gactacttcc agaaggcgag
     4801 aatatcatcg ctgtgatggg aggaactact atggctgtgg tagccgaaca attgtctaat
     4861 ctggaaaata agaaacggca taacctgttt gtccctgcaa gaggcgggat tggtgaagcg
     4921 ataactgtac aggcaaattc tgtcagtgca agaatggctg ctaaagcaaa cggtaaccac
     4981 cgtgcacttt atgttccgga acaattaagt ctggcaactt acaattcttt actcaatgag
     5041 ccatctatcc aagaagtctt aaacttgatt agtgaagcga attgtgttat tcatagtatt
     5101 gggcgtgcat tacacatggc agcacgtcgt aagatgactg aaaaagaatt agtcatgctg
     5161 aaacaagcaa atgctgttgc ggaatctttt ggctactttt tcaatgaaaa aggtgaagtc
     5221 gtttataagg tccctagaat cggcatagaa ttgcaagatt tggaaaaagt cccgatcatc
     5281 atggcaatcg cgggcggaaa atcaaaagcc aaagcaattc gagcttatat gaaaaatgca
     5341 ccaaaacaaa cgtggctcat cactgatgaa gctgctgcaa atgagatttt aaaaggggta
     5401 accctttaaa ataaatattt ttttgatttt cataaggagg aaatcttaaa tgacagttaa
     5461 agtaggtatt aatggttttg gacgtattgg acgtctagca ttccgtcgta tccaagatgt
     5521 agatggaatc gaagtagtag caatcaacga cttgacagat gctaaaatgt tggcacactt
     5581 gttaaaatac gacacaactc aaggacgttt caacggaaca gttgaagttc atgaaggttc
     5641 attcaacgta aacggaaaag aagttaaagt attagctaac cgcaacccag aagaattacc
     5701 atggggcgaa ctaggcgtag atatcgttct agaatgtact ggtttcttca cttctaaatc
     5761 agcagctgaa aaacatttaa cagctggtgc taaacgcgtt gttatctctg ctcctggcgg
     5821 aaacgatgtt ccaacaatcg tttataacac aaaccacgaa acattaactg gtaaagaaac
     5881 agttatctca ggtgcttcat gtactactaa ctgtttagct cctatggcta aaacattgaa
     5941 cgacaaattt ggtgttgttg aaggattaat gacaactatc cacgcttaca caggtgacca
     6001 aatgactcta gacggacctc atcctaaagg tgacttccgc cgtgcacgcg ctgctgctgc
     6061 aaacatcgtt cctaactcaa caggtgctgc taaagctatc ggtttagtaa ttcctgaatt
     6121 gaacggtaaa ttagacggag ctgctcaacg tgttcctgta ccaacaggtt cattaacaga
     6181 attagtaaca gttcttgaaa aagaagtaac tgttgacgaa atcaatgcag caatgaaaga
     6241 agcttcaaac gaatcttacg gatacaacac agacgaaatc gtttcttcag atatcgttgg
     6301 tatgacttac ggttcattat ttgatgctac acaaactaaa gtaatgacag ttggcgacaa
     6361 acaattagtt aaaactgttg cttggtacga caacgaaatg tcatacactg cacaattagt
     6421 acgtacttta gaatacttcg ctaacttata agatagacaa acgtacaagt gtaaattata
     6481 gacatgtaca tttaaaagcg gggaagcaat cgcgcttctt ccgctttttt tgataataag
     6541 tttattcagg aggaaataaa atggctaaga aaacagtgaa agatatcgat ttaaaagaca
     6601 aaaaagttct tgtccgtgtt gacttcaacg ttcctttgaa agacggcgtg atcactgacg
     6661 acactcgtat caaagcagca ttgccaacaa tcaactacgt tctagaacaa ggcggaaaag
     6721 caatcctttt ctctcacctt ggacgtgtaa aaacagaaga agataaagaa ggaaaatctt
     6781 tagctccagt tgcaaaacgt ctaggcgaat tattaggtaa agaagtaaca ttcgtacctg
     6841 aaactcgcgg agaacaatta gaagaagcaa tccgcaacat gaatgacggc gacgttgtcg
     6901 tattcgaaaa tacacgattt gaagatattg acggtaaaaa agaaagcaaa aatgatacag
     6961 aactaggtaa atactgggct tcattaggcg atgtatttgt taacgatgct ttcggtacag
     7021 ctcaccgtgc acatgcttct aacgtaggta tcgcttcaac tggtatccca acagttgctg
     7081 gattcttgat ggaaaaagaa atcaagttca tcggtgaagc agtagaagaa ccaaaacgac
     7141 caatgattgc aatccttggt ggcgcaaaag tttctgataa aatcggcgta atcgaaaact
     7201 tgattcctaa agcagataaa atcttgatcg gtggcggaat gacttacaca ttctacgaag
     7261 caaaaggaat caagatcggt aactctctag tagaagctga taaagtggaa ctagcaaaag
     7321 aattaatcga aaaagcaggc gacaaacttg tcttgccaat cgacaatgtc tgtgcaccag
     7381 aattttcaaa tgatgttgaa acacaagtaa tcgaaggaga cattcctgat ggtttgatgg
     7441 ctttagacat cggcccagca tcagtaaaat tatttgctga tacattacaa ggtgcaaaaa
     7501 cagttgtttg gaacggacct atgggtgtat ttgaaatgag taactttgct aaaggtacaa
     7561 tcggtgtttg tgaagcaatc gctaacttag aaggcgcaac aacaatcatc ggtggcggag
     7621 actcagccgc agcagcagaa caattaggat ttgctgacaa attcacacac atctcaacag
     7681 gtggcggcgc aagcttggaa ttgcttgaag gtaaagaatt accaggacta gcagcaatca
     7741 acgataaata aacgaccaag tttgctcaat tctgacgagt aatcgatcaa aaggtgaagg
     7801 gagttttcat gactccctag ccatttttca ataaattaag gagtgtgttc ctcaatgcgt
     7861 aaacctatta tcgcaggtaa ctggaaaatg aacaaaactg catcagaagc aaaagaattt
     7921 gctgaagcag taaaaaacaa aatcccttca aatgacaaag ttgattcagt catcggatct
     7981 cctgcattat tcttacaaga attaatgaca tcagctgaag gtacagaact aaaaatctca
     8041 gcacaaaact gctactggga aaactcaggt gcgttcactg gtgaaacttc accagcagct
     8101 cttgctgatc taggtgttca ttatgtgatc atcggacatt cagaacgtcg tgaatatttc
     8161 catgaaacag acgaagacat caataaaaaa gcgaaagcta tctttgcaaa tggtatgaca
     8221 ccaatcctat gctgtggcga atctttagaa acctatgaag caggcaaaac tgctgaatgg
     8281 atcgaaggac aaattacagc tggtttagct gatctttcag ctgaacaagt gtcaaacatg
     8341 gttattgctt atgaaccaat ctgggcaatc ggtactggta aatcagctga cgcacaaatc
     8401 gctgacgaaa tttgtggagt tgtacgtcaa acagttgaaa aactttatgg aaaagaagta
     8461 tcagaagcag ttcgtatcca atacggcggt tctgtaaaac cagaaaatat tgctgaatac
     8521 atggcaaaag aaaatgttga cggtgcttta gtcggtggcg caagcttgca agcagattca
     8581 ttcttgtcat tgttagacgc tgtaaaataa ttgttgaaaa tttgcgaaaa atgcaagaaa
     8641 tgtttgcact aatgagcaaa attaactaaa atcgtatatg agggaaatcc cttaggaaaa
     8701 aacaaactca aaggagagac aaaacatgtc aattattact gatgtttacg ctcgcgagat
     8761 cttagactca cgtggtaacc caacaatcga agtagaagta tacacagaaa gcggagcttt
     8821 tggccgcgga atggttcctt caggtgcttc aactggtgaa tacgaagcag ttgaattacg
     8881 tgacggagac aaagctcgtt atggcggtaa aggtgtaact aaagctgtcg acaacgtaaa
     8941 caacatcatt gctgaagcaa tcattggcta cgatgttcgt gaccaaatgg ctatcgacaa
     9001 agctatgatc gctttagacg gaactcctaa caaaggtaaa ttaggtgcga acgctatttt
     9061 aggtgtttct atcgctgttg cacgtgcagc tgctgactac ctagaagtac ctttatacca
     9121 ctacctaggc ggattcaaca caaaagtatt gccaacacca atgatgaaca tcatcaacgg
     9181 cggatcacat gctgataact caatcgactt ccaagaattc atgatcatgc ctgttggcgc
     9241 tccaacattc aaagaagctc tacgtatggg tgctgaagta ttccacgcat tagcttcaat
     9301 cttgaaagct cgcggattag caacttctgt tggtgacgaa ggtggatttg ctccaaacct
     9361 tggttcaaac gaagaaggtt ttgaagtaat catcgaagca atcgaaaaag ctggctatgt
     9421 acctggtaaa gacgttgttc ttgctatgga tgctgcttct tcagaattct acgacaaaga
     9481 aaaaggtgtt tacgtactag ctgattcagg cgaaggcgaa aaaacaactg acgaaatgat
     9541 caaattctac gaagaattag tttctaaata tccaatcatc tcaatcgaag acggattaga
     9601 cgaaaacgac tgggacggat tcaagaaatt aactgacgta ttaggcgaca aagttcaatt
     9661 agttggtgac gacttgttcg taacaaacac tcaaaaatta tctgaaggta tcgaaaaagg
     9721 aattgctaac tcaatcctta tcaaagtgaa ccaaatcggt acattaacag aaactttcga
     9781 agctatcgaa atggctaaag aagctggcta cacagcagtt gtatctcacc gttctggtga
     9841 aacagaagat tcaacaatct ctgatatcgc tgttgcaaca aacgctggcc aaatcaaaac
     9901 tggttctcta tcacgtactg accgtattgc taaatacaac caattactac gtatcgaaga
     9961 ccaacttggt gaagttgcag aatacaaagg tttgaaatct ttctacaact taaaaaacaa
    10021 ataattcttt tctaatgaag atacatgatc gactattgaa agatagtcga gtatgatcac
    10081 ttagttaagt aatcgaactc tctacttgct gaaatatcgg ctctttgtca ataaggactg
    10141 atgagttgtg caaaatcaaa tctgagtcag aatgaaccac attctggctc ag
//
LOCUS       DAYYCY010000088        10164 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000088 DAYYCY010000000
VERSION     DAYYCY010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10164
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.088"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            29..1204
                     /gene="mltG"
                     /locus_tag="ACHAZB_001804"
     CDS             29..1204
                     /gene="mltG"
                     /locus_tag="ACHAZB_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFM8614449.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            1340..1819
                     /gene="greA"
                     /locus_tag="ACHAZB_001805"
     CDS             1340..1819
                     /gene="greA"
                     /locus_tag="ACHAZB_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFM8614450.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            1988..2719
                     /gene="liaF"
                     /locus_tag="ACHAZB_001806"
     CDS             1988..2719
                     /gene="liaF"
                     /locus_tag="ACHAZB_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein
                     LiaF"
                     /protein_id="HFM8614451.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            2716..3783
                     /gene="liaS"
                     /locus_tag="ACHAZB_001807"
     CDS             2716..3783
                     /gene="liaS"
                     /locus_tag="ACHAZB_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     LiaS"
                     /protein_id="HFM8614452.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            3790..4422
                     /locus_tag="ACHAZB_001808"
     CDS             3790..4422
                     /locus_tag="ACHAZB_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM8614453.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            4562..5227
                     /locus_tag="ACHAZB_001809"
     CDS             4562..5227
                     /locus_tag="ACHAZB_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HFM8614454.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            5255..5548
                     /locus_tag="ACHAZB_001810"
     CDS             5255..5548
                     /locus_tag="ACHAZB_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/YadR/YfhF family protein"
                     /protein_id="HFM8614455.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(5616..6563)
                     /locus_tag="ACHAZB_001811"
     CDS             complement(5616..6563)
                     /locus_tag="ACHAZB_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFM8614456.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(6741..7544)
                     /locus_tag="ACHAZB_001812"
     CDS             complement(6741..7544)
                     /locus_tag="ACHAZB_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HFM8614457.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            7690..8604
                     /locus_tag="ACHAZB_001813"
     CDS             7690..8604
                     /locus_tag="ACHAZB_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFM8614458.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            8652..9122
                     /locus_tag="ACHAZB_001814"
     CDS             8652..9122
                     /locus_tag="ACHAZB_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HFM8614459.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            9207..9815
                     /locus_tag="ACHAZB_001815"
     CDS             9207..9815
                     /locus_tag="ACHAZB_001815"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HFM8614460.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            9949..10149
                     /locus_tag="ACHAZB_001816"
     CDS             9949..10149
                     /locus_tag="ACHAZB_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8614461.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
BASE COUNT         3462 a         1564 c         2095 g         3043 t
ORIGIN      
        1 acgaacttga tagtaaggag gagttgtttt gtcaggaaac aaacaacacc caagtaaaga
       61 taaaaaagaa gccagtaagg cagctacagc agatataatc gacaagcgga gaaatctgcg
      121 taaaaaagaa gataagattg tgaggaagat cattttagtg attgctctta cattattgat
      181 tattggcgga tttcttggat ttaccgtgta tcgttatgta gatagcgggt tgaagccttt
      241 agataaatca gatgaccaat tagtccaagt cgagatcccg agtggctctt ctaacaagca
      301 gattggtgag attttggaga aggacaatat tattaaaagc gggattgttt tcaattatta
      361 tacaaaattt aaaaatttaa ctggctttca agctggctat tatcagttag cgcctaatat
      421 gactcttgac gagattggca aacagttgca agaaggcggt acttctgagc caacaaaagt
      481 tgcagatggg aaaattgcca ttccagaagg gtacgatatc gatcagattg ctgaacgggt
      541 agcgaaggta actggtaaag acaagaaaga atttttagat ttagtgaatg atgagacttt
      601 ctttaacgag ttgcaccaaa aattcccaga actgttggaa agtgcatcta aagctgagaa
      661 cacaaaatac cgtttggaag gttacttatt cccagccaca tatgattatt acacgagtac
      721 ttcgcttaag gatctagtga ttgaaatggt gaacaagacg aatacggtca tgcaaaacta
      781 ttattcagct atcaagcaga aaaatttgac tgtacaagaa gttttaactc tagcttctct
      841 agttgaaaaa gaaggcgtaa aagaaaatga tcgtaaaaac attgcacaag tcttctttaa
      901 ccgaatcaaa gcaaatatgc cacttcagtc agatatctct gttttatacg cattaggtga
      961 acacaaagaa ttagtgacgt atgaagatac agctgtagat tcaccatata atttgtatac
     1021 caataccggc tatggacctg gaccttttga caatccaagc gaagaggcaa tcaaggcagt
     1081 attagaacca gcagaaaatg actattatta tttcgtagca gatacgagca ctggaaatgt
     1141 ttatttcgct aaaacttatg aagaacatat ggaattagtg gaaaaatatg taaataattc
     1201 ttagaaaaac aggctctaga cattgatcat ttttagaaaa acatttacat ttgaagaaaa
     1261 gcgtggtaat cttttttgga ctatattcct caaaagatta ccttttgcgg tattttatcg
     1321 aaaaaaggag acgttacaca tggtagagaa agtttatcca atgactctag aaggtaaaga
     1381 aaaattagaa aaagaattag aagaactaaa aacagtcaaa cgcggagaaa tcattgaacg
     1441 aatcaaaatt gcccgcggtt tcggagatct ttccgaaaac tctgaatatg aatcagcgaa
     1501 agatgaacaa gcatttgttg aaggaagaat cacaacaatt gaaaatatga ttcgttttgc
     1561 tcaaatcatc gataacgacg gtgtagatgc agatgaggta tcaattggta aaacagttac
     1621 attcatcgaa ctaccagatg gagaagaaga agagtacacg attgtcggaa gcgctgaagc
     1681 agatccgttt tctggcaaaa tttccaatga ttctcctatt gcgcaagcat tgattggcaa
     1741 acgtaaaaat gatgaagtaa ctattgctac tccaggtggc gatatgttag ttaaaatcgt
     1801 caaagtagaa caagcgtagt ctgactgaaa gatctagaga gttcatctta ctgatgatac
     1861 actctagatc tttttctctt tgtgtatttt agatttcaac aaacgatctt acatctaaag
     1921 tcctatagcc aacagttaaa aaaataggta tgatgaaatg agaatgtgtc aggagggaga
     1981 aagagcgatg aatagttctt ggcgtttttt tgtggtagtt gaagcactac tgctgttatt
     2041 tgctgtttgg caaatcgtaa acaatactgg acttttgctt ttagtgatat tcggtatttt
     2101 caatatatat ctagctatgc gcaaacatcc tcgtactaaa tttcaaaatt ttcaattgat
     2161 attaggaagt ttggtcattt tttttagctt agtcaacagt cctgctttat ggatgatggc
     2221 tgtatttgcc gttttattta ttggtctgaa aggtgtagag atttcaggga tagatttaac
     2281 caaaaatgct ttttggcgaa agaaacagat catgatgatc caaacagagc aattaaaaac
     2341 acataataat gagcggaaaa gacagcaatt attcggtaac caacgtattg gaaacgacgt
     2401 ctatgaatgg gacgatatca acatcgccgt catttctggt gatactatca tagatttggg
     2461 caatacgctt ttgccaaaag atgataatat tgtgattgta agaaaaggga taggcagaac
     2521 acggatctta gtacctttgg gcgtagccat ccgattagag catgcgacat tagtaggaaa
     2581 tgtcttgttt gaagaagagc aattttcttt gaaaaatgaa caaatcaaaa tctacagcag
     2641 tgattatgat gaaaaccctc gtagattgaa gattattact aataccttga ttggagatgt
     2701 cgaggtgata cgaatatgat gggaaaaata tccagagcga tgctagctgt ttattcgggg
     2761 attgcggctt tccttattat tctattttca cttttcactt atttttatgc cagcaatcaa
     2821 agtcattggt ggggagaatt actgcgtgca cgtttattat atgttccgct tatcttccat
     2881 ttgttagcca tttccttagg tgtaggatta attgtctttc tattattatc acttattcaa
     2941 aagacaaaat acgggaaaat cgaagaaaag ctgcgtgcac tttcttctgg caattatgaa
     3001 tccaaattgt tgcttcttcc gatcccaagt gcatcggacg atttatacat caaagatatt
     3061 gataaggaaa tcactaagat aaaagaaaaa atgattgaag tatctagtga attacaaatt
     3121 gtaacgagcc gtccccaata tgtagatgga caaaccaaag aagaaatttt agaattagaa
     3181 agacatcgat tagcccgtga gctgcatgat tcagtttcgc aacaattgtt tgcagcaatg
     3241 atgatgatgt cggcattgac ggagcaagca gaaaaaagcg agacaccaga aatgtttcgc
     3301 aagcagttga aaatggtagc agagatcatc aacgcttccc agtctgagat gcgtgcgctg
     3361 cttcttcatc tacgtccagt caatttagaa gagaaaagtt taaagcaagg tatcgagcag
     3421 ttattgaagg aattgcaaaa taaaatccag atttcgctga aatgggatgt agaagatgta
     3481 aaactatcta gttccattga ggatcacttg ttccgaatcg ttcaagaatt gttatcaaat
     3541 acattaagac atgctaaagc caatgaatta gaggtatatt tgcacaaaat agacaataat
     3601 cttttgttac gtattattga cgacggaaca gggtttaata tgaatgaaac gaaaacggga
     3661 agttatggat tgaacaatat caaagaacga gtagctggta ttggtggaac agtaaaaatc
     3721 attagtttta aaggtcaagg tacaagtgta gaaatcaagg tccctttgat gaaggaggca
     3781 tagataagga tgataaaagt tttattagta gatgaccatg aaatggtgcg cttaggcgtc
     3841 tcttcttatt tatctattca agaagacatt gaagtaattg gagaagctga aaatggacga
     3901 caaggctatg agaaggcgat gacacttcga ccggacgtca ttttaatgga cttagtcatg
     3961 gaagaaatgg atggtatcga atcaacaaaa gcaatcttaa aagattggcc agaagctaaa
     4021 atcattatcg taaccagttt tattgatgac gaaaaagttt atcctgccat tgaagcaggt
     4081 gcagcagggt atttgttgaa gacctctacc gcacatgaaa ttgctgatgc tataagggca
     4141 acgcaacgtg gcgagcgagt attagaacca gaagtgacaa caaaaatgat ggaaaaaatg
     4201 agtcggcgaa atgagcctgt attacatgaa gaactaacga atcgggaaaa tgaaatttta
     4261 atgctgattt ctgaaggaaa aagtaatcag gaaatagcgg acgaattgtt tattacactg
     4321 aaaacagtaa aaacacatgt ttccaatata ttggctaagc tagaagtgga agaccgtaca
     4381 caagccgcta tctatgcatt caaacatggt ttggtgaaat aaaaacgatg ataaagagga
     4441 taatcaaaag ctgagaaaat aactattatt tttcttggct tttttacaat aatgatgctt
     4501 gcttttgttt ttgtaaaggc tttgttatac tgagaaaaga agatactaaa ggaggaaaac
     4561 ggtgaaacaa aattttgcaa ttgtaggtct tggacgattt ggcggaagta tctgccgaac
     4621 actgatcgaa gctggtcaag aagtactggc tattgatagt aatgaagatc gggtcaatga
     4681 atatatgaat atagccactc atgctgttgt ggcaaatgca caggatgaaa tgacacttcg
     4741 atcattaggc ataagaaatt tcgatcatgt aattgtcgct attggagaag atattcaagc
     4801 cagtatctta gttactctga tggtaaagga aatgggcgta ccaaatattt tagcaaaagc
     4861 ccaaaatgag tatcatgcca gagtacttga aaagatcgga gctgatcgcg tcgtccatcc
     4921 cgagcgagac atgggaataa ggattgcgca taatttagtg tcaaagaata ttttagatta
     4981 cctagagttg tcagataaat attctttagc agaaatccga gtctccaatc ctaaattttt
     5041 caacaaaaca ttagctgaat taaatttcag gcaacgtttt gacttaacag tagtcgccat
     5101 acgaagagta gatcaaacgg ttatcgcttc tcctgcagca gatgaatatg ttcgtgaaaa
     5161 tgataatcta ctggttattg gagagacagg cgatgtggat attctagacg ataagatgaa
     5221 tcaatagaaa gaaaagagaa agtaggttgt tgggatgaaa ttaacggtaa cgccaaaagc
     5281 acaaaagtgg tttgactcag aaattgatct gccagaaact tacggaatac gtttttttgg
     5341 aaaagtctac ggcaaaacag aggttcatga tggtttttcg attggaatgt cagttgaaca
     5401 gccagaacat cctgtaaaga aagtggaaat ggatgggaca ctttttttta tcgaggaagc
     5461 agatgaatgg ttctttaaag gatatgattt acttgttgat tatgatccca acctggagga
     5521 gccaacctat cactttaaaa aacaataaaa aaagagaggg tgacacaatt tttgtcacct
     5581 tctctttttt cttttgaaaa tattgttttc agctattaat atcctgcaga caattttact
     5641 tgattggttt gaagctcatg ggtggcaaag tagcttttca gattggttaa aaaaatagcc
     5701 attaatttct tttgaaaatc aggtgtcaaa cctgcaatgt gggaagtaat cagtacattg
     5761 tccattttcc aaagaggatg atcttgtgga agaggctctt cctcaaaaac gtctaatgct
     5821 gcaaaggaaa gttgctggtt atttaaagcc tgcaccagat cttgtgtctt aacagaagca
     5881 ccacggccaa cattgatgaa tacagatttt ggatcaaagt gttcaaacat atgctgatca
     5941 aagagcccag ttgtttcctc cgttagaggt aagatattga ctacgaaatc tacttcagga
     6001 agaacttcct ttagttgatt taaagaaaaa gtcttttcca ttccttctgc tggatgaccg
     6061 gatgtattga ttccatagca gttcatgtct aatgctttgc atttttttgc aatcattttt
     6121 ccaatatggc cagttccaat gattagtatt tttttccctt ggatagattg gtaatggacc
     6181 gatgaaccta accatttcat gtcactctgt gccttttgtg cttgaaaaag accacgagaa
     6241 tacccaagga tgattcctaa gacatgctcc gtaatcgatt cactgtggat gccactacca
     6301 tttgaaagta aaatatgttt ctttgaaaaa gtctcaaggg gtagctgatc aacaccagct
     6361 gagatagatt ggacccattt caaggtagaa gaggctaata gtttttcttg gtagtctgag
     6421 ttccagccca aactgatctc tacattttgt agatcctctg gttgtaattc tgttttaaag
     6481 atataaccag gcgctatagt atggatttct tcgataaatt catcacgaaa tggacgatct
     6541 gcataaatga ttctttctga catagttaat ttcccgcctt tctttactct aagtgtagcg
     6601 catacaagaa gaactttgaa gcgccttgct tgttttttga gaaagaaata tttttcaaat
     6661 aaaaaagggg ctgtgacaaa aaaatgtcac aacctctaaa agggtctatt gaagtagaaa
     6721 taacgagttg tctttatttc ttaattgatc gatatttttg cccatccttt gacagcttga
     6781 gcaaaaataa agattcctgc aaaagcaatg aaggcgctag tgtcaaaaga aggaagaaag
     6841 accatcaaga ttgttgcaat tatagtaggc aatgaaattg aatagatcaa tgtttttaaa
     6901 caatcaagaa aagtagcttt tgttagtcga aagcgtgcat agagaaaagc agcaatgttg
     6961 gtaaatagca aagtgatcag cagattcaga aaactaggat aaatagaaaa tataaatgtc
     7021 aggactttta tccaaagtgg tatagatgct tcacttaaaa aagaacggag ctgcttacct
     7081 gttaagtttt ttaaagattc attggtgtag ggaagttcta attggttatt gttcaacaaa
     7141 gcagaagtcg tgcctgtatt aggaagagct atgataagtt tgttttttaa catcccgaca
     7201 ctaacaaaat tccccatcaa atcagtagag atgtcctgtt ctgttcgttt tccctctgga
     7261 tcaaatgtga agataatgga gtttgtttgg taaatgaatc cttctttttc ttcggtttga
     7321 agctgcccgt tttggattgt aaaatcagga atgcgagtag caatcttttg cccatccaat
     7381 tgaatgttgt ttaagacttg aaaaacttga aaagagattg gcaaagccat aattatactc
     7441 aaaactaaaa gatagacaat ggctttccaa aatgggacat ttctcgcctc ctttaggtct
     7501 ttgaatcgta taaaggaact gataattaat tgttttgttg tcataagtac tcctcatcta
     7561 gtagtgtcat cacttgcaat tctagcgaag tatcaagtga aatacaagtg ctttaaggaa
     7621 acagctgaat gattggtgaa atatcaaaaa aatgttattc ttgtctaaag actatccaat
     7681 ataaataaaa tggaggaagg aatagtgcct acacagccac agagatcatc aaaaaaacta
     7741 tttggagtag tggttttgaa tgcggtgatc actttatcag aatttattgg agggattatg
     7801 tcagggagtt tgtctcttgt ttcagatgct ttccataacc ttagtgatac attggcaatt
     7861 attttttctt acggggcaca aaaaatggcc cgaaaagaag caaataaaaa acgtacgtat
     7921 ggctaccagc gtttagaaat attgagtgct ttcatcaact cattcatatt gatcatatta
     7981 tccttgtttt taaccgttga agcattcaag cggttcactt caccagaaaa gataaatagt
     8041 catttgatgc taacagtagc agttattgga ttgctcgcga atttgttctc agtattatta
     8101 ttacgacaag aagcagatga aagtttgaat ataaaatcta gttatttgca tttgttaagt
     8161 gatacactgt cttcggtaag tgtgattatc ggagcaattt tgatccgttt tttcgatatc
     8221 tactggatcg atccagttat tacactgatc atttcgatat atatattgat agaagcgata
     8281 atggtgatca aaaaagctgc cgctattttg atacaaagtg cacctgctat cgactatgaa
     8341 aaaatggaac aagaaattga agcaatcgat ggggtaaagg atgttcatca tgtccatatt
     8401 tggcagtata gcgaaaagat aattattttc gacggacata ttgattttga agatcagcta
     8461 ctaagcgaga ttgaaaagat atatcctcgt ataatttctt tattaaaatt aaaatatggt
     8521 attacacatg tgacgatcca agcagagaca aaggcaaagg atcaaaaaaa attaattttt
     8581 ttaaataaag atgaaagtta ttgaaatata gagtatgata aataagaatt tttaataagg
     8641 taggttggct catgaagcag tatcgacagt taaaaggata tttagaagca aaaggatatt
     8701 gggagatact agtctatctc ttttttggtg gtttggctac gattgtaaat tttgttagtt
     8761 ttgctctcgc acgccaatat tttgatttaa gtatggcctt atccaattct atttcgtggt
     8821 tttgttctgt attgtttgct tttgtaacga acaaactttg ggtattccat tcaaaatcac
     8881 cgaattttac acatgcactt attgaatgcg gtaagttctt cttctaccgt atcctttctt
     8941 acggattgga catgggggca atggtattgt tgatcaatgt tatgaacagc aatgagtatg
     9001 tggcgaaaat catcactcaa attattgtta tccttgcgaa ctatattttc agcaagttgt
     9061 ttatttttaa agaaactgag gtttttgaag aagaagtagg tcaaagtgaa aaagacgact
     9121 aggcataaca attgaagaat ttccttctct ttgttactct ttgtaatgta gacattatta
     9181 agaaaacatt gaggaggaat ttttttatga cttacacatt accagatcta ccttatgcat
     9241 atgacgcatt ggaaccttat attgacgaag aaacgatgca tctgcatcat gataagcatc
     9301 acaatactta tgtgacgaat ttaaattcag caattgagaa atacccagaa ttaggcgaaa
     9361 aaacaataga agaattatta tctgatatgg acgctattcc aacagatatc aagacagctg
     9421 tacgtaacaa tggtggcgga catgctaacc attcattttt ctgggaaatc atggcaccaa
     9481 atgctggtgg cgaacctaca ggagaaataa aagaagcgat taatgaagct tttggtgatt
     9541 tttcttcttt taaagaagaa ttcaaaaaag cagccgctgg acgatttggt tctggatggg
     9601 cttggcttgt aatggaaaat ggaaaattag ctattacctc tactgcaaat caagattctc
     9661 cattgatgga aggaaagaca ccaattctag gtttggacgt ttgggaacat gcttattatt
     9721 tgaaatataa aaatgtccga ccagattata ttgcagcatt ctggaatgtt attaattggg
     9781 atgaagtaaa caaacgtttc gaagcagcaa aataaccatt tcctccgtca tatcagtctt
     9841 aaaaataatt tggaaaataa aagagcgaaa gcttgctagc gttttctaaa ttgggtataa
     9901 tgttcttgca aggttacttg caagaacttt tttggaggat atacatttat ggaacacgga
     9961 acggttaaat ggttcaacaa tgaaaaagga tttgggttta ttacagtaga cggcggagat
    10021 gacgtatttg ttcatttctc agcaattcaa ggagacggct tcaagagcct agaagaaggt
    10081 caagaagtag aattcacgat tgttgaaggt gcacgcggac ctcaagctgc tgaagtgact
    10141 aaattataat aatttgttga ataa
//
LOCUS       DAYYCY010000089        10111 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000089 DAYYCY010000000
VERSION     DAYYCY010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10111
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.089"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..2668
                     /locus_tag="ACHAZB_001817"
     CDS             <1..2668
                     /locus_tag="ACHAZB_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM8614462.1"
                     /translation="QPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTGNMSSVTAEKI
                     SKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQINEAFHSVVG
                     HDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIHEQIIEKDAPF
                     AILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFLDEVHRLSKEN
                     QEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRRIQISVMLPTF
                     EDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGKLKNIIKISCA
                     DVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTDRIKPFDYDSI
                     LEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMFRKVWVSVLGK
                     TYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQFLEHLPPLSE
                     KDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCGNYVFEALDMP
                     VESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGELLLLNNLTTS
                     IALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMSGLGISDKLKD
                     VFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRSFSIPNINVYD
                     ILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNPTIVMSEVETV
                     ISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKPDEDEFYYVSR
                     SIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            2733..3233
                     /locus_tag="ACHAZB_001818"
     CDS             2733..3233
                     /locus_tag="ACHAZB_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8614463.1"
                     /translation="MIKPMFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLS
                     SIQLLTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITK
                     TFLEKEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVN
                     EEEDFF"
     gene            3245..3739
                     /locus_tag="ACHAZB_001819"
     CDS             3245..3739
                     /locus_tag="ACHAZB_001819"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8614464.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            3760..4569
                     /locus_tag="ACHAZB_001820"
     CDS             3760..4569
                     /locus_tag="ACHAZB_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM8614465.1"
                     /translation="MYDAFVVALAVFIGIAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            4566..5387
                     /locus_tag="ACHAZB_001821"
     CDS             4566..5387
                     /locus_tag="ACHAZB_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8614466.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            5438..7537
                     /locus_tag="ACHAZB_001822"
     CDS             5438..7537
                     /locus_tag="ACHAZB_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HFM8614467.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            7534..8652
                     /locus_tag="ACHAZB_001823"
     CDS             7534..8652
                     /locus_tag="ACHAZB_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFM8614468.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            8691..9569
                     /locus_tag="ACHAZB_001824"
     CDS             8691..9569
                     /locus_tag="ACHAZB_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFM8614469.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            9715..9912
                     /locus_tag="ACHAZB_001825"
     CDS             9715..9912
                     /locus_tag="ACHAZB_001825"
                     /inference="COORDINATES: protein motif:HMM:NF016526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFM8614470.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
     gene            9896..>10111
                     /locus_tag="ACHAZB_001826"
     CDS             9896..>10111
                     /locus_tag="ACHAZB_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8614471.1"
                     /translation="MNFFGDRFFQPYKINRNSIIESCDYKKIARKKKKVSIHFKYFIP
                     ISIAKKVEILYLPLTLASLANVVSILID"
BASE COUNT         3094 a         1667 c         2267 g         3083 t
ORIGIN      
        1 gcagccgata gtttattatg cttttagaca cattaaactg gaggaaaaaa tgaaaaagag
       61 cgctaaagat cgagtttttg atgagctgat acgactaaca gggaatatgt cttctgtcac
      121 tgccgagaaa atttcgaaga atctaggtat ctctcgtcag aatgccagcc attatttgac
      181 gagattagtg gaagataaaa aggtcgagaa attaccggga aagcctgttt tgtggaagcc
      241 tttagatgaa tatgcggttg tagataatac ggagcaaata aacgaagcct ttcattctgt
      301 agttggtcat gacggaagtc tgagagaggt cattcaaaaa tgtatagccg ctgtcaaata
      361 tccgcctaac ggactcagtg tattaataaa tggagccacg ggtgttggaa aaagtttttt
      421 agccacaaaa atttttgaat acgctatcca tgagcaaatt attgaaaaag atgcaccatt
      481 tgctatctta aactgtgccg attatgcgga caatcccgaa ctgctttcgg caacactgtt
      541 tggatacaag aagggggcct ttacgggggc tgaaaaggat accgatggtt tattggcaac
      601 ggccaacaac ggctatcttt ttctcgatga ggtccatcgc ctatcgaaag aaaatcaaga
      661 aaagctcttt ctatttattg atactggaaa ctatcgtcca gtcggtgaaa atgtaaattg
      721 gcattctgct aaggttcgtt ttattttcgc cacgacggaa aaaggtgaaa attatttatt
      781 agacaccttt gatcggcgta ttcaaattag tgttatgttg ccaacttttg aagatcgccc
      841 cattcgagaa cgattagagc tgattcaatt attttttcaa aatgaagcgg atgttttaca
      901 aaaagatatt attgtttcaa gagaagcctt gtctttaatg ttatctcatc cctttagtgg
      961 gaatattggt aagctgaaga atatcattaa aatcagttgt gcggatgtgt atagtcgaac
     1021 acaagaagaa ccattaacga ttgggaaaaa tgagatcttg attcagttaa atatgctgga
     1081 atccgaagta gaaagcttgc cgattgctag tttattgata aagaaaaatc aaaagataaa
     1141 aacggatcga atcaaaccgt ttgattatga ttcgattttg gaaaaaatta ctcagctgat
     1201 aaatgaaggt aatttcaatg aaaatttttc ttcgataaag aatgaagtac agcatttggt
     1261 gcatcacatt gataaggatt cttttttatc aatgagtaga aaattgatga ttcagatgtt
     1321 tagaaaggtc tgggtatccg ttttaggaaa aacgtacgga ttgacgacga gtggacctgt
     1381 tgcagaaatt attagcagat cctatgtcat ttccccaagc tataagacag acttaaccgg
     1441 gataaatcgt acattagctg caaatctacc tcggacgact tacttatgta atcagttctt
     1501 ggagcattta ccaccgctct cagaaaagga cgagaacttt ctaagactgc ttattgtcgt
     1561 gactttatct gattatgtag atgaatccat cgagctgaag ggcctgttgc ttgctcacgg
     1621 agatagtacg gctagcagca ttcaagcagt agtgaatcaa ttatgcggca attatgtttt
     1681 tgaagcgttg gatatgccag tagagagtaa tgtgtcagag attgtcgaat cgacaaggaa
     1741 tttcgtgaaa cggcaagtga atacagatag tctagtgctg atcgtagata tgggctcttt
     1801 aaatcaaatt tacacacaga tcaagaacga tttaaaaggg gagttacttc ttctgaataa
     1861 tttgacgact tccattgcac ttgatattgg cttgaagatg acgatgaatg cgccatttaa
     1921 acagattgcg gaggatgcag cagacaagta ttcaatcaat gttcaatact ttgaaggctt
     1981 ttctcaaagt agcaacatta tcatttcctg tatgtcgggt ttggggattt cggacaaatt
     2041 gaaagatgtt tttcgtcatc atttatcgga tgaatccatc gaagttttca cgaaggacta
     2101 cagagaacta cgtgaattaa tcgaccaaaa tgatgaaact tattttgaca agacaaaatt
     2161 ggtcatcacg acttcggatc ttccacgttc cttttcaatt cctaatatta atgtttatga
     2221 catcttagac ggtgatggag cagccaattt ggagcatatt ttgagtcctg aaatgaagcc
     2281 ggcagaattt aaccgcttga ttcaggagct agtaaaattc ttttcgttgg aaggaattgc
     2341 ggaccgtttg aactttctga acccaacgat cgtgatgagt gaagtggaaa cagtcatttc
     2401 taaatatgaa aattactatc atttcacttt aaatggccgt gtaaaactaa atctctatat
     2461 gcatacggca ttgatgatgg aacgtttatt cttggctcgg acagaaaaat ctgaagaacc
     2521 gaattatgat ttgaaaccag atgaagatga attttattat gtttctagaa gtatttttca
     2581 atcaatggag atgaaatata attttaaagt caatgcttat gagctttcgc tgctctatga
     2641 attattagga ccgtatattc aaaaataaag tgtctaaaaa agtgtctaat ctgttttttt
     2701 taggttagac acttttttta atgtgaatta acgtgattaa accaatgttt ttaaaagttg
     2761 gcacaggaat tgcttatata aaagtgaaca gaaagaaagg aattaaccat atgaagaaaa
     2821 ttctaatagc gactcacggt cacttagctt caggctttct tagcagtatt caattactga
     2881 cggggaaggt tcaagagatt acggtgatta atgcttatgt ggatgagtgt gattttgaac
     2941 aggagttgaa tgtatttgta aaccaggtga acgagcagga tcagattttt gtttttaccg
     3001 atttatttgg cggaagtgtc aatcagaaga ttaccaaaac ctttttggag aaagagattg
     3061 ctgtaactat tttgtctggc tttaatctgc caattctgct cgaaatagta ttggcaacca
     3121 aggaattaac aaaggaggat attcaggaac tggtaaataa atgccaacag gaattaaaga
     3181 tgaccattct tgatgaggta catgtagtaa atgaagagga agattttttt taggaggaag
     3241 aaaaatgatt aaagcagtgc gaattgatga acgattaatt catggacaag tagcgatgac
     3301 ttggacaaag agtctaaatt taaccgggtt agtagtagca agcgatgagg cggctaccaa
     3361 cgatattcaa aagatgacat tgaagatggc tgcaccatcg aatgtcaaag tcattattaa
     3421 aacagttgat gacgcgattc atttgttaaa agatccgcgg gcgaaggaca tgcgcttgat
     3481 ggttttagtc cctaatgtca aagatgcggt aaaagttgcg aaagctttcc caacggaaat
     3541 cgaattaatg aacgtgggga atgctgggaa aatgacaccg ccatcggaag ataagaaaac
     3601 cttatcaaag gaagtgatgt taacgacgtc agaaattcaa gacttaaaag aattagttga
     3661 actttatcca gataccgttt ttcagcctac accggcgatg gaaaaacgtt cagctatctc
     3721 tattttgagt gatttttaga aaaaggaagg agaaaaatta tgtatgatgc atttgtagtc
     3781 gctttggccg tttttattgg aattgctgga catgaaatgt tcgggatgtc catgctgagt
     3841 cgtccgattg ttgttgctcc gttagcagga gcgttgatgg gggatttgca gacggggttg
     3901 attgtcgggg cgtcactaga agccatcttt atgggtgtcg tcaatatcgg aatctctagt
     3961 acggcggagc cttctttggc tgctgggctg gctacagcgt tcgcaatcaa aatgggagga
     4021 aacgtggatg cgattattcc cattgtcttt ccactagcag tattaggatt acagttaatg
     4081 aatatgattt tttcttttgt atgtggaccg atggctgcta aatttttcaa atttgcaaaa
     4141 tctggtgatc aaaaaggaat catcaatctg cacttcttta tgtggtttgt ccactatgcg
     4201 ctttatgctc ttattccatt ctttgccgtt ttatttggag cagatgttgt caaaagcgtc
     4261 ttggataata ttccagaggt catcatgaac ggattaacag tcgctggaaa tctgctgccg
     4321 gcagttggga tggcgatgct gttacggatg ctttggagta aaaatattgc tgtgtggttt
     4381 ttctttgggg ccgttgttat ggcgtatctc gagttaccat tgattgcaat tgccgcagtt
     4441 ggaacgatta ttgcaattgt tatagcccaa agagatctac aactaaaaaa tatatctgtt
     4501 catgcagttc aaagcgatgc tgctgtaaat gcaacgcttg accaagaaga ggaggatttc
     4561 tttcaatgaa attagctgat ggaatttcta aagatgagaa aaaattggtt cggaagatgt
     4621 tttggcgctc gatgactatg tatgcttcgg tgaatccggt gacgatgggc ggcggtggtt
     4681 tcgcctattc tatgatgccc ttcattaatc atttctacaa aagtgaagaa gatcgagctg
     4741 cggcgctgga acgtcataca cagtattttt cgacgaccat tccttgcgcg tcctttgtca
     4801 tggggattgc ggcatcgatg gagaaggaaa attctgaaaa agctggtttt gacgtggaaa
     4861 gtatcaacgc gatcaaaacg agtttgatgg gaccattggc tggaattggc gattcattgt
     4921 tctggggagt ttggcgtgtc gttaccgcgg gactagcgat caacttagcc aatgccggaa
     4981 atattttggc accaattatc tttttagtta tgttcaatat acctaattac ttgatgcgat
     5041 actatggcgg cgttttaggc tattcattag gctctaaata tattgaaaag ctgtatgcga
     5101 acgggttaat tgaaattctg acaaaagctg ctagtatctt gggtctgatc atggtaggtg
     5161 caatgacgat cagtatggtc aaattcaata ccgttttgag cttttcaatg aagggcgaag
     5221 aactaatgaa tgtccaagaa atcctcgata gtatttttaa aggaattgta ccgttattgg
     5281 taacattgag ctgtttcaaa ttactagata aaaaagtcgg cgtcatcaaa attatttttg
     5341 gaatggtcgt cttgggaatt ttattgtcat tacttggaat cgcatagtag gaagctgcag
     5401 ctactaggct gcagtttttt tagcaaggag ggaaaagatg actattaatg aagtaattta
     5461 ttcacccaaa cacccatttt ttaatgctga gttaaacgac ggaaatgtga ctacttcaaa
     5521 cgaatacagc tactatcgga atattcctga tgtaaaggga ttgaccttcg atgtaagtaa
     5581 tcaaaagaca ttagcaaacc ttgatgtttg cgggacgatt caaacgttga ccttttatcg
     5641 agaaaattta ctaactgagg aaaaaccagg tgtttgggtc aataaacagc tctctcaaac
     5701 caattcgtta tccttaaagg ttgaagtaaa cgggattgtc taccatttgt cagaatcgga
     5761 tcatcgtatt gaagtcgatg tgattcaaga ctgtttgcct agatatattc atcattattc
     5821 gacgtttaag gtcattgtag tatcctttgc tcccatactg gctaataagc gtttaagtat
     5881 gcttgttcaa caggtttaca ttgtgaatga aacacacgaa agccttcaag tttcagtgaa
     5941 agatgtgcct tcgtatcaag agaagtattc ggatcagcaa aatgtgttga tttctcaaaa
     6001 aggaaataag gacacgatcg cacaacagga agtggcaagc ttcgccattg cttttgtgga
     6061 cccaaatgct tttgaagaag taatgacttt tcaggaatct gatatcgaaa aatggctgaa
     6121 ggaaacattg aactattttg ctcagctatt tgggcagctg tcaatgccaa acgatagagt
     6181 tgtccatctc tatcgtcgtg cactttatca aagcttttct agtttcggta tgaatcgaca
     6241 gggacaggtc gtcggcagca attggggcag ctatcctgct actaatcgaa tttggaataa
     6301 ggatatgtat tacgcatcat tgccattcac catgtttgaa ccggagttat gtcaaaaaac
     6361 ggttctatgg tttgatcaat atggcgttaa gtttcctggt tcaaaattta caggaggtat
     6421 caatcattct ctgagtaatt cgttatccag tgcgttactg gctagtcttt attttgaaca
     6481 tactggggat acaaaattct ttgagcggtg tccgcaggtt ctactgaatg cttcaagaat
     6541 tattgaggac atattagctc aacgtcggtc aggggaacca atgctttttc catctgtttg
     6601 gttatcagac gcctttgcac ttggaaagta tcataccggc agcaatcttt gtctttggaa
     6661 ggcctgtatt ggacttgcag aaatttttga agccctagaa caactgagtc ttagcaagcg
     6721 atacaaaaac attgcctgta aactaaagga atcaatttgt aaacaaatga ctatcgatgg
     6781 tccttttggt gagcaatttt tggagggaat cggtgatgag gagaaaacaa cctattcagt
     6841 gaagcactat caaaaaccga ttcttgagca agggttaatc tttttaagtg atgtgattgt
     6901 ggatggaaaa attaatttat tgatgcatga tggagaagaa tcagacacta cgttgatgcc
     6961 tttttatcaa tttttagata ataaagatca tttatatcag aacaccatgg ccttttctgc
     7021 tagttctttc aatccaacct atagcgagga aatcaaaggc atcacttggg gattagaatc
     7081 cggcgccact tttcctggct ttatcactgt tttaatgagc gagctgcaca accctcaagc
     7141 tttcgtaacc cgattagaag aactaatttg tttggcagat ttagacggtt cgtggtggtg
     7201 gtggccgtat cgcttagagg ctcaacaagg agaagtcgtg cgagactttg gctgcggcaa
     7261 atgcggctgg gcatcgggtc tttttgtcag cctaatgatc agtcaatatt tcggattgaa
     7321 attcaaaaaa ggagtattgc aaattgatcc tgttgataat ttaacgtttt cttggaagaa
     7381 tttgaagttc ggtcaagcct tcatttcgat tgaatgtacg caatccgagt tgagtatttc
     7441 aaatttgtca gaaaagccat tgaaaataag tgatacgaaa aaaattcttc acgtagaaaa
     7501 gggaaaaacg attcacattc aaaggagaaa aagatgagta tactaaaaaa tattacccaa
     7561 tttaaaaaaa tccagagtcg agcaatcact cccgaaaatc caacaggcga gaaaggtaaa
     7621 gctgcgatgt tgccaagcga gctaggacca agccgcaaag gtcgtggcgc aattccgctg
     7681 acttctggtg aagaaactgt gattgcggat attactggaa cgggaataat tcgtcatatg
     7741 tggatgacga ttcgtgatcg aacagaattt ggcagttttg ttttacgtga tttaatctta
     7801 agaatttatt gggatgggtc ggatagccct gctgttgaaa gtcctttagg ggatttcttt
     7861 tgtaatgggt ttggagagcg ttgcgaggtg aactcgttgc cgatagtggt taatcctaca
     7921 gggggcttta attcctattt cgaaatgcct ttccaaacat ccgctaaaat tacgatcgag
     7981 aatttacatc cgaaggatat gtcctcattt ttttatacga taaattacgc tttggtggac
     8041 gaattggaag aaaatagcct atattttcac gcaaattggc atcgggaacg cattaccaaa
     8101 ttacaggagg attataagtt aattgatcga ttaaagggtc aaggctatta cgtgggtact
     8161 taccttgcgc ttacctgcct agaacgttat tggtggggcg aaggcgagtt taaattctac
     8221 attgatgggg atagtgagta tccgaccgta acctcaacag gttcagaaga ttattttggc
     8281 ggggcgtggg cctttcatga gtacgatgag ttcggacgtc cacatgcgaa aaactattca
     8341 acagcctttt tagggtatcc ttaccaatca aatagagatg ccacaagaga tcgttttgaa
     8401 acgggaaaat tgaatgccgt tcatgccttt ggtgacgacg caatgccacg acatggtctt
     8461 tatcgttggc atcttcaaga ccccatctgt tttaccgaag atttatcggt cactttacaa
     8521 caaatcggca acgatgatgt tcgcttattt gagcggcaag acgatgttgc tagtgtcgct
     8581 tactggtatt caacagaagc ttcaggaaat actaaacgtc atttcactga tcgagaacgc
     8641 ttgccgcgtt aatgaagtgt ttttcacata ttcgaaggga ggaacctatg atgatatatg
     8701 gggcaattga agctggaggg actaaatttg tctgtgcagt cggtactgag gaattaacga
     8761 ttttggaaag agtcagtttt cccacaacaa ctccagttga aacaatgaag caggtcattg
     8821 atttttttaa accttttaag gaacaattgg caggcatcgg ggtcggctgc tttggtccaa
     8881 ttgatattca atgcaaatca acgagtttcg gtcatatcac atcaacacca aaacttgctt
     8941 ggcaaaattt caattttgta ggaacgctaa agcaatattt caatattccg attgaatgga
     9001 caacagatgt caatgcggct tgctatggtg aatatgttgc tggttctggc aaagggttag
     9061 caggtgttgc atacttcact gtgggaacag gtatcggagg tggtgccttg ttcaatggga
     9121 ctttcgtgga agggttcagt catccggaaa tggggcatat gttgatcaag cgacatccaa
     9181 aggacaattt ttcaggaaac tgtcccttcc accaagattg tttagagggg atggcagcag
     9241 gaccagcgat tgaaaaacgg ttgggtgtaa aaggccagaa tttattggct gacgattcgt
     9301 tttggcagat tgaagccttt taccttgccc aatgtgcata caatacaaca ctgatgtttt
     9361 cgccagatag aattattttt ggcggtggtg taatgaaaca agaacacatg aagaaaaaag
     9421 ttcaggataa atttgttgag cttataaatg gatatgttga aataccacca attgatagct
     9481 acattattac gcccgaatta ggtgataatg ccggaataat cggcggatta gcattggcca
     9541 gaaaagcggt aagaaataag caaccataat taatgaatgt ttcattgata ctcatgtcaa
     9601 aagtatttaa ccgagtaatc aaatgatcaa atgaagttgg ataaagcgaa aatgaattca
     9661 aataaaaagg ctctaagtca aatcgtgttg ttttcattaa atcactgaaa ggaaatgctt
     9721 ctgcgtttcc ttttttcttt ttgttgtgcg ctgtatacct tgaggatatt cactggtggc
     9781 gtacacttaa atagtaatat attgtgttac tttatgtctg tggtaatagt tatagttgct
     9841 taccaatgta tgttcatttt tcgaaatgtt gacttcataa tccttggctt aatgtttgaa
     9901 tttttttggt gatagatttt ttcagccgta taaaataaat cgtaattcta ttatagagag
     9961 ttgtgattat aaaaagatag caagaaaaaa aaagaaagtt tctatacatt ttaaatattt
    10021 tattcctatt tctattgcta aaaaagtaga aattctatat ttacctttaa ctctcgcttc
    10081 tcttgcaaat gtagttagca ttttaatcga t
//
LOCUS       DAYYCY010000090        10107 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000090 DAYYCY010000000
VERSION     DAYYCY010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10107
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.090"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            99..746
                     /locus_tag="ACHAZB_001827"
     CDS             99..746
                     /locus_tag="ACHAZB_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFM8614472.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    875..1091
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1139..3784
                     /locus_tag="ACHAZB_001828"
     CDS             1139..3784
                     /locus_tag="ACHAZB_001828"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFM8614473.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4094..5407
                     /locus_tag="ACHAZB_001829"
     CDS             4094..5407
                     /locus_tag="ACHAZB_001829"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HFM8614474.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5397..6050
                     /locus_tag="ACHAZB_001830"
     CDS             5397..6050
                     /locus_tag="ACHAZB_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8614475.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6105..6656
                     /gene="radC"
                     /locus_tag="ACHAZB_001831"
                     /pseudo
     CDS             6105..6656
                     /gene="radC"
                     /locus_tag="ACHAZB_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
     gene            6787..7872
                     /locus_tag="ACHAZB_001832"
     CDS             6787..7872
                     /locus_tag="ACHAZB_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8614476.1"
                     /translation="MKVQRIEVENKPYPLYLLLDKEYQLIEPVMKFIKYLDNTGKSPN
                     TIKAYCYHLKLLYEFMEQRGVILNDINFELLADFVGWLRYPSASNVIDLQSKKAIREE
                     TTVNTILNVVMSFLDYLSRLGEFKSIDVFKQAKGRNFKGFLHHVNKGRYQKNVLKLRV
                     KKKQIRTLRSEEVKQIIDACHTKRDKLILMLMYEGGLRIGEVLSLRLEDIVTWDNQIH
                     LTPRDVNVNEAYIKLRKERTIHVSKELMSLYTDYLIYEYSEELEHDYVFISLKEGYFG
                     KPLKYQSVLDLVRRIVKRTGIEFTSHMLRHTHATQLIREGWDVAFVQKRLGHAHVQTT
                     LNTYVHLSDQDMKNEFNKYLERKEHKK"
     gene            7869..9761
                     /locus_tag="ACHAZB_001833"
     CDS             7869..9761
                     /locus_tag="ACHAZB_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFM8614477.1"
                     /translation="MNASSKRKIISQSEISKKIAVMNEEMQGFWANNSWDIRKCPHPS
                     AIELSKNPALRNRWVRFERVKNLWLRTELKYFYFYHLNNGIWNAKTVWIRKGTVINKM
                     LDFLDLKYPSITSITEVPIEKAMTEYRTYLTKRGVRITTTNYKITANQEKTPVKANSY
                     YVTNLKQFMEFYENFYFDGEEWDKDVWDRRNLPLPDDKVNPTQYEYTINFKGFRNTYF
                     KQLVKRYCKLRLNMDSFSYVSDIAQRLKEFFNFLDMKFKQVQRVHQLTRVEIEAYLSE
                     LNMMGIKPSTITGRISILEGLFSTLLRLEWDDVPSKILIYSEDYPKIPRAKPRFIDEF
                     VLEQLNSHLDKLPEYIATMTMIVQECGMRISELCTLKKGCLLEDKDGDFFLKYYQWKM
                     KKEHIVPISKEVALLIKVREDKVSEEFPDSEYLFPRKDGSPLKQETFRGELNKLAYEQ
                     NIVDKSGEIYRFHAHAFRHTVGTRMINNGMPQHIVQKFLGHESPEMTSRYAHIFDETL
                     KNEFTKFQEKLVTNNGDVLDLDEDNEVDDVELQWFKKNINAQVLPNGYCRLPVVAGGC
                     PHANACLDCTHFCTSKQFLPQHEEQLERTEELLAIAKDKQWQRQVETNSRVKERLEQI
                     IGSLTG"
     gene            9768..>10107
                     /locus_tag="ACHAZB_001834"
     CDS             9768..>10107
                     /locus_tag="ACHAZB_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6262 family protein"
                     /protein_id="HFM8614478.1"
                     /translation="MDKQVRNTTEIVRLAKQKSKKTREKVDKAISKFSIEGKVINFNS
                     IAKEANVSKSWLYKEHDIRQRIESLRERQITANVVSKPKKSSRSEEILIKTLKRRVME
                     LEKENKKLQNQ"
BASE COUNT         3511 a         1576 c         2142 g         2878 t
ORIGIN      
        1 atttgtctca cacccttttc cttcctactt gtaaccttgc tcccgtgtta gaaacatgtt
       61 acactaaata tgtgaaatta ttaacaagga gcgtcgttat gcgagaaaaa acagatacac
      121 tgccaaaagc aactgcgaaa agattacctt tatatttacg ctacttgaag atgctggatg
      181 actcaggtat aagtagaatc aaatcaaatg agttcagcga aatcacccaa attccttctg
      241 caacgatccg cagagacttc tcgcaattag gcgagctagg cagaagcgga tatggctatg
      301 atgtcccttt tcttatcgat gtattcaata atatccttaa tactaaagaa gaaaaacgaa
      361 tcgcattagt aggatatgga aatcttggca aagcattgaa acacaacaat tttagaagaa
      421 atgaaaatct aaatatcgtg tgcgtctttg ataatgatcc agcactgatc aatcgagtaa
      481 ttgatggaga aatgatctat ccgattgatc gtttcgcgga gatagccaaa gcaaaaaatg
      541 tgacagtagc aatctctacg gtaccaagta aatattcaca atctgctatt gatgaaattg
      601 tcaaaggaaa tgtaaccgct atacttaatt ttgcgcctga cagagtaact gtcccagctt
      661 atgtcaatgt tcaatacatt gatttgacaa ccgagttgca gacgcttatt tactttgatg
      721 agaattactc agaagttttt tcttaggaaa atttgagtag gcttattgat cttgcgagaa
      781 ctcgtcgtaa ttctttcaaa aattttttag tactaaatga agtggttttt tcacgaccac
      841 ttgttgacta aaaaaaaaaa ccattgtaga atgaagcata acgctgaaca agaggagtaa
      901 ctggcacatt cattcttaga gagagaacta tctgctgaaa ggttctcaat gacaaccagt
      961 gaaggtagct tgggagtttc tgtcttgaat ggagtagaga tagacgagtc gtgatcgtta
     1021 actcacttga gaggcaataa tgattgatta ttgcaattta ggtggtaccg cgtaaatcat
     1081 acgtcctaga atacgaaagt attttaggac gttttttagt ttaaaggagg aaaacagcat
     1141 gacggaagaa aataacctat caacaaaata taatccgcaa gaagttgaag ctggacgtta
     1201 tcagcattgg ttagaacaag atttgtttaa accgagcgga gataaaaaag caaaacctta
     1261 ttcaatcgta atcccgcccc caaatgttac tggaaaactt catttagggc atgcttggga
     1321 tacgaccttg caagatatgc tgattcgtca aaagagaatg caaggatatg atactttatg
     1381 gctgcctggt atggaccatg caggaatcgc gacacaagca aaagtagaag cgaagcttag
     1441 agagcaaggg atttctcgtt atgatctagg tcgtgaaaag ttcatcgatc aagtgtggga
     1501 atggaaagaa gagtatgcga gccacatccg cgaacagtgg gctaaaatgg gcttatcttt
     1561 agattacagt agggaacgtt ttacattaga tgatggatta tctgaagcag tacgtaaagt
     1621 ttttgttacc ttatacgaaa aaggtttgat ttatcgtggt gaatatatca tcaactggga
     1681 tcctcaagca cgaacagctc tatctgatat cgaagttatt cataaagata tcgaaggcgc
     1741 cttttatcat atgagctatg aactagcaga tggatcaggc gtagtagaaa ttgcgacaac
     1801 ccgtccagaa acgatgttgg gagatacggc gattgctgtt catccagaag acgagcgtta
     1861 tcaagcgctc atcggcaaaa aagtgattct acctttagtc aataaacaaa tcccaatcat
     1921 tgcagatgaa tatgtagata tggaattcgg gacaggtgta gtaaaaatca ctccagcaca
     1981 tgatccaaac gactttgagg taggtaatcg tcatgatctt ccacgcgtaa atgttatgga
     2041 tgatgatggt acgatgaatg aactagcagg aaaatatgct ggaatggaac gttttgctgc
     2101 tcgtaaagca atcgtgcaag atctgaaaga aatcggtcga ttgattaaaa tcgatacgat
     2161 gatccacagt gtgggtcatt ccgagcggac aggtgtcgtt gttgaacctc ggctgtctac
     2221 tcaatggttt gtaaaaatgg cgccgttagc gaaaaaagcc attgataacc aagaaacaga
     2281 tcaagcagtg gaattcttcc ctccaagatt caatcaaacg tttttacgtt ggatggaaaa
     2341 tgtccatgat tgggtaatct cacgacaatt atggtgggga catcaaattc ctgcatggta
     2401 tcacaaagaa acaggtgaaa tgtatgtagg gatggaagca ccagcggata gtgaaaactg
     2461 ggtacaagat cctgatgttt tagacacttg gtttagttca gctttgtggc ctttctcaac
     2521 aatgggctgg ccagatacgg aaagcgaaga ttataagcga tacttcccta ctagcacttt
     2581 agtaaccggt tatgacatca ttttcttctg ggtaagcaga atgattttcc aaagcttgga
     2641 atttactggc gaacgtccat ttgagaatgt cttgattcat ggattgattc gtgacgaaca
     2701 agggcggaag atgagtaaat cattaggtaa tggaatcgat ccgatggaag taatcgaaaa
     2761 gtatggagca gatgctctcc gctggttctt atcgaatggt tctgcaccag gacaagatgt
     2821 ccgcttcagc tatgaaaaaa tggatgcttc ttggaacttt atcaacaaaa tctggaatgc
     2881 ttcacgtttc gttatcatga atatcgaagg aatgaccgta gaagagatcg atttatctgg
     2941 agaaaaatca gtggctgacc ggtggatcct tactcgtttg aatgaaacga tcgaacgagt
     3001 aactgagcta tttgatcgtt ttgaatttgg tgaagcaggt cgacaactgt ataactttat
     3061 ctgggatgat ttttgtgact ggtacatcga aatgagtaaa gaagtacttt atggagatga
     3121 tgctgtttca aaacaaacca cccgtagcat cttagtccat acacttgatc agatcctacg
     3181 tttattgcat ccaatcatgc cattcgttac agaagaaata tgggaacata ttcctcatca
     3241 gggaaattct ctagtagtgg cagaatatcc tgttgtacgt ccagagtttg atgatgaaac
     3301 agcttctaaa gggatggaag tattgaaaga attgattcga gccgttcgaa atattcgttc
     3361 agaagtgaat acaccattgt ctaaaccaat tacgttgcta attaaaacaa acgatgctaa
     3421 aatcgatcaa tttttagttg aaaatacaag ctatatcgaa cgcttctgta atcccgaaga
     3481 gttgacgata tcaagtgaga tcactgcgcc agatctagcg atgtcagctg ttctgacagg
     3541 tgcagaaatc ttcttgccgt tagcaggatt gatcaatatt gaagaagaaa tcaaacgatt
     3601 agaaaaagaa ctggaaaagt ggacaagcga agtaaaacgt gtccaaggaa aactttctaa
     3661 tgaacgtttt gtttctaacg cgccggaaga agtagtagag gcagagcgtg caaaagaaaa
     3721 agattattta gagaagcaaa cggctgtcaa agaacgaatc gaaagtttgc gtactattca
     3781 ataagaatag aaaaagagag tgtgaaatca gctagctatt actaaaagct agctgattga
     3841 acatcgttac aggaaagagt ttgagcctca ctcttagctt cgattcataa ggagatgtga
     3901 cataaatttt gtcgcatctc ttttttagtc tactcttatt atgtaggaga aggagggagg
     3961 tggatcatgt atcaagagtc aataaagtag ttgataaaat cgttcctctc tctgtttctt
     4021 ttctctttgt catactttta tttttcttat gtgttctatt tgttataata aaagagaaat
     4081 gggggaattg ttgatgacga ttgaagaagc aatagaatgg atccatagtc gattaccatt
     4141 tggttcacgt cctggccttg accgagtcga agctttatta gaagtggtag ggcatccaga
     4201 gagaaaagta aaaacgatcc atatcgctgg aacaaacgga aaaggttcta ctgtagctta
     4261 tttgagaagc ctattagaag aggctgggct gacagtaggt acatttactt caccttatat
     4321 cgaaacgttc aatgaacgga tcgcaatcaa tggcaaaagc attccagaca atgatttgat
     4381 ccagcttgta aaaaaatatc agccactgat caaacaactt gatcaatttg aagcagtagc
     4441 tggtattact gaattcgaga cattaacagc aatggcattt gattattttt tgatgaagaa
     4501 agtggacgta gcgattatcg aagttggctt aggcggctta ttggattcta ccaatgtagc
     4561 tgtaccaatg ctgacgggaa taacaacgat tggacttgat cataccgaaa tacttggaga
     4621 tacaattgaa gaaatcgctg cacaaaaagc tggaatcatc aaacaaggcg ttccagtagt
     4681 aacaggaaat attgccccag aagcttttac tgtcatccaa caaacagcta aagcaaaaaa
     4741 tgcaccgctt tatgtatacg gggaaaatta taaaataaac tatctgcatc ctgatgagat
     4801 gtggggagaa gtatttgaat tttttaatga aataggaaag atccctcaat taaccactcc
     4861 gcttttaggg caacatcaag ctgaaaatgc aggagtagcc atcgaattgt attttgttta
     4921 ctgtcaattg gaaaagctgc ctttccgaga aaaagatgtt ttgaagggat tgaagaaatc
     4981 tcgatggcca ggaagaatgg agcgtttgag taacgagcca ttagttgttt tagatggtgc
     5041 ccataatacg catgcaatga accggcttgt tgagaatttg aaaaaagaat tcaaaggata
     5101 tcaaatcaac attttgtttt ccgccttacg aactaaaaat gttagagaaa tgctagaacg
     5161 attgcagcaa gtaccaaatg tccatattta tttgacgacc tttgaacatc caaaagccat
     5221 cgaattaagt gaagtatcag atttagcaag tgagagagta tcaatcgttt cattgtggca
     5281 attcggatta gcagatatat tggaaaagat gacaaatgaa gacgtattag ttgtgaccgg
     5341 ttcactttat ttcatttcag aagttcgcca tttattaata caattaggag gagatcatga
     5401 agtataatgc agtgattttt gacatggatg gtttgctgtt cgacacagag ttagtgtatt
     5461 acgaagccac acaagtagta gcagacaaga tgggttttcc ttatgataaa gacctgtatt
     5521 taaaattctt aggtgtttca gatgaagaag tatgggcgaa ttatcatatg attttctcgg
     5581 aatttggcaa aacaagtgta caaagattca tagatgattc ataccaggaa acgcttgaac
     5641 gtttttcgca aggagcggta caaatgaagc ccggtgtgtt ggaactcctc tcttttttag
     5701 aagaacatca gattcccaaa gttgtcgctt ccagtaacca acgagcagtc attgaacttt
     5761 tattgaagaa gaacaatctg tttgaacgat ttgagacgat tgtttctgca gaaaatgttc
     5821 aacgagcaaa accagaccca gaaattttct tgttagctaa tcagaagcta ggtacagcga
     5881 agaaagaaac attgatttta gaagattcac aaaatggtat tttagcagct tctgcggctg
     5941 agatcccggt agtcatggta ccagatctgc ttccaccatc ccaagaattg atcaaaaaaa
     6001 cagctggggt tgtttcttct ttacatgaaa tccctgcatt gttaaaataa aaatccatct
     6061 cttttcgtat cttattttag ataagataga aaggagatgt ttttttggga aaacgattga
     6121 tcaaagaagt acctactagc tctttaccaa gggaacgtat ggaaatttat ggcgcggaag
     6181 ctttatcgga tcaggagctt ttggcaatct tactgcgtac cggtcaacac ccttatagtg
     6241 tgatgtcgat tgctggaaat ctgttaaaaa cgtttggagg actggcctct ttacgacagg
     6301 ctactttgca tgagttagaa gagattcgag ggataggacg cgtgaaagca atcgaaatca
     6361 aagccttgat cgaattaggt cgacgaattc agacggatca tgaaagagcg gctccaccgg
     6421 ttcgtaccag ctacgaattg gcccagcaat tgattctgga aatgaaagat cacaaacaag
     6481 aacatttggt ttgtatttat ttggacacga agaatcaagt gatcttaaaa aaaacagtgt
     6541 tcatcggttc gctgaatcag tcagtcgctc atcctagaga aattttccat tatgctgtac
     6601 gttattgtgc agcaaggatc gttttagcgc ataatcatcc ttctggtaat gttttaacca
     6661 tagtataata aataatagta gttaaagtag tttaagagga gatattaaag ggatatttta
     6721 ctttaaataa aaattaacaa ggtttataat ttgtagtagt taaagtattt aaagaggtgg
     6781 gaacatgtga aggttcaaag gatagaagtg gagaataagc cgtatccatt gtatttatta
     6841 ctagataaag aataccagct aatagaacca gtaatgaaat ttattaaata cttagataat
     6901 actggtaagt ctcctaatac cattaaagca tactgctatc atttaaagtt gctgtacgag
     6961 ttcatggaac agagaggtgt tattcttaat gatattaact ttgagttgtt agcagacttc
     7021 gtaggttggt tgagatatcc ttcagcatca aatgtaattg atcttcagtc aaaaaaagcc
     7081 ataagagaag aaacgacagt gaatacaatt ttaaatgtag ttatgagttt tcttgattat
     7141 ttaagtagat taggagaatt taaatcaatt gatgtattta aacaagcaaa gggaagaaat
     7201 ttcaaaggat ttttacatca tgttaataag ggtagatacc aaaagaatgt cttaaagtta
     7261 agggttaaaa agaaacagat aagaacattg agatcagagg aagttaagca aattattgat
     7321 gcttgtcata cgaaaagaga taaattaatt ttaatgctta tgtatgaggg tggtttaaga
     7381 atcggtgaag tgttatcgct taggcttgaa gatattgtca cttgggacaa tcaaatccat
     7441 ttaacaccta gagatgttaa tgttaatgaa gcttatatta aattaaggaa ggaaagaaca
     7501 atacatgtga gtaaagaact tatgtcactt tatacagatt acttgatata tgagtatagt
     7561 gaggaattgg agcatgatta tgtttttatt tccttaaaag aaggctattt tgggaaacca
     7621 ctaaagtacc aaagtgttct tgatctagtt agaagaatag ttaaaaggac tggaatagaa
     7681 tttacatcac atatgcttcg ccacactcac gcaacgcagc taattaggga aggatgggat
     7741 gttgcgttcg ttcaaaagag attaggtcac gcacatgttc agacaacgtt aaatacctat
     7801 gttcatcttt cagatcagga tatgaaaaat gagtttaata aatacctcga gagaaaggag
     7861 cataagaaat gaatgcttct agtaaaagga aaattattag tcagagtgag attagcaaaa
     7921 aaatagctgt aatgaatgaa gaaatgcagg ggttttgggc taataatagt tgggatataa
     7981 gaaaatgtcc acatccttct gccatagaat taagtaagaa tcctgcttta aggaatcgtt
     8041 gggttcgttt tgaacgtgtt aaaaatctgt ggttaagaac agaattgaaa tatttttatt
     8101 tttaccattt aaacaatgga atatggaatg caaaaactgt ctggattaga aaaggaacag
     8161 taattaataa aatgttagat ttcttagatt taaagtatcc tagcattact tcaattactg
     8221 aagttcctat tgaaaaagca atgacggagt atagaactta tttgacaaaa cggggtgtta
     8281 gaattaccac tactaattat aagattactg ctaatcaaga aaaaacacct gtaaaagcta
     8341 attcctacta tgttactaat ctaaaacaat ttatggagtt ttatgagaac ttttattttg
     8401 atggagagga gtgggataaa gacgtttggg atagacgtaa cttacctttg ccagatgata
     8461 aggttaaccc aacacaatat gaatatacaa ttaactttaa agggtttcgg aatacatatt
     8521 ttaaacaact tgtaaaaaga tattgtaagt tgagattgaa catggatagc ttttcctatg
     8581 taagtgatat tgcccaaaga cttaaagagt tctttaattt tctggacatg aaatttaaac
     8641 aagttcagag agtacaccaa ttaacgagag tggaaattga agcatattta agtgaactaa
     8701 acatgatggg aataaaacct agtacaataa ctgggaggat ctctatattg gaaggactat
     8761 ttagtaccct tcttaggcta gaatgggatg atgttccttc caaaatatta atttattctg
     8821 aggactatcc gaaaatacca agagcaaaac cacgctttat agatgaattc gtcctagagc
     8881 aattgaacag tcatcttgat aaattacccg aatatatagc tacgatgact atgattgttc
     8941 aagaatgtgg aatgaggata agtgaattgt gcaccttgaa aaaaggctgt ctattagagg
     9001 acaaagatgg agatttcttt ttaaagtatt atcaatggaa aatgaaaaag gagcatatag
     9061 ttccaatatc taaagaggta gctttactta ttaaagttcg ggaagataaa gtttcagagg
     9121 aatttccaga tagtgaatac ctctttccaa gaaaagatgg atcgccatta aaacaagaaa
     9181 catttagagg tgagttaaat aaattagctt atgagcaaaa tatagtggat aaatcaggtg
     9241 agatttatag attccatgcc catgcctttc gccatacagt aggaacaaga atgattaaca
     9301 acgggatgcc ccagcatatt gtgcagaaat ttttggggca tgaaagccca gaaatgacaa
     9361 gcagatacgc tcatatcttt gatgaaactc taaaaaatga atttactaaa tttcaggaaa
     9421 aactggttac caataatgga gatgtgcttg atctagatga agataatgaa gtcgatgatg
     9481 tagagcttca atggttcaag aaaaatataa atgcacaagt gcttccaaat ggttattgta
     9541 gattgccagt agtagcaggt ggttgtccac atgcgaatgc atgcttagat tgcactcact
     9601 tctgtaccag taagcaattc ttaccacagc acgaagaaca gttagagcgt acagaagagt
     9661 tattagccat agctaaggat aaacaatggc aaagacaagt agagactaat agccgtgtta
     9721 aagagcgttt agaacaaatc attggaagtt tgacggggta attatcaatg gataaacaag
     9781 ttagaaatac aacagaaatt gtacgtttgg cgaagcagaa atcaaaaaag acaagggaaa
     9841 aagtagacaa agcgatttct aaattttcga ttgaaggtaa agttattaat tttaattcaa
     9901 tagcaaagga agctaatgtt tctaaatcat ggctttataa ggaacacgat attaggcaaa
     9961 gaatcgaatc ccttcgtgag cgtcaaataa cagcaaatgt agtctcaaaa cccaagaaaa
    10021 gttctcgttc ggaggaaatc cttattaaaa ccttaaaaag aagagtaatg gaattagaaa
    10081 aagaaaataa aaaattacag aaccaaa
//
LOCUS       DAYYCY010000091        10074 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000091 DAYYCY010000000
VERSION     DAYYCY010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10074
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.091"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            152..382
                     /locus_tag="ACHAZB_001835"
     CDS             152..382
                     /locus_tag="ACHAZB_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 family protein"
                     /protein_id="HFM8614479.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            479..1780
                     /gene="murA"
                     /locus_tag="ACHAZB_001836"
     CDS             479..1780
                     /gene="murA"
                     /locus_tag="ACHAZB_001836"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFM8614480.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1793..1969
                     /locus_tag="ACHAZB_001837"
     CDS             1793..1969
                     /locus_tag="ACHAZB_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFM8614481.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2104..2400
                     /gene="yidD"
                     /locus_tag="ACHAZB_001838"
     CDS             2104..2400
                     /gene="yidD"
                     /locus_tag="ACHAZB_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFM8614482.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFQLTRNKKDKKEPDYKKVVKNTSSTER"
     gene            2464..2931
                     /locus_tag="ACHAZB_001839"
     CDS             2464..2931
                     /locus_tag="ACHAZB_001839"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8614483.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVAPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3016..3900
                     /locus_tag="ACHAZB_001840"
     CDS             3016..3900
                     /locus_tag="ACHAZB_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFM8614484.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNSYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4014..4250
                     /locus_tag="ACHAZB_001841"
     CDS             4014..4250
                     /locus_tag="ACHAZB_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HFM8614485.1"
                     /translation="MMSKKNKDVEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4338..4418
                     /locus_tag="ACHAZB_001842"
     tRNA            4338..4418
                     /locus_tag="ACHAZB_001842"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4372..4374,aa:Tyr,seq:gta)
     gene            4425..4496
                     /locus_tag="ACHAZB_001843"
     tRNA            4425..4496
                     /locus_tag="ACHAZB_001843"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4457..4459,aa:Gln,seq:ttg)
     gene            4621..4791
                     /locus_tag="ACHAZB_001844"
     CDS             4621..4791
                     /locus_tag="ACHAZB_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8614486.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
     gene            5011..5718
                     /locus_tag="ACHAZB_001845"
     CDS             5011..5718
                     /locus_tag="ACHAZB_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8614487.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5778..6422)
                     /locus_tag="ACHAZB_001846"
     CDS             complement(5778..6422)
                     /locus_tag="ACHAZB_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HFM8614488.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6549..6836
                     /locus_tag="ACHAZB_001847"
     CDS             6549..6836
                     /locus_tag="ACHAZB_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8614489.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            6944..7264
                     /locus_tag="ACHAZB_001848"
     CDS             6944..7264
                     /locus_tag="ACHAZB_001848"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8614490.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7345..8658
                     /locus_tag="ACHAZB_001849"
     CDS             7345..8658
                     /locus_tag="ACHAZB_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFM8614491.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEIAKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            8655..9374
                     /locus_tag="ACHAZB_001850"
     CDS             8655..9374
                     /locus_tag="ACHAZB_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFM8614492.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            9502..10053
                     /gene="raiA"
                     /locus_tag="ACHAZB_001851"
     CDS             9502..10053
                     /gene="raiA"
                     /locus_tag="ACHAZB_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFM8614493.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADATALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
BASE COUNT         3358 a         1666 c         2150 g         2900 t
ORIGIN      
        1 gtcccttttt ttgcatattt ttagcattta tttcctaaaa aatgtaaggg gattcctata
       61 tttttaagta tacttgtttt tccttttata cattttatgg tattatcatt ttattagtta
      121 ttataaaaca atcgttagaa aggacgtcat tatgcaagta tacggaattg atgcggtgct
      181 tcgaatcgtg agtcatttgg cgtttatcta cttagccttc tggtctttac gttcgttacg
      241 cattgaaact tttttcagac caatgcataa tacgcaaatt agaatggcaa tcgtactttt
      301 ttcaattttt ttagggtaca ctgcaagtaa ttttttctta gaagttatcg ctttatgccg
      361 aaatcttttc gttagcctgt aaattttaaa aaaaggtact attttaaaag caaggatgtt
      421 ataatagaaa agattttgag taagtgtatg ctcattggaa ttttttggag ggaacaacat
      481 ggaagagatc atcgtaagag gtggcaatca attaaatgga acagtaagaa ttgaaggagc
      541 taaaaatgct gttttgccta ttttagctgc tagtttgtta gcagaagaag gtattacgac
      601 attagacaac gttccgattc tttctgatgt cttcactatg aatcaagtga ttcgtcactt
      661 aaacgtagat gtagattttg atgaacaaaa gaatcaagta acaatcgatg cttcaagaca
      721 attagaaatc gaagcgcctt atgagtatgt tagtcaaatg cgtgcttcaa tcgttgtgat
      781 gggtccgcta cttgccagaa atggacatgc aaaagtagct atgcctggtg gatgtgcgat
      841 tggtaaacga ccaatcgatt tgcatttaaa aggatttcaa gctttaggag caaaaattat
      901 tcaaaaaaac ggctatatcg aagcaattgc ggatgaattg atcggtaaca cgatttattt
      961 agatttccct agtgtaggtg ctactcaaaa tatcatgatg gctgccgtaa aagcaaaagg
     1021 aacaaccatc attgaaaacg tagctcgcga accagaaatc gttgatttag caaacatttt
     1081 gaataaaatg ggcgctcaag tctatggagc tggtactgaa acaatgcgta ttgaaggcgt
     1141 ggatcattta catgcagtca accactcaat cgtacaagat cgtatcgaag caggtacctt
     1201 tatggttgct gctgcaatga cacaaggcaa tgtgctgatt gcagatgcta tctctgaaca
     1261 taaccgccca ttgatttcta aattgatcga aatgggtgca gaaatcattg aagaagaagg
     1321 cggagtccgt gtcatcggac caaaacatat cttacctaca gatgtaaaaa cgatgcctca
     1381 tcctggtttc cctacagata tgcaagctca aatgacagct atccaattag ttgcagaagg
     1441 tacaagtgta gtgactgaaa cagtgtttga aaatcgtttc caacatttag aagagatgcg
     1501 ccgtatgaac gctcatgtca aaatcgacgg aaatgtagca atcatggatg gaaaccatga
     1561 attgcaaggt gcagaagtat atgcaacaga cttgcgggca gcagcagcat tagttttagc
     1621 tggcttgaaa gcaaatggaa tcacacgtgt gcgtaacttg aattatctag accgtggata
     1681 ttataatttc catatcaaac tacagcaatt aggtgcagac gttgaacgtg tagacatgga
     1741 tcaaacttct gctgaaaaaa cagcacaaac gattgcttaa ggaagtggct gaatgagtaa
     1801 ccaacgatat atcttattaa ctttgataaa gattttagtg gtgatcttat tgcttatcct
     1861 tctttttgtg gccggaacga tgatcggata tggtgtcatc ggtggcggca atccatttaa
     1921 agtattccag ccaagtttgt ggattcatat tcgagacttt tttcattgat aaataagaat
     1981 cacagtggtt ctagtcgata tgaagtgagc catgaagcag aacatgacaa aagaaaacag
     2041 aagggaacgt tgacgttagt cgcgatccct ttttttagta aaagaaaaga aggaatctaa
     2101 ttgatgaaga agatattgat ttatttagtt agaggatatc agcgctttat ttcgcctgca
     2161 tttccaccta gctgccgtta ttacccaacc tgttccaatt atatgataaa agcaattcaa
     2221 acccatggtg cactaaaagg gacggtaatg gggactgcac ggatcttacg ctgtcatcct
     2281 tttgtcaaag gcggtatcga ttatgtacca ttgacattcc aattaacgcg aaataagaaa
     2341 gataaaaaag aaccagatta taaaaaggtt gtgaaaaata cgagtagcac tgagcgataa
     2401 agaaaaagag gaaaattatt gtcaaagtaa caactccgca actacttata tgggggacaa
     2461 aaaatgatcg aagaactaaa caagaaagat ttaccatggt ccttgttatt agatgcagat
     2521 cctgataaag aaaaagttca agtatatgtt tccagaggga gtggctggat atggaaagaa
     2581 aaagaaaaaa caataggtgt actgatctat gtggcaaggg aaaaagaata cgaaatcgtc
     2641 aatgtagcag tagccccaac gtatcaagga aaggggattg gcggaaaatt acttgagaca
     2701 gcttttcaga agctttcaca actcgcttcc agtcaaactc agatcatcat acgaaccggc
     2761 agtactagca gtgcggcgtt acatctctat caaaaaatgg gctatgtgga gattggtcga
     2821 gtcaaagact attttatcca tcattactct gagcccattt ttgaaaatgg agatcaatta
     2881 agggatcaag taattttagc aattcactta cctaaaaata gctttaacta ataaactttc
     2941 aattcgagaa aatgctttca aatgctaaat agatacatta caatagcttt agcaaacaaa
     3001 aaggagaata tttttatggg aatattagtt tttgattttg gtggatcggc tgtcaaatat
     3061 ggttgttggg atggcaaaga gattaaaggt aagggacaat ttgcaacgcc tgaatcttgg
     3121 gaagaaatga aagcgcaatt atttcaagtt tataaaaaaa tactcgtttc ttttgaagtc
     3181 gaaggaattg gaatcagctc tcctggagta gtgaataatg agaaacaggt gattgaggga
     3241 atcagtgcga ttccttatat tcatggattt aatattttcc gagatttgga aactctgttt
     3301 caactcccag taacgattga aaatgacgcc aactgtgccg gaatggctga attttatgaa
     3361 ggtgctgccc gtgattatca agatgttgct tttgttgtgg caggaactgg gattggcggg
     3421 gcgttgttca cgaaaggaca aatcaataaa ggggcacatc tttatggtgg agaatttggg
     3481 ttgatgttct taaatggcga taagacattc agtaaacttg gtacagcggt acagatggct
     3541 tggcgttatt gtgaacgcaa aggtctcgat aaaaattctt ataccggaaa agatgtcttt
     3601 gaattagcag aaactggaga tgcaatagca aaagaagaag ttgagagttt ttatacgtat
     3661 cttacaaagg gattattcag tattcaattt tcgtttgatc cagaagtgat cgtattaggc
     3721 ggcggtgttt ctgcaaaaga agggctcatt gatgagatca aaagccgaat gaagaagctg
     3781 acagaacaat tcgatctgca tgatttcgaa ccgcagattt tattatgtga atacagaaac
     3841 gatgctaatc ttgtaggtgc agcagcaaac tatctcgctg tacgtcaagg ggagaattaa
     3901 gagctttaga gaaggtgcag cttgcataaa agaagtgaat gaaatgaata ggagattttt
     3961 acctaaggaa tgttttattt gctgcagtat gataaaatat ctgagaggtg aaaatgatgt
     4021 caaaaaaaaa taaagatgtt gaagtgcgta tagaagaagt gaaaaaagat atcaaaggta
     4081 agaattatga agtcactcag ttatttatag ggaaaaaaat gattggtgag atcttagcct
     4141 atgggccaaa agaatatgaa attttctttg gtgaagaaga ttttggaaaa gaaaaaagtc
     4201 ttgaaaatgc gatagaaaca gtgattcgac actggaatct gcacgaataa aaaaatatca
     4261 aaaaaacttt caataaaggg ttgcaaaaat ctggaaaatt aggtattata actaagtcag
     4321 gttgatacaa acctttcgga ggagtagcga agtggctaaa cgcgacggac tgtaaatccg
     4381 ttccttaggg ttcagtggtt cgaatccact ctcctccatt tcattggggt atagccaagc
     4441 ggtaaggcaa gggactttga ctccctcatg cgttggttcg aatccagcta ccccagtata
     4501 ccacaatcgt aagctctcgt tagttcgaga gctctttttt ttgctttgct accttgttcc
     4561 ttgaagattc tgctgattta agatagagtt aagttaacta caagaattgg agggatccac
     4621 atgaaatgga aagaaaaagt agacgaagca gctgaaaagt tatatgattt ggtcaaatca
     4681 gaaaaataca atattgaagt gaatattccg aaaaaaggcg gaaaagcggt ccaggtaaaa
     4741 tcaaaacgcc caacgaatca tacaaaaaaa tggatggcta aaaaccgcta ataataacaa
     4801 aagatccata agtcggcatt ttcatcgatt ttagggtctt ttgttatttt ctaggttgaa
     4861 catatctaaa aaattgtttc tgacaatagg agtaggaaga acgatgtgtg tctaaatcct
     4921 ctgttgatgg atagaaacct ttatcttttt cagttgtgaa tcaggtatat accagttata
     4981 atagaagaaa cgaaaggacg ggtgtacgtt atgtcgaatc aattacctgt atatatacag
     5041 attcatgacc aattaaaaaa tgaaatcgaa caaggtatat ggaaagtcgg agaccgtctt
     5101 ccttcagaaa gagaattagc agtgaaattc aatgtcagcc gtatgacgtt gagacaagca
     5161 attcaaaact tggcagatga aggaatctta gaaagaaaga tcggttcggg tacatatgtt
     5221 gcgcgagaaa aagtacaaga aaaaatgtct ggtgcaacaa gttttacaga aattatggaa
     5281 tcgcaaaatc gagtaccgtc tagtcggaca atctcttatt tcttgacttc gccaagctca
     5341 agtgaaatgg aaaaactaaa cttgagtaaa gatgacacga tcttgcgaat ggaacgtatt
     5401 cgtttcgcag atgatgtccc tatttgtttt gaagttgcca gtgtgccaca aaagctgatc
     5461 gatggctaca gcaaagcaga gattactgaa tcgttgtatc gcacgctaga agaaaaaggc
     5521 gggaaaaaga tcggtccagc gaatcaaacg attactgctg tgttggcttc tgagaaaatt
     5581 gctgactatt tagaggtaaa acgaggagat gcagttcttc gtttgcgcca gatttcttat
     5641 tttgaagacg gtacgccgtt tgagtatgtg cgtacgcaat acgtaggaaa tcgttttgag
     5701 ttttatttgg aaaaataaaa aacaggtgat ccctgattcc ttacttagaa ataaaagaga
     5761 atcagaatca cctgttttta tatatgatca gttaccagtt gctcgtgata agaaacagga
     5821 ccctcttcaa attggacttg tccgttcagc agctcaatga tggattcttt aaagtggtca
     5881 gcactttttt catctacagc acagatgacg ctaacttgat cagtatattg gatttctttt
     5941 ataaaaaaat gttggagttc aagataattc tgtaattttc ctagttgcgg atacgtgatc
     6001 atcacagaga tctcctgttg caaggtacct tctacgattc caatgtcaga caatgcttga
     6061 gagacagcat gagtgtatgc tcgtattaat ccgcctgtgc cgagtttcgt tcctccaaaa
     6121 tatcgagtga ctactgcagc tacattgatc agttgatttt ttttgaggac ttccaacatc
     6181 ggtacgccag ccgttccact tggttctcca tcatcgctgc ttcgctggat ttcatttttt
     6241 tctccaatga tgaaggcgct gcagttatgg ttcgctttcc aatgttcttt ttttatttct
     6301 tgaatgaaac tttttgcttg gtcctctgat tctattcgtc taagagaaca gataaagcgt
     6361 gattttttga tctcaatctc actttgtcca ttttctttta ttgtacgata gcgctcgatc
     6421 atcttgtcac ctcttttttt cattttcttt attcagtata aataaagttc taggttgttt
     6481 caacactgta acggttgcgc aatcgctgta gtatgcgata ataaagacaa aggtaggtgg
     6541 atctggggat gcaggaacga atgaaaaagt acgatgcaat tacgcattat ttgaagaaca
     6601 atggcggctc tcaagtaacg ttgaccttta cccaatttga cgaactgctg tttcccagta
     6661 acggacttcc caaaacagca agggaaagta cagattggtg ggcaaatgat tataaacacc
     6721 ctgaaaaagg tgcctatggc tggataaatg caggctatga agtagttgtc atcaatttgg
     6781 acaaagaata tgtcgtgttc aataaattag tgaaatctag ttggctattt gattaaataa
     6841 accgcgttta gaaaacgttg acaaatttct ttctttttat ataatagagt aacagacgcg
     6901 aagaaaacgt ttgcgtagtt tgttttaaaa aggagagaaa agtatggcta aaaaaacaat
     6961 catgcttgta tgttcggctg gtatgagcac aagtttacta gtaacaaaaa tgcaaaaagc
     7021 tgctgaagca aaaggaatcg actcggatat tttcgctgtg tccgcttctg acgcagataa
     7081 caatcttgca aataaagatg tagatgtact gttattagga cctcaagttc gctttatgaa
     7141 agctgatttt gaaaaacgtc tggaaccaaa aggcattcct ttagacgtca tcaatatggc
     7201 tgattatggt atgatgaatg gcgaaaaagt gttagatcaa gcaatcacgc tcataggtga
     7261 ataaatagaa cagtataact atcgaacaag cgatccaaaa aatagatcgc ttgttttttc
     7321 gtatattttt ttagggcgaa taatatgaat catttttggg gtagaaggct tctgacgaga
     7381 gatattaaag caatagactg tgaggaggta aatttaccaa attatgagaa gatcgcagca
     7441 gtggaacgtt tgggcaactg gattctttgt catcgctgtg gaatgaggac gtccgtcatt
     7501 gatggacagc ttgcagatta cggttatttt tgtacacatt gtctatattt agggcgatgc
     7561 gacactcaac aagatttata tttgtttgat cagcctgaag caaaacctcg agaagtcgtc
     7621 tttttttgga caggacagct tactgaaaaa caaacggaaa tcgcaaagag aatactttgc
     7681 cattcagaaa aaagacatca tcttatttgg gctgtaacag gtgcagggaa aacagagatg
     7741 ctttatccta ttttagtgga aactttaaaa gctggaggaa gagtagcgat ttgtacaccg
     7801 cgagtcgatg tttgtaatga attgttttta cgctatcgtt atgtttttcc agaagaaaaa
     7861 atcacgttgc tccatgggaa tacagcaacg gcttatacct ttacttcttt tgttatctgt
     7921 acgattcatc agctataccg tttttatcgg tcctttgatt taatagtgat cgatgagatc
     7981 gatgcgtttc cttataacgg ggatgcagga ttagctcatg ctgtgcaaac agcgattaaa
     8041 ccagacggac gattcatcta cttaagtgcc acacctgata aaaagttact aaaggaaatc
     8101 aagcagacgt ttgacatcca caaacttgct ttgcgctttc atcggcgttt acttcctgaa
     8161 cccgtcttgg ttttcatgaa taattggcga caaaaatgca agaacaagaa gaagataaaa
     8221 tctttggtgc ggatcattca tcaattaatt aaagaaaacc atatcctttt attttgtcca
     8281 agtatcacga tgatgcatcg tttgaaagcc acacttcaag aggctcttcc gagatatgag
     8341 atcacagatg tttcttcaag agacaatcag cgggcggaaa aagtccagaa aatgcgagaa
     8401 gcaaaatatg acatactgtt aaccacgatg atccttgaac gtggagtcac atttgaaaga
     8461 atatccgtta tcgttttagg agcggatcat ccggttttta ctaaatcttc tttggtacaa
     8521 atcgcaggaa gagccgatcg taaagggcct tatacgaaca gtcaagtata ctttttctat
     8581 gaagaaaaaa ctgcagctat tcgaaaagct tgtcaggaaa tcaaagaaat gaatgaagaa
     8641 gggaagaaga tgctgtgagg tgcagttgct gcagtaaaga tatcgtccaa aatttaacat
     8701 tcagcgagat tattggtgtt tcgaaataca gattacctca gacttgttca gtttgtcgcg
     8761 cacaatttca tttattggaa acaagaggct gttctggctg tcaaaaacca atccatttca
     8821 atctagagag tacgaaaaag aaagtaaagt ctattcccgt ttactgcagt gactgtcaag
     8881 aatggaagaa gaaatatccg acctatgatt ttcagcaccg tgcctttttt gtgtacgatg
     8941 aaaatatgaa ggcctggctc aatcaataca agtttatggg agatatccga ctagcaggca
     9001 cctttgcata taaatggacg cagataaaaa aagaataccc gacatacatc tattgtccaa
     9061 tacctttatc tgaaaaaagg cgaacagaaa gaggattcaa tcaagtaagc gagatgttga
     9121 aagcagcaag aattcctttt cagatgctac tcaaaagaga gaagcatctg ctggctcaag
     9181 caaaaaacac gcgaaaggaa agattggaga tgcctcaacc ttttgaactt gcagtcacct
     9241 tttctaagat acaaaataaa aaaatattga ttattgatga tgtctataca acaggacaga
     9301 ctcttttcca cgcagcagat tgtttgcagg tcgcacagcc gaaaatcatc cgaaccattt
     9361 ctttagcaag atgaattaga tgtggaaatt tgatagcgat ttaatttgca aataacctag
     9421 agttcgatat aatagaatta agaagaggga aagagtggtc cttctccttc ttgattgtgg
     9481 tagacgaaag gggtaatttt tatgtttaga tataatgtac gcggcgaaaa tatcgaagta
     9541 actgaagcga ttcgcgacta cgttgagaaa aaagtaggca aactagaacg ctatttcagt
     9601 gacgcacccg atgcaacggc gcatgtgaac ttaaaagttt atacagaaaa aacagcaaaa
     9661 gtggaagtaa cgatcccatt accttatctt gtattacgtg ccgaagaaac ttctccagat
     9721 ttatacgcaa gtatcgattt ggttgttgac aaattggaac gccaaatccg caaattcaaa
     9781 acaaaaatta atcgtaaatc ccgtgaaacg ggcatgaata ctgcagatgc tactgcttta
     9841 ttcaatgaag aggacgaaaa cgaaaatgat tctgaattag atatcgtacg tacgaaacgc
     9901 ctttcattga aaccaatgga tagtgaagaa gcagtcttac aaatgaacat gctgggacat
     9961 aacttcttta tttttgaaga tgcagaaaca aatggtacaa gtatcgttta ccgccgtaaa
    10021 gatggaaaat acggactgat tgaaacagac tgatcagcct tacttaaata acaa
//
LOCUS       DAYYCY010000092        10034 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000092 DAYYCY010000000
VERSION     DAYYCY010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10034
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.092"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            217..3444
                     /gene="fss3"
                     /locus_tag="ACHAZB_001852"
     CDS             217..3444
                     /gene="fss3"
                     /locus_tag="ACHAZB_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HFM8614494.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            3557..3871
                     /locus_tag="ACHAZB_001853"
     CDS             3557..3871
                     /locus_tag="ACHAZB_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFM8614495.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            3884..4258
                     /locus_tag="ACHAZB_001854"
     CDS             3884..4258
                     /locus_tag="ACHAZB_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFM8614496.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            4268..4603
                     /locus_tag="ACHAZB_001855"
     CDS             4268..4603
                     /locus_tag="ACHAZB_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HFM8614497.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            4686..6026
                     /locus_tag="ACHAZB_001856"
     CDS             4686..6026
                     /locus_tag="ACHAZB_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFM8614498.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            6104..6778
                     /locus_tag="ACHAZB_001857"
     CDS             6104..6778
                     /locus_tag="ACHAZB_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFM8614499.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            6771..6935
                     /locus_tag="ACHAZB_001858"
     CDS             6771..6935
                     /locus_tag="ACHAZB_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8614500.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            7011..8153
                     /gene="dcm"
                     /locus_tag="ACHAZB_001859"
                     /pseudo
     CDS             7011..8153
                     /gene="dcm"
                     /locus_tag="ACHAZB_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            8143..8433
                     /locus_tag="ACHAZB_001860"
     CDS             8143..8433
                     /locus_tag="ACHAZB_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8614501.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            8692..9876
                     /gene="mobT"
                     /locus_tag="ACHAZB_001861"
     CDS             8692..9876
                     /gene="mobT"
                     /locus_tag="ACHAZB_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFM8614502.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            9873..10010
                     /locus_tag="ACHAZB_001862"
     CDS             9873..10010
                     /locus_tag="ACHAZB_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFM8614503.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
BASE COUNT         3320 a         1683 c         2259 g         2772 t
ORIGIN      
        1 tattttatta tatttgatta aatttgaaat gtttgtgttc taaaatgtgt tctaatttaa
       61 aggcagtata tttcgtattt tagattaaat ttttaggctc attcattctc aaatccattc
      121 tattcagtca ataaaaattt taaaaagact ttataaacaa agatgttcaa gcgagcatct
      181 tatttttttg tgcttaattt gaaaggagaa catttcatgt ataaaaaaca aaaaaagaaa
      241 ttatgtgtat taactatttt gagtttggct gttcaagcaa ttgttggagc aattgctcct
      301 acgattactt ttgctgatga aattacccat ccacaaacgg taaccgtaga actagattta
      361 gcacatcaat atgctgttga aggaactttt agtgatggtc gtccaatgtc agaagtcact
      421 gttccgcatt atgcagtgta caacggcttt aaacaagata ttttctgtat tgaaccagga
      481 gtcccgattt acaatgagtt cactcctggc tatgagaaaa acccgttacc agatatgtcc
      541 gataaagcca aattagtttc cgttttatgg aaaaaagtag gtacagatgc agatactcat
      601 attgtggcac agaagatgat ctggcaagaa gttaatgggt atacccttca ctcaataaaa
      661 cgatcagatg gtagtgccgt aaatatcgca gccattgagg caaaaataaa tcaagccatt
      721 gacgattatc agaagaaacc aaccttccat aatagtacgg ctaaaacagt attagggcaa
      781 tcgactactg tgacggatac gaataatctg aatttgtcag agttcgatga ggttgtggaa
      841 aatgccgcaa acattgatta tcgtgtgaat ggtaatcagt tgataatcac cccaaatgcc
      901 aattcaaaag aaagtggtgt gctgacgctt aagaaatctg ctggtaccgg aacacctgtc
      961 gcctataaaa aggctggcca acagacttta atggccgggg cgattgataa acctaatacc
     1021 tacacaatca aaattgatgt ggaaacggag ggttctttaa agatcaaaaa agtcgataaa
     1081 gaatcgggtg atgttgtacc aggaacggtt ttccatttag actttggaaa aactttacct
     1141 gcaaaagacg tgactactga caaagaaggc attgctacat tggatgagat tcctcatggt
     1201 acaaaagtaa ccattaccga aaaatcagta ccggcgcctt atacgattga taccaccccc
     1261 atgactacca ccattaaagc aggcgaaacc atttacgtga cttcaaaaaa cgcacgggaa
     1321 aaagggcaaa tcattctgga taaaagcgga gttgaaacag ggagtgatct ttggaatgac
     1381 aattattcct tagctggtaa tacgtttgcc attcgcaaag acagtccaac cggtgaaatc
     1441 gttcaagaaa tgaccacgga tgaaaacggt catgcggaaa caccaaaaga gattgctaat
     1501 gcgttggaat tgggaaccta ttatgtgacc gaaactaaag ctagtcatgg gttcgtgaat
     1561 acgttcaaac ctgtaaaagt cgaattgaag tatgccaatc aaaccgtagc ccctgttacc
     1621 agtaacgtaa aagggcaaaa tcaagaagtc actggcgaaa ccactttgac gaaagaagac
     1681 aaagatactg gggataaagc acaaggtaag gcagtctttg agggtgctga atacaccttg
     1741 tttactgcta aggatggaaa agctgttaaa tggagtgagg cttttaaacc agagttagcg
     1801 aagggaacga aaggttctga tgaaacggtc actttgactt tagacgaaaa gaaccaagtt
     1861 gccgttaaac atttagctat caacgagtat tattggcaag aaacaaaagc gcccgaagga
     1921 tatagccttg atgaaacaaa gtatcctgta tccatcaaaa aggtggatga tagcgaaaaa
     1981 aatgccgtga ttacccgaaa tgttacggca aaagaacaag tgattcgctt tggctttgat
     2041 ttctttaaat ttgctggatc agctgcgggc actaccgaaa ctggcttcaa tgatctgacc
     2101 tttaaagtgt cgccattgga agggaccaat gaaatcacag gtgccgaaga tgaagcgacc
     2161 acagcttata acgagcaatt agggtttgat ggttatggca agtttgaaaa tcttccttat
     2221 ggggattatt tacttgaaga agtagaagcc ccagaaggtt ttcaaaagat tacgccatta
     2281 gaaatccgtt ctacttttaa ggaaaacaaa gaagactttg tgaagagtga gtatgtcttt
     2341 accattaccg aacaagacca aaaacaacca attaaaacgg tgaccgttcc ttacgagaag
     2401 ctgacgaaca aagcattttc tgttagtttg aaccgtttaa tgctctatga tttgcctgag
     2461 gaagaagata gtttaacttc tcttgcgact tggaaagacg gaaacaaaga attaacttct
     2521 ctggattcta ccgagctagt tgataaattg agttacaatc tacatgaaat caaagaagac
     2581 tggtatgtgg tagcgcaagc cattgatgtg gatgcgacaa aagctgccca agaaaaagat
     2641 gaaaaagcca aaccagtggt gattgcagaa acaagcgcaa cgttggcgaa caaagagaaa
     2701 acaggaactt ggaaaattca gcataaatta accgccgaac aagttttaaa taaaactatt
     2761 gtcttattca actatgtgta tgaaaataag gaagcttttg aagcaggcga taagccagta
     2821 gcgaaggatg ttagcttgaa taaccaagcc caaactgtca gttgtacagt ggaacaccat
     2881 gtttctatcc aaacaaaggc ccacctagaa aacggttctc agacctttac tcatggtgat
     2941 gtggtggata tgtttgatga tgtgtcgatt actcatgatg tactggatgg ctcaaaagaa
     3001 gctttcgaaa caattctgta tgctttatta ccggatggta caaacaaaga aatttggaaa
     3061 tctggcaaga ttgattatga agtaaatgat aaagaattca ccaaaactgt gcttgcgaaa
     3121 aaagtagata ccggaaaata tccagaagga accaagttta cttttgcgga aatcaattac
     3181 gataaagatg ggaatatcaa tggaaaacac aatgaagatt tgaaagaaaa atctcaaacc
     3241 ttaacaccaa aagaagttcc aaccatacta agtacgccga aacaaccgga aacaccaact
     3301 gttccaagtg gctctcaaga atctagtccc acagtgaaga ccttccctca aaccggggag
     3361 aaaaattcca atgttttact gttcattggt tttactttga tttttgcgac ggcgggctat
     3421 tatttctgga atcgccggaa ctaaggtgat gagatgatac aagtaaatcc taagaaccaa
     3481 cgtgcgcgac cgcctcctaa aaaagtagtc gcgctatttt tgtatccaaa actaacaatg
     3541 aaaagaggaa aagaagatgg aattgaaatt tgtcgtgcca gatatggctg aaacctttgg
     3601 aaagttaagt tatgccggtg aaggagaaat tataacagaa ggatatggac ggaatactac
     3661 ggtgattggt cgcagttatc atttgtattc cagtaagcaa cgggccgatg atattgaggt
     3721 ggtagtagct gcggaagccg gtgaaaagga ttttgatcaa gaccaaccgc taaaagccgt
     3781 gaatccccat ttggttgcca aaggctatga gattgagaat cgtgggttta ctgattacgt
     3841 gttgtatgtt gatgatttag tgaaagcata ggaggaaaag aagatgagat taacagaagg
     3901 tattgtcgta gattcaggtt taacatttgg gaagttgcgt ttttccgcat tacgccgaga
     3961 agtacggaag caaaatgagg atggaacaat tggtaatgag gtaaaagaac gaacctataa
     4021 tttaaaatcc tctgcacaag ggcgaatgat tcaagttagt atcccagcta atgtaccctt
     4081 acgcgagttt gcttatgatg cagaggtaga gttggtcaat ccaatcgtgg ataccgttgc
     4141 caattatgtt tttcgagaag gaactaccgt taattggttc attaaggcgg atgatttagt
     4201 cttgaagcgg cagccgaatc aaggaaatcc tacgaaccaa aatgaaagaa agaaataggt
     4261 gaagattatg gaaattaatg ttgaacatat tttagattgt ctcgatcaat atggaaaagg
     4321 cgagctgact gaggaacaac tcgttaaagc attaacctct gatgaaaaaa tgtttctgat
     4381 tatacatcaa ggtttacttg gagaaaatag cgacagtgaa gaagactctg atgtactaga
     4441 ctggttagga gaagaagagt ctttctttat agttgtcgaa gtaaatgaaa acttatgtcg
     4501 gcaggcagaa tccattttgg aagaaattgg tgtggagatg ccggatgcta ttgaaggctt
     4561 tttaaatcaa ttggttgaaa cgaaacaact cccctcctcg tagcagtaaa tgactagcat
     4621 aactttttga aattgcctaa tgaaatccta ttgggctttt tcgattggag gtggaaacca
     4681 tcaaaatgta caaaggacat cgcatacgcg caggagatca gcacttggtt tatcactttg
     4741 ttcttggctg gctactcgct ctgttcatcg gttggatgag cgtcttttat tttcaagagt
     4801 ttagacaatt tgatatttct aagctatcac tttctactat tgaaatcgtt tggtctataa
     4861 aagatttagt atgtctgctt ggaagtctcg ctttttcagg agctatgata ttgctttata
     4921 ttcacttttt tcttgatcat tggcgaagct tgtggcatcg acaaaagcta gctcggatga
     4981 ttctagagaa ccattggtat gaagtgaaac aaactcaaag tgaaggcttt ttcaaagatt
     5041 tgaatagtag ccgaaccaga gagactatca gttacttccc caaaatctgt tatcgaatga
     5101 aagatggatt actgtctatt cgcgttcaaa tttcacttgg gaaataccaa gatcagcttt
     5161 taaaattgga aaagaagtta gaaagcggac tgtattgtga gttagtagaa aaggaactca
     5221 aagactccta tgtggagtat actttactgt atgatatgat cgccaatcga attgggatag
     5281 acgaagtagt ggcagaaaat gggactctgc gattgatgaa aaatcaagta tgggcttatg
     5341 attctttacc ccatatgtta atcgctggtg gcacgggtgg agggaagacc tatttcctcc
     5401 tcaccattat cgaagcacta ttgaagtcag atgcagaatt gtttatcctc gatcccaaaa
     5461 atgcagattt agctgattta ggtacggtga tgcctcatgt ttattcacaa aaggaagaaa
     5521 tttctgcttg tgtggaagat ttctatgaac gtatgatggc tcgtagcaag gcaatgaaag
     5581 aaatgtctaa ctacaaaaca ggagagaatt atgcctatct tggacttcca cccaactttt
     5641 taatctttga tgaatacgtg gcttatatgg agatgttaac gacgaaagaa agtgcggtga
     5701 ttttgaataa gctcaaacaa atcgtgatgt tgggtcgtca atctggtttc tttctgattt
     5761 tggcttgcca aaggccggat gcgaaatatt taggagacgg aatccgcgat caattcaact
     5821 ttcgcgtggc tttgggtcgt atgagtgaac tcggatattc catgatgttt ggggaggtcg
     5881 aaaaaaactt ctttatgaag cacatcaaag gtcgaggtta tgtggataca ggtggcagtg
     5941 tgattagtga gttttatacg ccacttgtac caaaagggta tgatttctta gattcaatta
     6001 gtaaattaga aaagatgaat gattgagtga tcttcaatac tgactttata cttcaagtaa
     6061 cgtataatac ttttatatag taggaagaaa ggggataatt aatatggaaa gtaatgattc
     6121 ttatttagag tatgcaaaag caaataagga taagtttatt tcttatatta tcaaagggaa
     6181 atcccccgaa gttggacgag acgcagtttt tatggcaggg agtcctgggg ctggaaaatc
     6241 agaggttgcc attggattag ctgagaatta tgataaccat gtgattattg atgcggatta
     6301 ctttcgcacc cagtttcctg attataatgg aaaaaactct agtctctttc aaaaagcatc
     6361 ttcttggttg gtagagcagg catttagata tgtcttagat catggctatt cttttattct
     6421 ggatgcaact tttgcaatat taagtgctga aaaaaatatc tcgcgggcgt tgaaaaaaca
     6481 ttttcgagtg acgattttct atgtttatca agaccctaaa atcgcttgga aatttacaca
     6541 agaacgtgaa attgcagagg gacgtcatgt gccgaaaaag gcatttatta acgctttttt
     6601 caaatcaaga gagaacattg caaaagtaaa gagtaaacat ccagatgtat tgcttcatat
     6661 cataataaaa gattatacga ataatatttc cgaagttcat tatgctacgg ataatctaga
     6721 attattttta ccactagggt atagtaggaa agatttggag gttcaattac atgactgaga
     6781 aagaacaagt aacgaaaatt gtaaagaaat acaataagag cattgcggat ttatcggaaa
     6841 atgctactgc aaaagagttc aaaacggtga ttaaatatgt agccgatcag gccaatgaaa
     6901 aacaacggaa attagttgga ttaaataaaa aatagtagga caaattaata atgtatgagc
     6961 caagagcaaa atgttcttgg cttttttctt tgcaatgaat gaggtaatgt atgagatttt
     7021 tagatttatt cgccggtatt ggtggctttc gtttaggtat ggaacaggcg agccatcatt
     7081 gtatcggttt ttgtgaaatt gataaatttg ctagacgtag ttataaagca attcatgata
     7141 caagtaagga ggtggaaatg catgacatca caagtgtatc agatgagttt attcaatccc
     7201 tcggaccagt ggacatcctt tgcggcggat ttccgtgcca agctttttca attgcgggaa
     7261 agcggcaaag attttccgat actcgaggta ctttattctt tgaaatcgct cggttcacca
     7321 ctcttctccg acctaagctt ttattccttg agaacgtccg aggattgctc aatcacgaag
     7381 gaggggctac gttcgagacg atcctccgaa cgctggatgg attggggtat gacgtggagt
     7441 ggtaggtgct taactcaaag gcctacattc cccaaagccg ggagcggata ttccttatcg
     7501 gacattctcg agacgcatgt accgaacaag tatttcctat cattggaccg tcttcaacat
     7561 ctgatcaaaa catcagaaat ttggtaaata tcaatccttc caatcgagga atgggtggac
     7621 aagtctatgg ttcagatggc gtagcgccaa ccttaacagg tgatgaagga atcaaaatcg
     7681 cactgccagc aagggatgga atctctgtag caggactatt accgggaaag tttgaacaag
     7741 gtaatcgagt ttatgaagtt actggaaccg cccccacctt gtcaacgaaa caaggtggta
     7801 caaaaatcat gattcgaaaa aatgaaacag aatatcaaga ggtgaagcca gaagacagtg
     7861 tgaatctggc ttttcctaat tccacaagtc gccgaggtcg tctgggaaaa cagagtgttc
     7921 ataccttact aacaggagac caacaagcga tcgtcacgga tcagtatcaa attcggaagc
     7981 taacacctag agagtgttgg cgacttcagg gctttcccga ttgggctttt gatcgagatt
     8041 ctcaagtcaa ttcagatagt cagttgtata agcaagccgg aaactctgta acagttccgg
     8101 tcatttttga tattgccaga agattaaaag agttggatgt acatggttta tgatgagttg
     8161 attggagaaa tttattgggt gattggaaag attcagtcag acccagaatt agaagaacaa
     8221 ttgcgacggt taaaatttga tattcgcaag aatggtgtca aagtcgctgg cgatccatat
     8281 ttaatgaatg aggagacgga tgctcgaatt gaaataaatc aagtaattgc agaatttgag
     8341 cggattgcgg accaagcaaa agaaccagat attcgtcaat acttgttaga aatgaaagca
     8401 gagttggaag taaatggtat tacagatgag taacctaaac caacacaact gactgttagg
     8461 cgctgaccgc ggaggattcg cgaagcagaa atcctccgtg gtgctgtgcc tgttcatgga
     8521 cggcgttagc cgaccatgat gtctcacccc cgtatctaac aggggggtac aaaaacacaa
     8581 aaaataagta agcaaccatt aagaatgctt gttgtatcaa agatagtaag taatttgtat
     8641 aggtgttaac tcatttgaaa aagttaacac cttgaatttg gaggtgagaa cttggcacaa
     8701 cggaatttag attatcggct attaaaagac cgacgcaacg aatacggcgt ttcacaaaat
     8761 aaattggcta cggcttgtgg acttagtcgg ccgtacttaa atcagataga aaatggtggc
     8821 gtaaccgcat ctataaaaac catgaggaaa atttttgacc aactggaaag ctttaatccc
     8881 gatttgcctt tgacgttact gtttgactat gtaaggattc gctttcccac aacggatgca
     8941 cgaaaaatta ttcaagaaat tctccatttg aaatttgagt atatgctcca tgaagattat
     9001 gccttttact cctatcaaga acagtatgtc atgggtgata ttgtggtgat gttgtctcat
     9061 gaagaagata aaggtgtgct tttagaatta aaggggcgag gttgccgaca atttgaaact
     9121 tttttactcg ctcaaaagcg tagctggtac gactttttcg aggattgtct aaaggccggt
     9181 ggcgtgatga aacgcttgga cttggcaatc aatgatcgag tggggctatt ggatatacct
     9241 gagctaacga aaaaatgtca gaaggaagaa tgtatctcct tgtttcgtac ctttaaaagt
     9301 tatcgttccg gggaactttt aaaggctgat gaaaaggacg gcatgggcaa taccttatat
     9361 atcggcagtc tcaagagtga ggtttatttc tgtttgtatg aaaaagacta tgaacaatat
     9421 atcaagttag ggattccatt agaccaaact gagacgaaaa atcgttttga gattcgactg
     9481 aaaaatgacc gtgcttttca tgcgattcaa gatttgttaa aaggtcgtag cattgagagc
     9541 accacgtttt ccattatcaa tcgttacttg cgatttgcgg ataaagtaga aggtaaaagg
     9601 cgaaccaact ggcccttgaa tgaacaatgg gggcgtttta ttggacggaa tcggaaagag
     9661 attcaattaa cttccgagcc aaaaccgtat accattgaac gaaccttgaa ttggctaggc
     9721 cgacaagtcg cccctacgtg gaaaatggct aaggagttgg atcgattgaa tcggacaacc
     9781 tatatccagg atatggtaca gaatgcacgc ttatcagacc gacataaaaa gattttggag
     9841 caacaaagta tggcaattga aaatttgatt atatgaaaag aggaatcgtc atgggaattg
     9901 taaaagatat ttttttggta tttggtggca gtatgcttgg tgttactgtg atgtgcttga
     9961 tgaatgcaag tgcagcagcg gatcgagcaa tggaaaagaa aggaaagtga caagatgaat
    10021 ttcggacaaa attt
//
LOCUS       DAYYCY010000093        10033 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000093 DAYYCY010000000
VERSION     DAYYCY010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10033
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.093"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            258..527
                     /gene="rpsN"
                     /locus_tag="ACHAZB_001863"
     CDS             258..527
                     /gene="rpsN"
                     /locus_tag="ACHAZB_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFM8614504.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            542..721
                     /gene="rpmF"
                     /locus_tag="ACHAZB_001864"
     CDS             542..721
                     /gene="rpmF"
                     /locus_tag="ACHAZB_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFM8614505.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            753..1130
                     /locus_tag="ACHAZB_001865"
     CDS             753..1130
                     /locus_tag="ACHAZB_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8614506.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            1151..1300
                     /gene="rpmG"
                     /locus_tag="ACHAZB_001866"
     CDS             1151..1300
                     /gene="rpmG"
                     /locus_tag="ACHAZB_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFM8614507.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            1324..2307
                     /locus_tag="ACHAZB_001867"
     CDS             1324..2307
                     /locus_tag="ACHAZB_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFM8614508.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            2337..2459
                     /locus_tag="ACHAZB_001868"
     CDS             2337..2459
                     /locus_tag="ACHAZB_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFM8614509.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            2477..4000
                     /locus_tag="ACHAZB_001869"
     CDS             2477..4000
                     /locus_tag="ACHAZB_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFM8614510.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            4024..4263
                     /locus_tag="ACHAZB_001870"
     CDS             4024..4263
                     /locus_tag="ACHAZB_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8614511.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(4456..4917)
                     /locus_tag="ACHAZB_001871"
     CDS             complement(4456..4917)
                     /locus_tag="ACHAZB_001871"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM8614512.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(5112..5330)
                     /locus_tag="ACHAZB_001872"
     CDS             complement(5112..5330)
                     /locus_tag="ACHAZB_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8614513.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            5448..7064
                     /locus_tag="ACHAZB_001873"
     CDS             5448..7064
                     /locus_tag="ACHAZB_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFM8614514.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            complement(7305..8645)
                     /locus_tag="ACHAZB_001874"
                     /pseudo
     CDS             complement(7305..8645)
                     /locus_tag="ACHAZB_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(9083..9229)
                     /locus_tag="ACHAZB_001875"
     CDS             complement(9083..9229)
                     /locus_tag="ACHAZB_001875"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8614515.1"
                     /translation="MMDITYIPCNDGRLYLSTYIDLATRILRYYMVDSHMKKEIVVRP
                     LQIY"
     gene            complement(9268..9462)
                     /locus_tag="ACHAZB_001876"
     CDS             complement(9268..9462)
                     /locus_tag="ACHAZB_001876"
                     /inference="COORDINATES: protein motif:HMM:NF024673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM8614516.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKC"
     gene            complement(9372..9704)
                     /locus_tag="ACHAZB_001877"
     CDS             complement(9372..9704)
                     /locus_tag="ACHAZB_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8614517.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            complement(9850..>10033)
                     /locus_tag="ACHAZB_001878"
     CDS             complement(9850..>10033)
                     /locus_tag="ACHAZB_001878"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8614518.1"
                     /translation="FCHSLTPKITLFTYEVGRRSNPELGYGTSTRTSPLELKSMRFTF
                     RNDGRFIQLCVQSKFV"
BASE COUNT         3327 a         1597 c         1991 g         3118 t
ORIGIN      
        1 aaacggtatt tgtgtttgat tggactaata aaagtgaatt cttatttttc agagcctaaa
       61 ctgcatattc ttttaaatga tttgattatc tctatgtacc gataaattcg ttttcaaata
      121 gttatctatt catatgagtt ttatttcatg ttatttattg ttgacaagag caactcttgt
      181 tggtaaagta tcagtgtcaa aacgtaacga ttacgatttg aggtgtctaa atcaaaataa
      241 aaggagtata aaggtatatg gcaaaaaagt cgaaaattgc taaagcaaag aaacaaatgg
      301 cgatgattga aaagtatgca gataaacgtc aggaattaaa agccgcagga gatcgcaccg
      361 ctctcgctaa attgccaaga gattcaaatc cgaatcgtct gaggcttcgt gatcagacgg
      421 atggtcgtcc aagagggtat atgcgaaagt tcggtatgtc ccgtattaag tttagagaac
      481 ttgcccatca agggttgatt cctggagtaa aaaaagcaag ttggtaaaag gaggaaatat
      541 catggcagta ccagcaagaa aaacatctaa ggcgaagaaa cggttaagaa gaactcatca
      601 aactgtagta aaaccagaaa tttcatttga cgaagcgaat ggtgattata gacgtagcca
      661 tcatgtatct ttaaaagggt actataatgg aaagaaagta attaaaggag cttcaaaata
      721 gtttaatata taacttttag gagggaatca aaatggaaat tatttaccca cctttagttg
      781 aagaaggatt aaagtattat cttgaaacga cccagcaatc gctagataaa tgcacttttt
      841 atcgttcgat ggtagagaga ggaattatta ctgaaacggg tttaccgacc caacaggcaa
      901 ttgaaaatgg attagtcaaa gattactatg aggaccaagg cttatcattt gatgattttt
      961 taagaatcta tccaattttt gaagagtatg acgaggagtt atttcaatgt atcgatggtt
     1021 attgggagat acctgttgat atgaaagaaa atttagtttc acaattggaa tctggcgagt
     1081 taacttttga agatgcacaa caaattcaag catatcttga agatcgataa gtattgaagg
     1141 aggaagattt atgcgtcaaa atattatttt ggaatgtgtc gaaactggag aacgtctgta
     1201 tctcaccagc aaaaataaac ggaataaccc tgaacggtta gaattaaaaa agtattcgcc
     1261 aaaattgcgc agacgggcaa tttttaaaga agtgaaatag ctaagtggta aaaaggtgac
     1321 aaaatgggca taccagttac aatcgtttct gggtttttag gggctggaaa aacaacgcta
     1381 atcaatcaag ccttgcaagt ctcaccctac cccagggaag aaattattat aattgaaaat
     1441 gagtttggtg aagttgggat tgatcacaaa ttattagttc atagcgagga acgggtgtta
     1501 caactaaata atggatgtat gtgttgcagt cttcgcagtg atttattagc aatattgatt
     1561 tctttactgg aaacaataga agaacatcaa caaccaattt cacaaatcat cattgaaact
     1621 acaggtatag cagatcccca accaatcatt caaacacttt taacagcgcc tcaactaaga
     1681 ggaagagtat atatcgatag tttattgact gttattgata gtgacagttt tgaacttatt
     1741 gaaagagaac cacaagccct taagcaactt gtcatggcag atcgcatttt tgtgtctaga
     1801 aaacaaaact tgaatttatc tgcactaaaa aaattacaga gagaaatcaa aagtattaac
     1861 cctttatccg atattcgaat gttcacacat agtgaaacga ttcaaaaaaa ggatttttat
     1921 aatttgaaaa aatttaatca tcccttagtt tttgaaagtg attcatcaga aaatcactat
     1981 aatggcaaat actatcatca acaccatcaa gtacatgagt ttaaatcaat tctcttaact
     2041 tcagatcaag acataaaaaa agatttttta ctccaatgga ttgactggct gattttaacc
     2101 aaccaaggga gcctgtatcg ttttaaagga ttgattgctt tacaagagca ggattttata
     2161 gttgccatgc aagaggtaaa cgaacaggtt aattttcaat acaccactcg acaaaaagac
     2221 caaaccacta ttattctaat cggaaaaatg ttggatgaag aaaggatcaa acagagtttt
     2281 gataaattac tggcggaaag caaataatga taagtagtga ttgggggtaa tttctaatgg
     2341 agaaaacgga tatatctagt gcatatcgac gacttaaaag cccgaatatt aaaactagaa
     2401 aaagggcatt aaaaattatc aaagaacata aacgaaataa aatgaagaaa ttagcataat
     2461 taaggaagga aaaagaatga agaaaatcag tgtaggactt attgggatag cagctctagg
     2521 attattggga gcttgctcca gcactaatga tgcaaaagta tcgaatgata aagatggaaa
     2581 attagaaatt gtaacaacat tctatccaat gtatgatttc accaaaaaca tcgtcggcga
     2641 tgaagcgaac gtggatttaa tggttccagc gggatcagaa cctcatgact atgagccctc
     2701 agctaaggat atggcaaaag ctcatgatgc tgatgttttt gtatatcata acgaaaacat
     2761 ggaatcatgg gtaccaaaag ctaaagaaag ttggaagaaa gctgggccta atgtagtaga
     2821 aggaacaaaa gacatgattt tattgccagg tagtgaagaa gaagatcatg atcacggaga
     2881 agaagatcac catcatgaac tagatcctca tacgtgggtt tcacccaaaa tggcaattaa
     2941 ggaagtttca aatatcaagg atcagttagt taaattgtat ccgaaaaaag caaaagtatt
     3001 tgagacaaat gctgaaaagt atttgactaa actgaaacgg ttggatgctg actataccac
     3061 tagtcttaaa gaagcgaaac aaaaaagttt tgtgacgcaa cacgcagcct ttggatattt
     3121 agctttggat tatggattga aacaagttcc tattgctgga ctaagtcctg aagaagagcc
     3181 atcttctggt cgattagctg aattaaaaga gtatgttaaa aaaaataaga tcaattatat
     3241 ttactttgaa aagaatgcta atgataaaat tgcaaaaact ttggccaacg aagctgggat
     3301 aaaattggaa gtactgaatc ctcttgaaag tcttacaaaa gaacaaatgg ataatggtga
     3361 agattatgtt tcagtaatgg aagacaatct aaaagcgtta gaaaaaacaa ctatggttgc
     3421 tggtgaagag gtagttccag aaaaagaagc gaaggatgaa aagacagttg ctaatgggta
     3481 ctttaaagat gccgatgtga aggatcgcga attgagtgat tatacaggcg aatggcaatc
     3541 tgtttatccg ttgttaaaag atggaatttt ggatgaagtt ttcgattaca aagcaaaaat
     3601 caataaagac atgacagctg ccgagtacaa agattattat actactggct ataagacaga
     3661 tattgatact atcaacatta aagacaacac cattgatttt gtagtcaacg gtgaacatca
     3721 tcaatacaca tacaaatatg ttggatacaa aattttgaat tatgaaaaag gaaaccgtgg
     3781 tgttcgtttt aattttgaaa ctgatgatgc aggtgctggt cgttttaaat atattcaatt
     3841 tagtgatcat ggaattgcac caagtaaggc agagcatttc catattttct ttggtggaga
     3901 aagtcaagaa aaactctata atgaattgca taattggcct actttctatc cagcttcatt
     3961 gtctaaacat gaaattgctc aagaaatgat ggctcattaa aattcaattt ttggaggaaa
     4021 tacatggtaa gaaaagtatt attgtctatg gattccaaga atattgatgc ttatcggcgt
     4081 tatcgatttg caaagcaaat gagattaaat gggagtacat tagctgatat tgctgcaact
     4141 ttacatgtta gtattgacac agttcataga gtattaaaac gggatcctga ttgttgtttt
     4201 atcaaggtca atactggaag aagtagacgt ttagcaaaac ggcataatag cttggccaat
     4261 taattgataa taccagagta gatcattttt cccttatttg gatttgatct gctttttatt
     4321 atgtcttgaa ttgtcaaatc aagcgcaaca ctttatcaaa gaagacttca taaggtgttc
     4381 tccaatctaa acactttcta ggtctgttat tcagtttttc aatcatctga tttatcgttg
     4441 agttatctac cgtagctatc tctttcgatt ttggcaagta ttctcgaatg agtctattcg
     4501 tattttaatt tgttcattgt tgccaagggc atgaggatct ggaaaataaa agggcatatt
     4561 attcaatgct tctgtaactg cgaaatgatt ggagaattct tttcctctat ctggagtgat
     4621 cgttttgacc gcctcatttg gaatactgct taaaagttca atcattttct cgctcacata
     4681 agcggctttc tttctgggga ccttctttcc tgctaaaagg aagcatgatt ttctgtctgt
     4741 caaagtcact aaacaagtac caccagtttt gccagctaca atatcggctt tccaatgacc
     4801 aaaatcactt ctatcatctg cggattttgg acggccatgg atcgaatgag taaggcaaat
     4861 ctttccacga gactcgacat gattttttgt atgtcttgtt tttcctcgat ggtgcaatga
     4921 acggatcagg cctttattgc cgtgtgagag gggttctgta tcgaaaagat cattgcaaat
     4981 tgcacgttag atcgtgataa aactaattga aaagtccata tgttcatgct tcaaacgatt
     5041 cgagatttcc tctggcgacc actgaaaatc gacaaataaa tgacgaacaa ttgaccaaac
     5101 tcacggatca tttagaatgt gataacgtcc gcattttttt cttcgataag cataattttt
     5161 ttccgctttg gaaggtgaat agtttttttc ttgtttcttc tcagttcatg taaaatagta
     5221 aacgggctat gctgaatgtc gttgccaatt atactaatag aaaatccttc ttcgtgaagc
     5281 agaaagattc tttctcgttc gagtatagta agatgacgat actggctcat aattattttc
     5341 cttctaggag tgttctcgac aaacttatac tagttggata tcgattatga ctcattttta
     5401 tgtgttgcac ttaaagtgta aattcaaggt atagaaagag gttccctatg aaaaaaatac
     5461 ctattacaat cttaactggc tatcttggtt caggaaaaac gatacttcta aatcatattc
     5521 tgtacggtga tcacggaaag gaaattgctg tcattgtaaa tgacataggg gaagtaaatg
     5581 ttgatggggt attagtcgac aagaaaggtt ttaaaagaac tggagaaaaa cttgtttcga
     5641 tgccgaatgg atgcatttgt tgtacattac gcgaagattt gattgttgaa ctagatcaat
     5701 tagtgcaatt ttctgacttg aatcaaattg ttattgaagc ttcaggaatc agcaaaccaa
     5761 ttccaattat tcaagcaatt atattagcta aaaacccttt agacattgat ttgacccaac
     5821 gattagagat tggtgccatt attatagtag tggatgtaaa acgtttttgg gataatttcg
     5881 aaacgggtga aaagattcag gaaagaaaag ctgaaggtaa tgtagttgaa gaagaacagg
     5941 atattcgtgg cttgttaatt gatcaattcg agtgttgtaa catattactt ttaaataata
     6001 gtgacttagt gtcaaccaag acgatttaca aaattaacgg tctaattgct aagttacagt
     6061 ccaatgccaa aataattagg acaattaaag gtgaagtaga cttatcgaaa attctaaata
     6121 caaatctttt taatatagat gagacttcct attcagtggg ctggttcaaa gagttagagc
     6181 ttggctatca aaatcatatt cctgaaacag aagaatatgg tatctcaagt tttatctatc
     6241 gaaatcggtt actccttgac gcacaaaaat ttgcccatct tacggataaa aattttcctg
     6301 gcaatattac aagagcaaaa gggtttcttt ggtttgccca acatagtgat ttttcgacga
     6361 tctttgaaac atcgggtagc tataaaagta ttgaatcatt aaattattgg gtagctagtt
     6421 tacctaaaat tgagtgtcaa atgattatac aaaaaaatcc cgaattgaag gaagcctggc
     6481 atccgttgtt tggtgaacgc attaataaac tagtttttat tggtatgaag atggatcaac
     6541 aatgtattaa ggaacaactt gagggttgtt taactgattc tcttaatgca gataatttcc
     6601 gaaaaatttc accatttcca tggaaaaagt ctaatatgtt tagccagata tcgttaatga
     6661 aatttttaag aaaagctggt gtcaagatag gcgatgtcat aattattggc attttgatta
     6721 ttacctcttt tattccctta tttgtattct ctatagaaaa ggagaatgaa acaactgaag
     6781 atgctatcaa atatgccatc gttcgaattg atggcaagga aatggatcgc ttcgatttgg
     6841 ataagatcaa tcataaactc gtgaattatc atcctgcgaa gggacaatat aatattgttg
     6901 agattaaaga tggaagaatt cgagttaaag aagataatag tcctgatcaa atcgcggtta
     6961 gaactggatg gatctcaaaa cccggtcaaa cgagtatttg tttaccccat aagttagtta
     7021 ttagtattga gaaaaaagaa agcacagatt actacattta ctaatctgga aatagatgag
     7081 gaggttttta ataagaaaaa gatattatta ttaataatag caggggcttt attaagtgtg
     7141 ttaagcgctt gtggacaaga aaaagaagcg gtgacaacag gcacaagtat ctttaacggg
     7201 acctgcaatg gtttagttaa cagtcggtta gattacttat tttggctctt cgtcaatgag
     7261 tgttggtgaa gaaattcaat agaataatcg agatactcac acggctagac agctatcttg
     7321 tctagccgtg ttgttgttct ggctttagtc agttgggtag tattcttagt tttcttaaaa
     7381 tattttcctc gatataaaaa gattcgttct cggtagtttt gaaagtttct atagccatag
     7441 gcaattcgtt ttagtacttt gatatggtta ttcaaacatt ctatagggcc gttggaatag
     7501 ggaacgcgca acgctcgttt gatttctgtt tggtatttct tgaaggtcgt gaacactggt
     7561 tggtagtgct ctggcaactc cttgtagtct tcttttagga gcgcgacgaa ttcaggaaca
     7621 tcttgtcttc tgataacgga aagaaaatcc tggtagactt cgtagcctcg ctttaaggaa
     7681 tcatcataag ataatagccg atcgactatc tctgcttctg tcagatgatc tcgaaatgac
     7741 ggacgccagt agcgatgctg acctttcaat tcccacgcat tcttttgaag tagtttccag
     7801 tatttcttta agtgcttcga ccgacgatcc ccttttttaa aggtcttcat tacttgaatt
     7861 cggtggttgg taaaagcccg accaatgtgt tggctaatat gaaagcgatc ggcgacgatt
     7921 cgggcattcg gaaaacactc tttcaccaat gtgcgatagg gtgtatagaa atctgaaacc
     7981 actagacgca cttgttcacg gacttttata gggtagcgca agaaatagtc gcgtaaagga
     8041 ttgagttgcc gattttccac aatatcaatc agttgatggg tcttgccgtc catcatgaca
     8101 aagctcatgg ctcccgagac atttttcact gatttgaatt catcgaaaca caccacttct
     8161 ggtaacagct ggctcaaaga aatggtttta ggccgaaggc tgcgaagtat tcgaatgaca
     8221 gtggtcggag aaatgtgctt catacgtgcg atcaaagaca tagaaagagg ttcgcttaat
     8281 agctcaagaa tggcttgttt gactctgcga gcaatggagc agcgacgatc actaactgaa
     8341 tcttctgcta aaaaagtctt tccacagtgt ttacatttga agcgctgttt gttaatccgc
     8401 agaatggtct tgtagttact gacatcattc aataagatgg atactttttt ccagccctat
     8461 ttaatgatcg tttgttcatt ttgatagtgg cgatgaaagc attcctccgg ttgataggtc
     8521 aatgtgccat caaaaacaaa acatgtttcg tcattgattc gctctttgcg aaaggcatct
     8581 tcatgaaaga taagatttag gtctagaata tctagtaact ctttggtaaa ctctgaatag
     8641 gacatcttga aacactcctt tgaatggttt tcgtcgactt tattctaaag gattttaagg
     8701 tgtctgtttg cgtttattag aaaaaagaaa aagtattggc aacagaaggc gtcaccaaca
     8761 ctagatatta tagatccctt attttcttcg cttcaaattg tcaaggagtc atcataaatt
     8821 ttgtatggat tcttttgttt gggtagtagt cttctaaata atcttggatt aacaccttca
     8881 tctctatttt acttttatat ttgttggagt cgattaattc tcatttgatt gatcgatgaa
     8941 acgcttcaat tactgcattg tcaacaggag tcccagtttg gctcactgaa tgtgtgatgt
     9001 ggtggtcgtt caacagctct tgataaataa aagaccgatg ctggctacct ctatcggaat
     9061 gaattaccgt aggcaattct ccttaataaa tttgtaatgg acggacgaca atctcttttt
     9121 tcatgtgtga atctaccata tagtaacgta gaatacgagt tgctaagtca atataggtgg
     9181 acaaatagag gcgaccgtca ttacatggaa tgtaagtaat atccatcacc caaacagcat
     9241 caatttggtt ctgttcaaat tatcgattca acattttttt accatttctt tgattgtttt
     9301 ttctctacca tgagaagatt tgcgtcgatg aaatcctctt gtgcataaat tcatttcacg
     9361 cataagaaca gctacaacac gtttattgat ttcgattcct tcatcgtaaa gtttttgagt
     9421 gatccctgga gatccgtatc gatctttgtg ttcgtagaac actcgtcttc ctcttttagt
     9481 cgtttatttt cctgttcaag aatgtagaaa tctgctttat caggtaatct ttttccttgt
     9541 ccagcaaaag catttttccc gtatcgacga tattctgaaa cccatcggga aattgttgga
     9601 cgagagatat tgtattcttc agcaattttc ttggtagtta cttttttctc aaggatatcc
     9661 tgtgaaatct ttagtttaaa tttacgatca tatgtttttc tcaatagagt tcattccttc
     9721 cggtaacgta actgatagtc aagaaaagtg tatcatagcc caacttagta tcagggtttg
     9781 tcaactaaac tgtggaagtt gatagataag agtttttaac aaccaaaatt ctcttgacta
     9841 tttttgaaat cagacaaatt ttgattggac acatagttga ataaatctac cgtcgttacg
     9901 gaaggtaaat cgcatgcttt tcaattctag aggagaagtc ctcgttgaag taccatagcc
     9961 cagttctgga ttcgacctcc tgcccacttc atacgtaaat aaagtaattt taggagtgag
    10021 actgtgacaa aag
//
LOCUS       DAYYCY010000094        10001 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000094 DAYYCY010000000
VERSION     DAYYCY010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.094"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(221..2341)
                     /locus_tag="ACHAZB_001879"
     CDS             complement(221..2341)
                     /locus_tag="ACHAZB_001879"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFM8614519.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2571..3749
                     /locus_tag="ACHAZB_001880"
     CDS             2571..3749
                     /locus_tag="ACHAZB_001880"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HFM8614520.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3765..4523
                     /gene="aroD"
                     /locus_tag="ACHAZB_001881"
     CDS             3765..4523
                     /gene="aroD"
                     /locus_tag="ACHAZB_001881"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFM8614521.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4546..5031
                     /gene="ybaK"
                     /locus_tag="ACHAZB_001882"
     CDS             4546..5031
                     /gene="ybaK"
                     /locus_tag="ACHAZB_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HFM8614522.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(5102..6355)
                     /locus_tag="ACHAZB_001883"
     CDS             complement(5102..6355)
                     /locus_tag="ACHAZB_001883"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFM8614523.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6473..7822
                     /gene="gdhA"
                     /locus_tag="ACHAZB_001884"
     CDS             6473..7822
                     /gene="gdhA"
                     /locus_tag="ACHAZB_001884"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFM8614524.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7934..9280
                     /locus_tag="ACHAZB_001885"
     CDS             7934..9280
                     /locus_tag="ACHAZB_001885"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFM8614525.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9588..9974)
                     /locus_tag="ACHAZB_001886"
     CDS             complement(9588..9974)
                     /locus_tag="ACHAZB_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFM8614526.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         3204 a         1806 c         2045 g         2946 t
ORIGIN      
        1 ttttcacctc tataaaaagt atagaaaatg aatatgaata aagtatgaaa ttcctttagt
       61 gaaatcacga attaaaacca gaaaatattt tgtaaaaagc aagaggttgt gaagaagctg
      121 ccctattcta ttaccgtaga tgcaagcttt tgaacactgt ttattcgaaa agaggagcag
      181 cgacaataat tttgtcgctg ctccttttaa atttattttg tcattcggaa gaacttgtgg
      241 tcgttccgtg aagttcttgg tacgtttgcg ttttataaag atcaacagtt gattgtccgt
      301 tgtgttcact aaagacacta gttgacttgt caccaagaga tttttcagct tcttttaatt
      361 tctctaagcc attcttgtag tcataatcac tggtatcaat tgggtctaat ccactattcg
      421 tatagaaccg aagaagatca ccattattga tttggtcaga aacagctaac tgtttatcaa
      481 ctttttcttt cagtgaatca atttcattct gtacttcctc agtaggttca gtaatagctt
      541 caccagtttc gttactatag atcgtaccac cgtagtaagt atattttggt gtaacaaaat
      601 caccatcacg gaaagaaaca agctgttcat gatctttcga gaatagatct tgtcctaact
      661 gaatataatt ttttgtatct acacctagta agtgaagtaa tgtaggcaag gcatcaactt
      721 gtccgccgta agtgtgattg attccgccct gtgtttgtcc agggatatgg atcatataag
      781 ggacacgctg catttgtgca ttgtcgtagt ttgtccaagt agaacttgtt tttccaacta
      841 attcagctag atttttattt cttgaattcg agacaccgta atggtcaccg taaaggacga
      901 tgactgaatt atcatacaag ccgctttctt tcaaataatt gaagaattct tgtacggcgg
      961 tatctaaata attggctgtc gcaaagtaac cgttgatcgt ttcatcattt gtattggcta
     1021 acgggaatcc tgcttcgtca ttggtgaatt ttgaataagg ataatggtta gatacagcaa
     1081 tgaattttga ataaaatggt tgttgcagac gttccaagta ttgtacggat tgttggaaaa
     1141 atggtttatc atgcaacccg tattggaacg aattgtcatc attaacatca taataagaag
     1201 aatcaaagaa ataatcatat cctaaattct tgtatgtctc gtttcgattc cagaatgtac
     1261 cagcgttccc atggaatacc gcactagtgt aaccttgtgt ttgacttaaa atatcaggtg
     1321 cagcttggaa agtatttttt ccgcctaatt gtgtgaacaa ggagccttga ttcaagccaa
     1381 acaatgaatt ttccatcagc gtttccgcat cgcttgtttt tcctgctttt acttgatgga
     1441 aaaagttgtc aaaactgaac gtgctgttgc tgtggtaaag actattgatg aaaggtgtaa
     1501 cttcatgctg gagaccattc tcatctgtca atttataatc gatcaggaat tgctggaagc
     1561 tttctaaatg gatataaatc acattttttc ctttagcgag cccgaacaaa tcgtcatttg
     1621 gtgctgcata atgttctttt acataatttt ctacttcttt catatcattg ggacttgctt
     1681 ctgcccgcac ttggtttgtc tggtaagttt gaacaccgtc ataaaccatg aaagcattca
     1741 agccaagata tttgaccaaa taatcacgag agaatgttcg agtcagcaat tctggacgat
     1801 cagcttccgc cataaaaaga tttcctgaga aaatcataac tgctaatgta gagatcgcaa
     1861 aagccattct tgctcggatc gggcgagggt ccaacttgat tttttttgca atcagcagaa
     1921 cagtcaagat aataaaatcg atccaataga tcacatcata tgggcgaaac agtcgaatgg
     1981 cactttctcc cagaccactc gctactttcc cagcacctaa aatcgtgttg atcgtaatga
     2041 agtccgtgaa ttctcgataa taagtgacat tcgaaaaaac aagcaatgtc aaaagaaaat
     2101 aaatgaccat catcgtgata taagcaactc tagggcgtct gatataaaga gcaattccaa
     2161 gtaataacaa tgtagttgca atagggttga taaacaagat aaagtattga atgccacttt
     2221 cgattccgag atggaaatca actaaatagg caaaaatcgt tttcaaccaa agtaaaacga
     2281 ctagtaaaga aaaaaatccg agcctcttat tcaaaaaggc aggtgttttt atacatttca
     2341 agataatctt cctttctgca cttcttaatc acagaagtgt catgaaacca tgttacacag
     2401 ctcacttatt ctcatttatt ttactagtat gacaatatag tgtcaattga aaccagaagt
     2461 ttatgctgtt tcattaaggt tttctttaag cttcatgaaa aaatgagcag taagacaagt
     2521 cctatgtttt ttgttatact tgggaagaga aagctaaagc aggtgaagga atgaaagtaa
     2581 aagtaaaaaa aagagcaagc aatcgattga aaaaaggata tccactgatc caaaaagaag
     2641 accttgctca tccccaaaaa gaaaccacgc aatgggtgga attagttgat tcgcaaaacc
     2701 aatttatcgc aaaaggatat ctaggaaagc agaataaggg aatcggttgg gtattgagcc
     2761 agcagaacat cccatttgat caatcttttt ttgaagaatt ttttgtgaag tcaaaagaaa
     2821 aaagaacttt ctttttttca gatgattcta ctaccgcttt tcgtatattc aatggagaag
     2881 gagacggaat cggcggtctg attgttgatt attatgcagg ttatgctgtt ttttcttggt
     2941 ataacgaaac gttatatgca catcgtgaat tattgattca agcttttcgt gaaatctacc
     3001 cggaaatcgt aggaggctat gagaaaattc gatttgaatc agtaaatctg cctgagagcc
     3061 agttcattta cggaaaagag gcaccggaac ccttgatcgt aaaagaaaac ggagtaaaat
     3121 ttgccgttta tttaaatgaa ggattaatga ctgggatctt tttagaccaa aaagaagtac
     3181 ggggatcatt agcagaaggt ttcgcttctg ggaaaacagt tttgaacatg tttagttata
     3241 ccggcgcctt ttctgttgct gccgcaatgg gaggggcaat cgaaacaaca agtgtcgatc
     3301 tagcaaaacg cagtttaaaa aaaacacagg aacagtttga agtgaatggt ttggatgtcg
     3361 atcagcaaaa aatcattgtt atggacgtat tcgagtattt caaatacgcc aaaagaaaac
     3421 aatttttata tgacatgatt gtattggatc caccaagttt tgccagaaat aagaaaaaag
     3481 tctttcgcgt agcaaaagat tatggggaac tggtcaagga tagtctggat attttagcag
     3541 aagacgggat acttattgcg tctacaaatg ctgcgaatgt ctcgatagaa caattccaaa
     3601 caatgattga agaggaattc catgccaaaa atgtttcttt tgaacaactt gctttatatc
     3661 gtctgccaaa agatttccag acgacggata cctttttaga aggcaattat ctgaaagtgt
     3721 ttgtttatca aattaaaaat aaccattaga aggagcgatg cagaatggtt tgggtaaagg
     3781 gattgaatat aggaagcggt agaccaaaga tttgtgcgcc tcttatcggg gccgtccgag
     3841 aggaagtatt acgtgaagca gttcttgcac gtgaagcagg ggccgattta gtcgaatggc
     3901 gagtcgatca ttatcgtgaa gttttttctt ttgatgaagt agcagaaaca ttggttctta
     3961 tccatgagat gcttgatgga ctgccgttat tatttacgtt tcgtacctta gctgaaggcg
     4021 gagaacaaga catatcgatc cgtcagtatc gcgaccttta tgaacatgtg atacacacag
     4081 gattagtaga tatggtcgat atcgagttgt tcaaagtaga aagtctagga aaaagactga
     4141 tagaggaaat caagtcttta aacattcctt taattatcag tagtcatgat tttaaagaaa
     4201 caccagcaga ccctgtcttg ctttaccgat tgaatgttat ggaacatttt ggagccgata
     4261 tcggaaaact ggcagtaact cccaataatg aacgagatgt tttgcggttg atggagttga
     4321 ctagacgggc aaatgctttt gtctccatgc ctattattac gatgtccatg ggaaatcttg
     4381 gtaaaatttc tcgtctggct ggcggaacga cgggttccgt tatgacattt ggttccttaa
     4441 gcagcgattc tacttctgct ccaggacaat taccggtcaa agcattgaag caaattattc
     4501 aattattaaa tgaaccgttg tagaacaagt ttgaggtgat attttttggc aaagaaaaaa
     4561 atagtaaaaa cgaatgcaat ccgtatggtc gaacaaaaaa agattcccta tacagagcac
     4621 gaatatgagt gggacgaaag ccacctaagt gcgtcatcgg tcgctgaaca gcttccagaa
     4681 agccaatctc gtatatttaa aacactggtt gctgtgggca atgtgacggg gcctcttgtt
     4741 gctgtgatac cgggagaagc cgaattgaat ctaaaaaaat tagcaaaagt ttcagggaat
     4801 aaaaaagtag aaatgctcca tttaaaagat ttggaagcaa cgaccggata catacgtggt
     4861 ggctgctcgc ctataggtat gaaaaaacta tttcctactt atttagatca aattgcagaa
     4921 agctatgaac aaatcattgt ctcggctggc agaagagggc tacagatgga gttagcacct
     4981 caagatatct gtgcattaac gagcggacaa tttgcagata taaaacaata aatcatacgt
     5041 ataaaagaaa gaaggaggcc gggatgttgt ttgtccagtc tccttctttc ttttttatac
     5101 attatgctct ttgcccggca ttcattcctg ctacatgccc tgtagcgaaa gccgctgtga
     5161 tattaaagcc gccagtataa ccattgacat ccagtaattc tcccgcaaaa tacagaccat
     5221 caatgatttt actctccaat gttttaggat aaatttcctt tagagaaacc cctccgccag
     5281 ttacaaatga tttctcaata ggaaaagttt tgttgattga tagcttaaac tctttgcata
     5341 gttgaacaaa atcttgaatt tgtttttgag aggcttgttg tcctgtcaga tgatccattt
     5401 caagccgctc aagaaagaaa actaacaatc gctcaggcaa tataccatgc caagcattga
     5461 ccaagttctt tttggttgct ttactttttt ctgtcaatat atgaataagc tcttgtttgg
     5521 tttgtgtagg gaaacaatcc agagaaagtg tcactggttc tcccgttttc tctaattctt
     5581 tattgacaaa acttgaacaa cgcagtgcag ccggaccaga aataccgaaa tgggtgaaaa
     5641 gtaaatccat cgtatgttca gtcaatacac gacctttttt atttaaaacg cgtaaagtga
     5701 tgtcttgtaa agaaagccct tgaagtgttt tctcctgaat atacggttca tccgatatca
     5761 agggggactc tgtcgcataa agaggcgtca ccgtgtgacc cactcgcttc acgatcttgt
     5821 acccatctcc agtagaacca gtagatggat aagtccgtcc accagttgtt aagataatgg
     5881 caggagcatg gaactcttcg aattccgtgc gtataccgta aattttatgt tctttgtgga
     5941 tcaatttctc tactcgagta gagaatagaa gcgtcacatc tagttctttc agacgattga
     6001 gcaatgcttc gataatcgtt tttgatttat ttgttacagg aaacatccgt ccatgatcct
     6061 cttcttttaa atgtacgcct tgtgattcaa agaagttcat gatatcgaaa ttattccatt
     6121 gcgcaaacgt actatataaa aactttccat ttccaggaat atgtgcaatc aaatcatcaa
     6181 caggacgatt gttcgtaaca ttgcaccgac caccacctgt catcagtagt ttttttcctg
     6241 cttttttgtt tttttcgatc aagagtacgc gtgcgccttg ttcagctgca ctgatcgctg
     6301 ccatcatccc gcttgttcca gcgccgacta cgatcacatc atacagtttt tccattccct
     6361 cacctcaagg aaagtgtagc atatctgaag aaagaaaaca gaaaaaaaat gttttgagcc
     6421 aaaagagaaa atgagctaaa atgagggaag ataagcaaag gagcgtggaa cgatgacaaa
     6481 cgcaacagaa tatgttcaag caattcaaga aaagctacat caaaaggatc aagggcaaga
     6541 agaattttta caagcaatcg atgaattcat gcccacggtt atgtcttttt tggacactca
     6601 tcccgaatat atcgaaaaga atatcttaga acttttaacg gaaccagaaa gagttattca
     6661 atttcgagtg ccttggcaag atgatgaagg aaactggcga gtaaatagag ggtaccgtat
     6721 ccaatacaac tcagctatcg gcccttataa aggtggattg cgcttccatc ctagtgtcaa
     6781 tctaagtatc ttgaaatttt tggcttttga acagattttc aagaatagtt taaccggtct
     6841 gccaatcggt ggaggaaaag gcggaagtga cttcgatcct aaaggaaaat ctgataatga
     6901 gatcatgcgt ttttgtcaaa gtttcatgct ggaattgtca aaacatatcg gcccatcgat
     6961 tgatgttcct gcgggagata tcggtgtagg agctagagaa atcggctatt tgttcggcga
     7021 atataaacga ttgaaaaaat atgatactgg tgtgttgaca ggtaaacctt tggatttttg
     7081 gggaagtaag atacgtacgg aagctactgg ttatggatta gtgtattatg tcaaacatct
     7141 attgaacgaa gaaaaagatt catttaatca aaaaacagtt tttgtttccg gaagcgggaa
     7201 tgtagccatt tatgcaatcg aaaaagtgca agagctaggc gggaaagttg ttacatgttc
     7261 agattctagc gggtacatct atgatccaga aggaatagat gtgaaactat tgaaagaagt
     7321 caaagaaatc aatagacaac gtttgacgga atacgcaaac caacgtcctt ctgctattta
     7381 tcatgctgga gaatctgttt ggacattgaa agaaaaagcg gatatcgctt tgccatgtgc
     7441 tactcaaaat gaaatagacg aaagcttggc cgaaatctta gtagaaaatg ggatcaaaat
     7501 cgtagcagaa ggcgctaata tgccatgtac agtaaaagct gtttctgttc ttcatgaagc
     7561 aggcgtgtgg tattgtccag gaaaagctgc taatgctgga ggagtagcag tatctgcttt
     7621 agaaatgagt cagaacgtcc agcgattgac ttgggaaaga gagcaagtgg atgagcagct
     7681 ggatcaaatc atggaaacga tttaccatac gtgtcgccaa acagcagccg agtacggcga
     7741 cgaaaaaaat ctgcttcttg gagcaaatgt tgctggattt gaaaaagttg ccaaagcaat
     7801 ggcagcagaa ggtttagttt gaaaaatgaa actttcttgg aaatattgtc agcattttgt
     7861 ttatttgctt gaaaaataat aaaaaaaaac gtaaagtatt acctgtagag taaacctata
     7921 aggaggagaa caaatggcac acattcattt tgactattca aaagtagcac cgtttgtcaa
     7981 cgaacaagag ttagggtaca tgcaaagcca agtaacagca gcccacaacg aattacggga
     8041 aggtactggc gcaggaagcg acttccgcgg ctggatcgat ttaccaacga actatgacaa
     8101 agaagaattt gcacgtatca aagaagcagc taaaaaaatc caatctgatt ctgaagtatt
     8161 agttgttatc ggtatcggtg gttcttattt aggcgctcgt gcagcgatcg acttcttgaa
     8221 tcacacattc ttcaatttat tagatagcaa cacaagaaaa gcaccacaaa tcttctttgc
     8281 cggaaattca atcagctcaa catatattgc tgacttgatc gaagtaatcg gtgatcgcga
     8341 cttttcagta aacgttattt caaaatctgg aacaactact gaaccagcga ttgcgttccg
     8401 tgtattcaaa gagcttcttg ttaagaaata tggacaagaa gaagcaaaca aacgtatcta
     8461 cgctacaaca gataaagcaa aaggtgcagt taaagtagaa gcagatgcag aaggttggga
     8521 aactttcgtg attcctgatg acatcggcgg acgtttctct gtattaactg ctgttggttt
     8581 gttaccaatc gctgttagtg gtgcagatat cgatgcattg atgcaaggtg ccgctgatgc
     8641 aagcaaagca tactcaagcg acaaactaga agaaaacgaa gcttaccaat acgcagcaat
     8701 gcgcaacatt ctttaccgta aaggcaaagt aactgagttg ttgatcaact atgaaccagg
     8761 aatgcaatac ttctcagaat ggtggaaaca attgtatggt gaatctgaag gaaaagacca
     8821 aaaaggcatc tatccttcaa gcgcaaactt ctcaactgat ttgcactctt taggacaatt
     8881 catccaagaa ggacgtcgca atatttttga aacagttgtg aaggttgaaa aacctcgtaa
     8941 atcaatcaca attcctgaac aagcagaaga tttagacggt ctaggctact tgcaaggaaa
     9001 agaagtggac ttcgtaaata caaaagcatt tgaaggaaca ttgcttgctc atacagacgg
     9061 tgacgtgcct aacttgctag tcaaaattcc agaaatggat gcttatacat tgggttatac
     9121 aatgtacttc tttgaaattg ctgtaggtat ttcaggttac ttgaatggaa tcaacccatt
     9181 cgaccaacca ggtgtagaag cttacaagaa aaacatgttc gcacttctag gcaaaccagg
     9241 ctttgaagat cttgctaaag aattgaacga acgcctgtaa gataagggtt tttcgctaga
     9301 tttaaataat cagtgataga aacatgttcg ctttaaaatg aaaactggta cccatttagg
     9361 tactcaaaca tactgcatcg tttattcaga ataaggggct gtgacaagac gtttgtcaca
     9421 gtcccttgtt tgtatagaaa tcgattgatt gccttgctct ttagtttgtt ttaggacttt
     9481 aagaacagtg aggctgactt cgaaggttaa ttctgttttt ttgttttcta gaatcagtaa
     9541 gcaaaaaagc actcacttcg agtgcttttt tgcgatatat tcttagctta agataggcct
     9601 aatttttctt ttacagatga gggtacctta tcgctggtaa ttactttttt ctctatgatc
     9661 cgcttttcgc ttgctttcaa ctgcaagatc gttccttcgg ggtaagcaga accattggca
     9721 gagtcaccag aagtaatgac atcgaaatct aactgtcttt tttcaccgtt ttcagcataa
     9781 gcatctacgt tgtatttaaa acctcttcca attacttccc cattatctgc tttgatatca
     9841 gtttctgcag gcattggagc tttgactacc gcataataat ctttacctac ataagtagac
     9901 tgatagtagt cccaaccttt ccatcctata aaagcaatca atatgataac gatcaaccct
     9961 aaaatttttt tcatgaaaga tctccttttt tatttttatt a
//
LOCUS       DAYYCY010000095         9975 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000095 DAYYCY010000000
VERSION     DAYYCY010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9975
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.095"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            241..720
                     /locus_tag="ACHAZB_001887"
     CDS             241..720
                     /locus_tag="ACHAZB_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="HFM8614527.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            753..2981
                     /locus_tag="ACHAZB_001888"
     CDS             753..2981
                     /locus_tag="ACHAZB_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8614528.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            3110..4753
                     /locus_tag="ACHAZB_001889"
     CDS             3110..4753
                     /locus_tag="ACHAZB_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFM8614529.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(4826..5371)
                     /locus_tag="ACHAZB_001890"
     CDS             complement(4826..5371)
                     /locus_tag="ACHAZB_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HFM8614530.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(5394..5879)
                     /locus_tag="ACHAZB_001891"
     CDS             complement(5394..5879)
                     /locus_tag="ACHAZB_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFM8614531.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(5869..6681)
                     /locus_tag="ACHAZB_001892"
     CDS             complement(5869..6681)
                     /locus_tag="ACHAZB_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HFM8614532.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(6820..8352)
                     /locus_tag="ACHAZB_001893"
     CDS             complement(6820..8352)
                     /locus_tag="ACHAZB_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFM8614533.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            complement(8461..9492)
                     /locus_tag="ACHAZB_001894"
     CDS             complement(8461..9492)
                     /locus_tag="ACHAZB_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8614534.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT         3318 a         1844 c         1919 g         2894 t
ORIGIN      
        1 tggtgaagtt caccaacatc aaaaagtata gagccaaaat attccaacat catcttggac
       61 tttctacatg ttactaatga gcgtgtaaaa gaacgagatg gtgtttttat ttctatcaat
      121 gaagctcttt ttctttgcaa tcctcacttc aatcctctat aatgtcgcat atagtttagt
      181 aaatgagtaa actactaatt tacttattta ctaaaaataa aaggatgagg agttaaaata
      241 atggaaatac caacaaattt aaaacataaa ccagtaatcg ttgtagaaga ctatgataaa
      301 gttgatggaa gaaatgcatt aaatatggat gcaaaagggc tttcactggg ccttgctcag
      361 tggaatgatc gtggaaaagt cgatatctca ggcaaggtat ggcgacatac tggagaaaaa
      421 tggtcacgac agtcggaaga attaccaatc acacgtattt tagatttagc gatcttagct
      481 gcgcaaggaa gtttatactt ccaagatgct tatcgttatg aaaaatttta tgatccagaa
      541 aatccagtcg tagacatcat tggattacaa gggaaccgaa tgacggtaga agtagatacc
      601 aaaaatccaa agatcgatga agacatcatt ttgtattacg atacactaca aaaagacgga
      661 gaactgattg gggaacgatt ccgtatttta aaacgtcttt tagatgagct aggatattaa
      721 tagacagaga tgatcaaaag gggaagagaa gcatggagaa tttctcacta acagaaaacc
      781 gaaccttttc acggctagaa aagcaaatga tctggaagaa accgaaaagc catcggacaa
      841 gtgcagaaga atggcggatc gcctccgaaa tcaaagggaa ttggcaagat cctgaaatga
      901 aaatcagtaa cgtacttttg gaaggagatg ccgggtcggg gaagacacaa ttagccaaag
      961 cactatctgc tgatctgcaa cttccctata caaaagtgac ctgttttgca gatatggaca
     1021 aatcagatgt tttcggagcc ttattacctg tagtagattc taataaggaa caagaccaag
     1081 aattactaga agctatttac caaacagata cacttgaagc tgtactgaca ctgatccaga
     1141 accattacgg cattgatcct ttttctgcta aagaaaaatt agcagagcta gttcaaagga
     1201 tcgaagctga ttatccagat gcaatccatt acagctatta cccttctgaa atcgttcgag
     1261 cgattgaaaa aggctattta ttagagattc aagagccgac tgtcatccga gatgcctctg
     1321 ttcttgtggc acttaactca gcgctcgaac caaatgggat gttgaattta ccgacaggaa
     1381 tcattcgtcg ccatccagat tgcgtcattg tgatcactac aaatcgaaat taccaaggaa
     1441 atcgcccatt aaatgaatct ttaagagatc ggatgcaaca tgcggaaaaa atggatcttc
     1501 ctgccttaga agtcatggca gaacgtgcga tggcgaaaac tatgatccaa aaacaagaac
     1561 ttctcttaaa gatggcagag attatccgtt tgttggatga aactgcaaaa gctaacgcca
     1621 tcaaaggtgt agcaggaatg cgttcttatt tttattgggc aaatacctat aagcaaggac
     1681 aagatttact ccagtcgatt tatccaaaag ttttgtataa attatccact gattcagatg
     1741 aattggcaat tcttgaacaa gctttgtctg acagtggatt gctactggaa cttcaggaca
     1801 tattgagaaa agaaaaatgg ggtcatcctc aatctgagca tacaaaagaa agaaccatta
     1861 gcgccgaaga agcaaatgaa cgaaacatca ctgaaaatgc tgaagaaatt ctgcgcttag
     1921 atgaaaagaa atcgttgtct gctgaagaac caaatgaggg agaagagaaa caagaagaaa
     1981 caatagaaaa aaacgagatt cctcctcaaa tgcaaaaaga acgcagtagc gatatcgaag
     2041 gaaaaaatca aaaagaacaa caaacaggag atgattctca agaaatgtct gcttctgaca
     2101 tggaagcaca tgataaggaa atcaagaagc aactgaataa agaagcgcga caaatcatga
     2161 aggaaacgat tcatgaaaaa gaaggtctca tcgttcaccg accagctttc caaggaaaaa
     2221 cgccggaagc acgtgagcta cacatgcaag taatgccaat agtagaatca cttagccggc
     2281 aaattttaga gttactggat aatgaacaaa cagagacgta ccaaaaagga aaatacgaag
     2341 gtcagcggtt caatgcaagc aaagtagcat atggtgattt gagaaatttt gacaaaaaga
     2401 acccgccaca tgagcaacct tctttagcgg tagctgtacg gatcgatgaa tctgggtcaa
     2461 tgatacggga tgatcggatc gaagcggcaa aaaaagcagc gattgccatt gccgaatttt
     2521 ctaagaaagt cgatatccca ttgttgattt acggagatac agcagaccgt tcgtcaagag
     2581 aaaagacttc tttgttttct tataaagaat ttgaagacgg atttcaatgg ttaaatgaaa
     2641 aattagtcac tatgaaacca aggcaaaaca atcgtgatgg tgctgcactt cgtttagcag
     2701 cagaaaaatt aaacagacaa tccgcaacaa ccaaactgtt gcttaatatt agtgacggtc
     2761 agccaaaagc attgccggat tatacaggca aaaaagcaaa agaagatatc cagtctgttt
     2821 tgaaagaata tgaacgtcaa ggaatcctat ttgtctccgc agctattggc caagacaaag
     2881 aagaaatcaa gtcgatctac ggtgaaagtc gttttgtaga tattactgac ttgaatgaat
     2941 ttcccaagca aatgattcaa ctgattgccc gctatctatg ataaaataaa gctcaacgaa
     3001 cgcacttagc agattcgttg agcttttcgt ttctagttac cggtaacaag gatacttcta
     3061 aaatagaatc ggttataatc aagagggacc gttaaaggag tgaaacgata tgatacaaga
     3121 acaaaaatgg tggaaagaag cagtaggcta tcaaatctat cctgctagtt tcaaagatag
     3181 caatggcgat ggtcaaggag acatcaatgg gatccgtgaa aaacttaatt atttgaaaga
     3241 tcttggtatc ggctttatct ggatcaatcc gatctatcaa tctccatttg tcgataatgg
     3301 ttatgacgta agtgactatc aagatataga agagagattc ggaacgatgg aagattttga
     3361 tttattgcta aaagaagcac atgaattagg gatcaaagtg attatggatc ttgtcatcaa
     3421 ccatagttcg gatcagcatc aatggtttga agaatcgaaa aaatctaaag aaagcccgta
     3481 tcgcgattat tacatttggg tcgacggggt agatggcaaa gaaccgaata attggacatc
     3541 gattttcggc ggttctgctt gggagtattc tcatgaaact ggacagtatt atctgcatgt
     3601 ttttgcaaaa gaacaaccag acttaaattg ggaaagcgag aaattgaaag aagaactatt
     3661 caatatgatc cgatggtggt tggataaagg gatagatgga tttcgtttag atgcgatttc
     3721 tcatgtaaaa aaagatgaat attcagtaaa agccacagag aaccctttct caccctttca
     3781 aaatgtctca ggaatcgaag aacatttgac cgatttgaaa catgtttttg aagagtacga
     3841 tatcatgact gtcggagaag caagtggtgt cactgcagaa gaaggaccac aatgggtagg
     3901 gaaagacggt tatttcgata tgatattcga atttgatcat atccatatct ggcagcaaga
     3961 aaaagaaggg caattagatg tccttaaatt gaaacatgca ttaagcgctt ggcaaacttc
     4021 attggatggt atcggatgga acgctttata tatggaaaac catgatgttc ctcgtgccgt
     4081 ttcagtgttt ggagatactc gtcccgattt ttgggctatg tctgcaaaag caatcgctat
     4141 gatgtatttc tttttgcaag gtacgccatt tatttatcaa ggacaggaaa tcggaatgac
     4201 gaatatgccc tttgaatcga tcgatcaagt tgatgcagta gattcgaaaa gactttataa
     4261 aaggctgctt gcggaaggta agacaagaga agaagcattg gatatcattc gtgaaacaac
     4321 gagagacaac agcaggacac ccatgcaatg gacttcggaa caatatgctg gtttttctac
     4381 acatgaacct tggctgatca ctaatccaaa taccaaaacg atcaatgtag aacaacaaga
     4441 atatgaacca gagtcggtcc tgcaattcta taagaacatg atccgcatta gacaaaccaa
     4501 taaaggtctg atatacggtt cttataaaga atatcttcat gaacatccac aactttatgt
     4561 ctatgaaaga tatttagaag atgaacaata tcttattatg gtaaacttaa cagaatcact
     4621 tgctgactat gaacttccaa aagaagcaga tcagtcctgg acattactct tgtcaaattc
     4681 ctctagcgga gaatttgaag ctaaaggcat actagcacct tatgaagctc ggttatataa
     4741 aacaaacaag taaaacttcc tcgttaaagt tcattagttg gactttgcga ggaagttttt
     4801 cagattgctc tgtcaattac tcatttcaat aattcgctct aggaccaccg atatatttag
     4861 gacgagagcg aagggctgtc acgtgagctt gtccaagggt tttcaaacga ggtctgacag
     4921 gtacttggac atgcttgata tgcataccga ttgatgtgtc accgatatcg ataccggctt
     4981 gtccaactac gtgttccact tcgacaggat cattgaactg gtcaaaagca gcaacagaac
     5041 aagcacctcc agcatgtagt gcaggaacaa cagagacaat ttcgaaattt tttgcttctg
     5101 ccactgttcg ttctactact aatgctcggt tcaaatgttc acaaccttgg acagctagcg
     5161 caatttcttt aggatccaac aactctttta acgtacggat gatccattga ccaacttccg
     5221 cactggaatt ttttccaata tgtcctccaa cgacttcgct tgtgctgcat cctaaaacaa
     5281 aaagatcacc ttttttcaag tgtgcctcag ccaatatgtc attaactatt tcagtcaatt
     5341 ggtcttttaa ttctttttct gacaacttca ttttctaaaa ccttcttttt caattaatat
     5401 tgcttccgtg cttgaatacg ttgaagactt gcagttatag gaaaggcaag tcccattcca
     5461 atcagatttt ggaagatgtt tcctggtata gaagccactc cagccggcca atcatataaa
     5521 atccaaccgg cgataccata gccgatgatc ataactaagc ttcctaataa aagccctaat
     5581 ccccacatac gagtagaaga tttgctgaaa gaaccagcaa catatccttg taacccgtgg
     5641 atcactaatg aaaaaactgc ccactgcgga tatcctgaaa tcaaatcgat caatagacca
     5701 ctggaagcac caacaactaa tccacccgtt ggaccataaa gaaaagcagc ggtatagatc
     5761 ccggcctcgc ataatgtcac aaatccattc gtagccggta cagggatcaa aattgttaat
     5821 gaaagggcaa ctgtcaacgc aatcagcata gcatagatcg ttaattgctt agttttcatt
     5881 tctcattcct cctttttgaa taccgccaga ccagacactt ccggtatgat cgttcatcag
     5941 cacgccatta gaaatacaat gggcaacaaa ttttttactt tctgctactg ctttggtaaa
     6001 atctgcccct aacacaagat tcgctgttat tgcagaagaa aaggcacaac cggccccgtt
     6061 tactgttgta gtgtcgattt ttttatgaga aaatacctca taatgatcaa ttgaatagag
     6121 aagatccaca gcttcatctc cagccatcct tgtgccgcct ttgatcacaa tgttttttgt
     6181 tcctgaagca gtgagttctt tagcgagatg gatcatttct gttaacgaag agggaagagc
     6241 ttgaccagaa agtaaagcag cttctgccaa attaggcgta atcatcgtaa caagtggaaa
     6301 aagttcttcg atgagctgcg aaatatacaa ttgattgatc acagcttcag tttctttgaa
     6361 tgccaaaaca gggtctaaaa tgattgggat attttgtttt gtttttatga agtctcgaac
     6421 aaccatgatt gcttcacttg aattcaataa cccaattttg ataccatcta atgaagtcat
     6481 tttatcaatc gaaactaact gtcgtttaag caattccaaa gatatagatt ccagcataaa
     6541 tcgctcatta atatctagtg cccctataga agtcaaggcg cttagtccga atagaccaaa
     6601 attctcaaaa gtttttaaat ctgtttggat tcctccacca gcaaatggat cagaaccgcc
     6661 aattgtcaag attcttttca taacattttc ctccatgaat aagagtataa cgaaattttc
     6721 aaaaaagaaa aacagccaat tggcatattt ttatctcatc caatttaaaa aaacaaaaag
     6781 aaaagaaaga ccaaaatgga tctttctttc cttaaatagt taggactttt gtgattttaa
     6841 ttctttctcg atttgaagcc gttgcacttt cattgtagct gttcgcggga ttgcatcaaa
     6901 atcacggatg atagggacgt tcaagcgagg aagttcatgg acttgctccc accatcgatt
     6961 ccaatccatt tctttatcag gagctaaagc aataatcggt tgtggagaat gatttttatc
     7021 ccttacaatc actacttctg ccaaaaaatc taacgaatct aataaaaagt cttcaatagc
     7081 taatgtacta ttgatcgttt cgatcaaatc cacttgccga tctttcaaaa ataggtggcc
     7141 atgcttatcc ataaaaccgt aatctccgct atcccaccaa tcgccataaa cattttcctg
     7201 gaaccgtgca tcttccttgt aataagtcaa agcccgtcct ttagacagta attgtatatg
     7261 accatctgtg ttttcaggta ggacattccc aactgaatct gtaatccgag cgcttgtcat
     7321 gtcttccagt ccaacgccca tatctcttgc atcacttgtt ttcaaactct ctagtgtatg
     7381 agccttaaga atcattggtc cgcattcact ttggccatat acttgaagaa ataccgcatc
     7441 ctgtgccatc gacgttcgca aaaatgtctt cattgtctgg ttgtttatag catcaaaagt
     7501 ggagtgatag tatcggatac ccgaaaaagc ttctggatgc tccttagcag taaaacgcca
     7561 ctgaacaaag ttattcggat gggtctctag tgcaattggc cggtgagctt ccaacatatg
     7621 aacaactttt gaactttgtg cgtttgctaa aggcatcata ggaaatccca tggacatcaa
     7681 agaagagata ccaatattga atctggaatg cactggagag atgtgaaaag cgattaattt
     7741 cttttccgaa atttttgtga aaactgtttt ttgccatttc gtccgccatc ccatagaatg
     7801 agcagaatga caaataagtt tagggatacc agttgtacca gaagtatggg tcatataaga
     7861 aatttcattt ttgtctaaaa atgattgaga aacagacaaa gcaggttgtt ccattaaata
     7921 aagaatagga atctgttttg attttgttcc atttgaaatt tctctcactc gcttttttgt
     7981 ctcatcatca aataaaatga aagggtcttc taaacgatcg ataaatactt ccattgttgt
     8041 tgttggtaga tgataagaag tcatgacagg cactgcacca aggtaagaca cagaaacagc
     8101 tagaaggtat gtgtcaaaag cggaactctt ataaataata actttgtctc cggatttcac
     8161 tccaagatga gccaattgat aagcccgttt caacacactt tcgcagcatt ttttgtaagt
     8221 tgtttcaagt cctaattctg ggaaagctga aagtggctca tcaaagataa tcggggtctc
     8281 ggaaaagtta gctgcagctt cttgaaaatt cgtaaataag ttcaaagggg aatacgttat
     8341 ggtctccatc attcacactc cattatcttt tgttaagtaa tatacaaatt caagataatc
     8401 gtaacataat cctttatata ataacatagt ttattgtgaa aaaaagatca tacatttgtt
     8461 ttatatggaa gaaacccgaa cgaatgccgc taattcgtga taagaaaaat caaccatctt
     8521 ttcccagata tcttgaaaag aatgggatga gaggtatcga ttaccacata aataatttac
     8581 cgcgtcagca tcatctaaat atagttctgc ataataacga tatgtttttc tgcatagttc
     8641 tggcgatacg tttttatata ctgtaatttt gctcaaaatt tggcgaatag tccgaggaga
     8701 aatttgctga tcatgtgagg aacgaaaaac aaaagaattt tcgtccgtat tttgtgctgt
     8761 taacttaaaa tagttctcca gaaatggcat cgcttctttt ggtattggta ctttattcag
     8821 tctgttttct tttctattca gattaagaat ctgcttggtc aaagatacat ctttacgttt
     8881 aagttctact acttcatgga tctttaatcc gcaatacaga agaatggcag taattgcaag
     8941 gttcctcggt ctattctttt cttcaaagct agcttctctg cttgtttgaa agccttcaat
     9001 cgggataaac aaatgctcaa aatctttgaa aagttctgca tcgatatatc gcggagctgc
     9061 tttggttgtt tccggtaaac ttgtacccaa aagagaaatc tcttcttcaa tcagtccttt
     9121 ttgatacaaa aatcgtaaaa aatgatttaa tgaatattgc ttccttgaaa tcccgtaata
     9181 gctattttca aacaattgct ccgtttcttg gttcgctgta gtttggaacg aacgagtatg
     9241 tcgatagatt ttagctaaga aattctgata atcttgtttt tctaaatgga taatcagctg
     9301 atcatccact tcttcagaaa atacttttct caaataatca gtaaacagat gaaaatcttt
     9361 tgcgtattca tagcgactgc ctaatgaaag ggaagaacga ttttcttcta aaaactgttg
     9421 aaagacggaa gggagcaagt gtgtacgttg ctttaacttt tcttgcagtt gttttctagt
     9481 tttgctgtcc attacaaatt ccctccaata gtgttcattt tctatatttt accgcatttc
     9541 atcaaatgaa gatagacagt ttgtcttgat cttaggaagc aaatttttaa ctaagtacta
     9601 gctaaaagat atgaagtaag aagatacatt tttagttatt ttattagtgc agtcattctt
     9661 ttgatctttt aattgattta tcctcttttt agctatgtgg ttacttcatg agcaatgaaa
     9721 tggatttggt tcgttctaat ggcaggaggc tctttctttt atttccgaaa aattagaaaa
     9781 cattttttca gaatcgattg gctctccatt tattcaataa tggtttgtct aacgatattg
     9841 ttgggataat tcttaaagaa gtattagaaa tagttggtac ttcattactt tacatgaaga
     9901 tttattctat ggtaggagca attatattga gaattaatcc ttttttatat aagttgtttg
     9961 cttattgatg gattg
//
LOCUS       DAYYCY010000096         9918 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000096 DAYYCY010000000
VERSION     DAYYCY010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9918
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.096"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(79..591)
                     /locus_tag="ACHAZB_001895"
     CDS             complement(79..591)
                     /locus_tag="ACHAZB_001895"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFM8614535.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(605..2902)
                     /gene="recJ"
                     /locus_tag="ACHAZB_001896"
     CDS             complement(605..2902)
                     /gene="recJ"
                     /locus_tag="ACHAZB_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFM8614536.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(3044..3487)
                     /locus_tag="ACHAZB_001897"
     CDS             complement(3044..3487)
                     /locus_tag="ACHAZB_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HFM8614537.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(3488..4276)
                     /locus_tag="ACHAZB_001898"
     CDS             complement(3488..4276)
                     /locus_tag="ACHAZB_001898"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFM8614538.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(4273..5214)
                     /gene="rnz"
                     /locus_tag="ACHAZB_001899"
     CDS             complement(4273..5214)
                     /gene="rnz"
                     /locus_tag="ACHAZB_001899"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HFM8614539.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(5391..5978)
                     /locus_tag="ACHAZB_001900"
     CDS             complement(5391..5978)
                     /locus_tag="ACHAZB_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HFM8614540.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(6199..7512)
                     /gene="obgE"
                     /locus_tag="ACHAZB_001901"
     CDS             complement(6199..7512)
                     /gene="obgE"
                     /locus_tag="ACHAZB_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HFM8614541.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            7800..8810
                     /gene="gap"
                     /locus_tag="ACHAZB_001902"
     CDS             7800..8810
                     /gene="gap"
                     /locus_tag="ACHAZB_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFM8614542.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(8895..9830)
                     /locus_tag="ACHAZB_001903"
     CDS             complement(8895..9830)
                     /locus_tag="ACHAZB_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8614543.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         2728 a         2270 c         1715 g         3205 t
ORIGIN      
        1 atagagaatc tgacttttga gggtcgcttt aaaatcgctt ctttaagaat aacatcaaga
       61 atagcttctt ttttctgctt aatattccat taatgtaatg atgtcatatc ctttgatttt
      121 gtcgcgtcca tggagatcca tcaattcgat caaaaatgcg cagcctacga ctacaccgcc
      181 taattgttcg atcagttcga tcgtcgcttt gattgttcct ccagtagcta gcaaatcgtc
      241 acaaatcagc acacgttgac ctggtttaat ggcatcctta tgcaatgtca atgtatctgt
      301 tccatattct aatccataag tcacttcgat ggtttctcga ggtaacttcc cttttttgcg
      361 gactggggca aaaccgactc ctaactcata agcaacagga catcctacga tgaatccacg
      421 ggcttctggc ccaactacca tatcaattcg tttttctttt gcataattta cgatttgttt
      481 agtagcttcc cgataagcgt caccatctgc catcagtggg gaaatatctc gaaacacaat
      541 tccttttgac ggatagtcgg gaatactggc aatataatct tttaaatcca ctctttgttt
      601 cctcctaggt tgttaaccat ttctttatgg taggaatatc gctcaaaagc aaaaattcct
      661 caactttgat tttattgagc cgtcgctggt ataatctgct ctcagtcaaa ggatgacttt
      721 ctgggctagc gactttttgt aaaacaccgt tctctattgt aacaaatttc aattcaaaaa
      781 acacctgtat cataaaaatt aataattttt cagggatacg caaatattga gctattgctt
      841 ttattttatg acgtatatca attcgttctt gagaatggat caatttaaac aattttgcgt
      901 actgttctct tgtgcctacc ccattcaaat aggcctcatc aggtgaaatc cccatcagat
      961 aaacacgatt gaatgaataa gaccgaaaaa cttctttcag atccgctaaa tcatctggac
     1021 aatccataac aactagagag tttggttttc ttttttcaat tattgccttt acagaatcta
     1081 gtccttcgaa aacaatcgca gcttcctgga taatctcagg cacgaatttc aacgtcgaag
     1141 aatcaaatgc taaataaagg ctaccttccg acgttcggct ctgttcacgg aaacgttttg
     1201 cccgccaatc aaatacttgc agaccaccca cagaaaaatc agataccatc agctgcggtt
     1261 tcttacgccc gttccattca ttgatcgaaa gctttcctac cacatctacc tgattattca
     1321 gcagttcttc ttcttgagta ccaaaaccaa aagcgaccgc atcaagctga ctcgatgcat
     1381 cggaaaggac aaatttcaga tgctgttggt ttgctccgat tcttttgata ttttctgctt
     1441 ggacttgtcg aaataaaaac tgaggcaaag gattatccgt accaaatggc gccaatagtt
     1501 tcagctcatc aattcgctct actgttactt catttggcag caatacttca tcaattctca
     1561 actcaggccc tctcatcaag tcaatttgat gatcgactat atattggttc atcttctcct
     1621 gtagtatagt gacattttca cttggcatcg ttaaaccgac tgctgcatga tgtcctccga
     1681 agtaagtaaa taaatctcgc atctggtcaa gcatttcaaa aagattcaag gcttcaatac
     1741 tacgtccaga tcctttagct gatccatctt cttttaaagt aaggacaagc gttggtttac
     1801 ccgtttcatt cattattttc ccagcaacaa tgcccaaaac gccttcatgc catcctcggt
     1861 ttaccaatag atggatatga ttctccttat tgaccatcgc taatgcttct tgtgttatct
     1921 gctctacgat tgcttttctt tcttcattga tctcattcaa tttttttgct tgaacggatg
     1981 cttcctcttc atcaaacgtc gcaagtaacg agacggcagg acgtggatcg cccatccgac
     2041 cgattgcatt tagtctggga gcaatcgaaa aaccgatcgt tgtttcgtct gcgtccctca
     2101 tttttacgcc gctttcttca aatagcgcct gaagtcctat ccgttctgaa tgatgaatgg
     2161 catcgattcc taatgaaaca aggattcgat tctcatcagt caatgaaact aaatcggcga
     2221 tcgttcctat cgctactaaa tccaaaaatt ctgcaggcgg ttcttccaat aatgcagaag
     2281 ctactttgaa tgctactccg actcctgcca aatctggaaa tggatagtgc cccgctggat
     2341 gacgagggtg aacaatcgca taagcgtctg gtaaattctc aggtagttcg tgatgatctg
     2401 taatgatcac gtctacacca gcttgctgag cgtatgctac cgcatcattt cctgctaccc
     2461 cgttatcaac agtgatgatc aactggattc cttcttcgat cttttcttgg tataccgatt
     2521 ggtttggtcc atacccatgc tcaaatcgat ttggcaggaa tacttctact tctgcaccaa
     2581 gtaattccag tgtttctttc attaccgttg cactggtgat cccatctgcg tcataatcac
     2641 cgtaaacgag gattttttct ccactgatta cagcttcttg gattcggctg attgctttat
     2701 ccatatcgtg tatcagatac ggatcatgta attcttgttc ctttggatgt aaaaatcggt
     2761 tgatatcctt ttcctcatgg taacctcttt gccacagtaa cttaccgaca aatggagata
     2821 atttcagttc acggatcatt tggagaaagg attcagaagg ctctgtctcg cttaacttcc
     2881 aatcaaaatt tgcttgtctc actttctcac tcctaactta gttattatag caaaggaaat
     2941 cagcaaaaaa aagcagaaag aaagaataaa aacagatcag atccgctaga cagatgttca
     3001 gcaggacctg atcttttgta cgaccattta tgaaatatcc ctattagtca aaataattga
     3061 agctttgttt gggcgattga gttttttgtt cttccggcgc tttcaattga ctgatttgtg
     3121 cggttttcgc ttgtaactcc gcctgataag cagcttccaa ctccgcacgt tcgtttctaa
     3181 actcgcgttg ctgttcttct tttgcttctt caattttctt ttgaagttct gtttgatatt
     3241 ctgacatttc ctgagtcagc tgtttgattt gctttttttg ctgccatagt gttgccgaag
     3301 cagtcaaaaa tacaatcagt gcaccaatca gcgcagaacc gatgatcact aagatcaatg
     3361 gcgcagaaaa ggaagtgaag ccaaaactta caggaacatt tttattgttc aatacagcaa
     3421 aaagaacaat aatcaaaact aaaacaaatc caagaatcac attgctttgc tttttcaatt
     3481 ccttcaccta ctttttgtta aaaattccgc tggctaaata atcaccaatc gttggaaaca
     3541 gcgtataaaa ttttgatgca acttctaaaa taaacggttg attgatttcc cgttttggat
     3601 gtctcatatt gcggacgatc atttttgcca acttggcagg ttctaaaacg atttgtccga
     3661 ctttttccaa atagcttccg cttggatcag ccttatcgaa gaaatttgtc tcgattggac
     3721 ctggattcac tgtcgtaaca gctacgccaa gtggtttcaa ttctaatcgc aaagcgttgg
     3781 agaagccaag gactgcaaat ttcgtggcag aataaaccgt tgattttgca gttgccattt
     3841 ttccagccat agaggcgata ttaatgatat gaccttgtct tctttctgcc atatcgatcg
     3901 caaatttttg tgtcaaaacc atcattccta agatgttgac ttcaaacata tcgtatgctt
     3961 ttccaaggtc gaatgtaagg aagtcttcga aaatcccaaa acccgcattg tttaccagaa
     4021 tatcgacgcg cccgacttct tccatcacct tttcatataa ttcgtccacg ctttcaggat
     4081 tgctgacgtc taccttgaaa gcataagcct ctttgccgct caagcgtctg cactcgtctc
     4141 tgacaccttc tacaaatgcc attcttcgtg cgcaacttat aacgattgct ccttgctttg
     4201 ctgcttcata acagatttgt gcgcccagac cgcctgaact acctgttact aaaacaactt
     4261 tatcttctaa attcatgcct cttcctcctc gcgaaatgga atctcaatgc tatcaaaatc
     4321 tttcataatc gtcgtgtttt caaacacttc ttgtgcttct ttttcaagct ctaaagctgc
     4381 ttttcctaaa tatctcgcac tgatatgagt aagtagcagc cgttttgcct gcgcttcttt
     4441 tgcaacttca gcagcttgat gagtcgtgga atggaaatag ttgtgcgcca ttcttgcttc
     4501 atctttatta aatgtgcttt catgtaccag aatatcgcta ttttccgcaa gcacaacgga
     4561 attatgtgtt tttctcgtat cgcctaaaat agtcacgatt cgtccttttt tatctggacc
     4621 tacgaaatct tttccattga tcgtccgccc atcttctagt tggatcgttt caccttgctt
     4681 cagtttgccg tacagtggtc cagctggaat acctaattct tttagacgat ccacctgcaa
     4741 ttctccttta tgatcatgtt caaccacacg ataaccaaaa ctgtcgatcc catgatttag
     4801 tttttttgta taaacagaaa attgctgatc agtaaaaatc ggggctgttt catttagctc
     4861 aatgaatttc agcggataac ttaaacgcgt ttgcgaaata cctaaagaga cttttatgta
     4921 ttcctctatc ccttttggcc cgtaaatctc taatggcgta tccccacctt gaaatgaccg
     4981 gctgctgatg agcccgggaa gtccaaaaat atgatcgccg tgtaaatgtg taatgaaaat
     5041 tttaccgatt tttctcggac gaatagtcgt tcgcaaaatc tgcatttgcg tgccttcgcc
     5101 acagtcgaac aaccaaactt cattgcgctc gtctaaaagt ttcaatgcta tacttgtcac
     5161 atttctatgt tttgcaggaa cacctgcacc tgttcctaaa aattgtaatt ccataatcta
     5221 cctgactttc tttaagttcc tctttaattt tagggtaaaa agaagggata atcaaccgta
     5281 agtcgagaat gggttcaaat taaaaataac gttctaaagc gatagcgttt tctttgaaaa
     5341 ttactataaa aacagcagcg aacccgtttg agatcctctg ctgtttttta ttaatttgaa
     5401 gatccagaac tttcattgtc gttggaagtt tgcggatgtt tgtccttcca agtcttttgt
     5461 gcagcttctt tcgcatgatt ttcagcttct ttcgcatcat gcacatcttt ttcgacatct
     5521 tcataatcca agcgagtaat ttctccgcct aattccatca ttaccaattg attcagtggg
     5581 cgattgtcct cttcatcagc aatcaagatc agtcctgtag ctccttcacc gattttattt
     5641 cctacaaagt cgaacacggt ctgggcatct tttatttctt tggcatcttg tgtcgcaccg
     5701 atcattcctc cggcaaacca tcctaataaa atccctagcg gaccacctaa tacaccaacc
     5761 aacattccga ttaatccacc cgtagaagat ttgttattac cggtaaagtc gataaaatct
     5821 tctattttga attgatgtgt cccattagga tcatggctga caactgccat ctgttctcct
     5881 ttaatcgaac gaacagcatg taactttttg atttctgaaa atgcctgata tgcctggctt
     5941 tctacttgaa aatttaaaat gattactcgt ttgctcatta aaaacgcccc tttcatacct
     6001 tcttgttatt tattttacct ttaattgaaa aataagaaaa gaaaagcact ctttttttcc
     6061 aataaaatag ggctgtgaca aaagtcgtat cacagctcct ttttccgaat aaacggcgtt
     6121 cttgaagctg atcacttctg tcacaacctc ccttaaatct gaatctatat tttttggtat
     6181 ttgtttttat ttgcttgcct attcgacaaa ttcaaattca aactcaccga tacgtacaag
     6241 gtcgccatct ttcgcaccgc gtgcccgaag agcttcatcc acacccatac cacgtaattg
     6301 acgagcaaaa cgcatcactg tttcatcatg atcgaaattc gtcatttgga ataatttttc
     6361 gatcttctca ccagataaga tccacgttgc atctgaatca cgatcgattt ggaactcagg
     6421 accttctgga ttgaatccat agtgtacagt ttcttcttcc agttcttctt cgtacaatgg
     6481 gaattctggc gttacttcta acaaatctgc tgtagcattc aataacgcgt ccaatccttg
     6541 tcgagtcact ccagaaattg gaaatactgg gatgtcatca gcaaattcat cttctttttc
     6601 cttgttcagc tgttctttga atttcactag attttccggt gcatccggca tatccatttt
     6661 gtttgccacg atgatttgcg gacgctctag caagcgtaag ttataagtag ataattcttt
     6721 gttgatggca agatagtctt cataaggatc tcgtccttcc atcccactca tatcgatcac
     6781 atgtaaaatt actcgtgttc tttcgatatg acgaaggaat tgtgtaccca gcccaacacc
     6841 ttgcgaagct ccttcaatca agcctggcaa gtctgctgcg gcaaaacttc tgccatcact
     6901 tgtagtaacc attcctagat taggtacgag cgtcgtgaaa tgataagcgc cgatttttgg
     6961 acgagcagat gaaataacgg atagtaaagt cgatttcccg acagatggaa aacctacaag
     7021 tccaacgtct gccaatactt tcaattctag ttcgattttt cgctcttgtc ctggttcacc
     7081 gttttcagca atttccggtg caggattccg aggagaggca aaacggatat tccctcgtcc
     7141 accgcgtcca ccttttgcca caactagtgt ttgaccattt tcaattaaat ctcctaaaag
     7201 agcaccagtc tccgcatctc ggacagtggt tccttgtggt actttaacat aggtatcttc
     7261 tgaaccacga ccgtgcatcc ctttactcat cccattttca cctggctgcg ctttgaaatg
     7321 gcgattgaac cgaaaatcca tcaatgtacg taacccttca tcgacgatca agatgacatc
     7381 gccgccacga ccaccgtctc cgccagccgg tccgccgtca ggaacgtatt tttcgcgacg
     7441 aaatgcaacc attccgtctc cgcccttgcc agctttcaca tcgatcgtta cttgatctaa
     7501 aaacatggac ataattaagt cctcctatta ttcatttatt tatttgactg cctgaatttc
     7561 cacaataaac aaaagtctta cttattttat cggtgaaatc tgattttgtc tatcatttca
     7621 gcaaaaagat tgtttacctt cttctttttt tatcatttgg taaggtttcc aacctttttt
     7681 ctgctaccaa gtataatttt ttgacaaaat attctttgcg tagcagaata acagtgtatt
     7741 aattgtatta attgaggatg ccgtttcaca gcacccctaa taaaggagga acttttgata
     7801 tgacagtaaa agtaggtatt aacggatttg ggcgtattgg ccgtctggct ttccgccgca
     7861 tcaaagaagt ctcagatgat attgaagtag tagcaatcaa cgacttaaca agtccaacta
     7921 tgttagcaca attattgcaa tttgactcta ctcatggtac ttatcctggc acagttactg
     7981 caacagaaga ctcaatcgta gtggatggcg aagcaactcg tgtttatgca gaacccgacg
     8041 caagcaaaat cccttgggca aaagaaaacg gtgtagatat cgtactagaa tgtactggtt
     8101 tttatacatc tgaagaaaaa gcaaaagcgc atttagatgc tggtgtcaaa cgagtagtta
     8161 tttctgcccc agctggtgct atgaaaacca tcgtctataa cgtcaatgat gatacattag
     8221 atgaaaacga caaaatcatc tcagcaggtt catgtacaac aaactgttta gcacctatgg
     8281 cttatttctt aaataatgaa tttgggatcg aagttggtac aatgacaaca gttcatgcct
     8341 acacttccac acaaatgctc ttagatggac ctgtaaaagg cggtaatttg cgtgctgctc
     8401 gttcagctgc tgacaatact atcccacatt ctactggtgc tgcgaaagca atcggcttag
     8461 ttatccctga gctacaagga aaattacaag gacacgcaca acgtgttcca gtcgttgatg
     8521 gttcattaac tgaattggtt tcagtattga aaacaaaagt aactgctgat caagtaaacg
     8581 aagcaatcaa aaaacacaca gttgacaatc cttcattcgg gtatgatgac cgtcaaatcg
     8641 tttctagcga tgtcattgga acaactcaag gttcgatctt tgacccaacg caaacagaag
     8701 taacaactgc tggtgatttc caacttgtta aaactgttgc atggtatgac aatgagtatg
     8761 gatttacttg ccaaatgatt cgcttactag aaaaattcgc taacttgtaa tttgatagat
     8821 caatcgataa aagaagcctt tgactggatc cagtcaaagg cttctttctt ttcatcccct
     8881 acttttaaac tcgttcaatg aacatattgg cttaaaaatt gttggactac ttgctgatat
     8941 ttttcaggat ctgtcttgta tgcttccgca tgctcggctc cttttacaac atacttttct
     9001 tttggtccct tcgttgcacg atagacctta tctaacattt caaatggtac aaatgtatct
     9061 tcgtctccat gtataaagag cattgggcgc tggtttttct tcaattgttc cactgcattt
     9121 gcttcaccga aaaagtagcc agcacggact tttgtcacta gactcgttac ttgcataagc
     9181 ggaaatgctg gaagactaaa catatcgtct agctgataag caagctcatc atgaacggaa
     9241 gaataaccgc aatcttcaat gattgctttc acattatctg gtagtttttc tccgctagtc
     9301 atcataactg ttgctgctcc catgctgaca ccgtatagaa cgatttcttg cgattttcca
     9361 ttgttttcta gcactttgtt tatccactgt acatagtctt tacgctctgg ccatccaaat
     9421 ccaatgtaat ccccttcgct ttttccatgt cctcttgcat caggaacaag gacattgtaa
     9481 cccatatcat gaaacatctt tgcgtaattg gtcatcgttt cagcgtttcc catatagcca
     9541 tgagccacaa gagctgtttt ttcacttttc gtttcagcag gaagataaat cgctgataat
     9601 ttcagaccgt cttttgaagt gatggtccaa tcgcttcggt tttcttttga actaaaccat
     9661 ttatgtgcat ccgtttcttt ggatgtctgt gttgtaccag gtgtatcttc gtctaaaaag
     9721 tctttctcag aaggcacaac agcataatta taaaaataat tccctgcaaa cactaacgca
     9781 acaatcagag ctaaagcgac gcctaaaagg attttcatcc aaagtttcat aaatcatcca
     9841 tctcatttct cttttttcat ataaccttga attagtataa tcaaagaatc tctactatgc
     9901 aatgttcatt tttataac
//
LOCUS       DAYYCY010000097         9615 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000097 DAYYCY010000000
VERSION     DAYYCY010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9615
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.097"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            89..283
                     /locus_tag="ACHAZB_001904"
     CDS             89..283
                     /locus_tag="ACHAZB_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8614544.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            552..851
                     /locus_tag="ACHAZB_001905"
     CDS             552..851
                     /locus_tag="ACHAZB_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8614545.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(948..1382)
                     /locus_tag="ACHAZB_001906"
     CDS             complement(948..1382)
                     /locus_tag="ACHAZB_001906"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8614546.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1614..2324)
                     /locus_tag="ACHAZB_001907"
     CDS             complement(1614..2324)
                     /locus_tag="ACHAZB_001907"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFM8614547.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2314..3174)
                     /locus_tag="ACHAZB_001908"
     CDS             complement(2314..3174)
                     /locus_tag="ACHAZB_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFM8614548.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3179..3817)
                     /locus_tag="ACHAZB_001909"
     CDS             complement(3179..3817)
                     /locus_tag="ACHAZB_001909"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFM8614549.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3832..4131)
                     /locus_tag="ACHAZB_001910"
     CDS             complement(3832..4131)
                     /locus_tag="ACHAZB_001910"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8614550.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4174..5658)
                     /locus_tag="ACHAZB_001911"
     CDS             complement(4174..5658)
                     /locus_tag="ACHAZB_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFM8614551.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5678..6139)
                     /locus_tag="ACHAZB_001912"
     CDS             complement(5678..6139)
                     /locus_tag="ACHAZB_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8614552.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6157..7224)
                     /gene="ulaG"
                     /locus_tag="ACHAZB_001913"
     CDS             complement(6157..7224)
                     /gene="ulaG"
                     /locus_tag="ACHAZB_001913"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFM8614553.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7442..8212
                     /locus_tag="ACHAZB_001914"
     CDS             7442..8212
                     /locus_tag="ACHAZB_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM8614554.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8485..9312)
                     /locus_tag="ACHAZB_001915"
     CDS             complement(8485..9312)
                     /locus_tag="ACHAZB_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8614555.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
BASE COUNT         3001 a         1959 c         1503 g         3152 t
ORIGIN      
        1 tatttatcat aaagatatag atacataatc attttcactt acatatattt gaatattaac
       61 taaaaacagc aagaaagaag aggatcaaat gtattatgta gaagtaaaaa caaaaggcgt
      121 caaaaacaaa caatatgtga aaggtatcag taacgaatat cctttacttg gttcatggaa
      181 agaagctgct cctttttcca aaccatgtgc cataaaaata aagaacgagc tagaaaaaga
      241 actgacttgt ggtaaagctg ttgtagatat catcgaaaag taaatccaat gtatagaaag
      301 gaagatcaca catgaaaagc gacgtacaaa ttgctaagat tatagaatta gaagaagaag
      361 acgcagtgtt gacaattgaa aaaaaataaa tgtttgtata ccatatgatt atttcaatat
      421 gtatcctgga attggagatc tcgtagagct atatcaagat gaatcaaata tacttgtcat
      481 tcatcatgaa atatagagac ttataaaatt gtttattttt ataatgtatc agtatagaaa
      541 ggaaataaaa catggattat actgttgaat ttgacagtga actacttaag caatttccac
      601 aagaagaaca tttcatgtat aaagaagcaa tttcccacaa agtgacagag tatttggagc
      661 ataatttttt tcgtataaag ccagtgcgta ctcgttcggc aaaaggaata tacgagatga
      721 aagtcaaaat taataaaaaa aattatcgca tagcatttgc tttaaaaaaa aatcatgtgt
      781 tcatttttta tattagttcg aatttgcaaa aaattgtatt tgataaagag gtgagtaaaa
      841 aaataaaata gctgagcatt ggaggataga ttgagtgata ctaagtctat cctcttgttt
      901 ttctaaagaa ctaagctaga attttttctt ttgtacgtta ttatatgtta tttgtcatta
      961 ccaaaaagca ccttttttct tctagtatct atgacataag aaataccacc catttccttt
     1021 acttgaacaa atcctactga ttcataaaat ttttttgttt tctcagtatc atctgtcaat
     1081 aattcgattt ggactggtcc ataactagtg atcttctcca aagcttcatt caaaagcact
     1141 ttgcctattc cttggcgctg atacctcgga tgaatgagga tatcttgaat aaatgcgatc
     1201 gtcgcaaaat cagtgatcca acgaacgatt ccgataatct tcccttcccc attacgcttt
     1261 atcagagatt cagattgttc aaatgctcta gctaaccttg cagtgtcttt tgtgtaattc
     1321 gtccaaccaa cgctcttata taattcagaa atctcatcga tcataaggga atcaatattc
     1381 atttttttct tcctttctta tacaattctc cctctaataa tacagttctt tgactattta
     1441 atctatcaca tataactctc ctgtaaagat tctctcgatt ccttaaaatt cctctttcct
     1501 tttagaatgc atactttttg ataaataaaa aggttgtgat aaattttctt cacaacctct
     1561 gtttctctct atcaagtgtt atcttttaat tttctaccct taccctagca tctctattgc
     1621 ccgtagtatg catactttcc atgtttgcga aaataatgtt tatctagtaa atattgcggg
     1681 atttcacata tattggggtt gatcagctcg ttttctttgg ccatcagaca aatttcatct
     1741 aagatcaaac tgttttctac tgctttcata ggtgaatccc cccatgtgaa cggtccatgt
     1801 ccatagacaa gtactccagg gacttcgttt ggatctaatt ttctttcctt aaacgtctcg
     1861 acaatcacat tccctgtatg aacttcgtaa gcttctctta cttcttcttt cgttaattgg
     1921 cgtgtacaag gaaccttacc ataaaaagca tcagcatgag tcgtgccata agctggcaaa
     1981 tcgcgtcctg cttgcgccca catgacggag tgcgtggaat gagtatgagt aatagcagtt
     2041 atatcttcaa aagtttgata caaaacaaca tgtgtgggga gatctgaaga tggttttaag
     2101 ctatcttctt ctattatatt tcccgacaaa tctgttacca ccatttgatc tgcttgcatg
     2161 cattcatatc tgacaccgct tggcttaatg acgatgactc ctagctcacg gtccacttca
     2221 ctcacattgc cccaagtcaa tttgactaat ccgtatcgcg gcaatgccag atttgcttca
     2281 aggacacgtt gcttcattgc tgcaattttt tcttcacaag acatcatagc cagcctcctt
     2341 caatttagaa cttaagtagt cttttgcctg ctggatttct cttcgaaagt cttggctttt
     2401 ttcactccac atttcaatca gaaatgttcc atcataatct agacgtttca aatgtcttaa
     2461 taatccaaga aaatcaacac aaccgctacc aaaaggaaca tcacgaaact ggcctgaata
     2521 ttcttttgtt acagggtaag tatcttttaa gtggatagca gtgatataat cgatcccttt
     2581 ctcaagttct cgagccggat tattttccgg ccatgcggac aaattgccta ggtctggata
     2641 tacttgtaga aaaggtgaat gaatctgttc tttgatttct aaaaattttg aaatggagtt
     2701 cataaatgga tcatccatga tttccacgga aagaatgatg ccatatttag aagcttcctt
     2761 tacgctttct tttattcctt cgataaagtc ttctctggaa ttcattgatt tctcttcata
     2821 ataaacatcg tatccagcta tttggattac tttgacgctt agattatgcg ccaaatggat
     2881 tgcttttttc attagttgtt tcgctgcttt tttcttttct ggatcagcag aaccaaacgg
     2941 aaatcgccga tgcgcactta aacaaatcga atggatacgt acaccggagc tgaacatctc
     3001 ttctctcagc tcagctatct tttcttcaga ccaatctaat cgtgctaacc gctcatccgt
     3061 ctcatcaatc gacatctcga taaaatcgaa gtccatttct tttacaaggg aaaaacgttc
     3121 tttccatgaa atatctttgg gtaacgcttt ttcataaatc ccaatttgag ccatttcctt
     3181 acccccagat acgaatgatc tcatctttga agttttgtgc ggccttcatc ggatcatttg
     3241 ctgctgtaat accacgacca gtgataaagg tatacacatc gatcccttca aataagtgta
     3301 atgtgtgtgg atttaatcca ccagttacag aaacattaaa gcctagttca atcagttttt
     3361 tgatcttcga taaatctttt tcaccccaag tttcaccaga taacagcgca tcacggcttt
     3421 gatggtagat tacctgccgg ataccgatgt tatgccaatc catggcttgt tcaaaagtcc
     3481 aattgccgta gagttctact tgcaattcac ctatttcttt ttgtgctgcc tccatagttg
     3541 gaatcgttgc acaacaaatg actgtcatcc agtctgctcc tgcttttgca acattactcg
     3601 ccactgttcc tccagcgtct gcacatttcg tatcagcgac tagtttttta tctgggaaca
     3661 tactgcgaat acagcgaatc gctttttgtc cttcctgtaa gcaaagaatt gtgcccactt
     3721 caataatatc tactacatca ccaactttca taacatctcc taatgcatgt tctaagtcat
     3781 tgtgatctaa cgcaatttgc aaattcggcc tgctcatctt agttccttcc tttacttgtt
     3841 tttttggtac cactttgttt cagcaaattt ttcactgatt tctttgactg acatcacatt
     3901 tttgataccg ataactgtaa ctcctttttt ctctgcctga gtaaacatat tcaaaaaatt
     3961 gactggcgta aaaactacat cgtattggcc agctgaactt tttccctctg aaatggcaca
     4021 atgatggatt ttggtaattg gtacgtttag ttctttcatt gctttttcca cacttctcat
     4081 catcatcaag cttgttcctg atccatttgc acacgatact aaaattctca tcttaatccc
     4141 tccattatga gtgttgtcta atcagatttt cttttatact ctctgctttt ctgcatatgt
     4201 gtcatagtct tctacaatca aaaaataacc atcaggattt ttccgatatt gtaactgcgg
     4261 aatagccaat aaaccaatga caactacggc aattccagca taacctagga ctttcatgat
     4321 tactgtaaac aatggccaag ctgttgccca gtcgaacatc ccgatataac caccgtatgt
     4381 tgacatccca atccatgtag cgaataatgc tgaaccgcct acttgtatga ttcctgaaag
     4441 aaacgggaac aggcaagcgg ctttgaatcc acctcggtta ttggcataaa ctgctataac
     4501 ggcattatca aagaacaacg gaatgaaacc ggctatgacg atcgttggtg atttcaataa
     4561 aatcagtaaa ccaatcatca aaaactgtcc caaagcacca aacaagaacc caatcgttac
     4621 cgcatttggc gaaccaaagc caaaagtagc agctacgtca attcctggaa cagctcctgg
     4681 taaaatcgtg tttgagattc cttggaaaga ttgagttagt tcatcgacga atgtccgaac
     4741 acctaattgc aagatcgcca gatataccgc aaaatttaaa gaagtcgtca taatgtagaa
     4801 taagaaactc tgcccttctt gcatgaattg cgcttgaatc aaataatctt tgcctagtac
     4861 aatcagaatg atcccaaaga aaaatagcat gagtaacgaa gtagcaacca tattctcatt
     4921 gaaaatcgac aagaatcctg gtaatttgat atcttcaatt tttttgtctt gcttgattga
     4981 tctatttttc ttttcatccc gctttttcat ccactcagct aattttgcaa atataaagat
     5041 tccgaacatt tgctgatgtg ccactgcaaa tccggcacct tctgtcaatt cctgcgtgat
     5101 tcctactgtt aagttagaag ctactgtcca ataacaacct aaaatcagtc ccataaaaat
     5161 caatgcttgt atcctaccta gatcaggaaa gcaaaacagt aaaatccaaa aagcggtagc
     5221 tgcttgttga acctgaacgt ttccggtagt gaaaacggaa cgcagtttcg tatatttctg
     5281 aaaacggact aataagatat ttaaaacaaa tgcaatcagt aataaaatca tcacatctcc
     5341 aaaggtacgg ccaaatgtct ccattaatcc agcatccaca gcattttgtc caaaataagg
     5401 atctgtaacc atggcatcca aattgaaccg ttcctttagt ccaaccaaga ttggccgaaa
     5461 attattgacc aaaccaccag agccaacagt caaaataaag taaccgaccg ttgcctttaa
     5521 gaatccagcg atgcattcat gaagcgggcg tttcagcaaa atatagccta ataacacgat
     5581 aaaaccaatc aggtatgctg gctgcgtcaa gatatttgtt gcaaaatacg tccaaatgct
     5641 taacaaaaat tcccccatct tttttctcct ctatcaatta attatattct gccatcacaa
     5701 cttggtaatc atcgatggag ttcgtagaga tgagacgctc aaccaatcca tctgtcatta
     5761 ataattcgga taatttttgg atattttcta gatgttcttc tggatctttt gctgcgagtg
     5821 taaaaaacaa actagccgtt ttctcctcat tctccccttc actttcaaaa cggattggct
     5881 gtttcaactt ggtaaatgaa atagctgttc caaaaacacc atcacttttt tctgaagaat
     5941 gcggcattgc tacttgaggt gcgatcacaa tataaggacc gtatttttct acacaatgga
     6001 tgatctcctc cacatacgtt tcatcaataa tttccttatc gatcaataca agacagcttt
     6061 cggcgatagc ttcacgccaa tccgtgattt ctttatctgg gtaatgaatc aattgatggt
     6121 caaaaaagta tttcagcaag aaactcccct tctttcttat aaaaacgacg gaaacggtgt
     6181 atcattttca atcgagaaga catcttcaaa tcctcgataa aaattaaact caagatcatc
     6241 tttgtttgtc ggatagatga atttcccgcc tacttgccag atataaggtt taaatccata
     6301 cttcaggcga tcctttttga atttccaaat ttcagtgatc tcttttggat cagccataaa
     6361 attagaccaa atatcgtagt ggacaggaat cactactttt gcattcaatg actccgccat
     6421 gcgcagcata tcaacagaag taactttgtc tgttatccct cgtgggtttt caccataggc
     6481 ccctaaacag acatcgattt tgtgttcatt accatgtttg gcaaataaat tggagtaatg
     6541 tgaatctcct gcatgataaa gattccctcc tgatgtttcg aataaatagt ttactgcaat
     6601 ttgatccatc tcctgaggca tttttccttt taatacctct tctggatctt cacaagtgac
     6661 caacgctgtc cgatcaaacg cttctaaagc tactacattg atgtctttga ttttgactgc
     6721 atcacctggc tttactgtaa tcgttttttc ttcaggaatt ccccattttt gccacgtgcg
     6781 aaccacttct tttggcccaa tgaatgttgc ttcaggacaa ttttgatgaa ctgctgcggc
     6841 tgtatggata tctagatgat cggaatggat atgtgtaacg attaaagcgt ctacattttt
     6901 tatcgcaaaa ggatcgatta cgaaaggctg agtacgtaaa ttaggctgca tattttgaac
     6961 gccactcata cgcatcatct gatggccttt tttcatcatg ccattcccat gactacgttt
     7021 tcccgtccca caccatagat cacataaaat attcgttcct tcatgacttt tcagccaaat
     7081 tcccgtacag cccagccacc acatagcaaa agttccagga tccactactt ctgtttcgat
     7141 ttcttcattc aagtatgtgc cccactcagg aaatgtttct actaaccact tttcttttgt
     7201 tacttcattg acagttttac tcatttgctt gctcccccca tatcgttgtt ccaacttaca
     7261 agtaaatatt aatgtgtatc agcatccatg aaaacccttt tatatgaccg tttgtttttt
     7321 atatgatttt ctttcagtaa tcaatgattg tttcagtata ataaagacag atatgaatgt
     7381 ttaaatgaac aactagtcaa ataaatccat ggatcaagat gtccctcagg aggcaaaagt
     7441 catgaaaaac tcacttgtca atattgaaaa acgtcaaaaa gaaattttgg aactgcttca
     7501 gcagactgaa caactatcaa ctttcgaatt agctgaatcc cttgatgtat ctattagtac
     7561 gatacgaaga gatttaaatt tattagaaga aaaaaacgag atcatacgca attacggtta
     7621 ttgttcttat aattacaaaa accagactga ttttgatcaa tctggtcctg aaaggattaa
     7681 acaagcgatt gccagagaag ctagtaaata cattaacgat tatgatacgc tgtttatcaa
     7741 ttctagctcg actgcgttga aaacactgga ttttcttcaa tctaaccatt tgacagtcat
     7801 tacaaacaac gtgaaaatca attactctcc gcacaaagat aattgtagtt atattttaac
     7861 tggtggagag atgcgctttc caaaagaagc actagtgggc gacatcgcat taaatacgat
     7921 ttcttcagtc aatgcagaca tttgtatcat tggctgtagc ggagtcgatg tagtacatgg
     7981 ggtcacaaca aagatattaa atgaatcaac gatcaacgaa atgatggtca acaagacaat
     8041 caaatgtaag attttagtcg ctgatcatcg aaaaatcgga ctgacatcaa agttcaagat
     8101 tgctgatatt tcagtctttg attatttgat cactgataaa ttttgttctg aaaaagtatt
     8161 agaggagatc agaaatacgg ggataaaaac cattcaagta gattcatttt aagaaccagg
     8221 acgtaaatat gtaaatagga ttatcaaaaa aatgaataac aaatttatcg ataaatgaaa
     8281 aacacgctac gctcaaattg ataaaggaat cccaagatgt atccatccaa tcgataccat
     8341 cataatctat tgcttctttt ataaccgtta tatgttgcct aaataggaat caaacaatat
     8401 cgaaaaaagg aggtatgaca aaagtcacac ctcctttttt cgaataaacg gtattcaaat
     8461 cagaacactg atttcacaac cactttattt caggtcgttt tcaatcacct ttaccgcgta
     8521 atcatttccg ttgaaacgcg ctattttttt tgcgtccata tatctttcta aataatcatc
     8581 ttctcctaat tgcttatggc aagaagcaat ttcataaagc aagtattcca atgcataagt
     8641 cgatttatgt ttcttattat actcaatacc cttctcacat aattggattg ctttatcata
     8701 atcctcgatt ttacggtaat aggtggcgat atcataatat acaatgcaga ccatcagatt
     8761 atcaaaatca gtctcactca atattttaag tgtacgctcc acataaactt tcgcaaaatc
     8821 taattctcct tttgacaagt agatcattgc cacgtttgca gctgcttgta agtgatggat
     8881 atctgtttcg gtcgtttgtt ccaacacttg attaaaataa aataatgctg catctggttt
     8941 tttcgcataa agatgatcaa gcagtccttg ataaaataaa atttgattta aaagatcaga
     9001 attacttact ttttcctgat cgatcgtttg taaaatttta taagattctt ggtattttga
     9061 tacagaattc aatgattgaa tctcatttat ttttttcctg attttgtctt cttctgtttc
     9121 tactaatata tcggagatct cgacattcag ctttttgcaa agcgcactta agatattgat
     9181 gcctggcgac atattcttgt tttcgatatt gctgacagtt gcttgttcac aaatgccttc
     9241 tgctaattgt acttgtgtta accccttttt ctttcggtat tcttttagta atgctccatc
     9301 tactttaatc aaaacaattt ccttcccttt aaaaaattat ttctccttta tatcatacac
     9361 aaaaaaaagt tctaaacaac caaaaatgtt tagaacagaa taaaaatttt aattttaaaa
     9421 taatattcaa attaaacaac tggcgtatct gctccttgat ttctttttgt gttatctgaa
     9481 taattatcgt ttacagttat aacacatagt gaaaattgtc atggcgagaa agtctaagaa
     9541 tatttttaaa cataactatt tctttcttac gtgtgtataa tgacgacagc acacctatca
     9601 attaatataa ttatt
//
LOCUS       DAYYCY010000098         9545 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000098 DAYYCY010000000
VERSION     DAYYCY010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9545
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.098"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            74..976
                     /gene="gnd"
                     /locus_tag="ACHAZB_001916"
     CDS             74..976
                     /gene="gnd"
                     /locus_tag="ACHAZB_001916"
                     /EC_number="1.1.1.343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydrogenase
                     (NAD(+)-dependent, decarboxylating)"
                     /protein_id="HFM8614556.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            978..1979
                     /locus_tag="ACHAZB_001917"
     CDS             978..1979
                     /locus_tag="ACHAZB_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-isomer specific 2-hydroxyacid dehydrogenase
                     family protein"
                     /protein_id="HFM8614557.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            1976..2260
                     /locus_tag="ACHAZB_001918"
     CDS             1976..2260
                     /locus_tag="ACHAZB_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFM8614558.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            2293..3090
                     /locus_tag="ACHAZB_001919"
     CDS             2293..3090
                     /locus_tag="ACHAZB_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFM8614559.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            3104..3937
                     /locus_tag="ACHAZB_001920"
     CDS             3104..3937
                     /locus_tag="ACHAZB_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM8614560.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGGFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            3955..4812
                     /locus_tag="ACHAZB_001921"
     CDS             3955..4812
                     /locus_tag="ACHAZB_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8614561.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            4834..5343
                     /locus_tag="ACHAZB_001922"
     CDS             4834..5343
                     /locus_tag="ACHAZB_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFM8614562.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            5380..5781
                     /locus_tag="ACHAZB_001923"
     CDS             5380..5781
                     /locus_tag="ACHAZB_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8614563.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            5893..6246
                     /locus_tag="ACHAZB_001924"
     CDS             5893..6246
                     /locus_tag="ACHAZB_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8614564.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            6280..6723
                     /locus_tag="ACHAZB_001925"
     CDS             6280..6723
                     /locus_tag="ACHAZB_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8614565.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            6740..7825
                     /gene="uxuA"
                     /locus_tag="ACHAZB_001926"
     CDS             6740..7825
                     /gene="uxuA"
                     /locus_tag="ACHAZB_001926"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFM8614566.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            7818..8531
                     /locus_tag="ACHAZB_001927"
     CDS             7818..8531
                     /locus_tag="ACHAZB_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8614567.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(8738..8887)
                     /locus_tag="ACHAZB_001928"
     CDS             complement(8738..8887)
                     /locus_tag="ACHAZB_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8614568.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            9283..9483
                     /locus_tag="ACHAZB_001929"
     CDS             9283..9483
                     /locus_tag="ACHAZB_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8614569.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         3208 a         1498 c         2116 g         2723 t
ORIGIN      
        1 aaatattttt gtaaaatcta tttacaaaaa aatttttttg tataatgagg gtataagaaa
       61 tggaggcgct atcatggaaa ttggtgtaat tggtttggga aaaatgggat tgaatttagc
      121 tttaaactta aaagagcacc atcacaaagt acaaggactc gacatttcaa gtacagctgt
      181 tagtcaggca agagaaaatg acattcctgc ttatcaagag atctctgaat ttctagctac
      241 ttttgagcag aaaaaaatca tctggttaat gcttcctgct ggagagataa ccgaaaacat
      301 gttaagaaaa ctttttccaa aactcggacc aggagacatt attattgaag gcggaaactc
      361 ttattataaa gattcaatcc gtcgagcaga agagtttcag aaaaatgaaa tcggttactt
      421 ggattgtggg acttctggag gaatcgaagg agcacgacat caagcctgct taatgattgg
      481 aggagataaa gaaacttttc tatccgtgga acaattattc aaagatgtag caattgaaag
      541 aggatatctg tatgctggtg catccggaag cggacacttc ttgaaaatga tccataatgg
      601 aatcgaatat ggaatgatgc aagcaatcgg tgaaggattc caacttgtac aaaaaagtga
      661 gtatgctttt gacttggaac aagtcgcacg tgtttggaat catggttcgg tgattcgcgg
      721 ctggctgatg gaaatcatgg aagatcagtt aaacgaacat cccaatttaa gtaattatcg
      781 gggaatcgtt tctgcatcag gtgaagcaaa atggacggtt gaaacagcgc tagaaatggc
      841 tgttccagtt ccagtaattg caactagttt gttcatgagg aatctttcac aggaagaaga
      901 tagtttttca gcaaaagtag tttcagcact aagaaatggg tttggcggac atgaaatagt
      961 tgaaaagggt gagtaaaatg ccagattatc atatagcaat cgttaattcc agcagtttcg
     1021 gaagagtctt tccacaccac ttggaaagat taaagtcagt tggagacgtt catttcttta
     1081 ctgttgatca agagattcca ggaaaagaac tagcggagtt acttaaagga ttcaatatca
     1141 ttattgcaag tgttacgcca ttttttcgac gagatttttt tgaaaacaaa gatgaactgc
     1201 tattgatttc ccgacatggt attggttaca acaatattga tttaaaagca gcaaaagaac
     1261 accagacaat cgtatctatc atacctgctt tagttgaacg aaatgctgta gcagaaaata
     1321 atataactaa tttgttgtcc atgcttcgac gcacgacaca agcacaagca agagtcaagg
     1381 acgatcgatg ggaagatcgg gcggagttcg tcggtaggac gctgtttaac aaaacagttg
     1441 gtgtgattgg tgcaggaaat accggaagcg gagtaatcga aattttgcgt aacggttttc
     1501 gctgtgatgt tctagcttat gatccttaca aatcagattt atacatcgaa agctttggtg
     1561 caaaaaaagt agaacttcaa gaattactgg aacaatcaga tatcatttgt ttatgtgcga
     1621 atttgacaga agagaactat cacatgattt ctgataaaga agtcgatcag atgaaagaaa
     1681 atgtttatct atctaatagt gcaagaggcg ctctagttga tgaagaagcg atgattaatg
     1741 gtctgaaaag cggaaagatc gcaggatatg ccacagatgt tttagaagaa gaaccgggac
     1801 gtcaagatca tccgtattta gcgtttgaac aagtagtgat tacgccacat atatcggcct
     1861 acacaaggga atgtttagaa gcaatgggtg aaaaatgtgt cagagatgtc gaacagatca
     1921 gtcagcacct tcttcctgaa cgttctgtcc agacaaagag caactatata aaaggatgat
     1981 gaagaaaatg gtgaacggtt taaaagtgaa aacaggccct caattttatt tatatgaaga
     2041 aggtggtatt tcaaaagttt ctgatctctt gaaatcatac ggcgcgaaaa gagtactggt
     2101 cacccacgga acggtatcat gggaaaaggc gctacctaaa ttagtatttt tgaatgacga
     2161 aacgattcaa tttttttatc atcgttattc tggagaatgt agttatgctg aagccagacg
     2221 aatagctaca atcatcaaaa aaatgaaatc gatttcgtag ttggcgttgg cggaggaaaa
     2281 ctgaccgatc tagtgggtta tagtgccaat cttgcaagaa tcgatttcgg agtcatccct
     2341 actttagcta gtaattgtgc tccatggaca cctcttgctg tcatgtataa ggagaatgga
     2401 caagcagaag ggaaaacaga acacttttac agacaagctg ctttttttat aacagatccc
     2461 caattagtga ttgattcccc tgttccatat ttcattgccg ggttagcaga tacactagca
     2521 aaatggtatg aatctgagct catcttagaa caagattttt tgcaagatga atcatttttg
     2581 aagctagcac aagatactgc gaagatctgc aaagaagaga ttttgaatca ttctgctaaa
     2641 gcaatccaag atatggcaca aagaaaactg acatcggagt tcaaacagct ttcagagatt
     2701 atctttgctg ttgctggttt agttggagga ttaggagata aatacgcaag aaatgcggca
     2761 gcacatgcta tgcatgacgc tatcagtaaa tatattccgg aaagccatcg ttttctacat
     2821 ggagaaaaag tagcgtatgg gattttctat caactggcac tagaaggtcg ctgggaaaaa
     2881 atagacgagt tatatccttt gtatcgggaa ttagaactgc cggtttctct agaacaaatg
     2941 ggattatttc cgaaagataa acagataata gataaaatgg ctgaatttat tgattcgaaa
     3001 gagaaagtgc atttgattcc tatagaggta acacagaaat cactgataca gtcaatgatg
     3061 gaactggaag gttatatcag aaagatctga aggaggagga cacatggaaa cattaagtgt
     3121 agtacaaagt ttgttgattg ctttatgggt agccgcggtt atgtcccgct ggttaggtgg
     3181 aggagccacg ttgaccttac gtttttctcc tttgatgact ggattagtcg tcgggttgat
     3241 catgggggaa gttgcacaag cgatgattgt tacagccgct ttgcaaatga tttatatggg
     3301 gggtttttct ccaggaggat caatgccagc tgaaccatct attgcagcag ctatcgcagt
     3361 accagtcgca ttattagggg atctgtcgcc agaagcagcg attgccgttg cggtgccagt
     3421 cggattgctc ggaagttact tgtatcagtt tcgttttttc atcaatactt ttttaggtaa
     3481 atatacagac aaagctgtag aagatttgaa tacaaaagct atggcccgat caattattct
     3541 ttatccaacg gttgcctcat ttttattatt cgttccgctt gttttcattg ctttatattt
     3601 aggggcgcca ttgatcgctg atgtaatcaa agctttagag ggaacagtcg ttatccacat
     3661 tcttgaagta gtaggtggag ggttagcggc aatcggtatc gcgacaactg tatacgttat
     3721 cggtcgaaaa gattatctgg tctttttctt ccttgcttat tttatgagtg ttgttttgaa
     3781 atctttagat attacgatgg ttacttatgc catttttggg attattatcg ctttgatctt
     3841 tgtccagtcg caaaaaggga aacaagtaca gcctgccaca agcaataaca attcagcatc
     3901 ttttgatgac gaagatgact atgatgacgg attttaattt aggaggaatg ggaaatggct
     3961 gaaataatgg ataatcaaac aggtgctcct gaagaaatca cgaaaaaaga tgtaacaaaa
     4021 gcctatctga gatggcattt tgcaaacgaa attcctcact cttttgaacg ttacttggcg
     4081 ccatcactac tatacggaat gatgccaatc ttgaaaaaac tttataaaga tgaagactct
     4141 ttaaaagcag catataaacg gcagttactg ttcttcaaca ctcaattaag ctggggtggc
     4201 ggtgtgatta ctggcctaat gtcttctatg gaacagcaaa gagcagaaga agagtatgaa
     4261 gaaaaagaga tcttgatgca ggatgacttg atgtataaca cgaaagcggg attgatgggg
     4321 gcactagcag gaatcggaga tgcaatcgat tcaggaaccg tccaatatat ttttatcgca
     4381 atcgcggtac cttgggcaca acagggaagt cctttaggcg cactttttcc ttttatctgc
     4441 tttgggttgt atcaggtttt actaggggta tttttcgcaa gacaagcttt cactatgggc
     4501 cgtaatgcta caggcctaat gcaaaacact ggtgttcaga cagcgattga aatgctatca
     4561 gttttagggt tgtttatgat gggggtgctt gcgggaaact atgtaaaagt agaatcttca
     4621 ttgaaatttg cgatatccgg acgtgagttt gttatccagg aaattcttga tcaaatcgtg
     4681 ccagggatat tacctttagc tgtcgtaatg ggtgtgtact tgttctatac gaaaaaagga
     4741 ttgaaagtca cacaagcatt attatggctg actggaatat taattgtgct agcaggaatc
     4801 ggtattttat aaaaagaagg gaacgtgaat aagatgggtg tagtcaattt agcgcgtgtg
     4861 gatgagcgtt tgatccatgg gcaagtaatg gtaacgttat ctcaaaaaag tggagtgaat
     4921 tccatttttg ttgtagacga agttgtagca aaagataaat ttatgagaga tctttacaaa
     4981 aatgcaggaa gccgtacagg acaaaaaaca attgtcatta caccagagaa agctaaattc
     5041 tattgggatg aatatcaatt taaagaatac aattgtattt tgatcgcaaa aacagtccaa
     5101 acgatttacg acttagtaaa acacggaatc cccatggaag aattgaatat tggagggatt
     5161 gctcaaaaaa atcctgatac agatctttta gtgacgaaat ctgtttattt aaataaatca
     5221 gatgctgaaa aattgaaaga aatgaatgaa caatatggcg taaagaatat ttattttcaa
     5281 gccactccat ctgcacctaa aacaggatta aaagatgtat tgaacaagtt tgatttgtca
     5341 taaacaaatg cttagaaaaa agataaagag ggcgaaaaaa tgaagaagaa cgagctgttt
     5401 cgtgattggg aatttcgtta tcgctatatt taccgtaagc gccgtacaaa aaagagtaaa
     5461 cagcgttttc tttcagcatt agtatcagat atttacagta tgagaacaga tgtgacagtc
     5521 attgcttacg atacgctagc ctaccgatca aaaaatattt atgttggaga tatcgaaaaa
     5581 gcagagaaag ttatctgtac ttattacgat acacccgttc atgcattagg aagttatttc
     5641 atgtttgatt ggaaagacca aaggaaaaaa acaatctact ctatattatt gtccttcatt
     5701 ttgttgttca gtttaggttg gtggggaatg atgatatata acaaaaatcc tcatcacgta
     5761 ttcgatctgc tatccgtata aacaggtatc actgtactag catttgggag ctactttttc
     5821 ttattgggaa aagcagctcg cggatggcct agcagacaaa cttttattcg aaatacttca
     5881 tccatattaa cgatgctaga aatgatccgt acgattgatg atccgagtgt agcgtatgcg
     5941 tttgttgatg aaggctgtta tggagaaaag ggcttagaca gcgtgagatc gggtatgaaa
     6001 aaagaggcta ttctttttta tctagactct gttggggcag atacaccgct acagttttcg
     6061 ggaaattatt tttcaaataa agaacagtgg ctgaaacaag ttgataagtt gaaagaaaaa
     6121 aacgtcaatt atatttttag tgcaagaaaa aagcaagcgc agttcttcta tttgaccaaa
     6181 acagatttac gagggaaaac atttaattgg caaaatgcca atcagattat tgctcttttt
     6241 cgataagtaa aaagtgcaaa acaaggagtg agaagactaa tgttggaaat catgatcgtt
     6301 acacatggta agttgagcga aggactgaaa gatgcggcgg aagtaattgt cgggacatcg
     6361 agccatattg aaacaatgtg cttagagtct ggggaggaca tccaaacatt cggagagaag
     6421 gtcaaagaaa aattaactat gatcaacaca gcagaaccca tgctagtatt tgttgatcta
     6481 attagcgcaa gtccgtacaa tcaagcgctc ttggcagtca atgaactgac tgaagaaaag
     6541 aaagcagcca cttatgtcat agggggcgtg aatctgccaa tgttgttaga agcaatcaat
     6601 catcagatgc ttcacacacc gatagaagaa gcggtggaac aaattgtcaa acaaggaaaa
     6661 gataatttaa ctgtttggca tgcttcagcg gaagaaaaaa aattagatga ggacgatttt
     6721 tagaaggaag gctgaaaaga tgaagtgggg atttagatgg tatggtgaaa aaggggattc
     6781 gatcccatta aaatatgttc gccaaatacc gggaataaat gggataattg gaactttgct
     6841 gactaaaatg cctggagatg tttgggagat ttcagaaatt gaggagttga aagcatctgt
     6901 tgagagccaa ggactggaac tgcttggaat tgaaagtgta gcgatccacg atgcaatcaa
     6961 ggcagggaca gatgaacgag attactatat tgataactat atacgaacaa tcaaaaactt
     7021 gtctcgatgt ggggttcgat tgatttgtta tagcttcaaa ccaatttttg gatgggcgaa
     7081 aactgatttg tttaatgaaa acaacgatgg aagctattct cttttatacg atcagaaaat
     7141 cgtcgatcag atggagccag gagaaatgta tcaattgata cacagccagt caaagggatt
     7201 caaattgccg ggttgggaag aagaacggtt gaaaaaattc gctcagctgg aaaaactgta
     7261 tgaaggaatg accgaagaag aattatttga aaatctgact tattttttag aaaaagtgat
     7321 tccagtgtgc gaaaaagcag atgtaaaaat ggctatccat ccagatgatc cgccatggga
     7381 actttttggt tttcctagag tgacgaaaaa tcttggcgac ttgaagaaaa ttctaaatgc
     7441 agtagattct ccatataatg gactaacgct gtgcacagga tctttagggg caaatccgga
     7501 aaacgattta gttgaaatca tccatgaggt aggttcgcgc atccattttg tccacttcag
     7561 aaacgtggag tttttaggag aaagaaagtt caaagaaacg gcacatccct ctacagaagg
     7621 ctcccttgat atgtatcata ttatgaaagc gttggtgcaa gaagggttcg acggtcctat
     7681 acgccctgat catggtagaa ccgtgtgggg tgaagtggct atgcctggat atggcctgta
     7741 tgatcgtgca atgggactca cttacatgca aggtctatat gaggcgataa gtaaggaaaa
     7801 accactggga gagcattatg aataaaatca agctcttcat ctttgatatg gatggccttt
     7861 tattagaaac tggacgttta gcttatcgtg cttatgtaaa atcagcacaa aagtatgatt
     7921 atgaaatgcg taaggaagtc tattatttac taactggcca aacagaaatg gcgataagag
     7981 aacaaatggg cattttgtac ggagaagatg ttccatatat caaatggcga gaagcaatca
     8041 atcaatataa agaaaaaatc gtgaaagaag ataagagagt atatacgaaa aaaggagcag
     8101 aagaaatttt atcttttgca aaagaaagag gaatccatac aatcgtggct tcgtctaata
     8161 cacgggtaaa agtagaaatg tatttaaaaa tggaaaatct atatgccttg tttgatcaaa
     8221 tcatttcagg ggatgatgta aaaaaaggaa agccggaacc agagatcttt ttgaaagctt
     8281 gttcaaaaat gaatattcct ccaagtgaag cacttgtatt tgaagactcg attgcaggga
     8341 tcgaagcagc aaggcgagcg ggcattttgt cttttctcat caaagatcat ttgatagggt
     8401 tgcccgatca taatgggaag cataagttga aagtagatgt aagtttatat gaatccaata
     8461 aagcagacta tgaattttat gacctgcatc aagcaaaacg atttttagaa agtaaagggt
     8521 tgtatctcta ataaagagga ctattttcta gtcctcttta tttattgtcc tgcacgactg
     8581 agggaacaga tgaaaaagaa gtaatttttt attttgaagt atctacctta tttgtagtga
     8641 acaatttaga acaaacacaa aacgcatcta ctgttatcta ccagtagatg cgtttaaaga
     8701 gaagaacgag aatcgtccat atgagggttt ccactcctta tgcagatttt tttaattctt
     8761 gcactgcttg ttcgatcgta acacctgtcg ctacatgatt ggtattttct ttgtcaaaaa
     8821 cgacaaatag attcaaatct gtatccaagg ttactcgata ttgtatatct ttgttataga
     8881 atgtcatagc cattctcctt tctttaaata cttgctgaaa ggcaacgaac gcgttgataa
     8941 cgaaattcca tttataagta taccaccaat tgaaaggttt tcatagttaa gaaattttaa
     9001 agtcaaaagt tgcagataaa agcaaataaa caagaaaaca aaaagtgaat caatcgcggt
     9061 tattcaactt tttcttcgat tcgaagacaa tagaagacaa tgaattttat atttcattgc
     9121 attatccttg tgttattaga aaaagcatga tatattaaat gagtaatcta ttttgaaacg
     9181 taatttgagc gatatatcta catagataaa actccttttg cctatcaaat atttattgca
     9241 acgaaaaaac gtacattgat acgtatcagg aggaatttat ttatgaataa cggtacagta
     9301 aaatggttca acgcagacaa aggttttggc tttatcactg gtgaagatgg aaatgatgta
     9361 tttgcacatt tttcagctat ccaaggtgac ggcttcaaaa ctttagaaga aggccaagca
     9421 gtgtcttacg atgttgaaga tggtcaacgt ggccctcaag caacaaacat tgttaaagct
     9481 taataaaaat tgacttttaa ccgtcttttc gaaagaaaag acggtttttt tgtacgaaaa
     9541 aattc
//
LOCUS       DAYYCY010000099         9427 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000099 DAYYCY010000000
VERSION     DAYYCY010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9427
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.099"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            35..1084
                     /gene="trpX"
                     /locus_tag="ACHAZB_001930"
     CDS             35..1084
                     /gene="trpX"
                     /locus_tag="ACHAZB_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8614570.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            1081..1962
                     /locus_tag="ACHAZB_001931"
     CDS             1081..1962
                     /locus_tag="ACHAZB_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8614571.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            1965..2735
                     /locus_tag="ACHAZB_001932"
     CDS             1965..2735
                     /locus_tag="ACHAZB_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8614572.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            3014..3868
                     /gene="aroE"
                     /locus_tag="ACHAZB_001933"
     CDS             3014..3868
                     /gene="aroE"
                     /locus_tag="ACHAZB_001933"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFM8614573.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            4046..5209
                     /locus_tag="ACHAZB_001934"
     CDS             4046..5209
                     /locus_tag="ACHAZB_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFM8614574.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            5326..5736
                     /locus_tag="ACHAZB_001935"
     CDS             5326..5736
                     /locus_tag="ACHAZB_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8614575.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            5910..8138
                     /gene="topB"
                     /locus_tag="ACHAZB_001936"
     CDS             5910..8138
                     /gene="topB"
                     /locus_tag="ACHAZB_001936"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFM8614576.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            8309..8593
                     /locus_tag="ACHAZB_001937"
     CDS             8309..8593
                     /locus_tag="ACHAZB_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8614577.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            8753..8989
                     /locus_tag="ACHAZB_001938"
     CDS             8753..8989
                     /locus_tag="ACHAZB_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFM8614578.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            8986..9384
                     /locus_tag="ACHAZB_001939"
     CDS             8986..9384
                     /locus_tag="ACHAZB_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFM8614579.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         3282 a         1605 c         2007 g         2533 t
ORIGIN      
        1 taaataaaaa gattgacttt gcaggaggaa aaaaatgaaa aacaaacgat taataacagt
       61 tgtagtaatt attttagttt atttagcagg tgcatttatt tatggaaaag cctcaggcga
      121 aaaagaaagt acttcgacga accagacaaa aaaaacaaca gttgttgggg tattgcaata
      181 cgtgagccat ccggcattag atgagattta tcgaggaatc aaagatggtc tgaaagaaga
      241 aggattaaaa gaaggagaaa atcttgagat caaatttcaa aatggacaag ccgatcagag
      301 caagctagcc acaatgagtc aacagctagt acaatctgat ccagatgcct tgattggaat
      361 cgcaacacct gccgctcaat cattagcaaa cacgacttca tcgatcccag ttgtgttagg
      421 tgctgtaacg gatcctgtgg gggctggatt agtcgcttct ttagagaaac ctggtggtaa
      481 tgtaacaggt gtttctgatc agccgcctgt tacttcacaa atcaagcttg cttctgattt
      541 gttgccaaat gcaaaaaaag tcggaatctt gtatgcttct tcagaggata attcaaaata
      601 ccaagtagca caagcagaag aagctgcaaa aaaagaaggg ttagaatcag taaaatatgc
      661 ggtgccttcc actaatgaga ttgcacaaac ggttcaagtg atgagccgac aagtcgattt
      721 tatctatgtt ccattagata ataccattgc caatgcgatg caaacagtgg taaaagaagc
      781 aaacaaagca aatatccccg tcattccatc tgtggataca atggtggaac aaggtggact
      841 agcaacaatc gggatcaatc aatatcaatt agggcttcaa agcggtaaga tggcggcaga
      901 acttgcgagt ggaaaagaaa aaccggaaac gacgcctgtt tatacatttg atcaagggga
      961 tacagtgatc aatcaatctc aagctgatcg cttaggaatc attgttcccc aatcgatgaa
     1021 ggaaaaagcc aaaatcatta cagatgactc gcagcaagaa acatctaagg aggatgacaa
     1081 atgatcgtat cagctatttc acaaggcatg ctttgggcaa tattaggatt agggattttc
     1141 atgacttatc ggatcttaga ctttcctgat atgacgacag aagggtcctt cccattaggt
     1201 gctgctgtct gtgtgacagc tattactcat ggggtagcgc cgattgtagc tacattatta
     1261 ggcgtaggtg ctggcatgat agcaggactc gttacggggt tactttatac aaaaggaaag
     1321 ataccggtta ttttagcagg gattttagtg atgtcaggat tgaactcagt tattttgttt
     1381 gtgatgcgaa caccaaatct ttctttattg aatcatccga ttctacaaga cagcttccat
     1441 cgctttgggt tacctgatta ctttgacaca gtccttcttg gcttcatctc tttgggcatc
     1501 ttgattgctg tactgctatt cttcttcaat acagatttgg gtcaaggcta tattgcgaca
     1561 ggagacaacg aaacgatggc tcggtcgtta gggataaaaa ccgatcgtat gaaaatttta
     1621 ggtttaactt tgtcgaacgg tgtcattgca ttatccggtg cgctgattgc ccagaacgac
     1681 ggatatgcag atgtaaataa agggattggc gtaatcgtca tcggtcttgc ttccatcatc
     1741 atcggtgaag tgatcttcca agaattgacg ctagcagaaa gattgattgc tattgttgtc
     1801 ggcagtatca tttatcagct attgatttta gtagtaatca aactagggtt tgacacgact
     1861 tatctgaagt tgttctccgc tatcatctta gctatttgtc tgatgatccc gcaattgaag
     1921 aaagcactga acttaaaaac gggttttgag aaggaggcat agtaatgact gatatattga
     1981 caatcaaaaa tggcagaaaa gtcgtaaaca atggaatgaa tgaagagaaa gtactattgg
     2041 atgaaattaa ccttacaatc agagaagggg acttcatcac tgtccttggc gggaatggtg
     2101 ctggaaaaag cacactgttc aatacgatcg ctggtacgct tcaactcagt caaggatcga
     2161 tctcttttaa agatcagctg atcacaaaag attcagaaga aaaaagagca cgattccttt
     2221 ctcgagtttt ccaagacccc aagatgggaa cggctcctcg gatgacagta gcagaaaatt
     2281 tgctacttgc acaaaaaaga ggtcaaaaac ggaaattccg tttacgtaac ttgaaaaatc
     2341 aaaaagaaga ttttttcaga ctatgtcaag aagtcggtaa tggcctagag caacacttgg
     2401 atactccagc gggtgaatta tctggtggtc aacgccaagc attaagcttg ctgatggcga
     2461 cgatcacagc tcctgagtta ctattattag atgaacatac tgcagcttta gatcctaaaa
     2521 cagctaaagc attaatgcga ttaacaaatc agcggatcaa tgaacaaaaa ttgacttgtc
     2581 tcatgatcac gcatcgaatg gaagacgcct tgaaatacgg aaatcgtctg atcgtactcc
     2641 aaaaagggaa aatcgttcaa gatatttctg aaaaagaaaa gcaacatttg acaatgggcg
     2701 accttttgca gttctttgaa gaagtagtgg attgatttcg acagaaaaat tcctttcagt
     2761 agaaggactt gaaaactcat tgacatcttc ctttgattca tcctatactt aaaacaataa
     2821 acaattaaac atatttgggt gttttgtagg atcacgtagt caatgatttt aacaattaaa
     2881 tagaatattc tttgctctga caagtaaacg atgagaaaag aatttgctac tttgccacct
     2941 agacgtttcg tactttttcg tatcaaacgc taagtggctt tttttattga agtaagaaca
     3001 aaggaggaat aagatgattt ctggacaaac caaattagct gggctctttg ctgatccggc
     3061 ttctcacagt ttatccccat tgatgcacaa tactgctttt gaagcaaaca acatcgatgc
     3121 ggtgtattta gctttccaag taaatccaat gaatttgaag caagcagtcg aaagtattcg
     3181 aacttttgat atgttaggtg taaatctttc gatgccgaat aaaacagcgg ttattcctta
     3241 tctggatgag gtaagccagg aagcgcaatt gatcggagca gtgaatacga tcgtgcacga
     3301 aaagggtcgt ttgattggct ataataccga tggtgcgggc ttcatgcaat ccgtgcaaga
     3361 agctgggatt tcgatcaaaa agaaaaaaat gactctactg ggagctggag gtgcagcaaa
     3421 ggcagtcact gttcaagctg cgttagacgg cgctgaggaa atcgtagttt ataaacgaaa
     3481 caaccaaaca ttcgctcaag taaaagaaac attcgaacaa ctcagctatc gaacttcttg
     3541 taagataaaa gttcgtgact ttgccgatac gcatcaatta agaaaagact tgaaagaaag
     3601 ctgtcttcta gtcaatgcaa cagatatcgg gatgggaaag aaaatcaatc agtcaccgct
     3661 cttagatata agcatgatgc atgagaatct agcggttttt gatttgattt atgcacctag
     3721 agaaacacgt ttattaaaag aagcaaaaaa agtaggagca aaaggataca atggacttgg
     3781 catgttgatc taccaaggag caatcgcgtt tgaattatgg acaaaggaaa agatgccgat
     3841 ccatgtgatt gaaaagctgt ttgattaaaa aaggataata gataaagatc ttttattctc
     3901 gcataaacgg tctcaagaaa agtaatcctt tatgtatcga agcatttatt tcgttatttt
     3961 ttattaaatt atcaaaaaat gataggcttt tgtcgattgg ttatcgtatc ctttatttat
     4021 cagtaagaaa cgaggaggaa ccacaatgag tcggatggat cgctataagg acatccataa
     4081 gaaggcgaaa cctctgaagc gggatacggg gagtattttt gctagaagag aacgaaaacg
     4141 aacgcgtatg gaaaatgagg aagatgctac ccgtatttat gaaagagagc agcattcatt
     4201 tgaacatttg aaccaatacg agcaacagat atacagagat gcaccaagag cagaagaaca
     4261 acaaaaaaat aaaaaatttt tcaatcggca aaagaagaac aaaccgttaa aacctcgaag
     4321 aaagcgatcg tggaaaaaaa tcattctatc gattttgctt gttttgatcc tttattcagg
     4381 tatctcattt tttattggta aatatgtagc tgaacatgac acaagcttag aggcagcgac
     4441 ttcagaaacc tttcatgggc aaaaagcgac gaatggtgcg cataatattt tgatccttgg
     4501 aagtgataca cgcggagaag atgccgggcg agccgatacg atcatggtct tacagttgga
     4561 tggtccttcc cataagccga aactgatttc ttttatgcgt gacagcttcg ttacgatccc
     4621 aggggtagga caaaataaga tcaattcagc ttatgcatac ggaggagccg atctagtgcg
     4681 tcaaacttta gtagaaaatt ttggaatcga ttgtcaatac tatgcgaaag tcgatttcaa
     4741 atcgtttgaa aaagtgatcg atgccttatt catgaacgga gtgaagatcg atgcagaaaa
     4801 agatctgaat ctggatggtg tagatattaa aaaaggggtc cagaagatgg atggacatgt
     4861 tcttttgcag tacgcacgtt tccgaatgga tgaacaagga gactttggcc gtgtcagacg
     4921 ccaacagcaa gtaatgaacg ctattttcag ccaattgaag aatccattga atctgatccg
     4981 tgcaccttat gcagctggta aagctttagg atatacatcc acagatgtca ctgcttggtt
     5041 cttgacgaaa aacctctttt cgattgcacg aggagctggt ggcattgatc gaataagtgt
     5101 gccagtagac ggttcgtgga attatgggca aagtgattat gctggcagta ttcttgaaat
     5161 tgataatcaa atgaatcaat ctgctattac agattttttg aataaatgat cataaaaaag
     5221 atcaagaaga agctgcgcaa ggcttttgtc atgaatcaat caaacgattc actcgtaaaa
     5281 aacgctttct tatggtagat ttaaccaaga gaggagctgt ttacattgga tatctatctg
     5341 aacttcaaaa atgagtcgcg tgaagccatc gctttttatg aaaaagtttt tcagactgaa
     5401 gcatcaggta tcaccacata cggtgaaatg cctggaatag aacaaatgaa cttgccagaa
     5461 gaagcaaaaa atctaatcac gaatgcacgg ctgaacattt atgatacatt agttatgttt
     5521 gcagatacgc cagaaggaat tggtcctgaa ttagttgtgg gaaataatgt ttctttagtc
     5581 atccagacaa gaaatgaaga agaaacgaag cgcctatttg aaagcttagc tgagggtggt
     5641 aagattgaga tgccactgga tgctgttccg tgggctcagt tgtatgggat ggtaactgat
     5701 cgatttggca tcaactggca gattaaccaa gagtgacagc taaagatgaa tattttctgg
     5761 aaacatcaag cagcaaacgg atcatcgttt gtttgcttga tgtttttgtg tctatttcga
     5821 ctatacggta tctttttttt attgccagat gcgaaaatat gttccataat agagaagtcc
     5881 gcaaaaaatt aagtaacgga ggtcagaaga tgaagacggt gatactagct gaaaaacctt
     5941 cacaagcaaa agcctatgcc gaaaccttcc aacagcaaaa gcggcatgaa gggtattatg
     6001 aaatcagcga tccattgttt gctggtgaag taaccatcac ttatggtttt ggacatttgg
     6061 tagatatggt tgctccagga gcttatgaag agcgttgggc aaaatggtct ttagaaaatt
     6121 tgcctatttt cccagaaacc ttccgttacg aagttccaat ggacaaacgt gcacaatttt
     6181 cgatcgtaaa acgagaattg cagtcagccg ataccatcat tattgcaaca gatggtgatc
     6241 gagaaggtga agccatcgcg tggtcgatta ttcgacaagc aaacgcattt tctaaggaaa
     6301 agaactattt acgtttatgg atcaattctc ttgaaaaaga agcaatctat gaaggtttta
     6361 agaatctcca acctggaaag aactattttc caaaatataa agaagcgcaa gcaaggcaga
     6421 acgctgattg gctgattgga atgaatggaa gtccattata tagcttgcta cttcaacgaa
     6481 aagggattga tggcagtttt tctcttggtc gtgtacaaac accgacactc tatatgattt
     6541 atcagctgca agaagaaatc aaacacttca aaaaagaaac gtactttgaa ggagaaggtt
     6601 taattacgac aaacaaaggg cagttttctg gaaaaatcgt accaaaaaaa gcatttaaaa
     6661 cccaagaaga gttaacgaag gaaatagaaa aacttggagc gcatttaggt aagcaaaacg
     6721 gacggattct tgaagttacg aaaaaggaaa agaggttgca tagtccacgt ttattttcgc
     6781 tatctagttt gcagacaaag atgaatcagc taatgaaagc aagtgccaaa gctacacttg
     6841 atgctgctca aggattgtac gaaagcaagt ttctaagcta tccaagaacg gattcctttt
     6901 acattacaga aaatgagcat cagtatctgg ttaaacatct gcaaaattat aaaaaatttt
     6961 taggaataga agaagtacag acaactgaga tcaaagcaaa gaaacgatat gtcgatgcga
     7021 aaaaagttca agaacatcat gcaattattc cgacaaagac gattccgact cctgcacgct
     7081 ttgcgaagct ttccaaatta cagcaagcaa tctatttgca agttttgcgc actactgtgg
     7141 ctatgtttgc tgcagattat atctacgaag agacgaccgt aatgacgggc gtacagcaat
     7201 taaagctaaa aagcatcgga aagatcccat tacagcaagg ttggcaggaa atcttacgca
     7261 tcaagtcaaa aaagaaagaa gctcagactc ttcctcctat cgtagaaaat gaagaagcag
     7321 aagtagaact gaaaagtatg gagaaagaaa cacaacctcc aaaaccctat accgaaggaa
     7381 cgttgatcac agcaatgaaa actgcgggaa aaacgctgga taatgatgaa gcgcaagaaa
     7441 ttctcaaaga agtagaagga atagggacag aagctacacg agccaatatc attgaaaatc
     7501 ttaagcagaa gcattacatc gaagtcaata aaaacgaaat aactgttacg ccaaaaggaa
     7561 tcactctttg taaggcagta gccaatgaac cattactgac gagcgcagaa atgactgcgc
     7621 gctgggaagg gtacttgaga aaaattggta aacaagaggg gacaccatct gtttttttag
     7681 agaatatcaa aaaattcatc ctacatttat tggcagaagt tcctaatcaa atcgacaagg
     7741 tagacttttc agatgagatt agcgggacac gtcagataaa agcgatggag aagaaaaacg
     7801 atcaattagg aagatgtcca aaatgtaaaa agggaatagt catgctttat cctaaaatcg
     7861 ctacatgctt gaatccggaa tgcgacttca aattgtggcc aacagtagct aaaaagaagc
     7921 tgactaaaac gaatctcaga gatttgcttt caaaaggaaa gacaagtaaa gttgtcaaag
     7981 gttttactgg taaaaaaggg aaattcgatg cagttttagt attaaaagaa gatatgacaa
     8041 tcggtttttc ttttccatgg atcgaaaaca gtggaacaaa agcagttgat ggaaacaaag
     8101 aaacatcaag aaaaaataat gaaaacgaag tagagtaaat gaaaacaatg aaatagcgga
     8161 atatttattc cgaaaagtga gaatataaat accaataaaa ctaaaaaata tcagtgaatc
     8221 aagcaaaaac gttggggtag gctgatttta tatagatagc aaggagtttt tttatttgaa
     8281 aaaagtgcat tcatttttaa attatcttat gggagcaatc acgattgtgg ctgtgtacca
     8341 agcatttcta aagtatcctg attacgggac gaagttgttg tcattgacgc acaatcaaac
     8401 agcatattca ccaacgttga ttttatcgac tttgactgca ggagctaaaa gcgctactta
     8461 tttttttcat aaaacgacaa gcatgctaaa gcgaaagaaa cagcgaaaac aattgttggt
     8521 ggtggtagcg gctatgatca ttatttattt tctaccagtt attacacaag tatttcatca
     8581 atattttggt taagaatacg agagtttgtg acaaaagtca tgtcacaaac ttttttcttt
     8641 gtcatttttt cgtaagaaaa agcaaaataa aagtatatac attgattaaa agtatatact
     8701 tggatataat gtacttacta tagaatgaga atggtataga gggtggcgaa atatgaaaaa
     8761 aatgaaaaag tataaaatta gaaaatctgg caatagcgat gtaacaacta tcccaccaga
     8821 agtaaaagaa ttcatgggtg tgcagacagg tgacgcaatc agttatgttt ttcaatctga
     8881 tggttctgta cgaatgatca aagcacaaga agaacctgat attgattcat tagttgattc
     8941 gatcatgaat caatatgaag acgcgttaaa agatttggtt gatttatgag gtatctttcg
     9001 actaaagaaa ttatcaaaat caacgcgaaa gttattttac gtcactcgcc aggcgaaatg
     9061 atcggtgtaa aagatgcaaa tgcattagat atggccgtca aacagccttc acaagcagta
     9121 ttcaatcaag aactctatcc tgaaatctat gaaaaagcgg cgattttagc aattaattta
     9181 gctaaaaaac atccttttca taatggcaat aaacgaactg ctcttgttgc catgttgttg
     9241 tttcttcaat tgaataattg tccagttgct ttttctcgtc aagaagctgt tgattttatc
     9301 ataatgatta ccactagttc attggatttc gattcactaa agtccaagat aaccagttat
     9361 ttacgacaaa ccgcaataaa atgaacgaat taaaaacttt atttctcaat tagatcagca
     9421 tgtcatt
//
LOCUS       DAYYCY010000100         9395 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000100 DAYYCY010000000
VERSION     DAYYCY010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9395
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.100"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            179..2107
                     /locus_tag="ACHAZB_001940"
     CDS             179..2107
                     /locus_tag="ACHAZB_001940"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM8614580.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEEAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            2245..2700
                     /locus_tag="ACHAZB_001941"
     CDS             2245..2700
                     /locus_tag="ACHAZB_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFM8614581.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            2780..2908
                     /locus_tag="ACHAZB_001942"
     CDS             2780..2908
                     /locus_tag="ACHAZB_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8614582.1"
                     /translation="MFEKLKYFIGTIGWFLLAVLLILGLAYLLFPDLIQNVFDTAF"
     gene            3170..3439
                     /locus_tag="ACHAZB_001943"
     CDS             3170..3439
                     /locus_tag="ACHAZB_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8614583.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            3839..4093
                     /gene="rpsT"
                     /locus_tag="ACHAZB_001944"
     CDS             3839..4093
                     /gene="rpsT"
                     /locus_tag="ACHAZB_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFM8614584.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(4182..5228)
                     /gene="holA"
                     /locus_tag="ACHAZB_001945"
     CDS             complement(4182..5228)
                     /gene="holA"
                     /locus_tag="ACHAZB_001945"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFM8614585.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(5266..7539)
                     /locus_tag="ACHAZB_001946"
     CDS             complement(5266..7539)
                     /locus_tag="ACHAZB_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFM8614586.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            complement(7532..8071)
                     /locus_tag="ACHAZB_001947"
     CDS             complement(7532..8071)
                     /locus_tag="ACHAZB_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HFM8614587.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            complement(8207..8425)
                     /locus_tag="ACHAZB_001948"
     CDS             complement(8207..8425)
                     /locus_tag="ACHAZB_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFM8614588.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            complement(8422..8667)
                     /locus_tag="ACHAZB_001949"
     CDS             complement(8422..8667)
                     /locus_tag="ACHAZB_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFM8614589.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            complement(8783..>9395)
                     /locus_tag="ACHAZB_001950"
     CDS             complement(8783..>9395)
                     /locus_tag="ACHAZB_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFM8614590.1"
                     /translation="VLIFCIASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEG
                     NDVEEDARPKVMYTDIKGSVKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAK
                     IEDQQVLYIPAVGEELPEHLDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGS
                     VKAQEIIRFREENGSFQKVEDLQEISGIGEKTVEKLKNFVTIK"
BASE COUNT         2910 a         1829 c         1638 g         3018 t
ORIGIN      
        1 tcttatttga gaaatgttct ggttttttat tttctttaat aataatcatt ataaataata
       61 aaaacaacat caagcttaat caccaaatga taaccattat cgttctcatt tagctgttat
      121 tattatcaat tacattttta ttatgctatc cttgttttga caaggaggtc taataacgat
      181 gactaatcag aaaaaagctt ttgcagcaac aatcggctgc cttttattta tgatcttcgg
      241 ttttatcttc gaccgattct ctcttaccat caccccactt ttttatatca gtgccattat
      301 catcggtgga tataaacaaa catcggaagg cttgaaagaa ttgtggaatg atcggacact
      361 gaatgtggat ttattgatgg ctttagctgc tattggtgcc tgtttgatcg gtaattactt
      421 tgaagaagca atgttgacat tcatcttttg tttaagcggt gcattagagg aatacacaac
      481 taataaaagt caaaaagaaa ttactgcttt gatgaatctt caaccacaaa aagcccaatt
      541 attaaaagaa aatggacaaa tgcaagaagt agatgtttct caactaaagg tggataactt
      601 ggtatttgtc gctaaaggag cagctgttcc gatagatggc tttttagaaa gcacacaggc
      661 aacgatcgat gagtctgcat taagcggtga atctgtgcct gtagaaaaat atttaggcga
      721 tgagatgtat gcaggaacgc tgaatcaagg aaatcccatc acgattcgag taacaaaaac
      781 gagttccgaa actgtctttg ctaagattat ccaattagta gaagaagcgc agaatacgcc
      841 aactcaaaca gcttcattta tcgaacgaat cgaaaataat tatgtaaaat tgattttgct
      901 tgctgttcct ctaatgatcc ttctccccca ctttttccta ggttggagtt gggatgaaag
      961 cttttatcga ggaatggtat tattagttgt ggcttctcct tgtgcgcttg tcgcatctgc
     1021 tacaccagct tctctagctg ctctttcaaa ttctgcaaaa aatgggattt taatcaaagg
     1081 cggtatccat ttagaaaagc tttctgaatt aaaagcgatt gcctttgata aaacaggaac
     1141 gctcactaaa ggaaaaccag ttgtaactga cagctttttc tttgatgaaa aggatttggc
     1201 acaacaactt cttgttgcta tggaacaaaa aacgacgcat ccacttgctc atgcgatttt
     1261 gcaatatttc gattgcacaa tacctgaaga aatccaatac ttaccaatag aagaaattac
     1321 tggttttgga ttacaaacta cctatttagg tgcgcaatgg aaagttggca agcacgcata
     1381 tgatcctgag acgatgataa tcagcaaaga gattgcggaa atgatcgaac gtttggaaaa
     1441 tcaaggcaaa accgtgattt atctttctaa agatcagcag ttaattgctg ttttaggtct
     1501 gctagatatt ccaaaagcaa atacccaaca agtaatttcc tactttaaat cccaaaacat
     1561 ccatacaagt atgataaccg gcgaccattc tggcacagca aaggccattg ctgaacaagt
     1621 agggattgaa cattactatg caagctgtac tccggaagaa aaaacacaac tagtcaaaaa
     1681 agagaatgaa ctttaccaag taaatgcgat ggttggtgat ggagtcaatg atgcgcctgc
     1741 tttagcagcc gcttctttag gtgtcgctat ggggcaaggg acagatgccg ctatggatat
     1801 cgcagacatc gtcttaatga aaagcgactt aaataaattt gtctacagtc atcggctatc
     1861 tttaaaaatg cggcggatca tcaaacaaaa tattattttt tcgattcttg ttattctctt
     1921 gttgattgct tctaacttcc tgcaattttt aaatctacct cttggagttg taggacacga
     1981 aggaagcaca atattggtta ttctgaatgg cttgcgttta ctaagacctt tacctgatct
     2041 ttctgattct tcaaaaaaat gtaagtcttg tccactatac aaagcgcggc tgtcttctgt
     2101 caaataagca aagtgttttt ttatgatttt gtttatttta tgaaaaaaac agcttatgaa
     2161 taggtcttga aggatgaatt cctacttttg taactttcta aaaacatgtt atgattgttt
     2221 cgaagaaagt gaggaatata tttcatggaa agttggcttt ttttaggaat tattttactt
     2281 gtagcaattt tagctaaaaa tcaaagtctg atgatagcgg cagcttttgt tttgattatg
     2341 aagctcatac cagcctcccg ttcctttttc ccttggcttc aaggcaaagg aatcaatatt
     2401 ggggtgacga tcatcacagc ggctattcta atccctatcg caacaggaga aatcggtcta
     2461 cgtgatctat tagaagcctt taaatcccct attggctggg tagctgtttt ttgcggtggg
     2521 ctagtggctg tcttatcttc caagggtgta ggattgattg cttctgatcc acaaatcact
     2581 gttgcacttg ttttcggcac gattttaggt gttgtcttct taagaggaat cgccgccgga
     2641 ccggtcattg cttctggtat cacttactgt atcttacaag tatttgcttt aggaaaatag
     2701 aacaaatttt gtctatttta ggagattctt aacaccccca atttactgat ccgtgatata
     2761 ctgacaacga ggtgatacaa tgttcgaaaa attaaagtat ttcataggaa caatcggctg
     2821 gtttcttcta gcggtacttt tgattctggg cttagcttat ctattatttc ctgatttgat
     2881 acaaaatgtg tttgatactg ctttttgatt gacctattaa aacgatatag gggatgctcc
     2941 tgcgatgcag gggctttttt taatcaatgt tttgtaataa tttaggccgt gccaatgaga
     3001 cttcctatag aattcgatgc tcttttttct ttttggctct tctgctcttt tttgttatac
     3061 tagattacag tggacaaaag aaagaatgat cgcatccact tcaacgaata tcaaaatgga
     3121 agaattagtg cctgttttca ggttactttg aaaagaaagg aagacgcttg tggaaacttt
     3181 tgaaggtctt ttaattgata cgtacgtttt acaagaacag ccaatgatgc tgactgggat
     3241 caacttgcag cttgaggacc gtgtgatcaa tggcctgctt aaagatcgct atttggcaaa
     3301 atatatctta cgtttgcccc taaataaata tgtgctggaa gtaagtggca aatggaaccg
     3361 tcggaaccag ttggtcataa aaaaaatgtt gataaaaaat atcgatgaat acattaaaat
     3421 aatcggtaca ccagaatagc ctttactgat caaaggatac tggttataaa ataactgcag
     3481 tttcttaata gaaaaacaaa gaacaacagg gaatcgtatc ccttttcaaa aaaaactttg
     3541 caaacggttc tctttgttat ataataactt agtaccaaac attcccattc aaactttgtt
     3601 taaatagata aataaaaatc tagtttggtt tgacgctatt gcctcccaca atagcgtctt
     3661 tttgattttt ttcagcttgt actatccgtt tttactgaaa aaaaatccta ataaaagact
     3721 gttaaggaaa aaaacttgtc atgttcatat tgcaaagcac gctgccttta ggtataataa
     3781 tgaatgttga attatgcggt aacacgcgta agaacttaag ttatcggagg tgaaactaat
     3841 gccaaacatt gaatctgcaa tcaaacgtgt tcgtacaagc gaaaacgcta atgtgaaaaa
     3901 ttcatctcaa acaagcgcta tgcgtactgc aatcaaaaaa tttgaagatg ctgtagcttc
     3961 aggtgctgac aacgtagatg cgttatataa agaagctgtt aaagctattg atatggctga
     4021 atcaaaaggt ctaatccaca aaaacaaagc taaccgcgac aaatctcgtt taagcaaaaa
     4081 aatcgctaaa taaaagaatc ggcttcggcc gattttttta tttgtcaaaa aaaggacaaa
     4141 aacaaacgac cttcccgcat gctttgccct cgtctttctt cttatcgatt tcttaacaac
     4201 tgccctgtgc gcaaaacgaa tagttgaaaa cttaattctt tttcgatttg tcccgttttg
     4261 atttgataat ccatctcaac taattgatca aacaactgta ccagcaaatg ttcatcgaat
     4321 ttttttactt cctgcatggc aagttttaca cgatatggat ggattttcaa tgtttcagca
     4381 atatttgcct gctgataacc aatctttaca agaaattttg tctgtaaaaa taaacgtaat
     4441 tgagaaagca aaatcgcatt aattttgatt gtttcttccc cttgcataac taaatcttcg
     4501 tataaacgca aagcttgttc cgtcttccct tttaaaatat attgggtcat gtcaaaaata
     4561 ttatgttcta acgttttagg aactaattct tctacttctc ttaccgtgat ttgctgttga
     4621 ttttgtccat aaaggaccaa tttctgtaac tctctcatca ttttggataa atctaagtcg
     4681 gtcaacctca agaataaatc aattgcactt cgttccattt tgattgagga attctctaag
     4741 gtattcatca gatattgacg tacttctttt tcattcaact gttggacatt gatgataatt
     4801 gctgtttttt tcaattgttt cgttactttt ttccgttcat ccaacttttc atatggcgca
     4861 aagaatacta atacagtcga ttctaatggt tctttcaaat aagccgtcaa taaatcagta
     4921 ttttgttcga ttccattgtt tactttttca cctgttaaaa aataaggatt ctctacgaat
     4981 actaaccgtt gatctccgaa aaacggcaac gtctctgctt cagctactac ctcatctaaa
     5041 cttgcatttt ccatgtcaaa acttaaaaaa tttaagtcat cttcgccctc taattggatt
     5101 tttttgacaa tttctgtccg aacttgatct tggagaaact tttctgttcc taaaatcaag
     5161 taacaagatg caagtttttc tttacgtatt ttttgtaact ctgcttgtat actcaaatta
     5221 ctcaccactt tcaaaaatta tcctaccatg agcagatgag aattactagt ctatcattgt
     5281 ttccactagc ccatgaggat gaaaaactgg atgccagcga taggtgatca ttccttgttg
     5341 atccgttcgc catgttttta tctgattttc ttctagtgtc tccaatactt cgctgtgcgg
     5401 gtgaccataa cgattattta cgccgcagga tataatccca tgttttgctt caatatggga
     5461 aagaaaatct gtgttacttg acgtacggct accgtgatga cctaatttga ctacatcggc
     5521 tttcagatta ggataaatca aaagcaattg tttttccatt gcttgattca aatctcctaa
     5581 aaataaaaag cgcctattgt tatattccat ccatagacca atcgaatctt catttcttcc
     5641 ctctcccttt tcaaaaggag tcaatacata aagatcataa taaccgctga tcctgtcccc
     5701 atgtttgatt tcaatgatct cagtaccatt tttttgagct tcagataaat gcctttgtaa
     5761 tggctcattt cgtccagcgc cccaaccgat ataaattttt ttgatagtaa aatgctgaga
     5821 taacgtgggt aattctccca catgatccgt atcgtcgtgt gtcaagatga gtttatcgat
     5881 tacacttatc ccctgacctt ttagatatgg aacgatattt ctttcagcag gaggacgtat
     5941 cataccttgc gcccactttt ctctttcaaa gtgtaactga cctccagtat cgatcaaaat
     6001 cgtttctttt cgaaaaggac tttgcaaaaa gatactgtct ccttgaccga catcgataaa
     6061 agcgatcgtt gaagaaaagc gtaaaaatgg caaagtcaaa aaaataaaag aaaaaagacc
     6121 aacataaaac aagtgtttca caggttgttc tttcaaccta tccatacaaa tcagtaatcc
     6181 gaaaatatac atcccagata gcacaagggg caaacgtcct ataatgagag acgaccctga
     6241 agaaaaatcg attattcgct caaataagat taatccctct tctatcagca aaagtaaagt
     6301 ttccggcagc cagcgattga agagtgtgag aaataacaca ccaggaagaa aaagaaactt
     6361 gaataccggt gcaatcagta atgtaaaaag actacctaaa ataggccact cataaaaatg
     6421 ccatacaatc aacggcatcg tgatcatagt aaacaggacg tgagaggccg tttttttcaa
     6481 ccaatctcca tcaaaagaaa tcaggataaa caaaaaactc atgaaaaaac taagctgtcc
     6541 acccgcatga aaaagaaatc gaggatcaaa tagtaaaaga agccaaaaga caactgcaaa
     6601 taagtccatg gtggaataat gcttattcag caatttgact agcaatcgca ataaaaacag
     6661 caaactagca cgcaaaacag aaatagaact tcctgcaata gaaacataac agatagttag
     6721 tagactcaac ggaataatac tgacgtgtag tggccatccg atacgtcgaa ataaataata
     6781 cagccaccct aaataaaatt ggatgtgtag acccgataga ctgaacagat gcagcaaacc
     6841 actctcccta aaaatttctt ctggttctct gaatcctgca tctttatagc cccataataa
     6901 agaattaata tatacacttg ttttttgcgg aaattcagat tggacaaagg agataaaaga
     6961 tcctcttttt ctctgaagcc agtttcgcca gtgattttgc atggtgatct gttcaatctt
     7021 ttgaatttgg aaccgtccag aaattctatg gttcctgaag taagcttctt ggtcaaaatt
     7081 ataaagattc cgcttttttg ctggagagat gaactcgcct tcagcagtca attctattcc
     7141 ctttcctcgg aagttaagcc atgttttcaa ttctgcttct gtgtttactt gataagaaag
     7201 ctggagcttt tgtttattct ctgataacct tccttccatt gtcaaaaaat taccatttgc
     7261 acgaatcgta tctgtaaata cagtaatttg cgcagatgtt gttttttcct ctaaaaatga
     7321 atactcttgt agcctataag aaatggatac cgttatgcta cacaaaatac acaagtacgc
     7381 gataaaacgt ccttctttta gcgaaaacca taaaaaaatt ccggcaagaa gcaaacgata
     7441 accccaattg tcagtgtaat acaacaaaca gataatcatc gacgtaattg ccggaaaaat
     7501 caaccgattt cgaacatgcc tttctaaaac gctagacatg ttttcctgtt aattcatttt
     7561 gaagttttga aaaatgctgt gagtctaaag caacttggtg tacagataca cctaattgtt
     7621 cgatcaacgc ttgggcataa ggatcatttc gatagtcatg gagataattg atccctttca
     7681 caccagcttg caacaaagcc tttgtacagg ccaaacatgg aaaatgtgtg acatagattt
     7741 ctgaaccttc cgtcgggata ccaagttttg cacactgtag gagtgcattc atctctgcat
     7801 ggatcgttcg caagcagtgt ccatccacga cgtaacaccc ttcatctatg cagtggacat
     7861 ctccactgac tgctccatta tagccgccag caatgatcct tttttctcga acgagtgtcg
     7921 ctcctacttc taaacgtgta cacgtactac gtaaagataa cagtactgcc tgagccataa
     7981 agtactggtc ccaaggaatg cgttcattat gtgacttatc agtaactttt tctttattcg
     8041 tatcgcatgc ttttgaagat ttttctaaca tgttttatat tcctccgttt tttcaagttg
     8101 attaaaaagt actcattgct taaacaaaat attctttaaa tataacgaat catcctgtct
     8161 gacaaaagat cctacccttc actgccgaaa tgaagtccgt catccattag cccattataa
     8221 aaattcttcg aatccatttg ttcaagaaat tccgtataaa aatcgatact tcacaatcat
     8281 ggcgttttgc tacttctgtg agaacagaga tatattcttt gatcaacttg ttattccgat
     8341 atttttgttt tgtctcatcg actgctggtg gcgtgataaa gaaagcatga tcagaactat
     8401 agtctttttt cagctgaact atcatccact gcagattttt tggtacgttt ctttagaaag
     8461 ttgtttatgc tctgctagat cattcgtccc aatgagaata aatactttat ctgctgtata
     8521 gctaagtaca tctttggcaa agcgttctcg tagagcttga ctgttattgc cgctgacagc
     8581 cgtattcata atatgaatcc cagatatttt attttggaga tagacattca gcattggttt
     8641 ttttaatgtt tctttgcgaa caatcaagct atttcctatc caaaaatact acctcctcgt
     8701 cctgccaagc tcttagaatt ggattattct tagtatccat tagcgtccat ttattgctcg
     8761 tgccagtcga ttttgaatca ttctatttaa ttgtaacgaa gttttttagt ttttcaactg
     8821 tcttttcacc gataccagaa atctcctgca ggtcttccac tttctggaag gatccgtttt
     8881 cttctctaaa tcgaatgatt tcttgcgctt tgactgatcc gatcccagga atctgctgaa
     8941 gttctgatgg agtggctgta ttgatattta ttttgggagg ctcggaatct gctgtaggct
     9001 gttttccttc cgaggatgcc aactgatcaa gatgttctgg cagctcttca cctacagcag
     9061 ggatatatag gacttgctga tcttctatct tcgcagaaaa gttgattcga tcgctatccg
     9121 cttcttctaa taatccacct gcacgcttta gtacatcata gacccgttct tctgaagaaa
     9181 aggaatagat accaggctcc ttcacacttc ctttgatatc ggtgtacatg acctttggac
     9241 gagcatcctc ttctacatcg tttccttcag ctgatggatc ttctccactg gaactaaacg
     9301 aagaaagtat ttcttttgca gcagtctctt ctgtttctct ataaaatagc aaatttgata
     9361 ataaaaaacc acttgcgata caaaagataa gaact
//
LOCUS       DAYYCY010000101         9298 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000101 DAYYCY010000000
VERSION     DAYYCY010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9298
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.101"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..334)
                     /locus_tag="ACHAZB_001951"
     CDS             complement(<1..334)
                     /locus_tag="ACHAZB_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HFM8614591.1"
                     /translation="MRNKKVIVRILIVSFLLFGCFKLIKTNEESNGKLINSNLEEVLE
                     LQKTNQSFFVYLARNDCKKCQEVESFLERYDKKFSRNVYRIETRKEPRQEELQTFLQR
                     YHVKSVPSF"
     gene            complement(324..2498)
                     /locus_tag="ACHAZB_001952"
     CDS             complement(324..2498)
                     /locus_tag="ACHAZB_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="HFM8614592.1"
                     /translation="MMNAPKRPTHFEQMFSVDRKRKQEKETPKSRFQQLYFIVTLLGV
                     CLYPFFIVGSIPLAIVFMIDKKETRAHIYDFEYESFLKRRSSLFFIFALGFTLLNLFC
                     FIFVLPRGYLSCYLLFPLNLFSHALQFNGQTIFALLLGGTGAGSCLIAYASFIEKRKV
                     VSKKERQTALLNSKEYKERRKNKFQESQKYTAEYQQAYQVAIVIKDVALRNKQLASLA
                     KVILLGTDEYGLPYVMNFSELNQHVIIPATTGSGKTTVIQLLAEHACKFRIPVILVDG
                     KGAGETLQALKEVAEAYNRPIEVFVDGGDLRYNPVKNGNDIAIRDKLTNLAETESVYY
                     STAAKALLQVTIQLLDEFQKSHKIERNLVWLQHYLLPRNVLHLFADRIQLKNPDLFKE
                     KVVVEKKKTTKKKTKKNEAMDKTERDFADVEQLSEERIVEHDRKTVDLETYYLLLKEN
                     ISLMTEIEQTLFKRLFVRYEHKKNPFYLYATSENLQTNINMLLDSELGELFDTSGGKR
                     ELDIQEVVKEGKIVYISLNGLIYSEFIRTLAQMLVGDINFFASEVYKQAGETANRSKV
                     LVIFDEPASYLNDNLIDLVNKGRGAGVHAAYAPQTLADIDRIDPVLRKQLIGNVNTFF
                     IGKTNDPTEIDYWADLMGTEEDIELTEMTTQEAGYSDMGKVDWTGERGTKRDVDRFKF
                     NPNRIRALRTGEFVVYRTAEDVQEPPRVVYIRKPNLEKQYAK"
     gene            complement(2514..4856)
                     /locus_tag="ACHAZB_001953"
     CDS             complement(2514..4856)
                     /locus_tag="ACHAZB_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFM8614593.1"
                     /translation="MRKKSLKWWTLLLLGGLFFCLQGATVSADQMNDGAGVTETDRSE
                     GENANDKVEVKEVTTDVDQLYKNYKKNSFELMTNDYESMNLKKKLSYNVAGVMKNFVW
                     SGVKMLGEFNASMVEFLFELDIISAIREPIQNLTQKIADNMLSIAGTIGISFSAIMMA
                     IKYAGEARMKQALRVFLMTILIFTGLSVLKDSGTNKNLFDVLFSLDSTVETAFVKVNP
                     VLTPEEETNYETKETEEVTADKKVETAGKLMAARVFYSNVYEPYLLLNYGTTNVDTIR
                     EKKVKYAGKEYDRINLLLDNDLGTKIGEKIHDKVTEYEADTLKNKNIQYYNSWKNTAF
                     ALFYFVVNLIQTVVYFVLSMFRLIIGTMQLLLVPMLPFLLFLGLFLVTMNVFGNYAKV
                     FGILTFLKGMTGFACILFSSFLSYGFQLSSTQDDPWEKILLIIVYLFAPLGIYLFRGF
                     LGSLFTGRMGVRGALAFAGNPFGAEAKLRREAKQRLKERKEARKAQKQKEKENARKRQ
                     ERQRKRGGGSDVRMPQTAKQATRSAFRRESAQQPRHKAPNRMERMAQKARDLHDQAKQ
                     AEEQSQERMQRTAMEKQARKTADLLQRELQGNPSLRERSRKGASRSPYRNQGSAMVVS
                     LANPKQKHKSYGQKHTPRTELNDRKRRQGQTSRRRKEALTPVTKTSSMKTRTVAGQKH
                     AVQKPLRKGNVPRATGSTTPKSVRRPEGVREHMSAVNRVTAQTKAPSTVSKAYSTMKQ
                     TKSPKQAPKTRSEFRATQAKMKRKREKQPIYRREMKQRKR"
     gene            complement(4878..7421)
                     /locus_tag="ACHAZB_001954"
     CDS             complement(4878..7421)
                     /locus_tag="ACHAZB_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8614594.1"
                     /translation="MAFLFLLVVFIIGIWQFKKKKTKLPRLEFPIDAIIRNFVLTKND
                     DVWVGYQLSSQVFPLNDIDFFKNYLQDGEGILGHDEYDYHFINIPRQFNLDTHIEETI
                     RDLVKGECADLGKVYFEQAGKILKDEVQLNEYRTYLFVRFTTEMKVSDPIEYFQLLKH
                     VFKKFIYRITGQRVPISSVLPTYETLEKELYTELSNYKPIERLSAQEIGQIYYYFFHR
                     SEEKLPERQLTPMEICEGVISNEAGYIAIEQLNQTHYSTFLSQTDAPLAMYGSSFIQN
                     LQDSLSFPIDTHIRLRFEHSKSDERKINKMAGRIYEQEKEYETVDGILDSDDVLNFGT
                     ERLMDVKDKLKRKEKRLCYSTVTLVVSASSKEELEERVKDIEFAIESTDFKFYRSVAD
                     QLTLFNQCLIGSTQRFRSYEQVATTGFVADYGLDLKKEVGNKKGFPLGRIVIAKKFKS
                     AKEALALSSKLVWYYPNLTKKSIEGAEHTNGNTLIIGPPGKGKSVLVKYIFLWLMFLG
                     QKILYIDPKNETQKFFLKALEKFGHIPEFVAMYERINFISLSSDESCRGMLDPLLFLP
                     KEEAIQTAKNVLGMLGEVDTNRETASHKKTVIQDAIDVVYTMPGKHSLSQVIEEIRKS
                     DKELAKLILGHSVGLGKILIGNEHSTPIRFDSQINVLGTQGIRIPTQKEIDSGRLNSE
                     QLAGMAIMEVIMKFTYIFSTNKEEDAAIIYDEAKGYEDTAQGSYLIDDSLRKGRANNT
                     DIYLVVQAFKDYDQEDKKELISYKFAFRPKQKEAREKLLSFFDMEVNSANLEMMEQLQ
                     TGTCLFQDHLGRNQPIAIDVLFDSWLLAVSSTENQDEKIQAMLENEKQR"
     gene            complement(7431..7829)
                     /locus_tag="ACHAZB_001955"
     CDS             complement(7431..7829)
                     /locus_tag="ACHAZB_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpE family conjugal transfer membrane protein"
                     /protein_id="HFM8614595.1"
                     /translation="MEYNYTKEFKHPHKIYGIKGQKIPWISNGIRMEQLVIGGVFLFI
                     LLILFLIAYVNQISFLQTLFRKSWLIMIFGIGVLVWTLFSMKWDNKHFFEYLIGRTSY
                     YQKRKQRFEHEMYVPFFHEKVTYSLHKWKE"
     gene            complement(7910..8194)
                     /locus_tag="ACHAZB_001956"
     CDS             complement(7910..8194)
                     /locus_tag="ACHAZB_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8614596.1"
                     /translation="MKLFDLKSLFDDVSGQVIWLLAIVAIVLMVGAYATQGFARAAFA
                     IIGVFILIMLVLLLSNAVDIGEWLKNIIFIKDSEENVGVTQMIVPWIQLS"
     gene            complement(8218..9216)
                     /locus_tag="ACHAZB_001957"
     CDS             complement(8218..9216)
                     /locus_tag="ACHAZB_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM8614597.1"
                     /translation="MSNAKFNPQAFQTKPPKQQKEKKQKKRFLFQKKKTEPSDRMIQF
                     SQKSAMSVFSGAFFCLFLLMGVVLLLNFGRVDTLTRLAVKKQVNPETLLTEVNQTLSS
                     TEQLKYEGQHFIERLFTYSPEKRNEWEASVNHYLAQGLEASDLGFVTTATNRVAKDVR
                     FIKLEEIDLTQAMYRLFYEVTFTEGEHWKSSQLSFPVSYQNNVLKVLDGVQFLNLSES
                     KNTNSVNYQENRFYSSGQAVTESEEIKLEAFVKRFFELYVKNDEKLALISNEKGLEGG
                     ELINVQLDHAVKNKEGNYFLQGKYRFYFEKGNEFQSNFSMEVKTSGDSYFVTTLNE"
BASE COUNT         2644 a         1994 c         1457 g         3203 t
ORIGIN      
        1 gaaaacttgg gacactttta acatggtaac gttgtaaaaa agtttgaagt tcttcttgtc
       61 ttggttcttt tcttgtttca attcgatata cattcctaga gaattttttg tcgtatcttt
      121 ccaagaaaga ttcaacttct tggcattttt tacaatcatt tcttgctaga tacacaaaaa
      181 atgattgatt cgttttttga agttctagta cttcttctaa attactattg attagttttc
      241 catttgattc ttcgttagtt tttatcagtt taaaacaacc aaataagagg aaactaacaa
      301 taagtatcct aacaataact ttcttatttc gcatattgtt tctccaaatt tggtttccga
      361 atatagacga ctcgcggtgg ttcctggacg tcttcagctg ttcgatacac cacaaattcg
      421 cctgttctca atgctcgaat gcgattcgga ttgaatttaa agcggtctac gtctcgtttc
      481 gtgcctcttt ctcctgtcca atcaaccttt cccatatcag aataacctgc ttcttgtgtc
      541 gtcatttccg ttaattcaat gtcttcttct gtccccatta aatctgccca ataatcaatt
      601 tctgtcgggt catttgtctt cccaataaag aacgtattca cattcccaat caactgtttt
      661 cgaaggacag gatcaattct atcaatgtcc gcaagcgttt gaggggcata cgccgcatgc
      721 accccagcgc ctcgtccctt attgactaag tctataaggt tatcatttag ataagaagct
      781 ggttcgtcaa atattactag gactttcgag cgattggctg tttctcctgc ttgtttatac
      841 acttctgacg caaagaaatt aatatctccc acaagcattt gagccaatgt ccgaataaac
      901 tcactataaa tcaatccgtt taacgagata taaacaatct ttccttcttt aacaacttct
      961 tgaatatcta attctctttt tcctccagaa gtatcaaaga gttcaccaag ctcactatcc
     1021 aataacatat tgatattggt ctgtaaattc tcagaagtcg catacaaata aaacgggttc
     1081 ttcttatgtt cgtaacgaac aaacaaccgt ttaaacaacg tttgctcgat ttctgtcatg
     1141 agagaaatgt tttcttttaa taatagataa tacgtttcta agtcaacggt tttacgatca
     1201 tgttcaacga ttcgctcttc acttaactgt tccacatcgg caaaatcacg ttctgttttg
     1261 tccatcgctt cattcttttt cgtttttttc ttcgtggttt tcttcttttc tacgaccact
     1321 ttttctttaa ataaatctgg atttttcaac tgaatacgat ccgcaaacaa atgtaagacg
     1381 tttcgtggca ataaataatg ttgtaaccaa actagatttc tttcaatttt atggctcttt
     1441 tggaactcgt ctaaaagctg aatcgttacc tgtaataacg ctttggctgc tgtcgaataa
     1501 tacacgcttt ctgtttctgc tagattggtt aatttatctc gaatggcaat atcattgcca
     1561 ttttttacag gattataacg caaatcgcca ccgtccacaa agacttcaat cgggcgattg
     1621 tatgcctctg ccacttcttt taacgcttgt aaagtctctc ctgctccttt cccatcgact
     1681 aagataacgg ggatacgaaa cttacacgca tgttctgcta gtaattggat caccgtcgtt
     1741 tttcctgaac ctgtagtcgc tggaataatc acatgttggt tcaattcact aaagttcatc
     1801 acataaggga gcccatactc atctgttcct aatagaatca ctttggctaa tgaagccagt
     1861 tgtttattcc gcaaagcaac gtcttttatg acaattgcta cctggtacgc ttgctgatac
     1921 tctgctgtat atttttggga ctcctgaaat ttattcttcc gacgttcctt gtattcttta
     1981 ctatttagca aagctgtttg ccgttctttc tttgaaacaa cttttctttt ttcaataaaa
     2041 gacgcatacg caatcaaaca acttcctgca cctgtcccac ctaaaaggag ggcaaaaatc
     2101 gtttgtccat taaactgcaa cgcatgagaa aacaagttta atggaaataa taagtaacac
     2161 gacaaatacc ctctaggaag aacaaaaata aaacaaaata gatttaatag cgtaaaacct
     2221 aacgcaaaaa taaaaaaaag agagcttctt cgttttaaga agctctcata ctcaaagtca
     2281 taaatatgtg cacgtgtctc ttttttatct atcataaaga caatcgctaa aggaatactg
     2341 cctacaataa aaaatggata tagacaaaca cctaataaag tgacgataaa ataaagttgt
     2401 tgaaaccgac tttttggcgt ttctttttct tgttttcttt tgcgatcgac agaaaacatt
     2461 tgctcaaaat gtgtcggtcg ttttggtgca ttcatcatac tatcgatttc ctcctatcgt
     2521 tttcgttgtt tcatttctct tcgataaatc ggttgttttt ctcgttttcg tttcattttt
     2581 gcttgagttg ctcgaaactc actccgtgtc ttcggtgctt gttttggtga ttttgtttgt
     2641 ttcatggttg agtatgcttt agatacggtt gaaggcgctt tggtttgtgc tgttactcga
     2701 ttgactgctg acatatgttc ccgaaccccc tctggacgtc gaacagattt cggtgtggta
     2761 gaacctgttg ctcgtggtac attccctttt cttagtggtt tttgtaccgc atgtttttgt
     2821 cctgcaactg tccgggtttt catcgaactt gtttttgtta cgggagtaag tgcctccttc
     2881 cgacgacgac ttgtttgtcc ctgacgacgt tttcgatcgt tcaattctgt tctaggcgta
     2941 tgcttctggc cataagactt gtgtttttgc tttggattcg ctaatgacac caccattgca
     3001 gaaccctgat tgcgatacgg actacgagac gcaccttttc tacttctttc tcttagacta
     3061 ggattgcctt gcaattctcg ttgtaataaa tcggctgttt ttcttgcctg tttctccatt
     3121 gctgttcgtt gcatacgttc ttgactttgc tcttctgctt gtttcgcttg atcatgcaag
     3181 tctcttgctt tttgtgccat acgttccata cgatttggtg ctttatgacg aggttgttgt
     3241 gcggattcac gccgaaaagc acttcgtgtg gcttgtttcg ccgtttgtgg catacgaacg
     3301 tctgagccac ccccacgttt tcgttgacgt tcttgtctct tccgtgcgtt ttctttttct
     3361 ttttgtttct gtgcttttct cgcttctttg cgttctttca accgttgttt ggcttccctt
     3421 ctcaattttg cttctgcccc aaatggattt cccgcaaaag ctaacgctcc tcgaacaccc
     3481 attcttccag taaatagact gcctaaaaag cctcggaaca aataaattcc caacggcgca
     3541 aacaaataaa caatgatcaa gagaattttt tcccatggat cgtcttgtgt actacttaat
     3601 tggaacccgt aactaagaaa agaagaaaaa aggatacatg caaaacctgt cattcctttt
     3661 aaaaacgtca aaataccaaa aactttggca tagttaccaa acacattcat tgtgacaaga
     3721 aacaatccta aaaaaagtaa gaacggcaac atcggtacta acaatagttg catagttcca
     3781 ataatcaatc gaaacatact taagacaaaa taaacaacgg tttgaatcag attcacgaca
     3841 aaataaaaca aggcaaatgc tgtatttttc caactattgt aatattgtat gttcttgttt
     3901 ttcagtgtat ctgcttcata ctctgtgact ttgtcatgaa ttttctcccc tatttttgta
     3961 cctaaatcat tgtccaataa aagattgatt cgatcatatt cttttccagc atatttgact
     4021 ttcttctcac gaattgtgtc tacattggtt gtgccataat ttaacaacag atatggttca
     4081 taaacattac tataaaagac ccgagccgcc atcaatttgc ctgccgtttc cactttttta
     4141 tccgctgtta cctcttctgt ttcttttgtt tcataatttg tttcttcttc aggtgttaac
     4201 actggattga ccttcacgaa cgccgtttcc actgtagaat ctaaagaaaa aagaacatca
     4261 aaaagattct tatttgtccc agaatctttt aatacgctta acccagtaaa aataaggatc
     4321 gtcataagaa agacacggag tgcttgtttc attcgagcct ctccagcata tttgattgcc
     4381 atcataattg ccgaaaaact gattccaata gttcctgcaa tacttaacat attgtccgca
     4441 attttttgtg ttaagttttg aatgggttct cgaatggcgg aaataatatc taattcaaag
     4501 agaaattcaa ccattgaagc attgaattct cctaacattt ttacgccact ccaaacaaaa
     4561 tttttcatca ctcctgcaac attgtacgat agcttttttt taaggttcat tgactcatag
     4621 tcattggtca tcaattcaaa cgaattcttt ttgtaatttt tatacaattg atccacatca
     4681 gtcgttactt cttttacttc tactttatcg ttggcattct ctccttcaga acgatcggtt
     4741 tcagtgacac ccgcaccatc attcatttga tcggcagaaa cagtagcccc ttgtagacag
     4801 aaaaaaagac cccctaataa aaggagcgtc caccatttca gagatttttt tcgcaaaaaa
     4861 accacctctt ttttgtttta tcgttgtttt tcattttcta acattgcttg aattttttca
     4921 tcttgatttt cagtagaaga aacagccaat aaccaactat caaacaacac atcaatggca
     4981 atcggttgat ttcttcccaa atgatcctgg aataaacagg ttcctgtttg taattgttcc
     5041 atcatttcta aattggctga attcacttcc atatcaaaga aggacaatag tttctctcgt
     5101 gcttcttttt gtttcggacg aaacgcaaat ttgtaagaaa ttaactcttt tttatcttct
     5161 tgatcgtaat ctttaaacgc ttgcacaaca aggtaaatat ccgtattatt cgcacgtcct
     5221 tttcgcaagc tatcatcaat caaatagctt ccttgtgcgg tatcttcata cccttttgcc
     5281 tcgtcataaa taatcgcagc gtcttcttct ttgtttgtag aaaagatata cgtaaacttc
     5341 ataatgactt ccataattgc cattcctgca agttgttcgc tatttaatcg acccgaatca
     5401 atctcttttt gtgtcggaat gcgtattcct tgtgtcccta acacgttgat ttgtgaatca
     5461 aaacgtatcg gcgtactgtg ctcattccca atgagaatct ttcctaaacc aacgctatgc
     5521 cctaaaatca atttagctag ttctttgtca ctttttcgaa tttcttcgat aacttgactt
     5581 aacgaatgct tacctggcat ggtgtaaact acatcgatgg cgtcttggat caccgttttc
     5641 ttgtggctcg ctgtttctcg attggtatct acttctccta acattcctaa cacattttta
     5701 gcggtttgaa ttgcctcttc ttttggtaaa aacaatagag gatcaagcat ccctcgacag
     5761 ctttcatctg aagataacga aataaaatta attcgttcat acatagccac aaactctggt
     5821 atgtgtccga atttttcaag tgcctttaaa aagaactttt gggtttcatt tttcggatca
     5881 atataaagaa ttttttgacc taaaaacatc agccataaaa aaatatattt tacaaggacc
     5941 gattttcctt tacctggtgg accaataatc aacgtatttc cattggtatg ttccgcgcct
     6001 tcaatacttt tctttgttaa attgggataa taccatacta gtttactaga tagcgctaac
     6061 gcttctttcg cagatttaaa cttttttgca ataacaatcc gtcctaaagg aaatcctttc
     6121 ttatttccta cttctttctt taagtccaag ccataatctg ccacaaaacc tgtcgtagcg
     6181 acctgctcgt aactacgaaa acgttgcgta cttccaatta agcattgatt aaacaacgtt
     6241 agctgatccg ctaccgatcg ataaaatttg aaatctgtag actcaatcgc aaattcaata
     6301 tctttgactc gttcttccaa ttcttctttt gaagaagcag atacaaccag cgtgacggta
     6361 gaataacaca atcgtttttc tttgcgcttc agcttatctt ttacatccat taaccgttct
     6421 gtaccaaaat ttaatacatc gtctgaatct aaaatgccgt caacggtctc atattctttt
     6481 tcttgttcat aaatccgtcc agccatttta ttaatttttc gttcatcaga ttttgaatgc
     6541 tcaaatcgaa gtcgaatatg cgtatcaatt ggaaaagata agctatcctg aagattttga
     6601 ataaagctac tcccatacat ggctaaagga gcgtccgttt gacttaaaaa cgtcgaataa
     6661 tgcgtttgat tcaactgttc aatcgcaatg tatcctgctt cattcgaaat gacgccctcg
     6721 caaatttcca ttggcgtaag ctgtctttca ggcaattttt cttctgatcg atggaaaaaa
     6781 taataataaa tttgtccaat ctcttgggca gataagcgct ctatcggctt ataattggac
     6841 aattccgtat agagctcctt ttctaatgtt tcatacgttg gtaacacact acttatagga
     6901 actcgttgtc ccgtaatacg atagataaac tttttaaaaa catgcttaag caattgaaaa
     6961 tattcgatcg gatcagacac tttcatttct gtagtgaaac gcacaaacag atacgttcga
     7021 tactcgttta attgcacttc gtcttttaaa atctttcctg cctgttcaaa gtacactttc
     7081 ccaagatctg cgcattctcc ctttaccaaa tctcgaatgg tttcttcaat gtgcgtatct
     7141 aggttaaatt gacgtggaat attgataaaa tgatagtcat attcatcatg tccaagtatg
     7201 ccttcgccat cttgaagata gttcttgaaa aaatcaatgt catttaaagg aaatacttgg
     7261 gaagaaagtt gatagcctac ccacacatca tcatttttgg tcaaaacaaa atttcggata
     7321 atggcgtcta tcggaaattc taatcgagga agcttcgttt ttttcttttt gaactgccaa
     7381 atcccgataa taaagacgac caaaagaaac aaaaacgcca tttactcgac tcactccttc
     7441 catttgtgta gggaatacgt cactttttca tggaaaaacg gcacatacat ttcatgttca
     7501 aagcgttgtt tccgtttctg ataataggac gttcgcccaa ttaaatactc gaaaaagtgt
     7561 ttattgtccc atttcattga aaacaatgtc caaacgagaa caccaatccc aaaaatcata
     7621 atcaaccagg actttcgaaa cagtgtctgc aagaaactaa tttgattcac atacgcaatc
     7681 aaaaatagga tcaaaagaat gaagagaaat accccaccta tgactaattg ttccatgcga
     7741 atgccattag aaatccaggg aattttttgt cctttaatcc cgtaaatttt gtgagggtgc
     7801 ttaaactcct ttgtatagtt gtattccatg ttcactctac ctccttttac aaaaaaagaa
     7861 gcgaccagaa gttctaatct tctgatcgct tttgcagttt gtctttttct tatgataatt
     7921 gtatccaggg cacaatcatt tgcgttaccc ccacgttctc ttcactatcc ttaataaaga
     7981 taatgttctt tagccattca ccaatatcaa cggcattcga taacaataag acgagcatta
     8041 tcaaaataaa gacgccaata atggcaaacg ctgcccgcgc aaagccttgg gtcgcataag
     8101 caccaaccat tagaacaatc gcaacgattg ccaatagcca aatgacttgt ccagatacat
     8161 catcaaataa cgatttaaga tcaaaaagtt tcatttccat tctcctctaa gttcatttta
     8221 ttcatttaac gtagtaacaa aataactatc gccacttgtt tttacttcca ttgaaaagtt
     8281 tgattggaac tcgtttcctt tctcaaaata gaaacgatac ttcccttgca aaaagtagtt
     8341 tccttcttta ttttttactg catgatccag ttgtacattg atcaattcgc cgccttctaa
     8401 ccctttttcg ttagaaatca atgccaattt ttcatcattt ttgacatata actcaaaaaa
     8461 ccgtttcaca aatgcttcca gcttgatttc ttcgctttca gtgaccgctt gcccagaaga
     8521 ataaaatcga ttttcctgat agttcacaga attggtattc ttactctcag aaagattcaa
     8581 aaattgaacg ccatcaagta cttttaaaac attgttttga taactgactg gaaaagaaag
     8641 ttgactgctt ttccaatgtt ctccttctgt aaaagtaacc tcataaaata ggcgatacat
     8701 tgcttgtgtc aaatcgattt cttctagttt aataaagcga acatctttcg ctactcgatt
     8761 ggtagccgtc gttacaaagc ctagatcaga cgcttccaat ccttgtgcta aataatgatt
     8821 gacggaagcc tcccattcgt ttcgtttttc tggcgaataa gtaaataacc tttcaataaa
     8881 atgttgtccc tcgtacttca attgttccgt agaagacaag gtttggttga cttcagttaa
     8941 caatgtttct ggattgactt gttttttcac tgctaaacgc gttaatgtat ccactcgtcc
     9001 aaagtttagg aggagcacga cacccattaa cagaaacaaa caaaaaaaag cgcctgaaaa
     9061 tacactcatg gcactttttt gcgaaaattg gatcattcga tctgacggtt ctgttttctt
     9121 tttttggaac aaaaaacgtt tcttttgttt cttttccttc tgttgtttcg gtggcttagt
     9181 ttggaacgct tgtggattaa attttgcatt actcatgaga aatgccatac cgttctaaca
     9241 ataatttcgg tattttgtcc acaatcacta cttcgtggac aaccacactt gccccctc
//
LOCUS       DAYYCY010000102         9139 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000102 DAYYCY010000000
VERSION     DAYYCY010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9139
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.102"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            20..2797
                     /locus_tag="ACHAZB_001958"
     CDS             20..2797
                     /locus_tag="ACHAZB_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM8614598.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            2878..3297
                     /locus_tag="ACHAZB_001959"
     CDS             2878..3297
                     /locus_tag="ACHAZB_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8614599.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            3309..3779
                     /locus_tag="ACHAZB_001960"
     CDS             3309..3779
                     /locus_tag="ACHAZB_001960"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8614600.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            3796..4575
                     /locus_tag="ACHAZB_001961"
     CDS             3796..4575
                     /locus_tag="ACHAZB_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM8614601.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            4562..5383
                     /locus_tag="ACHAZB_001962"
     CDS             4562..5383
                     /locus_tag="ACHAZB_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8614602.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            5400..6449
                     /locus_tag="ACHAZB_001963"
     CDS             5400..6449
                     /locus_tag="ACHAZB_001963"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM8614603.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            6462..7466
                     /locus_tag="ACHAZB_001964"
     CDS             6462..7466
                     /locus_tag="ACHAZB_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM8614604.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            8099..9118
                     /locus_tag="ACHAZB_001965"
     CDS             8099..9118
                     /locus_tag="ACHAZB_001965"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFM8614605.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT         3043 a         1533 c         1937 g         2626 t
ORIGIN      
        1 tttctatgat ggaggagggg tgaataagtt gttgaagaag gaaattttat gttacttaga
       61 gaaccagaca gcctttttcg atttagaaca tatgaacgag gtctttacag cacaaaggtt
      121 agctgatcat tttggggtaa aaagaaacac agtcagtcat tatttgaacc agttaaatga
      181 tgagcaacta ctagtcaaaa tcaattctcg accagtgatt tatttccaca aagaagcttt
      241 tgaaaaacaa ttttttaagt tacgcacgaa tttttatttt tctatcgatg atctcaaaga
      301 ggaacagcct tttttcgcgc aaccaaaaga cttattctct tttatgatcg gatacgacgc
      361 cagtttgaaa gaaagtatcg aacaaatcaa aacagctctt tactatccag atggcggatt
      421 accacttttg attacaggcg aaagtggcac aggtaaaagt tatttagtta atttggtcta
      481 tcagtattgt ctgcttcatg atctgctaga agattctgca ccattcatca cttttaattg
      541 tgcacaatat gcagataatc ctgaactttt gactagcaat ttgtttggac atgttaaggg
      601 agcgtacact ggtgcagaag aaaataaaaa aggggctttt gaagcagcag atggtgggat
      661 cttgttttta gacgaggtac atcgcttaac accagaaggt caggaaaaac tgttcaccta
      721 tcttgatcaa ggtgtgattt atcgtatggg agaacctaat ttgtcacgcc ggatcaaaac
      781 acgtctcttt tttgctacga ctgaagagat tactagtcat ttcttaacaa ctttcattcg
      841 aagaatacct atccgaatcg aactacctgc acttaatcag cgtagtcgta atgaacggct
      901 agaactgatt tactcttttt ttctcgaaga acaaagaaaa atggggagcc ctttgtcagt
      961 cagtggacca gctttgtcac ttttaacaag tcagaaatta tccggaaaca taggagagct
     1021 gaaaaatatc gtcaaagttt cagcagcaaa agcatatgct gaacagcgtg aacagcctga
     1081 aatccatgtc acgatccatc atttgcaaaa agaactactt gcgcagccta ttactaaaaa
     1141 tacagcacag caaggaatct atattacgca agaccagacc ttagagcaat taatggagaa
     1201 gcaacagccc gaacagcagc gaatcgttaa aagttttgag caaatcctaa ttgatttcaa
     1261 gaaaaataac tgtctgctat attcttgcga aggaaaatta aagcaagaag tgattcaatt
     1321 atttgatttt ttgctgtttg agaccagtag acaagaaaaa cacgagctgc tggtttattg
     1381 gacacaatct atccgagaga cctttcgaca aatggaaact gcctatcaaa tcaaattcga
     1441 tggaaacagt gtctatgcct tgagttacta tctctatcag cgaagcacta tcaaatggtt
     1501 accggaagaa catgatatta tccaactaat caaagaatta gaacaacaag tttctcagtc
     1561 ttatccatct agttatcact atgtccaacg aattctggaa ttaagtaaga tcaaattaga
     1621 tattgaagac acaagtatgg atcggatttt actgacgatc tatctaaaaa aagcgaaatg
     1681 gtcaaaagaa aaacgactgc caaaagcgat gattgctgct cacggttatg caactgctag
     1741 cagtatggca aatgtagtaa atcgtttatt acaagaggat ctatttgaat catttgatat
     1801 gccactagat gtcacaccac agcagattgc ttcagaaatc cttgattatt gcgaaaacag
     1861 cgatgtttca aatggattga tcatattagt agacatggga tctctgaaag aaatccatca
     1921 gttttttaaa aaacagttat ccgtaccgct tttgattttg aataatgtga cgacaccttt
     1981 agcaatcaca gtaggggaat gcctgcaaaa aaatgtatca ttagaagaga tagcagaaga
     2041 gactgttcga cagatccagc cagaatggcg actgctctat ccagaggaaa acaaacccaa
     2101 agcactcatt actacttgct tcactggaat cggtacggcg gtccacttaa gtgaactgct
     2161 agaaaaaagc ttacctacgg cttgtcagtt gaaaatcatc ccatatgaat atcagcaatt
     2221 aaaagataag aaaaacagtg acccattatt ttccatatac gaagtcgtag gaatgattgg
     2281 caccacagat cctgatatca ccaatatcga ctatctttca ttagaagacc tcatctcggg
     2341 ggaaaagatg tctgtactag ctgaatggtt ggacactact atgaatgctt cagagaagga
     2401 aatattcaat cagcgcatca ttcgtaattt ttcattggaa aaagtactgg attccgtgac
     2461 tattctagat acagagaagg tgatgggaga aatcgatcat tttatgagag aattagaggt
     2521 gcagttgaat cgaagcatca gcaatcccaa aaaattagcg ttgtatgtcc atgtgagctg
     2581 tctcattgaa cgattgatcc gacaaatgcc aatcgaaacc tatcaaggat tagagaaatg
     2641 gaagcagtgt caaaaagaag ggttatctgc tattaaatct gcgtttagtg tcattgaaga
     2701 gcattatagt gtcatgatcc ccaattctga gattgcctat atctatgata ttttgagtga
     2761 agatacagag tttctctcga tagaagaaga attttgaaca taagcaagtt gttgccacta
     2821 attttggcat gcaacttgct tttttagtat gtgtaagtga aaagaggaga tgaaaaagtg
     2881 gatagagaaa ttatcttagc ttcccatgga aaatttgctt cagggatatt agattcatta
     2941 gagctgatct atgggaaaaa tcattctatt actgtattcg attgctatac tgatgagaat
     3001 tttgatttag cggaaacagt aaggcagctt tttatgaggt acaaagataa agaaatcatt
     3061 gttctgacgg atctttttgg cggaagtgtc aacaacgaat tcttacaata tatcaatcgg
     3121 gatcatgtct atctcgttgc cggattgaat ttgccactat tgattgagct agtatctcgg
     3181 ttggacgctg aagtagagac aactgttttg atccagcagg tgctaactga ttcaaaaaaa
     3241 atgatccagt tttgtaacga gcggctcaat cagaaaatag aagaagacga attttaggag
     3301 gaagaaaaat gattgtattg acaagagtcg accatcggtt attacacgga caagtagctt
     3361 tttcatggac acaaacaata ggcgcagact gtattttgat tgctaatgat gatgtaccta
     3421 cgaatgagat acgaaagaca acgatcaaat tagccaagcc acaaggagtc aaactagtga
     3481 tcaaatcaat tgatgactcg attgccgcgt tgaaaagcgg ggtgacagat aaatataagt
     3541 tattcatcgt agtggaatcg atcgaggatg catataagct ggcagaagcg tatccagaaa
     3601 tcaaaaccat caacttagga gggatcaagg caaaacctgg tactcgcagc atcggaaaag
     3661 cggtaaatat tttaccagaa gaggaggaac gcttgaaacg attaattgac aaacaaatag
     3721 aagtagaaat ccgtcaagta ccagcagata aaaaagtgga tgtaagagaa gttttgtaag
     3781 aaaggaagaa cctcaatgat tgtacaagca gtcttattag gaattgttgc atttgtcgca
     3841 caatcagaat atgcactagg gacatcgttg ctttctcgtc cgattgtgac ggggctgttt
     3901 acaggaatcg tgttaggtga tgtaaaaact gggatcatta tgggcgcaac tttagagtta
     3961 gcatttatcg gatcgttctc agtaggtgct tcgattccac ctgatgttgt gaccggtggg
     4021 attttaggga cggcttttgc tattacagct ggagccggta cagaaaccgc gcttttacta
     4081 ggtttgccga ttgctacgtt gacattgata ttgaaaaata tttacttagg gcttttgatt
     4141 ccaattatga accataaagc agacaattat gcagaagaag gaaattataa aggagtcgag
     4201 catatgcact tgcttgccgg tatcggtctt tccttgatgc ttggtgttgt ggtaatggtt
     4261 tcttatatgg tgggaagcaa tacgatcgga aatctattaa atatgattcc tgacttcgtt
     4321 caaaaggggt tgtcagtagc tacagggttg attccagctc ttggttttgc gatgcttgct
     4381 cgtttgctga tcaacaaaca agttgcacca tattttttct taggttttgc tatagcttct
     4441 tacttagaga ttcctgtgac cggagtagct atttttggtg cgattttagc agttgtggta
     4501 gtcaatatta tgaatgtccg tcaactacca cttcaaacaa ctgcagggga ggtagaagaa
     4561 gatgaagaat tctgagatca aaaacagcaa aataacgaaa aaagagctaa atcacgtatt
     4621 ttggcgttct tttcaaatgg agttttcttg gaattatgaa cgtcagatga atatggctta
     4681 tgtttatgca atgattccga ttttgaaaaa gttatatcag tcgaaagaag aaatggcagc
     4741 agctttgaaa cggcatctag agttttttaa cacgacccca catatcgtta cactgatttt
     4801 aggaatcacg gctgcaatgg aggaagaaaa taaggatgat cctgaatttg atgtgactgc
     4861 gatcgatagt atcaaaacat ctttgatggg acctcttgca gggttaggag attctttctt
     4921 ttggggaact ttgcgattga ttgccacagg tgtgggtacc tctttagcca tgcaaggaaa
     4981 tattttaggc cctatcttgt tcattttgat tttcaatatt ccgcacattc tgtttcgcta
     5041 tctagcaaca ggttggggat atcgcttagg aacgggtttt ttgaaaaaga tccaggcaaa
     5101 cggtatgatg gaaagtttga cattaggcgc gtctattatt ggactgatgg tcgttggagc
     5161 aatgactgcc acgatgatca ccatcaatat tcctttaaaa atcggttcag gagaaaatgc
     5221 tgtgactgtt caaagtattt ttgatgatat cgttccaaat atcttatgtc ttggagcatt
     5281 tggcatagtg ttttatctat tgaagaaaga agtgaaacca ttaacgatct tgatcggact
     5341 agctttcttt ggaatttttg gttcgtggat agggattttt taaaaaagga gcagagaaaa
     5401 tgtttacgat gcaagattat atctatgaag aaaaggaagt actaagtacg atcttgaaaa
     5461 agaatgattt ttcgacaaga gaacatatga aaaaaacagt gaatttactg atcttagcaa
     5521 cgggttcatc ttataacgct tgtttagcag cgaagccagc attggaatct tatggtgatc
     5581 ttactgtcga tattcaagaa cctttttatt ttttcaatta tggaaaactt tctccttcaa
     5641 tcgatactgt gattgctgtg tcacaaagcg ggaaaagtgc ttctactgtt gaagcagtaa
     5701 aaatgataca aaaacaaggg ttacctgttg ttgctatcac aaatgacgtg caaagtccac
     5761 ttgctttaga agctgcacaa attattgatt taggtgccgg ggtagaatca gtaggcttcg
     5821 ttacaaaagg ctattctgcg actgttttac agttactact cttagggata ggaattggaa
     5881 taagtaaaaa taaaatcagc aaaaaaatag aacaagacta catgcaacag ttgcgcaaaa
     5941 taatcaacca tttaccagcg attattcaaa aaacggaaga attttttgat ggatatcaaa
     6001 gtttatttcg tttagcacag cgtttcgtca ccattggata tggtccaaac tggggaacag
     6061 ctaaagaagc agagacgaaa ttgactgaaa caatccgcgt tccgtcacaa ggatttgagt
     6121 tagaagctta tatgcacggc ccatacctgg aggcagatgc ttcgcatttg ctattcttta
     6181 ttgaagggga aagtgtaaat aaggaacgtt cgcaaaagtt gcaaagatat atgagtcggt
     6241 acgttggaga gactctcacc attaccacaa aaaaagcaag aaacgagaaa acacttggat
     6301 tagcaataga atgtgatgaa tatctgtctg tattagcgct tgtcgttccg tttcaactat
     6361 tcgcttataa aaccgcagta gctaaaggta ttgacttgaa caaaaagatt tttgaagact
     6421 ttgatacagt cttgaaaagt aaattataag aggtggaaat catgctgaaa ttcaatgaag
     6481 aagaacaaat caaagcaata aaaggcgcac tagctttacg tccgcaagta gaagaaatca
     6541 tcgataaact ttatgcggaa aaatttgacg ctgtctatta tctaggaatc ggtggaacgt
     6601 atgcttcctc gatgcaagct gtcacttata tgaatgggaa aagcaacttg cctgtctttg
     6661 tacagcatgc tgcagaatat tacactactg gaaacaaaag attaacaaaa gattctatcg
     6721 ttattttgtc ctctgttacc ggtacaactc aagaagtagt gaaagcagta gaacaaatca
     6781 agaaagtagg agcgacactg attggattta tcgataaagc gaacagtaag ttgtctcagc
     6841 tatgcgattt tgtggtcact tatccagctc ctggaacaga acaaatcaaa ttttttatgg
     6901 cagcagatcg attgatgtat ttgaatggag aattcgaagc ttattcagaa tactatgaac
     6961 agctcgaacg ttatttacca acagggttag tggaagcaga gaaaaaagca gatgcatttg
     7021 gcttatcatt tgcagaaaaa catcgacgtg atagtatgca ttattttatt ggagcaggga
     7081 atcaatgggg agctgtttat tcatatgcca tgtgttactg ggaagaacaa agctggcttc
     7141 catcgaaatc tatccatgct gccgagtttc ttcacgggac gttagagatc gtagaagaaa
     7201 caaccccagt cacattattt ttaggcgaag atgaacagag agtgttatct gaacgtgtgg
     7261 ctaaactttt gccaaaaatt tgcagcaatt atacattgat agacacaaaa gactatccag
     7321 tagaaggtat cagtgaaaaa tatcgcggac gtgttttgtc tttcttattg atgcatgtag
     7381 tgacacaacg catcgatgct catgtggagc agttgaactg ccatccatta gagatacgcc
     7441 gctactatcg tcagtttgat tattaggata gactggataa aaagaatata aaaaataaag
     7501 agagcagcga taactgctcc ctttattttt ttaaaatatt ggcatcaatt acgttataaa
     7561 acacattttt tgcattatct acagttcaac ttataatctg tttaatggga catgtgggtg
     7621 ccaaccattt tttgttatgc aatagtacta tttagttgtt gcatggatcc gtagtaaaag
     7681 tccaagtaca acgagttgcg ccagtttaca tagcgttttg tatccagaat gttgactaaa
     7741 acaaaagttc ctatgtcaac cgtcaacaga gacaatctgt ccgttgcagg acctgacaga
     7801 tttagaaatg ttcaatctgt ctcttatgta ttttaaatca atactcacta ggagaagttt
     7861 cctaacctcc taatagtaat gctggtagta acattgtcaa aactgcttcc tcttaggaac
     7921 aagcaaagca taacgagtgt aaaaataaaa tgtagactat tgtaacatat cattgattat
     7981 ttcaagtaaa aacgataaaa gaagtaaaca aacacttaaa aacaatggaa attagtccca
     8041 actttaagaa atgtatacta taatggaaat tatcaattat ttatttggag gaatatccat
     8101 gaaacaaaaa atcgcagttc ttggtcctgg atcttggggc actgctttag cacaaacatt
     8161 ggcagaaaac gggcatgatg tccgtatttg gggaaatgta cctgaacaga ttgatgaaat
     8221 caataagtat catacaaatc agcatttttt acctgatttg acgatcccag aatcgattat
     8281 cggatataaa gaattggcag aagcagtgga taacgcagat gctattttat ttgttgttcc
     8341 aacaaaagcg attcgttcag tcgcacagga acttattggt aaaatgaata ctaagccaat
     8401 cattatacat gcaagtaaag gactagaaca agatacgcat aaacgaatct cagaagtttt
     8461 ggctgaagaa attccagaag aaaaaagaca ggcaattgtt gtattatctg gtcctagcca
     8521 tgctgaggaa gtggcagtac atgatatcac aacaattaca gctgcaagta ttgatcaaaa
     8581 agcagcagcc tatgtacaaa aactgttcat gaatgattat ttcaggattt atacaaataa
     8641 tgatgtgatt ggcgtagaaa ctggtgcagc actgaaaaat atcattgcaa ttggtgccgg
     8701 cgctatccat ggattaggat tcggagatga tgcaaaagca gcaatcatga ctcgaggatt
     8761 ggcagaaatc agccgtcttg gtgtcgcgat gggtgccaat cctctgacat ttatcggttt
     8821 gagtggtgtg ggggacttga tcgttacttg tacaagtgtt cattctcgca attggcgtgc
     8881 cggtaacttg ttaggacagg gacataagct ggaagaagtt ctagaaaaca tgggaatggt
     8941 cgttgagggt gtggcaacga caaaagcagc agtagagcta gctcaacagc taggtgttga
     9001 gatgccaatt actcaaacaa tctataatgt tttgtataat ggagaagata tcaaaaaagc
     9061 agcaaaagaa atcatgttac gtgacggaaa aatggagaat gaattttctc ttcgttaaat
     9121 agatgatcaa tagttaatc
//
LOCUS       DAYYCY010000103         9040 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000103 DAYYCY010000000
VERSION     DAYYCY010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9040
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.103"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            105..221
                     /locus_tag="ACHAZB_001966"
     CDS             105..221
                     /locus_tag="ACHAZB_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HFM8614606.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(276..1958)
                     /gene="recN"
                     /locus_tag="ACHAZB_001967"
     CDS             complement(276..1958)
                     /gene="recN"
                     /locus_tag="ACHAZB_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFM8614607.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(1976..2425)
                     /locus_tag="ACHAZB_001968"
     CDS             complement(1976..2425)
                     /locus_tag="ACHAZB_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFM8614608.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            complement(2532..3350)
                     /locus_tag="ACHAZB_001969"
     CDS             complement(2532..3350)
                     /locus_tag="ACHAZB_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HFM8614609.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            complement(3359..4249)
                     /locus_tag="ACHAZB_001970"
     CDS             complement(3359..4249)
                     /locus_tag="ACHAZB_001970"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFM8614610.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            complement(4251..4475)
                     /locus_tag="ACHAZB_001971"
     CDS             complement(4251..4475)
                     /locus_tag="ACHAZB_001971"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFM8614611.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            complement(4480..5817)
                     /gene="xseA"
                     /locus_tag="ACHAZB_001972"
     CDS             complement(4480..5817)
                     /gene="xseA"
                     /locus_tag="ACHAZB_001972"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFM8614612.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            complement(5818..6672)
                     /locus_tag="ACHAZB_001973"
     CDS             complement(5818..6672)
                     /locus_tag="ACHAZB_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HFM8614613.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            complement(6769..7218)
                     /gene="nusB"
                     /locus_tag="ACHAZB_001974"
     CDS             complement(6769..7218)
                     /gene="nusB"
                     /locus_tag="ACHAZB_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFM8614614.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            complement(7211..7636)
                     /locus_tag="ACHAZB_001975"
     CDS             complement(7211..7636)
                     /locus_tag="ACHAZB_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFM8614615.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            complement(7882..8946)
                     /locus_tag="ACHAZB_001976"
     CDS             complement(7882..8946)
                     /locus_tag="ACHAZB_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFM8614616.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
BASE COUNT         2456 a         2048 c         1509 g         3027 t
ORIGIN      
        1 cctattattt aaaaaaaggg aagtttttat aaaaaagccc tcttgtggaa atggaaaaaa
       61 gattgtacac ttgaatcaac taattaatga cgaggttgaa aacaatgcgt gaaaaaaaac
      121 aaacaagtac ttttgctaaa gtaacaaaat ttgtcatttg gacaatgctg attttaacga
      181 ttggatctgt tgtattgact gctgtaatga gtgtcatgta aaaataaaat atagctatca
      241 gctgctgaaa aggacctgaa ccactgcgag gtggctcagg tcctttttgc gagtttcaat
      301 agttcttttg catgttcgag tgtgagtttt gtaatctcac ttccagccag catacgtgca
      361 atttcttctt ctctttcttt tgttttcaac tctgaaacag aagtttgcgt acgtccatca
      421 atcactgctt tcacaatata atattgatag tcggcgactg cagcaacttg aggcagatga
      481 gtgatgcaca aaacttgaga gtatttcgat atttgagaaa ttttatctgc gatggcctgt
      541 gcaactcttc cgctcacacc tgtatccact tcatcaaaaa taatacttgt cacaccttgc
      601 tcagaagaaa aaatagtttt caaagcaagc aacatccgag acagttcgcc accagaagcg
      661 actttcacca aaggcttcag tggctctcct ggattggtcg taatgtaaaa ttcgatttgg
      721 tcaaatccat catgattcaa ttgtctattt tcttctttta agaaacgtac ctcaaactca
      781 gtgttttcca tatacagact tttcaattca tgcagaatgg atttttccaa ttcttttgcg
      841 atttttttac gcgcttcatg aagatctgcg gcttgttgct gaagtagaag ctctttttgc
      901 tctagttcct ctttcatttt gtccaattga ccttctgaaa aatcagaaga agctagctct
      961 tcggtgattt cgtcatagta agccaatatt gactcaatgg attcgccgta tttacgtttc
     1021 aactgtcgga ttaattctaa acgttgcgta acttcttcta aacgactctc atctaactcc
     1081 aataaatcaa tctgacgact catatcgccg attgcatctt gcagcaaata gtaagcactt
     1141 tggatatttt cataaattgc ttcatactcc acatctagat gggcgatccc ttgcagttct
     1201 gaaacggcca agccaacacc atctacactg ttttgttcat tattcgtcag tgctccgtaa
     1261 cctgcagcta atccatcagc aattttttga taattgctaa gtttttctct ttcttctttt
     1321 aatttctctt cttctccgac ttgcaaatca gcagcagcta tttcttcttg ctggaaatgg
     1381 agcatatcta ttctctgaac ataggatttt tcattttgct gcacttttcg tacccttttt
     1441 tccagctcac gataagagat atattcttgc tgataactct ttttcttctg ttggaaagct
     1501 acatctccaa aacgatccaa taaagcaaga tgtgattcag gctggagcag ttcctgatgt
     1561 tcgttttgtc cttggatatc tactaaataa ctgccgattc ttctcagatt agctaatgtg
     1621 ataatatgac cattcactcg acaaacattt tttccagtta aggacatatc ccgtcgaaca
     1681 atcagattgt cctcatctgt ttcgatccct aattcaacca tcagttcaga gaatccttct
     1741 tgttttggta gttcaaacaa cccttcaagc acacattttt ctgctccttg acgtatataa
     1801 tcgcttgaac ctcttcctcc tgctaacaga cctaaagcat cgataataat ggatttaccc
     1861 gcaccagtct ctccggtcag cgcggtcatt ccttcgtgaa aagaaagccg caattccgga
     1921 ataatcgcaa agttagtaat actgatttct tgtagcatca cattcgctcc tcaacttaaa
     1981 ggtaatgcag gaagtctttc ttcaataaaa ctgcctggtc ttcagttcgt gtaatcatca
     2041 aaacattatc gtcatcattg ataatcgaaa ataattcttt cttgtaacgt ttatcaatca
     2101 aatttgctgc tgccgaagca tttcccggca atgttttcaa aataacaaat ttttccattt
     2161 ggtcaactgc cacgaaagcg tcttttaata gtttttccaa tttagtattt acatcttcgt
     2221 ttgtttcagc tggtatgcta taacggtagc cgccatctga agccggtacc ttgatcagtt
     2281 tcagttcctt tatatctctg gaaatcgtag cttgagtaac agatattcct ttgcttttca
     2341 ggatttcaac aaattcttcc tgctttcgga tatcttgctc actcaacaat cgagtaatca
     2401 aacgatgccg atcttttttt ctcattacgt cttcgctcct tagcatattc ttcttaatca
     2461 atgatagctt atttccttgg tataataaag aagaatccgc atacttctca cttgattttc
     2521 acagttacaa tttatttatg gtcaaactgt tcgtgtgcat ttgaaaccac ttctgccata
     2581 ttaatagctg aattgattgt tccgctttcg ggtactttcc tcaagtgagc caagaactcg
     2641 atatttcctt ctccccccgt gatcggagaa taacttaatt ccagcacatc atatccacct
     2701 tgtgccgcaa atgacaaaat gtcttctaaa actttttgat gggtctgagg gtcacgaaca
     2761 attccgtttt tccctacagc ttctctgcct gcttcgaatt ggggtttgat taaagcaatc
     2821 acttctccct cttcttttaa aatagcatgt aatggaggca gcatcagttt tagtgaaata
     2881 aaagaaacat cgataacagc aacttctgga attcctaagg taaagtcttc gggttttgca
     2941 taacgaaaat tcactctttc cattacgacg actctaggat cttgtcttat tttccaagct
     3001 agctgattgt agccaacgtc taaagcataa ctcatttttg cgccattttg caaagctgca
     3061 tcagtaaatc cgccagtgga tgcaccaata tctaacaatg tttttccttc aacagataga
     3121 tcaaatgctt tgagtgcttt ttctaatttt aatccccctc gagaaacata aggaagtttt
     3181 ttacctttga ctcttaattc actactcact ggaatttttt ctcccggttt atcaaaccgc
     3241 tcgttttttt cgttgtaaat caaaccagcc atcactgaac gtttggcttg ttctcttgtt
     3301 tcaaataatc cttgtttgac cgctaataca tctacgcgtt ctttttccat tctttccctc
     3361 atttctccac atcaaattgc ttgatcatct ggcgcatcag cccgccatca aaagaaaaaa
     3421 cgtcctcttc taatttgtct atgatcttgt tggcggataa cagctcaatc tccaacgcct
     3481 cacgtgtctt ttctaggcct aataaacgcg ggtaagtatt tttttccata cgttcatctt
     3541 taccagctgt tttccctaat gttttcgttg taccaatcac atcgagtaga tcatctctta
     3601 tctgaaaagc taaacctaaa tgttcggcta atcgttgaag tagcaataag atttcttcag
     3661 gttgcttcgc taatatgcca ccagccagca aggcataacg aatcaaatgc cctgtctttc
     3721 tttcatgaat aaatgctaat tcttctaaag aaagcttttt cgattcgccc tcgatatctg
     3781 cagcttgccc agcaaccata ccttgtgatc ccgcacaaac agctaattgc tgaagcagca
     3841 gaagcttagg agaattacct aagtgtgcca tactgattag ctggaatgct cctgtaagca
     3901 aaccatctcc ggccaatata gctaatgctt cgccgaatac tttatgattg gtaggttttc
     3961 ctctacgaag atcatcattg tccatcgcag gcaaatcatc gtggatcaag gaatacgtat
     4021 gcaccatttc taaagcagct gctacttgat agacgggaac atcaataggc tcatcaaagg
     4081 aagcaacggc cgttaaaagt aataagggac gtattctttt cccgcctgct ttcactgaat
     4141 aactcatcgc atcatagagt cctctatccg ttgtatgctc atctaaaaaa gacaagattt
     4201 ctttttctac agcaggtaga tgaatagctg aaaagtcagt taatctcatc tcagctttcc
     4261 tcgtttccgt caaaatcaac ttcttcattt gattctgtca tcattttcgt caaggtctct
     4321 tctgctttag ctaatgtatc ttggcaatgt ttgcttaatt ccattcctcg tttaaacgaa
     4381 tctagagctg attctaatgg aacgtctcct tgttctaact gcatgacaat tttttctaat
     4441 tcttgcagtg actcttcaaa agttggattt tccatatttt tattctcctt tgtctatctc
     4501 ttgtacttgt cctttaacga ttccatcttg ataatggatc gtcagcaaat ccccttgatg
     4561 aatctcggtg acacttttta ctacttgatc atttaacgtc gtatagctgt agcctcttcc
     4621 catgattttc aatggactca acaaatcaag agattgaaca gcttgagcaa aacgctgctg
     4681 ctgttgtttc atatattgtt ggattttctc ttctaagcgt ttttctagat aagcagtttc
     4741 ttggcgagct gctttaactc gataaagcgg agccacttgt tccaattggt ggcttaatgc
     4801 gatcgtttgt ttttctttcg catgataaat ttgctgtgta gcttgatata atcgctgttt
     4861 caactggtct aatttgatgg tctgaccttc atacaatctt tcaggctgcc gaaatacata
     4921 ggaacgtcga gcacgctcga accgttcttg tttgcgctgg atctggcgta caaacgcctg
     4981 ctgcaatcta gcttgccgtt cttgaatgcg catcaactct tctgacaaaa caggaacagc
     5041 taattctgca gctgctgttg gagtggcagc ccgaacatct gctactaaat cagcgatcgt
     5101 ggtatctgtt tcatgtccta cagatgaaat caccggcgta ttagcttgat aaatcgcccg
     5161 agcaacccgc tcttcattaa acggccataa atcctcaatc gaaccacccc cacgaccgat
     5221 gatcattgta tcgaaattgc ccatctcttc tactctctgg atattcctga caacattgtc
     5281 cgctgcttgg tttccttgta caactgttgg gaacaaaacc aattggacaa ttggatatct
     5341 tcttttcacc gtcgtgatga tatcgcggat aaccgctccg cttggactgg taaggaccgc
     5401 gattcgcttt gggaaacgtg gcagcatttt tttaggccct tgaaaaaggc cttccttcat
     5461 cagcttttct tttcgttctt ccaattcttg atataaagca cctattccat caggctccat
     5521 gtgctctaca tagatttgat aagaacctcc cgcttcatat aatgaaatac gcccaacgac
     5581 aaggactttc attccttctt ttggctggaa tcgtaatttt tggaatgctc ctttgaacat
     5641 aatagcagat atttttgcat ggtcatcttt taagctgaaa tattgatggg cattggcacg
     5701 taaacggaaa ttcgatattt cgcctgttaa atatacgcgt tctaaataag gatcggcatc
     5761 aaattttctt ttcaaatact tcgttaatgt agtgacagtt aaatattctt gtgccatcta
     5821 ttcacctgct ccaaaagctt cggcagattt tacggtttga tacatcagca tcgtgatagt
     5881 cattggccct acaccctttg gcacaggagt gatccagcct gcaataggct ccacttcatc
     5941 aaatttcaca tcaccgatca gcttaccgtt agcatcacga ttcatcccta catctatcac
     6001 tacagcacct ggttttacga attcttttgt gacaaaatgt ccacgtccta ttgctacgac
     6061 taaaatatct gcttcttgtg ccagttcagc cagattttcg gttttagaat gagcaacagt
     6121 aacagttgca tctgccatca agagtagctg agccatcggt ttaccgacga tattgcttcg
     6181 tccgataact actgctcgtt tacccgccgg atcgatagaa taagcttcaa acattttcat
     6241 gatgccgtat ggagtacaag gaatcttgtc cggtttaccc gcaaggagtc tgcctaaatt
     6301 catcggatga aaaccgtcta cgtctttttc agggcggata gctaacagga tcttttcttc
     6361 atctatatgc tttggcaatg gtaattggac caatatacca tgaaaactag gatcttgatt
     6421 gtatttttca atttctgcaa gtagttcgtc ttcagaaatc gtctcaggat aacgctctac
     6481 caatgaatgg attccgattt cttttgctac acgttcttta tttcttacat agatttggct
     6541 ggctggattt tcccctacca ataaaacgac taatccggga tggataccat tatctttcaa
     6601 tttttggatt ttttcagcaa tctctgcctg cattttttca gctaattctt ttccgttaat
     6661 caattctgcc atctaaccac tcccagatat tttttgtgtt ttcattctac catagaattt
     6721 tcccgattta taacaggttt atgatgattc atagaaaaaa gctaggactt acgccccagc
     6781 ttctaattct ttcaatacat tggataaaac accgttgacg aatttacgtg aacggtcatc
     6841 actaaatgtt tttgccaact cgattgcttc atttaaagca acagccgctg gtacatctgt
     6901 cacataagtc atttcaaaaa tcgcgatacg caagatcacc aaatccattt ttgagatccg
     6961 attgatgtgc caattgtttt ttaaatgttt ttcaatcact ttatccaatt catctttttt
     7021 tgcacagaca ccgccaacta gcgtatcaag ataaacagga acaaactctg attcatcttc
     7081 gttgatcatg tcgcggtggt ctaattcgat tgcgttgaaa atcgcatctt cttttgttag
     7141 gtctgcattg aagtctaatg gaaataaagc ctgcaatgcc atttcccgaa tctcgtgtct
     7201 tgataattct ttattcattc tcttcctctt cgtcttcaaa aagttcttcg attgttggtt
     7261 gttctaattt ttcaggaaca acagctacta catggatatt gacttcagat aaatcgatat
     7321 ccgtcatgaa aaggacttgc tggctgaccc gttcctgcat ttccattgct actttaggaa
     7381 ctgaaacacc atattctaga taacagtaaa gatctacttt caagccatct tcctcgtttg
     7441 ctaagtaaac accttttccg tgagctgcac gtcctaataa ttcagtgaca ttgctggcaa
     7501 aagttccgcg cattccgtat acgccttcta ctttagaagc tgcaatcccg ataattactt
     7561 cgatgacttc aggagcgatc acgatctctc ctaaatcttg gtttgcgttt aaaatcaaat
     7621 ttttttcatc tgccatttat ttattcctcc ctttgcctaa gcacgagata catccgaatt
     7681 ctctatgatg cggcatagtg atcacgtggt aagaaaactg ccgccgatca gctgcaaact
     7741 gctatgaatt ggagaaaaca cccaacttta ttatctctta cagcttaaaa gttcttctat
     7801 agtgtatcat taaatcgagt gattttctat aacaaagtct gtgaacactg atttttacgc
     7861 ttacttcttc tattgatgaa actataagat gatcaattct tttggtgaac gagtcaacac
     7921 acgatttcca tcttctgtga tcaacaagtc atcctcaata cgtactccac cgattcctgg
     7981 taagtagatc cctggttcat cagtgatcac atttcctggt acaaattgct tttctgcacg
     8041 agaagaaacg tttggtcctt cgtggatctc taaaccgatc ccatgtcctg tagaatgtcc
     8101 aaatgcttcc ccgtaaccat gttttgaaat ataatctcta gcaactgcat ctaattggac
     8161 accagtcatt cctggttttg cagcatcaat aactgctaat tgagcatcta agactatttg
     8221 ataaatttct tttaattttt ccccaggatc accaatagcg aatgtacgtg tcatatcgga
     8281 aacgtaccct tcgtaatagc aaccaaagtc caatgtgatc aagtcacctt gttcgatgat
     8341 ttttttgctt gctacaccat gcggcatagc cgaacgcaat ccgctagcaa cgatcgtatc
     8401 aaaagaaaca cctgaagcgc caagagaacg catataaaag tctagttgat ttgcgacttc
     8461 aatctcagtc atgcccggtt ggatcatttt caggatgtga tcatatgcca tatccgcaat
     8521 atgacatgct ttttcgataa tagcgatttc ttcttcatct ttcacttctc tgagttcttc
     8581 gatcatctgt gaaacaggta ctaattccgc atcgatgatt tcttctaaaa ccgaatattc
     8641 taaaaatgag actgttgttt cctcaaaagc taatgagtgc agttcttctt tgcgtactaa
     8701 gtttgctacc tcatcaaaaa taggtccgac atttttaata atttcaaacc cttgagcctg
     8761 cgctgccgcc tgctcggtat aacgaaaatc agtaataaaa aatgcttttt ccaaagtaat
     8821 aacagctaat cctgtcgtac ctgtaaagtt cgttaagtaa cgaagattgt acggactggt
     8881 aaccaaaaat gaatccaaat tttcttcttg catttttttg cgcaattttt ctacacgtag
     8941 catcataatg aaactccctc tttctttgca cgcttttttc cattatagca aacttttctt
     9001 cgtacacgac agttaaataa aaatttccgg aaaacttctt
//
LOCUS       DAYYCY010000104         8937 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000104 DAYYCY010000000
VERSION     DAYYCY010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8937
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.104"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(422..919)
                     /locus_tag="ACHAZB_001977"
     CDS             complement(422..919)
                     /locus_tag="ACHAZB_001977"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HFM8614617.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(959..1768)
                     /locus_tag="ACHAZB_001978"
     CDS             complement(959..1768)
                     /locus_tag="ACHAZB_001978"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8614618.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            2102..2959
                     /locus_tag="ACHAZB_001979"
     CDS             2102..2959
                     /locus_tag="ACHAZB_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFM8614619.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            2952..3854
                     /locus_tag="ACHAZB_001980"
     CDS             2952..3854
                     /locus_tag="ACHAZB_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFM8614620.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            3887..4237
                     /locus_tag="ACHAZB_001981"
     CDS             3887..4237
                     /locus_tag="ACHAZB_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFM8614621.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            4286..5209
                     /locus_tag="ACHAZB_001982"
     CDS             4286..5209
                     /locus_tag="ACHAZB_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFM8614622.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            5287..6180
                     /locus_tag="ACHAZB_001983"
     CDS             5287..6180
                     /locus_tag="ACHAZB_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM8614623.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            6218..6781
                     /gene="amaP"
                     /locus_tag="ACHAZB_001984"
     CDS             6218..6781
                     /gene="amaP"
                     /locus_tag="ACHAZB_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFM8614624.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            6795..6983
                     /locus_tag="ACHAZB_001985"
     CDS             6795..6983
                     /locus_tag="ACHAZB_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFM8614625.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            6996..7898
                     /locus_tag="ACHAZB_001986"
     CDS             6996..7898
                     /locus_tag="ACHAZB_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFM8614626.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            7930..8172
                     /locus_tag="ACHAZB_001987"
     CDS             7930..8172
                     /locus_tag="ACHAZB_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFM8614627.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(8428..8799)
                     /locus_tag="ACHAZB_001988"
     CDS             complement(8428..8799)
                     /locus_tag="ACHAZB_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8614628.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT         3116 a         1545 c         1731 g         2545 t
ORIGIN      
        1 aaccgtccgg tcgtaggttc gagtcctaca aggtacataa gaggttgtga aaaagctgcc
       61 ctgcatcaag taataagaac agccaagtaa aaacagcttc ttatgttttt acttggctgt
      121 tcttattact gcagatgcaa gcttttgaat accgtttaag gaagaggttg tgaaatcagc
      181 gttttgacct gagtattagc ttaacaaacg gaaaaatagt tcctcatatt ttgccgtttg
      241 ttaagctaat accgagggtc agctgattga acaccattta agaaagaggt cgtgagcctg
      301 ccgtttcact ctgtgtcata aaaaagccaa tctactttga tagaaaaagc acgtaggact
      361 aaaacagttc tgcgtgcttt ttttattgtg tgttaaatat cgtaaatgaa gatagatatt
      421 tttatgattc gacgatagag acgttgaaat ctttcaacag ctctaaagcg gattctccgt
      481 gttttctact ggagactaac attggatcaa caaaaaagtc tgcttcagga aaagcactct
      541 gcaggcctat cagattagag agtacacaca agtttgtttc aatcccgact acttcgatct
      601 ctttttgttc ttctttgctt tcaaataaag tttcctggat ttcagctaaa gtttcaggtg
      661 gaatcgtaaa gtaatacttt ttaatcactc gttcattttc ttgtggagaa agaagaggga
      721 tcaattgcaa atcttctcgt tcttcttctt tgtccttcag ttcaactggt atatcccgtg
      781 tgaagagaat atattcatta ttttggcgtg ctttagctaa tctttgagca atacgttctg
      841 ccaattgatc tgactctgga atataaaatt cgctgtttgg atcaagaata tgattttgca
      901 tatcaataat aactaacatt ttctttcctc ctacattcat atcgtcattt tattcttttt
      961 aatagaagtt ttttaaaata taagcaacac cagcttcttc attcgtcagt gttacttggt
     1021 ttgcaatttc ttttacttgt cgctgtgcat tccccatcgc aatactcaac ccgctgtaat
     1081 tcaacatagc aatgtcgttt tcttgatcgc caaaagccat gatgtcactc gattcgattc
     1141 caaggtaatc cgccagaaaa acaagtgaat gagctttagt gatacctgta ggcgtgattt
     1201 ctaagaaatg cggatcagag aatactgatt ggataccatc tgcttgcaga tctctttgta
     1261 gcttctgtag tttttcttca ctcttatctg ttaatgctaa tttaagatag ctaagagaag
     1321 gatcttccaa catatttaaa atttgatcaa aagaatggtt ctgtaaattg aaatgttcat
     1381 aataagggtc atctttgact agatttgatt catcgaatgt cagctgatca tcttgggttt
     1441 ctcggctagc aatgatggat attttcgctt cttgagcata gaagaaggct tttctcagtg
     1501 tatcgctatc tatttttgaa cgtctgagaa ctttcgccat ttttccatcc ttcatttcag
     1561 caatcagtga accattggaa gaaatcaaat agccaccgat tcctaattcg gaaaaaatat
     1621 ctttagcaga aatgctgttc cttccggtag cgacggttac gattccgcca ttttcatcaa
     1681 atttccgaag tgcttggata ttttcttctg gtatctttcc atttcgatca agcaaggtac
     1741 cgtctaaatc aatcgctgct agtttcattt tatcttccta ctttcttttt acatatctat
     1801 tttcctgtta ccttaagatt aaatgagaat ttaaaaaaat gaaactaact tgctttgtca
     1861 ttatgaattt tttcaaaaaa cattcttaag aaaaaggtgg ctatatatag tagtttttgg
     1921 tcaatacctt tgaataaaaa gccatgaaaa acgaatagtt agcgcttacc gatgcgtttt
     1981 gtgataaaaa aattttaaaa tattcaatga aaacaaagca tatattttgc tttaaaacca
     2041 aatgaggaat attctagatg tagaacaggt tagaaaaaga ttagaaaaag gagtgatttt
     2101 gatggagaag agatacggcg tagttcctgt ggtaactgct tcagacgaaa attatgctcc
     2161 ttacttgagc gtgatgatcg caactgcatt agaaaattgc aataaaacaa gacgcataaa
     2221 attttatgtg attgacgatg gtctatcaga atatagtaaa gaaggattgg aagaaacggt
     2281 aaataaatat tcaagtaatg caagtatcca attcctgaca gtggaaaaag atatctacga
     2341 agacttccta gtcagtgacc atatcacaac taccgcttac ttaagaattt ctttaccaaa
     2401 cttattagct aaagaggatt ataagaaagt cctttactta gattcagatg tgttagtact
     2461 agacgatatc gtaaaactat atgatgagcc gttaaatgga aaaacaatcg gcgctattat
     2521 cgatccaggc caagtgaaag ctttggaaag attaggaatc gattccgatg acttgtactt
     2581 caattcagga gtgatggtga ttgacattga tcaatggaat aaaaaagaaa ttacagaaaa
     2641 aactatccat tacctcagtg aaaatggcga tcgaatcatc tatcacgacc aagatgcttt
     2701 gaatgctgta ctttatgaag attgggaaca acttcatcca aaatggaaca tgcagacttc
     2761 gctgattttt gaacgtcatc cagcaccaaa tgaaaaatat gaacgtcagt ataaagaagg
     2821 aaatgaaaaa ccttcgatcg ttcactttac aggacatgat aaaccttgga atacgttgaa
     2881 agatcatccg tataccaatt tatacttaaa aaaattagca cacagtactc taacgaaagt
     2941 aggcgagatc aatgaataaa aaagaaatag cagtagtcgc aagttgtaac acaaaattcg
     3001 taccacattt agctgcatta tttgtctcag tattagataa ttgcaacccg tctaaatttg
     3061 ttcggttcta tgtgattgac gatgatatcg attttgaaag taaacagctt ttacgcttct
     3121 cagtgaaaaa cgcacgaatg aactcagatg tggaattctt gaaaatcaat aaagaattct
     3181 ttacaaatgt ggtaatcagc gatcgtattc cagaaacagc ttattatcgt attgcgattc
     3241 cggaattgtt ccgcggcaca gaagttgaac gtatcttata tatggattgc gacatgatcg
     3301 ctttacaaga tatctctaaa ttatggcgtc ttgacttcgg tgattcaatc gtagcagctg
     3361 ttgaagacgc tgggttccat caacgactgg aaaaaatgaa aatttcagct aaatcaatgc
     3421 gttatttcaa ctcaggtttg atgctgatca atgtgaaaaa atggttagat gaaaatatta
     3481 cgcaaaaagt attagatttc atcgaacata atccagaaaa attacgattc catgaccaag
     3541 atgcattaaa tgctatctta catgaccgtt ggttaccact tcatccgcgt tggaatgcac
     3601 aaggctatat catggcaaaa gcaaaaaaac atccaacagc agcaggtgaa cgtgaatacg
     3661 aagaaactcg caacaatcct tacattatcc atttttcagg acatgtgaaa ccgtggagca
     3721 aggattttga agggccaacg aagaaatatt acgaaaaata tgcagggatg acggctttcc
     3781 gttgtgtagc taaattccct aaatatccaa aatatgcaaa agttcaacca aaaacagatg
     3841 tagttgttaa ataatagaag acaacaactg aagaggggga gtaggaatgg aaattacaat
     3901 gtctaaaaat gcagtagaga cgttaattga aaaggttggg gaaaatacaa aaatcgcact
     3961 cgcgcttatc aatgattccg atccatttct aagggacaaa ggtgcttttg caaaaggcag
     4021 cttttttcaa atcattcctt ttgtttcgga atttggagaa tatgcgacta aaatcgagca
     4081 tccattattg gacatctata catctaaatt agagcaaaat tatttcggta aacgattgaa
     4141 catggacttc aacaaacagc ttgacagctt ttcattagaa aatgaaatcg cagtactcga
     4201 ttataatatc aaattaaaga actgcttctt cagctaatag tttcgagaaa gtccaaaaag
     4261 gaggtataca ttgagatact actttgtgga tgaagaaaag aatgtaatag aaacagatga
     4321 aaaaaaatat aattggctcg tacttgatag tgcaaagccc gaagagattg agcaagcaat
     4381 cgaagaattc gagcttcctg atgatatttt cgtaggaacc gaatatccgg aagaagtttc
     4441 tcggatggag cgcctttatg agacgaaact agagcatcca ttttctttgg tagtgattaa
     4501 tctagatcag tcagataatc gtatcgaaaa aaaattaacg ccaatatctt ttgtactttc
     4561 cgataatctc ttgattactt gtatcgatag aaaaaccgaa tttgtggatc ggctgctgac
     4621 aaagcatgga aatcatttaa actcgtttga aaagatcatc acgtatacat tattagatat
     4681 ttacacccat tacgtgaaag aactaagaga gatgaaaaag cgaattgacg ctttggataa
     4741 agcagctcgt aaaacaacgg aaaatgaaga actgtataaa caggcagatc ttgaaagaga
     4801 catcgtatat atcgatcata ccttaagaga tcaacgggaa acaatggatc aattatggaa
     4861 ttctgaaact ttcatcaaac gtttagacga tgaacagcta ctctatgatg tccatttgag
     4921 acagcgacaa acagaaaaaa tgatcgagat ctatcgagat ttattagaga gtattggtga
     4981 tctattcaac gggatgatgg acaataatct aaatcatatc atgaaatatc tagattcagc
     5041 tgcattaatc atttccgttc cggcgttgtt cgcagggatt tggggaatga atacaggagg
     5101 attaccgggt aaaagttctg ctctaggctt tatactcgta gtctttggct ctttgatagc
     5161 agcagtagtt ttaggttacc atttataccg gaaagattat acaaattaaa gaatctattt
     5221 aaaacaggta tcaaacaaag aaatttgttt tggcaataca atttaaaaac taggaggaat
     5281 acagcaatga cagaaccaaa attaatcgac ccgcgaaact tgtaccatac ggagaaattt
     5341 cctaaacaag atcaggatac accagcttta cagcagaata tgattcctgt tcctgattgt
     5401 ggagaagaat cctatgaagg caataatcaa ctagaaaacc gccgtgtact aatcactggt
     5461 ggagattcag ggatcggtcg tgcagcagcg attgcatttg ctagagaagg ggcagatatc
     5521 gctcttcaat tcttcccagg agaagaaaaa gatgcagagg aagtagcaaa atacgtgaaa
     5581 gatgcaggac gtaagattgt cttactacct tttgatcttc gtgacgaaca agcacctaaa
     5641 gaaatcatag ataaagccgt gagtgaactt ggcgggctag atacgatggt actaaacgca
     5701 ggacagcaaa tttcttgtca gtctattgct gagttaccac ttgagcaagt caaagacaca
     5761 tttatgataa acatcatcgc tatgtttgct ttagtgaaag aagctgttcc gcatatccca
     5821 gcaggaggag caatcgtaac aacaacttct gttcaagcat tcaatccaag cgaacattta
     5881 ttagattacg cagcaacaaa agcttcaatc gctaacttta cgatcggttt agcaaaacag
     5941 cttgcaccta aaggaatccg tgtaaatggt gtagcacctg gtccaatctg gacaccatta
     6001 caactagacg aaggacaacc ttctgattcg atccctgaat ttggtcagca aagtttacta
     6061 gaacgtgcag gtcagccagg agaattagca ccagtttatg ttttcttagc atcgaataaa
     6121 gcaagttatg ttacagccca aatttacgga gtaactggtg gagaagcgat caacttataa
     6181 gaagttgatc gtttcacccc tatgaggagg tgcgaaaatg aataaaggtg taaaaacgat
     6241 cggagtgatc ggaacattac ttttgctatc tgtgcttttg tttgcgatac tattaagtgc
     6301 aacctatcca ttgccttttc agctagagaa gtttcgattc ttttccatca ctaattcata
     6361 cgtgcaacaa tatgtatttt ggcttgccgt tgcatttgct attttgacaa ttatcacgtt
     6421 acttgttttc ctgttttatc caaaaacaat cggaaacttt ctactgaaac atggtgatgg
     6481 ggagttgacg ttggataaaa aagcaattga aggaatggtt cgttctcact tgcatgaaga
     6541 tgaattcatc cattcaccaa aagtaaatgt caaagcaacg aaaaacaaga tcgccgtcaa
     6601 tgtaaaaggg gagttgaaac gaacttcagc actcattggt aaaacaggaa cattgatgaa
     6661 agaaatcgaa aatgaagtac aacacatatt gggaacaaaa gagccagtga aagtatcggt
     6721 gaaatacaca ggctttgaag aaccagctaa acgaagcaat gaccgcaaac gagtagaata
     6781 gggggggacc aaaaatgaaa gaactatttt cgacatacaa gctccctatc atctttgggg
     6841 tacttggatt gatattagcc gttttatgga tcaccgtggg ctttttcaag accttattga
     6901 tcttgatcat ggcagcgatc ggcgtcgcta tcggttttta tctgaaacaa acgaaaatct
     6961 tagatcacta ttttaaatct taggaggaaa catccatgaa taacaataac aaaaataata
     7021 acaataaaat gaacaaccaa aaaccagtgt ctgcacatcc agacccaacg acaccaaacc
     7081 aatcaaatgg tgtgaacgat gaaaagaaca aaacagcacc agtatctgca catccagatc
     7141 cagtagtccc taacgttcaa caaggagtag aagagaagaa acatgatgca gaaaagcatg
     7201 cacctcatca tccaggaact ccagaatcta aagttccaga acaacatgat gcaatcgatc
     7261 cttctaaacc tgtggaagag caaaaacatc cttctgacca tgcgaaagca caagaaaaac
     7321 ttagttctga ccacacaaac ggacaaggaa aacttggttc tgatcataca aataaccaag
     7381 gaaagcatga ttctgaacag gaagattcaa tgcgcggtga gctgaattat gaagataaag
     7441 ttgtgcaaaa aatcatcgga atcgcaattg aacaagtaga cggtttatta tctgcaaacg
     7501 gtggtttctt ctctaatgtg gcaggaaaac ttgtgaatac agataatgta acggctggga
     7561 tcgaaacaga agtcggtaaa aaacaagtag cagttgatat ggatgtcatt gtggaatatg
     7621 gcaaagatat cgaaaaaatc tttgaagaaa tgcaagaagt aatcggcaaa gaagtaaaaa
     7681 atatgacaca tttagaagtg atcgaagtca acgctaacgt agtggacatc aaaacaaaag
     7741 aagaatttga aaaagatcaa gaaacggtgc aagacaaagt aactgacgca gcgaaaaaaa
     7801 ccggtgaatt tgcttcgaat caaacagata aagtaaaatc agcagctgga agcggcgcac
     7861 aaaaagtaaa agaaaataca gaaccacggg ttcagtaata aggaagtaaa acattaggag
     7921 gaacgaaaca tgggatttat ttggtcactt atcgtcggag gaattatcgg agcaattgca
     7981 ggagcaatca cgaataaagg ttcttcgatg gggattattg caaatgtcgt agcaggttta
     8041 gttggatcag cacttggtca agcattatta ggttcttggg gaccaagtat tgcaggaatg
     8101 gcaatcgtgc catcattgat tggtgccatc attttagtac taatcgtatc attctttgct
     8161 cgaaaactgt aaaaagactt ttaaaaagag gtcgtgaaaa agctgttctt catcaagtaa
     8221 taagaacagc caaacaaaaa tagcttctca tatttttcat ttggttgggc atattatcga
     8281 agatgcaagc ttttgaacac tgtttatacg gaataagagg ctgtgacaag acttttgtca
     8341 cagcctctta tttatgcggg caaattccca attagatttt taaaagtttt tccttaaatg
     8401 tgtctttatt gatacagaga agcttcttta ataaaacaat gcctccctaa attttcttga
     8461 gataggaaag gtgaacatac aaaataatac gaccactttg aaaacccaga tgatccacaa
     8521 atggatcgta tgatactgat gaagtaaaaa gcaatggatc agaaactcaa agagattgaa
     8581 aaagacaagg aagaaataat atctcttttt tactcttgtc tgttttagcc aataatagct
     8641 cgtaaagtaa tctagacaaa caaatagaag taaacaaagt ttcgtaagag cagcaatcgt
     8701 atcatgttgc tctgctagac gattgacgag caggaaaaat gttggtaatg gaatatagat
     8761 cacgagtaat gaaagaaaaa agtaaacaat aaatggcata ctattcctcc taattgtgtg
     8821 agttcgccct cattttaaaa taacgatatt tgtttgtgaa gctttttcgt gtcgtaacaa
     8881 atgttatcta atgtattttt aaataaataa tcattattat caaaataatg ttaaaaa
//
LOCUS       DAYYCY010000105         8838 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000105 DAYYCY010000000
VERSION     DAYYCY010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8838
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.105"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..567)
                     /locus_tag="ACHAZB_001989"
     CDS             complement(<1..567)
                     /locus_tag="ACHAZB_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM8614629.1"
                     /translation="MNHKLSETEQYLWNFIEHLILEIPNYSIVKLSEQANVSTATIVR
                     TMKKKGYEGFTSFKHHLKEGENKNINFSFLDKVDKGIRRAILKNEQEVVRTINMLEIG
                     NIEDAIQRIKFADRVFVFARGFSEMIGQEMLVKLQLTGKNCQMHTDPEIIKSISRKLT
                     KKDSVIFVSLNGEKTNKNIKNKIYVFIFQ"
     gene            709..1641
                     /gene="pfkB"
                     /locus_tag="ACHAZB_001990"
     CDS             709..1641
                     /gene="pfkB"
                     /locus_tag="ACHAZB_001990"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFM8614630.1"
                     /translation="MSIYTCTMNLAIDLFIETDEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQQKQIQTEFIEVPGMTRINVFTQVNQTGEE
                     YKLVNQGPEIPQTAVHNFLNQIRALQAGDYLCVSGSLPRGLSPKVLIEISRICLIIDS
                     SDQEVMDCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVESGAENVLLSLGE
                     KGAILFTKDRVIRGNSPKGKVVNTACAGDAMLGTFLAGYMNRRSLDETLRKSIAAGSS
                     TAFRKGLTDFLDVEELSKQIKIKEERFERWENIN"
     gene            1623..3086
                     /locus_tag="ACHAZB_001991"
     CDS             1623..3086
                     /locus_tag="ACHAZB_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFM8614631.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEALEQAARKKGITIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFVGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTVLADNEAADTLEPEAISGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSSDPDHAQYNATAAMVKAVDDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVAM
                     NGGTGFLGGILAGFLAGYFILFLQKLLIILPKQLDGLKAIVLYPVIGVAFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEYSNPLVLGVIVGCMCAFDMGGPVNKAAYVTGTALLTQGN
                     TTFMAGASAACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKRDNKATNRTVIEEGRAGGLS"
     gene            3083..3541
                     /locus_tag="ACHAZB_001992"
     CDS             3083..3541
                     /locus_tag="ACHAZB_001992"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFM8614632.1"
                     /translation="MTIIHEENIFLQQPLDNQEEAFKFLAKQTFCLGIAHDEKEVYQK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSLIIVTLKKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRAAKSKIEIATILTKEIGEDI"
     gene            3538..4512
                     /gene="lacD"
                     /locus_tag="ACHAZB_001993"
     CDS             3538..4512
                     /gene="lacD"
                     /locus_tag="ACHAZB_001993"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFM8614633.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPEYGLPAAALREKECGLLLAYEKTGYDAKMPGR
                     LPDLLNSWSVKRLKEVGADACKFLLYYDVDEPVEINVQKQIVIERIGSECEAEGIPFF
                     LELLSYDATIGDPSSLDYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QDTCYSQAEASAYFYEQSKVTEQPFIFLSAGVSAALFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLLEETATSIYVNK"
     gene            4953..5681
                     /locus_tag="ACHAZB_001994"
     CDS             4953..5681
                     /locus_tag="ACHAZB_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8614634.1"
                     /translation="MESSNKGKSLYYQLVDLLKDRIETQMTPHDKLLSERELCQHYGV
                     SRTTVRLALQELENQGYIYRRHGKGTYVSDLSDKSSNLAGAYSFTEEMREIGRVPKTV
                     ILEFRQMEANKRIAKNLNIHLGETVFKLKRLRLADEEPLMVERSYLPAKLFMGLDLPL
                     LESKPLYDVFSQDYEQQVQLADEEFYASIVQKKDAVVLRIADGAPVLHLTRITYNVKN
                     EIIEYTDSVARADQFHYKIRHIRK"
     gene            5699..6895
                     /locus_tag="ACHAZB_001995"
     CDS             5699..6895
                     /locus_tag="ACHAZB_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM8614635.1"
                     /translation="MFELSKNKLKQIGAAITTQEIKQQPDLWKETIDRLMQSQENLAL
                     FFDQIKKEANGRRIRVIFTGAGTSQYIGDTLLPYLNEHGDNQFFIFHSFGTTDIVASP
                     KEFLFPNEPTLLVSFARSGNSPESVAAVQIANEQVETLFHLTITCAKEGMLAKNAQDD
                     KQNYLLLMPERANDAGFAMTGSFTCMLLSALWIFDQTAMEKKERYLEQLVKIGQDLLS
                     RDQEIQKLLPDFFERIAYLGSGSLAGATREAQLKILELTAGKVATIFDSSMGFRHGPK
                     SFLNSETLVVGFVSNQPYTRNYDLDILEEIKEDRVTANVIAIAQPGTIDFSGMTVLLE
                     DSVSKLPDAYLALAMIMTAQIIALYTSIKVANKPDTPSPTGTVNRVVKGVIIHKYETE
                     GEMTDE"
     gene            6888..7883
                     /gene="lacD"
                     /locus_tag="ACHAZB_001996"
     CDS             6888..7883
                     /gene="lacD"
                     /locus_tag="ACHAZB_001996"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFM8614636.1"
                     /translation="MNEEKNRAMERLSTKDGIISALAIDQRGALKKMIKSLGKEPSED
                     QISEFKELVSAELTPYASAILLDPEYGLPAAKVRAKETGLLLAYEKTGYDATTPGRLP
                     DLLADWSSLRLKEQQADAVKFLLYYDVDEAPEINHLKHVFIERLGSECEGENRPFYLE
                     LVSYDAQISDSSSPEYAKVKPHKVIEMMKEFSDPKYKVDVLKVEVPVNMNYVNGYSTG
                     EFIHSKEEAAAFFKEQSEATPLPFIFLSGGVSTELFQQTLIFAHESGSTFNGVLCGRA
                     TWKDGVKAYVENGKDGAIHWLRTNGRENIETLNKVINETATPWFNKTKSKKVKMK"
     gene            7880..8821
                     /locus_tag="ACHAZB_001997"
     CDS             7880..8821
                     /locus_tag="ACHAZB_001997"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HFM8614637.1"
                     /translation="MITTVTLNPSIDIAYTIEELNLDKVNRVETVNKTAGGKGVNVAR
                     VLNLLGEEVIATGLIGGQHGEYLKNQLDQLGIMHQFVPIKGETRDSIAILHSSRQTEL
                     LERGPFITKKEQQTFTDHFEQLLEISDIMTISGSLPQGCPLDYYKQLVALADKKGKDI
                     LLDTSGKFLLEVLEGKEKPLLIKPNLEELGELIGQRICSSIQLEKIQSLLAHPIFEGV
                     PWVVVSLGERGAVAKVAQCFYEVIIPKISVVNPVGSGDATLAGSAHALSKRQSIEEIL
                     AFSMACGMANAQQEKTGWIDPMKVTMLMKHIQVKKIK"
BASE COUNT         2893 a         1406 c         1952 g         2587 t
ORIGIN      
        1 ttgaaaaata aagacataaa tcttattttt gatgttttta tttgttttct caccgttcaa
       61 agaaacaaaa ataacgctgt ctttcttagt tagtttccga ctgatgcttt tgataatttc
      121 tggatcagta tgcatctgac aattttttcc cgttaattga agttttacca gcatttcttg
      181 gccaatcatt tctgaaaatc ctcgggcaaa gacaaaaacc cggtcagcaa atttgattct
      241 ttgtattgca tcttcaatgt tcccgatttc caacatattg attgtacgta ccacttcttg
      301 ttcatttttt agaatggctc ggcggattcc tttgtctact ttatctaaaa atgaaaaatt
      361 gatgttcttg ttttctcctt cttttagatg atgcttaaac gaagtaaacc cttcatagcc
      421 tttctttttc attgtccgca caatggtggc tgttgagaca tttgcttgtt cacttagttt
      481 cacgatggaa tagttaggga tctctaggat gaggtgttcg ataaagttcc agaggtactg
      541 ctctgtttca cttaatttgt gattcatatt ctttccttct ttcaccaaaa gttagttaaa
      601 aaaagcacga cgagtttata aatgtaaagg ttttctttgt gttgggacga ttaagaaaac
      661 attttctatt tcgcgtattt cataatagaa tcaaggaggt cgttggttat gtctatctac
      721 acatgcacga tgaatttggc aattgatcta tttattgaaa cagatgagat gcatcctttt
      781 atggtaaacc gcacaaaaga ggatgacatt caggcaaacg gaaaaggggt gaatgtgtca
      841 ctagtattaa agatgctaga tttcccaagt acggcattag gtttttctgc tgggttcaca
      901 ggaaagtata tagaagacta tttacagcag aaacaaatcc aaacagagtt tattgaagtg
      961 cctggtatga cgcgtatcaa tgtatttaca caagtcaatc aaacgggaga agaatacaaa
     1021 ttagtgaatc aaggacctga aataccgcag acagctgttc ataatttttt aaatcaaata
     1081 agagcattac aagcagggga ttatctttgt gtttctggga gtttaccaag agggctgtct
     1141 ccaaaggttt taatagaaat cagtcggatt tgtttgatta ttgatagtag tgatcaagaa
     1201 gtcatggatt gtttacccta tcgaccgttt ttgctgaaac caaatgagga agaactttct
     1261 tcttggttcg gacgaaaaat ggttacagat gaagattact ttgtttatgg acaacgacta
     1321 gtagagtcag gcgcagaaaa tgttttgtta tccctcggtg agaaaggagc gattcttttt
     1381 acgaaagaca gggtgattcg aggaaactcg ccaaaaggaa aagttgtcaa tacagcatgt
     1441 gcaggtgatg ccatgttagg cactttttta gcaggataca tgaatcgacg atcgcttgat
     1501 gaaacgttac gtaaaagcat agcagcaggc agctctactg cttttcgtaa aggactgact
     1561 gattttttgg atgtggagga attaagtaaa caaatcaaaa tcaaggaaga gaggttcgaa
     1621 agatgggaaa atatcaacta attgcagcaa ccggttgtcc gacaggaatt gcgcacacct
     1681 acatggcgca agaggcatta gagcaagcag ctaggaaaaa aggcatcacg atcaaagtgg
     1741 agacccatgg acaaattggg atcgagaatg aattaacggc tgaagaaatc aaacaggcag
     1801 atgctgtcat tattgccgca gacaaagatg tccattcaga aagatttgtg ggaaaacgag
     1861 tcattgatgt ttctgtcagt acaggaatca aggatgcaca acggctgatt gacgaagcat
     1921 tggcaggaaa aggtactgtc ttagcagata atgaagctgc tgatacttta gaaccagaag
     1981 cgataagtgg tgctaatttt ggtcgtagca tctataaaaa tttgatgaac ggtgtctctc
     2041 atatgttgcc tttcgtggta gctggcggag tattgattgc tatttcattt gccgtttggg
     2101 gaatctattc ttctgatcca gatcatgcac aatacaatgc tacagcggca atggtaaaag
     2161 cagttgacga tgcagcaatg ggaatgatgg ttccagtttt agcagcttat attgcagaag
     2221 ggatcgcgaa acgtccaggg ttagtcgttg gatttgttgg tggaatcgta gccatgaatg
     2281 gcggtactgg atttttagga ggtattttag ctgggttctt agcaggttat tttatcttat
     2341 ttttgcaaaa gctactgata attttaccta aacaactaga tggcttgaag gcaatcgtct
     2401 tatatccagt tattggtgta gcatttattg gcatcgtgat gtctttgctg gcaggtccaa
     2461 tgaaggctat aaatgaagga atgatgagct ttttagctgg atttgaatat tcgaatccac
     2521 ttgttcttgg cgtgattgtt ggctgtatgt gtgcgtttga tatgggagga ccggttaata
     2581 aggcggcata tgtaacagga acagctcttt tgacacaagg aaacacgact tttatggctg
     2641 gtgcgtcagc agcttgcatt gcaccaccgt tgattactgg ttttgctact ttattgtttg
     2701 gtaaatattt tgatactaat gatcgtaatg caggtttggt taattttatt ttaggatcaa
     2761 ctcatattac ggaaggagct attccgtttg ctgcgaaaga cccgatccga gtattaccaa
     2821 cgatgatgtt aggctcatca attgctgctg tcttgaccta tctgtttggt gtccaagtac
     2881 cagccccaca tggcggtttt ttagtgttac ctgtagtgac gggaaaaatc caatgggtta
     2941 cagcgattct tattggttcg atcgttggtg gagtgatact aggtttgatc caaaagaaaa
     3001 gagtaactga taaagacaag aagagggata ataaagcaac aaatcgtaca gttatcgaag
     3061 aaggaagagc aggaggttta tcatgacaat cattcatgaa gagaacattt ttttacaaca
     3121 gccattagat aatcaagaag aagcatttaa gtttttagca aagcaaacat tctgtttagg
     3181 aatcgctcat gacgaaaaag aagtatatca aaaacttctg gaaagagaaa aagaaggtac
     3241 tacaggaata atgtcaggtt ttgctatccc tcatgcgaag tctcaaacaa tcaatgaacc
     3301 tagtttgatt atcgtcacac tgaaaaaagg aattgaatgg aacagtttag atagacagtc
     3361 agttgatttt atttttgcgt tatttatccc agattcagaa gcaggaacga ctcatttgaa
     3421 attattgtct ctcgttgcac gatcattgat gcgaaaagag gttacagaac agttgagagc
     3481 tgcaaaatca aaaatagaaa tagctacaat attaactaaa gaaataggtg aagatatatg
     3541 aagaaaatga gtatagaaaa acaggaacgt ttgaagcgat tgacggatga agctggaatt
     3601 attagcgcgc tagctatcga ccaacgtggt gctttgaaga aaatgatcaa ccaatataaa
     3661 gaagctacat cagaagatat catccaattt aaacagatgg tttctcagta cctgactccg
     3721 tatgcttccg ctattttact cgatccagaa tatggtctgc ctgcagcagc gcttcgagag
     3781 aaagaatgtg gtttgttgct agcttatgaa aaaaccggct atgatgcgaa aatgccaggc
     3841 cgactaccag atttactgaa tagttggtca gtgaaaaggt taaaagaagt gggtgcggat
     3901 gcttgtaaat ttttattgta ctatgacgtt gatgaaccag tagagattaa tgtgcaaaaa
     3961 caaatagtta tagaacggat tggttcagaa tgcgaagcag aaggcatacc tttcttttta
     4021 gagctccttt cttacgatgc aactattggc gatccgtctt ctcttgacta tgcaaaagta
     4081 aaaccgcata aagtgattga agccatgcga gaattttcaa aagaacgtta tcaagtggat
     4141 gtattgaaag tagaagtacc agtgaatatg agatttgtgg aaggatatgg gcaagatact
     4201 tgttatagtc aagcagaggc atccgcgtat ttctatgaac aatcgaaagt gactgaacaa
     4261 ccgtttattt ttttaagtgc gggagtcagc gctgcactat ttcaagatac attgcgtttt
     4321 gcaaaacaat caggttcgac attcaatggt gtcttatgtg ggcgagcaac atgggcgaag
     4381 ggagtagcac cttttgttac cgaaggcgaa gaaaaagcga gtagttggat gttagaagaa
     4441 ggaaaacaaa atattaaaga attaagtaca ctattagaag aaacagcaac ttctatttat
     4501 gttaacaagt aagagaaagg attgtctgag tgacagattc aaaaaataat tagttattta
     4561 aaaaaatgtt tgtcaaattt tatagaaaaa aagtataaaa ctgcaaaagt tatctaaaac
     4621 taataataac atttgtcatt aataagctat ttttagttgt tgtaaatgat attggaagta
     4681 ataaaacgat agttgtagga atatcatagg gattaatgtg gatcacgctg ctcgataagt
     4741 tagaccattt tgacggatcg cttatgtggt tcgtcttttt ttctgttctt ttattggtaa
     4801 ctggtatttt tgaggtatct ttgtctattc aagatataaa gctttaaaga gctgttataa
     4861 tagctttttt ggtctaaaaa acctttgaca aatgattaat aattcactat tcttgtgttg
     4921 tggttataac cagtaaagaa gaggtgattt ttatggagag ctcaaataaa ggaaaatcat
     4981 tgtattatca actagttgat ttattgaagg atcgtatcga aacacagatg actccgcatg
     5041 ataagctgtt atctgaaaga gaactttgtc aacactatgg ggtgagtcgt accaccgtac
     5101 gtctggcttt acaggaattg gaaaatcaag gttatattta ccgaagacat ggaaaaggta
     5161 cctatgtctc tgatttgtct gataagtcat cgaacttagc tggagcatat agctttacag
     5221 aagaaatgcg cgaaataggg cgcgtcccta agacggtcat tttagaattt agacaaatgg
     5281 aagctaataa gagaattgct aagaatttga acatccattt aggtgaaact gtatttaaac
     5341 ttaaaagatt gcgtttggca gatgaagagc cattgatggt tgagcgttct tatctaccgg
     5401 cgaaactatt tatgggactt gatttgccgt tacttgaaag taaaccgttg tatgatgtgt
     5461 tttcacagga ttatgaacaa caagttcaat tagctgatga agagttttat gcaagtatcg
     5521 tgcaaaaaaa agatgctgtt gtgctacgaa tcgctgatgg cgctcccgtc ttacatctga
     5581 cgaggataac ctataacgtg aaaaatgaga ttatcgaata tacagatagt gtagcaagag
     5641 cggaccagtt tcattataaa attcggcata tccgaaaatg attgagaggg agaacaacat
     5701 gtttgagcta tcgaagaaca aattaaaaca aataggtgca gcaatcacga cacaagaaat
     5761 aaaacaacaa ccagatctct ggaaagaaac aatcgataga ttgatgcaaa gccaagaaaa
     5821 tttagctcta ttttttgatc aaataaaaaa agaagcaaac ggaagaagaa tacgtgttat
     5881 ttttacgggt gcaggaacgt ctcaatatat tggggatacg ctcttaccct atctaaatga
     5941 acatggagac aatcagtttt ttatcttcca cagttttggc acgacagata ttgtagcttc
     6001 accaaaagaa tttttgtttc caaatgaacc gactttatta gtttcatttg ctcgcagtgg
     6061 caacagtcca gaaagtgtag cggctgtgca aattgcaaat gaacaagttg aaacattgtt
     6121 tcatttgacg attacttgtg ctaaagaggg gatgttggca aaaaacgcac aggatgataa
     6181 acagaattat ctgttattga tgccagagag agcaaacgat gcaggttttg ctatgacagg
     6241 aagttttacg tgtatgttat tgagtgcttt atggatcttt gatcaaacag caatggagaa
     6301 aaaagaaaga tatcttgaac aattagttaa aatcggtcaa gatcttcttt caagagatca
     6361 agaaattcaa aagttattac ctgatttttt tgaacggatc gcttatctgg gttcaggaag
     6421 tttggcaggt gcgacaagag aagcacaatt aaaaatttta gaattaacag caggtaaagt
     6481 tgctacaatt tttgattctt cgatggggtt tcgtcatgga ccaaagtctt ttttaaatag
     6541 cgagacactc gttgtaggtt ttgtgagcaa tcaaccatat acgcgaaatt atgacttaga
     6601 tattttagag gaaataaaag aagatcgggt cactgccaat gtcattgcga ttgcgcaacc
     6661 gggtactatt gatttctcag gtatgactgt gctattggaa gactcggtga gtaaattgcc
     6721 cgatgcctat cttgcattag cgatgattat gactgcacaa atcattgcac tctatacttc
     6781 aatcaaagta gctaataaac cagatacacc atctccaaca ggcacggtca atcgagtagt
     6841 caagggcgtc attattcata agtatgaaac cgaaggagaa atgacagatg aatgaagaaa
     6901 aaaatagggc aatggagaga ctatcaacaa aagatgggat tattagtgca ttagcgatcg
     6961 atcagcgtgg tgctctaaaa aaaatgatca aatcgttggg aaaagagcca agtgaagacc
     7021 aaatcagtga attcaaagaa ctagtatcag ctgagctgac accctatgct tctgctattt
     7081 tattggatcc ggaatatgga ttgccagcag caaaagtcag agcaaaagag acggggttat
     7141 tgttagctta tgagaaaacc ggctatgacg caactacgcc tggtcgtcta cctgatttat
     7201 tagcggattg gtcttctctc agattgaaag aacagcaagc ggatgcagta aaattcttac
     7261 tttactacga tgttgatgaa gcacccgaaa tcaatcattt gaagcatgtg ttcatcgaac
     7321 gtttaggtag tgagtgcgaa ggagaaaatc gtcccttcta tttggagtta gtttcttatg
     7381 atgcacaaat aagcgatagt tcttctccag agtatgcgaa agtcaagccc cacaaagtta
     7441 ttgagatgat gaaagaattt tctgatccaa aatataaagt tgatgtgttg aaagtagaag
     7501 taccagtcaa tatgaattat gtgaatggct attccacagg tgaatttatt cacagcaaag
     7561 aagaggctgc tgccttcttt aaggagcaaa gtgaagcgac tccattacca ttcatttttt
     7621 tgagtggggg tgtttcaaca gaattatttc aacaaacact gatttttgct catgaatcgg
     7681 gttccacctt caatggtgta ttgtgtggac gtgcgacatg gaaagatggg gtcaaagcat
     7741 acgtcgaaaa tggaaaagac ggagctattc attggttaag aacaaatggg agagaaaaca
     7801 tcgagacttt aaataaagta ataaatgaaa ccgctacccc ttggttcaat aaaacaaaaa
     7861 gcaaaaaggt gaagatgaaa tgattacgac agttaccttg aatccttcca tcgatatcgc
     7921 gtatacaatt gaagaattaa acctcgacaa agtcaatcga gttgaaacag tcaataaaac
     7981 agcgggagga aaaggagtga atgtggcgag ggttctaaat ttattaggag aagaggtgat
     8041 agctactggt ttaataggtg gccagcacgg tgaatatcta aagaaccagc tagatcagtt
     8101 agggatcatg catcagtttg ttccaattaa aggagagaca agggattcta ttgctatcct
     8161 gcatagttcg agacaaacag agcttttgga acgaggcccc ttcataacaa agaaagaaca
     8221 acagacattt accgatcatt tcgagcagct cctcgaaata tcagatatta tgacgatctc
     8281 aggtagttta ccccaaggat gccctcttga ttactataag caattggttg ctttagctga
     8341 taaaaaaggg aaagacatat tactagatac ttctggtaaa tttttgcttg aagtattgga
     8401 aggtaaggag aagccactac ttatcaagcc caatctggaa gaattaggag aactgattgg
     8461 ccaacgtatc tgttcttcga ttcaattaga aaaaattcag tccttattag ctcatccaat
     8521 atttgaagga gtgccatggg ttgttgtttc tttaggagag agaggtgctg tggcaaaagt
     8581 tgctcagtgt ttttacgaag tcattattcc taaaatatca gttgttaatc ctgttggttc
     8641 aggtgacgcg acccttgcag gatcggccca tgcgttatca aaaaggcaat caatcgaaga
     8701 gattctcgca ttttctatgg cttgtggtat ggcaaacgcg caacaagaga aaactggctg
     8761 gatcgaccct atgaaagtta cgatgttgat gaaacacatt caagttaaga aaatcaaatg
     8821 aacttaaata taaaagaa
//
LOCUS       DAYYCY010000106         8783 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000106 DAYYCY010000000
VERSION     DAYYCY010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8783
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.106"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1120)
                     /locus_tag="ACHAZB_001998"
     CDS             complement(<1..1120)
                     /locus_tag="ACHAZB_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl-tetrahydropterin synthase-related
                     protein"
                     /protein_id="HFM8614638.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYL"
     gene            complement(1107..2795)
                     /locus_tag="ACHAZB_001999"
     CDS             complement(1107..2795)
                     /locus_tag="ACHAZB_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8614639.1"
                     /translation="MKISKKAFLLVVLILLSTLYSVNFMRNAQEIYTTGDLSFHLSRI
                     KGLSSIFEGPINYTTFNNYGDGLNYFYPFLTIIPAVVFYGISNNLILSYVLYIWLLNI
                     CTILISFYYGEKFFKKIDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVLYY
                     AYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKFV
                     VVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNNDI
                     TTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPISLL
                     QFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVI
                     QSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKA
                     ADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIE
                     ITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
     gene            2941..4578
                     /locus_tag="ACHAZB_002000"
     CDS             2941..4578
                     /locus_tag="ACHAZB_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8614640.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSKGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVW
                     RLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLT
                     LIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIEL
                     DNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTK
                     LAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(4723..5463)
                     /gene="truA"
                     /locus_tag="ACHAZB_002001"
     CDS             complement(4723..5463)
                     /gene="truA"
                     /locus_tag="ACHAZB_002001"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFM8614641.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(5654..6289)
                     /locus_tag="ACHAZB_002002"
     CDS             complement(5654..6289)
                     /locus_tag="ACHAZB_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFM8614642.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIARLSILHLKQ"
     gene            complement(6436..7656)
                     /gene="sstT"
                     /locus_tag="ACHAZB_002003"
     CDS             complement(6436..7656)
                     /gene="sstT"
                     /locus_tag="ACHAZB_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFM8614643.1"
                     /translation="MSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSCLKAIAPI
                     LVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPITLQETVSE
                     KAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSESTKELVDQI
                     SIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLFVALVVYP
                     FLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKESYSISIP
                     LGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGVAGGSLLL
                     IPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELGARKRSGE
                     KIKLKDCLDGLSER"
     gene            7875..>8783
                     /locus_tag="ACHAZB_002004"
     CDS             7875..>8783
                     /locus_tag="ACHAZB_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8614644.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPL"
BASE COUNT         2929 a         1577 c         1306 g         2971 t
ORIGIN      
        1 ataaataaaa tagttctctt gtcttattgt atcttctatt tttgaaaaat atcatcgtta
       61 atatcagaga agagaatatt atttgcaagc ttaagatccg ccaaggaaat tgtaacaatt
      121 tggctggcgt attttgcaga agataccatg gcaccaaact agttgtagct agccaagtca
      181 aacacgtacc ataataaatc tttttttcta tgtttgttaa ttttttaaat aaaatcaggg
      241 gaatacataa agataacaga ccaactagtc ccattgagta agtagttaag tcgttgttca
      301 tagcttcagt cagactatcg aacacattag atgcatgagc atataaattt gtcacgctag
      361 gtttgttgat tttttggtag agcatttgtt caaacatcgg ataccaaaaa gcactcgtca
      421 gaaccaatgt cattcctgcc gcactaaata attttttaaa tatagcaata aaatctgcat
      481 tcttgaaacc tttagaaacc aaacgaataa aaataaaaat gataattaat gctgtactca
      541 ttaatgttga tagtacatgg ctgtacacta gcaaactcat actcaatgct agctttacag
      601 acggatactt ttcttctttt ccagagatga tttgataagc ataaaataat acagggataa
      661 caagtgttat tgcgatagct tcggcaattg cggaacgata gtagatgtct actgtccgat
      721 aagcagagaa aatatagaga caactaaata aaaatgccgc ttttttttgt tttaaaaacc
      781 gctcgccata atgataagct acgagtgttg tacacaagtt caataaccaa acgtaaataa
      841 tatagcttac aatcaaattt tttgtcatcc aatataacat tacagctgga aaaaaagtca
      901 gaaagggata aaaataattt acaccatatc cagtataatt gaatgtctca taattaatcg
      961 gactggtaaa aatagtcgat aaccccttga ttcgagataa gtgaaaagaa agatctccag
     1021 tggtataaat actctgtgta tttttgaaaa agatgaaaga atatatacta ctaactataa
     1081 ttaatccact caatattact acttttttat ttattttcat tcttcttgtt tatctccttt
     1141 ttagtcacat atcgagaatc tttcaactta ttaggtgcaa atcgtttaaa acttgaatac
     1201 accactaaac aataagtcag aatactgatc cataaagcca tgagagcagt tggagtatat
     1261 tttgttgtaa tttctatctt attttctcca gcatttacat ccacattcac taagccatgt
     1321 tttttataag agtcatttac gactttaccg tttactttta cctgtgtacc taaataagca
     1381 tatactggta ctgtcacttt ttgctttttg ttgcttgcca ctgtgtaagt aatgttatta
     1441 tctgcagctt tatacttccc tttttcccac gattctccta cttttacttc atgtttttct
     1501 aagtgaggcc tttcttttat cgcttctgtt ggggcgtaat cgtataatcc tcccatatct
     1561 gatgttgtat aacgtgggag tgtttcttca ttgataacaa tatgaccgtg ttttgattgg
     1621 ataacttttg cgtaattttc ttttgctgct atagacgtga cgagtagaaa caccacagta
     1681 gcgccaatga ttaaccatga atatttttta ttcatagtct ttttcataaa aacaagcgtt
     1741 atgatcattg aagaaaaaaa gatttggata cctaagatac gccaaggaaa ctggagtaag
     1801 cttatcggtg ttttttgaaa caaaaaccat ggaaataaat tagttacgca tacccatgta
     1861 gaaactacgc caacatagat gattttttct tttttctcca tttctttcag tatgaataaa
     1921 ggaaaaatca acgcaaccaa tcctaataac cccatagtgt atgttgtaat atcgttattt
     1981 aacgcttgaa tgatactatc tgaaatagaa agtgcttcta gctgcaaatc tgttttgaag
     2041 ggactgtgta tttcttgata attcatctgc tgcaacatag gataccaaaa atagctagtt
     2101 aatgcacaag tcaaaaaaac agcctttact aaattcacga ccacaaattt tatatcgttt
     2161 tttatttgct tgcttaaaaa caaacgaact ataatcatac aagcaatcaa tgcactacac
     2221 aatagtgtag ttaagacatg agtatacaca agtagactta tactgacaga taaagcgatg
     2281 actttttttt ctctattgaa tatcaatcca taagcataat ataaaacagc tgggataatc
     2341 gtaagagcga ttgcttctgc aattgcggat cgataataga tatcaactgt tcgataagtt
     2401 gcaaaagtat ataaacaact aaataaaaaa gcagcatcta tctttttaaa aaacttttct
     2461 ccataataaa aactaatcag aatcgtacaa atatttaata accaaatata tagaacataa
     2521 cttaatatta agttatttga aattccataa aaaacgactg caggaattat tgtaagaaaa
     2581 ggataaaaat agttcagtcc atctccataa ttgttaaatg ttgtatagtt gattggccct
     2641 tcaaaaatag aagataaccc tttgattcgt gaaagatgaa aagataaatc acctgttgta
     2701 taaatttctt gagcatttct cataaaattt actgaatata aagtactaag taagataaga
     2761 actactaaaa gaaatgcttt tttacttatt ttcatagatt tgttgttctc cttttttaat
     2821 tctattttca gtatactaga attgtctttt tttaataagt tttgcgttaa atttttcata
     2881 gcactagtat gatatactag aaaagaatca atttttatta cttttaaata ggagaaaaaa
     2941 atgaaaaaaa acaaaaacca aataatagat atcattttta tgttatttct aagttttgct
     3001 tacgtcatcc ctctattcat gtctaaggga atctatcata gtgtcaatca ggatacttac
     3061 tttcatttat ctcgaatcat aggactagat aatgtatgga gttctccagt aaacttcaat
     3121 aacttcgatc atcatggaac aatgatgaat attttttatc catggcttac tctttatcca
     3181 gcttttttat tttataaaat gatgggaaat cttgttttaa gttataatat ctactatttt
     3241 tttatcactt ttttaacaat ggtcgtttct tatttttcta tgaaacaaat aaaaaacaat
     3301 cgttatattt cgctgttatt ttcgattatt tatacatttt ccgcctatcg tgccattgac
     3361 atattccatc gtgcttcatt gggagaagct gtcgctttaa catttttgcc tttgatacta
     3421 atggggtgtt acgaaattta tattagagat taccaaaaat ggtactggtt gtctattggc
     3481 atgacgctag tagtgtatac tcatttactt tctgtcgcta tggtttctgt atttattgga
     3541 ggaacgttgt ttctttcttt ttatttttgg gaccaaaaaa tcgctagact cttaagtctt
     3601 ttaaaagcta ccgtcctcac ctttttttta agtgcgggat tcttaattcc ttttattcaa
     3661 caatctagag cacaagaatt gaaagtccca ctgggaaaag aattaagtgg tatggctcct
     3721 agtgatatgc tgacacatat tttaaataat aactataaca actatactat tggattattc
     3781 ttatttctcg gattgattgg tgcttttatt tttataaaaa aattaactgc tgatgattta
     3841 tttattttct ttcttggtgt atttgttctt ttttgttcaa ccaatttatt tccttggcaa
     3901 ctttttaacc atacaccagt taagagtcta caatttgtct ggcggttaaa tggtttttct
     3961 tctttattta tagcctatac gatgagtata gtgatctatt atattttttc tactaaaaat
     4021 aattcatgga aaataatggt ctttacgttt cttgcactaa tattgcatct ctcaggagta
     4081 agcaattcta tccatgctaa caaagattca ttaacactta tagcacctga agacgctgtt
     4141 gctatagctg aaaattacaa tcatactgat tatgcaaaca aagagtcaat ctatcatcct
     4201 gatatgatta ataatgatca atatttacta ggtaaccagg aaataaatcc aacaactcat
     4261 tttatgagta acaaacttac aattgaatta gataattcga ataataaaaa tactgtcttg
     4321 acgacaccta tttatcgtta taaagggcaa gtggcttcta ttaatggcaa actagtacaa
     4381 acaaaattat ctaagtttgg cacaactgaa ttaactattc ctccaggtat aaataaagtc
     4441 gttattactt atcagtacac aaaactagca attgcttcaa gatatttatc tatcgttact
     4501 ttgattttat ttttacttta tcgatttact ttttctaaac aaaaacaacc gagaagagaa
     4561 gtacaacatt ctcactagat ttttatttaa gaaagacttt ttagctgtac acataaacag
     4621 gctaaaaagt ctttttttat atttctttaa tttgcttcat acattattta acatcgatat
     4681 ttatactgtg aaacatgcgt ttcatccatt atcaaagtat ttttatcgat aataaacttc
     4741 atccaaaaac agaccttgtg cagggacggt ttctcctgca tgttgccgca ctttgctttc
     4801 aaatatttca tcgattgttt ctatcggcat ggcacctgtg ccgatttcta gtaatgtccc
     4861 catgataatc cgcaccattt tatataagaa tccgtctccg acaaaagtaa aatgaagcat
     4921 ttctcccttt cgttcgatag ataattcatc aatcgtacgc actgtggatt tttttgattt
     4981 tttcaatgca gaaaaaccaa tgaaatcatg tgtacccact aatttttcgc aagctgcatt
     5041 cagcttgtcc atatccaact cttgagggaa ataaaagctg tggtttcgct caaatgctga
     5101 aggaatcacg ttgttccaaa cataatagct gtattttttg cccaccgcat tatatcttga
     5161 atggaagcgc tcaggcattt cttcgattct tttgatgaca atatcccgcg gaaggtaacg
     5221 attcataaag tcgatcattt ctgccagtcc aatttctgtg tttgttttaa aatttgctac
     5281 ttgtcctcgc gcatgggtgc cagcatctgt tcttcctgat ccaataattt ctatctcttc
     5341 atttgtcatc tgatgcaata ctttttcgat ttttccttga atggttttat cgtcatctcc
     5401 aagtctttgc catcctgaat atctttttcc gtcatattcg attgtcaact tgatgtttct
     5461 catattttag tggctcctct gtcttccatt tggtcttctc tctacctttt taagattgta
     5521 gcacaaaata tagctccttg cttctctaag acagaagcta tacaagaaaa ctgtggaaat
     5581 atatatttca gaaaaacaaa aaacagccac cggatgaagt atccgctgac tgttcaattt
     5641 gacggctcaa gttttactgt ttgagatgca agatactcaa cctggcaatg ggcacataat
     5701 taaagaaaaa actgattctt tggcaaaaac ctattttatt cagtaatggc caagaattta
     5761 tagaaggaat tcttgccagt ccataaatcg ccgcaaaaag caaactgatc acagacagtc
     5821 caaggtataa gtggcttacg tccatttgcc cattttttcg atagatcaag aaaagaagaa
     5881 ctggaaacaa taaaaaacca ctgtaaccta aacctgatcc gatattatgg acccatgtcg
     5941 agacattcca cgaagattca tgggtattga tactgaacaa acctgtaaag atacaatcac
     6001 ctataccata gaacgcgatg gctgctgtaa ctagggtagc taacacacga gatgtactaa
     6061 caaatgcagc atacaatgca ggcaatgaca ggatgaaaaa gatccctgaa accacagacc
     6121 aaataagaaa cgcttttctt acaggactgg aaacctctcc aaatacactg atcacgctct
     6181 tcatttgatc taattcagga taatacaatc ctaatacgta cggagtcaaa aaatcactca
     6241 gcacgcctaa taataaaaag aaaaaaccaa atttacgtaa aaaatccatg atacgcctcc
     6301 aataacaatt acctatgctt ttaattgtta ttataaacga ctttatcttg atttaacaga
     6361 aacctctatt tacacgaaca tctatataac tcattaaaaa ctcgaatcat tttaaaaata
     6421 ctaaatatct aacgttcaac gttcacttaa tccatctaaa caatctttca atttgatttt
     6481 ttccccactt cttttacgcg cgcctagctc tcctattgca gtaaacaaca gatcacttga
     6541 cgaatttaag gcagtttcca ttgaatcctg gataacaccg ataataaatc caataccgac
     6601 gatttgcatc gcgacatcat tagagatacc aaacaaacta catgctaatg gaatcaataa
     6661 cagtgaaccg cccgcaacac ctgaagctcc acaagcagct aaagcagaca aaatacataa
     6721 aaggactttc aaaacaaaag gtgcttcaat ccctaaagaa tgtacagcag tcaacgtcat
     6781 caaggaaatg gtaatcgcgg caccgcccat attgatcgta gcacctaacg ggatcgatat
     6841 ggaataagac tcttttgtca atttcaatct ttcagccaac atcatgttta tcgggatatt
     6901 tgcggcagaa cttcgggtaa agaaagcggg aagtgcgctt tctttcaaac aaaagaaaat
     6961 caatggatag ggattttctt taataaacaa aaacgtgagg aatgggtaca caactaatgc
     7021 gacaaacagc atcgtaccga tcaaaacaag taaaagctgc agatactctg ccaatccagc
     7081 gattccagtc gtagcaattg attgataaac caatcctaaa atccctatcg gagcaaatgc
     7141 aataatcact tgtacgactt gtgacaacgc tatagatatc tgatccacca actcttttgt
     7201 cgattctgaa cgtgctcgaa aagctagtcc caacaataca gcccagaata agacgccaag
     7261 gtaattcgca tcaataagag attgaacagg attttgtgtg acattcgtca acatagtaga
     7321 taaaacactt tccaaacttt gcggtgcttt ttcagatact gtttcttgta aagtaatagg
     7381 aacagtaaaa attgaactta caataactgc tacaagtgca gaaagcaatg ttcccactaa
     7441 atataagatc aaaataggct tcacgaatgt cttattccca attttatgct tggcaattga
     7501 cgccatcgtc aagaaaaaaa ccagaatagg tgcgattgcc ttcaagcaac tgacaaacaa
     7561 cgttcccaat attgaaataa atgtccatgt ggggaaaaag ataccaaaca atattccgac
     7621 taaaatacca acaacaatac gttgaactaa cgacatatcc aaaaacttct ctaacaaatg
     7681 accacccttt cacaatttta acaatcattg tatcatttta tgcaaagaaa ctaaaggtat
     7741 aattttctaa aaattctaac aatacaaaca accatttctt gctaattttg tactttttct
     7801 gtctacacgt tgttttttga gatattctaa gttaggatgt tattaatggg caacttatca
     7861 ggaaacgttt tcttatgttt ttgtccaatt tttttgtgag gtgtttattc ttgaaaaaaa
     7921 taaagaagga gagttatggc ttcctgacaa tctttgtcgc ttgtataatg atggttttac
     7981 agcgtatatt gatttcagca gtcactattc ctacatatgc aacaaaccct ggtcgattct
     8041 acatctatac atttttacaa acttccgttg ttattggtgc aaatttgatc ccactttaca
     8101 tgggttacca atatcaaaaa atcgcccgtt taaaagtttt acattattta agtcgttttg
     8161 cctttatatt cctaatagcc acactagttt gcaatatctt tttttacgta caagctggca
     8221 gcttgaatat tcgtgattat tggcttatcc taacacctat cagtcagaat tactttactt
     8281 atgctgtttc ttgtactttg cttttttgta gtattcctta tacagttgga tttttaaata
     8341 aattatcgaa aacaacaatt aaatatctac tattattttt aacaattgga ttagttggct
     8401 gctctactct atttaataaa gacccgtttg cattacagaa tggacagagt attttatgga
     8461 ttttttattt atttttaatg ggttatggtt tgaaagaatg gaactggaaa gaaaaggtcc
     8521 gctataagct tcctcaagta tttatcagct tgattgtttt attcggtttg attattttga
     8581 tgacacaaat ttcactcatc atcagaggaa atacagatac agcgctgagg ttttctaaac
     8641 catattctct cttcgcaatg tattatactt tctcttcgtt tttattttta gaaatgctat
     8701 ctgataaatt aggtataaaa ggaagaggaa attttcttgc tgtatttttg attgtcactc
     8761 aagttatatg taatttccct tta
//
LOCUS       DAYYCY010000107         8675 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000107 DAYYCY010000000
VERSION     DAYYCY010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8675
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.107"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1101
                     /locus_tag="ACHAZB_002005"
     CDS             <1..1101
                     /locus_tag="ACHAZB_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFM8614645.1"
                     /translation="KKETIVRFEQYNHMPLVTRLKKQRYTCKNCRTHWTAQSYFVRLS
                     HSIADHVRYKIASLLTEKVSLSFIAKSCQVSLTTVTRTLKEFKSYLPKPSKKILPRVL
                     MVDEFRSHASIEDKMSFICADGETGNLIAVLPTRKLPRLTSYFLGCTNPEEVEFLVTD
                     MNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQALNELRIREMNELRKAGQKSQAEKLKK
                     NWRFLLKNRASINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSAPLKEAYPFFHELV
                     EAFRDKDPDLFFSLLAELPETLDDGFREKLQNLLTYEEGITSAMIYPYSNGKIEAKNT
                     HIKTMKRVSYGFKSFENMRIRIFLINQLINVR"
     gene            complement(1280..1615)
                     /locus_tag="ACHAZB_002006"
     CDS             complement(1280..1615)
                     /locus_tag="ACHAZB_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFM8614646.1"
                     /translation="MKMNTNDIATVYVAFTAAEGGKRRPVYILKNGLDTISFLSITSK
                     YHTKSDKIKLKYVEIRDWKQAGLKKESWIDVGTLNELPKNNKKVKWKKIGELSPSDLQ
                     RLEERMEEL"
     gene            complement(1599..1862)
                     /locus_tag="ACHAZB_002007"
     CDS             complement(1599..1862)
                     /locus_tag="ACHAZB_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFM8614647.1"
                     /translation="MAKLQISMDEKLKNEAESIIDELGMSPKTAVTVFYKQIVEQGKI
                     PFSIELSEKSKRIHNIQRLAEKLPVQKLDTDEKIEEWFNEDEY"
     gene            complement(2322..3125)
                     /locus_tag="ACHAZB_002008"
     CDS             complement(2322..3125)
                     /locus_tag="ACHAZB_002008"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM8614648.1"
                     /translation="MRFLHLSDIHFRENYQTNLFTAELFPTIKNPQLALEKLLQELSL
                     TVYDFILLTGDLVHEGGISDYQVLRKMLDQYFPTTPYYFCRGNHDRRSVFFEGMSVES
                     NETGEYIHCTNQNGLRIISLDSAQDTQHEGKISIGQMIQLQEWLGTPAEKGTILLVHH
                     PLSWEDPSIVTEVPEGFAELIAESDILGIFVGHIHQGSSAEFAGKKQFMAESLSFGVD
                     EYPDYSLFTNRTGFTSGSLIDGKIFTYQHYLTPHQIEIGRMKKPITGNH"
     gene            complement(3103..4227)
                     /locus_tag="ACHAZB_002009"
     CDS             complement(3103..4227)
                     /locus_tag="ACHAZB_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8614649.1"
                     /translation="MGETLELRNIGKKYGEHTILKEIDLAVAAGERIVLLGPSGSGKS
                     TLLRMIAGLEKITSGELFLGTQLANKLPCGEREIAMVFQNYALYPHMTVKENIIFALR
                     ANKIKTADIQHRLEEALKMLGLTPYQDRLPKDLSGGQRQRVALARAVVKRSNYFLLDE
                     PLSNLDVRLRLDARKELVKIHQRYKQTFVYVTHDQIEAMTLADRIVLLNEGRIQMIDT
                     PKNVYTKPKNTFTANFIGSPGMNIMPVTYDQGTLKVASQFLTLNPAWQKQLLQVSGSL
                     YLGIRPEHVTFSKETSDLHGQVKYRELLGQSYAYTVKVDGIEMIVLNEKPTWQIGDAV
                     NLRIEPERMHFFDYQTQENLGYPDYFIEERGNSHALFASL"
     gene            complement(4243..5058)
                     /locus_tag="ACHAZB_002010"
     CDS             complement(4243..5058)
                     /locus_tag="ACHAZB_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8614650.1"
                     /translation="MKIKRWLPHLLFMFIIIIIIYPLLFIMANSFKPLKDAYQTITSL
                     IPQPFTLENFCYLFENLPIIKITRNTFFIATIITVVKLGVAFFAAYSFTYCRIKGKKI
                     WYFIIIGTMFIPFTVAMIPNYLLIAKLNLIDTTWGVIIPQFADAMGIFLLTQTMREIP
                     VSLLDVAALDNIKQTTVMSKIVFPLTRHAITSTGIWFFITSWNEYVWPVLILRTVDNY
                     TLPLAMQTYISSEGGTNFTVAMAISLITMFVPLVLYAIFQKYIIGTFVSAGIK"
     gene            complement(5048..5920)
                     /locus_tag="ACHAZB_002011"
     CDS             complement(5048..5920)
                     /locus_tag="ACHAZB_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013669610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8614651.1"
                     /translation="MKRYVGYLYLAPAIILLGVFVFYPLIYTIYLSFFDWNMVAPTKE
                     FVGFANYVKVLTDPVFHKVLKNTLSYTVLFVLLSCVFPYITSFLLGIVIEKGQNLYKP
                     LFFIPAVISLVVAGMVFTWILNPVSGPIAVLLGNFGLRLPMWMNLQGWVIAVIAGITA
                     WKVFGYNFIVIFAAIVGIDQEMIEAAKLDRVPIWRIFFTIVLPMSSSTGLYLLVMTIV
                     QGLQYVFTPIKIITRGGPNNGSANVIYYAYQKAFEMYKVGESSAISVLTLLIFAVFLG
                     VIFKFVEKGVYYEN"
     gene            complement(6005..7294)
                     /locus_tag="ACHAZB_002012"
     CDS             complement(6005..7294)
                     /locus_tag="ACHAZB_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8614652.1"
                     /translation="MLKKNLVIGVVSVLSVISLAACGSGNTKTTKKSEGRVTIEYWHV
                     NADTLGGKTVNELVDKYNQSQDKVKVVAKYNPDMYKGLMQNLQASVASKEIPDVVQVG
                     WAFKDYFAENFKYTNPQSLVEEVDPDNADYFKDHFLDNIMALSEKDGKQVGIPYSISN
                     PVLYLNQDILKAAGLDENGPKTWEEVQTFSKIIKEKTGKYGVYIQEPADNWAQQAILE
                     SNGASVIKDGKATFASKDGIAAYELYQRMVKDQTALHTTWEQGVQSFIDGNVAMVYTT
                     IAQRDNIQKNSTFTAKAVESPSWEGKKVKLPAGGAMLAVTSQTDKKKEATWDFLKYLY
                     SVESLAKWTEGTGYLPPRKDVADAQNGLKAFLAENQMMIPAIDQMDKMVSWSSFPGDA
                     GLEAEQKLLDMRDKILSGNSVNETMKATQKEINELLK"
     gene            complement(7438..7950)
                     /locus_tag="ACHAZB_002013"
     CDS             complement(7438..7950)
                     /locus_tag="ACHAZB_002013"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8614653.1"
                     /translation="MTINCDLKEEGLEMAVFSLAQTYQIWPQIVLSGYVQLSWAAIWG
                     KHIFYNIENQFEGKKLGDITEADLIDTLQKLAYQSVQIVNIHEGWLTKLAREVGQQLG
                     LTFSVWTVNELETIEYLARCGVYNITSRCAWAYLWKKGAQNKLFYNQNRGVKRFGDGI
                     HDHMGALFLA"
     gene            complement(8017..8160)
                     /locus_tag="ACHAZB_002014"
     CDS             complement(8017..8160)
                     /locus_tag="ACHAZB_002014"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFM8614654.1"
                     /translation="MITAHSGSDNTEENSWAFLRFCLDRQLCFEVDVRQQTDGTLILA
                     HDR"
BASE COUNT         2691 a         1850 c         1371 g         2763 t
ORIGIN      
        1 aagaaggaaa cgattgtccg ttttgaacaa tacaatcata tgcctttggt tacgcgccta
       61 aaaaagcaac gctatacctg taaaaactgt cgtacccatt ggacggctca aagttatttt
      121 gtccgactca gccattcaat cgcagatcat gttcgatata aaattgcttc tttactgact
      181 gaaaaagtat ctttgtcttt tattgcgaaa agttgtcagg tatctttgac taccgttact
      241 cgtacattga aagagtttaa aagctattta ccaaagcctt ctaagaagat tctccccaga
      301 gtattgatgg ttgatgaatt tcgttcgcac gcttccatag aagataaaat gagctttatt
      361 tgcgcagatg gtgaaacagg aaacttaatt gctgtcttgc ctacgcgtaa attacctcga
      421 ttaacaagct atttcttagg ctgcaccaat ccagaagaag tagaattctt agtcacagac
      481 atgaacgccg cttacttcca gcttaccaaa cgtgttctgc caaatgcgaa agtagtgatt
      541 gatcggtttc atattgtcaa acacatgaat caggcactca atgagttgcg tatccgtgaa
      601 atgaatgagc ttcgtaaagc aggacagaaa agtcaggcag aaaaactgaa aaagaactgg
      661 cgctttctgc taaaaaatcg tgcaagcatc aaccattatg agtacaaaac atggaaaagt
      721 ttccgagcac caaaataccc atttctcact gaagcaatga tgattgatcg attgcttgag
      781 ttttctgcgc ctctaaagga ggcgtatccc ttttttcatg aattagtcga agcctttcga
      841 gacaaagacc ctgacttatt tttctcctta ttggcagaac ttcccgaaac gttagatgac
      901 ggctttcggg agaagcttca aaaccttctg acctatgaag aaggtatcac cagcgcaatg
      961 atctatcctt attccaatgg aaaaattgaa gcgaagaata cccacataaa gacaatgaaa
     1021 cgagtatcct acggatttaa atcattcgag aacatgagaa ttagaatctt tttgatcaat
     1081 caattaatca atgtaagata acaaaaaatc tgaaccagaa tgtggttcat tctgacccgg
     1141 atttgatttt gcacaactca tcagtcctta ttgacaaaga gccaaaaatc tgagccagaa
     1201 tgaggttcat tctgacccag atttgatttt gtacaaccca tcagtcctta ttgacaaaga
     1261 gcccttcttt ttagtattat tataattctt ccatgcgttc ttccagtcgc tgtaaatcgc
     1321 ttggacttaa ttccccaatt tttttccatt tgaccttttt gttgtttttg ggtaattcgt
     1381 ttagagttcc tacgtcaatc caagattctt tttttagtcc tgcctgtttc caatccctta
     1441 tttcaacgta tttcagcttt attttatcgg atttagtatg atattttgaa gtaattgaca
     1501 gaaacgaaat tgtgtccagt ccatttttta gaatatagac aggccggcgt ttaccgccct
     1561 ctgcagctgt gaaagcaacg tagacggttg ctatatcatt agtattcatc ttcattgaac
     1621 cattcttcaa ttttttcgtc agtgtctagt ttttggacag gcaatttttc cgctaatctt
     1681 tgaatattat ggattcgttt acttttttcc gacaattcaa tagaaaatgg gatttttcct
     1741 tgctcaacaa tttgcttata aaaaacggtg acggctgttt tggggctcat gcctagttca
     1801 tcaataatgc tttctgcttc atttttcaat ttttcgtcca tagaaatttg taacttagcc
     1861 attttctcca cctctttctg acaaacacat tatatcacgg aaagagttca aatggaatta
     1921 atacaactac ttttgaactc cttgaaatgc agtgtattta tctaaatata aggaaaggat
     1981 atagtgctaa atggccttga tagatagaaa agtttcaaaa tccgccacag cgataattga
     2041 agcgaacact caacgacaca cagaacagga aaagactcct aaaagaacca aattaactag
     2101 gacgtgtctg aaaactccac aaagatgtgg tatgctctgg taaaaaacga atggcacaac
     2161 cacgaagacc gatatggttc ttggaaaact ttctccaccc tttttgtaat tggagggata
     2221 gtggtttatt gatagcgatt ttccaagttc ttcaagtgga accagacatt gagcaccacg
     2281 gctaaaaagt gaatgaagtc tttattttta atcaataatt tttaatgatt accagtaatt
     2341 ggttttttca tgcgccctat ttctatttga tgtggagtta aataatgctg ataagtaaaa
     2401 atttttccgt caattaaact gccactagtg aatcctgttc tattggtaaa aagagaatag
     2461 tctgggtatt catctacacc aaatgataaa gattcagcca taaactgttt tttaccagca
     2521 aattcagcac tgctaccttg atggatatgt cctacaaaaa tacctaaaat atcactttcg
     2581 gcaatcaatt cagcaaaacc ttccggaact tcagttacaa tagatggatc ttcccacgac
     2641 aatggatgat gaaccaataa aatcgttccc ttttcagcag gtgtacccaa ccactcttgt
     2701 aattgaatca tctgtcctat cgatattttt ccttcgtgct gggtatcttg agcagaatcc
     2761 aaggaaataa ttcgtaaccc attttgattc gtacaatgga tatactctcc cgtttcattg
     2821 ctttcaacac tcattccttc aaagaaaaca ctgcgacgat catgattccc acgacaaaaa
     2881 tagtaaggtg tggttgggaa atattgatcc aacattttcc gtaagacttg ataatcagaa
     2941 atcccccctt catgtactaa atcacctgtt aatagaataa aatcgtacac tgttaacgag
     3001 agttcttgta acaatttttc caatgccaac tgtggatttt taattgttgg aaatagttca
     3061 gcagtaaaca agttagtttg atagttttct ctaaaatgga tatcagagag atgcaaaaag
     3121 cgcatggcta ttccctcttt cttcaataaa ataatcagga tatcctaaat tttcttgcgt
     3181 ctgataatca aaaaaatgca ttctttcagg ttctatgcgg agattgactg catcaccaat
     3241 ctgccaagta ggtttttcat taaggacaat catttctata ccatcgactt taacggtgta
     3301 cgcataactt tgacctaata gctcacgata cttcacttga ccgtgtaaat cgcttgtttc
     3361 ttttgaaaag gttacgtgtt ctggtcttat gcctaaatac aatgagccac taacctgaag
     3421 aagttgtttt tgccaagctg gattcaatgt taaaaattga ctggctacct ttaatgttcc
     3481 ttgatcataa gtaactggca taatattcat gcctggcgaa ccaataaaat ttgcagtaaa
     3541 agtattttta ggtttcgtat aaacattttt cggcgtatca atcatctgaa ttctaccttc
     3601 attcaataaa acaatccggt cagctaacgt catcgcttca atttgatcat gagtgacgta
     3661 gacaaaagtt tgtttataac gttgatgaat ttttaccaat tcttttcgtg catccaatcg
     3721 taaacgcaca tccaaatttg ataacggttc atctaataaa aaataattac tccgtttaac
     3781 caccgcacga gccaatgcta cacgctgccg ttgccctcct gacaaatctt taggaagtcg
     3841 atcttggtat ggcgttaaac ctaacatctt caaagcttct tccaaacggt gctgaatgtc
     3901 agctgtctta attttattcg cccttaatgc aaaaataata ttctccttta cagtcatgtg
     3961 aggatataaa gcataatttt gaaaaaccat tgcgatctct cgttctccac aaggtagctt
     4021 atttgctagt tgtgtaccta aaaaaagctc tccactcgta attttttcca aaccagcaat
     4081 cattctcagc aaagtagatt ttcctgaacc agatggtccc agtaacacaa ttcgttctcc
     4141 agcggccaca gctaaatcaa tttcttttaa aatggtatgt tctccatatt tttttccgat
     4201 atttcttaat tcaagcgttt ctcccatgat ttgtcctcca atttatttaa tacctgcaga
     4261 aacaaaagtc ccgataatat atttttgaaa aattgcatac aagactaacg gtacaaacat
     4321 tgtaattaaa ctaatcgcca ttgctacggt aaaatttgtc ccaccttcag aactaatata
     4381 ggtctgcatc gccaacggca aagtataatt atctactgtc cgtaaaatta ataccggcca
     4441 aacatactca ttccaagagg taataaaaaa ccaaataccg gtagaagtaa tcgcatgtct
     4501 agtaagagga aaaacaattt tactcatgac ggttgtttgt ttgatgttat ccaatgccgc
     4561 cacatctaga aggctcactg gaatttcccg cattgtttgc gtcaataaaa aaatgcccat
     4621 cgcatcagca aattgtggta ttatcacacc ccaagtagta tcaattaaat taagttttgc
     4681 aatcaacaaa taattaggaa tcatcgcaac agtaaaagga atgaacatcg taccaatgat
     4741 gataaagtac cagattttct tccctttgat tcggcaataa gtaaaactat atgccgcaaa
     4801 aaaagctacg cctaacttaa caaccgttat aattgtagca ataaaaaaag tatttctcgt
     4861 aattttgata attggaagat tttcgaacaa atagcaaaaa ttttccaagg taaacggctg
     4921 tggtatcaaa ctagtaatcg tctgataggc atctttcaat ggcttaaaag aattggccat
     4981 aataaacaat aatggataga taataatgat tatgatgaac ataaataaga gatgtggcaa
     5041 ccagcgttta attttcatag taaaccccct tttccacaaa tttaaatatt acccctaaga
     5101 aaacagcaaa tattaataaa gttaaaactg aaattgctga agattctccg actttataca
     5161 tttcaaaagc cttttgataa gcataataga tcacgtttgc actaccatta ttcggtccac
     5221 cacgagtgat gatcttaatc ggagtaaaca catactgcag accttgaaca atggtcatca
     5281 ccaataaata tagtcctgtc gaactactca taggcaacac aattgtaaaa aaaatgcgcc
     5341 agatgggtac acgatccaac ttagctgctt cgatcatctc ttgatcaatt cccacaattg
     5401 ctgcaaaaat gacaatgaaa ttataaccaa aaactttcca agcagtaata cctgcaatca
     5461 cagcaattac ccaaccttgt aaattcatcc acataggcag tcttaaacca aaatttccca
     5521 acaagacagc aattggtccc gataccggat ttaatatcca tgtaaaaacc atccccgcca
     5581 ctaccaaaga aatcacagct ggaataaaaa ataatggttt atacaaattc tgcccttttt
     5641 ctataactat tcctaataaa aaggatgtaa tatatgggaa cacacagctc agcaagacaa
     5701 atagaacggt ataggacaaa gtattcttca acactttatg aaagactgga tcagtcagca
     5761 cctttacata gttggcaaag ccaacaaatt ctttagtggg ggccaccata ttccagtcaa
     5821 aaaaactcaa atagatcgta taaatcaatg gataaaaaac aaacacgccc aacaaaataa
     5881 ttgctggcgc cagatacaaa taacctacat agcgcttcat ttccgacagc ctcctcatcc
     5941 ttatgtcaaa agcagacgac cttgtttttc ctttcttaca ggcgctgtcg tctgcttgct
     6001 ttgcttattt gagtaactca ttaatttcct tttgagtagc tttcatcgtt tcattgacgg
     6061 aattacctga taaaatttta tcccgcatat ccaacaattt ttgttcagct tctaatccag
     6121 catctccagg aaatgaactc caactgacca tcttatccat ctgatcaatt gctggtatca
     6181 tcatctgatt ttcagctaag aaagctttta atccattttg ggcgtctgca acatctttac
     6241 gtggtggtaa ataaccagta ccttctgtcc atttagctaa agattcaaca ctataaagat
     6301 attttaagaa atcccaagtt gcttcctttt tcttatccgt ttgtgatgta acagctaaca
     6361 ttgcgccacc agcaggtagt ttaacttttt tcccttccca acttggtgac tcaactgctt
     6421 tagcagtaaa agtactattt ttttgaatgt tgtccctttg agcaatagtg gtatagacca
     6481 tcgcgacgtt accatcaata aagctttgca caccttgttc ccacgtagtg tgtaatgccg
     6541 tctggtcttt taccatcctt tgatataatt cataagcagc gataccatct ttagaagcaa
     6601 aggtagcttt gccatctttt attacacttg ccccattact ttctaaaatt gcttgttgtg
     6661 cccaattatc agcaggttct tgaatataca cgccatactt tccagttttt tcttttatta
     6721 tcttagaaaa agtttgaact tcttcccaag tttttggacc attttcatcc aagcccgctg
     6781 cttttaatat atcttgattt aaatataaaa ccggattact aatggagtaa ggaataccca
     6841 cttgtttacc atctttttca gacagtgcca tgatattgtc taaaaaatga tctttaaaat
     6901 aatcagcatt atccggatcc acttcttcta ctaaagattg aggattagta tatttgaaat
     6961 tttctgcaaa ataatcttta aaagcccatc ccacttgtac tacgtccggt atttctttgg
     7021 aggcaacaga agcttgtaaa ttttgcatca agcctttata catatcagga ttatattttg
     7081 ctacaacctt cactttgtct tgagattgat tgtatttatc tactaattca ttcactgttt
     7141 ttccacctag ggtatccgca ttaacatgcc aatactctat cgttacacga ccctccgatt
     7201 tttttgttgt cttggtattc ccactaccac aagctgccaa agagatgaca cttaaaacag
     7261 acacaacccc tataacaaga tttttcttta acataacatt aacttcctcc taatttcttt
     7321 atggccattt ttctaatcaa gaaatattat gaactaccaa tgtaaatttc tagttaattt
     7381 tctgtaaata attcgaaatt tgtgcatttc ggggtacagg gtcactggac atatccatca
     7441 agctaagaat aaagccccca tatggtcatg tatcccatct ccaaatctct taactcctct
     7501 gttttgatta taaaatagtt tattctgtgc tcctttcttc cataaatagg cccatgcaca
     7561 acgtgaggtt atattataaa caccacagcg cgcaaggtac tcaatcgttt ctagttcatt
     7621 gactgtccat acagaaaatg tcaatcctag ctgctgcccc acttcacggg ccaacttagt
     7681 taaccatcct tcatgaatat tgacgatttg aacgctttga taggctaatt tctgtaaggt
     7741 atcgatcaaa tctgcttcgg taatatcccc aagttttttc ccttcgaact gattttcaat
     7801 attataaaat atatgctttc cccaaatcgc agcccaagat aattgtacat agccactaag
     7861 aacaatttgt ggccagattt gataggtttg tgccagcgaa aaaacggcca tttccaatcc
     7921 ctcttctttt aaatcacagt taatggtcat ggttgccttc tcattggcca ctgcttgaaa
     7981 cacctgctca agggttaccg aatctgggac aacttcttac ctgtcatggg ctagtattag
     8041 cgtaccatct gtttgctgac gcacatctac ttcaaaacaa agctggcgat ccaaacaaaa
     8101 gcgaagaaat gcccagctat tctcctccgt attgtcacta ccagagtgtg cagtgatcat
     8161 ggtcattttg acccctcctt taattccgtg attttctccc acacgtccac tataccgatg
     8221 aagcgttaat ttcttgttaa tttcttgtaa attccctgcg ttttttcagt aaactagttg
     8281 taaatcttgc atgctccttc ctacgaactc atgaatcgtc gattttgccc ttctagctag
     8341 tttcaccaat taagtagttc tacattcccc gcttttaacc tactacacct ttttttgttt
     8401 tcagctattc cttcttcttg ctgaaagtcc ttgaggttct ttcttacagg ataccatcaa
     8461 ggtgttcaat ggcttattcc cattgaatga ccgagactta ctatcagatt tctgctcgtt
     8521 gaaccaagtc tttgatacag gatttcttcc gtcatcttct cacaaatacc accttgcgtt
     8581 tctctagcga ttggtcgatg tgcaccctcc cagtggactg tcactacccc tttttgctat
     8641 gcctagccca caaaaaaagc tgtcctcaca gacag
//
LOCUS       DAYYCY010000108         8609 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000108 DAYYCY010000000
VERSION     DAYYCY010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8609
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.108"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            278..787
                     /locus_tag="ACHAZB_002015"
     CDS             278..787
                     /locus_tag="ACHAZB_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM8614655.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            880..2042
                     /locus_tag="ACHAZB_002016"
     CDS             join(880..1156,1156..2042)
                     /locus_tag="ACHAZB_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM8614656.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLIPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2322..3302
                     /locus_tag="ACHAZB_002017"
     CDS             2322..3302
                     /locus_tag="ACHAZB_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8614657.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3424..4842
                     /locus_tag="ACHAZB_002018"
     CDS             3424..4842
                     /locus_tag="ACHAZB_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFM8614658.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4913..6382
                     /locus_tag="ACHAZB_002019"
     CDS             4913..6382
                     /locus_tag="ACHAZB_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8614659.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6392..6859
                     /locus_tag="ACHAZB_002020"
     CDS             6392..6859
                     /locus_tag="ACHAZB_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFM8614660.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6895..7767
                     /gene="scrK"
                     /locus_tag="ACHAZB_002021"
     CDS             6895..7767
                     /gene="scrK"
                     /locus_tag="ACHAZB_002021"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFM8614661.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7848..>8609
                     /locus_tag="ACHAZB_002022"
     CDS             7848..>8609
                     /locus_tag="ACHAZB_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM8614662.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNN"
BASE COUNT         2953 a         1297 c         1739 g         2620 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgtgata aaaaacgaga aatgaattat
       61 aggtttagac ctttatcatt tctcgtttta ttttattgta tcaaggctct atcataagtg
      121 gtaaaatcta cttttaaatc acatgtaatc attgataagt ccagtgtttt agcggataat
      181 cagcttttta tttatttttt cttataaatt tgataaatag aaattagtat agccctaaac
      241 aaattatttg tgttttattg tttagggcta tactatattg gtacaaggaa tagaaagagg
      301 tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca tcaaagtaaa
      361 taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc taaaactcag
      421 tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta gtattacgga
      481 attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc ccactttaga
      541 gaaaatgaat cttataagca tttctcaaaa aaaaggaaat aagaaaacta aacttgtatc
      601 gttaacaaaa tatggtcgaa aggtgtacga atcacataat tatttacatg aagagcttta
      661 tagctcaata aatgaagcgt tagaatcatt ctcaccagac caaatttcaa ccattaaaca
      721 gattctagaa aaagtatctt atacaatcaa tgaatatcaa gtaagtctga caagaaagga
      781 agattgacat gaaaaaaatg atttttgaat cggctacact agactagaca gaaaaaataa
      841 ggtgtgtaaa ataaatgaaa acacactgga ggaattttta tgtcaagacg tcaacgtaga
      901 acctattcaa aagaattcaa acaacaagtc gtcgatcttt atctcgcagg aaagccccga
      961 gctgaaatca ttcgagatta tgagctgact acctcggcct ttgataagtg ggtcaagcaa
     1021 gcgcaaacta ctggctcgtt tcaggaaaaa gataacttga tccctgaaca agtagaacta
     1081 attgcattac gaaagaaaaa taaacagctt gaaatggaga atgacatttt aaagcaagcg
     1141 gcgctgatat tgggacgaaa agacaagtaa ttgataccaa taagcacaaa tactcaatat
     1201 cagcgatgtg taaagtgtta aacatctctt gccaaaccta ttattatcaa gccaaaccag
     1261 ttgaagatga atcagaactg gaagaactcg ttcaagaaga atttattcgc aatcgaaaag
     1321 cttatggtac caggaagctg aagcagtgct tggccaatct aggtcttcaa gtgagtcaac
     1381 gcagaatcgg ccgtatgatg aaacagcgtg gcttgcgttc aacatacaca ttggctcatt
     1441 tcaaattcca ttcttctact tgtaacgaag ccaagaccgc caatattcta gacggaacct
     1501 tcctacagga aaagtcatta gaagcgattg taaccgacct tacctatgtt cgtgttggga
     1561 agaaatggca gtatatctgc ctgattcttg atttgtttaa tcgagaaatc atcggctatt
     1621 catgcgggga gaagaaagat gcgacgttag tcaaggaagc ttttgcaaag attccgtact
     1681 cgttgaataa tgtacaactt tttcacacgg atcgtggaaa agaattcgat aaccagacga
     1741 ttgacgaaat tttatgggcc tttaatatca cgcgttcctt aagcaaaaaa ggctgcccct
     1801 atgataacgc cgttgcagaa tccacctata aatctgtcaa agtagaattc gttcatcaat
     1861 accgctttga gacattggct aatttgcgat tagagctatt tgattacgtc cattggtgga
     1921 ataccatccg gctgcacggc acgttaggct atgagacacc gatcagtttt cgacagcaga
     1981 gattggcgca gcgaatcctt gataatgagc tcggatgtga taccggtggc gaggcacctt
     2041 aattatttga atgagcttct gccttagaga atcagattcg aggaggctcg ttgtcaaggg
     2101 cagtcggaac accagcagca agcttacgca ataccttata atttttgtca aaaaaagtgt
     2161 tgccattcca catatatctt taaacagcta ttgccagata aaaataaatg atattatata
     2221 tataaatact tatccgaaaa ggggaatgag agagtcaagg ttcttggaat gtcgaatagt
     2281 cgagtaggta gattttctat ctacaacctt gttgtttaaa aatgaaaaaa attggaatta
     2341 gagaagttgc gaaagaggca aatgtttctc ctacgactgt gtcgagggta ttgaatgatc
     2401 gtggatattt gagtgaggaa acaaaaagaa aagtttttga tgccatgaaa aaattagaat
     2461 actatcctaa tgaactggcg agagcacttt ttaaaaacga aacatatacc gtaggtttga
     2521 tttttcctac aattactaat ccatttcacg ctgagttaat tcaagatatc gagttcgagc
     2581 tttccagcag gggatataaa gtgttgctgt gcaatagctt aaacaatcca acaaaagaaa
     2641 aagagtactt gacgatgttg agaaagaatc aggttgatgg aattatagta ggaacgcaca
     2701 ataaagatat tgcggattat gatattccag ggctacctat tgtagcgatt gatagaagtc
     2761 ttggtgaaaa tacagttaca gtatcttgtg ataattacag gggtggggaa ttagctactc
     2821 aactacttgt aaatcatggc tgtcagaata tactgtgtat tcggggagat agtaagataa
     2881 agctacccgc gaatagtagg actttagcat ataaagatgt tttgaataaa ttcatgctgg
     2941 aaccgttagt cctagaagta ccatttgtaa aagaaatcaa tgaaaaaaat gaaataatta
     3001 agacatatct ggaaactcat ccaaatattg acggggtttt tgcaggagat gatttattgg
     3061 cttctttggc gattaacaac ttaggaagtt cgggcaaaag agttccaaaa gaggttaaag
     3121 tgattggatt tgatggagct caacaaacat tgatttataa tccagaatta tcaactattc
     3181 aacagcctat agatcaaata tctaaagtag cagtaaaaaa attattgaat atgattaagg
     3241 gcgaaaagga gacggatgaa ctttcacttc cagtaaagct cataaaaaaa aatacagcat
     3301 aagttaaatc ggcgaaaaat tccttcgtcg atttaaaaat atttttatga aacgggttga
     3361 catatgaaac gggttgacat atattagttg tgaagataaa aaccttttca ctggaggaag
     3421 aaaatggaga aattattaat taaaagcaat aatcatcaac tttactacca acctaaggat
     3481 gtttgggtcg gagatgttat gccttacggg aaagaaggaa aattctttat ttatcatcaa
     3541 cgtgatacta gaaatccagt tccttttgga gaaccgtttg gctgggcttt agcaactacg
     3601 aaagattttg ttcattttga agactttggg gaatctcttt taagagggga tgacaatgag
     3661 agagaccagt ttatctacgc tggttctgta tttgaggcaa atggaaaggt tcacgcattt
     3721 tatacagggt ataatcgtga attcttagcg cagggaaaga catctcaagt gttgcttcat
     3781 gctgagagtg atgattttgt tcattgggaa aaatcagaaa cagcattgga gttagaacca
     3841 caagaagggt atgatattcg aaactggaga gatccatttg tcgtttggga tgatgaaaaa
     3901 gaagaatatt tgctaatttt aggggcgcga aagggtcagg ataaacatct acaaaccgga
     3961 cgtttagtgg aatttacatc aaaagattta actaattggg aattcaaagg tgatttttgg
     4021 gctccgggat tgtataccat gtttgaaatg ccagaacttt tcaagattgg tgactggtgg
     4081 tatcttgtct attcagaata ctcagatgga aataaaatac tctatcgaat gagtcaaagt
     4141 ttggaaggtc cttggattaa gcctaaagat gaagcttttg atggacgggc ttattatgca
     4201 ggtagaacag cttatgatgg aaaacgtcga gtcctatttg gatgggtacc aacaaaagat
     4261 caagaaaatg ataaaaacaa ttatttatgg ggaggtactt tggttcctca tgaagtctat
     4321 caaaaagagg atttttcact tggggtaaaa cccatagacg aagtgtggaa tgcattcata
     4381 aatttacatg atattgccga tgtagagata gaaacagcag acacaagaga agaagtaacg
     4441 cttgtcaaaa atagtggaac tttgtttaat ttagaaacaa caatttcatt tgatgagaat
     4501 cttagtgact tttcactgag attatataaa aaccttgaga cagatgaatc ttacgaatat
     4561 cgcttcaatt tagatgaggg gcgtcttaca tttgataaaa acccttgtta cccttggttt
     4621 caatatatgg ataaaggact tattagacct atatctttaa agcctaacca atcttataaa
     4681 ctaaaagtga ttgttgatga caatataatg acactttata ttgatggaat cgctcttaac
     4741 gctcgtgtac atggttgttt tggtcaagca attactatta cagcaacaaa tggatcaatg
     4801 actgcaataa ataccaagtt ttcaaatgaa ataaagaaat aataaacgtc aatcatgtgg
     4861 caggtctgaa aatcaaacat gacctgtcac atttaaaagg gaggataaat gaatggacta
     4921 caaaaaaatt gccgaagatg tattggaaaa cgttggagga aaagagaata tctcaagcgc
     4981 tacacactgc attactcgtt tacgtttaat tcttaaagac aaaagcaaag cgaatatgga
     5041 aatgcttgag gaaatagaag gtgcaaaagg tgttcttttc aatagtggac agttacaagt
     5101 aattttcgga acaggtataa ttgaaaaggt ttacaaagag ttcgttgaat tgacaggagc
     5161 taaggaagta tccgtttctg aaatgaaaga cgaaggagtc aataattcag gaaatagact
     5221 tcaacgagct tttaaagttt tctcggatat ctttattccg atcattccag cttttattgg
     5281 ggcagcaatg gttttaggaa ttcgttcgtt gattaccact cctggaatgt ttggaatgga
     5341 tggctcattc gctgatcaca atgtattcct aaatgattta tccaaatttc tgggtattat
     5401 tgcaaccaca tttgattatt taccagtttt agttatgtat tctgcaacaa aacgttttgg
     5461 cggaaaccct attttaggat tacttgtagg atttgttatg attcatccag gactagccaa
     5521 tcggaatgat ttcgtcatgg gaactataaa gcctgattat tggcatttat ttggattaag
     5581 cattccagca gttgcgtttc aagggggtgt ttttccagca attttgactt cttggttttt
     5641 agcaaaagtt gagaaaatta caaataaata ttctcctcaa gtacttgctt acattttagt
     5701 cccaactgtg acaattgttc ttgccaactt tgcactattt ttagtgtttg gtccattggg
     5761 gaattttatc ggtaatgttt taggtggaat cattgatttc ctatatatga agatgggtgc
     5821 ctttggtgct tttgtttttg caggcgcgtt acaaccatta gttgtaacag gaacacaaca
     5881 tgctattcaa ggtattgagg caaacttagt cgcaacaact ggattcaatt atatccaacc
     5941 tatttggtca gtttcaatta ttgcccaagg tggcggttgt attggtatgt atttactctt
     6001 taagaaaaaa tctaaagata gagatattgc gatttcaagc tttattccaa cactggtagg
     6061 tattactgaa ccggctatct ttgctgttaa tttgaaatat tcaatcgttc cttttgtttg
     6121 ctcgtttatt ggtgctggtt ttggtggagc atttatgaaa atttttgatg taaaagccat
     6181 tggacaagga ttgactgtat tgccaggtct gactattgct aatccagttc ttccttatat
     6241 atttggtaat ctcatagcat tcattttacc aatcattttt attctcgctt atgataaata
     6301 taaagtgttt atccctgaag ctaaacaccg taaatttgca gaaaaaactg actcaaaaaa
     6361 attgccagtt caagtaaagt gagatgagag catgttaaat ttttttagaa aaaatgaaat
     6421 aagcgttgta gcacctgcaa ccggtgtttt tgaaaaactg gagaaattaa gtgatccagt
     6481 cttttcaaaa ggaatgatgg gacaaggatt tgcaattgat gcaaaaaacg aaacgattgt
     6541 atctccaatc gatggttttg tttcaagtat ttttcctaca aaacatgcct taggaataaa
     6601 aacaaaatca ggaattgaga tacttgttca tgtaggaata gatacagttg agctaaatgg
     6661 tgagggattt gatattaaag ttcaagaagg acaaaaagtt aaggctgggg atattcttat
     6721 gaccattaat tttgtagttt ttgaaaagaa tcacaagact aaagatgtca ttgttgtttt
     6781 tccagactac caagacgaaa ttttcattcc gaaacttgaa gaagtaaaac aaggacaaga
     6841 attgtttgtt cttggctaat gataagtaat taataataga atcgaggaaa atgtatggtt
     6901 tttggggcta ttgaagctgg tggaacaaaa tttgtttgtg ccattggaga taaagatttg
     6961 aacatatttg aaaaagtcag ctttcccacg actacaccca aagaaacaat gaatttagtt
     7021 ttagattttt ttaatcatca taaagatgat ttagaaggta tcggagtggg atcgttcggt
     7081 cctattgata ttcattgtga atctaaaacg tatggcttca ttacatcaac gccaaaactt
     7141 gcttggcagg attttgattt tattggaagt ttaaaaaagc aacttaatat tccaactgct
     7201 tggacgactg atgtaaatgc ctcttgctat ggtgaatatg tttctggaaa tggcagaggc
     7261 ctttcaagtg ttgtttacta taccgtagga acgggcatag gaggtggcgc tatccaaaac
     7321 ggaaacttca ttgaaggttt tagtcatcca gaaatgggac atatgcttgt aaaacgacac
     7381 aataaagaca acttttctgg gacatgtcca ttccacggag actgtttaga gggcttagct
     7441 gctggtccgg caatcgaaag aagatcaggg aaaaagggac aagaagtgac tgaaaatgac
     7501 gttacttggg aaatagaagc ttattacatt gctcagtgtg catataatac aacattgatg
     7561 ttatcacctg atgtcattat atttggtggc ggagtaatga aacaaaaaca cttaaaaaag
     7621 aaagtacaag ataagtttat tgagttgatc aacggatatg tcgctattcc cgatgttgac
     7681 aattatatta tgacaccaaa attgttagat aattctggta ttgtaggttg tttagcttta
     7741 gcaagagacg ttaaagaaaa atcttaattt ggaaaaaaga tcgtctcgac tatttgtttg
     7801 gttgatgcga tctttttttc aattagtaat tgaagggacg cctaaaagtg aaaaaaaaaa
     7861 tgaaagatga agtattagag tatatcaaaa ataatttaaa gtattacgat ttttctgctc
     7921 aagatattgc aatgaaattt tgtattaaaa gaaatgtcgc ttcccactat ttgaatcagt
     7981 tattttcaga tggaaaatta ctaaagaatg attctgttag accagtaatg ttcaagtata
     8041 atcaacaaaa gccaaaagac tgcttttcta agtttattgg agcagacata tctttgaaat
     8101 ccactataga taaatgcaaa gctactgtaa tgtatccccc taatggcctc ccattaataa
     8161 taaaaggaaa cagtggcgtt ggaaaaagtt ttttagcaag tttgatctat cagtatgctc
     8221 ttgatagaaa agtaattcat aatgacgcaa aatttgttgt tgtgaattgt gcagattatg
     8281 caaataatcc agagctattg tcagcagttt tatttggata taaaaaagga gcctttactg
     8341 gggctgaaaa agatactgca gggatgctat cttctgctaa tggaggatac ctttttttag
     8401 atgaggttca caatctaagt gcagaaaacc aagagaaatt gtttcttctt atggattctc
     8461 aaaagtatcg taaacttggt gaaagtgtgc aatgggagta tgcaaatgtt cgccttatct
     8521 tagccacaac agaagataaa aatacctctt tgttagcaac ttttagaaga cgcatcccag
     8581 ctgaaatcac tctgccagat tacaataat
//
LOCUS       DAYYCY010000109         8574 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000109 DAYYCY010000000
VERSION     DAYYCY010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8574
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.109"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..778
                     /gene="menA"
                     /locus_tag="ACHAZB_002023"
     CDS             <1..778
                     /gene="menA"
                     /locus_tag="ACHAZB_002023"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFM8614663.1"
                     /translation="NYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFVI
                     GVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVYV
                     NTFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRDI
                     ENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKHL
                     DQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(1592..2938)
                     /gene="gor"
                     /locus_tag="ACHAZB_002024"
     CDS             complement(1592..2938)
                     /gene="gor"
                     /locus_tag="ACHAZB_002024"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFM8614664.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(3072..4325)
                     /locus_tag="ACHAZB_002025"
     CDS             complement(3072..4325)
                     /locus_tag="ACHAZB_002025"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1901576 - obsolete organic substance
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFM8614665.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(4331..5254)
                     /locus_tag="ACHAZB_002026"
     CDS             complement(4331..5254)
                     /locus_tag="ACHAZB_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFM8614666.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(5475..7967)
                     /locus_tag="ACHAZB_002027"
     CDS             complement(5475..7967)
                     /locus_tag="ACHAZB_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HFM8614667.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(7974..8441)
                     /locus_tag="ACHAZB_002028"
     CDS             complement(7974..8441)
                     /locus_tag="ACHAZB_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HFM8614668.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT         2355 a         1814 c         1422 g         2983 t
ORIGIN      
        1 taattacatg gattatgaaa aggctcattc tgaagtatat aagtatgaag aaaatgtcat
       61 tggccgcaga aatctatctc ctgtattgat tcgaaacatg attttagcta tgattgcatt
      121 tgtttttgtt atcggcgtta ttctaacgat caatacgggt tggcttttac ttttgatggg
      181 atttatctgt tgttttattg gagtattcta tacattcgga ccgattcctt tatctcgaat
      241 gcctttaggg gaaattttca gtggggtgac tatgggcctt ggaatttttg ccatggtcgt
      301 ttatgtcaat acattcggtt cagatgtcta tgatctgaca gtcaactttt catctggaca
      361 atttgatttg gtaggaaatc tttgggctat agcagcaatc gtattagctt ccttaccctt
      421 ggtgtttacg attgccaata tcatgttggc taataatttg cgggatcttg accgtgatat
      481 tgaaaatcat cgctatacac tagtttttta tatcggacgt cctgcgggaa cagtcttatt
      541 ccagctattg atgtatgctt gctatttcgt gattttaatg gggttgttga gcggtatcta
      601 tgaatggccg atcttggtca ccttcttgac cttgccaatt gtgaagaagc atttggatca
      661 attcaagcaa acgttacccc agcctatcag tttcagctat tccatcaaaa atatggtctt
      721 attcaatgcc agttatgtat tggggctatt actaacaatc attatgcaag ccatttaaca
      781 gcatgaagag gttgtgaaaa aactgccctg catcaagtaa taagaacagc caaataaaaa
      841 tagctcctta tatttttcgt ttggttgggc ttattaccgc agatgcaagc ttttgaacac
      901 cgtttatcag gaggttgtga aaaagtcgct tagtttcgag tattaagaaa aattttgtga
      961 aaatggcttt ccacattttt gcataaattt ggcttaatac cgagaaactg gcttttgaac
     1021 accgtttatc agaggttgta aaatcagtgt tttgtcctga gtattagaga aacaaatgga
     1081 aaaatagttc ctcatatttt gccgtttttt gagctaatac cgaaggacag ctgattgaac
     1141 actatttgtt cggaaaaagt gactaactat gacacaattt ttttcacagt cccttttttc
     1201 acttgatttg attcgtacca gcgaacttga cgagaacttt cttggtttgg taactatatg
     1261 gtattctttt aacatcctcg tttgcttctt ctaatttatt tatacaaagt cacacttttt
     1321 taaacatctg ttcttcgata catttctatt tcttttaaac atcgatacca atattttgaa
     1381 cttaagagtg cataccaaat ggattcattt actcttcgtc agttacgaag ataatcaaaa
     1441 tctccaactt ccctacttat ttcgttttga gaattatcga agcaatctta cataaataac
     1501 taaaaaacaa aaaaccagaa aatcagtata ctgattttct ggttttacat ggtttgttca
     1561 ataaaaagct gttggaactg ttaatattaa attatcgcat cgttacgaat tcttccgctc
     1621 ctgttggatg gatggctact gtattgtcaa agtcttcttt agttgcaccc atcttgatag
     1681 ccacggcaaa accttgcagc atctcatcga caccaacacc aatagcatgt aagccgacga
     1741 ttctttcttc ttttccgaca cagatcaatt tcatttcgca tttttggcga taatcattta
     1801 atgcaaagta cattggggta aagcgtgaac gataaacttt caattcattt tcgccatatt
     1861 tttccaatgc ttcttcttct gtcaaaccaa ttgttgcaat tggtggatgc gtaaagacga
     1921 ctgtcggaac aagtttatag tcaagataag aatctttctt tccattaaat aaacgttctg
     1981 ataaacgtct accagctgcg attgcaacag gagtcagatc taatttaccg atcacatcac
     2041 caacagcaaa aatatgttca tccgttgtat tttggtattt atctactttg ataaaccctt
     2101 ttttgtccaa ttccacattt gtattttcca gacctaattt ttcggtattc ggtacacgtc
     2161 caccggcaaa aagtacacaa tcaccagtta aggtttcacc attttcaaat agcaccgtat
     2221 attcgtcatt gtttttagta atttctttag caatatgatg tgcgtaagtt tttattccag
     2281 cttcttggta catttggatc aaattgtccg aaagcatttt atcaaacgtt ctaagtggtc
     2341 gttcttttcg atatgcccac atgacttcac tacctaaacc acgaaataca cctgaaagtt
     2401 cagccgcaat gtatccagca cctaaaacga ttgctctctt aggtaactct gttaattcaa
     2461 aaaatccatt tgaatcaatt gcgtattctc caccaggaat ggtcatttta cttgcacggc
     2521 ctccagtagc gatcaagatt ttaggagcac gataggtttc accgtttact tcgatcgttt
     2581 gactatcgat aaaagtagcg taacctttta tagcctcgac atgattgcta tctaatcctc
     2641 tttgataagc accgtgtaga aaatcaatat atttttctcg tctttctact agaccggcga
     2701 aatcaacatt ttttaaatct gcttggatac cgtaacttga ggcatctcgt tcaattgttt
     2761 ccaaaatcgt acttgcttgc cacataactt ttttaggtac acaaccgacg ttgacacatg
     2821 ttccgcccaa ttcattcgct tcaatcagca aaacttttgc gccatgcatc cctgctctgt
     2881 ttgctgaagc aataccgcca gagccgccac caattacgat gtaatcatat atttgcattt
     2941 tgttcctcct tattgaagag atcaatcaaa acctttattt aacgatcctt tttctacaga
     3001 aaacaagtgt aacacttttc ttgaattttg tatgcgaaga tgcttaaaac agggatatat
     3061 cagctttttc cttatttacg ttttcggatc atatctccag gtttcaccgg ctgtgcgact
     3121 ggcatagtta aaatcatcat tggatttggt gctcggtcaa tggattcgcc ttcttcatta
     3181 tgcataactt caacggtttg actgaaatga ctgaatcctg gaccataaaa ttcgatttca
     3241 tctcctacag aaaacaagtt tctctgacgt atcgtagcaa tctgtgttgc ctcatcataa
     3301 gataatactt caccgacaaa tttatatact gggattttac gacgttcacc gaataattgt
     3361 tcgttttcag ttggtgtatt gtagtaaaaa cctgtagcta attctcgttg tgctactttc
     3421 cataactcgt cgatccattc ttgcttgcag acatagtttt caggatcttc catataactg
     3481 tctacagccg ctttatagac atttgacacc gtagatacat aatggatcga tttcattctt
     3541 ccttcgatct tgaagctgtc tacgccgttt tcaatcaatt ctggaatatg ttcgatcatt
     3601 gacatatcca ctgcactcat cgagaattct tcttccactt cgcctgtatc agttaaactg
     3661 tttcgttctt gaccaaaagg catatcgaac aatccatact tccagcgaca agattgagaa
     3721 catccgccac ggtttgcatc ccgcatcgac atatggttgg acaatgtgca tcgaccagaa
     3781 taagagatac acatggcacc atggataaat gcttcgattt cggtatccgt atttttacga
     3841 atctctgcca cttcctccat tgaaacttca cgagctaaaa cgacacgttc caacccttct
     3901 tctttccaga attccaatgt ctcaaaatta gttgctgaag cttgagtgga taaatggatc
     3961 ggcagacctg gggcttctgt cgcacaaatt tcgatcaaag ctggatcaga aacaatcact
     4021 gcagaaatac cgacatcacg caaactgcgg aaaaattccc ctgctccatc ttcatttcct
     4081 tcatgggtga ccatattagc agcaacgtat accttcgcat catgttcttt tgcaaaagca
     4141 actccttcag ccatttcttc ataagtaaaa ttacctgcac ggcttcttaa accatatgca
     4201 ttcccgccaa tatatactgc atctgctcca taatggatcg cggtttttaa cttttccagt
     4261 gttcctgctg gcgctaggat ttctggtcgt ttcaattttc tttttactgt catttctttc
     4321 atcatactcc ttatttgata gcagaaggat caatataaaa gaatcctgta tctaattcac
     4381 ggttttcagg atgcaattta ctgatttcgc tagtcatttt ttccgctttt tcttgatccc
     4441 agctaccttc taaaatttgt ttttttgctt cggcgaatag tcgagtgatg tttacgaatt
     4501 tttcgcctgg agtaaataat ccgtccaatt tccatgtaca gtaatgatgc tgataaagtt
     4561 cattcaattc attcgagaga ttcagatcat tgctggcaaa aatatgtgtg ccatgactat
     4621 cttcataaat agaataatgt gtctcttctt ttttaggttc cgagataaaa agacctcttt
     4681 cacgatcttt ttgttcgtct tgtttcgtat agttatagta attctgtagc agtggacgtt
     4741 tggattgatg gatgcatgta gcaccataca caagggtttc tacaggaata tcgagttttt
     4801 cttccatcgc cttcatctct tcaaaaggaa cttcacgagc aagtactgct ccaactgcgc
     4861 cttttttcgc ccagaaattg atttgacgtg cgctcgttac taatgtttca gcatcataaa
     4921 tatacggtat acgcaattca ggattttttt tcatcgtata aataatacct gtatcaccaa
     4981 ctgtgatcac atctacgttt atttctttta aaaaagccaa atattcaggt acatttttca
     5041 ttttttcagg atgaatgatc ccatttacag caatattcgc ttttttacca tacttgtgtg
     5101 ctaattctac caattgtcgc tgttcttctc gtgtaaatga agcaggcaga cgcaatccaa
     5161 attgctcttc accgaaatat agtgtatctg cacctgcagc aagcaactgt tctgcttgtt
     5221 caatagtttc tactgtggtt atgagttcga tcataatttt ctcctcctac aacttcataa
     5281 tagtagcata gctctaaaaa ctttccaata cttcttctat ttaaagtcgg atttttgaaa
     5341 attatttcac aaaaaatcaa aataaagttc gatatattct gtccaaatat aaaaagagat
     5401 tgtgacaaaa atcttgtcac aacctcttaa tcaagtctta ctattataaa aattcagcta
     5461 cacaggccaa gtaactatag ggaacctact gtttcctttt taggtccgcg aacattcaat
     5521 gtgatcttac cttttgaagc acctactgtt acgtggtcac ctaaacgaat ttctccgcca
     5581 agcaaagcct cgctcaatcg atcttcaatt tctttttgaa gtgcgcgacg gattggacgt
     5641 gcaccatatt caggatcaaa tccagctttc ccgatcacat cgattgcaga tggtgtgatt
     5701 ttcacttgaa tatcttgttc tttcaatcgt ttgataatcg ctttgctcat gatttggacg
     5761 attgtatgga tttcatcttt actcaatgaa tggaagacga cagtctcatc gattcgattc
     5821 aagaattcag gtcggaaagc tttctttaac tcttctaaga tacgtttttg catggctttg
     5881 tgatctttag taacatcttg gacattgaat ccgacatttt tttcttctcg aatttgtgtc
     5941 gctccgatat ttgaagtcat aatcatgatt gtatttctaa agtccacttt tctaccttta
     6001 gaatctgtta aatgaccatc atccaatact tgcagaagta aattgaaaac gtctggatgt
     6061 gctttctcca cttcatctaa gagaatcact gaatatggtt tttgacgaat tttttctgtc
     6121 aactgtccac cttcatcata ccctacgtat cctggaggag acccgattaa tcggcttgta
     6181 ctgtattttt ccataaattc tgacatatca acacggatca gggcatcttc acttccaaac
     6241 atgacttctg aaagtgcttt ggccaattct gtttttccga caccagttgg tccaaggaac
     6301 atgaatgaac caattggacg atcaggatct ttcaatccgc ttcgtgcacg gcggatcgca
     6361 cgtgaaactg ctttgacagc ctcatcttga ccaacgacac gttcatgcag aagtccttcc
     6421 agttctaaca agcgttcgct ttcttttttc tccaactgct gtagagggac accagtccac
     6481 tccgatacaa ctgtagcaac atcttcttct gttacacgat cagaataacc ttgctcctgt
     6541 tttgcttcaa ttgcagaaac tttttgcagc tttttacgta gtgctttttc ttgtctgcgt
     6601 aattgagcag cattttcaaa gtcttgtttt tggatcgctg cttccttttc ttgtactagt
     6661 tcatcgattt ctagtttgat cacagttgat tttgttaagt gatcggtttg atccaaacgg
     6721 actttcgctg ctgattcatc gatcaaatca attgctttat cgggtagttg acgagaagtg
     6781 atataacgaa cagaaagatt gactgctgct cgtacagctt catctgtgat ctccactcca
     6841 tggtgctctt catatcttgt acgtaatcct tgaagaatca cttctgcttc ttcaggagtt
     6901 ggctcatcta cttgaatgcg agcaaaacgt cgctctaatg cagaatcttt ttcgatatat
     6961 ttttgatatt catctagggt cgttgctccg atcgtttgta attctcctcg agctaacgcc
     7021 ggtttcaaga tattcgaagc atcaattgct ccttctgctc cacctgcacc aattaaggta
     7081 tgcaattcat caatgaataa gattacttga ccatcattat agatttcgtc gatgaccttt
     7141 ttcattcgat cttcaaattc accgcgatat ttcgtaccag caaccaaaga tcccatatca
     7201 agcatcatca ggcgcttttc ttgcatgtct tctggcactt cacctaaaat gattttttga
     7261 gctaaccctt cagcaatggc tgttttgcct acaccaggtt cacccaccag tacagggtta
     7321 ttctttgtcc gtcggctaag aatctggata agccgtttca cttctctcga acgaccaaca
     7381 actgggtcca gcttgttttc ttttgctaat ttcgtcaaat cacgtgctaa tgaatctagc
     7441 gtaggtgttc cttgcggtgt ttgccgttta tcttgtacat ttacatttct acgacgattc
     7501 attccttttc cagcagaatc accagctagc cccatttttt gtttcagaac ttgacgggct
     7561 tttgaaaggc tgatacctaa gttgatcatg attcgagtag ccatgatact ttcatctttt
     7621 aataagctca tcaaaatatg ttctgttcca actttttgag aaccagtttt cttcacttcg
     7681 ctgctagcta aagcaaaaac ttgtttcatt cttggtgaat atggaagaaa gactccttta
     7741 gggtaagaac ggataccacc ataaccaatc agatgttcaa tttcttcata aatttccgtt
     7801 tcattcaaat ccatttcacg taatactttt ccagcaattc cattttgctc gaccactaag
     7861 gctagtaaaa catgttctga actaactgat tgatgtttca accgttttgc ttcttcttgt
     7921 gcaatttcta atactgcttg ggctctttca gtaaaaagtt cttccatcta atctcacttc
     7981 ccatcttcat aacttaaacg ttccagaaat gctcggagaa tccgagcacg tgtatgatct
     8041 tcatattcat tgaaattcaa tgtgtttttt gcaattgcac tcaacatcaa gtttccttct
     8101 ttttttgtaa ttatttggtc ttcataaagt ttttgaataa tagcaaaagc atttttctca
     8161 ctgattgtat catcaaataa ctgatttatc tgctcaagta attgtttttt atctgaaatc
     8221 cttacttttg caattcgtat ataaccacca ccaccacgtt tgctttctac tagataacca
     8281 cgttggatgg taaaacgggt attaatcaca tagttgattt gtgaaggaac acagttaaag
     8341 aggtctgcca tttcaatcct tcgaatctcg atcatttcac tattttccag aattttcttt
     8401 atatatgatt caattaaatc cgaggtattc tgatgtgcca tcttattcat tccctttgac
     8461 taatattgac cttaattata acgaatttga taatagaaag aaagaagaac gctttttgtt
     8521 aaataaatcc tttagaatca acaaaaaaaa atacggaata tactattccg tatc
//
LOCUS       DAYYCY010000110         8495 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000110 DAYYCY010000000
VERSION     DAYYCY010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8495
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.110"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..150)
                     /locus_tag="ACHAZB_002029"
     CDS             complement(<1..150)
                     /locus_tag="ACHAZB_002029"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8614669.1"
                     /translation="MENHVLETGMQKITTHASITAKPFFEKRGYKVINEQTVELRGQL
                     FTNFLM"
     gene            complement(594..1715)
                     /locus_tag="ACHAZB_002030"
     CDS             complement(594..1715)
                     /locus_tag="ACHAZB_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFM8614670.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVVDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYRPG"
     gene            2043..3452
                     /gene="istA"
                     /locus_tag="ACHAZB_002031"
     CDS             2043..3452
                     /gene="istA"
                     /locus_tag="ACHAZB_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HFM8614671.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3449..4177
                     /gene="istB"
                     /locus_tag="ACHAZB_002032"
     CDS             3449..4177
                     /gene="istB"
                     /locus_tag="ACHAZB_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HFM8614672.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4305..5216)
                     /locus_tag="ACHAZB_002033"
     CDS             complement(4305..5216)
                     /locus_tag="ACHAZB_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM8614673.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5233..6240)
                     /locus_tag="ACHAZB_002034"
     CDS             complement(5233..6240)
                     /locus_tag="ACHAZB_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFM8614674.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6237..8366)
                     /locus_tag="ACHAZB_002035"
     CDS             complement(6237..8366)
                     /locus_tag="ACHAZB_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFM8614675.1"
                     /translation="MKKKLFRIVGIVFISIVTLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8371..8481)
                     /locus_tag="ACHAZB_002036"
     CDS             complement(8371..8481)
                     /locus_tag="ACHAZB_002036"
                     /inference="COORDINATES: protein motif:HMM:NF024254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8614676.1"
                     /translation="MMQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2599 a         1729 c         1486 g         2681 t
ORIGIN      
        1 cattaaaaag ttagtaaata attgccctct aagttccacg gtctgttcat taataacttt
       61 atatcctcgt ttttcaaaaa atggctttgc ggtaatggaa gcgtgggtag tgattttttg
      121 cataccagtt tctaaaacat ggttttccaa ttcatctgca attttagtcg caataccttt
      181 tccttgatgg ctatgttttt gtcacaactt cagtattcct gtaacaatac ggatttcagt
      241 atacaacaac agcttttgaa aagatacttg caaaaggatt aagatatcta tgacctgcta
      301 tgctcactga tgttgaaaag aatgttttat taggattgag gtaagcaaga tagtgaatat
      361 gtccggaaag cctgtaaaat aaggcttttc ggacatatgt gaagttataa aaagatagcg
      421 aaaaatctat ttagtatcac taatgttgcg aacatatcgt cagaatagtc tgacatttat
      481 ttcttgctaa aaatttcaaa aaagtcaagt gctgacgcag cggatgacta ttttccagaa
      541 aattttagta tgtattcaaa aatcaaacgt gacaacagct attaactaca tcctcatcca
      601 ggtctgtaac gtttgaacca ctcttctgcg ggctcaaaca ttaagtaatg gaaggtacgt
      661 ttcaattgaa acagcgtcgc tgtcgtattc aactcaattt ttatcacata agtgagaagc
      721 gttgcgatta aggtgaggat gacttgattt atcgcccctt gttcactatg tgaatatagt
      781 cgtttaatgg tcagattttg ttttaagtgt ttgaacaaca attcaatctg ccaacgggct
      841 tgatacatgt cggccacttc gtttggcgat acatcaaaga gattggtcac cattcgcagg
      901 attttttgtc ccttggtaag aaccgttacc aacctaaaac gctctgtcac atggttgaca
      961 ccgccgacaa gaatcacttg ttggtcactg attatcgtcc catcacgggt tgttgtatct
     1021 gctacttcaa tttgatccaa gatggtaatt tttgtattag attttgtccg tgtaacgaag
     1081 aaatagccgt cacgatttag tttgtctaac aacttgtaat caaagtagcc gcgatccacc
     1141 acatacgttg cttgcgtttt atcaacaaga acttcaaggt gattacggtc atgctcactc
     1201 gcatttgtca tcgtaaaact ttcaggatac tgattggttt tatcaagata acagagattc
     1261 aaatgtagct tgatgccaga ctttgttttc cgaaacttgg cccatggaaa ccatgctttg
     1321 ttcaacggaa tagtcgttga atcaatcaat tgtagactgg ttgtttttga gcttggctgc
     1381 tgagcagaaa ttctggcgac taattgcgta aaaatttcca tcaatacttc ttgcttgatt
     1441 tctggcgctc tacgagataa cgagctgtaa cacagggaat caatgccaag ttccttgcag
     1501 aggcgctgat ctaaaaaggc gcgatcaatc tcgcgatagc ttggcaattc ttcataaacc
     1561 gcatgaagta acactttaag cgtcgtttgg aagtctagtt ttttactgta gaaatcaaag
     1621 tcgctaatta agctttgaga gttttctgat aaaccagaaa aatttattgc agaaaaccat
     1681 ttttgaaatg aggtttttac gttatactta tccatgcgtg agtccttttt attggtcttg
     1741 gataagtcat ccgagaatag tataaaggac ttttttgcgt ttggatagct ttgtattagg
     1801 cttttgatgg gttaaaaagt ctttgcaaca ctagtaattt agtatttatg tataaattga
     1861 aagatatttt accaccactt agtcttctta aaagtgatcc aacattttgt ttgtggcaga
     1921 atttaactac acactaatgc agccgaaagt acacaactat gcagcagttt gactgcgaac
     1981 tggtttcctt ccatcataag tttgaatgac ttagtgatgg aggtacagaa aggttgttaa
     2041 ctttggatga tattaaaaca ataaaacatt tgagaaataa tcgtgatcag tcaattgatc
     2101 agataagaaa agcaatgaat atcaattggc gaacagcgaa aaaatacgcc gatagtgatc
     2161 aactattcat tacagatccc actcgaaaaa acaaatccat gatggatgat ggttggggag
     2221 aaatcatcgg tagttggcta gaagaagacc tacgtttacg cagaaaactt cgtagaacgg
     2281 ccaaagctat ccatgaacaa ttgaaacctt tgggtttccc tggctcctat cgaacagttt
     2341 gtgtatacat ttctcaatgg aaaaagcagc gttacgacga aacgaattac caagctgtcg
     2401 atcgtcttga tcatccgatc ggagaagcac aggtcgattt tggaacaatg gaagttatgc
     2461 atgaagatca catgaaagat atacaacttc ttatcatgtc atttccctat agtaatgcga
     2521 cgtccgcaag tgccatgcca gctcaaaacc aagagtgttt cctggaagca ttaaaacaac
     2581 tatttaatca atgcggcggc gttccaaaaa cgatcagaat agataactta actccagcgg
     2641 tgaaaaaaac aaaatcaaaa tttgaagaag cacaactaac cgatgaattc ttacgtttca
     2701 ccattcatta tggttgtgat gttcaagtct gtaacccacg tagtgggcat gaaaaaggac
     2761 atgtcgaaaa taaagtgggt tatgtccgat acaatttctt ctcgacttcg ccaatcatgg
     2821 actcttttga gtccctaaat aagcaacttg aagaacaatt aaaagcagat cgaaaaagga
     2881 cgcattattc aaaacatcaa ttaatcgaag atttgtggaa gaaagaaaat aaagcgttac
     2941 attctcttcc acaagaggat tacccaattt ttaaagaagt ggaagctaaa ctaaatcgtt
     3001 atcacgaaat gaaattggat caaacggtta ttcatattcc taaagcatat ggctattctc
     3061 aattgactgt tgttttgact tggaataaat tcaaagttct tacgcctcat ggagaaatat
     3121 taatgaacga atatcgcccc tatatgaaca aaaaaagatt gatcccatgg agtgacatca
     3181 tgaaacaatg gagtaataaa ttgaatgtcg ttagttattc gaggtattgg aagtacttgc
     3241 ctgcacgcct tcaaaattat ctagcgattg ataataatca cctgaaatac agacggataa
     3301 aacaattact aagtttatta gcgacctacg atatccaaca aattaatgaa caattttacg
     3361 aattagtcgt tcaagaagac gccccctctg actttgatgt gaattggtca aactatgatg
     3421 cattaacaaa aacaggaggt gtgcaaaaat gattggacag acgttagaag attattgtaa
     3481 aaagcttcga ttaagccata tcatcacgaa cataccaaga gcttctttcg aagctccaga
     3541 acaatttatt accgatttat tccgtttaga actggagacc agacagcaga tcaaattaga
     3601 acggatggaa aaattagcca aatttcctgt accaaaatcg ctaaagaact atgaatggca
     3661 cgaagatatt tttctaccat ctcatctaga taaggagacc cttgtctctc ttgattttct
     3721 aaaaaggcaa gaaaacattg tttgtgttgg ctctcctggt acagggaaaa cacatctagc
     3781 gatcgcatta gggagaaaag cctgtagtga aggtgtttca accaaatttt tccgagttaa
     3841 caatctcgta gagcaattag agaatgcatt aaaaaaacaa cagctagcca gtttcaagaa
     3901 gcgattcgaa acaacagaat taattattct tgatgaaatg gggtacttac cattgacaaa
     3961 agaaggagct gaacttctct tccaactgat ttcagattgg tatgagcaaa agagcattat
     4021 tattacctct aatttagaat tcagtcaatg gaatcgagtg tttatggatt ctcgattaac
     4081 agctgctctc gttgatcggc taatccatca cgctcacatt atcagcttca aaggcgaaag
     4141 tttcagattg aaaaattcat tatctaaatc atattgaagg aaaaaactag ttgcagcaaa
     4201 actctgcact ttcggctgca tttttttgta aaaagctatt gcacaacaca aacatttgaa
     4261 cgacgtaatt ccaaatgttg gattgctttt agataatctt ttatttattc acaattgcta
     4321 atagtccaat tttcattttt gtatagtttt aatctatact gaaaaacact tgttgttttg
     4381 gacaactcat caagatactc aacaccaaca atcacttgaa tcgctttacc cttttgtttg
     4441 taaataggat tcactaattc cacaaatttc aaattagtat taattggttg catcgcgtct
     4501 ttttcaacat aatgctctag ttctttttct gaggctgctg gataaagcct gaaaaacgtc
     4561 tctaaaaact cggtgatttc ttccgttgtc tttgaatcaa tttttgaatc actttttact
     4621 tgtcttactt tatgttttgc cttttctggc ttaccaatca tagtgggtaa actcgttaca
     4681 atatgattgc ctttttcatc ttcaaagatc atcagacgat atgcagaaga aattgattgt
     4741 gtctttttcc cttctgtaat cttttgatct accacaaatg aaacgacaaa ttcttccgag
     4801 tgtttttcaa catctagaat ttttactgat acaacctcgg aactagtggg aatatccgct
     4861 cgaaccatat cttggctcaa agcgagccct tcttctgtaa ggtactgctc aagattagtc
     4921 attctctttt caattacctc cttgttattc ttccatgaaa aatattcttt cacaaaatct
     4981 acagtaaaat tttctactcc agaggtattc actattttct ctttcacgac cactttctca
     5041 tgagttgtat gctgatcaat ggccgtgaag tttttgtaga ttccaaaagc aaggctacaa
     5101 gacaataaaa gccaaagggc aagcaccatt tttcgatgct tgcccacgga tactactcgt
     5161 tctttcttct tttttggaat cttttccttg ttacttcgct caatttttat cttcattttc
     5221 cattcctctt ttctattgtt tataccgacc agcaccaata atgtgctgtt gccaatagct
     5281 atcggtaaga ttggtccaac ccaccggatc tcctgcatga aacattctgt tctctcctgc
     5341 ataaatgccc acatgagtca cataagtacc ggcatcataa gttgaatgaa agaaaatcaa
     5401 atcgcctggt ttggcatctt ttaagtcaat atgagtcata gcatcatatt gatcctgagc
     5461 cacccgaggg agtttcaaac caactttggc ataacaccat tgagttaaac cactacaatc
     5521 aaacccagtt ccgggacttg acccaccaaa tacataagcc gttccttcat acttgaacgc
     5581 ctcgtcaaaa attccttgga ctgttttatc atcaaactgt gttgtagtta ggtactgctt
     5641 cactaataag caataaaaca tattgccata gttataccgc cacccactat tttccttaat
     5701 cgcgatggga ttgggataat cgactttttg accacccgat ttttcttttg aaaaagattc
     5761 ggctaaagca aaactatgct tctttccgtt tttcgcgaca taatccaaat acccgtcacc
     5821 atagttataa gcctgaatga tactatcttg atcgcatcct agttctttgg aacacttaac
     5881 caaagacgaa aaatagagac acacttgttt gatggatgcc tcggtactta atgaattcgg
     5941 tgataaacct gctgattcag aactttgcat gacatcttcc attgtacctc cagattcgac
     6001 ttgaataatc gccaaaagaa tcgaaacata ctctgttata tctgattctt tggcatactt
     6061 ttccaccatt gaacggtgtt tcaaaactac ttctgagaca ctactacctc caggagaagt
     6121 tccagaacta ccacttccct caccgtcctc accaaacaac aaacccacac acatcaagat
     6181 gcccacaaaa gagatggtca ggaaggttaa cgaaataata ctcaattttt ttaagttcat
     6241 cttttctcac ctggctttgg ttttgggcga gtgttctttt ttctttgtac ttgttgattg
     6301 gttcgctttg gaaaagtgaa tctctgtttc acttgttgaa aggaattgtt cttgcttagt
     6361 ggctgagtgc ttccgtttgg aatgacttct tgactagaaa gtttccgttt gatttcagga
     6421 ttcgaatgta cttgtaccct tggttttaaa gacctatttt ctggtagttc atttttctgt
     6481 ggttttatct ccttttttat cggaacattt tcccttcctt tctcatgacg attccatgct
     6541 tcgttcaata ctttgcgttt ttcatccatt tttgcgtctc ttcgttgtcg ttgtttttct
     6601 cgcaactccc ctcgatttgc tttttcttcg aaaggccctc gcttaaagtc ctctggtact
     6661 tttttcgtct tttccatgcc tttatgaagg ctgtatttca agttagttgg tgcatcggtt
     6721 acttgatttt ttgcttgttt cgctttgtcc ttcacgattt cttttgtatc gagtactttt
     6781 ccaacttttc taccaactaa atttattcgt gattctttct tcgtgcccac tggttgttct
     6841 tgttttttca gatcagaatt tagctcgtta atgcttctcg cggaatggtt ctttctattg
     6901 gacactctat tgatttttga actacctttc ccttttgtag aactggtagc tagtttccca
     6961 ccaactgctg caccgccacc aaaagcaaaa gttcgaccca catttcgatt aagctgtcgc
     7021 aattttcgat tcatcaacat ttgcggtttc cgcatgactc gccttcccat tgactgacta
     7081 tcattacttt gaaggttaaa catgctcata atgtcaccta atttgaaata gataccggca
     7141 aaggtcacaa tttgtagaaa agctaccata aagaagggat aggtggccga aatattgaaa
     7201 aacatcgtgg agatactgaa tgcagtcgta atgactaagg taactcccgc tcgcatcata
     7261 atcgtattga acaaacgaat aatcgccctt ttgcccaaac tctcatacgt cggcaacatc
     7321 gacaataaga aactaatggg cagaaacatc gcaaagataa tgaaaaggat ttgtgagaac
     7381 aacataatcc ctgtcagaag aaacacaaaa aaggaaatcc caatattaaa caacactaaa
     7441 aagaccacgg ttcctaaacg gttcatggtc ttggtaatac tgagattcgc attgtcatta
     7501 tcttcaatct ctgctttgac tgcttcttcc cgatccttac ccttattttc atccggactc
     7561 acggataaaa tcttattaac ccgaccctcg ccaatttctt ctatattgga atcatcgaat
     7621 tgtaatagta accagggttg ttgaacttga atcgaaaata aactgtctcg aatcaaatcc
     7681 acactatctt ttccctggct ttctgaatta ggtacaacaa tcttggttcc aaggctaaga
     7741 gccgcttcac taacgtctga gctaaagtca ttgatttttg taatataggt gggcgcatac
     7801 gcaatgaaag aacccgagag tagaaaaatc atcacaaaat taatgacggt tcggactgct
     7861 ttggtagttt ctcgttttaa taatccggta tacgccacat aaactcctag agctaaaatc
     7921 atcaataata ggaacccaaa gtagaatccc gaagtctgta atccattttc agtgataccg
     7981 gctaaagttt ggatattctt tccaatactt ttagccgtat ccgaaatgaa atccaacttg
     8041 taagcttctt gaaccacata gcccgtggcg tttgataaat acagacttac tgtccaaata
     8101 aaattggtaa tggcataaag accatacatc acgcttttcc ctaagccatc cccccaattc
     8161 caaggtaacc aatcccaaga actatccaca aagaaatcca gttgataatt gttcagagaa
     8221 tatttggaat aaaggtttcc ggcttcaact gtatcatcaa ccagacctgt cgcctcagct
     8281 accgtaccga ctaaactcaa tagaactgtc agagtgacga tgctaataaa aacgattccc
     8341 acaatccgaa aaagtttctt tttcatcccc tcactccatt ctctcttgaa ccggcggacg
     8401 agtgtcaaaa gcatggaaga gttcttcaaa gactggatga acttgaacca cacctactcg
     8461 cccatacaag tcttgcatca agcattgacc gtttt
//
LOCUS       DAYYCY010000111         8159 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000111 DAYYCY010000000
VERSION     DAYYCY010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8159
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.111"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            251..2086
                     /locus_tag="ACHAZB_002037"
     CDS             251..2086
                     /locus_tag="ACHAZB_002037"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFM8614677.1"
                     /translation="MQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFFFLL
                     LLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVSPVL
                     LIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHKNSP
                     SFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMKKYN
                     TKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTGGLM
                     LSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIAIHP
                     FTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQSNT
                     ETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAVKKF
                     IQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAATLNK
                     EVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVFVTE
                     DSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(2146..2862)
                     /locus_tag="ACHAZB_002038"
     CDS             complement(2146..2862)
                     /locus_tag="ACHAZB_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFM8614678.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(3089..3631)
                     /locus_tag="ACHAZB_002039"
     CDS             complement(3089..3631)
                     /locus_tag="ACHAZB_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFM8614679.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(3636..3863)
                     /locus_tag="ACHAZB_002040"
     CDS             complement(3636..3863)
                     /locus_tag="ACHAZB_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HFM8614680.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(3942..5822)
                     /locus_tag="ACHAZB_002041"
     CDS             complement(3942..5822)
                     /locus_tag="ACHAZB_002041"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM8614681.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(6011..6625)
                     /locus_tag="ACHAZB_002042"
     CDS             complement(6011..6625)
                     /locus_tag="ACHAZB_002042"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8614682.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            6767..7327
                     /locus_tag="ACHAZB_002043"
     CDS             6767..7327
                     /locus_tag="ACHAZB_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8614683.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
     gene            complement(7632..7946)
                     /locus_tag="ACHAZB_002044"
                     /pseudo
     CDS             complement(7632..7946)
                     /locus_tag="ACHAZB_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
BASE COUNT         2478 a         1617 c         1320 g         2744 t
ORIGIN      
        1 atttccaaac tcagggaagg catggctgat aaatatcggg cagtttttta tttgatattt
       61 attagcagtg tccctcctag ttatcttttt attttatata caaaaaatcc gtttcattca
      121 tcgtttcatc gaatacttcg cttttgattc gattgaacaa ttaactcagc gttggaaaaa
      181 aagaattcat tggctttttg ttcataaaag ttatttttta gttgcagcat ttttttactg
      241 ctttaccttc gtgcagattt tcgttttaga accaaaagaa ggttggaaag aaactatcca
      301 ggttgcctta aaaatattta ctcagcgaca agcacccatg atcctgacaa tcatcattat
      361 tatgggattt ttcttcttac tattgttgat ttccaatcgt ttttggtacg ccttgactgc
      421 taccttgatc attaacttac tgctcactat ttcgacagta atcaaaatgg aaatgcggga
      481 agagcctgtc tttccttcag atttaaaaat gctgaccgga ttgtcagaac tattatcgat
      541 ggttagccca gtcttattaa tcgtgggcgg attgatcctt ctcctattat tgattactag
      601 tattattgta caacgtagac tacaaaaaca gtattccttg aagattcatt ggaaacgtcg
      661 atttatcagt atcgctgtat tattaggatg tttttcgggt gtctttttca ttaatcacaa
      721 aaattcgcct tctttcttgc tgtttaattt atttaaagta aacaagactt tctttaacca
      781 gcaaggcgcc gtaaaagata acggaccagt cattcaattc ctaaacaata tcgacattaa
      841 aatcatggaa gaacctgctg gctattcgaa agaaaaaatt aatggaataa tgaaaaagta
      901 caacacaaag gcagatgaaa tcaaccaaac aagaagtgac tggttaaaca atcagacgat
      961 cgttctcaat ttaagtgaaa gcttcagtga tcctgcacgt gtccctaatc tgacagtacc
     1021 tacagaccct attccttaca tcaaaagcat tataacagaa ggaacaggag gattgatgtt
     1081 atccgtcggt tatggtggcg gaacagctaa catggaatgg gaaggactga caggcttgag
     1141 tatcagtaac ctgtctgctt cgttagttac accttatacc cagctagtgg agcgtcagaa
     1201 aataagtcca aatattaccg atctattcga tgaaaaaata gccatccatc cttttacagc
     1261 tgctctttat cgcagaaaac aagtgtttga taaatttggt tttgacaggt tctattattt
     1321 agacagtccg gataaattaa cttatacgga taaaatagaa caaagcccac gcatatcgga
     1381 tgattcagca tacaaagaaa ccttgaaagc tttacaaagc aatacggaaa cgtcacaatt
     1441 catccaatta tccactatgc aaaaccatat gccttatgat gattactata gtgaattgaa
     1501 ctacactgct gaaggaactg ctgtcattcc taatagaaga aaagaattac aaacttatat
     1561 gcaaggcctt cactataccg atacagcagt aaaaaaattc atccaagaaa tcgacaaaat
     1621 cgataaaccg attacctttg ttttttatgg tgaccattta cccgcaatct atagtgggaa
     1681 tgattcgaaa aaatatggat tagaacagca ccagacagat tatttcattt ataacaacaa
     1741 gtatagtcgt gaacaagcag ctacgttaaa taaagaagtt gttgctccta ataactttgg
     1801 tgctatggct cttgagcatg cgaatatcaa agtaactcct tactatgcgt tgttgacaga
     1861 agtatctaat catctacctg cattaacaat tgatcctaca gaaagcttgt ctaatcgttt
     1921 taatggaaaa caggtctttg ttacagagga tagtcaaatt atccaagaaa aaaatttaac
     1981 tgagaaacaa aaagaaattc tagaagatta tcgtctgata caatatgatc ttgttgcagg
     2041 taaccagtac agtgcaacat gggcgacaca aaaaataaaa gactaacaaa taaactgagc
     2101 aaggggttgt gataaaagac atgtcacaac cccttgttta tctcattaat ttgcttgtcc
     2161 ataaacgatt tgattaaagc catttttcaa ttcattattc gctgtctcgt tgaagtaaag
     2221 ataattatcg ggattcccct gattttgttg tgtcaccaat acttttggct gattttgctg
     2281 aaacccgaat agatagacgt gctttttcag atatggttct gtctctgcat cggaatagac
     2341 ggctgtgatt tgatagtctg cttgacctgt cccactttct gaccattctg cagaaacagg
     2401 cactccacca atagccatct tccactcccc gttcaaaata gcttgaggga ctttaagacc
     2461 atataagtcc acactcattt gattcgtata acttttgtat tgttgtccca ttgtttggcc
     2521 ccatttggtc ataaatgcct ctaattgtgc ggatttgtct gcattccaca gtgtttgttc
     2581 aacttgttcg ctgctttctg aagaggaaga tgaagcctct acagaagaac ttgaagtaga
     2641 tgtttgagtt gtttcagtcg ttttggaaga ctctgacgaa gtagaggtat ctgctgtttc
     2701 tgtacttgct gcagttgact tgctctctgt gctagttgtt gtagtagttg caactgttgc
     2761 tttcgctgtt tcttttgttg attctttttt cgattcattc ccgttggtac aaccagctaa
     2821 caaaaaagct agaccaattg ttactaaaat cctttttttc aattaaaaaa ctcctttcga
     2881 ttactcccac ttctttattc tatcaaataa gcaatgtgat tttattgatt tttcgtattt
     2941 tctcatcaat ctttcaaatc ttctaaaaac tgagaagcac ccgctgatgt tgtctgtaat
     3001 acatcagcgg gtgcttcttt tattttaatc ttcgtgattt tccaatttac ctcacgcttt
     3061 atatttgtat ttgaagaaac atagactttt agtcatcatc gtcttcctca tccaaatctt
     3121 gtcggacagt ttttccttgg agtctttgta cttggtagat gatttcttta ttgaaactta
     3181 aaagttcaac aagcaattgt tgcaagccat atttttcttc tttttcagct aatttgattg
     3241 cccgtgtgat aaagacattt tggaaatcaa aatcttttgc aaagttttcc aacattgttg
     3301 aggctggtaa atatttgttt tgaccatgtt cgtaaatttt tccataacgc atgacttctt
     3361 ctaaagtaga cggaacgatt tcattttcaa tcactaactc atgagagatt ttattgaacc
     3421 atctcgcttc ttgatcagca tattctgaaa aaagggtact taattcttgc gaagatgtac
     3481 ccaaaacata caattgtgct tggatcaatt tcatttcttc taatttaaaa tttgctaata
     3541 catgccccac catggcgcca gcagttggtt tatgatggtc gatatctgct tgttttgttt
     3601 cttgaacaaa tttttcttcc actatattca ttgatttacg cctcttttac ttttgctgat
     3661 tggacttcat aacctaattt ttccacaact gcagctaatt gttcagggtg gacttcatct
     3721 ggatcaaagt ttgctttgat tttgctcgca ttaaacaaaa ctttcgtatt gcttacccca
     3781 ggctgttgag aaaccgcatg ttcgattttt tgcatacagc ttggacaagc tagtgtatct
     3841 aattgcataa taattttttt catcgtagtt cttccttcct ttttcttgat agtcttattc
     3901 tagattacag cttccataaa aaaattgatt cttatcaagt tttacgattt ttcttctttg
     3961 ctttcgtgtc caaaatacaa taaacgcatc gcgttaaaga taacgatcaa gatactagct
     4021 tcgtgaacga acatcccact agccatgtaa ataaacccgg caaataagcc gttgagtaaa
     4081 aataagacga caccaatggc aatcactaca ttttcacggg tatttaaaac agttttctta
     4141 gccaagcggt aggcatgaac caacgattca aaattagatt gcatcaagac aacgtcactc
     4201 gtttcgatag caacatccgt tcctgatccc atagcaatcc cgatatcagc cgcagccaaa
     4261 gagggagaat cattgattcc atctccaata aaagcaacgc gcaatccttg ttcttggtat
     4321 tttttcacaa attgaacctt ttcgtctggt aataattcag catggacttc atcaatgcct
     4381 aacatatcag ctacataatc agcagtcatc tgattatcac ctgtcagcat cactaactgt
     4441 tttgctcctg cttgtttcaa cttagctaat tgttcagcga cttctggtcg aatcacatct
     4501 gacacaccta tgatttgagt gatttttttg tcgattgcta caataatgac cgagctgcct
     4561 gtttgttgta attgatgtaa atcttgtact tgctttggtg tgagagaaag tttgtgagcg
     4621 gcaatcaatt tggtgttgcc cgcacaaact gtatgaccat caatctcagc tgtgattcct
     4681 tgtcccttga ttgtctgatt atctttgact gctaattgtt gaaaatcaat gtctttctcc
     4741 tgagcataat ggatcacggc gcgccccaga ggatgatcgg ataacgtttc taatcgagcc
     4801 actaaagcta agctgtcccc ttcatcctct gtatagttct tgatagtgga tacagcagta
     4861 gttcctttgg tcaatgtacc agttttatca aagacaaaag tatcgatttt ggaaaaagta
     4921 tccattgctt caccgccttt gactaagatg ccccgtttcg ctccattacc gattcctgct
     4981 acattcgaaa caggcgcacc aatgactaaa gcgccaggac aaccaagaac caaaacagtg
     5041 attgctaaac gaaaatcacg actaatcaga ccaacaacca acgcaataag taaaacggtt
     5101 ggagtataat actgagaaaa tcgatcaatg aatttttccg catgggattt ggtatcttgt
     5161 gcttcttcta ctaattcgat aattttggca aaggtcgtat catctccgac cttagttgct
     5221 tcaatcgtca cgtagccatc tgctaaaatc gttccagaga agacttcatc tccactgatt
     5281 ttgacgactt gtcgtgattc accagtcact gcggcttcat ccgcataccc tttcccgtca
     5341 atgactcgtc cgtccacagg aatagctcca ccagttttta ctaagacgat atcgccttct
     5401 tccacgtcat cgatttccac ttcttcagtc gttccatctt cttggacaac actagcagta
     5461 gatggggcca tctcggtcaa agccttgatt gactcccgcg ttttggtcaa tgttttttgt
     5521 tccagatagc taccaaacaa aaacaagaat gtaacgatag ctgactcctt gtattctccg
     5581 atcataaagg caccaagcac agcaatagta acgagtaaat caattgagat aactttgact
     5641 ttcactgcct gatacgcatg gatcataata ggtatgaccg caataataga agctatggct
     5701 agagcaatgt tatagaacaa gacaaaacca gtaaattttc cgataaaacc aaaaataatc
     5761 aacacagctg ataaggcggt gatatgattt cgatgctctt ggatccactt ttgtaatttc
     5821 atctttctcc ctcttttcct ttcattctta cgagactaag tatagatact tatccatcat
     5881 aaaaaattga ccactatcaa gtttagtcaa aaaggcggtt ttcttttggt agaatacact
     5941 acaaaaaata gttagcaaga agactcacta aaagtgatct tgccaactat tgtcgtttct
     6001 atcgaaattt tcattgaatg attttcctgt acagcataca tcggagatct atttctttat
     6061 ctaagatttt tatcttgcta attttttcct ttagtctttc aaatccacgt cgttcataga
     6121 attgatacgt acagccacta tctgtaaaaa ggaatatttc ttttcctttc tctctttttt
     6181 caaattctgt tagcaacttg gtaccgatgc cttttgtttt caaatcagga tttgcagcta
     6241 aaaaaatgat ctctccatct ggcgaatgct tctttaaata tgctgcaagc atttgttcat
     6301 ttgctgtctc gtaagctcct ttactttctt tagcaaatat attttgtaaa aaatcgaata
     6361 tcttaacata tatttgctgt ccaaaagaac gatgtttctt tttttctcct tttatctccg
     6421 ccagcaaaac acctgccaac tcgtcctctg catacaaagc aagaatttga gttgctcgat
     6481 tcaactccat atacaaaaaa tatcttccgt aaagattcag cagaagttca ttttccatat
     6541 atctctcaaa gttcattcct tcaacagcaa aatagatagc ttttttgtaa tctttctttt
     6601 ctaagggttt aaaagttagt ttcatcattt cgcctcctca acttcaatga ttgtgttata
     6661 cttacattat aatagacaca gtgtctacta tctataatca atcatctata agtttgtcga
     6721 ttaagcgaca gctttgcttt gatatgtcgg ataaggagat aggtaaatga aaaagacaga
     6781 tagacgcgtc aaaaaaacag aaaaagcttt ggctgaaaca ctatccacat tattagtaaa
     6841 taaaaagatt caagcaatca ctatacgcga actaacagaa acggcagatg tacatcgttc
     6901 gactttttat acacattata aggatatcta tgatctatat gatcaattag aaagcaactt
     6961 ttttcgggat ctaaatgaaa ttctctctta tgatccagcc catagctatg aggaactata
     7021 cactcgcttg attgactatg tttacgcaaa tactttttta tatcgcttat ttatcctcaa
     7081 ccatggtgat atttcgtttc agaagaaagt taaacaagtt attgaagaaa actatctgaa
     7141 catatggcta tatgaagaca agaaaacgat tatcacagaa gaaatgagat tctttactac
     7201 gtaccacgtc caaggttgtc tatccgttct caatcggtgg gtagaaagca attacaagta
     7261 ctccaaaaaa gaattattag aactcttaca gaaactgaat gatcacatag agaaaatcat
     7321 gccttgaata aaaacatact aagcttagtt aagaagaacc gtaaccttct cgtataacaa
     7381 ctgaatctta tttagccaaa ttgtttactg aagatttttt gagtaaaatt tctataattc
     7441 ctacttattg aaaagtagtt tgtagtgctg cttctccctc tctaagtgat atatcttgtt
     7501 gtaccaatca aattcacctc atcattaata aattactttt aggctaattt attacaactt
     7561 ataggttgaa gctgtaacca atttatagtc atattatatt tttatcatcc agtttattga
     7621 attcttctcg tttacctcaa ttcattttca aggggaatca gctcatcatc taacatttta
     7681 cgccatgctc gcttcatttc ttgtgggtat ttaaatggtt tcgcttctgg atgtttggct
     7741 ttatatcctt cgtaatcctt actgttgata attgctttca tattaggaat tggatcttca
     7801 cctggcatat attgcagatt agaagcatgt aattttttct cagaaacaaa ccactcattg
     7861 gaaaagacat gaatcaaatc atcacttact tgtgtaaaat agcgacgttt aatgacaaag
     7921 atatcttcat tgctatctag tgtaccttca tttctttgtg cttaaattca aagataattt
     7981 tctcaataaa gatttttgat tccagtaaga ttcctttact ccttttacaa catagcgttg
     8041 tcctttgttt tgaaacatct tcttcccctc tttgccatta aaaatagccc aattctctat
     8101 aaagaagatt aagcttcatt attataatat aaagtttaaa tttggtttat tacttactt
//
LOCUS       DAYYCY010000112         8086 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000112 DAYYCY010000000
VERSION     DAYYCY010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8086
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.112"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            384..572
                     /locus_tag="ACHAZB_002045"
     CDS             384..572
                     /locus_tag="ACHAZB_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFM8614684.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            650..1663
                     /locus_tag="ACHAZB_002046"
     CDS             650..1663
                     /locus_tag="ACHAZB_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8614685.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYWGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(1730..2764)
                     /locus_tag="ACHAZB_002047"
     CDS             complement(1730..2764)
                     /locus_tag="ACHAZB_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8614686.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            2879..5104
                     /locus_tag="ACHAZB_002048"
     CDS             2879..5104
                     /locus_tag="ACHAZB_002048"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFM8614687.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            5122..7005
                     /locus_tag="ACHAZB_002049"
     CDS             5122..7005
                     /locus_tag="ACHAZB_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HFM8614688.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            6986..7960
                     /locus_tag="ACHAZB_002050"
     CDS             6986..7960
                     /locus_tag="ACHAZB_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HFM8614689.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
BASE COUNT         2612 a         1472 c         1708 g         2294 t
ORIGIN      
        1 tagttagtct acattatttt ggcgatttgc caataaggac tgttgagttg tgcaaaatca
       61 aatctgggtc agaatgaacg tcattctgtc tcagattttt cgttatttca atttgattaa
      121 ctgattgatc aaaaagattt ttattttcat attctcaaat gattcaacac taaatataca
      181 tgtttgacgc ggaaattatt attcgatttt ggcggcatca ggggggatga cgacgtagca
      241 gacccattat tttactcaag aaaatgtccg tgcctattat ttagaatacg atagtgaacg
      301 tgctgggagt tttgttcttt tagagaaagt ctctgaagat aaattagttg tcttagattt
      361 tgactgtaac gaaaaatggc gaaatggaag agcaccagca agtgattgac cggatctacg
      421 aagcgacacg atatctgcct ttggatcgtt tgtgcttaag tcctcaatgt ggatttgctt
      481 caacagaaga agaaaatgtt ttgtttgaag aacaatggaa aaaatttctt ttgtcaaaag
      541 cattgcagaa gaagtttggg gaaaatcatt aaatcatcta ggagagcttg ataggaaata
      601 acactatggt ataatcagaa tgcttaatac taactactga gggagcctta tgaaaaagaa
      661 aattcttaaa tttatcggat ggttcttcgg aatcattctt gggatcgcgt ttgcaatctt
      721 tcttgccttc cagctttccc ccagacctgg agcattcttg atcaaccata tgttttcaaa
      781 tactgttcaa atcactgata aaaaaacgtt cgatcaagca gaaaaaaacg tggcgaaaaa
      841 aacagatttg acgtatgagt ccaagtataa agagaatact tatgacatct actatccaaa
      901 aaacagcaag gggcctgttc ctgttctatt ttgggtacat ggtggaggat tcgtcggcgg
      961 agataaatcg ggtgtcaaag aatttgcgac aaaattagtt tcagacgcca acatcgcagt
     1021 ggttgcgatg aattatgaat tagcgcctga ttcagaatac cctaatcaag ttcttcaagt
     1081 gaatgaactg atcaaagatt tattagctga agaaaaagac gacaaattgc tagacatgga
     1141 acaattattt tttggtggag atagtgcagg ttcacaaatc gcattgcaat atgcagctat
     1201 acaaacgaat gatgcgtatg ctaaacaaat gaaaatcaaa cagctacttc caaaagatag
     1261 tctgaaagga accatttctt attggggacc tgttgatttg aagctaacag cacagcagca
     1321 ttcggatgat cgatttatga agtttttcgt caaaaccgtt gcgtggtcgt tgattggaac
     1381 aaaagattgg aaaacgagtg agcaattgaa agaagctagt gtggctgacc atgtttctaa
     1441 ggaatttccg ccaacgtata taactgacgg aaatgcatac tcattccagg agcaaggtca
     1501 agcattagaa aatcggttga cagaattaaa ggtacccgtg caatccttgt tttatacaga
     1561 tacaaaaaaa gagattaccc atgaatacca atttgattac acgctaaaag aatcgcaaaa
     1621 ttgttaccag cagacattgg attttgtgaa taagtataag taagaggttg tgaaaaagcc
     1681 gtttcgtttt gagtatgaag aaacgaagcg gcttttcaca acctctcttt caataccttt
     1741 ttgttcttgg gataaaactt tccctaacaa tcaatcgtgc aggataagtc aaaatgattg
     1801 gttcgtcccg tataccgtca attcgctcca atgccattcg aacagctgct tgcccaattg
     1861 atttcgcatt gatttgaaca ctggtcaagc caggggagag gtttgcgatt gtctcgatat
     1921 tatcaaagct tgctacaaca atctcttctc caatttggat atttttttgt ttcaatgccc
     1981 gatagacacc aattgccatt ggatcgctcc cgacaagaat ggcagtagga agaggggatt
     2041 cattttctaa tagattagtc gcaagtacct caccttgttt ttctgtccag ccacctagat
     2101 gataagacat atatttttct aagtgatgat cgatcataaa atgctgatag gcacgcatcc
     2161 tcttttcatt tatagaaatc tgcttcccac cgaattcatc catatcgact ttatatcccc
     2221 cgatataggc tattctttta tgaccatttt ctaagaacaa ttccagtacg gttttagtca
     2281 tccgttcaag atctgcatac accatatcga cctcttgctg gatgtcaggg ttatccacga
     2341 cgactatttg catgttttgt tttaacaagg cggtaatcga ttctttcgta acagtcccaa
     2401 caacgatcac cgcacctaac tgatctagat cttcccattg tttaggatta tcagatagat
     2461 tatagattcg attcatccct aaatgcagac gtcgagcttc ttcttccatc cctactcgca
     2521 aatgtttgta atagatatcc tgttcttcgc tcgctttgcc gatcgtagag atcacaccaa
     2581 tcgaaggcat tctctttttg atatatttac gagtactgat gtcataatcc aacgcagcag
     2641 ctgcctctac tatccgttgc cgagtagtct gagccacatg gatcgtctga tcgttattca
     2701 acacacggga aactgtagca ggagagacat ttgcctttcg tgcgacatct ctaattccaa
     2761 ccatttttac acactcctca aaaaaataaa aaatttttat tgacaaaatt gtttagtaaa
     2821 cagtatacag gaattataaa aaaaagtaaa cggtttccgt tttgttggga gtgaaatgat
     2881 ggatattacg catagaaaaa cagataaaaa tttcatctta tttgatgaaa agagccgagt
     2941 cttccattta cgaaatacat ttctttctta tctgataaag atagaagaaa gcaatgtatt
     3001 ggcgcatatt tattttggca aaccagtcaa gcagtacaaa gacaataaaa attatccccg
     3061 aagagatcgt ggattttcag gaaatgttcc cttaaatcct gaccggtctc tgtcgaaaga
     3121 tacactaccg caagaatact cttctcatgg cagcatggac tttcgaacgc ccgcatcgat
     3181 cattcagcgc aagaatggat cagacttact ggatctgaga tatgacagcc attatattac
     3241 agatgggaag ccagagatcg aaggcttgcc tcaaacttat gtgatggaca agtcagaggc
     3301 acaaacgttg gtgatttcac ttaaggatag agaaacagcg atttatttcg atttgttcta
     3361 taccatattt actgatagag cagtgatcac acgatctgta aagataagaa atgaaaccgg
     3421 agaaacaatc aaactagaaa aagcggctag tttccagcta gactttgccc atactaggcg
     3481 atttgatgaa gtcattgctt tgccaggagc acatgtcaat gaaagacaga tttcacggca
     3541 atctgtttta tctggaacaa aagtatttga aagccgtcga ggaacgagca gtcaccatat
     3601 gaataacttt attgctttag ttcatcatca tacgacagaa aatactggag aagcgatcgg
     3661 acttcaattt gtttattccg gcaaccattc tttcgaactt gaaaaagatc agatcaatca
     3721 attgagagtc gtcggtggga tcaacagcca tcgtttttcg tgggaattga atgcaggcca
     3781 gtcttttcaa acacctgaaa tgatcctttc ttactcaagt cagggattga acaaaatgag
     3841 tcagatccac catgagttgc tacgtgaaag aatcgcaaga ggtcgtcatc aatttgctga
     3901 acggccgatt ctggtcaata actgggaagc cacttacttt gacttcaata gtgaaaaaat
     3961 caaggcgatc attgatgaag caaaggagct aggcatcgaa atgttcgtat tagatgatgg
     4021 ctggtttgga aaaagagatg cggacaattc ttcattagga gactggttcg agtatgaagg
     4081 caagttgaca aatggattac gtgaaatagc agattatgct catagcaaag gattgaaatt
     4141 tggtttatgg tttgaacctg aaatgatctc ggttgattct gaactgtatc gaacacatcc
     4201 tgattttctt atgcaggagc caggtagaat gccttctgct tccagatcac aacatgtact
     4261 ggattttaca aggttagatg ttcggcagac gattgagaaa caaatgagaa agattttaga
     4321 tacgattcct cttgactaca tcaaatggga tatgaaccgt tcgctttcag atgtctattc
     4381 gattaccctt gacccacaga gacaaggaga agtagcgcat cgttatatgc tcgggcttta
     4441 tgaactactt gaacatttga caactgatta tccggaaatc ttatgggaag gctgttctgg
     4501 tggaggtgga cggtttgatg ccgggtttat ttattatatg ccgcagtcat ggacaagtga
     4561 taacacggat gcagtggagc gtatgaaaat ccaatatgga acaagccttg cttatccgat
     4621 ttcgtccatc acagctcatg tatcagctgt accaaatcat cagaccgaca gatctacttc
     4681 gttgaaaaca agaggagaaa cagctatgag cgctgttttt ggttatgaac tggatttgac
     4741 aaagctgtct cctgaagaaa aaaagcaagt aaaagaccaa attatatcct atcaaacgat
     4801 tcgtccggtg atccagtatg gccattatta tcgtttggcg agtccttttg aagaaaatat
     4861 tgccgcatgg atgtttgtgt ctcctaaaca agatgaagct attgtgtttt tagggaggat
     4921 actagcttca gctcaaccag cgttccatga agtatatctg atggggttag atgatgaggc
     4981 actttatcag gaacagacct cgaagcggat attttcgggg gccgaattga tgacagttgg
     5041 actttacttc cccgattttc aaggtgattt ccaaacagaa ctgcttcatt tcaaaaagtt
     5101 atgagagaga aggaaaaaag tatgaaagca aatataaaat ggttagatga tccacaagtt
     5161 tttagagtga accaatcaaa tgcacatagc gatcatgttt gttatgcaac aagggaagaa
     5221 gtagaaacca catcctccct taaacagtct ttaaatggat catggcgttt ttgttattcg
     5281 ccaaatcctt cggtacgacc aaaaacattt tataaaaaag attttgatgc ctgttctttt
     5341 gatgaaatca ctgttcctag tcatattgaa ttagcaaatt atgacaagat ccattacatc
     5401 aatacgatgt atccgtggga gggacatgta tttcgccgtc cccctttcac gacaagccct
     5461 gagaaagagg cggaagggat cttttcagct gctaaagaca atccggttgg ttcgtatttg
     5521 acgacttttc gattagaaga aggtctaaag ggaaaacaaa tctcgattca ttttgctggt
     5581 gtagaacaag caatgtttat ttggttgaat ggtcatttta tcgggtatgc cgaagattcg
     5641 ttcactccgt ctgaatttga tttaacacct tatttggaag aagaaaatat tctagctgtg
     5701 gaggtttaca aaagaagtac agctgcattc ttggaagatc aggacttttt ccgatttttt
     5761 gggatcttca gagatgtctt cttagttgct cgaggacaaa accatttaga agatctttgg
     5821 atcaaacctc gcttagatga caaatgtcaa aatggcaaac ttgaagtaga tttgtctttt
     5881 gagcaagtag ccaaaaacag tcagctgaat gtagcgatac tagatcctga tgggaaggaa
     5941 atctatcaac agcaatatcc gttaaaagag caaatgacga ttcgcatcga tggatttgaa
     6001 aatgtggcac tgtgggggca ttttcagccc aatttatata agcttgaatt ggagacgata
     6061 gacgaagaaa aaagagtaac ggagtatatt gactattcct tcggttttcg taaaatcgaa
     6121 atcaaagata aaatcatcta cttcaatgga aaacggatga ttatcaacgg ggtaaatcga
     6181 catgagtggc atccgaaaaa aggacgcgcc atcactatag aagatatgga aaaagatttg
     6241 gctatcataa aaaatgcaaa gatcaacggc gtacggacat ctcattaccc caaccagatc
     6301 acttggtatt atttatgcga ccaagcagga atccatgtga tggcggaaac gaacctcgaa
     6361 tcccatggct catggcaaaa aatgggcgca tttgagcctt cttacaacgt accaggtgca
     6421 gtacctcagt ggcaggcggc tgttcttgat cgagcaaaaa cgaatttcga aacattaaaa
     6481 aaccatccaa gtattctttt ttggtctctt ggaaatgaat cttatgcgga agaaaacatt
     6541 cgacaaatga acgattactt caaaaaagta gaccccgatc gacttgtcca ttatgaaggg
     6601 aattttccaa atccagcatt cgaagattcg atttcagatg taaaaagctg catgtatgcg
     6661 ccaccagaag agatcattga ctaccttgaa aataaagcgg aaaaaccgtt catcttatgt
     6721 gaatatatgc atgacatggg gaattcgtta ggtggaatgg attcttacat gacgttgctt
     6781 gatcgttatg aaatgtacca aggaggctat atatgggatt ttaaagatca ggcgatccaa
     6841 gtgactgatc caatcacagg cagagaagtg atgcgctatg gcggtgactt tgacgataaa
     6901 ccaagtgact atgaattctc agggaatggg atcgtttttg cagatggaca agaaaaaccc
     6961 gccatgcagg aggtaagata ttattatgaa aaatacagta aataaaagtc atatggatac
     7021 ggaaaaagtt gcaatcgtct tcggcgactg tacattaggt gtcaaatcgg ggaatacgca
     7081 ttatattttt tcttatacaa gaggcggact ggaatcgctc aataaaaacg gaaaagaatg
     7141 gctataccgg gaaacacttc cagttttttg gcgagcaacg accgataatg acagaggtag
     7201 cggatttgga tttaaatccg gtatgtggtt aggagccgga ttgtatccaa aagtcaaaga
     7261 aatagaagta ctggtggaca ataaaaagat cgatttgccg attgctccag taaataatca
     7321 atattcaagt aatgaatacg ctgaaacggt tgaaatcatt tttacactgg aaatcaacac
     7381 agtaccaaaa acccaagtga caatttccta ccaaatcgct ggagagggta aaatgcttgt
     7441 caatatgcat tattatggac aaaaaggatt accggaatta cccgcattgg gtttaaggtt
     7501 cattatgcca acgaaagcaa aattttttga gtatgaaggc ctatcagggg aaacatatcc
     7561 ggatcggatg gctggtggtg tcgaaggtat ttataaagtg gcaggactcc ccgtttcccc
     7621 ataccttgtt ccccaagaat gcggcatgca tatggaaacg aaatgggtag aactaacaag
     7681 aaatcaaacg ttggataata cggataaaga tcaagaagat ttttcgttgc gatttgaaca
     7741 gcaggatacg ccatttgctt tttccgcttt accattcaaa gcagaagaac tggaacaggc
     7801 aacacatata gaagaactac cgccagaaag acgaacggtt cttacgattt ttgcaaaagt
     7861 aagaggagtc gggggtatca atagctgggg agcggatgtt gagtcacctt accgaatcag
     7921 cagcgaagag gaccatgaaa tcggctttat tattcagtag caatctttag attattattt
     7981 gaaaaacgga gtttacaaga caaagctgct ttattctata tgtcatgaac aagagagtaa
     8041 aaaagttctc agatactttt gttcatgact attttcttca ataaaa
//
LOCUS       DAYYCY010000113         8040 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000113 DAYYCY010000000
VERSION     DAYYCY010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8040
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.113"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(113..569)
                     /locus_tag="ACHAZB_002051"
                     /pseudo
     CDS             complement(113..569)
                     /locus_tag="ACHAZB_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035543.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            734..3096
                     /locus_tag="ACHAZB_002052"
                     /pseudo
     CDS             734..3096
                     /locus_tag="ACHAZB_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase"
     gene            3464..4387
                     /gene="truB"
                     /locus_tag="ACHAZB_002053"
     CDS             3464..4387
                     /gene="truB"
                     /locus_tag="ACHAZB_002053"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFM8614690.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            4391..5332
                     /gene="ribF"
                     /locus_tag="ACHAZB_002054"
     CDS             4391..5332
                     /gene="ribF"
                     /locus_tag="ACHAZB_002054"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HFM8614691.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            5684..6469
                     /locus_tag="ACHAZB_002055"
     CDS             5684..6469
                     /locus_tag="ACHAZB_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM8614692.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            6482..7333
                     /locus_tag="ACHAZB_002056"
     CDS             6482..7333
                     /locus_tag="ACHAZB_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFM8614693.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(7457..7909)
                     /locus_tag="ACHAZB_002057"
     CDS             complement(7457..7909)
                     /locus_tag="ACHAZB_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8614694.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
BASE COUNT         2704 a         1370 c         1701 g         2265 t
ORIGIN      
        1 tttttgtttt cattaagacg tattacctaa gagttccatg actttagtct tgcggcgtgt
       61 cagctaacca aaaaagagaa aaagaaaagt aaaaaattcg actttctttt ttctagtagt
      121 atataaattt ttgcatatag ataaagatca agataccgat aacggaagct agattgtaat
      181 ctattttagt gacagtcagg tatagccaag taactagaag atcagtggaa cctgcaccat
      241 aaaagtttga gtttgtcggt ccgttgacag ttaaaagaaa aatcacgttg aagttattga
      301 tatttccgat tacgccgctt cgatttggtc acgatccaaa ttttggataa tccctgtaga
      361 aattagcata gtagctggga taccaattca catattgaca aagataacag agacttttac
      421 ataaaacgga tcaatcagga aaggaattgc tcgatccagc cccagttttc cagcaatgtg
      481 ttaactggcc cagcgtcatt cagcaaatta tgcatgatga ggagcgaaat gaactgcgga
      541 acagccattg tgataacgac aagttttttg gatcaatgta aaaaacgtca gcgaaaggaa
      601 aaatttcgtg gttttttata cctgttcatt tggctagata taaaatccaa acaaatgaac
      661 agaagtgctt attaggtttc aattgggagt atctgcgcta gaataagagt aataatgaaa
      721 aggagtgtta tgtatggatt attctagtaa agaatacttt gataaaatga cggcttggtg
      781 gcgtgctgct aattatcttt ctgttgggca actttatctt aaagataacc ccttactgag
      841 acgtacactg aaacccgaag atgtcaaaaa acatccaatc ggtcattggg gaacgattcc
      901 tggccaaaat tttatttatg tccatttgaa ccgagtcatc aataaatatg atttgaacat
      961 gttttatatt gaagggccag gacacggcgg acaagtcatg gtatcgaatg cttatttaga
     1021 tggaagttac acagagatct atccagaagt aacggaagat gagacaggaa tgcaaaaatt
     1081 attcaaacga ttttcatttc ctggaggcat tgcttctcat gcagcaccag aaacaccagg
     1141 ttctatccat gaaggcggag agcttggata ctctctttca catgcagtcg gagctgtatt
     1201 agacaatcct gaagtaatct ctgctgtcgt gattggtgat ggtgaagcag aaaccggtcc
     1261 tttagctgga agctggttct caaatgtctt tatcaatcca gtgatagatg gagctgtttt
     1321 accgatcctt catttaaatg gtgcaaaaat agccaaccca acgatcttgg cacgtaaatc
     1381 agatggagaa ttagcaaatt actttaacgg acttggctgg gaacctttct ttattgaagg
     1441 aaatgattca gaaaaattaa atcctgttat ggctgaaaaa atggatcaag caatcgagaa
     1501 aattaaatcc atccaaaaag aagctcgcct gaagacagca acagatgtcg tgatgccaaa
     1561 atggccagta ttgatcgtac gcacaccaaa aggatggacc ggtccaaaag aatgggacgg
     1621 ggaaccaatt gaaggaacct tccgagcaca ccaagtaccg attcctgtag atcaagaaca
     1681 tatggatcat gcagatgctt tactccgttg gctgaaatcc tatgaaccag aaaaattatt
     1741 tgatgcacaa ggacgtattt tagaagaaat cagagaaatc gcgcctactg gtgaccaaag
     1801 aatggcaaaa aatcctatca ccaatggtgg aatcgatcca agaccgctta ttatgccaga
     1861 ttggaaaaaa tatactttgc aatttgaaaa accaggctcc gttaaagcag aagatatgac
     1921 cgaacttggt aaattcgttc gcgagatcat tgagaaaaat ccagaaaatt tccgtatatt
     1981 tggaccagat gaaacaaaat caaaccgatt gaatcaagtt ttcaaaacga caaatcgcca
     2041 atggatggaa aaaatcgaac cagaaaatga tgaatggctt tcgccatctg gtcgcgtcat
     2101 cgacagtcaa ttatcggaac accaagatga agggttctta gaagggtacg ttctgactgg
     2161 acggcatggt tttttgcaag ttacgaatca ttcttgcgag tggttgactc tatgttgact
     2221 cagcacttca aatggatgcg gaaatcacat gacctttctt ggcgaaatga ttatccttcg
     2281 ctaaatctca ttgcaagctc aacagtcttc cagcaagatc acaatggata tagccaccaa
     2341 gatccaggaa tattgacaca tttggcagaa aagaaggcgg agttcatccg tgaatatctg
     2401 cctgcggatg caaatacatt gctcgcagtc atggataaag cattccgctc gtcagaaaaa
     2461 atcaatttga tcatctcttc caaacatccg cgtgctcaat tttactcagc agaagaagca
     2521 gccgtcttag tgaatgaagg cttgaaaatc attgattggg cttcgacagc aaaagaggag
     2581 gaacctgaat tggtcattgc tgctgcagga acggaatcta acttagaagc attagcagct
     2641 gtaactttgc tgctggaaga attcccaaaa ctaaaaattc ggttcatcaa tgtcgtagat
     2701 ttattgaaat tacgtcatcc aagtcaagat ccgcgtgggt tgagcgatga agaatttgat
     2761 caatatttta caaaagacaa accgatctta tttgctttcc acggttatga aacattggtc
     2821 cgcacgattt tctttgatcg tcataaccat catttaatga ttcatggcta caaagaaaat
     2881 ggagatatca cgacaccatt tgacatgcgt gtagtaaatg aacttgatcg ttatcatttg
     2941 gcaaaagacg cagctttaaa aatcaaagga agtcaagcag aagattttgc gaaaaaaatg
     3001 gatcagaaat tacaagaaca tcaaaactat attcgtgaaa atggtattga tctgccagaa
     3061 gtattggatt ggaaatggaa aaatcttgac caataaaatg ataaaatcga tcccgcaaaa
     3121 tagaattttt tacattgatt aagaaactga aaaaataaat aaaaaaaacg gaaaaaagac
     3181 tggaagaatg gccagtcttt tttttcgttt ttgggaagtg aaaaccaaac tatttacctg
     3241 taagccaccc ataaatccaa tcaaatggtg caaagaaaaa atcttccatt ctattccctc
     3301 ctttaaaaaa gcataacatg atttctattt acgtgagcat gaattttgtt acgtttttat
     3361 tgccgaaaaa taaaaaccgt tcattatgat gtcaaattcg tcagtaattg atgcaatgtg
     3421 ttataataat tcagttaaat caaaaatgaa atggagaaaa gacatggacg ggttattacc
     3481 tttatggaaa gaacgcggaa tgacaagtca tgattgcgta ttcaaattaa gaaaaatctt
     3541 acatacgaaa aaaatcggac atgggggtac gcttgaccca gacgtagacg gtgtgttgcc
     3601 catttgtatt ggaaaagcca caaaagttat cgaatatttg acagattctg ggaaaatcta
     3661 tgtaggcgag ataactctag gtttcagtac aacgacggaa gatcgatccg gagaacgggt
     3721 agaaacaaaa ccagtagata tgtctttatc tgaaaaaaaa atagatgaag tgatggcttc
     3781 atttattggt gagatcaccc agataccacc aatgtactca gcggtaaaag tcaacggtaa
     3841 gcggttatat gaatatgccc gcaataatga aacagtggag cgtcctgtta gaaaggctca
     3901 gatttaccgt tttatccgga caagtgaatt gacaagagac gaagtgacag gaaccatttc
     3961 ttggcgcttc gaagtagaat gcgggaaagg aacctatgtc cgcacattag ctgtagatac
     4021 aggcaagaaa cttggttacc ctgcgcacat gtctgatctg acccgaacag caagtgccgg
     4081 tatgtcccag aaggatgctc tgacattaga agaagtagct caagctgtga aaaatgggac
     4141 aatcgatgat tgtttgctgc caatagaaaa aggagtggaa cggttcaaac gagtagatat
     4201 tactgatgaa gtatggcaga aagtaaaaaa cggtatgaga cttgattata ctgtatttgc
     4261 gctcccagaa atgccgtcag aagaaattgc tttgttttat aaaggaaaag ttgtcagtat
     4321 ttatcagcca aacccgaaag aaaaaaacaa attaaaacct agtaaagtat tgagaaatga
     4381 ggaataacga atgaatatca tcaagatcag acatccttac caatcagaac aaattccaga
     4441 agacgatgtc gttttagtgc tgggattttt cgacggcgtg catatcggac accagaaagt
     4501 cataaagaca ggtatggaaa ttgcacaaaa agaaggattg aaattagcat tgatgacttt
     4561 caatcagcat ccatctattg tctttaaaaa gatcgatcct agcagcgtga aatatttgac
     4621 tagccttaag caaaaagaag aaaaaatggc cgctttgggt atagattatt tatatgaagt
     4681 tgattttact tcttcttttg ctcatttagc accgcaggaa tttgttgatc aatacatcgt
     4741 agggttacat gcaaaatatg ctgtttccgg ttttgattat acgtatggac caaaagacat
     4801 tgcagatgtc gctcattttc ctgaatatgc caaaggaaga ttcaaaatcg tcacagtttc
     4861 taaagaagaa gaacaaggtg aaaaaatcag ctctacccgt attcgtggat tactagacag
     4921 cggacaagta aaagaagcag caaaactatt aggttgcaca tatgaggtag aaggggtagt
     4981 tgtccatggc gatgcacgag gcagactact aggattccct acagcaaatg tcaaagtaaa
     5041 gagcacggtt catttgccta aagaaggtgt ttatgtgtcg gaaataaaaa ttggggaaac
     5101 atggtatcca gccatgggtt caattggaca taatgataca ttcggtgaag gacgggagct
     5161 gactgtagag ttgtatattt tggactttaa tcaagatatc tatggtgaac atgtcgcggt
     5221 acgctggaat caatttttaa gagatcaagt gaaattttct agtgtagagg aattgattga
     5281 acaactcaag gaggatgaac aagcaacagc tgaatatttt tctcagcaat aaaacattat
     5341 gcataaaatc tcttgtttat gtataactag caattacaat aaaatgggtg ataaataagc
     5401 ccaggaaagg ggctgtgaca aaagtcttgt cacagccttt ttttccaaat aaatggtttt
     5461 tcagaagtag cttcttcgga aataagccga aatttcttga agctgaccac ttctgtcaca
     5521 acctctttct ttatagaaat aaaaggacac tagcaattca tgaaaacgat tgctagagcg
     5581 tatcatttgc tttagactat atatcttggt gtacactcac tttaaggcgg aaataaaagt
     5641 tatccgtaat aacaggttta ggtatgaaaa aggagtgggt actatgtaca aggaattaga
     5701 aaacaaagtc gcagtagtaa ctggtggctc aaaggggatt ggaacagcga ttgctcaacg
     5761 attcgggaaa gaaaaaatga atgtagttgt caactataac tcggataaag aaggagcaga
     5821 aaaagcagtc aaagcaatcg aagaggcagg cggaaaagca gtctctgtac aagcagatgt
     5881 cagttctgaa gaaggaatac agcgtttact tgatacggct ttagaaaatt ttgggacact
     5941 ggacgtttgg gttaataatg ctgggatgga aaatcagcat ccaacccatg aacttagcct
     6001 tgaagattgg gaaagagtgt taaaagtcaa tttgactggc gtttttatcg gaacgaaagc
     6061 ggctttaaat tactttacag agaataataa aaaaggaaat atcatcaata tgtcttctgt
     6121 tcatgaacag atcccatggc caacgtttgc tcattacgct gcttcaaaag gcggggtaaa
     6181 attatttacc gaaacggtag caatggagta tgcaccgaag ggaatcagaa tcaatagtat
     6241 aggccctggt gctatccgta cacctattaa tgcggcaaaa tttgatgatc ctgaacaaaa
     6301 agaactcttg gaaagtatga tcccactagg acgcgtaggt gaaccaaaag aaatagcggc
     6361 gacagctgca tggttggctt cagacgaatc cagttatgtt acaggtatta cactatttgc
     6421 agatggtggg atgactttat atccttcttt ccaaggtgga aaaggataag ggtgaataaa
     6481 aatgggttta ttgattgcat tgatcccggc agttggctgg gggatccagc cactgatttt
     6541 gaaaaagatt ggtggcagac caacaaatga gatattagga actggtattg gagcggtgct
     6601 tgtcggctta ctcgttcaat tattcgcttc tccagctggt atttcagtga ctacattttt
     6661 gattagttta ctttctggtg cattttgggt tattggacaa acaggacagt atacatcttt
     6721 caacttgatt ggtgtttcta aaacgatgcc aatttcgact gcattacagt tagtcggaac
     6781 ctcgttgatt ggtgtcttcg cttttggaga atggtctgga agtatgggga aaatcattgg
     6841 agcagcagca atcattcttc ttgtatttgg ttctgcttta acggcaatca gtgatgatcc
     6901 tggaaaaaaa ggcggtatca caaaaggaat tagtctatta gcatcaacaa gtattggtta
     6961 ttgggtatac agtgcattgc ctaaactagt tgatgcaagc ggactttcca ttttctttcc
     7021 gcaaatgatc ggtgtctttt taggggcagt gatttatgtg attttccgta aaccaaccgc
     7081 attttctgaa gacaagagtt ggaaagcaac gatcgttgga gccatcttta gtgtgtcagc
     7141 attggcttat attttttcag caaatgaaaa tggggttgct actgcatata tcattacaca
     7201 gctaaacgta gttatctcta cattaggcgg tatgtacatc ctgcatgaaa ataagacacc
     7261 aaaagaaaaa aaattaacga ttatcggatt attgctgatc gtagtaggaa gcatggtcac
     7321 ggtctttatt tgaggataaa gatggtaaaa tttcatttat taaaaaaata tttgtttgaa
     7381 ccaaacagta agtacttata aaaaaatagc gctagaattt ccaacatctc tggaatttct
     7441 agcgcttttt aattgtttaa gatacaaggg aaatatctgt ctggatcaat aatttttcat
     7501 tgatccattg actaaagttt acgacataat tgaagttacc accgattagt atctccgcat
     7561 gattgtcttt ataaagatcg tattgtaaaa gacggtcttt tttatttgcg taaacatcgt
     7621 tatggtactg gactaattgt tctcggaatt gattgattgc tgtaaaatca cgatattgaa
     7681 ggttgaaacg catcgtgatt ggtgcattta gcatgaatcg ctgcatttct tttgctgctt
     7741 cctctgcttg atctccgtat aggaactcaa aaagattacg ttcagaaaat ttccattgcc
     7801 cgccaacctt tgttcctttt agttttcctt catgaagata atttctgata gaacgtgtag
     7861 taacacctaa ttgttcggct aattcattta ctgagtaaaa atctttcaat gttcaccctc
     7921 ctatgtgaat ggttctactg acttagttga tatttgctta atcacgaaat gtagaaagaa
     7981 cgattttaat taaggtcatt gtaaaaatca agtcagaaaa ttctaacttt tatttctcta
//
LOCUS       DAYYCY010000114         7890 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000114 DAYYCY010000000
VERSION     DAYYCY010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.114"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..148
                     /locus_tag="ACHAZB_002058"
     CDS             <1..148
                     /locus_tag="ACHAZB_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8614695.1"
                     /translation="FAKNQGYNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYM
                     KKTL"
     gene            complement(226..759)
                     /locus_tag="ACHAZB_002059"
     CDS             complement(226..759)
                     /locus_tag="ACHAZB_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HFM8614696.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(853..2031)
                     /gene="mutY"
                     /locus_tag="ACHAZB_002060"
     CDS             complement(853..2031)
                     /gene="mutY"
                     /locus_tag="ACHAZB_002060"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFM8614697.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(2024..2821)
                     /gene="recX"
                     /locus_tag="ACHAZB_002061"
     CDS             complement(2024..2821)
                     /gene="recX"
                     /locus_tag="ACHAZB_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFM8614698.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            2928..4310
                     /gene="rlmD"
                     /locus_tag="ACHAZB_002062"
     CDS             2928..4310
                     /gene="rlmD"
                     /locus_tag="ACHAZB_002062"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFM8614699.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(4484..5062)
                     /locus_tag="ACHAZB_002063"
     CDS             complement(4484..5062)
                     /locus_tag="ACHAZB_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HFM8614700.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            5384..5854
                     /locus_tag="ACHAZB_002064"
     CDS             5384..5854
                     /locus_tag="ACHAZB_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HFM8614701.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLTMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(5962..7188)
                     /locus_tag="ACHAZB_002065"
     CDS             complement(5962..7188)
                     /locus_tag="ACHAZB_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFM8614702.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
BASE COUNT         2303 a         1575 c         1326 g         2686 t
ORIGIN      
        1 ctttgctaag aatcaaggct ataatacgat tttattaggg gcacagtcca cagctgaaac
       61 tttttacgaa aaactaggat atacagctta cggcgatcct tttgaagatg ctggcatgcc
      121 tcatatttat atgaaaaaga ctttataaaa atttatttcg aaaaaagcaa aaaacagcaa
      181 gcttttgaga aaaatctctc actggcttac tgttcttttt tgtgtttact tacgcgacaa
      241 ttgcttgtaa cggttatacc aaatatcaat atagccttcc gagaacgggc catctccatt
      301 attgatccag tcaaccagaa tcttcacatt ttcttttagg atgaagtcaa tatcgttcgg
      361 gtaattcatc attttgctgt gcatctcata ttcatcgaca tccaatagtc gtttctcgcc
      421 gtcaggaaat accttgatat ccaagtcata gtcgatatat ttcaaggcct catcgtccag
      481 caaaaaaggt gaagctagat tacaataata ggaaaccccc ttttctctga tcattgctat
      541 tacattgaac cagtattttt tatgaaaata aacaatcgct ggttcgcgcg ttacccatcg
      601 gcgtccatca gattcagtga ctaatgtgtg atcattcacg ccgatcatag agtgttcgct
      661 tgtttttaat accatggtat ctcgccacgt tcgatgcaat ttgccgtcgt gcttgtaact
      721 ttggatcgtc acaaactctc cttcttttgg aactcccata gcaacccttc cttaagcagc
      781 attttgtgct ttgtgtttgg gtgttcatta caccgacatc ataattatta tatcaataaa
      841 aatgagcttt gcctagaaag gattcctatt gtttcgattt ttctctaact gatctaccag
      901 tttcatttgg accttaggaa aaacgaccga atcaaatgca tcaggcttca accacttcga
      961 agttttgttc tcggtaaatt cttgttctgc tccctcggtt gcccgtccgt aaaaaaggag
     1021 aacatgccat ttcagatgac taaaaatatg agtaacttct cctaaatgtc gtgtctgcca
     1081 aacaaagagt tcctgctttt caaatgggag attttgctct tcttctgcta ctaggtcatc
     1141 aaacagatcg atttctggct ttgtttccca ttcttttttc aatcgctcat actcctcttg
     1201 tgtcacttcc atcattggaa atgtccacat attcgccagc aatttttggc tatctctttc
     1261 ttcaaaataa tacgctccag aatgattttg aagagctgca ctgatataat aaacgtcttt
     1321 tggctttgct ttttttgttt ttacaggaaa actcgtttgg attcctcgtt tgttagctaa
     1381 acaaaaggct tggatcggac aagtctcaca ctttggagaa gttggtgtac agatagctga
     1441 acctagatcc atcattgctt ggttgaattc acctggatgc ttttcatcaa tgattttccg
     1501 catcgcttca tcaaagattt tcctgctaga agcttttgcg atgtctgctt caatgcaaaa
     1561 caatctgctg actactcgca tcacgttgcc gtcaactgca ggttctggaa gtccaaatgc
     1621 gatacttgca atcgcgcctg tcgtatatgg tcctattccc ttcaatgagc taatttcttc
     1681 aggcgtttgc ggcatttccc catcaaattc tgacatgatc tgtttagcag cagcttggat
     1741 gttccgggct cttgagtaat aaccaaggcc ctcccatgct tttaaaagct tttcttctgg
     1801 agcatttgct agctcttcaa ttgtaggaaa ccattccatg aaacgataaa aataatcgat
     1861 cactgtgtct actctggttt gctgaagcat gatctcagag atccagatac gataaggatc
     1921 acgattatag cgccaaggca ggtttctttt ttcttgttca taccattgaa tgaactgatc
     1981 ttgaaattct tttgtttctt tatctgtcca tttttcccat tctttattca ctttcctctt
     2041 ccttttccgt aatgaatcgc tggatcatat ccaaatcaaa tcctttttga tataagcttt
     2101 gctttacttt tagatttctt tttttaggtt caaaccttaa atttcttcgc catagtttat
     2161 ctccttgttt gactaaataa tcatattcta tttcttgatc tgcttcttgc ggaagatcag
     2221 ctatcgcttg ttggatcact tcttgagaaa agcctttcgt catcaatgtt tgttggattt
     2281 ttctcaataa ttctttttgg ctttttccgt gattttttcc aaacgctttt tcggctgtct
     2341 gatgtgcatt ttgaacttgg tcttcaaatg tatattggct gagcgcttgt tcaatgattt
     2401 caggtttcaa acctttttgc tgcatctgct gcgctaattt ctttggtcct ttgtcactta
     2461 agcgcatatt cgtgcgcaca taactttccc catagaccaa atcatcgaca aggtccaatt
     2521 cttttaagcg ggctacgatt ttatttcgat cttcttgaag gatttccttt tcttttaagt
     2581 atgttcggat ttctttttcc gatcgcaact gataacttaa gtaattcatt gctaggtgaa
     2641 aaccgaaatc agctccgcta ctcttcttga tctcttggat agtttcttcg tctaattccc
     2701 gtccttttaa taatcgatag cgtactaata catcttcaga tacttgtaaa gcttctcctt
     2761 cagataaatc tacgcgatag aaagggcctt tgcctttact gattctcgta acggttaaca
     2821 taaacatcct cctcgaacat atattctcta tcataccaca gaaaatcccc gtaatcaggt
     2881 attatatgtt agaataaacc acagaaaaaa gaaaaaggga gatacttatg gcagaaacaa
     2941 ttgtcaaaac tggacaaacg gttcctttga aaatcaagcg tttaggcata aatggtgaag
     3001 gaatcggtta tttcaaacgg ttgatcatat ttgttccata tgcactacca aaagaagaag
     3061 tactagtgaa aatcacgaaa gcaactcctc gctatgcaga aggagaatta ataaaagtca
     3121 aaaaaacaag caaagatcgc gtagtggcac catgtcctgt ttactatgaa tgcggtggct
     3181 gtcaattaca acacttagct tatcaagctc aattggattt taaaaaggat ctgcttttgc
     3241 aagcattgga aaaattcaaa ccagcaggct ttcgatctta tcaacttttg ccgacgatcg
     3301 ggatggatga gccttggcat tatagaaaca aagcacaatt tcagttacga aaaaataagc
     3361 agactcaaaa aattgaagct ggtttatacg aagcaaactc tcatcagtta gtccctataa
     3421 cagactgctt ggttcaagaa cctcgaacac aaaaagtaat gaatactgtt gttcaactat
     3481 tgaataaatt tgatgcaccc atctatgatg aacggacaaa cagtggaatc ttccgcacaa
     3541 tcatggttag aatcggtgtt aaaactgggg agttacaagt tgtttttatt actcgcagta
     3601 aaaaattccc gcaaaaaaac gcaatgatcc gtgagatcaa tgaacgactg cccgaagttg
     3661 tttcaatcat gcaaaatgtc caggcacaaa aaacgtctct cgtgatgggg gatgaaacga
     3721 tccacttatg gggaaaagaa gccattgaag aacaaatcaa tgaagtgact ttcgatcttt
     3781 ctcctcgtgc cttctttcaa ctcaatcctc agcagaccga attgctttat ggcgaaggaa
     3841 tcaaagcatt agatgtccag cctaatgaaa cgatcgtgga tgcttattgc ggagttggga
     3901 cgatcggctt aagtgtagca aaacaagcca aagaagtacg gggaatggat accgttcctc
     3961 aagcaatcgc tgatgcaaaa aaaaatgcgg aaagattagg atatcacaac actcgatatg
     4021 aagtcgggac agcagagcaa gttctgccta aatggttgaa tgaaggtttt cagcctgatg
     4081 gtatcatcgt agatccacca cgtacgggtt tggataacgc attgatccaa tcattattga
     4141 aataccctcc aaaaaaactt gtttatatct cctgtaatgt ttctacactt gccagagatc
     4201 tggttaattt aagtaaaaag ttcaagattg aatatttaca gtccgtcgac atgtttcctc
     4261 agactgcacg ttgtgaagtt gtcgtgaaga tgacgagact cgctgactaa atgaaagagg
     4321 ttgtgaaaaa gtctgccctg tatcaagtaa taagaacagc caaacaaaaa tagctcttca
     4381 tgttttatga atactgtact tgtgaccgaa gagttaacag gaggacacta tttatttaaa
     4441 aaaagggttg tgacatagtt tttgtcacag ccctttttga ttattattgg atattcacgt
     4501 ctatcacttg ataaaatgca tttgtcgtat cgctgatatt ccatactgct aaaatcacat
     4561 tatatccttt tcgatcctgc ggaagtgtga tcgtttggtt gactgttttt ggtggaacag
     4621 ttgctttgtc gtctattttt gtgatcaatt caaaatcact aaatttcaaa ggttgattag
     4681 gattccaagt tggtttggta atgtagtaat cccaagtact tgtccgatgt tgtgctgtta
     4741 aattccaagt aatatttaat gttcctggat taaccatgtt tttatgccat ctgttagatg
     4801 tctgttcatc taaaggctca aagccgctga ctcctgcact agcaattttt ccatcaatga
     4861 atgtattttt ggtagcttcg atactttgcg gttcccattg tgctcttccg acattttgat
     4921 ttaagtttcc tgctgctgat gttcctaggt atgcacggct tcctggagaa gtaatataac
     4981 catgcgcaaa acctggaatg ctaaaaccga acaaccctaa actaactaac ccaataaaca
     5041 ataacgtact aatctttttc atgtagaaac ctccttcatt tgataaccac attgtaaacg
     5101 ctttattgta ttaacacaaa cattatgctt ttataatttt atgttgaagt aaaaaaggtg
     5161 aaaaaagtaa gatgcagatt ataacagaag tagacgagaa aagtacaaat agaaaacatg
     5221 agaatcaaat catatgatct ttttcaaaaa catcattcat ataaacaaat gttcaacttt
     5281 ttaataacaa caattatcaa tttttttctt acttcacaaa ctaaatacag gtcttgctgt
     5341 gagccttcat ggtaaactaa tattatagaa aggagttagt aaaatgaaga tgataaaaga
     5401 tattcgtttt tgggtttgtg ttataggaat tgtcatatta ggctttttat cagggttgct
     5461 tagcggaaat cccggaaaat attactattc tttgcaactt ccaccatttg caccacctag
     5521 ctggatattt gggccgatgt ggaccttact gtatatattg atgggtattt ctctttattt
     5581 gttacttaat cacaacaaca aaaaacagcg aaacaatctt gtcggtcttt ttgtgattca
     5641 gttcattttt aattttatat ggtctgctct attttttaat ttaagaaata ttttcattgc
     5701 agcaatcgat attacacttt tagttatttt cttgtcagtg cttatgtatc agttgtggct
     5761 gcatcatcgc ctgacaatgt ggctcatgat tccttattat ttatgggtac tattcgcaac
     5821 tctattgaat tattcaattt attttttaaa ctaagcgcta aaaagagacg tcctgtaatt
     5881 taacacatag aaaaagtgcc acctaagtta gattttttgt ctaatttagg tggcactctt
     5941 tattaaccaa actatttttt tctagtaccc acttgttgtt ccatactcat tattctggtc
     6001 ataaccataa ccattttgtt cctgtccagc gtaaggatca taactttgag tatcttgact
     6061 tggtgtatag tactgacttg tatcttctgt ttgctgataa taggtattcg gatcttggta
     6121 ctgatcatat gtgttttctg aataggaaga ataatcggtg tacccttcac tattcgatcc
     6181 atcaccgttt gtcgaatcgc catcttccga atcatcaaag aacatcattg aagcattctc
     6241 atttttcaaa ttaggtaatg tatcgcttgt ttttatattt aattgtttct tcagttcatt
     6301 ttggatcgaa agaagatcat tttttcctaa aatttgatag tagatatcat tgatcatttg
     6361 tccttcgcct tgcaactggt cttgtttgat tgtttcaaac gccggggtgt agttcgtcgc
     6421 tacatcaagc atgtcatccc aatctaagtc cgtcgtcaca ttgttctcaa ctgcttttaa
     6481 aatttttcta taattgctta cgccatctaa acttaaaact ttattgacga ttttcgtcac
     6541 tacttcccgc tgacgacgtt gtcgaccgat atcgccttct ggatcttcgt gtcgcattcg
     6601 agaataggct aaaccttttt ctccattcaa tgtttgttca cctactggta catggacacc
     6661 atccaaagta aagtcaattt tattattcac tttgatgcca ccgaccgcat tgatcaaatc
     6721 ttccatgcca tccaaattga tcgttacata atgatcaata ggaatatcga gcagttgctc
     6781 aattgaatcc atggccattt cgacaccacc aaatgcgtag gcatgattta atttatctac
     6841 cgttccataa cctacgatat tcgtataaat atctcggtcc aaactgatga tcgttgattt
     6901 tttttgtttc ggattaactg ttactaccat catcgtatca gaacgtcctt gttccgttct
     6961 tcctaatcca ccggtatcta aacctaatag caaaaccgaa aaaggttctt tgtcatcaat
     7021 gcttactctc tcagaacgtt tggaggatat tcgattagag tttttagaaa ttttgtttac
     7081 tgtttggctc gcttctccca taaatctgat tccgtatgct gataatccgg caatcagcaa
     7141 aacgaatacc cctaacaagg ttaatagaac tttttgccat cgcttcataa tacacctctg
     7201 tttcacattt tctaaaatat tgtctcatat aactgttcaa tcagcaaatt cactttttaa
     7261 tcataagaaa actttaaaaa acatttttgt gtaacgatta ttatttttta aaatttctca
     7321 aaattcttaa ctatcatacc ataccgtgaa aaagatcgaa agaccgaata cacgcagttc
     7381 gcaatcgttt tatctttttt tatgtatttt ttccagaaaa agcgaacaac aaggtatttt
     7441 ccaaataatc gtgcagtaaa atgatcgcac atacctttcc catcttttta acagtctttg
     7501 cctaccgttc agattatcat tcgatttcac gacaatttct tcctatgact ggtccttttg
     7561 ttaaacccat cttctattcg ttttatttcc agctcactgc atggctctta aaatgactcg
     7621 aactagtgat tgcattacca tcatctttta atggatagat atgctcctct tcaaagtaaa
     7681 acgattttct tttaaagtaa tagaattgac atggtaatga gtatgtatac tagtggtttc
     7741 acaaatggtc ttttattcaa catccaaaaa ctgaaatttc tttaaaatcc attactaacg
     7801 aagaaaatgt tccttgcttg ataattagtt aggacatgtc aagtgaacaa atgcatttac
     7861 ttagccaaaa aatctaaaaa cttcataaat
//
LOCUS       DAYYCY010000115         7792 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000115 DAYYCY010000000
VERSION     DAYYCY010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7792
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.115"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            10..711
                     /locus_tag="ACHAZB_002066"
     CDS             10..711
                     /locus_tag="ACHAZB_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFM8614703.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1320..2336)
                     /gene="tsaD"
                     /locus_tag="ACHAZB_002067"
     CDS             complement(1320..2336)
                     /gene="tsaD"
                     /locus_tag="ACHAZB_002067"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFM8614704.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALAAKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2333..2797)
                     /gene="rimI"
                     /locus_tag="ACHAZB_002068"
     CDS             complement(2333..2797)
                     /gene="rimI"
                     /locus_tag="ACHAZB_002068"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFM8614705.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2804..3346)
                     /gene="rimI"
                     /locus_tag="ACHAZB_002069"
     CDS             complement(2804..3346)
                     /gene="rimI"
                     /locus_tag="ACHAZB_002069"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFM8614706.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3330..4052)
                     /gene="tsaB"
                     /locus_tag="ACHAZB_002070"
     CDS             complement(3330..4052)
                     /gene="tsaB"
                     /locus_tag="ACHAZB_002070"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFM8614707.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(4243..5223)
                     /locus_tag="ACHAZB_002071"
     CDS             complement(4243..5223)
                     /locus_tag="ACHAZB_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8614708.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(5247..6731)
                     /gene="galT"
                     /locus_tag="ACHAZB_002072"
     CDS             complement(5247..6731)
                     /gene="galT"
                     /locus_tag="ACHAZB_002072"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0017103 - UTP:galactose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019388 - galactose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HFM8614709.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6743..7732)
                     /gene="galE"
                     /locus_tag="ACHAZB_002073"
     CDS             complement(6743..7732)
                     /gene="galE"
                     /locus_tag="ACHAZB_002073"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFM8614710.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
BASE COUNT         2169 a         1690 c         1383 g         2550 t
ORIGIN      
        1 gactttttaa tgcgtgttaa taaaatcgat tttcactttt caaaactcag atatcaacct
       61 gattttgaac atttctcgga tgaagagttc caacagatca aggaacatac tgttcttcgt
      121 tcttataaaa aaggacagat ccttttcgac gatggtgatc ccagacaacg gttgtatatc
      181 ttgaccaaag gactcgttcg tttagaacgt tatgatgaaa gtgcaactta ttactactat
      241 gactatgtga atacaaataa cctttttcct atgtcaggaa tgtttggaga agagacctac
      301 acttatacag ctcaagcgat gactgatatt gaaacattct accttcctgt ttcctactat
      361 gaaaaattag cacgaaaaaa tcccgaacag ttgatctata tgatacaaaa aatttctaaa
      421 gtagttgaaa tacatgaatt gcgtatccaa atcggattga cctctagcgc ctttgaccgg
      481 gtaaaacaaa atttattcat tttgaaagaa gaattgggcg taccaacaaa agatggtcat
      541 atcaccattc cttatccgat caccttaaaa gaattagcag tgaatagcgg cacgacacgt
      601 gaaactgctg gacaagtaat caaacaacta cgtgattccg ggctgttaga ttatgacaaa
      661 aagcgtttta ctttctgtaa gacagctact actccagcta tgagagaata acgcgaacaa
      721 aaataagcag tgaccctgat aaatatcagg gtcactgctt attttcctcc ctaaaacttc
      781 tgaaagatcc ggcagtttta gatcactgga caagactcaa agattttcta tgtgtgtttc
      841 agttatgtaa tattcactgt ttgcaaaaaa tactgttaga ttaactagat gaagcgtctt
      901 gtcctcatcc gatctagatt attcgatatt cctcgacaat aaacgattac tcagtgaggg
      961 caatcagagg aacggataat gataaataag ctgtaaaaaa tagcaaaata acaataatga
     1021 ttaaaagtat gtaaaaaata gggctttgac aaaaacgtgt cacagcccta tttcgattaa
     1081 acggtgtgta cctgtcaact cttcggtcac aagtcataat gttcataaac atgaggagct
     1141 gttttttgaa attgttcctt gtgaatcaga gttaaacgac aggcttacga actcttgatt
     1201 aaacggcgtt caaaagcttg catacgcggt aataagaaca gccaggtcaa aaacatgaga
     1261 agccgttttt cctgggctgt tcttattact tgatgcaggg cagctttttc acaacctctt
     1321 tattcagata aaaccaagct tggatcagca ttcaactgat agctagcaaa atttcttttt
     1381 tgcatttcca catgtgctgc tgctccaatc attgcagcat tatctccaca aagtcgaaga
     1441 gggggaatca ctagttctac ctcagggagt ttagctgcca aagcatcctg caatccttca
     1501 cgcaaccctt gattggccgc tacacctcca gctataatca gttgcttgac tggataattt
     1561 tgacaagctc gaagtgtttt agtaatcaat acatcgatca cacttgcttg aaagctagct
     1621 gctaaatcat ttttgtctag atcttctcca cgctgttgtg cattatgcac caaattgata
     1681 aatgcacttt ttaatccgct aaaactgaaa tcataattat cttcatggat catcgctcgc
     1741 ggaaaatggt aattatcttt tccctgatga gctagctgat cgatttcttt tccgcttggg
     1801 taagaaagac ctaatactct tccaactttg tcataagctt ctcctgctgc gtcatctctt
     1861 gtctccccaa tgatttcata tgatccatct tcttgcatat agactaattc tgtatgtcct
     1921 ccgctaacaa gaagtgccat caatggaaac tggaatggtt taaccagacg ggctgcatag
     1981 atatgccctg ccatatggtt tacaggaata agcggcaatt ggtgtgccca agcaaaagcc
     2041 ttagctgcag aaataccaat cagcaacgag ccgacaagtc ctggtccata agtgacagcg
     2101 actgccgata aatcttctgc cgacacacca gcttctacca gtgcatcttc caagcataag
     2161 gtgatttgtt ctacgtgatg gcggctagct acttcaggta ctacgccacc aaaacgttta
     2221 tgactgttta tttgagaagc aacgatgtta gataagatct ctgttccatt tctaacgacc
     2281 gctacactcg tttcatcaca acttgattca atcgccagaa tcaattcttc attcatttca
     2341 tcgtctccgt ttttaacttt ctgctcataa tgatcgcatc ttccgtcgga ttgcgataat
     2401 atgatttccg tttacctaat atacgaaact tttctgattc ataaagtttt cttgctgctt
     2461 cattagatag acgtacttct aaaaaaacat gcagcgaatc ttttatttgt tccgtaatca
     2521 gtaattgtaa caaatgccgt gcatgccctt ttccttgttc tgaaactgca acagcaatat
     2581 ttgtgatctc gacttcatct attactttcg agtatccaat aaagccacag atctggttat
     2641 cttcagctaa aagcagataa tcagagtgag gctgttggat gtctgccaaa aactgcattt
     2701 tcgtccaagg tgaaccatgt ggataagcat tttcgctaat catccatacg cattctgcca
     2761 agtacgcttc atcaaaatct ttttttctgc aaatcaacta atctcactca ttttctaaaa
     2821 gttttatcat atccaacgca tcttcttttg tttcagtgta atatcttttt ttgatcgttg
     2881 ttgcttgaaa ccccaatttc cgataaagtc tttgtgcatc ttgattactc atacgcactt
     2941 ctaatgacat cgtctcacag cgattcatta tagcaaagtt ctcgatttcg tcaataagca
     3001 gcgaaccgat tcgtttacgc tgatatgccg gtcgaacggc aatattcgtg atatggcaat
     3061 ctaaaccgaa cgctcttgaa ccgataaaac caatcattct tccattatca aagataccta
     3121 gatatagatg aggaagcggt gacttcaatt ctgatataaa ggcacttttt gtccatggca
     3181 attcgccatt atagacttct cgttcgacat ccagcagctc tttgatatct tctgtagtca
     3241 attctcttag tacgtaacga cgatcgattt gttcgactgt tttttctgta taaatcattt
     3301 tatcttgaaa aaagcttctg gcatacgctc taaatttttt caacataact ttcgtctcca
     3361 ggtgtgtgcg tctccaacca tttttcttct gcttctactc gttttaaata gcgaggaaga
     3421 aaagcttgtt tctctgcaac tggttcaact aaagcaccta attgagccac agttacacca
     3481 cttggaatat cccatgctgg aactgtatta atcatcgcac taggcaatat ttcctttatt
     3541 tcagagaaga atttgtgaca atcactcccg acgaaataaa tagcttcatt cagcttttgt
     3601 agttgtgtga acaaatcaga agcagaaata tgtacatctt caagtacagt ttctaatcta
     3661 ttttctttcc agcggtatgc cccagcgtat acattttttc ttcgagcatc aaacatgggc
     3721 acaataatat tatccacacc gatgcaatta gccgcgatgg ttttcagact agatactcct
     3781 actagctctt tatctaatgt ataagcaatc gtttttgctg tcgttacacc tatacgaagc
     3841 cctgtataag aaccgggacc atcagaaacc gcgattcggt caatgtcttt tggacttatt
     3901 tgaagatctg caaacaattg gtcgattgca ggcatcagtg ttacactgtg gttgcgtttg
     3961 atatttgttt gtatttgtcc aagaatttgt tcgtcttcta ccacaccgat cgccaaagtt
     4021 tggtttgcag tatctattcc taatgtaatc atatatttga tcctttctgc tatgacattc
     4081 tcgccttcca ttgtagcata cttttttatt ctgaggatga aaataccaaa ggcaaaacta
     4141 gtacaataat acatgattat ctgctggcgg gcaaattttg ccccttcctg aaacacaaaa
     4201 aagctgcggt atcactccat tttcccacag cttttccatc ttttatttcg ttgattctcg
     4261 aataatcaac tctgtttcca caataatctt tcttgaaacc ggtgctttgg aattacttaa
     4321 ttgaagaatc gtctccactg ctagctctcc catccattcg gtataaactt ttacagtaga
     4381 aagcgagggc gttacatatt tcgctacact gacatcattg aagcccatca cagaaatatc
     4441 ttctggtacc actaaacctt tttcttgaat cgctttcagt gcgccaatcg ctaatgcatc
     4501 attagctgca aaaagcgcat caggaacatg cggattcgtt tttaaatatt gcttcactgc
     4561 ttgatatcct gactcgacag aaaaatctgc ttcgataatt ggaccttcgt cgtagccttt
     4621 ttctgccaaa acttgttgaa atgccataat tctgggatct tttagccgct gatggttttt
     4681 cttcgtatat tctgttccag caagcatagc gatatgttgc gctttatggg agacaaagtg
     4741 ttcaatgact ttttgaacac ttgacttaaa gtcgacaact agagagtcga ttccttgaga
     4801 agtgccgtca gaatctacca aaagcaaggc attttgggat cctttcattt ctaataactg
     4861 agcttcgtcg aattttccta gtgcgatcgt tccatctgcc ggaagatcac taatggaatc
     4921 ccacgtttct ttcaataaag taacgtttgc ttcttctgcc tttttttcga ttcctaggcg
     4981 aatagataaa taataaagat cttcaagctc ttccgtatcg tcgtaccact ggatcaggcg
     5041 aagcgtttgc ttagtatttg ctgcattttt acgatgtttc gtataattta agcgttctgc
     5101 tgcttcaaaa attttttgct ttgtggctaa gccaacggaa agatcaggat catagttgag
     5161 aactcttgaa acagtagcag gagaaactcc tgccaactgc gcaatgtctt taattgttgc
     5221 catttgtctc tcctcctttt ttacgtttat aaaatcgcag taaatcgttc aaacgctgcc
     5281 tgtccttttt catctcgctt gaatactcca gcgtcttcca gtacacgtgc aaaaacctga
     5341 cctacagatt tttgtacgac atcggtgacc gtatctttcg tcaaatcagg atgttctttt
     5401 tttaattgtt ctgcccacgg acgatgatat tcagccatct ggttttcttc tcctaataag
     5461 aaatgctcta cttctactag ttctggtttc aaacgaggtg gtaaaacagc aagccccatt
     5521 acttcgatca acccaatgtt ctcctttttg atatgttgga catctttatg tggatgaaaa
     5581 atcccatcag gatgttcttc tgaaacattg ttgtctcgaa ggacaaggtc caactcaaag
     5641 taattgtccc ttcttcgtgc aattggcgta atcgtatggt gtggtgtacc atctgctgaa
     5701 aaagctctga tagatactgt ttcatccgaa tatgctttcc atttttccaa tactgagtta
     5761 gctgcttcaa taagcaagtt tttctctgtt gattgcaaac ggatcacaga catcggccat
     5821 ttcacgactc ctgcgatgac ttgcggataa tcttctagtt tgataagttt ttctatcggt
     5881 gcttttgcca taggaaattc atgacgtcct gcctgataat gatcatgaga aagaatcgat
     5941 cctccaacaa ttggtaagtc agcattcgaa ccaacaaagt aatgaggcaa gacttcagta
     6001 atccgaagca gtctagcaaa tgtttccttt gtgattttca tcgggcggtg ttgttctgat
     6061 aaaatgatcg aatgttcatt gtaataagca taaggggaat attggaatcc ccagctttca
     6121 ccgtctagat tcattcgaat aatccggtga ttggtgcggg ctggatagtt caagcgacct
     6181 ctatatcctt cgttttccat gcaaagcatg caggtaggat agtctacttt taccgcctcg
     6241 cgttctgctg cgatttgttt tggatctttt tctggcttag ataaattgat tgtgatttcc
     6301 agttcaccat actccgactc tgccggaaat ttgatgtttt ttgcgatggc tcttgttttg
     6361 atgtagtcat ttttcttact taaattaaag aaatactcag ttgcttcctc tgggcttttc
     6421 gaatagtgct gtgcaaagaa tgcattgacg actgaaggtg gcggtgtgag aaaatccatt
     6481 aattgcgctt caaaaatctc ttgttctgct ggcaattctg aaagaacacc attttctctt
     6541 gccttagcga caagctgatc caataagtct acagattctt ccttaactgg tcttacttca
     6601 atgctttcta gagcatcttc tcctaccatc cctaaaagac gattctgtaa atataagcgg
     6661 tccatttcca tccatccgcc agcttcgatc gcaagtgtgg caaaatcaca aatcgtttga
     6721 ctgattgaca tatctcattc ccctattctt cataaccatt tggatggctg acatgccaat
     6781 cccatgctgt tttgataatc gctttgatat ctgtgtattc aggtttccag cccagaatct
     6841 ctctcgcttt ttcacttgaa gcgactaatc ggctaggatc tccagctctt cttggtgcta
     6901 tttctgctgg aatttctttt cctgtcactt ctctagctgc ttctagcatt tccttgactg
     6961 agtatccttt gctgctgcct aaattgaaga agttgctttc atttccttct ttcaagtatt
     7021 ccaaagctaa aatatgtgca gcaatcaaat cttctacttg aacataatct ctgatacatg
     7081 ttccatctgg tgtatcataa tcgtctccat aaacagccaa ggcttttctt tgaccaagag
     7141 ctacttgtaa aatgattggg acaagatgcg tttctggtgt atgatcttct ccgatagatg
     7201 catctgcttt tgctccggct acattgaagt agcgcaaagc gacatagcgc atgccatatg
     7261 cttggtcaca ccacttcatc attttttcca tcatcaattt actttcacca tacggatttt
     7321 taggattagc tggtgtatct tctgtgattg gagactcttt cggctctcca taagttgctg
     7381 cagtagaaga gaatacgatt ttattcacgt tgaactcgtg catgacttca agtaagattt
     7441 gcataccgta tacgttgttg ttgaaataca tcaatggttt ttcgacagat tctcctacta
     7501 aagagcttgc agcaaaatgg attactcctt caatcggttc cttttcaaat acggaacgta
     7561 gaaattcttt atcccggatg tctccttcat aaaaatgagc atctggatgt actgcttgtt
     7621 tgtgacctgt gagcaaatta tctaccacag ctacttgata ccccttttga actagctggt
     7681 ctacagcgtg cgaaccgata taaccggctc cgcctagtac taaaattgtc ataaaaaaca
     7741 ctcctttgat tagtaaaatt gtgtttgttg attctatata atttaaaaaa cg
//
LOCUS       DAYYCY010000116         7707 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000116 DAYYCY010000000
VERSION     DAYYCY010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7707
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.116"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            63..2390
                     /locus_tag="ACHAZB_002074"
     CDS             63..2390
                     /locus_tag="ACHAZB_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBG domain-containing protein"
                     /protein_id="HFM8614711.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            2387..3301
                     /locus_tag="ACHAZB_002075"
                     /pseudo
     CDS             2387..3301
                     /locus_tag="ACHAZB_002075"
                     /inference="COORDINATES: protein motif:HMM:NF027869.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
     gene            3374..3889
                     /locus_tag="ACHAZB_002076"
     CDS             3374..3889
                     /locus_tag="ACHAZB_002076"
                     /inference="COORDINATES: protein motif:HMM:NF037639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prealbumin-like fold domain-containing protein"
                     /protein_id="HFM8614712.1"
                     /translation="MDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFT
                     SSKDGTFSVHGLSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEP
                     KANPTNNGKTPVQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKK
                     QQKGNNRREIK"
     gene            3886..4332
                     /locus_tag="ACHAZB_002077"
     CDS             3886..4332
                     /locus_tag="ACHAZB_002077"
                     /inference="COORDINATES: protein motif:HMM:NF027869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFM8614713.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            4482..5297
                     /locus_tag="ACHAZB_002078"
     CDS             4482..5297
                     /locus_tag="ACHAZB_002078"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFM8614714.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            5503..6675
                     /locus_tag="ACHAZB_002079"
     CDS             5503..6675
                     /locus_tag="ACHAZB_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFM8614715.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEGSSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(6837..7544)
                     /locus_tag="ACHAZB_002080"
     CDS             complement(6837..7544)
                     /locus_tag="ACHAZB_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8614716.1"
                     /translation="MKKLVALFLVAGSLLSGCGATTSTTQSTSSAQASSSSTTISEST
                     ADSSKEQIEKKKEEEKQKAEKAALEKKVTEAESALSQAEANPTKENYATASAAIQALP
                     ENKQELTSRLAAIDSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAI
                     AQQQAEQARQQAAAEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATL
                     SEAQARGLTPCSKCYGN"
BASE COUNT         2597 a         1452 c         1534 g         2124 t
ORIGIN      
        1 taaaaattaa atttagaata acgtgtaaag gattatttga tgatttttta ttgagaggag
       61 ggatgaaaat gattaagaaa actcaaaata aaatccaacg tatatgtcaa aaacattttg
      121 tttctagact caaagctgta tctaaaagaa aatacttaca tcccattatg atttcgatca
      181 tcttgatttt tactttctta gggagcttat caagtattta tgtaaacgtt tatacagaaa
      241 cgtcaaattt tgctaaccaa acgacagagg agacaaatag tccaccctat tctgaaatac
      301 agtccataga aagcaccaat cagtctagca acaacgagac aacctcaacc acatcctcga
      361 ctcaactgat tgatccgaca gaaaaagaaa tcacagcagc aaaagcagca ggggagaaag
      421 cttattcaga aacaggtaga agccagacat ttagtactgt tgcggccact gcgaatttca
      481 acggacatac cataccagat ggatatacag tgatatatgc caaagatgga gtcattgttg
      541 cggttaacgg atcgatgagt gcggataaaa ctgcgttaca aggcgtaacc aaagcaatcg
      601 cttttattga taaccgtcca gatatccata atctgtttat tggctccaca gtaaatggta
      661 ctactatcac attcccaaaa agtatctata tgggggtggc taccgctgga aatacgatcg
      721 attcagttac acccaatgcg gataaaagtt attcttttac accctcaaca tggggagggc
      781 ttcctgccgg aggaggtatg agtacttttt caatcggtat acagttagca aatgatattc
      841 cattcacggg gtactcgatc aaaaatgggc agatcgttga tggtaaggga aatgtttcaa
      901 attttccaac ctatcaagca gcaacggttc aggtcaatgc gaatcggaca ggaacttttt
      961 cttatacagt caatggtgga gcagcaaaaa tcggaacgta cgggactaca ttatctggaa
     1021 tccatgtggg tgacaaagtg gttatcactc caacagcagt aacgggctat tcttttgcac
     1081 aaacaaacgg ttctttcaca gccctagaaa gtaacgtcga tacggtgacc tatacaggca
     1141 aacaaacacc aatcactatt aagtatcaag actattttgg acagatgatt gctccttcta
     1201 aaataatgaa tcttacttac ggaagtgctg cacaagacct gacgactaat acgcctacta
     1261 tcaacggata tacctttaca gctgtttcag cgatagaaac aaagaaccaa tcagcaatga
     1321 gtgttttagc tagtttagat acaaatggaa atgtaatcgt aaaggacgca aatggaaaac
     1381 agatttctga agtcatccta tattataaga caaatgcaac agtatcaatc aatgcaaatg
     1441 gcagtaagta ctatgatggt ctttctgttg taccgatcgt taaatatgcc tttaatgata
     1501 aaacagaatc aacatccctt ttaaacaatg atctcagtat tgatcaaatc acatggaatc
     1561 caactgattt caaagcgatg aatgaaaagg gagttgaagt aactgcgcct actgaaattg
     1621 gaacctatac gtcttggcaa ttgacaaaaa gcggactagc taaactagct gccagaacaa
     1681 actatttatt tactatcgtg cagacatctg atgtctatac gattaagcaa atcagcggtg
     1741 cggtagagct cagtggcagt aagacatatg atggaaaagc aggtgtcccg tcaatccatg
     1801 tcaagctagc agagggtgtc actagttcgc ttactccagt acaagtggca cttagcagta
     1861 tggattacac agcagatgca caatcagcaa aaaatatggt taatgctggc agctatacaa
     1921 ttaaactaac gaatagcgga atagacaaag tcaaagcagc aaatcccaag ttgtcattta
     1981 ctgatatagc caacacatca ggaacttata ccatctataa agctaacgca gtgattactg
     2041 tcgatgacgc tgcctttaat tatgatgccc aaagtcactc gatttcggtt ggaaatctcc
     2101 acgttaccgg tgttgtctta ggcgaatcat tggattatac cctaacagtt aacagccgga
     2161 cagatattgg atcacaaata gtaacgatta gtttaggcga aggaagtgta aacaataatt
     2221 atacgattat cgcaacagat accgctaagt tgacgatcaa tccgactctc ttgcttcctt
     2281 cgacaggtgg gataggtcta ctgccctttg tctttttagg actgatcttt attttttctg
     2341 gcttctttta ttttatccat cgtaagaaaa aagcaggtga acagcgatga aaaaaacaaa
     2401 aaatgtacgt gtggatatat ttgctgttct cttttttatc ctactggtct tttttatagg
     2461 ttctccgact gtgtcagcta gagaatcacc tattacattt actttgcata atcgaatgtt
     2521 cgaaagcgat aagatgccaa gtatcagtca aaacgacgga ataaatatcc cggataactt
     2581 tcaaggagta aaagggttga acggcgttac tttcgctata tatgatgtct cggatgaatt
     2641 ttacaagctg cggtcagaag gaagtagtgt ggaagacgca caaagaaaac tagcacaaaa
     2701 aagtgatagt gaaaaaatac tggcagaaaa tgtgacaaaa acagtagacg aggaagaggg
     2761 cattgcttct tttagtgcat cggacaaaga tgagcaaggt cgtgacgctg tttaccgatt
     2821 tacggaaata aagacttcag attaagtaaa agaaaaatca gcaccatttg tcgttgtgct
     2881 tcctgtgaca gattcatcta atcataaatt gacgaccatc cacttgtatc caaagagcga
     2941 acaaaaaata cctgctgcac tcacattaac aaaaacagta gagaataagc agacagattt
     3001 tgcagatggg gataaagtcc cttatctcat caccgtaaca attcctgaga acatcaacga
     3061 gataggaacc tatacgatca aagatacagc tgatcctcag ttatggctag aaccagaaac
     3121 gatcgatttt ttgattggtg gagacaagat tcatacattt cacacacaaa aaataaaaca
     3181 tggttttgtc ttatcagatt ctggaaaaga tcttagttcg tttgctggaa aaaaattaac
     3241 gatccgttac caaatgacat tgaaagaaaa cagtgaaaaa acgacatttg ataacgatgt
     3301 acgtttgacg actgacaatc aaacagtaga aacacatctt gccatccaaa caggaggcaa
     3361 acaatttgtc aaagtggatc ggcaaacgaa acaaaaacta gcagatgctc aatttctagt
     3421 caaaaaaaat gaccaatact tagcgcaaga aaacgggata aatcattggg tagccaaaga
     3481 tgattcaaga gcaacgattt ttacttccag taaagatgga acattttctg tccatggatt
     3541 gtcttttgga agttacgaac tagtggagat taaaccgcct aaaggatata tattgagtca
     3601 atcaaccatt ctctttgaag ttgaaaaagg cagttatgat ccgcttcatc ctataccatt
     3661 ggaaatcatc aacgaaccaa aagcaaatcc gacgaataat ggaaaaacgc cagttcagat
     3721 gcagacaaat ggtccatcaa atgaaaaaaa tggaggaagc tttccaaaaa caagcgaaga
     3781 gatgcttgga ggtttctcaa ttttagggtt attgctagtc ctttcaacag ggacagcttg
     3841 gttttataaa aaacaacaaa aaggaaacaa taggagggaa ataaaatgaa aaacatcaaa
     3901 acaaaaatat ggacgttttt aggaactgct atcatgctgc ttcctttcgt tctcggccta
     3961 ggaactgcag aggtttctgc agccgtatca cctacaccag aaaatgttac ggttaatttg
     4021 cataaattga aattcacttc agcaccagaa aatcaaataa acaacggaac ggaattgaca
     4081 tttccgaata gcgaaccgtt gaacggcgtt gaattcaacg tatatgacat cactgctacg
     4141 tactatccaa gtaaagacac agcagttcca gcagacgcta cacccttcgc aagtgtaacg
     4201 acttctggtg aaggattagc taacctgact cttccaggta aatctgatgg gaaagatgct
     4261 gtatacgtct ttgtagaaac accaaagccc ggtgtcgaaa cgtcgccaaa tatcgtgtta
     4321 agcttaccct agaccctgac aaacctttag acacgattca cctatatgct aaaaatgtca
     4381 tcaaagaaag caatatcaaa atccagaaag ctgggaactt ttcaggggta gataagttag
     4441 caggggcgca attccagatc aaaaattcag agaatcaatt cgtgaccggt tttgatgaaa
     4501 acactggtgt cgctctctat gatcctgctg agtcagcgtt gacattcaca actgatactg
     4561 aaggatccat cagtgtcaat ggattaatag acggcaatta tactttagtt gaaacaaaag
     4621 caccagatgg ttatcaatta accaatccac aaaaagaaac tacttttata gtaaaaaacg
     4681 gaaaattgca acaaacaacc ggtatcatcc atggcgcaga tgaagccaat cttgggaatc
     4741 caaacggtgt agtcaatcgg gaaaatacaa ctacggataa cacgattact gtagaaaatg
     4801 tccaaaacta cggtgatttc cacttcatca aacaagatgt gaacacgcaa gaaaaattag
     4861 ccgatgcaga attttctatt ctaagagcaa aagatccaac tgttaaatta atgaaaaaaa
     4921 tagcaaacct tgcctcagga gaatacccat atgcttggtc agatgaagtc acaggttctg
     4981 actgggaaga agtgaaattg atttctgatt cagaagggaa attttcaatc gaagggctga
     5041 aacaaggggc atactgcctt caagaaacaa aagcaccaga aggctacgca ttacctaaga
     5101 atgaagctgc gtacactgaa tttacagtag atgatgatcc aacaacttct gatgaaacaa
     5161 cgatcgataa ccaacctaaa ggaatcttgc cttcaactgg tggtatgggg atcatcgtat
     5221 tcatcttggt aggtactgct ttagttggag gagcagtgat ctatttcaaa aaacgccatc
     5281 aagaaacaga agcttaaact attggaaaca aaaatcatac gataaataaa cgatagtgat
     5341 tcaaatgggg agaaaggttg tgacaagtgg tcggtctctt ggtattcgct caacaaacgg
     5401 caaaatagga ggaactattt tgccgtttgc ttcgctaata ctcaggtcaa ggcgctgatt
     5461 tcacaacctc ttcatataca tattagaaaa taggtggaca tgatgaataa aagcagaata
     5521 agaatactgg acatcttcat ggcaatcatt ttagtcgttg ggatcgggat cttttcttat
     5581 ccttttgtag aagattcact gaatgatttt cttgcccagc aaatgatcat ccattatcaa
     5641 aagcaagcat cgaataagaa ttcagaggaa ataaaaaaac agcaggaaaa aatgacaaag
     5701 aaaaaccaac agttggcaga gcaaaacgta tcaccaggga taggcagttt caaccaagca
     5761 gttgatgcaa aagcattaaa agatttacct agtaatgcct tttttatgga acatatgctt
     5821 ggagtcatcg aaatcccaaa aatcaatgtc agcttaccta tattcgatca gacaacggaa
     5881 atatttttgc aaaaaggaac gtcattattg gaaggaagtt cttatccgac tggcgggaaa
     5941 aacacccatg ccgttctttc tgggcatcgt ggactcccag aagcaaaatt atttacagat
     6001 ttgcctaagt tgaaaaaagg agaccagttt ttcatccaga tcaatggaaa gacattggcg
     6061 tatcaagtag aaaaaatcca agtggtctta cctgacgaag tagattcttt gggtatccaa
     6121 aaaggcagag atctcgtgac tttgcttacc tgcactcctt atatggtcaa tacacatcga
     6181 ctgcttgtga cggggcatcg tattccttat caagcaaaag aggcaaaaaa agcgatccaa
     6241 ggaattgatc aatggaaaaa atggaaattt tttatttggt ttatcggtat actacttgga
     6301 tcaatcggct tagtttggct actcatcgct tatttagatt ctttggccat cgctaagcga
     6361 aactatcctt tatcttttta tgtaaagaac acaaacggac gaccaatcga aggaatggtc
     6421 ttttctgtaa aaacgctcaa cggcaaacac tatattacta gagaaaaagt tccatttgtg
     6481 aaagcttctg atgaatacgg actagtgaga ttttctgatt taaaaggcag gaactatcgc
     6541 ttgcaacatg aggagctcct gcttaaaatc catgtaaaac ataaacattc gaaacagttt
     6601 tcaatgaaat tgaaaaaagg aagatataaa ctgcgaaaag aaaaagaggt gtactatttg
     6661 attgagaaag aatgaatgaa gatgtccaaa aaagcagagg gaacagacta aatgaaaaaa
     6721 gagagaagct gacgaaatgg acgtgtaaac tgctattttt ggtttactat ctggaacgcc
     6781 agcttctatt tttttatgtt tcagaagaat tttattatta tagtataact tttgaattag
     6841 tttccataac atttggagca aggggttaaa ccacgagctt gagcttcgct taaagtggca
     6901 ggggtatacg taccatttcc acatttatgt gtatggtatt ttgaaccggt cggtgtgaca
     6961 tagacagttt gttcattatt ttgctcagga gctgcggctt gtgcctgctg agaagcctgt
     7021 tcagctgctg cttgctgtcg cgcctgttct gcttgttgct gtgcgattgc ttcttgcctc
     7081 gcctgttctg cttgttgctg tgcgattgct tcttgttgcg cttgttctgc ttgttgtcgt
     7141 gcaatttctt cttgcttttt ccgttcttct tctgcttctt ttgctttgat tgtggaatca
     7201 attgcagcaa gacggcttgt caactcttgt ttgttttcag gtagcgcttg gatagctgct
     7261 gaagcagttg catagttttc ttttgtagga tttgcttcag cttgtgacaa agcagattcc
     7321 gcttctgtca cttttttttc tagtgcagct ttttccgctt tttgtttctc ttcttctttt
     7381 ttcttttcaa tttgttcttt cgatgaatct gccgtacttt cggagatcgt ggtactgctg
     7441 ctggaagcct gagctgaact tgtgctttga gtagtagacg tagtagcacc acacccactc
     7501 aacaaacttc ctgcgaccaa aaacaacgca actaattttt tcactaaatt tcctccttaa
     7561 gtaaaaaaca accgttttca ataaatgtcc ttttcaattg tttacttact tgttataata
     7621 attgtgttat gaatagtgat tgaggaaact taatctaatc ccgtgttgat acacgggatt
     7681 ttttgctata gttctgttat taagtat
//
LOCUS       DAYYCY010000117         7681 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000117 DAYYCY010000000
VERSION     DAYYCY010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7681
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.117"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..209)
                     /locus_tag="ACHAZB_002081"
     CDS             complement(<1..209)
                     /locus_tag="ACHAZB_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HFM8614717.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYL"
     gene            complement(206..724)
                     /gene="msrA"
                     /locus_tag="ACHAZB_002082"
     CDS             complement(206..724)
                     /gene="msrA"
                     /locus_tag="ACHAZB_002082"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFM8614718.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(744..1457)
                     /locus_tag="ACHAZB_002083"
     CDS             complement(744..1457)
                     /locus_tag="ACHAZB_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HFM8614719.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(1460..2356)
                     /locus_tag="ACHAZB_002084"
     CDS             complement(1460..2356)
                     /locus_tag="ACHAZB_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFM8614720.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(2514..3356)
                     /locus_tag="ACHAZB_002085"
     CDS             complement(2514..3356)
                     /locus_tag="ACHAZB_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFM8614721.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            3488..4141
                     /locus_tag="ACHAZB_002086"
     CDS             3488..4141
                     /locus_tag="ACHAZB_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFM8614722.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(4204..4731)
                     /locus_tag="ACHAZB_002087"
     CDS             complement(4204..4731)
                     /locus_tag="ACHAZB_002087"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFM8614723.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(4751..5698)
                     /locus_tag="ACHAZB_002088"
     CDS             complement(4751..5698)
                     /locus_tag="ACHAZB_002088"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFM8614724.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(5710..7593)
                     /locus_tag="ACHAZB_002089"
     CDS             complement(5710..7593)
                     /locus_tag="ACHAZB_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFM8614725.1"
                     /translation="MKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKTS
                     LLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVRA
                     YEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIST
                     LSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDRY
                     FLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMRA
                     GVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRINDQ
                     TLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQE
                     NEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEKR
                     RLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVAD
                     KLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKEW
                     ETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSEW
                     VED"
BASE COUNT         1987 a         1575 c         1350 g         2769 t
ORIGIN      
        1 tctaaatatt tttcccatgc ttcgtcaaag atatccatgg acggcaaata gtcgacattc
       61 agttccagat aattagaaat ttcttcgtag ctagagctgt gcttaggaaa ttggatgtct
      121 ttaccggctt ctgtagcgaa aacagataaa gcatcatgtg tgccaccgcc tcgcagtgtc
      181 attaaataat gaaaaaagct tcgattcacc cttcgttcct ccaattttct tcgataaagg
      241 cagcccggcg tgcatggttt cttttaaaat tttcaggatg ctttttataa tactcttgat
      301 ggtcttcttc tgctagataa aatggttggg ctggttcgat ttttgtaacg attggctgtt
      361 cgaaacgtcc actttcagct aactgtttct tacttttttc agcaatgatc cgctgttctt
      421 ctgtcaaata aaagatcacc gggcgataat tgtcaccgcg atcctcgaat tgtccgaaag
      481 cgtcagttgg atcagtttgc tgccagtaaa gttctactaa ttgttcatat gaaatcaatg
      541 ttggatcata gataatctcc actgcttctg tatgtccagt cgtttggctt tttacttgtt
      601 cataagtcgg atctggtaca tgccctccag tatatcctga catcactgta tggataccag
      661 gaagcgtgtc gaaaggctgg atcatacacc agaagcagcc tcctgcaaat attgctcgtt
      721 ccattatgat tccccctcta atactactca tccgatgatt ttttattttc ttttgtcttg
      781 ctttcagtac tgctggtgct agttttgttg gtatttgagt tgtttgaagc atcagaggta
      841 tcttttggca aatatagact aaaccgaatg tcatcattta ctaagtcgat tttatctgct
      901 ttaatataca tcccgttttg cattcggaat tgatccaacc taagcatgat agtcagatca
      961 tctgtattga cttcgatcca atctggtaat ttatagtttc tcttgatcat attcatcaca
     1021 tctttgattg gcaaactcaa tgttccgata gaaagacttc tagcttttag ttggacattt
     1081 ccgttttcca tcacatacgg atcaaaatat aaatagaaat cgataggaaa acctagtact
     1141 tcgaaagtac cattgagtag tgcttcattt tccaaataaa atttatattc aatatcacta
     1201 tctttttgat actcactaag gaaaaagctg atgatttgat tgactttttc tttatttgaa
     1261 ttgatcgtta agacaggatc tccttgtctt tccgtaagag ctggtatttc tgggagttct
     1321 ggttcccgat tagcaaaaat acggctgcct aaaaatacgg tcgaaccaag tactacccca
     1381 agaaggataa ggaaggcgaa tttccagtaa tttgttttct ttgttttgtt tgaagatacg
     1441 tcagatcttg ttttcatgtt tattgatcat cctccttctc taaccattgg ttttgtgtct
     1501 cgatcagctg atctctcact gcatttgcca tcaattgata acctaaatta tttggatgga
     1561 aatgatcttc ttcatagagc acattatttt tgatgccatt tagattttct gtcgtgctag
     1621 ttgtttgatc tgttccttct tctgtgacac ctacttcctg attcaatcct tgataaagca
     1681 aatcattgat tgggataaag tagcagttcg gcattttttt tgtcaccgtt tctgttcctt
     1741 tgttccaatt gttaacaatt gtctgcatgt cagtgatctc aggaaagttc aaataaaatg
     1801 gattgtagat gcctaaaatg taaataggtg cggttttatt gagggatcga aattcttcca
     1861 atagttctgt gacattttcc tgatagtttt tcagtggctt tttaaaggaa ttgaccgtta
     1921 acccgaataa attattttgg ataactttca tcaaatcatt accgccaaca gtcagtgtga
     1981 taaaatcagc tgttttgata ttgtttcgaa tttcttcttt tgtttttact cgctttaaga
     2041 tctggtcact tcgttcacca tttacaccat agttttctgt ttcaaccgcg gtcaaattat
     2101 aacggtcttt tagatcattt gcgacaaggg gaacgaatcc tccttgcttg gtttgatctc
     2161 ctactccttc agtcagcgaa tctccgatag ctatgtagtg aagtttttct tttttatttg
     2221 gatcttctct cacagtagtt actggtttca ttagtgggcc agctttcggt atagaagcag
     2281 ctaaacctgc atagatgatg atcgcaatta ataatgggaa caaaacgagg atcaaatgtc
     2341 gtttcgttaa tttcattttt tcacctcttg tattcgattg gttctaatgt tatcaaaaat
     2401 tttatcaaaa aggtagtctt tatgaaaaaa gaaaacaatt attttcctaa ttttataaag
     2461 aaaaagagaa taagctgcaa gcctttcagg ctgagggctt atcctctttt cgactaatct
     2521 acataataca tgattgcaaa cgcgtcttta ccagtatgcg tagcgataac cggattcgta
     2581 tgcagtacag ggatatgcat gtccgggaat aattcttgca aaccctcttt gaattcttgc
     2641 gccaattctt ttgcatccgc atgtgaaata ccgatttgtc gcacatttgg caattctttc
     2701 agttcatttt tcaaattatt aaaccattta tcgaacgttt tctttcctcg ccccttagtt
     2761 acagggatta attcagaatg ggcgaaattc atcaccacgc gtatgttcaa aacactagac
     2821 agcaaaccag ttgttcggct gattcttcca ccttttacca agttatctag tgtggatatt
     2881 ccgatgtata attttgtatt gtcttgtact tttttgactt cttcaaggat tttctctact
     2941 gttttacctt cttttgctaa ttcagctgct cgaattacct gaaaagataa tccttggtca
     3001 gtggtatcac tgtcgataac cgttactttc gtttttgtta gattactggc ttgtcttgct
     3061 gcttctactg ttccactcaa gccttttgtc atatggatcg aaatcacttc actgccgtct
     3121 tctcctagct catcatacat cgagatgaat tctccaattg gtggttggct tgttttgggc
     3181 agattttttg ctttcgccat catttccata aatctttcgc cgtctagttg gtcatcatcg
     3241 ggatatactg ttccgtcgat catgatggat agcggcatga cacgaatgcc taaagcatcg
     3301 cgtacatttt tttccatcgt acatgaagaa tctgttacga tttttacatt tgtcattatt
     3361 ttctctctct ctttctttaa caagatgcct tattcatgtt aaactacatg taagacaatt
     3421 tctattttta gtataacaga caaataatga tttttcatta attacaccta gtaaaggagc
     3481 cgttttcatg gaaaaaacga aatttaatcg aacgtatgaa atattaaatg aagtgtttaa
     3541 tgcagtcacc catggaattg ggactggatt gagtatcgcg ggactagtgg ttcttttagt
     3601 aaaaggtgcc cacttgcatt cctccctcca tattgtctct tatgctatct atggatcgat
     3661 gatgattctt ttatttttaa cgtctacttt gtttcattca ttgattttta caaaagcaaa
     3721 aaaggtgttc caagtattcg atcatagttc gatttttctt ttgattgcag gaagttatac
     3781 tcctttttgt ttgctgagta tccgaggatg gctaggttgg actttatttg tattaatctg
     3841 gttgtttgcg attcttggca ttatatataa agcgttgacg ttgcataaac gggagagagt
     3901 atcaaaagta tctacgatca tctatatttt tatggggtgg ctctgtgtcg tggctgctgt
     3961 tcctctttat cactctcttg gaatgactgg tgttattttg atggtagcag gcgggctttc
     4021 ttacacgata ggcgcattct tttatagctt gaattctatc cgctatatgc atgttgtttg
     4081 gcatttgttt gttatgttag gtgcaggttt tatgtacttc tctgttttat tctttactta
     4141 ataaaaaaaa taaagctatg gatgggacag ctctaaataa cagctagtct atccatagct
     4201 tttttatatc gtcaattcgc ctgtcccgct ttttacagga ttctcattac gctgatacgt
     4261 ttcaaattga tgcggataac gatttttttc atccatttct ccttgactag tattgatcag
     4321 tgaccaatca gaccagtcaa caggtggaaa ataagcatct ccatcgaata catgatggat
     4381 gactgtacgg tataatatcg tacaaaatgg cagaaattct tgataaacag ctgaccctcc
     4441 agcgatgaaa aagataccgt ctgtttcttt ttccagagca agcacgtctt ccacactatg
     4501 catgacatgc acgccttctg ctttatagga agaatctctc gtcaaaacaa tcgtctgacg
     4561 attcggcaac ggacggctac ccatcccttc gaatgttttt cgtcccatca ctagtgtatt
     4621 tgcttctgtc atttgtttga agaatttcag atcatttggc aaacgccaag gaagacggtc
     4681 ttcttttcca atcaatccgt tttcatcctg tgcccaaatt gcagctaaca ttttctcatc
     4741 ctttctgatt ctaaacagcg ataggagctt tgatcgccgg atgtgggtca tatccttcta
     4801 attggatatc ttccatctca aaatcaaata ctgactgctt ttcctgattc aaaactaaag
     4861 tcggaaatgc gcgtatttct cggctcaatt gttctttcat ttgttcgata tgattgatat
     4921 aaagatgagc atctcctaat gtgtgaacaa attcgccaac cgctaaacct gtttcatgtg
     4981 caattaggtg agtcagcaac gcataacttg caatgttgaa cggaacgcct agaaacacat
     5041 ccccactgcg ttgatacaat tggcagctta gccgtccctc ttgaacataa aattgaaaca
     5101 tcgtatgaca aggaggcaaa gccatgctag gaacatcttc cggattccat gcagaaacga
     5161 tcaatcttct agaatcagga gtctttttga ttgcttcgat cacttgtttg atttgatcga
     5221 taaagccacc gtcttttgtt tcccagtgac gccattgata gccgtagatg tgtccaagat
     5281 cgccgtatgt ttcagcaaag gaatcatcgg ttaggatctt ctcgcaaaaa agcttgctct
     5341 gctttttata ttcttcattg aattcaggat ctgtcaaaca tctatggcca aaatcagtca
     5401 tatctggtcc attatagtct ggacttttga tgtagcgttc aaatgcccat tcatcccaaa
     5461 tatggttttt attttgtaat agatagcgga tattcgtatc tccttttaaa aaccaaagca
     5521 attcgctttt gatcaatcca aaaggtactc gtttcgttgt taacaaagga aacccttttt
     5581 gcagatcaaa ccgcatttgg tatccgaata agcttaatgt gcctgtgcct gttctgtctt
     5641 ctttacgatg cccttcttcc aacaattttc ttcctaagtc aagatacgct tgttccatcc
     5701 tttttcctcc taatcttcta cccattcact caagtattcc caccgtgcca ttttttcttc
     5761 aagttgtgct tctgtttcag atacgtcgtt ttgcaattct tgtaatcgag tgaaattatc
     5821 tccttgatga ttcatctctt cttggagcaa tgagattttt tcttcaagtt ctgcaatctc
     5881 atcttcaatc gtttcccatt ctttttgttc catgtaagat aatttcttct tttcagcagg
     5941 ttccttgctc tgtatttttg gtttcgcttt ttcagcaggc tgctgggtct ctttttgctt
     6001 cagtaaataa ctggacatgt ttccgtaata aagttcttgt tttccgtttc cttggaatac
     6061 taataactta tctgctacct tgtccaagaa ataacggtca tgggaaacag tgatcacagc
     6121 acctggaaaa cttctaaaat aatcttccaa aatcgtcaat gttgctatgt ccaaatcatt
     6181 ggtcggttca tccaataata aaacattagg ttgctgaatc agcagtttca acaaaaataa
     6241 ccggcgtttt tctccgcctg aaagtttgcc tatcagtgtc ccatgcataa atcttggaaa
     6301 taaaaatcgt tcaagtaatt ctgctactcc gatttgactg ccatctgctg tttttgcttc
     6361 ttcagcagct tcttgtaagt aagcaatcat ccgtttatct ggtgccattt cttcattttc
     6421 ttgtgtataa tacgcaagat gaaccgtttc accgatagac attgtaccgc tatccagggg
     6481 aattctgcca gctagaatat taagcaatgt cgattttccc gcaccatttt taccagtaat
     6541 acctagtctt tcccgagatt gaatcaataa atctaaatgt tctagcagtg tttggtcatt
     6601 tatacggtaa ctgccatctt tgatttccag tacttttttc cctaaacgtt gtgtcgctag
     6661 gttcagctct aaatcatctt cttggtttac ttggtacaag ttctccttta gatcttcgaa
     6721 acggttgatc cgtgcttgct gcttggtgcc acgtgcctgt acgccagccc tcatccaagc
     6781 taattcctgt ttataaagcc gcttgcgctt ttcttcttgt tctacttcta cacgatcacg
     6841 ttctgctttt tctaagacgt aagcttcata atttccttta tactcgtata attttccaaa
     6901 tgaaagttcg aagattcggt ttgctactct atctagaaaa taacgatcgt gggtaaccat
     6961 caatagtgcg ccacggtatt gtttcaaata gttttccaac cactcgatcg cttcataatc
     7021 taaatggttt gttggttcgt ctagtaatag taaatcgggt tcatcaatca acacttgcgc
     7081 cagactaact cgttttttct gaccacctga taaagtactg atttttttat ctaattcact
     7141 gattcctaat ttttgcaaaa taatctttgc gttggtatct gtagtccaag catcttcttt
     7201 attcatttgt tcttctgctt tagcataacg tttttgcaca tcagggtctt ctccgttttc
     7261 ccctagttcg atcaatgctt cctcatatgc tcgaacagtt tggatcaagg ggctgtttcc
     7321 ttgaaagact gcttggagaa cagttaattc atcggaaaat tcagctgctt gagaaagata
     7381 accaatacgg taatcagttg gataaaacac tgtttgccga tcaccgtcgc tgctatcgat
     7441 accggcaata atatttaaga gacttgtttt acccgtcccg ttcgtcccaa tcaacccgat
     7501 acggtctttt tcgtggacca aaaaagaaat ctggtcaaac aaatcttttt caccataagt
     7561 ttttttgagg tcagttactt ttaattcttt caacatgttc cctccttatc ttggatattg
     7621 tatcataaaa atagtgaact tcaggaaaat ttttttggta tttgttcttt ttgtgttctc
     7681 a
//
LOCUS       DAYYCY010000118         7628 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000118 DAYYCY010000000
VERSION     DAYYCY010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7628
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.118"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(111..635)
                     /locus_tag="ACHAZB_002090"
     CDS             complement(111..635)
                     /locus_tag="ACHAZB_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HFM8614726.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(628..1101)
                     /gene="tsaE"
                     /locus_tag="ACHAZB_002091"
     CDS             complement(628..1101)
                     /gene="tsaE"
                     /locus_tag="ACHAZB_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFM8614727.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1224..2204)
                     /gene="pta"
                     /locus_tag="ACHAZB_002092"
     CDS             complement(1224..2204)
                     /gene="pta"
                     /locus_tag="ACHAZB_002092"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFM8614728.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2357..3037)
                     /locus_tag="ACHAZB_002093"
     CDS             complement(2357..3037)
                     /locus_tag="ACHAZB_002093"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFM8614729.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3163..4032
                     /locus_tag="ACHAZB_002094"
     CDS             3163..4032
                     /locus_tag="ACHAZB_002094"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8614730.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4058..4573
                     /locus_tag="ACHAZB_002095"
     CDS             4058..4573
                     /locus_tag="ACHAZB_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related/scramblase family protein"
                     /protein_id="HFM8614731.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4620..5183)
                     /locus_tag="ACHAZB_002096"
     CDS             complement(4620..5183)
                     /locus_tag="ACHAZB_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8614732.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5238..5417)
                     /locus_tag="ACHAZB_002097"
     CDS             complement(5238..5417)
                     /locus_tag="ACHAZB_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8614733.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5494..6003)
                     /locus_tag="ACHAZB_002098"
     CDS             complement(5494..6003)
                     /locus_tag="ACHAZB_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8614734.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6099..6479)
                     /locus_tag="ACHAZB_002099"
     CDS             complement(6099..6479)
                     /locus_tag="ACHAZB_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8614735.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            6809..7558
                     /gene="pnuC"
                     /locus_tag="ACHAZB_002100"
     CDS             6809..7558
                     /gene="pnuC"
                     /locus_tag="ACHAZB_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFM8614736.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
BASE COUNT         2169 a         1582 c         1355 g         2522 t
ORIGIN      
        1 accgtttatt caaaaaaaat ggactgcgac acgacttttg tcacagtccc ttcgtgtgtt
       61 tttctgcctt ctcaaaagga tgacagtttc tcactctttt cctatttctt ctattcaatc
      121 aaataactca ttttatatac atctaaaaat tcacctagat ctgttcttgc ccctctaggc
      181 atgaccgctt cgatttggaa gcccattttt cggtaaaggt gtacggctct ttcattacga
      241 acttgaacgt caagctccaa tcgaaaaagt acacccattt ccttcgccca actgatgatt
      301 tcttctagca taagagtgcc tagtcccatt ccccaatatt cctgcagaat actgataccg
      361 acttctccca cgtgagacaa acgaaattga ctgtctgcct tgacagatgc tgttccaata
      421 atcgtccctt catagattgc aagcagcaat aaattattat tgctttcgta tatataatcc
      481 aactcttctt ctaaggtttc tggtggtaac aggagttctg cttcatccaa aactaaaaat
      541 tctgtctcct tattaacttg tcccatcatc aaaagaatgt cttttgcatc tcctggcact
      601 gcttcccgca agatgatccc aacttcttca ctcatgattt tcagcccatt tttctagaat
      661 tcgctgtttg aaggtttcac tttgctcccc gtatgagatt agtttcacat accgcttttc
      721 taaatctgta tcactttttt ctaaaatcag ttccaaataa tcctccggca gtgcttcttc
      781 taatagattt ccccattcaa caacggaaag cccatcacct tcaaaatatt catctaaccc
      841 taaatctgct ccgctagctg ctacccgata aatatccata tgatacaacg gaatccgacc
      901 ttgactatat tctctaataa tcgtgtatgt cggacttttg atcatttgcg ttatccctaa
      961 tccttgggca attcctttcg tcaacgttgt tttacccgca cctagatcac cagtcaatat
     1021 cagattgtca cctggcattg cggaatgacc aattgcctct gcgaattgtt ctgtcattgt
     1081 taatcctgat agttcgatca tttttcatct tctcccgctc ttctttcttt ttgccagtat
     1141 atcgcgtaat gaagtttttt taaagaaaaa atgaactagt caagaacagc agctgctgtt
     1201 tttggctagt tcgcgtgtat ttgttaatca atcaatgttt gtgctgctgt gataatcgat
     1261 aatttatata catcttcttc atttgctcca cgagacaaat cagaaaccgg tttgttcaag
     1321 ccttgtagaa ttggtccgat tgcttcaaag ttaccgaaac gctgagcaat tttatagccg
     1381 atatttcctg actgcaattc tggaaataca aagactgttg cttgaccagc tacttctgaa
     1441 tctggcgctt tcagttgtgc aactgaagga acataagaag catcaaattg caattcgccg
     1501 tcgatcatgt attgtggcgc tagtttttta gcgattttag ttgcttcaac tactttgtct
     1561 acttctggcg atttcgctga gccttttgtc gagaagctca tcattgctac ttttggttcg
     1621 atatcgaaca attctgctgt ttttgcagaa tctacggcga tttcagccaa ttcttgcgca
     1681 gttggattca cgttgatcgc acaatccgaa aagacatatt tttcttgatc tcttccacgt
     1741 accatgatca tcgctccgct tgtccgactg atgccaggct ttgttttgat gatctgaagt
     1801 gctgggcgaa cagtgtcgcc agtcgaatgg atcgctccag agaccattcc gtctgcaatc
     1861 cccatatacg taagcatcgt accaaagtaa ttcacatctt ttaaaatctt ttctgcttgt
     1921 tctttggatg cctttccatt tctacgttca acaaaagtat ccaccatttc atcccactta
     1981 tcgtaattat cagggctgat gatcgtaaag ctagatgttt tgattcctct ggcatgcgct
     2041 gcatttctta tttcttcttc gttccctact aaaatgggtt ccacaagttc ttctgctttc
     2101 aaccgggcag ctgcgcctaa aatacgagca tcagtagctt ccgggaaaac gattttgatc
     2161 ccacggcgaa caattttaaa tcttaaacta tcaaacaatt ccacaagatg acctccagac
     2221 gtttttatat gtattatcat acactattcc agaaaaaaag aacagtttat tttatctgta
     2281 tcagtcaaaa atgctggaaa aattaagttt ttcacataca aagcgaactg cactataaca
     2341 ggaaagcgct gttttattac actgtttcag gcagttgcca atcaatcggt tcttctccta
     2401 acgcaaggag cgcatcattc gttttcgaga agggtttgga accaaaaaag ccacgacttg
     2461 cagataacgg actgggatgt ggagatgtta tgatgatatg gcgactctta tcgatcattt
     2521 tgattttttc ttgtgccggt tttccccaca agataaacac gattggtttt tcccgttcat
     2581 ttaatttttc aatgatcgtg tctgttaatc tttcccaacc tttcccgcga tgagaatacg
     2641 cttgcccttc ccgaacggta agaacggtgt tgagtaaaag gactccctgt ttagcccatg
     2701 acactagatt tccatgttga acaggtgcga ttccaagatc atcataaagc tctttataga
     2761 tatttcttaa cgatggcggg atctttacgc caggttgcac agaaaaagat aagccatgag
     2821 cttgattttc tccatgataa ggatcttgtc ctaaaatcac cactttaact tcttcataag
     2881 gagtcaattc caatgcttca tagatatgat acatatctgg atggatcttt tgggacgcat
     2941 attctttttt taaaaattct cttagatgca gataataatc ttttgaaaat tctgaggaca
     3001 aaacttcttg ccaactgtta tgaatgattt ctttcaaaaa ctccacctcc taataaaaag
     3061 aataactcac attttcttca atcacaaggg acaatcttca ccctcagcac ttgattaata
     3121 tggtaaacta ggaacaagaa gcagaaagga acgataatca ttatgatcaa attaattgca
     3181 tcagatatgg acggcacatt attagatgcc catatgagtg tttctcctga aaatgccgag
     3241 gcgatacgtt ttgcaaatga tgccggtgtt gagttcatgg tcgctactgg tcgaaactat
     3301 caagaagctc gcgcagcttt agatgaggta ggaatcgatt gtgcgatgat cactttgaac
     3361 ggggcacagg tatttgataa agaaggaaat tcattgttta ccgtgtcgat tccaaataat
     3421 caagcaatct ctgttttaga tattctagat gctaacggta tttactatga agtagcaacc
     3481 aacgatggtc tttattctga aaatcaagcg aaacgaattg aaagttttgc ttccatggtg
     3541 gctacccatt tacctcacct tacgtataaa atggcgattg ccatggcctc agctaagctg
     3601 gagttattgc acatcacata cgtggacagt attcgtgcta ttttggaaga aaagaatctg
     3661 gaagttttaa aaattatttg ttttcataca gaagggccag ctgtattagg tccagttgga
     3721 aagcagattt ctcttttagg cgatctagcc gttacttcct ctggtcaaaa caatatcgaa
     3781 gtgaaccata aaaacgctca aaaaggaatt gctgttgccc atgtcgctca cgagcgtggg
     3841 atttctttag aagaagtcat gacgattggc gataacttca atgatgtcag catgctgcaa
     3901 gtagcaggag tcagtttcgc aatgggaaat gcagaaatcg aagtaaaaga ttatgccaaa
     3961 tatacaacgg acacaaacct tgaatctggc gtaggaaaag ccatattgcg cgcgattgca
     4021 gaagacttgt agttttatct agaaaagagg gatatagatg tcagaatttt tcattcagga
     4081 aaagcagctt ggacgtgcaa ctcggacaat tgtcaaaaac gaaaaaggac attccctgtt
     4141 tttgatggtt ggtcgctggg gaacaaaggg cgatgcattg tctctttatg ccatgaatgg
     4201 cgatctcgtt gctcacatca agcagataag cctgacattc ggtacgcgtt ttgagctgta
     4261 taaagagttc gaaaaggtgg gaaccatgca gaaaatattc aattggcccg gtgactttta
     4321 ttatattcgt agacttcact ggacagtcca aggtgatatc tataatcatc aatatcaaat
     4381 ccatcatttc aatcagcaaa tcatgaaaat ggataaagca acactttttt caggagatta
     4441 ctatgtattg gatgtctcaa atgatgaaga tgctccagtt tgtatttgta ttgctgcggt
     4501 tttagattac tggctctacc gtaaagacaa aagtcgcgat aagcctgttt cttaccgact
     4561 taacttaagt taaaaagatg tacctgaaat gcggaaatac gcatttcagg tacatctttt
     4621 tagatattct tttgcatatg atcataaaga tgaccactga tcatccgctg accgacaact
     4681 ttgaagtcat ggcgttcgta taatttttta gcctttggat ttttttcatc cacacttaga
     4741 ccgatgacgg agcgattagc tttcttcgcc aatttaggta atgcttctaa taatcgcgaa
     4801 ccgatgcctt gtcctctaaa atcttctgat acactgatag agtccaaata ccattcgttg
     4861 ggaaacgttt ctttatcaat gaacaaacgt gtagacgcat ctagctgata tttctctaag
     4921 ataggtacca gtggctgatc gatgattgat tcttcttcat ctgtataacc gtaagcaact
     4981 cctgcaactt taccgtctat ttcttctacg atacctcgtg aataactgta acgataattt
     5041 ggatcagtga ttgcttcttc taaaatctct aaagtctttt gttcccctgt ctgcttcaaa
     5101 aatggcagtt ccatatcttt tagaatcact aagatcaatg gggcgatttc tggagcatct
     5161 tcttttctcg caaaacgaat catagtttcc tctccttttc tccattctaa catgcagaaa
     5221 aagaaatcgc aaaatcgtta ttttaaaaaa tcttcaatct tttcagtaat gaaataacca
     5281 tgagtaacga ttcttgccgc ttcagttcgg caataagggc agacgactgg catttcttta
     5341 tcattatgtt cgactacatt attgacttct tcgtatgcaa atttttttcc gcaattaggg
     5401 catttcattt tttccatgtt atctcaactt cctttcatct ttactctctt agcttactct
     5461 tatattgtaa aaaagagaag caatatcctt cttctatgca gatatccata cctgacggtc
     5521 ctgttctcca tctattaatc ggactttata ataaggacgt tcatctttga ctagaagtgt
     5581 ccactctttc acttttgctt ttttcccgta gtagctgact gcttccttca tcgctgcttg
     5641 cggttgtttg aactcttccg ttttttctat atcaaaaaga tgttctgcgg ccttttccgt
     5701 gtcatttcgt acggttgttt tcccagtgga tggattgact gctatgactt gtccttcaca
     5761 aaaaaactgg tattcatatg ttgcattcgt aggattctcc aaatcttttt caactgcaaa
     5821 atgaatagca gatatgttat gttcttttgt ttcgtttttt acgattgctg ctgctttttt
     5881 caatgagatc cgatattcct ggtcactaat atcatccatg tgaaaagcgg cattgagcag
     5941 tcccgtcata ccggtaatcc ctattacgat gagggcagca acaccaaaca tcattttttt
     6001 cattcttcat tctcctgttt ctttttttta aagaatatca agaaatgaac gaatgaaaaa
     6061 tcgaatgaaa cgaaaaaaaa gtgacaattt tgttatcttt attcgtgcag ttctaatggc
     6121 aatccgtctg gatcaaagaa aaacgtcatt ttttttcctg taaaggtatc ttctctaatc
     6181 ggttctgatt cgatccctaa ggactgcaat tctgcaattg tttcttcaat atcgtctact
     6241 ttgaaagcaa gatgacgcaa gccacaagct tctggatagt ttggtcgttg tggcgcatta
     6301 gcaattccaa aaatctccaa ttcagcactt cctagtttca gatctaattt atagtcatct
     6361 cgttctttgc ggtaattttc tctgattact tcaaatccta atttattaac ataaaaatca
     6421 cgtgatttct gataatctga aacaatgatg gctatatgat ggatcgttgt aaggttcata
     6481 tgagcactcc tattcttcgt ttaagctcgg atctatatta atctccagtt ccattgtaaa
     6541 tgtgtcagat tgaaaaaaat agctgttttt cctttttata agaaaaaagg caaaaaaaac
     6601 agaaagtagg tcctccaaga cactcttcct gttcccaata taataaatga aagtaagaaa
     6661 ttatattttc aagaaaacaa acagaaaatt atcataacac aaattattta taattgaaag
     6721 actagttttt tttcaagaaa agcatacaat agattgtgtt agtaaataca gaacacacta
     6781 catttagtgt cttaaggagt cctcatacat gaagtcatat tttcagtttt tagctcatca
     6841 gctaaaaggc tggccgcaac aaaattatta tcttttcttt ttcagtcttg gctgccaggt
     6901 catgactctt gtcaatagtc ccatcacttg gctgtccgtt attacattta tcggtaccac
     6961 gcttggtgta ttgtgtattt tatccatcaa tgcggcaaaa tcagtcaacg gtattttagg
     7021 tattttgtct gcgatttgtt ttattatcgt tgggttttca gcaaagaact atttaagtat
     7081 cggtgaacag attgcttata tgatcacatt ggatatcccc gtattgttaa gtaaagaatg
     7141 gaatcataat atggcttcaa aaatccgaaa attcactgga aaaagctggt ttatcgcaat
     7201 catcagtact ttccttgtct atcttgtttc aggattcctg attggtcgtt atacagatga
     7261 tcctcggccg tgggtagatg ccattgcttt ttctattagc ttgactgctg gtatcatcag
     7321 tttcttgcgt tacaacaacc aatattactg gtggttagct tcagggcttg ctcaaatggt
     7381 cctttggtat atttcattta cgcatggatc tgcaagtttg gcgatgttcg tgaacagctc
     7441 tgtttacttg ctgaatgatg tccttgcatt taccgtttca ccttggtata accaaaaaga
     7501 acgtgaacgt atgcaaaaaa tggaagaaga atactacaac gcacaacaac acgcataaaa
     7561 aaactgagcg tgggacaaaa gtaaagaaca cttttgtctc acgctctaaa cacgtataaa
     7621 cggcggaa
//
LOCUS       DAYYCY010000119         7553 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000119 DAYYCY010000000
VERSION     DAYYCY010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7553
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.119"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            218..1756
                     /locus_tag="ACHAZB_002101"
     CDS             218..1756
                     /locus_tag="ACHAZB_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HFM8614737.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            1753..2718
                     /locus_tag="ACHAZB_002102"
     CDS             1753..2718
                     /locus_tag="ACHAZB_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8614738.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            2705..4027
                     /locus_tag="ACHAZB_002103"
     CDS             2705..4027
                     /locus_tag="ACHAZB_002103"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFM8614739.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      4090..4194
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4268..5011
                     /locus_tag="ACHAZB_002104"
     CDS             4268..5011
                     /locus_tag="ACHAZB_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8614740.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            5026..6018
                     /locus_tag="ACHAZB_002105"
     CDS             5026..6018
                     /locus_tag="ACHAZB_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8614741.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            6030..6767
                     /locus_tag="ACHAZB_002106"
     CDS             6030..6767
                     /locus_tag="ACHAZB_002106"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8614742.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            6757..7479
                     /locus_tag="ACHAZB_002107"
     CDS             6757..7479
                     /locus_tag="ACHAZB_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFM8614743.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
BASE COUNT         2462 a         1297 c         1640 g         2154 t
ORIGIN      
        1 aagtgacaat ttcgtataga tttttatggt attcgtagtg aaaaaacgag aaccaaaaag
       61 ttgatccgac tttgttgctt ggattctttt tttgtcagca tatttagagg tatttttttc
      121 ttttttttgt tcgtgctgta aggttaaggc aagacaagtt agttgggaaa aaggaaagaa
      181 atgtttatac ttaaagaata acagaaagga aggtcgcatg aataatttta tacaaacatt
      241 aagtgaaaga aaaggcgagt tgctggaagc tgcatttcaa catctgtcca tttctctcat
      301 ttctttgctg attgcagcac tgattgctat tcctttagcg atttgggcag ccaatcataa
      361 aaaaatggca gaagtcttat tgcagatcac gggtgtgctc caaacgattc cttcacttgc
      421 cttattaggt ttacttatcc catttgtagg gatcggaact gttccagcat tgattgcact
      481 tgttatttac gctttgcttc cgattttcca aaacacgtat attggtctag ctgaaattga
      541 cccgtctatt gaagaagcag cggtagcatt tggtatgtcc agaatgagac gcttgctgaa
      601 agtagagctt cccatcgcat tacctgtcat tatctcaggt atccgaactg ccttagtttt
      661 aatcatcggt acggctactt tggcagcatt gatcggagca ggtggattag ggacatttat
      721 tttgttaggt attgaccgaa atacacctgt tttgacattg attggtgcga tcagttctgc
      781 cttgcttgca attatattca gcggactgat tcgctggttg cagcataaga aacctcgata
      841 ttccatcatt actttagcag cagtggtgtg tttatttggc ggcgtttctt tatatcagtc
      901 tgccttgttt aaaaatgaaa ccatcacgat tgctggaaaa ttaggagcgg aacctgaaat
      961 cctgatccaa atgtataaag aattgattga agacaacaca aaaacagaag ttgaattaaa
     1021 accgaatttt ggcaagacga gttttttgtt caatgcactg gatcaaggtc aaatcgatat
     1081 ctatcctgag ttcacaggta ctgtcctaga aagcttggtg aaggtacctg aagcaatgaa
     1141 aagtgctgat ttgacaaaag aaaaaacata tgacgaagca gaagaattat tgaataagca
     1201 attcgatatg acattattaa aaccaatggc ttaccaaaat acgtatgcct tggcagtcaa
     1261 gagatctttt gcgaacgaac atcaacttaa aaagatttcc gatttggcag ctatctctga
     1321 tcagatcaaa gcgggattca cactggaatt tattgatcgt tcagatggat ataaaggaat
     1381 ccaagaagca tatggcttga atttttcaag tgtacaaagc atggagccaa gtttgcgtta
     1441 ccaagcaatc aataatggag atgtcaatat tgtggatgcc tattccacag atagccagct
     1501 caaggagtac gatctagtga ctctagaaga tgatcgaggg ttattcccag cttatcaagg
     1561 cgcaccgctg atgaaaaaag attttgctaa agaacatcct gaaatcgtta aagcactgaa
     1621 taaattaagc ggaaaaataa cggaggatca aatgattgat atgaattacc aagtcaatgt
     1681 cgaaaagaaa aagccgacag aagtagcacg aatcttcctg aaaaatgaag gattagtaaa
     1741 ggaggaagat cagtgactaa attaatcgag ttcaaacatg tgcaaaaaaa gtatgatgga
     1801 aagtatgtca tcgatgacct aaatctggca atcaaaaaag gagaaatctt tgttttggtt
     1861 ggtccttccg gtagtggaaa aacaactact ttgaaaatga tcaatggatt atctaaacca
     1921 tcagcaggag acatctactt caaaggaaaa tcgttaaatg aatataatct gcagaaaatg
     1981 cggtggaata tgggatatgt gctgcagcaa atcgctttgt tcccgaccat gacagtcaag
     2041 caaaatattg aagtcatccc tgaaatgtta ggttgggaaa agcagaaaag agcagaccgt
     2101 gtagatgaac ttcttcaaaa agttggctta tctcctgata tttatcgaga tcgtatgcct
     2161 agagagttgt ctggaggaga acagcaacgg atcgggatca tccgagctat tgctgcaagc
     2221 ccagatgtga ttttgatgga cgagccattt agcgcattag atccgatttc aagaaactca
     2281 ttacaagaat tggttttgtc cttacacgaa gaattgggca cgaccatcgt tttcgttacg
     2341 cacaatatgg aagaagcaat caagctaggt gatcgtattg catttatgaa ggatggagaa
     2401 attatccaat gtgacacacc agagcagtta ttgatgaatc ctaaaaacga ctacgttcgg
     2461 catttctttg atgaaccgaa acagacgaaa gagtggcgag tagaagattt agtcgtcaat
     2521 ggatatttct taaatgaaat accagaaaat gctcgtcaac aagtttgctt tgatacgcct
     2581 atgaaagaag tatattcgct tctttcggtg tatccaagca ttgtgatagt agaaaagcaa
     2641 agaagtattg ggtcgctgac aagtaaagaa atctttgctt ttttaagtcg agaggaggaa
     2701 ggaaatgaaa acatatgatg caatcatcat cggttcaggt gtgagcggct taagtgctgc
     2761 ttatggattg aaagaagcag gtaaaacagt actagtggtc gaagaagatc tatggggcgg
     2821 gacttgtcct aaccgtggat gtgatcctaa aaaagtcttg ttaagtgcag tagaggcacg
     2881 taatcgggta aaacaattga gtggaaaagg ttttaacgag atcccaactg ccaactggga
     2941 agaacttcaa aaattcaaac gaacatttac ggatccagtc ccagaaagcc gaaaaaaaca
     3001 attagcagaa gctgaaatcg atcatctttc aggaaccgct cgttttttgg atgattcatc
     3061 tattgaagtc aacgaagaag tcttccatgc ggattactta gttttggcaa ccgggcaaag
     3121 acctacgatc ttaccagtgg agggaaagga atacttgaaa actagtgcag actttttatc
     3181 attgcctgta ctgcctaaag aaatcatttt tatcggtggt ggttatattg cttttgagct
     3241 agctacgatt gccaatgctg cagggagcaa agtaaccatt gtccatcata atcaacggcc
     3301 attaaaagaa tttgaggcgt ctttggtaga ggaagccgtc catcaaatgg aagcatctgg
     3361 cattcagttt gcttttggtg ttgaaaccca aaagatcatt tcagaaggta ctcgctatcg
     3421 tttagtcgga aaagagacgg aattagtagc agatatgatt ttttgtgcga ctgggagaca
     3481 gccgaataca gaatctttag cacttgagca agcgaatatc gtctttgata aacacggaat
     3541 tgcagtcaat gattatctgc aaacgagcaa tccaaaaatt tttgcgtgtg gagatattgt
     3601 ttcaaggaaa accccaaaat tgacaccagt cgccactttt gaaggaaatt atgtagctaa
     3661 gcggataacg gatgcaacaa gcgaacctat caaatatccg attataccta cgatcgtcta
     3721 cgccagtcct aaacttgctg aagtaggagt tacaaaaagt cacgcctctt cgtcagatca
     3781 agtggtagag atggatctta ccagttggtt cacctatcat cgtgtcaatg aaccagttgc
     3841 taaggctgaa ttgacctttg accagcaaaa ttatttgatt ggtgctgcag tgattagcga
     3901 gcaagcagac gaattgatcg atgatttgac actcgtcatc aatcaaaaat tgactaaaaa
     3961 agagttggat agctatatca tggggtatcc gacattggca agtgatctct cctatttatt
     4021 gaaataatca gcatgataac taaagcgctt gttgcttttt ccttcttgtg ctacacttta
     4081 aaaaatgaat acctgtttct ggggatgtcg tagacgacat gagagtaagt tgtgaactta
     4141 gacccattga acctgtcagt taatactggt gaagggagaa acatagcaga cgcttgaagc
     4201 gggttactat gtcgtgccca tttcaagcgt tttttgttat tcatattaga agaaaggagg
     4261 taacaaaatg aagaaaaatc tttatccagc agtcagtatg gcattcattt taattatttg
     4321 gcagattgtt gtcatagttt ggaaaatacc tgcctttatt ttacccggac ctgctgctgt
     4381 attttctgca ttagcggcag atgcacaagt attactttcc cattctgtca cgacattaaa
     4441 tgaagcagtg cttggtttgg taatagctag cgttctttct tttattactg ccttggcaat
     4501 ggattattgg aagtttttag aaatgtcttt ttatccgtta ctagtgattt cacaaatgtt
     4561 gccaatcatg gtgttagggc cattacttac cctatggttt ggtttcggta tgcttccaaa
     4621 agtgatcctt gttgtcttga taagctattt tccgatcgta gtttcttttt cggatacatt
     4681 gaggaaagta tcaaaagagc aaatcatctt tttgaaaacg atgggggcag atacagggaa
     4741 gatttatcga atatataaaa taccagcggg tttatccggc ttcttttccg gtttaaaagt
     4801 ggctgctact tattgtgtca gcggagcagt cgttggagaa tggctaagcg ctcaagcagg
     4861 cttagggtat tacatgatac gcgtaaaaaa cagctaccag atcgataaag tttttgctgc
     4921 gatcatttgt gtcattctat tgagtttatt gctgaatggc gcttgttcat tattgaaaaa
     4981 agggtattat cgtatgcttt atatttttta agagaagggg atcaaatgaa aaaattatgg
     5041 cttttattac cgctactgtt gcttttatcc gcttgtggca ctgcaaaaga aacaagtagc
     5101 aaacaagaaa taaaggattt gaaaaaagtg acacttgttt tggattatgt accgaatact
     5161 aatcatactg gcatctacct agcaaaagaa aaaggctatt ataaagaggc tggtttaaat
     5221 gtccaaatca tcgaaccagg agataattcg acaagtattg gtctagtagg tgcggacaaa
     5281 gcacaatttg gtgtaagtta tcaagaagat gtcacttatg ctcacgcaga tggacagaat
     5341 ataccagtca aagcaattgc tacagtgatc aaacataata cttctggatt cgccacccta
     5401 tcagacagta acatccattc accaaaagat tttgaaggaa aaacatatgc aggttggcaa
     5461 agtccaagtg aagaagctgt cttaaaagct gtgatggaaa aagacggcgg ggacttttca
     5521 aaactgacaa tggtaggaag caatggagaa gggccagaaa gcttaggaaa atcctcagat
     5581 atccaatggt actttgaagg ttgggatatg atcaaagcaa aagaagcggg tattgaacta
     5641 aactatatcc cattgaaaga attagatgaa cggttagatt actatactcc ggtaattatt
     5701 acgaatgatc aactgatcaa aagtgatcca gaattggtac agtcttttat ggatgccacg
     5761 aaaaaaggct atcaggaagc tattaaggat ccaaatgaca gtgctaaact gttgcaaaaa
     5821 tatgcaaaag aaaatgatcg gacgttttta gaagaatcac aagcattctt gtcaaaaaat
     5881 tatacggatg atcctgagaa ttggggattg atggaagaaa aagtttggcg caattataca
     5941 gcttttatgc aggaaaacgg attgatcaag caagatgtac cttcgcaaga actattcact
     6001 aatcagttta taaaatagga gtgatagata tgtcaatcca aatcagccac ttgaatgtag
     6061 tcattgaaaa cctgaaaata ttagaagaca tccatttcac tatccctaaa gataccttta
     6121 catcgattgt cgcgccaagc ggagccggta agtcaacatt gctgaaaact ttaacaggag
     6181 tccagcctat gacttctgga acgatcaaag tagacgatca aaaagtaact ggtctccata
     6241 cagcatttag ttatatgccg caagaagata tgttacttcc ttggctgagc gtctatcaaa
     6301 acgttacgtt atatcaaaaa atcaatcatc taacaatcaa tgaagaacaa gtaagggatt
     6361 atctggatat ctttgggtta aacggctatg aacatttttt gccggaacaa ttatctggcg
     6421 ggatgaagca gcgaacggcc ttgttacgaa caatcatgaa tccttcttct tacttgcttc
     6481 ttgatgaacc atttggagcg ttagatgcta tgaccagagg gcagatgcaa gactggatgt
     6541 tgaagctgcc taaaaaagca aagcggacta ctttacttgt gacgcatgat atcgaagaag
     6601 caatttattt atcagacagg attcttgtgc tttccgcccg tccggcacat gtgattgctg
     6661 agattcaagt gcctgaaaaa cgacggtcac gtgaatggct actgcagcaa tcagagttga
     6721 agcaaacgat ttatcagtta ttggcaggtg aattagatgt taagtgatgc tcattgtcat
     6781 ttagatggga gtcagagttt agcactcctt cagcaggaac acacgatttt gacgatcatc
     6841 aattgtgatt cgccagaaga atggaaggaa aacagacagc ttgcagcaag taagactcaa
     6901 gccttaagtt acggtatcca tccttggaaa gcagatagct atacgtttga acaagttgag
     6961 ccttttttga aaaaagcaag gatcatcggt gaaatagggt tagataatat ttggacgaat
     7021 gtaccgatga ctacacaaaa aaaagtattt gagcgacagc ttgcttttgc agccataaat
     7081 gagaaacctg ttgtactgca cacaaaagga tgcgaaaaag agatcttaaa ttatatccaa
     7141 gcttacccta atcactattt gatccattgg tattcttctt tggactatca acaagactat
     7201 attgatttag gctgttattt cagcattggt cttgatttga aaaagaatcc agcagtctgg
     7261 caactagcta aagcagtccc aatcgaccac ttacttattg aaacggatgg actacatgca
     7321 gcagagtggg ctttaggggg gattttttca gaaaaagatt atgtctcact gcttgaagaa
     7381 cagttaaaga caatcgctga gatcaaagag ctatcagttg aagaagttaa aaaaaatact
     7441 tatcaaaact tggaacgttt cattagatta ggtaaataaa aaagagatgt gacacaacta
     7501 gtgtcgcacc tctttttggc tttctttgta taaaagtaaa ctgtttgatt aat
//
LOCUS       DAYYCY010000120         7490 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000120 DAYYCY010000000
VERSION     DAYYCY010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7490
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.120"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(263..1711)
                     /gene="cls"
                     /locus_tag="ACHAZB_002108"
     CDS             complement(263..1711)
                     /gene="cls"
                     /locus_tag="ACHAZB_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFM8614744.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            complement(1863..2747)
                     /gene="ftsX"
                     /locus_tag="ACHAZB_002109"
     CDS             complement(1863..2747)
                     /gene="ftsX"
                     /locus_tag="ACHAZB_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFM8614745.1"
                     /translation="MIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFMAVI
                     MNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELKKIQ
                     NEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSDRIF
                     QIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRWPFF
                     LEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMVGIG
                     VIIGSLGSIISMRRFLKF"
     gene            complement(2740..3426)
                     /gene="ftsE"
                     /locus_tag="ACHAZB_002110"
     CDS             complement(2740..3426)
                     /gene="ftsE"
                     /locus_tag="ACHAZB_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFM8614746.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            complement(3531..4632)
                     /gene="prfB"
                     /locus_tag="ACHAZB_002111"
     CDS             complement(join(3531..4559,4561..4632))
                     /gene="prfB"
                     /locus_tag="ACHAZB_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFM8614747.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            complement(4758..7292)
                     /gene="secA"
                     /locus_tag="ACHAZB_002112"
     CDS             complement(4758..7292)
                     /gene="secA"
                     /locus_tag="ACHAZB_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFM8614748.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPHHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
BASE COUNT         2022 a         1655 c         1297 g         2516 t
ORIGIN      
        1 taaataacaa aataaattct cataattgta aaaaaaacta tctcattaaa agtttctatg
       61 ctatactggc tttgaaatgt gtctctttta atagcatata ttagcttatt tcttgctttc
      121 cccaaaaaac aagagatacc ccaaagttaa tttcgctcat gcgagacgct ttctccattt
      181 ctgacgtact ctcagaaatg gccccgttag aaaaggacac gagatctcca tctgttgatg
      241 gttctcatgt cctttttatg ttttaaagga gcggcgaaat caatcttgct aatccttctt
      301 tcatcttgat gattattcct cggttttcat acatttcttt tgtgagatgc gtagaaattt
      361 tcgagtcttc aaaaaaagct tttcttacac gtgaagccat tcgttcatca taaaccagtg
      421 tattgacctc aaagtccaag cgaaatgaac gaacatcgat atttgctgag ccaactgaaa
      481 caatgccacc atcgataatc atcgtctttg catgaataaa tccattctca tatgtttcga
      541 tccttgctcc atactcgatt agctctgcag cgaaagaata cgttgcccag taaacgagaa
      601 gatgatctgg tttgttaggt atttggatat gaacttttac gcctgaaaga agagccaact
      661 tcagtgcttc atgaatcgat ccatccggta tgtaataagg cgtttggatc aatatctccc
      721 gtttggctaa cgaaatcatt ttcaaataag tcattttgat ttgttcatgt tcggtatctg
      781 gtccgcttgt cacgatttga acagctatat caccagtaga gtgaatggac ggaaaaaatt
      841 tgggagaata gcctacttct tttgtatgct gagaattcca gtccatcaaa aaacggtttt
      901 gcaaggtata caccgcttct ccgtaaatac gcaaatgatt gtctcgccaa taaccaaatt
      961 tctttaccaa tcctagatat tcatttccaa cattgaaccc acctgtatat ccgattttcc
     1021 catcaataac tacgatttta cgatgattgc gataattgat ccgaggatta atatacggaa
     1081 caaatagagg aaagaaaaat gcgacttctc cgcctgcttt tttcaaattc tgaaaaaagc
     1141 tggaagaaac ttgtgtggaa ccccatgcat caagaaggac tctgactttt acccctcgtc
     1201 ttgccgcctc tgtcaatgca tctcgaactt cttcacctaa tgcatcactg cgataaatat
     1261 aatactggaa gtggatatgg tcttctgcct gcttgatatc ttcaatcagc gcatcgaatt
     1321 tttcacgacc atctgtaaat aaagtgattt catttttagt cgtatagagc gtactttcaa
     1381 ataccgtgag catatagatc aattgtttca catctacttg tccagtaggc ggatgaggat
     1441 aaagccctcg catcaatgcc tgtttgtctt cttcaatttc caggttctta ccgattttcc
     1501 cttggatctt caaatcaaag attttctctt tggaaatccc tcttcctaaa aaaatatata
     1561 aaataaatcc tacaactgga atgaacatca aaacgagcac ccatgcccat gtttgagcag
     1621 tttcctttct ttctcgaaaa acgatgataa aggaaagcaa aatattcata atcaaaagga
     1681 tcgtaaaaaa attgtcaacg ataattttca cggcttcacc tcttcttcta ccaacttaag
     1741 aatacccaag ttgtttttta aagtaaaggg aaaaaagccg cttccttcta ttctcctagc
     1801 caataaaaaa agaaagctgg aacgaaactg tccaactttc tcattttatt tatattgtgg
     1861 tattagaatt tcaagaaacg tctcatagaa atgatcgagc caagtgaacc aatgatcaca
     1921 ccaatgccga ccattaaaag attgattttc caaacgatat tttgtggatg aatcaatgaa
     1981 taatgggaac gtaataatga tggattgatc aattggaaaa accatgcata accaaatgtg
     2041 atgatcaaaa tcggaagaat cgcaccgatc agaccgatcc agccaccttc aaggaaaaac
     2101 ggccagcgaa tatatccatt ttttgcaccg actaaacgca tgatttgaat ttcacgctgt
     2161 ctagacaaaa tcgtgatccg aatcgtgttc gaaatgagaa acatcgctac gaacagcagc
     2221 aatgccgctg caactaatcc ccatgtccga actgcaccag cgatttggaa aatccgatct
     2281 gaggaagctc caccgtaatc agaacgagaa acattcttca aatctgctgc ttcttctgct
     2341 acttgttttg tataagaagg atcagtagca cttactacat acacatcata taacgggtta
     2401 ttgtcacctt caaacaattt ccaggaatca cccatttcgt tttggatttt tttcaattcc
     2461 tgttctttgc ttgaaaattc aactttttta acatgatcta agtcattcaa ttctttttct
     2521 agctttttca tctcttcgtc ttttgtaccg atatctacga atacagagac atcaacgttt
     2581 ccttctatat cttgagctaa tttagttgcg ttcatgataa ctgccatgaa tacaccgact
     2641 aaagtcaatg tgatcgttac cgcactgact gaagcaactg tcatccagcc gttacgtttc
     2701 aaactcttga tactttctaa taaatggcgg aaaaaggttc taatcatcgt atccatattc
     2761 cccttccgct tgatctcgaa taatgcggcc gttttcgata gcaattactc ggtgccgaat
     2821 cgtatttacg atcgtactgt tatgtgtagc catcacgaca gtagttcctt gggcattgat
     2881 ccgatctaat aatttcatga tttcccatga gttttctgga tccagatttc ctgtcggttc
     2941 atccgcgata agtactttag gcgtattcac tattgcccga gcaatagaga cacgttgctg
     3001 ttccccaccg gataattcgc taggaaagac tcgaactttg tgtttcaaac caacaaggtc
     3061 cagcacttcc attactcgtt ttttgatatc ccgaggtttt ctgccgataa cttgcatagc
     3121 gtacgcaaca ttttcatata ctgtttttct tggtaataat ttatagtctt ggaacaccac
     3181 gccgatttct ctgcgtagat aaggaacttc gcggtttttg attgctatta gatcatgacc
     3241 tgcgacgttc aaagttcctt ttgaagcttt ttcttctcga tacatcaatt tgatgaatgt
     3301 agatttaccg gctccagacg gaccaaccac atagacaaac tctccttgtt cgatgacaat
     3361 tgagagattg cggatagcgg tcgtaccatt tgagtacttc ttcattacat ccttcatttc
     3421 aatcatggcg ttctctccat atctttttca gtctttagct attatagcat ggcaatgttt
     3481 caacatgctt gcaaaatctt acaattttat ttcaatttaa caatgggttc ttattgtaga
     3541 tgcattttta aatagccgtc aataaataaa tcaaggtcac cgtccataac tgcttggaca
     3601 tttcctgtct ctacgtttgt ccgatggtct tttaccatag aatatggatg gaaaacataa
     3661 gaacggatct gtgaacccca gccgatttct ttttgttctc ctcgaagtgc agctgcttct
     3721 tgttctttct tttccacttc taattgataa agcttagctt ttaacatccc catcgcttgc
     3781 tcacggtttt tcaattgtga acgttgtgcc tgactagcta ctactgttcc tgtaggcaag
     3841 tgagtgatcc gtacagcaga ttctgtttta ttgatgtgct gaccacctgc accacttgct
     3901 cggtacgtat cgattttcaa atcatctgga ttgatttcta tatcgatcgt ttcatcaagc
     3961 tctggcatca catctaccga acaaaaagaa gtatgacgtc gtttcgctga gtcaaaaggc
     4021 gagatacgta caaggcgatg aacacctttt tctgatttca gataaccaaa tgcgttgtgt
     4081 cctttgatca gtagggtcac actcttgatt cctgcttcat cacctgcttg ataatctagt
     4141 gtctctactt gatacccatg ctgctctgcc cagcgtgtat acatacgtaa aagcatactg
     4201 ccccaatctt gggactccgt tccgccagcc cctggatgca attcaacgat ggcgttgttt
     4261 cgatcataag gaccatctaa caacatagat aattcatatg tcttcatttt ttcatctagc
     4321 gcattgatac gtgtttctgc ttcttcttgc atggctgcat caggttcttc ctgcaacatc
     4381 tccaccatca attcgatttc ttccagttct tcagcaagag aatggaattg atcataagtt
     4441 tctttatttg cattgttttc actgatcact ttttgagcag cttcgctatc atcccaaaaa
     4501 ccaggttcag ccatttttgc ttctgcttcc gcaatgtctt cttccagctg gtctaagtca
     4561 aagagacctc ctgaagcttg tgaccttttc ctgcatttct gtcaggagat ttcgaatttc
     4621 ggatatttcc atgttttttc cttctttcac tatacttttt tcattatatc tagtctaaca
     4681 gaatcagcaa ataacagaaa gaaggtgaga cgaattctta aaaaaagaat ttgtctcacc
     4741 aatcctgctg tcagaaatta gtttgcattt tttccatgac agttcttata ttttttcccg
     4801 cttccacaag gacaaggatc atttcttccg accttgttca catggattgg ctgttttttc
     4861 gcgctagtat tactattttc tttggcatct ccgtcttctg agtgagaagc tgttccttga
     4921 gaaacctgtt cacgctgtac gttttgacgg atctcagctt tcataaagat tcgagtgact
     4981 tcatactcga tggcgcctac catatcttca aacatcttat agccttctgt ttgatattcg
     5041 actaatggat tgttctgtcc ataagcacgt aagccgattg actgacgtaa ttgatccatt
     5101 gcatcaatat ggtctgtcca tttagagtcc acaacacgca agatcacgac tttttcaaat
     5161 tccaacagtt gctctggacc atttagttga gccgctttgg tattaaatac ttcttgtgct
     5221 cgttcgttta gatactcttt gatctcctga gctgtttttc cttcgatatc tgcaagagta
     5281 atactatctt catggacaag agtactgccg gcaaaatcga tgatactttc caaattccaa
     5341 ttttctttct ctaattgtgt atgcgcatct actacacgac tgattgtccg tttgaccatg
     5401 ttcattagtg gcttggataa gcttttttct tccatgatta cttcttgtcg ctgtttatag
     5461 attacttcac gttgttcacg catcacatca tcgtattgta agacgttttt acgtgtgtca
     5521 tagttatttc cttctacacg tttttgtgca gattctactt gtttcgtcag catcttgctt
     5581 tggataacag catcttcctc accgatcttc atccgatcta agaaagattt gattcgttct
     5641 gaaccgaatc gtttcatcaa atcatcttcc aatgacaagt agaactgtga cataccaggg
     5701 tctccttgac gtcctgcacg tcctcgaagc tggttatcga tacgacgaga ttcatgtcgt
     5761 tctgtcccga taactgctag accaccaagt tctcttaccc caagtcctaa cttgatatct
     5821 gttccccgac cagccatgtt ggtagcaatc gtcacagcac ctttttgacc agcattcatg
     5881 atgatctctg cttctttaaa gtgatttttc gcattcagca cttcatgagg aatacgttct
     5941 tgattcagca tatctgataa taactcagat gtttcaaccg caacagttcc gactaacaca
     6001 ggttgacctt tgcggtagcg atctttgata tcttgcacga cagcgtggaa cttgctttgt
     6061 aatgttgggt aaagcaaatc tggacgatca tcacggataa ccggacgatt tgtcggaatc
     6121 tggtatactt ggatgttata aatttctctg aattcttctt cttctgtttt agctgtacca
     6181 gtcattcccg caagtttctt gtacatacgg aaatagttct ggaaagtgat cgtcgccatg
     6241 gttttcgttt cgtcttcgat ctctacgcct tctttagctt cgattgcttg gtgaagtcca
     6301 tctgaataac ggcgtccatc catgatccga ccagtgaact ggtcaacaat cagtactttt
     6361 ccgtcttgga caacataatc gatatctaaa agcatgatat agttggctct caatgcttga
     6421 tccaagtgat gggtcaatgc agtattttca atgtcgtata agttatccaa accgaatgtt
     6481 tcttctgctt tttcgatccc tgcttcggtt aaactgattg tttttgattg tacatcgatt
     6541 ttataatctt cttcttcttt caaacgtttc acgaagttgt ctgctcttgt atataaagct
     6601 gtggattttt ctgcttgccc tgaaatgatc agcggcgttc tcgcttcatc aatcaagatt
     6661 gagtcaactt catcgacaat ggcataattc agcggacgtt gtaccatttg atggcggtaa
     6721 acgaccatat tgtctctcaa gtagtcaaat cctaattcat tattcgtact ataagtgata
     6781 tcacaattat aagcctcgcg tttttcatcc gaagattttg agttgatatt caaccccact
     6841 gttaatccta agaaattata aagttcgccc atttccgtcg agtcacgggt tgctaaatat
     6901 tcattgacgg taactacgtg taccccttcg ccagtcagag catttagata tactggcatc
     6961 gttgcggtca aggttttacc ttcaccagta cgcatttcag ggatatttcc atcgtgaagc
     7021 acgattccac ccatcaattg tacgtggtgt ggatataacc ccaatacacg ttttgctgct
     7081 tctcggacga ctgcaaaagc ttccggaagt aattgatcta atgtttctcc tttttggtat
     7141 ctgcccttga attcagcagt ttttgcctgt aattgttcat cagttaatgc tgccatttgg
     7201 tcagcgtatg cttcaacttt ttttgcaatg ctatctaaac gttttagttc tttcttatcg
     7261 ttttcgataa gtgaacgaat caaattggcc atgcgttgtg taatctcctt ttccttctgt
     7321 gtattatcca gactatgtag actttacttt tcattttatc attagttata gataattgaa
     7381 atatcttaag cgtattaaag taagaacttc ataatttatt catatttcat ctaaaagagg
     7441 aggaaactca aaaaaacttg cggcagctgt cagtagctgc tgcaagtttt
//
LOCUS       DAYYCY010000121         7472 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000121 DAYYCY010000000
VERSION     DAYYCY010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7472
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.121"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            138..1529
                     /locus_tag="ACHAZB_002113"
     CDS             138..1529
                     /locus_tag="ACHAZB_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8614749.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(1602..2249)
                     /locus_tag="ACHAZB_002114"
     CDS             complement(1602..2249)
                     /locus_tag="ACHAZB_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8614750.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(2257..2943)
                     /locus_tag="ACHAZB_002115"
     CDS             complement(2257..2943)
                     /locus_tag="ACHAZB_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8614751.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(2949..4022)
                     /locus_tag="ACHAZB_002116"
     CDS             complement(2949..4022)
                     /locus_tag="ACHAZB_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8614752.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            4244..4918
                     /locus_tag="ACHAZB_002117"
     CDS             4244..4918
                     /locus_tag="ACHAZB_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8614753.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            4923..6263
                     /locus_tag="ACHAZB_002118"
     CDS             4923..6263
                     /locus_tag="ACHAZB_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFM8614754.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(6412..7398)
                     /locus_tag="ACHAZB_002119"
     CDS             complement(6412..7398)
                     /locus_tag="ACHAZB_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HFM8614755.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         2317 a         1384 c         1318 g         2453 t
ORIGIN      
        1 agggaatcac accaattttc ataccaattg gtttcattta tgaagttgat tccttcgatt
       61 aatgagcgtt tcattgaaag aaaagggttg agggagaaaa acatatgatc aggttgttgg
      121 aaggaaacta tagaaagatg gaaaaaatca ttcgaattct gtccatttct gacaagtgga
      181 tgactaaaaa agaacttgcc aaacgaattg gtagttcaga atctacattt gtaaggtata
      241 tcgaagagat caaaatccgc tggggaacgg atgtaacgat tcaatcttca caaaaattgg
      301 ggtatcgtct tgaacatttg aatgtaccag cttatttaaa tgttctgatg gaaatgtcgc
      361 aaagttctac aaacagtcgt ttattaaatg agattattgt caatccgggt aaaacgattg
      421 agtattattg tgaaaaaatt tccatcagcc ggtcttcttt tacacgtaag ttgaaacaat
      481 gcaatcaaat tttagaaagc tatttattaa aagtgatcgt tgaccaaggt tttcagttaa
      541 ctagccttaa aagtgagctc cagttgcgta tctttgttac ctttttcttt ttaagttact
      601 acggaagacg cacgttgcca tttccaatcg aaaaaaagaa gatcaaagaa attatggtca
      661 agaataattg tcagatgaat ttgtttcagg aagaaagccc atatgagcaa tctttcttta
      721 ttatgtattt tgttgtgcat ctcatgagag aatcccaagg aactccattt gaatggaaag
      781 ctggagactc ttatgtacag ttgataaata tcgataaaaa agacttctta atcttgaaaa
      841 atctcttagg aaaattgaag tacagcagct atataaaagt agtgaactat tttgtcaata
      901 cgattttttc agaattttcg gagaaagaaa aaaatgaaat cagtgagcag atacgaaaaa
      961 atatgtccta ctgttcgttt tcatccgctg attgcaaaga aaaaaacgaa gaatatgttg
     1021 ttcaattatt aacgcaaatt tattttttct cccgtatttt cccatacgat acgactcatt
     1081 tgacgatccg tgcaacttac tttgcacagc agatgcaaca ccgccagata cgtctatatg
     1141 ctggatttcg tgcagaactt caattttatt ccactctact gaatcagaat ctgatttatt
     1201 atttatctag ctatatcttt tggatgctta tcggtaatcc tgaaattcac cattatactt
     1261 ctgataaaaa aatactgatt atcagcgact taagtcttcg acatagtcaa tatattgaag
     1321 aatatatcag tgatattctt gctgtgcata aaattcactc tgagacaact gctattactg
     1381 aagatcagct gtcgaaatac aatcttctcg agtatgatct gatcgttacg aatcaaccca
     1441 ttttgaatgc ccatgttcct catatattga ttgatgatag tgtgtcattc gcaaatgaag
     1501 aagaactcat ccgcttattc gagttatgaa taatcaaaaa acagctatgt ataaaccacc
     1561 ctgataaaaa tttcaggaca aatacacata gctgtttctt tttattgtat tccttcagca
     1621 atccaacgag atttatagcg aaagtctttt aacaactctt cttttgtcca attatttgca
     1681 atgagatccg tcaaagtgtt cattacgagg gtcatggaaa agtataggaa actaactgct
     1741 tcttccgacg attgaatgga gaaatgattg attcttaata attccagcca tttttcgttc
     1801 attggagaat cagccgatag ttctctacgt ttggttgtct gctgatcatt tcgctgggtt
     1861 aacaagtata acctttgcca atagatactt tcgtgatcta agtcgcagca ccactctaaa
     1921 tatttttcga tccccaaaaa gaaatgttgt ttgttttcgc taatgaattt taagatatct
     1981 tgatggacta tgtgagaact gcttttgatc gtatacaaat aagcatcttc caaatcttga
     2041 aaatatttat aaaaagcacc gcgcgacatc cccatcttgt caacgatttc tgagactttg
     2101 acttggctga tgtgctgtga gcaaaatatc tccagtaata ggtcatcgat tctctgtttc
     2161 tttgcgttcg ataaattgaa aaaagtttct tttggcatcc acatctctcc tttatctctg
     2221 attatacgtg gatagccctt cattgtcatg aaggattcat tccttctttt tctcttcttc
     2281 tgtcaagtac ccatcttcca ttcgataaac gcgatcactc cattgaatca tacggacatc
     2341 atgagtaacc ataatagtcg ctttttgttt ctcatgagct tctttaacta gtaatttgac
     2401 gacttcatat gcatgatctg tatccaaact agctgttggt tcgtctgcta agatcaagct
     2461 tggctcatta tataaagcgc gagcaattgc cactctctgt ctttctccac cagaaaggtc
     2521 tttaggataa ctatttttaa gtgactggat atctaaagat tgcagcaagg aatcgatttt
     2581 tgttgaatct tctttttttg aagtaacttg gtcaactagt tcaaattgtt cctttaccgt
     2641 taaaaaagga atcaaattag agctttggag aataaatcct atttctttaa agcgtaaatc
     2701 tgctcgtttt ttttctggaa gctcagttag tgagtgaccg ttgatcacta tttctccgga
     2761 agtcggtgtt tgcaatcctc ctgaaatggt aaggaaagta cttttacctg aaccagaagg
     2821 accaatgatg ctgacgaatt ctccttcctt tacataaaaa tcaattgctt tcaaagctga
     2881 gacctttgta tgaccagaac caaatgtttt ggtaactttt ttcatcgtta atattttttt
     2941 catttttatt aacctcctat tgctgtaatt gggtctactt tggttatcgt acgtatcgaa
     3001 aacaatccgc ctagcattgc gacaatgatc aaaatcccac tatatagcaa ccattgtgac
     3061 caaaaaacag caaaaggcat cgcgcttggt aaaatcagac tagtcaaata agcaaacaaa
     3121 agagcaagca gaacgcctac caccccaaca ataaaggact gtgctactaa agatttagcg
     3181 atgaagaaat ttttgatccc ttgtgctttc attactccaa aaatagcagt cttctgaagt
     3241 gtcatcacgt acatgaaaat accgaccacg gcagcaacca ctaagaacaa aaagtatatc
     3301 atcgcattta aggtcatatt ttgggcagaa tagccaggaa tactttcaat gaatgtccca
     3361 atagttaata actgtagacc cttcgatgct ttttctttcg aaattttagc tgaatccttt
     3421 attacgatcc cattgatcgg ctgttcttct tcagaagcaa acggctgttg tccatacttc
     3481 aaagcagtcc acgtatctag ggacgcatag atgacgggag aaactgtgta ataagtttca
     3541 ggaaagatac cgacaatcgt caaaggatcg tcgtaactgc caattttgat cgtatctcca
     3601 attttatagc ctgaatcagc cagattttga gagatgatga tttcattctt tgttttaaat
     3661 tcatgccctt tcgtgagttt tggtaataaa aatgcttctt tcgttgtgcc gaaaactgta
     3721 acattttctt tgttttttcc tttgattgct ccgctgtaca atccgattgg tgctttctct
     3781 cctccttcaa tgtatttcag atctcctctt gttaattgtg aagctgcaaa tacttgattt
     3841 gcatcctccg acaaaacaat ctcttgtgcg tcccaatcat cgattgcttt tttaaactct
     3901 tctgaaagtc cattcgctag tcctgacaac atgaatacga catacgcaat caagaacata
     3961 ataccgacga tcaagccata tcgtaattta gaatagcgaa tttctttgat agctagaaac
     4021 atttgttttt cctccctatg atagattgat agtgacaaaa tgtcacttgt tgaatagtgt
     4081 aactctaaag tgacaatttg tcacttgtgt tgtttttttc ttatcaataa attgtctaag
     4141 acatctttcg ataaaacctt aacatgagtc ataaaaaatt tatttaagcc ttatttatgc
     4201 tttccatgat aaactaaagt gaccaaatca atgaggtggg aaaatgatta aactattagt
     4261 agtagaagac gataaagtat tatccaataa tataaaagaa atactcgctg caatcggcga
     4321 gataacacaa gtttatgatg gtagtgaagg actgttcgag ttggaaagtg gagtttatga
     4381 tcttgcaatc ttggatttga tgatgccgga aatgaatgga tatgaagtgt tagcaaaaat
     4441 acggaaagaa aaaatacaga ctccagtatt gattttgact gcaaaagata gtttggaaga
     4501 taagattgaa ggatttgaac gaggtgcaga tgattatcta acaaaacctt tctatcgaga
     4561 agaactgatc atgcgcgtga aagcattatt gaaacgctcg ctaggtctct ttgatgaaaa
     4621 tgttctgaag tataaggata tcagttgtga tttaaaaagc aatgaagtct tgtataaaga
     4681 acaagttttg cccatacaag gaaaagagtt tgaattgctc gtttattttt tacaaaataa
     4741 gggaatcatt ttgacaaaag agcagatatt tgaccggatc tggggatttg attctgatac
     4801 gacgattaca gttattgaag tttatatgag caacttacga aaacatctgc gaagtactgg
     4861 attggataaa gaaatcaaaa ccttacgtaa tgtcggttat atactgcaaa gggtgtgatg
     4921 caatgaatgg agatattaca cgaaagcaac gtagtcgctt ttttgcgaca aatgtagcag
     4981 catttgcttt tatctttttt cttttgggat tcattacttt acagatatta aaccaatctg
     5041 catacagaga aacagatctg tcattagaga aaataaattt tgattctcga acagtacaat
     5101 tagagataca aagatatcag caaggcgatc cctttttaaa cccgactcaa acggaacaaa
     5161 acagaacaaa catggaacag ccattaatgg aaccatcaaa tgaccgtttt aacactcaag
     5221 tgatcttgtg gtctaaaaat ggtgagatat tgaataaagc agctattggt gggcgtttca
     5281 accaaatcaa agaattaaaa atggatacga aaaatctgaa taccatccaa aatattgaat
     5341 taaatgaaga cagtggctca tctgccctct ctttccgttc gattacgaaa aaagcggaga
     5401 cgaatcaaga tggcgttgcc tacatccaaa ttttagaaaa tacaaatcaa atcaagaatt
     5461 cattagcgac ttttcgcacg attatgattt tgtgtatgat tgttttttgg attctctcta
     5521 ttggtatcag ttattacttg tctagtctaa gcatgcgccc tattattctt tcttggagaa
     5581 gacaaaaaga atttgttgag aatgcttctc atgaattgcg cacaccgtta acgattattc
     5641 aaaatagttt ggaacatctt tttaccaagc ctaatcatac tattattgaa gaatccgaaa
     5701 gcatcgctca agcattgtca gagacgaggc gcctcactgg attgacttcg gatttgctga
     5761 caatcgctcg gaatgattct gatcagcagc tgctttcaaa gcaaatgatc aatacacaag
     5821 aatatatcga acaacttgtt aaaccatttc aagagatggc catcatagat ggaaaagagt
     5881 ttattctcca taacgatgca acattagaag taccaattga tgaaaaaaaa atccatcaag
     5941 ttttggttat tttgctggat aatgcattaa aatatacgaa tccagaagat aagatcattt
     6001 tggattcatc tgttaaccat aaagaatggc tgattcacgt aaagaatact ggcccttcta
     6061 tcagtgaaga agatcaagca aggatttttg atcgatttta tcgggaagat caatctcggg
     6121 cgaaagagac tggcggatac gggcttggct tggcaattgc aaaacaggta atcgaacagc
     6181 atcatggaca gatttctgta tccaattatc ttccacaagg agtcgatttt aaagtccaat
     6241 tgccgttgaa gcctaaaaaa taatgcttat ccctaaaaaa gggctgtgac aaaaatcttg
     6301 tcacagccct tttttgttgc gaataaacgg tgttccagaa gtagcttctt cggaaataag
     6361 ccgaaatttc tttaagctga ccacttctgt cacaacctct ttagcttacg cttacgattt
     6421 tttattgcgg tcttcaatgt gtacatcacc gcttgtgtca atatccaatt cttctcggct
     6481 aacatcttct gtcacttttt caacatcttc atgttcttct ttatgaatcc caacttcttc
     6541 acgaataact gtatgtttgc ttacatctac ttgctcttct ttgattggga tagtaatcgt
     6601 gtcatcttcc aatttttcgt ctgttccctg tgaagattga tctgttactg gtttgcgttc
     6661 gatgacgatt tcttcacgtt tgaccggtac ttctaccgtt tcagtatcgt tgaccacatg
     6721 tttgtggatg tcgacttctc cagttgtcac atcatgtgta tttacatcta atttttcttc
     6781 ttttagttga attgtatcat catctagatt tggtttgcga tcgatgtctt cagtcttgct
     6841 tgtacccatt gtatcagtct cgccaggtac gtccatcgga tcagttcctg gtgtcatatt
     6901 caatggatcc gttgtatttt gaaagcctgt cgtgccacct acgtccattg gatctgttcc
     6961 agtgccagtt tctaatccag tccccattgt gcttgctggg ctaacagtat cttgtgtatc
     7021 cattgtatct tctgttgttt ctggctgacg atagcctttt actgtgatga caaattttcc
     7081 atcagaaata tctcctctat attgatataa aggatcattt tctggtgtag ccgtctcaga
     7141 atcataggta tcaaaagaaa aagtatcttt tattttttcc cataaagaac ggtcttcttc
     7201 accatccatc cgattttcat ctgtttcaac atctatacca gacaatccgt caaatcctag
     7261 tctagatgct gcttcatcag tagtataaat aacgatatca tctcgttcat acccttcgtt
     7321 acgcaaccgt tcaattactg ataatgcttc ttccctcgta ctatagcttc cgatgaccgt
     7381 taaatttttc tcttccatgt aaattcctcc tagcaaatag atttatgatt ggtatattgt
     7441 tacttataaa tttattctat aggagacgat tg
//
LOCUS       DAYYCY010000122         7368 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000122 DAYYCY010000000
VERSION     DAYYCY010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.122"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            3..803
                     /locus_tag="ACHAZB_002120"
     CDS             3..803
                     /locus_tag="ACHAZB_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HFM8614756.1"
                     /translation="MYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYDCTVILGMKDV
                     RTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTKKITIDDAYEQ
                     MLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNGISVMMYLSVN
                     GNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFVKEVKEAFAKA
                     FEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL"
     gene            787..2154
                     /locus_tag="ACHAZB_002121"
     CDS             787..2154
                     /locus_tag="ACHAZB_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFM8614757.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            2173..2982
                     /gene="yidA"
                     /locus_tag="ACHAZB_002122"
     CDS             2173..2982
                     /gene="yidA"
                     /locus_tag="ACHAZB_002122"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFM8614758.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(3025..3468)
                     /locus_tag="ACHAZB_002123"
     CDS             complement(3025..3468)
                     /locus_tag="ACHAZB_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFM8614759.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            3602..3973
                     /locus_tag="ACHAZB_002124"
     CDS             3602..3973
                     /locus_tag="ACHAZB_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8614760.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            complement(4142..4924)
                     /locus_tag="ACHAZB_002125"
     CDS             complement(4142..4924)
                     /locus_tag="ACHAZB_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HFM8614761.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(4921..5892)
                     /locus_tag="ACHAZB_002126"
     CDS             complement(4921..5892)
                     /locus_tag="ACHAZB_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HFM8614762.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            5984..>7368
                     /locus_tag="ACHAZB_002127"
     CDS             5984..>7368
                     /locus_tag="ACHAZB_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFM8614763.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKK"
BASE COUNT         2340 a         1463 c         1285 g         2280 t
ORIGIN      
        1 acatttatca aaaaaacgac ttttcccctt ttgcattaac agatatatta acggaatatt
       61 ctaaagaaca ccagcaaatc tttttacatt tctggcaata tgactgcaca gtcatcttag
      121 gtatgaagga tgttcgaact ccttttctaa gtgacgggat ccaaacactg aaagatgcag
      181 gttacgctcc agtgatcaga aattcaggtg gattagggat cgtatcagat gaaggaattt
      241 tgaatatttc tttgattttc ccccaaagtg acacaaaaaa aataaccata gatgacgctt
      301 acgagcaaat gctcctattg actaaatacg cttttccaaa ggcaccgata caagcattcg
      361 aaatcaccca ttcttattgt cctggaactt atgatttaag catccatggc aaaaaatttg
      421 ccggtatcgc tcagcgtcgg atcaaaaatg ggatttctgt tatgatgtac ttaagtgtca
      481 acggtaatca ggaaaaacgc gggcaagtca tgaagcgatt ttatcaatct gctttaaaag
      541 aagactttgg tacaaatggc tatcctgcag tggatcctgc tacaatggca acacttgaag
      601 aactgcttga ccaacccctt tttgtaaaag aagtcaaaga agcatttgca aaagcttttg
      661 aaaagctgta ttctgcaagt gatttacagg attctaccga ctggctaaaa aaagaaatcc
      721 agctggagca atggaacaac caaatccaga gaatggaaca acgtaataac ataaaggagc
      781 tacattatga caacccctta taaatatcaa atgctgccta tggaaaaagt ttttcgtgat
      841 ccagtccata actatatcca tgtccagcat caagtaatct tagatttgat caattcgaaa
      901 gaagtccaac gtctacgacg cattaaacaa ttaggcactt cttctttcac ttttcacgga
      961 gcagaacata gccgttttac tcattcatta ggcgtttatg agatttcaag acgaatctgc
     1021 gatatctttt ctcgaaattt ttcaaaagaa aaaataggca acggaggctg ggatgatcag
     1081 gaacggctgg tgactttatg tgcggcactg ttgcatgatg tcggtcatgg tccttactct
     1141 catacatttg aacatatctt ccatacgaat catgaagcca tcactgtaga gatcatcact
     1201 tctccagaaa cagaagtgta tcagattctt aaccgagtag aagaaggttt tcctgaaaag
     1261 gtagctagtg tgatccaaaa aacttacccc aacccacaag ttgtccaaat gatttctagt
     1321 cagatcgatg cagatcgaat ggactatttg cttcgggatg cttatttcac tggaacagaa
     1381 tatggaacat ttgatcttac ccgaatatta cgtgtgattc gtccttatga aggcgggatc
     1441 gctttttcca tgagtggcat gcatgcagtg gaagattata tcgttagccg atatcaaatg
     1501 tatgtgcaag tgtacttcca tcctgtttcc cgtggtatgg aagtaatttt ggagcatctg
     1561 cttcatcgcg cacacgagtt gtatcaagaa gatgccagca gttttgaact ccattctcaa
     1621 ttacttctgc catttcttga cggaagcttt actttagaag aatacctcaa acttgatgat
     1681 ggggtcttgg gcacttactt tactcaatgg tctgaatctg ctgatcctat tttaaatgat
     1741 ttagctaaac gttttttgaa tcgtaaacca ctgaaatctg ctactttttc tggcaaccgg
     1801 gattcaaaat tagtccaaga actcactttg ttagtagaaa aggtcggata taatcctgtt
     1861 tattacactg ctgtgaattc tagctacgat ctaccttatg atttttatcg cccagaacaa
     1921 ggcagacatc gcacacaaat cgagattttg cgaaatgacg gaactttgat agaattatca
     1981 caggttagtc agcttgttgc tgcattagcg ggacaagaac aaggagatga gcgatttttc
     2041 tttccaaaag aaatggttga tccgtccttg cgtgatcatt atgatttatt tgacgaaact
     2101 tatcaagaat ttgccagcca tatccgtaat ggcgcattga ttgaaataaa ttaaaatata
     2161 ttggaggaaa ttatgtctat caaactagtc gctatcgaca tcgatggtac tttattaaat
     2221 gagaaaagag aagtaacaaa agaagtaaaa gaagcgattg ccgctgctgt tgcaaaaggt
     2281 gtttcgatcg ttttatgcac tggccgtccc ttaccaggtg tccaggaaca gttaaacgag
     2341 ttgaaccttt ttcaagataa tgattacgtt attacttata acggggcact cgtacaacaa
     2401 acgaaatcag gtaaaatcat cgcccgtcac ggactgacac atgaagattt cctagagatc
     2461 gaagtgatgg cacgccgtgt cggcagtcat ctccattcga tcgatgacaa agcgatctac
     2521 acgcctaatc gagatatcag cgcttatacg atccacgaag caagtttagt caagatgccg
     2581 ctaaaatatc gaacacctga agaaatgaca ccagacatga acatcgtcaa aatgatgatg
     2641 atcgacgaac cagaaatttt agatgcagca attgcgcgtt taccgcaaac tttccgtgat
     2701 aaatacacaa cggtcaaaag tgcgccctat tacttcgaag tactaaataa agaagcaagt
     2761 aaaggtgcgg cagttgcgaa tctcgcacag caccttggta ttgatcaaga tgaaatcatg
     2821 gctatcggag ataacgagaa cgacttatcc atgattgaat atgctggtct aggagtagca
     2881 atgggaaatg ccgtttctct tgtcaaagaa gcagcaaatg tcatcacttc atcaaacgac
     2941 gagcacggtg tagctgaagc aatcaaaaaa tatgtactgt aataaataaa aaaactgaga
     3001 gaaagctgat ctactcgctt tctttcagtt tttatttttt tcgatccatc tctcgactgc
     3061 ccatcggtgg ctaccacgtt tcaatttcac aagcgcttct tgagggctca cccaccaaat
     3121 tttattggtc ttttcagttg gttctcctat acgcttccaa gaatcagcaa caaagaaata
     3181 tcctggatta taataataag ttgcacgata attcgagtaa aaatactcat cagcttctcc
     3241 cagataagaa gaaatttctg ccacaattcc catttcttct atcagctcac ggacaatcgc
     3301 ttcttctttc gtctcttctc cttcaatttc cccgccagga agaaaaaaag caccatttgg
     3361 agcctcgatt actgcaatct gctgatctct ttctatgaca atatatgctg cataacgggt
     3421 ttgataatca gcaaattgct ttttttcacc aaaaattggt ttatccatga atagtttcca
     3481 ccttaacagt tttattttct atccatttta catgagaaat ttctcatgaa caagcacaaa
     3541 tcaactattt ccttacttct ttttgttatg attgagtaaa aggaaaagga gagatgaatc
     3601 tatgaaaatg gctcacacat gtgttcgagt gaaagatctt gaagcatcaa tcgaatttta
     3661 tcaaaaggca tttggttttg aagaaagccg ccgccgtgat tttccagaaa acaaatttac
     3721 acttgtttat ttgacacttc ctggggatga ctatgaatta gagttgactt acaactatga
     3781 tcatggtgcg tatgatctag gagacggcta tggtcatatc gctatagcag ctgatgatct
     3841 agaaaaatta cacgagcaac ataaagctgc cggtcttgag attactgact taaaaggatt
     3901 acctggcact gcgccttctt actactttgt cattgaccct gatggttata aaatcgaagt
     3961 catccgagga tagttagtat ggggcatttt actcttgatt gaataaaaaa caaacaccaa
     4021 taattaaaat gagacgcaaa cttttcatta aattgaaaaa gtttgcgtct ttttttaaca
     4081 accattgtag ctataaggtt gccactctcc accaatttat ttttaattca tttgaaagaa
     4141 atcaaataaa cgaggtacct ttagaaaaca aaactgcttt tcctcctatt atcacacgat
     4201 cttcttttaa ttcacattct agatccccgc cccttgatga catttgataa gctgacaatt
     4261 ttgttttatt cattctttct gcccaataag gaataagatt agaatgtgct gatccacata
     4321 ctggatcttc attgattgtt aattttggaa agaaggttct agaaacaaag tctttttgca
     4381 cactttctgc tgtcacgatg actcctattc ctaattcaaa atttttgata gctgtaaaat
     4441 ctggctgcaa tttagccacc gttttttcat cttctaatac gaaaaataaa tcacgtgcta
     4501 agtaggcttc tttaatctta gccccaatcg cttcttcata ttcagacaat atcggcactt
     4561 ttttcggcat aatactagga aaatccatgt aataataatc tttaattctt gtcacaaata
     4621 gattaccgct ttgagtagaa aatttaatcg tttcatcagg tattttgtaa tagttaaata
     4681 aaacaaatgc tgttgctaat gtcgcgtgac cacaaagatc gatttctcga tctggcgtaa
     4741 accatctaag ctcaaattct tggtttttct tcactgtaaa tgctgtttca gataaattgt
     4801 tttcaatggc aattttttgc atcgtacctt ctggcagcca ttcttctagc acataaaccg
     4861 ctgctggatt tcctttgaac acctcatctg caaatgcatc aactacgtaa taatctaagt
     4921 tcattttaaa gcccctttct ctaatttgtt ataaatatat tctgcacaac aaagatcttc
     4981 aatagctaga ccaactgcgt cgaaaatcgt cggcctcgat tctcctttat aaaatgtttt
     5041 tttctcagaa actaattcgc ctaatgtacc aacaatacaa gattcctcta aatctccttc
     5101 tgccatggct ataagcaggt ccccgctttc tgttaaggca gctgagtaat catctacata
     5161 gatttgagaa gtttccatca acgatgtact tatctctctt gtatcaggag taaatgtgcc
     5221 cacagcattg atatgtgcgt cttcccttac ccactctttt tgaagaaatg cctctttact
     5281 agaggtcaac gtacaaatga tttccgctgg acgtactgca tcttctactg atattgcatt
     5341 tataaacgtt attttaggat actgtttttt agctttagcg ataaaagtat ttcgtctttc
     5401 ttctaaaata tcgtaaacaa acactgtagt gatgggacga atggtagaaa tcgcagtcaa
     5461 atgcgacaat gcttgttgac cagctccaat taaagcaagt gtttctgcat gcggatttgc
     5521 taagtaatct gttgccagcg cagatacagc agctgttcga atccaggtga ctgcatttgc
     5581 gtttatcaat gctttgggta gtccgttttg agcatcaaac aagaaaatct ctcctaaatg
     5641 agaaggtaaa tgatactgat gattatccgg aaaagcggta atgacttttg tgccaaaata
     5701 tctgttctcc cccaaataag caggcatcat cgcaaacata tttttgcctt ttccgtcagg
     5761 taaaatttcc accaatcgag gattttggct gattttacca ctttcataat caaaaaaaca
     5821 ttttttcatt acttgaatcg cttcttcaaa actaaaagaa gctactactt cctcaaaatt
     5881 aataattttc atagacaatt ccctcctcac aaaagattaa cttctaaaat caatacaatc
     5941 aatggtaaaa ttgtatggta tacaatttta aggagtggag aaaatgccta ttaattctta
     6001 tgacaattac ccgttaactt ggcgaccaga caaacaaaaa ttgaagcgtc cttattatcg
     6061 atcgttgatt caacaattag aacaagatat tttaaagaat aaattacaga aaaacactca
     6121 gttaccctcc caaagagagc tagctgatta tttagattta aattttacta ccgttggcaa
     6181 ggcttataaa tatggaattg agaaaggttt gctgtatacg aatattggaa gtggcacatt
     6241 tatttctccc aatgtctttg agtccatcac catctctacc aatgaagtaa ccgaacatat
     6301 cattgatttt ggtctggtta gttcttttga agaatgtaat aaattcgttg tcccgtttat
     6361 tcaatcaact agtaataacc caaatcttgt tggtttgctt aattaccaag atcccaaagg
     6421 cacaaatttt caattgacta ctgcagcaaa ttggttaaaa actcaaggag tcattccgtc
     6481 cttagaatct ctatctattg tttccggtgt tcaaaatgga ttggctatca cactttctgc
     6541 acttttctct ccaggtgata gaatagcaac agaccgatat acatacgcta atttcattga
     6601 attggctcag ttatatcatt tagagatcgt tccaattgat tttgacgagc aaggaatgcg
     6661 acctgatata ttgattcagg aatgtagaaa aaagaagatt catggtgtct ttcttatgcc
     6721 ttcttgtaat aatcctatag ggtttcaaat ctctcacaaa agacgagagc aattagccga
     6781 agtgattgaa aaagaaggtc tatgggttat tgaagatgat atccattcat ttttaacaac
     6841 ttactatcaa catacgatca ttcctccttt ccaaaattta ttacctaacc aaacaatcta
     6901 cttagctgga atgacaaaat ttatttgttc tggattgcga gttgcttatc ttgtagttcc
     6961 agtacatgtc caaagcaaga tagaacaagc aatttttaat ataaatgtga aaacttctgg
     7021 attagatgtg gaagtcatca ctcaaatcct ccattcaaat gaagcaaata ggatcttaga
     7081 aactaaatta agtcttacaa aaagagccaa tcagcttttt gattctcttt tcaaagtaga
     7141 aaaaccaacg aatcgtgtcc cttactatcg aattcttcca gttgatcctg agattgaccc
     7201 taagataatt gagaatgact ttcttgctaa cggtatccga ctttatcatt ccagacgctt
     7261 tactacacgt gcgcaaccag atgcattcgt tcgaatttcc ctatcctcta ataaccttga
     7321 aattttagct tctggactaa gtatcgtaaa agaaaaaata aaaaaata
//
LOCUS       DAYYCY010000123         7356 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000123 DAYYCY010000000
VERSION     DAYYCY010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7356
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.123"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            131..3658
                     /locus_tag="ACHAZB_002128"
     CDS             131..3658
                     /locus_tag="ACHAZB_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFM8614764.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3655..>7356
                     /gene="addA"
                     /locus_tag="ACHAZB_002129"
     CDS             3655..>7356
                     /gene="addA"
                     /locus_tag="ACHAZB_002129"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HFM8614765.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQ"
BASE COUNT         2532 a         1270 c         1544 g         2010 t
ORIGIN      
        1 tttgcgtata cttaaaaaag tagaaatatc agaaccttaa aaggatataa tttgaagaat
       61 atagaacaaa tgttcagtat tttttgatat aataaaggag caccgtatca tgtgttgaag
      121 gaggaaaagc atgtctttac aatttattct tggaaacggg aaaaaagatc accgtcaagt
      181 cttgatcgac gaagcatccg cttggctcga ccaggatgca agtaaccaag tatttttcct
      241 tgttcccaac tacagcaaat tcgaacagga acaagaaatt ttagcggaga tgcgaagaag
      301 aagcggaaaa aaaactttca gcacaacaaa cgttcaagta tttagttttt atcgtctggc
      361 ttggtatttg ctgcaacaga caactcttct tacaggaaat gaattatcag aatcaggatc
      421 tgcaatgatc ttgcgacaga ttttagaaaa aaatgaggaa gaattaacaa tctttcgcgg
      481 agaaatcaat aaaagagggt ttattcgtca attacaagaa ctctacagtg aattgcgtac
      541 aggaaatatt caaccagaag acctgatgct tttatttggt gattcaccta atgcagaaga
      601 ccagcaactt aaaatgaaag acttgaagct gattttttca gcttatgaca gcgaattgac
      661 tcaacgtgca ctaacaaatg aagatgcact atctatacta gcaaattata tgcagacaca
      721 agatttcaac aacgtcaaat ttattgtcag tggtttttct cgaatcaatg gtcaagagta
      781 tcaattactg caggcaatga tggaaaaggg gcaattggtt gtcgatttat tgctggatcg
      841 tccttataca gcggatcttc cagaagtact tacgctcttt catgagactg gaaagcttta
      901 tttccgtctt tttcaaagtg caagagaacg gaaagtccct atattagtgg accgatttgc
      961 accagagcag actcgtccga cagagcttca actgctgcaa acaatgtggg aagagacatc
     1021 tagccaaaag aaagcggcag tacacaaaat agggggacaa gagcatctcc atttgtggga
     1081 agctgaaaat ccaactgaag aagtccgaca attagctgtt gaaatcagac gactagtcag
     1141 tgaagaaaac taccgttatc gtgatatcca agtgctgaca aaaaatatgt ctttatatgg
     1201 aaatatcctt cggccgatct ttcaagaaat ggcaattcct ttttatatcg atgaagaaca
     1261 aatgatggaa aatcatccgc tgatcgaatg gatcaacagt ctgttcgctc ttgaccgcta
     1321 ttcttatcgg ctaagtgata ttatgcgttt tttcaaaact gaattatttc tgtcgacgga
     1381 agcagaaacc gaacttgatg aatggaaaca agagcgagat gaattcagga aaaaactgga
     1441 catcactgaa aatgtagcac ttgcttacaa ttatcaagga acttattgga ctcgtgaaaa
     1501 agactggcag tttgtggcgt atgattttga agcagaacag attgaagacg tacagtattt
     1561 ggaagaacag tcaaatacga ttagacgatt atttcagaaa gctgtaccag tatttttgaa
     1621 aaaaataaag aaaagcaaaa caggacaaga ggccgcaaat gttttttatc aatttttgat
     1681 ccaaagtggc gtacagcaac aattattgtt ttggcgtaat caggaagtag acagaggaaa
     1741 cttagaagcg gcaagaaacc atgagcagac atggggagcg ctgattgatc tgttggatga
     1801 atatgtagta atttatggtg aatcggcttt tgactggtca ctgttccaag aaatcattac
     1861 aagtggtcta gaaaatctaa gttatggtaa aatccctacc gcaatcgacc aagtgcgaat
     1921 caatcgtttg gaattagttc gaccaaatca agctgccgtc acttttgcat tagggttgaa
     1981 tgatcaagtg tttccagacc gaaaagagac aaaaggattg ttgtccagtg aagaacgaga
     2041 agtattaaat caggccttgc cagatggaca gtttttattc gacccatcaa aggaaagtat
     2101 ctcttttgag cctttccaag cctatcttgt ttttctttcc ggaacagatc gattatatct
     2161 tagttatgca caaagctatg acacagatgg ttcgctgaag atgtctcctt atttgcgtag
     2221 aatcacagaa tatttatctg ttcccgtcga gagaaaagag catttgacga tcgaaagcga
     2281 ccctaattgt tatgtaggga cttacagaag cggaatcaat acagtcaata ggatttatcg
     2341 attagcttta gatgaaaaaa aacctgtacc aaagcactgg caaaagctca aagaattagt
     2401 actgaattcg tcatggcgaa attttgcttt acagattttt gaaagtcagg aacatttaaa
     2461 tgttcctgtt tcgatgccag aaaaaatggc ggaagagctt tatggaaaag agatttatac
     2521 ttcaatttca cgtatggaga atttttataa ttgtgaatac aaatacttca tgcaatttgg
     2581 tttgaaattg aaagaacgaa ctgtttatgg tctaactcca gcagcaactg gtgattttta
     2641 tcatgaatca ttggatcgtt tcttcaagct actgttttca aaccaattat ccttagtgaa
     2701 tatgtccgat gcgcagcgta gagactttac tgaaaaagtg ctgcaggaag tattcggaga
     2761 acttcggttt gagatattgg atagttctgc aagaatgaac tatatccgct accagttagg
     2821 acagacgatt caaaaagtga catgggcttt gcaaaaacag agcaaaaata caggcatgca
     2881 tcctcttcag acagagattc tatttggtca gattgctgga gcaaaaggta tcacaggtct
     2941 ggaattaccg ttgagtaatg gtggaaaact ccatgttcga gggaaaatcg atcgaatcga
     3001 tgtagcttct gaacaagaag atacttggct aagcgtcgtt gattacaaat ctagcggacg
     3061 ttcattcgat gtcacagaag cttattatgg catggctatg caattattga cgtatctgga
     3121 cgtagcattg atggatgcgg tacgtttgac tggaaaggcg gctgttaaac cagcaggatc
     3181 atattattta catgttcata atcctgtatt gacggagaca gatgatgtag agaagaatac
     3241 attgaaaaaa tttagttatg atggcgtttt tgtcgatgat cccgctcttt tagaagtact
     3301 agatcattca ctggaagcaa aagaacaatc actgatctat ccagtgaaaa aaaatgcgaa
     3361 agatgaatac caaaaaggta gtttcagtaa agacaaattt ttcacggaag aagaactgca
     3421 actattcatg gcgcataatc gggaaaatat gcggttagct ggagatgcga ttacttccgg
     3481 agaaatcaaa ctaaatccag cttataaaga taaacagcgg attgcctgcg aattttgtcc
     3541 attcagaagc atctgtacgt ttgatgtgat gctgaaagaa aataattacc accgtctgga
     3601 gaaaatagat aaggaagaag cactgcgtag aatattgaaa aggagtgaag aagagtgaat
     3661 caaattcctt taaaaccgca aaatgaacga ttcacagatg atcagtggca agcgattttt
     3721 gatcaaggag ataatctatt agtctctgcg tctgccggat caggtaaaac tacagtgctt
     3781 gttcgaagag tgattgaaaa actaaaaatg ggttttgata tcgatgaatt attgattgtg
     3841 acatttactg aagctgccgc aagagaaatg aaagaaagaa ttcaagaggc attacaagaa
     3901 tccgtcaata gcgaaagtga ccctgtacgt agacagcatt ttactaagca gttagtttta
     3961 ttgcccacag cgaatatctc aaccttacat gctttttgcc tgacagttat tcgacgttat
     4021 tattacttaa ttgatattga tccagtcttt cgcatgttaa cagatgaaac agaaacgatc
     4081 ttgatgaagg aggatgtgtg ggacgagctg agagaagcac tttatgcaga aaatgatgaa
     4141 cgatttttcc aattaacaat gaatttttct aatgatcgtt cagatgacgg cttgacgaat
     4201 ctggtgtttt ctctttatga gtttgcccgt gccaaccctg acccccaaaa atggctagaa
     4261 cagttaagcg ataattatcg tttgcctgag gggctagcga aatctcgatt atatcaaaaa
     4321 caaatccgtc ctttagtttt ggcagatata taccaatgtg tacagttata tgaacagatg
     4381 actcagttag cacaaggtga aggattagaa aaaatgcatg agcaagtagc tggagaacaa
     4441 cagcagatca agaatatcta tgaagcgttc tcgcaagatc ggttagaaga agcttatgct
     4501 ggtttggaac aactaacttt ttcaactttt aagagcagtc ggaaagcaga attgaaagaa
     4561 atttccaatg aagtcaaggg catgagggat aaagcaaaaa aactgattca gcagataagt
     4621 aaaagttatt ttcctgtttc cccaagccaa atggaagaat taacggacaa agcactaccg
     4681 ttagtcgaag aaatgacaaa agtcacgcaa agcttcatgg acggtttcag tatgcgtaaa
     4741 cgggaaaaag gtgtgctgga tttcaatgat ctggaacatt tagcgttgca gattctcaca
     4801 gaaaaaacga aagatgtatg gctgccaagt gaagcttcaa aacattatcg aaaaaaattc
     4861 aaagaagtaa tggtagatga atatcaagac gtgaaccaat tacaagaagc cattttatac
     4921 tggctaagag aacccgatga tacaaaagga aatatgttca tggttgggga tgtcaaacaa
     4981 tccatctatt ctttccgttt agcagatcct agtttattta tcgggaaata cgagaatttc
     5041 tcaaagaaag aaggcggacg tcgaatcgtt ttggctgaga acttccgctc tagaaaagaa
     5101 gtcctttctt ttacgaatct tatttttgaa caactaatgg atccagcagt gggtcaaatc
     5161 aattatgacg aagcagccaa actgatccaa ggattttcag actttccaga gaatgaacag
     5221 tttgagccgg aaatcatgat ctatgaaaaa gaacaggaag aatcagagat tgagattccg
     5281 acggatgata tactggaaga caaaacagaa ggcgaacttt ttatgacagg actaaaaatt
     5341 cgtcaattaa ttgattcctc ctttatgatt tatgataaaa aatcaaaaaa aagtcgtccg
     5401 atagagtaca aagacattgt tctcttgaca cctacaaaga agaacaactt aacgatttta
     5461 gagattttca aaacattgga tattccatta gagatgaatg atgctcaaaa ctattttcaa
     5521 gcgacagaaa ttcgcacgat gatctcgctg ctacaattaa tcgataatcc ctatcaagat
     5581 attccgcttg cggctgtatt gcgctcaccg atcgtgggtc tgattgaacc agaattagcc
     5641 agcattcgat tagctaaccg tgcacatact tactatgatg ctgtgcttgc ttatcaagca
     5701 tcaaacgaag atgaattagc agctaagctg gaacattttg gcaagcaact agagcattgg
     5761 cgagaacttg ctcgccgttc ttcgatcact gacttgttat gggatatcta ttatgagacg
     5821 ggatatctag agtatgtggt cggtctgcca gcaggggcac agagacaagc caatctttat
     5881 gcattggttg atcgtgccaa agcttacgag caaagttctt tcagaggatt ataccagttc
     5941 gtgcgtttca ttgaaaaaat gcaagaaaaa gataaagact tagcagaacc agtcatttca
     6001 atagaagata atgctgttcg cgttatgacg atccatgcaa gtaaaggctt ggaattccca
     6061 gttgtatttt tgttagatat gacaaaggag ttcaacttac aagacttgcg caatcggtat
     6121 gcttttgaag aaaaacttgg agcaggaatt cgctatatgg atcctgaaac acgagtgtta
     6181 tatgatacgt taccgtttca agccatcaaa ttggcaaagc aaaacaaatt gttgtcggaa
     6241 gaaatgcgta aattgtatgt aggattgact cgggcagaac aaaagctttt cattgtcggt
     6301 tcctataaaa ataaagagca aatgatccaa acgtggtctg aagcagctga tcatgaggag
     6361 ttagtttttg atcctgcgct acgtttgaag ggcagaagca gcttgatgaa ttggattggt
     6421 tacggattga tccgtcatcc tgaaatgcag aaatacttag aggaagaaat ttcgacttct
     6481 cttcttcaac attcaaatgc acagttttct atttcatgga tgaatcagca atcgatcatt
     6541 gaacaaagac agttgcttgc tgaaaaagaa ctggttaact tagatcagca gatgaaagaa
     6601 gatgaaactt tattagcaga ttcgttacaa aaacgtctgg catatgaata tccttatcaa
     6661 gcttcttcac aaaccacaag ttatcaatct gtatctgaaa tcaaacgatt attcgaagat
     6721 ccagatgata cacaagaatc acgtttgact ctggaaagca gtcagaacaa agcagcatcc
     6781 cgtcaatttc gttatacgca agaacaacta gcagaaccaa aatttttgca aaaagatcgg
     6841 caagtgtcag cagctgctgt tggaactgct acgcatgctt tgttacaatt actgccatta
     6901 gagatgccaa ctacagagag tatccatcaa aaattacagg aattggtaag gaaacggtta
     6961 gtagacgaaa aagtcgcgaa aaaagtagat gtttcttcta ttatatggtt ctttcaaaca
     7021 gaacttggtc aacagctgat tgccaacaaa gaaaacgtaa aacgagaaca acctttttcc
     7081 atgctgcttc ctgctgacga agtatttcaa gactatccaa atcaagaaga tgaactatta
     7141 atccacggaa tcgtcgatgg atacctagaa gaaaaagatc atctcaatat ctatgatttt
     7201 aaaacagatt ttattttaca tccggatgat ccagcagaaa tagatgcaat cgtccaaaaa
     7261 tatcaaggac agctaagact ttaccaacaa gcaatgtcag aagctttgaa taagccagta
     7321 gaaaatgttt tcctaatttt gcttcgagtg aaacaa
//
LOCUS       DAYYCY010000124         7345 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000124 DAYYCY010000000
VERSION     DAYYCY010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7345
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.124"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(146..1111)
                     /locus_tag="ACHAZB_002130"
     CDS             complement(146..1111)
                     /locus_tag="ACHAZB_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HFM8614766.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            1345..2190
                     /locus_tag="ACHAZB_002131"
     CDS             1345..2190
                     /locus_tag="ACHAZB_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM8614767.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            2381..3568
                     /locus_tag="ACHAZB_002132"
     CDS             2381..3568
                     /locus_tag="ACHAZB_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFM8614768.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            3574..3930
                     /locus_tag="ACHAZB_002133"
     CDS             3574..3930
                     /locus_tag="ACHAZB_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HFM8614769.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            3932..4270
                     /locus_tag="ACHAZB_002134"
     CDS             3932..4270
                     /locus_tag="ACHAZB_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HFM8614770.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     misc_binding    4346..4602
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            4665..5699
                     /locus_tag="ACHAZB_002135"
     CDS             4665..5699
                     /locus_tag="ACHAZB_002135"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFM8614771.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            5692..6378
                     /locus_tag="ACHAZB_002136"
     CDS             5692..6378
                     /locus_tag="ACHAZB_002136"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFM8614772.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            6402..7250
                     /locus_tag="ACHAZB_002137"
     CDS             6402..7250
                     /locus_tag="ACHAZB_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFM8614773.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
BASE COUNT         2433 a         1309 c         1599 g         2004 t
ORIGIN      
        1 ttacgacctc ttggtaaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaaccag
       61 tacaaaaaca tgaacagctg tttttgtttg gccgttctta ttacttgatg cagaacagct
      121 ttttcacaac ctattgaatc gattattaag cgattttttt agaaacaaat tttccatgtt
      181 tgctatgttc tttaattaag aaacgtacgt aatgagcaag aacgtagaag acaactacac
      241 ttgcaaacag acattccgca acttttaaga aatccaatac gctactatag atacctaaac
      301 tgttgaagta atgaacagta gtaaatatag ctgttgcaga gataacaagc gggaatgtaa
      361 aggctgcata gcttggataa aaacgaagct tgaacaaacg tggaagcata gacagcacaa
      421 cgaaatacaa tacttgagat aaagcgaaca atgctacgac aagataaaaa ttaggttcag
      481 caaagttttt aagataacct gttaggcaaa gagaaccagg tgctgcaacg atcgtaatca
      541 gcggacgtgt cggatgagcc atatttctta cgaaaaagac acgtttgatg atgatcggca
      601 ataagaacgc atagcagatc aatgaaatcc aaaagaaaat ttttccaata agtgggaaga
      661 agttcccggc agttacagtg atgaccccaa atccaacgta aatgataaac cagcttggat
      721 aaattgcttc aattgaaaga tcagctttga taacatggtg ataattgaaa taaaagacaa
      781 ggatcacttg gaagataaca gcgcacagcc aaatgaattt gacaattggt gcaatacttt
      841 catatgaaac aaaataagta caaataacca tgagtgacat cgtaaaagtt ggtgaaacgg
      901 aggcgatgat tggatcatgc aacgtttgtt tcgtgtgttc gaataagata aacaatttaa
      961 caaaaatgaa tatcataaga atcatagaga taccgccagt tagattgcct aaagcaacat
     1021 ggtgatagtc tttaaacaga tttccaaggg aagctaaccc aaggatcaac ccgcagatcg
     1081 ggatcggaat aattttcaaa aagttcttca tatataatat tctccttttt atttaataga
     1141 ttttagtacc aagatgaacg aatacattgt aaaatgaata ggaatgtatg attacgagca
     1201 agcagacagc tgattgtctt catctaagtt actagttgga tcagcaactg ttttgttgta
     1261 catttctatt atagatggtt ttttgtatat ttaaatcaaa tagtttttat agtaattatc
     1321 ataaaaagtt atggaagtgg tgccatgttt aagttattaa cgacttttcg agcagtatat
     1381 gaaacaagaa atttttccag agcagcagaa gtactgtttt tatctcaacc agcagtctcg
     1441 aatcaaatca aacagttgga gcgggaattg aatatcgatc tttttcaacg taacggaaga
     1501 aaagagatgc ttccaactaa gcaagccgac ttattatatg aacggacact ggttttgtta
     1561 gaagaatggg aagaaactca gcaaaaattg ctcaatgaac aaaatgaaga agaagtatgt
     1621 cggatcgttg cttcccacac ctttgctgtc tatcttttgc ctcagttgat gaaacatctg
     1681 atcaaaaggt ttcctaaagt gcattttgag atcgaaatca cgaattctca tgaatcatta
     1741 gaacaagtag cgaaacatga atccgatttt ggttttatcg aaaagccatt agagacttcc
     1801 ggcgtacaaa gatactcttt gatggaagac cagctagtca tcgctgggga cccaaaaagc
     1861 aaattgtggc ttgtaagaga agcaaactct ggtgtttttc attacacgca gcgttatttt
     1921 gaagagatga atatccaaga taagaagatg atcgtgaaaa gtaacgatgt aattgttgcg
     1981 ttgctgaagg aaggaatcgg tcgctcgatt ttgtctaagc gctctgttcc agaaaatgtg
     2041 ccaatgactc cgttgaattt ccatcgatat ttttatttca tccaacgaac caatctggtt
     2101 tctgatcagc tgctagagat tgccgaagag atccttcgat acaatgaaag cacagaaact
     2161 tctcacagcg aggagaatct cagctggtag ataattgcat gtatcaaaaa aagttctatg
     2221 atacactaaa gataagcagt aggcttttgt actgcatttg ctcaagcaac tgcacaaacg
     2281 gaaagttaat ttttcccaaa tgtctatgga aagactttcc attatctaac tggaccgcta
     2341 taataatcag cagtatggtt tttaagaaaa gggatgaaac atggaaaaaa aattaaaagc
     2401 ttcgaacgat aatcgtattg attatggcgt gattttacca gtctttctgt tatgtttgat
     2461 tggtctgctc tcattatatg tagcacttag ccatgatccg aacaatccag cagtcttgaa
     2521 aggcgtaggg atgcaagttg tctggtattt agttggagcg gcagcaatcg ttgtcatcat
     2581 gcatttgaat tccaaatgga tctggaaact gactccttac ctttatggtt tggggttagt
     2641 ggtaatggga ctcttgctca aattctatga tcagaattta gctgcttcaa cagggtctaa
     2701 aaactggttt cgattcggtg cgtttacttt ccagccagca gagttgatga agattgcgta
     2761 catcttgatg atggcactga tcgtaactaa acataatacg caagtaaaag aacggacgat
     2821 gaaatcggac ttttggctga ttgggaagct cttgatcgtg acaataccgg ttttagcatt
     2881 gatcatggcc caagatgact ttggaacgat gcttgtcttt cttgcgatct ttggcgggat
     2941 tttcctgatg tctggaatct cttggcgcat catcgcacct gtagtaatct tggcagtagt
     3001 cgttggagca ggaacgatct tcttggttac gactgaaggc ggaagagact tgctttataa
     3061 agttggattc aaatcttatc agtttgcacg tattgattca tggctggacc ctttccatga
     3121 tacgtcagga atgagttatc agccggccca aggacttttg gcaattggta ccggtggctt
     3181 gtttggtaaa ggattcaacg taagcaatat ctatgtgcct gttcgtgagt ccgatatgat
     3241 ttttaccgtt atcggtgaaa acttcggctt tatcggtggt gcattcgtca ttttcttgta
     3301 ttttatttta atctatcgta tgattcgggt gtgtttcgat acgaataatg aattttacgc
     3361 ctatatcgca agcggtttga tcatgatgct gctgttccat gtctttgaga atattggagc
     3421 aaatatcgga ttacttcctt taacagggat cccattgcct ttcattagtc agggaggatc
     3481 ctcgatttta gggaacatga tcggtatcgg gttgatatta tcgatgagat accaaaatga
     3541 agcacctgca agatcttcaa ggaggtaaga aaattgtaca ccttttactg gtacccgaaa
     3601 tgttcaacat gcaaaaaagc caaagcgtgg ttagaccagc atggcgttag ttatcaaacc
     3661 gttgatatga tcaaagaagt accgaaggcc tcaacgattg agacttggct agaaacgagt
     3721 caagtaccaa tccgtcgcct gttcaacaca agcgggatga aatatcggga acttggactg
     3781 aaagatcaag taggcgagtt tacggcttca aaagcgagcc aagtcttatc gacagatggt
     3841 atgctgatca agcgaccact gatcttacga gaggaccagc ttgttgcaat tggattcaat
     3901 gaaaaaattt atgaaggaga actgatataa aatggcaggc aagcatttga agaaaaagga
     3961 caacctttgg atcttattta atggaaaaga atactgtgtt ggtttgacaa atgaagcgca
     4021 agaagaactt ggagacatca cgtttgccaa tctgccaaaa gcaggacaat cgctaaaaaa
     4081 agatgaacct ctgatcgaag ttgaagcgga aaaagcggtc aatgaatttg ccagcccgct
     4141 aacgggtaca gtttcttctg ttaatgataa aatcggtgaa gatatcaata tcttgaatga
     4201 ttcagatgaa atgaatgcgt ggatcttaag tttcaaagat gttgatccaa aagaatttga
     4261 cgaattataa aatcagaaaa tcacttgaaa aaagttgaca ctgattcttc cagttgctat
     4321 aatcataagc aatcctatat gaaacgagaa agctttgaaa agaagagtac atgtcacgga
     4381 cgtttgaaga gagccctgtt tgctgaaaat gggtaacgaa aatgagatgg aagatggtct
     4441 tagagcagag taagcgagcg taagcaaact tactacggga gtgcccgtta cagcgcgaca
     4501 gtatacggaa agaagctaag tccaagtact ggaagaggct gttattgcga agtaacggta
     4561 aatcaaggtg gtaacacgga agttcaagct ttcgtccttt tgcttaaggc aagagggcga
     4621 aggctttttt gtttgaaaaa tcataagaga aagatgtgaa tggcatgtca ttaatccaac
     4681 taacgaatgt caaaaagaca ttcaatggga aaaacggaaa catccaagcc gtcaatgacg
     4741 tttcactaaa cgttgaaaaa ggggatattt atggaatcgt cggctattct ggcgcaggaa
     4801 aaagtacatt agtccgtctc ctgaatggac ttgagcttcc cacgagcggc gaagtgatcg
     4861 taaacgatca agatattaca aaattaaaaa acaaagaatt acgtacattc cgaaagaaaa
     4921 tcggtatgat cttccagcat ttcaacctgt tatggtcaag aactgtactt gaaaatatcc
     4981 agctgccgct ggaattagct ggcgtaccaa agagcaaacg taaagaacgg gcagaagaac
     5041 tgttgcgttt agtcgggtta gaaggacgcg gacaagcata tccttcacaa ctttccggcg
     5101 gacagaaaca acgcgtcggt atcgcacgag cattggcaaa tgatcctgag atcctgcttt
     5161 gtgatgaggc tactagcgca ttggacccgc aaacaacaga agaagtatta gacttattgt
     5221 tagcgatcaa taagaaattg aatttgacca tcgtcttgat cacacatgaa atgaatgtga
     5281 tccgcaaaat ctgcaacaaa gtcgctgtga tggagttggg gaaagtcgta gaagaaggag
     5341 acgtattgac tgtcttcaga caaccgaaaa aagaagtaac caaacgcttt gtacaacaag
     5401 acatcgaacc agaagctgat tcgacagaac tattagctga actcatcaaa gaaaatccaa
     5461 gcggccgttt ggcaactttg acctttagcg aaagaaatgc caacgagcca gtcatttccc
     5521 aagcgattcg acgctataac atcgatgtca acgttgtgta tggaaagatc aaacaagtga
     5581 aagaaggttc ctttggttca ttgactgtct tgatgactgg tgaaaccaaa gaattagatc
     5641 aagcagaagt gttcatcaaa gaacaaggag taggaattga ggtgatccat cgtggataaa
     5701 agcttttcag aaacatatct agattttaaa caaatcaacc cggaaacgat gaatcaggct
     5761 atccttgata cactatatat gacattaatc tcaatggtct tagtgacgat catcggatta
     5821 gttttgggat tgatccttta ctctattgga cgcaaaaaca aaccgtatgc tcgaatcatt
     5881 tatggaatcg tatcgatcat cagtaatatt ttccggtcaa cgccatttat gatcctgatg
     5941 gttttgatca ttccatttac taaagcaatc gtaggcacta tgctaggtgc gcaagcagcc
     6001 attccagctc ttgttttatc cgcagcacca ttttatgccc gtctagtaga gattgctttt
     6061 cgtgaagtca gccctggtgt actggaagca gcagatgcaa tgggagcaag ttactgggaa
     6121 atcatctgga aggtcttgat acctgaaagt attcccgcat tgatttctgg attaacagtt
     6181 acaacgatct cgatgatcgg atttactgcg atggccggcg cgatcggcgc aggtggactt
     6241 ggaggtcttg cttggcagga aggctaccag cgtggtaatc taacagtgac attcgttgcg
     6301 actttaatta ttttactaat cgtatttatc gttcaaggaa tcggtgattt cttaactaag
     6361 aaaacagaca aaagataata ggatataggg ggatgtagga tatgaaaaag aaaatttttg
     6421 gatttgcagc agcgcttttg ctgactgtag gattagcagc atgcggtaac aacagcgaca
     6481 gcaaagattc agcaaataaa gcagacgaca cgacattgaa agtcggcgct tcaccgacac
     6541 cgcacgcaga gattttggaa catgtgaaac cattattgaa agatgaagga atcgatcttg
     6601 aaatcgtcaa attcgatgac tatgtattac cgaaccaatc tttggaagaa ggcgacattg
     6661 atgccaatta cttccagcat attccttatt tgaacaaaga aatcaaagaa aaaggatatg
     6721 actttgtcaa tgccggtgct gtccacatcg aaccaatggg cttgtactct aaaaaagtca
     6781 aagatgtttc cgaattaaaa gatggtgcaa caatcattac ttcaaattct gaatcagact
     6841 ggggacgcat catcacaatt ctacaagatg ctggcttagt caaagtcaaa gatggagtag
     6901 atctggaaac agcaacattc gatgatattg aagaaaaccc taagaacttg aagttcaacc
     6961 atacgatcga cccatcactt ttagcttcta cgtaccaaaa tgatgaaggt gacctagttg
     7021 caatcaacgc aaacttcgct tacggcgcag ggttgaatcc attagaagat gccgtattgc
     7081 ttgaaaagga caactctcct tacgtgaata ttattgcgac tcgtaaaggt gatgaagaca
     7141 gtgacaaaat caaaaaactg attgaagtgc ttcaccgcaa agaagtccaa gaatggatca
     7201 cagacaaatg gggcggctct gttaaaccag taaaggctga tgcaaaataa ggtatggaac
     7261 aaaaaagatg agaaataagt ctgtaatggc ttatttctca tcttttttat acctattcta
     7321 atttagaaat caaaattgtt taata
//
LOCUS       DAYYCY010000125         7315 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000125 DAYYCY010000000
VERSION     DAYYCY010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7315
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.125"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            25..498
                     /locus_tag="ACHAZB_002138"
     CDS             25..498
                     /locus_tag="ACHAZB_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HFM8614774.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            495..2627
                     /gene="feoB"
                     /locus_tag="ACHAZB_002139"
     CDS             495..2627
                     /gene="feoB"
                     /locus_tag="ACHAZB_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HFM8614775.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            2629..2763
                     /locus_tag="ACHAZB_002140"
     CDS             2629..2763
                     /locus_tag="ACHAZB_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HFM8614776.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            2857..3501
                     /locus_tag="ACHAZB_002141"
     CDS             2857..3501
                     /locus_tag="ACHAZB_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8614777.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            3688..4881
                     /locus_tag="ACHAZB_002142"
     CDS             3688..4881
                     /locus_tag="ACHAZB_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM8614778.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            4874..6601
                     /locus_tag="ACHAZB_002143"
     CDS             4874..6601
                     /locus_tag="ACHAZB_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HFM8614779.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            6995..7192
                     /locus_tag="ACHAZB_002144"
     CDS             6995..7192
                     /locus_tag="ACHAZB_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFM8614780.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            7194..>7315
                     /locus_tag="ACHAZB_002145"
     CDS             7194..>7315
                     /locus_tag="ACHAZB_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFM8614781.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEK"
BASE COUNT         2380 a         1257 c         1627 g         2051 t
ORIGIN      
        1 ttatttcaca agagaggaat caatatgcag accttagcgc aggcaaagca gaatttcgtt
       61 tatcaagtcg aacgaatcga aacagaagaa aaaacaaaac gccatttgca aaatatcggg
      121 atcgttgccg gaagtaaaat cgtcgtcgtc aatttatcag gcaacaatgc cattttactg
      181 gtaaaaagtc atcgagtggc agtaagtcgg gaaatactag gtcaaatctt tgtcaataaa
      241 aagactgctt cctctgaaag ctggacttct cttgatcaat taaaagcagg agagactggg
      301 atcgtcgtag caatccacgg acaaggcgca gtgaagcgac ggttaatgga tatgggattg
      361 acgcgaaatg taaaaatagt tatccgaaaa ctagcccctc tgggagatcc aatcgaactg
      421 acagtgcgag ggtatgaact gacgttacga aagtcagagg ccgaactaat cttgatacaa
      481 agggaggaaa aagaatgaat atcgcgttag tcggaaatcc aaacagcggg aaaactagct
      541 tgttcaatct cttgactggt tcgaatcaat atgtagggaa ttggccgggc gtaacagtag
      601 aaaaaaaaga aggatcgttg aaaaaaaatc cgaacattca tattcaggac ctacccggca
      661 tctattcttt gtctccttat acaccagaag aagtgattgc aagagattat ttggttcagg
      721 aacagcctga actgatcatc aacattattg atgctaccaa tttagaacga aatctttatt
      781 taactacgca gttattggat ttagacattc ctatgatcat tggtctgaat atgatggatg
      841 tcatcaaaaa agaaggaaaa aaaatcaatc tagaaaagct ttcctatggt cttggtgtac
      901 cagttttacc aatcagcgta ttgaaaaagc aaggaatcga aaaactaatc gaaaagattc
      961 aggagtctgc aaaaaagcgg ccttctattc ctgttccctg tacatatgat ccacgtttag
     1021 aagcagcttt acatgaaatt gaagatgtac taggagaaca aagcggcaag aaacgctggt
     1081 atgcaatgaa atattttgaa cgagatcaga aggtaagaga ggattacgag atagcagttt
     1141 ctcagcaaaa agagatcgag caactgatcc agttaacaga gaaactattg gacgacgata
     1201 gtgagacgat tctggtcaat gaacgttatg aatttattac ccaactatgt acactctgtg
     1261 ttgtgtcaaa tgattctttc caattatcga tgagtgacaa aatcgatcag attgcaacga
     1321 atcgttggtt agccttgccg atcttcgcat ttgtgatgtg gctgatctat tatctggcta
     1381 ttcaaacagt aggaacaatg gggacagatt ggatcaatga cacattgttt ggtacatggt
     1441 taccagaaca tgttggcaga ttgttagctg aatggcaagt agcgggatgg atgcaggagc
     1501 tgatcttgaa cggcatcatc gcaggcgtgg gagcagtgtt aggatttcta ccgcaattga
     1561 ttatattgtt tttatgcttg agctttctgg aagactgcgg atatatggcg aggatcgctt
     1621 ttgtcatgga tcgacttttc aggaaattcg gtctttcagg aaaatccttc attcctatgc
     1681 tgattgctac aggatgtgga gtgccaggcg tcatggccag tcgcacgatt gaaaacgagc
     1741 aagatcgtcg tttgacgatc atggtcacaa ccttcatgcc ttgttctgca aagttgccga
     1801 ttattgcctt agtagcaggg gccttttttc ctcatcaatc ttgggtagct ccttctgcct
     1861 attttcttgg tatgactgca attattttct ctggaatcag cttgaaaaaa acacgtttgt
     1921 ttgctggaga tacagctcct tttatcatgg aattgcccgc atatcatttt cctcaatggt
     1981 cagcggtcct aagacaaacc tatgaccgca gcaagtcttt tgttaaaaaa gcaggaacga
     2041 ttatctttgt ttctagtatc gtgatctggt tcagctcttc ttataatttt tatgctcaac
     2101 ctgtttctga agaccaaagt atcttagcct ttttcgggcg aatcctggca gttgtttttc
     2161 agccgcttgg ttggggaaac tggcaaggaa cagtcgcagc gattacagga ttggtcgcta
     2221 aagaaaatat tattggcaca tttggtattt tgtttggtca tgcggaagta tccgaaaatg
     2281 gcagagaaat ctggcaagtg ctacaacaca catatactcc cgcagcagct tattcattgt
     2341 tagccttcaa tttactttgt gcgccttgtt ttgcggcaat tggggctatc catcgggaaa
     2401 tgaatgccaa gaaatggact tggatcgcca taggctatca atgcggttta gcttatttag
     2461 ttagttttgt gatttatcag ttgggacact tattggaggg tggacaggta gcgttaggga
     2521 cgtatcttgc tattttactg atgataggac tcgtttatgt tttgttccgt cagccaaaat
     2581 ccaagaacca gttttatact atattgtcat tagaaggaga ggaataggat ggcgacattc
     2641 ttattaagtg tgttaatctt tggaagtgca ggcgcaattg tctatactcg tttaaaatcc
     2701 ggtaagaatt gtgatgactg tcaaacagct tgcccagtaa aaaaggaaca gatcaagcga
     2761 tgatcagtcg ttgcaagagg ctattctccc cttttttgct tattttgcta gagcatagta
     2821 agatgaggga aagtaaaaaa agaaagagga aagaaaatgt ctggaaagaa aaaaatcgtc
     2881 cgcccgcgct accagcagat tgctgttgat ttagccgaac gtatcgttga aaaccgatat
     2941 aaagtgggtg agaagatcca tgcccgctca acacttgcca gtacgtacaa tgcttctcct
     3001 gagactaccc gcaaggctat caatgtgctg gtagatcttg gaatcatgga agtacggcat
     3061 ggaagtgggg catttgttgc ttcgaaggaa aaagcaaaag atttcctatt gcaatatcaa
     3121 gatgtacaat ccatccaaga tacaaaagca gaaatcatgg caagtgtgga gaagcaacaa
     3181 gatgagctga atcatttttc tcaattattg gatcagctgg tcaatcaaac gaagcgaata
     3241 cataagatga atcctttact tccgtttgaa ttatctttat cagaaaaagc aatgcatctg
     3301 gaccgttcaa ttagcgagtt gaatatctgg caggcgactg gcgccacagt tatcgcaatt
     3361 tctcataatg aagaactgct tgtttcacct ggcccctatg ctaaatttac ggcaggggac
     3421 atcgttcatt ttattggaaa tgaatataca ctacagcgta tgacaaactt tttttacccg
     3481 gagcatcagc ttgcagaatg attgtgtgaa atattaaaaa aatattaact tggctaaacc
     3541 ttttatttac aaagatttag ccaagttttt ttcagtttgg acaagcaaat ttctttcatt
     3601 tttgacaaag tgactacatg atgttaacat agtgtggtca cttttttcag aaagaaaaat
     3661 gaatttacat aaaaaaggag aatttgtttg gtaaaagtag aagtaaaaca tttaacaaag
     3721 atatttggta aaaagacaca agccgcttta gatatgatga atgatcacca gccgaaaacg
     3781 gagattttaa agaaaaccgg tgcaacggtt ggtgtgtatg acgtgaattt tgacgtaaaa
     3841 gaaggagaaa tcttcgtgat catggggctt tccgggagtg ggaaatccac attgatccgt
     3901 ctattgaatc gtctgattga gccaacttcg ggcagtatct atattgatgg agaagatgta
     3961 tcgaagttaa gcaaagaaga attgcgagaa gtccgtcgtc ataaaatcaa tatggttttc
     4021 caaaacttcg gtctgtttcc acatcgaacg atcttggaaa atacagaata cgggttagaa
     4081 gtccgtggtg tacctaaaga agaacgacaa gaaaaagcag aaaaagcatt agaaaattct
     4141 agtttgcttt catttaaaga ccaatatcct agtcagttat ctggtgggat gcaacaacgg
     4201 gttggcctag ccagagcttt agcaaatgat cctgagatct tgctgatgga cgaagcgttc
     4261 tcggctcttg atccgctgat ccggcgagaa atgcaagacg aattactgga cctgcaagcc
     4321 aatgttcaaa aaacgattat tttcattacc catgatttga atgaagcttt acggattggg
     4381 gacagaatcg ccttgatgaa agacggcgaa atcatgcaga tcggaacagg tgaagaaata
     4441 ttgaccaatc cagcaaatga ttatgtacgt gaatttgtgg aagaagtcga tcgttccaaa
     4501 gtgcttacgg ctcaaaacat catggtacct gcattgacga cgaatatcga atctgatgga
     4561 ccaaacgttg cattgacaag gatgcgtaat gaagaagtca gcatgctgat ggctgttgac
     4621 agaaagagac acctgaaagg gatcatcact gcagatcaag cgttagaagc taggaagcaa
     4681 aaacgtccat tgatcgactt cctagatgaa aatgtgacgg tgattgggaa agacatggtg
     4741 gtaagtgata ttttcaatat tatttatgac tcaccgactc cacttgcagt tgtagaggat
     4801 ggaaaattaa aaggtgtcgt catccgaggt agtgtgattg aagcattagc agaaacaagc
     4861 gaggtgagtg aacatgaata atctattgaa ctatcaatta ccagtggcaa actgggtaga
     4921 aaatatcaca gaatggttta cgacaacttt ttctggccta ttttcatttt tacaaacgat
     4981 tggacaggca gtgatgtcgg ggattacaaa tttactgtta gtgattccag ctcctttgtt
     5041 tatcttgtta ttgacggtag cagctttctt tatttctaag aaacgtccag gacttacgtt
     5101 gttcacatta atcggtctat ggtttattta taaccaaggt ctatggaatg atttgatgaa
     5161 tactgtcaca cttgttcttt tatctagtgt gatctcgatc atcattgggg taccattagg
     5221 gatcttgatg gcaaaaagca gtaaagccca aagtattatc aaaccgatcc ttgactttat
     5281 gcagacaatg ccaggattcg tttatttgat tccagcagtt gcatttttcg gtatcgggat
     5341 ggttccaggg gtatttgcct cagtcatctt tgctctgcca ccaaccgttc gttttacgaa
     5401 tttagggatt cgacaagttc ctaaagaatt agtagaagct tctgattcat tcgggagcac
     5461 aagtcgtcaa aaactattca aactagaatt gcctttagct aaaagtacga tcatggcagg
     5521 gatcaatcaa acaacaatgc tgtcactttc aatggtcgtt attgcctcta tgatcggtgc
     5581 acccggctta ggacgcggcg tattatcagc attgcaaaga gcacaagtcg ggaatggatt
     5641 tgtcaacggg gttgcattag ttatcttagc aatcatcatt gaccgtttta cacagcattt
     5701 gaaccagcca aacaataaaa aagctgcagg agcaacaaca cagaaaagta aaaaacgtca
     5761 aggactgatc attggtgctg tagtagtagt gatcttaggt gcgattggta tagggagttt
     5821 ttcatctgcc aaagaaacaa aacgtatcaa cttatcttat gttgaatggg atacagaagt
     5881 tgcttcaaca aatgtagtcg gtgaagtatt gaaacaaatg ggctatgatg taacgatgac
     5941 gccattagat aattcaatca tgtggaaatc tgtttcaaat ggcgagtcag atgccatggt
     6001 ttcagcttgg ctacctaaaa ctcatggttc acaatatgca caatataaag atcaagtcga
     6061 agacctaggt gccaatttga caggagcaaa agttggttta gcagtgccag cctacatgga
     6121 tgtaaattcg atcgatgagt taaccgatca ggcaggtaag aaaatcatag gaatcgagcc
     6181 aggagctggt gtagttacgg ctgctgaaaa cacgatccaa aaatatgaca acttgaaaga
     6241 ctggaaagta gagacatctt cttcaggagc aatgaccgtt gcactaggtc aagcgatcaa
     6301 aaaacacgaa ccaattgtag ttacaggatg gacacctcat tggatgtttg ccaaatatga
     6361 tctgaaatac ttagaagatc ctgaaaacgg aatgggaagc gaagagcaga tccatacgat
     6421 ggttcgaaaa ggattgaaag aagaccagcc agaagcttac aaagtattag acaatttcca
     6481 ttggtcagaa aaagatatgg aaaaagttat gttggaaatc aacaatggaa aagatccaca
     6541 gcaagcagca aaagactgga tcaaagagaa ccaagaatta gtcgaaagct ggaaaaaata
     6601 aaaagacaga cacgaaataa atggagcttc caaaattgga tcaaatatcc aaatttggaa
     6661 gctccatgtt cgtctctatc ttttttgtct ataaaaaaag agaatgcgta gctcatcttc
     6721 gaaacgtcca aagacgccga caaactatta tgaatttatg ggtgcgagtc aaagagatcg
     6781 atcgtaggta tgaaagacta atgtgattaa agtttattat gtacgaagtg cattctcaaa
     6841 aaatattaaa gaatatacat agtttatata cttaaaagtg attaaattct tattcaggaa
     6901 ttattttgct acgtttcaag aaggaattgt tttttgaaat ttaatgaatg ttagaataat
     6961 agtctaaaaa cattaattta ggggtgatta gattatgaaa catttaaaaa ttttgtctat
     7021 taaagagaca caacttatct atgggggtac cactcatagt ggaaaatatt atggaaatgg
     7081 agtgtattgc actaaaaata aatgtacggt cgattgggcc aaggcaacta cttgtattgc
     7141 aggaatgtct ataggtggtt ttttaggtgg agcaattcca gggaagtgct aaaatgaaaa
     7201 aaaatgctaa gcaaattgtt catgaattat ataatgatat atctataagt aaagatccta
     7261 aatattctga tattcttgag gttttacaaa aggtatattt aaaattagaa aaaca
//
LOCUS       DAYYCY010000126         7298 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000126 DAYYCY010000000
VERSION     DAYYCY010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7298
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.126"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..656)
                     /gene="nth"
                     /locus_tag="ACHAZB_002146"
     CDS             complement(<1..656)
                     /gene="nth"
                     /locus_tag="ACHAZB_002146"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFM8614782.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     L"
     gene            complement(663..1361)
                     /locus_tag="ACHAZB_002147"
     CDS             complement(663..1361)
                     /locus_tag="ACHAZB_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFM8614783.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            1629..2813
                     /locus_tag="ACHAZB_002148"
     CDS             1629..2813
                     /locus_tag="ACHAZB_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HFM8614784.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(2819..3262)
                     /locus_tag="ACHAZB_002149"
     CDS             complement(2819..3262)
                     /locus_tag="ACHAZB_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFM8614785.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(3488..3775)
                     /locus_tag="ACHAZB_002150"
     CDS             complement(3488..3775)
                     /locus_tag="ACHAZB_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFM8614786.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            complement(3946..5298)
                     /locus_tag="ACHAZB_002151"
     CDS             complement(3946..5298)
                     /locus_tag="ACHAZB_002151"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HFM8614787.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            complement(5571..6017)
                     /locus_tag="ACHAZB_002152"
     CDS             complement(5571..6017)
                     /locus_tag="ACHAZB_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFM8614788.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            complement(6253..7179)
                     /gene="cysK"
                     /locus_tag="ACHAZB_002153"
     CDS             complement(6253..7179)
                     /gene="cysK"
                     /locus_tag="ACHAZB_002153"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFM8614789.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
BASE COUNT         1952 a         1505 c         1209 g         2632 t
ORIGIN      
        1 agcgcctttt cttttagacg catccgattt ttcccatctt gacatattga tagcaaagga
       61 catacctcgc acttgggatt tcttgccgtg caatgatatc taccaaaaaa aatcagtgta
      121 tgatgtgtct ttacccataa ttcttgtggt actttccgca tcagtgtttc ttctacttcc
      181 atgaccgtag catctagttt gcagatcctc aggcgttttg agacacgttc cacgtgggta
      241 tctactgcga ttgctggtat cccgaatgca tcccccaata cgacatttgc tgtctttctg
      301 cctacaccag gaagagacat caattcttct cttgacgttg gcacttgccc atcgaaacgt
      361 tcaatcagct gctgtgcaca cgctttgatg ttttttgctt tatttcgata caaaccaatc
      421 gtttttattt taagaatgat ttcatcgatt gaagcttctg ctaacgcatc gggtgtcgga
      481 aaagaggcaa acaaatctgg cgttgcttta ttcactgata catctgtcgc ctgggcactt
      541 aagatcactg caatcaataa ctcaaatgga ttattgtgct tgagttctcc atgtgcttcg
      601 ggaaacattc catacatcgt ttctagagct tccattgttt tttgtttgct taacatattc
      661 cgctactcct ccaaccaatt tttcagtgag actttcggca attcttcttg ttgagctgac
      721 tgttctactt ctctttgaag aagctgctgt tttcttcgtt tttggtcttc ttccacttgt
      781 tgttttgtac gtagattttt tctttcccac gatagcaaaa tgcggtcaac gtaattaaaa
      841 ctataagcct gatttaatac ggcttcccgc agtgctagct tcaaaatttc cggctgataa
      901 tggtcttctt ctagccattg tccgatccgc tggaactcga tcgaagaaag cgggcgtcca
      961 aattcttgtt caaataattg atacactgac tggatttctt tttcttgtgt tttttcttcg
     1021 tatttttttt cttgtgtttt taaatactca cctaatagat catagatggg gtaaagatta
     1081 tatcgatctg cttttttacc gttgacaatc gtcgtttcta tcttgataaa tccatttgtt
     1141 acgagacgat ccagtatttg aaaaatctga tcttgtttga ttcccatatc catagcaatc
     1201 atctgcaaat cagggaaagt atctccttca agctgtgcca tatgcaactg tagaacaaat
     1261 aaaaattctt cagattgtag accgatccta tgataatttt taagtaaaag attagatatg
     1321 actgtttgtc cagcgcttaa gtaattttct aaattcaaca tgatttctcc tccgctcaat
     1381 cacagtatac gtaaaaaaaa catgggaagc aagaacaggt ttcttttcta tttacttcac
     1441 tgatcggtca gatttacaat gtcgtctact tgatttttgt ttgttcatgt ttttgatgcg
     1501 tatcttcact tttcggaata aataaattaa gtagatttcg ctgttcctta gaatgttgac
     1561 aaggaaacaa aattactata cactattttt gtttccaagt taacattatt gcataaagga
     1621 gtttttacat gtacagtttc acaaataaac attctaaagt ttttggatac gtctctgtct
     1681 tattcactgg attaggattt actatcgtca gtcctgtact tccgttttta acgctgcctt
     1741 attctcactc catacatcag caagctttct atatcacttt gctcatgtct gtttatgctc
     1801 tagctgcctt cttatctgcc cccattctcg gtagcttaag tgatcatttt gggcgtcatc
     1861 cgatccttat tatcagttta ttaggatcaa gtatcggata tttgcttttt ggtttaggaa
     1921 attcgatatg gatgttgttc cttggtcgta tcattgaagg attgacggca ggagaaatct
     1981 ccaccttata tgcttacttt gccgacatca cggaacctaa tgaaagaacc aaagtttttg
     2041 gatggatggg tgctttagtc ggtataggaa caacgctggg tcctattata gggggtctat
     2101 tagctgagtt ggggaattct gttcctatat ttattggagc actctttact ttcttaaatg
     2161 ccgtttatgg ctacacattt atgctagaaa gccttccaat aaaaaaacgt tcagtagatt
     2221 taagtttctc ccatgtaaga ccttttcacc aattacaaca attattcaag atttcatccg
     2281 ttattccttt attgacagca ggttttgctg tttggttggc agctggctct cttcaatcta
     2341 tcttttctca attttcgatt gacgcctttc agtggaaggc tgggttggtt ggtttaagct
     2401 tctctttgat tggaatcttg gatactgtct cgcaattgtt cattatgcct cgattgctca
     2461 aaaaattttc agaacagcag atcacaagaa tagggatgtt cagtgaaatc ctcgcctatc
     2521 tattcatcac attatctgga attcttctac tgccgatttt gtttttattt gggattatct
     2581 gttatggttt cggtgattct atttttactc ctgtattcaa tggccaacta tccaattctg
     2641 tctcagataa ccagcaaggt cttgtaatgg gcggtaccca aagtatacaa tctctatcgc
     2701 gcgtcatcgg cccgctaatc gcaggacagc tttatgctgc agcaccattt cttccgacgg
     2761 tattcggatt tttgcttatg atttttgcct attatcacta caataaaaaa tagtagcttc
     2821 atttgctact tcgtataaat tctctgtatc gaccgataaa acgaaatact gtgtcaaatt
     2881 cttcttttga catattttca aatacagtag catctcgctg tacccatttt tgatggagtt
     2941 gctcgtgtct attccttatt ttttgtccga ttggcgtcaa ttgaaaatag atttcttttt
     3001 tattctcttc gctttgataa gtgtttatga tccctctagc tatcagtttt tttgttagct
     3061 tactgatggc tcctctagtc atgtaactag cattcgctaa ttttgtcacg ttaggatcag
     3121 ggattttccc gatatgttca atcacttcta gttcatttag agtaaatcct gcaaattctt
     3181 tttccatttc tggtttatta cgatgaataa tctcattagc taaatccaga tattcttcta
     3241 ttagcagttg tcttttatcc atttctacac cttctttttc tttcattata ttgttttttt
     3301 gtttcctatt caacaaagat aaaaatattt tgtaggaaaa cccaatctga aaccaagaaa
     3361 aaaaggggtt gacaaagctt gtcataaccc ctttttctga ttaaaagata gtaaagagac
     3421 agtcatcggt aattgtgatg attttttgtc atcacttaag gatgagcggc cttttcaaaa
     3481 actgtcttta aagatcatca tcagtttcta gcacatatgg ctgaccttca tatgtgtact
     3541 ctgtattcaa tgtgccgtca ttttcatctg agtagatgcg tctactgaaa ggaaaacgat
     3601 gagaaacatt atctttggcg tcatttgctg gcatcgtgta atattcctct tgcgtttcat
     3661 tcaactgaga gagtacttga tctgccaact catcaatatt gatcgttaat ttcttcgaag
     3721 cacttttata atcttctaaa aatgcatctt gttggacaaa atattcttta ttcatcttct
     3781 ttctcacctt tcttttcaaa atcaaataaa gctgcttgtt cttttatgta acagctttat
     3841 ctttgctgct tattgtgaca aaaaattcga agaaagacaa ctaattgtac ttttcacaaa
     3901 aaaagacaga gggtcgtgac aaaattgtgt cacaaccctt tttctttatt gtgcagtttc
     3961 gatttcgcct agcgctgctt gtgcaagtag gttcaagtaa ttccatgggc ggtcaaaatg
     4021 tggttggaag aagaaatctg aaagtgcgag atcttctaca gtcattttat tttgaacagc
     4081 taaagacatg gtatttgctg attgtgtaat gtcgtatttg gacatgaact gagcacctac
     4141 gatacgattc gttccttttt catagactag ctccatcatt actttttcag tagttggcat
     4201 gaactctgga cgataattat cttcaaatac agtcgcttgg acttctagat cgttcaaagt
     4261 tgcgctttct ttggtcacac ctgtcgaacc gatcgtccag ccaaataggt aaagtcctga
     4321 agttccttga gtaccacgat acgccatttt atgatctgtc aagttatttc ccacaagcaa
     4381 accttgacga acggcatttg ttgccaaagg aatatagttt tgcgtgccac ttggattgta
     4441 gttgactact gcagagtctc cagctgcaaa aatgtctgga ttacttgtct gcatgtattc
     4501 attcactttg attgccccgt ttggcagtgt atctactttt ccttcgatca gttttgtatt
     4561 tggacggaag cccacacata aaatgaccat atctgtatca aatgtatctg ttggtgtagc
     4621 tacttgtttc acttttcctt cttcatctgt gataaattca gtaacatttt cgcctagcgc
     4681 aagagtaaca ccgcggtctg tcaattcttt ttctaagata tctgtgaatg gtttatctag
     4741 atatttattc aagatgcgat ctaatccatc aatcaatgtc acttgttttc cagattcgac
     4801 aaaagcttct actaattcaa taccgatata tccaccacca acaattacta cacgttttgc
     4861 ttctttcgct tgtgcaataa tttcatttgc ttgattgtag tttttgcata ggagtacatt
     4921 tttcgcatcg attccactaa tcggtgggat aataggccat gaaccagttg tcatcactaa
     4981 tttatcataa gaaaccgttt ctgtttctcc tgttttcaga tcttttgccg ttactgtttt
     5041 attttctgta tcgacgtctg ttacatcgtg ttccatttta actgttgctc ctaatgaaga
     5101 taattcttca ggatttgagt agaacaagcc tgctgggtcc tttactactc cgccgacata
     5161 aagtgcgata ccgcaagata agaaagaaat attgtcattt ctttcatata ctgtcacttc
     5221 tgcttctgga tgatttttta aaatactttt tacagctgca gtacctgcat gagtacagcc
     5281 aattactact actttcattt atggtccctc cgattaacat tttgtccttc gacaaaacta
     5341 ctataacaga aataaaacgg atacaacagt ttaatgcttg tgaattcaca aaggtttgtt
     5401 tgtagtttca ttccttttca agcataagaa cccaatatct acaagattct tattttaata
     5461 atattcactt ttatatctaa gattgtgaat aaaagacaaa aaaataaaaa agctagcgtt
     5521 ttttttacac agctagcttt ttttaggtat aacacttgta gaaactgttt ttacatttgc
     5581 gccttttttg ctgactcttt acaatccgga cagagtccat acaattccaa ccgatgttca
     5641 ttaatctcaa aatcagtcag tctgcttgca gccatttcta tatcttcaag ccctgggtaa
     5701 tgaaaatcaa caatcttacc gcagttttca caaattacat gataatgacg ctttgaactg
     5761 aaatcaaatc ggctagaagc atcgccgtac ttcatttctt gaacaaatcc aatcgaagta
     5821 aataagcgaa ggttattgta aacagtcgca acactcatat ttggaaaccg atgttcaagt
     5881 gctttataaa tctcatcagc cgttggatgg gaatgatgtt caatcaaata ttctaaaacc
     5941 gcgtaccgtt gaggtgtgat tcgaatgttc tcttttttta acttttcgat aacctcttcg
     6001 actaacttat tttccatgta aacaccccac gttgttattc tcgtcttatc atactatttt
     6061 tacattttaa aatccactta acatgaaaag aaaattcaat tttattgtat tttaatgaaa
     6121 aaagacgtaa aacagtcgcg ttcactattc tacgtctcta ttcaaataat ttttctcatc
     6181 ataaattttt gtgtgttctt ttcatcttta aaagtttttt acataaacct atctggagaa
     6241 aaaccaagtg ccttatatct cttggtaaag ggctgtagat aaataacgtt caccattgtc
     6301 gggaaccaca gctaatacct tcttgccttt tcctaattct tttgcgactt ttagcgcggc
     6361 agcaattgca gcgccagatg agatgcctac taaaatccct tcttttcgcc caacttctct
     6421 agctgtttcc atcgcttcgt ctccactaat agaaaggact ttatcataca cagaagtatc
     6481 cagtgtttta ggaacaaaac ctgtaccgat tccttggatt ttgtgaggtc ccgcttcttt
     6541 cccttctaaa atagcagatt ctgctggttc taccccgatt aattcgattt tcggatacac
     6601 acgttttaat tctcggccag cgccagtgat cgttcctcct gtaccgatac ctgcaacaaa
     6661 cgcgtccaag ccatttactc caaatgcttg atgtatctca ggaccagtcg tcttctcatg
     6721 cacaagagga tttgcttcat tttcaaattg taatggtaaa aagtagccgt tttcttttgc
     6781 caaacgttct gcttctctga ttgatccaga aataccatcc gcaccaggag tcaaaatcaa
     6841 ctctgcgccg taccctttca tcaataaacg tctttcgata ctcatcgtct caggcatcac
     6901 gatgattact ttatatcctt ttgcaactcc taccatcgac aatccgattc ctgtatttcc
     6961 agaagtaggt tcaatgatag tatcacctgg tttcagtaga ccatcgtgtt ctgctttttc
     7021 tatcatactt aaagcaatac gatcttttac acttcctcct ggattaaaaa actcaagctt
     7081 tacaaaaaca tctgctgaat cttctggtac gattttattt aatttgacga ttggcgtttt
     7141 gccaatcaat tcggtgactg agttatagat agctgtcatc ttcaattcct ccttgtcttt
     7201 gctcttctta cttatcataa tacaatctgt tcatttatgg cgagtgattc gtctttgact
     7261 agcaaaaaaa agacaaagat ataccatata tggtaatt
//
LOCUS       DAYYCY010000127         7042 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000127 DAYYCY010000000
VERSION     DAYYCY010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7042
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.127"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            51..680
                     /locus_tag="ACHAZB_002154"
     CDS             51..680
                     /locus_tag="ACHAZB_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8614790.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(804..1913)
                     /locus_tag="ACHAZB_002155"
     CDS             complement(804..1913)
                     /locus_tag="ACHAZB_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8614791.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            2122..2544
                     /gene="mgsA"
                     /locus_tag="ACHAZB_002156"
     CDS             2122..2544
                     /gene="mgsA"
                     /locus_tag="ACHAZB_002156"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFM8614792.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     regulatory      2731..2829
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            2867..3403
                     /locus_tag="ACHAZB_002157"
     CDS             2867..3403
                     /locus_tag="ACHAZB_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFM8614793.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(3443..5206)
                     /locus_tag="ACHAZB_002158"
     CDS             complement(3443..5206)
                     /locus_tag="ACHAZB_002158"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8614794.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(5209..6924)
                     /locus_tag="ACHAZB_002159"
     CDS             complement(5209..6924)
                     /locus_tag="ACHAZB_002159"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8614795.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT         2105 a         1359 c         1395 g         2183 t
ORIGIN      
        1 ttatactaag tcgacattta tgtgtcaaga catattagga gagagaaaaa atgagaaaaa
       61 attcagttcg aaaactaacg atttctgctt tattgattgg gatgggggtc attatcccaa
      121 tggtcatgcc taaaatcatg attccaccag cttcctttac tttggcgtct catgtgccat
      181 tatttattgc gatgtttttt acaccaggag ttgcagtggc tgttgcgcta ggtacgacat
      241 ttggtttctt tttaacaacg ccggttatca tcgcattgcg agcattgtca catcttgtgt
      301 ttgcgttgat tggggcttgg tacttgcaaa agcatccgag tatcgtttta aaaaatggac
      361 agtttacttt tgctaattgg cgtttccaag ggttcaattt tgtgatcggt gtgattcatt
      421 ctttagcaga aatgctagta gtcagcatct tttactttat tggtaatatg cctgaaacat
      481 attattctca aggatatttt tatacagtat tcatcttgat ggggattggt gggcttatcc
      541 acagtttagt tgattataat attgcttatt ttatagcagg aacactcagc aaatattttg
      601 atatccccgt atttacggca gcaaaaaaag aaaatcggaa agagtcgatt caagggtacg
      661 cgaagaaaac gacatattag acatgatctc tcaaaaaaaa aactctcatc tagtaatgga
      721 taataaatcc tctaagatga gagttttttg tcctaaaaag agctgcacca acatcttctg
      781 gcacagctct aacagggggc aatttatgga agtttgatga cttgttctgt ttctttatca
      841 aagaagtggc ctttgctgat gttgaaggca aggtcgacta tttcgcctgg tttatggaaa
      901 tctcttgcat ctacttttga gatgaactca gtagaaccag tttttgtgta aagcatcgtt
      961 tcagctccaa gtaattcgga aacgacaact tctgatttca ctacggcttc aggtgaagct
     1021 tcaagggcta cttgttcact gtggatgtct tctggacgaa taccgaagat aacttctttt
     1081 ccttcataac ctttttcaac taatactttg ttttttcctt caggaatacg aagcttaagc
     1141 ccatgtccat ctgtgataac accatttttc aatgtcacat taaagaagtt catggcagga
     1201 gatccaataa agccggcaac gaacatattg acgggtgtat cataaacttc tttcggtgta
     1261 ccgatttgtt ggataaatcc gtctttcatg ataacgattc ggtcagccat agtcattgct
     1321 tctgtctgat cgtgagtaac atagatggtc gttgtttcaa gacgctggtg aagtttggca
     1381 atctcagcac gcatggctac acgtaattta gcatctaaat ttgataatgg ctcatccatt
     1441 aagaagactt ttgcatcccg aacgattgcc cgtcccaatg ccacacgttg gcgttgtcca
     1501 ccagataaag cagctggttt tcgtttcaag tattctgtca gacctaagat ctctgctgca
     1561 ttgtccacac gttttttgat gtctgcttta tcatatttac gtaatttcaa gccaaaagcc
     1621 atattatcaa aaacagtcat atgaggatac aatgcgtagt tttggaaaac catggcaata
     1681 tcacggtctt taggtgccac gtcattcatg acttttccgc cgatatttag ttctccttca
     1741 gagatatctt ccagtcccgc gatcattcgc aaagtagttg actttccaca accggaagga
     1801 ccaacaaaaa cgataaattc acggtctttg attgatagat taaaatcagt tacagaataa
     1861 ttttctgcat tgtcatattt tttatatacg tttttcagag ccatttctac cataatacgt
     1921 tccacacttt cttttttagt tttattaact atttaaagta taaatgaaag cgttaactaa
     1981 cttcaatgtc atgttgaaca agaatttttt cttttttttg tcaatgtgct agttttaagc
     2041 tttaataaat atccataaga aataaaaact gatacactgg agtaaataag tatgttaaca
     2101 atatcaaaag gagtgtctct tatgaaaatc gctttgatcg ctcatgaccg aaaaaaacct
     2161 atgatggtga aattagcaac agcgtataaa acaatattgg aaaaacatga actttttgct
     2221 accgggacga ctgggcagag gatcacggaa gcaaccggct tgccgatcca tcggtttaaa
     2281 tccggaccgt taggcgggga tcagcaaatt ggagccatga tttcggagga tcagttagat
     2341 ttggtgattt ttcttcgaga cccgctggct gcacagcccc atgaaccaga tgtaacagca
     2401 cttatccgtt tatctgatgt ctatgagatt ccgttagcta cgaatatcgg aacagctgag
     2461 gtgctactaa gaggactgga agccggcttc ctagattttc gaagaatcgt ccatgaaata
     2521 gacaagcatc cactttcatt ttgatggata agcgtatgga aaaataacgt ttaatgttcg
     2581 cctatatgta gctataaaag ctaaacaaac tgataatatg cgatataata cgaacgtatt
     2641 ggataatata aacaataata ttagtttttc gtatttgcag taaatgtgaa aatatgctat
     2701 agtttctgtg ggaaaagatc ccaaaaataa tacagagtag aaaataaagc gttaagtgct
     2761 aagaggatgg gatgttgcct cttggacgaa ggacgtaatg ttcgcggttt tattttcgca
     2821 ttcgctgcat gatacgatgt agcagctctt aaaggagatg ctaaaaatga agaaacaccg
     2881 gttagacgca cgtatgattg ctattatggg cttattgatt gcattgatgg tcactttgtc
     2941 ccgactggta gcaatcgaga ccccatttat taaaatcagt gtcacattta ttccacaagt
     3001 gatcatgggc attctttttg gtcctttttg gtcagggata ggcgcagttt tagctgattt
     3061 agtaggaatg gccctcttct cgaaatctgc attctttatt ggatttacat tgaatgcatt
     3121 tattgaagga gcaatctacg gattcttttt ctatcgaaaa gagatcactt ggaaaaatgc
     3181 cattctggct acgcttagtg tcacgctgat catcaactta tttttgacac cattatggct
     3241 tgcgttgatg taccatgtgc cattgttcag ctgggtcgtt tgggcgcccc gcctgttaaa
     3301 aacagtaata tggttaccga ttcaatcaat tgccatttat tacgtaggac gtagtattcc
     3361 ttacaaaaaa attttaagaa gtttagcgat ccacgctaaa taaaaaacaa gcagtcttcc
     3421 catcccagaa gactgcttgt ttttattgtg ctgtgaattg tgcttgatag agccgtcggt
     3481 aataaccatc tttttgaagc agctcatcat gggtgcccat ttcgacaata gctccctgat
     3541 ccatcactaa tatctggtcc gcatttttga ttgtggacag gcgatgggca atcacaaaac
     3601 tggtttttcc ttccatcatt gctaagaagg cttgttggat ttttttctct gtcaatgtat
     3661 cgactgagct ggtagcttca tctaagatca acataggggg attactgatc atcgttcgag
     3721 caatcgtcaa cagttggcgt tgtccatctg atatctttaa cccttgaccg ccaatcttag
     3781 tatctaattt ttccggcaaa cgttctacaa attcatacat ataagaggct tttagggcat
     3841 cattgatttc tgcgtcactt gcttccggat tcccaaaacg aagattttct cgcaatgtac
     3901 tgtcaaatag ccaagtatct tgtaacacca ttccaaaact tttacgtaat gtatcacgtt
     3961 ggatagagcg gatgtcatgg tcatcgactg taattgttcc ttttgttgtg tcataaaaac
     4021 gcatcagcaa attaacaagt gtggattttc cggcccctgt ttttcctact atagcaatgg
     4081 tttgacctgg ataagcagta aaattgaaat cttcgatcaa acgctgcttg gggtcataag
     4141 aaaaagaaac atgatcaaaa gagacttttc ctttgatatt tttgggttcc aaggcatagg
     4201 catctggcaa atcaggcttt tcttctggct gatcgatcaa atcaaatgct cgttctagac
     4261 cagaaaaagc ggtttgcagt tgtgtcgtga ttccagaaag ttcgataaat ggtttagaaa
     4321 actgacttga gtagatcgta aagctggaaa tcacaccaac agtgattgac gaaccactct
     4381 tcaatgccaa taggccgcca actaaaccaa tcgataaata agcaaggtga tctacaaaac
     4441 gagaagacgg gtttgtcaag gaagaagaaa attgtgcccg ttgtcctttt tcatatagat
     4501 cagcatttag cagttcaaat tgctgttgac tagcttcttc ttgctgaaaa gctttcacga
     4561 ttttctggtt accgattcgt tcggtcacaa agcccgagat atccccaata atcctttgct
     4621 gtctatcgaa atttgtttgt gaagcacgag caacgatcca actcacaagg aaaataattg
     4681 gtgtagaaca aagaacgacc aatgtcagaa gtggacttag acgcatcata aaaataaacg
     4741 aaagaatgat gattgccact ccagaaaata attgattgaa ggccgcagaa attgcaatgg
     4801 aaatattgtc catatcattt gtaaatcgac tgacgatatt tccgtgggag ttttgatcat
     4861 aataacgaag tggcaatcga ttcagatgag caaatgcatc ttttcttaat tttgcaacgg
     4921 ataagtaagc taaccgattg cttaacactt ggatgagcca ttgactgact acgttgatta
     4981 aaacgatacc accgaataga ataagtaaat ggaacagtcg attgaagcgg acctgtcctt
     5041 ctccaatcat ttgatcgata ctttgaccaa tttgtaacgt catcacaact gttgcaatcc
     5101 cattgatgat tcctagtatc acagctagcc aaatgtcttt tgcataggca gtaaggtaag
     5161 gaatgaatcg gcggagcgtc tgcaaggata atttttttac tttcataact attcttcctc
     5221 ctgtgatgcg acgatttcct gatattctgc tgattgagca agtaattctt catgggtacc
     5281 ctgagcagca atcttacctt gatccatcac taagatatga tgtgcttctt gaatagagcg
     5341 gacacgttgg gagataataa tcactgtact gccaagataa gtctgtagcg ccttacgcaa
     5401 atccaagtct gtttgataat ccaacgcact taaagagtca tctaaaatca gtaatgctgg
     5461 cttggcaatc aaagctcttg caatcgtcag tcgctgtttt tgtccgccag agaaattttt
     5521 cccattttcc atgacttttg tatctaaacc atttggaagc tgacggacaa attcttctgc
     5581 ctgagcgatt ttcagagctt cccaacattc tttttctgtg gcgtctttct taccccattg
     5641 aaggttttca cgaatagtcc cggtaaatag tacagaagtt tggggaacaa gagcaatttg
     5701 ctgacggaga tgatccagct gccaagaacg aacatccatt ccgtccatta taactgtacc
     5761 ggaactgaca tcatagaaac gcggaatcaa ttgtgtcaaa gtcgttttcc cgcttccggt
     5821 tggaccaatg attccaagga ctgtatttcg cttcagctga aatgtaatat tttggagaga
     5881 aagaccagaa tcaggagtat aacggaagtc aacatggtca aaagcgataa ttgcttcaga
     5941 agaaaaatcg ggagtagtac tgacctcagc ttcaagaatc gaattttctg tgtctagcac
     6001 ctctttcacc cgatttcctg aagcttcggc acgtgtgaag atcacgacga ggtttgaaac
     6061 aacgatcagt gccaaaagca tttgattcat ataattgatc aaagcaagaa cttgtccttg
     6121 ttgcaagcta ccaatattta ctttgatccc gccaactgtc agcaaacaga tgatcccgac
     6181 attcatgatc aaggttgtag caggtgataa tagggcagag atattggaaa cacgtaaata
     6241 attgttcgct agatcatttg tgcttttatc gaacttctct gtttcttttt ttgtctgcgc
     6301 aaatgcgcgg atgacacgaa caccgctaag attctgacta acgtgttcat tgagctgatc
     6361 tagtttttct tgcacttttt ggtagagagg aacggttttt ttgatgataa aaaataaaat
     6421 gattgagaaa ataggcaata atgctaagaa aaacagacct acttcccagt cgatcacaaa
     6481 agccatgacc actgacccga tacttaaaaa cggtgctctg atcaataaac gaatgaccat
     6541 tgctaaagct agctgaagct gattgatgtc gtttgtcatt cgggtgatca aggtatctgt
     6601 tccaaaatga ttcaactcgt tgtgagagaa agtattgatt tttttgatta gctgattacg
     6661 taactctgta ccaaatcctt gtgaagctac tgaagcatag tattggcaaa tgatagcaca
     6721 aatcagacca atcacagaca tagcgaccat ccataagccc atttcaatga tttttcctcg
     6781 gtcattcatt tttagtccat tatcgatcaa ggaagccatc attaaaggca ggacaagttc
     6841 gaagacagct tcaagaaatt tgaagaacgg gcccaaaatg atttgcttac gatagtcttt
     6901 tgcataacgt aataactgaa tcatatgatt cctcctatta tttatttgtc cacaattgaa
     6961 aaaacaagta ctaccacatt gtagcttagg aaatttagaa tgaaaagaga acaagccttt
     7021 tcataaatga aagttttatt at
//
LOCUS       DAYYCY010000128         6981 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000128 DAYYCY010000000
VERSION     DAYYCY010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6981
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.128"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..187)
                     /locus_tag="ACHAZB_002160"
     CDS             complement(<1..187)
                     /locus_tag="ACHAZB_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8614796.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISL"
     gene            complement(324..737)
                     /locus_tag="ACHAZB_002161"
     CDS             complement(324..737)
                     /locus_tag="ACHAZB_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HFM8614797.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1193..1678)
                     /locus_tag="ACHAZB_002162"
     CDS             complement(1193..1678)
                     /locus_tag="ACHAZB_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8614798.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(1817..2032)
                     /locus_tag="ACHAZB_002163"
     CDS             complement(1817..2032)
                     /locus_tag="ACHAZB_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HFM8614799.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2256..3056
                     /locus_tag="ACHAZB_002164"
     CDS             2256..3056
                     /locus_tag="ACHAZB_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM8614800.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIKEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3075..4943
                     /locus_tag="ACHAZB_002165"
     CDS             3075..4943
                     /locus_tag="ACHAZB_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFM8614801.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            4936..5427
                     /locus_tag="ACHAZB_002166"
     CDS             4936..5427
                     /locus_tag="ACHAZB_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFM8614802.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            5415..5969
                     /locus_tag="ACHAZB_002167"
     CDS             5415..5969
                     /locus_tag="ACHAZB_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFM8614803.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            5987..>6981
                     /locus_tag="ACHAZB_002168"
     CDS             5987..>6981
                     /locus_tag="ACHAZB_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFM8614804.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVPTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2448 a         1114 c         1353 g         2066 t
ORIGIN      
        1 acaatgagat accagagtcg taataataaa atcctctaat cttatggata aaatagatca
       61 tcggacaagc agataagagt aagtatttgt taggcctttt gaataagtaa aagaaagcac
      121 tggtcattat gataaatggt atccaaaaca aaagtttact taagacaatc aaatgatcaa
      181 ctcccatatt ttttaccaag agagatcata acatctttat cgtaaaaaca aaatattttt
      241 ttgctcattt tgaaaattta aacatactcg ttcttatcca aatatataaa aatcgcccct
      301 cattcatcga gaggcgataa gttctaattt aactcaattt tttttgtttc ttttggctga
      361 taagctgttt taggtaatac caatttcagc acaccgtttt taaaggttcc agtgattttt
      421 tcttcatcaa cattaggaag atagaatgaa cgtttgtaag agaccgaatg tctttctttc
      481 cgtacatagt tgccatcttc tttatcttct gtatgggact catgattggc ggagatcgtc
      541 aaagtattat ctgaatattc aactgtcgtg tcttctttgt cacagccagg catatcagca
      601 gttagtttgt attctttttc attttcgact aaatctactt ttggataact tgaaacacct
      661 aaaaaatcat tgaatgctgg actaaacacg tcattgaaat cagggaacat gtcgcgaatg
      721 ctaggtacat ttgccatttt tgatcatcct ttcaataagt attttttatt tatccctatt
      781 tacgtaataa ctctattttg aaaaaaatgc aagtataatg gttagctttg tgaatatttc
      841 atctaatacg atacaaaggc tatctttcta atttgaaaaa gggatcatta aaataataag
      901 aagaaaatat gtcaaaaggt aaaaaagtaa attcttctat ccaaatatag aaaaattgaa
      961 tctagaaaac cgcccgacaa taaatttcat taactcagtt gaccgaaaat gaagtagata
     1021 ccaaaaattt atcaagagag caagtagaga agtgagtata ttcataagag aaatagctgt
     1081 aacgaattat taggttaatg caaatgctta tttatactcc gtataacttt ctcagaaaaa
     1141 atgataccaa taatgacttg ctagtactgt aaatccgtat aatatcatct aatcaaaatg
     1201 gtgttccatt tgcatctgtc atcgtaatcg atccttcggg tgacactcga taaaaaccaa
     1261 cgcttccaga tcctccttgg gaacggattt gtttgctgga agctgtgaac aaataatctc
     1321 gatcaatttt ttgataaaag ctaataacaa tatcgtcact ttgtaattcg gaacggtggg
     1381 caataagaaa tagaagtgct ttgtcagagt tttctaatgg aagagaaggt acagtgcttt
     1441 cttgcatttc agatgttgaa gaaatgctaa acgactgaat acttgattgg ctatgttgcg
     1501 aatctacatt accagtagaa gtattagtaa tggaagtagt agaagtactt ttagttgaac
     1561 caacctcagt tgttgaaatg gctgctgaag attctttaac agaattcttt gttttatatc
     1621 cacaggcaga aaaaagtatt acagttccca acaagcctgc gtaaatccct ttatgcatga
     1681 tagctcctcc taaaatagat tatctttatt cagtatatca cttaaacaaa gaaaagcaga
     1741 tggtttaaaa tgaaaaaacc ttcccaataa agggaaggtt catgaagata ataacttttt
     1801 taataattat agctttctaa tatctttcat caacttttct agtaatcatt gggaaaaatt
     1861 tcaagcagta gatcgcacaa atacctacga cgatataaat gatcttcgca aaaatagttg
     1921 ttgaaccacc agcaatcata gctactaaat caagttcgaa caagcctacc aacagccagt
     1981 tcaaccctcc cacaatcaat aaagttaatg caatagcatc taaagttttc attagaattc
     2041 tcctttctta aatgataaaa caattatata tcaaaaagag aaacattgct tatataacgc
     2101 tcaggaattt tagcaatcta tgcaaaaaga tgaaaatttt ttttgctctt ccattagtac
     2161 acatttttta gctgaagctg taaatttcat gtcttaattt tttttagtga gcttttatac
     2221 tggatttgta agaaaaaaga aggagaggat cacatatgtc ggattggttg gatattgcag
     2281 gaaaagtagt aattgtaact ggaggttcct caggaattgg aagaagtatc gtcgaaaatt
     2341 tgttgaagca gaatgcacaa gtagcgaatt ttgatgtaac tgaatgccgt atacagcatg
     2401 aaaacctact atctttaaaa gtcgatgtgt cttctaaaac ggacataaaa gaagggatat
     2461 ataaagtaat gaaacatttt aagacgatcg atggtctagt caataatgca ggaataaata
     2521 ttccttctct tctaatagat agaaatcatc caaaaagcaa gtatgaatta tcagaacaag
     2581 tttttgataa gatgatcgct gtcaatcaaa aaagtgtata tttgatgagc caagcagttg
     2641 gaagaatctt ggtgcaaaag ggatcaggag tgatcgtgaa tctttcttct gaatcaggat
     2701 tggaaggatc agaaggacag agctgttatg ctgctacaaa agcagcgatg aacagtttta
     2761 ctcgttcttg ggcaaaagag ttaggaaaga ggaatgttcg tgtggtaggt gttgcaccag
     2821 ggatactgga agaaaccggt ttgagaacac aagaatatga agaagctctt tcttatacga
     2881 gaggtataag cgtcgaacag ctgagaaacg gttacagtcg aacaagtacg atccctctag
     2941 gaaggagtgg taagctgcaa gaagtggcgg atctagtctg ctattatctt tcagaaagat
     3001 caagttatat cactggagtc acgactaata tttcaggagg taaaacaaga ggataaagga
     3061 taggggggac gcacgtgacg ctagtaaaca gatggtacca attattgaaa ttactagtca
     3121 ctcacaagaa agtttcaatc caggaagtgc aaaaagaaat caaaaccagt aatcaaacca
     3181 taaaaaaaga tattggggag ttgaatgaag aaattgccgg tattgcaaaa atcatccaaa
     3241 aaaacaaaca ttttgaactt gaaatttatg attttgattt ttttgatgag atcatgcaag
     3301 gaaaattgaa aacagccagt gatttcaatt cggcatccaa aagaatcgct tatttactaa
     3361 ataggctgat cgaaacagag gatttcttac gtatggatga tttgtctgaa gaagtcggtg
     3421 tcagcagagg aacagtggtc aaggatctaa agacattaaa agagatgatc aatgattttg
     3481 atgtaaagat tacaggcaca ccaaataaag gactaagaat aataggagat gagctagaat
     3541 tacgactact tttattttat tttgtttctc cctacttttc agagacgaaa acagaacagt
     3601 ttctagaaga aaaacaagca atcaaacatt ttcagaagaa gacgaatgct agtaaacaag
     3661 aaatcaccct tttaacgaaa gttatcggta tttctttgaa tagaatctct agtaactatc
     3721 tgttgaagaa gcccatcaat cgttacagtg ggtgttttga atatatccaa gaattcaatg
     3781 aactgatcaa tcagttggaa gaaatctatg aactaacttt gagccaatat gaaaaagatt
     3841 ttatcagttt tcctttgaac atcatcaaca caagtataac gatgataagg aaagctaact
     3901 ctgatttgcg cttatatttc gaccccatga tgatgcgtat tcgacgatta gatctcattg
     3961 atctggatga gtccttctta tatcaagaga tgaaacagca cctctcttta ttgataaatc
     4021 gggtaatttt tcgttatcga ttgactgatt tattttatgg tgagatagaa aaaaagtatc
     4081 ctttttctta ccaaatcgct agcgaatgca tacaagcatt agaagctatc ttgcaacata
     4141 agatctctaa agtagagatc agctacctaa cgttgtattt ggagttagca tttcgatatc
     4201 aaaaagagca tttttcctct aaaaaagtag ccattgttca tacgacagga aaaggaacag
     4261 cattaatgat ccaaagacaa atcaaaagga tattggggag ggatatacaa ataacacatg
     4321 tttcagaaga acaatatgaa aaagtagatc taaacaaata tttcgctgtt tttacaacga
     4381 ttccgttaaa aaatgtaaat gaagagacac cggtgatcca tttgactaat ctttttgatg
     4441 actcttggtt aagggcacaa tggcaaaaag caaaaaaaaa gcgattagcc gggtttcatt
     4501 ctactgtttt tttctatcaa atatggaaag aaaaaaagac gtatcaagaa tatttaaaca
     4561 aattagtcgc tcctttgatc agaggctatt atgtggatga agaatttgaa aaaaggatct
     4621 tgaaaagaga atatcagcaa agcacatttg ttaacaaaga atttgctttt cctcacacaa
     4681 ccaaccagct atctcaacac attgttttat cagtagggct aataactgat caaggtttag
     4741 cagaaacagg aggaaatgga gttatttttt tatttgccat acctgaagaa atgacggctg
     4801 aagttgagaa tgaattattt gagctatacg atatcctatt tgaactcata aaaatgggaa
     4861 tagatgagtt tattgaatta gcaccgacac aagaatcatt tttaaaacta atcaaaagta
     4921 aaggagagag acttcatgaa tgatatgtat ctattcatag ttttcgcagc ttcagcttat
     4981 gtgctgcaag cagttatcag ttggaagcaa ctaaagcatt tcaatcaaac atatagcgaa
     5041 ctgcgaagaa aaggaaaagt agcgattggt aaacgacctg ggaaaatccg ttcaggcacg
     5101 attgttatgc tagctataga cgatcatgat caagttttag aagtcagaaa aatgcaaggg
     5161 gttacatctt tggcaaaatt tcattcatta cctgtctatg cgggtgaaga tatccattac
     5221 atcgatcgtt accatccctc agttcgagcg gaagatcgtt tgacacaagc agctttagaa
     5281 aatgccagag aagtatactt acgaatagag gcaggaaatg aaaaaagtga acaaccttca
     5341 ttgcctttta ttgctatgct caataatttt cggatatgga aaagtacgat caaagaaaaa
     5401 aggagtgaca aacgatggat atcataacaa aatttgctga gggatttatg aggctgttcc
     5461 aaacaggtgc agaaacattc ataagctgga tgacaggaat cgtaccagtt gtcttgatgc
     5521 tgatggttgc aatgaatacg ctgattgctt ttttaggtga agaccgagta acgaaagtag
     5581 ccaaattatc agcgaaaaat ccgcttactc gttaccttgt gttaccgttc ttatctgctt
     5641 ttatgttggg aaatcctatg tcatttacaa tggctcgttt ccttccagaa tactacaaac
     5701 caagctatta tgctgctcaa gctcaatttt gtcatacgag caatggtgta ttcccccata
     5761 ttaatcctgg agaattgttt gtatggatgg ggattgctca agggatcgaa accttagggc
     5821 taaattcgat ggagctggct atccgatact tgcttgtggg actgctgatg aactttattg
     5881 gtggttggat aactgatttt actactggat ttgtatgcag acagcaagga atcgttctta
     5941 gcaaaaaagt agagctttca gttgattaga gaaaggagag ttttaaatgt atggtgatat
     6001 taagatcgta agaggaaacg gcgggtatgg aggtcctttg attattaggc cgacaaaaga
     6061 taaacacaag ttcatctatg tcacaggtgg aggtgagagg ccaatgatcg tagataaaat
     6121 agaagaactg acaggtatgg agtctatcaa tgggttcaaa acatcaatac ctgatagtga
     6181 aatcgcgctt gcaatcgttg actgtggagg gactcttaga tgtggaattt atcctaaaaa
     6241 agggatacca accatcaata tcgttcccac aggaaaaagt ggcccattag cacaatatat
     6301 cactgaagat atttatgttt cagcagtagg gttagaacaa atacagccac taatggaaac
     6361 tgatgataac aaagaaacag taaatactgc atctccagat aaaaagtaca aatatgatac
     6421 ttctaaaaag ataaccgagc aaaaagcaga gcagcaaaac tggatagcaa aaattggaat
     6481 gggcgcggga aaagtagttg ctacattcaa tcaatcagca agagaagcag tagatacgat
     6541 gatccggaca gtgatccctt ttatggcttt cgtttccctt ttgattggtg tgatacaagg
     6601 ttctgggatc ggtaatgtat ttgctaacat tatggcacct ttagcaggaa atgcagttgg
     6661 attaacaatc attggcttta tttgttcact gccattttta tctcctttgc ttggtccagg
     6721 ggcagttatt agccaagtga ttggaacatt gatcggtgta gagattggga aaggaaatat
     6781 tcctccccaa ttagcattac cggcactatt tgctatcaat actcaaaatg catgtgattt
     6841 tattccagtc ggtctaggtt tggaagaagc aaaagctgag acagtagaag taggtgtacc
     6901 atcagttctt tattcaagat ttcttaacgg catcccacga gttatcattg cttgggtagc
     6961 aagttttgga ttatatagct g
//
LOCUS       DAYYCY010000129         6976 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000129 DAYYCY010000000
VERSION     DAYYCY010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6976
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.129"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            24..1178
                     /locus_tag="ACHAZB_002169"
     CDS             24..1178
                     /locus_tag="ACHAZB_002169"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HFM8614805.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            complement(1200..1952)
                     /locus_tag="ACHAZB_002170"
     CDS             complement(1200..1952)
                     /locus_tag="ACHAZB_002170"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8614806.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(2316..4982)
                     /locus_tag="ACHAZB_002171"
     CDS             complement(2316..4982)
                     /locus_tag="ACHAZB_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFM8614807.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            5323..6774
                     /gene="cls"
                     /locus_tag="ACHAZB_002172"
     CDS             5323..6774
                     /gene="cls"
                     /locus_tag="ACHAZB_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFM8614808.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
BASE COUNT         2054 a         1447 c         1273 g         2202 t
ORIGIN      
        1 atcataagta aagaggtgtt gaaatgaaaa tagggattga tcgtctttcc ttttttattc
       61 ctaatttata tttagatatg acagagttgg cagaaagccg tggggatgat cctgcaaaat
      121 accatatagg gattggtcaa gaccaaatgg cagtcaatcg tgcaaatgaa gacatcatta
      181 cactaggagc aaatgctgcc agcaaaatcg taacagaaaa agaccgtgag ctaatcgaca
      241 tggtcatcgt cggaacagaa tccgggattg atcattcaaa agcgagtgcg gtaattatcc
      301 accatttact gaaaatccaa tcttttgctc gttcttttga agtaaaagag gcttgctacg
      361 gtggcaccgc agctttgcac atggcgaaag aatatgtcaa aaatcatcca gaacgaaaag
      421 tactagtcat agcaagtgat attgctcgtt acggcttggc aagcggtggt gaagtgacgc
      481 aaggtgtcgg tgctgttgcg atgatgatca ctcaaaaccc gcgtatttta tcgattgaag
      541 acgacagcgt atttctgaca gaagatatct atgatttctg gcgtccagat tatagcgaat
      601 ttcctgttgt tgatggtcct ttatctaatt ctacgtatat cgaatcattc caaaaagttt
      661 ggaatcgaca taaagaattg tcgggtcgag gactcgaaga ttatcaggcg attgctttcc
      721 atattccgta tactaagatg ggaaaaaagg cattgcaaag cgtattagac caaacagacg
      781 aagaaaatca ggaacgtctt atggctcgct atgaagaaag catccgttac agccgacgaa
      841 tcggtaatct ttacactggt tcattatacc tggggctaac ttctctactg gaaaattcga
      901 aatcactaca gccaggagat cgcatcggtc tattcagcta cggttctggt gcagtaagtg
      961 aattttttac aggttatctg gaagaaaact atcaagaata tctcttcgca caatctcatc
     1021 aagagatgct agattctcga acacggatca ctgttgacga atatgaaacg attttcagtg
     1081 agacgcttcc tgaacacgga gaatgtgcag aatatacttc agatgtacca ttttcgatta
     1141 caaagatcga aaatgatatt cgttactaca aaatataata aaatcataaa aagaaccctt
     1201 cacgagtcgt gaaaggttct ttttttatct gcggaccaaa ggagcagtct ataggtcagc
     1261 ttagtcgtac taaggatttc tcgtttttca ctgaatagtt tgcgaatgta cctggtcaca
     1321 acaggatctt ttgcgtacgc gtcatagtat agcttcatca attcttcagt aattgattcc
     1381 tgtgtaaaat aagtaaaatc ttttttcttc actttgaatc cttcatcaat taagtatgtt
     1441 tcaaattgat ccattacaac tggcgatgta ttaggatctt cttctatcat tcctagttgc
     1501 ttttcgatag gataaaaaac atcatcgatt gattccacta ctctgattat cacgcaccat
     1561 ttgcttgact ttttcatcaa tttttctaat tgaataagat gtaccaatgc tggatttgct
     1621 gctgtaaaaa ccatttcata tgtctcattt gattggataa attcttctaa cgactgtttg
     1681 ataaaggaaa catttggcaa ctcatttttt tctgcttctt tttttgcatt ttctagcatt
     1741 cctgaagaaa aatcgatcaa atcatagcga ttaatttctt ttgctaagga aagaaatctg
     1801 ccagatcctc cagcaacgtc tgctgctgtt tcagcaggca ggactttctc cgttttcaga
     1861 taatttttta catcttcagc aatagttact tgcgattctt gctggatttc ataatattct
     1921 tcagcaaatt catcccattc tttttcattc atttgatttc ctctaatttt ttgttatatg
     1981 ttttcacttt tcggaataaa tcattctcct cttttatcta ttctatctct tttgatgaat
     2041 aataaaatga ttggctttcc gtctatcata aataaaaaaa gggactgtga ccaaagtcgt
     2101 gtcaaagtcc ctctttccga ataaacagtg tccaatcaga tgaccctcag tattagctca
     2161 aaatgctgat tttacaactt cttccttaaa cggtgttctc ctgccaactc ttcggtcaca
     2221 agtcacaata ttcaacaaac atgagaagct gtttgttgaa attgcacctt gtgactcaga
     2281 attaaacggc aggctcacaa cctcgttgat tcgttttatt tatttccttg tactcgtaca
     2341 atgatcaatt tagtgatttc catcaaaaca attgtcacaa gtgcgattcc catacatgta
     2401 aagacttctg tcaatccaaa tgctgatggg atagagaaga cttctttcag tcctgggatc
     2461 attgttaagc agtaaagcgc gctacataca actacagcta gtaagacact tctgttagag
     2521 aagaaccctg cttggatagc tgtttgtgtg tttgaacgtg ctgggaatgt ttgcaatgtc
     2581 cgacaccaaa tcaatgttga aaatgacatg gctacaccga attcaggtga atgctgcatg
     2641 ccaagccatt gagcaaagat aacagcaatc ccaatcagca caccacgata cgttacagaa
     2701 atcaatgttt tgtccgtaaa gattcctgaa tttggatctc ttggcgggcg gttcatcgta
     2761 gcaggttcag ctttttctgt acctaatgca attgctggca gtgaatcatt gactaaattg
     2821 atgaacaaca gttgtaatgc tgtaaatgga ttggaccagt cagcaactaa ggcaaagatg
     2881 atcgcaatga tcgcacctaa atttcctgca aatagataag aaatagcttt cttgatatta
     2941 tcaaacacat tacgtcctac ttctactgcg ctgacaatag aagcaaagtt atcatccgtc
     3001 aataccatcg ctgctgcatc ttttgccaca tctgtacccg ttcccatagc aattccgatg
     3061 tctgcttgtt tcaatgctgg tgcgtcattt acaccatcac ctgtcatagc tgagatatga
     3121 cctttatttt gccaagcacg aacgatacga attttattct caggtgaaac acgtgcataa
     3181 acagtgactt tttctaaaac ttcgtttagt tgatcttctg tcaattcatc tagttccgtc
     3241 ccggtcaaag ccaggtttcc ttcttcaaaa atcccgattt gttttgcgat agcaacagct
     3301 gttgttttgt ggtcaccagt aatcatgatt gttttgattc ctgcacttct tgcttctgtt
     3361 accgcctgag tcacttcagc acgtggagga tcgatcatag caagcaaacc aacaaggatc
     3421 aaatcgtctt catcttcaaa tgataaatca aaattatcaa gtggacggta agcaaaagct
     3481 agtacacgta atgcgcgtgt tgaaaaagct tcattttgct cttggaattt tttcatcaat
     3541 tcatccgtca tcggaacgac ttcaccattt agcaatactt ttttactgcg ttggaagacg
     3601 atatctggtc cgcctttaac aaacaagtaa tttttgttat caattgtgtg tccagtagac
     3661 attaattttc gatctgagtc aaaaggaagt tctgcttgtc ttgggtagtt gtttcggatt
     3721 tcctgataag gctggtttaa tttttcacta aagtcgataa acgctacttc cgtagggtcg
     3781 cccaatttag taccgtcttc actgatcgat gcatcattcg ctaaaacact gatttcgatc
     3841 aagcgacgtt cgtcagctgt ccattttgaa gggtcatttg tgaaatcccc agtatcgcca
     3901 ttacctaaaa agtagtcaac aactgtcatt ttgttttgtg tcaatgtacc agtcttatct
     3961 gtacagatga tacttgtcga accaagtgtt tcaactgctg gaagcttccg aataatggca
     4021 tgacgttttg ccattttatt cgttcccaaa gataacacga ttgttacgat agactgtaat
     4081 gcttctggaa tagcagctac ggctacggct acggcaaaca tcactgcgct gacgatatct
     4141 gcagtcatat ctcctgtacc ttctccaaga taaatacgaa acagttggat acctaaaatc
     4201 aataaagaca atgccaaaat agccaaactt agttttttac tgaatttatc cagacctcgt
     4261 tgtaacggag tgacttgctc agttgttgat tccaaaaggc cagcaacttg tccgatctcc
     4321 gtattattcc cagttgaagt cactaagaat ttaccgcgtc cgtaagttac gatcgttcca
     4381 ctgaatacca tatttgtacg atctccgatt ggtaccgtac ttgtgatatc cgttacttct
     4441 ttttctgcag aagtcgactc tcctgtcagc ataccttcat ctacttttaa tgatcccgca
     4501 tctagtaaac gtccatcagc aggtacataa tcgcctgcgt ctaacaacac gatatctcct
     4561 accacaagtt cgtttgccgg aatcgttttt tccttaccat cacgaatcac tcgagcatcg
     4621 ggcgctgaaa ggtttttcaa cgcttctaaa gaaccttctg ctttttttgt ttggacaaca
     4681 ctgattatag aattcaacat caatactgca aaaataacta agaattcgat gtagtcaccc
     4741 atgaccatct gaatcagtgc gacaatcagc aatacgataa ccattgcatc tttgaaagtt
     4801 tctaagaaca atttccaagt tggcttcttc ggcttttctt ttagctggtt caagccatct
     4861 ctttctaatt tttccttcgc ttgcgtggtg ctcaatccat cggttgagct ctgcgtttgc
     4921 tgcatcaacg agtcgatcgt ttctttatag accgcttgtt gtgcttgttc cttgttttcc
     4981 aaatttcatc ccccattttt tgatcttggc cctagtcctg tctgaaaaaa aagagaccta
     5041 agacaaatac gcatttgtct taggtctcac catttcatac aataccagaa aaccatttgg
     5101 ttaacttact gttgatatta tcgcaacaaa cgttgccggt tactccccta aggagatatt
     5161 caactgtact aatatcctac cttattaacc tacaaaaagc aactacttta gcccgattta
     5221 cctacatttt catgaaagga gtgaaaagta tattcacctt ggtaggcttc tttcttctat
     5281 catgctatac taatgttatt gtatgtaagg aggaacattt ttgtggtatc tagtattata
     5341 accgcccttt atctattaaa tgcacttatt gctttggtgg ctattttgat caaaccccga
     5401 gatgtagcag ccatttgggc atggctctta gtattgtttg cccttcctgg ggtgggcttt
     5461 gtattatatt tatttttcgg acgtggatta acggataaga aaaaattcta tctccgacaa
     5521 agtgacttga aagaattaga aaactttcag tcttttagag gagataccat tgaacattac
     5581 gatcctgaca tgggcgataa agacaaacag caatttgttg acttcttctc ttcattaaat
     5641 cgtatgccgc tgacaagaat gaattctgtc actcttctca cagacggaca agagaaattg
     5701 gattcactgc ttcaagatct aaaaaaagcc aaacattcga tccatatcga atattacgca
     5761 tttgtgacag ataatatcgg ccagcaagtc ttacatgttt tagaagaaaa agccgcagaa
     5821 ggcgtggaag ttcgaatatt atatgatgca tttggctctc atggcacaaa agcaaaagat
     5881 ttcaatcgtc taatcaaaaa tggtggacat gtccatacat ttgttacctc acaaagggca
     5941 ttacttcgtt tccgattgaa ttaccatgat caccgaaaaa tcgttgtgat cgatggaaag
     6001 attagttata ccggtggttt caatattgcc aatcaatatg taaatacaac aaaaaaattc
     6061 ggctattggc gcgatacgca tatacggatt ttcggtgccg cttcttcttt gctccagctt
     6121 cgcttcttaa cagactggaa cgtctcggta cctgaagaaa aaaaggtcgg ctatcatttg
     6181 aattatttct ttaaaaaagc agatcgagat gaatctaagc ttgctgatac atccatccag
     6241 cttgtttcaa gcggaccgaa taacgaaagg gaacaaatca agctttcatt tatcaaattg
     6301 attacttctg ctaaaaaacg tgtttggata cagacacctt accttgttcc tgatgaaagt
     6361 gtcattgctg ctttaaaaat cgcaactgcc tctggtgtag atgtgaaaat catgattccc
     6421 aacaaaccgg atcatccttt tatttatcga gcaacacaat attatgctcg gcagctgatc
     6481 aaggaaaatg tacaaatcct tgtctatgag aacggcttcc tccatgcaaa aacattgata
     6541 atggatgatg aaatctgcat ggtaggttca gcaaatcaag atattcgtag ctaccgattg
     6601 aattttgaaa caagtgctgt catttacgat cctgagtttt tagaagaact tgctactcag
     6661 ttcaaagaag atgagacaca ttgttcatcc atgacaactg aaacagtcaa ggaaatgtct
     6721 aactggctat tattcaagca acaaatttct cgattatttt ctccaatcct ataatttttt
     6781 tattcaagta gagaaaaata tactaagatt aaagggactg tgacaaaaat cttgtcacag
     6841 tcccttcttc cgaacaaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt
     6901 caacaaacat gagaagctgt tttttgaact tgttccttgt gactcggagc tggacggcag
     6961 gctcacgacc tctttc
//
LOCUS       DAYYCY010000130         6957 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000130 DAYYCY010000000
VERSION     DAYYCY010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6957
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.130"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            256..1770
                     /locus_tag="ACHAZB_002173"
     CDS             256..1770
                     /locus_tag="ACHAZB_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HFM8614809.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(2173..3156)
                     /locus_tag="ACHAZB_002174"
     CDS             complement(2173..3156)
                     /locus_tag="ACHAZB_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HFM8614810.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(3161..3808)
                     /locus_tag="ACHAZB_002175"
     CDS             complement(3161..3808)
                     /locus_tag="ACHAZB_002175"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding oxidoreductase"
                     /protein_id="HFM8614811.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(3893..4525)
                     /locus_tag="ACHAZB_002176"
     CDS             complement(3893..4525)
                     /locus_tag="ACHAZB_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFM8614812.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(4541..5092)
                     /gene="coaC"
                     /locus_tag="ACHAZB_002177"
     CDS             complement(4541..5092)
                     /gene="coaC"
                     /locus_tag="ACHAZB_002177"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HFM8614813.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(5089..5826)
                     /locus_tag="ACHAZB_002178"
     CDS             complement(5089..5826)
                     /locus_tag="ACHAZB_002178"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HFM8614814.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(5823..6812)
                     /locus_tag="ACHAZB_002179"
     CDS             complement(5823..6812)
                     /locus_tag="ACHAZB_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFM8614815.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
BASE COUNT         2127 a         1344 c         1439 g         2047 t
ORIGIN      
        1 tccagacctg atccactagc ataataacga cgatgatacg taataaaata tagattccgt
       61 ttgtgattct acctgcagtt tttgtactgt tcgtaaaaaa ggttttcaaa tgattcataa
      121 aataactcct tcatgtatat ccttaattat actaagaagc gtgtgtttac caaagctata
      181 tgacttaaag aaaaacagag ttgatattgt gaaaaaaatc acttaatata gtataaggag
      241 aaaaggggga tacaaatgaa aaaattagtt gctggtttat taacagccgt tgcacttctt
      301 gctatgagcg catgcggcgc agatcagaaa aatacagcgc agacaagttc tagtacgaag
      361 gaatctgccg aagtatcatc aggcgcttct gaacaagtat atacagatcc aaaagaaatg
      421 aaagaatctt atgatgtcgt gattattgga gctggtggtg cagggatggc agcagctatc
      481 gaagcaaaag atgctgggtt gaatccagta attttggaaa agatgcctgt tgccggagga
      541 aacacatcta aatcatcaag cggtatgaat gcttcagaaa caaaatttca aaaagaagaa
      601 ggaatcaaag actcgaatga tgccttctat gaagaaacgc taaaaggtgg tggaggaacg
      661 aatgataaag aaatgcttcg atactttgtc gatcactctg ctgatgcggt cgattggcta
      721 gatcaaaacg gtatcaaact ggacaactta acgatcactg gagggatgag tgaaaagagg
      781 acacatcgtc cgtctgatgg ctcggctatt ggtggatact tagtggaggg gttattgaat
      841 aacgtccatg agcgggaaat ccctattttt gtcaacgcag atgtgacaga cattaagaaa
      901 aatggggaaa cggtcaatga agtgactgta catgttcaag gagaagaacc aaaagaggtc
      961 accggaaaag caatcgtagt taccacaggc ggttttggtg ccagcaaaga atttattgaa
     1021 aaatatcgtc ccgatttagc tgattatatt acgacaaatc aagaaggatc aactggagat
     1081 ggtatcacca tgattgaaaa attaggtgga caaaccgttg atatggataa aatacaaatt
     1141 catccaactg tgcagcagga tgagggggtc ttgattggtg aggttgttcg cggagaagga
     1201 gcaatcttgg tcgatcaaga ggggaaaaga tttgtcaatg aaatggatac aagggataaa
     1261 gtatctgcag caattacagc attgcctgaa aaatcagctt atttgatttt tgaccaaggc
     1321 gtgcgtgatc gtgccacagc tattgaattt tatgaccaaa aaggatatgt cacaactgga
     1381 aagacaatgg aagaattagc agaaaaaatc gatttaccaa aagaaacact agcaaaaaca
     1441 gttgatggat ggaatacgga tgttgcggag aaaaaagatg ctcaattcaa ccgtgccaca
     1501 gccatggaac atgacttatc gtctccgaat tattatgcga tcaaaatcgc accaggaatc
     1561 cattacacga tgggcggtgt gaaaatcaat acgaacactg aagtattaga taaagaaaac
     1621 cagccaatta gggggttata tgctgctggt gaagtcacag gtggcttaca tggaaacaac
     1681 cggatcggtg gaaattcagt tgcggagatc attatttttg ggcgacaagc gggcatacaa
     1741 gcagctcgat ttgcttcgga aaatgaatga aatagaacgt aaataaaaag aggttgtgaa
     1801 atcagcgttt tgagctaata ccgagggtca gctgattgaa cactgtttag tgaagaggtt
     1861 gtgaaaaagc tgccctgcat caagtaataa gaacagccaa gtaaaaacag cttcttatgt
     1921 ttttaacttg gttggactta ttaccgcaga tgcaagcttt tgaacaccgt ttattcggaa
     1981 aaagagcgtg ggacaaaagt aaagaacact tttgtctcac gctctaaaca cgtataaacg
     2041 gcggaagcag aagaaattcc ttcggaaata agctgaaatt cacaaaaatt tgaaaagcaa
     2101 ttttcgtgaa tttcttctta tttcttggag ttaaacgctt ctgtcccagc ctcttttttg
     2161 atcagcatga ttttattcag ttatcttcaa aatgattttt ccgatgtttt tgtttgcttc
     2221 catatattca tgcgcttgtt tgactgcgct gaattggaat gtacggtcaa tgattggccg
     2281 aatcttgcca ttttctagaa aaggcccagc tttttgcata aaagtttgtg cgagctctgc
     2341 tttatactga tcacttctag gtgtcaataa tgtaccaagc agttggacac gtttcccaat
     2401 caatacacca aggtctatct caggcacgat ggtcccacct agcataccaa tcaagaccca
     2461 acgtccatca acggctatag aagctaaatt cttttcccag taagaagcgc cgatgaaatc
     2521 caaaataaca tcgactcccc gaccttcagt gatttttcgt acctcttctt gaaagtcttg
     2581 ttttttatag ttgatcaatt catctgcacc aagttcttta caaaaggcga gtttttcttc
     2641 tgatcctgca gtaacgatga ttttcgcgtg agaaagctgt ctagctaatt ggatcgctgc
     2701 tgttcccaca ccacttgctc ctgcatggat caagacagtt tcgccttctt gtaattttcc
     2761 aagccagtaa agtgtttgat aagcagtcaa aaaaacttct gagattccag cagcagaaac
     2821 atagtctagc gtatcaggga gaaggattgc acgatcggca ggcatcacag catattcggc
     2881 ataaccacct agattgacta atccgtatac ttttgtccca ggagtaaaag tggagatatc
     2941 acttttattc tcaactacct ctccagctac ttcgactccc aaaatagggt agggaggttt
     3001 caaagagact gattctcttt tcataatatc tgttcgattg acagctgctg tatggacttt
     3061 gatcagtaat tcgccttgtt ttggtttagg aacaggcact tcaatttgtt ctaatacatc
     3121 tacagaacca ggatgcttga ttgaaatggc acgcatggat ctattcctcc tttgcccatt
     3181 cttggaatgc tgtattcaac tctttgctac cttcagaaag tactagagtc gtacgagaaa
     3241 tatccgtttc ttccactaga gcggcaagta cagcagcaac tgtagagcga gtgatcgttt
     3301 ttgatgcctt ctcattaggc ttagttaatt gaatggaagt aacttcttca tcgttagtca
     3361 atgtgactgg ttgaagaatg acaaaatcta agttagttcg tttcagccat tcatctgcat
     3421 aatgtttagt aatataataa tctgtcatgg actcaggcca ttttgctggt tcatctgcat
     3481 aaacagcact gaccatcaga tagcgagaga catgcgcatt ttcagctgcg atcactgttt
     3541 tgattgcgcc atccaagtct acttgcagaa gattctttcc ttgagagccg gcagcaaaaa
     3601 tcaatacatc gatgcctttg aatgcttcgg tcattttttc cacggaccaa gttaaatcaa
     3661 aagagacata cgaaacattc tcttttttta ctaatgtttg ggaagcaaca tctctcaccc
     3721 ctgcaaaaat ttcatgggaa cttggagcta aatcttttat caggtgtcgg ccaatttgtc
     3781 cgtttgcacc tacaacaaaa attttcattt ggatcatcct ttcgacgtca aatctttcca
     3841 ttcaatagta acaccaaaag agaaaaaaat cagagatcgg ctacgatgac tatcagttag
     3901 taagagaagg gtgcttgaag tatcttaaca agacattgca tactgctgca gtcgcaagac
     3961 ctgcgacgat tccttcaggt ataccacttg taaaaataac cgctagaata gctgaataaa
     4021 cagcatttaa atttgttccg atgactttgg cataatcttg ctggaataaa aagtagatca
     4081 tattcatcac taaaatcgta ttggtcaatg aaccggaaag tccagctaag aataaagaaa
     4141 cggaatctgc tttttgtttc attagtttgc gcagaccttt ataaacgaaa taaggaacca
     4201 ccccaatcaa gatgcgagga ataaacgcga tcagcaaggc tttccagctt ccatgttctg
     4261 ttccgatgac tgggataaac ggagaaaaga caaaagacaa aggtgtttgg ataactgtgc
     4321 tacggatcat gctgatcagt ccaaatgtac cgccaagaaa agcacctagt cttggtccta
     4381 aaacgatcga agcaatgatt acaggaatat gcatggttgt tgcattgatt ggaccgatag
     4441 ggataaaacc taaaggcgta acagctaata aaatcaaaat agctaaaaac atcgcagtta
     4501 atgtaaagtt ttttgtattt ttcataagta ctcctttttt ctaagcattt ctttcattaa
     4561 gagtcagcat gacctgttcg atgatcgtct gaatatcagc taaggcacct tttccaaaat
     4621 ctccacaagc taaaagagat tctcttggtt caatttcctg atagccgaca gatctcaatg
     4681 tttccagatt acgctgagtg atcgggtttt gatacatatt cgtgttcatg gcaggggcaa
     4741 tcaattttgg tgttcccact ggaagcgcca ttgcgactgt tgaaagaaga tcgtctgcaa
     4801 tgccgtttgc tagcttgccg ataatgtttg ctgtagcagg cgcgatgaga aacaagtcag
     4861 cttgttttgc tagttcgata tgattgatta cctgcggatt tgattcttgc atgacgtctg
     4921 tatggactgc gcgcttcgac agagattgca aggttaatgg cgtgataaaa tgtgtgctgc
     4981 ttttagacat aatcacatct acctgggcac ctaatttgac gaattgactt gtaagatcag
     5041 cgcttttgta agcagaaata cttccagtaa cgcctagtaa gatctttttc attttgattc
     5101 ctccagtcta attttctcag taatcaatgc agcaatctca gatttacttt gagcttcttc
     5161 cactgttcca tctttagata agagatagcc gtggtgtgtc tcgccatgga tttccgtcaa
     5221 atcatttgcg aaaataaaat cagttttgtt atttatcaga ctattttttg cgactgccag
     5281 aagagattcc tttggtacat ctactaatag tttgaaacca atgatttttg cttttggctg
     5341 ccattcacgt agataagaaa tgatttttgg tgttttcttc aaaacgagca caagatgctc
     5401 ggtgttagaa gagatctttg aaacagtggt atctgtctgt tcattttccg caaaccaatt
     5461 gtccagttct tcttgcgttg aagaagtggg taggtttttt gccagctgtt cttctgaaaa
     5521 actaaaagca ggagtaaaat cactgactgc catactgtgg ataatcgcat cgtagtgttc
     5581 ttttttcgtt agttcttcta attgtaaaaa taagtccagt gcagattcga tcgtataaat
     5641 cgttatattg gagtgtcttt tcggtttcag tgcttgtttc gtagtcacgt aatcgattgt
     5701 ggtatccgga ttggcagcta aataatcagc taatgcttgc cctaatcgtc ctgtcgaatg
     5761 atttgtaata gcacgtactt gatcgatttt ttcagtcgtt cctccagctg tgattagaat
     5821 cttcatttat ggtcacctac atttactagt gttcttcctt ggtgtgttcc agcaagtagt
     5881 ttagatgctg tttcaggcaa ttgatccaga gtaatctctt ggacgaccag ttcatctgca
     5941 atctgcaaat ccgccatctg ttgccataaa gaaagacgtt gatctattgg tacattgaca
     6001 gaatcaattc caatcaactg gatgtttcgc agaataaaag gcaagacagt cgcgtttagt
     6061 ttgattcccc cagcattgcc acataaagct actgcaccgt tataagagat caatggaaga
     6121 agactgctta gctgctcgcc acctactgtg tcgatgacat agtcgatttg ctgttttcct
     6181 aaagcttttg ttgtctctgg cagaaattca tcaggtcgta cgacttgcaa agcacctaga
     6241 ctttttagcc atgtgacaga ctcttctttt ctggaaaagg caatgattga ctggaatcct
     6301 agtttatgta acagggctat cgctgtactg cctacaccgc cagaagcacc agtaacgaca
     6361 acttttttat cggaggtcat accttgacgg attaaagcgt tcacacacaa catagcagta
     6421 aaccctgctg taccaaggat catcgcttga cgcagcgtca tattttttgg tagaggaact
     6481 aaccagtctc ctggtacttt ttgatattgg ctataaccgc ctggatgggt gacgccaagc
     6541 ccaaaaccag tagcaagtac aagatcttct tttgaaaaac gtggattgtt actttggacg
     6601 acagttccgg ataaatcaat ccctggagtc atcggatatg ttcgtatcac ctgaccagac
     6661 tcgctcattg ccaatgcatc tttataattg acatcagaat aggcaatttt taccaagact
     6721 tcattttcag ccaactcagt aaatggcaga tcactgattg ctgtatggaa aacaggttcc
     6781 tttattacct gaaaagatcg aaaagcgttc attcgtgaga cactcctttt aaaattttct
     6841 tttatcatac cataaagcct actcttttat tcttgttctt ccgtaaattt agacaaaaat
     6901 taggtgaaaa ataaagaatc aaacaactag aatttattcc aaaagataaa gaaatat
//
LOCUS       DAYYCY010000131         6883 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000131 DAYYCY010000000
VERSION     DAYYCY010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6883
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.131"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(57..1586)
                     /gene="ahpF"
                     /locus_tag="ACHAZB_002180"
     CDS             complement(57..1586)
                     /gene="ahpF"
                     /locus_tag="ACHAZB_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFM8614816.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(1596..2159)
                     /gene="ahpC"
                     /locus_tag="ACHAZB_002181"
     CDS             complement(1596..2159)
                     /gene="ahpC"
                     /locus_tag="ACHAZB_002181"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFM8614817.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(2303..2965)
                     /locus_tag="ACHAZB_002182"
     CDS             complement(2303..2965)
                     /locus_tag="ACHAZB_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8614818.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            3149..4795
                     /locus_tag="ACHAZB_002183"
     CDS             3149..4795
                     /locus_tag="ACHAZB_002183"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFM8614819.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            4786..5868
                     /gene="uxuA"
                     /locus_tag="ACHAZB_002184"
     CDS             4786..5868
                     /gene="uxuA"
                     /locus_tag="ACHAZB_002184"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFM8614820.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(5976..>6883)
                     /locus_tag="ACHAZB_002185"
     CDS             complement(5976..>6883)
                     /locus_tag="ACHAZB_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HFM8614821.1"
                     /translation="KLYFLLKICKENFPATSVKDVQKEFTEYEYIDFFIDLSVDILRP
                     NLPICREQRILVNILARLSITKIESEETSLEQYKLLKKANVPHVLYSREMVESLCTVF
                     DLFIPEKEKILYSLAFARNKLYNGFLNFNQPNTPLPTFMLYQEHSVLKEVQTAIEQFY
                     MDFREQNQHLLPVILEKENVQWMVEDLVHLYDRYKKQPRIVIGVNYTRDFYISKDLIV
                     KIEQIFSNESIKIQNDFMENCNIVISDCPLKQLPSHIKKIYILKGIITPEDWKKVIMR
                     ISEYIFELKEEALMDQSKKENQVTS"
BASE COUNT         2140 a         1274 c         1288 g         2181 t
ORIGIN      
        1 aaaattcttg tcaccatcct tttttccgct ctctgacttt ttacaatctt catagctcaa
       61 gtgcgcatca gataatcgaa tgctcctaaa gctgcagtag ctcctgaacc cattgaaaca
      121 atgatttgtt tgtatggact atctgtacaa tcaccagctg cgaaaacgcc ggggatattc
      181 gttgcgccat ttttctcggt cactatttca tttcttggat tcatttcgat cgttccatct
      241 agccaatccg tattagggag taagccgact agaatgaata ctcctgaaac agctagtgta
      301 tgcatctcca tcgtacggcg atcgatatag gagagagatt ctacctgatt cgtaccgctg
      361 atttctttcg tcgctgcatg tttgataaca gtgacattcg caagagagac tagtttatcc
      421 tgtaagattt gatctgcttt taattctggt aagaattcta gaacatatac atgttttgct
      481 aacccagcca aatcgattgc tgcttcaaca ccagaattac caccaccgat aacagcaatc
      541 tcttttcctt taaataaagg gccgtcacag tgtgggcaat aagcaattcc tttattttta
      601 aactcttttt cgcctggtac attgatagaa cgccagtgcg cacctgtcgc gatgatggcg
      661 gtttttgctt taagtgatat accgttagct aagccaatat taataaaatg ttgctcttta
      721 tttaaggaaa cggcctgttg attcatcata atgtcgatag ggtaactacg cacatgactt
      781 tctacttgct gcatcaattt tggaccttct gtgtaagagg taccaatcat attttcaatc
      841 cctagtgttt caacgacttg tccgccgaaa gaatcagcga caagtcctgt acggattcct
      901 ttacgtgctg catagatagc tgcactacta gctgctggtc caccgccaat cactaataca
      961 tcataaggtt ctttttcaaa caactcttct tcaatcaaag ggccagtgat tttttcaaca
     1021 atttgttcga tcgtcatgcg tccgctacta aattcttctc catttaaaaa cacagttggc
     1081 actgccatga tttttttatc atcgatttct ttttggaaca tcccaccttc aatcatcgta
     1141 tgagtaatat ttggatttaa tacagctaat atattcaatg cctgtacaac gtcggggcaa
     1201 ttatgacaag tcaagctaac ataactgtca aaatgcaatg tttgtgttat ttttttgatt
     1261 ttaagactca aagattcact gattttcggt gcccgtccac taatttgtaa catagccagt
     1321 aaaaaagagg aaaattcatg accgaggggg ataccagcaa aaacgattcc actttctttg
     1381 ccaactggat tgatagaaaa actaggtgtg cgcatcgaat ctcttttttc ccaagagatt
     1441 ttaggagaca tttctgctat ttccttcgta aattcagcta gtttttgact attttcacta
     1501 ttatccaaac taacagaaaa aacaatagga gattcaatca gtgctaaata ttgttcgaga
     1561 ttttgtttag tttctttgtc taacatatcc accacctaaa tctttccaac aagatcaaaa
     1621 cttggtttca atgtttcgcc actttctttc cattttgccg gacaaacttc acctgggtgg
     1681 gtacgtacat attgagcagc gcgaatctta tcaatcagtg cactagcgtc acgaccgatt
     1741 ccatctgcat tgatttccat agcttgaacg attccgtctg gatcaataat aaatgttcct
     1801 cgctgagcta atccttgctc ttcatccaaa acatcaaaaa tacgagaaat ctgatgagac
     1861 ggatcaccga tcatcacgta ttcgatttta ccgatagcat ctgatgtatc atgccatgct
     1921 ttgtgagtaa agtgggtatc ggtagagcaa gaaaagactt caacacctaa agatttcaat
     1981 gtactgtatt gttcttgtaa gtcttctaat tcagtaggac aaacaaaagt aaaatcagct
     2041 ggatagaagc aaagaatact ccagttcccc ataagatctt tttcactgac ttctaaaaat
     2101 tctccttgat gataagcatg tgcattaaat tcttcgattt ttttcccaat caatgacata
     2161 gtaaaacctc caataataat tatttattta taattattgt aatttagttg gagttgcaaa
     2221 gtcaatggat attttgttat ttaataaaga ttactcaaaa acgaaggtta attataaaaa
     2281 tagaagaggt cgcaagaaaa tgctactgct ggaaatactc gggataacgt tgagtaacag
     2341 tttcctgttc tttcagcatc atgtgtaagt gcaatcccgt ttgaaatcga gcttcttctg
     2401 gttttttatc aactaaagcc ttgagtaaat cataatgttg ttccatgatc gtttcccatg
     2461 gaagcccctg aatggtcact cgtaaccagc ggaaacgttc taaatgggta ttatggtcat
     2521 ctaaccagtt ccaaacttct tttcttcctg caatttcgaa acaagcttca tgaaataaat
     2581 tatctgcacg gaaaaaagcg cgttcgtcat ttattttgac tgctttttct tgttcttcta
     2641 aaatcgtttc tagcactatt cgtgtttgtg tgtccaattt ggaacaacat tcaatcatga
     2701 tttgctcttc caactgttct cggacaaagc gagcattttc tgcagaaaca agattaattt
     2761 tggaaacata cgtaccactt tgaggtatcg tatacacgag ttcttgctga cgtagttgaa
     2821 tcaatgcttc gcgaatggga gtccggccga tagcaagatg ttcctccagt gccttttcag
     2881 atattttcct tccaggttct aagtcagcat agataatttg tttacgaatc gcttgataag
     2941 cttgtgattg caagctgctt ttcatcttta ttcctccaaa atcatttcta tgaacgtagt
     3001 ataacagaag aggtctgaaa aacaatctgg tatgtcacat taactagtaa atttgtataa
     3061 atagtaaaat tttggttgac atagcgattt ttattgcata aaattcactt gaatacaaaa
     3121 actaatatgt tagtttagga ggggaaaaat gattgattta aacaactgga ggaactttat
     3181 ccctgaatta tctcaaaaag aagtagaaat acctcatttt gaccgtgcat cattaagaga
     3241 aaatggacaa aaaaatcctg tctggatcca tttcggtggc ggaaatcttt atcgaggatt
     3301 tcatgcagaa atcgctcaac gtttagcgga tctaggcgaa ttgtccactg gtgtcattgt
     3361 ttgtgaaaca ttcgatgaag aaataattga aagggcttac caagattttg atgatttgat
     3421 tcttgaagtg gtcatgccaa gcagtggaga gttcgtcaaa cgtatattag ctgcgacagc
     3481 tgacagtctg tattgtcatc cagattgttc tgaacacttt gataagatga ttcgacactt
     3541 tgaaaatcct agtttacaat tagttacttt tgctattact gaaaaaggtt atcgattgaa
     3601 agatacaaat aataaatata taggaccgat tgcagtcgat ataaaaaatg gtccaaagaa
     3661 gccatcacat acaatgagta ttcttgctgt gctactttat aaacgttttt tatctggagc
     3721 ttggccgatt gcacttgtct ctacagataa tttttcacaa aacggtagtc aattacgtac
     3781 ttctttgcta acgatcgcaa aagaatggca aaaggaaggt tatgtacaaa atacatttat
     3841 ccaatactta tcgaatgaag aaattgttgc gtttccatgg acgatgattg atcgtatcac
     3901 cccgaatcct tccttgtcag ttagcaaaat gttggcaaaa gagggctttt caaatatgtc
     3961 gattttccaa accgcaaaag gaaataatat tgctccattt gtcaatacag aggacatcca
     4021 ttatttaatc atggaagatc attttcccaa tggacgtccg aaattcgaaa aagctggggt
     4081 attcttgact gatcgtgcaa cggtagataa agcagatacg atgaaggtga cgacttgcct
     4141 taatcctttg catacgtctt tagctattat gggttgttta ctggggtata ggagcatagc
     4201 tgaggagatg aaagatccag atttatcgct gctagtttct gaaatcgggt acaatgaagg
     4261 cctcccagtt gtggagtctc caggtatcat tgatccaaag atctttctgg aagaagtaat
     4321 tactgtgcgc ttttctaatc caaatattcc taatatgcct cagcgaatcg ccacggatac
     4381 ctctcaaaag gtggcgattc gttatggtga gacgattaaa aaatatatgg aaatagaagt
     4441 taaagacccg cagacacttg tattcatacc attgactctc gctgcttggc ttcgatacct
     4501 tattggcatt gatgatcaag gaaaaccatt catcccgagt ccagacccac tattgacaga
     4561 ccttcagcat aagctttccg atattcaact gggtacgcaa gatacatcac atattcatga
     4621 gaaaatggct cctattttgt caaataaaga aatttttggt gtagatcttt accaagcaga
     4681 gcttggcaga aaaatagaag aaatgttcat caagatggta gcaggaccaa atgccgtacg
     4741 aaaaacatta caggattact tacaagaaca tggaggaaat ttatcatgga aatgactttt
     4801 agatggtacg gatcacagga agaaaagatt accttagatg aaatcagaca gattcctgcg
     4861 gtcaaaggga ttgtaggcac attgatggat ataccagtag gagaggtatg gcctagggag
     4921 aggatcaaag aactaaaaga agaaattgag tctcgtgggt taacactaaa agtaattgaa
     4981 agcgtcaatg tgcatgatgc cattaaaatt ggattacctt cccgtgagca gtatatcgaa
     5041 aactataagc agacaatccg aaacttggca gaatatggaa tagaagtcat ctgctataat
     5101 tttatgcctg tatttgactg ggtgaaatct gaccttgact accgcttaga agacggctca
     5161 tccacgttag cttttatatc tgatgatatt ccagatgatc caagagaaat cgtcgcgcgc
     5221 atcgaacatt ccagcaatga atttggattg ccgggatggg aacctgagcg cttagctcct
     5281 atcaaatcct tgcttgaagc ctatgcatca gtagacgaag aaaaacttcg agaaaatttt
     5341 gcctattttc tccaatcaat catcccaaca tgtgaagagg taggggtcaa aatggctgta
     5401 catccagatg atccgccata ctcgattttt ggattagctc gaatcgtaaa gaatcgagaa
     5461 gatttagatt ggttgtgtcg catagtagac agcccttata acggaatcac tctttgtaca
     5521 gggagtattg cagaagatcc agaaaatgat gtatatggga ttttagcaga attttgccaa
     5581 agagatcgaa tcccatttgc acatgttcga aatattaaat ttttagctaa tcgagatttc
     5641 tatgaggcac cacataaatc cgaatacggt tcattagaca tgtaccgcat actaaaaaca
     5701 atgtatgaca acggatttaa cggatacatc cgtcctgatc atggaagaat gatctggaat
     5761 gagacaggaa gaccaggata cggcttatat gatcgagcat taggtgctgt ttatctaaat
     5821 ggattatggg aagcaatcga aaaaggaaac aaaagtaaag ataattagtg aaaaaaactg
     5881 taattttatt atgtaaataa gaaggatgtt caaattcttg atacataaat ccggaggttg
     5941 ttaataagca ttcggattta tgtatcttat ttggtctagg aagtaacttg attttctttt
     6001 ttcgattgat ccatgagtgc ttcttctttt aattcaaaga tatattctga gatcctcata
     6061 atcacttttt tccagtcttc tggtgtgatg attcctttta aaatatagat ttttttgata
     6121 tgagaaggca attgtttcaa agggcagtcg ctgataacga tgttgcagtt ctccataaaa
     6181 tcattttgta ttttgatcga ttcatttgaa aaaatttgtt ctatcttaac gatcagatct
     6241 ttactgatat aaaaatcacg cgtatagttc acaccaatca caattcgcgg ctgttttttg
     6301 tagcgatcat acaaatgtac taggtcttca accatccatt gcacattttc cttttctaaa
     6361 ataactggta acaagtgttg attttgttct ctgaaatcca tataaaattg ttcaatggcg
     6421 gtttggactt ctttcaatac gctatgttct tgataaagca tgaaagtagg aaggggcgta
     6481 tttggttgat tgaagttaag aaatccatta taaagtttat ttcgtgcgaa tgccaatgag
     6541 taaagaatct tttctttttc aggtataaac aaatcaaata cagtacaaag ggactccacc
     6601 atttctcttg aataaagaac atgaggaacg tttgcttttt ttaaaagttt gtattgctcc
     6661 aagcttgttt cttccgattc gattttcgtg atagaaagtc gagctaaaat attcactaag
     6721 atgcgttgtt cgcgacaaat tggcagatta ggtcgcaaaa tatctacaga gagatcaata
     6781 aaaaagtcaa tatattcata ctctgtgaat tctttttgta cgtctttcac agaagttgca
     6841 ggaaaattct ctttacatat ttttaataga aaatataatt tat
//
LOCUS       DAYYCY010000132         6644 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000132 DAYYCY010000000
VERSION     DAYYCY010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.132"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..859)
                     /locus_tag="ACHAZB_002186"
                     /pseudo
     CDS             complement(1..859)
                     /locus_tag="ACHAZB_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa10 family transposase"
     gene            1102..1557
                     /gene="tnpA"
                     /locus_tag="ACHAZB_002187"
     CDS             1102..1557
                     /gene="tnpA"
                     /locus_tag="ACHAZB_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFM8614822.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1822..1920
                     /locus_tag="ACHAZB_002188"
                     /pseudo
     CDS             1822..1920
                     /locus_tag="ACHAZB_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558064.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2143..2373)
                     /locus_tag="ACHAZB_002189"
     CDS             complement(2143..2373)
                     /locus_tag="ACHAZB_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HFM8614823.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(2603..3421)
                     /locus_tag="ACHAZB_002190"
     CDS             complement(2603..3421)
                     /locus_tag="ACHAZB_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM8614824.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            3582..4271
                     /locus_tag="ACHAZB_002191"
     CDS             3582..4271
                     /locus_tag="ACHAZB_002191"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFM8614825.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4285..5787
                     /locus_tag="ACHAZB_002192"
     CDS             4285..5787
                     /locus_tag="ACHAZB_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8614826.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            5800..6276
                     /locus_tag="ACHAZB_002193"
     CDS             5800..6276
                     /locus_tag="ACHAZB_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFM8614827.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(6373..6483)
                     /locus_tag="ACHAZB_002194"
                     /pseudo
     CDS             complement(6373..6483)
                     /locus_tag="ACHAZB_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2172 a         1032 c         1204 g         2236 t
ORIGIN      
        1 acttgtcctc gataagaatt gtagcgccgt gatttacgtg tgtatgtcgt tacttttatt
       61 cctagcttct tgatcaggcg ctgaactttc tttttattga tagagaaacc gcggtttctt
      121 agttcattgg tcatccgtaa acagccataa tccttgtgct tctcccgaac tttaagcatt
      181 tccgcttcaa tctcttgatc tggattttta cggtccaatc gtttttgcca gtacataaag
      241 attgccttag ggaactttaa agtttctaga agctcaacta atttaaacgg tcctcggagg
      301 cttgcgatga ttcgtgcgat tgcttcattc gtcgttgttg ggcttcctgt tttcgcagct
      361 tcctcaattc ttttaaaaag gcattttcaa tttgaaggga acggactcgt ttctccaact
      421 cctcgatgcg ttcacgttct ttagacgttg ctttagttgt agattttttc ttatttttgg
      481 gttcattttt cttagacaag ataggtggac atcctttcgt ttttgagagt ccatcgatcc
      541 caccctcacg gaattgacgc atccaactag cgatcaaatt agggttgttc atgttaagtg
      601 tgttggcaac ttcacgatag gacagctcac ttgttagata taactctatc gcatccagct
      661 taaattgaac agaatatttt ttcttttgtc gctttcttac tagaccctct tcaccaaatt
      721 cttgataggc attgatccag cgcttcactt gtgatggagc cttaatggaa tatttctttg
      781 ccagataatc acttccgcct ttaccatcca agtaatcatg aacgatcttt aatttaagtt
      841 caaaagtata cttagccata aaaaataccg acctccaaaa gttagatttt ttggtctaac
      901 ttttgggggt cggtacaaaa cttaaaagtt ttagaccctt ttattttctt aatgacttca
      961 gtcagttttg tgcgtaaagc gtgcgaaaca agaaaaccgc gcgtttctac gcgtggaaga
     1021 agtatcttta gcgataaaaa ctcctttcga tatactagtg agtggctacc aaccgctcat
     1081 cagatcgaaa ggagtttttt catgtcaaat gacgataaaa gtttagcata tactcgttgg
     1141 aattgtaagt atcatattat ttttacacca aaatatcaac gaaaagtaat ctatgggcaa
     1201 ttacgaaaag atcttggaaa aatacttagg aagttgtgcg aaatgaaaga tgtggaaatc
     1261 atagaagcac atgcaatgct agatcatatt cacatgcttg ttcgagtacc accaaaaata
     1321 tgtatatcca gttttatggg gtatctgaaa ggtcgtagtg cggtactaat ccacgagcag
     1381 cacgcaaatc tcaaatataa atacggaaac aaatcctttt ggtcgaagag atactatgtg
     1441 agtacagtcg gattgaatca aaagacaaat gaaaaataca tccgtgaaca agaagctgat
     1501 gatcgcattc gatatattat aagtaaaaga gagtacgtag aaccatttaa aaaataaaga
     1561 gaagtaagtg agtgaagatg agcagttggc ggtcttttaa aaatcccctt ttaaggggca
     1621 gcgagtgtcg accccttcta gggggattaa aaatacaccc gtcctcacgg gtgtatgggt
     1681 tactgataga cttgatttta actggtgtca gaaacttatg tccagaaagg gtgatgaaat
     1741 tttggcaatt tacggctatt caagaattag cacaagcaac caagattaca aaacacaaat
     1801 tcaaacatta gaaaatgctg gcgcagaaat aatcttccta gaaaatcact ggattatcga
     1861 acacctttgg aagtattttt gagttacgta agtattgatg atctgtctaa cttaatttga
     1921 caattaagag tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat
     1981 gaactaataa ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc
     2041 ataaactttt aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca
     2101 taacattgaa gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt
     2161 cttataaaca agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt
     2221 tatagaaaac attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc
     2281 agtaggttcc tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt
     2341 atttaagaga ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta
     2401 cacgtttttc tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc
     2461 aagaaccgta ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa
     2521 taagctatca agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta
     2581 ggtttagcta agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt
     2641 tttctcttaa ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat
     2701 cacaaatata aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa
     2761 tggctgtttg taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta
     2821 tagcaataat ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg
     2881 ttttcccaga taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt
     2941 gggcttgaaa atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat
     3001 tttctaaatc aagactagta tttccactag atcccactcc aaatatatat atgttttttg
     3061 attgagtgat cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg
     3121 aatagagagc ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat
     3181 gttctttttt ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag
     3241 aaaaaccaat tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct
     3301 taatatcact catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga
     3361 cttttttttc agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca
     3421 tacaaactcc tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact
     3481 cctaaatact ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag
     3541 ttgttatcca aaaataataa aaaattggag tgtttaggat aatggataaa aagaggtttt
     3601 tagatcttat ttcaaaaggt ttgattgttt cctgtcaagc attacctggt gaaccatttt
     3661 acaaagagga agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag
     3721 ttggtatacg tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac
     3781 caatgattgg aattattaaa agagattatc caccagaatc tccattcatt actgccacta
     3841 tgagagaagt agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc
     3901 ttagaaatcg acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat
     3961 atcctcatca gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata
     4021 aagcaggaat agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac
     4081 aagatgggcc agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg
     4141 ctgagggaag gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag
     4201 gtattgttgt gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag
     4261 cattaagtta gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc
     4321 atgttaccaa ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta
     4381 acaaatgaga gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt
     4441 ttaagtatta tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca
     4501 attggtattg ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga
     4561 gtagcttatc tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa
     4621 gatgcaacac tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt
     4681 ttacacaatc gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca
     4741 agatttattc cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta
     4801 atttggccac caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt
     4861 agtttaggaa cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac
     4921 cacaccattt accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga
     4981 tcaacaattg ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact
     5041 ggtctgttta cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttgga
     5101 ttaccagcag catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat
     5161 ggtgggcttt attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt
     5221 gaatatatgt ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga
     5281 ttatcttttt attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt
     5341 ttgatcgact ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa
     5401 ttaattcctt ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc
     5461 aaaaaattta aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa
     5521 gtaataaaca aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt
     5581 ggaaaagaaa atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg
     5641 aatggggaga ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa
     5701 gtgaagggtg gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc
     5761 aatcaaatat taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag
     5821 aaaaaacaag aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta
     5881 aatgatcctg tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt
     5941 tcaaaaattt atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct
     6001 attaatataa aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt
     6061 gaattacaag gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa
     6121 acctcgttgg ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt
     6181 atagttgttt ttccggagaa aaatcagttc aatgatttaa taatagataa tggtcgaatt
     6241 gctcatggga attcatgtgg aaaaatcact ttttaaaaaa gtgtatataa aaagctataa
     6301 aataagctaa ataggatatt tgtcaaatga agttggtgaa cagaaagaac tgttttcttt
     6361 ctgttcattt ttttatttta ttagtaatga ttccccatgc caaatcatga tccgtaattt
     6421 aaagttataa aagcttcgaa atccattggc atttcgttgg aataccttga tatgattatt
     6481 caaatataaa atatagagag aagagagaga gggaagatat ttataaaggg caaagttatg
     6541 tttactcaac ttaggaaata gaaatagatc aatttatatt ctcttgtcaa tcgaatatga
     6601 accttagcac tatataccaa atatagtaaa atgctgaaag atgt
//
LOCUS       DAYYCY010000133         6570 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000133 DAYYCY010000000
VERSION     DAYYCY010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6570
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.133"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            127..579
                     /locus_tag="ACHAZB_002195"
     CDS             127..579
                     /locus_tag="ACHAZB_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8614828.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            593..1282
                     /locus_tag="ACHAZB_002196"
     CDS             593..1282
                     /locus_tag="ACHAZB_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8614829.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1407..3758
                     /locus_tag="ACHAZB_002197"
     CDS             1407..3758
                     /locus_tag="ACHAZB_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HFM8614830.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3838..4233
                     /locus_tag="ACHAZB_002198"
     CDS             3838..4233
                     /locus_tag="ACHAZB_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8614831.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            4558..5175
                     /locus_tag="ACHAZB_002199"
     CDS             4558..5175
                     /locus_tag="ACHAZB_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8614832.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            5216..5515
                     /locus_tag="ACHAZB_002200"
     CDS             5216..5515
                     /locus_tag="ACHAZB_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HFM8614833.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            5549..5716
                     /locus_tag="ACHAZB_002201"
     CDS             5549..5716
                     /locus_tag="ACHAZB_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFM8614834.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(5761..6366)
                     /locus_tag="ACHAZB_002202"
     CDS             complement(5761..6366)
                     /locus_tag="ACHAZB_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8614835.1"
                     /translation="MGKIGYIRVSSQDQNLDRQLAMMEGLNIDKLFQEKASGKDTERP
                     EFQKLLNYIREGDCVIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQ
                     KRLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT         2265 a          910 c         1285 g         2110 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc aattatctta tcgtagatat atattataaa tttaataaga aagcgaggtg
      121 agaatcgtga aaaaaacgat actaagtatt ttttcagtat ttacaatatt aattgggggc
      181 tattttcttt tgaatttaga ttctgttaat tcaaacgctc aaataaaaga tcaaagagta
      241 gctgaacata ataataaaaa aatgagtttg accaaaaata tctattctat gtcagatgaa
      301 gaattgtata aagaatttgg tgatttatcc ggggaacagt tagaagaaag tattccagga
      361 attagtgact cagataaaat tgttgtcttg tctgctggtg ggcaattaat gggagatggc
      421 aaatccgaat tagttagtta taaacaaaat cacagacagt ttattgatgt taatactgat
      481 tccaacgcta tttcgattaa ggatctaaaa gagcttttaa atagtaaaag aaatgaatta
      541 aagcagataa aagaaactaa ttcagtggaa ttagagtaaa ggaagattca ttatgaaaaa
      601 gatttttggt gtttctgttg ggctacttat gatgttaaca tttggaaatg cagtatatgc
      661 tgctgagaaa gaagttattt cagatccaga gcagtacgcc tcacgatact ttgctgattt
      721 tgaagaagaa gataaagtac ttgcattgcc agaaggtggt tttttaaaag gggaagctaa
      781 aattgtatct atggataatc cagatataat tcaaggttca tataattcag aaactgatcc
      841 tgcttcagtt tcagtgacag aagcaaaaaa aattgttgct gaaaaattca aaaatgatat
      901 tgaaactact ccaattatgc ctcgaggagc atttccaccc gatgttggta ctagcggaca
      961 ccttcttgta ctagcaggag gaggaagcta taaatcacag ccatttagtg gtgcgggatg
     1021 gcgttttgga ggtatgggtg taaaaccagc tgatggtaca ggcaactttt tacgttggaa
     1081 atcctacatt gacgatggaa gagtaggaaa tgtaaatcaa gccaacgcaa ctaaatctgg
     1141 ttctattcaa ggaatggcat tgtatgctcg tgaaggattc aaatactttg caccaaacgg
     1201 gcctggttgg acaatgaata taggtattta ttacacttat aatcctgttg caggtacgta
     1261 ttatgttgtt caaaatattt aataaaacag cacaaagaga tttctttgtg ctgttttatt
     1321 atttttagtt gttgacaaac actaaaatat aaaatataat caacacataa acgaacgtat
     1381 gttcggcaag aaagcagggg atttttatgg atgagatggt tttaagtact cagaaatggc
     1441 taaacaagaa atacagtaat gttacagggt ttgataaggt tccagaaaat gggcgaacag
     1501 ggtggcctac aatctatgga cttattgaag gactacaagt agaacttggt attactaatc
     1561 tagtagctaa ttttggacct accactgaaa aaatgtacga taatcaggtt actcctaaat
     1621 ggggcaaaaa tctacctaaa aatattgtat ttcttattca aggtgccttt tggtgtaagg
     1681 ggattaatcc aggtggcttc gatggggtat atactccata tttagatact gctgtaaaag
     1741 agttgcagac tgatgcaggg tttaatggaa gcgctgtaac tggagttttg gatgcgaaat
     1801 gggccaaagc gttatttgat atgagtgctt ttgttcttgt tcccggcgga gatagtaaaa
     1861 ttagaaaaat gcagcaatgg ttaaatgtaa attatttaga atacacggga ataatgcctt
     1921 gtgatggtat ttatcaaaga aattcaaatc aagcattaat ttttgcatta caagctgaac
     1981 taggatattc tcccagtgaa gcaacagggt cttatggtaa tgggacaact agtaaaactt
     2041 atcctgtgtc tgaaggtaac tcgggaaact atgtaagaat aattcaatat ggattatatg
     2101 ttaatggtta ttttgaagac ggtacattcg atggtatttt cactaaatat atgggtttag
     2161 aagtactggc attccgaaaa tttatggtat tgcctgaata tactgaaata gcagatgttg
     2221 ttgtaataaa aggactattg tctagcgctg gagatattag aagatctgca gatggcgcag
     2281 atacttctac gcaattaact agatctcaaa tccaaacatt ggttgataat gatataaaaa
     2341 tagttggtag atatcttact ggtactgttg gaattggaaa agatgaacga aataagtatc
     2401 taactacaga ggaattgaat aatatttttt ctaaaaattt atctgtgttt cctatctatc
     2461 aagatggagg tgcagcatta gcttatttta cttatgatcg tggcttatca gatggtaaga
     2521 aggctataga tgcagcaaaa aatctaaata taccattaac aacagttatt tattttgctg
     2581 ttgatctaga catgttaggg gaagaaattc ttgcatatgc tggagaatat tttaaggggg
     2641 tatctgctgt aatgtcatat agcgggtatc aaacgggtgt ttacggaaca agaaatgttt
     2701 gttctcaaat aatcaataac gggtatgctt cgtttgcatt tgtttcagat atgtcgactg
     2761 gttatagtgg gaatttaggt tttcctatgc ctagacaatg gtcttttgat caatttgttg
     2821 aatttaccat aggaagtggc aacggagctg ttggtatcga tttaatcgct acatcgggta
     2881 gagatagcgg atttaatgag ttatcaaatg atactaataa tgataattac attgccaaat
     2941 acaatcagaa ggttataaat cagatggtac gtgcgtatca atatctatca caagctggtc
     3001 ttgataatcc ttggaaccct catttgacat tttatagata tgtaaattat tctggtttgt
     3061 catgggacat catttcttct cctgtaacag agcatgatag aaaaatttat gatgaatata
     3121 ggaataaatt aactaatagc gagggcctgt ataattattt tatagatcct aatactggtt
     3181 caataatagg tttgcctcac ttaattgtga cgttacaatc acagatgttt ctagccgaca
     3241 caatcaatga tagtgtttct gactttgcag gttggttagg agatttaatg acttgctggg
     3301 gtgaagttaa gaaattagga atacaaatgg agcaaggtgt atttgcatta gtaggtacgg
     3361 ctgcaggtgg aacatttagt ctagaagatt tataccaaga tgttgacgcg gtaaatgttt
     3421 ataccttagc aaaaagaaat tctgattcaa cagctttatc ggctttaaaa acgtactatt
     3481 gtgataaggg tgctattgat agatttaatc aatttgttaa gaacagattt ggttcttcag
     3541 ataaaatcgc ttcagtaaca aatagtttat tagaaaatga cgaaacagtt gatatcttag
     3601 gtgtagggat aatttcattt ttcattgata aaatagttaa taattcactt gggacagatt
     3661 ttacttatct tgattttatt ggagatgagg ggaaagttgc aaatggattt tctgaaaaaa
     3721 ttattcaatt tcaagaaaat agaagtaaac ttaattaaga atcgtttact atttaaacag
     3781 tgtccttttc ctttttaaat atatacgata aaataagtat atgaattgta aaaggacatg
     3841 atgattatgg aagaaataga atggaataat ctgactcaaa ataataaaaa taattctgga
     3901 tgtggaatta tctttatgtt tgttatagtt ttgggaatag tagtactttt aataactgtc
     3961 aagatagtag attactttgc gaatactcct gaagaacaaa tacagagaaa acttcctgag
     4021 tttgaaagag taccacctta tgatccaaga gataattcat ttcaagtaaa aaaattaaaa
     4081 gagtctccaa atgaaatgtg gtttgaagtt acagttgaat caagcgactt caaagaaaaa
     4141 aagaaagaat acaattatat tgtaaaggca aaagtatcaa aaagatttat ttttaaccct
     4201 aaattagaaa tatatagtgt tgtaatagaa taatagtgtt aaaaaaattg taaaagttaa
     4261 agaagtcaaa ttctgatgtg aaaaataaaa tataccatgg aagacataaa agaaaaactg
     4321 gataactagt ttatttaatg gcagaccgtt aaggcctgcc atttttatgt attttagcgg
     4381 aataaatata atcatgcggg agtgtaaact tactgtttca aaaagtatac tttttgacat
     4441 gccttgattt tctttgttta tgtatggcat aataaccaca taatacaatt tgaaacgttt
     4501 ctgatttaat ctgattgttt tgaaacaatt ttagtgaaaa aagggtagat aaaaacgatg
     4561 agaaaatttt tttatatccg ggtcagtagt tatgatcaat cgatcgaacg acaactgatt
     4621 tccgcgaagg aattaggaat ccctgaagaa tatatatttg tagaaaaagc cagcggcaaa
     4681 gattttaaac gccctgaata tcaattaatg aaacggatgt ttcgaaaagg agatttactc
     4741 tatctacaat cattagatag actaggcaga aataaacaaa tgattttaga agaatggcga
     4801 gaattaacta aggaaaagca gattgatatt tctgtgttag acatgcctct gttagatact
     4861 gctagttaca agcaactaga tgggttagag acgcttatct ctgacttaat tctgcaatta
     4921 ctcagctata tggccgaaga cgagcggaaa cgaattaata gccggcaaag agaaggcgta
     4981 gaagctgcga aacaaaaggg tgtaaaattc ggcagaaaga aaattgcttt acctcctgaa
     5041 tttcctgcga tctattctga ctggaaagca ggaaagataa ctgcagtcta tgctatggat
     5101 caattgggca tgaaacgaaa taccttttat cgacgggtga aagaatatga acataccttg
     5161 caccactttt cttgatagat ataacagcta ttagacggga agggagagtt tgaaaatgga
     5221 cttaacgaat caaagggtcg tttttactgg aacgctgcag caaatgacac gaacccaagc
     5281 catcggtttg gttcactctt taaacggcca ttgtcaatca agtgttactt caaaaacaaa
     5341 tttcctggtt tgtggtcagt attctaagtc gttgtttgat gattctctct atacaaaaaa
     5401 ggaacaaacc gcgcgggact tgattgcgtt aggtcatgag attaccattt tatcagaagt
     5461 cgaattttat gcgttaatta gtcaacagtt gaaagaatgg caacggtatt tataatcgtt
     5521 agtaaaattt acaaggagga aaatttttat gaagcgaaaa tcttatgatc gttggggcat
     5581 tgaaataact agttttaatc gaaatattgc ttttcacgtg gagtgtgact gtggggaatt
     5641 agcaaaaagt gtttatcaaa aaaattactt ttactgtcct aaatgccatc gccgatacgg
     5701 ggtacatgga acatagaaac gaaatagtgc ggtagaaaag cgaatgaatg tagtatattt
     5761 ctacaaatca ttcgctttct ttattttata aatagttggt cgagagatcc cgttttcttt
     5821 tgcaatctct gcaactggaa tgccttgctt taacatgtcc accacggttt taaaaactaa
     5881 gcgtttttgc ggatctgttg agttaataga atactcgatt ggtcgtccct tatatcggcc
     5941 ggctttttga gccaataata ctccttgccg ctgacgttca cgaattttct cacgctcgtt
     6001 ttgtgcaata taacttagca gtgataaaaa catatcgaac atagcggtat ccaggagttc
     6061 atttccagta ttaaaatcta aaaactgtgc atctaatatt tttaacgcta cttttttctg
     6121 tttcataaaa gcaactgtat tttttatatc ttcataatct cttcctaaac gatctagtga
     6181 tgtaacgatg acgcaatcac cttcacggat ataatttagt aatttttgaa actcaggacg
     6241 ctcagtatct ttaccactgg ctttttcttg gaataattta tcaatgttaa gtccttccat
     6301 cattgccaat tgtcgatcca aattttgatc ttgtgaagat actcgaatat agccaatttt
     6361 tcccataaaa aactcctttt taaaagtgat ataatttaca tgtaaaattt acttgtagtt
     6421 ttactgctat catagcattt ttaaaaataa attttatgta gacttaaaat ttacttcatg
     6481 gagggtttaa atggatgaaa tcaaaagtaa caaaaaagca tttggcattt atttaggaga
     6541 acctagtaca gaacaattaa accaattttt
//
LOCUS       DAYYCY010000134         6521 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000134 DAYYCY010000000
VERSION     DAYYCY010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6521
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.134"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            41..778
                     /locus_tag="ACHAZB_002203"
     CDS             41..778
                     /locus_tag="ACHAZB_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM8614836.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            792..1607
                     /locus_tag="ACHAZB_002204"
     CDS             792..1607
                     /locus_tag="ACHAZB_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFM8614837.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            1620..2267
                     /locus_tag="ACHAZB_002205"
     CDS             1620..2267
                     /locus_tag="ACHAZB_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFM8614838.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            2283..2939
                     /locus_tag="ACHAZB_002206"
     CDS             2283..2939
                     /locus_tag="ACHAZB_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFM8614839.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            3101..3922
                     /gene="racE"
                     /locus_tag="ACHAZB_002207"
     CDS             3101..3922
                     /gene="racE"
                     /locus_tag="ACHAZB_002207"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFM8614840.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            3925..5280
                     /gene="rph"
                     /locus_tag="ACHAZB_002208"
     CDS             3925..5280
                     /gene="rph"
                     /locus_tag="ACHAZB_002208"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFM8614841.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            5281..5799
                     /locus_tag="ACHAZB_002209"
     CDS             5281..5799
                     /locus_tag="ACHAZB_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM8614842.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            5906..6406
                     /gene="cbpB"
                     /locus_tag="ACHAZB_002210"
     CDS             5906..6406
                     /gene="cbpB"
                     /locus_tag="ACHAZB_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0180001 - cyclic-di-AMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP-binding protein CbpB"
                     /protein_id="HFM8614843.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         2148 a         1095 c         1441 g         1837 t
ORIGIN      
        1 taaaagaaat cttactaaat aataatggga gtgtaattct atggcaatga ttaaatttga
       61 tcacgtggat aaatattatg gcaagttcca cgcattaaaa aatatcaatc tcgaattcga
      121 gaaaggagaa gttgtcgttg tcattggtcc ttctggttca ggaaagagca cgatgcttcg
      181 ctgtatcaat ggattagaga cgatctcttc aggtaagctg ctgattaatg atactgactt
      241 gcatgataaa aagacaaaac tgacagaagt ccgaaaaaac atcggcatgg ttttccaaca
      301 tttcaatttg tacccaaata aaactgttct ggaaaacatc aaattagcac caatcaaagt
      361 gttgaagcaa gaccaggcaa ctgcagtcaa aaacgcggag aaatttttga aaactgtcaa
      421 tatgttagat aagaaagatt cttatccttc catgttatcc gggggacaac agcaacgggt
      481 ggcaatcgcc agaggtttag cgatgaatcc tgagatgctg ctgtttgatg aacctacttc
      541 tgctttggat ccggaaatga ttggtgatgt attagacgtt atgaaaaaat tggctcatga
      601 cggtatgtca atgattgtcg tgacacatga aatgggcttt gccaaagaag tggcggatcg
      661 tgttatcttc atggctgatg gagaagtatt ggaagacagc agaaatgtga gagacttttt
      721 cgaaaatcca aatgaagtcc gagcacagca atttatcagt aaagttatca atcattgata
      781 ggaggaaaaa gatgcgcagg ttaaaaaaaa tacgcctgat tttcctttta ggcattgctt
      841 ttcttgcact agccggatgt aaaggaaatg ggttggcaaa tgaagatatt ttagagcgaa
      901 gcaaatctac caatgaaatc atctggggtg tcaagtatga tactcgatta tttggaatga
      961 tggatattga gagcagaact gtacaaggat ttgatgtaga tattgcaaaa gcaatcacta
     1021 aaaagatcct tggtgacaat ggaaaaacag aatttgttga agtcacttct aaaactcgga
     1081 ttccattatt gaaaaacgga aatatcgatg caattattgc aacgatgacg atcacagatg
     1141 aacgaaaaaa acaggttgac ttttcagatg tctatttcga tgctggccaa gctttgttgg
     1201 taaagaaagg aagccaaatc aaaagtgtag atgatttgaa tgcttctaca acggttctag
     1261 cagtcaaagg ttctacatcg gctgcgaata tccgacagca tgcaccagat gcaaaaattt
     1321 tagaattaga aaactatgca gaagcattta ctgctttaca atctggtcag ggagacgcga
     1381 tgactacgga taatgcgatt ttgttaggga ttgcagatga aaatccagag tatgagttag
     1441 ttggtggaac gtttactaat gagccttacg ggattgcgat caacaaaggg caagaaaact
     1501 tcttgaaagc tgttaaccaa gcgctagagg aaatgcatgc agatggcaca tacgataaaa
     1561 tctatcaaaa atggtttcca aatgaaactg aaggaaaagt agagtaaggg ggagaggcaa
     1621 tgagcgaatt gatacaaact tatggaccaa tctttgtcga cgggttcaag atcacattgt
     1681 tttcgagtgt cttagcactt gtgtttagtt tgattatcgg aacgttaatg gcaatcttcc
     1741 aattatcaaa gaataaagta gtgagcgcct tagcaaaggc atatgtagaa ttttttagaa
     1801 acattccatt attaatcatc gtgatgtttt tttatgttgt cgtaccgatt tattggttta
     1861 atctagatgg attccaagca ggtacgattg gattaacgat ctatacatct gcttttattg
     1921 ctgaaacagt acgttcaggg attcaaaccg taccaaaagg tcaaacagaa gcaggactat
     1981 cttctggttt tacctattca gaaatcatgc gttatattgt tttaccgcaa gcattcaaaa
     2041 tcgttatccc gccattaggc aaccaattta tcaacctcgt aaaaaactca tctattttag
     2101 caatcgtagc tggtttagat ttgatgtatc aaggagactt gatcgctagt gaaacattca
     2161 ataccttcga tacgtatatc atcgttgggt tattttactt agttatcacg ctaccgttat
     2221 cttacttaat ggtttatctt gagaaaaaat gggcggttcg ttcatagaag gaggggaaat
     2281 acatggattt tagcggcgct ttttcatgga tgaatatccg ttttttattg gaaggattaa
     2341 aggttactgt tgaagtttca ttgttttcga tcatttttag tttcttgatc ggtggtctga
     2401 caggtgtgat tcgttttgcg aatatccctt tcttttccaa aatccttggt gtagtgattg
     2461 atattatacg gaatcttcca ttattattga tcattttctt cacttacttt gccttacctc
     2521 aaatcggcat tcagatgaac atcttctggt ccgctgtcgc agcattaacg atttttgagt
     2581 cagctatgtt gtcggaaatt ttccgagctg gactcaatgc tgttccaaaa ggacagatgg
     2641 aagcaggatt atcaacggga ctgacttatg tagaatcgat gcgaacgatc gtgttaccgc
     2701 aagcatttaa atcaatgatc ccggcaattg tgagccaatt gatttcactg atcaaagata
     2761 cttcattagc agtaatcatt tctttacctg aactttctca tcaggcacgt attatttatg
     2821 gacagaacac caattatgtg cttccgatgt tcatcatcat gacacttatg tacttcgtgg
     2881 tatgttacgc gctttctctg ctttccggtt acttggaaaa gagacggtac agttattaat
     2941 agatgattct tttagcggtt gtggcaaact aaagtcacaa ccgcttttcc tctttccatt
     3001 cgaaaaaaag agatttcgat tgttttaagc aagtaagagc acttgaaaat tcacgctaaa
     3061 aagtacacaa tagggaggat agaatttatt gaaaaaaagg atgatacgat tgacagataa
     3121 tcgccctatc ggatttattg attcaggtgt cggcggcttg actgtagtaa aagaagccct
     3181 gaaacaatta ccgaatgaaa atattttatt tgtaggagac acagcacgct gcccatatgg
     3241 ccctagaccc gcggaacagg taatccagta tacttgggaa atgacggatt atctggtgga
     3301 gcaaggaatc aagatgctgg tgatcgcctg caataccgca actgcggtgg ctttagaaga
     3361 aatcaaagct gctctttcta ttccagtcat cggtgtgatc cttcccggta ctagagcggc
     3421 agtaaaaaaa acacaaaata aacaagttgg cattatcggt acgattggta cggtaaaaag
     3481 tcaagcttat gaaaaagcac tgaaagagaa agtaccagaa ttgactgtga caagtcttgc
     3541 ttgtcccaaa tttgtttcag ttgtcgaaag taatgaatac cattcatcgg tggcgaaaaa
     3601 aattgtggca gaaacattag ctcctttaac cactaaaaaa atcgatacat tgattttggg
     3661 atgcacccat tatccattat tacgccccat cattcaaaat gtaatgggag aaaatgttca
     3721 actgatcgat tctggagcag aaacagtagg tgaagtatct atgctgttag attatttcaa
     3781 tttgagcaat tcaccgcaaa atggtcggac attatgccag ttttatacaa ctggctctgc
     3841 caaacttttc gaggaaatag ctgaagactg gcttggaatc ggacacttaa atgtagaaca
     3901 tatcgaattg ggaggaaaat aaggatgcgt catgacggga gaaaagccca agagcttcgc
     3961 aaactggagc ttcaaacaaa tgttttcaaa tatccagaag gatcggtagt gatccgtttt
     4021 ggtgatacaa cagttatttg ttctgcaaca atagaaaatt ctgttccccc ttttttgaga
     4081 gaaacaggaa caggatgggt gacagcagaa tatagtatgc tgcctcgagc aacgagtacg
     4141 agaaatcgta gagaaagcag caaagggaaa ctttctggta gaacaatgga aatccagcgt
     4201 cttattggtc gttccttgcg tgcagtagta gacttggaaa aattaggaga acgtagtatc
     4261 atcgtagact gcgatgtgat ccaagcggat ggcggtacta gaacagcaag tatcactggg
     4321 gcatttgtgg cattgaaatt agccattgaa aaacttttaa gagaaaaaga attgtcagaa
     4381 gatccaataa aagaacatct tgctgcagtt agcgtaggaa tcttaccaga tgggacttgc
     4441 gtcacagatt tggattacca agaagattcc gcagcacttg tagacatgaa tcttgtaatg
     4501 acagaatctg gaaaattcgt ggagattcaa ggaactggag aagaagcaac gtttgatggt
     4561 gaacagttga atgaaatgct cttttttgga aaaaacgcga ttgaagattt gatcaaagag
     4621 caaaaacatg ctttgctaac agagtttgct caaaatgatg agcggataga agaaacaaaa
     4681 acaatcatca ttgcgacaag aaatccagga aaagcagaag agtttcgaaa tatgttcaaa
     4741 gaagcgggat atcatgtaaa aacattactt gactatcctg aattgccaga tgtggaagaa
     4801 actggcagca catttgaaga aaatgcccga ttaaaagcag aaacgattgc acaattgctt
     4861 gatcagccag tcttagcaga tgattcaggt ttgaaagtgg atgctttagg cggtatgcca
     4921 ggaatctatt ctgcacgttt tgcaggggaa caaaaaagtg atgcggggaa taacgccaaa
     4981 ttattatatg aattgacaga tgtgccggat gaaaaaagaa cagcacaatt ccattgtacg
     5041 ttagtattcg cggcacccaa aaaagacagc cttgtagtag aagcggaatg gcccggtcga
     5101 gtagcccgta taccatctgg cgaaaatggc tttgggtatg atcctttatt tatcccagaa
     5161 ggtaagaaac aaacagcagc agaattatcg agcgaagaaa aaaacaagat cagtcatcgg
     5221 gctcaagcaa tgaaaaaatt gagtgccgag tggaaacaat ggctggaagg agagagatag
     5281 atgcggtatt tagtagtaag tgataatcat ggagacagag atatcctcgt tgacttagta
     5341 gatcgttatc gttctgaagt ggatatcttt ttccattgtg gtgattcgga attagaagct
     5401 tctgacccat tatgggatac attccaagtg gttcaaggta attgtgatta tggaccagga
     5461 ttcgaacaga aaaaagtgat ccaaactggt caggatacag tattcatgac tcacggtcat
     5521 ttatcgaatg tccgatttgg tttgacacag ttagcaatcg aagctcgagc tgcaactgcc
     5581 gatatggtgt tttttggtca tacccatcaa ataggctgtg agatggatca gaaaatcctg
     5641 tttttgaatc ctggtagcat ttctcagcct agaggcccga tccaaatccc atcatatgct
     5701 cttatcgaaa gtttagatga ccaaatttct gttcaatact ataaccgtgc ccatcaggca
     5761 gttgaagagt tggccttttc tttttcgaga gagcgctgaa taaaaaaact ctaattttta
     5821 ataaaaataa gctttatttt atgaattttg catccggttt cggtaaaata gtagtgttga
     5881 atgaaataga aatggaggcc atcagatgat tggacctata gtaagagaaa tgctgctgga
     5941 aaatcaggaa acgtttttga ttcctgcaga aaatgtggca aatgttatgt atcatcatcc
     6001 attgtcccat ggtttacttg tgttatcgaa ggtcggttat tccaaaattc ctgtattagg
     6061 aaaagatgat cactttgtag gactagtaag tttatcagat gtggtcaata aaatgattga
     6121 cttgcaaaca atcagcatgg acccgttaga aggactaacg gttgctgatg tgacagaaac
     6181 agatgttccg acaattgatg aaaattggga actagaagaa gtactccatt tgttagtcga
     6241 ttcgcctttt ttaccggttg tcacagaaga tcggattttc aaaggaatca ttacaagaaa
     6301 agagctgctt aaagccgtaa acttcatggt gcatgaatta gaacgtcgta atatattgtc
     6361 tccgaaaatc gaagaattaa aagaacaaat cgatatcgtg agctaaagaa cgagaaacta
     6421 aaagataatc aaaaaataag tagaaaaaac agcgtaccag ttttgacttt atcttgaaaa
     6481 agaggctggg acaaaagcgt ttaactccaa gaaataagaa g
//
LOCUS       DAYYCY010000135         6457 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000135 DAYYCY010000000
VERSION     DAYYCY010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6457
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.135"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            415..621
                     /locus_tag="ACHAZB_002211"
     CDS             415..621
                     /locus_tag="ACHAZB_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HFM8614844.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(823..1824)
                     /locus_tag="ACHAZB_002212"
     CDS             complement(823..1824)
                     /locus_tag="ACHAZB_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8614845.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(1829..3742)
                     /locus_tag="ACHAZB_002213"
     CDS             complement(1829..3742)
                     /locus_tag="ACHAZB_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM8614846.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            complement(3910..4416)
                     /locus_tag="ACHAZB_002214"
     CDS             complement(3910..4416)
                     /locus_tag="ACHAZB_002214"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFM8614847.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            4576..5016
                     /locus_tag="ACHAZB_002215"
     CDS             4576..5016
                     /locus_tag="ACHAZB_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFM8614848.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            5175..>6457
                     /locus_tag="ACHAZB_002216"
     CDS             5175..>6457
                     /locus_tag="ACHAZB_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFM8614849.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRS
                     KHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTK"
BASE COUNT         1833 a         1314 c         1047 g         2263 t
ORIGIN      
        1 attatttttg tttcttattt tctaaaaaat taaaaaaaat tttggtgaat taagtatttc
       61 cttgataaca ctggcttttg gcctcatttt aggttatcat ttgtaatttt atttcacagt
      121 tgatatgatt gttatgtaaa ataaaaaagt ttaggataca tcgaattttt ggggaaattc
      181 ttttgggggt atcctaaatc ttttctatta tattcccata ttttctatta ttggtctaga
      241 cgtatgatgg taaactttgt acctatctga ttgctgaatc attaattgct tatcattagc
      301 catctttgtt tcaagttcgc tagatgatca ttttattcac atcctaacaa acttgtcaca
      361 tttatagatt cttaatctat ttatcataca attataaaaa aggaggtgct gcttatgaaa
      421 gctatccata tttctttgct ttttttcgct agtgcattga ttttatttgc tattcatctt
      481 tgttcccagc cttatatcaa taacaacgga atattagtgg aaccagcatt cttttgctta
      541 ccactcggct ttttgtccct tcttgcctcc gcaggcttcg gatttgttgc tttttttaaa
      601 cgatcaaaac aacaaatctg acttataaaa tttacgtaaa agagatcgtg acaataatcg
      661 tgtgatattc ccaaatttcg aataaaaggt gccccttgcc atctctaact cctcagtcac
      721 aagttacaat attcataaac catgaaaagc ttgtttattg aaattgctcc ttgtgacttg
      781 gagctaagtg acagattaca acctcttttg catcataaga ctttactcct tgaaaaacat
      841 tactaacttt tgatatgttt cttgcgcttg tttattgctg aaatcaaact gatattcatg
      901 gttcaaaaca gcatttttct ccgcataatc tacttttgtg actttcaccc ccgctttctc
      961 aaatacagaa gttacttctt ccatttgttt cgtaaacgta tttgtatttc catccgttaa
     1021 gaatgtgttt tgcgtccaac gatccgcatg tctgaccaag gctaaacttt tgaccttctc
     1081 tgattgttca aagtccttat tatcaaagta agcccatcca catttttcat aaagaaagct
     1141 agagatttta tcacttactt gagtaagtgc agaaggatcc aacagaccag acaaagaaat
     1201 aaacttcgta atctgcttgt tctccaaagt cggatcaaga tgatcagctt ttttaatttc
     1261 ttcattttga agtgctaaca aatattctcc actaatttgt gcacctgccg aatttccacc
     1321 aatcgaaata ttcgaccaat ctgcttgatc ttttatctct tttttagctg cttgaatggc
     1381 ttcgttcaat tggataactg gtgtaggatg ttttgcttcc ggtgatcgtt cataatctat
     1441 atttacgatg atttgctgcg tgtctgaaca tagatattcc aaataatcac gacaatcatt
     1501 tttatcccct cctacatatg cgcctccatg catccagaaa agaatcggta attttttctt
     1561 ttccactttc ggtgtgtaga tatccatatt attatttttg tactttgaag gataggatat
     1621 ttcttttttt atatcgattt gttcgatttt ttgctggaaa tcatctggtc gggcatatac
     1681 ttgttctttt tcacttgtgt caaattgttt tctaacaagc aagctaatcg gctttggtgt
     1741 gatttgatta atcaaaaata cagtgactaa aataactacg atgataactg gtactgcaat
     1801 cactattcgc ttgaaccatt tcattcattt accctctctt tcttcgccaa atccatgtga
     1861 taatcgtgct aacagataaa aatccacctg aataaatgag aagcgaatgt ttcttttctc
     1921 cagttttcgg caacattctt ttttttgcat ctgtcttttt ttgtactttc actgtcatct
     1981 gaatcggttg cgtatcatca cttgattgat cctctcgagg ttcattcttt cccttattgt
     2041 ctgcattctc tgtcggatct tgcttttttg gtggagctac tgggattttt ttgtaataga
     2101 aaataatagt tggtatcgtc tcattaaaaa tcccttcagt cggtccttca acatttgcta
     2161 attcgtatcc ttcgaatgtc ttttgctctg tcatataatt ttctccataa gctccgtaaa
     2221 gctggatttc gtcctggtct ttgagtagac ttttattgtt ttcatccaca tactttacgt
     2281 ttatctgatc atggaacact actttcaaaa agtccgttac atgtttcgtg taggcagcat
     2341 ggtcaacttc ctgtgccttt ccatgccctg ctccttctac taataaaaga tctttatacc
     2401 ctcctgataa ctcatataat ttttgcgcgt tagacacagg tactacatca tctgctgttc
     2461 catggataat caatttaggc aatgaatctg acattccaat cgtaggtaga gcagcttttg
     2521 tatccaaatg catctcctga tcaaaatagt attgtttcaa aaggcccata aataaatctt
     2581 catgctcttg atccccaatg atgtctaact gattttttat ggcatccggt agtgcggggg
     2641 ctactgtctg gctgtacgta aaacgtagtt gttcatcaat tgatgcaaat ccgcaatttt
     2701 caataattcc tttgacattg acaggaatat ctttcgctaa tacagacatc atcgtagctg
     2761 ctcccatcga accaccataa agtaaaatct tttgagatgg ccgctctcga acttcttgat
     2821 ttatccagta aaggacgtca tctgactcat attgtccata ggtcacataa tcgcctccgc
     2881 tattaccagt cgctctggaa tcaacaaata ataaattata cccggcctta taaaaatcat
     2941 tgtattcttc tgtaacgatc ccgttattcc aattccctcg aaatccgcca tggatcatga
     3001 ctgttttatc tgaaccttga tcgacataat atgcatatac atccatcatt ttgccatctt
     3061 gtttgtttag tacagtttga tgaagttgtt ttgtatttgg atagaagcct gtgttttctg
     3121 taggcgttgt cccatcatct gtacctccaa gcatcgacat caacacactg ttttttgcaa
     3181 aagcgatatc gtacaataga taagtagcaa tcagatgttt gataatctct ttatctacac
     3241 cataatcatt aacagcaagc tcagtgacta ataactctat taattttgac ttgatgtctt
     3301 ctgttggtcc attaagtatt tctgtcatct gtttaccgaa gtaattcaaa tattctttac
     3361 cattgaaatt ttcaccttta ccgatatcat cattttctgt cccattctct ttgtcactta
     3421 ctttagcaga agaggattcg tcgtttgaca tttttgtagc tgttgcttct tctgtttgtt
     3481 ggttatctgt ggtagcggtc gtttcttgat ctgtgctagt aacagatgtc tcaaccgttg
     3541 tttcagtatt tgtttctgtt tgtgaagtac tcgatttcgt ttcatttcca gcatcagtag
     3601 tttctgtggt ttctacctcc cgagattccg attcggaaga aatgctctct ccatatactg
     3661 tccattgcgg gacgcttgta cttacagcaa ataagatgac caacgctccc tttaatactt
     3721 gttttgttct caagctattc actccttttt atgtttctag gagaattata tgtaaacgca
     3781 aacatttttt atacaaaaaa taaatggttt atcccataaa aatagaacag gattattttc
     3841 tcattttacg taataaagca gaatggataa accagtatcc attctgcttt ttttaaacga
     3901 agaaacaact tattgctcca aaaatgtcag aaatcgttta tcccaaattt tctcgtaaaa
     3961 agcgattgtt tcctttgctt tcataaaagg attatcataa gttgtcgttg ctcctctaca
     4021 catcataagc tggtatccta atccatgagc aaacttcaca gtcgtatcta tacaatactc
     4081 tgcttgtgcc ccacataatt cgatcgaatg acagtccaat tcagttaata aaacactcaa
     4141 attagtctta taaaatgaat tcgcatgggt ttttctcaca aaataactgt gctcaggaat
     4201 agataattcc gacaatagct cccatggttt tgtttgtggc actaattgag catcttcgtg
     4261 ttgaacgaaa ataactggtt tcccaatcgt ttcgtatgtt ttgattcttt tattcactaa
     4321 tgctgtcact gtttctaatt cgtgtatcgg cgaagaatca aaacataccc ctttttgtaa
     4381 atcgatcact actattgcgt cggctgtgtt tgacatactt gtgccttctt tcttttttct
     4441 tgatttaata gtatttctta tctagctagt tccattatac gtgacaacat tttcttattt
     4501 tacacctcta aatggcactt ttcgactatt tcttcatgtt attatttcat aaatacttag
     4561 ctattggagg aatgtatgat gattaaattg tatacaggtt ctggctgctc gtcttcaaga
     4621 cgcgcaaaac gttggcttat cgtccatgat ataccgttta tcgaacagaa cttaattcgt
     4681 caaccactaa ccaaaaatga atttatccac ttactaaaat tgacaaacaa cggcgtgacc
     4741 gaaatgctct ctactcattc tattagttat aaaaatcttg ttcacagaat agatgaactc
     4801 agtttgagtg aactatatat tgagattcgt acatcccctt ctttattgaa aaaaccgatt
     4861 atctacgacc aatctgcaaa acttttgact ggatttagcg aagataaaat caggatgttc
     4921 ctttctaaaa ctaaaagaaa gcaagagctc aacgaactct tgctttctgg tcatcttgtc
     4981 gattttaaag aaaataatct ttttgcaatg tcttaatttt ggcataaaca atctgctttt
     5041 ctggatctat aatatctagt gttggtgacg ccttctgttg ccaatacttt ttcttttttc
     5101 taataaacgc aaacagacac cttgaaatcc tttagaataa agtcgacgaa aaccattcaa
     5161 aggagtgttt caagatgtcc tattcagagt ttaccaaaga gttactagat attctagacc
     5221 taaatcttac ctttcatgaa gatgcctttc gcaaagagcg cattaatgac gaaacatgtt
     5281 ttgtttttga tggcacattg acctatcaac cagaggaatg cttccattgc cactatcaaa
     5341 ataagcaaac gatcattaaa tggggctgga aaaaagtatc catcttattg aatgatgtca
     5401 gtaactacaa gaccattgtg cggattaaca aacagcgctt caaatgtaaa cactgtggaa
     5461 agactttttt agcagaagat tcagttagcg atcgtcgctg ctccattgcc cgcagagtca
     5521 aacaagccat tcttgagcta ttaagtgagc ctatttctat gtctttgatt gcacgtatga
     5581 agcacatttc tccgaccact gtcattcgaa tacttcgcag ccttcggcct aaaaccgttt
     5641 ctttgaacca gccgttacca gaagtggtgt gtttcgatga attcaaatcg gtgaaaaacg
     5701 tctcgggagc catgagcttt gtcatgatgg acggcaagac ccatcaactg attgatattg
     5761 tggaaaatcg gcaactcaat cctttacgcg actatttctt gcgctaccct agaaaagtcc
     5821 gtgaacaagt acgtctagtg gtttcagatt tctatacacc ctatcgcaca ttggtgaaag
     5881 agtgttttcc gaatgcccga atcgtcgccg atcgctttca tattagccaa cacatcggtc
     5941 gggcctttac caaccaccga attcaagtaa tgaagacctt taaaaaaggg gatcgtcggt
     6001 cgaagcactt aaagaaatac tggagactac ttcaaaagaa tgcgtgggaa ttgaatggtc
     6061 agcatcgcta ctggcgtccg tcatttcgag atcatctgac agaagcggag atagtcgatc
     6121 ggctattata ttatgatgac tccttaaagc gaggctacga agtctaccag gtttttcttt
     6181 ccgctatcag aagacaagat gttcctgaat tcgtcgcgct cctaaaagaa gactacaagg
     6241 agttgccaga gcactatcaa ccagtgttca cgaccttcaa gaaatatcga acagaaatca
     6301 aacgagcgtt gcgagttccc tattccaacg gccctataga atgtttgaac aaccatatca
     6361 aagtactaaa acgaattgcc tatggcttta gaaactttca aaactaccga gaacgaatct
     6421 ttttatatcg aggaaaatat ttcaagaaaa ctaagaa
//
LOCUS       DAYYCY010000136         6437 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000136 DAYYCY010000000
VERSION     DAYYCY010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6437
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.136"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..738)
                     /locus_tag="ACHAZB_002217"
     CDS             complement(<1..738)
                     /locus_tag="ACHAZB_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8614850.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNRT
                     VKTTTIQLLKTENNNALRKFDIFNSSIE"
     gene            complement(749..1417)
                     /locus_tag="ACHAZB_002218"
     CDS             complement(749..1417)
                     /locus_tag="ACHAZB_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFM8614851.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(1547..2131)
                     /locus_tag="ACHAZB_002219"
     CDS             complement(1547..2131)
                     /locus_tag="ACHAZB_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8614852.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(2462..2695)
                     /locus_tag="ACHAZB_002220"
     CDS             complement(2462..2695)
                     /locus_tag="ACHAZB_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFM8614853.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(2710..3660)
                     /locus_tag="ACHAZB_002221"
     CDS             complement(2710..3660)
                     /locus_tag="ACHAZB_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein"
                     /protein_id="HFM8614854.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(3657..4499)
                     /locus_tag="ACHAZB_002222"
     CDS             complement(3657..4499)
                     /locus_tag="ACHAZB_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease subunit"
                     /protein_id="HFM8614855.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(4501..5220)
                     /locus_tag="ACHAZB_002223"
     CDS             complement(4501..5220)
                     /locus_tag="ACHAZB_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFM8614856.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
     gene            5768..6070
                     /locus_tag="ACHAZB_002224"
     CDS             5768..6070
                     /locus_tag="ACHAZB_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003013637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8614857.1"
                     /translation="MTIAANNDTSVRLAVNWGEVKFLCAYPKQFKNFITTILGDDFIE
                     SRVYLNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKDFMNTFKIT"
     gene            6142..>6437
                     /locus_tag="ACHAZB_002225"
     CDS             6142..>6437
                     /locus_tag="ACHAZB_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFM8614858.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSD"
BASE COUNT         2246 a         1108 c          842 g         2241 t
ORIGIN      
        1 ttcaatcgaa ctattaaaaa tgtcaaattt ccttaatgcg ttattgtttt cagtttttaa
       61 caactgaata gttgtagttt ttacagttct atttttaaca aatagaactt gatcaattaa
      121 ttctttcaaa tagtaaaaac gatgttcagc tataacaata gtaaatcctt tattttttaa
      181 atcacaaagt aagtcagcaa gtttcttagt cgctttgaag tccaaatttg aagacggttc
      241 atccaatata attaatttag agtttagcga taaactacat ccaatagaaa ttttctgccg
      301 ttcaccactt gatagcgaaa aaatgttttt atctaatata tttgaaagat ctaataattc
      361 agctaattca ttgattcttt tatccatatg ctcttttgta aatccaaagt tttccatcga
      421 aaatactaac tcactcgttg tatttgttgt aaagaattgt cctctaggat cttgacttac
      481 gtagccaata ttttttgata tactttcttg tgttaaggat tgtagcgagt tattttcaaa
      541 taaaatttcc ccaaccaatt ttccgtcata aagtcttgga ataagtccat tcattaactt
      601 taatagtgtg gatttcccac tcccagaaga accagtaatc aatgtaagac tgcctttttt
      661 tattgaaaaa ttaaggtcaa caagcgcttg ttccttaaca tccttatatg aaaaagaaac
      721 attgttaaat tcaaacaatt ctttcacctc aaatcaaaat acttaagaaa aaaatagtaa
      781 cggaaaacag gatgatatag tctaataagc tcatgtttgt tttatagtaa gaacttcttt
      841 tcacaggaga ttctatcccc tttgttattg cattagcggt catttcttca gcaatcatag
      901 ttgcacgtat aagaacggga actaacagaa attccaaata ttgcagaggc tgaaaaaaag
      961 acaagcgaat gtttctcatt tttaaagatt ctttaatcat tcttatttca gaacgataag
     1021 tcggaaagaa tctgatcact actgtaatac ttaacattat ttttttcggt aaatgtattt
     1081 tttctaaaga ggataataat tcattgctag tatagacact aaccaacaac catgagatca
     1141 ttacgaccgg caacattcga aatacaatat ataaggatgt cgcaaaaaat atagtaaaaa
     1201 actctatctt gatagcatca ataattttca ttactaaaaa cagaactgaa taaatcaaga
     1261 tgaaataaat acatgtcttt attttacgaa acatcaatac taaaactgta gcaataatga
     1321 aacaccccac ttgaaatata tttccctttc ctaaaaaaat aatgaccgga aaaaaaatat
     1381 ttaaagtaat caaggttaac ggattgattt ttgacactct ttcacctcca aaaaataaga
     1441 tcgcactgtt atcttaagct gataaaaatg caccgttatt cagataacag tgcgatctta
     1501 tcaaaaagca taactgagaa caattctcaa tgcaagcttt ttttaattaa ctcagtaatt
     1561 caatccaccc aggaacagaa aattgataca atcttctggt taatagcata gtcttttctt
     1621 ttgaatagct atgcttcaat atatcataga atgtagcgta gaatgagtaa atgtagaagt
     1681 ggatttcttc ttcattaaag actaaatttt gattgatcat tccgatagtc tttaacttat
     1741 ctagacaaca gactgtcttt ttcgtcacaa atgatgtaat tttttcaaca atatcttgat
     1801 atttggttcc agctgattgg aataaaatca aatcgaatat acgtttattc tcaaaaatat
     1861 aattaataaa tggatctaat tcattgaagt tctctctcca acttttctct atatctatat
     1921 tttctaagtt taagaggtat ttggtaaact cttcattata catattaata agcgcattaa
     1981 tatgcggctg aataatttct tcaaataacg cttccttaga agtaaaatgt ttataaaaag
     2041 cacccgtagt tacgcctgcg gtcaggcaca gttgccttat cttagtttgc tgaaatcctt
     2101 ctttttcaaa atggcttagt ccactttcta ttaacttttg gtgagttata atataatctc
     2161 tggtttctat gggtacttcc tcctaataag atgaattaac tttattatct tacacaggac
     2221 ttgaagtttc aatcgttaac gtttattact gaactcattc cttagagcta aactaatgag
     2281 aaagagcaga ataactgata caaaaagcaa tcctacaaga attaatagac tcatctaaat
     2341 cattcacctt ttctaaaata atccgagaaa gatttaaaaa aattatatcc agctattagg
     2401 ataagtccta tcataatcac tccaatagcc attttcttat cctccctaaa aataaagtct
     2461 tttacttaaa accttttttg taatcgtcaa taaatttttt ccctttatca gttaattgaa
     2521 atcctatatc taaatagtcc tcatatctaa gtttagccag catctcactt acagatgctg
     2581 gactaacact tagaactttt gctaaatcat tattttttaa tttctttttt ctttttgata
     2641 attcctgaat tgctagtaaa taaattagta tagtagtcgt tttaatttgt accataccta
     2701 ccctccaaat tatttcgaaa ggccttcagc aattttatcc aggttccatt tcatcatcgc
     2761 atagtaacta tcaccatctt gtcctttttc tgcaattgaa tcagtaaaaa tcgttgaata
     2821 gataggaata ttggtatctt ttgatactgt tttcattggt ctatcatcca cactgctttc
     2881 tacgaataag gagggaactt ttgttgtgcg taatttttca actaagtgtt ttatttgatc
     2941 tggtgttcct tcttcttcag tgttgatttc ccaaatgtaa gcagaaggca cattatacgc
     3001 tttcgagaaa tatttaaagc atccttcact tgtaacaatc atttttttat cattcggaat
     3061 tgaagcaaat ttagatttag cttctttgtc tagagaatcc aatttttcaa tatacttatc
     3121 tagattttct ttatagaatt ttttattatc aggatctttt tccgctaact gtttttcaat
     3181 atttttagcg taaataatac cgttttctaa atttaaccaa gcatgaggat cttccttccc
     3241 tttctcactc tgaccctcta agtaaataac atctatgcca tcacttgctg caaaatagtc
     3301 tttgttttcc tctttgttcg catttttaac taattttgta aaccaagcat ttcctccagt
     3361 ttccaagtta acaccgttat aaaaaatcaa atctgctttt gaagtttttt gaacatcttc
     3421 aggcaaaggt tcatattcgt ggggatcttt cccaataggt acgatactgt gtaaatcaat
     3481 tttatctttt gctatatttt cggtaatatc tgctaaaatc gaattagtaa ctactacttt
     3541 taatttttcg ttcgaagcca ctttatcttt gtctttacta tttattgaac atgccccgaa
     3601 tataaaaaga gcaatcaaaa tccccagact tcccagtact gctttccatt ttctcatcat
     3661 aacgatctct ccttctttct taaaaatttt tgttttggtg agattagaaa actaattaca
     3721 aataagaatg ccgctgttaa aacaatactt gatcctgctg ctatgttaaa actataacca
     3781 ataaacaatc caagcaatga tgaacttgct ccgactgttg cagaaataaa tatcatagtt
     3841 ttcaagctat ttgcatataa atatgctgta gctgctggtg taattaacat tgcaacaatt
     3901 aatatagttc ccacactttg catagctgtt actgctacta aagttaatag aaccatcaat
     3961 agataatgat aaaaactgac attcatccct attgcttttg ccattaatgg atcaaaagaa
     4021 gtgattaata attctttaaa aaatagaact actataacca acactaaaac cgaaacgcca
     4081 attgtaatcc acttatcaat atcttgtaca gctaaaatat ttccaaataa aatatgaaat
     4141 aaatccgttg agctatttgc aactcctata aggataactc ctagtgctaa aaaagaactg
     4201 aaagtaatac caattgctgt atctcctttt attacactat tgctttttat aaaagtaata
     4261 atgatagaag ctaatagacc aaatacaatc gcaccaataa aaaaattaat acctaaaata
     4321 taagaaattg ctactcctgg caaaacagca tgagaaatag catcacccat aagagacatt
     4381 cctcttaaaa taataaaaca acctactgtt ccagaaacaa tccctataac tacagatgta
     4441 attagagcat tctgtaaaaa gtgaaagtca tacagaccat taataaaatt ttgaagcata
     4501 ttattccccc tctccaataa atatagtatc tccataggct ttttttaaat tttcttcatt
     4561 aaatacagat tctacacttc cgtgagcaat tagatttcga ttcaaaagaa taatattatc
     4621 aaaatacttc tttaccttgc ttaaatcatg atgcacaatt agcactgttt ttccctgatg
     4681 tttcaatttt tttaacgtat ccataattat tcgttcacta actaagtcta tccctacaaa
     4741 tggttcatct aaaaatatga aatccgcatt ttgagcaaga catcttgcta aaagtacacg
     4801 ttgaaattgt cctccagata attctcctat ttgatgatta gaatattttc ccatatcgac
     4861 cttttccaaa gcctttgaaa ctctttgcca ttctgcattt ttaattcttt gaaaaacttt
     4921 cattccggca tatgttccca tagatacaca ttcttttact tttattggaa aagtaaaatc
     4981 aatatcagct ttttgctcta catatgcaat cttcaccagt tttttattga tatcttcatt
     5041 attaatggag acctctccct gatgagggat aatatttaac attgctttaa gcaatgtgga
     5101 ttttccagca ccatttggac caataatacc tgttaaagca ccttcttgta tttttaaaga
     5161 aatctcgttc aatgctaaaa aatcgttata acaaacatta agctttttca cttctaacat
     5221 aactttctcc ttctaaatga taatgattat cacgatcata tggtatacaa aaattatatt
     5281 ttagtcaacc ctaaatttta tagtttagat ttttttctgt tgctgaagat tagaatgaca
     5341 aaactatttg aaactacgcg tatagtaagt agctttcaaa atatataaaa aaggagcgtt
     5401 ctcaaatgag aacatgctcc taaaactact ttattggcgt atggaaaaat tatacttgaa
     5461 gtggatagcg ggttgaataa gttgataagc atcaaaacat ttataaaaat tattcaagac
     5521 tatgtctgtt ccatatacaa atatgaaaat cagtaaactc catttaactt ttttcgtagt
     5581 gtgtcttttt atacatgttt atcgttatta atagtgaata ggaaaaaaac tatttttgat
     5641 cattgacaat taaatacaat ttttgaataa cgatccctgc tattcgagct agaaactcaa
     5701 gtacgccatg acctgattag cgacaaatac ttagagattt ctttttgttc tgggaaaaca
     5761 aaatgccatg acaatagcag caaataatga tacttccgta aggctcgccg taaactgggg
     5821 agaggtgaaa ttcctatgtg cttacccaaa gcaattcaaa aatttcataa ctactatttt
     5881 aggagatgat tttatagaat ccagagtcta cctgaataaa aaacaaacct gtgcatttct
     5941 aggaatcgat aataatactc tagataagtg gattcgtata tataatttcc cagtaataag
     6001 agttggtggt gtagttcgct ttgacgtaga agatattaaa gactttatga atacatttaa
     6061 aataacttga aattttcttc tcgccgcata tactaaaatt agtgaaatat actgccttac
     6121 tttttttaag gagtgaggat tttgcctaca aagctttcaa atgggaaata taaaactaat
     6181 ctgagatatc caaaaaggtt taaagaaata acaggaattg catcagaaaa gtatcaaaaa
     6241 acatttccaa ataggcaatt ggcgattaaa gccgaaaatg atatgaagaa gaaaatagaa
     6301 aaagttttga gagaggagaa tgcaaactca ttagaattaa aaggaaaaat aacgtttaaa
     6361 aaattttacg aatctaaatg gcttcctaga tatgaattag gtcaaacaat tagatcaaac
     6421 aggcctccta gtgatat
//
LOCUS       DAYYCY010000137         6337 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000137 DAYYCY010000000
VERSION     DAYYCY010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6337
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.137"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(103..807)
                     /locus_tag="ACHAZB_002226"
     CDS             complement(103..807)
                     /locus_tag="ACHAZB_002226"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFM8614859.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(830..1456)
                     /locus_tag="ACHAZB_002227"
     CDS             complement(830..1456)
                     /locus_tag="ACHAZB_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFM8614860.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            1782..4157
                     /locus_tag="ACHAZB_002228"
     CDS             1782..4157
                     /locus_tag="ACHAZB_002228"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFM8614861.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(4181..4672)
                     /locus_tag="ACHAZB_002229"
     CDS             complement(4181..4672)
                     /locus_tag="ACHAZB_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HFM8614862.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            4859..5779
                     /locus_tag="ACHAZB_002230"
     CDS             4859..5779
                     /locus_tag="ACHAZB_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM8614863.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            5933..6277
                     /locus_tag="ACHAZB_002231"
     CDS             5933..6277
                     /locus_tag="ACHAZB_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HFM8614864.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
BASE COUNT         2039 a         1132 c         1248 g         1918 t
ORIGIN      
        1 tgagaaataa agcagataag tacacgaaga gaaaaaagaa gacaaagccc tcgttcagac
       61 tttgcctttt atgattctct ggtaaaaatt aaaatatccc cattatagaa acgagtcgcg
      121 actatgtgtt ttcccgttat cgatataaga cgtatcattg atgccgtgga tagaaaatcg
      181 accgtttgca taagtgacct ttgttacgct gccatttaaa agcccagcac gaacaggctg
      241 tttaggatcg atcagattaa gcaaaaaggc gatcgtcaaa ccatgagata cgactaaaac
      301 atttccacca ccattttttt cgcagtgatg ggcgatatct tcaaatcctg tccatacacg
      361 gtctcgcaat gtttcataag tttccgccca cccagcggta tctaagttgt atacagcatt
      421 agcaatttgt tcaaaagaaa cttctgtggt aaacatttca tcatatgttt taaaattcaa
      481 tacacgaggg atcacacccc acatttccat gtcatatcca ccttcaagag acccgaaaca
      541 ccattctcta atcctttttt caataaaata aggaatttct tttcccttag aatgttctcc
      601 tagaataata cgggcagttt cgattgcacg accactgtca ctagaataag caaatgaaaa
      661 gtcgacattt tttaacccgc gacctaaata atgaatgcct tcttgtcctt gctttgtgag
      721 gggtgtatca caccagcctt gtactcgttc aattgtattg aacatcgttt ttccgtgtcg
      781 tacgatataa agtgtagttt ctgtcatttt attctcctcc taagtcgttt taacgattga
      841 aaaaaggatt tgtccttttt tcctgttcaa tcgtcgttgc atcgccatgt cctggataga
      901 ctgcaaattc atcaggtaat acaaacagct gggtccgaat actatgaagt aattgttcta
      961 aattccctgt atacagatcg gttctaccga tacttcctct aaacagggcg tcgccagaaa
     1021 cgacaaaatc atcaaaaata aagctgacgc ttcctataga atgccctggg gtagggacga
     1081 cgcgaaaaga catgtttcct aaagtataag ttttcatttc aaattcatat tctgcaggcg
     1141 agacaataac atcagcaata tcatcatgtc taccgagtcc agaaagattc aaaatcggat
     1201 cacctagcca tttttgctct agtgggctga cataaactgg gatttgataa tacgcgcgta
     1261 aaggttccac tgcaccgata tgatcgtagt gagtatgggt aagcaaaatc gctaccggct
     1321 gttggtttgt ttttttgatt tcttgtttga ttcgttccgc atcttctcca gggtcaatga
     1381 tcaaaagtgc ttcttcgttg tagaccaaat aacagttttc ttcaatcatt cctgttttta
     1441 atctttcgat atgcatgtgc attcctcctt gatagccaaa acttctttca tccattagta
     1501 taacgcattc attgctagga aacaaaaaca ggtacttatt tctctttact tatctgaatt
     1561 atcagactat atgaaaagta tttggatatt ctaaagagaa ggaaagtaag ttttcatagc
     1621 attttaatat gtccttgtta cattagattc atccaaaaat ccaaattcta tatcctatat
     1681 ccctgcctaa tcagcagggt gtttttttat gtttggaaag agagctcttt ttgctaaact
     1741 aggttttttc aggtaatctt agagagaaag aggtggtgga aatgatcaaa cagtcaaaaa
     1801 tatctgtgcg gcacttagta gaatttattt tgcgacgtgg aagtatcgac aatcgtaaaa
     1861 aaagtaacca tactgcatta gaaggagcga gaatccaccg caagctccaa aaagatgcgg
     1921 gagaaaacta tgaaaaagaa gtctttttaa aaacaacagt tcaattagat gaatattacc
     1981 tgatcgttga aggccgtgca gacggtattt tcaaaagaga cggtatttat tatatagatg
     2041 aaatcaaaac ctccgaacca cgatttgaag atttagaacc agaacaagtg gaacttttct
     2101 ttcatcaagc aagggtgtat gcctatatct atagtcaaga acatgattta caagaaataa
     2161 atctgcaatt gacttacttc cagacggcag aagaagtgat cacgagaaag gttgaacatc
     2221 agacaagaga gcagttagaa acttttttta aaaagctgac tgaagattat aaagaatggc
     2281 ttgtttttca agaaaactgg cgcactgtac gcaatgcctc tttgatggct ttgaaattcc
     2341 cccatgatga gtataggaaa ggtcagcgtg aactagcggt agctgcttat aaaacgatcc
     2401 gtacgaaaca gaaattgttt gtagaagcac ctaccggaac aggaaaaaca atctctacgt
     2461 tattccccgc tttgaaagct gtaggggaag aagagggaga aaagatcttt tatctaacgg
     2521 ctaaaacaat aactagacaa gtagctgaag atgctatgac agcattaaaa gatacaggag
     2581 ctgaagtaaa aagtgtcact ttgacggcga aagacaagat ctgcttttta gatgagacga
     2641 cctgtaatcc agatcagtgc ccatatgcca atggttatta caatcggatt aatgaaggtt
     2701 tatgggatct tttgaatcat gagaatcaga tcactcgtga agtcatcgaa acttatgcaa
     2761 gaaagcatac actttgtcct ttcgaactat cattagatgt cagtatctgg tgtgacgtaa
     2821 tcattgggga ttacaattat ttatttgatc caactgttta tttaagacgt ttttttgaag
     2881 atgaaaaaaa cgaggactat ctgtttttga ttgacgaagc acataatttg gtgaatcgtt
     2941 cccgagagat gtattcggca gaactatctt atgaaaaaac gaaacgaagt aaggaagcta
     3001 taccaaagga attcaaaaaa cttcatcgcc gtttcaacaa actattaaaa gaatttgatt
     3061 cgatccgaga aatagcaaaa gaagatcatt gggattatca tcatcaaaaa gcaccggctg
     3121 aatctttagt aaaggcaggt tatcaattaa gcgaaaaaat taaagaatgg ctggcagaat
     3181 ttcctgagca cccatcacaa gaacaattgc ttccttatta ttttgatttg cttcattttt
     3241 tgaaggtcag tgaattttat gatgatcact acgaaacaac agtcgaaaaa acgtatcgcg
     3301 atctgatagt aaaagagttt tgtattgatc caagcttgtt tcttgagcag tcgttggata
     3361 aaggaaaaag cagtattctt ttttctgcta gtttttcacc actctcttat tatcaggaaa
     3421 cactgggggg acaaaaaagt ctggcttaca aactgccgag tcctttccca gaaggaaacc
     3481 agcaagtttt catcgcaaat tacctagaga cgacttatcg aaaaagggaa cacagtctgg
     3541 caaaattagt agagacgatt catgcttttg ctgatggaaa ggtcgggaat tatttcgttt
     3601 tctttccttc ttatcaatat ttagatcttg cggtggaagc attcagacag caatatcccg
     3661 acagcaatgt gctaatccaa gaaacggaca tgaatgagga agaacgcgaa cgatttttaa
     3721 caaaatttca aacaaatccc gctgaaacat tgattggatt ttgcgtactt ggcgggattt
     3781 tttcagaagg aatcgatttg agaggggacc gattgatcgg cagtatgatc gtaggtgtag
     3841 gtttgccgca gatgaatcat gagcaggaac tgatcaaatc atattatgat gagaaggaac
     3901 accttggatt tgcttacgct tatcagcttc ctggaatgaa caaagtcttg caagctggtg
     3961 gtcgagtgat tcgtggcatg gcggatcaag gaatcgttgt attggcagat caacgatttt
     4021 ctagtttttc ttatcgacgt ttattcccac aacattggca aacagcaaga gaagtacgaa
     4081 gtgttgggga attggaagaa ggaatacagc gtttttggtt atcaaaagaa gcagtaaatc
     4141 aaaaaaacga ggaatgacag acgtcagcct cgttttttga ttaacgatag ttagtgaatt
     4201 gcaactcgat tggtaagtca agtttacgta ataaagcaat cacagcttgt agatcatcac
     4261 ggttcttacc tgtcactcta atctgatctt cttggatttg tgcttttact ttcagttttt
     4321 catttttgat agctgtagtg attttcttcg cgttttcacg atcgattccg ttgaccaaat
     4381 cggcagtttg tcgagctttg ccacctagag cgtgttctga ttcggaaaaa tggatatttt
     4441 tgattggcac atttcttttg atcagcttac caaataatac atctttgatc tgttcgattt
     4501 tgaaatcatc atcgctcaat acaactagat tgctgttctc taatttgatt tctactgtag
     4561 aatccttgaa gtcaaagcga ttggacaatt cttttgttgt ttgctgaatc gcatttttta
     4621 cttcttctgt attgacttct gatacaatgt cgaagctagc atcttttgcc ataggaaccc
     4681 tcctgatata catataaata tttaccatac atctatctta tcataggatt agggcagatt
     4741 cttacctgtt tgctaaaatt cttctcattt gagttcgaag tattttattt taatagataa
     4801 actgttattc ttacatttgg aataccaaag taggggagag gtgcttgtga gtaaaattat
     4861 gactgcgtat cataaaaatg aaagattaaa gaaaatagtg gttattttga taaccggatt
     4921 gaccgctgcg gtcgctttaa attatttttt gattccagcc aatgtctttt ctgcagggat
     4981 gaatgggatc gctcaaatca tttcctcgct tttatatgaa tggtttcata tcaagataga
     5041 cacaggaaca tttatttttc ttttgaatat ccctgtgttt gtattagggt ttctcaaagt
     5101 aggaaaacga gcaacgatcc ttagttttgt gaatgttgta tgtgtatcgg tgatgacgat
     5161 gctgcttcca gtcggacaga tcactgataa tattttgatg aatgccctag taggcggtgt
     5221 tttattaggt cttggcgtag gattatcttt aaaaatgggc tttacaactg gtgggatgga
     5281 tatcatctct cttgtacttt cacaaacaac tggaaaaaca gtcggaaact acatgctgat
     5341 tctaaatggt atcattgtag tcatcgcagg cttcttcttt aactgggaaa gtgccttgta
     5401 tacgattatt tccatttatg caatgactca agtaattgat gccatccaca cgagtcatca
     5461 aaaagtaaca gcaatgatcg ttaccgtcaa gccagaagag gtaacagaag aactagcaaa
     5521 acggatggta cgtggaatga cattactgcc gtctgtcggt ggctattcaa aaaaacaagg
     5581 gaacatgatc atgatggtca tcacccgcta cgaaatgtat gatcttgatc agatcgttca
     5641 tgaaatcgac gacgatgcat ttatcaatat tttaccaact cattcagtat ttggccggtt
     5701 tgcaaatgaa aacgaacaaa aaatgtatcg aacaactggc gtattgccag aaataaaaaa
     5761 tcgttcacaa aaaaaatgag gaatcaggtt tattttagaa taaatttaat ctattacatc
     5821 taataagaac atttttataa atttaaacga gaatcttact tctaaattct catttacaag
     5881 cagctaaaaa aagagtatga taaataaaaa tcgtgttaag gagtgtcgta cgatgagcga
     5941 agcaaaccaa gaacggtctg gacaagaaat agaactaatc aaagaacgta ttcttgctgc
     6001 cttggaaatg gtgattgatc ctgaattagg aatcgacatc gtcaatttag gcttgattta
     6061 tgatatagaa ttcaatcctg aaaatggaga aacagtcatt aagatgactc taacaactat
     6121 gggctgtcca ttagcagata ttttaacaga atctatccat gacgcattaa aagaagtgcc
     6181 agaagtaacg aaagcagaag tcaaactagt ctggtatcca gcatggacga cagataagat
     6241 gagccgttat gcacggattg cgttggggat acgatagaaa tcttggctga ctagctatga
     6301 gaagcaaata ggaaaaacaa aaaagccttt tatcctt
//
LOCUS       DAYYCY010000138         6332 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000138 DAYYCY010000000
VERSION     DAYYCY010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6332
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.138"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..467)
                     /locus_tag="ACHAZB_002232"
     CDS             complement(<1..467)
                     /locus_tag="ACHAZB_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HFM8614865.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANF"
     gene            complement(597..1307)
                     /gene="budA"
                     /locus_tag="ACHAZB_002233"
     CDS             complement(597..1307)
                     /gene="budA"
                     /locus_tag="ACHAZB_002233"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HFM8614866.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNIQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(1320..2984)
                     /gene="alsS"
                     /locus_tag="ACHAZB_002234"
     CDS             complement(1320..2984)
                     /gene="alsS"
                     /locus_tag="ACHAZB_002234"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0034077 - butanediol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HFM8614867.1"
                     /translation="MAEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVEKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAKLTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(3192..3854)
                     /locus_tag="ACHAZB_002235"
     CDS             complement(3192..3854)
                     /locus_tag="ACHAZB_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFM8614868.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKENSFSPVRLFIKMFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSTNCWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(3864..4679)
                     /locus_tag="ACHAZB_002236"
     CDS             complement(3864..4679)
                     /locus_tag="ACHAZB_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8614869.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTDTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(4940..5383)
                     /locus_tag="ACHAZB_002237"
     CDS             complement(4940..5383)
                     /locus_tag="ACHAZB_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HFM8614870.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     HSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(5517..5855)
                     /locus_tag="ACHAZB_002238"
                     /pseudo
     CDS             complement(5517..5855)
                     /locus_tag="ACHAZB_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(5843..6220)
                     /locus_tag="ACHAZB_002239"
     CDS             complement(5843..6220)
                     /locus_tag="ACHAZB_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM8614871.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
BASE COUNT         1917 a         1352 c          997 g         2066 t
ORIGIN      
        1 ttaaaatttg ctaataaaag atctacatat ttttctagaa atgcttgatc gatttcatca
       61 taccctttcg ttactccgca atcgatatct aaaacaccga ttaaattacc ttcttttacc
      121 attggcacta cgatttctga ttgtgcggct gaatcgcagg aaatgtaatt tgcatgggtt
      181 ttgacatcat caacgatcaa tgtttcttgt ttttcagctg attctccaca aactcctttt
      241 cccatcttga tacgtgtgca agaaactcgc ccttggaacg gtcctaaaat caattcatta
      301 ccatcgaata aataatatcc tgcaaagaca gtctcaggca aagtctcatt caacaatgca
      361 gatgaatttg ctaaatttgc taaccagtta tcttctgctt ctaataatcc ttgttgctgt
      421 aataataaaa tttcgtatgc atcttttttc tgtttttctg tccacatgat aaaaaccgct
      481 cctttttgat ctatggagaa gattatagct cagttttttt gtaaacgcta ggtttcctcc
      541 tcaaaaaaac gagatacgtc aggttaatca ccttgaacgc atctcgctgt ctttttctat
      601 tctgcttgtt ggatttctgc tgctaaatca gcataatcaa tcgtttcgtg aacaaaagtc
      661 ggatcatcaa ctggaaaatg ctgtacaagg gacgagatgc ggctaatctc aacttttcct
      721 tcagccactt caaaatccat tacatgtccg ccaaatgtat gtgtatcatc gataaaatgc
      781 aggtgaaatc ccgctgcagc aactccttgg aataattcag gcgtaaagaa cccgactaat
      841 gttccttgga tattgctttg ttggaattct ggttgagact ctgaaatttt tgctagtctt
      901 ctgtaaggtt tttcttgctt cggcatgata cggatgtgca tatttttgaa taatccagtt
      961 atcttcactg ccaaaaatag gttttccccg gcttctctta ctaatatgtc ttttttcaat
     1021 tgttctccat ctgcagtttt tgagacagaa aaactagtat ctggcgtaaa atctgtaatt
     1081 gctgcatagg gggtaagttc acttccatct aattgggcaa ctgagccatc tgatcttcct
     1141 tgataagcta cgccatctaa aaagatgact tcaccatcaa gcccatgcaa tgttcccaat
     1201 cccagagttc ctttttctaa tatatgagca atcgtttctg ttccatccat caagccggcc
     1261 atcaaagctc ctaatgtccc gtgctggtat aagatttttt cagtcatgtc gacacttcct
     1321 taatagaatt gatctggtaa gattgtttct cccaatttct cattatcacg gtaatcaatc
     1381 ggaatatcaa taattacggg tccttctgtc gcaaatcctt cttctaaagc tttttctaat
     1441 tcctctacac ttgtagctcg gattccttta gcgccaaaag cttctgcata tttcacaaaa
     1501 tcaactggac caaagtctac acctgacgcc cgctgatatt tcattttttc ttggaactct
     1561 accatattgt agtgtccatc attccaaatc aaatgaacga tattcaattt tttacgtacc
     1621 gccgtttcta aatcctgggc agagaataaa aatcccccat cgccggaaac tgatacgatt
     1681 tgtgtatttg gacgcaccaa tgcagcagaa atcgcccatg gcaatgcaac accaagcgtt
     1741 tgcataccat tactgaataa caaatgacga ggttcataac ttctaaagtg gcgtgccatc
     1801 caaatataat gactacctac atctacggta acagtcatat catctgttac ttttgactga
     1861 agcgtgttaa taacttctaa aggatgcaag atacctgctt ctccttttga ttgaacaatg
     1921 tcccgctccg ttaattttgc ttgaagagat gctaaatatt cttttgcatc ttctgacact
     1981 tgttgaggct ctaatgaacc tgtcaataaa tcaagagtgg ctgagatatc accgatcaat
     2041 tctctttcag gctgcataaa aggatcgatt tcagctggcg cttcatcgat cacaatgatc
     2101 cgcgcatctt tttccgcatt ccagtttctt gcttcatatt cgattggatc atagccaatt
     2161 gcaataacta gatcacttcg tttcaaaagc atatctcctg gttggtttct aaataaaccc
     2221 acacgaccaa agaaatgcgc ttctaattca cgtgagatca ctccagctgc ttggaatgtc
     2281 tctacaacag gcaatgctgt cttttcaact aattgcctga tggctaacgt ttctttttca
     2341 cttgatcctc gcatacctac taatagtacc ggtagttttg cttctctaat gcgttcagct
     2401 aggtaacgga tatcatctgc agaagcagaa cctaatttag gatcgctcaa tggcttgatg
     2461 acattacctt ggacaggtgc atccactaca tcttgcggga tactgataaa gctcgcgcct
     2521 ttttttgaac tcttagccat acgataagca ttggcaatca cttctgaaat tgtttctgga
     2581 tcttggattt ctgcactgta ttttgtgatt ggctggaaca gtgctgcatt atccatgctt
     2641 tgatgggtca acttcaatag atcgcttcgt tttacttgtc cggcaattgc taaaactggg
     2701 tcaccttctg ctgtcgctgt taccaaacct gtagctaagt tactagcacc aggaccactt
     2761 gttgcgataa ctactcctgg ctctcctgta atacgtccga ttgcttgtgc cataaatgca
     2821 gcattttgtt cgtgacgagt cacgattaat tcaggacctt gatcctctaa ttcattaaat
     2881 acaccatcga tttttgcacc tggtataccg aatacataat caacatgatg attaatcaaa
     2941 ctttctacta cagatttcga accttgtttc attttttcag ccattgaaca tcgtcctttt
     3001 tatgctttat ttttaattat tagttagaaa taatcacaaa taagtgatac agtttctaaa
     3061 cctgtttcat acaaaagtat agcacgtttt atttcatttg tttagccata tgtttcaaaa
     3121 aatgaaaaag aatgacagtt tttacataaa aaaaactgtc attcttaatt gaaagtttca
     3181 atttataaag tctacagatc catagttctc atcaatttac gattgttgaa gtaaatcaaa
     3241 ccaaatacga tagaaggacc tagtagaata caccattggg gctttacgaa aaagaagaga
     3301 aacaaacttg tcaatagtaa aaaatctagt aaactcacat accatttttt caacgtatag
     3361 tagaacgcta tccgtagaac ttgatttggt ttcgacgctg gatttctcac ctgaaaatac
     3421 cagcagttgg tgctaaagga aactgataaa caagcaagta aaacaaacaa cggaatcaac
     3481 cattttccat aagaaaaacg tataaaaaag aaacaatcaa aaatcattat cacactaact
     3541 atccaagcaa atagccaata aaaaaatccc tttagccaaa attggcgaaa catctttatg
     3601 aacaaacgta ctggtgaaaa actattttct tttgctaatt cttctgtcat acctatcatc
     3661 acgatgcaac ctggaccaat aaaaagtaaa gaaaaaataa aggaaaagac attccttcca
     3721 tctatcgaca aaaggaacgc agctgctata tacaagatat tgacacacca aaaacaaagg
     3781 ctacttgcca atgattgata caaccaataa aagaattcca tataaacatt tttgtcaaaa
     3841 cgctgctctt tcataatcac ctcctattcc ttcattcctg atgaagcgat cccttctaca
     3901 aaatattttt gcatcgctaa aaatataaaa gcaacaggaa gaacagaaac aactgaggca
     3961 gccatgatta atgcgtactc tgcatcaaat agcccaataa acattttcaa ccctaactgg
     4021 attgttttat tttccgtaga agacaaataa atgaatggcc ccatataatc gttccatgta
     4081 ttaacgaatg tgatgatcgt caaactggca atcgccggtt ttgtaagagg taaaatgatc
     4141 ttgcgataga ttccccactc tgataagcca tcgatccgcg cactctcaca taaagaatct
     4201 gggatactac tgtaatattg cttcaacaag aacacaccaa aagcattgaa cgcttgtaat
     4261 agcaccaacg accacaacgt atctgttaaa cccacttttc tcatcataat gaactgcggg
     4321 atcatataag actgccaagg gacagcaatc gtgccgatat atgctaaaaa taaaatatcg
     4381 cgccctttaa aatttagttt agaaaacccg taagcagcaa aactagaagt aaacaactga
     4441 atcaacgtga tcacaacgct taaaaaagct gtattcttaa aaaatgtcac taaaggaata
     4501 cgagaccaaa taaccagata attctctaaa tgccaagttt tagggatcca ttgaatcgga
     4561 atcgtgaaca cttcattatt tgctttgaat gaagcagaaa ccatccataa aaaaggaatg
     4621 atcgtcaata aagctaatac aatcagcaga atcataccca accattgaat aaagggcatg
     4681 ttttttgaac gaatttttgc tttttttctt tcatttcgaa cacctccaaa gttataatct
     4741 tcatttcttt gtaatgatta caatccagaa caacggttat tatagctgat tcatttccgt
     4801 tcttcttatt tttgtaagcg cttatttaac aagttatgta aatctcttta tttttttgat
     4861 ttaaaaatga aaaaatacaa aagcaagact gtcatacgtc tcgcttttgt attgaaattt
     4921 cttcgattag agatacttat tactgttcaa aagcttcttt gaaacggtga atcaattcat
     4981 tgactacagg tgataaaatc cgattctttt tccagactag catgcagttc gtttcaatca
     5041 ttgggtcaag cggaacgaat gtcactcgct ttgaatgatg acttacagca ccttctatga
     5101 ctaaagcagc acctacctga ttctcaatta gcgcaaatac attgaaaatc aaattgtacg
     5161 ttcctgtgat cagcaactgc tcctttttta tgcctaacca gtcagcaaac attcgctgta
     5221 tttctggacg ctttgagatc aaaagaggta cattcgcaaa gtcttctttt gttatcacgt
     5281 ctttttttgc taaaacagaa ttttctaaaa tgagcaatcc ccatttttcc tttcttggta
     5341 acaaaatcgt ttcgtatttc tctgtatcta ctggatctag caaaatcgct aaatctaaca
     5401 ggcttttttc tagtcactcc gtgatatcat cacttgttcc gctgtggata tgaaattgaa
     5461 cgctgggata atcacggatc aacttttcta gtaatagtgc caatgtattc gaactatcag
     5521 cttccacaca gccgatcgta aatcggccgc tgaataattg tttttttctg ttctcgaatt
     5581 cttgagtggt ttgattcgtt aaatctaaaa tttcttctgc tcggcttttt tagaaaataa
     5641 cctgcttctg tcaagatcat tttgcggttt tcgcgtacaa acagttctgt tcctagttac
     5701 tcttctaact ccttcagttg tcggcttaag gttggttgtg taagatgtag aacttctgcg
     5761 gcttgagaga ttgttccttc ttctgcaatt gtccaaaaat attttagtaa gcgtaactcc
     5821 atagcgatgc tccttgattt ttttacttga tacattccat tttatttcta gataacacac
     5881 gatcatattt cttgattttt ccttgtaaac gttccaaaca ttcttgcatt tcagcaattt
     5941 ttttcgtcaa aagttcactt tcttcaatca aaagcttacg ccgctcaaat acagttgctt
     6001 ctccttcgct agataggcga atatattcaa tcaaagactc gatcgataat ccggcatttc
     6061 gcatacagat cgtaaagtcg acccatttta ggtcttcttc tgtataatca cggattccgc
     6121 cttttgtccg attaactggt ggaatcaacc cgattcgttc ataatagcgc aatgtatcgg
     6181 ccgaaatgcc cttttcttca ctgacttgtt tgatattcat ctgatcactc tttctcttaa
     6241 tttcttgcca ctttacttat gtattttatt tattctacaa ctttaagtgt actccaagtc
     6301 aatactcatt tctattaaaa ctaagctgga tt
//
LOCUS       DAYYCY010000139         6257 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000139 DAYYCY010000000
VERSION     DAYYCY010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6257
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.140"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..168
                     /gene="mobC"
                     /locus_tag="ACHAZB_002240"
     CDS             <1..168
                     /gene="mobC"
                     /locus_tag="ACHAZB_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HFM8614872.1"
                     /translation="QAIARDLAKAGGNINQIAKWCNTHQHDVAPDDAQRLSENLKIMQ
                     KELQKIWQQLK"
     gene            150..1064
                     /locus_tag="ACHAZB_002241"
     CDS             150..1064
                     /locus_tag="ACHAZB_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFM8614873.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     TLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPNHQIAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKTEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYTQFCDSLKTFGIHCLNRGKNFTYELVHKKK
                     KVRSNKLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTFTP
                     EQSEIRPTRRNHEQHVERGTDHQQELSL"
     gene            1309..1998
                     /locus_tag="ACHAZB_002242"
     CDS             1309..1998
                     /locus_tag="ACHAZB_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8614874.1"
                     /translation="MAFYLPYLLISASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLV
                     GVIFALSIVLSGLFGVLEGNKRVSRSILLGDITQKYEAACDKKKKEQALAQKMEEFTA
                     CYEDMNNIFVDQEKRLTDKNMKKLTRFYQNLPEKQQKEVQEKYEQVKKDVQYFKDTQT
                     EEACCNLFSDTIPFSTSEQERKERQQSVTYERYETLLQQATNIQNPTKKETALNYLES
                     VKEWLDQQQQN"
     gene            complement(2119..2205)
                     /locus_tag="ACHAZB_002243"
     CDS             complement(2119..2205)
                     /locus_tag="ACHAZB_002243"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFM8614875.1"
                     /translation="MFERFICPLLVEVCVSLFSYWLNNRNKK"
     gene            2881..3831
                     /locus_tag="ACHAZB_002244"
     CDS             2881..3831
                     /locus_tag="ACHAZB_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFM8614876.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     LTFDQLKDLSNYKATANNRFINDLEMTYDKLMDLRFGKRSANGLDIDRFVMFSEFKIR
                     GTAKDPYVDIQVYEKALPLLNNLDEWVRYSLQQFNELQSSYAKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYWNNQANVDNRVIKPIRQELTAIFKGLSIKKKYGKGRGKPVV
                     GYQFTFKPEIKKADDFNKHAPTKEEQNKALALADKQKEYEEPATTINEKIKQKKRELE
                     LLQQIKQLEKEKEALEKQTESALSKKEKKSLLSDLDNLFQ"
     gene            3852..4385
                     /locus_tag="ACHAZB_002245"
     CDS             3852..4385
                     /locus_tag="ACHAZB_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFM8614877.1"
                     /translation="MQPQETFTIKELSELFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLGNQMLLDRLHEYEQDNDKNVNIPSKNVVTNKLLDQLREENEFLRE
                     QLALREQNNSHIQKLLDQQQQLTLQANKQIEKLQEKLQLTYTEEAPENDSTTLSEATE
                     TIDKQIDTEKKWWQLWK"
     gene            4401..4571
                     /locus_tag="ACHAZB_002246"
     CDS             4401..4571
                     /locus_tag="ACHAZB_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8614878.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNS
                     PNEELFVKETDK"
     gene            5021..5554
                     /locus_tag="ACHAZB_002247"
     CDS             5021..5554
                     /locus_tag="ACHAZB_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8614879.1"
                     /translation="MKKFTLGVALLTIGAVVLPSTTIVYASENSQVVSLNENNATENV
                     VIEYTEDGMVVTKYEDGYSAEDFANSSDIMTRAAVARWGKKEYTNIAVTTGVAANAIN
                     AAFWAGGKASTSMFGIPGWAIGALLTGASWTKKGASPGNAVAKEWDKNGNGWIGFYYQ
                     RGYDAAGRVVATRYSTL"
     gene            5566..5769
                     /locus_tag="ACHAZB_002248"
     CDS             5566..5769
                     /locus_tag="ACHAZB_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8614880.1"
                     /translation="MFLISKSLKSLKTFTKKNPQIATIRIFIFAIAVAIIVNMLSGGQ
                     LYKSGLFLVLILVVLDIIKSKKK"
     gene            6046..>6257
                     /locus_tag="ACHAZB_002249"
     CDS             6046..>6257
                     /locus_tag="ACHAZB_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HFM8614881.1"
                     /translation="MANDTSVGRTERVGLMSEQHQNLASDHSKKYTYRSEPKQISFRV
                     SESEFAKLKQSAEALQMSVPAFVKAKA"
BASE COUNT         2310 a         1088 c         1161 g         1698 t
ORIGIN      
        1 caggcaatcg cgcgcgattt agccaaagct ggagggaata tcaatcaaat tgccaagtgg
       61 tgcaatacac accaacacga cgttgctcct gatgatgctc aacgtttatc cgaaaaccta
      121 aaaatcatgc aaaaggagct acaaaagata tggcaacagt taaagtaagt cgaaccactt
      181 catgtagccg agcaatcaat tacgcagaac cacgagcaac cgtcaaaaca ggaataaact
      241 gtgacattga ttatgccaaa agtgaaatga aacaaatacg cacgctttat ggaaaagacg
      301 atcgtgtaca agcacactta ttgattcagt cctttcgacc aggagaaatt accgctgaaa
      361 aagccaatca actaggggca gagtatgcga aaaaaatagc ccctaaccac caaatcgcga
      421 tttacacaca tacagacaaa gaccatattc ataatcacat tgtcattaac tccgttaatc
      481 ttgaaactgg gaaaaaattt caagcccatg ggcaagcatt tttaaacaaa tgttatgacc
      541 taaatgacga gatttgtctt gaacatgact taacaatcac ggaacgagga aagaaaaccg
      601 aaaaacgaac catgtctgaa attaaattaa aagaaaaaaa tgaaccggtt tggaaagacg
      661 aaattcggtt tgcgattgat caaacaatga aaaacaaaaa aactcgaacg tatacccaat
      721 tttgtgattc acttaaaaca ttcggcattc attgcttgaa tcgcggaaaa aattttactt
      781 acgaattggt ccacaaaaag aaaaaagtac gatccaacaa actaggaaag gattacgaaa
      841 aggagacgat tttacatgag ctggacagac gagaacgaac ctacaatcaa caacgaacca
      901 acgagctacg aagagctcga gctagcattc gcgaaactgc agaagaagta cgaagagaac
      961 aacgaacttt tacgcctgaa caatcggaaa ttagaccgac tcgaagaaac catgaacaac
     1021 acgttgaacg aggaacagac caccaacaag aattatcgct ctaaaacgtt aaaagacatc
     1081 gaaaacgaaa taaagagaca acgtgccttt cttgcgcctt atacccaaga aaggatacaa
     1141 actacgatgg attcacaagt caaaaaactc caccaagcgc tattagacca gcgaaaccaa
     1201 gaacaaaaaa aggctttaat tcaacgatcg atggatattc ttgccccctt atgttgctta
     1261 gtactaacat tattagcctt ttggttggta tggggagaat aaacagacat ggcattctat
     1321 ttaccctatc tactcatttc tgccagtatt ttaggaatta tttggcttag ctataaggct
     1381 tatcaaacaa gatattcatt aaaaggctct aaaattcgat ttaagcgatt tttattggtc
     1441 ggggttatat ttgccttatc aatcgtttta agtggtttat tcggcgtttt agagggtaat
     1501 aaacgtgttt caagaagcat tttattaggg gatatcactc aaaagtatga agcagcatgt
     1561 gataaaaaga aaaaagagca agcattagca caaaaaatgg aggaatttac cgcttgttat
     1621 gaggacatga acaatatttt tgtggatcaa gagaaacgtt taacagataa aaacatgaaa
     1681 aaactcactc gtttttatca aaacttacca gaaaaacagc aaaaagaggt acaagaaaaa
     1741 tatgagcaag taaaaaaaga cgtgcaatat tttaaagata cgcagacaga agaagcttgt
     1801 tgtaacttat ttagcgatac aatccctttt tcaaccagtg aacaagaacg aaaagaacgg
     1861 caacaatcgg tcacatacga acgttatgaa acactgcttc agcaagcaac aaacatacaa
     1921 aatccaacga aaaaagaaac ggcattaaat tatctagaaa gtgtcaagga atggttggat
     1981 cagcaacaac aaaattgaat ttgattagca atcatgttaa actgaacctg caaatgatca
     2041 aacattagat acacgaaagc cccagagagg tcggattctc tggggctttt tttcaggttg
     2101 atgtcgcctt tattgatttt acttcttgtt tcggttattc aaccaataac tgaataaaga
     2161 gacacaaact tctacaagaa gggggcaaat gaaccgttca aacattagat acacctcctt
     2221 ccagtcgcaa gactgtcgga taggcgacaa gttattatac cttacaaaat aaaatctgtg
     2281 tggtgttttt aagtatccct tattttcggg ttgttagggc ttgccctgac cgcctgtaag
     2341 gcgctttaag aacaagataa ggttttttac ccaagactgt cttaaaacga cttagacgag
     2401 ctatcagagg cttcccccac ctccgttact gacttgtttt tagcaaagga gcctttaggc
     2461 gactgcgcag tacaatgtga gagcatagcg atcacacttt tttagcgacc gtagggagca
     2521 aggttttgaa ttggcagttt tgaaaaatgc gcgaacctgt tggtagaaaa aagaagtggt
     2581 ttttaggcgg gaaattttgt caccctcttt tataatctct ttttaaacct ttttttaaac
     2641 ctctttaccg tcggctgtgt ccttactcct tcaatggata cagcgtttta taaactacat
     2701 tttgtctgct tttaaactac attttgtctg cttttaaact acattttgtc tgcttttaaa
     2761 ctacattttg tctgctttta aactcttttt ttgcactcac gtatgttata atatgcatta
     2821 tcacagacaa aacgttgttt ataacgtctg tatccattga aggagtaagg aggaatatct
     2881 ttggcaaatg aaatcgtgaa ataccatcat gaattaaata ccattccgtt gcggaaattt
     2941 acaccagttg aaatgaatct atttttctct attgtgtcta gaatgcgcga aaaaggcgat
     3001 aaaacagtcc gtcttacttt tgaccaacta aaagatttaa gtaattataa agcaactgct
     3061 aataatcgtt tcattaatga tttagaaatg acttatgata aattaatgga tttacgtttt
     3121 ggtaaaagaa gtgctaatgg gctcgatata gatagattcg ttatgtttag tgagtttaaa
     3181 atacgtggaa ctgctaaaga tccatatgta gacatacaag tttacgaaaa agctttacct
     3241 ttactaaaca atttagatga gtgggttcgc tattcactcc aacaatttaa cgaattacaa
     3301 agtagctatg caaagactat gtttcgatta ctaaaacaat atcgaactaa aggttttgct
     3361 tatttttcta aagaggattt tcatgaacta ttagatattc caaaaagtta ctggaacaac
     3421 caagcaaacg ttgataatcg tgttataaag cctatacgac aggaattaac cgcaatcttc
     3481 aaaggattat caatcaaaaa gaaatacgga aaagggcgag gaaaacctgt cgttggctat
     3541 caatttacgt ttaaaccaga aatcaaaaaa gcagatgatt ttaataaaca tgcaccaacc
     3601 aaagaagaac aaaataaagc attggcttta gctgataaac aaaaagaata cgaagaacct
     3661 gctacaacaa ttaacgaaaa aatcaaacag aagaaacgag aacttgaact cctccaacaa
     3721 atcaaacaac tagaaaaaga gaaagaagct cttgaaaaac aaacagagtc cgcactttcg
     3781 aaaaaagaga aaaaaagttt attatctgat ttagataatt tattccagta gcaacctagg
     3841 aggcaaccag tatgcaacca caagaaacct ttacaataaa ggaattgtca gaacttttta
     3901 agatgagtag gcaagcaata tcgaaacata tccaaaaatt agattcttcc atgatagcta
     3961 aaaatgagcg tggttataag gttgtattgc gttctggagt attgcaactt gctcgcaact
     4021 taggcaacca aatgttgcta gataggttgc acgagtacga acaagacaac gataaaaatg
     4081 ttaatatacc aagcaaaaac gtggttacaa acaagttgct agatcagttg cgtgaagaaa
     4141 atgaattttt acgagaacaa cttgccctca gagagcaaaa taattctcat atacaaaaac
     4201 tactcgacca gcaacaacaa ctcaccctac aagccaacaa acaaatcgaa aaactgcaag
     4261 aaaaactaca gttgacttat acagaagaag ctccagagaa tgactcaacg actctttctg
     4321 aagcaacaga aacaatagac aaacaaattg atactgaaaa aaaatggtgg caactttgga
     4381 aataactgga gggaacattc atgacaagaa aaaatcttct attcggttct attttactag
     4441 gaattctagg attcagtccg atgttattgt cagtgatcaa taaagggaat aaacatggtg
     4501 aattgaaatt gaatccaaat tattacaaca gtcctaacga agaattgttt gtaaaagaaa
     4561 cagataaata ataaaaaaga ctgctctttc gagcagccag ttaaaaaata atgagtgttt
     4621 attcttgaaa aagcaacgcg cttatgaagc gctaccaaaa atttatcata ttttattaaa
     4681 aataacaacc cagtaaaaaa tgactggtaa aaattcacaa agaaacggaa agaagcaacc
     4741 gcagaaaatc aagcttaaaa agagcttttg acctagacca atcgaaaatc acaaagtgag
     4801 cgcctgtatc tattgctaca ggcgctactt ggcatagtgt acattatgtc aacaagaatt
     4861 taatataaca tattttccta atgcattcta actataccac attatcaaaa atataaatta
     4921 tctttactaa ataaaatatt gacaagccta taccttttgt tttatgattt tcacaaagaa
     4981 aacgttttct atatattttt ctgtatcagg aggaaattct atgaaaaaat ttactttagg
     5041 ggtagctttg ttaactattg gggcagttgt tttaccgtca actactatag tttatgcttc
     5101 agaaaattcc caagtggtat cattaaacga aaacaatgct actgaaaatg tagttattga
     5161 gtacactgaa gatggtatgg tcgtaactaa atatgaggat ggttatagtg cggaagattt
     5221 tgcaaattct tcagatatca tgacaagagc tgcagttgca agatggggca aaaaggaata
     5281 tacaaatatt gctgtaacca ctggagtagc tgctaatgct attaatgctg ccttttgggc
     5341 aggagggaaa gctagcactt ctatgtttgg tatcccaggt tgggcaattg gtgctttgtt
     5401 aacaggtgca agttggacta aaaaaggtgc ttctccaggg aatgcagttg caaaagaatg
     5461 ggacaaaaat ggtaatggct ggattggatt ttattaccaa aggggatatg atgcagctgg
     5521 aagagttgta gctactagat attcaacgtt ataagttaag gagtgatgtt tttgatatct
     5581 aaatcattaa aatcattaaa aacatttact aaaaagaatc cgcaaatagc tactattcgt
     5641 atttttatat ttgctatagc tgttgctata attgttaata tgctaagcgg agggcaatta
     5701 tacaaatctg gtttattttt agtgttaatc ttagttgtct tagacataat aaagtctaaa
     5761 aaaaaataaa tcgatacatt ttagaagaat agggttgttt gaatattaca aagcaacaat
     5821 ttcaccttcg gtgaaataag cgaaacttta caaaaagtgg ctagcaaatg ctagcctttt
     5881 tttgtattgt ggagcgtgga ttttgagggg gattcaaggg ggagcttgcg ctcctttgac
     5941 aagcggttat ttgcaccaaa tacggaggta tttggctagc aaatatagag cttgccaaac
     6001 ccttttagtg agctttactc attgtacctt tttaggtaca atggtattgc gaacgatacg
     6061 tccgtaggac gtactgaaag ggtgggactt atgagcgaac aacatcaaaa tttggctagc
     6121 gatcattcta aaaaatatac ctatcgatcc gagccaaaac aaatcagttt tcgagtgagc
     6181 gaatccgaat ttgcaaagct gaagcagtca gctgaagctt tgcaaatgag tgtgccggct
     6241 ttcgtgaaag ccaaggc
//
LOCUS       DAYYCY010000140         6228 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.141, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000140 DAYYCY010000000
VERSION     DAYYCY010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6228
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.141"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            106..966
                     /gene="ylqF"
                     /locus_tag="ACHAZB_002250"
     CDS             106..966
                     /gene="ylqF"
                     /locus_tag="ACHAZB_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HFM8614882.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            959..1726
                     /locus_tag="ACHAZB_002251"
     CDS             959..1726
                     /locus_tag="ACHAZB_002251"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFM8614883.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            1782..2639
                     /gene="dprA"
                     /locus_tag="ACHAZB_002252"
     CDS             1782..2639
                     /gene="dprA"
                     /locus_tag="ACHAZB_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFM8614884.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            2761..4839
                     /gene="topA"
                     /locus_tag="ACHAZB_002253"
     CDS             2761..4839
                     /gene="topA"
                     /locus_tag="ACHAZB_002253"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFM8614885.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            4808..6184
                     /gene="trmFO"
                     /locus_tag="ACHAZB_002254"
     CDS             4808..6184
                     /gene="trmFO"
                     /locus_tag="ACHAZB_002254"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047151 - tRNA
                     (uracil(54)-C5)-methyltransferase activity,
                     5,10-methylenetetrahydrofolate-dependent [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HFM8614886.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
BASE COUNT         2163 a         1098 c         1397 g         1570 t
ORIGIN      
        1 ctaatagatg gaatttcttt ataataaaaa agtgaattta tattgaaaaa gaaaaaaaca
       61 agtaaagccg gggaatattc cggtatgaaa ggaatggaga gaacaatgac catccaatgg
      121 tttccgggac atatggcaaa agcccgacga gaagtttctg aaaaaatgaa atatgtggac
      181 atcgtatttg aattggtaga cgcacgatta cctttatctt ctcgtaatcc aatgctagat
      241 cagatcattc agcaaaaacc tcgccttgtt ttattgaata aagcggatct ggcagatcca
      301 caacagacaa agctgtggca agaatatttt cgtaaacaag gccatcttgc attagctatc
      361 acggcacaag aaagtaaagg aattaaaggt ctagtacctg cggctaaaga agcattgaaa
      421 gaaaaacggg caagagatgc tgctcggggg atcaagcctc gcgctattcg ggcaatggtc
      481 ttaggtattc caaatgttgg gaaatctacg ttgatgaatc gactagtagg caagaaaatt
      541 gcacagacag gaaataagcc tggcgtaaca aaaggtcagc agtggcttcg tttaggtaat
      601 gagttggaat tgttagacac accagggatt ttatggccca aatttgaaga tgaagaaatc
      661 ggcaaaaaac tagcgctgac aggcgcgatc aaagaccagc ttgtccattt agatgattta
      721 gcgatttacg gattagactt ttttgcccga tattattcag gaagaatcag cgatcgttat
      781 ggtttatcaa tcgagcaaga agaaaaattg gcaccggaat tactgatgga tattacagca
      841 aaacgtgggt ataaagaaga ctatgatcgt ggcagcgaat tagtcgtgca tgatattcgt
      901 caaggaaaac ttggacgata cacactggat cgttgcgaag aaatggagca aaaaagtgat
      961 gactgaatcc atccaaagta tcaaagaacg attgaaaagc atacagagaa tagatgatcc
     1021 tacactagaa tcattaagaa atgatccacg caaaggcgtg cagcaagcac tggcttcttt
     1081 tgaacgacga ttgaaaaagg aagcattaat taaggaaaaa tatgaagaaa tgttttgctt
     1141 tgaaaagcaa gggtacgcac aaggtcacca atggattgcc ggcattgatg aagtaggaag
     1201 aggaccatta gctggacctg tcgtggcagc tgcagttatc ttgccgaaaa atgagatcat
     1261 attagggctg aatgactcca aacaattatc agagaaaaag cgagaacttc tttatcaaca
     1321 agttcaagaa aaagccctgg caatcgggat cgggatcgtt gatcagcaga caatcgatga
     1381 tgtgaatatt tatgaagcaa gtaaattagc gatgatccaa gcagtagaaa aattgtccat
     1441 tgtaccaagc tatttgttga tcgatgcgat gaccttggat ttgcctattc agcaggaaaa
     1501 aataatcaaa ggggatgcac gatctgtgtc gattgctgct gcaagtatca ttgctaaagt
     1561 atatcgagac caattgatga aagaatatga cgtgctttat cctggatatg gtttctcgca
     1621 taatgcaggt tatggaacaa aggaacactt agaaggactt gaacgacaag gaattacgcc
     1681 aatccatcgt aaaagttttg cacctgtaaa aaaatattta caataaaaaa cagccgcttt
     1741 tacgtatctt attagaaaga gagacgaaag gcggttttat gatgtatcaa atagaagaaa
     1801 atttattgaa acttgcttat acaaaaggaa tcagtagtca aggaaaatgg gcacttgcta
     1861 actggatgat gcagtatcct tataaagaaa tcggatttga agacatcatt aagataggta
     1921 aaataaccaa acatcgggag ctgtttcgca aaagttggca ggaaattcac gctaactggg
     1981 gcaaaatcca atcgaaacag tcgtttatca cttgctttga tcccaaatat ccccctcagc
     2041 tgcttcactt gacctatccg ccaattgtac tgttttataa tggtgatcta tctttacttt
     2101 cttgtaacat gttaagcgtt gttggtggaa gacacacttc tgctttagcc aaaaaaacag
     2161 tttattattt actaaaacca gtgatagaag ctggatatgt gatcgtcagt ggctgcgcga
     2221 aagggataga tacatacggt caccaagcag caatctctca tggtggacgg acaatcgcag
     2281 tcattggaac agggatagag caagtctatc ctgctgaaaa tcgtgacttg caagaggtga
     2341 ttgcaaatga tcatcttctt ttgagtgaat atcctcctca atccggcccg aaaaagcatt
     2401 gttttccaat gagaaatcgg attatcgcag ctttgtctaa aggaacatgt gtctttgaag
     2461 ccaagaaaaa aagcggctct ctgataacag cacagcaagc attggatctt ggaagaacag
     2521 ttttttctgt tccaggaaat attttaacag aacattctac cggatgtcat catttgatcc
     2581 aagacggtgc agtttgcacg gtttcgggtg aagatatctt ggcagaattt aaagaataac
     2641 accatacttt tcatttgctt ttcttgacaa acgttttctg aatggatatg attagctacg
     2701 atttgtatga atcctctttt aatagtagga acacgacaat ggaaaggagt ccattcagta
     2761 atggcgtata aatatttagt gattgtagaa tcacctgcaa aagccaagac aattgagaaa
     2821 tatcttggac gaaactataa agtaatggcc agtgttggcc atataagaga tttaccgaaa
     2881 agtaaaatgg gtatcgattt tgaaaacaat tatgaacccc attatatttc tatacgcgga
     2941 aaaggcgatg tcatcaaaag cctgaaagcc gcagcaaaaa aagctcaaaa agtttacttg
     3001 gcaagtgacc cggatagaga aggagaagcg attgcttggc atttagcgta cctacttggg
     3061 ttggatctga aagaaaaaaa tcgggtggtc ttcaatgaaa tcacaaaaga cgcagtcaaa
     3121 gcagctttta aggaaccaag aacgatcgat gtagatttag tagatgcaca gcaagctcgt
     3181 cgtaccttag acagaatcgt tggttattcg atcagtccta ttctctggcg taaggtcaag
     3241 aaagggttaa gtgcaggacg tgtccaatct gtcgctttaa aaattattat tgaccgtgaa
     3301 aaagagatcc gagaatttgt tccagaagaa tattggagca tcgacggtaa ttttaaaaaa
     3361 gctcgcaaga aattcaaagc aaatttctgg ggaatcgacg gtaagaaaaa gaaattacca
     3421 gatgcacaaa gtgtaaaaga agtcactgct agaattgatg gaacagactt cgaagttaaa
     3481 aaagtagaaa agaaagagcg caaaagaaac cctgcgaatc ctttcactac aagtagcctg
     3541 caacaagaag ctgcccgtaa gttaaacttt cgaacaagaa aaaccatgat ggtagcacag
     3601 caattatatg aaggaatcac tttgggtaaa caaggaacag tcggcttgat tacatacatg
     3661 cgtactgatt ctacccggat tgccgataca gcaaaagcag aggccgcagc atttattgaa
     3721 gatacgtatg gaaaagaatt ttctagacat gatcaccgaa agatcaaaaa tgctcaaggc
     3781 gcacaagatg cccatgaagc tgtgcgtcca acgagtgtgc ttcgtacacc agatgaactc
     3841 aaacaatatt tagataaaga tcaattgaaa ctttatacat tgatctggtc acgttttgtg
     3901 gctagtcaga tgacaccagc tattttagat acaatgaaag tgacattaga acaaaatggt
     3961 gtcatcttca ttgcgaacgg ttcaaaaatc aaattcaaag gattcatgca agtgtatgtg
     4021 gaaggtagag atgacggcaa agaagagaaa gaaaatattc ttccagaact tgaagaaggc
     4081 gatgtagtcc aatctgttga tattgaacca aaacaacatt ttactcaacc accagcacgt
     4141 tttagtgagg ctacgttgat tcgtgcacta gaagaaaacg gggtagggcg accttccact
     4201 tatgcaccaa cacttgatac gatccaaaga cgctactatg taaaattgac acaaaaaaga
     4261 tttgagccga ctgaactagg agaaattgtc aattcattaa tctgtgaatt cttccctcaa
     4321 atcgtcgata tccatttcac agcagaaatg gaaggagatt tggataaaat tgaagaaggc
     4381 acggaagcgt gggtcaaagt ggttgatcgt ttctataaac cgtttgaaaa agaactgacg
     4441 aatgcggaag aaaaaatcga aaaaattcaa atcaaagatg aaccagcagg attcgattgt
     4501 gatgtctgtg gacatccgat ggtgataaaa ttagggaaat atgggaagtt ttatgcttgc
     4561 agtaattttc ctgattgtcg gaatacgaaa ccaatcgtaa aagaaatcgg tgtgacttgc
     4621 ccagtatgtc atgaaggaca agtcattgaa cgaaaatcta agaaaaacag actattctat
     4681 ggttgttccc gtttcccaga atgtgaattc acttcttggg ataaaccagt gggcagaaat
     4741 tgtccaaaat gtgacggata tctggtcgag aagaaagtca aaggcggtaa acaagtagtc
     4801 tgtgtaaatg gagattacga agaagacatc caaaaataat agaaaagagg ttctttcatt
     4861 gaaaatggta aatgttgttg gagcagggct tgccggaagt gaagctgctt ggcagatcgc
     4921 acaagcagga gtccctgtcc gtttatatga aatgcgtcca gtcaaatcga ctgaggcgca
     4981 tcacacaagt aattttgctg aactagtatg ttccaattca ttaagaggaa acagtttggc
     5041 taatgcagta gggttattga aagaagaaat gcgccgattg aattcggtag taatcacttc
     5101 ggcagatgaa acagcagtgc cagctggtgg tgccttagct gttgaccgcg attctttttc
     5161 tgaaacaatt acccgaaaag tcaaagagca tccattagta acagtgataa acgaagaaat
     5221 tactgaaata ccggaaggaa tcaccgtagt agcaacagga cctttgactt ctcatccatt
     5281 agcagaaagc atcaaagcat tcaatggttc agatggtttt tatttctatg atgcagctgc
     5341 accaatcgta gacaaagcta cgatcgatat ggataaagta tatttgaagt cccgctatga
     5401 caaaggagaa gcagcttact tgaactgtcc gatgacagaa gaggaattca aagctttcca
     5461 tgaggcacta atcaatgccg aagtggcaga acttaaatct tttgagaaag aaaaatattt
     5521 tgaaggatgt atgccaattg aagtaatggc acagcgagga ttcaaaacga tgcttttcgg
     5581 acctatgaaa ccagttggat tggaagatcc taaaactggc aaacgtcctt atgctgttat
     5641 ccagctgcgt caagacaatg ctgcagcttc tttatacaac atcgtgggct ttcagactca
     5701 cttgaaatgg ggcgagcaaa aacgtgtttt ccggatgatt cctgggttag aaaatgctga
     5761 attcgttcgc tatggtgtga tgcaccgaaa cagctttatg aattcgccag aattgctgaa
     5821 accgacttac caatcaacga aacgcgaaga tctgttcttt gcaggtcaga tgactggtgt
     5881 agaaggctat gttgaaagtg cagcgagcgg actacttgct ggaatcaacg ccgcacgctt
     5941 agcaaaagaa atggaaccag tcgagtttcc acaagaaaca gctattggaa gtatggctta
     6001 ctacattact catgcggaag gaaaacattt ccaaccgatg aatgccaact atggtctgtt
     6061 tcctgaactg ccagagcgca ttcgagacaa aaaagcaaga tatgaagcaa ttgcacaacg
     6121 agctttaaaa gctaatgagg caataaaaac agaattagaa aaaactgcaa tcccaaatga
     6181 gtaaggaaac tccatgaaga gggtgagacg gatagtctcg ctcttttt
//
LOCUS       DAYYCY010000141         6150 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000141 DAYYCY010000000
VERSION     DAYYCY010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6150
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.142"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            99..2540
                     /locus_tag="ACHAZB_002255"
     CDS             99..2540
                     /locus_tag="ACHAZB_002255"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0015936 - coenzyme A metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HFM8614887.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(2870..3574)
                     /locus_tag="ACHAZB_002256"
     CDS             complement(2870..3574)
                     /locus_tag="ACHAZB_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8614888.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(3761..4006)
                     /locus_tag="ACHAZB_002257"
     CDS             complement(3761..4006)
                     /locus_tag="ACHAZB_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8614889.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(4023..4874)
                     /locus_tag="ACHAZB_002258"
     CDS             complement(4023..4874)
                     /locus_tag="ACHAZB_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFM8614890.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            5040..6044
                     /locus_tag="ACHAZB_002259"
     CDS             5040..6044
                     /locus_tag="ACHAZB_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HFM8614891.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
BASE COUNT         1974 a         1065 c         1302 g         1809 t
ORIGIN      
        1 aaggattgtt aaaatttaat cttagaaaaa aataactgga acaagagcta tttgttttta
       61 cagcaaagaa tggtatattt ttgtaggagg tggcttactt gaaagaagtc gttatgatag
      121 atgctgcaag aacgccgata ggaaagtatc gcggaagttt aagtccattt actgcagttg
      181 aattaggcac acttgtgact aaaggattat tagataaaac aaaactgaaa aaagataaaa
      241 tcgatcaagt aatatttgga aatgtcctgc aagctggtaa tggtcaaaat gttgccagac
      301 agattgcttt gaacagcggt ctccctgtag atgtcccagc aatgacgatc aatgaagttt
      361 gcggatctgg tatgaaagca gtgatcttag cacgccagct gatccaatta ggtgaagcag
      421 aacttgtgat agccggcgga acagagagta tgtctcaagc acctatgctg aaacagtatc
      481 agtcagaaac aaatgaatat ggtgaaccaa tttccagtat ggtcaacgac ggattgactg
      541 acgcattttc aaatgcacat atgggattaa ccgcagagaa tgttgcaaca caattttctg
      601 tgagcagaga agagcaggat cgctatgcct tgtcgtccca gttgaaagca gcacatgctg
      661 tcgaagccgg tgtattttct gaggagatca tcccagtcaa gatttctgat gaagacgtgt
      721 tatctgagga tgaagcagtt cgtggaaata gtacattgga aaaactgggc acgttacgta
      781 cagtattctc agaagaagga actgtaacag caggaaatgc ttccccgttg aatgacggtg
      841 cctctgtggt gatccttgca tccaaagaat acgcagaaaa taataatctg ccttatttag
      901 caaccatcaa agaagtagcg gaggtcggta ttgacccaag tatcatggga atcgctccaa
      961 tcaaagccat tcaaaaattg acagatagat caggaatgaa tttatcaaca atcgatcttt
     1021 ttgagatcaa tgaagcattt gcagcttctt ctatcgtagt tagccaagaa ctgcagttag
     1081 atgaagaaaa ggtcaatata tatggaggag caatcgcatt aggccatcca attggtgcaa
     1141 gcggtgcacg tatcttgact acattagctt acggattgct tcgtgaacag aagagatacg
     1201 gtattgcttc attatgtatt ggtggaggtt taggcttggc agtcttgtta gaagcgaata
     1261 tggagcaaac acataaggat gtgcaaaaaa aaaagtttta tcagcttacg ccatctgaac
     1321 ggcgcagtca attaatagaa aaaaatgtat tgacacaaga aactgcgttg atttttcagg
     1381 aacaaacact ttctgaagag ctctccgatc acatgatcga aaaccaagtg agtgaagtgg
     1441 aaatcccaat ggggatcgcg cagaattttc aaatcaatgg caagaaaaaa tggattccga
     1501 tggcaacaga ggaaccatct gtcattgcag ctgcaagtaa tggtgcaaaa atctgtggaa
     1561 atatctgcgc agaaacacct cagcggctga tgcgagggca aatcgttcta tcaggaaaat
     1621 cggagtatca agcagtgatc aatgcagtta atcatcgaaa agaagagctg attctttgtg
     1681 caaatgaaag ttatccttca atcgtgaaac gcgggggcgg tgttcaagat atctctactc
     1741 gggaattcat ggggagtttc catgcatact tatctattga ttttttagtg gatgtaaaag
     1801 atgcaatggg cgctaacatg atcaactcga ttcttgagag tgtggcaaac aagttacggg
     1861 agtggttccc tgaagaagag attctattca gtattttaag taattttgct acagaatcat
     1921 tggcttctgc ctgttgtgaa atcccattcg aacgcttagg gagaaacaaa gaaatcggtg
     1981 aacaaattgc caaaaaaatc caacaggcag gggaatatgc gaagcttgac ccttaccgtg
     2041 cagcaacgca caacaaaggg atcatgaatg gtatagaagc tgtagtggca gctacaggaa
     2101 atgatacacg tgcggttagc gcttcgatcc acgcctacgc ggcaagaaat ggattatacc
     2161 aaggattaac agactggcag atcaaagggg ataaactagt tggaaaacta acggtgcctt
     2221 tggctgttgc cacagtaggc ggagcttcca atatcctgcc aaaagcaaaa gcatctttag
     2281 ctatgttaga tattgattca gccaaggaac tagcacaagt aattgctgca gtagggttag
     2341 ctcaaaacct ggctgctttg cgtgcattag ttacagaagg aatccaaaaa ggccacatgg
     2401 gacttcaagc tcgttcatta gcgatctcaa ttggggctat tggtgaggaa atcgagcaag
     2461 ttgcgaaaaa attacgtgaa gcagaaaaga tgaaccagca gacagctata caaatattgg
     2521 aaaaaatccg ggaaaaataa aaaagcaata tctttagaag aataaagcaa gatttacagt
     2581 tattttattt actgcagatc ttgctttttt ctctatcaga aaatatgttt attattagaa
     2641 ttttttatag ttaaggttag gcatcttaag aaatcaggca actccaagat atcttcaggt
     2701 agaagagaag tcgcctaaca ttgtaggatc ataggtgatt tgttggacag gtcaagctga
     2761 tatttgagcc tgatgttaga aggaatttct ttttacttgc tgtagaaatt attatatgac
     2821 taaaaaggct gggattttaa atcccagcct tttatgacaa atagaatttt tatcgatcag
     2881 taatcgctgt cgctgcaaat tgttcgatca aaggaatcaa agtcaatttg actttgtatt
     2941 tccgttcgag tgctttttcg ataacgaact cattttcagt agtcatagaa atagttgcta
     3001 atttacgagg ttcatcagca tttttttcat taataactac ttcatgttca ttttgttctg
     3061 tgtaaaattt aggatactca gcaacggctt ttgaaaaatc agcaaaactt aaagcggcat
     3121 tcgtatcagt tgagcgtgta ttttcagttg tgtccgcttt tggtttttcc gttttttctt
     3181 ctggcacact gactgtttct tctgtaactg gtgtcgcaac agcttgcgca gttggttcat
     3241 cgtttttcaa tcgttgatca ttccagtaag acacgatttc aataagaaga ttgtcacgga
     3301 atcgttcgaa aatcccgttt tctcctgaac gaattctctt taatacagta ttgaagttgt
     3361 catcgattgg cacgttgatc cgtgtatctt catttgtttt cccagctaat ccgatcgtta
     3421 agatttgacg gtttttggga tcaagcatga cttgtaacgg caaaccataa cgtcgcaagc
     3481 tctcttcttc tagagaacgg aataattgtc ctcttacacc atccaatgaa ggacggaatt
     3541 gatttaataa tttttgtttc tgttttttct tcatatgttc tctccttcgc tcataccctt
     3601 tcgattataa gaaaaaaaca gccaaatgaa aaggagtaat gcaaaaaaac ttgcgtatac
     3661 gcaaattaca acagaaaaat ttgcgtattt tcccgttttt tgaaagaaaa ataaaaaatg
     3721 gaacggatta tgtatatata cataatccgt tccattcttt ctattttttc ggtatcaagg
     3781 caaaaacagg tagttcaggt gaataaatga cttcatgttc gtgccagggt ttgaccatta
     3841 gcgaatgatc gaaccgtttt ttcagttctt cgatgatttg atcatgtgtc cagtttcttg
     3901 caggaaaatg ttggattttc tctgcatcga tcaaaaagac aaaatcggaa tgaaaagtct
     3961 gggtaacttc ttccgagaga gaattcaagc gttcgattgg atccataaaa tcacctttcg
     4021 acttaaaatt gggtagtatc tgggtctttt gctttgccga ctacaccatc taactgatcg
     4081 atttgtttca tttcttcttc tgttaattca aaatcaaata attcagtatt ttctttgatt
     4141 ctgcttggtg tcactgactt aggaagagga aggaattcgt gttgcagcga ccaacgcaaa
     4201 gcaacttgtg caatcgtctt gtcatgtgct tcagcgattt gtttcatttc aggcacatcg
     4261 aagattttac cagtgcctaa tggactatat gcttcgagga tcatatcatg tttttttgca
     4321 tactcaacta cagttgtctg taattcgcct ggtgcaagga aaacttgatt gaccatcggc
     4381 ataatttttg ctgtttttgc tagtgtatct agatggtggg gtaagaaatt gctcacaccg
     4441 attgctttga ttttacctgc ttcatacagt tcttcaaatg ctcgccatgt atctgcattt
     4501 gcttgttccc aattatcacg aaaagcaaca gggtttggcc agtggatcaa aaacagatct
     4561 aaataatctg tttggagctt cttcaatgat tcttcaaaag aagacatcac aagttcatag
     4621 ctgtggtttg cattccataa ttttgttgtc aagaatatct cttctctagg aattccactt
     4681 tccttaatcg cttggccgac gctttcttca ttcttgtatc cttgggcagt atcgatatgg
     4741 cgataacctg cagccagcgc ttctttaaca gaggaaacag cgacatcccc atcaggtgtt
     4801 tgccatgtgc cgaatcctac taccgggatt tcgtaaccat ttgatagacg ataagtgctt
     4861 gttaatgaat tcatactttc agctcctttt ttattcgttc aactataagt gtagcgcatt
     4921 tttcgaaaaa aagaatccat tttgtttcta ctcttgaaat ttttgtttaa aagaaaatga
     4981 ttttgattct ggtatttttg attttttttc cgtaaactaa gacagaaggg aatgagaaaa
     5041 tggatatcta tttaaaaaaa gcaattctgc atattattga cagagaaact ggttctccag
     5101 tcttttcaca aaaagagctg gatctgacaa aagaatatat tcgtgatttt cttcaaaaga
     5161 agatccaaaa aatctcttct gctcaaacaa aaactggaca actagcagaa gaaagcatga
     5221 tcgttgaatc acttaaaatc gctcaagaaa attttgtgga agcaagtgaa gcaatcgttc
     5281 agcggtggtt cgatatctac caagaaagtg aagaagcacc tagtgcagat gtatttgttg
     5341 tcttgtatga attagataca gtgatgtact tatctttatt gaaagtcaat tatcgtgagg
     5401 cttacactca ctttgttgaa gcagacgaag cagggatcga caaccgcttg attttgaatc
     5461 gagcaattct aggggcgaaa acacaaaaag cagatgaagc attcgctgtg aatttgactg
     5521 attggaccta cgaattaatt gaaaaaaaat atgagttttc tggcaaaaaa gagctttatt
     5581 tttctggaag agtgatcgaa agtgttcctg caccttcttt agaagaaaat gtaaaagtga
     5641 tccaaaaagt agccaagcat cttggaaaaa aatttgagac agaagaattt gatatcatgg
     5701 ctgatttgaa agaagctgtc tatgatacga tagaagaaaa aggtcggttg gatcatgaga
     5761 tgattgcgga aaaagtcttc aaggaaaaca tcacggcaaa aatggctttt aaagaagaag
     5821 tacaagagca aggatttgtc ccagaagcac ctcctgtaaa agaagtacaa gaaatttctg
     5881 agaaaaaatt tggaaaacaa aaattcaagc tatcaaacgg gatcgaactg atcgtaccag
     5941 tagatgtata tcgcaaccca gatttgattg aatttgtgaa taatccggat ggtacgatat
     6001 ccgtgatgat caaaaatgtg gatgaagtat tgaatcgttt ataaaaatga ctagaatatt
     6061 aggtgttcaa gtaccagtga tcagttaaat gaattgtgtc aagagaagga tcggaaaaga
     6121 cgaatataac aaaaaaagag cgtgagacaa
//
LOCUS       DAYYCY010000142         6116 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000142 DAYYCY010000000
VERSION     DAYYCY010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6116
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.143"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(20..208)
                     /gene="rpmB"
                     /locus_tag="ACHAZB_002260"
     CDS             complement(20..208)
                     /gene="rpmB"
                     /locus_tag="ACHAZB_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFM8614892.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            478..840
                     /locus_tag="ACHAZB_002261"
     CDS             478..840
                     /locus_tag="ACHAZB_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFM8614893.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            892..2574
                     /locus_tag="ACHAZB_002262"
     CDS             892..2574
                     /locus_tag="ACHAZB_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HFM8614894.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            2691..4727
                     /gene="recG"
                     /locus_tag="ACHAZB_002263"
     CDS             2691..4727
                     /gene="recG"
                     /locus_tag="ACHAZB_002263"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFM8614895.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            4776..5777
                     /gene="plsX"
                     /locus_tag="ACHAZB_002264"
     CDS             4776..5777
                     /gene="plsX"
                     /locus_tag="ACHAZB_002264"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFM8614896.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            5899..>6116
                     /gene="acpP"
                     /locus_tag="ACHAZB_002265"
     CDS             5899..>6116
                     /gene="acpP"
                     /locus_tag="ACHAZB_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFM8614897.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAV"
BASE COUNT         2027 a         1065 c         1411 g         1613 t
ORIGIN      
        1 cggtccaagt atcattcaat taaacgcgtt caacttttcc tgatttcaaa gcacgagttg
       61 acacccaaac ttttttaggt tttccgtcga ccataacgcg aactttttgt aagttaggtt
      121 taacagtacg ttttgttgag ttcatagcgt gtgaacggtt gtttccactt tttgtcttgc
      181 gacctgtaaa gtaacaaact tttgccatgg taatttcctc ctttgccttg atttggagca
      241 atagaataat tcagctcata ccagactaac atatcatata gaatgggaat ttgcaagtac
      301 attttcatag atacaattga caattgagaa gaataattgt aaattaaaca aggttatacc
      361 tgaaattttg caaggttttt gagtactttg ggtaactgcc ttttatttca agttgcgcta
      421 tgataaaata tgatagaaga aaaatgggag ttttttaccc gttaaggagg ctcattcatg
      481 gctgtaaaaa ttcaaacgtc agctggaacc attgaaatta ccaatgatgt aattgcaaca
      541 gttgttggtg gcgccgcgac agatgtgttc ggtgttgtag gaatggccag caaaaatcaa
      601 attaaagata atattactga gattttgcgt aaagaaaatt actcaagagg agtagttgtc
      661 cgccaagaag ataatggtat tgctgtagat gtttatacga ttatcagcta tggtacgaaa
      721 atctctgaag tttcccgcaa cgtccaagaa aaagtgaaat acaatctaga ggcattatta
      781 ggcgtaactg ccaattcagt taatgtcttt gtacaaggtg ttcgtgtact gccagactag
      841 gcaggagact ggctggtaac tcagcagtcg atctgaagga ggattatcta ggtgagtgta
      901 acagaaataa gtgcaagcca gttccaggaa atggtccagg caggtgcgaa tcgtctgcaa
      961 gtaaatgcag aatacgtcaa ttcattaaat gtattccctg tgccagatgg cgatacggga
     1021 acaaatatga atctatctat gactagtgga gctaaggcag tagtagattc aacctcagaa
     1081 aaagtaggag aattagcagc aatgctttca aaaggattgc tgatgggtgc gcgcggaaat
     1141 tccggcgtta ttttgtctca attgtttcgt ggattttcta agcaaattcc agatgttgtc
     1201 tcattaaatg cttctgatct tgctgcagcc ttcactcatg gagttgaaac tgcgtataaa
     1261 gcagtaatga aaccagtaga aggaacgatt ttgacagtag ctagagaagc agcgaaagct
     1321 ggagaaaaga aagcaaaaag tactgatgat gtcgttgaag taatgacagc tgttgtaaaa
     1381 ggagcaaagc gtgctttagc taaaactcca gacttgcttc cagtcctaaa agaagtcggt
     1441 gtcgtagaca gtggagggca aggactgctg tttgtttatg agggcttttt aagtgcattg
     1501 aatggagaat tccaagcgga tgaaacatat gaaccttctc cagcagaaat ggatgatatg
     1561 gtcaatgcag aacatcatcg cagtgtccaa ggtcaaatgg cgacagaaga tatccatttt
     1621 ggttactgta cagaaatcat ggtaaaaatc ggagaaggac ctacagtcga tagtacattc
     1681 gattacgaag aattccgtaa ctatttagat ggtataggag attctttact tgtggtcaat
     1741 gatgacgaga tcatcaaagt ccacgtgcat acagaaaacc caggagaagt cttgaattat
     1801 ggacaaaaat tcggttcatt agtcaaaatc aaagtcgaca acatgcgctt gcagcatgag
     1861 acgattttgg aacacgatga agaagtgaac gagtttgcga aaaaagaaat accacgtatc
     1921 ccgtatggta tcgtagcgat cgctgctggt aaaggcgtcc aagagctgtt tgaaagttta
     1981 ggcgctaatt atgttatcag tggtggacaa acaatgaatc caagtacgga agacattgtt
     2041 aaagcgattc aagaagtaaa cgcagataaa gtgattgtct tgccaaacaa taagaacatc
     2101 ttcatggcag ctgatcaagc agcagaagta gcagatatcc cagtagcagt tgttccttca
     2161 agaacgattt cccaaggatt gacagctatg ctttcattca atgatcaagc ttctttagaa
     2221 gaaaacaaag aaacgatgac agaaatgttg tctagcgttg tgagcggtca agtcactcac
     2281 gcgatcagag atacctcaat tgacggtgta gagatcaaag aaggagactt tttgggcatg
     2341 atcgatggaa aaatcctgat ctctaaccca gatatcacag caacagcgtt aagtacacta
     2401 gaacaaatga tcaatgagga cacagagatc gtaacgatct tggcaggtga tgaaggaaca
     2461 gttgaacaag caaatgaatt tgctgagaaa ctaacagaaa aacatcctga tttggaaatc
     2521 gagatccacc aaggagatca gcctgtctat ccttatctat tttctgctga ataatttctg
     2581 tgtcagaata tctttctaac acagtattca ttcataaaag gccttcggtt agtgatcagg
     2641 tatgatcgct gaccaagtcc tttttttgtt aaaggaggga ggaaaaattt atgcgttctt
     2701 tagatgatcc aatcactatg ctggcaggag tcggagaaaa aagagcagct agtcttgcca
     2761 gcttgggcat ccaaacgatt gaagacttac tgacttatta tccttttcgc tacgaagata
     2821 ttcaggaaag aaatctaaac gagatccagg atcaagaaaa ggttactttg aaaggaatcg
     2881 tagtctcgcc tcctgtgatg aatcgatttg gttataaaaa aagccgattg caatttcgta
     2941 tgatgcaaga acatgatgtg ttcaacgttt ctttttttaa tcaaccttat ctaaaagaaa
     3001 aagtcatttt atcggaggaa atcgccgtat acggtaaatg ggatgcaaag cgtaaagcct
     3061 tgaacgggat gaagatttta ggttctcaaa caatggatga cttttcgccg atatatcacg
     3121 tcaacaaatc aattcgtcaa acgacattag ttgatctaat caaaaaaggg tttcaagaat
     3181 acggcacatt gattgaagaa aatctgccaa acaaccttgt ggagaaatat cgactgcttg
     3241 accgagcttc tgctgtaaga agtatgcatt ttccaaaaga ccacgaagaa aaccatcaag
     3301 ccaaacgacg tgtcgttttt gaagagttct ttttatttca aatgcgtctt caaggattga
     3361 aaaaagcaga aaaagcagaa acgaatggtt tggagatcct ttatgatgtc caaaaattga
     3421 aacaatttac tcaaaagcta ccttttgaac tgacaaatgc acaaaaacgt gtaacgaatg
     3481 aaatctgtcg ggatcttcgc agtccgcagc atatgcagcg acttttgcag ggagatgtag
     3541 gtagcggaaa aacagtggtt gccgcgattg cgttatacgc taccgttaca gcaggtttcc
     3601 aaggtgcgtt gatggtgcct acagaaatct tggcacaaca acatatggaa agcttgcaac
     3661 agctgtttga tcccaatgaa gtaaccatcg cattgattac ggggtcaacg aaagcaaaag
     3721 aacgacgtga gctccttgaa aaactagagc aaggggaaat taacgtcgtg atcggcacac
     3781 atgcattgat ccaagatgga gtcgaatttc agaatttagg tctagttatt acggatgagc
     3841 agcaccgttt tggcgtgaac cagcggaaag tacttcgtga aaaaggctgg cgaccagatg
     3901 tcttatttat gacagcgact ccgattccta gaacattagc tattacagca tatggtgaga
     3961 tggatgtatc tatcattgat gaacttccag ctggacgtat cccgatcgaa acacgctggg
     4021 taagaacacc tcaattggac agtgtactgg attggacgaa aaaagaactg gcaagaggac
     4081 atcaaatgta tgtcatctgt ccgctgattg aagaatcaga agcattagat gtaaaaaatg
     4141 cagtggaaat ctatgaaaaa cttcgtgaac tatttgcacc aaaatatgaa gttggcttgt
     4201 tacatggtaa aatgaagaat caggaaaaag aagtaatcat ggaagccttc aaagacaacc
     4261 agatgcagat attagtttcg actactgtaa ttgaagtagg ggtaaacgtg ccgaacgcga
     4321 ccgtcatgct gatcatggat gcggatcgat ttggattggc acagctgcac caacttcgtg
     4381 gacgtgttgg acgcggaagt gatgcttcct actgtatctt agttgctaat cctaaaaatg
     4441 aactaggtgt ggaacggatg aaaatcatga cagagaccac caatggtttt gtactaagcg
     4501 aaaaagatct ggaattacgt ggtcctggtg aagtatttgg cttccgtcaa tctggtctgc
     4561 ctcaatttgc gattgctgac ttggtgacgg atggaaatgt tttggaggta gccagagaag
     4621 aggctgcagc cctttggcaa ataaaggatt ggcagatgct tcctgagtat cagcctttag
     4681 cgcaaagcct ccaaatagaa aatcaggata atcgttattt cgattagcag actgtgctat
     4741 actttatact gatgacggaa aaaggagtgg aaacaatgaa aattgccgta gatgcaatgg
     4801 gcggagatca cgcacctcaa gcaattgtag aaggcgtggc tttagcgcag caagattttc
     4861 cagaagtaga attcttactt tacggaaaag aagcagaaat caagaaatac ttaacaaatg
     4921 aaaaaaacat cacgattatc catacagacg aaaaaatcaa tagtgatgat gagccagtaa
     4981 aagcaattcg acgaaaaaag actgcatcaa tggtccttgc tgcacaagca gtgaaaaacg
     5041 gtgaagcaga tgctgtattt tcagcaggga atacaggcgc gctactagca gcagggttgt
     5101 tcatcgtagg acgtatcaaa aatatcgaac gccctggctt gatgtctacc ttgccagtag
     5161 tagggaaaga aggtgccggt ttcgatatgc tggatcttgg cgctaacgca gaaaacaaac
     5221 cagaacattt gttgcaatac ggtattttag gttcttttta tgcacgtaaa gtccgaggta
     5281 tcggacgtcc tcgtgtcgca ctcttaaaca acggaacaga agcaacaaaa ggtagtgaag
     5341 tgacgaaaaa agcctatgaa ctccttcaga atgaaacaag cttgaatttt attggaaacg
     5401 tagaagctcg tgagctactc aatggtgtgg ctgatgtagt ggtcacagat ggatttacag
     5461 gcaacgctgt tttgaaatct attgaaggaa cagcaatgaa tatgatgaat ctattaaaat
     5521 ctgctattct aaatgaaggg atcaaaggaa aaatgggcgc tatgctttta aaagacggct
     5581 tacgttcttt gaaagcagaa atggattatt ccaaacatgg cggtgccgtt ttgtttggct
     5641 tgaaagcacc agtcattaaa actcatggag caacaggccc cgatgctgtt cgctatacga
     5701 tccgccaaat ccacacaatg cttgagacag atgtagttgg acaattagtc gaacaatttg
     5761 aaaaaagcga agaatagtat ttggatatct gaagcgatcc gttccattga aaaaaaggtg
     5821 gacattcctg aaaaatgcca gtaaaattag ccatggtgta ggtaattgac aagtgtatga
     5881 ttcttggagg tgaaaaagtt gactcgcgaa gaagtattta acaaagtagc gaaaattatt
     5941 tcaaatcatt ttgagataga taccgataaa gtgacggatg aattgaacat caaagatgat
     6001 ttaaaagctg attcaattag tattatggaa ttcgttttag agcttgaaga tgaattcggt
     6061 acggaaattt cagatgaaga cgctgaacaa attgaaacag tcggtggcgc agtaga
//
LOCUS       DAYYCY010000143         6058 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000143 DAYYCY010000000
VERSION     DAYYCY010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6058
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.144"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..464)
                     /locus_tag="ACHAZB_002266"
     CDS             complement(<1..464)
                     /locus_tag="ACHAZB_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8614898.1"
                     /translation="MKGFMNMFTNDQFKSLLIKTLERKSSSNYYEGGNEIVSKMKISE
                     VTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGD
                     NVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRV"
     gene            complement(506..727)
                     /locus_tag="ACHAZB_002267"
     CDS             complement(506..727)
                     /locus_tag="ACHAZB_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8614899.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            complement(724..1014)
                     /locus_tag="ACHAZB_002268"
     CDS             complement(724..1014)
                     /locus_tag="ACHAZB_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8614900.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            complement(1011..2195)
                     /gene="mobT"
                     /locus_tag="ACHAZB_002269"
     CDS             complement(1011..2195)
                     /gene="mobT"
                     /locus_tag="ACHAZB_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFM8614901.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            complement(2377..3780)
                     /locus_tag="ACHAZB_002270"
     CDS             complement(2377..3780)
                     /locus_tag="ACHAZB_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFM8614902.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            complement(3802..4575)
                     /locus_tag="ACHAZB_002271"
     CDS             complement(3802..4575)
                     /locus_tag="ACHAZB_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8614903.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            complement(4585..4962)
                     /locus_tag="ACHAZB_002272"
     CDS             complement(4585..4962)
                     /locus_tag="ACHAZB_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFM8614904.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            complement(4983..5297)
                     /locus_tag="ACHAZB_002273"
     CDS             complement(4983..5297)
                     /locus_tag="ACHAZB_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFM8614905.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            complement(5497..>6058)
                     /locus_tag="ACHAZB_002274"
     CDS             complement(5497..>6058)
                     /locus_tag="ACHAZB_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFM8614906.1"
                     /translation="LKNEFGIEVKDYFIDLDLLQLINSVKRMYSGDDNGVDLYKFVTK
                     HIFVNLNIDEKNYPSIFKHYLFFINEVEDRIKNNSLSSSVSSFKKMFKSRDGITLTTA
                     HKVKGNEYDTVIAINLLNGKIPHWNEIFNVSDKGISSARKLLYVICSRAKNNLFLFSE
                     VGYFTSKGDLLYPTQLLDKIEFDYNI"
BASE COUNT         1815 a         1186 c          995 g         2062 t
ORIGIN      
        1 tgaactctgt caaaggcacg ttgatattca ccattactca taaaagactc agcgtcttca
       61 atagcttgtg aaaccacatc actaattttc aactgactaa agttaattac ttcgtaattc
      121 tctggaagtg gtactaaaat agtatccact aatacattat caccttgttt atcatatatg
      181 gagcttgctg tctgggtaat caaatcttta tgactttcaa aaaagtctaa atcatcaaaa
      241 ggaactctta ttttaagata tgtgcaaagc gtattccagc cctttttata tcctttataa
      301 taagtaaaat cgtcagtctc atctaaagtt acctctgata ttttcatttt agaaacaatc
      361 tcgttacctc cttcataata atttgaagag ctctttcttt ctaatgtttt aattaaaaga
      421 gatttaaatt gatcatttgt aaacatattc ataaatcctt tcataacaaa atatatactt
      481 cctattttat catgaaaaat atctgttaag caccaatgat tcggttaaat aagtttaata
      541 acacatcttt cacgccagct gcattgaaca caagtccgac ggcgattaga gcaataatca
      601 agaagccaat gagttttgaa aattctcgtt taaaacctaa aaacagtcca atgacaacaa
      661 tcgctaacaa taccaatgac tgtgcattat ccaagaacca gttgtacaaa ttctgcccaa
      721 aattcatgtg attgtcctcc tttccttaag atatttttct cgataataga tatagctttt
      781 tacacgcctt aataattgaa aaggggttaa atgctctcca tagacggtta ctcgattggt
      841 ataattatta taaaatctca ccttgtaaaa atcataggta tagatggtat caccagcttg
      901 ccgttttccc tcaataatga gagacgtata tttccctgta ttttgtatat acgcttgaaa
      961 ctcaatgcca cactgaaacc ctaacttctt taattctttt gttaaaaggc tcatgtaatc
     1021 aaatcctcca tggctagatg ttgttgttca attaatttct catgtttttc tgtcagtttg
     1081 gcttcctgaa tcatggtgga gataatggtt gtaccgttga gaacatctaa aatgcttgcg
     1141 atttttaatg taggggcgac ttgatgatgt aaccagttca atgttcgttc aaaagaataa
     1201 ggctctggct gtgtggttag gcgtaatgct ccacgatttt taccgataaa ccattcccaa
     1261 cgttcattag ttttccaatc actccgacgt ttagtgtcgt ctttatctgc aaagcggata
     1321 tagcgattga taatttcaaa ggctgttttt tccgcctgtt gatgtgccaa taaatctttc
     1381 atggcttggg tggctcggtc attttttaac cgaatttcaa accgattctt tacttctgcg
     1441 tcttctaagg gaacatcatt tttcacgtat tgctcataat ctttctcata tatacagaaa
     1501 tagacttcac tttttaatga gccaatataa agtgtgtttc ccatgtatgg tttttcatca
     1561 cggtgaacca attcaccact gcgataattt ttaaagcttc gaaagacgga aatgcattct
     1621 tcctgtttac atttctttgc caaatctgga atattcaaaa tccctgtttt atcgttgata
     1681 gctaaatcca agcgtttgaa aataccttta tgttccatgc aatcttgaaa gaaatcatac
     1741 cagctccgat gttgagccaa tagaaagttt tcaaattgcc gacagccttt tcctttcagt
     1801 tctaataaaa cacctttact tacatcatga gagactagga caacaatatc accgaaacga
     1861 tagtgttcgg gataagaata atagccaaag tcttcgtgta gcatataatc catattcaaa
     1921 cgtaaaacct caccgataat ttgagccaca tcattggtag ggaatcgaat ccgtacatag
     1981 tcgaataaca tctccaatgg gttatcggga ttgaagcgtt ccaaagcgtt cagtaaatct
     2041 tctttcacct gttgagtagg gactgccttg cctgtttcaa tatccgatag gtaaggtctg
     2101 gtaatatgag aagcaaccgc cagtttattt tgagaaacgc cataagccag tcgcttttct
     2161 ttcaattcct taatccaaat tttttcgttt tccattcgca aacctccaat ctaaaaaaga
     2221 tgtaaactat gacccttgag tttacacctt tcatattttc cgcaatcctt tgtaccatca
     2281 acattggttg atgagaattg cctgttttca gttgtatttg tgcccccctg ttagatagag
     2341 ggggctaaag gattggcgtg gctaacgcca caccaatcaa agcaagtctg aacctgtacc
     2401 tttcacttcg tgcggtacag gctcagattg ctttttttga atgacttttc ctatttctcc
     2461 taagaaatcg tacctttttg gtacaagggg tgtataaaac tcactaatca cactttttcc
     2521 tgtatccacg taacctcttc ccttgatggg ttttaagaaa aattgttttt gtgtatcgct
     2581 tccgaacatc ataccaaagc ccatttcaga catacgaccc aaagccacac ggaagttaaa
     2641 gttgtcccga atcccatcac taaaatattt tgcgtctgga cgttgacagg caagaattaa
     2701 aaagaagcct gcttgtcgtc ctaacattac aatttgtttg agtttatgaa taacggtggt
     2761 actttcacga cctagcatat ccatcattgc cacatattca tcaaaaataa gaaaattggc
     2821 agacaaacca agataagcat aattttctcc tgttttatag ttagggtgtt gtttcatggt
     2881 ttcactgcga tcaatcatat cttcgtaaaa ttgattgata catgccaaca tatcttcttt
     2941 acggtaatag acattattca tgaccgcccc taagtctgcc aaatcagcat ttttagggtc
     3001 aagaatataa agctttgcat ttgtatgcag gagagattca atcaatgtga gaataaaata
     3061 ggattttcca ctaccagtgc ccccagcaat taacatgtga gggagagaat cgaactccca
     3121 atagaccgat tccatcaatt tgacacgtcc atcttgagct accacgtctt caatgccaat
     3181 tcgattgcca atagtatcat aaagtaggac atactctaca taagaatctt tcaacgtttt
     3241 ttccactaat tcacaataca atcctgtttc tagtttgttt tctaggtgca aaagctgatc
     3301 ttgatatttc cctaaggtga tttctacatg aatatggatt agtccatctt tcattcgata
     3361 atatatttta ggaaaatggc ggattcgttg tttggttttc ttgctgggta aatctttaaa
     3421 gaatccctct gattgagttt cttcggcttc ataccaatga ttttctaaaa tcatacgagc
     3481 taacttttga cggtgctcta actgttttat agaatctata cgatagtgat aataaagcca
     3541 gctgataagt ccgcatacaa aaagagcagt gagaatactg ctccaaaagt atggcgtatg
     3601 aagttgatag aaaaatgtat ctaactgaac tgcttgccag ttcaccgatt gtaacgctct
     3661 ccaatgaaaa gggagtagga cgactagaaa aactaccagc aatgaaatta gtacaaaatg
     3721 atagaccaag tgcttatcgt tggctttgat tcgctctcct ctgtcatgaa gcaatggcat
     3781 aaaaatcctc ctttccacct atcaaaataa ttgtgtattg tagatacgag cctgtttatc
     3841 aaaagttggt tgagaaatgg cttcatattt ctgataagcg attcgctttt caaagcgtaa
     3901 atttttttct ttcggataat aatgacgtcg atagagagtg tgtcctaaca gttttttcgc
     3961 ttcttttgtc aacggataga gatagcgaaa catgcgtcca ttgattttct caatcccttt
     4021 gtattcgcaa aatgcctggg aaagccaatg ctttttctct acctgttcaa agcgagcatt
     4081 ttcctctaat aaaaggcgag cacttcgagg gtgtatcttt tcccatgact gcttatcacg
     4141 atagacactt gtcttgaaat acccgcagta aaagaagttg gacgcttgat agacatagcc
     4201 acattttccc tcaattccat ctgccagtgt atataaaaaa tgacagtccg tatgttcttt
     4261 caaccactta attagagctg aaagagcttg actgccaaaa taattggttt gattcatttc
     4321 tggaagaaaa cacatctttc caatttctaa ataatctgtg gtttgaaggg aactgtcggg
     4381 aaagagtttg cgtatggttt gtaggggttg cgtaccccag cctaattcca caacgcctaa
     4441 taatttttct tctgaaaaaa tccctagaaa gtatttacac aatctaggga gtatctttga
     4501 ataatggtat tgccgaataa aagaaatggc ttcatccttc ggaatttctt tcaaaatcaa
     4561 tggtgtgttt gtcataagca acacctattt ttcactttcg gacgcttctt ttttatattt
     4621 ctgatgaggt tttgtctgtc cttttaatac aagatcgtct gctttcacgt accaatcaac
     4681 gtctgcccct cgaaaagtag ccgtcgctac ggtatctgcc acaggattga ttaattccac
     4741 ttctgcgttg tagtcaaatt ctttcaaagg aacagaagcg gggatactga cttgtatcat
     4801 gcgtccttgt ccttttgatt ttaagtcata ggtacgttct ttaatttctt cggaaaccgt
     4861 accatcttca ttctggatac gaacctcacg acgcaaggcg gaaaatttca atgtgccaaa
     4921 tgtctggtct ttttcaatca caataccttc tgctaatctc atattttttc acctttcctt
     4981 tcttatgctt tcaccatatc gtctgcgtgc ataatgtaat tggtaaatcc tcgattgcca
     5041 attttataac cttctgcggt aatatgtggg ttgatgagtt tgactttttc gtcggattca
     5101 aaatgtttct cgccagcttc ggagggaaga atgacaacaa tatcatctgc tcgttgaatc
     5161 gttgagtata aattgtaact acgagagaga atggttggtc tgccatttat tcgacgttgt
     5221 tcaatgttcc cttcaccagc atattctaat tgtccaaatg ttttttccat attgggaatg
     5281 acatatttaa gttccatatt tttacctatc ctttctgttt ttatgtttga atgaatctta
     5341 tttgtgtgat ggttttaaag gggggagagc gaccttttga ttcttgattt tttattttca
     5401 taagttcact tcctttcaaa attgggtaaa aaaatagacg ctccgtactg agcgtccgat
     5461 tcctactata tttaaaacaa cgactaccta ggtattttaa atattataat caaattctat
     5521 tttatctaat aattgagtgg gataaagcaa gtctccttta ctcgtaaaat aacccacctc
     5581 tgagaaaaga aataaattat tttttgctct tgaacaaatt acgtatagta gttttctcgc
     5641 tgaactaatt cctttatcag aaacattaaa aatttcattc caatgaggaa ttttcccgtt
     5701 tagaagatta atagcgataa cagtatcata ctcatttcct ttaactttat gtgctgtagt
     5761 aagtgtgata ccatctcgag atttaaacat cttcttaaaa ctagatacac tactagataa
     5821 tgaattattt tttatccgat cttctacttc gttaataaaa aatagatagt gcttaaaaat
     5881 agacgggtaa tttttctcgt caatattaag attaacaaat atatgtttgg taacaaattt
     5941 gtataaatca acaccgttgt cgtctccact atacattctt ttcacactgt ttattagctg
     6001 taataaatct aagtcaataa aataatcttt aacttctatt ccaaattcat tttttaat
//
LOCUS       DAYYCY010000144         6002 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000144 DAYYCY010000000
VERSION     DAYYCY010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.145"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..395
                     /locus_tag="ACHAZB_002275"
     CDS             <1..395
                     /locus_tag="ACHAZB_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFM8614907.1"
                     /translation="ISLKDESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQE
                     YYFYKCITKRVCCICGKTGADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKI
                     GVINFKNKYQIKGIKLSHETIKKLRIGG"
     gene            398..1228
                     /locus_tag="ACHAZB_002276"
     CDS             398..1228
                     /locus_tag="ACHAZB_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HFM8614908.1"
                     /translation="MAEISWIKLKTTMFDDEKIRLIQAVPESDAIIVIWIRLLVLAGK
                     TNDDGLIYIQRNMPYTEEMLATLFGKNVNTVRLALTTLANFNMIDLSSDGLIAISNWE
                     KHQNIEGMDKVRLKNAERNRKYRERKRQERLKLENDVSVTSRDGTDKDIEEDKDIDKE
                     EKKGKYSDEHLRLAKKMQRNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEYV
                     INWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKKNHKKQSQKLQYSNPSEYDDLPI
                     "
     gene            1244..2095
                     /locus_tag="ACHAZB_002277"
     CDS             1244..2095
                     /locus_tag="ACHAZB_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8614909.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALERAKSYVNDVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNAELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            2092..2451
                     /locus_tag="ACHAZB_002278"
     CDS             2092..2451
                     /locus_tag="ACHAZB_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HFM8614910.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCSQYRV
                     PLILAKKYRNTFKEERF"
     gene            2466..2627
                     /locus_tag="ACHAZB_002279"
                     /pseudo
     CDS             2466..2627
                     /locus_tag="ACHAZB_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
     gene            2624..2929
                     /locus_tag="ACHAZB_002280"
     CDS             2624..2929
                     /locus_tag="ACHAZB_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8614911.1"
                     /translation="MSVDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWAEKPK
                     RKTPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            2929..3240
                     /locus_tag="ACHAZB_002281"
     CDS             2929..3240
                     /locus_tag="ACHAZB_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFM8614912.1"
                     /translation="MNELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGANIQECLEMAYNEIKGRTGKMVDGVFVKSSDLEDS
                     E"
     gene            3237..3410
                     /locus_tag="ACHAZB_002282"
     CDS             3237..3410
                     /locus_tag="ACHAZB_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8614913.1"
                     /translation="MMMPKEIYSDALRGARNQLKMAKRAYEIRPTIENERRVKAIRRR
                     CSIYGELQKEDSK"
     gene            3407..3823
                     /locus_tag="ACHAZB_002283"
     CDS             3407..3823
                     /locus_tag="ACHAZB_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HFM8614914.1"
                     /translation="MIPRFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIELMQSTGLKDKNGIEIFEWDIVSVSVRNGFDYLDNKVCVVKNSIGHSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVIGNIWENSELLEEQ"
     gene            3823..4491
                     /locus_tag="ACHAZB_002284"
     CDS             3823..4491
                     /locus_tag="ACHAZB_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HFM8614915.1"
                     /translation="MNKQELIDELAKYVKRYENVMDEHGQGRYGAYEVSLKLVKRLNE
                     SKITDEQAWNKVAEAYPESAQSLRNTLDNAVFGKTGEPQKHVMPKFVAEWIEYAKKKG
                     DSLAISFKPWNLYGVEYSKADRWIGDNQETFARAWLDGYEVEKEPLYYVPLPYEVWDE
                     EAAELKTEYLYLHYEITSDETRIFPTKEPRKGFVAKLDELTIKSADENYWPFAVPVEE
                     VVEG"
     gene            4488..4865
                     /locus_tag="ACHAZB_002285"
     CDS             4488..4865
                     /locus_tag="ACHAZB_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8614916.1"
                     /translation="MKKLHQLISEKESELQNLEDSLGLGFPIVEQVKMVQISHLQLEL
                     EDLRQIEDPYQLNDNQQIVLEWLKLTASTGKPMQVVFWMMNNAAWGHLDELRDPLMEL
                     TDKQQFEVLTAFAQWGLEQEEKE"
     gene            4862..5161
                     /locus_tag="ACHAZB_002286"
     CDS             4862..5161
                     /locus_tag="ACHAZB_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8614917.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKLLAKSKDNLAVVRLVDMEVINNEI"
     gene            5151..5309
                     /locus_tag="ACHAZB_002287"
     CDS             5151..5309
                     /locus_tag="ACHAZB_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8614918.1"
                     /translation="MKFKIFEEDTRYKLEKKLNEFAKNNEIQHISLTASKSGYTTYYA
                     AVVSYVIQ"
     gene            5403..5819
                     /locus_tag="ACHAZB_002288"
     CDS             5403..5819
                     /locus_tag="ACHAZB_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFM8614919.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
BASE COUNT         2231 a          899 c         1391 g         1481 t
ORIGIN      
        1 aaataagcct caaagatgaa tcgaatacga ctgtgagtga tgctaatgtc ttagcaaata
       61 tcatcttaga tttcatcttt gaacatcata ttcctttcaa agaaggctat gagattttac
      121 cagcgaatca agaatattac ttctacaaat gcattacaaa aagagtttgt tgcatctgtg
      181 gcaaaacagg agctgacatc gatcactttg acaaagcgct aggaagacga aagcgaaaag
      241 aagttgatca ttcagagtac acatttgcag cactctgcag aatccatcac acggagaagc
      301 acaaaatagg tgtgatcaat tttaaaaata aatatcaaat caaaggaatc aagttaagtc
      361 atgaaacgat taaaaagtta aggataggag gataaatttg gctgagataa gttggatcaa
      421 acttaaaact actatgtttg acgatgaaaa aatacggtta atccaagctg ttcctgagtc
      481 ggatgccatc atcgttatat ggattcgatt actagtttta gcaggaaaga ctaacgacga
      541 tggtctgata tatatccaga ggaacatgcc ttataccgaa gaaatgcttg ctacattgtt
      601 tggcaaaaac gtaaatacgg ttcgcttagc gctaactaca ttggcaaatt tcaacatgat
      661 tgatctaagc agtgatggac taattgccat cagtaattgg gaaaaacatc aaaatatcga
      721 gggtatggat aaagtaaggc taaaaaatgc tgaaagaaac cgtaaataca gggaaagaaa
      781 gagacaggaa cgtctcaaat tggaaaatga cgttagcgtg acgtcacgtg acggtacaga
      841 taaagatata gaagaagata aagatataga taaagaagaa aagaaaggta agtattctga
      901 cgaacactta cgccttgcta aaaaaatgca aaggaattta actgaagatt ttccaaaaga
      961 aatgaacaaa gtagatatcg aaaaatgggc agacacaatc aggttgatgg aagaaagaga
     1021 taaagcatct atagaagcga ttgagtatgt gatcaattgg ctacctacaa atgaattttg
     1081 gtttggaaat attagaagtg ctaagaaatt gagagaaaaa tttgagaagc tcaaattcga
     1141 aatcaaagca gacaagaaga atcataaaaa gcaaagtcaa aaactacagt acagcaatcc
     1201 tagtgaatat gacgacttgc caatttaaaa ggagatgcat cacatggaaa gcctagcaaa
     1261 tgctatggag aagctaataa gaagagtatt agtgcaaagc ggaaaatgtc cagaatgtag
     1321 cgaacctttg tatagttggc gagctaaaaa taaggatggt tcagaacgtt gtaaaccaac
     1381 atgtatgagt tgtggttata aagcgttacg tgtgaaagag gatatacaga ccgaacggat
     1441 atataacgac agcttaaaag cacgagcatt gagttttttt caaaatggtt cggtattaac
     1501 agataaaact ttgtttaaat gcaaaatgga gaattatcac gtagtggacc aagaaacgaa
     1561 aattgcttta gaaagagcta aaagctatgt aaatgatgtc ctactgaacc atcctgcaca
     1621 tttcattcta tcagggaaat caggaagcgg aaaaagccac ttatcaatgg cgacagcttg
     1681 ggaaatactt gagcgctcaa attatgacaa gaaaatactt tttataagct atcaagagtt
     1741 attagagcaa ataaagtttt cttataacaa tgctgaactg agaaaagaaa ttgaaggatc
     1801 gcttatagcc gatatcaaaa caactgattt ggtggttttt gacgatattg gagctgaatt
     1861 aggtagcggg gtatcaaata gtaggcaatt tacaaacaac acgttaaaca cgctcttaga
     1921 agccagacag aacaaggcaa cgatcatcac aacaaactta tctggtcctg aactaagaga
     1981 agcctacggt gaaagaattg tttctaggat atttaagaat tcagaaggtt atgcgctgaa
     2041 attccaacaa acagcagaca agcgcataaa accagtgaaa ggtagtatcg catgaataaa
     2101 taccgtaata aaaaaactgt tcatcgaggt atcaagtttg attctatcgc agaagcagag
     2161 tattacgatc tagccttgtg gcaagctgaa gcgaacggct ggaaagtaaa acttcaggaa
     2221 agatttgagc tgatgccgaa atttgaacta gacggaaaga agtatcgcaa gatcgagtat
     2281 attcccgact tcacatttta taaaaacggc aaactagtca aagtcgtaga tgttaaaggg
     2341 atgcagacaa aagatttcaa aatcaaagcg aaactcttct gcagtcaata tcgagtacca
     2401 ctgatcttag cgaagaaata tcggaatacg ttcaaggaag aacggtttta acgaggtgat
     2461 ccatcatgac aacagaagaa gtgattcaaa tgcgtattcg aagccttcag cgtgagattg
     2521 acgatctgta acggacaaag gcagtgatgg tcaatgaaac ggcgaggaag gcaatcgatt
     2581 tgcacataga aaatttaaga aggaaaatcc atcgattgga ggaatgagcg tggataagaa
     2641 agcagcaatg aaacgaatca tcgaactgac acattctgag aattggcaag aagacaaaga
     2701 aatagttgca gaagtccaaa agctcggtaa atcaatgtgg gctgaaaagc ctaaacggaa
     2761 aacgccgaga aaaattgcaa tctggcatgg tgatcgaatt ctagtaacag gtactgctga
     2821 acagttatct gaaattactg gattaagcaa aaacattatc tgggatagag ctaggagctt
     2881 atggattgat tcaaaaggac gacagtttag gtatgtggag gagaaataat gaacgaacta
     2941 atcacaaaag tagagcagtg ggctaaagac aagggactgg atcaagcgga tccaaaagca
     3001 caatttttga aagtagctga ggaattcgga gaaattgcat cggcaatggc aagaagtaat
     3061 gatgagctat ttaaagatag cgtaggagac gttatcgtca cgctgattat tctttccatg
     3121 caaaaagggg caaacataca agagtgttta gaaatggcgt ataacgaaat caaaggacgc
     3181 acaggaaaaa tggtagatgg tgtattcgtg aagtcgagtg atttggagga cagcgaatga
     3241 tgatgccaaa agaaatctat tcagatgcat taagaggtgc tagaaatcaa ttgaaaatgg
     3301 caaaaagagc atacgaaatt cggcccacaa tcgaaaatga acgcagagta aaagcaattc
     3361 gccgtagatg ttcaatttac ggcgagttac agaaggagga cagcaaatga taccgagatt
     3421 tcgagcttgg gataaacgaa agaacgtaat gagagatgta gccgtcttgc attttactaa
     3481 aaacggcaaa acaaacttta ttgaatattg gataaatcct accgaattga aatcatatca
     3541 cgtgcgaaac atcgagctca tgcaatcaac agggctgaaa gataagaatg gcattgagat
     3601 tttcgagtgg gatattgtat cagtcagcgt gcgaaatgga ttcgattatt tagataataa
     3661 agtttgtgtt gtcaaaaatt caataggaca ttcgggatta gtgtgtgcca ctgttgatga
     3721 agatttagag tatcaaattt ttaacacaga gctgtttgaa gaatacacgt atgaagtcat
     3781 cggaaatatt tgggagaata gcgagttatt ggaggaacag tgatgaataa acaggaattg
     3841 attgatgaat tagctaaata tgtaaagcgt tatgagaacg ttatggatga gcatggccaa
     3901 ggaaggtacg gcgcttatga agtatcttta aagttggtga aaagactaaa tgaatcaaaa
     3961 attacggacg aacaagcttg gaataaggta gctgaggctt atcctgaatc ggcacaaagc
     4021 ttgagaaaca ctttagataa tgctgtattt ggtaagactg gtgaaccgca gaagcacgtt
     4081 atgccgaagt ttgtggcgga gtggatagaa tacgctaaaa agaaaggtga tagtctagct
     4141 atttcattca agccgtggaa cctctatggt gttgagtata gcaaggctga tagatggatt
     4201 ggagataatc aagaaacgtt tgctcgtgct tggctagacg gctacgaggt cgagaaagag
     4261 ccgttgtatt atgtcccgct accatatgaa gtttgggatg aagaggcagc ggagcttaaa
     4321 acagagtatt tgtatctaca ttatgagatc acaagtgatg aaactagaat atttcctaca
     4381 aaagaaccta gaaaaggttt tgtcgcaaaa ttagatgaac ttacgattaa atcagcagat
     4441 gaaaactact ggccgtttgc tgtaccagtg gaagaggtgg tagaaggatg aaaaaattgc
     4501 accaactcat atctgagaaa gaaagcgaac tccaaaattt agaagatagt ttgggattgg
     4561 ggtttccaat tgttgagcaa gttaaaatgg ttcaaatcag tcatctacaa cttgaattgg
     4621 aagatttaag acaaatagaa gatccgtatc aactcaacga taaccagcaa atcgtgttgg
     4681 agtggttgaa actaactgcg tcaacaggaa agccaatgca agttgtattt tggatgatga
     4741 ataatgcagc atgggggcat ttagacgaat tgagggatcc tttgatggaa ttaactgaca
     4801 aacagcaatt cgaagtccta acagcattcg ctcaatgggg attagaacag gaggaaaaag
     4861 aatgaaacta aaagacggat tttacgctag tagccacggt atcggcggtt taatgctaga
     4921 catgccgaca aagaacccta aaacacgtaa gaagccaaaa gtcaaagtcg gtgacatggt
     4981 tcgctgtgaa gcagaaggat tcatctatcc atttcgtgga tatgtagaac acgtctataa
     5041 tcactcagcg atcattcgca ttgaaaacac gatggaatgc gacaagttat tagcgaaaag
     5101 caaagataat ttagcagtgg tgagattggt ggatatggag gttataaaca atgaaattta
     5161 aaatctttga agaggacact cgctataaat tagaaaaaaa attaaatgaa tttgcaaaga
     5221 ataatgaaat tcagcatata tctttaacgg cttctaagtc aggttataca acttactatg
     5281 cagctgttgt gagctacgta attcaataaa aggaactcgg caaataaaaa agccggatca
     5341 ctccgactga tgtaataaat ccgacaagtt tattatatca cataaaggag cggtttgact
     5401 tgatgcaatt gttacgagag gtagatttca aacagacaag atgtaatgcg agagatgtgc
     5461 tgaagaactt tcggcgcttg gagcggatgg caggtcgctc tttgatagat attaagtcgc
     5521 cgattattac ggatatgccg aaggcaccga agcacggcaa taaggcagaa gacgcgatca
     5581 ttcagatgat ggatatagaa gcggagagag acgcgatttt agcggctttg atggcgctta
     5641 gtctgattag ccgtcagata ctctactaca gcttctgtga cgtaaacaag cactctaatt
     5701 atgaaatagg gcaattgata cgaggatacg gagagaagaa tgtagagaag ctgaaatcca
     5761 tcgcattgat cgaatttgct gaagcataca aaaaaggcgt gttagttcag tatcgttaat
     5821 tttgtagggt ttttgtaggg atagtgtagg gtttttgagc ggtttaacgt gatattatga
     5881 tagtgtcgaa agattagtga taggtctaag acaaaataat aataaaagga acatcgtttt
     5941 attattgttt cacaattaag cttcgataga cagcagcgga aatattaaga ataaggatgt
     6001 ga
//
LOCUS       DAYYCY010000145         5894 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000145 DAYYCY010000000
VERSION     DAYYCY010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5894
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.146"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            124..2031
                     /locus_tag="ACHAZB_002289"
     CDS             124..2031
                     /locus_tag="ACHAZB_002289"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HFM8614920.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(2572..5850)
                     /locus_tag="ACHAZB_002290"
     CDS             complement(2572..5850)
                     /locus_tag="ACHAZB_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HFM8614921.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEVPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLESYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
BASE COUNT         1691 a         1218 c         1124 g         1861 t
ORIGIN      
        1 tgaaggattg aaaaaaacaa atcagtttga ttgttcgaat tttcaatttt ttatgttcta
       61 tttgttaatt ccttcatttt gtaagataat gggcacacga aacaaaaaag gagcgatcga
      121 tgaatgaaca aagaacgatt gaaacaagat ttctatgaag cagtcaatga agaatggcaa
      181 aaaacagcaa aaatccctgc agataaacca gctacaggcg gtttccaaga tttagttgac
      241 gggatcgacc aacttttgat gggagaaaca gacgatctgc ttgcccatcc agaaaaagtg
      301 tcttcagaaa aaatgaaaca tttccttgca tattatcaac tggcaaacga ttatgaccga
      361 agaaatgagt tgcatgctca accgcttcaa cctataatcg aaaaagttga atcattgcag
      421 tctttcgacg aattgaataa gcaatttcca gaatggctgt tggatacatt accattgcca
      481 tttaatctgg atgtagatgc agatatgaag aatgcgcaaa cgaacgcctt ctttgcttat
      541 ccgccttctt tattccttcc agataagacg tattatgcat cagaccatcc caacggtgca
      601 caactaatag gcgttttctt tgatatggct gttcagttat tagagcttgc cggcaaagaa
      661 aaaagtcagg cagaagcaat cacggaacaa gcagtgcaat ttgataaatt gattgctcct
      721 catgtaaaaa gtgcagaaga aaacgcagat tacagcaaaa tgtataatcc gcaatctttt
      781 gatacattta ttaaatacac agataaactg gacttgaaaa aacttgtcac gggtttgatt
      841 gggacaactc cagataaagt gatcgtaact gatccagtgt acttcgatca tttgaatgac
      901 atcctgacag aagctcattt tccgttattc aaaagctgga tgatcattaa aacagtacgt
      961 tctcttagtg gttatctatc agaagaattc cgccaagtca gcggaatcta ttctcgcgta
     1021 ttgtctggaa cagacgaagc aatgcctcag aaaaaagcag cgtattacct tgcctctgga
     1081 caattcgatc aagtagttgg cgattactac ggaaaaaaat attttggcga agctgctaaa
     1141 aaagacgtag aacaaatggt gaaaaaaatg atcgccgttt ataaaaaacg tctagaagaa
     1201 aatgactggc ttggggatgc aacaaaagaa aaagccgtca taaaattaaa taacttaggt
     1261 atccaagtag gctaccccga taagatccca gcattattcg accagttcaa aacagtacct
     1321 gcagatcagg gtggtacttt gctttcaaat actcttgttt tcagcaaact cacattaaaa
     1381 gatcgtttca gcaaatggaa caaaccagtg gatcgttcag aatgggaaat gagtgctgac
     1441 acggtcaatg cttattatca tccatttcgg aatgtgatcg tctttccggc agcgatcctt
     1501 caagcaccat tttacagtct agaacaatca agctctgcta attatggtgg aatcggagct
     1561 gttatcgctc atgagatttc ccatgcattt gataataacg gcgctttatt tgacgaatat
     1621 ggcaacttga acaactggtg gacagaagaa gatcttacac aatttcaaaa caaagcacaa
     1681 gcaatgatcg aagaattcga tggtctgcct tttgctgacg gaaaagtaaa cggaaaatta
     1741 accgtttcgg aaaatattgc tgatgcaggc ggtctaagct gtgcattgga agcagcaaaa
     1801 acggaatcag atctttctat cgaggaattc tttgtcaatt gggcaacgat ttggcgtaca
     1861 aaagcaaaaa aagaatacca acaactttta ctgcaaatcg atgtccatgc ccctgctaaa
     1921 ctccgagcaa acgtgcaacc aaaaaacctt gacgaattct atgaaacatt tggtatcacg
     1981 gaacaagacg ctatgtatct agcgccagaa aaaagagtgc gaatctggta agaggttgtg
     2041 ccagaagtgg tcagcttcaa gaaataagaa ggaatttggg aaaatcgctt ctcaaatttt
     2101 tgtgacattt cggcttattt ttcgaagaag ttacttctgg aataccatgt attagaggtt
     2161 gtgaaaaagc tgcttagttt caagtagtaa gaaaaatttt gtgaaaatgg ctttccacat
     2221 ttttgcataa atttgactta ctaccgagaa actggctttt gaacaccgtt tatcgagagg
     2281 ttgtgaaatc agcgtcctga cctgagtatt agagaaaaaa acggaaaaat agttcctcat
     2341 attttgccgt ttgttgagct aataccgagg gtcagctgat tgaacaccgt ttagtggaga
     2401 gattgtgaaa aagctgtcct gcatcaagta ataagaacag ccaatcaaaa gcagcaagga
     2461 gaacctcatc tataaaaaga tagaaggaag tgagacatct attggtctca tttccttcta
     2521 tttttacttt cctcagtcaa ttgttttaca tcataaaagg agaaattcca cttatttttt
     2581 tcgctgtttc atgacataaa caactacacc gattaacaaa attcctccag ctaatgctag
     2641 caacgggtta ttaaattctc ccgtttttgg gagtccttga gtgccatttt tttcactttc
     2701 ttttgctgtc ctatccggat gagaaggttt tccatttggt tttccatttg gtgattcatt
     2761 tcctggattt tctctttctg gtttgtcatt tcccgggggc acatcggcag tttctcgcac
     2821 agtgatcgct ttttgctggg taacagattc tgtatgagca gaccattttt ccccatcaaa
     2881 tatttccgaa cgataagtga cttctagcgt atattctcct tttgaaggtg ctgaaaaaat
     2941 agcggtatga ttagaaaatt gagcacttga ctgtgtttca acgatttgga tcttttctgg
     3001 gaaataacgc atatcttcaa agacaggcgt tccttgattt ggtgtgaaag ttgacacaaa
     3061 aactgtaaaa ggcttcagtt caatgatatt tccttctgca ttctccgcca caccatttgc
     3121 ttcttgtata ataatcggat tattttgttc atttggtttt gtctgatttg gttcttgtac
     3181 gattgcatac tctggatatt ccgaagacgg tgaggtttct ccgtcaaaaa tagcacctac
     3241 agataatgga agaccatcca ctctttcctt ttctgtttga cgattccccg gtttaatctg
     3301 catcagtcca tgaacatgga tacttccatt ttcatagcgc gtaacaggct gctgaccttc
     3361 tttcgaacat gtatttagag actcgatcca atcttttgat atggtatgtc caagagtatt
     3421 ttctgattgt ttctctcgtt cattccaata gtagttcaac gagtggtaga tttgatcttc
     3481 gttttgattc aatgggcgga tctgctctct catctcaaga ttctctgttc ggaaagataa
     3541 gataccagaa gcatggtagt ctgtgtttcc tgctgaagag gtataaagag ccacattata
     3601 tgaaccgtta ttaaaacttg tactgctatt gacgataata tctgttccgc tattggaatc
     3661 gattcctttc gcaagatttt cgaaagacaa actattgatc aattcatgag ggcccgttaa
     3721 agaagaaccg cccattttga atccattacc gttaccttct ccctcaattc ctggtaccca
     3781 gccgtttcga taagcaactg agtttttgat gattactttt ccgatcgaac ctgtttcatt
     3841 tttagcaaaa aggtcccaac cgtcatctgc attatgatac gcgatacaac cgtcaaatac
     3901 atttccttct cctattgtca gtttggccgc aaatccatcg gcatcttcaa atcctggatc
     3961 ggcattttca aaagatgtac agttcttgac taagttatgc gcgggccatg cttcgaaagg
     4021 atcatttgag ctaccgctga tttggattcc tgtatttcca ttccgataag catgaatctc
     4081 ttcgatcaaa ttatgatttc cagaaatctg taatcctttt tgcatagctg cactatttgt
     4141 aatgtcaaac cctcgtaaac cccagtagtg actgaacagt gtcatcccat ttccagttcc
     4201 tttaaaatca agtacaggac gcttgttttc tggatttttc tctgcaatca actgaatcgg
     4261 gttttctctt gtcccgttaa cgccttttgg aatcgtcaat cctctttcga aggaataagt
     4321 tccaggcgcc atgacgattg tctgcccagc atatgcgtat ctcaatgctt gtgtcagaga
     4381 taatggattt tgtctgcttc cgtttcctgc atctgaccct tctggtgtta cataaactgt
     4441 cgaatattta ggtttacggt agtccactgt atgaagaatc tcttttgttt cataactagt
     4501 catctctacg tattcttctg gcggatagtt tttatctggc gtaaaactga aagtaaattc
     4561 gttcgttcct tccatcagtt ttgtctgata atcaaattcc cgattcgcag tgatagccaa
     4621 gttttttgcg ttcttaagca cctctcctgt tgctttattt ttaattgtca ataatccgtc
     4681 agcatttgct ctaaaggaca tagtatgatc ttggtttcct gaatgattac tagaggtcac
     4741 agtgtaaatc ggatcaatca agcgagtagg tctttcttgc ccaggaggat ctgtcgcagg
     4801 atctgagtaa gcaacttcga ttccttctac cttgatcgtc atattccttg ctgcagcaaa
     4861 tcctgcataa ataaccgatt catctgaagc gtataatttt tcccagtcat acatgatgtc
     4921 agaagccatt tcttctcctg tttcaggatc gtaataatat gactctaaac cagtattcgt
     4981 ttttttcatc tctacgtcca gtttagtaat cgagttcgtg gccggaaaca aattcccatg
     5041 accgtttccg aatatattat aagaacctgt ttctttttcc aagaaccctg ctgaagtctc
     5101 taaaggtgtt gtttccactg agacactccc aggagctggc gcagcttgcg gatcttcaga
     5161 tgatattcct gtgattgccc gtgtccccaa tccaagtcgc atcgtatatt tgctgccgct
     5221 cgtgttgacc acttcttgtc tatccgagtc ccagacatat tcgatccgac tgcctagtat
     5281 ggcaaatgaa tttgaataaa atgcctgccc aaacaaatga ttttttggta ctgcgtctct
     5341 taccattaaa gcaaatcctt cttgggcatt cgtataggtc caagattcta tttcgatcgt
     5401 cgcccgcatc ctaaagttga tgtctttggg gattgcttga taataatagg tcattccatc
     5461 tgatccgctt gactggaatt ttccgccgtt attcgtggaa gagagccgca cgccgtcttc
     5521 taatggttca atggcatacc tactagcact tgtgctttct ccgaaaacaa cccctttcca
     5581 ctcttcattt gcttcttcag attgcttttt tgtcttttca ttttctgatt ctttcatcat
     5641 aggatcattt ccttcttccg tatccatatc ttcactagtt cttggtactt cctccattgt
     5701 tgacatccct tcaatgttca cgtgtgcatt ttcttcgttc acagactgtt gttcgttagc
     5761 agcaacttca acgacagaaa ttaaaaccgg ggtactgagc ataaggagat tcatcattag
     5821 cgcactgtat tttatacgtt tttttaacaa gtgtattcct cccgtaacaa attagaaagc
     5881 gtttacttaa agta
//
LOCUS       DAYYCY010000146         5880 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000146 DAYYCY010000000
VERSION     DAYYCY010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5880
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.147"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(106..564)
                     /locus_tag="ACHAZB_002291"
     CDS             complement(106..564)
                     /locus_tag="ACHAZB_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFM8614922.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(577..1908)
                     /locus_tag="ACHAZB_002292"
     CDS             complement(577..1908)
                     /locus_tag="ACHAZB_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HFM8614923.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(1919..3361)
                     /locus_tag="ACHAZB_002293"
     CDS             complement(1919..3361)
                     /locus_tag="ACHAZB_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8614924.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(3348..4112)
                     /locus_tag="ACHAZB_002294"
     CDS             complement(3348..4112)
                     /locus_tag="ACHAZB_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HFM8614925.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(4216..5091)
                     /locus_tag="ACHAZB_002295"
     CDS             complement(4216..5091)
                     /locus_tag="ACHAZB_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM8614926.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(5346..5430)
                     /locus_tag="ACHAZB_002296"
     tRNA            complement(5346..5430)
                     /locus_tag="ACHAZB_002296"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5394..5396),aa:Leu,seq:caa)
     gene            complement(5448..5519)
                     /locus_tag="ACHAZB_002297"
     tRNA            complement(5448..5519)
                     /locus_tag="ACHAZB_002297"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5485..5487),aa:Gln,seq:ttg)
     gene            complement(5529..5601)
                     /locus_tag="ACHAZB_002298"
     tRNA            complement(5529..5601)
                     /locus_tag="ACHAZB_002298"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5566..5568),aa:His,seq:gtg)
     gene            complement(5663..5747)
                     /locus_tag="ACHAZB_002299"
     tRNA            complement(5663..5747)
                     /locus_tag="ACHAZB_002299"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5711..5713),aa:Leu,seq:caa)
     gene            complement(5768..5838)
                     /locus_tag="ACHAZB_002300"
     tRNA            complement(5768..5838)
                     /locus_tag="ACHAZB_002300"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5804..5806),aa:Cys,seq:gca)
BASE COUNT         1795 a         1253 c          896 g         1936 t
ORIGIN      
        1 acaaaaatca aaatgattaa atctagtgat tgaaacatat catttcaaaa aaattttgta
       61 aattcaccca taaatatcac cctatttaac ttttttcatc gtacactaaa attcaaaaca
      121 actatcttta atctttactt cttggttttg ttttaataaa tttccttcaa aaatgcctgg
      181 agaaactaca atgatcaccg tatctttttg tgcttgctcc aacttcttca aatctataat
      241 agctaacagt gtcccttctt ttacctcctg gttactttca acccgtatct gaaacggact
      301 tccttcgagt tcaactgtat ctaaacccat atggatcagt atttttctgc cagattcact
      361 ttcgatcgtt atggcatgtc gggtaggaaa tatattatcg attattcctt taactggcgc
      421 atggatatat ccatcatgat tcgttatagc gtatccaact cctatcattt ttgaagaaaa
      481 gacctcgtct tttactgctg ttaatggaat aatttgacca ctagctacag cttttagaat
      541 gtttcttgtt ttaaagaaat gcacttttta tcacctctac tttaatgctg gccaatattt
      601 tttgtttact ttgatcattt catctagtat tttttttgcg actctggcat ttggtacggt
      661 ttgattcaat gtaaatgctt gtaaagcttt ttgataagaa ccttcaaagt aagcatctac
      721 tagtaacttc tctactgcaa cttgtgcctc catcaatcct ttatgaaaat cactgattgg
      781 ttttcttagt gtaattggct ccacaccagt tgcaccaaca tatgccggta tttcaacaac
      841 tgcatctgac cgtagattcg gaatcgcccc ttgattagga acgattagca taaagcgacg
      901 acgatcatca ttcaacaagg aaatagctat atcgacgata tattgtccat gctccccaaa
      961 attaaagttc aatacttctt ccacaatacc gcggcgtatt ttatcagcca tttctttggt
     1021 atttttctct cgtccatcca tgaccatatt agcacgtgta tactctttgt cagtatgctc
     1081 aacgaccata tcaggatata agtaatactc taagtaatta ttcgggatat tacttggaaa
     1141 atttttcgtg atgactgaca tcatttgaaa ggttttttgc caagttttat ctcctatatt
     1201 aaaatcggcg acttgcattt cttttgttat tagtttttca ataatctctg gcataatttc
     1261 tcgttgcaat tctttatcat aaatcgatgt ataccagcca aagtggttta aaccataata
     1321 cgtaacgatc caattcttac gatcataacc gtaattcact gcaatcgttt cttcaattga
     1381 aatagtcata tcacaggcat taatcatttt tacattcgga tatttacgac gaacagcttc
     1441 agaaacgatc gactctggat ttgtataatt caaaatccat gctttggggg catagtcttg
     1501 tatatgatcg acaatttcca ataaaccttt gattgagcgc atcccataag caaatcctcc
     1561 gagtccacac gtttcttgtc ctactaatcc atgtttaagt ggaatctttt cgtccttttc
     1621 tcgcatctcc aatcctccaa cacgaatttg tgagaaaaca aaatcgcatc ctgtaaaagc
     1681 aagctttgga tcatccgttg cacgaatgcg aatatttgaa aaaccttttc ttttcagcat
     1741 aaatttaaca atcaagtaca tatccatatt tctttcttca tcaatatcat ataaacgtat
     1801 ttcagataat ggcaaccgtt tttcattgtt caaaatggct tgaacgattc ctggtgtata
     1861 cgtactcccg cctccagcta tagtgataat ctgttttttc atcatcatct tttcctttct
     1921 attcttctaa taatttattg aattctgcca taacataagt cacttctaag ccgatgataa
     1981 tttgataatt tttttcagtt ggtttaatga tcccgcttat ccctaattgc ttgattcgct
     2041 gttcctcgac catggtggga tccactacct ctacccgtag gcgtgtatta caactataga
     2101 catccaggat attggctttt cctccaaacg cttgtagcat ttttgttgcc atatattcat
     2161 aatttttact tgctaaccct aactctgttt cttctaaggt tacttcattg ccaaactctg
     2221 ccttagcttc acgtcctaat gtctgaatgt ttctcttatt gatcaaataa taaaaagtaa
     2281 cgtaatacaa ggcaaaaaag aaaattccga ttggaagaat caaaatagca ttggtagcga
     2341 ttcgaaaatt gatgagataa tcaacaatac atgctccgaa tgtaaaacca attcgtatat
     2401 tgaataagta acagacaatc cctgctaaac cggcaaaaag tgcatggatc acgtacaaca
     2461 taggagaaag aaacatgaaa ctaaattcaa caggctcagt gatatttgca ataaatgaag
     2521 ttcctgcacc gcttcccatg attcctttga ccatctcttt attttcttta taagctgctt
     2581 tatagatagc tagacatata gctggtatac caaacatcat tactacaaaa aaaactgata
     2641 aaaacagtcc agcctgagga tctccgttga taaagcgtgt catctctcct ctgaaaactt
     2701 ccttgctagg agatgtatag cttcctaaat cgtaataaat atacgtattc aacacatggt
     2761 gcagtccaaa ggggatcagc aaacgattca aaaacataaa tatccccaca cctaatgctc
     2821 ccatatcaac gagcagtttt gcaaaattat caatcccaat ttggatgagc ggccagataa
     2881 agcccaaaaa aacagctgcg attgtttgta gaatcatcac catagtcaat gggaattttt
     2941 cgcccgcaaa ataagaaaaa actactggta atttttgttc tttaaagcga ttatacgtcc
     3001 atgccgctac taaaccggaa ataattccac caaaaatccc catattcact tctgggtcca
     3061 taatttctaa gccttttttc aaggtagcaa tcgctaaaaa accagtaagt gcaggtattc
     3121 ctttgtcctt atttttgaca aaaccgattg caactcctat agcaaataag atatccaagt
     3181 ttccaaaaac ggcatttcca gctgcaccaa ttactgggat gttcagcata tcgtcagctg
     3241 ataaacgcat gatcaatccc gctaatggca tcgctgcaac tggaagtagc atcgctcttc
     3301 caagcttctg cacatttgat ttaacattca tttttattcc tcctctatta aatatcctca
     3361 tatgttacta actcaatcaa aggatcattt ttcacttttt gagcaaatgc tgaatgaatt
     3421 aaaaattcga cttggtgggt acgaggatac acaaaacttg aaccatttaa caattcctga
     3481 tccaaatacg ctgcgtgaca catgacttcg atcgttttat cttctctcaa tatcatgagt
     3541 aaacttgcca aatattcttc cacactcgta ttgatttgtc gttttttttc atcagtttct
     3601 cctacgacat cgaaacaagg ctcaaaataa tcgacatgac gaacttcatc ttttcgttca
     3661 aagttgctcc gaaccggcaa atcatatttt tttgctaatg taacaactac ctcctgattt
     3721 tcatccaaag tatgagtatg atgatgactg tctaaatgag ttggtatgat ccccgcatga
     3781 taaatttttt gaatctgagc attccattcc tgatacagtt gatctttgtc aatgttaaat
     3841 ggatgctgat aataagataa ggatctgaac tctccgcttt cgataagagt atcgacagtt
     3901 ttgaggagtg gtttgccaca cgtcaatgtt aaatggactc ctacacctag cctaggcatt
     3961 tcctttctta gtttcacagc atgctcgaaa cccggcatat ttgccatcaa tgtagttgat
     4021 gtcaaaattc ctcgctgata agcatccata atcccataat tcacaccatg gctatagcca
     4081 aaatcatccg aattaaaaat cacttttccc atttcattct ccttcttttt tctactaaaa
     4141 taggcgcaac caaataatca tagtccattt actatgtgtc atttggttgc gcctatagat
     4201 agttacgccc ttattttaat agattcttgc tagatgaatt gccaaataga tgatttcatt
     4261 ttcttgaatg gcaagttgaa attgctgttc gatgatatct ttaatcttta gtgctgtaca
     4321 atacgctttg ctatgtttaa atgtttcagt aaaaaaacca atattttcta ctggcgtttc
     4381 ttcctgataa atcaagcgtt taaccaggaa tttgatatga accattaagc gatgataagt
     4441 cagtgattct ttatcataaa cgtttgaata ggatttttct agatgggaca aaatcgtggt
     4501 cgtcatttct actattttta agctctttaa actattaggc tctcctagct cagcattaat
     4561 gatatgcata gtgatgaatc ctacttcatt tgaccctaac tcggtttgat atcgatgatt
     4621 gattcgctcg accacttcta ctgctatatc atattcattt ggataaagga atctcatttc
     4681 tggcaacata attgctgtga catctctatc ttctcttttt cgttgaatag caaaataaat
     4741 atgatctgtc aatgtaatat agatatttga atctaatgga gaggagatcc tagctgttat
     4801 cagttcaatc acttcttccg caaaatccat aaattcgatt ggaatctgcg agactatctc
     4861 actgaatcgt ttgatctttt ctttagaatc taatacaaat aatttgtcag catcctcaac
     4921 atcaaatggc ttactcactt tagcagaaaa gccaatccct ttccccatcg caattttttc
     4981 tattccatcg tcatcgatca atacagcatt atgattcagc tttttcatca catacaccgt
     5041 taattcacct cctctatatg ataaaacaaa acaacttgca gactccttca tacatatttt
     5101 tgtctccaaa caatttttaa agacaaaaaa cgacctaaga aagaaccctt tcttaggtcg
     5161 tgcctaattg aatagtagca ccctacataa tttacaaaaa tagtttaaca cataataaaa
     5221 gcgttatcaa tatctttttt actcgtttta tgataatact tgtatcgaat tgatttggag
     5281 gtaattacat aaaaacaaaa aaaatatagc aactctctat aagaaagttg ctatattgat
     5341 aattataccg gcggccgggg tcgaaccggc acgtcctcaa cggacactgg attttgagtc
     5401 cagcgcgtct gccaattccg ccacgccggc aaatttaaaa ataataactg gggtagctgg
     5461 attcgaacca acgcatgagg gagtcaaagt cccttgcctt accgcttggc tataccccaa
     5521 gaataaaggg cgaatgatgg gaatcgaacc cacgaatgcc ggagccacaa tccggtgcgt
     5581 taaccacttc gccacattcg ccatcataaa agatgttatt taatttctca ataaaaaact
     5641 aagatgaaga catcttagtc aataccggcg gccggggtcg aaccggcacg ccctcaacgg
     5701 gcactggatt ttgagtccag cgcgtctgcc aattccgcca cgccggcaag ttgatttatt
     5761 agtactaagg cggtaaccgg atttgaaccg gtgatgaagg ttttgcagac ctctgcctta
     5821 ccacttggct ataccgccat tatactatta tcaatctata aaataaagaa taaattatag
//
LOCUS       DAYYCY010000147         5809 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000147 DAYYCY010000000
VERSION     DAYYCY010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.148"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            450..1604
                     /locus_tag="ACHAZB_002301"
     CDS             450..1604
                     /locus_tag="ACHAZB_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C47 family peptidase"
                     /protein_id="HFM8614927.1"
                     /translation="MIILKPKNKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYVYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNG
                     QIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDN
                     FIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKS
                     LNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALAL
                     FGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            1577..2020
                     /locus_tag="ACHAZB_002302"
     CDS             1577..2020
                     /locus_tag="ACHAZB_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8614928.1"
                     /translation="MGQYHYKLVNKPIVLVMGLVLVLFLTSCSKKQEIQKTELIPFLG
                     TWQTKDHSERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNL
                     LTLRSNKKQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            3143..4075
                     /locus_tag="ACHAZB_002303"
     CDS             3143..4075
                     /locus_tag="ACHAZB_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFM8614929.1"
                     /translation="MANEIVKYKPELNTIPLRKFTPIEMNLFFSIVSRMRDKGNQKIR
                     FYFDQLKDLSAYKQTAGEVFIKDLTRTYDHLMDLRFGKKSNGGLTIERFTMFTDFEIH
                     GEADNPYVDIKVHEKALPLLNNLESWVRFSLTEFRDLNSSYAKTMFRLLKQYRTQGWA
                     EFSKEDFLELLDIPKSYRQTNINQFVLKPIKEELTPLFKGLTIRKKYGKGRGKPVIGY
                     RFTWKAEINHADDFSKGKQEDLRTKLFNIEHNGELTQEEKWRAKDRILDLPLGTHEAN
                     FKKQQQTEKEEVEKQTLSNELKQDLLGNLQNLFD"
     gene            4092..4607
                     /locus_tag="ACHAZB_002304"
     CDS             4092..4607
                     /locus_tag="ACHAZB_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFM8614930.1"
                     /translation="MADKTIKDLAEELGVSKTAINKKVSDENRKLWFAKIGNKFVINE
                     IGQKAIKSMFFTENENSNQQPVGEKSKTENHKNANQVSELELFFFLEKQLNQKDIQLQ
                     EKDEQLKQMQKLLDQQQQLTLQANQQIKKLHEQLQLTYAEESSEKEPLSVTEETQKGQ
                     NKEKKWWQFWQ"
     gene            4903..5184
                     /locus_tag="ACHAZB_002305"
     CDS             4903..5184
                     /locus_tag="ACHAZB_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000353845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8614931.1"
                     /translation="MDILVRNINPKIITRVDKRCQELTTKTGEKWSRNKYLKVLIESD
                     FDRQLMNYKKEQFDLLLEKFIDIQTKNTETLNDYVHTNNQLIETLIGGI"
     gene            5186..5602
                     /locus_tag="ACHAZB_002306"
     CDS             5186..5602
                     /locus_tag="ACHAZB_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8614932.1"
                     /translation="MRKTSVQKHVTLYPSHIDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EKQLNPSQRNQPKNRQKETSQTFTSKNFDPTNFLKR"
BASE COUNT         2229 a          960 c          930 g         1690 t
ORIGIN      
        1 aaaaaaaaag actgctcgaa agagcagtct caagcaaaaa ataaacgaac aaacgcaatt
       61 actcacgaac acaaacaaat acaataaaat acctaaataa tgaaaataag tgataaaaaa
      121 caagggtatt tatagtttaa caagcgctca taaaaataac aagtctttaa aaaatgacag
      181 ataaaaatgc acaaagaatc ggacacagat aaccgcgaaa aatcaagctt tagaaagttg
      241 tttttagcta gaccaatgaa aaatcacaaa gtccgcccct gtatccgttg gtagaggggc
      301 tacttggttt agtatacatt atgttaacta gctcgttaaa taacgattat gttcccaaaa
      361 caatatttta tgaactataa caaaaatttg ttatcgcata ttgtatattt taattatagg
      421 tgtatatcac ttaattttaa cacaaaggcg tgattatttt gaaacccaaa aataaaattt
      481 atctaggtct cctcttttcc ttgatgattg gaatttttag tataggaatt acaaatacct
      541 atgcttcaga aaattctaat ttcaatatca actcccaacc aattgaagaa caagcaatta
      601 attttgcgaa aactgatttt gaacactcca taacttcaat tcttttaatg gaaaataaca
      661 ctgaaactga tattaactat actcttggtt cgccatttaa aatgctcgat ggagaaaata
      721 cggcaaatag ttctattttc tttccagtat tttcagacgc tgataatacg attaaatatg
      781 tttataccgt caaaaaaaat acaaatggag aatattcagg aagtatttca caatttttag
      841 caaaagaact tcaaaatcta gtagatagca atataacaga tacaacctta ttttttcaag
      901 ataatagcat ttattattcc gataatggcc aaattaaact actttggaaa tcacctttaa
      961 gtacagaaaa aaatggtgtc tcttccttta cagaaaaaaa tgatcttata tccgtaaata
     1021 taaaaaataa aattaaaaat ttctctaaaa aacaaaattt tcctgcggtt aaacgtgtaa
     1081 aaagtgtctc ctccggagat aactttatta tgattaattg gcgtatagca gaaacccaaa
     1141 caagttttcc gtggtgcgct gcttacgtag aagctgctat cttatgtaat aaaacagatg
     1201 tacgcgctac ttcagctaga gccattatgc attataccta tcctaattta aacaacagtc
     1261 aaattgaatc aaaaagtctt aatcaagatc aaataataaa atatgcaaat tactgtggtg
     1321 tttatcctaa aaaagtagca cgaatactaa acctttcaga ggtccaaaca caactaagaa
     1381 aaggaaatgc catttatatg ggaactgctg gttatggaaa ttatagtaac agccgtcatg
     1441 cacttgcatt atttgggtgg gtcaatcaag gtggttatca aacttactat gtgtggaatc
     1501 cttggtataa ctacccaact gtaataagtg caaatgcagg gccagttaca attcctgtac
     1561 ctggaggtgg ttttacttgg gacaatacca ttacaaattg gtaaataaac ccattgtttt
     1621 agttatgggt ctagtgttag ttcttttctt aactagttgt tcaaaaaaac aagaaataca
     1681 aaagactgag ctgattcctt ttctaggaac ttggcaaact aaagatcact cagaacgtct
     1741 agtaatcact aatttaaata cttcaaaaaa tatagaacaa tataccatct tagtaaaaga
     1801 gaacaatcat aaaattcaaa tcattgataa aaatttaaat ataaatgaac aagttttcat
     1861 ttacaaagca acaaataatt tactaacact gcgctctaat aaaaaacaat tagattacac
     1921 ttttagtttg gattctccaa aaaagctcgt atttattttg acaacgaatg atgggacaga
     1981 agggacagct aaaccaattg aattttttaa agctgattga tgaatagttg tttgatatta
     2041 caaaacaata atttcaccga aggtgaaaca agcgaaactt tacaaaaagt ggctagcatt
     2101 tgctagcctt ttttgtattg cggagcgtga gttttgaggg ggattcaagg gggagtttct
     2161 cccctttgac aagcggttat ttgcaccaaa tacgaaggta tttggctagc aaatatagag
     2221 cttgccaaac cctttagtgt gttttgtaga aagggacctt atagtcactt tcacattaca
     2281 aacgatacgt cctatggacg tatcaaaagg gtgggactta tgagcaaatt aaagcaaaat
     2341 ttggctagca atccttctaa aaaatacacc tattgatcag agtcaaaata aatcagtttt
     2401 cgagtgagcg aatccgaatt tgcaaatctg aagatttgca aatgagtgtg ccggcttccg
     2461 taaaagtcta ggtaacatta gtcgatatat acataaggct ctaatttgcg ttttaaggcg
     2521 ttttaaacgt tctggtatgt aatttatacc aaagcgactt taaaacgcct taaaacgaat
     2581 tttataggtc ttgtaggacg tttcagggtt gttagggctt gccctgaccg cctgtaaggc
     2641 gctttaaaaa caagataagg gattttatct agacttgtct taaaacgccg tagatgagcg
     2701 ctcagagctt cttaaaggac tccttacgaa ctgtttttaa gcaaaggagc ctttaggcga
     2761 ctgcgcagtt caatgtgaga gcgtagcgat cacaccttta aagcgaccgt agggagcaga
     2821 ttcccagttt aggaaatttc gcgaacctgt ttggagaaaa aaagtaggaa atttttaggc
     2881 gggaaatttt gcccccctct tttataatct cttttataac ctctttttaa acctctttac
     2941 cgtcggctgt atccttactc tcccaaggcc tgtagctttt ttagggcatg aaattatctg
     3001 tttataggca tgaaattatc tgtttatagg catgaaatta tctgtttata ggcatgaaat
     3061 tatctgtttt aggtattgtt ttaagacata atatacctta aaattatgtc aataaaatta
     3121 gttagactgg agtatcaaat aaatggcaaa tgaaatcgta aaatataaac cagaattaaa
     3181 taccattcct ttaaggaaat ttactccaat agaaatgaac ttgttttttt ctattgtttc
     3241 tagaatgcgt gataaaggta atcaaaaaat acgcttttat ttcgatcagc ttaaagattt
     3301 aagtgcttat aagcaaactg ctggagaagt ctttataaaa gatttaacaa gaacatacga
     3361 tcatttaatg gatttacgtt ttggaaaaaa aagcaatggt ggattaacca tagaacgatt
     3421 tacaatgttt actgattttg aaattcatgg agaagccgat aacccttatg tggatataaa
     3481 agttcacgaa aaagctttgc ctttacttaa caatttagaa agttgggtac gtttctcttt
     3541 aacagaattt cgagatttaa atagcagtta tgctaaaaca atgtttagat tactaaaaca
     3601 ataccgaaca caaggatggg ctgaattttc caaggaagat tttttagaac tattagatat
     3661 tcctaaaagc tatagacaaa caaatataaa tcagtttgta ctcaaaccca taaaagaaga
     3721 actcacacca ttatttaaag gattaacgat tcgtaaaaaa tatggtaaag gccgaggcaa
     3781 acctgttatt ggttatcgtt ttacttggaa agctgaaatt aatcatgctg acgatttttc
     3841 aaaagggaag caagaagatt tacgtacaaa attatttaat attgaacaca atggagaatt
     3901 aacgcaagaa gaaaaatggc gcgctaaaga tcgaatacta gatcttccgt taggtactca
     3961 tgaagctaat tttaaaaaac aacaacaaac tgaaaaagaa gaagtggaaa aacaaacact
     4021 ttcaaacgaa cttaaacaag atttattagg aaatttacaa aacttattcg attaaagtat
     4081 aaggagtatt tatggcagat aaaacgatca aagaccttgc tgaagaatta ggagttagta
     4141 agacagctat taacaaaaaa gtttctgacg aaaatcgaaa actttggttt gcgaaaatcg
     4201 gaaacaagtt tgtgattaac gaaatcggtc aaaaagctat aaaatcaatg ttttttaccg
     4261 aaaacgaaaa ctcaaatcaa caaccagttg gcgaaaaatc gaaaaccgaa aaccataaaa
     4321 acgccaacca agtttccgaa ttagaactct tttttttcct agaaaaacaa ttaaatcaaa
     4381 aagatataca gctacaagaa aaagacgaac aattaaaaca aatgcaaaaa ttactcgacc
     4441 agcaacaaca actcacctta caagccaatc aacaaatcaa aaaacttcac gaacaactcc
     4501 agctaaccta tgcagaagaa tcttcagaaa aagaacccct ttctgttact gaagaaactc
     4561 aaaaaggaca aaacaaggag aaaaaatggt ggcaattctg gcaataaccc aacaattttt
     4621 attcataaaa attgttgggt tactttcttt ttttgctttt acaagcatag cgacatgatc
     4681 tcacgtcgct attttttaaa gagcataagg aaacgcggta tcactttctt acgaaaggtg
     4741 ctaggtaggc tgtgcctacc acgtttctaa gaaaaaaatt tgctatcact ttttcgccat
     4801 tgtggctagc ttatgtatag tatttcggta aaaaacagct atacaaatac tatatttgta
     4861 tgatatacat aaattataca cacataagaa aggacgtttt ctatggacat tttagttcga
     4921 aatatcaatc caaaaattat tacaagagta gataaacgtt gtcaggaact tacaaccaaa
     4981 acaggagaaa aatggagtcg aaataaatat ttaaaagtgc taatcgaaag tgattttgac
     5041 cgtcaactta tgaattacaa aaaagagcaa ttcgatttat tactcgaaaa atttatcgat
     5101 attcaaacaa aaaatactga aacattgaac gactatgttc atacaaacaa ccaactcatt
     5161 gaaacactta tcggaggaat ataacatgag aaaaacttct gttcaaaaac acgttaccct
     5221 ttatcccagt cacattgacg ttttagattc catcatcgaa aaaaatccag atatcaataa
     5281 ctactcggaa gccattcgct ttttatgtat gagttatgaa cctgatacca atacaacaac
     5341 taccagtaac caagccaaac taaatgcgat gggaaaagaa atttctatca tgaccgaaat
     5401 tatcgcagaa tttgctgatt tgtatttaaa agatacaggc gttcttatcg gtacaaactc
     5461 gaaagtatat caagaagtaa aagaaaaaat tgaaaaacag ctaaatccct cccaacgaaa
     5521 tcaaccaaag aatcgtcaaa aagaaacgag tcaaacattc acttctaaaa atttcgaccc
     5581 aaccaacttt ctaaaaagat aaaaaaataa accaggaaat ttttaatttc ctggtttatt
     5641 ttctagagag cggtcttcca ataagatttg caaatcgatc ccaatttgcg taaaaaaaaa
     5701 gctgctcgta cgagcagcta aacaagaaaa agtcagcttg ttttaagggt aaaataagtg
     5761 taacaaattt tgcgaatgca acgatctttt ttattatacg caaacaatc
//
LOCUS       DAYYCY010000148         5790 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000148 DAYYCY010000000
VERSION     DAYYCY010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.149"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(99..1229)
                     /locus_tag="ACHAZB_002307"
     CDS             complement(99..1229)
                     /locus_tag="ACHAZB_002307"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFM8614933.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1288..1515)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1877..3079)
                     /locus_tag="ACHAZB_002308"
     CDS             complement(1877..3079)
                     /locus_tag="ACHAZB_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8614934.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3139..3342)
                     /locus_tag="ACHAZB_002309"
     CDS             complement(3139..3342)
                     /locus_tag="ACHAZB_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFM8614935.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3484..4551)
                     /locus_tag="ACHAZB_002310"
     CDS             complement(3484..4551)
                     /locus_tag="ACHAZB_002310"
                     /inference="COORDINATES: protein motif:HMM:NF014537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFM8614936.1"
                     /translation="MLDSLQEEVVPVNRVLIDYLTIHFFSNQHLKLIEEIIEMPIERF
                     EELDYAPKGYIGQYVWNQVITIRYSIDDAVKGTVMEFSGQGCKHLARWLKTRKTNWQQ
                     FFRKVLDYQGNFTRIDFTLDDFVGSLSIPELKHKVILGHVWTTFQVSESHGGTDIINN
                     ESNGETLYLGSKKSQCRFCFYQKDHEQRKRRGIPLEEAKVKNRFELRYRKEKAQSLAK
                     IISKTHDLTKLFFELLNGAICFYDRDPNDLGAKVDAKWAAFISNYGAITISLDTIPQS
                     FEKTMSWLIHGVSPSLVFIAQVDQTFHSSLLPLIIEQGEMNPRQIKLLQNMKADPEYY
                     QEEVEYYERKLQSISNEKTSY"
     gene            complement(4609..4716)
                     /locus_tag="ACHAZB_002311"
     CDS             complement(4609..4716)
                     /locus_tag="ACHAZB_002311"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8614937.1"
                     /translation="MNQVNQHDLGEAIRVSREERGWTQRYLAEKVGISR"
     gene            4879..5691
                     /locus_tag="ACHAZB_002312"
     CDS             4879..5691
                     /locus_tag="ACHAZB_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8614938.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1717 a         1189 c          921 g         1963 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaatcctt ttactagcaa atactagtaa aaggacgatt atactcgtgg taccaccttt
     1321 tttcagaaaa aacattcttt ctctcagcct gacaataaaa tcagtggtct tggtaacgaa
     1381 agactgttcg tttttgccta cctttcgaca aaaaatcctc taaggccatt ttcgacagtc
     1441 aacttctagc tttttacacc aaccaaagcc tctcttgaac cattttctgt ctactttcct
     1501 tctcatcgga tactattaga ataaaattag aatagcatgt ttgaaagaaa agtcaatagg
     1561 gaataagaac aaacaaaaac taatttgtta ttcttagtaa tttgtttgtg gaatacaata
     1621 aagcttttct aatatgataa ttgttcagtc gtcctttcat cttagatcac ttccttaaaa
     1681 tgatcatacc cgtttcaacc acataaaaaa ggacttaatt ttgaaaaatt tcccatcaaa
     1741 actaagtcct ttttattata gttatgcaaa tttgcttatt ttctcgctat ttccgataca
     1801 tcttgaacag gagcaattcg actgaaaaca ctggtgcaaa agcatttatt tgtcttatgg
     1861 tagaaccgtg gaaaactcag actggtaata aattgtcatt ttttgaaaga atagcgccct
     1921 cttgaacatt atcaatcgca cttaccactt gttgatctga ctttgcatat aagtgaccgt
     1981 aagttcctaa agtgatttga atattcgcat ggcccaatcg ttcttgaata gatagcggtt
     2041 gaatattctg ctcaattaga aatgcaacat gagaatgtcg tagcgcgtga agcttaatcg
     2101 gtttgacacc cgctttttta gcagcttctt ttaaccagtt cgcaaaagaa taaggtggcg
     2161 taaatgttcc gtccaactga gcaacatatt caaaatttcc aatacgtttt tgcaagtcat
     2221 accactgtct gagttttaag gaaagcttct tccccaaccc aatcgttcga taagcacatt
     2281 tagttttggg agtcgaaaag taccaatcat cttttgtatt ataaatcaat gtcttctcaa
     2341 tcgttatcgt gtttttctca aaatctacat tcgaccaaga caaagcttca agttctccaa
     2401 tccgaatgcc agtataaaag agcatttcat acacaatctt ccgatggtgg gtttgaatct
     2461 tatcatcctt caattcattc atcacttttt gaaactcact gtgttcccaa aactcaactt
     2521 tcttattctc agaaggaagc gaagaaacat cttcaacggg atttttagca atcaccccct
     2581 ctttaacagc ccgatccaat acaattttta ggtgccctcg aatccgattg atataagatt
     2641 ttgataacgt ttcgccatta acttttttcc gtgaattacg tgactctgtc tggcttagtt
     2701 caaataacca atcctcaata tcgtgacttg taatatctcg aacacgaaaa tgatagaaaa
     2761 actgcatacg cttttcgaaa atgaattgcg cattctcata tgtttgactt ttgacctgtc
     2821 tctcgtacca ttttacaaac ctatccttgt aaaactcttc gaaagtcatt tgattggtag
     2881 aacacagttg attaggaatt gtcttgttaa aatcatgtgt ttccatgtac ttgtcataag
     2941 caattttggc atctttttgt gaagcataac ctcgtttagt cttctgaacc tttcttccat
     3001 tttcatctat acctagatac gctcggaaga accatttttt tgtcttttta tcttggtaaa
     3061 tatttttaat ttttggcatc gttccattcc tccctgtact tgagaaaagc tactttaata
     3121 gtaccgcgct tgtactgttt attccgcaac ttgaatgtga aaaagatgtt caattactcg
     3181 ggatggcacc acaccaactt ggcgattatt ataaaaatca ataccttcaa tccttgcaag
     3241 atatgccttg ctctctttaa tcattcgtgc agcttgacta ggtttaaagc ccatctctat
     3301 caaaattgca cgatttacca tgaaatcaca ttttgtttcc attctctcac ctcctgattc
     3361 tttactcctt ttaaaataat cctctttata atctattgtt tctcattcga tggttgtgtt
     3421 atactcgtct tgtcgaggcg gagtacgttg cacaacttgt tgtgtaagca gtactttgct
     3481 tttttagtag cttgttttct catttgatat actttgtaat tttcgttcat aatactcaac
     3541 ctcctcctga taatactccg ggtctgcctt catattttgg agaagtttga tctgtcttgg
     3601 attcatttct ccttgttcaa taatcaaagg caacaaggaa gaatgaaacg tttgatcgac
     3661 ctgtgcaata aaaactaatg atggtgaaac accatgaatt aaccaactca tagttttctc
     3721 aaaactttga ggaattgtat ctaaagaaat ggtgattgcg ccataattgc taataaaggc
     3781 tgcccatttg gcatcaactt ttgcacctag atcatttgga tcacggtcat aaaaacaaat
     3841 tgctccatta agtaactcaa agaacagttt ggttaaatca tgggtttttg aaataatctt
     3901 tgccaagctt tgcgctttct cttttcgata ccgtagttca aagcgattct ttacttttgc
     3961 ctcttctaaa ggaattcctc gtcgcttccg ttgttcatgg tccttctgat aaaagcaaaa
     4021 acgacactga ctcttctttg agcctaaata caaggtctca ccatttgatt cattgtttat
     4081 gatatccgta ccgccatgag attcactcac ttgaaaagtg gtccacacat ggcctagtat
     4141 taccttatgc ttaagttctg gaatgctgag actccccaca aaatcatcta aagtaaaatc
     4201 aatccgtgta aagttcccct gataatctaa taccttacga aaaaactgtt gccaatttgt
     4261 ttttcgtgtt tttagccatc ttgctaagtg tttacagcct tgacctgaaa actccatcac
     4321 agttccttta accgcatcgt caatggaata ccgaatcgtg attacttgat tccacacgta
     4381 ttggccaata tagccctttg gcgcataatc cagttcctca aaacgttcaa tgggcatttc
     4441 aataatctct tcaattagct ttaaatgttg attcgagaaa aagtgaatgg ttagataatc
     4501 aatcaaaaca cggttaactg gaacgacctc ttcttgcaaa ctatccaaca caagattcaa
     4561 tttttcttca cttaattgtc tcgttccttt ttctactttc gataataact atctcgaaat
     4621 ccccactttt tctgctaaat atcgttgtgt ccaccccctt tcttctcgag aaactcgaat
     4681 ggcttcaccg agatcatgtt gattaacttg gttcatcgac aaacctcctt ctgctattta
     4741 cttgtcaaag aacctattga acttatttta agtacatttt tattgtacgc ctgtatttat
     4801 tttaagtcaa tatgttttga ctttttttaa gtacacacgt atactaaatt taagaactta
     4861 tcctgaggag gagttttcat ggagatagac aagcaagcag tcggcaatcg aattcggcag
     4921 attcgccaag aattaaagtt gagtatggag aaatttggca aactaattgg tgatttaccg
     4981 cgaagtacag tcaataactg ggaacgtggg atcaaccttc caaaaaccga aacccttcac
     5041 caaatcgcag agataggtca tgtcaccaat gaatatcttt tgtatggtga tcaagagaat
     5101 caatacattt tagaaatgct ccaaaaaaag gcaggtaagc ttgatcctaa gattgaaggt
     5161 ctcatagtag acgaaatcaa acacgctgac cttgttagtg aaaaagaaat ggatcgaatg
     5221 attgaatttt ttattacaaa tctcattcca ccaactgaac aagaccagtt tactttccaa
     5281 tgtatcgatg aaaaaaagca gctttatctt ggttcaacta attttggtaa ggaagcccag
     5341 ctctatttgc aacatgataa tcaaaatcat attctgcaca tgatgccctt taccttctcc
     5401 aactttagtg tggatcgcat actcgtctat ttagcaaatc aaaaatccta ttcctatttt
     5461 gggaaacatt tacccaagga actaggtgag aaagccatcc ttctatactc aattaatcaa
     5521 ttgaccagcg atgtgcgaat tgccccgttg gtctattcag aagaaaccgc ttcttatcag
     5581 tacacagagg acaatcaata tctactagaa cagcaatatc tttatctacc attcgtgatg
     5641 gaagtcgaga aagaacgcct gttaaatgca gcgtatccat catcaaaatg aggtgacaaa
     5701 attgataagc acgtgacaaa gaaaatactg ctaggacact ccccaatggg tagttgtcac
     5761 agtttcctgt tctttgttag ccccctgtta
//
LOCUS       DAYYCY010000149         5775 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000149 DAYYCY010000000
VERSION     DAYYCY010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5775
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.150"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            171..1067
                     /locus_tag="ACHAZB_002313"
     CDS             171..1067
                     /locus_tag="ACHAZB_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFM8614939.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(1217..2158)
                     /locus_tag="ACHAZB_002314"
     CDS             complement(1217..2158)
                     /locus_tag="ACHAZB_002314"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFM8614940.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            2284..3405
                     /locus_tag="ACHAZB_002315"
     CDS             2284..3405
                     /locus_tag="ACHAZB_002315"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFM8614941.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3663..5165
                     /gene="eat(A)"
                     /locus_tag="ACHAZB_002316"
     CDS             3663..5165
                     /gene="eat(A)"
                     /locus_tag="ACHAZB_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFM8614942.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            5232..5759
                     /locus_tag="ACHAZB_002317"
     CDS             5232..5759
                     /locus_tag="ACHAZB_002317"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFM8614943.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
BASE COUNT         1895 a         1022 c         1216 g         1642 t
ORIGIN      
        1 caactattca gaaaactttt aggcgaattt taatagaaga atgatttatt tcatcatatt
       61 ttcatacaaa tgttaaattt tcatacaaat ataggaaacg ttttactggc tttttactat
      121 ttaaacaggc tatcatagga aaaagtgaca gagaaagtga ggcggaaaag atgaacaaaa
      181 atgacgtact gctagttgca aaaaataaat cactttattt tttgccttcc atccgctctt
      241 tagatgtttg gtccggaaaa gatttactac agtcggaatt cattaaatca gctagacaac
      301 agttggcaaa ttatcagctg atcgtttttt tagattatgg atttgatatg gagatggctc
      361 gacgcgtgcg cccatttacc aatgcaaaaa ttgtgttatt tttttggaat cacttcaaag
      421 cagaacacga ggcgatgctg aagcaggcac aagatgaacc ggcaatcgat gagatttatc
      481 attttgattt tttggaagca aaagaacttg ggctgaaaca taatagttca ttttattcga
      541 aacatatgaa tttttcttct agcgaaccaa agacggatct attttttggt gcaacgaata
      601 atgggagaaa agaacgagca gaaagctata agcaggaatt tataaaaaga gatgttacga
      661 ccaactattt cattcttcca catcgaggga atgaacaacc tggatatctt acttatgaag
      721 agtacctgaa aaaaacaggg gaatcgcgcg ggattctgga actgttgcgg gagggtcagc
      781 aaggagtgac tttgcggaca tttgaatcca tgtattttca aaagaaacta gtgacggata
      841 atcaagcgat tgctcattat catttttatc gtcctgaaaa tatttttttg cttcgagaac
      901 gtgatttaga tgaattaccg gaatttctgc atacaccatt taagccaatc ctatcagagc
      961 tattggaatt ttatgatgca gaaaattggg caaaacgatt tatcactaac gattccacta
     1021 tttatgagca atacgaatat gacctctcct ctgttgaaaa caaataaaaa acctacattt
     1081 tgaagagatt atgtactgaa acaaacaagc acaattctta tggcatcatg agagttgtgt
     1141 tttctttttg atcttttatt gggcttaaaa aaagagaacc agctgctgat tcagctgatt
     1201 ctcatcagag agccttttat tctgcgccaa ggattccttc ttttgcatgg actaattggg
     1261 tcaaaaaggc acaatatggt gtagcaatat catatttttg tccttttcgc caaatggcac
     1321 cattgatgta gtcgatttca gtcaaacgat ggtttttgat caggtcttgg tacatagatg
     1381 ggtaatgcaa cccgatgttg tcaggatcat aagtagaagc aatatgttgg tacacttctt
     1441 cttgatctag tatgattcct tcttttgctg cgatagaagc aaattcagat acgattgttc
     1501 gaaccatgct ctctgccgct ttttgcgcgc ctagttcagc aatattacaa tccaaaatcg
     1561 tgcataaacc attcaatgtt ccatttacac aagcttttcg ccaaatggaa taacgaacgt
     1621 tatggctata gatcggattt aaatttgctt cttgaaagac ttctacaact ttttttgtaa
     1681 atgcttcgcc ttctggttcc aagttctcta gttcgatttc tccatcacct aacaatgtga
     1741 cttttccggg accagctaga ccagctgtcc acatcgtgat cccaaataag atatttttct
     1801 ttggcacata tttttccagt acatcttcat gacctaatcc atttagtaaa cacaaaacat
     1861 aagtattgtc agtaataatg gattggatcg aacaaaacat atcatctaat tgattggctt
     1921 ttgttaatgc tacgatcaag tccacgtgtt cgttgctttc gatgatttct tctggtgaat
     1981 agattggcag ttttgcgacc acttctttac cgttaaagtc agcaatcaac ccatttgtac
     2041 gaatggcttc aatgtgagca ggccaacgat cgatcaagag gacctcattt ccgctttgat
     2101 gtagcatcaa tccgattcgg ctgcccattg ctccggcccc agctattgcg attttcatgt
     2161 tttttctctc ctttattcaa tgagttccct tttagtatat agaaaaatgg aagaaaatag
     2221 cattgttttg tttgaaaaaa atgaaaaaaa gtgtcttact taagaagaaa gtgagggata
     2281 cagatggata ttttggcggc aattgattca tttaaaggaa gtgcttcttc atttgaacta
     2341 aatcaggcag ttctaaatgg ctgggaagat cgtaaaggaa gaaaaatcaa tgtgccgatt
     2401 gcagatggcg gagaaaatac tgcagaagca atctattacg cgctcagggg aacatggcgt
     2461 accattgatg ggatggatct cttattccgt cagcgaaaaa tcagctattt attgacaact
     2521 tggcaaggaa aaaacaccgc agtcatcgaa tctgcacaag cattggggtt agatctactt
     2581 gataaaccaa cggatcaaac tatccgactt gcttccagtt atgccttagg agaacttatt
     2641 aaagatgcac tgcttcaaga agtagaacaa attattgtca ctttaggagg aagcgggtgt
     2701 agcgatggtg gtctaggatt actccaatcc ttgggtgctt ctttagaagg gtacacagaa
     2761 ggaaatcctt tattatctac taaaaaactt cattttgatc gaagaaacgt tcttgtttct
     2821 aaagtaaaat tgattatcgc tgcagatgtt accaatccat atacaggtca acaaggagct
     2881 gcttggcaat ttggaagaca aaaaggagga atagatacga cgctagcttt cttagataaa
     2941 caagcaggaa agattgctca gcaaataaaa gatttctggg aggtggattt agatgatatt
     3001 ccaggaagtg gagcagcagg cggtattggt ggagcccttt ttttgttggg agcgaagatg
     3061 gtttctggtt tcgatttagt cagccaaatc actggattgg aaaaaaagat cgaacaagca
     3121 gatgtcatca ttacaggaga agggcggatc gatcagcaaa cgatccacgg aaaagttcca
     3181 tacggtgtag cagtgcttgc aaaaaaatat cgaaaaccaa taatcgctat ttgcggaagt
     3241 agagaccaag agctagaagg actttctgat tttctcccaa ttgtcttaag tattcatttg
     3301 ggtccaatag gtttagaaga agcaatggaa cacaggcaaa cattagaaaa aacaaaaatc
     3361 ttaggacaga cacttagcag atttttcacc atcctcccga agtagttgac aaaatcattt
     3421 tctttatgct atcgtaattt caaatatagg acaaagtgag ggatccgtgc attgaaaaat
     3481 taagtcgatt gatgaaagct gcattcagac tgtaccttgt ggagacaagg aagcagtgtg
     3541 cagattttcg ggattggctt ggttttttcg atgtatgggt ctttttgtgt gtatccaacc
     3601 cttgcatcct ctttagccag ttctgaaaat aggactggtc tttttttacg aggtgatgga
     3661 aaatgtctaa aatcgaaata aaaaatctga cattcggcta cgacagccaa ggcacattat
     3721 tatttgaaca agcaaatcta aattttgaca cacaatggaa actaggactt atcggacgaa
     3781 acggtcgagg aaagacaact ttactgaata ttctacaaaa caaactacct tatcaagggc
     3841 aagtaatcca tcagcaagaa tttgcctatt tcccgcaaca gacaaaagat aaagaacgtt
     3901 taacctatta cgtgttaaat gatattacgg attttgagat atgggaaatc gaaagagagc
     3961 tccaattgat gcaaacagat cctgaaatct tatggagaga attcagcaca ctatcggggg
     4021 gagagaagac aaaagtccta ctggcacttt tatttgtgga tgacactcat ttcccgttaa
     4081 tcgatgaacc aacgaatcat ttggatatct ctggtagaaa acaagtagcg gcttatttga
     4141 aaaagaaaaa acaaggcttc atcgtggtca gccatgaccg gggatttatc gatgaagtag
     4201 tggaccatgt tttagcaatc gaaaaaagtc aactggaact ttatcaaggg aatttctcta
     4261 tctatgaaga acagaaaaaa cttcgtgatg aatttgaaat ggctcaaaat gaaaaattga
     4321 aaaaagaagt cagtaggcta aagaaaacag cagctgaaaa agccgaatgg tctcgttccc
     4381 gagaaggaga taaaacaaag aaacaagtcg gattcatcga tactgaatct agacgagtga
     4441 ataaaggagc agtgggtgct gatgctgcac ggacgatgaa acgatccaaa gcaatcgtga
     4501 atcggatgga gacccagatc agcgagaaag aaaaactatt aaaagatatc gaatatatcg
     4561 attctttgac gatgaatagc caagcgtctc accataagcg acttttaagc gtagaagatc
     4621 ttcaattagg gtatgaaaat ctgttattcg agccaattca ttttacaatc gagcctcatc
     4681 agcgggtggc gatttcaggt cctaacggtg caggaaagtc atccattatc cattatcttc
     4741 tgggggcatt caacggcaag gttataggag aaaaatacca gccaaaacat ctgagcatta
     4801 gttatgcaag ccaaaattat gaagacaatc gaggaacgtt ggcggaattt gcagagaaaa
     4861 accaagtaga ctaccaagca tttttgaaca acctccgaaa gcttgggatg gaaagagatg
     4921 tttttcataa caagatcgag cagatgagta tgggccaacg gaaaaaagtg gaattggcta
     4981 aatctttatc acagccagct gaactatata catgggatga accattgaat tatttggatg
     5041 tcttcaatca agaacaatta gaacaactga tcttgaacgt gaaacctgcc atgttactag
     5101 tggaacatga tcaaaccttc ctggataaag tatctactga gattatttct cttgagagaa
     5161 tctaaccctt gtaatctcaa gaacaacaag gtatggttaa ggggaacttt gccaacgaag
     5221 gagagaatca catgctttat gtaacgagac acggagaaac aacttggaac gcacaaggat
     5281 tagtttgcgg acgggcagat gtgccgctga ctgaaaaagg acagatgcag gcacagaaac
     5341 tagcagaaaa ggtagtagat ttgcctgttc caatcacgaa aatcatccat tcccctcttc
     5401 aacgagcaag agatacagca caagcagttg ctgatcgtct cagcttgccg ctgaccgttg
     5461 atgaacgatt ggtagaaatg gattttgggg attatgatgg actgccaagc aaagatgaga
     5521 actttcaaaa agcacgttta gcgttcgctg tacgattccc aaatggggag tcagtcttag
     5581 atgtgtatgc acggatcgtt cctttattga aagaatgcat cgaagacgaa gaaaatgtct
     5641 atctgttagt ttgccataat gccttgataa gagtgatcaa tgcttatttt catccgatgc
     5701 ctaatgaggg gttctttaca ttcatggtag ataatacaga attggtttct tatgagtaat
     5761 tatgtttttg tgtta
//
LOCUS       DAYYCY010000150         5766 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000150 DAYYCY010000000
VERSION     DAYYCY010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5766
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.151"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(256..543)
                     /locus_tag="ACHAZB_002318"
     CDS             complement(256..543)
                     /locus_tag="ACHAZB_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFM8614944.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(563..787)
                     /locus_tag="ACHAZB_002319"
     CDS             complement(563..787)
                     /locus_tag="ACHAZB_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFM8614945.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(1242..1412)
                     /locus_tag="ACHAZB_002320"
     CDS             complement(1242..1412)
                     /locus_tag="ACHAZB_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8614946.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(1427..1960)
                     /locus_tag="ACHAZB_002321"
     CDS             complement(1427..1960)
                     /locus_tag="ACHAZB_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFM8614947.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(1981..2925)
                     /locus_tag="ACHAZB_002322"
     CDS             complement(1981..2925)
                     /locus_tag="ACHAZB_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFM8614948.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     FTFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKIN
                     GDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFA
                     YFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELL
                     QQIKQLEKEKKALEKQTESALSEKEKKSLLSDLDNLFQ"
     gene            3562..3648
                     /locus_tag="ACHAZB_002323"
     CDS             3562..3648
                     /locus_tag="ACHAZB_002323"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFM8614949.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(3769..4458)
                     /locus_tag="ACHAZB_002324"
     CDS             complement(3769..4458)
                     /locus_tag="ACHAZB_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8614950.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(4465..4743)
                     /locus_tag="ACHAZB_002325"
     CDS             complement(4465..4743)
                     /locus_tag="ACHAZB_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8614951.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(4703..5617)
                     /locus_tag="ACHAZB_002326"
     CDS             complement(4703..5617)
                     /locus_tag="ACHAZB_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFM8614952.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(5599..>5766)
                     /gene="mobC"
                     /locus_tag="ACHAZB_002327"
     CDS             complement(5599..>5766)
                     /gene="mobC"
                     /locus_tag="ACHAZB_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HFM8614953.1"
                     /translation="QAIARDLAKAGGNINQIAKWCNTHQHDVDPGDAQRLSENLKIMQ
                     KELQKIWQQLK"
BASE COUNT         1530 a         1049 c          960 g         2227 t
ORIGIN      
        1 cccccccact ctccacaata caaaaaagac tagcaaatgc tagccacttt ttgtaaagtt
       61 tcgcttgttt caccttcggt gaaattgttg caaatcaata attaggtaga gctacatttt
      121 tttcgttgtt caaagattct tatgccattt actattaata aatctataaa tatgataatt
      181 tttcggctct ataatttatg ggactgatga atcagaattg attcttcagt cccattttca
      241 ttttattcca aagaattaac tccaaatacc aatagaagcc catcctctat atacttgaga
      301 ttccttacta acaatttctt gtaattctat taaagattta ggtaatccaa atttatgttt
      361 taaaatatat cttccaagaa aactacttaa tttcatacta acttcaccaa cactttttcc
      421 ctcactgatt tctttagaat aaagaaagag cttttctttt aagtcaggat agtcatctat
      481 agttgcttca ttgtatactt tactaattgc atttaaaagt ttttcttctt tagtaaaatc
      541 cattataata acctccctac cactaccttc taggtgccca tggaccatga ttaacccaac
      601 cattaacaat aatttttcca atttctcctt tagcttgatt ccaatctacc caacattttt
      661 ctttgttaca ataaagacca tttccatagt aagtagctgc atcaactttt attcctgtac
      721 cgatgccagc tagacaagtt cctaatattc ctagaataac acaatgtttt aatactttct
      781 ttttcatgat tgtttttcct tttcgttttt ttaaaattgc ttgttatatt aaataatctt
      841 agtaatttta ttatataaca ttttattata taacaatgat taacgtcata aaaagtagtt
      901 ttgaattcat aatcgtttaa ataaaattat aagatcagtt atttaaacag ctagtagaca
      961 taatgtgtat actaccaagt aacgcctgta ccaagggatc caggcgctga ttttgtgaat
     1021 ttttattggt ctaggttaaa agctcttttt aaggcttggt tttctgcggt tgtttctttc
     1081 taattctttg tgaatattga tcggtcattt tttaaatact tgttattttt gtttgtgttt
     1141 gttatgcttt ttacatatct catattcatt tattattctt ttctaaggtt aataatagga
     1201 taatctcttt cagaaagact gctcgtacga gcagtctttt cttatttatc aagtttttct
     1261 acaaatactt cctcattttg actgttgtag taattaggat tgagttttag ttcccctttt
     1321 ttatttcctt tgttggccac tgataataac attgggctaa agcttaatat tcctagtagg
     1381 atagaaccga atagcaagtg tttttttgtc ataagtattt ccttcttcat ttccatagct
     1441 gccaccattt tttccaattc tttgtttgtt cgtccattgt ttctgttgct tcagaaagag
     1501 tcgttgaact attctctgaa gactcttccg tataagtcaa ctgcagttgc tcctgaagtt
     1561 tttcgatttg ttggttggct tgcagggtga gttgttgttg ctggtcgagt agtttttgta
     1621 tatgagaatt attttgctct ttgagggcaa gttgttctcg taaaaattca ttttcttcac
     1681 gcaactgatc tagcaacttg tttgcaacca cgtttttgtt tggtatataa gcatttttat
     1741 cgttgttttg ttcatgttta tgcaacttat ctagcaacct ttgattgtct aagttgcgag
     1801 caagttgcaa tactccagaa cgcaatacaa ccttataacc acgctcattt ttagctatca
     1861 tggaagaatc taatttttgg atatgtttcg atattgcttg cctactcatc ttaaaaagat
     1921 ctgacaattc ctttattgta aaggtttctt gtggttgcat actggttgcc tcctaggttg
     1981 ctactggaat aaattatcta aatcagataa taaacttttt ttctcttttt ccgaaagtgc
     2041 ggactctgtt tgtttttcaa gagctttttt ctctttttct agttgtttga tttgttggag
     2101 gagttcaagt tctcgtttct tctgtttgat tttttcgtta attgttgtag cgggttcttc
     2161 gtattctttt tgtttatcag ctaaagccaa tgctttattt tggtcttctt tggttggtgc
     2221 atgtttgtta aagtcgtctg cttttttgat ctcaggtttg aacgtaaatt gatagccaat
     2281 aacaggtttt ccccgtcctt ttccgtattt ctttttgatt gttagtcctt tgaagattgc
     2341 agttagttct tgacgtatag gttttataac tcgtttatct atatcaggtt gcttataact
     2401 tttaggaata tctaataatt caagaaagtc ttctttggag aaatatgcga aacctttggt
     2461 tctgtattgc tttaacagac gaaacatagt ctttgcatag ccactttgta gctcgttgaa
     2521 ttgttgtaaa gaatagcgta cccactcgtc taaattattt aacaaaggca gtgctttttc
     2581 gtaaattcgt atatctacgt aaggattttc tacgtccccg ttgattttaa attctgtaaa
     2641 cattacaaac atatcgtaag aaagcccaga ctcacttcta cgaccaaagc gtaaagataa
     2701 gattttttca taagttgctt ttaaatcttc tacaaatcgt acatttgcag ttgctttgta
     2761 gttacttaaa tcttttagtt ggtcaaacgt aaaacgaact gtcttatccc ctttttcacg
     2821 cattctagac acgatggaaa aaaacaaatt catttcaacc ggtgtaaatt ttcttaaagg
     2881 aattgtattt aattcatggt ggtattttac aatctcatta gccatataat cacctcaatc
     2941 caattatgaa tgacattttt taaatgtcaa gacattcata tttgacgttc aaacagacaa
     3001 aatgtccttc ataaacagac aaaatgtcct tcataaacag acaaaatgtc cttcataaac
     3061 agacaaaatg tccttcataa cattctgtat ccattggtag agtaaggaca cagcttacgg
     3121 taaagaggtt taaaaagagg tttaaaaaga gatataaaaa gaggggggct aattttcccg
     3181 cctaaaaacc acttcttttt tctttaaaca ggttcgcgca tttttcaaaa ctgccaatcc
     3241 aaaaccttgc ttcctacggt cgccaaaaag gtgtgatcgc tacgctctca cattgtactg
     3301 cgcagtcgcc taaaggctcc tttgctaaaa acatgttggt aaagaggcct ttgagcggtt
     3361 ctgaatgctt atctaagccg ttttaagaca gtcttggata aaaatcctta tctagcttta
     3421 aagcgcctta caggcggtca gggcaagccc taacaaccct aaaataagcg atacttaaaa
     3481 acaccacaca agtttaattt tttaagatat aataacttgt cgcctatccg acagtcttgc
     3541 gactggaagg aggtgtatct aatgtttgaa ttgttcattt gcccccttct tatagaagtt
     3601 tgtgtctctt tattcagtta ttggttgaat aaccgaaaca agaagtaaaa tcaataaagg
     3661 cgacatcaac ctgaaaaaaa gccccagaga atccgacctc tctggggctt tcgtgtatct
     3721 aatgtttgat catttgcatg tttagtttaa catgattgcc gatcaaattc aattttgctg
     3781 ttgctgatct aaccattcct tgacactttt tagataattt aatgccgttt cttttttcgt
     3841 tggattttgt atgttcgttg cttgctgaag cagtgcttta taacgttcgt aggtaactga
     3901 ttgttgccgt tcttttcgtt cctgttcact agttgaaaaa gggattgtat cactgaataa
     3961 attataacaa gcttcttctg tctgcgtatc tttaaaatat tgcacgtctt ttttggttcg
     4021 ctcatagttg tcttgaacct ctttttgctg tttttctggc aagttttgat aaagacgagt
     4081 gagtttttcc atgtttttat ccgtcaaacg tttttcttga ttcacaaaaa tatcgttcat
     4141 ttcctcataa caagtggtaa actcttccat tttttgtgtt aacacttgct cctttttctt
     4201 tttatcacat gctgcttcat acttttgagt gatatcccct aataaaatac ttcttgaaat
     4261 acgtttattt ccctctaaaa cgccgaataa accacttaaa acgattgata atgtaaatat
     4321 aacccctggc aacaaaaacc tcttaaatcg aattttagag ccttttaatg aatatcttgt
     4381 ttgataagcc ttatagctaa gccaaataat tcctaaaata ctggcaaaaa tgagtagata
     4441 gggtaaatag aatgccattt ctatttattc tccccatacc agccaaaagg ctaacagtgt
     4501 catgattaag caacataagg gggcaagaat atccattgat cgctgcataa ttactttttt
     4561 ctgttcttta tttcgctggt ttaatagcgc atggtggaat tttttgactt gtgaatcaat
     4621 tgtagtttgt attctttctt tggtataagg tgcaagaaaa gcacgttgtt tctttatttc
     4681 attttctatg tcttgtaacg ttttagaacg ataattcttg ttggtagtct gctcctcttt
     4741 caacgtgttg gtcatggttt cttcgagtcg gtctaatttc cggttgttca gtcgtaagag
     4801 ttcgttgttc tcttcgtact tcttttgcag tttcgcgaat gctagctcga gctcttcgta
     4861 gctcgttggt tcgttgttga ttgtaggctc gttctcgtct gtccagctca tgtaaaatcg
     4921 tctccttttc gtaatccttt cctagtttgt tggatcgtac ttttttcttt ttgtggatca
     4981 attcgtaagt aaaatttttc ccacgattca agcaatgaat gccgaatgtt ttaagagaat
     5041 cacaaaattg gttatacgtt cgagtttttt tgtttttcat tgtttgatca atcgcaaacc
     5101 gaatttcgtc tttccaaaca ggttcatttt tttcttttaa tttgatttca gacatggttc
     5161 gtttttcggg tttcttccct cgttcagtga ttgttaagtc atgttcaaga caaatctcgt
     5221 catttaggtc ataacatttg tttaaaaatg cttgaccatg ggcttgaaat ttgttcccag
     5281 tttccagatt gacggagtta atgacaatgt ggttatgaat atggtctttg tctgtatgtg
     5341 tgtaaatcgc gatttggtgg tcaggggcta tttttttcgc atactctgcc cctagttgat
     5401 tggctttttc agcggtaatt tctcctggtc gaaaagactg gatcaataag tgtgcttgta
     5461 cacgatcgtc ttttccatac agcatgcgaa tttgtttcat ttcgcttttg gcataatcaa
     5521 tgtcacagtt tatccctgtt ttgacggttg ctcgtggttc ggcgtaattg atcgctcggc
     5581 tacaagaagt ggttcggctt actttaactg ttgccatatc ttttgtagct ccttttgcat
     5641 gatttttagg ttttcggata agcgttgggc gtcgccagga tcaacgtcgt gttggtgtgt
     5701 gttgcaccac ttggcaattt gattgatatt cccgccagct ttggctaaat cgcgcgcgat
     5761 tgcctg
//
LOCUS       DAYYCY010000151         5562 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000151 DAYYCY010000000
VERSION     DAYYCY010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5562
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.152"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            200..1075
                     /locus_tag="ACHAZB_002328"
     CDS             200..1075
                     /locus_tag="ACHAZB_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFM8614954.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(1220..1372)
                     /locus_tag="ACHAZB_002329"
                     /pseudo
     CDS             complement(1220..1372)
                     /locus_tag="ACHAZB_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(1640..2971)
                     /locus_tag="ACHAZB_002330"
     CDS             complement(1640..2971)
                     /locus_tag="ACHAZB_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM8614955.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
     gene            complement(3120..3833)
                     /locus_tag="ACHAZB_002331"
     CDS             complement(3120..3833)
                     /locus_tag="ACHAZB_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8614956.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            4008..5432
                     /locus_tag="ACHAZB_002332"
     CDS             4008..5432
                     /locus_tag="ACHAZB_002332"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8614957.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
BASE COUNT         1923 a         1000 c          885 g         1754 t
ORIGIN      
        1 aaataaaaaa caaaaaaagt tccatcttta aaacacgcta ttcttttact ttttctcaca
       61 ttctctaaga aaagaaaagg agaatgaatt tttgacaatt cgagcaaaag tgattatcaa
      121 atatgtaaaa tcagctactt ttttcttaaa cgtttcttta tactaaatat aaataagtgt
      181 ttcatgaaag gagaactaga tgaataattg gcttgagtat tttcctgaaa acgttttgga
      241 acgcggttac agctatcatc tgcacggttt tgtccgtcat ttgaactata caagcaagta
      301 cttgtccgca acagtttctg gcacggaaga ttataaggta gtgatcactt gggatgaaaa
      361 gaccaacatg acttgtgatt gtctatatgc catagaagga aaaaaatgca aacacatggc
      421 tgctgtctta ttcgcttacg aagaacgtcc aataaaaaaa tccaactact cactttccga
      481 actctcgtcc cttgtttcct ccgctagttc ctctcttgta cgggagcttt taacagagat
      541 tcttatagaa catccgcaat ttatcgaacg cttcaaagtt aagatgccat ttcatgcaat
      601 aaattattca gataaattaa caactatcat ccataaatac gatcatatta tcaagaaaaa
      661 caaaaacagg aaaacagcga agttcatcat ggagatgcga aagttcatcc aagaagcagt
      721 tgaatcgctc attcagcaaa atgcctatct tcccgcattc gaattgatca atgaagtgat
      781 tgctactctc gaaacattct attgggagcc tgaggatgaa cgaacccttc ttctgataga
      841 ggactgttat tatttatgga aagaattatt agcagaagca cctcatgcag aaaaaaggca
      901 aatgttctct tggttcgtct gccaagtcga tcatactgac gcctcttatt caaaacgcta
      961 ctccataaaa atcctgaaag aagactttcg ggaaaaagaa ttttctaacc aaaagaaaaa
     1021 aatagataaa aaaactaaag aaacggtaaa gaaagacgat ttcaatgaaa aatagcaagt
     1081 aaacgataac aaccatccta aatccacaag aaatacagca gaccttgctt taaaaacacg
     1141 aatcatgcaa aggatgaagt aaagaagact taacgcgtac ccaatattag tcatttgact
     1201 attaggtacg cgttttgttt tataagtcat gacaaatttc cttcgttgct gggtagattt
     1261 tccggcattg ttgatagatt ttatgatact tttctacatt ttgtaagaga ggttccactg
     1321 catctgtata ttggataaaa ttctgacaac aactttcagg tgtgtcgaac cactctaatc
     1381 tttaaagata aatattaaaa taacgattaa taatattaac agtctttatt tttcaccacc
     1441 aaaaacctat ctttgcatat actaaataga cgtatataca cagataggtt tgtcttgtga
     1501 aaaataatga ttatccttac caaaaaatgt ttgtttttct acatgttcgt acttatcaaa
     1561 aaatatgatc cttattgaaa ttactggcta tttatattcc aacgacttct tgtgttaatt
     1621 tttgttatct catagtaaat tatgtttcat tttcacttaa tttagttgtc acgtgttcgt
     1681 caattcgtaa aaacggtaaa tatataaaca cacttgcggc tataattaat atttgtaaaa
     1741 cagctgctcg ccagtctccg gctgttgcta aaaatcccga tataactggt ggcgtagtcc
     1801 aaggaatttg cactactaca tgattaatta atcccacagc agttgcaaaa tatgctactg
     1861 tcaaagaaaa aatcggtgca agcacaaagg gaataatcag actgatatta aatacaatag
     1921 gcaatccaaa aataataggt tcgcttatat tgaatatagc gggagttacc gataatttag
     1981 caaagtcctt ataatctttt ctacgactga agataaatat tgcaatcaat aaagcaatcg
     2041 tatttcccga tccacccatt accgcaaagg tatctttaaa ggccatattg ataatatgtg
     2101 gaatttgctc tttgtttgca taagctgcca tattttcttg catattctga atcaaaaacg
     2161 gatctaacaa tgaacctgat attactgctt gatggatacc aaatccaaaa aatagattag
     2221 caatagatgt aattaacaaa aatcctggta aaccagttgt aattttactt aatggctctg
     2281 taactatcgt ggtaattagc ttatttatat ccatattaaa caattgaatg attataaatg
     2341 acaataaagc aaacgaggta atcgtaataa tgattgggat taatacatta aaagacttta
     2401 aaacagcggg aggaatattt ccggatagct gaatctgtag tttcttatta ttagaaagtt
     2461 taataaataa ttctgtagcc gctaatccaa caaatatacc tacaaacatt cctgaagctc
     2521 ctgtatagga tataggtatc acacccgtca cttcaacagc tttatccgca ttttcaggag
     2581 taaacatcat ctgcataggt gttaatacaa caaatgtcga aatagcaact aataccgctg
     2641 caatatgatt tgtataattt ctgcttgcac acatatgata agatactgct gctacaacta
     2701 ataatgtagt tatgcttaat gtaccattgg caattgaagt accaatttgt tgtaacgtgg
     2761 tcaatgtact agtatcgaca atttccccca tgaatcctgt tggttctaag atcactgaat
     2821 taataagtgt cacaaatcct gctaacatca taaatggcat aaatgtagca aacgcatctc
     2881 gcaaagttct taaatggatc tgattcccca acttagtcgc aactatacca aatttttcaa
     2941 atacaatgtc cttcctgcta cttttgccca tattttttct cctttatgct tattgtaatc
     3001 gattactttt tccacccaaa taatacatca cttatcaaaa aaagtaaata cttttaatta
     3061 aatatatata tcttttttag tataaaaaaa taagctctga aaaaataatt ttcccgagct
     3121 tatttttcaa caaaaacagc ttcaaaaaca aagtcttgat ataaatgtct cgataccgaa
     3181 tactcgaaag gtttaccaga cgatagagca acaactttgt ctatctcaat cataaattca
     3241 cctggatcta tttttaatat ttttgcgtca aattcattcg cagtatctcc tttgatacgt
     3301 atgtgcgaag tctttatttc cagacccagt tcatttgtga tataagcata tacagatttc
     3361 tttaaatgtt ttggttccaa gcctggtatg actgctaatg gcatgaatgt ttgctcaatc
     3421 gagtatggtt gacaatcaat aaaacggctt cgtacaatat tatatacata gtcttttaca
     3481 ctgatactta atttatcagc tacttttttc gatggaagtt caattgagaa agtatggatt
     3541 tctgaatcta cgtgttcttc accaaccacg ctaaacaaac cttcgtttgg accaagaact
     3601 cgtgagttat caattggtgt tctgacgact tcagtcccat acccacttct acttcgcaaa
     3661 actccttcgg aaacaagtaa gtctacgcct ttctttactg ttaacttgct tgtatgataa
     3721 ataactgcca attcatctcc tgtaggaagt ttctcaccaa ttttatactc tccatttaca
     3781 atttttttct ttatatctaa gtaaatttcc atatacttcg caattttttt caatggtgac
     3841 cctccaatca aaaacatatc ttcatctata ctttaacata aataaaccga taaaatccac
     3901 atcaaaatta tacacacaat aaaaaagata tatatattta aacaaaacta tttactttaa
     3961 gttgtaagcg gtgttatcat taagtggaaa caggaggtaa aaaaattatg acaactatga
     4021 aaaagctaac ttttccagat gatttttggt ggggttccgc ttggtcagct gagcaagcag
     4081 aaggaaaagg agaaacagga aaagcagaga ctatctggga tcgctggttt aaagaacaac
     4141 cttaccggtt ttatcaacga atcgattcct ctattacaac cgatcacatc catcaatata
     4201 aagaagatat agaactaatg aaaaaaactg ggcataactc ctttcgcgtt tctatttctt
     4261 gggcacgaat gtttccaaat ggcggtaccg gaaaagtaaa tcccaaagcc atttctttct
     4321 atcgaaaatt attttccgaa atgaataata acggaatcaa agtatttgcc aacttatatc
     4381 attttgacat gcctgctact cttcaagata aaggaggatg ggaatcacgt gaagtagttg
     4441 atgcttatgt tcattacgct gaaacttgtt ttaaagaatt tggtgactta gtttgtcatt
     4501 ggtttacctt caatgaacca cttgggccta ttttaggtac ttatcttgaa gattttcatt
     4561 atccaaacct cattgacttt aaacgcggag cgcaagcagc attcttcact atacttgcac
     4621 acgcaaaagc aatacaagta tttaaagaac tagctcttcc tagtaaaata ggcgttattc
     4681 taaatttgag tcctactttt cctcgtagtc aaaatcctgc tgatgtgaaa gcagcagaaa
     4741 tcgctgattt attttatacg cggagctttt tagatcctat ggttaaaggg aatttttcta
     4801 aaaagcttat cctgttatta caagaatata atcagcttcc tgaagattat acgaaagaag
     4861 atcttgtatg tatctacgaa aataccgctc aaattcttgg actaaactat tacgagccac
     4921 gtcgcgtcaa atctcgttct tctatggtgg atacatctag tccattttta ccagagtggt
     4981 ttttcgaacc acatatcatg cctggacgac gaatgaacga ataccgggga tgggaaattt
     5041 acgaaaaagg aatttatgat ttgtgcatgg atatcaagaa ccattatgga aacatcgaag
     5101 catttatctc ggagaacgga atgggcgttg cggatgaaga acgtttcatg gatgatacag
     5161 ggcaaataat tgatgattat cggattgagt atatcaaaga tcatcttgct tacttatgga
     5221 aggcaataaa tgaaggatgt aacattaagg ggtatcatct ttggacattt attgattgct
     5281 ggtcatggat caacgcctat aaaaaccgat atgggctggt ttcactcgac cttactactc
     5341 aaaaaagaac aattaaaaaa agtggcgaat tttacaagca attaagtgat acaaatggat
     5401 ttagatacga tgtagacttg ttagttgaat aatattctaa aattccactt gaagtgaaca
     5461 cattagaaaa caagaataat gagaaatacc aataacaaat actttttctt catacttcat
     5521 ttatacttgt cagtataaga gactgtgaca aatgtagtga tc
//
LOCUS       DAYYCY010000152         5559 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000152 DAYYCY010000000
VERSION     DAYYCY010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5559
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.153"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(60..2009)
                     /locus_tag="ACHAZB_002333"
     CDS             complement(60..2009)
                     /locus_tag="ACHAZB_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8614958.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKAEDKDNEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQM
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFR
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPRKERVYGQNFYADDLSMHLV
                     FDMASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSEAGAFEGKGDS
                     NAEIIAKPSVAESKENKIVFSIGGA"
     gene            complement(2051..3109)
                     /locus_tag="ACHAZB_002334"
     CDS             complement(2051..3109)
                     /locus_tag="ACHAZB_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HFM8614959.1"
                     /translation="MEKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLEERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVNKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            complement(3228..3683)
                     /locus_tag="ACHAZB_002335"
     CDS             complement(3228..3683)
                     /locus_tag="ACHAZB_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8614960.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            complement(4267..5070)
                     /locus_tag="ACHAZB_002336"
     CDS             complement(4267..5070)
                     /locus_tag="ACHAZB_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFM8614961.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKALSDKLGTLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
BASE COUNT         1631 a         1101 c          768 g         2059 t
ORIGIN      
        1 tcttacgacc catcgtttta ttctaaggtt ttgtagtaaa tatttcttga atttcatagc
       61 tacgcccctc caatcgaaaa tacaatcttg ttttctttac tttcagcaac ggaaggcttt
      121 gcaataattt cagcattaga atcacctttt ccttcaaacg ctccggcttc actcaactta
      181 acatcgaata tttttgcgtt tggataagta attattgctt cagtaatcgc ttcttcaaac
      241 tcagattcaa ctaaatgcaa gatattcctt gcaccccctg atttagaatc ctcttgtgaa
      301 gccatgtcaa aaactaagtg catggagaga tcgtctgcat aaaaattttg accatatact
      361 ctttctttcc taggtggaag acgtaaatct atatttaaat gttttcgtac taattcagca
      421 ctcttcaaca tttcattata agcaatttcc agaagagttt gttcggataa agcccaaaat
      481 ggaactattc gtccaaatcg gttaataact tcaggcttta gaccttcacc aatcaaggct
      541 tgtttgatcc ctgatgcgac ttttcggtat tctcttttta gttcagtgct gtattcatta
      601 gaattttcct tgtttgtaac acgagatagt gagctatatc ggtttaacga atcgaacatc
      661 tccgcacctg catttgttgt cgcaaataaa attgtatttg acaatgaaac ggtgtcgtga
      721 acttctcgat catcccatcc tctttcagta aaacttatcc taccttcatc taaaattgat
      781 aaaaaggcat cgagtgtttc tacaccagct ttctcatatt catcacacac aataatcgca
      841 gaaggatcat gccttacatg agtacctata agtcgtctaa aacgcattgc atcatctttg
      901 gttttgtagt ccggcatatt aactaagaga actttatttt catcgccata aagtcctctt
      961 gccacggctt tagccattgc agttttaccg acccctgttg atcctgtaaa cagagcagtt
     1021 gcttttggtt gctttcgacc tttaaaacga tcaaatccaa gcttcatacg tttaaacaac
     1081 attgttatag tataaactgc taacggttgt ccttttatgg actttaattc ctcttgaagt
     1141 ttttccatat caaaatcaga atctagctga atattatact taccaaatat ttgcaataga
     1201 ctttctcgat tataagcatt tccgtctact cgatcttttg caatcaaatc ctccattaca
     1261 gcaattgatt tagccggttc ggacaaatcc tcacgataaa gtttgtttgc ttgtacgatt
     1321 tcttgaagta attcatcagg aatcctttgc gataatctaa aagaagcctg aggatcatct
     1381 ttaacagagg atgtatttag ccattgtctc aaaatatcga cgacaacatc gctttttagt
     1441 tcattcaatt ctaacggttg tagccgtctc cctaaggcat catctctttt gatatagttt
     1501 tcatattctt ttcttgtagt agcggtaatc atttgaagtg attcatttgc taacggttct
     1561 tttagcgaat cacccccaat tttattgcca ctcccaaaag agctaacaac tttatgaaac
     1621 tcgtcaatga agaggacaaa ttcattatct ttgtcctcag cttttaaaat tcgctcatat
     1681 tctttcaatc ttggaattaa tgtttctaaa cgtgtttgga ggacatcatc tcccccaaca
     1741 cgcaattgtc cgattgataa tttatatact tgtactttct ttccatcttg tcgctgtctt
     1801 tcagcccatt cacttgcaag tgctgtttta cctgttccgg cttcacctaa caagatagct
     1861 tgcggtgttt ccggtctatt caagatcagt gacaactcat ggagttcgtc atcacgtccc
     1921 actaacttat gttttggtgg tttcattggt gtgagagcat gattaaattc ggtttgaata
     1981 ttcttaaaaa actcggtact attttccatc tgtttgtgtt cacttcctct aaaataagaa
     2041 taatttactt ctagtcagtc aaagttaggt acttaaatgt cgtcttcatg tctccaaaag
     2101 aatctttgtt aaagtcagca tttttgtatt gatcaatcaa tttttgtgta tcattgattg
     2161 ctttaatttc ggtctccatt cgactgtcta gttgacgaat actttcttgc gaatctttat
     2221 cgtcaggatt ttgttgtaaa cgttccagat actcagcttt tgtggcttca attttcttga
     2281 tgttagtatt tgaagcattt aactgatctt ctagcttatt tacaacatta ttcttacttg
     2341 taactgctac aacttttgaa taatcaatat cgccatcttt ggtaagaaag cttccttgat
     2401 agacattagt tgaatctgaa tatggattta ttctgaaatt gatattatca ggagtattaa
     2461 caggttttgc ttttttccca aaagaaatta ctccttcatt attaacatca ttcatagaat
     2521 attgagaaag tgcttttgta actgaatctg cttcattcac attgactttg gtatcatcgt
     2581 cagctgtatc atcaccagaa attagtgata cttggtttgc aataaaaact tgataagctc
     2641 tttcctctaa tttacctttt aggaataaga aagcatcccc atcagtactt aacatcccat
     2701 agctcatttc aagcttctta ggacgatctt tttcttttgt tgccatatat aagttatagt
     2761 tttttccttg cgtagataac aactttctag cgttttggga gtaaccaagt tttactactg
     2821 ttacatctct attttgatct gtccacacat ctgaaatagt gatttctgct cctgatcgag
     2881 caaactccaa tgaagaccct ataggtgttg tattgttcac gagataataa ttatagtttc
     2941 ctaattgata aatgtagcta cccaagcctg ttaataaaaa aagcactcct agaaaagttg
     3001 caagcataat gcgtaatccg tgacgtgttc tcttatcctt atcaccaagc aaccctccaa
     3061 taatactgta ttgatctttg gtagatgttg aattgacgtt tttttccatc agtttgtctg
     3121 ccccctttca taaaagagca aacaatcaga cgttttttcg gtaattctag gttcgggatt
     3181 ttgaatcatt tcggtatatt cttccccatt aatatttaac acaaaaatta tcttcctttc
     3241 ttcgttcctt tcttttgctc tttattttcc aaataatcaa tgatttttgc tattgtacca
     3301 agatagtaat tcccctcagc tttgtttttt cggattttgc taaccattgg agtaggtaca
     3361 atctcttcat ctctcaattc actgaaagat tttttaccta gatattttcc aataacagat
     3421 tttaaaaaac gttcaaaatc tttctcgtaa gttgttttct tgccttttag ttcatggttc
     3481 atattgtcaa cgacttttgc ggtttcaata attgttttag ttctcagctt aataatttca
     3541 tctggaattt tcaatcctcg taatttcatc gttgtactaa ctggtaaccc atgctttact
     3601 gcatcagcaa tactaaaact ttttagatca tctagtaagc tctccatata gttaacaaat
     3661 tcatgccaat taatagcatt cattgtctca ccccttcatt tttttaaaat gaggttcggg
     3721 cgtaacacat ttatgttatt ataatatcat aataacataa ataaatcaat cagcaaattt
     3781 agatatccct taaagtatca gcaattattc aacaagatat ttattttagc tatttcagtc
     3841 atcataaaag actaattagc aataaaataa ctaatattat ccatttatca gacgaaaaga
     3901 aaaaacttat ctaattactc tatattaact tttaacaaat agttgacttg tccaaaaatg
     3961 ttaccttcaa tctcacaaag atattaagat gtttcctacc aaaactgata tcaccggact
     4021 ttttattctg tgttgtccaa aagtgttacc tttagtcgat caacaaatta aaaaaaattg
     4081 tttttttctt gacttgtcca aaagtgttac ccatagttgt taaaatttgt atagattagc
     4141 cattctttcg aatattattt agactgaaac ttgcttacaa aaaaacttct tgcatttatt
     4201 gtccaaaagt gttacccata cttttttaaa gaagcaggtt gtattaaaat tacaacctgc
     4261 ttcttctcaa tcttcttcta accaattatg cattgaaatg gttggcaagt ttgattctgg
     4321 ctcactaaaa gtgaatctga gccgatcaac tcttcttcct ttctttgcat agattttttc
     4381 ataatcaaaa gtttcaaata cctgtattcc gttttcatca ggagctaaaa attcttcttt
     4441 cgcctgctct agtattcttt tgtcaatatt tgtcattcta taacttgaag gtatacttaa
     4501 tgctcttcta aaatcttcaa ctttgactac ccaataacca tttcgccact ctttatttct
     4561 gtattgcatg agatgacgat acaattcttt tgtgtagcta cttcttagtt ttgtaaaatt
     4621 atcaagttca aaacgagtaa agtttttgcc aattgagttt aagataaatt caaattcctc
     4681 atttgcctta attgtaactg tttccttctg tttatctatc cggaattttt gaaaaagcca
     4741 cagctgttca tagacgtctg aatcttcaaa acggtaggtt aaagtaccta gtttgtcact
     4801 caaagctttt aacacagaat aaaaactgtc cttttctctt ctgtcgtatt tgaccaaata
     4861 acgaaagagg ttgaaatcaa aagttacttc tgctgttcct tgacgtttca tttttgataa
     4921 aatagcccaa aacacatcca tttcaacagg cttaaatcct cgcaaagctg ttgtattcag
     4981 accattgtta taacgcacaa ttgcatttgt ttgttctgat actaaatcta catcactatc
     5041 aataatcttt tcggtaactt cttgagacat tgattactct ccaatcttct ttttgcgctc
     5101 ctttaatcat gttaatctgg tactaattaa aggaaacaat tttttctgct tgttacctaa
     5161 attaaatcac aactaaaggt aacagtcaac aaagatttgt tactaatcta attttctatt
     5221 ttttcgcctt ccaaatcaag aaaaccgaag gtaacgtctt atcctagtgt gatacctcaa
     5281 atgtccactt ttgttacctt tgctgtccaa aagtgttacc cttactgtcc acttttgtta
     5341 cctttactgt ccaaaagtgt tacctttata cccctttaat acttactctc ccaagagatt
     5401 aagcctcccc taacagatat attaacagat atttaacaga tatattaaca gatatttaat
     5461 aaaggaggat tactcctctc ttatttcaaa atcttaaaaa taaaaaatct taaaaaaaac
     5521 taacactatc taggagttta ttttgcttgt gtaactttg
//
LOCUS       DAYYCY010000153         5538 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000153 DAYYCY010000000
VERSION     DAYYCY010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5538
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.154"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..744)
                     /locus_tag="ACHAZB_002337"
     CDS             complement(<1..744)
                     /locus_tag="ACHAZB_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element ISEfa7 family transposase"
                     /protein_id="HFM8614962.1"
                     /translation="MQGVIAMMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFE
                     FIYPIVEATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGYS
                     FEEKIPHFSTFGKNYVRRFRETTVFEDIFAYILEQAVKAGFVTEDNLYLDSTHIKANA
                     NKHKFTKEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKLKERKISKADPE
                     SGYYVKGEREKQFAYSAHTSCDDNGFILST"
     gene            complement(716..1915)
                     /locus_tag="ACHAZB_002338"
     CDS             complement(716..1915)
                     /locus_tag="ACHAZB_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFM8614963.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIEGKVLSMYARGMSQCDIAATVEDIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKVGGVEDVFFISMDGVSGLKEGAKAIFPSVIVQRCIVHLVRNALRYLPSK
                     DYKEVCRDMKKFYSASSLNAAHAAFDSFQNRWSHYSGAVDVCKRNFSHVEQLFDYGSA
                     IRKIMYTTNAVESIHSSFRKVTKKGVFSNENAQTVDKVHFMDDVYSLFFYNRFMKTNA
                     RSDSYDV"
     gene            complement(2045..3421)
                     /locus_tag="ACHAZB_002339"
     CDS             complement(2045..3421)
                     /locus_tag="ACHAZB_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8614964.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(3421..4077)
                     /locus_tag="ACHAZB_002340"
     CDS             complement(3421..4077)
                     /locus_tag="ACHAZB_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8614965.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            complement(4087..5364)
                     /locus_tag="ACHAZB_002341"
     CDS             complement(4087..5364)
                     /locus_tag="ACHAZB_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8614966.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            complement(5383..>5538)
                     /locus_tag="ACHAZB_002342"
     CDS             complement(5383..>5538)
                     /locus_tag="ACHAZB_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFM8614967.1"
                     /translation="DKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPVDA
                     ISYGVRK"
BASE COUNT         1713 a         1024 c          715 g         2086 t
ORIGIN      
        1 tgtgctaaga ataaatccat tgtcgtcgca tgaagtatgg gcagaataag caaattgttt
       61 ttctcgttcg cctttgacgt agtagccgct ttccggatca gctttactta tttttctttc
      121 tttcagcttc acttcctttt ctaagtccaa agtaaaaggg cgttttccag cctctattcg
      181 ttgacgattg atctcatctt ctaattcatc ctgataggct ttcgcttcat catgggtcat
      241 ttctttcgta aacttatgtt tattggcgtt agctttgatg tgggtagaat ccagatacaa
      301 attatcctct gttacaaaac ctgctttaac tgcttgttct aaaatatagg caaaaatatc
      361 ctcaaacacc gtcgtttctc taaatcggcg aacataattc tttccaaaag ttgagaaatg
      421 ggggattttc tcctcgaagg agtagcctaa gaaccagcgg taagcgacat tggtatcgac
      481 ttccttaatg gtttgtcgca tggaacggat accgaataaa tactgaataa acaccaattt
      541 gatcagaatc actggatcga tacttgggcg acctaaatcg gagtaagtgg cttcgacgat
      601 tggataaata aattcaaaat caagcgcggc atcaatcttt cgaacgagat gatcttgagg
      661 aactagatca tcaatcgtaa gcatcgcaaa ttgatttcta ccattcgtat tgtttttaga
      721 catcatagct atcactcctt gcattcgttt tcataaatct attataaaag aaaagactgt
      781 agacatcatc cataaaatgg actttgtcta cagtctgcgc attctcatta gagaacactc
      841 ccttttttgt gacttttctg aagctagagt gaatactttc aactgcatta gtagtgtaca
      901 tgatttttct aatggcgctg ccataatcaa agagttgttc aacatgagaa aagtttcgtt
      961 tgcatacatc tactgcacca gaataatgag accaccgatt ttgaaaactg tcgaaggcag
     1021 cgtgtgcagc gtttaaagaa gaagcactgt agaatttttt catgtctcga cagacttcct
     1081 tataatcctt acttgggagg tatcgtaacg catttcgaac aagatgaaca atacagcgct
     1141 gcacaatgac agaaggaaaa attgcttttg cgccttcttt gagaccggaa acaccgtcca
     1201 tcgaaatgaa aaaaacatct tctacaccac ctactttcag ttcatcaaat acttgcatcc
     1261 agcgattttt agattctgtt tgattgagcc ataggcctaa aatctccttg tttcctttaa
     1321 gatcatagcc aagaatggtg tacacagcat attctttagc ttcatagttt tcgcgtaaag
     1381 tgacgtacat acaatcaacg aatagaaatg cataacactt ggctaatggc cgagcttgcc
     1441 attcttccaa ttcaggaagg acggcatccg tgatatcaga aatcatttcg tgggaaatat
     1501 caaagccgta aatatcttca acggttgcgg cgatatcgca ttgactcatt cctcgtgcat
     1561 acatggaaag aaccttacct tcgatgtcgg agacatctcg ttttctatta ggaatcaact
     1621 caggttcaaa agaagcctcc cggtctctgg gaacatcaat gtttacttta ccaaagctcg
     1681 ttttaagcgt cttattgcca taaccgtttc gtcgattgtc atgtttcttt ggctctttag
     1741 aatgggcgtc ataccctaaa tggttattca attctccttg aagcattttt tcaaataagg
     1801 gaccaaacac atcttttaaa acatcttgca tatcctctac agattcgggt tgataagtat
     1861 tcaaaatgga ttcagctaac ttctcagcat caggatttct tttctttcta gccatcgtgt
     1921 aatcacctct tgatcttatt ttagaaaaat aaaaaccgtg aagcaaccac ccgcctagga
     1981 ttgatggtta cttacacggt ttatattaca ctctcatttt tggtgctaaa aagcaccgaa
     2041 aattttaatt attgtcaatt aataattctt ttggattcct tcttaatata ttgaatgaag
     2101 aaataatcaa tgatacaaga agtactaaac tcataaatat aactacataa atcatgaatt
     2161 taggtaatac attgatatct aaacttgata atgtcttgtt aaatccttca gcttctgccc
     2221 caccacctaa ttggctagat gcagattgtt tagctatttg tttagctata tcaccagtaa
     2281 ccttattcaa tatattattc ccaagcttgt cagctgtata ttgagctaag aaataggaac
     2341 caacgaatgc ggggattgat ataaatacca tctcaattag gaattgacca aaaatttcta
     2401 attttgatat acctaatgat agtaatactg ctatttcttt ctttctagca ttcatccata
     2461 agaataataa taatgaaact acaactcctg caaatattaa tgagccaaaa aataatttat
     2521 ttgaaattga gtaaatacct gatatagatt gttgtaatgc agggtaattt gatgaacttt
     2581 taatcaaatt atattctctc caatttatat ctaatttccc aagatctttt attacactat
     2641 caagattctt atcacctttt acaaagaatg ttgcatcttg gtatacagct gtatcttcgg
     2701 tattaccata aactttagca gctgtatgaa tatcagtgat taatgtattt tcgtatagtt
     2761 cttgtgctgc acttactcca ccgctattat gcccatcgaa tagaccttta atttctactt
     2821 ctactgtttc atctgcacct ttctcattat cagcatcaaa taaattagat tttattttta
     2881 tcttatctcc aactttaagg ttattttttt cagctaaatc tttatgcatt aagattttat
     2941 ttttatcttc attttctaaa tgctttcctt ctactaattt atatgcttct gatacaaatt
     3001 ttgtttcttt tgttgagtcg ttaaccccag ttaacataac tgttctttta aaattctttg
     3061 ctctttcagg tgattgattc gcaagagtct cttgagtttc aataatatca taatcaacta
     3121 aatctgcaac actgtttatt ctttttacat aggagtctat actatctgtt tgagatatct
     3181 ttttaatatc ttcacctttt acatttcccc cacctctagg tgttcctgga tttacttgtc
     3241 tatttatctc catagaaaaa ctatttgtta tattagcaaa tgtttctttt gaagctctgt
     3301 ccgtagcatc tttaatagat aaactaatga gacttaaagt tgacatagat aaaataacta
     3361 atatgataat taatgatttt aaactttttc tagttacata agcaaatgca ttttttatca
     3421 ttattcaacc tccaaattca ttttatttac ttttttcaac tttttaccac ttagttctaa
     3481 aatgatatcc gcagaatctg ctacttcctt actatgtgtt acaactatta cacatttatt
     3541 tctatcttta gctaatgtct ttaatatatt aattatttct ccagcagtaa cactgtctag
     3601 attaccagta ggctcatcag ctagtattat tggagcatct gataccaatg ctctagcaat
     3661 agctactctt tgttgctgtc caccagataa tttcataaca tttcttttta tttgtttttt
     3721 atctaaacct agttcaaaca agatactttc atctgctgat ttatttacta atctaatatt
     3781 ttcaattggt gataaataat ctattaaatt atagttttga aatactaaag atatattatt
     3841 ttttctatga ttactatatc ctttattttg tatatcttca ttcttaaaca atatttttcc
     3901 tgtttgaggt ttatcaagtc cagcaagtaa ggaaagaagt gtagattttc ctgttcctga
     3961 ctttcctact atcgcataaa acttcccaag ttcaaatttt tgatttactc ctgacaaaac
     4021 tttttcttta gaatttgcat aactatatgc tacattcttt atttctaata tatccattat
     4081 tttctcctaa cttatttttg ataagatttc tttaggcttc ttgattaaca ttaatgaaga
     4141 agcaaataca actgataaaa caataatact tattaatatt aaataacttt gtccaagtgt
     4201 tgttatattt gacataaaac tactattatt tattaaactt ccaccggaaa tcattgaatc
     4261 ctctgagtta atgaatccat ctacaattac ttttagtaat acatttccta aaaataagga
     4321 agatattata cttggtatcg atatgaatat taactcgaat ataaattgca ttataatttg
     4381 tatcttagat gttccaatag ataagaatat acctatttca taaattcttt cccttaacca
     4441 tagaatcaag attaatgaaa gaacaaccat cccacctaac ataatagaat aagtcattac
     4501 ttttattata tattttattc cactcactga ctctaaagac tcttcaaacg cattagaatc
     4561 tttttcaaca aaatactttg actcatcaat tttaagctct ttcaatttgt ttaaggctaa
     4621 gtctgtagat tctgcactac cagaatacat taaaatttta tttgcaattt cattattttc
     4681 tgaattattt aatatctctt ggcttgttga ataatctaca aacaccatat tttcactaaa
     4741 atcagaagat aatcctgtat atgtttcctg ttttttacca gaaaagatcc ctataatttt
     4801 aaatttatga ctttttattt ttccactttt ttcaatatct agtaattcaa gatcaacttc
     4861 atcacctaat tttagattgt tttgtttagc aaattcttca tgaacaataa ttgaattctt
     4921 atcattttct tctatatttt taccttcttt aattgtaaat actccactac taaataaaat
     4981 atttctttta gtattatttg tagcttcgag tgaaacaaca ttcttaaatt cgtcagataa
     5041 atcttctcta tttattcttt gttctccgct aactacttta gcatctttta gttttgctaa
     5101 tccatcatat tgaattattt tttcttcaat ttcttttaat ttttcaatat ctttaaattg
     5161 attaacatta aaatatttac catctttttt tgttattgat attgaagaat tagaactttc
     5221 atataaagcc ttttctattt cattgcttga tttcattatt gttaaacaag aatacaagca
     5281 agaaagaact atagttaata tgataaaaat tattaaagtt ctatttttct ttcttgtaat
     5341 atatgctata gcatttttta tcactttcct aactactcct ctctattttc ttactccata
     5401 actaattgca tccactggac aagcattttt acatcgtcca catctgatac actccagatg
     5461 attgcaattc tcaactgaat ctttattcat ttggcaaact tttttacatt ttccacaatt
     5521 aatacactta tccttatc
//
LOCUS       DAYYCY010000154         5529 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000154 DAYYCY010000000
VERSION     DAYYCY010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5529
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.155"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..182
                     /locus_tag="ACHAZB_002343"
     CDS             <1..182
                     /locus_tag="ACHAZB_002343"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8614968.1"
                     /translation="KKRKNFAANDLLPLDFISEKDKNVMKNKDSIHSICLQFERVSVL
                     FKGMHETFLMYKMNF"
     gene            199..1488
                     /gene="tig"
                     /locus_tag="ACHAZB_002344"
     CDS             199..1488
                     /gene="tig"
                     /locus_tag="ACHAZB_002344"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFM8614969.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            1735..2985
                     /gene="clpX"
                     /locus_tag="ACHAZB_002345"
     CDS             1735..2985
                     /gene="clpX"
                     /locus_tag="ACHAZB_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HFM8614970.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVFNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            3068..3655
                     /gene="yihA"
                     /locus_tag="ACHAZB_002346"
     CDS             3068..3655
                     /gene="yihA"
                     /locus_tag="ACHAZB_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFM8614971.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(3729..3884)
                     /locus_tag="ACHAZB_002347"
     CDS             complement(3729..3884)
                     /locus_tag="ACHAZB_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPJ_0845 family protein"
                     /protein_id="HFM8614972.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            4020..5348
                     /locus_tag="ACHAZB_002348"
     CDS             4020..5348
                     /locus_tag="ACHAZB_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8614973.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
BASE COUNT         1932 a          914 c         1122 g         1561 t
ORIGIN      
        1 aaaaaaagag aaaaaatttt gcagcaaatg atcttcttcc attagatttt atcagtgaaa
       61 aagataaaaa tgttatgaaa aacaaggatt ctatccattc aatatgttta caatttgaaa
      121 gggttagtgt actatttaaa ggtatgcatg aaacgttttt gatgtataaa atgaatttct
      181 aaactcggag ggaattaaat gactgcaaca tttgaaaaaa aaggcacgaa tgatggtgtt
      241 ttaaaattta cgattgcgca agacgaaatc caaaaaggat taacaactgc gtttaataaa
      301 gtgaaaaaaa gcttgaacgt acctggattc cgtaaaggga aagtttctcg tcaagtattc
      361 aaccgtatgt acggtgaaga agctttatat gaagatgcat tgaatgctgt attaccagaa
      421 gcttatgaag ctgctgtcaa agaagctggc atcgatcctg tttctcaacc aaaaatcgat
      481 gttgaaagca tggaaaaagg tcaagactgg gtaatcactg ctgaagtaac agtgaaacca
      541 gaagtaaaat taggacaata taaagatcta gttgttgaaa aacaagaccg tgaagtaaca
      601 gatgctgacg tggatgcacg tatcaaacgt gaacaagaag cacaagctga attagttatc
      661 aaagaagatg ctgcagctga aaatggcgat acagtagtaa tcgacttcga aggctttgta
      721 gatggcgaag cgtttgatgg cggaaaaggc gaaaattact cacttgaatt aggttcaggc
      781 tcattcattc caggttttga agatcaatta gttggtcata aatcaggtga aagtctagat
      841 gtaaatgtaa ctttcccaga agattatcaa gcagaagatc ttgctggaaa agaagcagtc
      901 ttcaaagtga caatccatga agtaaaagca aaagaagtgc ctgaattaga tgacgaattc
      961 gcaaaagatg ttgatgacga agtatcaaca ttagacgaat tgaaagaaaa atatcgcaaa
     1021 gaattagtag aaacaaaaga aaaagcagcg gacgatgcaa aagatgaagc agcgatccgt
     1081 atggctgttg aaaacgcaga aatcgttgaa ttgccacacg tgatggttca tgatgaagta
     1141 catcgttcaa tggacgaatt cttgaacaac atgcaacgtc aaggtatctc tcctgaaatg
     1201 tactatcaat taactggttc tacagaagaa gacttgcata aacaattcga aggtgaagca
     1261 gaaatgcgca caagaacaaa tcttgtgatc gaagcaattg cagctgctga aaatcttcaa
     1321 gcaacagaag aagaaatcaa tgcagaaatc caagaattat cagaaacata caacatgcca
     1381 atcgaacaaa tcaaacgcgt gttgacagaa gatatgctgg aacatgatgt gacaatgaaa
     1441 aaagcaatcg aattgatcac aggtacagcg acagaagcga aagaatagaa atcaagtaat
     1501 aaatcatttc tgaagaagct acttctagaa aattgtttat tcgaaataag ggactgtgac
     1561 acgattttgt cacagttcct tattttttgt taagagtaaa aaatgcaaag gtataggtca
     1621 gttgatcctt tgttaaacac tttttctttt tactaaacta tgttatgata gtaaaagact
     1681 gtaaagtctt tgatagtttc ttgcatactt tttgcaagtt aaggggtgaa gaccatgtat
     1741 gacaatccaa gtagcaatga gaccgtacgc tgttcttttt gcgggaaaac ccaagaagaa
     1801 gtgaaaaaga tcgtagctgg tccaggggtc tacatctgta atgaatgtat cgacctatgt
     1861 aaagaaatca ttgatgaaga attttatgaa gaagctgtac gcgaattcac tgaagtccct
     1921 aaaccactag aaattcttga agtatttaat aactacgtga ttggacaaga tcgcgcaaaa
     1981 cgctcgttag ctgttgccgt atacaatcac tataaacgtg tcaatcagca agcacaagaa
     2041 aacacagatg atgttgaact gcaaaaaagc aatatctgct tgatcggacc tacagggtct
     2101 ggtaaaacat ttttagcaca aacattagca aaaacattga atgtaccttt tgcaatcgca
     2161 gatgcgacaa gtttgacaga agctggttat gtgggggaag atgtagaaaa cattttgctt
     2221 aaattgcttc aatctgctga ttacaatgtg gaacttgcag aaaaagggat catctatatt
     2281 gatgagatcg ataaaattgc gcgtaaaagc gaaaatgtat ctattactcg tgacgtttcc
     2341 ggcgaaggtg tccaacaagc cttgctgaaa attctagaag gaactgttgc aagtgtgcca
     2401 cctcagggcg gacgtaaaca tccacatcaa gaatttattc agattgatac gacgaacata
     2461 ttgttcatcg taggtggtgc atttgatggc atcgaaacga ttgtgaagaa tcgtatgggc
     2521 gaaaagacga ttggatttgg tacacaaaac caaaaacttt ctgaagacga aagtgtcatg
     2581 cagcatatca ttcctgaaga tttactaaaa ttcggattga tccctgagtt tatcggacga
     2641 ttacctgtta tggcagcttt agagaaattg acaacagatg atctggtacg gatcttgact
     2701 gagccgaaaa atgcgctagt caaacaatat caaaaacttc tttctctaga taataccgaa
     2761 cttgaattcg aacctgaagc gttgagagcc attgcgaaaa aagcaatcga acgcaatacc
     2821 ggtgctcgag gacttcgttc catcattgaa gaaatcatga tggatgtgat gtttgatatc
     2881 ccttcaaatg aaaacatcga aaaagtgatt atcacaaaag aagcagctga attgtctggt
     2941 gagccaacag taatctataa agattcagag aaaaaagcgg gttgatgaaa agaggctgcc
     3001 gtaaaagtca ttttggcttt tgcgacagcc gcttgcttta ttcatcacct tgttaaggag
     3061 agagaccatg aaagtccatc aagctgaaat tatcataagt gcagtggcac caaaacaata
     3121 tccagataca caattgccag aaatagctct ggcaggacga tcaaatgtag ggaaatcttc
     3181 ttttatcaac acattgattg atcgaaagaa tttagcccgt acttccggta agccaggaaa
     3241 gacccagaca ttgaattttt atttgatcga gaacgcatta cattttgtcg atgttccggg
     3301 atatgggtat gcaaaagtat ccaaaactga gcgagcaaaa tggggaaaga tgatcgagac
     3361 atatttaacg actagagacc agctacgtgc ggtcgtttct ttagtagatt tgcgtcataa
     3421 gccttctcaa gaagatatcc agatgtatca atttttgaag tattatgata tccctgtaat
     3481 tgttgttgcc actaaggcag ataagatccc tcgtggtaaa tggaacaagc atgagagcga
     3541 aatcaaaaaa gcgttggatt ttgatcgcaa tgacacgttt attctctttt cttccgttac
     3601 aaaacaggga aaagaagaag cgtgggcagc aatcgaacaa gcaattgaat cataaaaaga
     3661 ataaaaaaga cttaccagtc aaactctttc ataaaagttt ggctggtaag tctttttttg
     3721 agcttaactt acttttgttt cttgtctttt ttcttttcat tttgtggctg tggttttaga
     3781 aatcgatcga tctcttcttt tggtgcaact gttttttccg gatcgacagc aaatgattcg
     3841 aaaagtttat ctaaattatc tgtacgttca aatttcaacc ccattgtaaa cacctctttg
     3901 tttcttttta cttagtctat catgtaatcg tccaaattgg tttctaagac ataaagtttt
     3961 agtttttctt tatcattggt tataattaaa ttataaaaag atttgaaaga ggtgagaaaa
     4021 tgattggcga agataaatta ttgatgacta tccagctcac agacttaaat gaagaagaaa
     4081 aaaaacagga aataagcgat tgggctattt tgacacgtct gttgatccag ccaacattta
     4141 tccgctcttt cacacaacaa gcggatccgt atgatcttcg aaagcttcaa gaaaaaataa
     4201 tgctagcatc ggtaagagac gaaagttggt tagttgtagg aaatgacgag aacgaatgta
     4261 gttttcgctt ggaagataat caattattga taaaaaatgt attgtctatc tctgttttca
     4321 aagaaaagca atttttgatt cgagacttca ttcagaaaaa aatgattgct catggggtgt
     4381 tcgcctatat gcgagcttat agcgagttta tctatcacaa tacaaaacag atctctcaac
     4441 ggctattgtt tgagaaaaag gatgaaatcg aacatttacc taaaatgaaa caacaaaacg
     4501 gggaagtagt agtcgattgc aaccaatttc caggatacga tcttttttac caaggtttat
     4561 gtttcacttc gtgttgggaa atgtattata gtcgctatta tcatcaaatc atccctaaac
     4621 cgatttttct cgacatccaa caagtagaaa aagtgaagga attagaaaac gaagtcatat
     4681 gtatccagct ttatcgagac ccctttaact ggcaaaagcc aaacaatcaa atgtttcaat
     4741 cctattttag ggaccagctt gggtttgatc atcttgcttg ggacaatggt gtcggtttat
     4801 tgaaaccgcc attcgttgaa tacatttaca ctgaccatac gatccaatct gtccagtatc
     4861 agaatgcaca aatgcaacct gttcctaaaa agaatgcttc attttttgta acaaaaagtt
     4921 atgatatcca acaaggagat tataaagaaa ggcgtgttcg cggaacacta aatgctcagg
     4981 cgtattttcc ttgggttgac gagaatcgat cacgcatgat gtgctataaa gttatcgatc
     5041 cgactgttgc tttggataat ggaatcgaag cttattgtta ttatattagg gaatatctag
     5101 aagtagaggt aacagatgaa aaatatcaag agtatctttt atccttaaga atctatgttc
     5161 cttcagaatc cctgtcagaa ttaccgttaa aggaaatcaa acatagactc tctgatgtta
     5221 ccttcaaacg aataaagaaa aagcggcgaa gtatccaatt cgatgtcaag aaaggtgagc
     5281 agcatctgcg tgtgcaattt ttagattacc gagaattaga acaattgatt actttacaaa
     5341 aaatatgagg tgaagaaaat ggataaacaa ctctattatc gaataatggt tccggcatca
     5401 ttattatccg tctgtctctt ttttgttcct aattttccta ttcgatcagt catcggtttt
     5461 ttggtcatgt ttggcgggat gggactttat tattatcttc ttcataagga aagaaaacct
     5521 gcttctgtt
//
LOCUS       DAYYCY010000155         5359 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000155 DAYYCY010000000
VERSION     DAYYCY010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5359
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.156"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            63..1808
                     /locus_tag="ACHAZB_002349"
     CDS             63..1808
                     /locus_tag="ACHAZB_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HFM8614974.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(2088..2525)
                     /locus_tag="ACHAZB_002350"
     CDS             complement(2088..2525)
                     /locus_tag="ACHAZB_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFM8614975.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(2509..3882)
                     /locus_tag="ACHAZB_002351"
     CDS             complement(2509..3882)
                     /locus_tag="ACHAZB_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8614976.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(4204..4692)
                     /locus_tag="ACHAZB_002352"
     CDS             complement(4204..4692)
                     /locus_tag="ACHAZB_002352"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8614977.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(4714..5316)
                     /locus_tag="ACHAZB_002353"
     CDS             complement(4714..5316)
                     /locus_tag="ACHAZB_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFM8614978.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         1760 a         1136 c          933 g         1530 t
ORIGIN      
        1 tcagctagat tatttgtccc gttttttata aaaagttatg ataaagaaaa ggagggatga
       61 tgatggcgca aacaaatcca cgcagccgct tggcagaaat cattcgagtg ttcatcaatt
      121 acaatgtagt gccaaacttt gtacagcaaa aaaatcccga acaggtaaaa aaagcctttg
      181 aagaactagg ccccaccttc atcaagatag gacagatgct ttctgttcga gaagatttgc
      241 tttcttctgc cttcactcaa acattcaaaa cattacaaga tagtgtacct agcgacacat
      301 tttcaacggt caaaaagacg atcgaaacag aactttctct ttcattatca gatatattcg
      361 atgatttttc aaaatcgcct tttgcctctg cttctatggg gcaggctcat cgtgccaagc
      421 tgaagaacgg tgacgcggta gtcgttaaga tccaacaccc taacatcgcc gaagagatcc
      481 gacttgatct tcaactattt gaacgagcga tccctttgat caaatacatt cctgaaacta
      541 gcgtagttga tctaaaaggg gtactccaag aagtaaaacg ttccttgatc aatgaaatgg
      601 acttcttaaa agaaagtcaa aatggcgaac aattctatca aaagaacaat ggctggaaag
      661 agatccgcag ccccaagatt tatgacgctt tttgttcgaa aaaagtgatc gtgatggaag
      721 aaatgtctgg taaaaacttg aatcatttaa tgaacgccga aaacaaaact gaaactttta
      781 tcactggaat tcaaaacaag cagctgaaac aagaagttgc aaagctttta gttgagaact
      841 ttatgaaaca ggtgtttgat gatggttttt tccatgctga tcctcatcca gggaatctgc
      901 tattccatgt actaacaaaa gaagaacaga cacaagctag ccgaaaaact gaaacagtcc
      961 atgaaaaaga gttcggttcc tttgcttttc gagcctccac ttctgcagaa gaccctgttg
     1021 cgccatatac catcaactac atcgattttg gtatgatggg gcatctttct gcaggactta
     1081 ggcaaaaact cacacaagcc gttttagcgc tgtatacgaa agatgcttat cgaatcgaaa
     1141 aagcggtcct tcgactttgt cagcaagaag gctcgtttga tgaaagtcgg ttccaccaag
     1201 aactgacaag ttttcttgaa cagtattatg attctcctat tgatgaaatc aacttgcaag
     1261 aagtattcac acatgtcgtg acgatttgtc atcaaaataa tctgcagttc gatcgtgata
     1321 tcacgctttt gctgaaagca tttgggacat tagaaggggt gatccgcgtc ctcgatcctg
     1381 aagtttccct catggaagtc gcttcgcctt ttgctcaaca ttattttctc actcatctgg
     1441 atgtagaaga tacattgaaa caaagcggat tagacttatt ggaagggatg aaggctgcgc
     1501 ctaagatccc tcaacagctc caccatctat tagaaatgtg gacttcggga caaggaaaag
     1561 tcaatttgga attgaaaaag caagacaaat tattgagcag gatcgaaagt atgatcaatc
     1621 ggcttgtttt cggtatgatt ttagctgcat tgatcgtagg gtcctccctg cttgtccaag
     1681 cagcacctgt agaaaacgca gaggtcgttt ccttattagg gatatttacg tatgctattg
     1741 ccgcattcgt cattattttt ttggcaatcg atgcattgat ccaaatgtat aagaagagga
     1801 aaaagtagaa aaaggttgtg acagaaagaa tcagcttcaa gaaataagaa ggggttttac
     1861 ataaattgtt tctcaaattt ttgtgaaaat tcggcttatt tttcgaagag gctacttctg
     1921 aaacaccgag aataaaaaat gtgaaaaagc tgttctgcac ccagtaataa gaacaatcaa
     1981 aaagggaaca tggaacacat ttatcctgtt tccctgttcc ctttttgatg tattcgagca
     2041 taaataaaat accttcgtta cctactatct tctgtacttg gtaaacctta gtcaattgcc
     2101 ttttttaacg cttgacgaaa agacatatga tctttctccg taaagggttt tggacccttt
     2161 gtccttatgc cagcttttct taactttcca ctatgataac gttgttctag aagcgtgccg
     2221 atattcgctt cgtcaaattc ttttccagag tgatggaaca ctcgcacacc cttcggcaat
     2281 acaagtgaag ctaaaccata atcttgcgtg atcaaaaagt cattcttctt aaccataccg
     2341 acgattttat aatccgcact atcagctccg cggtctacgt agacgatttg gacatgatta
     2401 gcgtattctt tggtcgtata atggtccaca ctggtcacga taaccacgtc aagaccatag
     2461 gattctccta attttatgac atcttctttt actggagaac catctccatc aatgaatagc
     2521 ctcattcttt ttcgccttct tccataaatg ataaacagtt actaatccgc tcagtaaaag
     2581 accaacattg atccattttg ccaagataag cagttgtaca ctttctatcg gcactgattc
     2641 atggttcagc gtgatcaata atagatggat tggctctccc atttcttttg ctaaaacaaa
     2701 caaaagcgtc atcaataaaa actggtggat cacactccct ttattttgag tagtatactt
     2761 gacaacgatc cggcgatcca ttggaatagc tgtatcagtt tgataatatt ccttatttag
     2821 ccagctgcct gttactacgt gccagaaact aagcagaaag acacctatcg caaaaccatt
     2881 tggaatcaag aggaccaata aagaagaact cagtccaagc aattgcatag ccaatggaga
     2941 ccatgttaaa cgtttcaaaa tccatggtcc gactattttt gctgcaatca agcctgctgc
     3001 ataaggcaaa tacataaaaa tcgaaagttt ttctaatccg tacagtatgc caacataaaa
     3061 actgctaaat aaaaaaagga aattccctag cattccattt agaaaagtaa acagatttag
     3121 ccagcttggc aacttccatt ccttcttctg gtgtcctaaa acaaacacga acaagatgaa
     3181 cagcagcaag aaaccataaa tagcaagatc aaaaagcctt tcatcgaata acaatcgggc
     3241 atttctcaaa agaaacaata agccaaagaa tatcaaaaat aaaacaagct ctcgcgtagc
     3301 taccagattt cttttgatgt ctttgaaatc cagttcgtaa cgcggataat gactaaccgt
     3361 atggtaggcc atgacgtaaa aaagtgtata aatagtaaag cttgctgcaa tctgagtcgg
     3421 tgcaggcaac tcaatcgctt gatacaggag aaatagccag atagctgcaa ataagtactt
     3481 tttcccatcc atattgataa aaccttgcgt cttttcgtga aaattgaccg tggtattagc
     3541 aggtacaagc catgctgcac tcagccctag caaaacactt gaaaaataaa ataaaggaaa
     3601 ataaaatacg cctaatattc caagcaatga accagcacca ccaatcagca gagcgatcat
     3661 cagcaacgtg taactatcta gaccaaatcg tagactgttg actaaaaata gtccagtcat
     3721 ccgaaaggca taaaacagcg taaatggcaa aatcgtcagc caattatgcg agtctgttag
     3781 tcccaaaaat aaaagataag gaataaaaat aaccgtgtta cttaagataa agggcgttgt
     3841 ttgtgtaaaa ttacgcccaa atcgtttgag tttttcgaac atgacaatgg ctccttaaac
     3901 agtcttataa ctaatctgct cttcgatttt agcattaaag gggaaataaa ccaattttac
     3961 agaatatatt cttgtaaaaa aaaatacaga aagaagcata agcttctttc tgcattttta
     4021 cggtacttga ttactcaagc agacaagccc ttctccacaa aagtcttgcc cgacacccct
     4081 gcatccggca ggtgataaag atagaaatca attatcctgt gcctccaaaa tcaattagga
     4141 gctctgtaaa ccagagtcct tctcgacccg tcgcataaat ttactttaac acagttccta
     4201 tgtttatgcg agagcttttt tcattttata atcgaccatt tgaaatcctt gaccgatatc
     4261 tgaaataagt tcttctgtaa tctcaaatcc ttcatgctta taaacctcga ttgcttgatg
     4321 attcccctga ttcactcgca aaaagatctc tctttccttt gtttgcgcag caagttcttc
     4381 ataccagcga aaaactgaag aagtcaatcc ttgaccacgt actttattta gcaaatataa
     4441 tttacttaaa taaatttttc catttatctc ttcataagca gtgtaaccga cagtcttttc
     4501 ttcgcttttt aataaaaagt aggacacacc ttctgtcagt tctttttgaa tctgtttttt
     4561 tgactgataa gttttcagca tgtaatccac ttgtttttga ccaatgatcg gcacaaaaac
     4621 ttccggccat acttcttgta tgactgccat cagctcatcc agttcatttt ctgttacttt
     4681 tattttttcc atacacccac gtcctttttt acattataaa tgaccttgcg cttgatggaa
     4741 taaaaagagc atgatcatgc tatacacacc caccgaccac ggtttcaaga aaatcacaat
     4801 ctgcataaat tccctaaaag ataatttcgt caaaccagct actagacaga tcaagtcgtc
     4861 tggcgcaaag ggaagcagca aggtaacaac tagtactttt ttaatatctt tcgtttttct
     4921 tgtcagcaat tcatcaaatt tctgatactt ttttggcgat aaaataagct ggacaaaggc
     4981 tacgccatag taacggacaa gcaaaaatcc gattgcttcc ccaatcacta ggccaataca
     5041 actatataaa agcccaaatc cattgccaaa catcagcatg cccgctactg aggagatccc
     5101 tcccggcaaa ataggaatga tcacttggat cacttgcaac agaacaaata tcatcactgc
     5161 atacttgcca aattgctgga taaacgcttg caaagacgtg ctcgatcgaa aaatcccatg
     5221 atgataagca taaagaatca acacaaagaa aaaaaacaga ccaataatgg gcatgatctg
     5281 aagacatttt tgtaaaactg atcctttttt agacatctat attccccaca tcttttttca
     5341 tttattaaaa ctgcctcct
//
LOCUS       DAYYCY010000156         5351 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000156 DAYYCY010000000
VERSION     DAYYCY010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5351
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.157"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(107..1255)
                     /gene="nagA"
                     /locus_tag="ACHAZB_002354"
     CDS             complement(107..1255)
                     /gene="nagA"
                     /locus_tag="ACHAZB_002354"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFM8614979.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1331..2167)
                     /locus_tag="ACHAZB_002355"
     CDS             complement(1331..2167)
                     /locus_tag="ACHAZB_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8614980.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2351..3568)
                     /locus_tag="ACHAZB_002356"
     CDS             complement(2351..3568)
                     /locus_tag="ACHAZB_002356"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFM8614981.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3717..3878)
                     /locus_tag="ACHAZB_002357"
     CDS             complement(3717..3878)
                     /locus_tag="ACHAZB_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8614982.1"
                     /translation="MEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGIAMDWAA
                     NLWNDYHDN"
     gene            3951..4034
                     /locus_tag="ACHAZB_002358"
     tRNA            3951..4034
                     /locus_tag="ACHAZB_002358"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3985..3987,aa:Leu,seq:gag)
     gene            complement(4274..>5351)
                     /locus_tag="ACHAZB_002359"
     CDS             complement(4274..>5351)
                     /locus_tag="ACHAZB_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8614983.1"
                     /translation="KYVFEKINVKNLLLVCSIYCLIPLTVMGETGWRATTLNYQWPVA
                     FSLLTFYPFFQLLRGEEINRKIYWVSIPLLIFLTNQEQVNACFFVLTSIVSLYLIVNG
                     RYNYKLSVFSIISLAELIFSLTTPGNALRAAHEINKWFPEYKNFNFLNKLDLGISSFG
                     KPFFLDMNILFLLLFFLIFLLTYRKCQNYYVRILTALPFFLNLIIYFGNTMGQSFTYV
                     NGNKRAMIWSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLIVGIYLSFDNKK
                     TSIFLVILMIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMAVKELMKSMSVQ
                     KNEFMQFGLTVLGICTFIITVINR"
BASE COUNT         1670 a         1085 c          844 g         1752 t
ORIGIN      
        1 aattaaagaa acttttgaaa cttatgtaaa gtgattttta ctagaacaca aaaaaacctt
       61 aaaaccaaac ttaagaagtt tagttttaag gttaacttaa attatattat gcgtggaaac
      121 gttccacgcc atctagataa gttgcttcca atgtcatatc agggttcaat acgataaagt
      181 ctgcatcgcg tccttgagcg atttttccac atgtatcatc gattttacag ctgattgctg
      241 gaatcagact agccatcatg actgcttgtt ctggtgttgc taatccccag tctacaacat
      301 ttttaattgc ttctttcaat ttcaatatac ttccagctaa gttgccagat tctagacgtg
      361 ctgttccttc ttttactact actggaaatt cgcctaaatt gtaatttcca tcaggcatcc
      421 caccggccat catacagtct gtaattaatg cgacatgatc atgtcctgct ttttccatta
      481 ggattcccgc agcatttgga tgtacatgat ggccatcaca aatcaattca gaaaagacat
      541 gttctaaaga taataacgca cccaccatac ctggttcgcg atgattcaat ccacgcatac
      601 cattgaaagc atggacaaat acacttgcgc ctgcttcaac agctttttgc gcttcttcta
      661 gtgtagcatt actgtgtcct agagaaacaa ctactccttc ttcagtcact tgtttgacaa
      721 actcttctac accttgacgt tccggtgcta aagcaatttt tttgattaaa ccacctgaag
      781 cttcttgcca ttcatggaaa gtatccaaat caggatcccc gaaataactt gggttttgtg
      841 cccctttgtg ttcttctgtg aagaaaggac cttcaaaata gattcctttg attttagctc
      901 ctggtgcttc ctgatacatt ttgccgattg tctcagctac atcttttaaa cgttctttgc
      961 ttgacgtcaa tgttgttggt aaaaatgaag tcactccaca agataacaga ccttcagaca
     1021 tcactttgat gccttctgca tcattatcca ttacatcatg attcatatag ccgtgaatat
     1081 gtgtatctac caaaccagga gcaatccatt tgccgctttg gtcaatgatg ttgacttctc
     1141 cctcaggtag ttctttggtg tattcaccaa aaagtccatc ggtaatttca agatagcctg
     1201 catgtttgac acctgacgct aaaaagaatt tgtcagcata gataaaagtt ctcattctat
     1261 tctctccctt tgtttttcta cctctaaagt actcttattt agagaagaag tcaagaatgg
     1321 tctacaccat ttacaaattt tttgaaattt gtttcacagc ttgtgctaag ccttcataga
     1381 atgctgctct ttctggttct tctttacgct tgtatattaa gcttttaagc acaaataaat
     1441 cattgaggta atagtagcta ttcttctctt gagcccaata gattccttga ttcacctgtt
     1501 ctaacgcttc gtcgatttca ttttgatgga ataagaaatt tccataatta taaaaaattt
     1561 gcgaaaattc actttttgat cgttcatcag ctgattgatg cagcagttgg atgcttcgac
     1621 tcaaatagta gcgtgcttca gccgtttttc ctgccacacc atatattttt ccgatacaac
     1681 taatcagttg tatttctgtg tcagaaacat ggattctttc tttttggcta gagtagctta
     1741 atgcttcttt caaataataa agggactgag agatattttg acgcaacgtg aattcgcaaa
     1801 caccgagata ataataatag cgttgaaagt cagttgcttg atacagattt tcagaaatat
     1861 cagaagattt tagtacttgt tctaacttcc gataatcacg tttttggaaa caactatcca
     1921 acagttcaaa taatttatcg tttgtccgag tagtttttcg agagagattc atcacatgat
     1981 caatcgttac ccctagccgt tgacaaatct gcatcatcac caatacattc ggcagttcct
     2041 cattattttc gatccgactt aatacacttt gtgaacaaat atcatgcgaa agcatttttt
     2101 gcgtcatttt ttgttctttt cgaatctctt taatgatcgt tccaaaggac tcgattaacg
     2161 tttccataag tatcctcccc aaaatatgcg tattcgcatt ctattcgtat tttactttag
     2221 gaagtcgcaa acttcaagaa aagacttgca actaaataat atactttagt tatttaagaa
     2281 aactccacta gaaagagagc ctcagtgtgt ttgtctgtgg ccctcctact gaaaaatttg
     2341 gtttttaaaa tcatttttgg cgataggttg ctaggaattc tctcattttc ttagctgttt
     2401 gtttcaaatc atccatttta ggaagataaa cgattcgaaa atgatctggc tggttccaat
     2461 taaatccacc accatgtaca agtagaatat gatgttcatg taaaaagtcc aaaacgaatt
     2521 tttcatcatc caaaatattg aatcgctttg tatcaatttt tgggaatata taaaaagcag
     2581 cttttggttt tacggctgaa agaccaggaa tatcattgat tgcattatag atatattctc
     2641 tttgttcata gatccttccg cctggcagga gcagctcatc gacactttga taaccgccta
     2701 gcgcggtttg gataatttgc tgagacaata cattggaaca caaacgcatg gaggacaaca
     2761 tattcagccc ttctatatag cctttgacgt gtgacttatc tccacttagg accatccagc
     2821 ctactcgaaa tcctgctaca cgatgagatt tggatagacc gttcagcgta acaacgaaca
     2881 aatcaggcgc taatgtagca atcggaatat gttctagacc atccatcact aagcgatcat
     2941 agatttcgtc ggaataaata atcaggttat tttgtcgagc tacttcaacg atttcttcta
     3001 ataattcttt tggataaagt gccccagtag gattattagg attaatgata acaatagctt
     3061 ttgtccgact cgttattttt gatttgatat catcaatatc aggataccat tctgcctctt
     3121 catcgcaaat ataatgaaca ggtgtgcctc cagctaatgc aatagaagcc gtccacaacg
     3181 gatagtccgg cattggaacc agtacttcat ctccattgtc cagcaacccc tgcatacaca
     3241 tcgtgatcaa ttcgctcact ccgttgccgg tgtagatatc attgatcgtc acattcggaa
     3301 aatgtttcag ttggcaatat tgctcaattg cttttctagc agaaaagatc cccttggaat
     3361 ctgagtagcc ttctgagtca cgggcattca tgatcaaatc acgaatgact tcgtttggtg
     3421 cttcaaatcc aaaaggtgcg ggatttcccg tgttcaattt taaaatacta atgccctctt
     3481 cttgcatccg ctccgcttct tctagaacgg gaccgcgaac atcgtaactg acaccttcta
     3541 atttgttgga tttttcaaat tctctcatca cttagccaat ccctcatttc aaaaagttag
     3601 aactttccaa attttttgtt atttactatt tcaatatcat actccatcct agaaaatttt
     3661 gccacttatt taggaaaaac gtaagtatta aaaaaacaca aacaagcgtt gagaacttaa
     3721 ttgtcatgat agtcattcca tagattcgct gcccaatcca ttgcaattcc acaaatgatt
     3781 cctagcataa aaccaagcaa aaaacgtccg aatgcaagtc caatcaatac acctaagatc
     3841 attcccagtc ctgaaaattc tccgtcatat ttctccatct taataagtcc tcccattttt
     3901 gagcttgaca actatgtaaa acctcgctat cattagtgtg ttattattac gcagatgtgt
     3961 tggaattggc agacaagcat gattgagggt catgtgagcg ttagctcgtg tgggttcgag
     4021 tcccatcatc tgcactacaa ctcgtgaagt taattttgct tcacgagttt ttttgtttga
     4081 acgtttcttt atgccattat atacatatca ctgtatcctt tacaaaaaga acgtacaatc
     4141 atgtaaacag cctgaggtga gtatatgaag gtcggaaaca agctactcgt agaaacttgc
     4201 aagtgttaaa acaattaatc gaaaaacgag taaaatccct cacctaatac taaagcgagg
     4261 gattttttgt atattatctg tttatcaccg ttattataaa ggtacaaata ccaagcacgg
     4321 ttaatccaaa ttgcataaat tcattttttt gtacactcat gcttttcatt agctctttaa
     4381 ctgccattag tactaagatg gcgataacaa cataaagtat atagtacgtc cgcattccgg
     4441 aagcccatac tgtgggagaa aatcccataa ttaaccttga acaaaatccc attatcatca
     4501 aaataactaa aaaaatagaa gttttcttat tgtcaaaact taaataaatt ccaacaatta
     4561 aacatagtaa taatgccaag actactaaag tagctatcca agtacctgga taaaaaagtg
     4621 acagtttcgt tcctaactct gtaaagagat tattcaggtt actactactc caaatcattg
     4681 cccttttatt tccattgacg taagtaaagc tttgacccat tgtattacca aaataaataa
     4741 tcaggtttaa gaaaaacggt aatgcagtca atatacgcac ataatagttt tgacatttcc
     4801 tatatgttag aaggaaaatt aagaaaaata gcaataaaaa taagatattc atgtctaaaa
     4861 aaaacggctt tccaaatgat gaaataccta aatctaactt attaagaaag ttgaaatttt
     4921 tatattcagg aaaccattta ttaatttcat gtgcagcacg taatgcattc cctggagttg
     4981 tcaaagagaa aattaattct gctaagctta ttattgaaaa tactgataac ttataattat
     5041 atcgtccatt gacaatcaaa tataaactaa ctatacttgt tagaacaaaa aaacaggcgt
     5101 tgacttgttc ttgattagtt aaaaatatca ataaaggaat acttacccaa tatatttttc
     5161 tatttatttc ttctcctctt aagagttgaa aaaacggata aaaagttagt aaactaaatg
     5221 ctactggcca ctgatagttc aaggtggttg ctctccaacc ggtttccccc attactgtca
     5281 atggtattaa acagtatata gagcaaacca ataataaatt tttaacattt attttctcaa
     5341 aaacatactt g
//
LOCUS       DAYYCY010000157         5303 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000157 DAYYCY010000000
VERSION     DAYYCY010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.158"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            30..371
                     /locus_tag="ACHAZB_002360"
     CDS             30..371
                     /locus_tag="ACHAZB_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFM8614984.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            680..1723
                     /gene="pheS"
                     /locus_tag="ACHAZB_002361"
     CDS             680..1723
                     /gene="pheS"
                     /locus_tag="ACHAZB_002361"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFM8614985.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            1728..4148
                     /gene="pheT"
                     /locus_tag="ACHAZB_002362"
     CDS             1728..4148
                     /gene="pheT"
                     /locus_tag="ACHAZB_002362"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFM8614986.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(4318..>5303)
                     /gene="trpS"
                     /locus_tag="ACHAZB_002363"
     CDS             complement(4318..>5303)
                     /gene="trpS"
                     /locus_tag="ACHAZB_002363"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFM8614987.1"
                     /translation="RPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIADMQALTDNAK
                     NPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVSVGRVRRNPT
                     VKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQTQEIVQSFN
                     HTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQKKVMSMYTD
                     PNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIKRYLIDVLEA
                     EFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINYFH"
BASE COUNT         1746 a          941 c         1159 g         1457 t
ORIGIN      
        1 atgctatact ctgggaagag gtgagtatga tggagctaac acagacagaa ttcgttttat
       61 gccctaaatt tgaaaaaaca ttctctattt taggaaaaaa atggaacgga ttgattatcg
      121 atgtactact cgaaaacggt ccgcaacgct ttgttgagct tgcggcaaaa atcccgatgg
      181 tcagtgatcg agtattagtc gaacgcttga aggaattaga aaaagaagga atagtcactc
      241 gaaactttgc ctgtagcgaa aagagccgtt cagaatatct cttgactgaa aaaggagcag
      301 acttgcaaaa agcgatgcgt caaatacaag tttggagcga aaaatggatc acagaggaag
      361 aatgcggttg attttgtaaa aaaaatcttg taaactatag tccatacgaa taagtaaaga
      421 acgatgacga aaaagagtag tttggctaaa ccagttcagg gagatctgtc gcagactgaa
      481 agcagatcct gtgtttgaaa aatgaagttc actttccgag ttcgtctgat aaaatcagac
      541 cggcaattgt cgttaacgaa gtaagagtgc ggtgtaagtc atttatccgc aaattaggat
      601 ggtaacgcga agacctcgtt ccttaacagg aatgaggtct ttttgttttg tatttggaat
      661 ttaaaggagg aacaagcaca tgtctctaca agcacaatta gagagcttga aagaagcaac
      721 gctcgtcaaa atcacagcgg cacaagattt aaatgaatta aatcaaatac gtgttgaaac
      781 gttaggaaaa aaaggaccga tcacagaagt attacgtggg atgaaagatc taagtccaga
      841 agagcgacca aaagtcggaa gtttcgcaaa tgagatccga gatcttttga cagaagagat
      901 cgagcgaaaa aaacaagtat tagaagaaga agcgttaaat gcagctttgg caaaagaaac
      961 aatagacgtg acactacctg gtagaaaagt aaaacatggt acacgtcata tcgtgacaca
     1021 aatcatggaa gaaattgaag atatttttgt gggtatgggc tatcagatcg tagaaggata
     1081 tgaagtagaa tctgatcatt acaatttcga acggatgaat ctgccaaaag atcatccagc
     1141 aagggacatg caagatactt tctatatttc agacgagata ttgattcgga cacatacttc
     1201 accagtccaa gcacggacaa tggaaaaaca tgatttctct aagggtgctc tacgaatgat
     1261 ctcgccaggg aaagtcttcc gcagagatac agatgatgcg acccacagtc atcagttcca
     1321 tcaaattgaa gggctagttg ttgataaaaa catcacaatg ggcgacctta aaggaacatt
     1381 agaagtcgta atgaaaaaaa tgtttgggga agatcgtgaa attcgtttgc gtccaagtta
     1441 tttcccattt acggaaccat cagtagaagt agatgtcagc tgtttcaaat gtggtggtgc
     1501 cggttgtaac gtatgtaaat acactggatg gatcgaaatt ttaggagctg gcatggtaca
     1561 cccgaatgtg ttgaagatgt caggaatcga tccagaagaa tattcaggct ttgctttcgg
     1621 ccttggacca gaccgcgtag ctatgttgcg gtatggagtg aatgatatcc gtaatttcta
     1681 tcaaaatgac ttacgtttct taagtcagtt caagggggag taagaaaatg ttagtttcat
     1741 ataaatggct caatcgttat gtagatttat cgaatgtaac accaaaagaa ttagcggata
     1801 agatgtctgt gacaggaatc gaagtagagg gagtaacggt tccagaagaa ggcttgaaaa
     1861 aaatcgttgt cggtgaagtc aaggaatgcg taccacatcc agattctgat cacctttcta
     1921 tttgtcaagt cgatattggc gaagacgaac tgtctcaaat cgtttgtgga gcgcctaatg
     1981 tgaaggttgg aatcaaagtg attgttgcac ttcctaactc acgtatcgct ggtaatgtaa
     2041 aaatcaaaaa aggaaaaatg cgtggccaag tatcaaatgg catgatttgc tcattacaag
     2101 aaattggcta ttcagatagt gttataccga aagaatacgc tgaaggtatc tattatatgc
     2161 cacaagaagc tgtaaatggc gatccagttt ttccttattt ggatatggat gattcgatta
     2221 ttgaattatc tatcacaccg aatcgtgcgg atgcattgtc aatgcgtggc gtagcttatg
     2281 aagttggggc aatttatcgt cagactccta cattcgatga cgaaaagctg gtagaatcct
     2341 cacgtcctgc tagcgataaa attcaagtgg aagttgaaga taaagaagca gttcctgctt
     2401 atcaaatcag aatcattgaa ggagtaagaa tccagccaag tcctcaatgg ctgcaaaatc
     2461 ttttgatgaa cgaaggcatt cgtccaatca acaatgtagt agatgtgacg aactatattt
     2521 tgcttttatt cggacaacca ctacatgcat ttgactacga caaattgaaa tcaaaagaga
     2581 tctttgtccg ccgtgcaaaa gaaaatgaaa cattagtaac actagatgga gaatctcggg
     2641 aattgacaac tgacaatctc gtcattacta acggcaaaga accagttgct ttagctggtg
     2701 tcatgggagg gttagactca gagatcacga atgaaacaac aacagttgca ctagaatctg
     2761 ctttatttga tccaatttcg attcgtcgta cagcaaaaca atttaatttg cgtagtgaat
     2821 cctctgcacg ttttgaaaaa ggaatcaacc atgcgactgt tggagaagcc agtgaggttg
     2881 ctgcagcaat gatcgtttct cttgcgggtg gggaagttct acaaggcgcg gtcaaaggct
     2941 cagagatcaa agcagaagac gtatcagtga agatttcact tgaacgcatc aatcaatatt
     3001 taggaacatc tcttgtcgtt aaagaagtaa atgagatttt tgaagcactt ggatttagct
     3061 atgaagaaaa ggagtatgtt tattccgtag tagtaccacc aagacgctgg gatatccaaa
     3121 tcgaagcaga tattatcgaa gaagttgctc gtatttatgg atacgaccag ttgccatcta
     3181 cacttccaag tggagaaacg gttgctggaa gcttgtcaaa agaacaagaa actactcgta
     3241 aaattcgtac aatctttgaa ggaagcggat tgagcgaagc aatcagttat gcattgacaa
     3301 cagaagaaaa atcaagacag tttgttataa atgaaagtaa tattacacgt ttggactggc
     3361 caatgagcga agaacgttct gtattacgaa tgaatttgat cagcggacta ttagatgata
     3421 tcagttataa cactgcccga aaaaatacag agattgcttt atatgaaatt ggtcgggtgt
     3481 tcttccaaga aaatgatcca cgtacacatc tgccgcaaga agtaaaacat gcagcaatcg
     3541 ctatttctgg tatttgggaa gaaaaagatt ggcagacgaa agctcaaaat gtagactttt
     3601 tcaccatcaa gggtctttta gaaaacttat ttgaacaact aggcatcact gaagatgtcc
     3661 attatcaaac agtgacagaa atgaaggaaa tgcatcctgg aagaactgct gcgatttacc
     3721 ttggcgaaaa atttattggt tttgtcggtc aagtgcatcc aacgattaca aaaagttatg
     3781 atattccaga aacatatgtt gctgagtttg atttatctgc tatagtggaa gctgctcaaa
     3841 aagggatcgt tttcgaagca gtgacaaaat tcccagcagt cagccgggac atcgctttgc
     3901 ttgtaaaaga aactgtgaca aatcaggaat taacagaagt tatccgttct gctgccggaa
     3961 gatttttaac agatatccaa ttatttgatg tctatcaagg agaaaacatt gaaaaaggac
     4021 ataaatccat ggcgtatagc ctgacattcg tcaacccaga agcaacattg acagatgaag
     4081 aaatcaataa agcaatggaa aaagtcgaaa aagcgttggt agaaaaccta gaggcaagta
     4141 ttcgctaaga aggaaaatat agtaagtaac gatcaagtag cttttgaaga ccatacattt
     4201 gaaaaaaaga tgtgacacaa cttcgtcaca tctctttttt tgattttaaa aaaggaatag
     4261 cacagagacg agataaaaaa acaggccttt acagcctgct tttttagagt aaatgaatta
     4321 atggaagtaa ttgattccca ttgctgattt tacttcagaa agtgtttgtg cagctacttt
     4381 agcagcttct tcactacctt tacgtaagat ttccataaca gctgaaggat ctttttccaa
     4441 ttcttctcgg cgcgcacgga tcggtgcaaa ttcagcttcc aatacatcaa tgaggtatcg
     4501 tttgatcttt acatcaccaa gtccgccgcg gcggtaatga gctttcattt cttcaattgc
     4561 ttctttatct gttccgaata catccaagta ggtgaagacc atattccctt ctacttgtcc
     4621 aggatcttgt acatggatgt ggtttggatc agtatacata ctcatgactt ttttctgtaa
     4681 gacatcggca ggatcggaga tataaatacc gtttcctaac gatttactca ttttgccatt
     4741 tccatcgatt ccaggtaatc tacccattcc ttttggtggg aatacgcctt ttggttccac
     4801 caagacttca ccatatgtat gattgaagct ttgtacgatt tcctgtgtct gctctagcat
     4861 tggtttttga tcctcaccaa caggtacaag attcgctttg aatgcagtga tatctgctgc
     4921 ttgagaaaca ggatagataa agaatcctgt aggcacgctt tcaccgaatt ttttttgttc
     4981 gatttctgtt ttgacagtcg gattgcgccg aacgcgtcct acgctgacta agttcaaata
     5041 atacatggtt aattcagcta attccggaat ttgtgattgg ataaataaag tagattttgc
     5101 aggatctaat cccacggcta aatagtctaa agcaacttgc aagacatttg atgaaacctt
     5161 ctcaggattt ttagcattat ctgtcagtgc ttgcatgtca gcaatcatga caaacagttg
     5221 gttgtcttca tctgcctgca tagccactcg ggtttttaga gaaccgacgt aatgccctaa
     5281 atgcagcttc ccagttggtc gat
//
LOCUS       DAYYCY010000158         5279 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000158 DAYYCY010000000
VERSION     DAYYCY010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5279
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.159"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(104..1909)
                     /gene="glmS"
                     /locus_tag="ACHAZB_002364"
     CDS             complement(104..1909)
                     /gene="glmS"
                     /locus_tag="ACHAZB_002364"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFM8614988.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            2318..2608
                     /locus_tag="ACHAZB_002365"
     CDS             2318..2608
                     /locus_tag="ACHAZB_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8614989.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            2621..3646
                     /gene="sppA"
                     /locus_tag="ACHAZB_002366"
     CDS             2621..3646
                     /gene="sppA"
                     /locus_tag="ACHAZB_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFM8614990.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            3649..4308
                     /locus_tag="ACHAZB_002367"
     CDS             3649..4308
                     /locus_tag="ACHAZB_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFM8614991.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            4465..>5279
                     /locus_tag="ACHAZB_002368"
     CDS             4465..>5279
                     /locus_tag="ACHAZB_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8614992.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRW"
BASE COUNT         1635 a          964 c         1109 g         1571 t
ORIGIN      
        1 atgtttttta atacctaaaa tgaaaatggg gctgaagaat caattctgat tcatcagtcc
       61 cataaattat agagcctgtc tgacactggt ccttgttctt ttattattct actgtcactg
      121 atttcgctaa attacgtggt ttgtcaacgt cgaatccccg taatagcgtt gcgtaatatg
      181 caagtaattg agtcggtacg actgtggtta gagtagttag ataagggtgg acagttggta
      241 gcaccagctg gtcactttcc ttttctaatc cctctgagac aataactagt gttttagcgc
      301 cacggctttc gacttctttc aggtttcctc gagtatgcgc accagtcact tcttcactga
      361 tgattccgat cactggtgtt ccttcttcga tcaaagcaat cgttccatgt ttcaattcgc
      421 cagcggcaaa accttctgcc tggatataac tgatttcttt caatttcaaa gcagcttcaa
      481 gagaaacgtt gtagtcagca ccacgtccaa tgtaaaaggc atttcttgta tcgctgagat
      541 atacttctgc caactcttct aaataatctt tttgggcgat gaccgattcc atcgcattag
      601 caactaaact caattcatga gtgatatcga atacaagaga agtttcaaat tcttttttat
      661 caccgatagc tttggctaat aaagttagta cagctatttg tgcagtataa gctttcgttg
      721 atgctactgc gatttcgggt ccagcatgca atagcagtgt aaaggatgct tcccgcgaaa
      781 gagtagaacc agcaacattt gtaatcgtaa gtgaagggta gccgagacgg ttgattttta
      841 ccagcacttg gcggctgtct gctgtttcgc cactttgact caagaaaata aagaatggtt
      901 tttttgaaag aagaggtgtg ttatagccaa attcactggc taaatgaacc tcaacaggaa
      961 tctgtgtcaa tttttccaaa gtctgtttac ctgctaaccc cgcatggtaa ctagttccgc
     1021 aagcaacgat atagattcga tcgctatcag acaatgtatc gaccagcttt tgatcaagag
     1081 agacacgatt gtcttctgtt acgtattttt gagcgatacg acgcatgata ataggctgtt
     1141 cgtcgatttc tttcagcata taaaaaggat aggtaccttt ctcgatatct gaagcatcta
     1201 cttgtgcttc gaatgaagaa cgttctacga tgtcacctgc aaaattttga ataataactt
     1261 tttctgatgt gacggtaacc atctcttcat cttcaatctc aacgaattcc ttcgtatgag
     1321 caagcatagc cattgcatcg ctggcaatca cattgaatcc atcgccgagt ccgattaata
     1381 gtggactttt atttttagcg acgtaaataa catcaggtgc tgtccgatca atcaaagcga
     1441 aagcgtaaga acctttgatg acccgcaaag cttttaaaaa cgcctcttgc gttgtagtat
     1501 ccttcgcgaa agtttcaatt agatgagcga taatctcagt atcggtctca ccaataaagt
     1561 ggtcatttgc taaataagca attttaagtt cttcaaagtt ttcgattacg ccgttgtgaa
     1621 cgagcacgaa gcgtccactt tgggaagtat gtggatgggc gttttcttca gaaggttgcc
     1681 cgtgagttgc ccatcgtgta tgaccaatac ctatatttcc tgtagtatca atatttattt
     1741 tgtttttcaa attttgaatt ctgccttgtg ctttgaccag ataatcagtt gtgccatctg
     1801 agacaaagat accagctgaa tcataaccac ggtattctaa tttttctaga ccgtcaatca
     1861 atcctggtgt tacatttttt aatccaatca ttccaacaat tccgcacata aaaaacatcc
     1921 tactttctat aatgaattcg tatgaatgct tccagctgca ggaaaatcct tttgttttat
     1981 ctttgcagac attttttgtg gattttctgt agagtgcagt ccctagggcc atccgccgaa
     2041 tctttcgata aaccctctcc tcgtcatctg tttacacaga cctggcgcta tcctaaaagg
     2101 tataactatc ctcctttttg ttttttatct ggaaacattt aaaatcataa ccttttgcga
     2161 atgttcttgt caacaaaaaa agaataaaaa ataaattggt atatatcaaa ttctttgtca
     2221 aatgtccttt ttatgtcaaa aaataatggg tttttatcta tcatcttttt caggagttcg
     2281 gtacaataga aagtaatcgg atagaggagg agtaaaaatg gattcaaaaa ttgagatcat
     2341 gacactggga atgttaaaaa aacaattgtc caaattcgaa gcatcagctg gtgtatcaga
     2401 tgatacgaaa atatttttag atactggctg ggatagcatc caagaaatcg cacctgatgc
     2461 cttggaagtt gctcaagctc gtgagtttac ggtagaagat gaattaacaa aagagtcgtt
     2521 ttccgggtat gcgcgggaag aaaaagcaga gcggttcgac acttctgagc agtcagaaac
     2581 tgtgatcgtc ataaaaaact tgtattagaa aggagaaaga atgaataaaa gacgttgggc
     2641 agcagttctg attgctgcag gattatttgt ggtttcgtta gtttcagcta gtttgactaa
     2701 gcctcaggaa aacgatcaaa ttgagggatt gaacagttgg ttatatgggg atgaagaact
     2761 agctccaaat gtgttggaag agggagacag tacgcaaaaa atcgtaaaat tatcagtaga
     2821 tggaacgatt gctgcgggag ggtcttcagg tttatttgct tctgaaggtt acaatcataa
     2881 aaattttatg gaacaactga aggcgattga agaagatcca acagtcaaag gtatcttttt
     2941 agaagtaaat tctcctggtg gcggtgtcta cgaaagtgcg gaaatcgcta agaaattgga
     3001 tacgattcgt aaagaacatg atatcccaat gtatgtcagc atgaaaaaca tggcagctag
     3061 cggtggctac tatatttctg ctcaagcaga taaaatcttt gcaacagaag aaacagtgac
     3121 cggctcaatt ggtgtgatca tgtcaggatt gaattattca ggacttctag aaaaactggg
     3181 tgtggaagat acaacagtta aaagcggagc gttgaaagat atgggttcag gaacgcgacc
     3241 agagacaaaa gaagaagaag cggtgcttca agcatatatc gacagcgcat atagtcgttt
     3301 tgtagcaatc gtcagcgagg gacggaataa atctgaagaa gaagtcaaaa aaattgctga
     3361 tggaaggatt tatgatggtg tccaagcaaa agaagtaggt ttagtcgatg aattgggatt
     3421 tcctgaagat gctttaaaag ctatacgaaa ggaacaacag cttgaagatg cggaactagt
     3481 agaatattct tccacgccat ctgggtttgc tagtacatgg ttaggaagta aactagcaga
     3541 attccaggga ttgaagcctt ctgagactag tcagatctta tccgttcttg agaatctagg
     3601 gacagcggaa tcaccaaaag caatgtatta ttacggaggt gagtaagcat gtcagagctt
     3661 tcaaatgaaa ataaggaaca agaaaaagat aagcaacagc agcctgctgt aggacctgac
     3721 tccaactttt cccaaaaggt actgagctct tttgaaagag agcctttgac gcccgaagaa
     3781 atcaaaaaga aacaacgtaa atggaaaaag tatacggaca caagaaatga accgccaaaa
     3841 gcaaatgatt ttcctaagta tttctatgca ggttttttta tccgaatgtt tgccttcttg
     3901 gtcgatcttt tgtgtatcgg agctattacg cgtttaacac ttggtattgc caaaaatcta
     3961 ggttggctta atttttcaga ttcttatctg agtctctacg gattcacagc cttacttatt
     4021 tatctagcgt attttatttt attgacgaaa ttcaatcatg gccaaacgat tgggaaaatg
     4081 atctttggta ttcgagtcat cagctttgat gaagaagaac ttagctggtc aaccgtttta
     4141 gttagagaag gtgcttgccg gttcattcta aaatttcctt tgctgatgtt agggtatctt
     4201 ccagcagtct ttaatcagaa aaagcaacat ctgggcgatt ttttcactaa tacaagtgtt
     4261 gttacgatca atctgatcaa agcgttcaac caggaagtca acagctgatg agcaataaaa
     4321 agcagtaaca gttgaaagca ccttctaaaa gtcagatcta aaatctagct tttagagggt
     4381 gcttcagtta tttttaattg tcatgctttt tttgttttgc tactattgaa catgagctga
     4441 atatgagcaa gaggaggaca ctgcgtgaag ccgattgatt ttccagaaaa atatgagaat
     4501 ttaatgcgag tggcacaaca agcactagca aaccagcaat atcagcaggc aaaagaatta
     4561 tttcaacgag cttacgagtt gaaagaaagc tttgaggcta atagcctgct tgttttttgt
     4621 ctttacgagt tggacgagaa aaaagaagca ttgaagcaag cactcttgca tgaaaaacag
     4681 tatcttcaaa atgaggagtt tgctgaattt tattttgact tgttaatcag tacacaagat
     4741 tttttatatg ctagaaaatt gatagcaagt acagattttt atgaagcttt cgagcagcgg
     4801 gtcattgaaa aaatccaatt tgcagaagaa ttatccggac aaatggagag gcagaagatc
     4861 aaggcactcc acaataaatc ggaggaactt ccttcgttgg aacctgcacg tcagcttgcg
     4921 tctattgatg agatcgaaca gcttccttat catgagttta tccaaacagc gagtaaactt
     4981 attgtatcgc cagaggtcca tattttagca agggcaaaat tactggagac acttaggcaa
     5041 ttgaacgaat gccatcctgt tttctatttg acgattgaag aaaagttagt caaagtcata
     5101 ccaaaagatc tgccaaagcc gcagcagcag gcaagttacc agcaattatg tgtattttca
     5161 gatcgttacg ggaatgaaga tgcgctgctt agttccgtgt taaaagaaga attcacgttg
     5221 caatctgcaa ttgtttatcc tgtctatgat acatacatcg aagacccgaa gcgctggtt
//
LOCUS       DAYYCY010000159         5269 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000159 DAYYCY010000000
VERSION     DAYYCY010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5269
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.160"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(61..427)
                     /gene="ssrA"
                     /locus_tag="ACHAZB_002369"
     tmRNA           complement(61..427)
                     /gene="ssrA"
                     /locus_tag="ACHAZB_002369"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(598..1371)
                     /locus_tag="ACHAZB_002370"
     CDS             complement(598..1371)
                     /locus_tag="ACHAZB_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8614993.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            1515..1865
                     /locus_tag="ACHAZB_002371"
     CDS             1515..1865
                     /locus_tag="ACHAZB_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFM8614994.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            1846..2562
                     /locus_tag="ACHAZB_002372"
     CDS             1846..2562
                     /locus_tag="ACHAZB_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFM8614995.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            2562..4010
                     /locus_tag="ACHAZB_002373"
     CDS             2562..4010
                     /locus_tag="ACHAZB_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8614996.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4080..4589)
                     /locus_tag="ACHAZB_002374"
     CDS             complement(4080..4589)
                     /locus_tag="ACHAZB_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8614997.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(4579..>5269)
                     /locus_tag="ACHAZB_002375"
     CDS             complement(4579..>5269)
                     /locus_tag="ACHAZB_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8614998.1"
                     /translation="YYKKQTILYCLFSSFSAFLLIACLTLYHLQSALANQVQDRLSFI
                     GASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNKQELINWRLTGLS
                     KTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVLKTTELPDIRDIL
                     TSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLILSLFPFTFFIHH
                     LQSRRPGRNET"
BASE COUNT         1780 a          927 c         1053 g         1509 t
ORIGIN      
        1 ataaaaaaac gttgatttaa caacgttttt catacatgtt tatctgtctc aaccatacat
       61 atatggagac ggcgggagtc gaacccgcgt ccaaacacat cgccacttaa aaatctacgt
      121 tcatagtcaa ctcatttaag gattcacttt acagcttgcc gagtgacagg cattctgtat
      181 cgctagtctg atgttctctt tttcaactta cagacggaaa gttgaaacgt atcccactaa
      241 agttaggacc cttactcgag cacatgggcg atgccgagag gatctacgct aactgttttt
      301 aggcagctaa agcgtaagag ttgttttcgt ttttagcagt tatatttaac tgtaacgttt
      361 ttacgtagac gtaacctacg aaacgcaatt caagctcgac tgtgcctgtc gaatccgtaa
      421 cgtccccgtt agctaacaag ggttacgtgt atttttcata caaaattagt atagcatata
      481 ttgggataaa ttcaataaaa aagcgaatca tatcccatta tttatataat aactgcccct
      541 ttcttaagaa aaaataagcg attttcttaa gaaaagaggt agcttcaaaa aaagatctta
      601 aatcagatca aaatacttca atgcgtggac aattccgcct tctgtatttt tctttgtgat
      661 aaaggtagaa agttctttta atccgtcata ggcgttgccc atagcgattt tgttgtcgca
      721 agcttccaac aaggcaaaat cattcggacc atcgccaaat ccaaaagtag gaactttttc
      781 cagctgaagt tctttttgca gacgtttgac acctgcccct tttgagattc ctttcttcac
      841 tgtatcgatc gagtaaggac cattccgata aaaagttagc tcaggaaaat gttcgtgata
      901 gtattcgtca cctgattgag ataagatcag caacatattt actgtgtttt ctttaaagcc
      961 taaaggatca atttccggaa catctgaatg aatgaagtcg taagcattga tcattgtttg
     1021 gctatgaccg gtacaccaga ttttctgatc gttatagtaa gaaagttcat gaccgttcaa
     1081 ctgtacatga tcatacattt tttgacattc ttctgaagaa aatgtttgag aatagatttc
     1141 ttcgccgtct actcgaatga aagaaccatt catggtgacc gcagaagtaa tgcccgcatc
     1201 ttccatgata gagtggattt ctgcttctgt acgtcctgta gcgatgacgg ggagaacatt
     1261 gttttctttt aatgcgttca tagcttttgc cacttcgggt gtgatttttg atttcgcatc
     1321 tagtaatgtt ccatcaagat caaagaaagt gattgcacga taatttgaca caaataaaac
     1381 tcctttttta ttggaataag tttatcttaa caaagaatga acaaaagaca aacctttcgt
     1441 gaagaattcc tgaacatatt ggttcagtca acaatttaga gtatactata agaagagaat
     1501 ttaggaggga aagaatgatt tttttcagta ttggtatctt attcttaata attggcagtc
     1561 tttttcggat tctgccctca aaaggaaatc tgccttttta tgggtatcat tcgcctcttg
     1621 ccgcaaaaac agacgctcat tggcggctgg ctcaaaagac aagcggaaac tggttttttc
     1681 ttatgggctt actgatggca ttgatcggct attatttaaa gacatctggg catacgaatt
     1741 attttctgat tgaaatgctt ctgctagtat ttccaattat gcctattttt attatgacag
     1801 aaaagaaatt acagaaatat gacctggaaa ctggagggaa cgataatgaa tattttaatg
     1861 attgaagata atcaatctgt ttctgaaatg atgcagatgt ttttcttaaa tgaaggttgg
     1921 gaagcaactt tcaagtatga tggaaaagaa gggctcgatg cctttttaga aaacccacaa
     1981 cattgggata tgattacact agacctgaat ttgcccacac tggacggtat gctagtagga
     2041 agagagatcc gcaaagtttc aagtactgtg ccgattatca tgctgacagc tagagattct
     2101 gaaagcgatc aggtgatcgg cttagagatg ggggcagatg attatgtcac aaaacctttc
     2161 agtcctctga cgcttattgc tcgaatgaaa gctttgcatc gccgtagtga gttggcagaa
     2221 gcaagagaag aagcagcagt ttcagatgat cattttgata tcgagactag tcatttcaag
     2281 atgaatacga aaacacgaga agcgtatctg gacaaccggc caattgaagg actgacgcct
     2341 aaagagttcg atctattgtt tactttagca aaaaaaccac gtcaagtatt ctctcgggag
     2401 cagttgcttg aattagtctg ggattatcag tactttggtg atgaacggac agtggacgcc
     2461 catatcaaga aattacgaca aaaaatcgaa aaggtcggac cacaagtcat ccaaactgtt
     2521 tggggagtcg gctacaaatt cgatgattct ggtgttgcct aatgcgttat ttgtatcagc
     2581 aactcttagc tttttgcgga atggtcgcgt tgatcttatt gatcgttgga gtttctttta
     2641 cacaactgac aaaacaaacg attgaagaaa acaattacca gcaactgttt ggatatgctg
     2701 aatcagttga aaagacaacg caaacctatg ccgatacgct cccgcagttt agtcaagatc
     2761 aggcgtttca aaatgcattg ctgctgacag agcaagtact gacagaacaa aatgtgaact
     2821 ttatatttat cgataaaaac gaacgtgtca tttatccgac taatacagct ttactgaatt
     2881 ttaccttaac agctgatcaa tggaaaaatc tgaggaatgg acgtcaagaa aaattcacat
     2941 cgaataaaaa tattttagga caagcacagg cgacctctta cgcacttgtg ccatttaatt
     3001 tgaatcatga attctatgga gcacttgtag ttagtcaacc tgcgcgaaat atcgataaca
     3061 gcgttcgtcc aattacactg aacctgttca aaggatttat tttttccagt atcgttgcag
     3121 ttattgccag ctatgctttt gcagcattcc aagttaagcg tatcaatcgg ttaaggaatg
     3181 cgacgaaaga agtgacgaat ggtaattttg atgtacagct ccccgtacat gacaaagatg
     3241 aatttgatga gttagcagat gatttcaata aaatgacgaa ttcattgaaa gaatctcaag
     3301 cagaaattga agaacaagaa aatcgtcgaa gacaatttat ggcagatgct tctcacgaga
     3361 tgagaacacc tcttacgaca atcaacggac ttctggaagg gttagaatac aatgcaatcc
     3421 ctgaaaatca gcgagaaaac gcgattaaat tgatgaaaaa tgaaacagag cgtctgatcc
     3481 gtttggtgaa tgaaaacttg gattatgaaa aaatccgtac gaatcaaatc agtatggtca
     3541 ttaaaaaatt caatggaaca gaaacattga aaaatattgt tgcacagtta gaagctaaag
     3601 cagaagcagc aggagataca ttgacattga aagccgttga tgatattgat gtttacgctg
     3661 attatgaccg atttgtccaa atcatggtca atattattca aaacgcgata cagtttactg
     3721 aaaacggcca aatcacgatt actttagaaa aaggctatct tgaaacgatc atcacgattg
     3781 aagatacggg aattggaatg tctgaacaac aaatgaaaag tatctgggat cgttattata
     3841 aagtagatcc ttcaagaaaa aatacaaaat atggtgaatc aggattaggt ttaccaatcg
     3901 tgcagcaact tgtacgcttg cataaaggaa aattagaagt agaaagcgaa ctaggaaaag
     3961 gaacaaaatt cgctgttcgt ttgcctgacg tagaaatcaa aaaagagtaa aaaacaagcg
     4021 gtataaaaga tcatgaatcg atcttctata ccgcttgttt ttactataaa atgattgatt
     4081 tagcccgcgg gctctaaacg atcaatataa ggactttgag caatagtagc atcatgagta
     4141 aagagtaaga taccttgatt ttcttcatga gctaaataag aaagcaagtg gagtagctct
     4201 tggatctcca atacactaag ttcatcaaat gtatcttcaa taacaatatt gtttcgcttg
     4261 gaaagcaagg catgaagtaa ttgcagcttt actttttcaa aagacgtcaa ctcagctgcc
     4321 gcacaattaa gcaaggaagg agagatatca agtttttctt gataggccaa acgatcttgt
     4381 ttgatttctt tttccggtac atccaacagg acatggtctt taagattcag ataaggaaac
     4441 atcggccaat ttttttggat gatcgcaaaa ttatccagtt ttgcaacaag aacctctttt
     4501 ttcaagtgct ccaatatttt atgctgtttt tcgagaggac acaaaataac ggtcaagtgt
     4561 tcagcatgaa tcaaatattc aggtttcatt acgtccaggc ctcctacttt gcaaatgatg
     4621 gataaaaaaa gtgaaaggga atagactaag aatgagacag catccactga gcccaataag
     4681 agtttgggca aatcccttaa gtatatctgt gaaaaaaaga tcattttgga aatcgatggt
     4741 aaaaaaagtc tgttgatcca tcggaattgt aagtccgctt accgaagtca agatatcacg
     4801 gatatctggc agttcagtag tctttaagac aagaaaattg atcctttgaa gaagcgcttc
     4861 ataaactgac tggaagctta cgatccacaa taaaaccaag atacaacaaa gaaagagagg
     4921 aaagaacagc tgccagaaaa taaaaaggaa taatttggtt ttagaaagac cagtcaggcg
     4981 ccaattgatc aattcctgct tgttttgcct gactgaatag taaaagaaaa agataaaaag
     5041 gaaggcaaat agaaaaaata aactgaatat gccttttata taaaatcggc tagctaattc
     5101 taaagccgga agaagctctg tagatgcaga agcgccaata aatgaaagcc gatcttggac
     5161 ttgattggct aaagccgatt gcaaatggta aagagtcagg catgctatca ataaaaaagc
     5221 agaaaagctt gaaaataaac aatagagtat tgtctgcttt ttgtaataa
//
LOCUS       DAYYCY010000160         5154 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000160 DAYYCY010000000
VERSION     DAYYCY010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5154
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.161"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..649
                     /locus_tag="ACHAZB_002376"
     CDS             <1..649
                     /locus_tag="ACHAZB_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8614999.1"
                     /translation="DTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGEIYQAEGLS
                     AVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTAAELQKQMR
                     YQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSIQFPQLITM
                     IHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVAMEKAN"
     gene            708..1148
                     /locus_tag="ACHAZB_002377"
     CDS             708..1148
                     /locus_tag="ACHAZB_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8615000.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1160..1828
                     /locus_tag="ACHAZB_002378"
     CDS             1160..1828
                     /locus_tag="ACHAZB_002378"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFM8615001.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1892..2515
                     /locus_tag="ACHAZB_002379"
     CDS             1892..2515
                     /locus_tag="ACHAZB_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HFM8615002.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2571..2957
                     /locus_tag="ACHAZB_002380"
     CDS             2571..2957
                     /locus_tag="ACHAZB_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HFM8615003.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3170..4561
                     /locus_tag="ACHAZB_002381"
     CDS             3170..4561
                     /locus_tag="ACHAZB_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HFM8615004.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
BASE COUNT         1708 a          861 c         1084 g         1501 t
ORIGIN      
        1 agatactctg attgagcgat ctacatttct atatgttcaa ttgcaggaaa ataaaacatt
       61 gctgcatttt tcaccagagt tccatctaga ggggctaaca ttaggagaaa tctatcaagc
      121 agaagggctg tcggctgttt tgcacttttt taaggtagaa ttggaattac cagtaaaaga
      181 ttatatcgag ctttctcttg aatcttggga tcgagcagtg gttgaactgt ttccagaagg
      241 tttaatgatc gatacaaacg atgggaagat ccatctgact gcagcagaac tgcaaaaaca
      301 aatgcgttat caaatagatg acgaaaattc tttttctatt tttagacgtc agcaaaagat
      361 tctaaaaagc tttttaaaag tgatcagtaa aaaacggaat ctgatcaaaa cgacacagct
      421 gttgaaaaaa tatccaggtc tgcttcacac gtctattcaa tttcctcaat tgattacgat
      481 gatccatcgt tttatacgta tgcaacagct accatctaag aagctgtttc ttccattgac
      541 ggacacattt cgagtagtca acagagaaga taaaaagaaa gtgattgtga ttgactttac
      601 cagaaatcgg aatgtccttc accaagttgc aatggaaaag gccaattaag gctttttctt
      661 tttggatttt tttgtcataa taaatattag atgatggagg agataatatg atgcaaacat
      721 ggttagaaag gctaatacga tttcagcaga actatcacaa acttatgcag ggaagatatg
      781 cgaaatttga tcagttgaat cgcggtctat tgatcgctgc gcttgtcttt agtttgctaa
      841 gtcgttggtt gccctataga atcggacaat tgttgttcat cgtctttttc ggatggatga
      901 tatttcgttt cctatcaaaa aaaatctacc ccagactaaa tgagaatcaa cgttatttga
      961 aatatcttga tagagtcaaa gaaaaatatc atcagataaa aaacaaaaag caagtcaaaa
     1021 tggatcgaaa aagttatact ttttttgagt gcccgaattg tcatcaaaag cagcgtgcac
     1081 ctaaaggaaa aggacgaata cgtgtgacat gcaaaacatg tggtataaaa ttcgaaacga
     1141 atgtttaagg gagaaaagaa tgaaattagt attattacgt catggcgaaa gcgaagcaaa
     1201 ttttgaaaat tactggacag gatggctaga tgtcgcctta acagaaaaag gaaaagaaca
     1261 agcagcagtt gccggtcaaa aaatgcttga agccggactt caattcgatg ccgtttatac
     1321 ctctgttttg aaacgtgcga ttcttaccgg acaagtcgca ctagaagaaa tgggtcagtt
     1381 atggcttcct ataataaaaa catggcgtct aaatgaacgg cattatgggg atcttgtggg
     1441 taaaaacaaa gataagatga aagaaatata cggaaaagat caagtaaaaa catggcgcag
     1501 aggatatacg gaagttccgc cgttaaccgc agaaaataga tttgaccgca gatatgatca
     1561 cttggatccg catttgatcc catacggaga aagtctggaa atgacagtga atcggatcat
     1621 tcccttatgg caagatcatc tagcaccaaa actaaaagat cggcaagatt tactagttgt
     1681 gggtcatggt aatagcatcc gagcactgac aaaatattta gaagatgttc ctgaagatca
     1741 aatggatacg atcgatatcc caaatgcaca accaatccag tatacattag cagatgattt
     1801 gacaattctg gacaaaaaaa tactttaaag aacaccttta tctaaaagtg agagatagaa
     1861 aaaagaagca aaggaatgga acaaaaggaa catgaaaaat tatatatggg actttgatgg
     1921 tacattgttt gatacgtatc ccgcaatggt agatggtgca tggcaagcac taaaagattt
     1981 tggtatcagt atggacaaaa aagaaatcta cttcaaaatg aaaaagtatt ctactagtta
     2041 tttgatcaat gaatcaaact tgaatgccag agagtttaat gaattatttc accgatatga
     2101 aaaagaaagt acggaagttt cccgtccatt tccagaaacg aaacaagtac tggaaatgct
     2161 aaaagacaac ggcggtcgtc atttcatcct gacccaccgt ttgacagaat caacatgggg
     2221 cctgttaaaa gaacatcgat tggcacatct gattgaggaa gtagtaggga tcgatcagga
     2281 ctttcctcgt aaacctgatc cagcatcttt aaattatttg atcgatactt ttcatttaga
     2341 aagaacagac acgatgatga tcggcgatcg tcgtttagat atcgaagcag ggaaaaatgc
     2401 aggagtcgct acttgtcttt acgacatcga tcattttctg ggtgagatcc cggcagatta
     2461 tgtggtagga aatctgaatg aaatccttac cttgcagagc atcattaaaa agtagtatcc
     2521 cctttcaaag gagatatgtt ataattttca taaaggagtg ataaaattat atggaaatca
     2581 gaagacaaaa agaagttgta gaagcatttc attatgatat gagattgccg aaccaagaag
     2641 tagaaacaga tttgaaagta ggcttctcgc ctttaaaaga aacgatcgaa aactatccga
     2701 aagaacatag catcgtagga gcacgcttag agttccgttt agtgtttgat acatatgttt
     2761 tatctggaag tgtgagtcag atcaatcata tcctcaatcg gaaaattgaa ggtcaaaatg
     2821 atgtcacaca agaagaagtg gacgagctag tagcaccgtt attcgatatc gtacgccgaa
     2881 tgtcttatga ggtgacagag atcgccttag atcagcctgg ggtaaaattg aattttcaat
     2941 cagaaaaaca agaatagaca gtaaaaacat acctgcgata gattaaagct atcacaggta
     3001 tgttttttta ttactaattg taagcaaaaa gaatgtcatt tcgataaaaa tcgtctaaaa
     3061 tataaaagtc actgaaaaaa attttttcag gaggatggtt gaagaatcat tctcgggaac
     3121 ctttacttcc ttcgatggac ttgtcattaa taatatgagg aggagaaaaa tgacaacact
     3181 catcacagtt ttagtggttc tagtttttgc ggcattatta tatgtgttct atcgtatgca
     3241 aaagcgccat acaaaatttt caacacgcgt ctttacagca ttaggagttg gtatcgtctt
     3301 tggcggtctg cttcaattga tctttggtgt agacgacaaa gtcgttactc aatcaatgga
     3361 atggatcggg attgtaggtc aaggatatgt atcattcttg caaatgctag ttatgccttt
     3421 agtgtttatt tcgatcattg gtgcattcac aaaaatgaaa gtttctgaaa agctagggaa
     3481 aattagtgcg actgtattga cttctttact tggaacaaca gctattgcag cattgatcgg
     3541 aattggttca acaatgctgt tcggactcca aggagctaaa ttcacacaag gagctgcaga
     3601 aacagcacga atctctgaac tggcaacacg tcaggaaaca gttgaaaact tatcgattcc
     3661 acagcagatt ttatccttta tccctaaaaa cgtttttgct gatttagcag gtagtcgtgc
     3721 aacaagtacg attggagtcg ttattttcgc ggcatttgtc ggaatcgctt atctaggcgt
     3781 gaaaagaaaa gcgccaaaag aagcagcatt cttttcagat ttagtcgaca gtctgtataa
     3841 aatcattatg cggatcgtga cgcttgtttt gcgtttgaca ccttatggag tatttgcttt
     3901 gatgacaaac gttttggcaa caagtgattt tgcagctatc gtcaatttag gtaaatttgt
     3961 cgttgcttct tacgtagcat tgcttgctgt tttgattgtc cactcactga ttttgattgg
     4021 tgcaaaagtc aatccaatca actatttcaa aaaagccttt ccggtattga gctttgcgtt
     4081 tacttcacgt tcaagtgctg gagctttgcc attgaatatc gagactcaga caaaagcttt
     4141 aggagtagat gatgcaacag ctaatttctc agcaagtttt ggtttgtcta ttggacaaaa
     4201 cggctgtgct ggcgtttatc cagctatgtt ggcaacgatc gtcgcaccag cagctggagt
     4261 gaatgtcttt agtttggaat ttattttgat gcttgtggca attgtaacaa tcagttcttt
     4321 tggtgtagct ggagtcggtg ggggcgcaac atttgcttca ctgatcgttt taggggcaat
     4381 gaacttgcca gtagcaatcg ttgggttagt tatctctgtt gaaccattaa tcgatatggc
     4441 acgtacagca gtaaacgtca gcggtagcat ggtagctggt atcgtgacaa gtgcacgcat
     4501 caaagattta gatcgcgatg tcatcaatga tgattctaaa gtgatcgatg tgaatgcata
     4561 gtcaaaaaga ggttgtgata gaagtggtca gctttaagaa ataagaagaa gtttcctaaa
     4621 aatagtttct caaatttttg tgaaatttcg gcttatttcc gagaagctac ttctgaaaca
     4681 ccgtgaataa gaggttgtga aaaagctgcc ctgcatcaag taataagaac agccaaacaa
     4741 aaatagttcc tcatatttta cgtttggttg agcttattac cgcagatgca agcttttgaa
     4801 caccgtttaa gaaagaggtc gtgagcctgc cgtttcactc tgagtcacaa agagcaattt
     4861 caaaaaatag cttctcatgt ttgttgaata ttgtgtcttg tgaccgagga gttgtcagga
     4921 gaacaccgtt taccagaggt tgtgacttgt ttttagtcac gacctctttt tttatttatt
     4981 ccatataaat acacattatt tattccgaaa agtgaaagcg caaataacaa aaacaaccaa
     5041 cttatttatt attaaagaaa agcgtattta agtaggacga ttctgttgag agctttcgta
     5101 gattttccca ttttcctatg gtaaactgac aaagttgaga atgttagaac agat
//
LOCUS       DAYYCY010000161         5089 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000161 DAYYCY010000000
VERSION     DAYYCY010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5089
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.162"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            120..1751
                     /locus_tag="ACHAZB_002382"
     CDS             120..1751
                     /locus_tag="ACHAZB_002382"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFM8615005.1"
                     /translation="MVLIKDWQDSIEGLNKMQVQLPYFNVEAVKQEGQKHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELESGVVVCETFDEEVVEKAYHDYNNDILEIVMGEDGEL
                     KKRLLAVTADSLYCNPKNITDYQRVVKYFEDPTLQFTTFTITEKGYSLKDSKGQFFSV
                     VEADIKSGPTNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFMIAE
                     AWQKKGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSESVKMDLLKAGFEDLDIIHTA
                     KNTNIAAFTNTEKVHYLVIEDSFPNGRPKLEAAGVIMTDRETVDKADIMKVTTCLNPL
                     HTALAITGCLLGYKSIQAEMQDDDLVGLIKGIGYVEGLPVVESPGVIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGKNFVPSPDPLLVELQEKFSVLKLGEQDPAKIHCAVQEILENKEIFGLDLYQ
                     VEIGKKIEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            1754..2833
                     /gene="uxuA"
                     /locus_tag="ACHAZB_002383"
     CDS             1754..2833
                     /gene="uxuA"
                     /locus_tag="ACHAZB_002383"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFM8615006.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKALKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYHLDDGSSTLAYISKNIPTDPEEIIRKVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEAKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLEWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            2907..4334
                     /gene="uxaC"
                     /locus_tag="ACHAZB_002384"
     CDS             2907..4334
                     /gene="uxaC"
                     /locus_tag="ACHAZB_002384"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFM8615007.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAEHQPIIDYHCHLDPACLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGAGNDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLKMMDVKAICTTDD
                     PTDDLRYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQAAGIPIKNFNGL
                     KQALSQRVDYFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIESRK"
     gene            4418..5065
                     /locus_tag="ACHAZB_002385"
     CDS             4418..5065
                     /locus_tag="ACHAZB_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFM8615008.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINAITAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVEIIKLFPGSAFGPSVVKTYQAPLPYVNIMP
                     TGGVSLDNLEEWFEAGVVAVGVGGNLLAPVEKGDFEEVTRIASKYIDKFYEIKNY"
BASE COUNT         1773 a          697 c         1070 g         1549 t
ORIGIN      
        1 aataattctg acatggtaga aaaaagaata tttttagttt tttcttattg acagcgatta
       61 cattcgattt tatactacaa ttgtaacgga aactaacata tcagtatgga ggggtaaata
      121 tggttttgat taaagactgg caagattcta tcgaaggatt gaacaaaatg caagttcaac
      181 taccgtattt taatgtcgaa gcggtcaaac aagaaggtca gaaacatcca gtgtggcttc
      241 attttggtgg aggaaatctt tatcgaggat ttcatgcaga aattgctcaa tgcttagcag
      301 atcaaaatga attagaatcc ggagtagttg tgtgtgagac atttgatgaa gaagtagtag
      361 aaaaagctta tcatgactac aacaatgata ttttggagat tgttatgggt gaagatggtg
      421 aactcaaaaa aagactttta gcagtaacag ctgatagttt atattgcaat ccaaagaata
      481 taacggacta tcaaagagtg gttaaatatt ttgaagatcc tacattacag tttacaacgt
      541 ttactattac tgaaaaagga tacagtttaa aagattctaa aggacaattt ttttcggtag
      601 tggaagcaga tataaaatct ggacctacca atcctcaaca tacgatgagt attgtgacat
      661 ccttgttgta tagcaggttt aatgctggag agttaccgat tgcgatggtt tctactgata
      721 atttctcaca gaatggtcaa cgttttcaag atagcatttt tatgattgca gaagcttggc
      781 aaaaaaaagg atacgtttct gaaaaattta ttgcttattt aaaggataaa gaaaaagtga
      841 cttttccttg gagtatgatt gatagaatta cgccaaatcc ttcggaaagt gtcaaaatgg
      901 atttattgaa ggcgggtttc gaagacttag atataattca tactgcgaaa aataccaata
      961 tcgctgcttt taccaatact gaaaaagtcc attatctagt cattgaagat agttttccaa
     1021 atggtcgacc taaactggaa gcggctggtg tgattatgac agatagagaa acagttgata
     1081 aagcagatat catgaaggtt acgacatgtt taaatccgct ccatacggca ttagcaataa
     1141 caggttgttt gttggggtat aaatcaattc aggcagaaat gcaagatgat gatttggttg
     1201 gcttaattaa agggataggt tatgtagaag gcttaccagt agttgaaagt ccaggcgtta
     1261 ttgatccaaa acaatttatt gatgaagtaa tcaataaacg tttgcctaat ccctatattc
     1321 cagatacacc gcaacgaatt gcaacagata cttctcaaaa agtagcgatt cgctatggtg
     1381 aaacaattaa aaagtatgtc gaaaaagacg gaaatgcaga aaatctaaca tttattccta
     1441 tcgctattgc tgcttggtta cgttatttat tggcagtcga tgatcaagga aaaaattttg
     1501 tgccaagtcc tgatccttta ttggtagagt tgcaggaaaa attctcagta ttgaaattag
     1561 gtgaacaaga tcctgcaaag attcattgtg cggtacagga aatcttagaa aataaggaaa
     1621 tatttggttt agatttatat caagttgaga taggaaagaa aattgaaaaa atcttttgcg
     1681 aaatgctagt aggtcctcaa gcagttcgta aaacaatcaa taaatatata acagaagttg
     1741 gaggaaagta actatggaaa tgacttttag atggtatggg gcaaatgaag aaaaaatctc
     1801 actacaagaa attcgtcaga tcccagcaat gaaaggaatt gttggaacat taatggatat
     1861 accagccgga gaggtctggc cgagagaacg aattaaagct ttaaaagatg aagtcgaagc
     1921 caatggcctt accttgaaag ttattgagag tgttaatgtt catgatgata tcaaaattgg
     1981 tttgccaaca cgagatcaat acatcgagaa ttataaacag acgattcgca atttggcaga
     2041 atttgggatt gaggttatct gttataattt tatgccagtc tttgattggg taaaatcaga
     2101 tttggattat catttagatg atgggtcttc tacgttagcc tatatttcaa aaaatattcc
     2161 tactgatcca gaggaaatta ttagaaaagt agaaaattct actaataatt ttgaattgcc
     2221 gggttgggaa cctcatcggt tatcacaaat caagcaatta ttagaagcct ataaagaagt
     2281 tgatgaagcg aaattgcgag agaactttgc atattttctc aaagcaatta tcccgacatg
     2341 tgaagaagtt gggattaaaa tggcagttca tccagatgat ccaccatatt ctattttcgg
     2401 attacctcgt attgttaaaa atcgtgacga tttagaatgg ttgtgtaatg tagtggattc
     2461 tccttctaat ggtattacgt tatgtactgg aagtattgca gaacgaccag ataatgatgt
     2521 ctatgaaatt ctatctgaat tttgtcaacg ggatcggatt ccttttgcac atgttcgtaa
     2581 tatcaaatat ttaagtgata atggactcga tttttatgaa gcgccgcata aatcagaata
     2641 tggttctcta gatatgtata agatattaaa aacaatgtac gataatggat ttaatggcta
     2701 tattcgtcca gatcatggtc ggatgatttg gggagaaacc ggtagacctg gttatgggtt
     2761 atatgaccga gctctaggtg cagtttattt aaatggtatt tgggaagcgc ttgaaaaggg
     2821 ggcgagtaaa taattatata atggagtcta tttagaagaa aaattttctc ttctaaaaac
     2881 taagggaagg aaaggagtgc ttaaatatgt ttttagataa gaacttttta ttagagaatg
     2941 aaatagctaa aagacttttt cataaatttg cagaacacca accaatcatc gattatcatt
     3001 gtcatttaga cccagcatgt ttgctagaaa ataagcaata caagaatttg actcaggtat
     3061 ggttaaatga taaaggagct ggagatcatt ataaatggcg attgatgcga gctaatggga
     3121 tagaagaaga gttgatcagt ggtgcaggta atgattatga aaaattttta gcttttgtaa
     3181 aaacagtgga gaaggcgcca ggtaatccta tttttgaatg gtctcatttg gaattacaac
     3241 gttactttga tattgatttg acaatttctg agaagaatgc accagaaatt tggcgacgtg
     3301 ccaacgaaaa aatccaaacg gattcctatc gcccaaaaga attgctgaag atgatggatg
     3361 tgaaagccat ttgtacaacg gatgatccta cagatgactt gcgatatcat actgagcttt
     3421 ctaaacagga taattctttc aaggtattac ctactttccg tccagataag gtctggagta
     3481 tcaaatcatc aggattttcg aactatattt taaaacttag tcaagcagcg ggtataccaa
     3541 tcaaaaactt taatgggttg aagcaggcat tgagccaacg agtggattat tttcatcaaa
     3601 ttggctgtcg attagcagat cacggtttga atacttattt ttatgaaata gcagatgaaa
     3661 aaatactcga caatctgtta ataaaagctt tagcaggaaa aacggatttc catttatcag
     3721 aaattgcaca atttgttaca ggtattcaat tacatttgat gtctgagtat acaagatatg
     3781 gttggacgat gcaaatgcat ttaaatgctt taagaaataa ttcaagtata ttaaaggaac
     3841 aaattggagt agatgtcggt ggtgattcga tgggggatca agctagtttg acacaaaatg
     3901 ttgtagcatt gttcgacact gctgaaaaga gtgggtgctt accaaaaaca attttatatt
     3961 cattaaatcc aaccgattgg cttccactag caacagcaat gcaaagcttc caagggggta
     4021 tgattcaaaa aatccaattg ggatgtgctt ggtggttcaa tgatacagct gaaggaatgc
     4081 gtcaacagtt gactacgatg gcgcaacaaa gtttgttgtc gaattttaca ggtatgttga
     4141 ctgattctcg aagttttcta agctatcctc gccatgagta tttccgtcga gtattatgtc
     4201 aattactaag cgagtgggtt aaagcaggtc gaattccaga tgatgaaatg tatcttagtc
     4261 agatagtgga gaatatttgt tatcgtaatg cgtataatta ttttaatttt tttgaaatcg
     4321 aaagtagaaa ataatacaaa aaataaaagc ttaaatttat ttattgtaag ttagtacaac
     4381 tagctagagt atgtatccat aaaagaagga gatagatatg aaaaaagtag aaattttaag
     4441 taagttagaa gcggctggag tcattgcggt tgtacgtgga aaaaataaag atgaggccat
     4501 taaagcaata aatgcaatta ctgcgggtgg aattaaaggg attgaactaa cttttacagt
     4561 tccgaatgct gaagaaataa tcaaagaaat tgctaaaaaa tatgctgatc aaaaagatgt
     4621 aataattgga gccggtacag ttttagatgc gtttactgct cgtattgcaa ttatggctgg
     4681 agcagaatat atcgtaagtc catcttttga tcgacaaaca gcagagattt gtaatttata
     4741 tcaaattcct tatttaccag gttgtatgac aattactgag ataaaggagg cacttaccgc
     4801 tggagtagaa atcattaaac tatttccagg ttctgctttt ggtccatctg ttgtaaaaac
     4861 gtatcaagct ccattaccat atgtaaatat aatgcctact ggcggagtaa gtctagataa
     4921 cttagaagag tggtttgaag caggcgttgt tgcagttggt gtaggtggga atttgttggc
     4981 tcctgttgaa aaaggtgatt ttgaagaagt aactcgaata gctagtaaat atatagataa
     5041 gttttatgag ataaagaatt attaggagaa aaaataaata atactataa
//
LOCUS       DAYYCY010000162         5061 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000162 DAYYCY010000000
VERSION     DAYYCY010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.163"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(205..660)
                     /locus_tag="ACHAZB_002386"
     CDS             complement(205..660)
                     /locus_tag="ACHAZB_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LMxysn_1693 family intestinal colonization
                     protein"
                     /protein_id="HFM8615009.1"
                     /translation="MKKQLFKTFLLSTSILTFGSAITPSGAVFANENNISDIATYSDD
                     EYYYVLDEPVIQTRTWSKPQSWSIAKGSRTYLGIETHSRGTKLSVSANVFGMKIEIAR
                     NTSASGKFKKYRQNARITVTYRVYRKVDNKYIRTQTSTANTQYIDYIPA"
     gene            complement(1063..1236)
                     /locus_tag="ACHAZB_002387"
     CDS             complement(1063..1236)
                     /locus_tag="ACHAZB_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8615010.1"
                     /translation="MLKKTVLVLGSISLGILGFSPMLLSVIGKNNSNRELKLNSNYYN
                     SPNEELFVKETDK"
     gene            complement(1917..2456)
                     /locus_tag="ACHAZB_002388"
     CDS             complement(1917..2456)
                     /locus_tag="ACHAZB_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFM8615011.1"
                     /translation="MADKTIKELAEELGVSKTAINKKVSDKNRKLWFAKNGNKFVINE
                     TGQKAIKSMFLVDNANLNRKLVGEKSKTKNHKNTNQVSELKLLSFLEKQLNRKDIQLQ
                     EKDNQIKQMQKLLDQQQQLTLQANQQIENLQEQLHLTYTEETPTTESTAFSEKKADNT
                     QPLIEKKWWQIWKSNRTNL"
     gene            complement(2472..3395)
                     /locus_tag="ACHAZB_002389"
     CDS             complement(2472..3395)
                     /locus_tag="ACHAZB_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFM8615012.1"
                     /translation="MANEIVKYENRLNHIPLRKFNSREMNLFFSIASRVREKGVDEII
                     FSFHDLKKLSGYTERGEQFIKDLSSTYDKLLSINAWNDDGNTLTKFVAFTKYSIIRDK
                     QLVIISVNPEFKGLFNQLKNWTRFNLEQFTNLDSTYSKTMFRLLKQYRTQGWTEFSKE
                     KFFDLLDIPKSYWNSPSNIDKFILRPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFT
                     WKAEINHADDFSKGKQEDLRIKLFNIDHNGELTREEKWRAKDRILNLPLGTHEADFKQ
                     QQQTEEKQAEKRELSNKLKQDLLGNLQNLFD"
     gene            complement(4523..4678)
                     /locus_tag="ACHAZB_002390"
     CDS             complement(4523..4678)
                     /locus_tag="ACHAZB_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8615013.1"
                     /translation="MKLTKLTISLVIIAGIILYFVLPANQFFLYFGIPTILIVNGVRI
                     FEQRKKM"
     gene            complement(4697..>5061)
                     /locus_tag="ACHAZB_002391"
     CDS             complement(4697..>5061)
                     /locus_tag="ACHAZB_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HFM8615014.1"
                     /translation="FSVLFLINILNTSFSEGINPNEVNGSLLLFFLNAATYGAFLEEG
                     IFRFCMIDPQANKKQQYISILISSFLFSIVHGGGLSIFFIGIILSFVYIQTKNIWYSI
                     VAHGFYNTIGILIYLISI"
BASE COUNT         1607 a          839 c          771 g         1844 t
ORIGIN      
        1 aaaaaaaact atatttaaag agttttttta gatttacttc tttgaattca cgaatgaagg
       61 tagaatgaaa tagtataatt attagcacta aaaaaaatat atcactatgt tcaaataaaa
      121 aatatatatt tgacggtact cctaatgatg ggatagcaaa ggtcaaaacc aagtaagcaa
      181 tactaattat ccattttatt gtcattaggc aggaatataa tcaatatatt gtgtatttgc
      241 tgtagaagtt tgagtacgaa tatatttatt atctacctta cgataaactc tgtaggttac
      301 agttattctt gcattttgac gatatttttt aaattttcca cttgcagaag tgtttctggc
      361 tatttcgatt ttcatcccaa atacatttgc actgacagat aactttgtac ctcgactatg
      421 agtttctata cctaaataag tacggcttcc tttagcaatt gaccagcttt gaggtttaga
      481 ccaagtcctt gtttgaataa ctggttcgtc taacacatag tagtactcat catcagaata
      541 agtggctata tcagaaatgt tgttctcatt agcgaatacc gctccgctag gagtaatagc
      601 agaaccaaaa gttaaaatag aagtagataa taaaaaggtt ttaaataact gttttttcat
      661 caaaatcacc tcgaatataa gatacttaag aaaacgcttt ctttatataa ttagaataat
      721 caggaaattt aatgttgtca agagggttta ttttataaaa gctgttatat taaaggttag
      781 tagacataat gtacagtatg ccaagtagcc cttgtatcaa ccgatacagg ggctcacttt
      841 gtgattttta attggtctag gtaaaaagct ctttttaagg cttgattttc tgcagttgct
      901 tctttccgtt tctttgtgaa tttttaccag tcatttttta ctggattgtt atttttcata
      961 aaatatgata aatttttggt agcgctttat aagcgcgttg ctttttcaag aataaacact
     1021 cattattttt taactggctg ctcggaagag cagtcttttt tattatttat ccgtttcttt
     1081 tacaaacaat tcttcgttag gactgttgta ataatttgaa ttcaatttaa gttctctgtt
     1141 ggaattgttt tttccaatta cggataatag catggggcta aaacctaata ttcctagaga
     1201 aatagaaccc agtaccaaca cagttttttt taacataggt tatacctcca ctttttttaa
     1261 cgctaccact tgtatacaca ttttaaatac tagccttcag tttatcaaat gtttaaattg
     1321 aacggatata aatcaaaatt aaattttcaa aagttgttct aaaaaatttt tcttttttgg
     1381 aagttactct aacttttttt gcttgtggtg cttggtatgt tttctccgcc tcccaggttc
     1441 ttgaccattt tctcttataa gtgggatttt taccacgttg agagaaaatg gtcaagaacc
     1501 tgggaggcgg agaatgccct taataccaag caccgcaagc ctttcccata ctttattcga
     1561 tgatcccacg atgatctcat aatgattcta caatgaccct acgatgatca aaattctcaa
     1621 aaaaaataaa cgaaatttca aacgattttt ttgaaatttt gttaaaatca catttgtttt
     1681 tttatgactt tttcaacaaa atcgattaga atttattttg tttttccacc aaaaattgag
     1741 tgagatttca acctatttta agcaataagt caccatatag tttgatagtt tacaactata
     1801 aacattgatt gtttacagtt gtaaactatt ttttctttct cataaaaatt tgagtgatct
     1861 ccccatcagg tagtacaact ttttcctcta ctatccgaac taacttctag aaaattttag
     1921 agattggttc tattagattt ccaaatttgc caccactttt tttcaataag tggttgtgta
     1981 ttgtctgctt ttttttcaga aaaggcagtt gattcggttg ttggagtttc ttctgtataa
     2041 gttaaatgga gctgttcttg taggttctca atttgttggt tcgcttgtag agtgagttgt
     2101 tgttgctggt cgagtaattt ttgcatttgt tttatttgat tatctttttc ttgtagctgt
     2161 atatcttttc gatttaactg tttttctagg aaagaaagga gttttaattc ggaaacttgg
     2221 ttggtgtttt tatggttttt ggtttttgat ttttcgccaa ctagttttcg atttaagttt
     2281 gcgttatcga ctaaaaacat tgattttata gctttttgac cggtttcgtt aatcacaaac
     2341 ttgtttccgt tttttgcaaa ccaaagtttt cgatttttgt cagaaacttt tttgttaatt
     2401 gctgttttac taactcctaa ttcttccgca agttctttga ttgttttatc tgccataaat
     2461 actccttaag cttaatcgaa taagttttgt aaatttccta gtaaatcttg tttgagtttg
     2521 tttgaaagtt ctcgtttttc tgcctgtttt tcttcagttt gttgttgttg tttaaaatca
     2581 gcttcatgag tgcctaaggg aagatttagt attcgatctt tagcgcgcca tttttcttct
     2641 cgtgttaatt ctccattgtg atcaatatta aataatttta tacgtaaatc ttcttgtttc
     2701 cctttggaaa aatcgtcagc atggttaatt tcagctttcc aagtaaaacg ataaccgata
     2761 acaggtttac ctcgaccttt accatatttt ttacgaatag ttaatccttt aaataaggga
     2821 gtgagttctt ctttaattgg ccttaagata aatttgtcaa tatttgacgg actattccaa
     2881 taacttttag gaatatctag taaatcaaaa aatttttctt tggaaaattc agtccagcct
     2941 tgagttcgat attgttttaa taatcgaaac atagtttttg aatatgtgct atccaaatta
     3001 gtaaattgct ctagattaaa tcttgtccag ttctttaatt gattaaaaag tcctttaaat
     3061 tctggattaa ctgaaattat aacaagttgt ttatctcgta taatgctata ttttgtgaaa
     3121 gcaacaaatt tagttaaagt atttccatca tcattccaag cattaatact taataatttg
     3181 tcatacgtgc tactaagatc ttttataaat tgttccccac gttctgtata accgcttagt
     3241 tttttcaaat cgtgaaatga aaaaataatc tcatctacac ctttttcacg tacacgtgaa
     3301 gcaattgaaa aaaataagtt catttcacga ctattaaatt ttctcagagg aatatgattt
     3361 aatctatttt catattttac aatttcattt gccataagct tctcctactt ttataaagta
     3421 tgaaataaaa gtaacatact ttataaaaat actcaataat gtcggaaaac ctacttgaaa
     3481 agcgtagaaa acctacttga aaagcgtaga aaacctactt gaaaagcgta gaaaacctac
     3541 ttgaaaaaac tctgaattgc ttgggagagt aaggacacag ccgacataaa agagatataa
     3601 aaagagatat aaaaagagat ataaaaagag gggaggctaa ttttcccgcc taaaaaatca
     3661 ctcctttttt ctctaaacag gttcgcgaat ttatcaaaac tgccaattcc aaaccttgct
     3721 ccctacggtt cgctaaaaag gtgtgatcgc tctgctctca cattatactg cgcagtcgcc
     3781 tccggctcct ttgctaaaag ccgttcataa ggaacccttt gagaagctct gagcgttcgt
     3841 ttaagacatt ttaagacagt cctagtataa atcccttatc ttgttcttaa aacgccttac
     3901 aggcggtcag ggcaagccct aacaacccta aaattgtcta aatgccctct aaaattcgat
     3961 ttaagcactc ttaaacctat tcttatatat ttatatactt aggcgtttca aacgtcttaa
     4021 aacgcaaatt agagcttgat aaagatattt gctcgtgtgc ctagactttt aaaaaagtcg
     4081 gcacacgcca tgacaaagct gaaataaact gcttcagctt tgtcatggcg gattcgctca
     4141 cttgaaaaag tgtttatttc gctcgattat tagcatatat tttttagctt ttttgctagc
     4201 caaattttgc attaatttgc tcataagtcc caccctttca gtacgtccta cggacgtatc
     4261 gttcgtaatg tgaaagtacc tttaaaggta ctttctacaa aactcactaa aagggtttgg
     4321 caagctctat atttgctagc caaataccta cgtatttggt gcaaataacc gcttgtcaaa
     4381 ggggcgcaag ctcccccttg aatcccccct caaaacccac gctccacaat acaaaaaagg
     4441 ctagcaaatg ctagccactt tttgtaaagt ttcgcttgtt tcaccttcgg tgaaattgtt
     4501 gctttgcaac gatcaggtag agctacattt tttttcgttg ttcaaatatt cttacgccat
     4561 ttactattaa aatcgtaggt attccaaagt aaagaaaaaa ttgattagca ggtaatacaa
     4621 aatataaaat aataccagca ataattacca aacttatagt aagttttgtt aatttcatga
     4681 agtatgccac cttatcttat atagatatca aatagattaa tatgccgatt gtattataaa
     4741 atccatgagc aactatggaa taccatatat tttttgtttg tatataaaca aaacttaaaa
     4801 taataccaat aaagaaaata cttaggcctc ccccatgaac aatagaaaac agaaaagaag
     4861 aaattagtat tgaaatatat tgttgttttt tgtttgcttg aggatcaatc atacaaaatc
     4921 gaaaaattcc ttcttctaaa aaagctccat aagtagcagc atttaaaaag aataaaagga
     4981 gagaaccatt tacttcatta ggatttatac cttctgaaaa agatgtattt agaatattga
     5041 ttaggaataa aacagagaat g
//
LOCUS       DAYYCY010000163         4986 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.164, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000163 DAYYCY010000000
VERSION     DAYYCY010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4986
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.164"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(243..1217)
                     /gene="sgrA"
                     /locus_tag="ACHAZB_002392"
     CDS             complement(243..1217)
                     /gene="sgrA"
                     /locus_tag="ACHAZB_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HFM8615015.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(1413..2252)
                     /locus_tag="ACHAZB_002393"
     CDS             complement(1413..2252)
                     /locus_tag="ACHAZB_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFM8615016.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            2437..3324
                     /locus_tag="ACHAZB_002394"
     CDS             2437..3324
                     /locus_tag="ACHAZB_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFM8615017.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(3908..4603)
                     /locus_tag="ACHAZB_002395"
     CDS             complement(3908..4603)
                     /locus_tag="ACHAZB_002395"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFM8615018.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(4587..4985)
                     /locus_tag="ACHAZB_002396"
     CDS             complement(4587..4985)
                     /locus_tag="ACHAZB_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFM8615019.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         1581 a         1013 c          742 g         1650 t
ORIGIN      
        1 acgtacaaat agattcgtta ctgatttttc agagatacta ccatgcgcag tgtgcattgt
       61 caattattct tgctcttatt ttccatttgt gttcctgtta tttcctgttt ctcaaatatg
      121 ttagtaaaca aaaatagatc tggcaaagct acaacttcgc caggcaatac agcactctcg
      181 aatgaaatat tcactcaaaa atctatcgtt ttattcaatt ttagatctgt tgcttttatt
      241 ctttatttca ctcgatggaa gagagaacga aaatagcttg ctcctacaat caaaatgagt
      301 aacacaccca tccatgtaat tagcttgttc aaggttctac taccagtttc tggtaaatcg
      361 ctttttctat tatctgtttc tttcgctttt tcttgatgat caattccacg agaaacaaaa
      421 tcaggctgtt ttagcacact ttttaggttg ctccctgcat ttctcttttg ttccttagtt
      481 ggtatgatag gtaccaaatc aagttcatta tttacttttc ctgtgctgct ttcgctgctc
      541 gtgcttcctg tgctactctc actactcatg cttcctgtgc tactttcact actcgtactc
      601 tccgtactgc tttcgctgct cgtgcttcct gtgctacttt cactactcgt actctccgta
      661 ctgctttcgc tgctcgtgtt ttctgtgcta ctttcactgc tcgtactctc tgtactgctt
      721 tcaccgctcg tgttttctgt actgctttca ctacttgtgc tatccgtact gcttggcggt
      781 ccaacaactg tccaactgct tggtttcaaa ttctccgtga tcgtcatctg tccatcataa
      841 aaaacaatca aatcaacttt atctttactc ttgatctcat caatcgatag ttctgcagaa
      901 atcattattg tatactcaat accagcggtc cattgtacgt cttttaaata aacttcccca
      961 ataacttttt ctgaacttcc attaccatcc caaccatatg ttagaacagc atgtacatca
     1021 ctagcattca cgcttttgtt tggtttgact ggaatagtaa tgacttgttg tgtcatgtca
     1081 taaacaatcc gatcagtttt cacagtaaat gaaagattct catttgcccg ttcatttgca
     1141 gaagtactga ttccaatcat tgctgcaatt atactcagcc caagaataac aaataatacg
     1201 gtcgctgttt tcttcatttc tatcactctc catataatta ccttctattt gataaaggaa
     1261 ttatataaaa tgaaaatgat gagaacacga cagtcagttg tgaaaatcta acaaaagatg
     1321 tcgtgtattc gcttgaattt gtttactttt tcgtttaatc aatgacagac ttaaaaaagc
     1381 aagaccgata tcagccttgc tttcttcatc ttttattgat aaacagcagt gtatttcttc
     1441 atccgctttg ttttatatgc atagagtaac aaaccaacaa tcaagactaa caagaataat
     1501 gtagttgata tagatgcttc agccccaaag tttcccccag taattattgt attttgcgat
     1561 tggatcagag tggagaaaaa accttgttct tgcgttccgc tgactggtat acccaaaata
     1621 gctccttgtg tccaattcca agtaatatga gcaccaatag ccgtaaataa acttcctgta
     1681 ctgtagataa gtgcgctgaa aaagcaacct gctaaaaaga tattgaaaac tgccagaaat
     1741 gtcatgttag gattcaatcc gtgcaaaaga gcaaataatg cagcagatcc aatcattgct
     1801 attaacggac caaatctaag catctgatgc tgaaaaaagc cacggaataa aagctcttct
     1861 gatgccccca ctgcaataaa taaaataaaa ctcgttatca gcggcacaat gtttgcgttt
     1921 cctgaatcta accgatacgc ccccgtcaaa tataatggac tagtaataag cagaaacact
     1981 ccaattccaa caacgagacc tcccgctaaa tacccccagt cttttcctcg aacagaaaca
     2041 ccataatctt ctattctttt tccttcgacc ttactagcaa gaaataaagc tagtaaaaaa
     2101 acagctaaat gtccgaccgt accggcaatt gctccaatcc attcactatt tccaataaac
     2161 atatccgcaa gtgcattatt gacaagaatt cctaaaacaa aaccaactaa gactattaaa
     2221 acagatccaa tcaaccgaat aatcctgatc atctttcatc cctcctttat attaagttta
     2281 acaacccttt tctttcttgt ctcgtgaaaa ctatgtaaat gttctccatc atagagagaa
     2341 tagattttta cgaactaaga atatctacag caaccttacg aaaatgaaag actgtctccc
     2401 taattcttct gttagactgt ttttaataag gaaaggatgg gaaatttttt gaaaaaacaa
     2461 ctactaatta tgtgcctttc agcatgtgca attttactat tctcaggttg tagcaagtcc
     2521 aatgaaacga ttgcaacgtt taaaggtgga acacttacag ttcaggattt ttatgataac
     2581 ccaaaagtta aatcaaataa tcaaacacta ttaaaaaaaa tgattgtctt caaagcattt
     2641 gatgatgtat acggaaaaga gatttcttca aaggaagtaa caaaagaata taatgaacaa
     2701 atcaaaaaac tcggtccaaa ctacaaggaa caattaaaag cagttggcca aacagaagag
     2761 acatataaga aactctttaa acagatgctg gctttccaat acggtttgaa agccaatgta
     2821 aaattaacag ataaagatct agacactgct tggaaagaat tctatccaga agttagtaca
     2881 cagatcattc tattttcgac taaagaagaa gcagataaag caaaaaaaga agcaaatgaa
     2941 ggggaaaatt tctccaagct tgttcaagct tatggaaaaa ataaaacatt aaaggaaact
     3001 gatggaaaaa tgaattttga ttcaaccaat ccagagattc ctactgaagt aaaaaaagca
     3061 gcatttaaac taaaaaacgg ggaagtttct gatatcattc ctgtaacaga tccaacgact
     3121 tatcagcaaa gctattatct agtaaaaatg gtaaaaaaac aggataaagg ttctaataaa
     3181 gacaaataca aatctgagct agaaaaaatt gctaccgaag caaaaactct tgatacggaa
     3241 ttcatggata aaacaatccg taaagtgatg aaaaaagaca atgtaactat taaagatcct
     3301 tatgtaaaaa atatattcaa ataaacaaat ttgacccaat tggggatgtg gcaaaatttg
     3361 agaagtgatt tttggaaatt ccttcttatt tttgaggcaa accacttttg ccacaacttc
     3421 atcggggcct ttttttattt gtaccaaaac aaatcactgg atttgttaca atcatgagga
     3481 tataatttaa aaaagttttt tatttctatc acatgaatca atgcgccatt aagtagattc
     3541 tttcatgctt ccctattttt tcacttgccg tatcgcttca aaaacaacta tcataaactc
     3601 aaactcgact gaaaagttat tttaaaaaag actaaaaata tcccttctgg acttgttcct
     3661 cttattctgt tatactagag aaacacgaaa gatattttta tgcgacactt ttcatttgtt
     3721 tttatgaaat ttattcaaaa ctggcgtaac ttcagagtta caaggatgta ttagaggtta
     3781 gggaatacat cgtttaaatg agaaataagt aatagtcacc caaactttaa attaatgaat
     3841 attgaatcgt cagatagcct cctcgagttt tcgagaaggc tatcgtttat tttatggaga
     3901 aattgattca gaagtaatca gcgacctttt ttagttcacc aatcgtttct gcttggaaag
     3961 tttgcaccca atcttcttgg aagcccctca ctgctttatc aatcgagggc atctctaata
     4021 cttgatctag tagctcttta cctaacgtta tcgcatgagc tccagatcga atggctgtag
     4081 tgacttgttc acaatttttg aaactagctg ctaatatttt tgttggtctt tgcgtacgtt
     4141 cgatctcagt ccgcaacgaa tcaatcactt tcatagcatc aatgccttga ttttgcatcc
     4201 tgttataata aggtgcaata taatctgctc cattattaat agctaaatac ccttgtaatt
     4261 ctgtgtaaat agctgtaccg gtaatcaaat aaccttcttt tttcaacttt ttgatcgctt
     4321 gaaggcctgc ttgattcacc ggcaccttga taaacgtatc ttttcccagt tcttctatga
     4381 taactcgagc atcctctatc atttcttctt ccgttcttcc tattgtctga atatggatag
     4441 gaatctgtcc gattattttt ttgatttctt tcatatgcga gaagaagttt gacgctcctt
     4501 cttttttcag aattgtagga tttgttgtta cgcctgttaa attcaaaaac tgttgacaag
     4561 aataaatact ttttgtattt gctgtatcaa gcatgatttc catatagctt tcccccattc
     4621 tgatcctctt gatcgatttt aatgattgtc ccctttctta aatgcggcat ttccttatct
     4681 tctacataca ttgttcctgg aagttccggc tcatgagatc catcaaattt tatcgtgata
     4741 tgtcctaatt cattcaaatt agtttggaca acatctccta ctgctgtgat tacatacatt
     4801 tgtttatcaa aacagagctt catatcacaa gttatttctt ttcttacctg attcaattca
     4861 atcttataac aataatctgt taatgtttcc ggtgcttcat ctccaaataa aatcattatt
     4921 ttttctttcc tgaatacttc tgcttctata ccaacatctg ttacaaccgt ttcaaagatc
     4981 gtcatc
//
LOCUS       DAYYCY010000164         4976 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000164 DAYYCY010000000
VERSION     DAYYCY010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4976
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.165"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            13..225
                     /locus_tag="ACHAZB_002397"
     CDS             13..225
                     /locus_tag="ACHAZB_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8615020.1"
                     /translation="MKMKIFKKFFAKNKISNSALIEKSLLLSIFFPTNNNKSEKEKSD
                     KKIAYPLLLLLFSFYQVQKLFCFVIC"
     gene            complement(202..1812)
                     /locus_tag="ACHAZB_002398"
     CDS             complement(202..1812)
                     /locus_tag="ACHAZB_002398"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HFM8615021.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(2034..2279)
                     /locus_tag="ACHAZB_002399"
     CDS             complement(2034..2279)
                     /locus_tag="ACHAZB_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8615022.1"
                     /translation="MKEKDCFYDKLYKLLLQVGKSSSLLGGILSIIMFVLFFYLLFHL
                     KFFSNDFLICLGLTLAMFISSLLCGLNFFCTHKGLKK"
     gene            complement(3005..4345)
                     /gene="glnA"
                     /locus_tag="ACHAZB_002400"
     CDS             complement(3005..4345)
                     /gene="glnA"
                     /locus_tag="ACHAZB_002400"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HFM8615023.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(4373..4765)
                     /locus_tag="ACHAZB_002401"
     CDS             complement(4373..4765)
                     /locus_tag="ACHAZB_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM8615024.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT         1480 a         1064 c          807 g         1625 t
ORIGIN      
        1 ctactaaaat caatgaaaat gaaaattttc aagaagtttt ttgcaaaaaa taaaatatcc
       61 aatagcgctt taatcgaaaa aagtcttttg ctttctatct tttttcctac aaataataac
      121 aaatcagaaa aagagaaaag tgacaagaaa attgcttatc cccttctctt gctacttttc
      181 tctttctatc aagttcaaaa attattttgt ttcgtgatct gctaatgatg ctccgacaaa
      241 tgctttgatt aatttttgcg ggcgatttgg acgagaaatc aattctgggt ggaattggca
      301 tcccacaaag aattttttct ctggcagctc tacgatttct actaaacggt tatctggtga
      361 gacacctgaa aagactagcc cttgttcttc aaacaattga cgatatttgt tattgaactc
      421 atagcggtga cggtgacgtt cttgcacaac atcggctcca tcatatgctt cagccgtctt
      481 cgttcctttt ttgattttac atgggtataa tccaaggcgt aaagttccac ccaaattctc
      541 gatgttttcc tgatcagcca ttaaatcaat aatattgttt gctgtatctg gattcgtttc
      601 agctgatgac gcatcttcta atccaacgac gttacgtgca aattcgacac aagccatctg
      661 cataccaaga caaataccta aaaacggaac gtcattttcg cgagcataac gaatcgcttc
      721 gattttccct tctactccgc gatcaccaaa tccaccagga accaagattc catctgcgtc
      781 tttcaaacgt tcttctacat tttctctcgt taattcttgt gctttgatcc aatcgatctc
      841 gatgtctgaa ttataggcga aaccagaatg tttcaatgct tcaactactg agatatatgc
      901 atctggtaac tcgacatatt taccgacaag cgcaatccgt acttttttct tcaagttcaa
      961 gacacgttct tctaattctt tccattctgt catgtctgct tctggtgtat ctagttttaa
     1021 atggtcgcaa acaatttgat ccattccttg tgcttgtaaa actaatggga tggagtaaag
     1081 tgtatctaca tctggcgact cgataactgc ttctggttcg acatcacaaa attgtgccaa
     1141 tttgtttttc acgctttgag aaacaggttg ctctgtacgt accaccaaaa tattcggttg
     1201 gatacctaaa ccacgtagct cttttacact gtgctgcgtt ggtttagttt tcatttctcc
     1261 agcagctttc aagtaaggaa tcaatgttgt atggatatac ataacattat ctgtaccgac
     1321 atctgctttc atttgacgaa gtgcttcaag gaatggcaaa gattctatat cccctactgt
     1381 tccccctact tctgtaatga tcacatctga atcagtcatt ttagctgctc gcatgatttt
     1441 ttctttgatc tcatttgtaa tatgaggaat aacttgaaca gttgctccca agtattcgcc
     1501 cttacgttct ttacgcaaaa cttcagaata gatttttcct gtcgtcacgt tggagtattt
     1561 gttcaaattg atgtcaataa aacgttcgta gtggcccaga tccaaatctg tttcagcacc
     1621 atcatctgtt acgaatactt ctccgtgctg atatgggctc atcgtccccg gatccacgtt
     1681 gatgtatgga tcaaattttt ggatagttac ttttaagccg cggtttttca ataagcgtcc
     1741 taaagaagct gcaacaatcc cttttccgat tgatgaaacg acaccgccgg tcacaaaaat
     1801 atattttgtc ataagttcaa aaactccttt ttaatttatg tgcaggaaaa gtctcagaca
     1861 ataaacaaag ctccctattc ttcaataaga atagggagcc gaaatttctg taaacttctg
     1921 accctctaaa agggtgccca aggtgtataa tacaagctga ctaagggaaa gtcaagtact
     1981 caaatgaata ctatccgctt acaatttttt ctttttcgat atcctttgag aaattatttc
     2041 ttaagtcctt tatgagtgca aaaaaaattt aacccacata ataagcttga tataaacata
     2101 gctaacgtta aacctaagca aatcagaaaa tcgttactga aaaattttaa gtgaaataat
     2161 agataaaaaa ataaaacaaa cattatgata gataatatcc cacctaacaa agaactactt
     2221 tttcctactt gcagcaacag tttatatagc ttatcgtaga aacaatcttt ttctttcacc
     2281 cttatttacc tcctctacgt aaaaaacgta gtccacagtt ctcggatttt tggcatatct
     2341 tttctattaa ataatatcaa aaacaaaaca attccactaa ttttttcaaa gatgaccttt
     2401 ttttcaacaa aagtgccttg tagggattca ttgaaggtaa cgaatcacat aagtaaaaaa
     2461 taaggagatg cgacaaaagt cgtgcctcct tatttctaaa taaacggtgt tctcctgcca
     2521 actcctcggt cacaagtcac aatatccatc aaacatgaga agctgttttt gaaattattt
     2581 cttgtgattc ggagttaaat ggtaggctca caacctctgg ataaacggta ttcaaaagcc
     2641 agtttctcgg tattaagcca aatttatgca aaaatgtaga aagccatttt cacaaaattt
     2701 ttcttaatac tcgaaactaa acgtcttttt caaaacctct tggtaaacgg tgttcaaaag
     2761 cttgcatctg cggtaataag ttcagccaat tcaaaaacaa gaaaagctgt ttttgcttgg
     2821 ctattcttat tacttgttgc agggcagctt tttcacaacc tctccactaa acggtgttca
     2881 atcagctgac cctcggtatt agctcaaaaa acggcaaaat atgaggaact atttttccgt
     2941 tagtttctct aatactcagg tcaaaacgct gatttcacag cctctaattc acggtattcc
     3001 tgttttaata aagttctaga tattgttctc tttcccactc ggaaacagtt tgtctgaatg
     3061 ccgcccattc gagacgtttt gcttcaacaa aatttgagaa gatatgttgt cctaatgcgt
     3121 caatcattac attatcctta cgcaattctt tgatagcatt atggatagtt gaaggtaagt
     3181 cctggatatg tgcagcttct ctttcttcct catccattac ataaatgtta cggtcaacag
     3241 ctgctggcgg tgtgatctga ttacggatac cgtctagtcc tgcttgcaac aatactgcca
     3301 ttgccaagta tgggtttgca gatggatcaa ctgaccgcaa ttccaaacgt gtggataaac
     3361 cgcgagattc tggtacacgt actaatggcg aacgattacg gccactccat gcgacataaa
     3421 caggcgcttc ataacctgga acgagacgtt tataagaatt gactgttgga ttgcaaacgg
     3481 ctgtgtaggc acgagcatgt ttcagcaaac cacctaagaa atgataagct gtttcactta
     3541 gttccaattc accgtcttta tcatagaaaa catttccttc atcattgaac aaagacatat
     3601 tgcaatgcat accagaacca ttgatcccaa ataacggttt cggcatgaaa gtcgcgtgaa
     3661 gtccatgctt gcgtgcaatc gttttgacaa cgagcttgaa ggtttggata ttatcacaag
     3721 catctaccac atctgcatat ttaaagtcaa tttcatgctg tcctggcgct acttcatgat
     3781 gtgaagcttc cacttcaaag cctagacttt ctaattccaa cacgatgtca cggcggcaat
     3841 tttcacctaa atctgttggt gcaaaatcaa aataaccacc tttatcattc aaggtcgttg
     3901 tgatcccacc gtcttcgtct aatttaaata agaagaattc tggttcaggt cctaaattga
     3961 aagaggtaaa accaaggtcg cgcatatctt tcaatgctcg tttcaaattc ccgcgaggat
     4021 cgccggcaaa tggtgtccca tccggattat agatatcaca aattaaacga gcaacttttc
     4081 catgagcact ttcccacggg aagatcatcc aagtggagag atctggatac aaatacatat
     4141 cgctttcttc aattcgaaca aatccttcaa tcgaagaacc gtcaaacatc attttatttt
     4201 ctaatacttt gtcgatctgg ctcactggaa cttctacatt tttgatcgta cctaagatgt
     4261 ctgtgaacat caatcgtaaa aatcgtacgt tttcttcctc aatgattcgt ttgatttctt
     4321 cacctgttat ctgtttcttt accatggtat ccctcttcct tttttaattt attcagtcaa
     4381 agaattgtct ttgcaatcct tgcgactgat aatgacctgt tttgcttaac ccaccttgcg
     4441 ccaaaatctc atcacgaaaa atttgtcgaa cgtcatgatc tgtcaacggc ttcttgtttt
     4501 ttgcttcttg ttttttcgcc tgtgccattt cgtatacacg tttgatccca gccatattca
     4561 atccctctga aagatagtct ttaatctcaa gcaacatatc aatatcattc agcgaataca
     4621 tacgtcggtt tccttcgctt cgttccggat gaactaaatc ttgttcttca taatagcgaa
     4681 tttgccgagc agacaaatca gtcagcttca taactgtacc aattggaaat acggacatag
     4741 atcggcgcaa ttccttttct cccatcagac gtcccctttc gttttataag ttggctaaag
     4801 tgtaccaatt aaaaaaaata ttgtcaactg catatgttag gttttctaac atgagatctc
     4861 tattttttat aaaataacaa aaaaataaga acacaaaaaa ggtatctagt tgattttcgt
     4921 accagatacc ttttattttt tattatactg tactatcctt tattagtgtt tttatt
//
LOCUS       DAYYCY010000165         4966 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000165 DAYYCY010000000
VERSION     DAYYCY010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4966
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.166"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..395
                     /locus_tag="ACHAZB_002402"
     CDS             <1..395
                     /locus_tag="ACHAZB_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFM8615025.1"
                     /translation="ISLKDESNTTVSDANILANIILDFIFEHHIPFKEGYEILPANQE
                     YYFYKCITKRVCCICGKTGADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKI
                     GVINFKNKYQIKGIKLNQKTIKKLNIGG"
     gene            398..1147
                     /locus_tag="ACHAZB_002403"
     CDS             398..1147
                     /locus_tag="ACHAZB_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8615026.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            1159..1428
                     /locus_tag="ACHAZB_002404"
     CDS             1159..1428
                     /locus_tag="ACHAZB_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8615027.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            1433..1597
                     /locus_tag="ACHAZB_002405"
     CDS             1433..1597
                     /locus_tag="ACHAZB_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8615028.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            1590..1892
                     /locus_tag="ACHAZB_002406"
     CDS             1590..1892
                     /locus_tag="ACHAZB_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8615029.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            1889..2050
                     /locus_tag="ACHAZB_002407"
     CDS             1889..2050
                     /locus_tag="ACHAZB_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFM8615030.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            2047..2352
                     /locus_tag="ACHAZB_002408"
     CDS             2047..2352
                     /locus_tag="ACHAZB_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8615031.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            2352..2708
                     /locus_tag="ACHAZB_002409"
     CDS             2352..2708
                     /locus_tag="ACHAZB_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HFM8615032.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            2668..2913
                     /locus_tag="ACHAZB_002410"
     CDS             2668..2913
                     /locus_tag="ACHAZB_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8615033.1"
                     /translation="MIKKLVQFSMDLYDIESGATLSVESDHLIINFGGKRQIILWVVD
                     DVLFPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            2910..3329
                     /locus_tag="ACHAZB_002411"
     CDS             2910..3329
                     /locus_tag="ACHAZB_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HFM8615034.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            3326..3883
                     /locus_tag="ACHAZB_002412"
     CDS             3326..3883
                     /locus_tag="ACHAZB_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HFM8615035.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVVER"
     gene            3880..4176
                     /locus_tag="ACHAZB_002413"
     CDS             3880..4176
                     /locus_tag="ACHAZB_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8615036.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            4253..4666
                     /locus_tag="ACHAZB_002414"
     CDS             4253..4666
                     /locus_tag="ACHAZB_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFM8615037.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
BASE COUNT         1842 a          773 c         1130 g         1221 t
ORIGIN      
        1 aaataagcct caaagacgaa tcgaatacaa ccgtgagtga tgccaatatc ttagcgaata
       61 tcatcctaga tttcatcttt gaacatcata ttcctttcaa agaaggatat gagattttac
      121 cagcgaatca agagtattac ttctacaaat gcattacaaa aagagtctgc tgcatctgtg
      181 gcaaaacagg agctgacatc gatcactttg acaaagcgct aggaagacga aagcgcaaag
      241 aagttgatca tgcagagtac acatttgcag cactctgcag aatccatcac acagagaagc
      301 acaaaatagg tgtgattaat ttcaaaaata aatatcaaat caaagggatc aaattaaacc
      361 agaaaacaat caaaaagtta aatattggag ggtaaaaatg acagaacatc gaagttatta
      421 tgcgattata ccagccaacg taaggtacga caaaagactt aaaccaaata ctaagttgtt
      481 atacggagag ataacggcct tgtgtaatga aagaggcttt tgttgggcag gcaatgagta
      541 ctttgcagat ttatatggtg tgaataaaga gaccatatcg cgatgggtaa gtgatttgat
      601 taagtttgga tacttgaatc gggaaatcat ttacaaagag ggtaccaatc aaataatcaa
      661 taggtaccta cgaattaatc aataccctat tgacgaaaaa cgcaataccc ctattgacga
      721 aaaagtcaaa gataataata catctattaa taatacattt aataatacaa aagaatatat
      781 aagagagtta ccgccttcga aaaaatcgaa ggctaagccc gtccgtcata aatacggaga
      841 gtataaaaat gttcttttgt cagatgagca gatggagaaa ctcaaaacag aattccctaa
      901 tgattaccaa gagcgaatcg aacgactgtc agagtattgt gaatcatctg gtaagactta
      961 taaaaactat ttggcaacta ttcgaagttg ggcaaggaaa gaaaaaagtg aacctaagaa
     1021 cgcaagcagt ggatacaagc gcacaggaag acgagagaag cttcctgaat gggcaatcga
     1081 ccaagaagcc tatctcaaga aaaaagcgct agaacgagct aatagacaat caaaagcacc
     1141 attctaagag gtggaaaaat gaagatcgat tatctagaac taattaatga aatagcgaat
     1201 tataaaaagg gcgaggaatt agacgtcctg agagacgtat atgatcaact cgaagaagct
     1261 ggaattgaag gaattaagaa tgatcgttcg agttggagta agctcagata ctatttcgca
     1321 ctttatatcg atacaacaca attaagaaat ttagcatata ccaaattact atttgttgat
     1381 tgtgttaaag gcttgcaaaa acatcttaat gaacttgagc aggtgtaatc agatggacct
     1441 aaagacattt acagcacaga ttgaactaat gcatcaagaa gctttaagac aaagtgtatc
     1501 gtacgaagac aagtggctca acacgttcca tggcggacgt gagagcgcac ttgatcaagt
     1561 actcaaatta ctgaaaggag aatgtcggga tggataagaa agcggcaatg cagcgaatta
     1621 tcgaattgac ttattcagaa gattggcaaa atgacaaaga agctgcttca gaagtgatga
     1681 ggcttggaag agcgatgtgg gcagacaaga gcaacaagcc aagaccacga aaaatcgcaa
     1741 tttggcacgg tgacaaactt ctagtgatag ggacagctga acagttagca agtctcacag
     1801 gcttgcacga gaaaatcgtg aggaaaagag caaggtgtgg ctacacagac gctaagaaga
     1861 gaacgtttag atacgtggag gaatcatcat gacaacagaa gaagtgattc aaatgcgtat
     1921 tcgaagcctt cagcgtgaga ttgacgatct ggaacgaaca aaggcagtga tggtcaatga
     1981 aacggcgaga aaggcaatcg atttgcacat agagaattta agaagggaaa tccatcgatt
     2041 ggaggaatga gcgtggataa gaaagcagca atgaaaagaa ttgctgaatt aaccaagtca
     2101 gaatcttggc aagaagacaa agaaatagtt gcagaagtcc aaaagctcgg taaatcaatg
     2161 tggactgaaa agcccaaacg gagaacgccg agaaaaattg caatctggca tgatgaccga
     2221 attttagtaa caggtactgc tgaacagtta tctgaaatta ctggattaag caaaaacatt
     2281 atctgggata gagctaggag cttatggatt gattcaaaag gacgacagtt taggtatgtg
     2341 gaggagagat aatggatctc attacacaat acagtgacat catcctcaag aaaatcatga
     2401 tgaagattca gaaagataaa aaatcaaaag aacgagctga attagttaag ttagaaatgg
     2461 ctgaaacagg agcaggagtg cgaagtagca ggcattggaa agcagcagca aacattgaat
     2521 tttattacaa cgaaattcaa aaagggttcg atcagatgcg tgagctggat cggcaaacaa
     2581 attggagcaa gaaacttcat caagatcgtt tcaaatttgt agagaagtat aaagaaatat
     2641 tagaagagta tttgaggagg acagcaaatg ataaaaaaac tcgttcaatt cagcatggat
     2701 ttatatgata tcgaatcagg agcaacacta tctgtggaat cggaccatct aatcataaat
     2761 tttggtggaa agcgccagat tattttgtgg gtagttgatg atgtactgtt tccagaaatt
     2821 gttcatgatt tcgaagaatc aaaagcggtt gagtttgaaa tagtgaaaaa agtaatggaa
     2881 ttgattgaaa aatacgagga ggacagcgaa tgataccgaa gtttagagcg tgggataaac
     2941 gaaagaacgt aatgagagat gtagccgtct tgcattttac taaaaacggc aaaacaaact
     3001 ttattgaata ttggataaat cctaccgaat tgaaatcata tcacgtgcga aacatcgacc
     3061 tcatgcaatc cacaggaatg aaagataaga atggtgtgga tatatttgaa ggggatatag
     3121 tattagtcag cgtgcgaaat ggcttcgatt acttagataa taaagtctgt attgtcaaaa
     3181 attcaataga ttattccgga ttagtttgtg ccactgttga tgaagactta gagtatcaaa
     3241 tttttaacac agagctgttt gaagaataca cgtacgaagt caccggaaat atatacgaga
     3301 atagcgagtt attggaggaa cagcgatgaa taaacaggaa gcaatcaaaa agttagaaag
     3361 tattaaagcg ataggaaatg atgcaatagc tgcttgctat aacgagagta taaattcagg
     3421 tattacgtta atgaaaaaaa tagacgaacc gcagaaacca gttattccac aactcgtggc
     3481 cggttggctt gagaaatcta cggacccttt tacaaaagct gaaaaaatag cgtatttaat
     3541 caaatctaaa gatggtgatt catattattt ctgtgattgg tttgtacgag atggcatagt
     3601 gacgcaagag caaggagaag aattactcgc ttgggcaaca agacaatcat atgaaacact
     3661 attgagccta tacaacggct acgaggttga gaaagagccg ttatgggcaa taaagaatgc
     3721 cgatggaaac tatcttacta aatgtgcttt atggggaaaa gatggagtaa attatagttt
     3781 tgaatgcaat ccatctcatc gattgctttt cactgataaa gcaacagcgg atgctgcagc
     3841 attgttggtg aatggaacag tggaagaggt ggtagaaaga tgaaactaaa agacggattt
     3901 tacgctagca gtcacggtat cggcggttta atgctagata tgccgacaaa gaaccctaaa
     3961 acacgcaaga aatcaaaatt caaagtcggt gacatggtcc gctgtgaagc agaagagttc
     4021 gtttatccgt tcagaggata cgttaaaaag atactgtcaa actcagcaat cattcgtatt
     4081 gaaaatacaa tgaagtgtga tcgttcgact gcaaaacaca agcattattt agctgtagcg
     4141 agattagttg acatggaagt aatcaagagc aaataaaaaa agccggatcg ctccgactga
     4201 ttcaataaat ccaacacatt tattatatca cataaaggag cggtttgact tgatgcaatt
     4261 gttacgagag gtagatttca aacagacaag atgtaatgcg agagatgtgc tgaagaactt
     4321 tcggcgtttg gagcggatgg caggtcgctc tttgatagat attaagtcgc cgattattac
     4381 ggatatgccg aaggcaccaa agcacggcaa taaggcagaa gacgcgatca ttcagatgat
     4441 ggatatagaa gcggagagag atgcgattct agcggctttg atggctctta gtctaattag
     4501 tcgccagata ctctactaca gcttctgtgt gccagatagc ttctcaaact acaggattag
     4561 ccgtgaagtg ggttattcag aaagaagtat acaacggatg aagtcggaag ctctaataga
     4621 gtttgcagaa gcatataaac acggaagaat aattgcttat aaataatttg gcggtttttt
     4681 ggcggaatga tggcggtttt tagctattta ccagtgatat tatggtagtg tcgaaagatt
     4741 agtgataggt ctaagacaaa ataaaatgta agggaggaaa tctccctcat cgtttaatta
     4801 agcttcgata gacagcagca aataaactaa aggatgtggg ggtcagctcc tacagttagt
     4861 tcatgtgttg ctgtctatta attgcaactc tttcggtttt attgagtatt tactcaactt
     4921 aaaaaaactg ttgtttgtag atgtaagctg ttcctatttg tacata
//
LOCUS       DAYYCY010000166         4960 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000166 DAYYCY010000000
VERSION     DAYYCY010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4960
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.167"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(364..738)
                     /locus_tag="ACHAZB_002415"
     CDS             complement(364..738)
                     /locus_tag="ACHAZB_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8615038.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
     gene            complement(761..2161)
                     /locus_tag="ACHAZB_002416"
     CDS             complement(761..2161)
                     /locus_tag="ACHAZB_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HFM8615039.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            complement(2165..2575)
                     /locus_tag="ACHAZB_002417"
     CDS             complement(2165..2575)
                     /locus_tag="ACHAZB_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8615040.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            complement(2593..3084)
                     /locus_tag="ACHAZB_002418"
     CDS             complement(2593..3084)
                     /locus_tag="ACHAZB_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFM8615041.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            complement(3094..3885)
                     /locus_tag="ACHAZB_002419"
     CDS             complement(3094..3885)
                     /locus_tag="ACHAZB_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8615042.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            complement(3878..4651)
                     /locus_tag="ACHAZB_002420"
     CDS             complement(3878..4651)
                     /locus_tag="ACHAZB_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM8615043.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            complement(4807..>4960)
                     /locus_tag="ACHAZB_002421"
     CDS             complement(4807..>4960)
                     /locus_tag="ACHAZB_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-54"
                     /protein_id="HFM8615044.1"
                     /translation="NEHLKEGAQLFDTFLNVSKDFFSEIKDIYCIEIPKGEYELIYTI
                     FEQTIF"
BASE COUNT         1575 a         1011 c          653 g         1721 t
ORIGIN      
        1 aataaaaaaa ggcatacaga caataaactg tctatacgcc tcacagactc tattctatac
       61 atctaatttt ggctgtcctg caaaaaatat gtgttagcct ccatcgaaag atcaaacttt
      121 ggaaaacatc attataactt tgcttggtaa gagctttgct aaaacatgat atgacttgta
      181 tacaaaatgt ggagtgtatt ccaataaata ggaaggatta caaaatttga aaccaaataa
      241 gtaaaaaata ctgtcacttt gaattaattt gaaatagtaa attctcatta aactattgaa
      301 atgctgatac caaaaaatga agtacttttc gttcatacca acttagagca cttcgttatc
      361 tatttatttc ttcatttggc ttatctctat aatctcatga tttggtccgt agaaattaaa
      421 gtaacggatt ccattttccc agaacggtaa ttccataata tttttatcta ataattcata
      481 tcctaaatct tgaattgaat tgaatacctc atctatgtca tccgtatcta atgctagatg
      541 atcaatggct cctctacact cagtagtttt atcttcccat atttctatta tcgttccatt
      601 caactcgaaa aagccgactt tacttcccac attcatttct tcattaatca gactaaaccc
      661 taaggattca taaaagattt ttgaagctcc aaaatctttt gttggaattc caatatgttg
      721 taaaccattt aatttcactt acatcatccc ttcaattgtt ttaaaatcca cattcataat
      781 ttattgaata gtctagttta tctaccttga ttgctccctc cgtaacagtc atacttaatg
      841 ctgttccgac tttatcatac attctagtac ttaaagcaac attatcaata tataaaacag
      901 ctatcgtatc atcaacaatt aactttagtt catattcttt gcctatttcc aaaaatagtg
      961 gtcgttgcag ccccttatca aacatttgag gccaaggata gttcggagtt tttccgaaag
     1021 taagctgatt attaatcaat gaaatacgaa attcgtatcc ttcttgagta tcactatcta
     1081 caaataattt taaagcaaac tcctttgtta acgatgtaat agtcaaagtt gtttcaaata
     1141 gaaaataatc ttcgatttta ggaacaatta ctctttcttt ttttccaaat tctttctcta
     1201 acttaaaact tgaaaatgga gtgatttctt taaaattctc aattattttt tcaggcagtt
     1261 taacaccaag agttttgtct gtcttttgaa taatttgatg tggaacaagt gcaccgcccc
     1321 aatcccagtt attttgatcg ttctcccctt cttttgttgc cacccagcca gttagaaaac
     1381 ggccagtttc attagttccg actgttcttg ctgcataata ggcttttccg tcgaaagctt
     1441 cgtctactgg tgatttccaa tctgaaaata atcctgctcc gactcgatat ttagtctttt
     1501 tatcttcaca atactctgaa aaaagtaaat accacgttcc atcaatttca aatatatcag
     1561 gcatttcaat catgttaaat tcattattag cccagtagtc tcctttgaaa ttccaatctt
     1621 ttaaatcttc ggattcaaaa tagaccaatc tccccgtcgg ctttttctta cttgctggtt
     1681 ttctagctcc tagtatcaaa acgtattttc catgatcttg atcaaaaatt gcaaaaggat
     1741 cgcgccaatc attcttatca tatccttctt gaggaacaag ttttgctgct tcgccatcct
     1801 ttttccaatg aattaaatct ttagaacttg caatcattaa cacttctgaa gtttttccat
     1861 ccaacttagc tttgcgatta tgtccagtat acaaagctaa atactcattg attccttgga
     1921 caatacttcc agcgtaaatg tattgatcaa tgtcatcgtt gccaccacga tgaattactt
     1981 ccccgtattc ttcaaatttc acaaaatcag tggttgtaac tagtgaccac ccaaaaggat
     2041 cagttaaagg tttaggattt ctcgtatctc gttgatgaaa tagataaaaa gttccatctt
     2101 tataaaaagg catgcaatcc ccaaaccaaa aatctttagg cttgtaaaaa attttttgca
     2161 tttatcactc atcctcttcg ttagtatgtc tttgcatatc tgcaagacac gcaattgtat
     2221 tcatatttct tagattttcg atgatatcat cgcttttttt tccttgtaat ctcaaattga
     2281 cagcctcaat caaaattcct aaattcatgc caccaagaag ttgaaaccca ttttgaaata
     2341 cttcacttct tttagcaaat tcgttgaaag gtgttccacc aatcaagtca gcaaatacca
     2401 taacttcttg attttttact tgagaaacta ttttttcaat gttttcacgg aattcttcaa
     2461 tgcctgcatc tccaagtgtt accacatgac aatttaaatt atcgccaaaa aacattttta
     2521 atgtatctac aaatccaatg gcaatatttc catgagatgt aattataaac tccatccact
     2581 catcctctgt tcttaataaa taaaatcctt taaaaattct tttgaatctc taggagtttg
     2641 tatcactgtc acttcaactc catcactaat tagttcttta aaagcctctc tctcttcttc
     2701 agtaatattt atcgtcggac ggatgcgttc tgttccattc cttcttgata aatttcctac
     2761 gtttacactt gttattggta aaccgttatc aatcaaaaat ttaacatcga ttggagaaac
     2821 tgcaacaata agaacttttt gtccttcata tttaccagaa agaatatttt taactgctac
     2881 ttcccttgta attattgaag ttctaacatt aggtggtgca gcgaggcgaa ctacagcttt
     2941 ttttacgtca tcattggcta cttcattatt aataaccata attctgttag cactcaattg
     3001 atttgtccat tgtgttgcta cttgtccatg aatcattcga tcatcaatac gaacatgtat
     3061 aatacctttc attttttcat ccatattcaa cacctacttc aaaatattta acgctcttaa
     3121 cgcaatagaa actactaaga ttaatagaat tacataaact ggtttaacct ttcgtcctaa
     3181 caaataatac acaattgcta ctacacatgc aggtaataaa ccaggcataa cttgatcaaa
     3241 gatttcattt ccggtcattg ttaagtcgcc ttgtttaaat tcaaagccaa cattcacttt
     3301 tacaactgtg gcaatgagtg caccgaccac cattaaccct aaaattgata ctgcatcagt
     3361 aatagacttt aattttgttt caatttgagt tagtaggctc gcacccgatt ctttcccagc
     3421 aaggtataaa ggataagcag taatccttaa taaaattgat gccagtatcc acaaaataat
     3481 tccaacaaaa ctaccttcca atgccatatt agctgcaatt gctccaaaaa tagtccatgg
     3541 aataactaca aatacactat ctccaattcc cgctaaagga cccattagac ctgttttcag
     3601 agcacttact gcttccaaag acttattttt ttcttctttt tcaattccaa cattcatacc
     3661 aataatgtaa ggcgctgtat acggcgtagt gttaaaaaat tgtaagtgag ctaaaacgga
     3721 ttgtttcatt ccttcaggat catctttata tgtgtccttg ataaaaggaa gcatagaata
     3781 cgcataaccc aacccttcca ttttttcata atttaatgaa gttgcaccat tgagaatcca
     3841 ccggaaaatt acttgtttgt cgatttttgt cagtttctta ttcatcttcg tcaaaacctc
     3901 ctgttgcagc tggtgctact tgagtattat ttgatctatt tttatatacg attagtgcga
     3961 ttgcagcgcc aagtaacgaa atccctaaaa ttggtatttg gaaataaact gccaaaacga
     4021 aaccaactaa caaataggca aagtaatctt ttgtaggtaa atatttaagt agcattccta
     4081 tcccaacagc aggtaatagt ccccctgcta cctttaaacc accggtaacc cattcaggaa
     4141 tataattgat tgcacttccc actaacgttg ggcctaatat cacagttaac aaaacaggaa
     4201 ttccagatac aaagcttgta ttaattgatg ccaaatattg catccactct aatttttgat
     4261 aatcttcttt ttgtgcgaat ttttctgcct tttgttgaaa aaaaatgttt gttgaccact
     4321 ttagaacatc tagttgtaca attaacattg ctaccggtat tgctaaagtt attcctatag
     4381 aagcatcctg gttagttgag attgctaaat aagtaccgat caatgctgct gtttgataat
     4441 ccggtactga tgcaccacca aaactagtaa tacctagtgt cattaattgc aaagtcccac
     4501 cgatataaag tccagtttga atatctccca taataagtcc ggtcaagaaa cctgcaacga
     4561 caggttggga ggttccgatt ttgggccctt ccttatccca gttaatgaaa aatccataca
     4621 acacaactaa aaatgtttgc caaaacgcca ttttgttctc tcctttcttc ttatactttt
     4681 atataagcaa ttactgtgcc aactaacatt atccaataaa gcgggaaaat acagtaaata
     4741 ttaattaaat gagcgtctaa atatactttg gacacccttt cattttttta gacgctagta
     4801 tgctatttag aaaatagtct gctcaaaaat tgtatagatt aactcatact ctcctttggg
     4861 tatttcaata cagtaaatat ctttgatttc tgaaaaaaaa tcttttgata catttaaaaa
     4921 agtatcaaat agttgcgctc cctctttcaa atgttcattt
//
LOCUS       DAYYCY010000167         4935 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000167 DAYYCY010000000
VERSION     DAYYCY010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4935
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.168"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(350..1234)
                     /gene="rsmA"
                     /locus_tag="ACHAZB_002422"
     CDS             complement(350..1234)
                     /gene="rsmA"
                     /locus_tag="ACHAZB_002422"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFM8615045.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            complement(1227..1796)
                     /gene="rnmV"
                     /locus_tag="ACHAZB_002423"
     CDS             complement(1227..1796)
                     /gene="rnmV"
                     /locus_tag="ACHAZB_002423"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HFM8615046.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            complement(1789..2565)
                     /locus_tag="ACHAZB_002424"
     CDS             complement(1789..2565)
                     /locus_tag="ACHAZB_002424"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFM8615047.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            complement(2720..4042)
                     /locus_tag="ACHAZB_002425"
     CDS             complement(2720..4042)
                     /locus_tag="ACHAZB_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFM8615048.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            4217..4804
                     /locus_tag="ACHAZB_002426"
     CDS             4217..4804
                     /locus_tag="ACHAZB_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8615049.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
BASE COUNT         1404 a         1046 c          861 g         1624 t
ORIGIN      
        1 aatgagtgtg agtatcaagt ataagaattt tttgatcaag aaacaaaaat aggagatgtg
       61 acaaaatttg tgtcacatct ccttgttcgg cataatagtg gtcaaaagct tgcatcaagt
      121 aataagacca actaactcaa aaacatgaga agctgttttt tgaaattgtt ccttgtgact
      181 tagagttaaa cggcaggccc acaacctctt tcttaaacgg tgttcaaaag cttgcatctg
      241 cggtaacaag cccaaacaaa cgaaaaacat gaggaactat ttttgtttgg ctgttcttat
      301 tacttgatgc aggacagctt tttcacaact tctgtcacaa cctctttttt tactgcatat
      361 tttctgacat cgcattgctt aatgcggcga attcttgtaa tgacaaagtc tccccacgac
      421 gttttggatc gattccagct gtttccaagc tttttgctaa ccatgctttt gtctggtcat
      481 ctttgccata cgaatgctgc aagttattcc aaagcgtttt tcttctttgc tgaaatgccg
      541 cttttgtcaa tcggaaaaat gctttttcgt ctgtcacttc gactgccggt atatctcttc
      601 ttgtcagttt caaaatcgct gaatctacat ttggctgagg gacaaatact gtttttggta
      661 cgatgaatgc taaactggct tccatataat attgaacagc aatcgataat gaaccataag
      721 ctttcgttcc tggttcagca gagatacgat cagctacttc tttttgcatc atgacgacta
      781 gttcatccac aaccaaatct gattcaagaa aatgcatcat gatcggtgtc gtaatatagt
      841 aaggtaaatt cgctacgact tttaacggaa gctcctcatg aaatgtttct ctaacagctg
      901 ttgacaaatc ggtctttaaa atatcttgat gtacaatagt cacattgtca tagggttgca
      961 tcgtatcagc caatacaggg atcaaacgat catcgatctc aaatgctacg acttgttttg
     1021 catgcttggc taattgttcc gtcaatgcac cgattcctgg tccaacttcg atcacgttcg
     1081 tatgttcatc tatttgcgca gtttctacga ttttccttaa aatatttggt tcagtcagaa
     1141 aattttgtcc taaacttttt ttaaatgaaa aaccatgttt caataaaatc tcttttgttc
     1201 tagagggtgt cgcaatttca cgatattcac tcatgttgtt cctccactat tttcattgct
     1261 tgggcgagtt ctttcggcgt aatacgaaac atcttcaaac gtttttccaa ttgtttgccg
     1321 ttcgtgtaac cgatgcgcaa ctcgtcacct agtttttctc gtttttcttt tgcttgtgcc
     1381 ccagctatta aaccatagtc taaaaggtct tgtctagtga tttcagaaac agccgtttgg
     1441 gaaatgtctg gcgtcacgac ctttttcaat gcttctaaaa tagcagcatc acttgcatgc
     1501 tcgacaccaa ggcttccctt tttctttggt ttagcttgtg tgcgcggcaa aaaagcatgt
     1561 tttgcctgag gaacagcttc cataatgatt tttcttattt tttctcctga aaaatcagga
     1621 tcggtaaaaa cgatcactcc acgagtctct tgggcatgtt caatttgcat caggatctct
     1681 tcattgattg cagaccctat tgtttctatc gtatctgcat caactacttc ccgcagcctt
     1741 cttgtatcgt cttttccttc aacgactata atctcttgga tcttcatctt atccatgttc
     1801 gcttagacca aataatcggt gcgcatttgt ccatgtttgg gcagccactt cttctgtcgg
     1861 aagattccga agttcagcaa tcttttctac tgtataacga gtatagcctg gttcatttcg
     1921 ctttcctcta aaaggaactg gtgccagata aggggcatct gtttccacaa gtaatcgttc
     1981 gattggtaca tttgctgcag cctcttggac gtctaatgct tttttgaaag tgacgacacc
     2041 gctaaatgaa atatgcatcc ctaagtcaag aaatcgtttt gcccattcat aatcaccact
     2101 gaaactatgc atgattccac cgatatctcg aacatcttct tcttttagga tttgatacgt
     2161 gtcttcaagt gcttctctag tatgaatact gattggtagg ttcatttctt ttgcgatagc
     2221 aatctgtctg cgaaatactt tatcttggac ttctttcggg tcttccatcc aatgataatc
     2281 caagccaatt tcccctaatg ctacaacctt tggtaaagtc aattgttctt gtaatttatt
     2341 ttcgatttct ttcgtataac tgccagcctc tgtcggatgc cagccgataa tactgtatat
     2401 atttggatag tcatggctta attgcaaaga cttttcaatc gttggcgtat cgaagcccac
     2461 gaccgccatt ttcgttaccc ccatttcttg tgctcgctga atcgtttctg gaatatcttc
     2521 attgaactgt tcagcattta aatgggtatg agaatcaaat atcatgttta tctctccttt
     2581 tcattcttac tttagcacat aagcactact tgcagaagta gcaaaaatca aaatagaaga
     2641 acttgtttta ttgaaataga aaaagcacga agatctagag gacctagttc gtactttttc
     2701 tgtctgttgt tgaaatattt tactcttctg agcgtttttt ctttttttga taatagactg
     2761 ggatacctat caaggtaaca cctataccga tcaatgctaa tttcgtttgc gtcatcatcg
     2821 tcatacctaa aatgaaaata gcaccgagaa tcgcaataat cggaacgatt ggataaagtg
     2881 gtactttata tggacgtggc aattccggcg ctttttttct taaaataaag acaccgataa
     2941 aaatcagtaa actgaatagc cacatcacaa aaatcaacat gtctgttaaa aagtcgaaag
     3001 aaccaaggct catcataata caagctactg ctaactggaa aaccgcagga acataaggaa
     3061 ccgtaaactt ctttgaaagc tttgtcagct gtttactgaa aggtagctcg tcttctaaag
     3121 ccatcgcata aggcacgcgg atcccagtta gtgtataacc gttcaatgca ccgtataccg
     3181 agatcaagat cccaatagtg accagctttc caccgattcc tccaaaaatg acgtctgatg
     3241 cttcagaagc tgcattcaaa ttaccggcaa gatgatcaat cggcaaagtt ttcaaaaaaa
     3301 caaaattgat caatagataa acgactgtta caaaactcaa accaagaata attgctttag
     3361 gcaaatcttt ttccggccgt ttcatttccc ctgccattgc accgacacct aaccagccgt
     3421 cataagcaaa caacgttgct aacaacgcac tgctcaatcc ttcgacaaaa gtaacatctt
     3481 ttccagcttc gattggaaat aactggacag ttccttgtcc tggggtcaat aatccccata
     3541 tgacaatcac agcaattggt attaatttaa caatcaacgt cactgattgt acagaagcac
     3601 cgacttttgt ccctaacaaa ttcaaccctg tcacagaaac agcggtgatc actgcaatca
     3661 acaaaagata atcggtagat aattgtaata agttcgtcaa ttgtgtcgca aagataatcg
     3721 ataatgcagc aatattagca ggaaagtaaa tcaagctttg cgcccatcct aataaaaagc
     3781 tgggcaattt tccatacgca gcttcaatat attttacagc tccaccagtt tccgggattg
     3841 ctgtagctaa ttcagccact gtcaatccag cacagatcgt taagaaacca cccaaaagcc
     3901 aagctagcaa tgtcaaactt gtagactgag tactagcagt aacagcagca gttttgaaga
     3961 agacgcctgc accgatcacg gttcccataa cggtagctaa tgcgccaaac agattgattt
     4021 ctcgttttag tttgcctttc attcccatcc tctttcttct ttgaaatttg gtataatttt
     4081 cagtataact gagaatattt taatttacca ttcttttttc tccactttca gtgaaaaact
     4141 ccctcttaag acggatgtat ttctttttta tttttcgtat actaaaccta ccttttccac
     4201 gaaggaggaa cagtttatgg attttaagcg ttcttatccg aaattagcag attatttatc
     4261 caaccgggtc gatttttccc gtaaagaaca acagcagtcc cattggcatt ggttcacatt
     4321 aattttattt ggattaatga ctgttgcaac gttatcacaa aaactaatat tgctgacttt
     4381 gttcatcgca atcgttgcat tgatcaaagg tcctgtgatg atcttgtggg ggatgttgta
     4441 ttcctttttg gttgggcttt ttccgcctat cggtattttg ctttcagcga ttttcttttt
     4501 attgaatatc ggaacattta caaagaactg gcggatgacc ttagtcggga tctattttta
     4561 tttctatcct tttggagtga tggcacttag tgaagtcatg cattgggata atcattggtt
     4621 tatagctggc tccttgcttt tagggttgat cttgttacac gtgatgttga caaagctcta
     4681 tcaacattat ggtatcggac gtacgatttt ctggtatgtc ttttcgatcc catttgcact
     4741 gcttactgct ctcttaccaa gccgtttgaa gacaaaaata aaaggttacc ataaaatcaa
     4801 gtgataggat ttttacaaaa taaagaaaac aatcatcgaa gtcaaagccg atgattgttt
     4861 ttttatctat attttcaatc acacatataa gagcacaaac taattgaaca taaagtgata
     4921 gaacttataa ttaac
//
LOCUS       DAYYCY010000168         4788 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000168 DAYYCY010000000
VERSION     DAYYCY010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4788
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.169"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            564..989
                     /gene="bacA"
                     /locus_tag="ACHAZB_002427"
     CDS             564..989
                     /gene="bacA"
                     /locus_tag="ACHAZB_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin 51 precursor BacA"
                     /protein_id="HFM8615050.1"
                     /translation="MICKNKKKIIAIIASFSLIFSTACTVLLTNNANVEAASSSYKHN
                     HAGYRCYKMSYYVSPSKCKQLARQTNKLNTVSAIANFIGLSGLTPGIISLVFGSAVSA
                     NNVFVTAARRGKGVQLNYIAHFSNTTTYNYSSNQNYVIK"
     gene            1019..1183
                     /locus_tag="ACHAZB_002428"
     CDS             1019..1183
                     /locus_tag="ACHAZB_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8615051.1"
                     /translation="MKLTKIAVILAVIAGIILYFVLPANQFFLYFGIPAILIVNGVRI
                     FEQRKKCSST"
     gene            2141..3118
                     /locus_tag="ACHAZB_002429"
     CDS             2141..3118
                     /locus_tag="ACHAZB_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFM8615052.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGIA
                     YFSKEDFHELLDIPKSYRESDINKKIIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEEQNKALELVNQQIYSEKDPTSLQEKIKEQKRKLELL
                     QQLEELERKQNNLNKENKEINEQIPDNNSDIPPELKKGLLDNLQNLFKE"
     gene            3128..3625
                     /locus_tag="ACHAZB_002430"
     CDS             3128..3625
                     /locus_tag="ACHAZB_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFM8615053.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNIIYLKKE
                     AVTTITKVLSGNEPGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQLALETQ
                     QKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIEKQSKTEKKW
                     WQFWQ"
     gene            4001..4282
                     /locus_tag="ACHAZB_002431"
     CDS             4001..4282
                     /locus_tag="ACHAZB_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000353845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8615054.1"
                     /translation="MDILVRNINPKIITRIDKRCQELTTKTGKKWSRNQYLKILIEND
                     FDHELMNYKKDQFDLLFEKFIAIQAENTNILNDYVHTNNQLIETLIGGI"
     gene            4284..4700
                     /locus_tag="ACHAZB_002432"
     CDS             4284..4700
                     /locus_tag="ACHAZB_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8615055.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPATNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
BASE COUNT         1758 a          817 c          785 g         1428 t
ORIGIN      
        1 aaaaaaaaaa gaaaaatccg cttctgcgaa ttcttctttt ttcattttgg ggcgctgccc
       61 taatccctaa gtcccggtta ttcaatagca tggaaactca aagaataaga cgttccctgg
      121 aataaaaaaa aggctgctcg ttcgagcagt ccatacatag gaaaatgaaa aaacgacgtc
      181 ccttactgag aaacgctatt aaacttttaa caaatcctct aaaaaataac aagtctttaa
      241 aaagtgaccg gtaaaaatgc acaaagaact aaaaaagaga aattgcatca aatcaagcat
      301 tagaagctag taactagcta gaccaattga aaatcacaaa gtgagcgcct gtatctattg
      361 ctacaggcgc tacttggcat agtacacatt atgtcaacaa gaatttaata taacatattt
      421 ttctagtttg ttataacaat gccactctaa caaaaatgta agcattatcc ttattaaaag
      481 ctccttgaca aggactatac cttttatttt atgattttta catagaaaac gttttctcta
      541 ttttttgttt agaggtgatt tttatgattt gcaagaacaa gaaaaaaatt attgctatta
      601 ttgctagttt ctctttgatt tttagtactg catgtactgt cttattaaca aataatgcta
      661 atgttgaagc tgcaagctct tcttacaaac ataatcatgc tggttataga tgttacaaaa
      721 tgagttatta tgtaagtcct tctaaatgca aacaattggc tcgccaaact aataaactta
      781 ataccgtctc agctattgca aactttattg gactatctgg attaacaccg gggattatat
      841 cattagtttt cggatcagca gttagtgcta ataatgtatt tgttacagcc gctagaagag
      901 gaaaaggcgt tcaattaaat tatattgccc attttagtaa taccactacc tataattata
      961 gttctaacca aaattatgtt ataaaataat taaaacttta aactaggagg aatgctttat
     1021 gaaattaaca aaaattgctg taattttagc tgttattgct ggcattattt tatattttgt
     1081 attgcctgct aatcaattct tcctttactt tggaatacct gcgattttaa tagtaaatgg
     1141 cgtaagaatc tttgaacaac gaaaaaaatg tagctctacc taatcgttac aaaacaacaa
     1201 tttcaccttc ggtgaaataa gcgaaacttt acaaaaagtg gctagcaaat gctagccttt
     1261 tttgtattgt agagcgtgaa ttttgagggg gattcaaggg ggagcttgcg cccctttgac
     1321 aagcggttat ttgcaccaaa tacctccgta tttggctagc aatccttcta aaaaatacac
     1381 ctatcgatca gagtcaaaac aaatcaattt tcgagtgagc gaatccgaat ttgcaaagct
     1441 gaagctttgc aaatgagtgt gccggcttcc gtaaaaatac atatggctat aatttgcgtt
     1501 ttaaggcgtt ttaaacgttc tggtatataa ttataccaaa acaactttaa aacgccttag
     1561 atctaatttt agaggtctta tagaacattt tagggttgtt agggcttgcc ctgaccgcct
     1621 gtaaggcgct ttgagaacaa gataagggtt ttatactaag actgtcttaa aacggcttaa
     1681 acaaacgctc agaacttttc aaaggagttc tttatgaact gtttttaagc aaaggagcct
     1741 ttaggcgact gcgcagtata atgtgagagc gtagcgatca cacattttta gtgaccgtag
     1801 ggagcgagtt tcccagttta gaaaaattcg cgaacttgtt tgaggaaaaa agcaggagtt
     1861 ttttaggcgg gaaattttgc ctcctctttt ataatctctt tttaaacctc tttttaaacc
     1921 tctttaccgt cggctgtatg cttactctcc caaggtgtgc agaatctttt gggggtcttt
     1981 ttgtctgttt ttaggggtct ttttgtctgt ttttaggggt ctttttgtct gtttttgggg
     2041 gtctttttgt ctgtttttag gggtctttaa ttaggggctt gacccctatt agaaagctct
     2101 ttataatttt gttaagtagt taatcttgaa ggaggatttt atggcaaatg aaattgtgaa
     2161 ataccaccat gaattaaata cgattccttt acgcaaattt acgtcagtag aaatgaattt
     2221 attcttctct atagtatcta gaatgcgtga cgtaggcgat aaaaaagtac aatttacatt
     2281 cgaacaatta aaagatttaa gcaattataa agcaactgca aatgttcgat tcatagatga
     2341 tttagaaaca acgtatgaca aattaatgga tttacgtttt ggtagaagaa gtgctgacgg
     2401 tttgcaacgt gaacgatttg ttttatttaa tcaatttaaa atcgatggaa aagctgatat
     2461 tccttttgct gaaatacaag tccatgagaa agccttaccc ttacttaata atcttgaaga
     2521 atgggttcga tattctctcc aacaattcaa tgaattagaa agcagttatt ccaaaaccat
     2581 gtttagatta ttaaaacaat acagaaccaa aggaattgcc tatttttcaa aagaagactt
     2641 tcatgaatta cttgatatcc ctaaaagtta tagagaaagt gatataaata aaaaaattat
     2701 aaagcctatt cgccaagaac taaccgcaat cttcaaaggt ctgacaatca aaaagaaata
     2761 cggaaaagga agaggaaaac ctgtcattgg ctatcaattc acgtttaaac ctgaaataaa
     2821 aaaagcagac gactttaata aacacgcacc aacaaaagaa gaacaaaata aagctttaga
     2881 actagttaat caacaaattt actctgaaaa agaccccact tcattacaag aaaaaatcaa
     2941 agaacagaaa cgaaaattag aacttttaca acaattagaa gagctagaaa gaaaacaaaa
     3001 taatctgaat aaagaaaata aggagattaa cgaacaaata ccagataaca attccgatat
     3061 tcccccagaa ttaaaaaaag gcttactaga taatcttcaa aacttattta aagaataagg
     3121 ggtttttatg acagataaaa ctatcaaaga attagcagat attctaggtg tttcaaaaga
     3181 taaagtgaag tatcaactga ggaaattacc tagtaatttt acctacaaaa aaggaaatat
     3241 tatctacctg aaaaaagaag cagtaacaac aattacaaag gttttatcag gtaatgagcc
     3301 gggtaactta cctagtgaat tacctgattc attacctacc gattacctac tcaaggagat
     3361 tgaaactaag aataatcata ttgaagaaaa agataggcaa attgaaaaac ttcaactagc
     3421 tttagaaaca caacaaaaat tactcgacca gcaacaacaa ctcaccctgc aagccaacca
     3481 acaaatcgag aaactgcagg aacaactaca gttgacttat acggaagaat cttcggaaaa
     3541 taaatcaacg actctttctg aagaaacaga atcgatagaa aaacaatcaa agacggagaa
     3601 aaaatggtgg caattctggc aataaaacca gcaatttaaa ttaataaaaa ttgctggttt
     3661 tattcttctt tttgcttttg cgagcagagc aacatgggcc catgttgcga tattttggtg
     3721 gcgccccaat ccccgttttt tcgttgaatc ttactcggac tgcactcgtt ttacagctga
     3781 tttttctgaa ttttgaaaga gcataaggaa acgtggtatc actttcttac gaaaggtgct
     3841 agataagctg tgcctatcac gtttctcaga aaaaaatttg ctatcacttt tttgccattg
     3901 tggctaggct atgtatagtg ttttggcgaa aaatagctat acaaatacta tatttgtatg
     3961 atatacataa attatatact gatacgaaag gaaaatttcc atggacattt tagttcgaaa
     4021 tatcaatccc aaaattatta caagaataga taaacgttgt caggaactaa caaccaaaac
     4081 gggcaaaaaa tggagtcgaa atcaatacct aaaaatttta atcgaaaacg attttgacca
     4141 cgaacttatg aattacaaaa aagatcaatt cgatttatta ttcgaaaaat ttattgccat
     4201 tcaagcagaa aatacgaaca tactaaatga ctatgttcac acaaacaacc aactcattga
     4261 aacacttatc ggaggaatat aacatgagaa aaacttctgt tcaaaaacac gtaacccttt
     4321 atcccagtca cgttgacgtt ttagattcca tcatcgaaaa aaatccagac gtcaacaatt
     4381 attcagaagc cattcgcttt ttatgtatga gttatgaacc tgctaccaat acaaccacaa
     4441 ccagtaacca agcaaaactc aatgccatgg gaaaagaaat ttctatcatg acagaaatca
     4501 tggcagaatt tgctgattta tatctaaaag atactggtgt tcttatcggt acaaattcta
     4561 aagtatatca agaagtgaaa gaaaaaattg aaaatcaact caatccttcc cgacgaaatc
     4621 aacccaaaaa tcgtcaaaaa gaaaccaatc aaacctttac ttctggaaat tttgacccaa
     4681 caaactttct aaaaagataa aaaaagaaac cgaaaaataa aaaaattcct cgatttcttt
     4741 ttagggatcg gtcttccagc aagatttgca agttggtacc aacttgcg
//
LOCUS       DAYYCY010000169         4770 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000169 DAYYCY010000000
VERSION     DAYYCY010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4770
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.170"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            73..789
                     /locus_tag="ACHAZB_002433"
     CDS             73..789
                     /locus_tag="ACHAZB_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFM8615056.1"
                     /translation="MVDNYERKNILKKKSIGCRMKIVAIVPMKLNNKRLPQKNTKAFT
                     NGKPLCYYILSTLLKVKQIDKVYVYCSNPDIKEFIPEGVTFLKRSSSLDQDTTSITQV
                     LAGFTKEVEADVYVMTHTTAPFISEKSIEKGLDAVLTGEYDSAFAAKKLQDFLWKDGE
                     PFNYELDNIPRTQDLPPLYEETSGFYIYNRNVMTELHRRIGKKPCIVEVGEIESVDID
                     EAEDFMIADAIYNHLILQGS"
     gene            808..2391
                     /locus_tag="ACHAZB_002434"
     CDS             808..2391
                     /locus_tag="ACHAZB_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase catalytic domain-containing protein"
                     /protein_id="HFM8615057.1"
                     /translation="MNNVQILDCTLRDGGYCNNWEFGFDNTKKIISGLIESGIEIIEC
                     GFLTNKTVYNKNVTKYTTLEELAQIIPEKREGKLFVAMMNYGEYNIEDLPDYDGSSID
                     GIRIAFHKKNCNEALKLCREIKKKGYLVFVQAMVSLNYTDEEFLAVIKNVNQFKPYAF
                     YIVDSFGMMKGKDLTRLFYMIEHNLEKDIQIGFHSHNNMQLAYSNAQKLITVQTNRSL
                     IIDSSVYGMGRGAGNLNTELFVEYLNDNAGKKYQLKPLLYIIDEILNSFYQKSYWGYS
                     LPNYISAVHNAHPNYASYLSYKNTLTIEAMNEIFEMMDEEKKVSYDKQYIEDLYYKYM
                     ETGKIQEEYMEELRKFLSDKKILLIAPGKSSVDEVERIKEFVTKEEVVIISVNFEYSH
                     IDIDYVFLSNLRRFHELPKESRTKCIVTSNIQASDVYLRTSYKKLLSEREVVRDNAGL
                     MAIRFLADMNVDEIFLAGFDGYSHNEDENYGEQSMEIITKFALLDAMNVQMRDELSEL
                     AKKVKVTFLTDPRWVRIDK"
     gene            2520..3104
                     /locus_tag="ACHAZB_002435"
                     /pseudo
     CDS             2520..3104
                     /locus_tag="ACHAZB_002435"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
     gene            3050..3568
                     /locus_tag="ACHAZB_002436"
     CDS             3050..3568
                     /locus_tag="ACHAZB_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8615058.1"
                     /translation="MLYTEKNDNNIFERHRIIGTIVCITVIPLLISIGILFKFIPNAM
                     VNQMVHIGSPLMVIASIFLFYVFKNFSFYSKYINKLARTSLGVYILHENHYFIDGMKS
                     RGLIWDGIFGLGKRFLDRDFWIWFIIAVVVVCIICMAIDVVREKFERQIFKIKSVKNL
                     CKLMNHVINIYP"
     gene            3591..4616
                     /locus_tag="ACHAZB_002437"
     CDS             3591..4616
                     /locus_tag="ACHAZB_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFM8615059.1"
                     /translation="MKVISFGELLLRLAAPGYSKLFQRDSLETTFCGGEANVAVSLAI
                     FGYQSEFITKLPDSDVGYAAANALRYFGVNVDNIIFGEGRMGLYYLEKGAAQRPSKVI
                     YDRKFSAISLADSDEFKWKEILNDANWFHWTGINPALSENLFETSLDACQAAKKRGIT
                     ISCDLNYRSKLWTSEEAKEKMEQILPLVDICICNEEDAEKVLGIKAEHTNINSGELNK
                     DGYVQVAREIHKKFGCRYVATTLRRSYSASRNGWSAMLYEAEKDKAYFSTEYNIQIVD
                     RVGGGDSFSAGIIYALCENMSGQKAIEFATASSCLKHTIEGDFNRTTIEDVYNLMNSG
                     GNGRVLR"
BASE COUNT         1760 a          527 c          942 g         1541 t
ORIGIN      
        1 tatttcaatg attaagagtc gagagcacaa acggtagacg atgttatttt tataaaaaga
       61 tataatttat taatggtcga taattatgaa aggaaaaata ttcttaaaaa aaagagtata
      121 ggatgtcgta tgaaaatagt cgctatagta ccaatgaagt taaataacaa aagattgcca
      181 caaaaaaaca cgaaagcttt taccaatgga aaaccattgt gttattatat actttcaaca
      241 cttttaaaag taaaacagat tgataaagtg tatgtctatt gtagtaaccc agatattaaa
      301 gaatttatac cagaaggtgt aacattttta aagagatcta gttccttaga tcaggataca
      361 acgagtatta cacaggtatt agccggtttc acaaaagaag tagaggctga tgtatatgta
      421 atgacacaca caacagcacc atttattagc gaaaaatcta ttgaaaaagg actagatgcc
      481 gtgctgacag gagaatatga ttctgcattt gctgcaaaaa aattgcaaga tttcttatgg
      541 aaagacggtg aaccatttaa ttatgaattg gacaatattc ctagaacaca ggatttgccg
      601 cctttatacg aagaaactag tggattttat atttataatc gcaatgtgat gaccgagttg
      661 catagaagaa ttgggaaaaa accttgcata gttgaagtag gtgagattga gtcagttgat
      721 attgatgaag ccgaggattt tatgattgct gatgcgattt ataatcatct tattttacaa
      781 ggtagttaag acagggggaa ggttaggatg aacaatgtac agattttaga ttgcacactt
      841 agagatgggg gctattgtaa caattgggaa tttggatttg ataatacaaa aaaaattatt
      901 tctggactta tagaatcagg aattgagatt atagaatgtg ggtttctcac aaataaaaca
      961 gtatacaata aaaatgttac aaaatatacg accttagaag aattagcaca gataattcct
     1021 gaaaaaagag aaggaaaatt atttgtagca atgatgaact atggcgaata taatatagag
     1081 gatcttccag attatgatgg ttcatctatt gatggaatta ggattgcgtt tcataaaaaa
     1141 aattgcaatg aagcgttgaa attatgtcgt gagataaaga aaaaaggcta cttagttttt
     1201 gttcaagcta tggtatctct caattataca gatgaagagt ttttagcagt aataaaaaat
     1261 gtgaatcagt ttaaaccata cgctttttat attgttgata gttttggaat gatgaaagga
     1321 aaagatttaa ctcgtctttt ttacatgatt gagcataatc tagaaaaaga tatacagata
     1381 ggttttcact cacataataa catgcagctt gcatattcta atgcacaaaa acttataacc
     1441 gtacaaacaa atcgaagcct gattattgat tcttcggtgt acggaatggg aagaggagca
     1501 ggtaatctta acacagaact atttgtagaa tatttgaatg ataatgcagg aaaaaaatat
     1561 caattaaaac cattattata tataattgat gaaatactaa atagtttcta tcagaagagt
     1621 tattggggat attcattacc aaactatatt tcagcagttc ataatgctca tcctaattat
     1681 gcaagctatc tgtcatataa gaatacttta acaattgaag caatgaatga aatattcgaa
     1741 atgatggatg aagaaaaaaa ggtatcttat gataaacaat atatagaaga tttatactac
     1801 aaatatatgg aaactgggaa aatacaggag gaatatatgg aagaactgag aaaatttctc
     1861 tcagacaaaa aaattctact gattgcacca ggaaaaagtt ctgtagatga ggtagaaaga
     1921 ataaaagagt ttgttaccaa agaagaagtc gtaataatta gtgtaaattt tgaatattct
     1981 catatagata tagattacgt atttttaagt aatttaagac gttttcatga attaccgaaa
     2041 gaaagcagaa ctaaatgtat tgttacatca aatatacaag catcggatgt atatttacgg
     2101 acgagttata aaaagttgtt atctgaaagg gaagttgtaa gagacaatgc aggattaatg
     2161 gcaattagat ttttagccga tatgaatgtg gatgaaatat tcttagctgg atttgatgga
     2221 tattcacata atgaagatga gaattatggt gagcaatcca tggaaattat aacaaaattt
     2281 gcattattag atgcaatgaa cgtgcaaatg agagatgaat tgtcagaatt agctaaaaaa
     2341 gtaaaggtga catttttaac ggatccacgt tgggttcgaa ttgataagta gtagtaataa
     2401 tgtttttaaa attttgataa agagatagtg agagaataaa aatgggtaaa gtcttaaata
     2461 aaaaagaaga aaaatattta agggacgatg gggcgagatg aaactctaat tttgaacttt
     2521 taagaatcat ttctatattg tgtattgttg cttaccatgc taatttgcgt ttaaattata
     2581 atgcagacgg aattatagta catggttcat taactgctaa ccagattttc ttgaattata
     2641 ttggtatttg gggtcagcta ggagttaatt gttttatatt tatttcggca tattttttaa
     2701 gtgagaaaag aaacattcac tttgaaaatg ttttgggaat tgtattagaa tgtatttttt
     2761 atagtgtatt agtttatgga attctttgtg catgtggaat agaaacattt tcattgaaca
     2821 gctttgtaaa agaaatatta tcatttgctc taggtagata ttggtttatt acatcctatt
     2881 tagcattgta tattctaagt ccgtatttaa atatcatgct taacaatctg gaaaaacttc
     2941 agttgcaaaa aataagtatg ttattgttta taggtgtatg ctgttatcgt atgttattta
     3001 aaacatcttt gtttggggat tgtgctgttt taattgcact atatgtcata tgttatacac
     3061 tgaaaaaaac gataataata tttttgagag acacagaatc ataggaacaa ttgtatgtat
     3121 aacagttata ccacttctca tatccatagg aatacttttc aaatttatac caaatgcaat
     3181 ggttaaccag atggttcata ttggttctcc attgatggtt atagcaagta tatttttatt
     3241 ttatgtattt aaaaattttt cattttacag taagtatata aataaacttg cgagaaccag
     3301 tttaggggta tatattttac atgaaaatca ttatttcatt gatggtatga agtctagagg
     3361 cttaatatgg gatgggatat ttggattagg caaaaggttt ttagataggg atttttggat
     3421 ttggtttata attgcagttg tagttgtgtg cataatttgc atggctatcg atgttgtaag
     3481 agaaaaattt gaaagacaga tttttaaaat taagagtgta aagaacctgt gtaaacttat
     3541 gaatcatgtt ataaatatat acccataatt aataaatgag gagaaaagaa gtgaaagtta
     3601 tatcattcgg agaattatta ttgagacttg cagcacccgg ttattcaaag ttatttcaaa
     3661 gagatagttt ggagactaca ttctgcggag gggaagctaa tgtagcggta tccttagcaa
     3721 tatttggata tcaatcagaa tttataacaa agcttccgga ttcagatgtt ggatatgcgg
     3781 cagctaatgc tttgcgttat tttggagtaa atgttgataa tattatattt ggagagggac
     3841 gcatgggact gtattattta gaaaaaggag cagctcagag accatctaaa gtaatttatg
     3901 acagaaaatt ttctgcaatt tcgttagctg atagtgatga atttaagtgg aaggagatcc
     3961 taaacgatgc aaattggttt cattggacag ggataaatcc agcattgtct gagaatctct
     4021 ttgagacgtc cttagatgca tgtcaggctg ccaaaaaaag aggtataaca atttcttgtg
     4081 atttaaatta tcgatccaag ctttggacat cagaggaagc aaaagaaaag atggaacaga
     4141 tactaccatt ggttgatatt tgtatttgta atgaagaaga tgcagaaaaa gtgctaggaa
     4201 ttaaagctga gcatacaaat attaatagtg gagaattaaa taaagatggc tatgtgcagg
     4261 tggctaggga gattcacaag aagtttggat gtaggtatgt agctacaact ctgcgtagaa
     4321 gttattcggc aagtagaaat ggttggagtg caatgttata cgaagcagaa aaagataaag
     4381 cgtatttttc cacagaatac aacatacaaa ttgttgatcg cgttggtggt ggagacagct
     4441 tttctgctgg tataatatat gcgttatgtg aaaatatgtc cggacaaaag gctattgaat
     4501 ttgcgactgc atctagttgt ttaaaacata ctatagaagg tgattttaat cgaactacaa
     4561 ttgaggatgt atataattta atgaatagcg gtggaaatgg tcgcgtgttg cgttagtcaa
     4621 catataaaga taaatcacag taatttttat ggtgattatc tgtcttagta gtgattagaa
     4681 taacgtgaaa ataaattgtt gagcgtttat actgttttga aaaataaatt taacatattg
     4741 aaaaatacaa atatatacgt atattataaa
//
LOCUS       DAYYCY010000170         4715 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000170 DAYYCY010000000
VERSION     DAYYCY010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4715
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.171"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            142..1203
                     /locus_tag="ACHAZB_002438"
     CDS             142..1203
                     /locus_tag="ACHAZB_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFM8615060.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            1206..2102
                     /gene="murQ"
                     /locus_tag="ACHAZB_002439"
     CDS             1206..2102
                     /gene="murQ"
                     /locus_tag="ACHAZB_002439"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFM8615061.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            2116..4098
                     /locus_tag="ACHAZB_002440"
     CDS             2116..4098
                     /locus_tag="ACHAZB_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFM8615062.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(4163..4609)
                     /gene="nrdI"
                     /locus_tag="ACHAZB_002441"
     CDS             complement(4163..4609)
                     /gene="nrdI"
                     /locus_tag="ACHAZB_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFM8615063.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
BASE COUNT         1535 a          828 c         1054 g         1298 t
ORIGIN      
        1 ttatgacaat tcttttgata atataaactc aacctttaga aatgaaattt aattatttta
       61 ataaaagtat tgaaattttg tttctttaga tatatactct agtcatagaa atgaagacgg
      121 atacagaaaa agagggataa tatgtttggc ttttcaatat ttatgaacga ttctttgtca
      181 gaagagaaga aacagtatat ccgccaaatg gcatccaatg gctttgtcgg tattttcact
      241 tccatgcata taccagaaga tgatgtttcg gcatatcaaa agcgattgat cgaactagga
      301 agcgaagcga aaaagaataa actagaattg atggtcgata tttcaggaga ggcattggaa
      361 cgagcgggtt tttcattaga agatctaacg cctttgaaag caattggtgt aacaggttta
      421 agaatggatt atcatatttc caatcagcag attgccgact ggtcacatca attgaaaatc
      481 agtttgaatg ctagcacgat cacgcctaaa gatatcgatg aattgaaaga agcagaagct
      541 gatttctcac agattgaagc ttggcataat tattatccac gtcccgaaac agggctggac
      601 aaagaatggt atcagaaaaa aaatcaatgg ctcaaaattc aaggtctcct tgtccaaggt
      661 tttgtaccag gagatacaga gttgagaggc ccactatatc aaggattgcc aactttggaa
      721 gaacatcgag ggatccatcc tcttgcaggc gctctagatc tattagcaag taacacggat
      781 attgtttaca tcggagatgc aggtctgtca gaaaacgttc aggagcaatt tgcttctttc
      841 caaaaagaac agacagtctt gctccatacg gaacctgtag atgaagaatt ttacgaatat
      901 atattaggga agcatacgaa tcggcaagac gatgcaagag atgtcatacg aagcgtagat
      961 gcacgttttc gagagattcc gcctatccca gcaagaaata cagctacgcg tatgaaaggg
     1021 tctattactt tagataacga aaagtatttg cgctatatgg gtgagatcca attaaccaaa
     1081 tatgatttgc cagcggatga aaaagtgaat gtagtagcta aagttatcaa agaagagcta
     1141 ccattgatca accaaatcaa agctggtatg aactatcaat ttataagaaa agaaggaaga
     1201 taaaaatgag caaaatcaat ttagagaacc taacaacgga acgcagaaac caagaaactt
     1261 ttggattaga tgagatgagt atttctcaag ccgttcaact aatgaacaaa gaagatcaga
     1321 aagtggccgc tgcggttgaa aaagaactga caagtatcga accagtgatt gctaacacga
     1381 ttgagtcctt taaaaaaggt ggacgtctga tttatatggg agccggaaca agtggaagac
     1441 taggtgtgtt agatgctgct gaatgcgtac cgacattcgg tgtagaaccg gaaatggtca
     1501 taggtctaat tgctggtgga gaagaagcaa tgactgtagc tgtcgaaggg gccgaagatt
     1561 cttatgagct agggcaagaa gatctaatca agctgaattt gacaaaaaat gatatagtta
     1621 ttggtatcgc agctagcggt cgtacaccat atgtgatcgg tggattagat tacgcaaaat
     1681 cgatcggtgc tgtcacagga acgatttctt gcaataaaca agccgaaatc agtaaacacg
     1741 cggatttccc aatcgaagtc gatgcaggtc cagaattttt gacaggctct actcgattga
     1801 agtcaggaac agcacaaaaa cttatcttga atatgatttc tactatcagt atgattggta
     1861 taggtaaagt attcaataac ttgatggtcg atgtcaaacc aacaaatgaa aaactaatcg
     1921 aacgctcaaa acgaatcatc atgcaagcga cagaagctga ttacgaaaca gctgaaaagt
     1981 actttgaaga agcagatcaa aatgttaagc tggctatcgt catgattttg acaaatagtg
     2041 ataaagagga agctcaagca aaattagctg aagcaaacgg atttgtaaaa gggaccttat
     2101 gagggggaaa agaccatggc aaaaaacgaa ctgactttgg aagaactggc aaaacaaatt
     2161 tataaagggg caggcggcat gggcaatgta gaaagcattg tccactgcat gactcgtgtg
     2221 cgtatgaaga tcatcaatga tgatcttgta aatgtctctg aattaaaagc gattccagga
     2281 gtgcttggtg tagtagaaga tgaacagctt caagtcatca tcggacctgg gaaagtgaac
     2341 aaagtagcac agcaaatggt tgatttggca ggcgtgaaat taggagaaaa actttcttct
     2401 gtaagtacag ctacagcaaa ttcatcagca tctggaaaag accgtgtaaa cgaacgggca
     2461 caagaaatga aagcggcaca aaaatcgaaa caaaaaccat ctaaaatcaa atcggtatta
     2521 aaagacattt ctaatatctt tgtgccaatg attcctgcat ttgtcggtac tgggatcgtg
     2581 gcagggattg ccgcagtttt agcaaactta gtcacagccg gaacacttga tgcagcaaca
     2641 tggcaacaat atatcgatgt gatgaacatc ttgaaaaacg ggatgtttag ctacctagtt
     2701 atctatacag gtatcaatgc tgcacaagtt tttggtgcaa caccaactct tggtggagtt
     2761 atcggggccg ttgtgatgct gacagggatg aatccggaag caccactgaa aaacttattt
     2821 actggtgaag ctcttgcagc tggacaaggt gggatcctag gtgtcatctt cgctgtttgg
     2881 ctattgtcaa tcgttgagaa aaaactgcat aaagtagtac ctgatgcggt agatatcatc
     2941 gttacaccaa ctctttcatt gattgccatc ggtctgattg aaattttctt catcatgcct
     3001 ttagcaggtt tcatttctga tggcatggtc ggtgggatca actgggtact tggcgttggt
     3061 ggagcatttg ccggcttcgt tctaggcaca ttattcttgc cgatggttat gttcggactg
     3121 catcaaattt tgactccaat ccatgtgcaa atgattgatg aaactggtcg tacgctattg
     3181 cttccaattc ttgcgatggc tggtgcagga caagtgggtg cagcattggc attatggatt
     3241 cgctgcaaaa aagataaaga attaacagaa atgatcaaag gagctttacc agtcgggatc
     3301 ctaggaatcg gcgaaccatt gatttacgga gtaacattac ctctaggacg cccatttatt
     3361 actgcttgta tcggtggagg tatcggcggg gcagtgatcg gaatgatcgg aaatgtcggg
     3421 gctatcgcta ttggaccttc aggagccgct ttgatcccat tgatctctga cggtaaatgg
     3481 ttaggctaca tccttggatt attggcagct tatgctggtg gcttcgttgc tacattcttc
     3541 ttcggtattc ctaaagaccg tttagaaaaa gaagaattag cgactgaaac agccacagta
     3601 aatgaagtgc ctgaaaaagt agtacctcaa acttcagctg aaagtgtgac tatcctctca
     3661 tcagttgctg acggaacagt tgtttcttta gataaagcaa gtgatccagt ttttgcacaa
     3721 aagatgatgg gtgaaggcta ctttgtagaa ccagaaaacg gacatatcta ttctccagtt
     3781 actggggtag tcagctctgt cttcccaacg aaacacgcta ttgggatcac aacgccaaat
     3841 ggtttagaaa tcttgcttca tatgggaatc aacacagttg acttaggcgg tacgccattt
     3901 gatgtgaaag taacagaagg gcagcaagtg acacctgata cgcttgtagc agaagctgac
     3961 ttagaagcga tcaaagcagc aggcaaagaa acatccatga tcgtcttggt aaccaatatg
     4021 gatcgtgtaa aaaactttgt cttggaaaaa acaggtaaag caaaagcaaa agcacctgtg
     4081 atggatgtca aagcttaata tgatagaaac aatagaaacg aaagaagaaa ctgaaatcta
     4141 tttttcagct tcttcttttt tattatttat tgttttctgc taaaatctga taaattcgtg
     4201 tcacatcaga tggtgttccc cgtaattcat aatctgccaa gaaaggaaag ccaaatgttt
     4261 gtgcatattg tttggctgtt agacaatact gattattgaa gtttttattt ccgctaccga
     4321 ctacaccaag acaaagttta tgattgtctt catgttccag atattcacgc atcgtttcgg
     4381 tcaagatttc tgtatcaccg ttatctaaac catttccgcc gtctaaataa gtaggaacga
     4441 aagtaaaaaa tggttctgtt tctttcgtaa aatcactatt ttcatggatt tcttttaaag
     4501 taacttcagg attgacagga tcttctgcat gcattttttc agcatattct gctagatgtt
     4561 tggcaaaggc acgcgtattt cctgaaatcg atatatataa aatgttcatg gatcatcctc
     4621 caattctttc cttttagtat agcatctttt ttaagttttt tggtggtgta atttggaaag
     4681 ggactaggcg ctttgcttgt ataatgagaa aaaag
//
LOCUS       DAYYCY010000171         4709 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000171 DAYYCY010000000
VERSION     DAYYCY010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4709
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.172"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            72..1118
                     /locus_tag="ACHAZB_002442"
     CDS             72..1118
                     /locus_tag="ACHAZB_002442"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFM8615064.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            1135..2358
                     /locus_tag="ACHAZB_002443"
     CDS             1135..2358
                     /locus_tag="ACHAZB_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFM8615065.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            2447..3334
                     /locus_tag="ACHAZB_002444"
     CDS             2447..3334
                     /locus_tag="ACHAZB_002444"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFM8615066.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            3420..3650
                     /locus_tag="ACHAZB_002445"
     CDS             3420..3650
                     /locus_tag="ACHAZB_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HFM8615067.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(3707..4672)
                     /locus_tag="ACHAZB_002446"
     CDS             complement(3707..4672)
                     /locus_tag="ACHAZB_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFM8615068.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
BASE COUNT         1590 a          786 c          959 g         1374 t
ORIGIN      
        1 aaaaataggg cttaagtttg agtgacagtt tgtaggctat gttatactat ataagtgaag
       61 ggggacgtaa ggtgaaggta ttattatatt ttgaaagtga aaagatgctt gcaaaatctg
      121 gaatcgggcg tgcgcttgat catcaaaaac gtgctctgac ggaagtcggt atctcttata
      181 cactagatga aaaagaagat tatgacatcc tgcacatcaa tacatacgga atcaatagtc
      241 ataatatggt caataaagcc agaagagaag gaaaaaaagt tgtctatcat gcgcattcta
      301 cagaggaaga tttccgaaat tcctttatcg gatcgaacca gctttctcct atcgtaaaaa
      361 aatatctggt aggattgtac caaaaagcgg actatctgat cacgccaaca ccttattcaa
      421 aacagttgtt ggaaagctat ggtatccgtg tgccgattca agctatttcc aatgggatcg
      481 acttagagaa atatcaccct gatccaatga aagaacaaaa gttcagagaa tatttcaaac
      541 tgtctccgga tcaaaaagta atcatttgtg tcggtttgtt tttcgaacgt aaaggaatca
      601 ttgatttcgt agaaattgct aagaagatgc ccgaatacac atttatttgg tttggtcatg
      661 ttcccatgta ctcgattcct cggaacatcc ggaaaattgt aaaagaagat catcctgaaa
      721 atgtcctgtt tcctggttac attcgtggag agatcataga aggtgcgtat tcaggtgcag
      781 atctattctt tttcccttct tatgaagaga cagaagggat cgttgtttta gaagctttag
      841 caagcaaaca aaacgtactt gttcgagata ttccagtgta taatggctgg ttagaagata
      901 gcaagaactg ctatatgggg aagacaaacg aagaatttat ccgactgatc caacagatta
      961 ccaatcaaga actaccgagt acgacacaag ccggttatca aacagcaaaa gagcgaagta
     1021 tcaataaaat cggtgaagaa cttaaaaatg tatacgaaag tgtactgaat gaaaaagtct
     1081 ctagcaaatt aaaaaaagta aatcaggtca aggactagtt tggaggcgaa aaacatgaaa
     1141 atcggctttt ttaccgatac ctactttccg caagtcagcg gagttgctac atcgatcaaa
     1201 acattaaaac aagaattaga aagacatggg catgaagtct atatttttac aacgaccgat
     1261 cctaatgcgg ataagttgga aaaagatgtg attcgaatgc caagtgtacc atttatttct
     1321 ttcaaggatc gaagagttgt tgttagaggc atgtggtatg cttatttggt tgccaaagaa
     1381 ctagaactag atcttattca tacccataca gaattcggag cagggatact tgggaagatg
     1441 gtagctaaga agttgaaaat acctctgatc catacttacc atacgatgta tgaagactac
     1501 cttcattata tcggtaaagg aaaagtgatc aggcaatccc atgtaaaata cttgtctaga
     1561 ttatttgcaa atcatacgac aggtgtagtt tgtccaagtg aacgtgttat tgatacatta
     1621 cgcagctatg gtgtaatagc accgcttcgc gtgattccta ctggtatcga cattgaaaaa
     1681 ttcaaacgtt ctgatatcac tcagcaggat atcagcaaat taagggagtc actaggtctt
     1741 caggaaaacc atttgatgat tctttcttta agcaggattt cttatgagaa aaatatccaa
     1801 gccttgatta atgggtttcc tcaggtcatc tccgcttatc ctaacgccag attagttatc
     1861 gtaggaaaag gaccttatgt agaagagtta gaggaattga tctcagaact gcagttagaa
     1921 gaatttgtgc agttcacagg agaagtggac aacaatgatg tagcacttta ttataaagcg
     1981 gcagattatt ttgtcagtgc ttctacttca gaaacacaag ggcttactta tacagaagcg
     2041 atggcagccg gaacaccttg tgtcgtggaa ggaaatgctt atttgaaccg attgttcgat
     2101 catccttcat tagggaaaac ttttgaaacc gacgaagact ttgcaccagc acttattgat
     2161 tatatttcca gtgaagttcc taaagatcca aaagttttag aagctaaact ttatgatatt
     2221 tcatctacac atttcggcga agctatgatc gaattttatc aagacatggt tgtttatcat
     2281 gaagaagaga tgcgaagaaa agaagaagaa gactcgattg aacgaatcaa aataaaatta
     2341 aattcattta aaagataaaa ttcaaacatt ttttatgaaa aaatccttgt ttctagcgta
     2401 tactagcata gaagcaagga tttttgattt ttaggaggtc gaaaaaatga aacttggttt
     2461 tatcggaaca ggcgtcatgg gaagtgctgt cgcacgccat ctactagaag caggacacga
     2521 agtagctgtc tataatcgga caaaagcaaa agctgatcct ttagtaacag aaggagctat
     2581 ctgggcagat acaccaaaag cggtagcaga acaaagtaat attctcttta caatggtagg
     2641 ttaccccaaa gatgtagaag aaatctacta tggacagtcg gggatttttt ctgcagatat
     2701 ctcaggacat attttagttg atttgactac aagtacacca agcctggcag aaaaaattgc
     2761 aaaaacagca aaagaaaaag gcgcagatgc gttagatgca ccggtatccg gtggtgactt
     2821 aggagcaaag aatggtacac tgacgatcat ggtcggtgga gaagaagccg tttatgatca
     2881 agtactgcct ttattcaaag aatttggtac gacatttaca ttgcatggtt ctgctgggaa
     2941 aggtcagcat acaaaaatgg ctaatcagat catgattgca ggtacgatga caggaatgac
     3001 ggaaatgctt gtctatgcac aaaaaaacgg acttgattta aagaaagtga ttgagacgct
     3061 ttcaggtgga agtgcagcta actggtcttt aagcaattat tctcctcgta ttttaaaaga
     3121 agattacaca cctggttttt ttgtcaaaca ttttatcaaa gatttaaaga ttgcattaga
     3181 agaagcagaa aaaatggatc ttgtattgcc agcaactacc caagcattga aactatatga
     3241 agagttggca gataaaggtt ttgagaatga cggaacccaa gctttgatca aactttggtg
     3301 gcctgaagga aaaataccag aaaaaaaatc ataaaaaacg agttttttta gagaaaatca
     3361 atgcaagtag gctatgcttt tgatacaata taacagaaat aagaaaagga ggacctccga
     3421 tgacacattc acaattagtc gcaatcataa aacgactgga ggctatggtc gagtcagcag
     3481 ataatgaact gcaagttcgc cgttttgaaa aagaaggcgt agagaagtgt acagtaactt
     3541 atgataaagc gaccgataca tttgaattaa ctgaaacaga tactcatcag caatacgaat
     3601 ttgataatat cgatatcgta gcgatggaaa tctatgactt aatccaataa attttaggat
     3661 aattgacatt tgaagttgaa gatgcctaaa agaagccgag aagcaactaa tttagttgct
     3721 tttcagcttc ttttttgttt aacagttcaa tttcaaactg ttctttttct aattttttgc
     3781 ggtaactgat caagatttgt acaggatcga tttcttcttt tgacaattga cgtttcaaag
     3841 ataaatgagc ttctttcttt tcataataga agtcaggaga aacgtcacca gaggtaatca
     3901 ttgtttctgc aagttgacca taacaaacga ggtagtgctt tttctgactc tcatctgctg
     3961 ctaatggcat gctgaaggtg agcgatggtg ttcttaaaaa ctgcttttgt tcctggatcc
     4021 gtctaataaa ataagtaact aattcgatat ctttttgaag cataccggct ttttcgaaat
     4081 tcaactgata gatgaaatgt tgttgtcttt ttttcaagta agtcagtagc tttttatttc
     4141 ggccttgaaa cagcccttgg agttcctttt ttttctgttc aaaagtcatt tcctgtaggt
     4201 caggaagctg gactcgcaaa gctaacaaac taatttcaga caaccaaggc atttgataag
     4261 tttcttccat tatacgcaaa atagacggca tttttttata gagacgaaaa ggcccgtatg
     4321 ttcgagccgt aggagtatct gttaatacaa atcccttatt ggtaatatgt acataattat
     4381 aattactgaa ataattcatt tgtcggttat aaaaaggacg gtaatgttgg atcaattgac
     4441 actcgagcaa taaagcatct aattccgtgt ctactacgac gaaatcaaaa tctgttatat
     4501 gatgaatcat acgtaatact ttttttgaat gctgtttatt ctgatgaaaa tagctgctta
     4561 ctctattttt gagccgtttt gctttaccaa catagatgac cttaccatct cggtctttca
     4621 taagatagac acccggcagc atggggagag ttttcatttt ttcttttaac ataggatctc
     4681 cttttacgct ttgaataaaa agactataa
//
LOCUS       DAYYCY010000172         4682 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000172 DAYYCY010000000
VERSION     DAYYCY010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4682
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.173"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(255..1328)
                     /locus_tag="ACHAZB_002447"
     CDS             complement(255..1328)
                     /locus_tag="ACHAZB_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8615069.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            complement(1325..2152)
                     /locus_tag="ACHAZB_002448"
     CDS             complement(1325..2152)
                     /locus_tag="ACHAZB_002448"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8615070.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            complement(2153..2959)
                     /locus_tag="ACHAZB_002449"
     CDS             complement(2153..2959)
                     /locus_tag="ACHAZB_002449"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8615071.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLLLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            complement(2971..4056)
                     /locus_tag="ACHAZB_002450"
     CDS             complement(2971..4056)
                     /locus_tag="ACHAZB_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8615072.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            complement(4073..4615)
                     /locus_tag="ACHAZB_002451"
     CDS             complement(4073..4615)
                     /locus_tag="ACHAZB_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFM8615073.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
BASE COUNT         1427 a         1016 c          763 g         1476 t
ORIGIN      
        1 ttgcatagtt tccaattact ttatccttct gtaacttttt caatcttaga tttactagcc
       61 cgtctcgggc cctattttac aatggttttg tccacttttc tctggtagaa aaacgtggta
      121 gaaaaaataa aataacacaa aaaaagcggc gtttcatccg cttttggtag aagattggaa
      181 aactcagata gaaaaaggac gatttttagc tctttagaac gttgataagt ccccattctt
      241 actgagtttt gccattatct cctatacatc ttgaattcta agaataggtc attatagatg
      301 cctaggtatt tcgatcctag atctttgtag acttcaagat gggagatcgt gtcatcagat
      361 ggatagaatt gttcgtccga cgaaatgtct tttggcaata acgcttttgc ttcttcattt
      421 ggtgtagaat aaccaatata ctctgcattt tgtgccgcat tttccggttc tagcataaag
      481 ttgatgaaat cataggcacc ttctttattt ttcacggttt ttggcataac gatattatca
      541 aaccaaagat tggatccttc tggtgggatc acataatgta aatgttcatt ttcccacatc
      601 atatcggcag cttcgccaga aaatgtcact gccacggagg cttcttcatt gatcatatac
      661 attttgatct catctgccac gattgctttg acatttggtg ttaaatgatt caatttattt
      721 gctgcttctg tcaactgtgc catattctta ctattcaaag aatagcctaa actattcaaa
      781 gaaagcccca taacttctcg tgcaccgtca atcagcatca aactgtcttt taattccggc
      841 ttccacaaat catcccaatg cttaatttga tttgcatcaa agaatttatc gttatatacg
      901 atccctaaag ttccccaaaa atatggaatc gaatactgat tatctggatc aaaatccaag
      961 tctaaaaatc gtggatcaat attatttaat cctttgatct tcgaatgatc taatggaagt
     1021 actagttttt ctttgatcat tttttggatc atatattcac ttggtatagc gatgtcgtag
     1081 tttgtcccac cttgctggat ttttgtgaac atcgcttcat tagaatcaaa tgtttcataa
     1141 ttcactttgt atccatattc tttttcaaat ttcgtgatca aactaggatc gatataatct
     1201 ccccagttat agatggtaag tacctttgct ccactcatgc cacttgtttt ttcaagttga
     1261 cgaacaccaa gcagcagcac cgcaataatg atgagaatac ctaaaaacag agattgtaat
     1321 tttttcatcg cagccgtgcc accccctcgc gtactcttct gtttttcttc agccgttttg
     1381 gacggttttc tttactaata aagtagtatc caatgaccaa tatcatggag aacaagaaaa
     1441 ctaacgcact tagcgcattg atttctaaac tgattccttg tcttgcacga gaatagatct
     1501 ctactgagag cgtcgtaaag ccatttcctg tgacgaaaaa tgtaaccgca aaatcatcaa
     1561 gagaataggt aaatgccata aaatatccag ctatgattcc tggagaaaga taaggcaaaa
     1621 tgatattttt gactacttgg aaattattag caccaaggtc tctagccgca tcgaccattg
     1681 aatcgttcat ttcctgcaat tttggcaaga ccatcaagac gacgattgga atgctgaaag
     1741 caatatggga aagcaataca ctctgaaaac ctaatccgaa tcctagtagt gtaaagaaaa
     1801 tcaagaaact agcaccgata ataacatcag gagagactaa taagatgtta ttcatgctca
     1861 acaacgctgt ccgtgcacgt cgttttttcg tgtaatagat tcccatagcc ccaaatgtcc
     1921 cgatgatcgt ggcgatcaat gcagataaga aagccagcat gaacgtattc aatacgataa
     1981 tgatcaaacg tgtatcttca aaaacagctt cataattttc ccaagtaaat cctgtaaagc
     2041 tgttcatcgt tcctccttca ttgaaggaat aaaaaatcag atagaagatc ggtgcatata
     2101 agaataaaaa gacgatccct aaataaagat acgaccattt aaattttttc atttatgagc
     2161 gacctccttt tttcttctcg cctgtcagga gcatcacgat gaacatagca atgattaaaa
     2221 tcacaccgat tgtagaaccc atcccccagt tttgtgtaac caagaaatgc tgttcgatag
     2281 ccgttcctaa agtgatcacc cgatttccgc caatcagacg agtcagcatg aataaagaaa
     2341 gagaaggaat aaatactgct tggacaccac ttttcacacc atttaataat aacggaaaaa
     2401 tcactcgtcg gaaggtctca aagctattcg cgcctaaatc tctactagca ctgatcaaag
     2461 aaggattcat ctcttccaat gcattgaaaa ttggcataat cataaatgga atctcgatat
     2521 acgttgccac gaacagaaaa ctgaagtctg tgaataatat ctgtttcggc ccgatcccga
     2581 taaaatcaaa gaaatgattc acgctgccgt gcacactgaa aatcccaata aatgcgtaag
     2641 ctttaagcag cagattcacc catgtaggca aaataatcag catcagccat aattgcttgt
     2701 gcttcagttt tgttaaaaaa taagcagtcg ggtaactgat gatcagcgtg aaaaacgtga
     2761 tcaaaaatgc ataccataca gaattgatcg tcatagaaag cgatgtcccc gacgtaaaat
     2821 aagatagata attattcagc gtgaattgcc cattcatatc aaaaaaagat tgataaatga
     2881 tcattaatac aggcgcgatc acgaataaac cgagccacaa aatataaggg acggaataga
     2941 tcctgcgcat tgttttcatt cttgtccccc ctattcttcg tagctgtcaa gacgtgcatc
     3001 gaattcttct tcagactcgt tgaaacgcat gacatggata tcttctggct caaaggccaa
     3061 tcctactttg cttccttctt tagctttttt cgttgaatgg accatccatt cattttgctg
     3121 ttcatcataa cagatgattt cataatggac accacgaaac agctgagtat cgaccgttac
     3181 gactagtttt cctttatctg gtgtcgtgat cgtcaaatcc tctgggcgta aaacaacttc
     3241 aactggttca tttagacgca tcccgccatc gacacattca aattgcttac caacaaactc
     3301 aactagatta tcttcgatca ttacaccgtc cacgatattg ctctccccaa caaaatcagc
     3361 aacgaaatga ttgattggct catcataaat atctacgggt gtaccacttt ggacgatttt
     3421 ccctttattc atgacaaaaa tctcatcact cattgccaac gcttcttctt ggtcatgagt
     3481 aacaaaaata aacgtgattc ctaatcgttg ttgcagatcc cgcagttcat actgcatatc
     3541 tgtacgtagt ttcaaatcaa gcgccgataa cggttcatct aatagcaata ctttcggttc
     3601 atttacaatt gctcgtgcaa tcgctacacg ctgtctttga ccaccagaca tttcactaat
     3661 ctcccgtttt tcgtatccag gaagctggac catacgcaaa gcatctttta ccttcttttc
     3721 gatatctgat ttggacattt tcttgatctt caatccaaaa gcaacgttat caaaaacatt
     3781 catatgagga aaaagtgcat aatcttggaa gactgtattt acttgtcgct tattagctgg
     3841 caaatcattc aaccgtttcc cttcaaaata gatgtcccct tctgtagctt cggtgaatcc
     3901 agctataata cgtaggatcg ttgttttacc acagccggaa ggaccaagca gtgtataaaa
     3961 ttttccttgt tcgatctcaa aacttacttt ttttaatact gtttcatcat catagcgttt
     4021 cactacttga tcaaatgaaa tgattggttt ccccacgttt ttacccccta tattacaaat
     4081 atgattgcgt tgcaacaatc aatacttttg ttcgtttttt gagatgattc cttaattgat
     4141 gttgttttgt cgcttgataa tagatcgact gtcctgcttt tgccacatag accgtctctc
     4201 ctaagcttaa ctctaattcc ccttctaata caaaaataaa cgtttcagat aatgaaggtt
     4261 caaacgtctt atattctcct gcaccttcaa aagtcagcaa gacaggctcc atttcttttt
     4321 cattcgaatc agccacgagc cattccaatt catacccgtt ttcttcatcg aaataaaccg
     4381 tatgatcttc ttttgaataa accacttgca gattcgctgc ttcttgatga aaaaattctt
     4441 ctggagtcac acctaagact tcaagaatag aaaagaatgt ttccattgat ggtgaactta
     4501 aatctcgttc caactgggaa atataccctt ttgacaaatc tgtgcgttcg cctagttctt
     4561 cttgtgtcaa attcttttgg atgcgcaaat ttcttaactt ctcaccaatt tccataattt
     4621 cctccgaatc gaagttttct aatagtaaac ttcctgttta ctaccactcg tattagtata
     4681 cg
//
LOCUS       DAYYCY010000173         4611 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000173 DAYYCY010000000
VERSION     DAYYCY010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4611
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.174"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(86..400)
                     /locus_tag="ACHAZB_002452"
     CDS             complement(86..400)
                     /locus_tag="ACHAZB_002452"
                     /inference="COORDINATES: protein motif:HMM:NF024673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM8615074.1"
                     /translation="MLCRLLEIPRSVYYFYKNKPLTATEIRNNKLKKKISTIFFTNKQ
                     RYGATKIHQVLLKEGISVSLKHVQKLMKQLNLRSIVVKKYRPQRSNKPIMDRLQLTRQ
                     KN"
     gene            complement(451..705)
                     /locus_tag="ACHAZB_002453"
     CDS             complement(451..705)
                     /locus_tag="ACHAZB_002453"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8615075.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
     gene            complement(898..1173)
                     /gene="prgO"
                     /locus_tag="ACHAZB_002454"
     CDS             complement(898..1173)
                     /gene="prgO"
                     /locus_tag="ACHAZB_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA segregation protein PrgO"
                     /protein_id="HFM8615076.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(1145..2098)
                     /locus_tag="ACHAZB_002455"
     CDS             complement(1145..2098)
                     /locus_tag="ACHAZB_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8615077.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            2710..4203
                     /locus_tag="ACHAZB_002456"
     CDS             2710..4203
                     /locus_tag="ACHAZB_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HFM8615078.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            4338..>4611
                     /locus_tag="ACHAZB_002457"
     CDS             4338..>4611
                     /locus_tag="ACHAZB_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HFM8615079.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELR"
BASE COUNT         1492 a          774 c          802 g         1543 t
ORIGIN      
        1 ttgcttgaat tcttgtgaaa aagttcttcg tgttctctta gacataaaaa ttcctcctgg
       61 tatgttttct tctagtctac acaccttaat ttttctgtct agttaattgt agcctatcca
      121 tgatcggttt attagacctt tgaggtctat attttttaac tacaatcgac cttaaattta
      181 attgtttcat tagcttttgg acatgtttaa gagatactga aatcccttct tttaataaaa
      241 cttgatggat ctttgtggca ccatagcgtt gtttattcgt aaaaaaaatt gttgaaatct
      301 tctttttcaa cttattattt ctgatttctg tagctgtcaa cggtttattc ttataaaaat
      361 aatagacact tctagggatt tctaataacc gacacaacaa agaaacagtg taatccttac
      421 accatttttc aataaattga acaagcgaat ttaggttttc cgagagaata tggctgcggc
      481 tttttttaag atttcaagtt cctcatttaa acgagcattt tcttttctca gttcagctac
      541 ttcttttcca gtcagtcctg tggactgatc aggtaaatat aaattcttcc atttgtaaat
      601 cgttgcttca gataagccat attctttcgc taaccctcga acagaacgtc cagtttgatt
      661 cagttcaaca atcatttgtt taaaattttc ttcatatcgg gtcataaaaa ttcatcctct
      721 cgtagataag tttaatttat tcttgtctct acgaaaagct gtctaagatt tgatactaac
      781 accaaatgtt gtataccccc aaacaataca catattttga aggattagga ttaataaagc
      841 taaaaatata gtaagtatac taaatatatt tactatatct agtatactta ctatatttta
      901 atcttttgat cgtctattat atataatccg ttcctcgtcg tttaaagtat caacataggc
      961 gtccattgca agtttgacta tttcatacat aggaatatct tttgcataac taagatcccg
     1021 aatggttttt agtacgggag gatctacttg aactggtttt ctttctttcg cagtgtactt
     1081 tcgttcccct aactcgacat taatatcatc tttaattgaa atatcttcag caccaatttt
     1141 cttcttatct ttgttcgttg gtaataatgc catttagttt aatctccttt ccaagaggta
     1201 ggatatgatt ggccattgag tcagcatatt taggtgtata atcaaatcct tcaatatcac
     1261 catgttgttc atagtattca attctttctt ctaattctgt aaagatgtct gcaaaaatag
     1321 cccaccatct tcgatctaca taatcattta attggatacc attctcacca tacatttcta
     1381 accgatccga gcctttaaca atgcttttaa aaatgtttga ttcaccatac ttatcaattg
     1441 ttttttgata gtttgcgtgt tgagaggaac gtcgcactga gaaaagaaca ggcaaaattc
     1501 caatgatttg aagatgagac tcttcaaaat tcgtgacaat cggaactaaa actttattaa
     1561 taaaatcttc tgttccttcc attgcgtacc gttttaattc ttgcattgca ataatataat
     1621 ctgcagcagt taagaatgca cgaaccacat tatctgttga tggtgggcag tcgataaaga
     1681 tataatcgaa attttttctc aattcttcta aaggtttaat gaaggccaat gatctcttac
     1741 gacgatcagg atatttttcg gacaagtatt cattaagttc ttcagatcca ggagagccag
     1801 ccataatata taaatgtgga cttacttgag taatacctga aacccagtcc ccttttttga
     1861 ttgctgcaga catggatcga tcaaagctag taatttgata cgttgatcca atgttggaag
     1921 tagtatttct ttgttggtca taatcaatta gcaacacgtc tttttcaaaa agttgactag
     1981 aaacaatcgc taataaatca ttgtctgtag tcttaccaat gccgcctttt ctacctgtaa
     2041 gcattacaac ttttcctagt ttatctgatg ttttaaatgc agtccgtaag ttttccatag
     2101 ttccctccaa tttcctatac taaatatatt tagtatactt catatactta atatattcga
     2161 tatatctaat atactccaaa ttgataagaa ttcagacttt ataaggaata tagtggatat
     2221 actaaatata tttgctatat tcagtatatc agatatattc aaaatgtcaa aatatatttg
     2281 tgcgttttgc gttgacaatt tagagtgaaa agaggtaaac ttaattctat agaaaccaca
     2341 gataattcac tatttacaaa gtagcaaaag cagtggtact ctttaaacag aaaaattaca
     2401 taaaaaaaag agacggaaat aattgatttt agcagaatca attatttctg aatcacatgg
     2461 ttaggacatg tgttcacgct cgtctcaaga atatgtaagt agctaccaac tacttatatt
     2521 aaaagttgat cggatacctt ttccgattgc ctttattcta ccaaaaaaag gcgagcaact
     2581 caacagaaga acgtttattt agtgtactta aattaaatat tcttaaacac tgaagagaga
     2641 acacatacta tggctgagat taaagctaaa ctgtgttctc tttttgtttg agaaaggacg
     2701 ttacaatcga tgttgagcga aatttattca gctattctta aaaacggctt acgtacatac
     2761 aaatatcgaa acagcaccat gaaacctgca ggtcatattg attccagtag gagtggtgct
     2821 atctttgcgt accgtagcaa gcaattaatg aatgttgggc gtggtatggt cattacttcg
     2881 gaagaagcta ttttagagaa cgaaaagaaa ctaacgcatt ggacacctaa tacctatcgc
     2941 tttggtactt atgcggatga ttatcgaaca gtggttaaag gtcattctga gaaaaactta
     3001 agtcaaatca acacatttgt agttgatatc gattcaaaag agaaccatca aggcgaaatt
     3061 attttagcct gtttagatca agtgggctac atgccaacgc taattttaga atcagatcat
     3121 ggttatcaag tttattttgt actaaagaca ccaggctatg tcacaaagaa aagcaatttc
     3181 aaggtgatcg atgtagcaaa aaaaatttca aaaacaattc gtctacagtt ggctgaaaaa
     3241 ttgtccggag tggatctagg atgtaaccac ttcggaattg ctcgattccc acaagaaaat
     3301 aatattgttt ttaaagaaat gaactatcag tattcttttg ctgactggat tcaatggtcg
     3361 atgaagcttg aatcagatca aaaaacaaaa gaagatcgag aacgaaaact aattgtcttt
     3421 cctgagaaaa aagaaattcg tcaagtagat gaaccgtggt tcgatatgtt acttcataaa
     3481 gcaaacatcg tcggcggaga aggacgtctt ggtcgaaata atgtgatttt cacgctttct
     3541 ttagcttatt actcttcagg ttatgcattg gatacttgcg aatacaatat gttcacattc
     3601 aacgaacggt tacaggaacc gctatctgaa gcagaattag taaagattgt acgtagtgct
     3661 tattctggta attatcaagc agcaagcaga gaattcatta ttgaattgtg ccagcaatgg
     3721 gtttcaccaa ccttaaaaaa taatgatctg ttcattcaac gacgtggctg gtggaaattt
     3781 aaaaagccac gagatcaaag aaaatatagt cataaaaatg aatggaaaga agatctattg
     3841 gcttatctaa gcgaaaaaag tcttgctagt gagccttatt tgaacctgtc aaaaaaagaa
     3901 ttggcttcac aactaacgat gcccaagcga acacttgata agatcttaaa tgagttacga
     3961 gaggagaata aagtcttcta caaggtaaca cgaggaagaa atggcggaat gatcattgct
     4021 tcagtcaaaa cgttgttcgc gagggttatt caactaagta aagaaaagag actagattat
     4081 tcaacaagga tcacagaggt atttggactc aacgagagct tagtagaaga gacatttaga
     4141 caattatcaa cccccttgaa ccctcttaaa gaagtgaatc tttttgaagt agatacaggt
     4201 tgaaaaataa aaggtgcaaa ttgccttata caatatatct atgatacttc tgtaggtgtt
     4261 gctccctttg cctagctacg tgtaggtggt atacttataa ctgcgtatgc agtcttatgt
     4321 atcatggagg aagttgtatg tcgttaaaaa attatgtcta ttcacatcct gttaatgtgt
     4381 ttatcattgg tcgtttggga ttatctgtgg aagtgttctg tgagctttat ggttttaagc
     4441 aggggacttt gtcttcttgg gttaccaggg aaaaaacagt tgcttctttg ccgattgagt
     4501 ttcttcatgg gttgtcctta gcttctgggg agactatgga tcaggtatat gattgtttgt
     4561 gtgtgttaga gcaggagtat attgagtata aaatagctaa cgagttaaga a
//
LOCUS       DAYYCY010000174         4591 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000174 DAYYCY010000000
VERSION     DAYYCY010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4591
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.175"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            609..1520
                     /locus_tag="ACHAZB_002458"
     CDS             609..1520
                     /locus_tag="ACHAZB_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="HFM8615080.1"
                     /translation="MKYLLHGYFIDYSTRNSSTLAILCLLHELRVVTRKQLYKLLNFD
                     RSISKQGVTNALVRLYEKQLIDRKKYGTEMYYYLTKEGHQSIGGYYTLPKVPEYNLNH
                     HLQINNYLIKGMALAKDHPHLKRIISERRTVYETKDLNPKTKGRKFFVPDFIFRFRDK
                     ENHEINWSFEIELTLKTRRRYREAIFPKYIRELRTRPESHLIYVTPSGAIQEELHAFK
                     RIFFKRETEKDASQQELFERLHIFSALEFEEGLKALLENDPAVNWRTVEREKIYPNGA
                     VQSRLNLFEESTENEEKPKVKFTPEVL"
     gene            2007..2273
                     /locus_tag="ACHAZB_002459"
     CDS             2007..2273
                     /locus_tag="ACHAZB_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8615081.1"
                     /translation="MTTKNEQLKEQARKELQERGLIIEGSFEGDFETYIGCYARPINK
                     PTALDPMNEQEVKEQEKHAVNGFPQDFTEWYEWEINNGKLENFL"
     gene            2345..2686
                     /locus_tag="ACHAZB_002460"
     CDS             2345..2686
                     /locus_tag="ACHAZB_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8615082.1"
                     /translation="MAKNTPYQVGDIFYSSWGYEQTNVTFWQIVKLTEKTAWFQPIQK
                     KTVEHYSSMSGKVVPMKDKFIEYPLAKTKDSIVKKRIDPETYPDMLCGYRSWDTFHRY
                     KNQAVYESSYY"
     gene            2741..3160
                     /gene="ssb"
                     /locus_tag="ACHAZB_002461"
     CDS             2741..3160
                     /gene="ssb"
                     /locus_tag="ACHAZB_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFM8615083.1"
                     /translation="MMNSVNLVGRLTKEVDLKYTPKGNATGTFILAVNRNYTNANGER
                     EADYIRCVIWRKAAETLAKYTGKGSLIGINGRLQTRSYQNQQGQTMYVTEVLVTDFYL
                     LESKEINEQRSKSMANNQNNSSIPGNEIQISDSYLPF"
     gene            3237..3533
                     /locus_tag="ACHAZB_002462"
     CDS             3237..3533
                     /locus_tag="ACHAZB_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8615084.1"
                     /translation="MKIIDSYDQRTMQYLGSFDYTDENIINYVASLPPYQSVRLVDFA
                     SDELVLTTMGNFLDYVGDKKWLEKILSKLIAKQTGEEPINAIQLFNRYEQENND"
     gene            3548..3979
                     /locus_tag="ACHAZB_002463"
     CDS             3548..3979
                     /locus_tag="ACHAZB_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFM8615085.1"
                     /translation="MKRLKRSIFIVLALIDFVGTGCEAIDVQKAPLSEIKQAITKQDE
                     EQPLNTQVEIVNQNKPSFSEEDFSLENGTRQFFSDLDRLNRVGVASALLHKSRMPTEE
                     RDRLYIKPTGWKQKKMKNGDYLYNRCHLIGYQRATSFPISM"
     gene            4284..4466
                     /locus_tag="ACHAZB_002464"
     CDS             4284..4466
                     /locus_tag="ACHAZB_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8615086.1"
                     /translation="MQRTLSKSKNVGHEIRDGRLAVYLSRVGKTCLIDSDESGNATKR
                     FTEEIEQLAELNIRYF"
BASE COUNT         1688 a          726 c          972 g         1205 t
ORIGIN      
        1 tgaaaggcat tctccgactt ccaggttttt gaccattttc tctcaacgtg gaaaaaccgc
       61 cacttatgag agaaaatggt caaaaaccta agcgggaagt ctccgaataa cataccaagc
      121 gccgacagcc aacagataaa gaatacacta ctaaaaatag tagtagcata aaatttaaaa
      181 gggaaaaaat caacccctac cccagtcttg gggtctcaaa actaactccc ccctaacccc
      241 ctctttcagc ctcttttgaa agagggggag aatcaacccc acggccaacg ccccagtgtt
      301 cttggggctg tccagaacag aaacttgcga gagagtgggg gtaaaacaag tgtagaggaa
      361 acttgaagac ttgttcgaac agaaacttgc gagagagccg gagtaaaaca agtgtagagg
      421 aaacttgaag acttgttcga acagaaactt gcgagagagt tggagtaaaa caagtgtaga
      481 ggaaacttga agacttgttc gaacacaaac ttacaagaga ggggagtaaa gcagttgcag
      541 aggaatcagg aagatttgtt cgaacagaaa cttgcaagaa agcattatta aattgaaggt
      601 gatgaaatat gaagtattta ttgcatggct attttattga ttattctact cgcaatagta
      661 gtactttagc gattctatgt ttgcttcatg aattgagagt ggttactaga aaacagctat
      721 acaaactatt gaattttgac agatcaatta gtaaacaagg agtaacgaat gctttagtga
      781 gattatatga aaaacagtta atcgatcgaa aaaaatatgg aacagaaatg tattattatt
      841 tgacgaaaga gggacatcaa agcataggtg ggtattatac gttgccgaaa gttcctgaat
      901 ataatttgaa tcaccattta caaattaaca attatttgat taaaggaatg gcattggcaa
      961 aagaccaccc acatttaaaa cggattattt cagaaagacg taccgtctat gaaacgaagg
     1021 atttaaatcc taagacaaaa ggaagaaaat tttttgtgcc agattttatt tttcgttttc
     1081 gagacaaaga aaatcatgag atcaattggt cctttgaaat tgagctaacg ttgaaaacac
     1141 gtcgtcgcta ccgtgaagcg atttttccga aatatattcg tgagttgcga acacgtcctg
     1201 aatcacattt aatttatgtg acacctagtg gcgcaattca agaagaactt catgcgttta
     1261 agcgtatttt tttcaagcgc gaaactgaaa aagatgcgag tcaacaagaa ttgtttgagc
     1321 ggttgcatat tttttcagct ttagagtttg aagagggatt aaaagcatta ttagagaatg
     1381 atccagcagt caattggcgg acagtagaac gagaaaaaat ttatccgaat ggcgctgttc
     1441 aaagtcgttt gaatttattt gaagaatcaa ctgaaaatga ggaaaaacca aaagtgaagt
     1501 ttacgcctga agtattgtaa aagagggtgt tcgcttcgct ctttttttgt gccagtttgc
     1561 tggcaccgtt cagacgcaag cgtctgaacc ggctcgtttt taggaaggac taacttgttt
     1621 gatttatccg ttgtcaaaga agtggacgtt cgagagtccc taatcgtata gcagtcattc
     1681 ggcgagtata acctaaaaca taacctgcca attgccaagg gtaaaatgca cgctgcgcgc
     1741 ccttggcaat cgtcagaaca tgttttcgag ccggttatgc cgaacgaccg ctatacgaaa
     1801 agggcctcta aacagaagtc cactttgatc cttgacaact gaaacccaaa atcaaacaag
     1861 caggccttcg tctaaaaacg agccgaatga agaccttggg ggttgcaccc ccaaaccccc
     1921 gtgcccccga cacactaccg aaaaggtggg ggtgggtggc gaaaaaatca aataaaacat
     1981 tctattcaag aaaggaagaa acaaccatga caactaaaaa cgagcaactg aaagaacagg
     2041 caagaaaaga gttgcaagaa agaggtttga ttattgaggg gagctttgaa ggagattttg
     2101 aaacctatat tggttgttat gcaagaccga taaacaaacc tacagcctta gacccaatga
     2161 acgaacaaga agtaaaagaa caagaaaaac atgcagtgaa tggttttccg caagatttca
     2221 ctgaatggta tgagtgggaa attaacaacg ggaagcttga aaacttctta taaaagtcag
     2281 atgacgtgca aagagaatga ttctctttgt acggtaaaaa tataaagaaa gaaggaaaaa
     2341 gaaaatggca aaaaacacac cttatcaagt aggagacatt ttttatagta gttggggcta
     2401 cgaacaaacg aacgtgactt tttggcaaat tgtgaagtta acagaaaaaa cagcatggtt
     2461 tcagcctatt caaaagaaaa cggtcgagca ttattcgtcc atgagtggaa aagttgttcc
     2521 aatgaaagac aagtttatag agtatccttt agcaaaaact aaagacagta ttgtaaaaaa
     2581 acgaattgat ccagaaacct atcctgatat gttatgtggt tatcgtagtt gggacacgtt
     2641 tcatagatat aaaaatcaag ctgtttatga aagtagttat tattaagggg atagtttccc
     2701 ctttcaaaaa taataaatag aagaatggag agaaaaaatc atgatgaatt cagtgaattt
     2761 agtaggacgg ttaacaaaag aagtggattt aaaatacaca ccaaaaggca atgcgacagg
     2821 cacatttatt ttagctgtta atcgaaatta tacgaatgca aacggcgagc gtgaagcaga
     2881 ttatatccgc tgtgtcattt ggcggaaagc tgcggaaact ttagcaaaat atactggaaa
     2941 aggaagtttg attggcatta atggacgtct tcaaacgagg agctatcaga atcagcaagg
     3001 acagaccatg tatgttacgg aagttctagt gacagatttc tatttgttag agagtaaaga
     3061 aatcaatgag caacggtcaa aatcaatggc gaataatcaa aataattctt caattccagg
     3121 aaatgagatt caaatttcag atagttattt gcccttttaa gtgaaaaata attttgttct
     3181 atacctgatt tcttcaggta tagaacaaaa aataaataga gatgaggcga ttttttatga
     3241 aaatcattga cagttacgat caacgaacca tgcaatattt gggctcgttt gattatacag
     3301 atgaaaatat tattaattat gttgcaagtt tacctcctta tcaaagcgtt cgcttggtag
     3361 attttgcaag tgatgaattg gtattaacaa cgatgggcaa ctttttagat tatgtcggag
     3421 acaaaaaatg gttggaaaaa attttgtcta aattgattgc gaaacaaaca ggagaagaac
     3481 ccataaatgc gattcaattg ttcaatcgat atgaacaaga aaacaatgat taaagaggag
     3541 agaaaaaatg aaaagattaa aacgaagtat ttttattgtg ctagcattga ttgattttgt
     3601 tgggacaggt tgtgaagcaa ttgacgttca aaaagctccc ttatcggaaa taaaacaagc
     3661 aataacaaaa caagatgaag aacaaccgct aaatacacaa gtagaaatag tgaatcaaaa
     3721 taaaccgagt ttttctgaag aagatttttc gttagaaaat ggaaccaggc aatttttttc
     3781 agatttggat cgtttaaatc gggtaggtgt agcaagtgca cttttgcata aaagcaggat
     3841 gccaacagaa gaacgagacc gattatacat taagccaact ggctggaaac aaaagaaaat
     3901 gaagaatggc gattatcttt ataatcgttg tcatttgatt ggctatcaac gtgcgacaag
     3961 tttccccata agtatgtaat acgaaacggg aggattaatc ttaagattaa tcacaactaa
     4021 cctatttgac agttggaatg aaagctatca tgtctgctga atcaactggt ctgatactcc
     4081 tacatgcaat aaagtctact aggaagttat tgtatgaagc taggtaaagg cggcagaaca
     4141 atttctaagt gtggaatcat atgaagttgg atatgccggg acgctataaa ttactcaggg
     4201 tgagactgtt tttaattaaa ctgacgaact tgcgaatgta cgggtctata accgacttat
     4261 attcgaaaga tataagattg cgaatgcaaa gaacattatc gaaaagtaag aatgttggtc
     4321 atgaaattcg agatggaaga ctagcggtgt atctgtcaag agttggtaaa acatgtttga
     4381 ttgattcaga tgagagtggg aatgcgacaa aacgttttac agaagaaata gagcaactag
     4441 cagaactgaa tatacgatat ttttaaatac tttagcggag aataaaaaaa tcctctacag
     4501 aaaactacgg agggcaacat gtgctattgt ttcaacaata catttttaat ttttaccata
     4561 acaaaaaagt acttgaaaaa aaacagaaat a
//
LOCUS       DAYYCY010000175         4488 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000175 DAYYCY010000000
VERSION     DAYYCY010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4488
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.176"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            192..1079
                     /gene="xerD"
                     /locus_tag="ACHAZB_002465"
     CDS             192..1079
                     /gene="xerD"
                     /locus_tag="ACHAZB_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFM8615087.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            1098..1469
                     /locus_tag="ACHAZB_002466"
     CDS             1098..1469
                     /locus_tag="ACHAZB_002466"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8615088.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            1466..2236
                     /locus_tag="ACHAZB_002467"
     CDS             1466..2236
                     /locus_tag="ACHAZB_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HFM8615089.1"
                     /translation="MNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDIPIATITEQY
                     MNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEEDPREELVTQ
                     LLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKKIDLIRAMQK
                     ILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESPSKSEVVTTF
                     MALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            2220..2789
                     /gene="scpB"
                     /locus_tag="ACHAZB_002468"
     CDS             2220..2789
                     /gene="scpB"
                     /locus_tag="ACHAZB_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HFM8615090.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            2795..3511
                     /locus_tag="ACHAZB_002469"
     CDS             2795..3511
                     /locus_tag="ACHAZB_002469"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFM8615091.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     regulatory      3593..3718
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            3841..4452
                     /locus_tag="ACHAZB_002470"
     CDS             3841..4452
                     /locus_tag="ACHAZB_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFM8615092.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
BASE COUNT         1504 a          786 c          979 g         1219 t
ORIGIN      
        1 tttttaatac ctaaaatgaa aatggggctg aagaatcaat tctgattcat cagtcccata
       61 aattatagag ccgaaaaaag aaaccagtcc gtctgagtgt ctagacggct ttttctttgc
      121 tcagattatg gaaaaagact atcagaatga agcggtatct gatatgatag agaggcatga
      181 gggggattat tatgaaagag caaatcaacg attatctcca ctacttgaat attgaacggg
      241 gattgtccgc caatacacga caaagctatg aacgtgattt ggaacagtac ttgcattttc
      301 tacaggagca gaaaattgct tcatggcagg atgtcgatcg ctttttagtc atccgttttc
      361 tggagaagtt gcatgaagaa aaaaaagcga cagcaactgt tactcggatg atctcaagtc
      421 tcagaagatt ccatcagttt ttacggcaag agcgtatcac tgatcatgat ccgatgcaac
      481 atatcgatac gccgaagaaa gcacagaaat taccaagtac attgactctg acagaagtag
      541 aaaagctgat tgaagcacct gatacgacaa aaacgttagg gataagagat cgtgcaatct
      601 tggaagtcat gtatgctact gggatgcgcg tcagtgaatt gattggattg aaattgggag
      661 atcttcatct ttctcttgga ctggtacaga cagttggaaa aggagataaa gagcggatca
      721 ttcctttagg tgattatgcg atccaatggc tggaacgcta cttagacgaa gcacgtccat
      781 tacttgtaaa ggacgcatct gaaatgcatg tatttgtcaa taatcatggg aaaggtcttt
      841 ctcggcaagg aatctggaaa aatctgaagc agttagtacg agaggctggg atttacaaag
      901 aagtaacacc acatacgctg cggcatagct ttgccacaca tctgctagaa aatggagctg
      961 atttgcggac agtacaggaa ttgttaggtc acgcggatat ctctacgact cagatttata
     1021 cacatatcac gaagaaaaga atgacggatg tttataagca gcattttcca agggcttagt
     1081 cataggaagg tgagaagatg ttaacacatt accggaaaga aaaccgtaaa attgctatgg
     1141 gactattgag tttccataag aaactgacag gtaaaacttc tttgttgaag gagattgata
     1201 cgtacgaatc tgaagacgat tatgaacttt ttttctttac gccagaaggt tcaaccaaca
     1261 ttcaagggat tattggtatt tactgggcaa cgaacgatca gctgatcatc catgacattt
     1321 ctttgaatcc gtcttatcgc ggtgagaata tgggctttca aatgttagat gaattacagg
     1381 aaaagtaccc ggatgtctcc atccgtgcaa ctgaaatcac agagccttac attggaaaat
     1441 ggctagcaaa gaaaaagaag aaagagtgaa tgagttgacg gagatcaata ttaaattaga
     1501 tgtattcgag ggtcctctcg atctgctgct tcatctgatt cagcaaatgg aaatcgatat
     1561 atatgatata ccgatcgcta cgatcacgga gcaatatatg aattatatcc atacaatgaa
     1621 aacactcgag ctagctgttg caggagaata cttagtgatg gctgctacat tgatggcaat
     1681 caaaagcaag acattgcttc ctgtccaaga attcggcaca gacgatgaag aattatggga
     1741 agaagatcct cgagaagaat tggtcactca gcttttagaa taccgtaaat ataaatatgc
     1801 agctgaaaga ctgacagaaa aagcagagga acgaagtctt tattacacaa aagaacctat
     1861 gaacatcgat gggttagcgg atgctgaaaa gccgttgaag cgaggacaag tcaaaaaaat
     1921 cgatttgatc cgtgccatgc agaaaatctt agaacgtaag aaattttctc aaaaagtgga
     1981 aaaaacgatc acgcccgatg acacaacgat cgaagaacga atggtattta tcgaagagcg
     2041 tataaaaaga aatgcgcatg gctgtacttt cgattctttt tttgagtcac cttctaaaag
     2101 tgaagttgtc actactttta tggctttgct agaattgatg aaaaaagggc agatcattgc
     2161 gcaacaaaaa gaaaactatg aatcaattat gctttatcca gcagtaggag ataccgaaca
     2221 tgacgaaatt aagtgaactt gaagcccttc tttttattgt tggcgaggaa gggatcagta
     2281 atgaagaatt aagcggactg ttaaatctca gcgaagcaga agtcacaagt ttagtgaaag
     2341 aattaaaaga atcttacact caaaactcgc actcagcttt gcatattttt gaaacggaaa
     2401 gccattatgt attaacgact aaaaaagagc tggctccctt acttaagcga tatgcgcaag
     2461 ggacatcaaa cacattatct caagccgccg tcgaaacctt agcaatcatc gcttataaac
     2521 agccaatcac aagagctgaa atcgagcaga ttcgaggagt tcaggtttca ggagcggtac
     2581 agcgtctagc tgcccataaa ctgattgaag aaaaaggtag agtagaagga ccaggtcggc
     2641 cgatattata tggaacaact ggctacttct tagattattt tggtttgaaa tcattagaag
     2701 aactgcctga tacacaagaa atggaaattt cacttgaaga agacgtgtca ccagatctat
     2761 tctttgatgg aatgaataaa ggagaataat tgtaatggag agattgcaga aagtaattgc
     2821 ccacgctggc atcacttctc gtcgaaaagc agaagaattc atccttgcag gacgtgtaaa
     2881 agtaaacgga caagttgtaa gggaattagg gataaaagtt gggaaacatg acactgtgga
     2941 agtggatgaa gtcccaattt atcaagaaga atacggttac tatttattgt acaaacctaa
     3001 aggagttatt tctgctgttg cagatgacaa aggaagaaaa gttgttacgg atcttttgcc
     3061 gatggtaaaa gaacgtatct atcctgtagg tcgactggat tacgatactt cggggatctt
     3121 gctactgaca aatgatggag attttgctca gcgcctgaca catccaaaac atgaagtgga
     3181 taaagtttac gttgcaaagg taaaagggat cgctacaaaa tcgatgcttg cacctttgac
     3241 aaaaggaata aagatcgaag gacgaaaaac atctccagct aaattccaaa ttttatcagt
     3301 ggatcctaag acaaaccaca gtatcgtgga attgacgatc cacgaaggac gtaatcatca
     3361 agtgaaaaat atgcttcaag cagttggtct tcctgtgcaa aaactaaaac gtgaaaaata
     3421 cggagatctg actttacaag gcctacgtcc tggggattac cgtgaactga acaaaaaaga
     3481 agtcagcagt ttgctgaacc actcaaaatg aacacttact ttttataatt gttagatgtt
     3541 gcgttttttg aatagatatg tataatgaat atgtaaacaa aaattatata aggttcgtct
     3601 tcaggggcag ggtgaaattc ccgaccggtg gttatagtcc acgacctatt ttgcaaaaaa
     3661 atagctgaat cggtgaaatt ccgataccga cagtatagtc tggataaaga agatagagct
     3721 tatttgacga cgcttgatca aataactcta actctatttt ccctggaaaa tagagttttt
     3781 tcatttggag agaaacgtca aaaagttcgc tgaagaggag tcctaacagg aggatttcaa
     3841 atgaagaaca cccgtgtaca gaaaatggtt gcagtagcga tgtttgctgc gatcgcatta
     3901 gttttacagt attttgcttt tccgatcatg ccggctttta gttttttgaa gatcgatttt
     3961 agtgatatct cggtattgat cagcatgttc ttattcggtc cagcggccgg agttgcaaca
     4021 gcgttcttgc gttcagtatt acatctgctg actacaggtt tttctccgga taatttggta
     4081 ggagacactg ctagtttctt atcaacaacg atctttacat tcccgattta ttatttcttt
     4141 agtgtaaaga aacagcataa aggaagaaac aaatttttag gtgtcattac aggaaccatc
     4201 gctctaacta tttttatgag tatcgcaaat tatttcgtta ttacgcctct ttacttaatg
     4261 tttttagggc tgaacgcaac acagatgctg ggcatgtctt tagcaaacta tgtactgatt
     4321 gggatcgttc ctttcaactt gatcaaagga ttcatcgtga gtgctgtctt tttagtgctg
     4381 catgcgcaac ttcttccatg gttaagtcga aaacaacatc agttcgaaca acgtcataca
     4441 gcagtcaaat aaatatcaaa aagatcaaga aatttcttgg tctttttt
//
LOCUS       DAYYCY010000176         4475 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000176 DAYYCY010000000
VERSION     DAYYCY010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4475
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.177"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            326..1054
                     /locus_tag="ACHAZB_002471"
     CDS             326..1054
                     /locus_tag="ACHAZB_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFM8615093.1"
                     /translation="MKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEAG
                     DEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYAA
                     ADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAVS
                     LVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSVP
                     GKSVKLAQQYSTKLVWTLEDTPNN"
     gene            1199..3226
                     /locus_tag="ACHAZB_002472"
     CDS             1199..3226
                     /locus_tag="ACHAZB_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HFM8615094.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGHSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            3334..4431
                     /locus_tag="ACHAZB_002473"
     CDS             3334..4431
                     /locus_tag="ACHAZB_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFM8615095.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
BASE COUNT         1574 a          717 c          923 g         1261 t
ORIGIN      
        1 aaaaaatata atatacattt tttatttaat tataattaat attgattgaa gtctaggcag
       61 tcaagtcaca ttttgataga taggaggggg acttacgaat tattttaaag gctatgacag
      121 ctgtatttct agattgtgtt tgcatcaagt tgcaataaaa actagaaatc aaatcgggct
      181 gataatcatt tataactaat cgatacatag gaccaaatat aggtctatgt tcttttattt
      241 cataagtatc gtaaaacaga attttgctat ggattaatcc aatcgactga tatgtttagt
      301 aaataaagaa agaggaatgt caatgatgaa aaaagaaatc gtattaacag cagcaatgat
      361 gttattcagc atggtagcaa gtacgacttt tgtttctgca actgaagtat acccaaagga
      421 atataacaca gaaggaacga ttacatttga agcaggagat gagggtgtaa ctcctcctgt
      481 tgatccagag aatcctgatc ccaataaacc tgtcgatcca tcagatcctc caagccctgg
      541 aacaggaggg gcattatcga ttgattatgg atcaaaattc aaatttggga ctcaaaaaat
      601 ttctactgcg gataaaacgt attatgcagc tgctgatgtg atgaacgatg ggtctagaaa
      661 accgacatat gtccaagtga cagataggag aagtacctta tctgggtgga aattaagtgt
      721 ctctcaacca gaacagttta aaactgccag tggggatgaa ctagttggag cacagctgaa
      781 atttacgaaa gggcaggctg tttctttggt tgatccaaca tatacgccgc aaacggtgaa
      841 ttctgaatta acgttgacac cgggaggaaa caatacgtta gcaattaatg ctaaatcagg
      901 aacaggtgtg gggacttggg tctatcgatt tggtgcaaat gaaaatgaaa atcaagatgc
      961 ggtacagctt tcggtacccg gtaaatctgt caagttagcg caacaatatt ccactaaatt
     1021 agtttggaca ttagaagata cacctaacaa ctagtttact gaaaagaact gttaaaaata
     1081 aatacgtagt catattgtct tgcgaggtaa tcgagcaaaa attgtcaaag gctaggctaa
     1141 atttgtttgg atacgaatag ttcatctagg aatttaacga tcaggaggaa aagatatatt
     1201 gaaaaagagg aggatcgtgc tcattttaag cagtctctta gtgactaata tctttccgat
     1261 catagggtac tctttggaat tgaatcagtt ggagcatgga aatagaagag acgaagaaag
     1321 caaactcact tcgacattcg acagtttaat agaatccaac cagacacaag aaagcagtga
     1381 ggatttaagt atttcgagta gtggagagac cactgacaac gtagaagctg agggttcgga
     1441 caaacaagga ctagaagatg caccaatttc tgcggttgag ctgcctgacg atcccaatat
     1501 tatttctatc gataaaattt ttcaaacacc tatcggtact agcacgtcca tattggaaaa
     1561 tgggaagtta ctgcagttga acccagcaga aaggtctcaa agaggagcta tttggtcaaa
     1621 taagaagatt agtttactct cggattttac atttaaatct tatttatact taggaaatca
     1681 acgagataga gcgggagatg gcatgacctt tacattgact ggcgatccta gaatggctag
     1741 taatcccagt caagttattg gatcgccagg tatgggaatt ggctcatatt ctactcgttc
     1801 tggccaacct tacattgcga atgcgctctc gattgagttt gatacgtata aaaatagtgg
     1861 ttcttctgat cgaatggatc gagagataac tgctgataat agagggcatg gacatgtcgc
     1921 ttttgtcacg cctaaaccaa ataataataa ttattctgga gagcatactg gcgtgacggt
     1981 agctcctaca tttctttcta atggcacatg gcgcttgtta ataattaaat gggacgcgga
     2041 gcaaagaagt cttacttatg atttggaagg tgtcggtcac tcttctcgga ttatacctga
     2101 tctcattagg cagtttggtt ctacagaagt aaattggggg tttacttctt caacaggtac
     2161 tttttttcaa gaaaatgctg tcgctgtcac gcgacttcca tcgaatgtca catcaacagc
     2221 tgatgtaaaa gtcaacgata gtgattatgg ggccatcgct gaagctggag gaaacgataa
     2281 gataactatc agaaatacat tgaaggtcga gggggatttt cttgaatata agaaaatgca
     2341 gactagtatc gacctcccac aagaattaac tattcccgag catatcttga tcaatggaaa
     2401 tcaaattgat gccaaagata tcttgataac cggaaatcga gtcacgctaa atctgacaga
     2461 ttattttatt gctggagata cgttaataat cgaattggag acaactttgc aggaagtaat
     2521 tcctgaaaaa gtactcacta tgcattttga ataccttgaa aacgggaatt tgattgagca
     2581 atcgaatgaa ataaacctaa agatcgcaaa gctgaaggag atagatatcc atgtttatta
     2641 taaagataaa gacagtcaga ttgatattgc tgaaatgaaa acaatcactg ggaaaatagg
     2701 agagaagtat aaagaaactc ctattgatat tgaagggtat gtttttgact cggattcagc
     2761 gaatacagaa ggcatcttct ccgaaaatac agaggatatc tatttttatt acagaaaagg
     2821 caaactttat ctctcaactg ctcctcaata tgtcaatttt ggaaaacata aaatatccag
     2881 tagaccatta agtgaattcg gacagttttc aggtgggttg aaaattatag atgaacgggc
     2941 ttttggaagt tggaaattac aattgaaaca agagaaagtt cttacaaatg gagattttga
     3001 gcttccagaa gctctttcct ttgttacctc tgaaaataca acggttatag ggacatccgc
     3061 tgttacgatt ttcgaaagtg atcaaaaggg tgaaagcgat ttgtcctctc ttttagattc
     3121 tacagctcat cgtggaatca gaatagatat tcctgtagaa aaccaaagaa tcggaacctt
     3181 tgaggggaaa ctatcttgga tacttgcaga tgtcccagga aattaacagg aagagaagaa
     3241 tataacaaaa ttattgagtt tcaagaaggg cacaggtacc ataggctctt gtgtcccaca
     3301 gagacaagat tttgttatag tcttctctta catatgatta aaggagcgat tttcttgaga
     3361 ccaaaacaac aattatttat aacactccta gttattttag gggcactttt taacaatata
     3421 tcagtgtcag ctagtgcatt taatttttca gtgacgccga taacttcaga gaatcaaatc
     3481 gacaagagaa aaacttactt cgatcttcaa cttgatccgg atcaggaagt agaagtaaaa
     3541 gcggaattga gaaatgatac agaaaaagaa gtgaaaatcg atatttctgt gaatagcgca
     3601 accaccaatt caaatgtgat ggttgaatac ggaaaaaacg agattgaaaa agacatgagc
     3661 ctgatctttg atctggtaga ttatgtgagc tatccacaga tagtgacgct aaaacctaaa
     3721 agtgtccaaa ccgttgcttt tcatgtcagg atgccgaatg agcgatttga tggtgtattg
     3781 gctggtggga tcacattcca agaaatccag acggaaaagg accaaacaga aactaaggat
     3841 caaagcttat cgattgagaa tgaatacacc tatattattg ctttgttgat gcaacagaca
     3901 ctgaacgaag ttgctccgaa ccttactctt catgaagtta aaccagatca aatcaatgca
     3961 agaaatgtca tcttagcaaa cgttcaaaat gatcaaaaaa cctatatcaa tcaagtagtt
     4021 attgaaacaa aaattacaaa aaaaggacat tctgaagttt tatatcaaga ggaaaaagaa
     4081 ggattgcaga ttgcgccgaa tacaaatttt tcttttccaa ctgctttaaa tggtcaaccg
     4141 ctaaccccag gtgaatatca ccttacaatg actgttttag gaaatgaaaa tgaaaatgga
     4201 aaattttcga gaaaaaaagg aaatactacg atcaactata ccaatcaatg ggtatttgaa
     4261 aaagatttta caatagacgg aaaagtagcc aaggaattga acacgacgga tgttacgatt
     4321 aaaaaagaca ataattggat ttatatctta gtagcttcac ttttcttatt gtttatttta
     4381 ttatgcatac ttttgtttgt tttgagaaaa aaagaagaga aagaaaagta aggttaagat
     4441 aacaaatagt aaatttttat tattgtagga tggtt
//
LOCUS       DAYYCY010000177         4436 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000177 DAYYCY010000000
VERSION     DAYYCY010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4436
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.178"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..779)
                     /locus_tag="ACHAZB_002474"
     CDS             complement(<1..779)
                     /locus_tag="ACHAZB_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8615096.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDL"
     gene            complement(781..2220)
                     /locus_tag="ACHAZB_002475"
     CDS             complement(781..2220)
                     /locus_tag="ACHAZB_002475"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004564 - beta-fructofuranosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFM8615097.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2405..4351
                     /locus_tag="ACHAZB_002476"
     CDS             2405..4351
                     /locus_tag="ACHAZB_002476"
                     /EC_number="2.7.1.211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-specific PTS transporter subunit IIBC"
                     /protein_id="HFM8615098.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKINGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         1406 a          763 c          737 g         1530 t
ORIGIN      
        1 tttaaatctt ctgggatatt aagtttaagt tgatgagcaa ttttcatagt gagaatggct
       61 gttaaatcat cagaaacaaa aataccatct ggtttgtgat ttataatagt tgactttatt
      121 tccatttcac gacggatagt agacagatta ttaggaattt tgaaaatttt tccatttgga
      181 ttttgaaaag aaaaacctag tgcacgtaat tctgtgggag aatccgtgtt atcatttcct
      241 gtaatcataa tggtattttg acaaccattt ttttgtaaag tcttagctgc aagttttccc
      301 ccttcaaagt tatctgaaga aacaatggaa acattaggag ctaaatttct atcaaaagca
      361 acaataggtg ctctcacacg ctcataatca gcaattccaa gattatggct tgatgagatt
      421 atgccatcaa cttggttggc ttcaagcatt tcaatgtatt ctctctcttt agcaggatca
      481 ttttgactat tgcaaataat cgttttatat ccatgtttaa ataactctat ttcgagatac
      541 tcaatgagtt cagagtaaaa gatattacta atattaggaa aaataagacc aataagctga
      601 ggtgactttc cttgaagtcc acgtgctaaa ttatttggtt tataaccaag agttttcata
      661 gcttcatgaa ctttttgttt tgttttttct gataaatatc ccttattatt aatgacgcgt
      721 gaaacggttg ttgggctaac tccagctaat tcagcgacat cagttaattt tgctaccatt
      781 ttaatatttt aattcaaagt attttccagt aggttcgcct gatttgattt gaataccatt
      841 ttggtgattt tcagggaata cacgactagt aaacactttt tctcctttat taataaaaat
      901 ctcaatgatt gagttatcta caaaaatgtt agcagttgtt tctttagtat caattgagca
      961 ttcacgaatc gtaccaaagt cagtggcata ttgaatgcct gctttgctgc gatctaaaat
     1021 aatttttcct tctttggtat ctacaatcaa gctaagtcca tttcctttac tatcagagaa
     1081 aagaagtaac tccgatttct gatttggagg aaaagttagt tcaagctcat aagtattatt
     1141 tgtttctgct tttgctgtaa attgttcaga actagcacgt aatgatgtaa tagcttctac
     1201 tggatattgg taaagtttac catcttttat tgaaagttct tttacaagac tcattgctcc
     1261 ctgataatca tacttatcag ttggataatc gatatcaggt agtccaatcc aactgacaat
     1321 cagagcacga ccatcaggag tattgaatcc ttgagtcgca tacgcttcaa atccataatc
     1381 aagattttga atttctgaag cgcccacaag ttttactttt tctatgtcaa gcgattgaca
     1441 gatcttaaaa gtattcggat agatattccc ataatttagc tcagatttat ccaacccttg
     1501 aggacaataa atgagaacag gttttttgtc tacaaatact agatttggac actcaatcat
     1561 gtattctgaa ccagtgccgc caaaatcaag gttgccaact tcctcccatg tttccacgtt
     1621 attgtcatcc gctttgtaaa gtttgataaa gcctgatttt tctaaatttt gtgctccaac
     1681 aatcgcatac aattgtcctt cataattaaa catttgtgga tcacgaaaat gatcagttac
     1741 atcagcaggt ttatgaataa gaacattctc acctttcgta atgttgtagt ctttatccat
     1801 ccatgctccg atttgaagtg tatcacgttc ccaattttca tttcgaacgt ttcctgtata
     1861 gagtaagaat agattcccct ctatttcata tgcagaaccg gaataagcgc cgtggctatc
     1921 tcgttttgtg tctggaagaa gttctgctcc tgttacatga aaatgaacta aatcatctga
     1981 ttcattgtgt acccattctt tcaacccatg agcagctcca aatggccaat tttgataaaa
     2041 aagcgtatat tttccgttaa aaaaagaaaa accattagga tcgtttaaca atcctgtttt
     2101 aggttcgata tgatagctag cacgccaagg tgatcgtttt acattatcct taattttatc
     2161 catctcatct ttagtccaat cagaataagc acgatatcgt atttctcgag gtaagttcat
     2221 attttactcc ttcaatttat ctttttacaa ctagtatggt aaacgttttc cgttgaaaaa
     2281 tcaatcattt catttaaaaa aataaactat atgacaaacg tttgacatat ttttaagata
     2341 tgctaaaata aatttgcatt tcagtgaacc gttttcacgt aaaaataaaa aaggagtgat
     2401 tcgaatgaat tacagtaaag tagcttccga cgtaatacaa gcggtcggaa aagataacct
     2461 tattgcagct gcacactgcg ctacacgtct tcgtctcgta ctaaaagatg acacgaaagt
     2521 gaatcaaaaa gctttagacg aaaatccaga tgtgaaaagc acatttaaaa ttaatggaca
     2581 atatcaagtg attatcggtg caggtgatgt taatttcgtt tatgacgaac ttatcaaaaa
     2641 aactggactt tcggaaattt caacagatga tttaaaacaa attgctagta aagataaaaa
     2701 attcaacccc attatggcat taattaaatt attatcagat atttttgttc caattatacc
     2761 tgcccttgtt gcaggtggtt tattaatggc attacaaaac tttttaacag ctgctggact
     2821 ttttggacca aaatcaattg aagaaatgta tcctgcgatt gaaggtattt cctcaatgat
     2881 ccaactgatg tccgctgcac cattcatgtt cttaccaatt ttagtaggta tttcagctgc
     2941 taaacgtttt ggagcaaacc aatttcttgg tgctgcaatt ggtatgatca tgacaacacc
     3001 agatcttggt ggctcaactg aatattggaa tatttttggt tatcatgttt cacaaacaaa
     3061 ctatgcttat caagttattc cagttttagc agctgtttgg atattatcta tattagaaaa
     3121 attctttcat aaaaaattgc catcatctat tgactttaca ttcacgccat tattatcagt
     3181 aatgattact ggatttttga ctttcacagt tattggccct gtaatgttat tgttgtctaa
     3241 tggaattaca gacattattg tttggctata taacactact ggatttattg gaatgggtat
     3301 ttttggagga acatattcac ttatagtcat gactggttta catcaatcat ttccagctat
     3361 tgaaactcaa ttactttcag cgtggacaaa tggtatcgga catggagact ttatctttgt
     3421 agttgcatct atggcaaacg ttgctcaagg tgctgcaaca tttgccattt ggttcttaac
     3481 taaaaattct aagacaaaaa gtcttgcttc ctctgcgggt gtctcagcac ttcttggaat
     3541 tacagagcct gccttgttcg gagttaactt aaaataccgc tttccattct tttgtgcttt
     3601 aattggttca ggaattgcta cagcagtagc aggattactt aaagtagttg ctgtttcact
     3661 tggttcagca ggtttcttgg gatttctttc aattaatgca acatctattc cattttacgt
     3721 attgtgtgaa ttgattagct ttgtagttgc ttttgcaatt acttacttct acggtaaaac
     3781 aaaagctgct ggtatcttta aagcagaaga aattgctgaa caaacaccag ttaattcttc
     3841 aaaagtagca acaacagatc caatatctac tgatgttgaa tcaacacaaa caaaagtttc
     3901 atcagaaaca attgttagtc ccatttctgg taaagtgatt agtctttctt cagtaaatga
     3961 tccagtcttt tcatcagaag ctatgggaaa agggattgcc attaaaccaa atggaaacac
     4021 ggtccattct ccagtagatg gtactattca actcacgttt gaaactggac atgcttatgg
     4081 tattaaatca aataatggtg ctgaagttct aattcatatt ggtattgata ctgtatcaat
     4141 gggaggaaaa ggatttacac aaaaagttac tactaatcaa aaagttagaa aaggcgatat
     4201 tcttggagca tttgaccgtt caatgattga gaaagaaggt cttgatgata caacaatgat
     4261 tattgtgacc aatacttcgg attattcaga agtaaatata gtagctgaaa atacagtcac
     4321 tgagggtact gatttacttg aaatcaaata aatatatttc cctgagtaaa gatggaattt
     4381 tctttccaaa atcaaataat atagattaat caaatatttg agaataacat tttatt
//
LOCUS       DAYYCY010000178         4425 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000178 DAYYCY010000000
VERSION     DAYYCY010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.179"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            690..1658
                     /locus_tag="ACHAZB_002477"
     CDS             690..1658
                     /locus_tag="ACHAZB_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM8615099.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            1669..2484
                     /locus_tag="ACHAZB_002478"
     CDS             1669..2484
                     /locus_tag="ACHAZB_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HFM8615100.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKEIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            2552..3262
                     /locus_tag="ACHAZB_002479"
     CDS             2552..3262
                     /locus_tag="ACHAZB_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8615101.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            3298..>4425
                     /locus_tag="ACHAZB_002480"
     CDS             3298..>4425
                     /locus_tag="ACHAZB_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFM8615102.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
BASE COUNT         1490 a          661 c          921 g         1353 t
ORIGIN      
        1 atacaaataa ttcaccaaaa aggtgagtaa tgccattttt gaaaattgag cttgttttat
       61 gtctacatct gtatccgtat ataatccaaa ggattataaa ctcagaagag tctttatttt
      121 ttctgaatat aactactgta tcaaaaacaa tcgattatga gacaaaacaa aatgacctct
      181 tttatttaag aaaggaggtc attttttgta tcataaattg tattaaaatc aatgtatcac
      241 tttttaatca gttttaagac cataaattgc gtactaaaag aaattctgca attcaaaata
      301 aagcatttta agccattttc aaatttaagt gcacccatct acccgtcaaa tcactttatt
      361 ttccactcca gtagtttcct aattcaactg tgtgcaaaaa cgatttcttc taaacaaatc
      421 aagacaaact tataaacctt catcccagca ccataattat ttaaatcata ggcatttttc
      481 gaaattagta tatgtactca agtttcactg cagattattt atatttttta gagaaataaa
      541 tttttcctat tgacaaacac aaagaaagcg gatacaatgc aattatgtca catgacgtta
      601 tatgacgtta tgtgatatct ttacatttaa tcagttactt ttattttgga taaaattaac
      661 atttttaaaa ttttaaggag gaaatgttga tggatgtagc aaaaacggtt aacgcaattg
      721 tagaaaaaaa tgatggagag atttctggaa tttatttggt ggcttgtggc ggttcgctgg
      781 tagatatgta tgtctcagat tattttctta aagccgaagc atcgaagtgt attacaggct
      841 tatatacagc gaacgaattt aaccatgtaa ccccaaagca attagatgat aaatctgttg
      901 tcattctatg ttctcatgga ggaaatacag cagaaacggt aaaagcagga gaagtcgctc
      961 aagctagagg agctgtgact gttggtctga ctcataataa aactgcggat ttattaaagg
     1021 tttctgatca tagtttcctt tacgaatggg gagatgatgc aaaaatagtc aataatccta
     1081 tggccatcat tcttgattta actgtatctt tggtcaatgt taccgaaaca tttgaacatt
     1141 acggtaaatt cagagaaggc atgaaggtta tcgataccgt aattgaaaat ggcattaagc
     1201 aggtgcagga tcgtattaaa gtgtttgctg ataagtacaa agatgaaaca atgttttata
     1261 tcttaggtag tggaccatca tatggacatg cttatggttt ttcaatttgc tctttgatgg
     1321 aaatgcaatg gcttgattca acttctgttc attctggaga attcttccat ggaccttttg
     1381 aagtaacaga caaagatacg aattttattc tcttgatgag cattggacgc actcgtgctt
     1441 tagatgaacg ggcaaaacga ttccttgata aatatgcaga aagagtagaa atcgttgatg
     1501 caaaggaact aggattggat atgatatcag atgaagtttc tgaattcttc aatcccattt
     1561 tattctacag tattctttca gaatatcgtt cagctttagc agatgtacgc aatcatgatt
     1621 tagatgttcg tcgctatatg gggaaagtgg agtactagga ggattaaaat gaaggtatta
     1681 ggcttaggtg ataacgtcgt cgataaatat gaaaatctgg gaattatgta tcctggagga
     1741 aattcattga attttgcagt ttttgcaaaa aaactgggac atgaaagtag ctttatggga
     1801 gtttttggct cagatgagga ggcagctcat gttttatcag caataaaaga aataggactg
     1861 gacaacagtc actctcgctt tgaaaaaggc gagaatggct gtgccagagt gaagattgat
     1921 gagggcgacc ggattttcct tggcagcaat tcgggaggtg ttactcgtga atacccgatt
     1981 cagcttactg aagaagacag agaatattta aaccagtttg aattgattca tatgggatta
     2041 tatagccatg tcaatcatct attagaagag ttacaaaatg tttctggaaa gatttcttat
     2101 gatttttctg acgactttac ggaagaagag gttcagcggg caattgataa agttgatttt
     2161 ggcttctttt ctatagaaga cttttccgat gaagaagtaa agcagttttt gatgaagcat
     2221 ttttgtgagc gaaatgatgt attgattgca acccgaggag aaaaaacggc tattggttat
     2281 gatggagcaa agttttatga agtgaacccc gtgctagaag aaccagttga tacaatggca
     2341 gcgggagatt ctttcttaac agctttccta ctttattttc tcgaagggga cgatatagtc
     2401 accgcgatga aaaaggggaa tgaatttgct gggaagtctt gtctggttga cggttcgttc
     2461 ggctttggta ttcattacga ttgagcttgt tttaaattcc tgcatttgat aacctaaaga
     2521 aagaaatccc ggtaaagtgg aggttaaaaa aatgctggat acaaatgcac aattaccatt
     2581 gtacttacaa ttaaaagaaa tattaaaaaa taaaatcaat acaggggagt atcccgagaa
     2641 tacgaaaatt cccgctgaat cagagctgat agaaagttat gaggtcagcc gaatcacagt
     2701 gagaaaagca gtagaagaac tggtagaaga agggtaccta cttaaaaaac aagggaaagg
     2761 aacttttgtc aaccggcata aagtttttcg taaaattgaa tatattatgg gtttcagtga
     2821 gagttgtaaa tctaatggtt ttacaccgac tagcattgtt ttggaacgca agatcattcc
     2881 agcaactgaa gggatggcaa aaaaactcga tatcgctcaa ggtgatgatg ttttatatat
     2941 ccagagaaag cgaatggcgg ataacatccc cattatgtta gaaaacaatt atttttctta
     3001 tgaaagatat agtttcttaa tggaggaaga gctgactgat tctttgtatg aagtacttaa
     3061 agtccaaggt accttagcca tcaatcctgg ggaaactgca cttgaaattg tcacagctga
     3121 tgaccaaatt gccaaaataa tggaagtagc tgttgggacg ccctttttct ttatggatac
     3181 tttaatcaac gatcaaaaca acataccaat tcatgtaggt aatcaatatt acctgggaga
     3241 agtatatcgt ttttacttat gatttcctgt ttggatttca aggagtagat tttatttatg
     3301 aagcaaatat tgcggtcaaa aatgattttt gatggagagc agccaccttt caaaggttat
     3361 attgtaatca atgaagggat cattgaaaag gtggaaaaca actggaatta cagtgatcta
     3421 ttaaatgaaa acacggaatt aattgcttat gatgatcaat tcgttatgcc agcaattcat
     3481 gataatcatg tatttttcag tggttatttg gctatgaatg ctggcataga tttaagcgat
     3541 acatcttcag ctaacgaggc tgtagagcgt attaaagctg taatgaacca taaaaaagca
     3601 gatgaaccaa tttatgctca tggctgggat gctgccctgt ggaaaactga cccatcagaa
     3661 gagctattga atgaaataaa ccactttggt ccaatcacag cgatcgacaa aaatcgcagc
     3721 cattgttgga tgaatgctat tgctaagcag acttatggtt ttacagaaaa agaaaccagt
     3781 gctgaaagtc gagtattatt aattaaggaa atgctagcaa acaaaaagct tttagcagag
     3841 gtttacgctg aatttgaagc attactgctt tcaaaaggtg ttatatcaat gaaagaaatc
     3901 gtttttgatg acgcagaatt tctttctaat atacctcaga ggaaaatact tagtaaccta
     3961 tatgtgcagg cagtccaaga acctctacag catgaacttc tatatgagta taaggaaaaa
     4021 gttttccctg aaaaggtccg ctttggtgga gtgaagatca tggtagatgg tgttgttgcg
     4081 gatagaagtg gagatattta cggtgtttat cagtcaggag tagcttcacc cgagattgac
     4141 tatgaggcaa ttaaagcaga agttgctgca ttcaatgaag tagagattcc ttgctgtttg
     4201 acaacggaag gagataaagc gggttataaa gctgcgtcaa ttcttgtcga atttggaaag
     4261 cggttacctg aaggcgtttt taattcgatt agtgatttgg aaatggttac tcaaaagaca
     4321 gcagaaaaaa tgaacgaagg acaagtagta gcagaggtat atccacaaat tctcggcttg
     4381 aatcaaacgt tagaagaggc ttacatgtca actgttataa aaaat
//
LOCUS       DAYYCY010000179         4400 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000179 DAYYCY010000000
VERSION     DAYYCY010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.180"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..218)
                     /locus_tag="ACHAZB_002481"
     CDS             complement(<1..218)
                     /locus_tag="ACHAZB_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFM8615103.1"
                     /translation="MYRKNSSGWSKHIDFILLDLFCVQLAFYISYVMRQGDWNPYVIP
                     IYRHMAIFIELENLTIIFLFEAYKNVLK"
     gene            complement(262..1026)
                     /locus_tag="ACHAZB_002482"
     CDS             complement(262..1026)
                     /locus_tag="ACHAZB_002482"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HFM8615104.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKIPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKEFG
                     KEKVVQMKQVAKDLINGDEINYPIYEEVKKKKFGLF"
     gene            complement(1054..1752)
                     /locus_tag="ACHAZB_002483"
     CDS             complement(1054..1752)
                     /locus_tag="ACHAZB_002483"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HFM8615105.1"
                     /translation="MARTQKQKAAQTHAVSLVTLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVDNLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(1764..2543)
                     /locus_tag="ACHAZB_002484"
     CDS             complement(1764..2543)
                     /locus_tag="ACHAZB_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HFM8615106.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGPAQTQPKATNDKTAPSRRSRSRV"
     gene            complement(2559..3503)
                     /locus_tag="ACHAZB_002485"
     CDS             complement(2559..3503)
                     /locus_tag="ACHAZB_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFM8615107.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(3549..4328)
                     /locus_tag="ACHAZB_002486"
     CDS             complement(3549..4328)
                     /locus_tag="ACHAZB_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8615108.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADIQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTVLSQIEQIRNEKISAAYATKAQTVAEQMGYSNISY"
BASE COUNT         1120 a          928 c          805 g         1547 t
ORIGIN      
        1 ctttttaaaa catttttata tgcctcaaat aaaaatataa tcgtaagatt ctcaagttct
       61 ataaaaattg ccatgtggcg ataaatagga atcacatacg gattccagtc accctgtcgc
      121 attacatatg atatataaaa agcaagctgg acacaaaata agtctaataa aataaaatca
      181 atatgtttag accaaccact tgagtttttt ctatacatct ctcactctct cctcatttcc
      241 tttttatcta cgaataaaga actaaaataa accaaacttc ttctttttta cctcttcata
      301 gattggataa ttgatctcat ccccgttgat cagatccttt gcgacttgtt tcatctgtac
      361 tactttttcc ttaccaaact ccttggcaat ctgctcatag cactctttta agtaaaaggt
      421 gcgcttagag accccgtgtg catcagaagc agccatctgc acgagattat gtttgaccat
      481 tgttttggct gttttctgga tatctttgcc aaatacacca acataacttg gcgcagtaag
      541 ttgggccaaa catcccatat ctaggaacgg aagcaaatga tttgggtctt ctcgaaaatg
      601 cgcgttgcgt tctggatgga cgatcactgg tatctttcct agttctaaca actcaaaaaa
      661 aagccgttct gcatataacg gcacttccag tgtcggaaat tcgatcagaa gatatgtatc
      721 atctaaatct gtaaataaaa tttcatctcg gtggatgtct tcgatgagcg ttccggtaat
      781 acgtacttct tgtccttcca ataacgtcaa aggcaactgt cttttctcta attcggcttg
      841 taacgaagcg actagagaaa tgacttgtga tttttcattg ctgtattttc cattattgtg
      901 gtgtggtgtg cataaaatat gcgtaatgcc ttgctgaatc gctttttcag ccattgcaat
      961 acttgcttct aagttttctg ccccgtcatc gattcctggt aggatatggc aatgtagatc
     1021 gatcatttct cttgtctcct ctcttcttat tccttaattt ccgtagtaat aaccagcatc
     1081 tttgctgtgt tctgctccgt tgtatacgac acctaagact cgtgcttgta ccatatctaa
     1141 cagttctttt gctttagtca atgattcttt tcgtgacgta ttttcccgta cgaccaataa
     1201 ggtgccatcc gtttttgaag acatgatctg tgcatctgtt acggcaacga ctggcggcat
     1261 gtcgaagatc actacatcaa atagttgtct tgcttctgct aaaatctgat ccattcttgg
     1321 cgaacttagt agttctgatg gatttggtgg tttaggtcca cttggcagaa tcgacaaatt
     1381 gtcgactggt gttcgttgga tctcatctgc tacacttcct gatgaactaa gaacagtact
     1441 taatccgctt gcgttgttca acttgaacgt tttatagatg acgggcttac gtaagtctgc
     1501 atcgactagc agcacccgtt gaccagattt ggcaaaaaca acggcaatgt ttgctgcagt
     1561 ggtcgatttc ccttctccag gtcctgatga agtcacaaca atcgttttga tttgctgacc
     1621 agctgcggag gcaaactgga tattcgtccg aatcgtacga tattgttcag cgattggtga
     1681 agagggattg gtgagtgtga ccaaactgac tgcatgagtt tgggctgctt tttgtttctg
     1741 tgttcgtgcc attttctttc ctcctagact cttgagcggc ttctgcgtga tggcgctgtt
     1801 ttatcgtttg tagcttttgg ttgtgtctga gctggtcctt ttttacggat accagcattc
     1861 aattctttcg ctgacatttc tggaactgtt cctaagattg ggaatcctaa tgtatctgtc
     1921 acatattttt catctttcac tgtccgatcg agtaattcca ataagaatgc cagacctaca
     1981 cctaccatca ttcctaacat taagccagtc attaaattca atttattatt tggcgaaaca
     2041 ggtgtcgtat tggcaactgc acctgaaatg atcgagattt tatcaacatt catcacatct
     2101 tttgcgttct cttggaaaac ttgtgaagtt gtgttagcga tctgttgagc agccactgca
     2161 ttcgtactta ttgcctgaat cgagaacatt tgtgaatttt ctgcctgatt aactgagatg
     2221 gcatctttga tttgttcagc agtcattttc acattgtctt ctgtttctaa acgatctttg
     2281 acttcattga gcactaaatc actgacgatc atatctttat acgtattgat catttgcagg
     2341 ttcgtattga catcattgac atttgcagtg tctgattgtg gcagtgtcac gatcaattgt
     2401 gcttgtgaac tgtattttgg tgtcaagatg aaaaatgtta cgattcctga gatggccaag
     2461 ccagcaaaca ttgagatgat gattattacc aagtgtttgc gtaatgtttc aaaaatttct
     2521 ttcaagctga ttgtctcttc cattttttct ccactttcct atttattctc tagattcatt
     2581 tgatctttta attctttttt cacacgatca agttcttgat cactgacttt ttgataggag
     2641 atattatctt gcatgaagcc ttctccttgc agctgatctt gcttaatcgt accaaatgcc
     2701 tcccgataat ctaaagcaat ttttttcatt tcatcaaagt tcatgtctgt tttcatattc
     2761 gactctagag cattcaaaac ggattgatag ttgctgacac cgtttagact caatactttt
     2821 ttcgctacgc cttctacgat ctttctttgg cgcccttgtc taccgtaatc accatttggg
     2881 tcttcataac gcatacgaga ataagataat gcctgatccc cgttcaatgt aattttaccg
     2941 attggaaagt catagccatc ttgtgagaaa gtgaggtcat tattgacttc gataccacca
     3001 actgcatcaa cgagttcctt tagccctttc atattgatag agacatagtg atcgattggg
     3061 atgtctacat atttttgtac ggtatccatc gccatggcag ctccgccgaa tgcgtaagcg
     3121 tggttgatct tgtcttttgt gtcacgacct acgatgtcga catacgtatc acgaggaatg
     3181 ctgacgatcg ttgtctgttt gtcttcagga ctgactgtgg caaccatcat cgtatctgat
     3241 cttccttgat cgacccgacc taaatctcca gtatcaatcc ccatcaatag aacagagaaa
     3301 gattcttgtt tgtccagatc aactggtttc gttcgttctc cttgggttcg ttccactgat
     3361 tcgtaagttt tctcaataga atgtgagaga tccagatata attttgctcc tactcctaca
     3421 attgctaata ctaatacgat aagaactgct aaaataatca gaaatatttt tttccctctt
     3481 gattttcttc ttcgtcttgc catagtttcc tccgtttgct aaccttgctt gaatcaacgt
     3541 tttaactgtc aatagcttat attgctatag cccatttgct cagcaacagt ttgtgctttt
     3601 gttgcgtatg ctgcgcttat tttttcattc cggatctgct ctatttgact gagcaccgtc
     3661 aaatattgat cgtaagtagc ggtggcagtc acttgaccat cctttaacat cgtatcgaat
     3721 aactgatcta gttgcgtgac ttgcgcgatc tgaatatcag cgaatcctag atattgcata
     3781 acaaccgttt tccaaggact atctttaaaa aagctcatat tttcacgtac acgagcgaca
     3841 tctgcacttg tcaccttttc tttgatgatg acatcgtcta cagcttgctg ccagtttgat
     3901 acatttttag taaataaatc gttgacagct gtctggatct tcagttttga ttctgcacta
     3961 tctaactgtg tcacgattgc tttcttctca tcttggattg caacagcttc tttaggcaat
     4021 tctgattctt tgatgtcgta atcatctgct gaaagtttga cagaggatag cttatttgac
     4081 aaacttgtga ttgtttcttc tgttatttca gggtttagaa aaattttttc ttttgtacta
     4141 tacagcttgt ctacttcttt tgataaagaa gtcaaggtgt ctttttgatt ggtgatggca
     4201 gattctgcat aattgatccg ttgctgatgt tgccgctgat tattgatatg gtatccaaac
     4261 aaacagatcg caatagcacc tgcacctacc gccacagcag tcaatgactt gacgaatggc
     4321 ttttccatag attcactcct aagtattgat gatagataaa ttaagatata attcacttac
     4381 ataaaaatga cagcttttta
//
LOCUS       DAYYCY010000180         4396 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000180 DAYYCY010000000
VERSION     DAYYCY010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4396
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.181"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..93
                     /locus_tag="ACHAZB_002487"
     CDS             <1..93
                     /locus_tag="ACHAZB_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFM8615109.1"
                     /translation="RGLYKKTRKEGTLFGFSVCTEIKVLLGIPA"
     gene            complement(151..1371)
                     /locus_tag="ACHAZB_002488"
     CDS             complement(151..1371)
                     /locus_tag="ACHAZB_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFM8615110.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1391..2002)
                     /locus_tag="ACHAZB_002489"
     CDS             complement(1391..2002)
                     /locus_tag="ACHAZB_002489"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFM8615111.1"
                     /translation="MKMKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELP
                     LFNKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVD
                     KPVMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLID
                     EEQVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(2045..2977)
                     /locus_tag="ACHAZB_002490"
     CDS             complement(2045..2977)
                     /locus_tag="ACHAZB_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFM8615112.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3398..4258)
                     /locus_tag="ACHAZB_002491"
     CDS             complement(3398..4258)
                     /locus_tag="ACHAZB_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8615113.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT         1257 a          882 c          727 g         1530 t
ORIGIN      
        1 cgaggattat ataagaaaac ccgaaaagaa ggcactctct tcgggttttc ggtctgtact
       61 gaaatcaagg tattattggg aatcccagct taaatcatag ataccgtaag ggactttatt
      121 ctttatttaa aactttgcaa cagaaccttt ttattttaca gaagcactcg aaactgcatc
      181 tactgcagtt tcatcgtgtg tgctggcgct ggttactgca tctgtttttc caccatgaac
      241 agatgcactt gaaaccgcat cttcatttcc aacaagttgt tgacccgttt gttgtaaata
      301 ccactcaatg attggtttga aatcaagaat gtattcattg attccagcat actctccatt
      361 ttcatcatgc attgcttggt aattatgcac aatatatttt tctggcactt gttttggtac
      421 atgaacacgt accgcatctg ttgctccgct tcgcagttgt tgaattaccc actctacatt
      481 tttatacgat ttttctgggt gacagttggc taatggattc cctacctgac cgggcacacg
      541 agcagctaac atctctttcg cttcttggcg caaattataa tataaaaatt ggttattatt
      601 atctgcaaaa gttaattcca tcggcatcga atctaaaaag taattgattt gatccactgt
      661 caataaccca cgatttaatt tgacgtaagt atttccagaa acggcaccga ctgcttcaga
      721 agctttttcc aaccaatgat catctgacat atcaacatcg ggtattgtcg tagcaattgt
      781 tcctgtcgca tgcaaatctt ctggtggaag ctttttaggc cctgctaagg tagacacgac
      841 tttaatgaat tttacaaaac ttgctaatgt actgcctaaa aagtcaacag ttcgtggatc
      901 aatcaagcga tcttcttggt caaatgcttc ttttgctttt cccaataaaa attcatttcc
      961 gggcatcact aatgcattca cgcctggtgc gtccaaaatt tggcgcaagt gtaattgcgc
     1021 gcgtgaagtt ccttgatcgt agtaagaagc acccacaacc attacaggtt tattttcaag
     1081 tggatggatt ttaaatgata gccattcaag tgcacttttc aatcctgctg gaaccgtatg
     1141 attgtgttct ggcgtagcaa taattacccc atcagccatt aagattttgc gatttagctg
     1201 ttgaatagca ggactatttg tttgatcatc atcttgattg aacagaggta aatctttgat
     1261 ttcaatgatt tctaaattaa atagttcatg atattgtttt tggataaatg ttaataattt
     1321 acggttatat gaaagttcag ctgctgatcc gacgatacct attaatttca tctttgtttg
     1381 ctcccctcga ttaaatcgtt tcccatgaaa agctttcggc ttcacgttta tttgcttggt
     1441 gcgcatgttg caattgacta gtgattgtga caaagagtaa gaaatctttg aatagttcac
     1501 gtaattttgc gacttgttct tcatcaatga gtcgtccttg ctcatcaaag gcttggagtg
     1561 aatgacccaa taaaaattct gagcttggca taattcgtgc ctttaactct ggtgaatcca
     1621 atatttggcg caaatgtgct tgtgcgcgtg aagaacctaa cgtgccataa gacgctccgg
     1681 taatcatcac tggtttatcc acaaacggaa aaataccata tgaaagccaa tttaatgcgt
     1741 taatcaatga agcaggaacc gcatggtcat actctggtgt gctgatgatt acgccatctg
     1801 cttgttcaat ttttccagca atttctttaa caacatttgg tagttctgtg ttttcaggtt
     1861 tgttaaatag tggcagctcc ttgatttcga caatttcgat ttctgcctca tttacaaaat
     1921 aatttttcat aaactgtaat aattcacggt ttgttgaccg cgttgaattt gttcctacta
     1981 atccaataaa tttcatcttc attctctttc ctttctaaca tcgatttctg actgttcgca
     2041 cgtactaaat tttttcaaat ccttttgtat acaagaattg attctctttt gttacaactg
     2101 ctccctcgat acccggctgt tcttcaagcg ctttcaacac ttctaaagaa gatgctccaa
     2161 ataaacgtgt agtccagatt tctccatcta cagaagaatc agacaaaatc gtcaggcttg
     2221 ctacgtcatt tttaactgga aagccagttt tcggatcaat gatgtgatga tacactgacg
     2281 aaccattttg tagataacgt tcgtagattc cagaggtaac taaagattgg ttgtttagtt
     2341 taaccaccgc aagcgactct tcacgtgaac ctttaggatt tcgcaaacca atacgccaag
     2401 caccatccga acgtcttggt gattcaccaa aaacaacgat atttcctcct aagttaacta
     2461 aagcaaacgc tgcatttcgc tctcggcaat gtgtaattaa tttatctgca atgtaacctt
     2521 ttgctaacgc ccccaagtca atcatcattc ctggtttggt taaaaaaacg gttttcattt
     2581 tttcatctaa ttgaatttgg tgtggatccg ttagcagaag taactcagtt atctctgctt
     2641 ttgttggaac caatgcatct tcaaatccaa tgtgccattt ttgtacaacc ggaccaatag
     2701 caatattcag ataactattt cttgcacaac tatgtgcttt tcctaatttg atgagctcaa
     2761 aaagttcagg atgtacaatc acagcttgtc gtccagcagc ttgattgatg gccattaatt
     2821 cagaagttgc atcatgtgcg ctgaagcgat gttcataaac gtgcaaccat tcgcctaact
     2881 ctgctagtaa tcgactaggc tcttcatgcc caacttgtaa tgtaattgtt gtccccatca
     2941 tttgccagat tttcgtttct tcgatcacct gattcatttt gtaccttctt tacttgaact
     3001 acggattaca tgagtaatta gtttttattt ttgttactgt tttcacaacc agatgatacg
     3061 cctgctagat ttatttgtca accgatttta tatatttttt tatataataa ggtaatttta
     3121 atataaaatt ttatattgta aaaaaacaaa taagggaaac aaacaacaca tttttgtgtg
     3181 ttgcttattt ccctctaact atcgttttct atttgatggt taacctactt aaaataaata
     3241 ctgcaacgac aaacggagat caagcatgct ttgtaacgta ttttacgata attacgactt
     3301 tattttatta ctctatcttg ctgccatacc cgtctatccc taatcatctt tggtgaattt
     3361 tttcaaagtt agcataacaa aaataagtat tttttcctta gttaatcaat agtatcattt
     3421 ttttcatatg atttaattca tcaattaatt ttttgttttt attaatttta gcaaaagcta
     3481 gtgcatcttg aatcaattcg agcacttctg ccttcgactt atttttagcc tgtgcgttct
     3541 tcgctaattg ggcagcagct cgtgctaagt aataagtcaa atggttatcc gaacaaatat
     3601 cgattgcgta atttagtagt gcatcacttg tttccaaatc accgtcttgc gcaaaaaaaa
     3661 caccacaatg aaagacgata tttaacaccc gccagatatc ttctgttgta cgaatcggat
     3721 acgtataaat tttttcaaac actcgattga aataaacttc tgctttttcc aactcttttt
     3781 ctctagcata gaccattcca atacctgtat atgccaataa acgaaacaac gtgttggctg
     3841 tttctgccat caacaactga tcaaaatgga acaacacatc cgtaattggt gcattcgtaa
     3901 aaatcatttc aaatcctttt agatagtagt aacgtgcaat cagctcagac tcgtcaatta
     3961 ccttgggatc aatggctgct aaaatctgtg tcaattgttc atattcactc gtaatcagtg
     4021 aaaactctgc tttttctagc tgttcatcaa gttgtgtatt gatcacgttc atcgaagaaa
     4081 aaagttcaac taacggaaga ttgattcgat tacataattt gattaaaatt tttaacgaag
     4141 ggacttgacc attgttctca aatttactta atgttgcttg tgtacaaatt ccttcagaca
     4201 cttctttttg agataagttg gcagcctttc ttgccgtaat aaatttttca atgttcatta
     4261 ttttctctcc aatcagacct cacacgaaaa tcatttctgt ctcgatttta gcatgttttc
     4321 tttgggaatg ctttaggttc tgttgcaaag ttttaaataa agaataaagt cccttacggt
     4381 atctatgatt taagct
//
LOCUS       DAYYCY010000181         4389 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000181 DAYYCY010000000
VERSION     DAYYCY010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4389
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.182"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            156..1862
                     /gene="efbA"
                     /locus_tag="ACHAZB_002492"
     CDS             156..1862
                     /gene="efbA"
                     /locus_tag="ACHAZB_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HFM8615114.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2020..2922)
                     /locus_tag="ACHAZB_002493"
     CDS             complement(2020..2922)
                     /locus_tag="ACHAZB_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM8615115.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2919..3611)
                     /locus_tag="ACHAZB_002494"
     CDS             complement(2919..3611)
                     /locus_tag="ACHAZB_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HFM8615116.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            complement(3654..4295)
                     /locus_tag="ACHAZB_002495"
     CDS             complement(3654..4295)
                     /locus_tag="ACHAZB_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbR-like 5'-deoxynucleotidase"
                     /protein_id="HFM8615117.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
BASE COUNT         1346 a          941 c          760 g         1342 t
ORIGIN      
        1 acctaaaaat ttggatttca acgatagaaa cactgttttt tatgtaatta gtccaagatt
       61 tttctagact tttgctttac tattcggcca aattggtttt tcttcttttt ttcgttataa
      121 tgaatatact tatgaaaaga aatgaggaat gaaccatgtc ctttgatggt gtatttaccc
      181 acgcgatggt caacgaattg cgtgaaacgt tactatctgg ccggatctcg aaaatccatc
      241 agccttatga aaatgaaatc gtgctggtga ttcgttcacg cggcaaaaac catcgattat
      301 tattatctgc gcacccaagt tatgctcgtg tacagattac ccaaatcgat tatcagaatc
      361 ctgataatcc gcctaacttc gtgatgatgc ttcgaaaata tttggatggc gctattttag
      421 aagacatcga acaaatcgaa aatgatcgag tgatccattt ccactttgct aaacgaaacg
      481 agttgggtga tctacaaaat atcatattga tcgttgaatt aatgggacgt catagtacga
      541 tcgtcttagt taatcgagaa actggaaaaa tactggatgc gatcaaacac attggaagct
      601 ctcaaaacac ttatcgttct ttgcttcctg gtgtagaata tgttgctccg ccaaaacaag
      661 aggtcttgaa cccatttagc tccgagaaag aaaaaatctt tcagcggctt tcccaaactg
      721 aactagatcc aaaagcaatc caacgtcagt ttcagggaat cggtttcgat atcgctcaag
      781 agctgaccaa aagattactg gaacgtccta atgaaaagat ggttgtatgg gacgaatttt
      841 tctcagccat cagccatcag cctattccta ctttttacga aacggaaaat aaagattttt
      901 tcacaccgat tgcttatcag gtattgtccg aacaggcatc tgctgtaaca gcttatccaa
      961 cactcagtca attattagat agctattatc acgaaaaagc agagaaagat cgggcaaaac
     1021 aacaaggcgg agaactgatt cgaaaaatcg aaaatgaact aaaacgaaat aaaaataaat
     1081 tgaaaaaacg tgaacaaaca ctcaaagaat ccgaaaacgc tgagaattac cgtagaaatg
     1141 gcgaactact tactactttt ctgacacaag taccacgcgg cgcaaaagaa gttgttttac
     1201 ctaattatta tgaagaagat cggccaatca aaatagcact agatccggca ttgacaccaa
     1261 atcaaaatgc acagaaatat ttccatcgat accaaaagct gaagaacgct gttaagctga
     1321 ttggcgaaca aatccaagaa gccaaagatg aaatccaata tcttgaatct gtcttatctc
     1381 aactggaaat tgccggccca atggatatcg aagcaattaa agaagaattg acttcagaag
     1441 gatacctgaa aaagaagaac ttaaaaaaac aaaaacgtaa gaaaccatct cagcctgatc
     1501 aatacttttc ttctgacgga acgctcattc ttgttggtaa aaacaatctt caaaatgacc
     1561 agctgacaat gaagacagct aaaaaaaccg actattggct tcatgcaaaa aatatacccg
     1621 gttcgcacgt catcattaaa agtgacaagc cctcagatga aacaattaca gaagctgccg
     1681 agttagctgc ctatttttca aaataccgat actctgccca agtgccggtt gatcttgtac
     1741 aggtaaagca tatccgaaaa ccaaatggcg ccaagcctgg ttatgttatt tatgaaaacc
     1801 aaaagacgat tatcgtgaca ccagaagaag aaaaaattac agctatgaaa caaaacaggt
     1861 aatcaatgag tgtttctaga atcatatttg cgtactttcc cacaacagca attagttata
     1921 aattgtttgc tacaaaaaga ggagttgtga caaaattctg tcacaacccc tcttttttga
     1981 tcaacggtgt tcaataaaca gcgttctcat ctgcttttct tagctctgct ggtccagtaa
     2041 ccgagtaagc tctttgataa atctttgagc gatttcactg agcggacgtt tcgccgaata
     2101 aatgatataa aattcattta gttcgccttc tgccaaaggg attaaccgca agtgctcgcg
     2161 aatccctcca atcacaatac cagatcctga agcgtaagca tcagtttcaa gaacgatatt
     2221 catcaaagta cctcgatcac tcgtataaat cactgtttct tgatcaggga tctcgataag
     2281 gtcttcataa aaatacggga aattatttcc atcctgtgta aaacgaactt gaggataaga
     2341 taacaattct tctttggtga tctctgcgtg ttcagctaat ggatgtcctt gccttaaaaa
     2401 aatatgcgtt ttgaattttc caatgctgcg gccacttagc tctccttgtt gtaaatatct
     2461 tctgatacca gcttgattat gctcattcat ataaataatg ccgatctcac tgtgctgctc
     2521 tttgacttct tcgatcactc tcgctgttgt cgtctccatg atccgaaatt gtttgatctc
     2581 gtctttgaat tcctcgagta gctgtgcggt caaaggacct aagaaatcat aatgctgggc
     2641 agcgatcgaa aaattgtctc tcttttcact cgttagatag tgagtttcta atgtatcaag
     2701 ctggaccaat attttctcgg cacgatttaa gaagtcttgc ccttcttctg ttaaatatgc
     2761 ccctcgattc gttcgagtaa acagcgtgat ccctaactcg gtttctaatt ctttgatacc
     2821 agctgaaaga cttggctggg agacaaacat tttctttgcc gcttctctga aactaccatt
     2881 gtttgctaca gcgaccacgt atttcatctg ctgaatattc attgctcaaa ctcctcgaaa
     2941 taataaattt ctcttccatt caagggctgg attggtcggt tgttcggtcg agcatactga
     3001 ccatcgaagg ggagaagaaa ctcttggttc agttttccta catgatccat tacaaaccat
     3061 tggattggcc cgctggtagg cggagttgtc aatgagccaa tataatggaa atggcttttt
     3121 tgctccggaa agaacacatc cggatttacc caatgctcat gtttttctaa gttccaactt
     3181 tccccatttt cttgattata cagccaacct tcctgtacca ttagctggta catgacaccc
     3241 acaactagat tctctttttt gctgttcata tgaacaaaat gaaattcaat atcttcctgt
     3301 tttccgttga tgatatgttc agaaggcaag tggaaatgaa tatctgtcaa ataatagcgt
     3361 tctttcttga actctacata actgagttgg tcaaaaggta ctaaatgaat cgtgtttttg
     3421 aattccttgt cagtaaaccg ttgcttttga taatgaaagg aaagcgtgtc tccttctatt
     3481 ttggtttctt cccccttggt caaatgaata ggcgactgca atggaaattc cgcacctctc
     3541 gcaaaccaat cacataaact cgcccaattt tccggacctt tgtctccaga atagccccat
     3601 tcgacatcca tattcttttt caactatact tccccctgca tctcatatca tttttatttt
     3661 tcttctttct ttacaatcgt tgagacggtt tctaaaaacg cctcttttcc ataaaattcc
     3721 tggttcaata gttccggaaa tatctcttta aagaaatact gaacagaagg ccgatgttca
     3781 aagcttttaa tcgtagtcac tgcctcaatg aacatctcct tataaacttt ttccgggtta
     3841 tttttgatga tttcatcaaa agattcataa agaaggtcaa ccttatctga aatggctaat
     3901 agttctcctt ctacggtttc atcttttcct tcgaacaacc gacggcgata aacttcttgg
     3961 aactcagctg gaatctcttg gttgatgaat tcgtccgtca tcttttcttc aactgtttgc
     4021 agcatccctc ttaactcttt gcttgcgtat ttcaccggcg ttttgatatc accaataaaa
     4081 cgctctgtgt agtcatgatt caatgatttt tcatagagca gtttccaatc gatcggttgt
     4141 ccattcgctt cttcaatgtc tcctagaact tgcgcaatag atgctacacg atacgaatgt
     4201 gctgcaactg tatgctctgt atatttaaaa aatccaggtg cccttgtgat cgtctctaaa
     4261 ttgttcaatc ctaaaataaa ttcattcaac cccataaaag ttacctccct ttgagatttc
     4321 tttttccgaa tatcatatca ttaaataact gatattcgca acatttccgc tctttaccat
     4381 ttttgaaac
//
LOCUS       DAYYCY010000182         4388 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000182 DAYYCY010000000
VERSION     DAYYCY010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4388
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.183"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(17..538)
                     /locus_tag="ACHAZB_002496"
     CDS             complement(17..538)
                     /locus_tag="ACHAZB_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HFM8615118.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            737..>4388
                     /gene="nifJ"
                     /locus_tag="ACHAZB_002497"
     CDS             737..>4388
                     /gene="nifJ"
                     /locus_tag="ACHAZB_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFM8615119.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERR"
BASE COUNT         1376 a          827 c          972 g         1213 t
ORIGIN      
        1 cacgctcctt atttttttat tttgtttgga tcaattcttt taattcctct attgaaatag
       61 attcatcaaa gatttcttta cccaagatga cagttggaac aaatcgaata tgtgcttgct
      121 cagcttcgtt gatgacagct tggctcagct gttcgtcttt ttgttctgtc agctttagtt
      181 tatctactgc aaaatccgct acttcttgga gagaaagatg tttccactcg tcttgagtat
      241 cgaatatttg tttgatctct ttgatcgctt tttggccatc gctgattgtg agataccggt
      301 gcatcacgtt ccctcgctgt aagctttctt tttctttatc gaacaatttg atcactcgct
      361 gcaattgccc gctttggact gcttcttcta atagctcata agattcttca aaccattgtc
      421 ggcaatacgg acaagctaag ttgatgaatt cgaccatttt tttaggagca ttcgatgaac
      481 cataaaggat tcctttttgt gtatttgttt tcgttgcatc aatcactgat atatccatgt
      541 taaaacacct ctttcgctta tagtttatac atgtctgaca aaaaaaggca acgatttaga
      601 ctgtttgtct aaaaattaga tgaccaattc aaatagatac atgtaaaata tttttataaa
      661 taaaagaaaa aaatggcatg ttttacgtta taatggaaca gataataaga gagaatatga
      721 acaaggatga tgaatcatgc gaaaaatgaa aacaatggat ggcaatacgg cagccgcata
      781 tatttcttat gcatttaccg agattgctgc aatatatcca ataacaccca gttcgaccat
      841 ggctgaatta gtagatcaat ggtcagcagc aggaaaagaa aacgtattcg gtcaaccagt
      901 aaaagtagta gaaatgcaat ccgaagcagg agcagctggt gtggtccatg gatcattgaa
      961 aactggtgca ctgacaacta catataccgc ttcacaaggg ctgcttttga tgataccgaa
     1021 tatgtacaag attgctgggg aactactgcc aagtgttttc cacgttgcta gtcgggcagt
     1081 gacgacaaat gcgttgaata ttttcggaga tcacggagac gtgatggctg caagacaaac
     1141 aggatttgct atgttagctg aaagcagtgt acaagaagtg atggatttag cacctgttgc
     1201 ccatcttgct tctattgaag gagaagtccc ttttttgaat ttctttgatg gattccgtac
     1261 gagtcacgag attcaaaaaa tcgaagtttt ggaatatgaa gaattagctc ctctgatcaa
     1321 tcaggagaaa ttagcttcat ttcgtaaacg agggatgaat cccaatcatc cgactgttag
     1381 tggtacgaac cagaatccag acatccattt tcaacaacgg gaaacgatca acccctatta
     1441 tgataaatta cctgaaatcg ttcaaaaata tatgaaagag atcaaccgat tacgaggaac
     1501 ttcttatgac ttggtgactt attacggagc agacgatgcg gaagaagtca ttgtttctat
     1561 gggatcagtt gcacagacga ttgaacaaac gattgattac ttgaatgcaa acggacggaa
     1621 aacaggtttt ttgaatatcc atctttatcg tccttttcca gtggaaacat tcgtgcaaaa
     1681 gttgcctcat acagtaaaaa caattgctgt tcttgatcga agtaaagaac caggggcagg
     1741 tggagaacct ctcttattag acgttcaaag tgcgttatat gattctgaac ttcgcccggc
     1801 tgttattggt ggtcgctacg gattggggtc taaagatgtg actcccgacc aaatctcagc
     1861 agtttttgat gaattgaaaa aagacccaag catacgcaaa aaacggttta ccatagggat
     1921 cgtggatgat gtgacttatc aatctttgga accaaaggaa agtctggatt tgacagaacc
     1981 gcaaacattc caggccaagt tttggggatt tggatctgac ggaacagttg gagcaaataa
     2041 atcagctatc aagattattg gagatcacac cgataaatat gcgcaaggct atttttatta
     2101 tgattcgaaa aaatctggcg gtctgactgt ctctcatctt cgttttggag atacgccgat
     2161 acgttcagcg tatcttgtcg agcatgcgga ccttgttgcc tgtcacacac cagcttacct
     2221 gcatagctat gacttggtca aaggattgaa gccaggtggc atttttttat tgaatactct
     2281 ttggagcgac gaacaattag aaacgcatct gcccttaaaa ttaaaacgtt acttggctga
     2341 aaataacatt agattctata cgatcaatgc catgagattg gcccaagaag tcggtttggg
     2401 acgccggatc aatacggcaa tggaaacggc tttttttaaa ttagctgaca tcattccttt
     2461 tgatgaggtg cttccgctgc taaaagaaga agccctgaaa agctatggac ataaatcaat
     2521 ggctatcgta gaaaaaaaca tacaagcaat cgatcggacg accgaactgc ttcatcaagt
     2581 cgcgatccca gaccattgga gaacgttgga gatgcctgaa aaaatcaata aaaggaacac
     2641 tggttatata caagaaatac ttgaaccagt gaatgcacag gaaggaaatc aactctctgt
     2701 tggtacactt gtagcaaatg gaatgacaac tggcgaaatg cctttaggaa cggcttctat
     2761 ggaaaagcgg ggcgtcgctt tagaagtacc tgagtggatt agtgatcgct gtacgatgtg
     2821 taatgaatgt gcctttgttt gtcctcatgc tgccatccgt ccatttttag cagatgaaga
     2881 ggaaatggaa gaagcgccag aaggatttat cgtgagagaa atgcgaggcg cagacggcgt
     2941 aaaataccgt atccaagttt cagtggaaga ctgtacaggt tgtggtctgt gtgtagaggc
     3001 ttgtcccgca aaagaaaaag ctttagtgat gaagccttat gaagaacaga aagaacaagc
     3061 tgtgaattgg gcttttgcaa tgacattaag acaaaaagca aatccagtca aaggaaagaa
     3121 tactgtcttg tctacacaat tcaatcaacc gcttcttgaa ttctcgggcg cttgctcagg
     3181 ctgtggcgaa acaccttatg taaaattatt gacacaaatg ttcggtgatc gaatgatgat
     3241 cgcgaatgcg acaggctgtt cttctatttg gggcggtgct tctcctgctt ctccttatac
     3301 gaccaatgaa tgtggtcaag ggcctgcatg gtcaaattct ttattggaag ataatgctga
     3361 atttggttat ggtatgcttc tagcggcaca gactcgtcgc gaggctttgg caagttcaat
     3421 caaagaagta ttgccaaaag tatcagaaga actacgagaa ttgatggaag actggttagt
     3481 acacatgttt gagagtgaag gaacgcagca aagagcagca aaactgcaag ctgcgatcga
     3541 aagcgaacga tcaattttcc ctgaactgga ggatatatac aagagaaagg atctattcgt
     3601 aaaaaacagc caatggatga ttggtggaga cggatgggct tacgacattg gttttggcgg
     3661 gatcgatcat gtacttgcca gcggagcaga cgtgaatatc cttgttttgg ataacgaagt
     3721 ctattccaat actggcggtc aaacctcaaa agcaacacca gcttcggcaa tcgctaaatt
     3781 ttcggctagt ggaaagtatg cttcaaagaa agatctagga atgatggcga tgacatatgg
     3841 aaatgtttat gtggcgcaaa ttgcttcagg tgccaatcaa atgcaaacga tcaaggcatt
     3901 tgaagaagca gaaaagtatc ctggtccttc tctgattatt gcctatacgc cttgtatcac
     3961 acatggcttg cttggcggaa tgaaagaatc gcttaaagaa gcaaaagaag ctgtttcttc
     4021 tggttattgg tcattatatc gatataaccc tacaatggtc gaaaaaaaca agaacccaat
     4081 gactttggac tacaaaaaac ctgatttctc tgctatgcct gatttcatga ggaaacaagt
     4141 tcgcttttcc tcgctagata acgcaaatcc aaatcttgca aaagctttat ttgaaaagac
     4201 tgtccacgac gctaaaacac gtttttacaa ctatgctgca ctgacgggag aattagaaaa
     4261 aatcaagaaa cgacttgaac cagaggaatc tatcgatgta cccgttaaga ctagaaaaga
     4321 aaaaacgacg gaccttgaag cagaagaaag aagagctgct agaagagctg ccagagcaga
     4381 acgacgta
//
LOCUS       DAYYCY010000183         4344 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000183 DAYYCY010000000
VERSION     DAYYCY010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.184"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(387..1853)
                     /locus_tag="ACHAZB_002498"
     CDS             complement(387..1853)
                     /locus_tag="ACHAZB_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFM8615120.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKKITEPETVVQP"
     gene            complement(2144..4228)
                     /locus_tag="ACHAZB_002499"
     CDS             complement(2144..4228)
                     /locus_tag="ACHAZB_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM8615121.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKKFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TTNIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPLSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKKEDE"
BASE COUNT         1308 a          927 c          697 g         1412 t
ORIGIN      
        1 aataaatggt gttcaatcag ctgaccttca gcattagctc aaaaagcggc aaaatatgag
       61 gagctatttt tccgtttgtt tctctaatac tcaggtcaaa tcgctgattt tacaacctct
      121 tccttaaacg gtgttccaaa gcttgcatct gcggtaataa gcccagccaa acgaaaaata
      181 tgaggggcta tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac
      241 ctctgattca cggtgttcca gaagtagctt cttcgaaaaa caagccgaaa cttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttgt aacaagtatt tctattttaa ggctgaacca ctgtttctgg ttcagtgatt
      421 tttttgtttt tgtctcgacg agcaattgct ggcaagatca tacctaataa gatcaagacg
      481 attggtgtga tgatattcgt aaataattgg aaccaccatg ctttaggatc agcagtataa
      541 tctacttttg gcaccattcc taggatacat gcaaacgcag tgaaggcaaa gcaccaagca
      601 ccaaagataa aaccgatttt tggattttta acaaatttat attcagaatt gaatttctta
      661 taagctttat tcaacatcat atatgccaag aacacccata ggtaacgcat tggcatgaca
      721 actgagttca agttcgtcaa ccattttact aattcattca tatctttgat accaaaaatc
      781 ggcaacaaaa taatgattcc tactagtata cctgtcagaa gataaccatt caccaatgta
      841 cctttacgat tacgtttacg tagccagttt ggtacaaatt ctggatccgc atctgctaac
      901 aagattttta gtggtgcatc aattgaaaag gctaatgcgg agatttgtcc aatcatgttt
      961 gtcaatgcgt aaatgatcat caatgagtta ccgataccat agtaattacc taaaatttga
     1021 aaagcactgt atgccccatt tgccatcaag tctttaggaa tattatcact agcaaacagc
     1081 atacccattg cgactgagcc taatacagca caaatcccta ccattgctgc taagaagatc
     1141 atccctttag gaaattcctt tgcaggattt cttgtagagt tgacataagg ggatattttt
     1201 tccgcccctc caactgcaaa tactagcatc gaaacggttg tgaaataact gaaatcaaat
     1261 gttggaatat atgttttcac ttgattcata tgtggtgtgg caaattccat cgttggattg
     1321 atgaacggtg ccgttacagc taaaagaata aataagatcg acatgacgaa catcgctgtt
     1381 ccggccagtc cgccaatcac tttcaaagta gaaagtcctt ttgttgataa atataaaaag
     1441 gcaagaaata taataagtga aataactgct accatttgta ctgacatcgt atttacgata
     1501 tttccatttc cttggactgc ccaaccacca gcaatcagaa ttgcttgagg cttttgtgcc
     1561 aaataaggaa tatggacgac ccagtaagtc caagcggcat agtatgccag tcgtttatta
     1621 gatgtcgtat tttcgaccca tgaactgacg cctcctttac tatccttgaa ggctgatcct
     1681 aattgtccaa cgatcaatgc gtatggaata aaataaatgg ccaaaatcaa tagccaagag
     1741 gtgacaactg atactccttg ctgagcatag ttattaacta cattccccat gccccaaacc
     1801 atgttaaatg caattaacgc aacagtaaac cagcgtaatt gtttgttgtt cattcgttct
     1861 ctcctttatg ataaaaattt gttatccaga tgtaatcata ttcaattttt aacaaactta
     1921 caagacagtg ttattttatg tgtgtattct tttatttaat acacatcgtt ttttcatatt
     1981 ttttccatca aaaaaataag ttattttctt ttatttcatt gaccttattg aatttcaagc
     2041 tattttgaat tttctgcaaa attaaaaaca ttagttattt tataatccta tctattttct
     2101 ttacattttt cattaagaaa aggatataaa aaaaagagga cgttcattcg tcctcttttt
     2161 ttacatgcaa gcttcgcatg gcattcaaaa cagctaatac tgtcacccca acatctgcaa
     2221 atactgcatc tcccattgat gcaaaaccaa aagcacctaa ggcaagtacc aaaattttta
     2281 tcccaatagc aaagacaatg ttttgtttga cgattttcaa tgttttcttt gctaaatgga
     2341 ttgcttctgc aatcttcgat ggttgatcat tcatgatgac tacatctgct gcttcaattg
     2401 cagcatcgct tcccaatcca cccattgcga ttcctacatc tgctcttgct agcaccggtg
     2461 catcgttgat tccatctccg acaaatgcgg tctttttatt gtttctttgt aagatttcct
     2521 ctaaccgctg cactttatct tcaggaagca attcgctata tatcttgtcc acgcctactt
     2581 gcttcccgat atgatcagca atttttttcg agtcgcccgt caacataact gtgttcttca
     2641 caccagcctg ttttaattga accaatgcgt ctacggcatc ttctttcaag gtatctgcaa
     2701 taacaagata accactgtaa ctttgatcga tcgctagata aagcaatgta ccgatttctt
     2761 gacttaaagg aaaagagatt tcaaatcttt ccatcaattt gtgattacct gctaatactt
     2821 gtttgccatc aacttcagct aagatccctt cacctgcaaa ttcctggata ttagttgttg
     2881 gtaaaagcgg acctgcgtag ccttctaaaa cagattgagc aatcggatgt gtagaaaact
     2941 gttctgcact cgcaatatat tgcatgaatt tctttggatc gatttttgta tcgatcgtct
     3001 ggatcgaaaa gtcacctttc gttaatgtgc cggttttatc gaataccact gtctcggttt
     3061 gcgcaagcaa ctccaagtaa tttgaacctt ttactaatac accgatttta cttgcgcctc
     3121 cgataccgcc aaagaaacta agtggcacgg aaatcactaa tgcacatgga catgagatca
     3181 ctaaaaatgt caatgctcga tagatccatt cagaaaacgc cgctccagaa acaacaagtg
     3241 gaggtaaaac agctaataaa acggccaatc ctacaacgac tggtgtgtaa tagcgcgcga
     3301 atttagtaat gaaattttca gctggtgctt ttttactgct ggcattttct actaattcta
     3361 ataatttgct gatagtagaa ttttcataag atttttctac ttgaactaaa aatggtttat
     3421 ttttattgat aaatccgctt aatactgttt caccaacata tacagattta gggactgatt
     3481 ctcctgtaag tgctgacgta tcgactaatg attctccttc gatgatcgtt ccatctaacg
     3541 gtacacgttc accaggcttc acaagtacaa actgaccgat ctgcacgtct tcagggttca
     3601 cggtcatcaa tccttccgtc gtctgcacgt gagctgtttc tggacggatc gccatcaatt
     3661 cgcgaatcga ttttcttgaa tgactgactg ccaatccttg gaaatactcg ccgatttgat
     3721 aaaatagcat cactgctacg gcttctggat attcaccgat caacatagcg ccaatcgtag
     3781 caacagacat aagaaaattc tcatcaaaga tttttccttt gacgatattc atcactgcct
     3841 tcttcactac atcatatcct atcagcagat aaatcaataa ataccctatc aagcgaattg
     3901 gttcattaaa atcaaataat agcaataaag cgatacctgc aagggaaaat aaaatccgga
     3961 acagcgtatt tctctctttt tcatttccat gttcagatac tttttcctta tctatttctg
     4021 ttactttgac atctggttct aattccttca ctactttttt tgtttcttgt tcaatcgtag
     4081 ccaaatgatc ttcctctgcg tcaatcgtta gttttccaga agtgaagttt accgttgctt
     4141 ccttgactcc attgatcttc tgaactcctt tttcgatttt cattgcacag ttcgcacaat
     4201 ctaaaccttc taatctgtaa cttttcattt taatgacctc ctcgcctcaa catatgaaca
     4261 tctcttcata actaattata aatgaatggt tattcatatg tcaaggtatc cgaaagaaaa
     4321 aatgaaaaac atctgcatcg gaaa
//
LOCUS       DAYYCY010000184         4251 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000184 DAYYCY010000000
VERSION     DAYYCY010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4251
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.185"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..857
                     /locus_tag="ACHAZB_002500"
     CDS             <1..857
                     /locus_tag="ACHAZB_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HFM8615122.1"
                     /translation="NLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTENLSKTI
                     EQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDTI
                     RSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIVY
                     YTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMHI
                     TIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLGL
                     VDNFNNIS"
     gene            874..1308
                     /locus_tag="ACHAZB_002501"
     CDS             874..1308
                     /locus_tag="ACHAZB_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8615123.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            1925..>4251
                     /gene="esp"
                     /locus_tag="ACHAZB_002502"
     CDS             1925..>4251
                     /gene="esp"
                     /locus_tag="ACHAZB_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HFM8615124.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
BASE COUNT         1562 a          589 c          798 g         1302 t
ORIGIN      
        1 aaaatttgac tttgtattct ttaggagaca ttcgagatat tctgatttta gttcattatt
       61 tttttactgg aaaaatagaa gatctattcc atgaaccgtt aattgaatat acagaaaatc
      121 taagtaaaac tatcgagcaa atcaaaatag ataatctgtt atcacaaaat tacgatccag
      181 aaatatatct gtttctttat gaaaataaaa tactggaata tgttaaaaat ggagatattc
      241 gaaatttaga aaacatggtt tttaatctga gtaatggaat aattccaagt gttagtggag
      301 atacgattcg gtctgaaaaa aattattcta tcattgtttt tgaaaagtta gcacaaacgt
      361 ctataacatt aggaatggat ataatagaag cgtatcaaag tagagatgct ttgatacaag
      421 aaaacgaatt agctgtatcg ttacctgagg tcttaaaagt gagggattct gggattgttt
      481 attatactaa ggaaatcgga aaaactaaga tagagcatct ttctccctta atttcttctg
      541 tcgttcaatt catcggctta aatatctata aaagaattac tgtaaaggaa atagctaatt
      601 atttttctgt aagtgaaact aaattaagag aaagctttaa gaatgaaatg catattacga
      661 tttataatta tatctctaag cgaaaaattt ctacggctaa aataatgttg aaatcaaacc
      721 atactatcag tgaagtttct ttaggtttag gattttcaga ttcttcacac ttttcgagag
      781 tattcaaaaa gtacgcaggt gtatctccta agcaatatca attaggttta gtggacaatt
      841 tcaacaacat tagctgagag gttttatttt attatgagac tatatcattt acgtagtttg
      901 gaggagttga ggaaattgga ttttaacaaa aaattttttt gggaaatttc tgatttgagt
      961 tgtttcgtag aatctgagcg tctaagtaat ggtaataata gtgtgttaaa tgaatatgac
     1021 aaagtagtaa ttaataaagt aaaaagttta tgtgaagaag ctagagaatc tatcagtcat
     1081 attaatggta gcaattttaa acaaacatta tttaatctga ttaagctaga tgctaaaata
     1141 tcaagttatt tattcttttt gatacatgat gaatttatgg attatcaaaa attggatcag
     1201 ttaattgaaa gagaaagctg ggaagattat tacgtgggtc taactttttc agaaaagtta
     1261 aacaactaca agttaataga ttataagtat ttgagtgaaa gcaattaatt attcatgtag
     1321 agtactagac actttaaata tggacaattc aattataaag aaaaagaatc aataatttaa
     1381 tgaggctgtg acaaatgtca ttttttgttt cgctaaaaac gagtgaaaaa atgttgataa
     1441 ctccaatcat tcgtttaaga ttctaggaat cattcagaaa tgctgtgaaa ccgatacttt
     1501 ataattataa agaaggtgat ttcaaattcc gaagaaggaa tttgaagtca cctttttact
     1561 tatgtcacag tctcattcag taatgcggga taaggctcct tattccgtta tttgaatact
     1621 ctgaagttta atctaagaaa gggtaatatt ttgaaaacat gcttcatcgc atagaaatga
     1681 acttaatttc agaaagaata catacatatc tttttatatg ttttcagctg ctgaatggtt
     1741 aaaaagcaca aatatccgaa aaaaacatga agaaatgtac tttttttgtt gtaatgttca
     1801 tgaaattctc taagtaaatt agttaacata aggttagtaa gattcctatt ctgtgaataa
     1861 tacgtatcag atttatcttt tattcttact aggaaagtaa ttattaaggg agttgatttg
     1921 aataatggtt agcaagaata ataagagagt atttctagaa aaaacaaaga aacgagtatt
     1981 gaagtacagt atcaaaaaat taagtgttgg ggttgcatct gttttgattg gtgttggtct
     2041 tgtcttggga acaactgaac tagttaaagc acaagatgaa atttctccaa gcagcccact
     2101 tgaaacagcg ataagctccg tacaagtagg tgacaaagta gcttcgggga atacttttca
     2161 ggagaatcct ggttatacga aaaattataa cttttcggat ttacaattta gtcctcaaga
     2221 actaactgga gacacactaa aaggcaatac aattggattt gaagtttacg gaaaacataa
     2281 tattgcagca tcaactaaaa actgggaaat ccgtcttcaa ttagatgaac gattagctaa
     2341 atatgtagaa aaaattcagg tcgatccaaa gaaaggaatt ggatcttcta gacggacctt
     2401 tgtaagaatt aatgattcgc tgggtagacc tacaaatatt tggaaggtca attatattcg
     2461 tgcaagtgat ggattatttg ctggggcaga aacaaccgat acgcaaaccg ctcctaatgg
     2521 ggtaattacg tttgaaaaga gcctagatga aatttttaaa gagataggca tagacaatct
     2581 caagaccgat cgtttaatgt atcgaatcta cttggtaagt catcaagacg atgataaaat
     2641 tgtaccgggt attgatagta cgggctactt tttaacagat tcagatgatt tctataatag
     2701 cttagatgta tctgaaaata atcctgacca gtttaaacat ggatcagtaa gtgcaaaata
     2761 tgagaaacct aatactcaaa caaaagatgg ttcaggatcc acaggtgcta atggagctat
     2821 aatcttagat cataagttaa cgaaaaatta taatttctca tattcatctt ccgcaaaggg
     2881 cacgccttgg tatgctaatt acaaaattga tgaacgatta gttccatatg tagcgggtat
     2941 acagatgcac atggttcagg ctgacaaagt aacatatgat gtttcgtttg aatccggaac
     3001 aaaagtagct gatttagcaa tcgagcgacg ggaaggtcat gaaaattatg gcataggttc
     3061 aataactaat aatgatttaa ctaaacttat cgattttgct aatgcaagtc cacgtccagt
     3121 cgttattcga tatgttttac aattgactaa gccattagat gaaatcttag aagatatgaa
     3181 agcaacagca caagttgaag aaaataaacc atttggtgaa gatttcatct ttgattcttg
     3241 gttgtcggat acgaataaaa aattaatcca gaacacttat ggaacaggtt attattattt
     3301 gcaagatatt gatggtgatg gaaaccctga cgataaagaa gagagcggag acacgaatcc
     3361 atatatcggg aaacctgaat tagaagaagt atatgatgtt gacacaacag ttaaggggaa
     3421 agtattcatc cacgagttag cgggaacagg tcacaaagcc caacttgttg ataaagaagg
     3481 tactgtatta gcagaaaaaa ctatcgctcc aaatgaaaaa gatggggctc caatttcaga
     3541 tactgtagaa tttgaattta cgggtgtaga ttcaagtaaa ttaatcgcga aagatgaatt
     3601 aaaaatccaa atcgtttctc caggttttga taaaccagaa gaaggttcaa ccgttattaa
     3661 ggaatcacca aaagcggttg ataaacaaac cgtggtagtt ggatttaaac cagatgctaa
     3721 agaatcaatt cggaataata aaaacttacc tgaagatgca gagtattcat ggaaaacaga
     3781 gcctgatact tctaacgtta ctgatagtac gaaaggtatt gtaactgtta agatcggaaa
     3841 tcgaactttc gacgtggatg tagagtttgc tgtaaaagct tctcaagcta tggaaaatga
     3901 tgcaacatac gtacctataa caacaactcc agaaacgaca gttcaaagtg gaaaacctac
     3961 atttgataaa ccagatgttc ctttagctaa agatgccttt tcaatttcag atgtctataa
     4021 taagtacttc ggtaatgcaa gtgttgatgc aaacactggt attgttacat tcactccagc
     4081 taaaggtgta ggagaatcgg agccgattac tggaacaatt cctattaaaa ttgtttacca
     4141 agatggttct gtaggcacga ccgatttagc agtaactgta agtaaagata tttatgaaaa
     4201 tccaggagaa aacattcctg caggctacca caaagtaacc ttcaccgcag g
//
LOCUS       DAYYCY010000185         4092 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000185 DAYYCY010000000
VERSION     DAYYCY010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4092
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.187"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1753)
                     /locus_tag="ACHAZB_002503"
     CDS             complement(<1..1753)
                     /locus_tag="ACHAZB_002503"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8615125.1"
                     /translation="MQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKE"
     gene            complement(1713..3466)
                     /locus_tag="ACHAZB_002504"
                     /pseudo
     CDS             complement(1713..3466)
                     /locus_tag="ACHAZB_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(3576..4091)
                     /locus_tag="ACHAZB_002505"
     CDS             complement(3576..4091)
                     /locus_tag="ACHAZB_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HFM8615126.1"
                     /translation="MYFFSIMVSSIGGAFGHSISPGIFGLLSGIIFVYISYNYPRKGI
                     FTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVFGYDRAIPQVASWALMQLG
                     SAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGIWGIISVLVVASLSMIGVL
                     IGRKVLKKYKK"
BASE COUNT         1378 a          783 c          577 g         1354 t
ORIGIN      
        1 cttctttttc atagacgaat ttctcagata gtttagtttt ctttatgaga tttttgtata
       61 tttctgacct tcgatacaac tcattatgag taccttgatc aactagtttt ccttgatcca
      121 tcactacaat tttatttacc ttttcaatag acttcaagcg atgcgatata attagtactg
      181 tcttatcttg tattaaacga tttaaacttt cctgaatacg tttttcattt tccgcatcta
      241 aactggcagt aatttcatct aaaattaaaa ttggcgcatt tttcaaaaaa gcccgtgcta
      301 tggataggcg ttgtcgctct cctccagaaa gtgaacttcc gttttctccg accaatgtat
      361 gataaccgtc aggtagtttt tcaataaagt cttcgcagtt agctaaacga gctgcttttt
      421 tcacttcctc atctgacgct tgactatttc caatacgtat attttccaaa atacttgtat
      481 taaacaaagt tacctcttga aaaacaatag cgattttgct aaacaaagaa gctggagata
      541 cacgtttaat atcataccca tcaattaaaa tacagccttc atcataatca tacagtctag
      601 acactaattt caagatacta gttttcccgg aaccactagc acctactaac gcagtcactt
      661 ctccttgttt agccgtaaaa gagatatgat ctaaaattgg ggtattgtta tcatatgaga
      721 aagagacatc tcgcaactca atgtcaaaag atttcagagg actatctgat ccctcttgga
      781 tactggtttc cttcatagct ctaattcgct gtattttagg agctaaataa aatatttcaa
      841 gcactgcttc tttcatagaa tcgaaggaat cctttatttt tattgcggct agtaagtaac
      901 caacgacgta gagtatagtc acttcacccg ttaacagtaa gtgaacacct actagaatga
      961 ctactgctaa actaacatat gagaaaatag aggataaggc catgactgaa aaagtactta
     1021 attctacctt taaatgaatc ctttcactct cttccatttt tttgaaaagt ctgtcttgaa
     1081 cttttttaga tagattaaag ctattaatct cttgctgcaa ttctatagtt tcttgaaatt
     1141 cttccgagtt ttctctcaaa gtatcgtaat atttagtatt ggctttcgtt tgggatttct
     1201 ttgataacaa gattaagaca aaactaaata acgttggcaa aataacagct aatcccattt
     1261 tgacattacc aaccaaaagc atcactgaaa taaaagggaa aaacagagcc ataccgccgg
     1321 ctttaggtat tgcatgactc atcgcatgct caataccttc aacgtcagac atgatagttt
     1381 gagataaatc tgataaatta tgttttgaaa aataggataa tggtaaattt gacaaatcgt
     1441 ctgcaatttg cactcttaaa tgcgcacttt ctttataagt actattatat aagttttcat
     1501 actccctatc taacaaccaa tacatgaaaa gcaaagtaac aaacgatacg gcaaaataaa
     1561 gccaacgggg ctttgtattt tctaaaacta attcgtctat cagcatcatt ataatgaatg
     1621 ctggtaccat attaatgcag tatacaaaaa aactagaaat agaagcttta gttaaagctt
     1681 ctgcgccctg atcacttaaa gcaaatttgt ttttataaaa acttttcatt gtcaagcctc
     1741 caatcatttg cattctcata attagtgacc aattgtttat acacttgatg tttagccatt
     1801 aattcattat gatttccacg ttctactatt ttcccttctg acaagacaag aatttcatct
     1861 acagatttaa tgctggataa tcgatgagct atcataataa ctgttttttc tttcatcaga
     1921 tgttgaaaag ccttttgtag ttcatactca ttatctggat caatagatgc actggcttca
     1981 tccataataa caatctttgc atcttttaaa attgctctgg caatagctat acgttgtttt
     2041 tctcctcctg acagataaac tcctttacta ccaattaagg tatgctctct ttcgggaaat
     2101 ttttctaaaa tcgattcgca accggctaat cttagcgcgt caaatacttt acttgctggc
     2161 gcttgaggat ttgctataga aacgttatcg taaatactct gattgaatag tttcgtatct
     2221 tgaaagacaa aagcaattgt gttaataatt gtgtcttttg aatagtgatt taaaggctta
     2281 ccaccaattt taatctgtcc cttatccaca ttgtagaaac ctgaaattag tttagctatc
     2341 gtactctttc cgcttcctga ttttcctact aatgcataaa ttcttttttg ttctaaaact
     2401 aagttgaaat tttctaaaac acactcttct ttgtatgaaa agctgacatt ttcaaatgca
     2461 atgtcacaat ttttaatctg tgtctcgttc ccatatttta gttggtcttg cttcatttct
     2521 aaataaaggg agtctagtgt ttcgactgca tatcttgctt caaacaagtg catacttaca
     2581 tacattattc tcatgaaaga aacaaaaata attccactta aaaaaaacaa catcaacaag
     2641 tcaacagcta atatcttggg agtggtaaga gaagttataa aaaaggttat aggaactatc
     2701 aaaatgcaaa ctatgccaaa aaatagccat tgaaaaagaa caaaaggtgt tttcccactc
     2761 aaagaatact tatatgcaaa cttagcataa tcgttaattg ctttattcaa ggctttaaat
     2821 gatgttactt tagcaccaaa tatctttata acttgaattc cgcggatata ttctaccgtt
     2881 tcagagctta acacatctaa cgattcttga tatttttgaa taaaatttgt attctctgtc
     2941 attttcttta acaagaatac accaattact aacatggcga ttagaaccaa tccaatttta
     3001 aagttaatga aaaagcctaa ggcaactgat aaaacaggtg ttactatggc ttgagcacta
     3061 tctggaatca agtgagcaat tgccatatgt gttttagccg cgttgtcatc aattgtcttt
     3121 cgaatatatc ccgaagaatt taaatcaaaa aaacgaaaac ttgcttctgt taaaccgcta
     3181 attccctttt ttctcaaatt agtctctaag cgaaacccaa ggatatgaga aaataagcca
     3241 gaaaaaatat aaaataaagc tctaaagtta accaagcaac agtttgaacc gcataactct
     3301 tagctaaata ttcatctcct acaataatta gagactgaag aaatttgtaa atactgtaat
     3361 atccatagac tgttaatacg acagaaaaat cagaaaataa agtggctaac catccgtagt
     3421 atttcatttt agggacataa ctaaataatt ttttgtatac tttcattttt tcctcccccg
     3481 cattcaattt cttctcttat tcgtttacaa ctgttaatgc tttccgtaat agcgtaaatt
     3541 agagatgtac aaacagataa aaggtaaact acgttctatt ttttgtattt ttttaagact
     3601 tttcttccaa tcaatacacc aatcatagat aaacttgcta caactaacac tgaaatgatt
     3661 ccccatatac ctaccgcata gtggatcatt gcatccattg ttgcagcaga ttgtccttta
     3721 tctatccaat cttgacgaaa acgatctgta aagaaccata taggaatcca ctgtccggca
     3781 gcactgccta attgcattag agcccaagac gcaacttgag gaattgctcg atcatatcca
     3841 aatacagata gaataatatc agctaagatc gccatactaa tcgaactgat aaaccaaggt
     3901 aagtacgccc cgcccattaa cgtaaaaaat aacaataaaa caattgtgta aatagtaaaa
     3961 attccttttc tagggtagtt atagctaata taaacaaata tgattccaga taataatcca
     4021 aaaatcccgg gggatatgct gtgaccaaac gccccaccaa tactactaac cattattgaa
     4081 aaaaagtaaa tt
//
LOCUS       DAYYCY010000186         4066 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.188, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000186 DAYYCY010000000
VERSION     DAYYCY010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.188"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            57..1358
                     /locus_tag="ACHAZB_002506"
     CDS             57..1358
                     /locus_tag="ACHAZB_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8615127.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            1385..1645
                     /locus_tag="ACHAZB_002507"
     CDS             1385..1645
                     /locus_tag="ACHAZB_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFM8615128.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(2644..3879)
                     /locus_tag="ACHAZB_002508"
     CDS             complement(2644..3879)
                     /locus_tag="ACHAZB_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM8615129.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
BASE COUNT         1154 a          661 c          851 g         1400 t
ORIGIN      
        1 tctttgcgtt gtcatgcaat gaatggttca tattttattg aaaaggagtg gttttagtgg
       61 aaaagctgat gaaatgggtc gaagagaaac ttgtcccacc aatggcaaaa attggtacac
      121 agcgtcattt attagcaatt cgtaatgggg ttgtttcaac attgtcattg attttgattg
      181 ggacattttt tatggtcttt atcaatttac cgtttcctgg atggaatgaa tttattgccc
      241 cttattcggc aacgattgtt ttacccttta ggatgacgat gggattgatg gctatttatg
      301 caacttttgt tatgggttcg gatctagcta aatcatatgg attagatagt gtgacaggtg
      361 gtatattatc tttaggaacg ttctttatgt tacaagttcc tgtaaacgtt ttaactcctg
      421 aagaggcacc tcttggctgg gtcttaccta tgagttcttt gggagcatct gggatgtttg
      481 cggggatctt atctatgatt tttgcagtgg aagtatatcg tttctttaaa aatcgaaata
      541 ttactatcaa aatgccagaa caagtgccac ctgctgttgc ccgtagtttt gaggcattga
      601 ttccaggtgc agtcatttta acaattactt ggctgattcg aagcgtcgcg ggctttgatg
      661 taaacgctgc cttattatcg ttattcgagc ctttaaccaa tattttaggg aataatttgc
      721 ttggtgtttt actaccaatg tttttgatcc atttgttgtg gtcatttggt atccatggca
      781 tgagtattgt aggggctgtt gttcgtccaa tgtggctgat catgttggat gagaatgcaa
      841 aggcattggc agatggtaca gctgccaccc aattacccta tatcgctcct gaacagtttt
      901 atcaatggac ggtaacaatt ggtggtgcag gagcaacgat cgtggtatct gttttgttta
      961 tattcttctg taaatcaaaa ttcttgaaag aggttggtcg tttttctatt attcctggta
     1021 tcttcaatat caatgagccc ataatttttg gagctcctat gatgttgaat ccttacatgt
     1081 tcatcccgtt caacttagtt cctttagttt tgactattat cagttatttt gcagtcaaat
     1141 tagaaatggt caatagattt actgttttgc caacatggac attaccagct ccaatcggcg
     1201 gttatctttc agcaggaaat gactggcgtg ttgttatctt gatcatgatc aacacattga
     1261 tagcatttat tatctattat ccatttgtaa aagcttatga taaaaagatg ctgtcagatg
     1321 aacaaagtcg tggagaaaac gaagtccaga ctgtctaagt ttggttttta gaaggtggga
     1381 aacaatgtat tggctgattt tattttttgt ttttatcttt ttattaacgg ctagtcacct
     1441 tatattgaat atgctagcta catatcatat tcaaatcaat cgatggattt gggcactggc
     1501 atcatttttg atcgtgattc ttccaaagat cattgtaccg catatgaatg ttttgttttc
     1561 atgggggaca tatgtacttt gtggaatttt tgctatcaat tttatgatcg agcagcatag
     1621 atggtttgtc acatcgaagc tttaaatgtt ctaaaagtgg ttgtaacaga agtggttagc
     1681 ttcaagaaat aagaagaaat ttacgaaaat tgcttcttaa atttttgtga aatgttggct
     1741 tatttttcgt ttggttgaac ttattaccgc agatgcaagc ttttgaccac cgtttattcg
     1801 gaatatggga ctgtgacaag gcttttgtca cagccctttt ttacataaag attattattg
     1861 ttttaactgt tttggcagaa agaaggagga aaaaatggaa attttatcag caatggattt
     1921 agaattagta gatctactac caaagtgatc aagtcaaata tagatttggg agcttacaac
     1981 catatagtag gggctgcagt atggtgtatg aatggtaata tgtatgatgt gagtgtttat
     2041 tcaaatcatg aaacgtgtat agaaataatt gtgctgggaa tagcaatcag tcatggtgaa
     2101 cgtgactttt agtgtatcgt tgctgacgac atagaacatt ctgacatgat ctactcaata
     2161 tgtggggatt gctggcagtt tatataggag tatgcatcgg attgcgagat cattgtctca
     2221 acagaagagg gaggttgatt atcaaagata aagtggtaaa tcctacattt gatttagtaa
     2281 ttgttgaggg gactagcggc acaaatctct acaaagcgat gcttctagat ctatatgaca
     2341 cattttaaga aaatgtattt gcctaattat tatggtcttt cttttgaaga aacgttgaga
     2401 tgatccaacc agagaaaggt gtcaaagaat ttgactaaga ataaatggca gacgctacct
     2461 atgctcattt ggatagcgtc agatctttta ttttaaggat gattttgtgt tttttagaca
     2521 ccgtaattta agtaactcta acttttcaat ccactattta ctttttttgt attttcactg
     2581 tctgggtctc gaatagcgaa attactgatg agtctctatt aaaaaagctt tttctacttg
     2641 aaactatatt gggtggttgc tcaggaaggt ttcgatttct tgaattgctt gttgtggacc
     2701 atttattgga aaaacggctt gcagcgtgcg tgcctgctga cgaatcttgg gtgaagaaac
     2761 ttcgcgcaga gcaatctgca gttttctttc agtcactggt tttgtgaggg cgattgcgtt
     2821 tccgtatttt acacaatagt caatattaca gctttgctca aattgcatcc ccacaccgat
     2881 aaacggtttg ccggattcac aggcagtttg gacggtccct tccccgccgt ggataaaaga
     2941 aaaatcaatg aattcatgca aaagatgact agacagatat tctgttatta aaagatcttc
     3001 tgagtcagat tgaatgtcat caagcagata gtcaataggt gcaataaccg ctagatccgg
     3061 ttgtttttgt aagaagaaca aaattttgcg taacatacgc ggattgcctg aactacccat
     3121 tgcgaaatag ataatacgtt ttctttcttg ttgctttcgc tgtataaaag ttagcacttc
     3181 ttcaggcaag gaagtactga gcttagcaaa aattggacca acgattcgat aattttccgg
     3241 cagctgttct tcttccaaaa atagtttagg cgttgtgatg agatttagat cagcatccat
     3301 catttgtaaa cttgtttttg gtaaaggaag catttctttt tgtgcagcgt gtttgaaggc
     3361 attggggatc catcgggtat ttactatacg cttttttgtc cagtgccaaa ggggattgct
     3421 taaagggccg aagaaacgta actcgtgggg catcagcgca tgctctgatt gaaaaaaagg
     3481 ttcactcaaa gcatagggtt taacatatac taaaggaatc tttttattgc gggccgataa
     3541 aaaaattgtc acattcgaac ctgtcacaat cagtcgaggc tcaaacttag caatcagact
     3601 ctgttcacta gtgatacgag aggcaaccat ttctttggta aaagggtttt taagtgtacg
     3661 caactggtca aattttatga tttgttcttc ttgttttttc gtaaggatcg gcgctagtaa
     3721 atgatactca aagccacttt ctgtgatcaa aggagcaaaa gttttagaaa agccaaagaa
     3781 tacacagtaa taacggtctt tcatagcttt ggcaacctca atcattcgtg ttgtctcagc
     3841 taaattgaat actgcaggag caaaaagaat tgtcttcatg aaatcctcct tattggaatg
     3901 agtctatccc ttcgtttctt actagtatac taaaaaacac agcgttcgag tagcctatgt
     3961 atattttgaa tattaaactg tctctcgctg ttttcacaac ttctaaattt aaattttgta
     4021 atcaagttga aactccaatt ttcaaaaaaa cacgcaacaa tttttt
//
LOCUS       DAYYCY010000187         4063 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000187 DAYYCY010000000
VERSION     DAYYCY010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4063
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.189"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            35..1459
                     /locus_tag="ACHAZB_002509"
     CDS             35..1459
                     /locus_tag="ACHAZB_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HFM8615130.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(1640..2413)
                     /locus_tag="ACHAZB_002510"
     CDS             complement(1640..2413)
                     /locus_tag="ACHAZB_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFM8615131.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(2410..3315)
                     /locus_tag="ACHAZB_002511"
     CDS             complement(2410..3315)
                     /locus_tag="ACHAZB_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8615132.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(3469..3789)
                     /locus_tag="ACHAZB_002512"
     CDS             complement(3469..3789)
                     /locus_tag="ACHAZB_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HFM8615133.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
BASE COUNT         1222 a          751 c          816 g         1274 t
ORIGIN      
        1 tgataaaagc ggttttattt tggaggatgc ttacatggat acagagcaac agaaaaaagg
       61 aattggttta attccacttg ctgccttagt aattggttca gcgattggag gtggcgtatt
      121 tggtattatg acggacatct ccggatcggc tggcggccca gctattttca gttgggtact
      181 cgtaggaaca gcaatgttga tgctctcctt gtcgatcaat aatatcaata aaaaacgtcc
      241 agatttaaaa ggcgggatct ttagttacgc agaagaaggt tttggccgtt tcgctggttt
      301 tatcagcggt tggggttact ggctcacatg ctggttaggc aacgtagctt ttgcaacatt
      361 gttgatgagc gcattaggat atttcttccc agtatttgaa ggtgggcaaa atctggcatc
      421 gatcgtgatg agtacagtct ttttatgggg atatgcatgg ctagtgaatc ggggagtcga
      481 aaatgctagt ttcgtcaatg ctatcgtcac attctgtaaa ttagtgccat tatttttatt
      541 tattgtagta gctatcagca cgttttcgat ccaacaattc attgataact tttctagcga
      601 cattatattg aatgcgaatg gacagccgat cccgttttcc agtcaagtat tgagttgcat
      661 catggtgatg ctttgggtat ttgttggtat tgaaggagcc tctgtcgtag gttcacgagc
      721 aaaaaaacaa tcagatgttg ggaaagcaac gattctaggg atattcggct tgattattat
      781 ttacgtcttg gtttccatgc ttccatatgg gacaatgacg atgaatcagc tgcaaacgat
      841 caaggcgcag cctgctatgg gctatgtgtt tgaaatgatg gttggtaaat ggggagcagt
      901 cgtgatcaac ggcggtttga tcatttcttt agttggtgtt tggctctcat ggacgatctt
      961 accgattgaa acaatgggaa acatggctga agacggactt ttgcctaaag catggggaaa
     1021 actcaacaaa aatggggcac caacttatgc tatcgtattg acgacgttat gtacgaatgt
     1081 ctttttactt tcattattat tcactgacag tgcttataac tttgcttaca cacttggtac
     1141 agcagctatt ttcttcacgt ggcttttctt aggattgtac caaatgaaac tttcttatca
     1201 gcggaaagaa tgggtccaat ttttagtagg gcttttagca agtgcctttc aagtatgggc
     1261 tatgctagtc gtagccttta atgaagtaat gcttgttctt gttcttttct tgccaggcat
     1321 ttatttttac ctcaaagcac gtaaggagca aaacgagcct gtacaacata tccacttaga
     1381 taagatttta cttataggtg tgacgatggt cggagtgatt gcaatggttt tatttgccac
     1441 tgggatgctt cacgtataga agtataaaag aagtataaag agaggttgtg agcctgtcgt
     1501 ttattcaaaa acatactagg aaaggagctg tgacacaaat gttgtcacag ctcctttttc
     1561 agaataaacg gtgtttaaga gacagccaat cggcaataag gctgaacgtc tctttcacag
     1621 cctctttttt gatgagtcat catacatttt ttcgcttaaa tacgagatat cctaatgcta
     1681 agaacaaaac gatgtaagct aaattaccga atagcatttg attagtagaa agatgagtca
     1741 attgcttcat gatctcttcg tttccaattt ggttttgcag attcaacata ctgatcggat
     1801 tccatttgat ccactcccat ttttggatga gtgcaataag gataccagat atgattgacg
     1861 aagcaaaata aaagacgatt cctgctgaga tggatgcgcc ggaactattg ataaagcaag
     1921 caatcatcaa cactaaactt aaaatcagcc ataaaccgat ataactaccc aaagtataag
     1981 tgaacaagct ttggatcagt gtttgaccag tagaaacctt ctccgtgaag gaaatcgaag
     2041 ggaaaagaat cagtttcatc aacacagtga caataatagc taaaaggtat aaataaacag
     2101 aataaataac taatgtgatc catttgctta ctaatatttc tcctcgagaa tactttcgat
     2161 aaaggagatt tttcaatgta ccatattgtg cttccatcga aatgatcgtg cttgcagctg
     2221 caatcatgat aaagacaatc caagatgtgg cactaaaaag ctgagcagtc atttcaactg
     2281 gatcaatgat cgtcgtgtat tttttggcta gaagtgctgt tcctatgagc aaaacaacta
     2341 atagaacaga taaaacagca gttgactttt tcttgatcaa tttaaataat tcttgtttta
     2401 caagcaatgt catttttctt cttccccctt tactgaatcc agaatttcca atagagaaga
     2461 ttccaaatca ttcgtcagat gctggacgtc tgtcacatgg atattttgtg cagacaatag
     2521 ctggatcacg gttcctaata attcttcgtt ttcttttaaa agcttgattt tatcaggatc
     2581 ttccaaagta tatccagctt ctgtcaagat cgttcgagca gctagatcat ttgttgtaga
     2641 gataacgatg aaatcttgcg ctgtttggtt caattcagcc attgtacatt gtttgatgat
     2701 ctttccttga ttgatcagga tcagctcatc gaccattttt tcaagctcgc tgagtaagtg
     2761 actggagatc aagaaagagg tgccctgagt agccaagtcg gtaatgattt ttcgcacatc
     2821 tcttgttgct tgtggatcca aaccgttcat cggctcatct aaaataacga tagacggatg
     2881 gttcagtaag gcaagagcaa ttcccaattt ttgtttcatt cccaacgagt aggatttcgc
     2941 ttttttgtga atgtactttt ccatcttgag ctgattcacg atagtgtcga tagcagatac
     3001 atcgttggtt tcagagaata acttcagatg atcatagcct gttaagaatg gatagatacc
     3061 cgggtattcg atcaatgctc ctacttgttc caacccttta tgactagagg gagaaatctc
     3121 attttcacca atacagattg ttccatctga gtaatggatc agtcctaaaa tagctttcat
     3181 gatcgttgtt tttccagcgc cattaggacc tactaatcca acgattttgc caggggataa
     3241 gctaaaactg acatcgctga gggcttgttt gtgaccaaag ttcttggata attcttggac
     3301 cgaaagactt ttcaaaaata ttcactcctt ttctatagat agtacaagca tacacctgat
     3361 gagtggagaa gtcgctaggt cattggactt atttcttatt ttttcataaa aaaattgaat
     3421 attaaataaa aaggcttatt ttttctgatc agtacttatt ccaagtgtct agtggccgct
     3481 agtaatcttg atgccaatga tactgatgag tagcatcccc acaaagaacc aagtggaaag
     3541 attcagctta tcatgaaaca agaccacacc aattatgacc gaaccaactg cgccaatccc
     3601 tgtccagata ggataagaga gactcatcgg caaacttttg attgcctttg acaaaaagta
     3661 aaaactagca atcatgccaa tcactgtgta aaaagaataa ttgattttag aaaaaccatc
     3721 actccatttc atcatcgttg accagaatac ttccaaaatt cctgctatga ttaattccat
     3781 ccatgccata cttacattcc tccttaacta taaaaaaata cacaaaaaga ggaaaatcat
     3841 cccttcaaag acctaatgga atgatcatcc tctttatcct acccggtggc agattgtata
     3901 tctttttctg tagtatagca cacctatcca gatatcggca atctcatact ttttagtaaa
     3961 gtgtaccaag cactgacgga tatatcgtta gcggaaaaga agatgaatgc tttgcaattt
     4021 catagaaaga agttgttatt tattaatcat ttaaaaaaaa cag
//
LOCUS       DAYYCY010000188         4016 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000188 DAYYCY010000000
VERSION     DAYYCY010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4016
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.190"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(232..381)
                     /locus_tag="ACHAZB_002513"
     CDS             complement(232..381)
                     /locus_tag="ACHAZB_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8615134.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            843..1055
                     /locus_tag="ACHAZB_002514"
     CDS             843..1055
                     /locus_tag="ACHAZB_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8615135.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(1333..3132)
                     /locus_tag="ACHAZB_002515"
     CDS             complement(1333..3132)
                     /locus_tag="ACHAZB_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HFM8615136.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(3256..3852)
                     /locus_tag="ACHAZB_002516"
     CDS             complement(3256..3852)
                     /locus_tag="ACHAZB_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFM8615137.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1443 a          711 c          688 g         1174 t
ORIGIN      
        1 ttatacagat aataacggaa tattatcatc gaaacagcta acaaaattgt cataaaaaac
       61 gagatggttg atattagaac ttgtgttttt gtttaggtct agagataaag agagacaatt
      121 tccaaatata tgtaatgaat acaaaaagac gtttgtcgcg gattaccgtt acaaacgtct
      181 tttattgatt ttccgagatc tcatcagtcg ccatttggaa tggttcactc cttatctaga
      241 ttcgtttaat gctcggactg cttgttcgat tgtaatacca aacgcaacgt tattttcgtt
      301 ttgtgcatca atcgccataa aacggttaga agttgtatca aaagctacgc ggtattgcat
      361 atttgttttt gtaaacgtca taaaattcat ctcctttgta atgtaattta gttcttgaaa
      421 gtttccttta aactttcgaa cttataccaa gtttagcatc gtacgttttt tgatgcaatt
      481 aaaaagcatt tatcattttc cttatttatt ttttcttaaa tggtttagct attttctttt
      541 aatccgataa gtagcccaat catcaaggtt acaaatacta ttgcttgaat ataaatcata
      601 tgctcacctc tttataaata acttagcaat ataaagaaaa aaacgcacga acaacatagg
      661 aatataaaca aacgtttttg tcacaacgtc taagatcgtg ttaaaaaata gttatctaaa
      721 aagcagaaag atactcaaaa taaaaaataa aaaaatggat aaaaacgttt tcaaatacaa
      781 ggtaatttgt tattataaaa taaagaaaaa acaaaataaa aataaagaaa agagggaatg
      841 aaatggtaaa agcagaagta ggaagatctt atatatgcaa accgatcgga gtatctcatg
      901 aactaatcgg ctgtattgaa cgtacatata tcaacacggc tgtcgtgtat gttgaaagtt
      961 atgaccaacg agactatcaa cttatcaatg aaagcaagta tcgcatgctc gtaaaattct
     1021 gtgatattag cgcacctgct gaattggttt cttaaacagt atttttgaga attcgcaaga
     1081 actcccttat aatataaata aagacagaag ggagcgagaa gatatgccaa aaagaaaaag
     1141 ttcagggaaa atgggaatta ttcttgcagt gatctctgca attcctgcta ttttagctat
     1201 tgttcgtgcg atgaaggaat caagagaaag ttaagcacac attatgagcg tcaaacaaaa
     1261 aacgcatata cctgcggtgg caggttatgc gttttttgtt gtataaagac ttttttcatt
     1321 aacgattttt gattagccga ctcccaaaac aaaatttagc agtttatctg aataagtgat
     1381 ttcttcatct gtttgtttca cactttcatt tagtagtccc atctcatcgg ttatgatacc
     1441 atccacacca taaaacatca tgcgactcat agcatctgaa tcgttgattg tccatacata
     1501 tacttctttt ccgtcttggt gggcagaatt aatgaagtta cggttgatcg tactatattc
     1561 cattgtcaga aaatcagcag gcgtcacagg gggcccaaca atattgaaag gtaagatgta
     1621 gcccacataa aagtttggtt ctgcttcttt taaatctgaa acggtttggt acgtcaaact
     1681 ttgcaaaata tgatggttct ctaagataac tttgcggtat ttatggacaa atcgttcgac
     1741 aaggtcatcc gaatcttttt tagaggtctt gatctcaata agtaattttt ggtttagctt
     1801 atgtgctttt tccaaataag aatcaaaaga acaaacaggg gcttgcactc cgttttcagt
     1861 aactgtaaga tttgtcaatt ctttcaatgt aagatcctgt ggtgttccat ttacccctgt
     1921 taagtgacga agatttgcat catgcatcac tacgaaatga ccatcttttg tttcctgaac
     1981 atccatctct acataatcag gatgatagtc tctgcttgtt ttttcaagtg cttctaaagt
     2041 attttgcgcc ccattttggg tactcacacc tctgtgagag acagtcactg gttttgtctg
     2101 aacagctgaa gtcagataat ccatattgta aagacagacg ccaataccga aaaaaactgc
     2161 aaaaagagtc aaaccagtgt ttttcagcgc agagaaacgc aagttggggg cttgtgggat
     2221 aaaccactta gggatctcag gcaagaagcc ttcgtcatcc atgaaatcaa caatgatata
     2281 aaaaatccct acggtagaca tcacgatatt gaataataaa atcccttgta aaagcgtcat
     2341 agcaaacaca gcgctgatca atgcatagtc tgggaaaaat tgttcaacca tactttgtgc
     2401 caaaataaca acgatatatc ctaaagaaga aaggagtaaa atcgtaccgc caatcacaat
     2461 aaattggccc gtgatggcca aaagacgcga tttggtcaat gaccagcttt cccgaatggc
     2521 tgctcgaaaa ggacgatctc gtagaatcat ttccggtaaa gcaaaaatca gtcgaatccc
     2581 aatatagcca aggaaaatat aaacgagtaa aaaggaactg acgatgatcc aacgattagc
     2641 aaaaataaaa tccataatga aagcggggat ttttattttt gacagtagat cagaattaaa
     2701 acttaatccg ctgatcggta gaatcaataa aaaataagca aggaaaaaca aaaaagtaat
     2761 gggtctcact ttcttcagct gaagtatcgt caagtgtaag agttgcttca aagagattgg
     2821 ttctttcttt ttaataaaaa aaacactcat caaaagaaaa gtaaattcga aataaactaa
     2881 cagtaaaatc gtcatcaaaa ctacgattaa cgagagtaga accccaggat gttgagaaaa
     2941 aattgaagga atattgtcat aagacaaata gtcaattgcc cctcggtgta aaataaaacg
     3001 ggtcgtactg ctcaataagg gtaaacaaat aaataaaata aagccatgca tcagcaagac
     3061 atcacggaaa taagcttgtg ttccttttaa aaagtcccaa gcattaccta agctacgttt
     3121 caaataattc atcctatcac ctgctcttca cattgtagcg caaatctaat gaaaaaggaa
     3181 agggtgcagt gtgttttgca gaccctttcc cagatttaac attgtcaatc aatgactaat
     3241 tttacaaaat aaatattatc caagaagacc acttagatat tgacctccat aaatcaaggc
     3301 catactatat aagaaaatcg acagtggttt agcaatcaaa atgattagtg tatatcgttt
     3361 cactgacatg tttgtcaatc cagccatcaa acataacgca tcgtcaggag caactggaag
     3421 aaaaatagct agagcaaaca gcttctcaaa acggttttga ttatccagcc agccaatata
     3481 tttgttatat gtcttatcgc taatcatgga aagtatgaat ggctttccat aacgacgccc
     3541 tagcagaaaa atgataatcg aaccaatgca gattccaata tagttataga taaatccagc
     3601 ataagggcca aatatcagga caccagctgc agtactgatc cctccaggaa tgattggaat
     3661 aactacttgc aggatttgga tgaagacaaa aataatcggt cctaaaatga tggaatcacc
     3721 tactaatcct cttaacgcat tcaaatcttt gaaaacacct aaattaataa aataaatcgt
     3781 cagcgcgatt gataggccta atccgataat ggatatgaaa tttatcagtt ttcgagaggc
     3841 agcaatattc attattcatc aactccttag aggttctttg ttaactatac tttactagaa
     3901 aagcaactaa ctgcgccatt ttttgatatg acagaaaaca aacaattttg taagttcatc
     3961 ataagtataa aagattgatc tcaaaaaaac atccaactaa agacttattt aataac
//
LOCUS       DAYYCY010000189         4010 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000189 DAYYCY010000000
VERSION     DAYYCY010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4010
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.191"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(120..1625)
                     /locus_tag="ACHAZB_002517"
     CDS             complement(120..1625)
                     /locus_tag="ACHAZB_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8615138.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(1622..2308)
                     /locus_tag="ACHAZB_002518"
     CDS             complement(1622..2308)
                     /locus_tag="ACHAZB_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8615139.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2501..3922)
                     /gene="gndA"
                     /locus_tag="ACHAZB_002519"
     CDS             complement(2501..3922)
                     /gene="gndA"
                     /locus_tag="ACHAZB_002519"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFM8615140.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1101 a          851 c          688 g         1370 t
ORIGIN      
        1 gttattctat ttaattgttc gctagttttt ttataatgac atccctgaat ttctcacttg
       61 cttgtatttt gtaccttatt ctttgttgga tatataaaaa aacaagtaaa gcacatgctt
      121 tacttgtctt ctgcctttgc gataggaata aagattctaa aaattgtccc ttgccctact
      181 acactttctg ctaagatacg tcctttatag ttttcaacca gttgtttagc gatagacaac
      241 cccagaccat ttccaccctt cgttctagct cttgctttat ccactcgata aaaacgattg
      301 aagatcttat taaggtcttc tttgggtatt ccttcgccaa agtcctgaat cgcgatctca
      361 aattcattca gagtggaaga aatagagata tggacttctt ttcgtgtagt tgaatacttt
      421 actgcgttat ccaatatgat aatgattaat tgttcaaagt gattacgata aatttgcagg
      481 gtcacttctt ttgtcaaatc atcatctaaa gtgatcacat agtctggata taatatcttg
      541 aaattattat aaacttgata ggtgacttct tttcctttag ttgtttcatt cgcataatga
      601 acatctactt gttctgctct tgataagtct agcatctctt gaactaagct tttcatacgg
      661 ctgatttctt gcatgctggc gcttaatgat tcttctaata tctcaggatc atctttcccc
      721 caacgattta gcagactaag atgcccttca ataattgcaa caggtgtcct taactcatga
      781 gatacgtctt ccacgaattg ctcttgttgt tcaatataca aacgcatacg gtcaagcatc
      841 tcattgaata tttcagctaa atctgccaat tcatcatttg tgtcgatttc aggcatgtgg
      901 atatctgatt gcggatcttt acgtatagta tccattgtat ctctgagtac cttgagtggt
      961 tttaagaaat aagaagataa aaagaaacct aaaatactac ttaagatcaa agaaacaact
     1021 tctaaaacaa taagagtcaa taatagctta tttcgaatat catagaaaga agataattca
     1081 tagaagcttt gcgcatagcc gatcttttct cttgtacctt tagaatatat aggctggata
     1141 gataaaaagc cagtttttcc ttctagtgta acgatcgttg gaactttacg tgtagtctga
     1201 atcaggtttc gactgttttc atgggtttta aaaatcaagt tctcgtccaa atcataaaca
     1261 taaagatcca ttgatggctg ccctagctca gctataaagg aatcgatctt cattaagttt
     1321 ccttcgacca gtgaagtatg atctacgacc tcatctccgt catttgtcgc tttcgtcaga
     1381 ttacgatacg tatttactaa tgtcagttct tcatccgagt tagccaaacg agaagctact
     1441 tctgaaatcg tacgctctac attgtttctt tcttttgcca caatcagatt gatagatgat
     1501 ttataggtaa tgacagcgaa aattgtaaac acaacgaata tgaagaaaga actcgcaaag
     1561 gcccacttga ttgtcaagga gggccctttc agttctcttt tttccgattt tttcggcttt
     1621 atcacgagcg catcacataa cctgttccgc gtacagtttg gatatagctt tcctccccgg
     1681 gaacgtcaat tttgttgcga agataacgaa tatatacgtc cactacgttt gtttcaactt
     1741 ctgtttcgta tccccacact ttatttagta aaacgtcacg tgctaatacc acgttcacgt
     1801 tttccattaa agtaagcagc aactcgtatt ctctttttgt tagttcaatt acttcatttc
     1861 cgcgacgtac gacacggttt tctttttcga tagtcaaatc gcgataagta atagtagttt
     1921 gtttagccac atttttatcg ccttcaatat cgattcgacg caacaatgca cgaagacgtg
     1981 ctaataattc ttcgatagca aatggtttga cgatgtagtc atctgctccg tggtcaagtc
     2041 cagatacacg atcgatcaca gaatctcttg cagtcatcat aatgattggt gtatttttga
     2101 cttggcgtac acgtctgcag acttctaaac cgttcaattc tgggagcatc agatccaaca
     2161 atatggcatc ccactcatta tttaatgccg catctaaacc agttcggccg ttgtagtgga
     2221 cttctgcgtt atacccttca tgcttcaact ctagctcgac aaatcgtgcc aagttctttt
     2281 catcttcaat gattagaata ttgctcattt attattttat cctttctatt ctactggctt
     2341 tttaaaaact ctatcattat accgataata tgcttaaaaa tctatagtca ttttatcatg
     2401 gatagcaatt ttaaaaaaga gctgaagtct actcataaca atcgacttca gctctttcgt
     2461 cctcttttta ttttctcatt tcctaacagc cgaaagacta ttattcttca tggtaccatg
     2521 agtaatggaa gattccttct ttgtcgacac gctcataggt atgtgcgcca aagtaatcgc
     2581 gttgggcttg gattatattt gcaggcagac gttctgctct gtatgaatcg aagtaagcga
     2641 tggctgatga gaaagtcggt acaggtacgc cagcttggac agccaaggca acgacgtctc
     2701 tgactgattg ctgatatttt ttcgtgattt caacgaagta atcatctagc aataaatttt
     2761 cgatctcagg atttttgtca taggcatccg taattttttg caagaatcga gctcggatga
     2821 tacatccagc gcgccagatt tttgcgattt caccaaatgg caagtcccaa tcaaattctt
     2881 ttgaagcgac acgtaattga gcaaatcctt gggcataact catgatttta ctgaaataca
     2941 acgcttctcg gattttttcg accaattctt ttttgtctcc cgtgaactca aaatcgttcg
     3001 tacgagacaa aatcttgctt gcttgtacgc gttcttcttt ataagcagaa atatagcggg
     3061 caaatacaga ttcagtgatc aatggaagtg gcacacctaa gtcaagcgcg ctttggcttg
     3121 tccatttacc tgttccttta ttcccagctg catcaaggat cacatctact actggtttac
     3181 ctgttccttc atcgtcttta cgtgtcagaa tgtcagcagt gatttctatc aaatagctgt
     3241 ctaattcgcc ttgattccat tctttaaaga tatcagccat ttcttctact gaaaggttta
     3301 aaatattctt cattaagtca taagattcag caatcagttg catatctcca tactcaatac
     3361 cgttgtgtac cattttcaca taatgtccgg ctccatttgg gccaatatat gtgacacatg
     3421 gtgctccgtc ttctgctttt gcagaaattt gctctaagat tggtgcaact agcgcatagg
     3481 cttctttttg tccaccaggc atgattgaag gtccttttaa agctccttct tcaccaccgg
     3541 aaacgcctgt accgataaag ttgatgccag agttggctaa ctcttcatta cgacgcatcg
     3601 tatctttaaa gaatgtattt cctccgtcaa tcaaaatatc gcctttatca aggtgaggaa
     3661 gtaactcttg gattgttgca tctgttgccg gtcctgcttt gaccattagc aagatccgac
     3721 gtggtttctc gatagattct acaaattctt cgattgtata tgttgctttg aaatttttat
     3781 ctgggtgttc ttctactaca gcggttgttt tagcccctgt acgattgaat aaagccactg
     3841 aatagccgcg gctttctatg ttcaaggcta gatttttacc cataacggcc ataccgacaa
     3901 cgccgatttg ttgttttgac atatgaactc ctcctacata cgggatttct ccctaatctc
     3961 ttacattata gcttaacacg gctacattgc aaactaaaga acaacatatt
//
LOCUS       DAYYCY010000190         3825 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000190 DAYYCY010000000
VERSION     DAYYCY010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3825
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.192"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1256)
                     /locus_tag="ACHAZB_002520"
     CDS             complement(<1..1256)
                     /locus_tag="ACHAZB_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFM8615141.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWSNLRQ
                     KIISNNQAELNDSPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIERENPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIIQIELK
                     QVTAKDGFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGVSSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVV"
     gene            complement(1270..2487)
                     /locus_tag="ACHAZB_002521"
     CDS             complement(1270..2487)
                     /locus_tag="ACHAZB_002521"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFM8615142.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLGDMMDVTSVKRIHQSDWTNSG
                     IRFLRARDIVSAAKNEEPSDYLYISEEKYNEYSKNSGKVSQGDLLVTGVGSIGVPLLI
                     TDDNPIYFKDGNIIWFKNEHKIDGNFFYYSFINNKIQKYIRDVAGIGTVGTYTIDSGK
                     KTPISLPTYDEQIKIGSFFKQLDHTIMLHQHELDLLKETKKGFLQKMFPKNGAKVPEV
                     RFPGFTGDWEERKLGEVVVFLNGRAFKQQELLDSGKYRVLRVGNFNTNDRWYYSNLEL
                     EEDKYANKGDLLYLWATSFGPEMWNEERVIYHYHIWKLKFLSKELDKQYLYTWLLTDK
                     EKIKESTNGTTMVHVTKGGIEQRTFQFPPDINEQQKIGAFFKQLDDTIALHQRKLDLL
                     KETKKGFLQKMFV"
     gene            complement(2477..>3825)
                     /locus_tag="ACHAZB_002522"
     CDS             complement(2477..>3825)
                     /locus_tag="ACHAZB_002522"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFM8615143.1"
                     /translation="IVDILGYSISPEYLFNVLADQAKQATFQLNDLNKAFVQLASTYN
                     QFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKKLNDVEVLGHDGDVIGDAYEFLISQF
                     ASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERRFFSVFDPTMGSGSLMLNVRNYLTHP
                     DNVKYHGQELNTTTYNLAKMNLILHGVDAEEMNLRNGDTLNKDWPTDEPYTFDAVVMN
                     PPYSANWSADTTFLDDSRFNRYGKLAPKSKADFAFLLHGFYHLKETGTMAIVLPHGVL
                     FRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGTSIPTTVIVLKKNRQTRDVLFIDASR
                     EFVKGKNQNKLSEENIQKILETYAERKDVEKYAHLATFDEIKENDYNLNIPRYVDTFE
                     EEEPIDMVHVGNDIKKIRQEQQVLEKELLEAISSLQTTPENEAWLQGALEVFKHEQ"
BASE COUNT         1053 a          826 c          648 g         1298 t
ORIGIN      
        1 actacaaaca cgatatgttg ccccattaat ttttggaagc gattggttcc ttttttctct
       61 tcctgtttct ttgcatattt caacgccgca tctagttttt gacgggttgt aatgatcaca
      121 acattcgcat tggcatctgc caacagagtt tcacttaact ctttggcact tcctgttccc
      181 acgatcaagc tattcgcttt ggcattacca gaagaaaccc cagtattaaa ctccgaagca
      241 aatttcgtaa actctgtggt ggtctgatta tctaaatctt tgcggtccac taacatgatc
      301 gttcgatcga tccccgattt tcgtgctaaa agcttagtag atacaaaact agtcaacgtc
      361 tttcctgaac cagtcgcatg ccacacatac cctgattgat gtttatttgc ggcaataaac
      421 aaggcttgaa tcgcatgcac ttgataagga tgtaacacca tgagggtttt attgtcttga
      481 tcttcactga caatcgtata atccgcaatc aagcgatggg catctgggat atttagcact
      541 tgtttgcaaa actcatataa gttttctacc tttttattat ctttcgttcg ccaactaaat
      601 aaaaacttag gatgcaaatc cttcggtaag gcatttgcaa aataccgagt cgtttgttca
      661 ttggaaacca caaacaactg taaggtcgaa aagatattgt tccgaaacat gccttcttca
      721 gcatattttt tgatttgatt aaacgcctga taaaaaccat cttttgcagt tacttgtttt
      781 agctcgattt ggataatcgg taagccattg atcaataagg tgacgtcaaa acgtcgatcc
      841 cgcgcttcta tgttacttcc acgttttgca atttgatgga ccacttcata agtagagatt
      901 cctccaccaa tgtcttgatt ggaataaagg ataagtgaga cagaacccaa ttgcggattt
      961 tcccgttcaa tcgtgatccg agccatccca ttttcacctt ttaaccattt cgcagcatca
     1021 aatggggttt tcgtccgtag caataattcg gttttgatcg tttcaaactc tttatcggtc
     1081 aaaggagaat cattcagttc tgcttgatta tttgagatga ttttttgacg taaattgctc
     1141 caaagatctt cttctgattt tagatccgga cgatacgtcc actgattatg tccttccccg
     1201 aggacacgaa tcaactgttc ttcgacttcc gcttcctcac ggtgactaat tttcattctt
     1261 cttccctccc taaacaaaca tcttttgcaa gaagcctttt ttcgtttcct tcagcaaatc
     1321 taacttacgt tgatgaagag cgatcgtatc atctagctgc ttgaagaagg caccaatttt
     1381 ttgttgttca tttatatccg gtggaaactg aaatgtcctt tgttctatac cacctttagt
     1441 cacatgtacc attgtcgttc cgttagttga ttctttaatt ttctctttat cagtcaatag
     1501 ccaagtatat agatactgtt tgtccaattc cttagataaa aatttaagtt tccaaatatg
     1561 ataatggtag attacacgtt cttcattcca catttcagga ccgaagcttg ttgcccataa
     1621 atataagaga tctcctttat tagcatactt gtcttcctct aattctaagt ttgaataata
     1681 ccatctatca ttagtattaa aattacctac tcttaaaacc ctatattttc cagaatcaag
     1741 taattcttgt tgtttaaaag ctctaccatt caaaaaaact accacttccc ccaacttccg
     1801 ctcttcccaa tctcccgtaa atcctggaaa acgaacttct ggcacttttg ctccgttttt
     1861 agggaacatt ttttgtaaga atcctttttt cgtttccttc agcaaatcta attcgtgctg
     1921 gtgaagcatg atggtgtgat caagttgttt gaaaaatgaa ccgatcttga tctgttcgtc
     1981 ataagttgga agtgagatag gtgtcttttt accactatca attgtatagg ttccgacggt
     2041 tccaatgcct gcaacgtcac ggatatactt ctgaattttg ttatttatga acgagtagta
     2101 aaagaagttt ccgtcaattt tatgctcatt cttgaaccaa atgatgtttc catccttaaa
     2161 gtaaattggg ttatcatcag tgattaataa ggggacacct attgatccga caccagttac
     2221 aagcaagtct ccttgagaaa ctttacctga atttttcgaa tattcattat atttttcttc
     2281 tgaaatgtaa agataatcgg atggttcctc attttttgca gctgagacaa tatcacgagc
     2341 acgtaaaaat ctaatacctg agttagtcca atctgattga tgaattcgct tcacagaagt
     2401 aacatccatc atatctccta acttacgctc ttcccaatct tccgtaaaac caggaaaccg
     2461 gatttctggt cgtgtatcat tgctcatgtt tgaacacctc cagcgcacct tgtaaccacg
     2521 cttcattttc aggtgttgtt tgcagggaag agatagcttc taaaagttct ttttctagca
     2581 cttgctgttc ttgtcgtatc ttcttgatgt catttccgac atgaaccata tcaattggtt
     2641 cttcttcttc gaaagtatcg acatagcgtg ggatatttaa gttatagtca ttttctttga
     2701 tttcatcaaa ggttgccaaa tgagcgtatt tctcgacatc ttttctttca gcgtaggttt
     2761 cgaggatttt ttgaatgttt tcttccgata atttattctg atttttccct ttaacaaatt
     2821 cacgactagc atcaatgaat aatacatctc gagtttgacg attcttcttc aatacaatca
     2881 ccgtcgttgg aatcgatgta ccaaagaaca agttcgcagg cataccgatc acagcataaa
     2941 tactgccgtc ttctaataat ttttgacgaa tgaccccttc tgcagcacca cggaataaaa
     3001 ccccgtgtgg caagacgata gccatagtcc ctgtttcctt caaatgatag aaaccatgaa
     3061 gaagaaacgc aaaatctgct ttggattttg gcgctaattt cccgtagcga ttaaaccgag
     3121 aatcatctaa gaaggtcgta tctgctgacc aattagccga ataaggtgga ttcatcacta
     3181 ctgcatcaaa tgtgtaaggt tcatctgttg gccagtcctt attcaacgta tccccattgc
     3241 ggagattcat ttcttcggca tctaccccat gtaaaatcaa gttcattttt gctagattgt
     3301 atgtcgttgt atttagttct tgcccatgat atttaacgtt atctggatga gttaagtagt
     3361 tacgtacatt taacatcaag gaacctgaac ccatggttgg gtcaaagaca ctaaagaatc
     3421 gtcgttcttt ttgatccaac gtcacgattt gtgccatcat atcagaaacc atatgaggcg
     3481 tgtaaaattc tccggctttc ttccctgctt ctgaagcaaa ttgactgatc agaaattcat
     3541 acgcatcccc gatcacatcc ccatcgtgac ctaaaacttc cacgtcattc aatttcttga
     3601 taacttcggt aatcgtcaca tttcgttgtt gctcatctgt tcctagtttt tttgattgga
     3661 gatccacatc atcaaataac ccattaaatt gattgtaggt agatgccaat tgcacaaagg
     3721 ctttgtttag atcgttcaat tggaacgttg cttgttttgc ttgatccgct aagacgttga
     3781 aaagatactc tggtgagatc gaatacccca aaatatcaac gattg
//
LOCUS       DAYYCY010000191         3749 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000191 DAYYCY010000000
VERSION     DAYYCY010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3749
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.196"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            28..1182
                     /locus_tag="ACHAZB_002523"
     CDS             28..1182
                     /locus_tag="ACHAZB_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-A"
                     /protein_id="HFM8615144.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            1397..2365
                     /locus_tag="ACHAZB_002524"
     CDS             1397..2365
                     /locus_tag="ACHAZB_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFM8615145.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            2358..3389
                     /gene="vanA"
                     /locus_tag="ACHAZB_002525"
     CDS             2358..3389
                     /gene="vanA"
                     /locus_tag="ACHAZB_002525"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFM8615146.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            3395..>3749
                     /locus_tag="ACHAZB_002526"
     CDS             3395..>3749
                     /locus_tag="ACHAZB_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HFM8615147.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSR"
BASE COUNT         1143 a          711 c          896 g          999 t
ORIGIN      
        1 cgaaatatat aaaaacggta tggggggttg gttataaaat tgaaaaataa aaaaaacgac
       61 tattccaaac tagaacgaaa actttacatg tatatcgttg caattgttgt ggtagcaatt
      121 gtattcgtgt tgtatattcg ttcaatgatc cgagggaaac ttggggattg gatcttaagt
      181 attttggaaa acaaatatga cttaaatcac ctggacgcga tgaaattata tcaatattcc
      241 atacggaaca atatagatat ctttatttat gtggcgattg tcattagtat tcttattcta
      301 tgtcgcgtca tgctttcaaa attcgcaaaa tactttgacg agataaatac cggcattgat
      361 gtacttattc agaacgaaga taaacaaatt gagctttctg cggaaatgga tgttatggaa
      421 caaaagctca acacattaaa acggactctg gaaaagcgag agcaggatgc aaagctggcc
      481 gaacaaagaa aaaatgacgt tgttatgtac ttggcgcacg atattaaaac gccccttaca
      541 tccattatcg gttatttgag cctgcttgac gaggctccag acatgccggt agatcaaaag
      601 gcaaagtatg tgcatatcac gttggacaaa gcgtatcgac tcgaacagct aatcgacgag
      661 ttttttgaga ttacacggta taacctacaa acgataacgc taacaaaaac gcacatagac
      721 ctatactata tgctggtgca gatgaccgat gaattttatc ctcagctttc cgcacatgga
      781 aaacaggcgg ttattcacgc ccccgaggat ctgaccgtgt ccggcgaccc tgataaactc
      841 gcgagagtct ttaacaacat tttgaaaaac gccgctgcat acagtgagga taacagcatc
      901 attgacatta ccgcgggcct ctccggggat gtggtgtcaa tcgaattcaa gaacactgga
      961 agcatcccaa aagataagct agctgccata tttgaaaagt tctataggct ggacaatgct
     1021 cgttcttccg atacgggtgg cgcgggactt ggattggcga ttgcaaaaga aattattgtt
     1081 cagcatggag ggcagattta cgcggaaagc aatgataact atacgacgtt tagggtagag
     1141 cttccagcga tgccagactt ggttgataaa aggaggtcct aagagatgta tataattttt
     1201 taggaaaatc tcaaggttat ctttactttt tcttaggaaa ttaacaattt aatattaaga
     1261 aacggctcgt tcttacacgg tagacttaat accgtaagaa cgagccgttt tcgttcttca
     1321 gagaaagatt tgacaagatt accattggca tccccgtttt atttggtgcc tttcacagaa
     1381 agggttggtc ttaattatga ataacatcgg cattactgtt tatggatgtg agcaggatga
     1441 ggcagatgca ttccatgctc tttcgcctcg ctttggcgtt atggcaacga taattaacgc
     1501 caacgtgtcg gaatccaacg ccaaatccgc gcctttcaat caatgtatca gtgtgggaca
     1561 taaatcagag atttccgcct ctattcttct tgcgctgaag agagccggtg tgaaatatat
     1621 ttctacccga agcatcggct gcaatcatat agatacaact gctgctaaga gaatgggcat
     1681 cactgtcgac aatgtggcgt actcgccgga tagcgttgcc gattatacta tgatgctaat
     1741 tcttatggca gtacgcaacg taaaatcgat tgtgcgctct gtggaaaaac atgatttcag
     1801 gttggacagc gaccgtggca aggtactcag cgacatgaca gttggtgtgg tgggaacggg
     1861 ccagataggc aaagcggtta ttgagcggct gcgaggattt ggatgtaaag tgttggctta
     1921 tagtcgcagc cgaagtatag aggtaaacta tgtaccgttt gatgagttgc tgcaaaatag
     1981 cgatatcgtt acgcttcatg tgccgctcaa tacggatacg cactatatta tcagccacga
     2041 acaaatacag agaatgaagc aaggagcatt tcttatcaat actgggcgcg gtccacttgt
     2101 agatacctat gagttggtta aagcattaga aaacgggaaa ctgggcggtg ccgcattgga
     2161 tgtattggaa ggagaggaag agtttttcta ctctgattgc acccaaaaac caattgataa
     2221 tcaattttta cttaaacttc aaagaatgcc taacgtgata atcacaccgc atacggccta
     2281 ttataccgag caagcgttgc gtgataccgt tgaaaaaacc attaaaaact gtttggattt
     2341 tgaaaggaga caggagcatg aatagaataa aagttgcaat actgtttggg ggttgctcag
     2401 aggagcatga cgtatcggta aaatctgcaa tagagatagc cgctaacatt aataaagaaa
     2461 aatacgagcc gttatacatt ggaattacga aatctggtgt atggaaaatg tgcgaaaaac
     2521 cttgcgcgga atgggaaaac gacaattgct attcagctgt actctcgccg gataaaaaaa
     2581 tgcacggatt acttgttaaa aagaaccatg aatatgaaat caaccatgtt gatgtagcat
     2641 tttcagcttt gcatggcaag tcaggtgaag atggatccat acaaggtctg tttgaattgt
     2701 ccggtatccc ttttgtaggc tgcgatattc aaagctcagc aatttgtatg gacaaatcgt
     2761 tgacatacat cgttgcgaaa aatgctggga tagctactcc cgccttttgg gttattaata
     2821 aagatgatag gccggtggca gctacgttta cctatcctgt ttttgttaag ccggcgcgtt
     2881 caggctcatc cttcggtgtg aaaaaagtca atagcgcgga cgaattggac tacgcaattg
     2941 aatcggcaag acaatatgac agcaaaatct taattgagca ggctgtttcg ggctgtgagg
     3001 tcggttgtgc ggtattggga aacagtgccg cgttagttgt tggcgaggtg gaccaaatca
     3061 ggctgcagta cggaatcttt cgtattcatc aggaagtcga gccggaaaaa ggctctgaaa
     3121 acgcagttat aaccgttccc gcagaccttt cagcagagga gcgaggacgg atacaggaaa
     3181 cggcaaaaaa aatatataaa gcgctcggct gtagaggtct agcccgtgtg gatatgtttt
     3241 tacaagataa cggccgcatt gtactgaacg aagtcaatac tctgcccggt ttcacgtcat
     3301 acagtcgtta tccccgtatg atggccgctg caggtattgc acttcccgaa ctgattgacc
     3361 gcttgatcgt attagcgtta aaggggtgat aagcatggaa ataggattta cttttttaga
     3421 tgaaatagta cacggtgttc gttgggacgc taaatatgcc acttgggata atttcaccgg
     3481 aaaaccggtt gacggttatg aagtaaatcg cattgtaggg acatacgagt tggctgaatc
     3541 gcttttgaag gcaaaagaac tggctgctac ccaagggtac ggattgcttc tatgggacgg
     3601 ttaccgtcct aagcgtgctg taaactgttt tatgcaatgg gctgcacagc cggaaaataa
     3661 cctgacaaag gaaagttatt atcccaatat tgaccgaact gagatgattt caaaaggata
     3721 cgtggcttca aaatcaagcc atagccgcg
//
LOCUS       DAYYCY010000192         3744 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.197, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000192 DAYYCY010000000
VERSION     DAYYCY010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3744
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.197"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1269
                     /locus_tag="ACHAZB_002527"
     CDS             <1..1269
                     /locus_tag="ACHAZB_002527"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFM8615148.1"
                     /translation="FQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVT
                     ITEVIKKLNDVDVLEHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVT
                     LDQKERRFFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHG
                     VDAEEMNLRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLA
                     PKSKADFAFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMP
                     ANLFFGTSIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAER
                     KDVEKYAHLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEK
                     KLLEAISSLQTTPENEAWLQGALEVFKHEQ"
     gene            1259..2476
                     /locus_tag="ACHAZB_002528"
     CDS             1259..2476
                     /locus_tag="ACHAZB_002528"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFM8615149.1"
                     /translation="MSNDTQPEIRFPGFTDDWEQRKLGDFVVDYVEKTSVQNQFPMLT
                     SSQQKGIVLQEDYFANRQVTTENNIGYFVLPRGYFTFRSRSDNDVFVFNRNDIIDRGI
                     ISYFYPVFTLKSADSDFFLRRINNGIQRQLSIQAEGTGQHVLSLKKFKNIVAMFPSEE
                     EQQKIGTFFKQLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWE
                     ERKLGELGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIER
                     KKWKPIKDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFE
                     TINLPRYSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLL
                     KETKKSFLQKMFV"
     gene            2489..>3744
                     /locus_tag="ACHAZB_002529"
     CDS             2489..>3744
                     /locus_tag="ACHAZB_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFM8615150.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVV"
BASE COUNT         1292 a          641 c          782 g         1029 t
ORIGIN      
        1 ttccaattga acgatctaaa caaagccttt gtgcaattgg catctaccta caatcaattt
       61 aatgggttat ttgatgatgt ggatctccaa tcaaaaaaac taggaacaga tgagcaacaa
      121 cgaaatgtga cgattaccga agtcatcaag aaattgaatg atgtagatgt tttagaacac
      181 gatggggacg tgatcgggga tgcgtatgag tttctgatca gtcaatttgc ttcagaagca
      241 gggaagaaag ccggagaatt ttacacacct catatggttt ctgatatgat ggcgcaaatc
      301 gtaacgctcg atcaaaagga acgacgattc tttagtgttt ttgatccaac gatgggttca
      361 ggttcattga tgttaaacgt acgtaactac ttaacacacc cagataacgt taaatatcat
      421 gggcaagaac taaatacaac gacatacaat ctagcaaaaa tgaacttgat tttacatggt
      481 gtcgacgccg aagaaatgaa tctccgcaat ggggatacgt tgaataagga ctggccaaca
      541 gatgaacctt acacatttga tgcagtagta atgaacccac cttattcggc taattggtca
      601 gcagatacga ctttcttaga tgattctcgg tttaatcgtt atgggaaatt agcgccaaaa
      661 tccaaagcag attttgcgtt tcttcttcat gggttctatc atttgaagga aacaggtacc
      721 atggccatcg tcttaccaca cggagtttta ttccgtggcg ctgcagaagg ggtcatccgt
      781 caaaaattac tagaagatgg cagtatttat gctgtgatcg gtatgcctgc gaacttgttc
      841 tttgggacat cgattccaac gacggtgatt gtgttgaaga agaatcgtca aactcgagat
      901 gtattattca ttgatgctag tcgtgaattt gttaaaggga aaaatcaaaa taaactatcg
      961 gaagaaaaca ttcaaaaaat cctcgaaacc tacgctgaaa gaaaagatgt cgagaaatac
     1021 gctcatttgg caacctttga tgaaatcaaa gaaaatgact ataacttaaa tatcccacgc
     1081 tatgtagata ctttcgaaga agaagaacca attgatatgg ttcatgtcgg aaatgacatc
     1141 aagaagatac gacaagaaca gcaagtgcta gaaaaaaaac ttttagaagc tatctcttcc
     1201 ctgcaaacaa cacctgaaaa tgaagcgtgg ttacaaggtg cgctggaggt gttcaaacat
     1261 gagcaatgat acacaaccag aaatccgttt cccaggattt acagatgatt gggaacagag
     1321 gaagttgggg gacttcgttg tagattatgt tgaaaagaca agtgtacaaa atcaatttcc
     1381 tatgctaact tcatctcaac aaaaagggat agttttgcaa gaagactatt ttgcaaatcg
     1441 gcaagtaact acagaaaata atattggtta ctttgtacta cctagaggat atttcacgtt
     1501 ccgtagtcga agtgataatg atgtgtttgt gtttaaccgt aatgacatta ttgatagagg
     1561 gattataagc tatttttatc ctgtatttac tttaaaatct gctgattccg acttcttctt
     1621 aagacgtatt aacaatggaa tccaacgaca attatctatt caagcagagg gaacaggtca
     1681 acatgttctg tctctaaaaa agtttaaaaa tatagtagca atgtttccta gtgaggaaga
     1741 acaacaaaaa atcggcacct tcttcaaaca acttgacgac acgatcgctc ttcatcagcg
     1801 taagttagat ttgctgaagg aaacgaaaaa aggattctta caaaaaatgt tccctgaaaa
     1861 cggagcaaaa gtgccagaaa tccggtttcc tggttttacg gaagattggg aagagcgtaa
     1921 gttgggagaa ttaggtaaaa ctcaatcggg aattggattt cctgatgccg agcaaggtgg
     1981 ttctgaaggt actccatttt ttaaagtttc cgacatgaat aacattggta atgagtatga
     2041 aatgagaaat gcaaatcatt atgtatctaa tgagcaaatt gagcgtaaaa aatggaagcc
     2101 aataaaagat gtgccagcga ttatatttgc taaagttggc gcagcaataa tgcttaatag
     2161 aaaacgtttg gtcacaagcc ctttcttaat agataacaat accatggctt atttatttaa
     2221 taatacttgg gatatctatt ttggtaaaat attgtttgaa actataaatt tgccaagata
     2281 ctcccaagta ggggcattgc cttcttataa cagttctgat attgagaata tcagtgttaa
     2341 ggttcctgta aaggatgaac aacaaaaaat cggtactttc ttcaaacagc tagatgatac
     2401 gatcgctctt catcaacgta agttagattt actaaaagaa acgaaaaaaa gcttcttaca
     2461 aaagatgttt gtctaggagg gaagaagaat gaaaattagt caccgtgagg aagcagaagt
     2521 cgaagaacag ttgattcgtg tacttggaga agggcataat cagtggacgt atcgtccgga
     2581 tctaaaatca gaagaagacc tttgggtcaa tttacgccaa aaaattatct caaataatca
     2641 agcagaactg aatgattttc ctttgaccga taaagaattt gaaacgatca aaacggaatt
     2701 actgctgcga acgaaaaccc catttgatgc tgcgaaatgg ttaaaaggtg aaaatgggat
     2761 ggctcggatc acgattgaac gggaagattc gcaattgggt tctgtctcac ttatccttta
     2821 ttccaatcaa gacattggtg gaggaatctc tacttatgaa gtggtccatc aaattgcaaa
     2881 acgtggaagt aacatagaag cacgggatcg acgttttgac gtcaccttat taatcaatgg
     2941 attaccgatt gtccaaatcg agctgaaaca agtaactgca aaagatggtg tttatcaggc
     3001 atttaaccaa atcaaaaaat atgcagaaga aggtatgttt cggaacaata tcttttcgac
     3061 cttacagttg tttgtggttt ccaatgaaca aaccactcgg tattttgcaa atgccttacc
     3121 gaaagacttg caccctaagt ttttatttag ttggcgaacg aaagacaatg aaaaagtaga
     3181 aaacttatat gagttttgta aacaagtgtt aaatatcccg gatgctcatc gtttgattgc
     3241 ggattataca atcgtcagtg aagatcaaga caataaaacc ctcatggtgt tacatcctta
     3301 tcaagtacat gcgattcaag ccttgtttat tgccgcaaat aaacatcaat cagggtatgt
     3361 gtggcatgcg actggttcag gaaagacgtt gactagtttt gtatctacta agcttttagc
     3421 acgaaaatcg gggatcgatc gaacgatcat gttagtggac cgcaaagatt tagataatca
     3481 aaccaccaca gagtttacaa aatttgcttc ggaatttaat actgggattt cttccgggaa
     3541 cgccaaagcg aatagcttga tcgttggaac gggaagtgcc aaagaattaa gtgaaaccct
     3601 gttggcagat gccaatgcaa atgttgtaat cattacaacc cgtcaaaaac tagatgcggc
     3661 gttgaaatat gcaaagaaac aagaagagaa aaaaggaacc aatcgcttcc aaaaattaat
     3721 gggccaacat atcgtgtttg tagt
//
LOCUS       DAYYCY010000193         3733 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000193 DAYYCY010000000
VERSION     DAYYCY010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.198"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(587..1144)
                     /locus_tag="ACHAZB_002530"
     CDS             complement(587..1144)
                     /locus_tag="ACHAZB_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8615151.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(1323..1934)
                     /locus_tag="ACHAZB_002531"
     CDS             complement(1323..1934)
                     /locus_tag="ACHAZB_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8615152.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(2047..2304)
                     /locus_tag="ACHAZB_002532"
     CDS             complement(2047..2304)
                     /locus_tag="ACHAZB_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8615153.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(2901..3179)
                     /locus_tag="ACHAZB_002533"
     CDS             complement(2901..3179)
                     /locus_tag="ACHAZB_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8615154.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(3213..3464)
                     /locus_tag="ACHAZB_002534"
     CDS             complement(3213..3464)
                     /locus_tag="ACHAZB_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8615155.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT         1121 a          770 c          503 g         1339 t
ORIGIN      
        1 ctgtcccagc ctccttattc cgaataaatg gtgtccaaaa gcttgcatct gccagattat
       61 ctcatttctt atgacaagct aagcaaatta tctccaccaa ataatttatt catatttttg
      121 acttgcgcat actaactaat tcttaaattt tcatacaaaa tttctcaatt tcccttaacg
      181 agtaatattt atgtacggtg attctcacta gtatcccaat cactttttaa gttatcccga
      241 tttcaattct acttcaaaaa tagttgcttt tatgtatgct gttagttgat tagatggtca
      301 tttgttgagc taggtcgttt attttaaaca agttgtttct ttagcttata cctatctcgt
      361 ctaccaaacg caagatggca aacacaaatg catacgcatc tgtatttgcc actttgattc
      421 gtataaagct aacaactatt aactcctctc agctaatagt tgctctcttt tttattcaga
      481 ttcatttttg ctgatcacag cctagtattt caatggtttt gaacactaaa ccaaattgtt
      541 ccattacaac attcactggc tgatgaaatg gattagtaga tttcgattat tcaaagcgat
      601 tttctattgg tggatcgtgt tcccaactaa taagacggat tccaaaccta tcgtacagat
      661 cttctaaacc acttataaat tttgcaccta tttcaggtgt tttattgcgt ggatattcta
      721 ttgttacgcc attaaatgga taccaattag gattaggctc tctttttttc tgtacatctc
      781 ccatagaata gttagcactt tctgggacat aatatattct tcgaccatcc tctaattcat
      841 ctaataagat atttcgtgat cgtctatcac ttgctaaatg tagacgccac gtgcgatacc
      901 atgccatgct acctgcatct gcagaaaatc catcaaataa tgcaacaatt gtatcttcta
      961 tcacaatcct ttccccatct acttcatatt ctaatctaaa atcaaactcc cctcgggtaa
     1021 tttctggtct tggtggctta ggacccaata ttattctact taaaaccgca ttcgttccca
     1081 gatgaaacac agctataaca acaataatga ttaatatttt gtttctattt gataaatttc
     1141 tcattattcg cacatcacct ctacattctc acatattagt tcgtaatact cctttaatat
     1201 caaaatatct actggtcgta ataatattcg ctatgaattc ctactactcg tataagaaag
     1261 gccaaatcat cagcttttac ttgctgatga tttggcttgt ttctatttat cgtaattgtt
     1321 ttctaatcac caaaattgtt aacaatcggt tcagatattt cccagttgat taaggtaatt
     1381 ccgtattgtg tcaaaacttc ttgcgcttct ccgaaattaa gtacagttct tatgtatcca
     1441 ctttctccta atataaagtc tacctctctt ttaaaacctt cctcgagatt tctaagggct
     1501 atatgtggtt ttaatgacat tgattgtaca ttttccccca tataatattg cgcacttcct
     1561 cgactaaaat agattcctag atcgtcccca aaaggtagat cttctctacc actagctaat
     1621 ctacttctcc atcttcgttc tcttttcata gaacctgcgc tgaactcaca cccgttaaac
     1681 tcagctatga cagtatcttc aattatgtta atctgtccat ctagttcgta ttcaatccta
     1741 aatggaaatt ctccataggt gatttccggt tcgggtattt taggtgagat taatgcaaag
     1801 aaaggaaata ttaagcttgg caacatcaaa aaaactcctc ctatgatcac aattcccgat
     1861 gtaatccaaa atataatttg tacaattctc attgatccat tgtattccat tttattcccc
     1921 ctattactat tcatatccgc ctatctatat cgatcttcca atgcagagat aattgcttcc
     1981 ataccttgat tttttactga gttacctatg cgagatatca ttctccacta aggtgcatca
     2041 tattgtctat ctaacatgat taattctcca ataggtgtat actcctccaa aaacaacatt
     2101 taatgcatct ccctcgacaa cataagctac catgtttgct gtatatgtgt ttgagtctaa
     2161 ttcataagca gataaattta ctgtcgctgg attaaatata atcgcattcg tatttatagc
     2221 tactgaattt gctgcttcag caccaccttt tgaatgtccg atcattgtga tttcaatagt
     2281 aggatttacg tggacagccc tcaccgttta ccctgaccat tatagaaatc gcttactttt
     2341 ttgaactacg caacaaagga tacaatgctc ctaacttcac tgtctccatt tttgtccact
     2401 aaggaatata aaaaataggc attaattcat ttaattcggt cacaacacgt taccttttaa
     2461 atcattatac tgtatctcat acattattat caataaacta ttataaaaat gcaaaaaatg
     2521 ctttatcaat ctattttgta attggtcata atatcattga aatcataccc tgtatcgatt
     2581 tttcctatga atctcgtcta tttgtgattg acgaaaaaga ggcaagacag acactggaat
     2641 ggtaatttta cccactattt tgctccaact atttgtaatc gtacccactt ttgaaatacc
     2701 gagatgatca ttttaattga tatgctgatt cccaagcaaa accccaagaa attaaatgct
     2761 ctatcgcatc tagcttcttg ggttttgctt atataaattc caacatttaa attttttatg
     2821 ctgttcggtc tcagaaatgc cgtttttgct tatatcacaa tatcaagatg tcaaattatt
     2881 cgtgttgccg atgactttga ttaatttttt tcaaattgtt tattaagact aatgctgtct
     2941 ttttgcagat accaatgttc gaacttttta acattatcat atgatcctgc aagttttaac
     3001 ttcttaccat tagatgttat caaagttata attaaccttg cctttttccc attaggatct
     3061 tttattttat gaattttctg caggtaatct ctcctaatgg cagctatttt tacctcatca
     3121 gtgctgataa accattgatc atccacatac atactgcctg ttatgttgtg tttttccatt
     3181 tcttccgaga acgaaaagtt taaggctcgt tcttacttcc aaagttgaat gagtccatgg
     3241 attggactta gtgtgagaat tgagaacaac aataagaatc catatcctcc cgcaaacatc
     3301 cctaaggcta tactgtctct ccatccataa gtaggattag aagagaaaag agtcaccgtt
     3361 tgtaataata tgaaaaggaa taatccagca caaacaccaa agataacaac tccaaacatt
     3421 gaatttttat acaatgacca tttatttata ggcttttctt tcattttact tccccctact
     3481 gttctgttca cacggaatga attcaaagtt cataagccct cctctttgaa catatgaata
     3541 cgacttgcta actatacttt aaaaaagctt gttttaagcg gttataggcg agttgtaatc
     3601 tctattcatc tcattagcaa ataacttgcc gaaaagtcta ctgaaattaa agcagatagt
     3661 ggaagaatta tttagccagg atagacacta tttgtgaaac acatgtcttt acttttgaaa
     3721 ttctatgatg aaa
//
LOCUS       DAYYCY010000194         3728 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000194 DAYYCY010000000
VERSION     DAYYCY010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3728
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.199"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            24..974
                     /gene="yidC"
                     /locus_tag="ACHAZB_002535"
     CDS             24..974
                     /gene="yidC"
                     /locus_tag="ACHAZB_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFM8615156.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVVIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPANNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(1020..1421)
                     /locus_tag="ACHAZB_002536"
     CDS             complement(1020..1421)
                     /locus_tag="ACHAZB_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8615157.1"
                     /translation="MSRQLVLISHGKFCEELKKSTEMIMGPQEDIYTLPLLPEDGTET
                     YKQKFLTVTDSLNDFVVLCDLLGGTPANIISKLIMEGQQITLYAGMNLPMVIEFINSQ
                     MIGSEPEFVRAGCESIVSVNELINGIAEEEE"
     gene            complement(1435..2244)
                     /locus_tag="ACHAZB_002537"
     CDS             complement(1435..2244)
                     /locus_tag="ACHAZB_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8615158.1"
                     /translation="MTKSNYKLTKEDFKQINRRSLFTFQLGWNYERMQGSGYLYTILP
                     QLRKIYGDNTPELKEMMKTHTQFFNTSNFFNTIITGIDLAMEEKEGIASKETVSGLKA
                     GLMGSFAAIGDSVFAALIPTIFGALAANMATDGNPIGVFIWIFAQIVVMVFRWKQLEF
                     AYKEGISLVTTMQSRLTALTDAATLLGVFMVGALVATMINVKFSWAPTIGDVTLNMQN
                     YIDMILPKILPAGIVGGVYWLLGKKNMTPTRAIFIVLIVCVALSALGVISK"
     gene            complement(2231..3121)
                     /locus_tag="ACHAZB_002538"
     CDS             complement(2231..3121)
                     /locus_tag="ACHAZB_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM8615159.1"
                     /translation="MDIQWWQILLLTLYAGYQILDELQIYSSMSAPVFAGLYAGLVMG
                     DLNAGLIIGGSMQLTVLGVGTFGGASKIDANSGTILATAFSISLGMNPEQAIAAIAVP
                     VASLMIQLDILARFANTYFAHRIDRHVEDMNYQAIERNFLMGALPWSLSRMIPVFLAL
                     AFGGGLVENVVSVLNGNLKWLGDGLSVAGAVLPAVGFAILLRYLPVKKHLSYLILGFT
                     ITALLATLFSNVQLLGTSVASVVKDLPATFNSLPMLAVALIGLALAMISYKNDQNKDH
                     QPLASEPSHSVEGEIEDDEI"
     gene            complement(3134..3613)
                     /locus_tag="ACHAZB_002539"
                     /pseudo
     CDS             complement(3134..3613)
                     /locus_tag="ACHAZB_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632653.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
BASE COUNT         1169 a          808 c          630 g         1121 t
ORIGIN      
        1 aattatagaa gaggaagtat tctatgaata aatggaaaaa ctggttattg ggatccggac
       61 ttgtcagtat cctgctcttt ttatctggct gtgtgcggat ggattcaaac ggaaaccctg
      121 acacttcggg aatcgtttat cgagtactcg tccatcctat gggccaagcg attacatatc
      181 tagttgagaa tttcaattgg tcttatggct gggcagttat tgcaatgact gtgatcgtcc
      241 gtatcatcat cttgccacta ggtattagtc aatcgaaaaa aacaatgatc caatctgaaa
      301 aaatgcaagc gttaaaacca caagttgaag cagcccaaca aaaattgaaa gctgcaacaa
      361 cacgtgaaga acaaatggca gcacaagcag aaatgcagca agtataccgc gaaaacggat
      421 tgagtatgac cggcggaatc ggctgtctgc ctctattgat ccaaatgccg atcttctctg
      481 ccttgtactt tacggcacgt tatacagaag gtattcggga atcatctttc tacggcatcg
      541 atttaggctc accaagtatg gtacttgttg tcattgcagg ggtcgcctat ctgcttcaag
      601 gatacatctc aacgatcggt attcctgaag aacagaaaaa gacgatgcgt acgatgctaa
      661 tcgtctctcc tgctatgatc gtcttcatgt cgatcagtgc gccagccggt gttacacttt
      721 actgggtagt cggtggtgtg ttcagctgtc tgcaaacatt catcacaaac gttatgatga
      781 aaccacgttt gaaagcacaa gtggcagaag agttgaaaca aaacccgcca aaacaagtcg
      841 tcactccaag aaaagacgtg acagaatcag caaagccagc taacaacaaa caagcaacaa
      901 aacaaatcaa acaaccaaaa aacagtacaa acaacaaagg ccgcaacgca ggaaagcagc
      961 agaagcggaa ataaaaacat aggacaaaaa agatgagagc taagccctca tcttttttgt
     1021 tactcttcct cttctgctat tccattaatt aattcgttga cagagacaat gctttcacat
     1081 cctgcacgaa caaactcggg ctctgaacca atcatttgtg agttgataaa ttcaatgacc
     1141 atcggaagat tcatcccagc ataaagagta atctgttgtc cttccattat tagtttagaa
     1201 atgatatttg caggtgtacc acctaaaaga tcgcatagta ccacaaaatc atttaacgaa
     1261 tctgtgacag tgagaaactt ttgtttgtat gtttcagtgc catcttctgg taataaaggt
     1321 aacgtgtaga tatcctcttg aggccccatg atcatttctg tacttttttt caattcctca
     1381 caaaattttc cgtgactaat caataccaat tgtctactca tttttcgctc tcccttattt
     1441 cgaaatcaca cccaatgcag ataaagcaac acacacaatt aatacgataa aaatcgctcg
     1501 agtaggagtc atgtttttct ttcctaataa ccaataaaca ccaccgacga tcccagcagg
     1561 taaaatttta ggtaagatca tatcgatata gttttgcata ttcaatgtca catcgccaat
     1621 cgttggtgcc catgaaaatt taacattgat cattgtcgca actaatgccc ctaccatgaa
     1681 tacccctaat aaagtggctg cgtccgttaa tgcagttaag cgagattgca tagtagtaac
     1741 taaggaaatt ccttctttgt atgcaaattc aagttgcttc caacgaaaaa ccatgacaac
     1801 gatctgagca aaaatccata taaacacacc tatcggattt ccatcagttg ccatattcgc
     1861 cgccaaagcc ccaaaaatcg ttggaatcaa agcagcaaat acggaatctc caatcgctgc
     1921 aaaggaaccc atcaatccag ctttcagccc cgatacggtt tcctttgaag caattccctc
     1981 tttttcttcc attgcaagat caatcccagt aatgattgta ttaaaaaaat tagaagtatt
     2041 aaaaaattgc gtatgtgttt tcatcatctc ttttaattct ggtgtattgt ctccatatat
     2101 ttttcttagt tgtggcaaga tagtatataa atagccagaa ccctgcattc tctcatagtt
     2161 ccaacctaat tgaaaagtga acaaacttct tcgattgatt tgcttaaaat cttcttttgt
     2221 taatttgtaa ttagatttcg tcatcttcga tttccccctc aacagaatgt gatggctcac
     2281 tagctaatgg ttgatggtct ttgttttgat cattcttata actgatcatt gctagtgcta
     2341 gaccaatcaa tgctacagca agcatcggca gcgaattgaa agttgccgga agatctttaa
     2401 caacgcttgc cacacttgtt cctaataatt gaacattaga gaataacgtt gctaataatg
     2461 ccgtgatcgt aaatcctaaa ataagataag atagatgttt ttttactggt aaatagcgca
     2521 gaagaatggc aaacccaaca gctggtaaaa cagctcctgc tactgaaagt ccatcgccta
     2581 gccattttag attcccgttt aaaacagaaa caacattttc aactaatcca ccaccaaatg
     2641 ctaaagctaa aaatacagga atcatacgag agagcgacca aggtaaagct cccattaaga
     2701 aattgcgttc gattgcttga taattcatgt cttctacgtg acggtcaata cgatgggcaa
     2761 agtatgtgtt agcgaatcta gctaaaatat ccaattggat cattaagctt gctacaggca
     2821 ctgcaatagc agcaatcgct tgttctggat tcattccaag agaaatcgag aaggcagttg
     2881 ctaaaatcgt cccagagttg gcatctattt ttgatgcgcc tccaaacgtc cccactccta
     2941 aaactgtcaa ctgcatactg ccaccaataa tcaaacctgc gtttaaatca cccattacta
     3001 aacctgcata taaccctgca aacactggcg cactcattga agaatagatc tgtaattcat
     3061 ctaaaatttg ataacctgca tataatgtta ataataagat ctgccaccat tgaatatcca
     3121 tggatcattc ctcctatttg attaaaagat tcataaagtc ttctgcttta tcgtttggta
     3181 ccatttgagc aattaaatgt ataccaagct gatctaattt tttaaatact tcgatatctg
     3241 catctaccac attgattgat tttgtaatgg accgtgtttc attcgtttgt gacatattcc
     3301 ccacatttac ttctttgata ggaactcctt tttctactaa agataagatt cgatctggct
     3361 tttttgctac gatcaatact cgttgagagt catatttgcc agctaaaata tttgttgctg
     3421 ctttttcgat tggcaaaatg cttaatttta cccctgctgg agtcgctaac ttcaatccac
     3481 ttttttcaat tgtgtttgtg gctacctcat catccacgac catgattcga ctaatattca
     3541 gtttcgttgt ccaaagattt gctacttatc catgtatcaa acgtccatcg attcttacac
     3601 caataattgc catcatgcat tctccttatc tattttttct atttattgtt caattaaaac
     3661 tgtgacatca tcaaatatcc cttctgtttc aaagataacg ttttcatttt ttctttctta
     3721 tatttcat
//
LOCUS       DAYYCY010000195         3651 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000195 DAYYCY010000000
VERSION     DAYYCY010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3651
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.200"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..156)
                     /locus_tag="ACHAZB_002540"
     CDS             complement(<1..156)
                     /locus_tag="ACHAZB_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFM8615160.1"
                     /translation="MDFQDYEKIFESYPSILKITDLTKMLNIGKNKAYNLLKDRSIEN
                     IKIGARYK"
     gene            232..304
                     /locus_tag="ACHAZB_002541"
     tRNA            232..304
                     /locus_tag="ACHAZB_002541"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:265..267,aa:Lys,seq:ctt)
     gene            466..687
                     /locus_tag="ACHAZB_002542"
     CDS             466..687
                     /locus_tag="ACHAZB_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8615161.1"
                     /translation="MRNEIANITIIRETLQNHTANEISKHTGLNLSTIKKLKSGERSI
                     EKLNLHDAICLTEFGLKNNRKNVEINIWK"
     gene            complement(1011..1256)
                     /locus_tag="ACHAZB_002543"
     CDS             complement(1011..1256)
                     /locus_tag="ACHAZB_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8615162.1"
                     /translation="MVDNPKKRQYPLVPLSIIQSALEEDPISIKMILKHYHSYIAKLC
                     LTNGFNEAGQFITYVDEYMLRQLEIKLIEAILKFKIN"
     gene            complement(1250..1651)
                     /locus_tag="ACHAZB_002544"
     CDS             complement(1250..1651)
                     /locus_tag="ACHAZB_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFM8615163.1"
                     /translation="MQQNYNEKSIQHQFDAYCKKVLRNETKSIRKRNINITDNEEPLT
                     NSNEGKYSQTHFDEQDVYFLFGMEILISDQKLSAAIDHLSEIRRKVILLYYFAGFNDT
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            complement(1823..3631)
                     /gene="uidA"
                     /locus_tag="ACHAZB_002545"
     CDS             complement(1823..3631)
                     /gene="uidA"
                     /locus_tag="ACHAZB_002545"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFM8615164.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPMVEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVILPKKLLEERLVLRFGSATHEAKVFLNG
                     KLINQHKGGFTPFESEINQFIKDGSNRITVAVNNVIDDSTLPVGILKETIQEDGDIKK
                     ENLVNFDFFNYAGINRPVKLYTTPKDYIKDVTINTKIIDSDANVEFNIDTEWTGEILI
                     AIYDEEAQLIAEAKGKEGVIQLKNPTLWEPLNAYLYSMKVSLINENKVIDTYTEEFGI
                     RSIQVSNGKFLINNKPFYFKGFGKHEDTYINGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHDTWKEIRTENAHKM
                     AIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDICKVGDIVDVLCLNRYYGWYVSGGDLKHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGLHDTVPTMFTEEYQVGYYEANHEVIDKCDNFVGEQVWNFADFETSQGILR
                     VQGNKKGIFTRDRKPKMIAHYLKKRWSSIPNFSYKK"
BASE COUNT         1140 a          673 c          489 g         1349 t
ORIGIN      
        1 tttgtacctt gctccaattt ttatattttc tattgatcta tctttcagca aattataagc
       61 tttatttttt ccaatattta acattttagt taaatcagta attttcaata tcgatggata
      121 actttcaaat attttttcat aatcttgaaa atccataaaa ttcactctca attctgataa
      181 taagtattgc acttttttat tttaggtgtt agaataacaa agtattaatt tgggctgtta
      241 gctcagttgg tagagcagat gactcttaat catcgggtcg cgggttcgag cccctcacag
      301 cccattgggt gccaaaccct taaatacagt gtaaacgttg gtgcttagca tttagaaaac
      361 gtatatactg tatttttttg ttttcaaaag agactgtagt accccttttt ttcactaaaa
      421 gcccactgta gtcattgata tttttgataa gaaaggaaca taattatgcg taatgaaatt
      481 gctaatataa caataatccg agaaacttta caaaaccata ctgcaaatga gatctctaaa
      541 catacaggat taaatttgag tactataaaa aaactaaaaa gtggagaacg ttcaatagaa
      601 aaactaaatt tacatgatgc tatttgcctc actgaatttg gtcttaaaaa taatagaaaa
      661 aatgttgaaa tcaacatatg gaaataattc ttaatcagct gcttttgtca aaagcacata
      721 ggaatttcac ctctctccaa gtttacggcg agccttacgg aagtatcatt atcccaactt
      781 caaatcatcg ctttttattg actataaaaa ttattttaga atgtttatcg ctccttcttt
      841 acagtaagaa atcatggcgc aactaaaata tgctccttga agtgttaacg tactgataga
      901 atgtatttaa atttcaatga tcaataggtt ttctgtttaa aagaatctac taatccttta
      961 actattagaa gattctttta aacagaataa gtggaaaggg gactttttag ttagttaatt
     1021 ttaaatttta agatcgcttc tatcaattta atttctagct gtcttagcat atactcatct
     1081 acataggtaa taaattgccc cgcctcatta aatccatttg tcaaacataa cttagctata
     1141 taactatgat aatgtttgag tatcatcttg attgaaattg gatcttcttc tagagcagat
     1201 tggataatgg atagaggaac taatggatac tgccgttttt tggggttgtc taccataagt
     1261 cataccccat ttttaattgt tcaactgcct tctttcgttg ataccagata ccacttgtac
     1321 tcatattgag gattttaccg atttcagtat cattaaaacc tgcaaaataa taaagtagga
     1381 taacctttct tcttatttcc gataaatgat caatggctgc cgataacttt tgatctgaaa
     1441 ttaatatttc cattccaaat aagaaataga catcttgttc atcaaaatga gtttgggagt
     1501 acttaccttc atttgaattc gtcaatggtt cctcattatc tgttatgtta atgtttcgtt
     1561 ttcgaataga cttagtttcg tttcgtaata cttttttaca gtaggcatca aattgatgtt
     1621 gtatagattt ctcattgtaa ttttgttgca tgataaatat gctccttttt tcccctttcc
     1681 actaatacaa caatcagaaa gcgccttttt ttcgaaaaaa ctagaaaaaa atatccttct
     1741 ttaaacagaa aatcttttta taaaaaaaag gagttggcaa ttaagcagcc actccttttt
     1801 tatattcctt ataaaaggta aattattttt tataactgaa atttggtatt gatgaccatc
     1861 gtttctttag atagtgagca atcattttag gctttctatc tctagtaaag attcctttct
     1921 tatttccttg cactcttaaa atcccttgac ttgtttcaaa atctgcaaaa ttccacactt
     1981 gttcaccaac aaaattgtca catttatcta ttacttcgtg atttgcttcg taataaccca
     2041 cttgatactc ttctgtaaac attgttggta ctgtatcatg aagtccagct actgtatctg
     2101 ctccatattc tgtaaacatt attggcgtat cgggacatct ttccatccat ccatctagct
     2161 cttttttcaa cagatatttt gcatgtttta ggtctccacc tgaaacatac caaccataat
     2221 agcgatttaa acataaaaca tcgacaatat ctcctacttt acaaatatct ggtgttgact
     2281 ttaagtaagt aacgattgtt acaggtctct tctgagggtc aagttcttta gtcaattcaa
     2341 ctaaaggttc aaaatattct ttagcgcctt cagaatccga atctggttca tttgcaatag
     2401 accacattac gacactcgga tggttcttgt cgcgttctat caattcctta atcgccattt
     2461 tgtgagcatt ttctgtccgt atttctttcc aagtatcatg tttttctacg ttgtcatcta
     2521 ataaagtagc catgaaattc aaatgtaatc ctactgcagg tacttcatca ataactacaa
     2581 taccttctcg atctgctaag cgcataattt cttctgaata aggataatgt gaagtcctaa
     2641 atgaattagc accgatccat ttcattaaat taaaatcttt gatatttact gcttcattca
     2701 aaccacgacc attaatatac gtgtcctcat gcttcccaaa tcccttaaag taaaaaggtt
     2761 tattattaat caaaaacttc ccattcgaaa cctgaatact acgaatgcca aattcttctg
     2821 tgtacgtatc aatcacttta ttttcgttaa ttaaactaac tttcatacta tataagtaag
     2881 catttagagg ctcccaaagt gttgggttct taagttgaat cactccttct ttccccttag
     2941 cttctgctat aagttgagct tcttcgtcat agattgctat tagaatttct ccggtccatt
     3001 ctgtatcgat attaaattct acatttgcat ctgaatcaat tatttttgta ttaatagtga
     3061 catcctttat atagtctttc ggagttgtat acaatttaac gggtctatta atgcctgcat
     3121 aattgaaaaa gtcaaaatta accaaatttt ctttttttat gtctccatct tcctgtatcg
     3181 tttctttcaa aatccctaca ggtaaagtgc tatcgtcaat aacattgttt acagccactg
     3241 taattctgtt acttccatct tttataaact gattaatttc tgattcaaat ggcgtaaacc
     3301 ctcctttgtg ttgattaatc aattttccat tcaaaaatac ttttgcttca tgtgttgcag
     3361 atccaaaacg aagaactaat ctttcttcta ataatttttt aggaagaatc acttcttttt
     3421 cataccacac ccatccaaca tgatttctaa tgttctctgt ttcaaataaa tcattataag
     3481 aagaaggaac tgaaattgga taactctctt tttcaaccat tggttctaaa gcaagttctt
     3541 cttgtaagtc atcatttgta tctaacttaa atctccaaag accgcttaaa tcaattactt
     3601 gcctcgtctc tgtcataatg ggatataaca tgtaaatcac tcctatataa t
//
LOCUS       DAYYCY010000196         3537 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000196 DAYYCY010000000
VERSION     DAYYCY010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3537
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.201"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            55..942
                     /gene="cdaA"
                     /locus_tag="ACHAZB_002546"
     CDS             55..942
                     /gene="cdaA"
                     /locus_tag="ACHAZB_002546"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HFM8615165.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            939..2108
                     /locus_tag="ACHAZB_002547"
     CDS             939..2108
                     /locus_tag="ACHAZB_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HFM8615166.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            2132..3487
                     /gene="glmM"
                     /locus_tag="ACHAZB_002548"
     CDS             2132..3487
                     /gene="glmM"
                     /locus_tag="ACHAZB_002548"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFM8615167.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
BASE COUNT         1140 a          648 c          843 g          906 t
ORIGIN      
        1 ttaaaacata tgatatacta gagaggaaat ctcaaaagag aagagaggct ttttatgtca
       61 tttcaaatcg gggaactttt taatttggac tattggcagc aaatgttttc cggtgacttg
      121 ttctctctga attttttggt gaacatactt gatattctgg ttgtctggta tcttgtatac
      181 aaactgatcc aactgctgcg aggcacaaaa gcgattcagc ttctgaaagg tgtagctatt
      241 tttattgtta tccgaatcgt agcggaaatc attggtctgc acaccttgtc ttggctgatg
      301 aaccaagtca ttacgtacgg ggtgatagct gctatcgtga tttttcagcc ggaaatccgg
      361 agaggactgg aacatcttgg acgcagttcg cttttcaaac aatcaaaaag cgaacagcag
      421 gaagatgaga agatgatctt gtcttttgac aaagccatcc aatatatgtc caagcgaaaa
      481 atcggtgcgt tgatcactat cgaacgtcat actggattag atgagtatat tgagacaggg
      541 attgctttag atgcagatat cactggggag ctgctgatca atatctttat tcctaataca
      601 ccgctgcatg acggtgcagt tattgtaaaa gaaggaaaga tcgcagtagc aagcgcctat
      661 cttcctctat cagaaagtat gttgatccct aaagaattcg ggactcgaca ccgggcagct
      721 gtcggtataa gcgaagtcag tgacgcgatt accatcgttg tctctgaaga aaccggggac
      781 gtcagcatca cgttagataa tgaactgatg gctggcttat cccagcaaga atatttggca
      841 atcttgcgta gagaactaat tgctgaagag aaaacaaaaa acaagaaaaa cgtgattcaa
      901 tctttcctag acggtatgac taagggaggg gatcgcaaat gatctcaaaa gagaaacgga
      961 caagcattct ttacagtttt attgcattgc tgttcagtat tgcgctgtac ttcaatgcaa
     1021 atggccagtc cgttccaaat acgctttctg gaaatgaagc gtataatcaa acagttacgg
     1081 gggtgccgat caagatttct tatgattcaa agcgctacta tatccatgga tatgaaaatg
     1141 cagtgactgt caaactttcc agtgcaaacc gagttcagtt gaatgcagag gcaaacgaag
     1201 atacacggat gttccgcgtc acagcagata tcactaagct gggcgaagga acacatgaag
     1261 ttccgctaaa aattcaaaat ttgagcagtg ctgtcacagc aaagatcgaa ccatcgacta
     1321 ttacagtaac ggttgagaaa aaggtaataa aagatttcga tgtggaaacg ttgattcctt
     1381 caacgttcac accatctgga tatgaacttg atgatacttc cgttagtcct aaaaaagtct
     1441 ctatcactac aggtgacaaa acattggcag aaatcaaacg agtagtagct gatgtggacg
     1501 cttcgatggt cacagatgat gggattaata gtgaggtccc aatacaggcg ctgaatgcag
     1561 caggggaagt attaagtatc gtctctgatc cagtgcaagt aacagtgaga gctgattcga
     1621 tcaagccaag caagtcggta cgactttatg ggatacagca aggaacacca gctgccgggg
     1681 tagaaagcta tgatttcagt ttttcaaaac tggaagcaga agtaagcggc tctagcgatt
     1741 tgcttgcatc gatcggtgac agtattgctg taccgatcaa tgtctcaggt atcaatcatc
     1801 gaacaactcg taaaattgaa ataccagtag aagaggggtt aagtatcaag cccgaatcag
     1861 ttagtgtgga gattacaccc gtgttgcaaa cgtcaagcag tagctctgct tcgacgagca
     1921 gtcggacaca agaaaataca agtacgagta gaagtatttc atctgctgat tcaggaacgg
     1981 aaccatcgac aacaaccatt tcttccagtt cggaatctgg gacaagcagc tcgaccacaa
     2041 gtacagaaga aacatcagaa acaagcgaat caacacaaat gagcagaaca aattcctcag
     2101 aaaattaagt taaaggagta attttacaac tatgggtaaa tattttggaa cagacggcgt
     2161 acgcggtgaa gccaacaagg aacttacgcc agaacttgca tttaagctgg gacggtgcgg
     2221 aggttatgtc ctaagccagc atgaagaaga aggaaaacgc ccacgcgtgt tagtagggcg
     2281 ggatacaaga atctcaggtc aaatgttaga agaagcgctg atttcaggac ttttatcggt
     2341 gggtatcgaa gtgttccagc taggtgtgat ctctacacca ggtgttgctt atctgacacg
     2401 tttgcaaaaa gcaagcgcag gggtcatgat ttctgcttct cataatccag cacaagataa
     2461 tggaatcaaa tttttcggtg cagatggttt taaactagta gatgaccaag agctagaaat
     2521 tgaagcatta ttagatgctg aagtggatga attgcctcgt ccatcagctg aaggattggg
     2581 aactgtagaa gaatttccag aaggcttatt gaaatattct caattccttg tacaaacgat
     2641 caacggtgac ctttccggtt tgaccatttg tgtcgatgca gcaaacggag caacttcaac
     2701 aagtgttaat cgtttgtttg ccgatcttga aacagatttt tatacaatgg gcacaaatcc
     2761 agacgggtta aatatcaatg acggcgtagg ctctacccat ccagaacgtt tagctgagat
     2821 ggtcgttgaa aaaggagcag atgctggact agctttcgat ggcgatggcg atcgtattat
     2881 tgcggtggat gaacttggaa acattgtcga cggtgataaa atcatgttta tctgtgctaa
     2941 atacttggca gaaaaaaatc gattgaaaaa agatacgatc gttacgactg tcatgagtaa
     3001 cttaggattc cacaaagcgg tggaaggaat cggtttgaaa gatgtcgtta cacaagttgg
     3061 tgatcgttac gttgtagaag aaatgcgcaa aaacgattat aactttggcg gcgagcagtc
     3121 tgggcacatg attttcttag attttaacac aactggagat gggatgcttt caggaattca
     3181 gttactgaat atcatgaaac agacaggtaa gaaattatca gagcttgcag cagaggtaac
     3241 aatctatccg caaaaattag tgaatatccg cgtaagtgat aaaaacggtg cgatggatgt
     3301 tccggcaatc aaaaaagtga ttgaagaggc agaagctgaa atgggatcag aaggaagaat
     3361 cctagttcgt ccttccggta cagaaccatt acttcgagtg atggcagaag cgcctactga
     3421 tgagaaagtt aattactatg tcgataaaat cgcggcagta gtcaaagaag aaatcggact
     3481 ggattaagag atattttcat aaaaaataaa agaacaagga ccagtgtcag atgtctg
//
LOCUS       DAYYCY010000197         3479 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000197 DAYYCY010000000
VERSION     DAYYCY010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3479
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.202"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..725
                     /locus_tag="ACHAZB_002549"
     CDS             <1..725
                     /locus_tag="ACHAZB_002549"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8615168.1"
                     /translation="REIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            775..1164
                     /locus_tag="ACHAZB_002550"
     CDS             775..1164
                     /locus_tag="ACHAZB_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM8615169.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            1151..>3479
                     /locus_tag="ACHAZB_002551"
     CDS             1151..>3479
                     /locus_tag="ACHAZB_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8615170.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKFQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDIQTDALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKSLKKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RCYSKLEKLMESKLARRFKVRRVTPSDLTYLIEHIYGEKGIPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPNRCLIEEKPRCLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLEN"
BASE COUNT         1133 a          573 c          742 g         1031 t
ORIGIN      
        1 cgcgcgaaat tttttctgat attacaggaa tagaggctac tattcaacca aatagggatt
       61 tcaataatta tgttaaagag ctcagagctt gtattaataa ggaggacact tttgacagtg
      121 agtttggatc tcttattgat aagctgacaa aaaattccat tactaatcaa gatatatata
      181 atgacatact tgatataaga gttagtgggg aaagtagtat tttttcagat aaaaaattaa
      241 tcaattctat aagaaaattg aatgatgcac aagttcttga attacaaacc cttaagccaa
      301 atgatttagt agttatccga cttgaactaa atggaaaaaa agttagttta tcgaatgcta
      361 gtgctggtca aaagacatct gcaattcttt ctatgatttt ggcgtatgga aattcaccac
      421 tcatattgga tcagcctgaa gatgatttgg atagtcaatt aattaataac ttaatcgtaa
      481 gaagtatcat tacaaaaaag gaaaacagac aaattttaat tgtaactcat aatgcaaata
      541 tcccagttaa tggtgattca gaatggttga ttagcatggg agattcaaaa gaaatatcgg
      601 ctgatcagtg cgggtcggta gatgaaccca atatcaagcc aagaatatgt actgtgatgg
      661 aaggtggcga ggatgctttt acaaatagag cgaaacgcta tggttttaaa caaattgtag
      721 aataacacta gagggtgaga catattatta tgtccgccct ttttaggagg aaaaatgaaa
      781 aaaataaaaa gctataccag tatctgggca gttgaaaaag tcatttacgc aatcaatgat
      841 tttcagttgc cttttcctgt gacttttaac caaatggctt ggttcgtttt atctttactg
      901 tttgtcatcg tatttgccca tgtgcctcca ctatctatga tagaggaggt ctttttaaaa
      961 tacttaggca taccggtggc tgtcacttgg tttatgtcgc aaaagacgtt tgatggcaag
     1021 aaaccactgg gatttttacg atcctttatc agctatcact tgcgatttaa ggtgactttt
     1081 gcaggaaaga aagtcaaaga acagaagaaa cgatgggatg aacccagtac tcttgtaagg
     1141 agtgtgaact atgtatccga ttaagtatat tgaaaataat ctcgtcttta accaagaagg
     1201 cgagtgtttt gcctactatg aattagtgcc ttacaattac tcctttttgt cacccgaaca
     1261 gaaatttcaa gtgcatgata actttcgcca actaattgcg caaaatcgtg aaggcaagat
     1321 ccatgcgtta caaattgcca cagaaagtag tattcgcgcc acacaagaac gatcaaaaaa
     1381 agaaattacc ggtagactca aagaagtagc gaaacaacga attgatatac agacagatgc
     1441 attagtatcc atgattggcg atagtcagat tgattatcgc ttttttattg gctttaaact
     1501 aattgcgaca gatgaagaag tcaatctaaa gagtctcaag aaatctttct tttctgggct
     1561 tcaagaattt gtctatggag tgaatcatca tttgatgggc gattttgttt ccttatccaa
     1621 tgaagaaatt cgctgctatt ctaagcttga aaaattaatg gaaagcaaat tggctcgaag
     1681 atttaaagtc aggcgagtca caccgagtga cctgacgtat ttgattgaac atatttatgg
     1741 cgaaaaaggg ataccgtttg aagaatacga atttcagcta ccaaagaaaa agctaaaatc
     1801 agaaacactg gtgaaacgtt atgacttatt acgtccaaat cgttgtttaa ttgaagaaaa
     1861 accaaggtgc ttacggatgg aacatgagaa tcatgagtcg tacgtagcgt atttaacaat
     1921 taatacaatt gtgggggaga tggaatttcc ttcctcagaa cttttctatt accagcaaca
     1981 acaatttaca ttcccaatcg acacaagtat gaatgtagag attgtgacga ataaaaaggc
     2041 tttagctacc gttcgtaata agaaaaaaga actgaaagat ttagacaatc atgcttacca
     2101 atctgacaat gaaacgaatt ccaatgtctt agatgctttg gattccgtgg atgaattgga
     2161 aaccaccttg gatcagtcaa aagaatctat gtacaaactg agttatgtgg ttcgagtgag
     2221 tgccgaatca gtggatgaac taaagcgacg ctgtgatgaa gtgctcgatt tttatgatga
     2281 taccaatgta aaactagtcc ggccctttgg cgatatgatg gggctacatg aagaattctt
     2341 accctcaagt aaacgatata tgaacgacta tattcagtat gttacgtctg attttttggc
     2401 ggggcttggt tttggggcta ctcagatgct aggagaatta gaagggattt actttggcta
     2461 caatgtggat acaggtcgta atgtttacct gaaaccggca ttggcttcac aaggagtcaa
     2521 gggttcagta accaatgcgc tggccgccgc tttcctgggt tctcttggtg gaggtaaatc
     2581 ctttagtaac aatctcttag tctactatgc tgttttgttc ggtggtcaag ctgtgattgt
     2641 cgatccaaaa ggggagcgag gagggtggaa agaaaccttg ccagaaattg cggatgaaat
     2701 cagtatcctc aacttgacga gtaagtcaga aaatcaaggt ctgcttgatc cttatgtgat
     2761 tatgaagaaa aagaaagact cggaaagttt agcaattgat attctgactt ttttaacagg
     2821 aatttcaagt cgagacgggg agaagtttcc tgtgttacgt aaagccattc gaacggtgac
     2881 tcaaagtgat caacgggggt tattacaagt tattaacgaa ttgcgaaaag acgggtcacc
     2941 aatcgcggaa aatatcgccg atcatatcga gtcctttacc gattatgatt ttgctcattt
     3001 gcttttctca gacggctcga ttacccaatc gattagttta gataaacagc taaatatcat
     3061 tcaagtggcg gatttggttt taccggatgc agaaacgact tttgaagaat ataccaccat
     3121 ggaattgctt agtgtttcga tgctaatcgt catttccact tttgccttgg actttatcca
     3181 tagtaaccga gaaatcttca aaattgtcga tttggatgag gcttggagtt ttcttcaagt
     3241 ggctcaagga aaaacgttat cgaataagct tgttcgtgct ggtcgggcca tgaatgctgg
     3301 ggtctatttc gtcactcaaa atagcgacga tttattagat gaacgtttaa aaaataatat
     3361 cgggttgaag tttgcttttc gcagtaccga tattcatgaa atcaagaaaa ccttggaatt
     3421 ctttggtttg gatcaggagg atcaaagcaa tcaaaaacgc ctgcgagatt tggaaaacg
//
LOCUS       DAYYCY010000198         3470 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.203, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000198 DAYYCY010000000
VERSION     DAYYCY010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3470
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.203"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..909
                     /locus_tag="ACHAZB_002552"
     CDS             <1..909
                     /locus_tag="ACHAZB_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8615171.1"
                     /translation="LLDDPYWVGKTSLGENIANKGTTFPIKSSAYEIYKGNTEEELII
                     GQTYTITLKGTKPASQTFVAYNYWNVNFGELKPVEGLTDVWSLTFTPTKLEPGLPKDL
                     RIFQLPKETAGACQIDWLKIEKGDTRTPNISQFKYFGEGLKDSNNPNDYSWDITPEYT
                     EKGLNDTVSLTEPQSIEGLKNFEDGIQSKGKSVLTSDDNKYEVVTLTVTNGNTGSAKL
                     YREGKTVTIYFFALNGKSSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSI
                     GADGAIKWRRNSSYGSAYSFVITYSI"
     gene            923..1072
                     /locus_tag="ACHAZB_002553"
     CDS             923..1072
                     /locus_tag="ACHAZB_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8615172.1"
                     /translation="MKVVYKSIKPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            1069..1515
                     /locus_tag="ACHAZB_002554"
     CDS             1069..1515
                     /locus_tag="ACHAZB_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8615173.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGVRSDGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYEALEHENGNLKQLNAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            1517..1654
                     /locus_tag="ACHAZB_002555"
     CDS             1517..1654
                     /locus_tag="ACHAZB_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFM8615174.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADKIINKDKSA
                     S"
     gene            1691..1984
                     /locus_tag="ACHAZB_002556"
     CDS             1691..1984
                     /locus_tag="ACHAZB_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8615175.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERRK"
     gene            1981..2205
                     /locus_tag="ACHAZB_002557"
     CDS             1981..2205
                     /locus_tag="ACHAZB_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFM8615176.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            2202..3221
                     /locus_tag="ACHAZB_002558"
     CDS             2202..3221
                     /locus_tag="ACHAZB_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFM8615177.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLSDGTNHSHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RPDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
BASE COUNT         1199 a          621 c          725 g          925 t
ORIGIN      
        1 ttactagatg acccttactg ggtaggtaaa acatcgttgg gtgagaatat tgcaaataaa
       61 ggtacgacgt tcccaattaa atctagcgcg tacgaaatat ataaaggtaa cacggaagaa
      121 gagcttataa taggtcaaac gtatactatc acgcttaaag gaacaaaacc cgcaagtcaa
      181 acctttgtag catataatta ttggaacgtt aattttggag aactaaaacc agttgaggga
      241 ttgacagacg tatggtctct aacattcaca ccaacgaaac ttgaaccggg tttgcctaaa
      301 gaccttcgca tttttcagtt gcctaaagaa acggccggcg catgccaaat tgactggctc
      361 aaaattgaaa aaggcgacac ccgaaccccg aatattagtc agtttaaata ctttggtgaa
      421 ggattgaaag atagcaacaa tccgaacgac tacagctggg acatcacgcc tgaatatact
      481 gaaaaaggct tgaatgatac ggttagtttg actgaaccgc aatccatcga aggattgaaa
      541 aatttcgaag atggtattca atcaaaagga aaatctgtat tgacatcaga cgacaacaaa
      601 tatgaagtcg taaccttaac agttacaaac gggaataccg gatcagcaaa gctttatcgt
      661 gaaggaaaaa ccgtcactat ttattttttt gcgttgaatg ggaaaagcag tggtggaaat
      721 gattcaacga tactaacaat tccagaaggc tatcggccac caattagttt tgagcaactg
      781 gttggctcga tagaccgttc tactttgaac agtgctcagt tatctattgg tgcagatgga
      841 gccattaaat ggcgaagaaa ctcaagttat ggatcagctt attcatttgt tatcacttat
      901 tcaatttaag ggaggaaatc taatgaaagt agtttacaaa tcaatcaagc cttacggatt
      961 cgagcaaatc attgtgaatg acgaagaaca tttaccggaa gaatgcacag aagtcgaacc
     1021 accgattcca aattggaaac cgaaattcaa ttactgggag ggaaataaat gaaaaacatt
     1081 tggaaatatg gccgtactgg cggagagtat gcaggaaaag tattggacga catgcttgta
     1141 tccgttcctt acacagatca gccaccgctc gaaggggttc gttcagatgg cgaaccgcta
     1201 acgattgctg atcagatgtt tgatcctaaa ttgaaccaat ggattatttt agcgaacgcg
     1261 ttagatcaca acgatttaaa caatctcaaa gcgatgtacg aggctcttga acatgaaaac
     1321 ggcaacctaa aacagctaaa tgccaaactc atgctaagcg atgtagcgat taaacaggaa
     1381 aatactgcat tgaaagaaaa agctgacagt ttagcacaaa tcaattcaaa aatgatgctt
     1441 gcttcgttac aaaatagcaa agacatttca gaaattaaag agcaactaaa tccagcttca
     1501 aaaggaggtg agtagtatgt ttagttttag cgatgtgaaa atgatgtatg attggggctg
     1561 ttttactgac gatcaagttc gactattcgt tccactatgc attacagacg aagaagcaga
     1621 taaaatcatt aataaagata agagcgcatc ttaattgatg cgtttttatt taaggtaaag
     1681 gagttgtcac atgattaatt taggggaatg gggaatgata gcaggatcaa taaccgctat
     1741 cgtttctttg attttattag taattagacc gattgctgca tctttctcga aaattactga
     1801 gactctttca aaagtaagcc acaatttaga tttgctgact aaagatttag aatcgagcaa
     1861 atcagatcga ttgatgattc atgaagaact aaagaaacac gatgaaagat tagatacaca
     1921 tacagaaaaa ttggtggaac acacgcaaca aatcaaaact ttgtttaggg agagaagaaa
     1981 atgaataata aaacgttcga agtactaaaa tggttcgcac tggtaattat tcccgcacta
     2041 gctactttcg tggggttagt tggtaaagcg ctcaattggc agtacacaga tatctgtgtt
     2101 gtcatcatta ctggttttgg cgcgttttta gggagtgtgt tgggtgtatc aaatcgaacc
     2161 tacaaaatgt tctcggctga aagcgaagaa ggaggaaaca aatgaaaaag aaaattacta
     2221 ttactgcgat gagcctgtta acggctcttt ttttattgcc aattaacgga tttgcctata
     2281 ctatcaacaa tgaatttaat ttgggcgcaa atgaaggtag ctcacaagta gcaaataatc
     2341 agtatatttt actgcatgaa acggctaatg aaacagcaac aggacgcaat gaagcgcagt
     2401 atatgcaacg ttcatggact agcgcttata ctgcttatat tgtgggagac ggcggaattg
     2461 tttaccaagt cggacaacct ggttatgtac agtacggtgc tggttcatat gctaatgcca
     2521 atagtcctgt gcagattgag ttacaacaca cacatgataa agcaactttt gaaaaaaact
     2581 ataaagcata cgttgaattg gctagagatt cagcaatgaa atatggtatt ccattaacat
     2641 tagacacgcc ttataaccaa ccaggaatca aatcacattt atgggtaact caaaacattt
     2701 ggggcgatca cactgatccg tatggatact tatcagaaat gggtgtaagt aaggaaaaac
     2761 tggcttacga tttagctcat ggatttacag acgaaaaccc aacgacttct gagaacaagc
     2821 ctgtcattga tccaacccga gcaggtgcag caaatccaac tttatcagat ggaacgaacc
     2881 attctcacat tgatcagttt ggagaaatcg aaaatgcgaa cttacacgtc gctggatggc
     2941 acatcgctaa ctataagtac gagtatattt tcattatgga ctacaatact gggaaagaat
     3001 tagctcgagt aagagctgat ggaatttata gaccggatgt aaatcaagct tataatactt
     3061 ctggaaatgt tggctatcat gtatctttta acatgcgtaa cttccctaat aagaaagtct
     3121 atgtcatgat gcgggcaacg aatgatccag agggaaacac taaaggcggt gcgcaagatt
     3181 tccatgacaa acgttggtat ttaaatattc ctaaacgata aaaatagctc ctcgttgagg
     3241 agcattacat aactatattg acaactataa aaatcattcg ataaaatagt gatgttatcg
     3301 catatcttca ctatcaccca taatagtcac actccaagct atgcgataac aggtttgttg
     3361 ccacacattc tactggttga ttgtttatgg ctttatgtgg caacaaccag tacccttagc
     3421 tcagttggtc agagcagacg gctcataacc gtccggtcgt aggttcgagt
//
LOCUS       DAYYCY010000199         3451 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000199 DAYYCY010000000
VERSION     DAYYCY010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.204"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2232)
                     /locus_tag="ACHAZB_002559"
     CDS             complement(<1..2232)
                     /locus_tag="ACHAZB_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFM8615178.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
     gene            complement(2904..3172)
                     /locus_tag="ACHAZB_002560"
                     /pseudo
     CDS             complement(2904..3172)
                     /locus_tag="ACHAZB_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          967 a          763 c          547 g         1174 t
ORIGIN      
        1 accggtttgt agcgtttctc cttctcggtt ctgtaactca aaaacagctc cttgaaggac
       61 ttcaccactg ccttcagcat gtttaattag ttcaacacca cccgtatcaa ggatgttttc
      121 aatggttaac gtcacttctt ctatggagta ttcatttata aaaacatcat gtaccgtcgc
      181 atctaaaaga tacccttccg gtgcttctgt ttctcggact tgatagctac ctacaggaag
      241 cggttcactt aaaaattctc cgttttctgc cgttatacct tcttgtatca cttgtgaatc
      301 gcttgataaa atttcaaatt ttgctcctgc cagcctttgg ccattctcat cagttttaat
      361 tactttaata cgcccatcga ttttccttgt tcttacagta ctcgtattca atcgattgtc
      421 actattcaca agcgttgcaa agtttaaaat ttgatcgccg accgcatacg cctctacatt
      481 gaccttaaaa gagagggtaa aacttgtcgg accaccaatc gtattattcg cactgaaacg
      541 aactattcca ttattgactt cggcatcaat gaaagaggga atatctgata gaaatgacac
      601 aaattccatg ccttcttcca aaacatcttc aataacaagc gtatttcttg ttccagaaaa
      661 catcgagaca tgcagagtat acgttagttc attttcattg agattgataa catttttgtc
      721 cacacttttt cttaaagaga ttgccggaag attactttca gggattctca agcggtagtc
      781 tcggctcatt acctgattat tgtaggtcat actcgctcga ttccgagaaa acccatcctc
      841 ttgtcttcct tcattgaaca agtcgacatc ggtcacttca agagcatatt caataaaata
      901 cgattgaccc tctgctagat taccaaagtc cacaacaagt gtattcgttt ccttattaaa
      961 agatactttg tccacaaatt cctcggaaac ataatgagtt ctaacgtttg cttcacctgg
     1021 attcgttact tctgcgatcc gaaccccacc tgaaataggt tctctatttc cagtggaact
     1081 ccatgtagtc ggattcggtg ccaaagttgt tccttgcggt agtacatcgg taaccactac
     1141 accgtctttt cgtgatcctt cataatttac ctgcaaaata aatttattac tgttggtcac
     1201 gtcttgcccg tcaatcagca gattcccaag atttccttgg ctgtcgattt ctctagagcc
     1261 atagtataat ttcgaaaaaa tgggtcttga tgaaggaata ggcgtaattg aaaattccgt
     1321 gtttgctgct cgctcagcat aagtaagact cacttgaaag tcattatttg gctcagtacc
     1381 gcctgattga aagaaagcaa cttcaaacgt gatactgaat gtatacgact gagcggcttc
     1441 atttaatcct tcataatcaa aggtaaatgg aaaaacaacg tctgtgcctt cttggtaaaa
     1501 tgccagtggc tttaaaggtg aatcatcagc gacctccact ctaatgaact caattaagcc
     1561 aatcgatgct ctaggaactc gtacgaccat ctccccatat tcttcaagaa tatcgccagc
     1621 gcctccttga atgtatccct ttaacgtttg tgtatgcctg ctcggcaaag tggggcgctc
     1681 agcagagatg ccgatgtcat aattcaagag ctcctcttgt ctttcaatct gagattcctg
     1741 cgttatagat ggctcagatg atctggcttc attaaaagat tcacttaagc tttcttctgg
     1801 tatattaaaa gattcacttg tgctttcttc tggtacatta aaagactcac ttgtgctttc
     1861 ttctggtaca ctgatttcta ttgagaccgt atttgattga atatcaccgc ttcttgcggt
     1921 caattcgtat ttacctgaat gctgggaaga ataagtaatc agacgttcgt aatcattgag
     1981 ttgcttcact tccccttcaa tcaaatcctc actcttatca atgaccaatg catttagcag
     2041 gtctggtgtt cctctgactc gcaactggaa ctgctcaccc aaagcagcct cctcttgatc
     2101 aacttcgatc gtcagcgaac gctctggctg ttctgctaaa gactcagcta gaaccgttat
     2161 gttcccaaat tgattgatca caataagcat aactagaaac atacttaaca gtttatgaaa
     2221 tttgttcctc actattactc ccccttataa ttttgactaa acaacaaggt acccgataat
     2281 tttctaaaaa gattccccaa ctcaaaatgg ctgcgttacc tccctcgtat tttcggttca
     2341 actcaagcgt tgcaatgtaa gctgtaaaca aagctaatga ttgccgattg tcttctgttg
     2401 attgattcga tagctttgta tgacttttag ttgtccgtgt ggcttaacca taattatgat
     2461 ttcacgattc ctcctgccag tgccttgcac aagctggagc gtaaccctaa tagttatttt
     2521 gttaatgtac ttatatactt ttatactttt ccattagaaa caatcactaa atcgttgata
     2581 ccaaggtcag actctagaat ttcatcagtg aatccctgta ttggttaacc ttcatcaatc
     2641 ccaaaaaaat atatcaatgc ttagcatcaa aatgaatata tagtttagaa tacttgggat
     2701 tcatcgataa ctgatcgctt atttggccat attatttggc acttggttca actaaacgca
     2761 tttaggatgt tccttcattc tcgtcatgga tgttctcaaa ccgtcattac taataaactg
     2821 tccaccgaat cgatggttca tttcttgcat tttccacgtc caattactct ttttgctctt
     2881 tatttgggcg tagtttcact ttcttaactt gtatcgtctt ccatcaactc cttaatcatc
     2941 ttcttagctt tattggctcg gtttccttgt aaaaggtatc tacaaatagt gacaatctgt
     3001 attaaatctt catcattttc tgtaatgtca ataatttcta tattctgtac catatattgc
     3061 aaagggtttc actaagttca taaccgaagc gaattaaaca acttttgtat agaataacta
     3121 ccttttctat ttttccattg gtaattctat ctattaattg gtttaatcct tttgttgtag
     3181 ttgattccac taccaagatg attctctcga attttaaagc ttgaagatgt attgctattt
     3241 ttgctaactg gcgtatttgc aactattttt cccttctatt cttccttcat tgattcccct
     3301 ctaaaattac tatccataaa aaattgcata aatgacaata gtatttatat tagtcatact
     3361 gctttttaac acagcaaatt ccagaaaaac attcgttaca tatttgtgaa tttttccaaa
     3421 aaataacact tttataaaac gacgaaaaat a
//
LOCUS       DAYYCY010000200         3437 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.205, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000200 DAYYCY010000000
VERSION     DAYYCY010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3437
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.205"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1245
                     /locus_tag="ACHAZB_002561"
     CDS             <1..1245
                     /locus_tag="ACHAZB_002561"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFM8615179.1"
                     /translation="VLKRGYYKEAVASIKQSFMLLLVCSLYLITMQDGNDYSRVSLFI
                     MGVVYGLLTYIIRILWKGLLHHRMKNGEDVSLVIVTSQKIAKNVVRNLKEKNYGMYKL
                     SGVIVVDRDMYGTTIGGVPVVANADNAAEYLLQNWVDEVFINVDESVPYPKELIARCS
                     EMGLAVHMNLTKVSDSTRGKQFIGRVGDYTVITSSINVMTMRQAFIKRTIDILAGIIG
                     CIATGVIFIFVAPVIYIKSPGPIFFAQERIGQNGKTFKMYKFRSMYMDAEERKAELMK
                     ENKMSNNLMFKMDFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQFYNVLMGKMSL
                     VGFRPCLKSEYEKYDFHHRARIAMKPGITGMWQVSGRSDITDFEEVVKLDAEYIRNWS
                     MGLDFRILFRTVKTVLHRNGSM"
     gene            1277..2413
                     /locus_tag="ACHAZB_002562"
     CDS             1277..2413
                     /locus_tag="ACHAZB_002562"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFM8615180.1"
                     /translation="MARKKLKICMLGHKRIPSREGGIEIVVEELATRMVALGHEVTCY
                     NRSGHHVSGKEFDQHKNKEYKGVKLKTIFTLNIKGIAAMSSSVFGGIRAAFGKYDVVH
                     FHAEGPCAMLWLPKLFGKRCVATIHGLDHQREKWNKLASTYIMLGEKCAVKFADEIIV
                     LSESVQNYFEDIYGRKTRFIPNGVKKIEIKSAGLITEKYGLTKDSYILFLGRLVPEKG
                     IRYLIEAFKDVQTDKKLIIAGGSSDTDEFANELKELAKGDERIIFTGFVQGQELEELY
                     SNAYIYTLPSDLEGMPLSLLEAMSYGNCCIVSNISECTEVVEDKAMIFKKSDVSDLKI
                     RLEEACNQSEMVKVLKNQATEFICSKYNWDKIVQETLNLYRGNI"
     gene            2473..>3437
                     /locus_tag="ACHAZB_002563"
     CDS             2473..>3437
                     /locus_tag="ACHAZB_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HFM8615181.1"
                     /translation="MNYYRNIDRTKIQFDFLTHRPYKSDYDEEIISLGGKVYYAPRLY
                     PQNYPKYFKWMEQFFKDHPEYEIVHSHIDAMSYLPLKAAKKAGIPIRIAHSHNTSIDR
                     DFKYLLKQYYRCKLTSVLTHEFSCGKVAGDFLFRNDKFTLIPNAVDAKKFYYDESIRN
                     DVRMKLGISSQTFVMGHIGRISYSKNHRFLIEIFKEFHAIKNNSVLLIIGTGDMEEEI
                     KNYAKKSGIDDDIKFLGNRNDIEKFYQAFDVLMLPSLFEGVPLVGIEAQFADLPCFFS
                     EKVPTEVAFSDKTNFISLNQSAKEWAREIANVDISHRDSERSILI"
BASE COUNT         1232 a          393 c          741 g         1071 t
ORIGIN      
        1 gttttaaaaa gaggatatta taaagaggct gtagcttcta taaagcaatc ttttatgctg
       61 ttacttgtgt gcagtttata ccttattaca atgcaggatg gaaatgatta ttccagagtt
      121 tctttattta taatgggggt ggtgtatgga ttattaacat atataattcg tatactttgg
      181 aaagggttgt tgcatcaccg catgaaaaac ggtgaagatg tatcgttagt tattgtaaca
      241 tctcaaaaga ttgcaaaaaa tgtagttcgt aatcttaaag agaagaatta tggcatgtat
      301 aaacttagtg gtgtgattgt agtagacaga gatatgtatg gaactaccat cggaggtgtc
      361 ccagtagttg caaatgctga taatgcagcg gaatatcttt tacagaattg ggtagatgaa
      421 gtattcatca atgttgacga aagtgttcca tatcccaaag aattaatcgc cagatgttcg
      481 gaaatgggac ttgctgtaca tatgaatctt acaaaagtgt ctgatagtac acgtggaaaa
      541 caatttattg gccgagttgg tgattatact gttattacat ctagtattaa tgttatgacg
      601 atgaggcaag catttataaa gagaacgatt gacattcttg ctggaattat tggatgtatt
      661 gcaacaggtg ttatttttat cttcgtagca ccagttattt acatcaaatc accagggccg
      721 attttctttg cacaagaacg tattggtcaa aatggtaaga catttaagat gtataagttc
      781 cgtagtatgt atatggatgc agaagaacgc aaagcagagt tgatgaaaga aaataagatg
      841 agtaataatc tcatgtttaa gatggatttt gacccaagaa tcattggcaa taaaatactt
      901 cctgatggaa caaagaagac aggaattgga caatttattc gatctacatc attggatgag
      961 tttccacaat tttataatgt cctaatggga aaaatgagcc tggtggggtt tagaccttgc
     1021 ttgaagtcag aatatgagaa gtatgatttt catcatcgtg cgagaattgc gatgaaacct
     1081 gggattactg gaatgtggca ggtgagtggt agaagtgata tcacagactt cgaagaggtt
     1141 gtaaaacttg atgcagaata tatcaggaat tggagtatgg gattagattt tagaattttg
     1201 tttaggacag tcaaaactgt gcttcataga aacggttcca tgtaagataa agatactata
     1261 gaaggagtaa aacgtgatgg caagaaaaaa attaaaaatc tgtatgctag gtcataaaag
     1321 aattccatca agagaaggtg gaattgagat agtggtagaa gaattggcaa cgcgaatggt
     1381 tgcacttggg catgaagtga cttgctataa tcgaagtggt catcatgtta gcggaaagga
     1441 atttgaccag cataaaaata aagaatataa aggtgtaaaa ttaaagacta tttttacatt
     1501 aaatataaaa ggaattgcag caatgagttc ttcagtattt ggtggaatta gagcagcatt
     1561 cggaaaatat gatgttgttc attttcatgc agaaggacca tgtgctatgt tgtggttgcc
     1621 aaaacttttt gggaaaagat gtgttgcaac aattcatggt ctagatcatc aaagagaaaa
     1681 atggaacaag cttgctagta catacatcat gttaggggaa aagtgtgctg taaaatttgc
     1741 agatgaaata attgtactta gtgaaagcgt ccaaaattat tttgaagata tatatggaag
     1801 aaaaacaagg tttataccaa atggcgttaa aaaaattgaa ataaaatcag caggattgat
     1861 tacagaaaag tatggattaa caaaagattc ttatattttg tttttggggc gattggttcc
     1921 cgaaaaagga attcggtatc taattgaggc atttaaagat gttcagacag ataaaaaact
     1981 tataatagcc ggaggaagct ctgatacaga tgaatttgca aatgaactaa aggaattagc
     2041 aaaaggtgat gagcgaatta tatttactgg atttgtacaa ggacaggaat tagaagaatt
     2101 atatagtaat gcgtatatct atacacttcc aagtgattta gaaggaatgc cattaagttt
     2161 gttagaagca atgagttatg ggaactgttg tatagtttca aatatctcag agtgtacgga
     2221 agtagtagaa gacaaggcta tgatttttaa gaaatcggat gtaagtgatt taaagataag
     2281 attagaagag gcatgcaatc aatcggaaat ggttaaagtt ttgaagaatc aagctactga
     2341 gtttatttgt agtaaatata attgggataa aatcgtacaa gaaacattaa atttgtatag
     2401 gggaaatata taatgattcg aatattacag tgtgtgaatg atatgcatcg tgcagggttg
     2461 gagacgatgc taatgaacta ttataggaat atagatcgaa ctaaaattca gtttgatttt
     2521 ttgacacatc gtccatataa aagtgattat gatgaggaaa taataagtct tggaggaaag
     2581 gtctattatg caccgagact ttatccacaa aattatccta aatattttaa atggatggaa
     2641 caatttttta aggatcatcc agaatatgag attgttcatt ctcatattga tgcgatgagt
     2701 tatttacctt taaaagctgc aaaaaaagct gggattccga tacggatagc acatagccat
     2761 aatacatcga tagatagaga ttttaaatat cttttaaagc aatattatcg atgtaagtta
     2821 acgtctgttt tgacacatga attttcatgt ggaaaagtag caggtgattt tttgtttcga
     2881 aatgataaat tcactttgat tccaaatgca gtggatgcta aaaaatttta ttatgatgaa
     2941 agtattagaa atgatgtacg aatgaaatta ggaatttcaa gtcagacgtt tgtaatggga
     3001 catattggaa gaatttcata ttctaaaaat catcgttttt tgattgaaat ttttaaagaa
     3061 tttcacgcta ttaagaataa ttccgtatta ttaattattg gaactggtga tatggaagaa
     3121 gaaataaaaa attatgcaaa gaaaagtggc atagatgatg atattaaatt tttaggaaat
     3181 agaaatgata tagaaaaatt ttatcaagca tttgacgtat taatgttgcc atcattattt
     3241 gaaggggttc cgttagtggg tattgaagct caatttgcag atttaccgtg tttcttttcg
     3301 gagaaagttc ctacagaagt tgctttcagt gataagacca atttcataag tttaaatcaa
     3361 tcggcaaaag aatgggcgag agagattgca aatgtagata tctctcatcg agattcagaa
     3421 cgttcgattt taataaa
//
LOCUS       DAYYCY010000201         3394 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000201 DAYYCY010000000
VERSION     DAYYCY010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3394
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.206"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(161..2158)
                     /gene="tkt"
                     /locus_tag="ACHAZB_002564"
     CDS             complement(161..2158)
                     /gene="tkt"
                     /locus_tag="ACHAZB_002564"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFM8615182.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2261..2512)
                     /locus_tag="ACHAZB_002565"
     CDS             complement(2261..2512)
                     /locus_tag="ACHAZB_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HFM8615183.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2659..3282
                     /gene="lexA"
                     /locus_tag="ACHAZB_002566"
     CDS             2659..3282
                     /gene="lexA"
                     /locus_tag="ACHAZB_002566"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFM8615184.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
BASE COUNT          925 a          746 c          626 g         1097 t
ORIGIN      
        1 tcaagaaaca caaactaatc cgaagaattc tttgttttgt catttaaatg taacatagcc
       61 gaaaacgaaa agtaatattg atttattctt ctaacacata aagaacagct gtctccaact
      121 tgcagacagc tgttcttatc agtcaaacaa ttgcttggat ctatgatagt tgattgtacg
      181 tattcacgac attttctact gtaaatccaa attcttctaa aattttagtg ccaggggcag
      241 atgcaccgaa atgatcgatt gtaatcgttg cgccctctgt tcctacgtag cgttcccatc
      301 caaatgatgc agcagcttcg atagccacac gtttcttcac tgatttagga agtacacttt
      361 ctttatattc agaagattgt ttttcaaaga ggtcaaagct tggcatagaa acaacagaaa
      421 catctttgcc tttttcagcc aattcttttt gagctttcac agccaaatca acttcagaac
      481 ctgttgcaat caaaatacct tctggtgttt ctccttgtga tggagaaaga acatacgcgc
      541 cttttttcac catttcatct gctacttctt ttgtagaagg caatactgga aggttttgac
      601 gactcaatac aaggatagta ggcgcatctg ttgtctccat cgctactttc caagcagcac
      661 gtgtttcatt tccgtctgcc ggacgaatca cttgaacacc aggcatgcag cgaacagatg
      721 ctagttgttc aatcggttcg tgcgttggtc cgtcttctcc gacagcaacc gagtcatgtg
      781 ttaagacgta aacaacagga gtatgttgga ttgctgctaa acgaactgct ggtcttagat
      841 aatcgacaaa gacaaagaat gttccaccgt agatacgagt tccgccatga agttggatac
      901 cattcatagc cgaagccatt gcaaattcgc ggacaccgaa ccagatgttg cgtccttcat
      961 aatgttcagg agtgaaatct ttatctgcag ccaccattgt attattggaa cctgacaagt
     1021 ccgctgaacc accccagaag cttggtatag ctttcgataa ttcttggatg acttctttac
     1081 ttgaaacacg gcttgcttga ctgcttccaa cttcataagt tggcaattct gcatcccagt
     1141 tttctggtag tttgccatca aaagcatctt caaattgttg tgctaattca ggataagctt
     1201 ttttataatt ggcaaacatt tcattccatg catcttcagc tttttgtcct tcttctatca
     1261 tcgtttgatg gaagcgggca gctacttctt ctggaacagt gaaatctgga tactcccaac
     1321 cataaacttc tttagccatc ttgatgccat cttcaccaag aggagcacca tgaacagcag
     1381 atgttccttc tttaggagca ccatagccga tgactgtttt tacttcgatc aatgttggtt
     1441 tgtctgtttc agcttttgct tcttcaatcg ctttagagat tgcttctagg tcatttccgt
     1501 ctttcactaa gatatgttgc cagccatatg cttcatagcg agcaccaacg ttttctgtga
     1561 aagcttttga agttggaccg tctagtgaaa tatcgtttga atcgtataaa acgatcaatt
     1621 tccctagttt catatgacct gccatcgaac ttgcttcttg agatacccct tccatcaagt
     1681 ctccgtcacc gcatattgca tacgtatagt gatccatcac attaaagttt tctttgttat
     1741 acgttgctgc aaggtgcgct tctgccattg ccataccgac agccattgca atcccttgtc
     1801 ctaatggccc tgtagtggct tctacaccat ctgtatgatg tacttcagga tgtcctggtg
     1861 ttttagaatc ccattgacgg aattgtttca aatcatcgat tgtcacttga tagccagcta
     1921 aatgaagcaa gctatacaac attgctgacc catgtcctgc cgaaagaacg aaacggtcgc
     1981 ggtcagccca gttttttgat gtcgttggat ttactttcag gtgttttgtc cataacgcat
     2041 atgccatagg tgctgcgccc atcggcaatc caggatgtcc tgagtttgct ttttggactg
     2101 cttcaatact taatgtacga attgtgttaa cgccaagttg gtcaattttg tcaaacaaaa
     2161 taacccctcc tattaattta ggtttttttt tacctttatt tcttacactt tcattctata
     2221 agatgtcaca cgaaaaagca atctttttca gtatttttta ctaaaagttg tcatttcggt
     2281 tgtgtaagcc ttttttcttt tggatttctt ttaatttttc cggggtcacg tcattgccct
     2341 caggatcgac gactttcatt ccttcgatat gatgacgcat accggaacga aaagcttcca
     2401 aatactcttt gcgcaagttg atttgttctt gttcttcggc ttttgagaga ccattttcct
     2461 tctttttacg tgcaagttca ttgatccgct ctattttttc aggtgacagc atgctcagcc
     2521 ctccttttac tctccttcat attatataaa aactgaaaaa aaaacaacta gataaactaa
     2581 ataaaatacg aacattcgtt tgatttagcg ctttttatat ggtactattt atttatcaag
     2641 tgaaagaagg tgtaccttgt ggcccgacaa actgaatcaa gacaaatcga agtcttacga
     2701 tatatccatg aacaagtaac ccaaaaagga tacccgccga ctgtccgtga aatcggggaa
     2761 gctgttcagc tttcttctac ttctactgtt cacggtcatc ttgcacggct tgaaaaaaaa
     2821 gggtttatcc aaagagatcc gacgaaacct cgtgcaatcg agttgacaaa acaaggttta
     2881 gaaaagattg ggattcgtcc aacaactatt ccagtacttg gcgtcgtaac agctggagaa
     2941 cccattttag cagtagagga tgcttcagac ttctttccaa tccctcctaa tctttcttcc
     3001 gaagaaggca gtctctttat gctgacaatt cgtggagata gtatgatcaa tgctgggata
     3061 ttggatggtg acaacgtgat cgttcggaag caagaaactg ctcaaaatgg cgatattgtc
     3121 attgcaatga ctgccgagga cgaagcgact tgcaaacgat tctataaaga aaaaaacttt
     3181 ttccgtctgc agcctgaaaa cgacttgctc gaaccaatca tcttagatca agtatctata
     3241 ttaggacgag tagtaggact ttatcgtagt catatctatt aattgctact aattaaaaga
     3301 aaacttttta aaaagagttt gagacgttgg tctcaaactc ttttttcgcc cgggttcttc
     3361 ttctttcaaa tttttatcac ttccctcttt acaa
//
LOCUS       DAYYCY010000202         3310 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000202 DAYYCY010000000
VERSION     DAYYCY010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3310
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.207"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(201..569)
                     /locus_tag="ACHAZB_002567"
     CDS             complement(201..569)
                     /locus_tag="ACHAZB_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HFM8615185.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            719..1201
                     /locus_tag="ACHAZB_002568"
     CDS             719..1201
                     /locus_tag="ACHAZB_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HFM8615186.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            1238..2248
                     /locus_tag="ACHAZB_002569"
     CDS             1238..2248
                     /locus_tag="ACHAZB_002569"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HFM8615187.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(2363..3076)
                     /locus_tag="ACHAZB_002570"
     CDS             complement(2363..3076)
                     /locus_tag="ACHAZB_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8615188.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
BASE COUNT         1128 a          523 c          648 g         1011 t
ORIGIN      
        1 aaataatttt actatgtaga aaattcaaag ccattcaatc agtgattttt ttcagcacaa
       61 gaaagactcc ttataaaaat agcagatact ttgtcaatta agtgtttaac cagggatgat
      121 aaaaaggata ggattgaaca aaacctgtat aaggtgatga atagtttcct cgccagtaaa
      181 aggtttattc gtataaagct ttatcgccaa aatttatcat gacctttttt atcttcaata
      241 ttaaatgaaa tcaattgttc aatcaattcc cagttgatta cacttttcca aggaactttg
      301 atgacgttat ccgtatatac gtagcctgct tcttggatat ttttttcaag ggcacgaatc
      361 gctgcttttt ccggtgcgat agaaaaatga tttttagcaa aactaaatgc aataataaag
      421 gtaccatggt cagtaaacat aggttggttc cattttacag ttgtgtctag ctggggatac
      481 gtttctgtta tcttagcaaa aaggtctttg atcttttgtt gatgttcttt ttctttgatt
      541 tgattgatga tcggctgaaa tttttccatt ctaaaacgcc tccattaagt ggtttgattt
      601 attttaccag aaaaacaata gttatgtata aaaaacgaac aaaacacaag taatgttgta
      661 accgctatat ttatatgata agatgtctca aataataaaa tagaaaagag acataaaaat
      721 gattgacttg gcaaagaaat tcttacacga aaatatatat tttgagttgt atcatcatcg
      781 agccatttat acaaatgaag atgctttggt aataaagaaa gaacaaggat ttacaggtac
      841 agagaccaaa agcttattct taaaagataa aaaagataat aactacgtat atctgacttt
      901 tactacgaaa caaacggatt tcaaagcatt agggaagatc gtcggtaaaa ggctttctat
      961 tgtttcatca gaacgcatgg aagaacaaac gggacaaaag cagggcgctg tttctccgtt
     1021 tggttatgaa aaacagattc cgattattat tgatgaagag ctcttatcaa atgaaaaact
     1081 tgtttttgca ccaggtagac cagatcaaac gatggtcatc caaacggttg acttaaacaa
     1141 aatcatccag ctacttgaga tgccgatatt tttgatgccg gcaaacgaaa aaaacaatta
     1201 accaagtaaa atagtgcata cttggaggtg aagcaacatg ataagttatc cattattaga
     1261 aagaaatgca acgattgggg ttactgcacc atcctcaggc gtatcagagg aacaccattc
     1321 gttactcgaa tcagctttaa agagaatgga agaaaaatct ttccaagtag ttactggaaa
     1381 gacgctttgg acgcaagata aggaaaaatc aggtccagca agaaaacgag cagatgaatt
     1441 caatcaaatg atgcaagatg aaaatatcca aatgatcttt ccgacttggg gaggagaatt
     1501 attgattgag attctcgatt ttgtcgattt tgatgctcta caaccaaaat ggatagtagg
     1561 ttattcagat attagtacgt tgctacttgc tgtcacatta aaaacaggga tagccactgc
     1621 acatggaact aaccttattg atttaagagg agagtacttg gatgaaacga cagcaatgtg
     1681 ggaaagtgta ctgaaaacaa gagaaggaga atcgattcta cagcaatctt ctgaaaagta
     1741 tcaaaaagtg tggccccatg agcattcatc atcttctgtt ttccatctca ctgaaccaac
     1801 aaattggcaa tcagtcagcg gagatgcagt aaggatgaag ggacgattat taggtggttg
     1861 tattgatacg atcaggcatt taatcggtac tccttatgga gatgtccaat cttttaaaag
     1921 aaagtataat gatggaaagc cgatcttgtg gtttctagaa aactgtgagc tatcgtcagt
     1981 tgatttaaga agatcacttg ttcagatgaa actggcaggc tggtttgatg gcagctcggg
     2041 gatcatgttc ggtagaagtg cggcagagga tttgttagat tataaaatga aggatgtata
     2101 cgaagatctg tcaaaggaat taaaactacc gatcttgtat gatattgatt gtggtcatgt
     2161 accgccacaa ctgacattga ttaatggtgc ctatgcagaa gtagcggtag aagatagaaa
     2221 agcgactgtt ttgcaaacgt ttatttaaaa aattttttcc tgccttggag tctatcaaag
     2281 accaataaat ctaaatagaa aagaaagtga aagaagctga caaaatgctg tcagcttctt
     2341 tttgtataac atattagaaa gtctaattat ctataactgt cacatcgatt aactgtttca
     2401 cgtcattggg atttcctgta ggaatagcta ctacagatgc attatcaatg atttcttgag
     2461 ttgtttctct aatgaacgct ggataagatg tttgatctgc aggcatttgc caatgtgaag
     2521 gaactggata actagtgatt gtcccgtcat ggttacttga atacactaaa actccctggt
     2581 aactgtattt cccagtgagc ataaccacat ctttcggata gactgcactc cctgcatcat
     2641 aaggggcgat aggtgttcct gcaggataat gaataacatg taattcgaaa tcacccgatg
     2701 tatctctaaa agtattacct ctaatagcta gccaaacacg tgcgtactca atttgtagat
     2761 cagaatatcc tgctaacgga tctattgact gagaagactg tgaggaggtt gtgtctattg
     2821 ttgaagaaga aatatttgtc tcaactgtac tactagatgt agaagaagta ttgggctctg
     2881 agactttact gcttgttgaa gatgtattcg tttcagtaga agctgatttc tgagtacttg
     2941 atttttcagc tgaactggtt tgtctgattg aacttttgtc tgtggtcgca gatggatcac
     3001 tattttcttt actaggagac gtacatccta caaggaataa agagaccaac aataaaataa
     3061 aacctaactt tttcatataa aatacctcat tattttttta ataccagtat agcataatcc
     3121 tgaatttttg aatccgtaaa accttgtcta tgaatataaa tagagtgtat cttctttgat
     3181 aaatgtcaga aaaagtgtga caaataatca tcagtaaaat tacttgtcac actattgata
     3241 aggagtattt aattgaagag aatgattagg agaaaggatc tccgtacttt ttatagtaat
     3301 catagaatcc
//
LOCUS       DAYYCY010000203         3272 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.208, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000203 DAYYCY010000000
VERSION     DAYYCY010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3272
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.208"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            588..1004
                     /locus_tag="ACHAZB_002571"
     CDS             588..1004
                     /locus_tag="ACHAZB_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HFM8615189.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            1018..1584
                     /locus_tag="ACHAZB_002572"
     CDS             1018..1584
                     /locus_tag="ACHAZB_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HFM8615190.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            1607..2593
                     /locus_tag="ACHAZB_002573"
     CDS             1607..2593
                     /locus_tag="ACHAZB_002573"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFM8615191.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(2748..>3272)
                     /gene="scm"
                     /locus_tag="ACHAZB_002574"
     CDS             complement(2748..>3272)
                     /gene="scm"
                     /locus_tag="ACHAZB_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFM8615192.1"
                     /translation="TSASSDSTDTTSTTSDSTDTSASSDSTDTSTTTSDSTDTSASSD
                     STDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSD
                     TRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVI
                     GLRITFMSYKKSKR"
BASE COUNT          980 a          612 c          698 g          982 t
ORIGIN      
        1 ttgttgcttt cgcattgatg aacggttccg gacgtgcgat tgggttggat cgctgggtaa
       61 ttccatggct acaacgtaca ctaggtaatt ggtggtacgg tagaccaagt tcccagtacg
      121 gcaagaaata aatagataaa gaacgttatt tgaatgcaag atttcaatag aaaggggctg
      181 agacaaaccg tcacagcctc ctttttatgt aaaattaaag gaataagatg cccgagataa
      241 gcctccatga gcaagacaca ggtgtcacaa cctctgttac aacctcttgt tataaacggt
      301 gttccagaag tagcttcttc gaaaaataag ccgaaatttc acaaaaactt gaaaagtaat
      361 tttcgtaaat tccttcttat ttcttgaagt tgcccacttc tgtcacaacc tcttgttata
      421 aacggtgttc cacctgctgc ttctcggtaa taagcccaaa gaattagaaa acatggaaag
      481 ctgttttcta ttcttcgttc ttattactca aagcaagaca caggtgtcac aacctcttgt
      541 ttttatgcta aaattgggtg agttaatctg gaaaaaggag aacaaaaatg agccttaaag
      601 aatttgctga aaaaagtctg ctcaaacctt gggatgtttt tattattttg cttttgactg
      661 ttctttcttt tttaccagtc atgatttttg gtcttcagca aaacaccgca acagcagaaa
      721 aagaagcggt actgcgtgtg gatggcacag aaatcaaaac attccctttg atggctggag
      781 aagagagcaa cacatatact tacaaagatg aacacggaga ttataacttg atcgagatag
      841 atggcgatcg agtacggata aaagaagcag attgcgatga ccagatctgc gtacgtcgag
      901 gctgggcaac gaaaaatggc gaaaccatcg tctgtttgcc acataaatta gtgatcgaag
      961 tccgttcaac agatggaggt gacgaagaca gtctgattta ttagaccttc acacaccatg
     1021 agtaaattac aaaaaatgat ttatatttcg ctacttgttg cacaaggagt gatcattggt
     1081 ttattggaaa atatgatccc ttatcctttt tcttttgcgc ctggtgcaaa actaggctta
     1141 gccaatctaa tcacgatcgt tgccattttt accatgaaaa aacaagacag cttcttacta
     1201 ctttggctac gactattcct gactactttg ctaggcggaa cgatttccac attcctttat
     1261 agtatgagtg gtgctttgct tagttatttt ggaatgttat tggtcaagca attaggacca
     1321 aaacgtgtca gtatcatcgg aattagtgcg gccggcggtt tcatgcataa tgtcggacag
     1381 cttttgatgg cttctttcat tgcacaatca tggacagtca tgttgtactt gccagtcctt
     1441 tcagttttag ggattttatc agggattgca atcgggatcg cagcgaatta tcttttacga
     1501 catgtgcaga cacttcaacg attccaaaaa gattatgagc agggaaccaa acaaaaatgg
     1561 agcccaatgt ttaccaaaaa ataatcgtca cacaaggagt ttttttatga agctacatcc
     1621 aatgtgggaa aactatcctg ctttaagtaa agaattgaca gaaacactaa aacggatgga
     1681 agaaactgtc cgcctgaaaa acaaacctgt cgaacaagcc atcaaagaaa cgatccatgc
     1741 cggcggaaag ctgttgcgtc ctgcctatca gctattgttc tcacaatttg gaccagagca
     1801 agatcgagac aaagccatcg ctttggcagc ttctatcgaa atgctccata cggccacact
     1861 gatccatgat gatatcgttg atgaagcgga tctgcgacgt ggtcagccta atatccgtag
     1921 tcagtttggt aatgtcgttt ctgtctatgc aggagactat ttatttgtct gctgttttaa
     1981 actattgtct gattactcca cttccttgaa aagtctccaa ttgaattctc gcagtatgga
     2041 aaaagtattg aatggcgaat tagggcagat ggatgatcgc tataattatg aactttctgt
     2101 aaaagaatat ttggaaaata tttcaggtaa gacagctgaa ctgttccaat taagttgttc
     2161 tgttggcgct tttgaaagtg gaacaagcga gcgcttcgca aaaaaagcag gagatatcgg
     2221 actttctatc gggatggcct ttcagattat cgatgatatt ttagattata caaaagagag
     2281 ccaggaaatc ggcaaacctg tactagaaga catgcgtcaa ggggtctatt ctttaccgtt
     2341 gatctactct ttgcaaaaaa acaaaccaaa gctactacct tatttagaga agaaagcggc
     2401 aatgacagaa gaagatgtcg acgccgtacg caaaatcgtt gaacatacag gaggcgtgga
     2461 agaagctagg aagttagcgg cctcttacac agaaaaggct ttgaaggaaa taaagaaatt
     2521 acctgccacc tctcttcgaa caaaagagaa tctcttttcc cttacccaac tgattttgga
     2581 tcgcaaagac tgaataggga aaggttcgat acgtaaataa atttctcaga aggaaatcta
     2641 cgtgtttttt gacaaattga attgtagttc ttgtatttat gataaaaagc aaaaataaag
     2701 agcaacgtcc gttagcgacg ttgctctttt ttatcttgaa tattttttta ccttttcgat
     2761 ttcttataac tcataaaagt gattcttaaa ccgattacaa gtaatataac gcctgcgagt
     2821 gttatccaat tattcgattg acttccagtt ttaggaagtg ctctttttga tttgtttcca
     2881 ttgtcattgc ttgttttatt agttaagtta aagttcgtcc tgttatccga acttgttttt
     2941 gagaaggaga cacttgcatg acgtgtatcg cttgtggtag acgacgtgtc tgtactatcg
     3001 cttgaagttg atgaatctga gctatcactt gtggtagagg atgtatctgt actgtcactt
     3061 gaagctgacg tgtccgtgct atcacttgta gtggtagatg aatctgagct atcacttgtg
     3121 gtagaggtag tatctgtact gtcacttgaa gctgacgtgt ccgtgctatc acttgttgtg
     3181 gtagatgtat ccgtactgtc gcttgaagct gacgtgtccg tgctatcact tgtggtagag
     3241 gtagtatctg tactgtcact tgaagctgat gt
//
LOCUS       DAYYCY010000204         3228 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.209, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000204 DAYYCY010000000
VERSION     DAYYCY010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3228
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.209"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1079)
                     /locus_tag="ACHAZB_002575"
     CDS             complement(<1..1079)
                     /locus_tag="ACHAZB_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine peptidase family C39 domain-containing
                     protein"
                     /protein_id="HFM8615193.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSG"
     gene            1337..1573
                     /locus_tag="ACHAZB_002576"
     CDS             1337..1573
                     /locus_tag="ACHAZB_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HFM8615194.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            1592..1807
                     /locus_tag="ACHAZB_002577"
     CDS             1592..1807
                     /locus_tag="ACHAZB_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HFM8615195.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
     gene            complement(2510..3226)
                     /locus_tag="ACHAZB_002578"
     CDS             complement(2510..3226)
                     /locus_tag="ACHAZB_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8615196.1"
                     /translation="MQLNQLERIIDNFILFHDKVFKIVLKTQSPLEVKKYLKQFRPKQ
                     GIYFLDIDLNHEVNGIELAEVIRKYDVQAKIIFTTTHDEMLPVTIKRRVETLGFVTKD
                     QTLDEYRNEIVELLLLAQERIDATKQVTNQAFVFSIGSQTFTFDINDIYFVEASKLPH
                     RLSLCTKDGQYEFYGRISELEKKYPMLTRISRACLVNIFNVKEIDFKKRSLYFDSELA
                     RNFTLGKAQKIKEKLKESTV"
BASE COUNT         1100 a          536 c          513 g         1079 t
ORIGIN      
        1 tcaccgctat atgctttaat tgtttctatt cctttaagac tttctatgat actagaattt
       61 acagctgcac cggctgccat ttcttccctg ttcgattttt catagctttt cacgaaagcg
      121 aatattgcaa ctacataaaa aggcaaagag catagagtga ttaaaaacaa cgtactattc
      181 tgaatagcta acgtaacccc aacaagcaac accataccaa tatctaaaaa tattgataac
      241 gtagcacttg ctaaagcatc aataatctta ctggcatcaa gaaaacgaga aataatttcc
      301 cctgattttc tcgtagcaaa aaaattcatt ggtaaagtta acacatgttt gaaatactga
      361 agcataattg ccatactcat tcgctgtcct aaaataataa gaagataact acgactatat
      421 tcaaacaata cccgaaataa atagacagcg atcaagccaa tggaaattat atttaaagtt
      481 gaacgtgctt gattagggat gaaaaaatca agaatacctt gaaaataata tgagcttcct
      541 atgccaaaaa acgtaatgaa aatcgatgct aaaacgatgt ggaatactaa tcctttttgt
      601 ttccaaatta taggcaaaaa agaacttaat ccagctactt tttctttaat aggaatataa
      661 gtttctctag gcataggtaa taaaagaata cctgtccatt ctttttgaaa cgcttcaatc
      721 gtttttctat tttttccatt agcgggatct gcaatccata gtacgtttcc cttcgtttta
      781 taaattacaa cgtagtgcat atagctgtta tcaaccaata tatgtgcaat taatggtaag
      841 ataagatctc tttctttcca gatctcacta tctgcttgaa tagccataca atcaaaagac
      901 aatttttcaa atgtcttttt caatccaaat gctgaagtac cctctaaatc agtcccagat
      961 aggtcccgta atttatgaat cgggatatca gttttaaaat attttaaaat catcgaaagg
     1021 caagcaacac cacaatcttt ttcatcttgt tgctgcacaa aaggaaatga ttttatcact
     1081 tatctaacac aactccttct aaaaaatttt tatagaaaaa ttctcaatat agctttaata
     1141 cagtgaaaat attgtctcat aattccacaa aaactttgaa aacagttctt gaacactaac
     1201 tttttcattc ctgaatcaaa tttattttgg aaattttact attcaagaat aaacttgata
     1261 agtttcagga agatttttac tgttaatcaa atacgtatgt tacagtagca ccgtaaaaaa
     1321 tattatttaa agaagcgtgt ttaaacatgc aaaatgtaaa ggaactaagt attgtggaaa
     1381 tgcacaaact gttggtggag gcaataatgc ttggggaaaa ttggggcaag ttgtaggtgg
     1441 acttaccaca ggggcagtag gtggtgcagg tttaggaaca gcaatttgcg gaccagcttg
     1501 cggtgttgtt ggagggttat atggagctgt cgctggaggt gctgcagctg gctgggatgc
     1561 tcgaaaaaaa taaaaaagga gaataagtag tatgcatatt aaaaacacaa aaacaacatt
     1621 tatattatct tcagaagagt tgaaaaatat tcaaggtggg agtgccgtgg gagttttagg
     1681 cactactttt tctggggcaa cagcaggagt aaaactttgt tcagccggag gaccttatgc
     1741 aatagctgct tgtggtgtag gtggagcttt attaggtgct ggattcagca tgtggaccgg
     1801 tgcgtaaaca attaaaaaat atcaaacctc aagattggtt tgtgtcgatt acgaacatag
     1861 gtatgttctt tatcttttca aaagtttgga atggacagaa ctttttacta aagcttcttg
     1921 tgctagtttt tataattata ttaaattttt atcttttatt aaagtcagat aaaaattctt
     1981 tggaaatgta atggtagaga tatttttaca aaaggacttg cagcgctgta gttggtgctg
     2041 caggaggacc tatttcggcc gttacaggag gtattggtaa tgtacttgtc ggatgtgcat
     2101 taaagcctgt tcctgcaagt taaatttata aaaacaggaa agtaattatc tcaaatccta
     2161 tcaagtacaa tcataggatt gcacttgaca ggacaaattt tttaactaaa aaatgaaaaa
     2221 cactttctaa aaattagtag aatttgccaa atctttcgca tcttccacca tacatttaat
     2281 tttaattccc ctttatggta cttaataaaa acaagaaaag aactattcat ttggcccttt
     2341 tttggttcca gaccatagaa atattcctcc agtaactaat attgctgtta tgcacgtaac
     2401 taataacagc tataaaaatt ggtacaactt taataggatt cagaaaaatt tgcactagtg
     2461 aaggaatcta ttttattaga aaaatgcaat ctccctaaag aaaaaaccac taaacagtgc
     2521 tttctttcaa cttctcctta attttttgtg ctttccctaa agtaaagttt ctggccaact
     2581 ctgaatcaaa atatagtgaa cgttttttaa aatctatttc tttaacgtta aagatattta
     2641 ctaaacacgc tcgactaatt cttgttaaca taggatattt cttttcaagc tcactaattc
     2701 ttccataaaa ttcgtactgc ccatctttag tacacaatga taatctatgt ggtaattttg
     2761 aagcttctac aaaataaatg tcatttatat caaaagtaaa tgtttgagaa cctatggaaa
     2821 aaacaaatgc ttgattcgta acctgttttg ttgcatctat cctttcttgc gctaataaca
     2881 ataactcaac aatctcgttt cgatactcat ctagtgtttg atcttttgtt acaaatccta
     2941 acgtttcaac tcttcttttt attgttacgg gtaacatctc atcatgagta gttgtaaaaa
     3001 tgatttttgc ttgaacatca tattttctga ttacttctgc taattctata ccgttaactt
     3061 catgatttaa atcaatatct aagaaatata ttccttgctt tggtcggaat tgtttgaggt
     3121 attttttaac ttctaacgga ctttgtgttt tcaatactat cttaaatact ttatcgtgaa
     3181 aaagaatgaa attatcaata attcgttcta actgatttaa ttgtataa
//
LOCUS       DAYYCY010000205         3226 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000205 DAYYCY010000000
VERSION     DAYYCY010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3226
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.210"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(653..2107)
                     /locus_tag="ACHAZB_002579"
     CDS             complement(653..2107)
                     /locus_tag="ACHAZB_002579"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8615197.1"
                     /translation="MFDESLHLFPEYPDTVGSQAIPEKKQAVCQILLKEEVQLKQELT
                     AVILMDRICKEVLAGSDGTLAYGVVPLFDEVVRLLGNTQQIGAICGEFLLLPKMLVTV
                     ELAELATGMNEHPYMSGYFYLTVDDKNRQALMQAVTRLLTEKKLNILVGTVSLLGEGW
                     DCPAINLLILANQASTFVQTQQLRGRGLRLGAQNKVTNIWHLAIGLPGVPLSEQPDLK
                     RMLRRLGNISGLTFESSPKIESGGDRFALPLEYTTTTMETYTQSMFYFSCERSRLKEQ
                     WSTALADGWHLSMPIVVRKVDQVKQKQETSLPIVVKNIEPLSFWSAIFKGNLLEYFQI
                     RRRQKLWEKECARKKSLAICVQTTLQRLGEVSDRKVIVAADQQEFRCYLENAGYREGQ
                     LFTEAMRELLAPLENPRYLIEGKKIVLTVPERFGRNKQIANLFLNELNQSSLPKCQLV
                     YTRNPEGRQKLVTYRMRQVFQTRDEVIIQKNIWT"
     gene            complement(2108..2407)
                     /locus_tag="ACHAZB_002580"
     CDS             complement(2108..2407)
                     /locus_tag="ACHAZB_002580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8615198.1"
                     /translation="MESQETVDYLLSLEFVQHPLEHSKEIYENFELFTSALIYLNHHN
                     YELIPEHWQLLGSSKQQGKKASVPSLGKKELITIYQQMFEDQPELPIFYQLKKAG"
     gene            complement(2710..3081)
                     /locus_tag="ACHAZB_002581"
     CDS             complement(2710..3081)
                     /locus_tag="ACHAZB_002581"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFM8615199.1"
                     /translation="MWLSDEKIQWRSYQKRILTNFATYLNNNSVHLVAPPGAGKTFLG
                     IEMIRRLDCKTLILVPSLILKKQWLTTIEEYFLVNPADSCYLSGAIEHPSHITVETYQ
                     TIYNRLKEEPTYGDQLELLVF"
BASE COUNT          982 a          700 c          532 g         1012 t
ORIGIN      
        1 tgcagactga taatagcaaa agaaaactta taatcaaata atatcaacat ttgatcacat
       61 aatttgagat tgaatgctga tttacctatg ttttacgtaa tggtctttag ttatttttgc
      121 tcataaaatg cataaaacac gcgtttttat gtgtgcttga aatttcttgg ctctataatt
      181 tctaagactg ataaaaatat tctatcggtt ctttttgcaa ataaaaaaac atatagaagc
      241 tccattagat tgaatcacca caacacaatc aaaggagcat tccgtatgct tgattttcat
      301 tctataaaac tacttttagg aatcaaagaa aatcacaacg gttcaaacta aaagcaagaa
      361 tatccgaagt accattgttt catcgatcat gagaaaagca ttcacctctg tctgcagatg
      421 ttcttatatc gctctgtcag tcctattaat aaagtactgt tttgttttca aaaaaactat
      481 tacgagtact ttttaagaga atgccttgca aatattcatc acacaccgtt atataagtca
      541 aatagtttct ttcagtcgtc aagaaaagat tctcctgtcc ttggcattat cagttaagta
      601 gcaacttaat tctcggactt gttctactct agcaaaattt attttcatca acttacgtcc
      661 aaatgttttt ttgaataatt acttcatcgc gggtctggaa gacttgcctc attcgataag
      721 tcactagctt ttggcgtcct tcaggatttc tagtatacac caactgacat ttgggcagcg
      781 acgattgatt taattcgttt agaaaaaggt tagcgatttg cttatttcgg ccaaagcgtt
      841 ctggtaccgt cagaactatc tttttccctt cgattaaata ccgcggattc tccagcggtg
      901 ctaaaagctc cctcatagct tcagtaaaaa gctgtccttc tcgatagcca gcattctcaa
      961 gatagcatct aaattcctgc tgatcagccg ctacgattac ttttctatca ctaacttcac
     1021 caaggcgctg caacgtcgtt tgaacgcata tggccaagct ttttttgcgg gcacattcct
     1081 tttcccatag cttttgtcgc ctacgaattt ggaaatattc cagcaagtta cctttaaata
     1141 tagctgacca gaagcttaga ggctctatat tttttactac tatcggcaat gaagtttctt
     1201 gtttttgctt tacctgatcg actttcctaa cgacgattgg catagatagg tgccagccat
     1261 cagctaaagc agtactccat tgttctttca accgacttcg ttcacaagaa aaatagaaca
     1321 tcgactgagt ataagtttcc atcgtggtgg tcgtatattc cagtggtaaa gcaaagcgat
     1381 cgccaccact ctcaattttt ggtgaagatt caaaggtcag acctgaaata ttgcccaaac
     1441 gccgtagcat tcttttcaaa tcaggctgtt cagacagagg aactcccggt aatccaatcg
     1501 ctaaatgcca aatgttcgtt actttatttt gtgcacctag gcgtaagccc cgccctcgta
     1561 gttgttgtgt ttgcacaaaa gtggaagctt gattagccaa gatcaacaga ttaattgcag
     1621 gacaatccca gccttctcct agcaaactaa cggttcctac caaaatattc aacttctttt
     1681 cggttaatag gcgagtaact gcttgcatca atgcttggcg gtttttgtca tccaccgtca
     1741 aataaaaata acccgacata taaggatgtt cattcatacc cgtcgctagt tctgccagct
     1801 caacagttac tagcattttc ggtaacagta agaactctcc acagatggca ccgatttgct
     1861 gagtgttacc taatagacga acaacttcat caaacaaagg aaccacgcca taagctaagg
     1921 taccatcact gcctgctaat acctccttgc aaatacgatc catcaaaatg accgctgtca
     1981 gttcttgctt cagctgaact tcttctttta acaaaatttg acaaactgct tgctttttct
     2041 caggaatcgc ctgactacca acagtgtctg gatactctgg aaacaaatgc agcgactcat
     2101 caaacaatca tccagctttt ttcaattgat aaaaaatagg caattccggt tgatcttcaa
     2161 acatctgctg ataaatagtg atcagctctt ttttacctaa agatggaact gaagcttttt
     2221 taccttgctg cttagaactg cctaatagct gccaatgttc gggtatcaat tcatagttat
     2281 gatgattcaa gtaaattaat gcactagtga acagctcaaa attttcataa atttccttcg
     2341 aatgctcaag cggatgctga acaaattcta aagaaagcaa ataatccacc gtctcttggc
     2401 tttccattaa cgcttccacc agctcatttt gtttctctaa aaaggtaaaa taatgctgag
     2461 tagtttcctg attagccgga atcaaatata gaaaatcctg atgaggacat agaacctcct
     2521 cctttaccaa ctcgggaatg ctaatttctt cgtcaatcag gccattttaa ctcaatatat
     2581 tgcttccact ccgtctgaga gctatcaatc ggaggcgtgg ctgtcaagga aatcgtcaac
     2641 agattcggtt gttttttctt caagtcaatc aacaattcac cccagctttt ttttaaatga
     2701 tgagcttcat caaaaaacta acagctccag ctgatcacca taagttggct cctccttcag
     2761 tcggttgtaa atggtctgat acgtttccac tgtaatatga ctaggatgtt caatcgcccc
     2821 agaaagatag caactatctg ctggatttac taagaaatat tcctcgattg ttgttaacca
     2881 ctgtttcttc aatattaggg aaggaaccaa aattaaggtt ttacaatcca aacgtcggat
     2941 catctcaatt cctaaaaagg tcttgccagc ccctggtgga gccactagat ggactgaatt
     3001 gttatttaaa taagtagcaa agttagtcaa tatacgcttc tgatagctac gccactgaat
     3061 tttttcatca gatagccaca tcccaggtcc ccctctcacg tattttctct atcataatct
     3121 aaatgaatac agcaaaaaaa agatttcttc aagaatatgt gtttttatag taatatttta
     3181 cttactctaa actcaaatga aaaggtaaaa aaactggtag tacaat
//
LOCUS       DAYYCY010000206         3221 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.211, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000206 DAYYCY010000000
VERSION     DAYYCY010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.211"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            301..585
                     /locus_tag="ACHAZB_002582"
     CDS             301..585
                     /locus_tag="ACHAZB_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8615200.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            588..1004
                     /locus_tag="ACHAZB_002583"
     CDS             588..1004
                     /locus_tag="ACHAZB_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8615201.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1141..1860)
                     /locus_tag="ACHAZB_002584"
     CDS             complement(1141..1860)
                     /locus_tag="ACHAZB_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFM8615202.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLE
                     FTFSSLTSDLPKISLRNWLEE"
     gene            2544..2630
                     /locus_tag="ACHAZB_002585"
     CDS             2544..2630
                     /locus_tag="ACHAZB_002585"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFM8615203.1"
                     /translation="MFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(2932..>3221)
                     /locus_tag="ACHAZB_002586"
     CDS             complement(2932..>3221)
                     /locus_tag="ACHAZB_002586"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8615204.1"
                     /translation="EYRRQRSDFISRKIVRLEKEKMEKFPYEYFQNLEKLEQAIQAIK
                     FDLTDEKLLEYKNMTPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
BASE COUNT         1074 a          599 c          503 g         1045 t
ORIGIN      
        1 aaaaaaaatg tcgtttgaaa aactaaataa atttttgatc gtgcattttt aaatttggtt
       61 atcgattaca cgtgcagcac tcccccccga attttaaaga gcataaggaa acgtgtaccc
      121 actttctaaa aaaagtgggt agataggctg tgcctatcac gtttctcaga aaaaaatttg
      181 atatcacttt ttcgccatcg tgggtagctt atgtatagca ttttggcaaa aaacagctat
      241 acaaatatag tatttgtatg gtatacataa attatacata attgcaaaag gagatttttt
      301 atggacattt tagttcggaa tattgatcca aagtttgttg caacaataga caaacgttgc
      361 caagaactca cgaataaaac aggagaaaaa tggagtcgaa ataaatatct aaaagtttta
      421 atcgaaagtg attttgaccg tcaacttacg acttacaaaa aagaccaatt cgatttattg
      481 ctcgataaat ttattgctat ccaaacagaa aatactcaaa tattaagcga gtatgtctac
      541 acaaataatc aaatcattga aatgcttatc ggaggaatga tataaccatg agaaaaactt
      601 ctgtccaaaa acatgttact ttgtacccta gtcatgttga cgttttagat tccatcatcg
      661 aaaaaaatcc agatatcaat aactactcgg aagccattcg ctttttatgt atgagttatg
      721 aacccgatac caacacgacc actaccagca atcaggcaaa actaaatgct atgggaaaag
      781 aaatttccat catgactgaa atcatcgcag aatttgctga tttatatcta aaagatacag
      841 gcgttcttat tggcacaaat tctaaagtat atcaagaagt gaaagaaaaa atcgaagcta
      901 aactaaatcc atcaaatcga aatcgaccaa agaatcatca aaaagaaacc agtcaaacag
      961 tcacttctag aaatttcgac ccaacaaatt ttctaaaacg ataaaaaaca gataggagtt
     1021 attcctatct gttttttatt tagaagcccc atacaatatg ctagaatgta ctagcaatct
     1081 tagtcggttg taagggactt gtctacttgc ttaatgtaag gggggcgagt tcttttttat
     1141 ttattcttct aaccagttac gcagagaaat ctttggcaaa tctgacgtta ggcttgaaaa
     1201 agtaaattct aatttggcga ttttattgcc cttttttgct tttatttttg tgactttaag
     1261 atcattgaag tattttttta gttctcgtaa tgcaggattt aatacttttt gatcaatgtt
     1321 acccatttga tagtattctg ggatatccat taagctccta aactcttcaa tacgaactac
     1381 ataataacca gtagaacggt actgcataag cagtcggaat agagtttttg catacgtaga
     1441 acgcaacgac gtgaaagcag ataattcaaa tctactaaat tcagctgtta aaccattaat
     1501 aatatattct aggtccggat taacactgac ttcaacaaac ttttttgttt tattgatttc
     1561 aaaaccagta aacaaaacaa agtagcgaat agaatcctca tcttcagtac ggtaagtaag
     1621 attaagcata tctttataga gcttttctaa atcaccgaca aaggcttttg tagcagtcat
     1681 cttataatca cttaactctt ttaattcctc gaaagaaaat ctaacttttt tcaaaccacg
     1741 atctttcata cgagcgcata atgcaaaaaa taaatccatc tgcttagcat tgaaattttt
     1801 cagcggtact aaattcaatt cattttgata tttgactgtt aattccgttt tttcatccat
     1861 tcctttcttc cccccttaca aataaataat aacctttaac aaccaactcg taaataggtt
     1921 ggttgttatt atacaaaagg atattagtac tcagataaaa agttgttagt actcagataa
     1981 aaagttgtta gtactcagat aaaaagttgt tagtactcag ataaaaagtt gttagtactc
     2041 agataaaaag ttgttagtac ccttctgtat ccgttgggag agtaaggctc aactgccccc
     2101 taaaaagggt tctaaaaaat ctttaaaaaa taaataaaat aaacgcgcgc gcgcgagcaa
     2161 aatttcccgc caaaaaatca cccacttttt aaaataaaca ggttcgcgca tgatattaaa
     2221 ctgccaatct agctccctat ggtcgctgta agaaggagtg atcgctacgc tctcacattt
     2281 tacttgcagc agtcgccttt aggctccttc gccaaaaata attcattagg gactcctttg
     2341 tggcgtcctg agagctcgtc taagccgttc taagacaaga ttcgataaaa actatgtcct
     2401 tcgttctaaa gcgccttaca ggcggtcagg gcaagcccta acaaccccaa ggcaataaaa
     2461 aacttaggct tatttttgct tttttattgt ataattgatt gtcgcctatc cgacagtctt
     2521 gcgactggaa ggaggtgtgt ttcgtgttca ctaactttat ttgctccctt cttgttgggc
     2581 ttttaagcgc tttgttcagt tactggttga ataaccgaaa caagaagtaa aacttaatta
     2641 aggcgactca gcccacaaaa aaagccccag agaatccgac ctctctgggg ctttcgtgtg
     2701 tttcgtgttc acttatttgc aatcaaatta taacagaaat aaatatttat ttcaaaaata
     2761 tcctacaaaa gtctaaaatc cgtaattatt tcttatatgc aacatttaca aataaagtcg
     2821 ctctgagagc tcgtattttt cgatttaaga cgttttattt agtttttggt gtatttcatt
     2881 ggtccaagct aaaaaactct tagaagcgat tttagagcca tttaaaccca cttaattata
     2941 ttctccatga aattcgctcc atgcgtcaaa agctgcgtct gaaattccgc cgtcttcgtt
     3001 cactagttgc attaattttt ccaacgatcg ttttctttcg ggtgtcatat ttttatattc
     3061 caacaatttt tcgtctgtta aatcgaactt gattgcttga attgcttgct ctaatttttc
     3121 taagttttga aaatattcgt aaggaaattt ttccattttt tctttctcca gtctcacaat
     3181 ttttctagaa ataaaatcac ttctttgccg tctatactct c
//
LOCUS       DAYYCY010000207         3201 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.212, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000207 DAYYCY010000000
VERSION     DAYYCY010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3201
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.212"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            597..929
                     /gene="traE"
                     /locus_tag="ACHAZB_002587"
     CDS             597..929
                     /gene="traE"
                     /locus_tag="ACHAZB_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFM8615205.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            1548..1889
                     /locus_tag="ACHAZB_002588"
     CDS             1548..1889
                     /locus_tag="ACHAZB_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFM8615206.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
     gene            complement(2126..>3201)
                     /locus_tag="ACHAZB_002589"
     CDS             complement(2126..>3201)
                     /locus_tag="ACHAZB_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFM8615207.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT         1027 a          477 c          660 g         1037 t
ORIGIN      
        1 ttttttgagt tataataata cgcgaagaga gataatttca ttatttaaac aagctaaaaa
       61 tcttgatatt acttcttttc aggcaatacg aatattattc gcttacattt tttgtataaa
      121 tttaattgtc tgctatgctt gtgatgaatt gattttcgtt ttgcactttt ggatgataaa
      181 tttggacaat tgaaaataca aaaaaaagaa aggcggtatg aactcggaag gctggtaacc
      241 ttcgtcagcg ttcatacacg gcattaagat ttggccctta atgcgttaac ctttcagtgt
      301 tacggtatta cccgcaatgg atttagtata acagatattc aaaagtgata ccactttttt
      361 tggctgtgtt aaggttaaaa atacgtgata ttacgatgaa tctatgccta agcataatgt
      421 tataaaaatg tccgtgtggc tctttgccgt atgacgaacg ctgaaattag agggaacgca
      481 tgtttggatg cgttccctct tttttgtttg tcatgaacga aaatcaattc tctatttttg
      541 aaaaataaca agaaaaactt gttaaaaata tttaaagaaa ttgggtggtt tattttatgt
      601 catatacagg aattttgagt ctcaaagata tttgtcacta cggtaaacgg tgtaccgcta
      661 cagagaaaat cacgaagaaa ctatcaactg gccaaagtaa aaccgttgtg cagtgtaaga
      721 aatatataat ccagaaagac aaagtctcag aggaaatgat ttattacatt gggaaacaaa
      781 agcaaataat tttaaaagat cctatcccct taaaagagct atatccaaca atcaaacatg
      841 tttatgacca aaacggtgta ctgattggtc gaagaaaaaa tggagtcttg cgttgtaccg
      901 caaaagggat ggggcgtttg atcggctgaa ttgatcattc tcataacttg tattttatcg
      961 tttttcgctt gcttgaaaat attgtcgaga cttctaaagg aacttagagt cactagaaaa
     1021 ataaggagta gacttgatct atcaacaatt cccttcgggg agtgacgggg taggttttaa
     1081 cgattggtaa cgtaaaaaat tggtcaatcg agtaaatgcg cttgtttgga tagtggtgaa
     1141 atgaatttgt gtaaataata agcgcagagt aaacaagagg gaatttagaa gttagacaag
     1201 ttagaatagc gtctagatta atcatgagca gctactaagg gatctagact ttgacgcaaa
     1261 ctcatggaat aaggagtagg tcttctcgta atggggagga cgcacaagaa attgtgtcgt
     1321 gtgctgcagt acatgtagat acttgcgagg acgttgatta ttggatcaat cgagcattta
     1381 ccgctgtcta ctagggtttc tcgtttcgga cgccataaac gagtcgaaat tttttatgta
     1441 tttttaagta aacgaaaaac gataaaatat gaacaagtcc tgaataaagt gaaaaaatta
     1501 atataaagca tagagaaaat gaaatttacg taatggagga atgacaaatg aaaaattatg
     1561 atccgaatat acgattgggc acacatacta ttaaggtttc tttccaacgt tgggattaca
     1621 aaggatttgt gacgtttaga aggggcggaa attgtaaagg attagatgtt ttagcattag
     1681 acgaggatga tttatacgat caaacattga ctgataatcc aattggattc ggcttgctac
     1741 ctgaagatga tgaaggtaat gaatggttta aaatgacgct tatgaatgat aatggagacg
     1801 aattatctgt tgaagatact tggtcatatt tgagtgatta tattgtcagc tttgaaatca
     1861 ttgaatttgt agcggataaa gaagaataaa aactggagga ataaatttta cagataaagg
     1921 cttagaagga gtcacgacga ttaataaaaa ggtagtatta ccttaggcat tgagtaagga
     1981 atttatttag tctaatggtg ataagtcaag atgaaattta agattttctt tattgagtaa
     2041 tatgttttga agcgcacgaa actagacccg atcaaaatca aatttttttc gggttgattc
     2101 aaggatttgc gtcaataacg gattgttatt tctctatcac actccttggt ggcgtataga
     2161 gttggtgttt acgtagtagc gtatccacca gacgcgtaaa ttttcttgcg gttaggacga
     2221 gtgcacgttt gtgttgatgc tttggaacct ccttatattt actttgataa aaagctttgt
     2281 attcaggaag ataatttttt acagagttgg cagcttcaac taagtagtat ctaaaatatc
     2341 gatttccttg ttttgtcaga ggtgtatttt gcgactgata gtttccagat tggttcactt
     2401 tccaacttaa tccagcgtat tttgccaatt ttgtctgatc ttcaaatctt tcgatttgac
     2461 caatttcagc gattaaaccg gcagcataga ctttaccaac gcctggaata ctggttaagc
     2521 attgatattc tggaatgaca acaattactt gttcgatggc tttatctaat tctttgatat
     2581 ttttttcaag tgctcgtatt tctcgtacga gaacacttaa aacaacatta attgattctt
     2641 gtgcaagagc acctaggcgg taactcatgg taattgcacg ttgaattgct ttagcgattt
     2701 tttctggttg cttgaaacgg ccttttcctt tttcttgaag taaatcacag aaagcttcta
     2761 aaggaagctc agcaagttca tccaatgtga aatcttccgt catgagtgaa atgagggttg
     2821 cactaaaaag actcgtcgat tcgtctcgca tttctcgagc aagcgtatta catttgtacg
     2881 ttagattttc aagaaaatgt tgctttgtac gaatcaattg cttaataagt tgatagcgag
     2941 tacgtgttaa acgctgtaag gccatgtatt tctcttcttt gataggggaa gttgtgaatc
     3001 gttgaatacg caagaaatca gcgattctta atgcatcgtt tcgatcagtt ttattctcat
     3061 caaacatgcg actaaattgt ttcacccgaa aaggattttc aatcgttaca agtgtgttga
     3121 gcttctttag tttctcatct tcatgaaaaa acatggaagg atggaagctg tacatggacg
     3181 ttgactccat gccaatcaca a
//
LOCUS       DAYYCY010000208         3180 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000208 DAYYCY010000000
VERSION     DAYYCY010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3180
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.213"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(552..2165)
                     /locus_tag="ACHAZB_002590"
     CDS             complement(552..2165)
                     /locus_tag="ACHAZB_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM8615208.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
     gene            complement(2306..>3180)
                     /locus_tag="ACHAZB_002591"
     CDS             complement(2306..>3180)
                     /locus_tag="ACHAZB_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8615209.1"
                     /translation="LVKKTLTYSIDTHLHLSAAPYFRLLINESANLYAEFAHKSIELD
                     NPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFSIDQEITSIITFL
                     KNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCLQKIQLSSLPRHY
                     QQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLLEQLAQIFFISPA
                     SLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGFHSIHHFSSAFKK
                     YTGLSPSKYYLSLK"
BASE COUNT         1027 a          548 c          562 g         1043 t
ORIGIN      
        1 ttaaataaat aggaacaaaa aaattaaatt atttaataca cacattaggg atcattcatt
       61 gaaggattag aagacaacga ggatggcgaa aaatatgatt acaatagaaa agtcgttcta
      121 aaccaatcag ttcctagtat cacacaacgc aagccagtcg atttggtgag attagaggag
      181 tgaaagcaaa ctttattata taagaaaaaa tattcgaaag ggatgctagt ttaggtacta
      241 gtgctgatag attaatatga tgcctcgtaa tattaggcgt agttactcta gagacatcac
      301 tatggactat gatttgtctc agagtcacaa catgtttttt tatgaaaaga aacagctaaa
      361 ataattaaat tgcgcaatgt ttaaactttc ttaaactata acctaataaa tgtgaagaaa
      421 taatgcacaa tttcttcaca tttatcttct attctatata cataccaacc aacaacccta
      481 tatacttttt catttttact cctcaagtaa aaataactcc tagaccgcgg ctacccaccg
      541 cggtcgtttt tttattcttc aatcaaaatc gctttgatac ccaaacctgc caagattcgg
      601 gcagaagtat aagtattcaa tcctcttctg aaagtaatgt aaactttttt atcttttggg
      661 atttcattga ttcgatctcg taactcattc attggaatat tttttgttgc cgagatactg
      721 ccagaaggtg ctttattagg ttcacgtata tctaaaaaga acgcttcttt caaatcgttt
      781 tcgggtatat cacttgcttt gatcgtttct acgatattcg tcatttgatt gattgcgaca
      841 taaccagcta tattcaacgg gtcacgggta gtagaatacg gtggagagta aggcaactcc
      901 aaatcaggca aatcaaagac tgtcaagttt ccggtgattg ctacagaaag ttctcccatc
      961 cgtttatcga cacctttttc tccaaatgct tgtccaccta aaatacgacc gctatctgca
     1021 tcaaaaatca gctttagatt taatcttgta gcttctggat agaaataggc atggtcaaag
     1081 ggagtgataa aaacagtttt ataatttgtt atgcctgatg cttttaattc atgttctgtc
     1141 attcccgcat aacttgctgt atgatcaaag atttttgcga cacctgcacc aatatatccg
     1201 cgatagcgca taggcgtacc atttaaaata tctgccagca tatgtccttg acgattggcc
     1261 gcactggaaa gcatactttg gatcggctga ccagtcacta cactagtcgt ttcgataata
     1321 tcgccaatcg catagatgtc cggcagattg gtttgtaact ggtcatcaac aatgatctga
     1381 cctgtatctg ataattgtat gccggttgct ttcaccactt cattcttcgg agaaacacca
     1441 acagcaaaaa ttagcatatc agcagaaagg acggagccat ctgataatac tatttctcga
     1501 cctttatcac ggatctcagt aactcttgtt tctaaatgaa cggctaatcc ttctttcagc
     1561 aatttgtcat aaactaaatt agcaatttct ggatcataag ggaaagcgac ttgtggcaat
     1621 tgatcgatta aagtcacgtc taagccgcga tgcttgaaat tctcagctaa ttccagaccc
     1681 ataacacctg caccaagaat gacgacatgt tgtggatttt tttcttcaag gaacgatttg
     1741 attcgatccg catccgtcac actacggagg acaaagccgt tttctgcttc aaaaactccg
     1801 ggaatatccg gataatctgg tctagcacca gaagagatga tcaacttatc gtaatctgtt
     1861 tttatttctt catttgtgga taggtcttta actgttaatc gcttagtaga aggatcgatt
     1921 gctgttactt cgtgtttagt aaagacatcg atgttgtttt ttgttttcaa tatttctggt
     1981 gtacgttcga tcagcgagtc acggtcagtg atcacacctc ctaaataata aggcaaagca
     2041 caacttgcat aagaaatatt ttctccgcgt tcatagataa tgatttcgtg cgcttcattc
     2101 aatcgacgga aacgagtggc aaatgaagga ccgccagcta caccgccaat gacaacaact
     2161 ttcataaaaa catctccttt tctacgttga tcgtactatg ttatggtaaa aaacagcaaa
     2221 taattgtgcc ggaaaattat tgtttatttt gaatagacag aaaatataga gaaataattt
     2281 gtggataaaa cctaaaaatt tttttctatt ttaaggatag ataatacttt gaaggggata
     2341 accctgtata ttttttaaaa gctgaagaaa aatgatggat cgaatgaaat cctaatatat
     2401 ctgcaatttc tgtataggtt aattcatttt ctagcacgag acgttttgct ttttccattt
     2461 ttagttcttt aaaatagcta ataacacttt ttccattcac ttttctaaaa tgttttttta
     2521 agctggctgg actgataaaa aaaatttggg ctaattgttc aagcaaaaaa ttttctgaaa
     2581 gatgttcttc taaatatcgt atgattggat caactaattc atctgaataa agaatatgct
     2641 tttttttagg aatgactttc tcatttgaag gtattgtcaa taaagcaagt tcctgttgat
     2701 aatgccttgg taaggaagac aattgaattt tttgcagaca ataagtaact aataaggaaa
     2761 gcaaatgaat gctcatctgt tggcatagta tgaaaaaaga attgatttcc tgatcatagg
     2821 aaatagctgg cttactatgt tttagataat caattttgaa ctgttcattt ttcagaaaag
     2881 taatgataga tgttatttct tgatctatag aaaaagattg tcccgtaata tgtgctaata
     2941 attgctcatc aaaataaaac tggataatga taaaagtttg tgtttccttt tgagtagaaa
     3001 agatttggac ttcttgctgt ttatcaatga ataaaaggga aggattatct agttctatag
     3061 atttatgtgc aaattcagca tataaattag cgctttcatt tattagtagt cgaaaatagg
     3121 gagcagcaga taaatgaaga tgggtatcta tggaataggt tagtgttttt ttgacaagat
//
LOCUS       DAYYCY010000209         3134 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000209 DAYYCY010000000
VERSION     DAYYCY010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3134
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.214"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(122..1057)
                     /gene="murB"
                     /locus_tag="ACHAZB_002592"
     CDS             complement(122..1057)
                     /gene="murB"
                     /locus_tag="ACHAZB_002592"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFM8615210.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1130..2050)
                     /locus_tag="ACHAZB_002593"
     CDS             complement(1130..2050)
                     /locus_tag="ACHAZB_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8615211.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQEADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2163..2915
                     /locus_tag="ACHAZB_002594"
     CDS             2163..2915
                     /locus_tag="ACHAZB_002594"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFM8615212.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
BASE COUNT          883 a          632 c          543 g         1076 t
ORIGIN      
        1 tatatttttt gttttattgt gttaaaatgt acaagatttg cgccatttgc gtaaacaacc
       61 acaaaaaaga gcaggacggc caccgtcctg ctcttttact tattttaagt tccattactt
      121 attaattggc taaacttctg cttgtttctg atattgcttg tttttctcca atgatccgca
      181 cttctgtttc taaagacaca tcaaattttt ctttgattac ttcttggatg tgcgcaatca
      241 attcaatata atctgtcgct gtcgcatgat cgatattgac gatgaatcca gcatgttttt
      301 cagagacttg tgcaccgccc catttcaggc cttgcaatcc tgcttgttgg atcagctgac
      361 ctgtatagtg tcctactggg cgcttaaaga cacttccgca tgaagggtat tctaatggct
      421 gttttgattg acgcaattcg gtcaactcat ccattcttgc tttgattgct tcataatccc
      481 ctgtttgcaa actgaatcga gcttccaata cgatagcacg taattcttgg actttactgt
      541 gacgataaga aaataccatt tcgtcttttg tcaaagtttt caatgttcca tcttcaagca
      601 aaagatccac ttcagcaaaa acatctttga tctctccgcc atacgcacct gcattcatat
      661 aaaccgctcc accgacactt cccgggatgc cgcaagcaaa ttcaaatcca gtcaatgatt
      721 ctgccaaagc tgcataagtc gtatcaatca gtgaggcacc tgcttccgca atgaccatcg
      781 ttcctttgac atgaatctgt ttcatttcag tcagcatgat gactacaccg cgaatcccgc
      841 catcttgaac gatcagattg cttgcatttc ctaataccat ccaaggtata tcatggattc
      901 ggcaataatc gatgatttgc tttacttctt cttgtttttt cgggaatgca aggacatcag
      961 ctggtccacc tgttttcgta aacgtatagt tcatcaatgg ttcatcaaac aatatagtaa
     1021 tcgtggctaa gctttcaact atttcttctt tattcactga ggattttcct ttcttttctc
     1081 aaacgtcctc attattctat cacgcttaat acaatcttgc cagtcttgtt caaagttgat
     1141 gacggtagtg atctttaagc acttcctcac ataattcatg acttcttaca acttcttttt
     1201 gtttaagcaa ttctgtttcc gatttaccta aacggatctg agaaaagaag gcctctacca
     1261 ttggctcaaa cccgcgtttg actagcgtgt ttgtccagtc gccaaattct ttcacttttt
     1321 ttccatcagc ctcttgaatg acaagctggg tcagatcgtt tacttcataa gtaccatttt
     1381 tacttgttac ttgatatttt tctatattag cgccacttgc taaatccatc gttaaaatag
     1441 ccgtttgatt ttgtgtttct aattgtaaaa tggcatattc caaaaattcc tttgtttctc
     1501 ttatctggta ttttgttctc aaaatgggtt catctaataa atacacagct gtatcaataa
     1561 gatgtaaaaa caaatcataa aggacgaact ctgtcgtttc ttttgccgct attcgatttt
     1621 tttgcaggtg gatcaaccgc ttatctggga tacgtttcaa ttcttctacc ataggagcaa
     1681 agcgccggtt gaaaccgatc atcaaaatta tttggttctt atcagctaat tcttgcaatt
     1741 cacgaacttc atttagatgg acactcagtg gtttgtcaat ataaacatgg ataccatttt
     1801 ctagacattt tttggctaat tcataatgta cttttgttgc tgcgtggatc atacatgctt
     1861 cgattttttc ttctaaaagc tcatcgatcg tttcatatag atgttggaac ccgtactctt
     1921 tttgaattct ttcttttgtt tctttatttc ttgttgcaaa ataaaaatca agtgttcctt
     1981 gttttttcgc ataagttggt aaatatgctt tttctgcgat attccctaca ccgattacac
     2041 caatcttcat ctactttcct cctggatcat ttagttactt tcatcttaca ccatttttta
     2101 ctaaaatgag gtacaatagt atcgcaaagg taaggaaatt aattcgaagg agtgtgcttt
     2161 tattgaaatt tatctcttgg aacgtaaacg ggcttcgtgc catcgtaaat aaaaattttt
     2221 tagaagtttt tcatgagtta gacgccgatt ttttctgtct gcaggaaacg aaactccaag
     2281 caggacaaat tgatttagat cttccaggtt attatcaata ttggaattac gcggaacgaa
     2341 aaggctattc cggcacagcg atttttgcta aaaaaccagc attgaatgcc acttacggaa
     2401 tgggtatcga tatccatgat acagaaggcc gtctgattac tttggaatat tccgatttct
     2461 ttcttgtaac ctgttacacg ccaaattctc aaagcgaatt aaaacgttta gattaccgat
     2521 tagaatggga agaagccttt tataattatt tggaaaatct aaaaaaacaa aaaccagtca
     2581 tcgtgtgtgg ggatctaaat gtcgctcatc aaaagattga tttgaaaaac tggaaaacga
     2641 atcaaaaaaa tgctggattc actccagaag aaagagcggc tctttctcga ctgttggata
     2701 acgggtttat cgatacattc cgttattttt atccaaccca ggaaggtgtc tactcttggt
     2761 ggaactaccg attcaattca aggaaaaaca atgctggttg gcggatcgat tactttttga
     2821 cgagcaaaga tttagagcca ggactagctg atgcaaagat ccatacggac attatgggca
     2881 gtgatcattg tccagtagaa ttagatttga aataaagagg ttgtgaaaaa gtcgtttagt
     2941 ttcgagtatt aagaaaaatt ttgtgaaaat ggctttccac attttgcata aatttggcat
     3001 aataccgaga aactggcttt tgaacactgt ttatcgagag attgtgaaaa agctgtcctg
     3061 catcaagtaa taagaacagc caaacaaaaa tagttcctca tatttttcgt ttggttgggc
     3121 ttattaccgc agat
//
LOCUS       DAYYCY010000210         3094 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.215, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000210 DAYYCY010000000
VERSION     DAYYCY010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3094
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.215"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            64..819
                     /gene="fabI"
                     /locus_tag="ACHAZB_002595"
     CDS             64..819
                     /gene="fabI"
                     /locus_tag="ACHAZB_002595"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFM8615213.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(929..1981)
                     /locus_tag="ACHAZB_002596"
     CDS             complement(929..1981)
                     /locus_tag="ACHAZB_002596"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HFM8615214.1"
                     /translation="MIKCGENNEGGKYMQIDWNNLGFSYIKTPWRFIAKWKDGQWEEG
                     ELTEDNYLTIHEGSPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLM
                     PMVPEKMFVDAVKQVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVF
                     CTPVGAYFKGGLKPTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCV
                     YLDPATHTKIEEVGAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIE
                     GDIFIDDLDQFAEAGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGI
                     QFGDIEAPEGWIEVVE"
     gene            2107..3027
                     /locus_tag="ACHAZB_002597"
     CDS             2107..3027
                     /locus_tag="ACHAZB_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HFM8615215.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT          899 a          624 c          590 g          981 t
ORIGIN      
        1 tagctattat tctctatttt tttacgacta tcaaattttt atcagaaaca aaggagagtc
       61 attatgtcat ttctagaagg aaaaaaaatc gtcattatgg gcgtcgctaa caaaaaaagc
      121 attgcttggg gttgtgctga agcaatgttg aaacaagggg cagaaattat ctatacttac
      181 caaaatgaac gaatgaaaaa atcattagtc aaattggtcg gcgaagaagc attcactgta
      241 gaatgtgatg tttctagcga cgaaagcatc gaaaaagcaa tgaatgccat cggtgaatat
      301 gctggggaga tccacggcct tgtccatgca gttgcttacg ccaacaaaga agaattgtcc
      361 ggaaatgtgt ctgatatttc cagagacggc tatcaattag ctcaagatat cagcagctac
      421 tctttaattg ctgtatcaaa atacgcacaa ccgtttcttg caccaaacag cgggatcgtt
      481 agcatgactt atctaggttc agaacgcgca atcccgaatt ataacatgat gggtattgcg
      541 aaagcttctc ttgaagctgc tatccgttat ttggcagctg agttctcgcc taaaggaatt
      601 cgggtcaatg gaatctcagc tggggcaatc aaaacattag ctgtcacagg tgttaaagac
      661 tatcaaaaac tgatccagtt atctgaatct cgtacgcctg atcagcaagg tgtgacgatc
      721 gaagaagtcg gtaatgcttg tgctttctta atcagcccac tttccagcgg aatcatcggt
      781 gatattatct acgttgacaa aggtgtccat ctttcataaa cttttgttgc attctttaaa
      841 gtaaaacata taaaaatcag gaaaaaactt tttaaagtct cttcctgatt ttttcttttt
      901 acgttttttt caactcatta ctttgaaatt attccacgac ttcaatccag ccttctggtg
      961 cttctatgtc tccaaattga atacctgtca attcatcata caaccgtttt gttactggtc
     1021 ctacttccgt ttcactataa aatacatgaa gattgttatt gtgataaatc ccaccgattg
     1081 gagaaataac agccgctgta ccacatgcac cagcttctgc aaattgatct agatcatcaa
     1141 tgaaaatatc tccttcaatt gcttgcagtc ctagtcgttc ttcagctaac tgcaacaagg
     1201 aatatttcgt gatacttggt aagatagagg gcgattgcgg agtgatgaat tttccgtctt
     1261 ttgtaattcc aaagaaattt gctgcaccta cttcttcgat tttcgtatgt gtggctggat
     1321 caagataaac acaatctgag taatccagtt cttttgcttc tgttcctgga agtaaactcg
     1381 ctgcgtaatt tccgccgact tttgctgcac cagtcccctt aggagcagca cgatcgtaat
     1441 cagatacaat aaaattcgtt ggttttagtc cacctttgaa atacgctcct actggcgtac
     1501 agaagacgac aaataaatat tctggagctg gatggacacc aatgttttca ccgaccccaa
     1561 tcagtaaagg gcgcaagtat aatgtagcgc ctgttccata gggcggcaca aaatcttcat
     1621 ttgcttttac cacttgcttg acagcatcaa cgaacatctt ttcagggacc attggcatca
     1681 gcaatcgtct agcactttga ttcatccgtc gactattttg ttcaggtcga aagagattga
     1741 ccttgccgtc ttttcgacga taagctttta gaccttcaaa acattgctga ccataatgaa
     1801 gcgctggaga gccttcatga atcgtcaggt aattatcttc tgtcaattct ccttcttccc
     1861 attgtccgtc tttccatttg gcaataaaac gccaaggagt tttgatataa gaaaaaccta
     1921 gattgttcca atcgatttgc atatacttac caccttcgtt attttctcca cattttatca
     1981 ttatccatat tatttgtcat tttattttca gcttttttcc ttcattatag ttatttttgc
     2041 tatttccttt tttgaaaact tcttggtaca atacagaaag aatcaaattt tagaaaagag
     2101 gattttatgt tttcattatt agcaactgcc ggtacgacag actcttctgg cgagacattg
     2161 acagatcaag ctgtcagcaa agtaaatgcg tttcagcggt tttgggacgg gattgattgg
     2221 gacgccattt tggctgcctt tatccaaaaa agtttatatc tgattttttt gatcgtcctt
     2281 ttttggattc ttcaccgtgt gggaaaatat ctgattgata agtcttttaa tcagtattct
     2341 aaaaaagcca ttctaaacga gagccgctta aagactttac atagtctttt aaatactatt
     2401 tttcattata cccttggttt cttttttatc tatgcaattt tatctgctat cggtgtgccg
     2461 atcggatcac ttttggctgg tgccggaatt gcaggtgtgg caatcggtct tggtgcccaa
     2521 gggttcatga gtgatgttat taccggattt tttattatca tggaacaaca gatggatgtt
     2581 ggggactaca tcaagctagc aaacttaacg attgaaggaa ccgtcgtatc tgttggtatc
     2641 cgtacccttc agctgaaagc aacggatggt actgtccatt tcatcccgaa tcgaaacatc
     2701 accacgatca gcaatctctc tcgtgcaaat atgcaagtac ttttagatat tcggattgtt
     2761 cctgaagaag gatatgacaa aatctatgag atcattgatc gagtaaatcg tacattagct
     2821 gaaaaatacc aagaagagct ccaaactgaa ccttctatct ttggattagt ggatcttgga
     2881 aacagcaatt ttgcgattcg tacggtctgc tacgcattga acggaaaaca atatgcctta
     2941 aaagaagagt tcttgagctc ctatgtcaaa gaattgacag cttctggttt cacgattccg
     3001 aacagtccaa ttgctgtcgg aaaataattt ggacaaacga tgcaacgaat ctcactaaaa
     3061 agagcgtggg acaaaagtaa agaacacttt tgtc
//
LOCUS       DAYYCY010000211         3058 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.216, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000211 DAYYCY010000000
VERSION     DAYYCY010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3058
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.216"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            241..2301
                     /locus_tag="ACHAZB_002598"
     CDS             241..2301
                     /locus_tag="ACHAZB_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HFM8615216.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRREESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2384..2953
                     /locus_tag="ACHAZB_002599"
     CDS             2384..2953
                     /locus_tag="ACHAZB_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8615217.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
BASE COUNT         1109 a          542 c          576 g          831 t
ORIGIN      
        1 agctgattta tgtttttttc aaagaaaagg taactagata taactagata taactagata
       61 tattacttaa caaaattaaa aagtacataa gttattattc cgattttctt gaaacaataa
      121 aaaatcaaaa aaaatactct cctaatagtc catcttcttt tgattttatt tatataatga
      181 aatcatctta tgaaaattac tacttcatgt ttagacaaga gtagggagga ctaagaataa
      241 atgtgttcaa aaagacgttc aaaacaacta aataaacacg cttgtcgttt agataaggaa
      301 cggtcgtgtt ctgaaaaaca ctacgttaag tttatgatgc gcacgatgct gatcagttta
      361 tctgtaggtg tctatatgca tgtacccgtt caggcaacga cacaaaccat caaggtcgta
      421 cgtacctctg tttctgagac cactgattcc cctatcacta ttactcatac tttcactgat
      481 gcaaatggaa aacagctact tcctgcattg acttcctata ataaagtcag ttatcctcca
      541 acgataaacc actactggtt aattcctaac aaaacattat ggacatcgga aaccggaagc
      601 agtgtgccgc tttctatctt ttccgatatg actgatccta caaaaggtga tcagttaaaa
      661 cagatcactg atattttgaa tcatgctttg acagatataa aggacggtgg aagcgcacaa
      721 gtttcttatg tatatgaaaa agattattca cagtttgaaa gaaagaaaat cgatgttctg
      781 gcagggacaa aagtttcgat atctgatctg gtagaaaggc tgaagaacgc agatggaaca
      841 caaggagata tgtccaaagt aatttcaaac aatgggaata tcgtggatac gactaataca
      901 ggaacatcta ctatttcctt gtcctatttt gatccaattg ctttaaaaac aatgactgca
      961 tctgctgtga tccatgttcg tgctgatcaa acagctatca aaggaaaaga tcttcactta
     1021 agaacaggag agagttacgc ccctaaagat cttgttgaat atgtaacaaa aagcgatgga
     1081 acttcaggaa ataaagaaga aatcaaaatg aacgggacag tagatactac aactccggga
     1141 gaatatcccg ttgtattaac ttatatagac tcgaaaacat caaatgtggc agaaacaaca
     1201 gctgtagttt atgtagaagc atctgctgct tcatcgactg tcatcactca ttttatagat
     1261 agtcaaacaa ataaagaact tcaagcagaa acacatatga gtagtgtaaa tcgtattcct
     1321 gatttgcgcc tacctgctga attttctgat cgagtgccaa atatagattt atcgacttat
     1381 gaacaaaatg ggaaagtcac ttctatttct gattggatgc aagcaaacgg attagacaga
     1441 aaaagtcact ggtcggagat ccttgaagta cttcactcag aactgatgaa taataggaag
     1501 caatcaggag gggaaattac ctttacttat gtttatatac cagacaatcg ttcattagaa
     1561 ggtcatgatc ttattgttca tccaaacgaa aaaataaaca aagaagagtt gatccaatca
     1621 gcaacaaatt ccttaggtaa acccgtaccg ttaaaggatg tatcggttac aatcaatgaa
     1681 gtaccattgg aggatggtta tttgaccaaa caaacaggtg attttacgat tatgtatagt
     1741 tatcatgacc cgtattcact ccaagaactc catgctcagt cactattaca tgttttacca
     1801 ttaaacaaac agatagatca aaaagaacaa aaaagacgag aagaaagctt gtcagaaaaa
     1861 aaacaacaaa tggctcatgc agtttctcca acagggaaag taaaacaaac aagaagccta
     1921 gagttagcaa gaatatctag aaaaatctca gaatctaata aaacgataag gcaagaagct
     1981 catttggccc cacaagtttc tggattggaa aatgaaattt tagagcctaa aataaaacaa
     2041 caatcagaaa aagaatttgt tgctaaacag tcggaagatc agaaggaatc agatgaccag
     2101 aaggaaacga agaaaaaacg cttgtcaact attgattcca ccgatgagca tctgcagccg
     2161 caagtaccac ttcctagtcc gggaacaccc gcacctggtg gaggtattcc ttcttctgct
     2221 ttaggagatt tcatgcgagg tatggcagga acatgggtat acgctaatcg aaatgaattt
     2281 gacgcagatg actggttctg atttatatct ctatctgtta tagtcaaata agtagctgag
     2341 tcatatgtta tttggcataa tggagacgag gagggataga aaaatgttag ataaaacgtt
     2401 accttacgca gaaatctgga tgacaagacc acttcaacaa aatgttccaa tcacgccttt
     2461 agcggaaggt tttgaatttc gattttatca aaaagaagat gaatctgctt gggcggagat
     2521 tgagacttcc gttggggaat ttcaaacgac agatgaagca cttgagtatt ttcaacaaaa
     2581 ctttgctcct tatcctgaag aacttgaaag aaggatgttt tttgtagaga caaaaaaagg
     2641 agaaaaagta gcaacctgta ccgcttggcg aaaaaaatca agagagcaag tgtatccggt
     2701 ttttcattgg ttagcagtga aaccagatta ccaaggaaaa gggctcgcaa aagcattgac
     2761 tgccagagtc ttgcaagaat ttcctaccct ccatacggac ggaccaatct atcttcacac
     2821 acagacatgg agccatcagg cgattgctct ttataaaaaa atgggtttta cttttattgc
     2881 tgaaaatcta gacggcacac ctaatccaga ttatgaaaaa gttatgcagg ttttaagtaa
     2941 attagataaa taacgaaatg gtgaaataag tcctcagtat tgggataaaa aacgaaatag
     3001 tcattcctca tatcttactt ttttgagtta atactatcgg ataactaatg gcatgcca
//
LOCUS       DAYYCY010000212         3052 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000212 DAYYCY010000000
VERSION     DAYYCY010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3052
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.217"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..352)
                     /locus_tag="ACHAZB_002600"
                     /pseudo
     CDS             complement(1..352)
                     /locus_tag="ACHAZB_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(612..1160)
                     /locus_tag="ACHAZB_002601"
     CDS             complement(612..1160)
                     /locus_tag="ACHAZB_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8615218.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(1161..2018)
                     /locus_tag="ACHAZB_002602"
     CDS             complement(1161..2018)
                     /locus_tag="ACHAZB_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFM8615219.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(2304..2590)
                     /locus_tag="ACHAZB_002603"
                     /pseudo
     CDS             complement(2304..2590)
                     /locus_tag="ACHAZB_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2580..2909)
                     /locus_tag="ACHAZB_002604"
     CDS             complement(2580..2909)
                     /locus_tag="ACHAZB_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFM8615220.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(2986..>3052)
                     /locus_tag="ACHAZB_002605"
     CDS             complement(2986..>3052)
                     /locus_tag="ACHAZB_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFM8615221.1"
                     /translation="KVDEDEHVVEIYSARTHYESQ"
BASE COUNT          892 a          620 c          384 g         1156 t
ORIGIN      
        1 aatgttctta ctgcctcttc cacactaatt tcttcgttca atttcactaa taatacttga
       61 tctcgcttat tataatcgct ttcatttatc ctaagagtca aatctttttc caccataact
      121 tttacaccca ataaattttg cggtttattt ttctctgttg aaattctttg tgtagccttt
      181 aaactgacaa ttagctcatt gggtacatac ttatttgaga gtcatctaat tggctcataa
      241 tcctttcttt ttggttagta tctgcagtta cttgtttagt atgagccatg aacccaaaca
      301 tactcacccc aacaaccaat cctaaatata ttttcttttt aaactttttc atctacatct
      361 cctcctaata gttttatttt agagttagct ctattactta tttactaact caacaacaga
      421 ttatcataca cttgttaatg aaatcacaat atctcattaa aacacaccct aaaatcataa
      481 ccgttcctat gctcattttt tggatttttt aatcacgtaa ccagaaaaat aaatcccctc
      541 tatttcacgc aaatcaaaga agaactaagt aactaccgtt taaaggtagt tacttagttc
      601 ttctttgatt attatctaac tttattattc attccccacc ctttgcgcag tgagtcttcg
      661 gtatcttgtt ttatctgact ggtagttctt attatttctc cacgatcatc taattctgga
      721 acaattggat atttttctct gaacaagtct agttctttca ccacgtcatt aaacaaatct
      781 tgcttatctt cttgcgtaag attctgttta atatactcat ctaaagcttt tctaagcaat
      841 tcatctcttg tcatattagt ctcatacttt tctactaaaa ctctggaagt cgcatttaac
      901 aaagtcgaca gttttaaagg atgcttggtg gaataaacct tatcaaactt tctgattggt
      961 gcgccaactt tttttgttct tcctctactc tgtctttcaa cattggagga agtttttatt
     1021 tcttgaactt ggtgcacttc taattgctta ttttcagatg gtgtaattgc ttgctgcttt
     1081 ttctttgctt ctgcttcttt ttttattttg gcgagtcctt tgttgttccc aaaaatatca
     1141 agtcctttga tgtcagccaa ttactccacc tccgcatacc aattctcgtg ttcttccaat
     1201 tctttcaaaa tactgataaa taattcgtgg cttttgcgat cccattgttt taagagacct
     1261 ttctttgtct tctccatcgt aatacctgat ccgtcgtatc gttttaagcg cgcatttttg
     1321 agtacaacat ttttaatcaa atgttcccca taaatctctt gggcttgttt atacatttcc
     1381 tgatctactg cgtcaccgct ttccaacatg aaaggaataa caccagcaac atataaattt
     1441 gtatcaaatt ctttcacaaa aaagtccata tactcaagat attgcatcgc atttctcatc
     1501 gattttactt gggtctgtaa gacgacgatt gcccaatccg caatgtacat cgcggattta
     1561 ctatatacag aaatagaagg tgggacatct aaatagatga cgtcaaactc atcaaataat
     1621 ggttctagct tttctttcat atatttgaat tgggataatg aatcattata ttggtctttg
     1681 atccaatcat catacatttc aaaggacatg tctgctggaa gtatatagag gttattgatt
     1741 actttataga cagcctcttt aggtggaaga ttgtttatca acacgtcagt cacagtattt
     1801 tcgtaaacaa tcggttcacc ttctccgcct ccttcttcgt tagtgaagtt catatgcgtt
     1861 aagttcatca catcagaagt atttccttgc atatccaagt caattatcaa ggttcttttt
     1921 ccttcattaa ctgctgattc aaaccctaac atttgcgcgc acgtactttt acctacgcca
     1981 cctttaaaat taccgattac atatttcttc gctaccacgt tcctacctct tttcttttag
     2041 caatattcat ttgatactat tataacatat ttatttttag ttactaccaa aaaaaagata
     2101 tatatgtact accttataat taataagtaa aaggtagtac ataaataagg aaaagctgaa
     2161 atatattgat ttatagcttt cctcaaagta atgacgttat ttaatttatc gagaagcaga
     2221 gaatactcgc gactttagtc atgtagacga atcagatttt cgataaaaaa gaagccgtcc
     2281 ttcttaagaa cgacaacaaa tcactagatt aattttttca actgttcata aaagttttca
     2341 tgcgttccac ataacaatac tggaataacc ttgttttcta taatctcgta agctactcta
     2401 tatgttgtgc ccgcatattt gaacccacta gaccaaatac ctgataaatc cccagttttt
     2461 tgttcaccct tatatggatc aatcgcaatt tcatcataaa ttaaattcaa aaatttttct
     2521 ttcaatggct tctcttttag ctttttaaat acttaatgac atgttttgaa ggaacgattt
     2581 cataagccaa tttctgttgc caactcctct ttagtcatta ctttagaatt agtaagtgta
     2641 tcttcagcaa tatctctaaa ggaagaatgc attttgtttt ttctattctt aaattctaca
     2701 agaatctcat ctttatcgta gccttctgca attatatcag acaaaatatc ttcgctaaaa
     2761 tcataaaatt ctttttgtgg ttcaacaaat tcataaagaa taccattctc taccaacttt
     2821 gcttcaactt cgactccatt aggaacatta aaatctttag gtactgtcaa catgattgaa
     2881 ttgccttgtt ttcttgtttt tgtaatcatt taaatccctc atttctaaca gtatgtaatt
     2941 acttagtaaa tacattatac cacgagataa tgtatttaca agtaattact gtgactcgta
     3001 atgcgtcctt gctgaataaa tttcaaccac gtgttcatct tcatctacct ta
//
LOCUS       DAYYCY010000213         3035 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000213 DAYYCY010000000
VERSION     DAYYCY010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.218"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..113
                     /locus_tag="ACHAZB_002606"
     CDS             <1..113
                     /locus_tag="ACHAZB_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM8615222.1"
                     /translation="SVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            complement(184..1494)
                     /locus_tag="ACHAZB_002607"
     CDS             complement(184..1494)
                     /locus_tag="ACHAZB_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HFM8615223.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     INFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1506..2924)
                     /locus_tag="ACHAZB_002608"
     CDS             complement(1506..2924)
                     /locus_tag="ACHAZB_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8615224.1"
                     /translation="MLSQNAVTANTVKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
BASE COUNT          959 a          627 c          468 g          981 t
ORIGIN      
        1 ccagtgtcag caatcaacgt aatcatattc caagaaaatc attgaattac agaacaccaa
       61 tcgaaatatt tttgagctat gtacaagaag cattttattc tagcttaatt tgacaaatca
      121 tatatcatta aaaaaaataa aggtcaagct aaaatattat ttaagcttga ccttattata
      181 atttcaacga ttgtcaaaag tattgtttgc agctgtgttg ccagtttcta tagataaatc
      241 acagcttttg aaacataagt tttgtactgc ttcgattcgc ccgaattctt ttgcagttat
      301 ttggcaatga gaaaagcaga catcttctat aggcttttca ggaaatgcta ctaaatcaaa
      361 agctcgggaa ggaagtgagt aacctggaga aaggcatgcc tgtacgtttt tgatgtgtat
      421 gtctttgact ttcgtcattt gctttgattc aggtatttct tcagccactg cttgccaact
      481 ttctggcatt tgagcaaggt cattgctatt ttttctattg tattcattat gccagtccat
      541 tatccaagaa aaaggaaatt gtacatcaat catattcaga ttttctaccc tgatattttc
      601 gatcactccc cctctttctt ttgaagattt cattcgaata ccacagtctg actgaaaaaa
      661 attgatatcg tgaatatata cgtcactaac tccagcacta acttcgctac caatagttac
      721 accgtatcct gaacggattt tacatctagc aacctcgatt cttgatgatt ttcgattgac
      781 tcttgcacca ttgccatctc tgccagactt aatagcaata caatcatcac cacaggccaa
      841 atcacattca tagacacgca catttgttga agaatcaata tcaatcccat ctgtacttgg
      901 tccatcattg ttgcggatga ttactttttc tactaataca tcgttagaat aagtgatttg
      961 caagttccaa aaacctgatt tctgaaaaat caaatcacga acaacaacat gttctgcatg
     1021 atacagtaaa caagcgcgtg gacgtttaat agcataatcc gcaatccaac gaagtccctt
     1081 ttgatcgtat tcagctcttg tcccgctttt ttgatctttt ccccaataca gctcccacca
     1141 atggtctcct tgaccgtcga ttattccttt tccttctata agaatatctt tcgccgagag
     1201 aacattcaaa atcgctgctg gccatttcat ttctacgccg gctactcgtg tatcgatttc
     1261 tggaaatgct tcgatatctg ttctcccctt gaggactgca ccttcttcaa agataagatg
     1321 actcttatct tttaaaaaaa gggctccgac taaataaatg ccttttggaa taaatacagg
     1381 taagtgttct ttcgcacctt gatcgattgc tttttgcagt attattgtat catcttgttg
     1441 agtgtcgttt gttttaaagt tcaaagcatt gataatccct ttttttgtcc gcaatacgtt
     1501 tctccctatc tttcattgat ttttttcaat tcttcaccaa taaatacagt ttttgaacga
     1561 ttcagtgggt aacctactag caaaatgatt gcggatacaa ataagcagaa tgctggtaac
     1621 aagtttgcca tcgtataaat cctttcttgt actaaaactg attggatggc tccacctgtt
     1681 gtagacgatt ggtaaccaat gagtgccagc ataaatccgc ctaccgctcc tgcacatgct
     1741 tgtcctactt ttctcgcaaa agaatttact ccatatactg ttccatcttc tcttaagcct
     1801 gtcttatatt gatgataatc aatcacgtca gtaatgaatg cccagaccat caaattgaat
     1861 aaaccagatc ctaaagtagc gataaataaa agaatcagat atacccatgc atttgttata
     1921 tgaaggaaat acatcagcaa gtacatacat gaagaagcaa gtaacgcaaa tacgcttgct
     1981 tcctttttac caaataattt tgtcagcttt gtagcaaatg gtgctaaaac tactactgtt
     2041 ccgtaagtaa ataaaagagc aacagacatt gcttgtttat tatggaagta atcattgaat
     2101 aaataagtca tattcgtccc agctaaaatc tgattgatca caatgaaaat gtcaacaatg
     2161 actaaaataa tcagtgctcg attagctaac aaacctttta ccaactgaga agcagatact
     2221 ttttcgtttt tgacaactct tactctttca gtagtcaatt tacaagttaa catatatgcg
     2281 acaaatgcta ttgcagcaca tccgattgaa atccaaaaga aatgattccc tgaaacgact
     2341 tgttgtccac tcttgttatg ggtatacatt aaaatcggaa tgaagaatcc tgtagttgat
     2401 ccgccaatcg cagaaccaat gcttcggaac gtggacaacg aggtgcggtc attaggattt
     2461 gagctaattg ctgctgccat tgctccatac gggatattga ttgttgagag aaaaatccca
     2521 taaacaatgt atgaaacaaa aatataagca atcttcaccg tcattggaag atgttggacg
     2581 aatggcaaaa acaaaatgac agtcactaag caaaatggat acttcatttt tctgatccaa
     2641 ggaataaatc ggccagtttc tgttaactct gagacatcac ataatcttcc aaccatcaca
     2701 tcagcaaacg catccaaaat tctcgaaatc aagaataacg tacctacgat tgcccctgaa
     2761 acgccaagaa cattcgtata ataaatcatc aagaaactat tgactaaacc taaaataaaa
     2821 cagttaccta gatcaccgaa catgtagcct atcttatcgg aaatcccaaa ttcctttttt
     2881 tcttttttta ctgtatttgc tgtaacagca ttttgtgaaa gcatttgaat tcctcctacc
     2941 tcttctagta aacgttttca aaattataac tctttttttc ttgtttttat attcacaatg
     3001 ttggcttttt attgcattat cttgacagat aaaaa
//
LOCUS       DAYYCY010000214         3025 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.219, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000214 DAYYCY010000000
VERSION     DAYYCY010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3025
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.219"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1243
                     /locus_tag="ACHAZB_002609"
     CDS             <1..1243
                     /locus_tag="ACHAZB_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM8615225.1"
                     /translation="KTKDQLNEFFANVLETPYWYAKKILSLLSECLDQPVYEEDIFFI
                     TLYFYSLNVTNVKTGIKSIVLAHGYSTASSMANVANRMLNKNVFQAYDMPLDITLEKI
                     EAKLIQFLGDYRTDSGLILLVDMGSLNQLGGLLVNHLNGPLLIIDHINTPLLLEVGQQ
                     VLNNKSITEIGAEIETSLTFKKQLLLPKKKKKKAIITCCYTGIGSATQIQEILTKCLK
                     DAVRELVIIPYDYKKLLENKNFETPFQLYDVIMIVGTEDPKINQIPYIGLDRLINGGS
                     IGEFVHYLEKELTIEVNDLQKELIFNFSISKIVENLTILDPDKVLSFVQKAIKELSKL
                     LNFDFSNNQLFLLYIHCSCMIERIVRKESVDEQEDIEEYMQANGEIMDKIHSAFFEIE
                     REYTIEIPDLELRLLSDIVQG"
     gene            1256..1888
                     /locus_tag="ACHAZB_002610"
     CDS             1256..1888
                     /locus_tag="ACHAZB_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8615226.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            2096..2320
                     /locus_tag="ACHAZB_002611"
     CDS             2096..2320
                     /locus_tag="ACHAZB_002611"
                     /inference="COORDINATES: protein motif:HMM:NF015692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFM8615227.1"
                     /translation="MQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEVI
                     PENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            2422..2655
                     /gene="relB"
                     /locus_tag="ACHAZB_002612"
     CDS             2422..2655
                     /gene="relB"
                     /locus_tag="ACHAZB_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HFM8615228.1"
                     /translation="MSQSTITFRIPDDEKELVLEYAKAHNSSLTELYRNAVLDKIEDE
                     IDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            2645..2914
                     /locus_tag="ACHAZB_002613"
     CDS             2645..2914
                     /locus_tag="ACHAZB_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFM8615229.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
BASE COUNT         1073 a          443 c          607 g          902 t
ORIGIN      
        1 gaaaaccaaa gatcagctta acgaattttt tgctaatgtt cttgaaaccc cttattggta
       61 tgcaaaaaaa atcttaagcc ttttatctga gtgtttagat caacctgtgt atgaagagga
      121 cattttcttt attacgctat atttttatag tttgaatgtc acgaatgtta aaacaggtat
      181 taagagtatt gtgctagctc atggttattc tacagctagt agtatggcga atgttgccaa
      241 ccgtatgctg aataagaacg tttttcaagc ttatgatatg cctttagata ttacattaga
      301 gaaaattgaa gcaaaattga ttcaattcct tggagattat cgaacagatt caggcttgat
      361 tttattggta gatatgggtt cactaaacca actaggcggt ttattagtga atcatctaaa
      421 tggaccactg ttaatcattg atcatatcaa tacaccgtta ttattggaag tcggccaaca
      481 agtattaaac aataaaagta tcaccgaaat aggagcagaa attgaaacaa gtcttacttt
      541 caaaaagcag ttactacttc ccaagaaaaa gaaaaagaaa gccatcatta cttgttgtta
      601 tacagggatt ggtagtgcga cccagattca ggagatttta acgaaatgct tgaaagatgc
      661 agttagagaa ttagtaatta ttccgtatga ttacaaaaag ctgttagaaa acaagaattt
      721 tgagactcct tttcaattat acgatgtcat catgattgta ggtacagaag atcccaaaat
      781 taatcagatt ccatatattg gattagatcg cctgattaat gggggaagca ttggcgaatt
      841 tgttcattac ttggaaaaag agctgactat tgaagtgaac gatttacaaa aagaattaat
      901 tttcaatttt tcaattagta aaattgtcga aaatttaacc attttggatc cagataaggt
      961 gctatctttt gtacaaaaag caattaaaga attatcgaag ttattaaatt ttgatttttc
     1021 taataatcag ctgtttttgt tgtacattca ctgtagttgt atgattgaac gaattgtacg
     1081 taaagaaagt gtggatgagc aagaggacat tgaagaatat atgcaagcaa atggagaaat
     1141 aatggataaa attcatagcg ctttttttga gattgaaagg gaatacacaa ttgaaatacc
     1201 agatttagag ttgcgattat tgagcgatat tgttcaagga taggagtgaa ttcgaatgac
     1261 aatgggagta atttttgata tggatggggt actcatagat agcgagacat tttattttga
     1321 gagaagaatg aacttctttc aagaactgga gatagatcca ggatcaaacc agattgatga
     1381 ttatgtgggg aaaacagaag ctgcaatatg ggaaacatta gttcctgagg acaaatcatt
     1441 aagagaggaa ctttatccga aatatgtaga ttatcgaaaa acgcatccaa ttgatttcgc
     1501 taaagcttta cgaaaagaag tgaaaccgtt acttgacttc ctcaagaagc gggatatacc
     1561 attggcactg gcatcttctt ctccagctgt cgagatagag agtatgctca ttcaatgtga
     1621 acttactgaa tatttttcct ttgttataag tggtgagtca ttaaaacaaa gcaaaccaca
     1681 tcccgaaata tatttaaaag cgaaacagca attagactgt gacttcttta ttgcagtaga
     1741 ggattcaacg attggtatta aatcagcaaa ggcagcaaat atctatacgg cggcactaaa
     1801 acaaccaatg caaatcgatc agagtgaagc agacatacaa attgaaagtc tacaagaact
     1861 ggaacaaatt gttttagaaa aaggttagtg aattaatgga aataaggcta tatatctgtg
     1921 ctagctattt tgactaacca aaaaaacttg ctataattca ggtgtagtaa aaggtgctgg
     1981 taacaccttt tacgaaaatg cttcggcagt gataatggga ttggcttttt aagtttttta
     2041 ttttgttcgc aatccctaag gtcatgtgaa tgatcctagg gattgctaga atattttgca
     2101 acagaaccaa aatacgaata gttatcaagc agccatattg actgcggtca atttaggagg
     2161 ggatactgat actactgctg ccgtaactgg tgctcttgcg ggtattcttt acgggggtga
     2221 agtgatccct gaaaattgga gaaaatcgct tcaaaatgac cagcttctta aaacgatcag
     2281 tcagagattt tgcgataccg tgctatctca aagaatttga ataatggata tgatgcaaat
     2341 cttgtttgta ctaacgttgt attgacaaaa aggattttat ttagtacaat gttagtacaa
     2401 atgatgaacg gaggtagaat catgtctcaa tcaaccatta catttaggat tcccgacgat
     2461 gaaaaagaac tggtattaga atatgcaaaa gcacacaatt cttcattgac cgaactttat
     2521 cgaaatgctg tactggataa aatcgaagat gaaatcgatc tacggacttt acggagtgca
     2581 attgaagttt ccaaagagaa aaaagagagt ggtatttctc aagaggaaat ggagaaactg
     2641 ctaaatgaat tataaagtca tctacatgcc gcaagcccag aagcaattaa aaaaaatgga
     2701 cagaaaccaa gctaaaataa ttgtcgcctg gatcagaaag aatttagaaa acacgactga
     2761 cccgcgtcag cacggaaaag gtttgactgg aaataagagt ggggaatggc gctatcgaat
     2821 tggaaactat cgaatattgg ctaatatttg tgattctgaa attgaaatcc agatattctc
     2881 aattggacat agaagtacaa tatataaaag ataaccgttt ttggaacgaa gtagagtttc
     2941 gaaagtcaat gtataatctt ttgaattttg cataatacca caggtagaaa ctacccgata
     3001 ctatcggttg gtttctgctt tttta
//
LOCUS       DAYYCY010000215         2991 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.220, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000215 DAYYCY010000000
VERSION     DAYYCY010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2991
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.220"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1392
                     /locus_tag="ACHAZB_002614"
     CDS             <1..1392
                     /locus_tag="ACHAZB_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFM8615230.1"
                     /translation="KVEVGGTVDLTDNVTNLPALPEGTTVTDVTPGGTIDTNTPGNYE
                     GVIEVTYPDGTKDTVKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNVTNLPAL
                     PEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNADKYNPE
                     GQKVTTDLNKEPDASEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTYPDGSSD
                     EVEVDVTVTDTRSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVD
                     VSTAGNKKGTVVVTYPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTGKTEPNA
                     EVTVTLPDGSQHHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNK
                     KDSGKNGSKGSKTDNQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGLILLGTL
                     GLLGYKNRKKENE"
     gene            1526..2869
                     /locus_tag="ACHAZB_002615"
     CDS             1526..2869
                     /locus_tag="ACHAZB_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM8615231.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
BASE COUNT         1187 a          403 c          573 g          828 t
ORIGIN      
        1 aaagtagaag ttgggggcac agtagatcta acagataatg ttactaactt gccagcgtta
       61 ccagaaggaa caacagtaac agatgttact cccggcggta caattgatac caacacacca
      121 ggtaattacg aaggtgtcat tgaagtaacg tatccagacg gtacgaaaga tacggtgaaa
      181 gttccagtag aagtgacaga caatcgttct gacgccgata aatatgaacc aacagtagaa
      241 ggcgaaaaag tagaaattgg aggcacagta gatttaacag ataatgttac taacttgcca
      301 gcgttaccag aaggaacaac agtaacagat gttactcccg gcggtacaat tgataccaac
      361 acaccaggca attatgaagg tgtcattgaa gtaacgtatc cagacggtac gaaagatacg
      421 gtgaaagttc cagtagaagt gacagacaat cgttctaatg cagataaata caatcctgaa
      481 ggacaaaaag tgactacaga cttaaataaa gagccagatg catctgaggg aattaagaac
      541 aaagaagatt taccaaaagg aactacttat gcttggaaag agaaagtaga tgttagcaca
      601 gctggaaata aaaaaggtac tattgtagtg acatatccag atggatcatc tgatgaagtt
      661 gaagtagacg ttacagtgac agatactcgt tccgatgcag ataaatataa tcctgaagga
      721 caaaaagtga ctacagactt aaataaagag ccagatgcat ctgagggaat taagaacaaa
      781 gaagatctac caaaaggaac tacttatact tggaaagaaa aagtagatgt tagtacagct
      841 ggaaacaaaa aaggtactgt agtagtaaca tatccagatg gatctaaaga agaagtagaa
      901 gttactattt ctgtagtaga taaaaaagca ccgaataaac ctcaagttga tcctattaca
      961 gagggtgacc aaattgttac tggtaaaact gaaccaaatg cagaggtgac agttacatta
     1021 ccagacggaa gtcaacacca tgggacagct gataaaaatg gtaattttac agttaaagtt
     1081 cctaaattag aggcagggac aaaagtgata gtaactgcaa ctgatgaatc tggtaatact
     1141 agtgaaccta ctaatgtagt tgtttcttcc aacaaaaaag acagtggaaa aaacggtagt
     1201 aaaggtagta agactgacaa tcaaggtagc aacagcaatc aagacaaaaa tagaggcaaa
     1261 agccagtcct caaaagtttt tcctaagacg ggagagagtg acagtaatat ctttactatt
     1321 agcggtggtt taatactgtt aggaacgtta ggattattag ggtataaaaa tcgaaaaaaa
     1381 gagaatgaat agaaaatcaa tttcttaagt cttctctgag aagaggaaga atctttttct
     1441 tcctcttttt gcttttttat gatgggaaat aagtgttagt taaagatgaa tatacttttt
     1501 atagaaagga aaagaatgta attaagtgga aaaaatagta attattggtt caaaccattc
     1561 agggctagcc gcagctcgat ttctaaactt aagcgattca aattatgaag ttattctaat
     1621 cgataaaaaa tcagaaatta gttacttgga atgtgggaca tcgttattta taaaaggaga
     1681 agtaagtaat ttagaagatc ttttttatca aaatagaatg acactaaatt ctgagataaa
     1741 aaagttattt ctagagacag aagttgacaa aattgatatt caaaataaaa agattagttg
     1801 tgtaaaaaaa gatggtacaa gattctctca agaatatgac aaagttatat tagctacagg
     1861 ttcaaagcaa attatgctag atataccggg gaataattta gctaatattt tttcgataaa
     1921 gaattttcca aatgcaaaag agatttttaa agctttggat gatagtacta ttaaaaaagt
     1981 aggaattatt ggagcaggat atattggagt tgaaattgct gaagccataa gaaaaagagg
     2041 gaaagaagta tatcttttcg atgttgcaga tagagtgttg tcgacttatt acgaccgtag
     2101 tttttcagat aaagttgaag aaatactatc aaaaaatgga atacatcttt gtctatcaga
     2161 aagtcctaaa aaatatttag gaagaaaaaa gattgaaaaa atagtcacta caagtggtac
     2221 ccaatactct atggatatgg tcgtacaagc tataggattt ctatctaata gtccaattgg
     2281 ggaaaaggaa ctcttgagag atgttagtgg agcatatcta actaatgaat accagcaaac
     2341 aaatataaaa gatatatatg ctattgggga ttgtgctaca acttttttta cgtccattaa
     2401 tagaagtagt gttgattttt ctatttctaa tgctctaaga acagcttata ttgcaactca
     2461 acatattaac aatcaagatt ttacaccttc tggttcccaa tttacaaatg gcttttctat
     2521 atttaattac aatttttacg cagcaggttt gaatttaaaa acagcacgat tattaagtga
     2581 gtctatagat tatatagaaa tagaagattt cattagacct gcttttttga gaaaaaatag
     2641 tcgtgtaaaa ttaagaatta gctttgaaaa aaatacaaag agaatagtag gtgtacaact
     2701 ttgtagtcaa gaagatttat ctggactatt atcaatgttt tctcttgcta tagaagaaca
     2761 aattacatta gacaaattga aaatgttaga ttatttattt catcctagtt ttagccaacc
     2821 ctataatttt attttaaagg cagtaacagg attaaacatt gaaacataat cgttactaaa
     2881 tgaaatcaag aaatatactg tattactcaa ttcattgaaa atatttatca taatttttaa
     2941 tagaaaacac tattaaagag aaaaaagaga ggtaaatcaa atgaaaaaat c
//
LOCUS       DAYYCY010000216         2982 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.221, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000216 DAYYCY010000000
VERSION     DAYYCY010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2982
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.221"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(199..813)
                     /locus_tag="ACHAZB_002616"
     CDS             complement(199..813)
                     /locus_tag="ACHAZB_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM8615232.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1419..1796)
                     /locus_tag="ACHAZB_002617"
     CDS             complement(1419..1796)
                     /locus_tag="ACHAZB_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8615233.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(2096..2977)
                     /gene="galU"
                     /locus_tag="ACHAZB_002618"
     CDS             complement(2096..2977)
                     /gene="galU"
                     /locus_tag="ACHAZB_002618"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFM8615234.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT          906 a          624 c          467 g          985 t
ORIGIN      
        1 ataactgtga aacaacttat aaaaataaca aactttaaac aaaacaagct taaaatcaaa
       61 gatgatccat cttaaaataa ctaaaacaaa aaaagtagtc gttgttaatc ctcattaata
      121 atcgttttct ttttttcaaa cgctccattt ttatgtggaa cattattttt ttaaggaact
      181 ttgtcaacat gtggataact attttccact tgtgtcctgg actagctgtt ttgctgtaaa
      241 atacaaaact tgctgcagca aaaagatcgt tgcaagagag gctgttccgc agatcgctgg
      301 atctagattt ggcaaagtca agactgcatc atgtaaattg gcgtggttgt ccaaaaccaa
      361 atcagcaaat tcaaataatc gcaaaccact gctatgttgc gatttgatac ttcgtgacaa
      421 atcaaatcca gtaacaatga ttaatggatg accattttct ttgatccact gagctagttc
      481 gatgattgcc gggtttcttc cttcattcga aagaagaaga aaaatgtctt ctgatttgac
      541 agaaagctga tcaataattg cctgtcctgc accttctagc gtttcataga cacctccggc
      601 tggattttca atcaatatac ttggtacaag atccggggtt tgatcccaaa attcaaatgc
      661 tacaccactt agtgtgcggc tagcatatat ctgcaatcta ccaccttgat taatttgttt
      721 cgcgatccaa gtgccagctt gaagcatcag cggctcttct atccttaatt tctcttccaa
      781 ttggtgataa tagtcaaaat aacataaact catttatctc tcccgcctag ctatacttga
      841 catgaataga aacagctgcc taatctaagg aagtacataa aaaaacagta gttagacagt
      901 aacacatgtg gtctttgctg tttcaaacac aaaaaaggca ttgaacgaat acgcccaata
      961 ccttgacata gcaaataaaa caacgtgcag accatggaaa atatctacat aggggtagaa
     1021 ttttcaccct agattaggta caataaatta ggtcgatgca ctgcattgac tatggcaaaa
     1081 tgatcggtca gggtgtatgt gtgtgactgg tcattccctg attcttagcg ccaactaagg
     1141 gtcagggagc ctttttttat tcagctgttt ctatctgaat ttattgtaag taatctctta
     1201 tccaagtgca agcaagaaaa tacaaaaaaa cattggtatg atttatatat atataaataa
     1261 cataaaaaaa ggaatggctt atcccgatac aacaagcatt cctttaaaaa agattctttt
     1321 atataagtga aaaaagcttt tttccaccaa ttaaataaat ctctcatctc aatagtttat
     1381 ccattatttt atcttttttt gcgtatacct actaaatatt accgtcttct tctcgttctt
     1441 ctactttgtt cgagagaatc tgtactagaa aaagtagttg tatgtctacc ttttctgcct
     1501 atccgagact ttcttttcat atatgggata taaatgatta gatacagtac agcaaaaaat
     1561 aaagcaaata cgcaagcttc ccaactacct aacgccaatt caaccatcaa ggtagcaacc
     1621 ccaacggatg ctgcatacca gataaaaatc agaaacgttt ctttttttct gaccatgcga
     1681 aacatgataa atagactaat aagcaatagt ggcaacacaa atatccaatt gcttccttcc
     1741 atcagccact tgagtatatc aagcagcgac tgtgcaacag cgactaattt tcccatagtg
     1801 aacacacacc tttttttata attaaaaaat tcgccttttt attctactat gaatcttctg
     1861 gcttggcttt catttctctt atgtaatcat tttcttttat acaacttaaa aaaaaattaa
     1921 accattagca taatattttc tgaatctatt cacgaaagtc tttttctatc tttcgttttc
     1981 cacgactcaa ctgatctaga gtcaaaaaac taaaacgaca gttgcgcaaa tgacgcacaa
     2041 gctgccgctt taataacaaa tagcctttca ttctcagcag gccacttatt tccagttaac
     2101 tttctgttct tagcttttta ctcaactgga taatatatgc acttaatgaa gtcccaattt
     2161 ccgaatgttg aagtccatat tcaatatttg tcttcatata tcctaactta tcgcctacgt
     2221 cgtatctttt tcctttaaac tctctcgcat agaccgcctc tgtctggtta agagtctgaa
     2281 gtgcatccgt tagctgtatc tcgtttcctg ctcctggttc ttgagtttct aatatctcaa
     2341 atattttagg agtcagtaga taacgtccga taatggctaa attacttggt gcttttccca
     2401 tttcaggttt ttctacaaac ccttttactc tgcaacgacc cttttcgcta tctgttttag
     2461 gatcaataat tccgtattta gatgtttcat tttctggtac acgcatgacg gcaacagcgg
     2521 aagcttgtgt ttcttcataa tccatcatca attgttgagt caatgggata tcatctttca
     2581 tcaaatcatc ccccagcatg acgacaaacg gttcatttcc tataaatgct tttgcttgaa
     2641 gcacggcatc ccccagtcct cttggatgag attgacggat gaagtgaaga ttgacttgag
     2701 tcgtttcttc aactaaagcg agtaattctg ttttttcctt ttcccgcaaa ttgttttcca
     2761 attcgatatt tgcatcaaag tgatcttcaa tgggtctttt ttcttttccc gttacgatca
     2821 agatgtcttc gattcccgaa tccaatgctt cttccacaat aaactggatc gttggtttat
     2881 ccacgattgg aagcatttct tttgccatgg cttttgttgc cggcaaaaaa cgtgttccta
     2941 gcccagcggc aggaatgact gcttttttga ccttcacttg ct
//
LOCUS       DAYYCY010000217         2904 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.222, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000217 DAYYCY010000000
VERSION     DAYYCY010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2904
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.222"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..364
                     /locus_tag="ACHAZB_002619"
     CDS             <1..364
                     /locus_tag="ACHAZB_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8615235.1"
                     /translation="GYMGLGIFNTVIWANITDVIDDQEVKSGQREDGTIYAVYSFARK
                     IGQALAGGVGGWTLSIIGYDSLAKVQSGEVLQKLYNSSTLIPAVCFFVVGLILFFVYP
                     LSKEKVLENYNLLNQKHN"
     gene            375..1169
                     /locus_tag="ACHAZB_002620"
     CDS             375..1169
                     /locus_tag="ACHAZB_002620"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8615236.1"
                     /translation="MNSWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVPSINRLKHWVSVTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNRQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAIQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDILFKCLSGEYIDIEQKYIDTFWKNLTNK"
     gene            1182..>2904
                     /locus_tag="ACHAZB_002621"
     CDS             1182..>2904
                     /locus_tag="ACHAZB_002621"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="HFM8615237.1"
                     /translation="MTIKVDLRKKPYHLDEDAISWIEETIDAMTLEEKIGQLFVNMGS
                     SRDKNYLSSMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQSPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLSTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNLDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYYKG
                     IISEERLRDALRRILGLKAKLGLHKKEKNKILLPKQEAMEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKDLTEKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGI"
BASE COUNT         1062 a          349 c          580 g          913 t
ORIGIN      
        1 tggatatatg gggttaggta tttttaatac agtcatttgg gcgaatatta ctgatgtaat
       61 cgatgatcaa gaagtgaaat ctggccaaag agaagatgga actatttatg cagtatattc
      121 ttttgcccga aaaattggac aagctttagc tggcggtgtc ggaggttgga ctctttctat
      181 aatagggtat gattcgttag caaaagtaca aagtggagaa gttttacaaa agctgtataa
      241 ctcttcaacg ttgattccag cagtttgttt ctttgttgta ggattgattt tatttttcgt
      301 atatccatta tctaaagaaa aagtattaga aaattataat ttgctaaatc aaaaacataa
      361 ctaggagaaa aaacatgaac agttggatat tttgtattga ttcagatggt tgtgtcatgg
      421 atacaatgac ttataaacac aatctctttt tcgggccaat tgctgcagag atttttgatg
      481 ttcatcatag ggaaaaattc ctacaggatt ggaataatat taatttatat tcagagactc
      541 gtggaataaa cagattttta ggtttagtta aaacattaga aaaaaatgat gtaccatcga
      601 ttaatcgatt aaagcactgg gtatctgtaa caaaagaatt gtctaatcca tctttgaaga
      661 gggaaataga atactctcag tcagaggatt tattaaaagc tttaaaatgg tcggaagaag
      721 tcaatcgaca gattagtcaa gctaaaggat tagataaacc ctttttaagt gctagagata
      781 ctataaaagc tctgaaaaaa tatgggaaga taattattgt aagctcagct aataaggaag
      841 ctattcaaga agaatgggaa agacatgaat tattatcctt agttgatgaa ctgtgttgtc
      901 aagataaagg aaaaaaagaa gacattattc gaagtgtaat tgaaaatggt tgtgatttgg
      961 ataagatatt aatgattggt gattctcctg gtgatctaga agctgcaaat aagaataaag
     1021 ttttctttta tcctattttg gttaataaag aaaaagaatc atgggaaaac ttaagaactg
     1081 atattttatt taaatgttta tcgggagagt atatagatat tgaacaaaaa tatatagata
     1141 ctttttggaa aaatcttact aataagtaga ggagaaagaa aatgactata aaagtagact
     1201 tacgaaaaaa accatatcat ttagatgaag atgctatttc ttggattgaa gagacgattg
     1261 atgctatgac tttagaagaa aaaataggtc aattatttgt taatatggga tctagtcgtg
     1321 ataaaaatta cttaagtagt atggtcaatc agtaccatat tggagctgta cgatataatc
     1381 caggaactgc tgatgaggta tatgatcaaa actatatcct acaaacagaa agcaaaattc
     1441 ctttattaat tgctgctaat acagaagcag gtggtaacgg agcttgtaca gatggtaccg
     1501 aagttggtgt agaagtaaaa attggtgcaa caaatgatgc gaaatatgct tatgaattag
     1561 ggagagtttc tggaattgaa gcatcggcta ttggatgtaa ctggagtttt gctcctattg
     1621 tagatattaa tagaaattgg agaaatccta tcatttctag tcgatctttt ggacaaagcc
     1681 ctgataaagt tttagaactt agtttagctt atatgaaagg tattcaagag agtggaattt
     1741 tgcctgcagc taaacatttc cctggtgacg gtattgacga acgagatcaa catctatctt
     1801 ttagtattaa tagtttaagt acagaagaat gggataattc ttttggaaaa gtatactcag
     1861 gcttaataga agctggatta ccttcaatta tggcaggtca tatccatatg ccagcttatt
     1921 caaaacattt taatcttgat ctgaaagatg aagatttact tcctgcaact ttatcaaaag
     1981 aattaatcac tgacttactt agaggaaaac tcgggtttaa tggattagta gttactgatg
     2041 ctagtcatat gttaggaatg actggggcaa tgaaacggtc agaattattg cccacttcta
     2101 ttgcggctgg atgtgatcta ttcctatttt ttaatgatcc agatgaagac tttaattata
     2161 tgatggaagg ttattacaaa ggaattattt cagaagagcg tttacgtgat gcattaagaa
     2221 gaattttagg cttaaaggct aaactaggtt tgcataaaaa agaaaagaat aaaattttgt
     2281 taccgaaaca agaagctatg gaaaaaattg gtttaattga aaataaatca atttttaaag
     2341 aggtagctga tcaagcaatt acactagtta aagataaaca agatatttgg cctattgtac
     2401 cagaaaaata taaaaggatt ttactggtag atgtacatgg ggtaaaaggt gggtttggtg
     2461 aagtagttgc tggaaataga cctcaggcca ttgatttatt gaaacagatt ttgtcagcaa
     2521 aaggatttga agttgaaaaa tacatttcac ctgaagaaga attgaaagga ttatcgcaag
     2581 aagaaaaaat taaaaatata gctggaatat atgctgctaa gagaccaatc aaagatttaa
     2641 ctgaaaaata tgacttgatt attaatatag ctaatgttaa cccaggtaca gttcaaagaa
     2701 ttgtttggcc agcaagtaaa ggtactccgg atattccatt ttatattcat gaaattccaa
     2761 caatttttgt atctgtacag tgtccgtttc atttagctga tgtgcctcaa gtaaaaactt
     2821 atatcaatac ttatgatgga aaacaagaaa ctttagaaat attagtggac aaactaatgg
     2881 gcctttctga atttaaggga ataa
//
LOCUS       DAYYCY010000218         2863 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.223, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000218 DAYYCY010000000
VERSION     DAYYCY010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.223"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            175..519
                     /locus_tag="ACHAZB_002622"
     CDS             175..519
                     /locus_tag="ACHAZB_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFM8615238.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            520..939
                     /locus_tag="ACHAZB_002623"
     CDS             520..939
                     /locus_tag="ACHAZB_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFM8615239.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVIFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1031..1381)
                     /locus_tag="ACHAZB_002624"
     CDS             complement(1031..1381)
                     /locus_tag="ACHAZB_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8615240.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1347..2677)
                     /locus_tag="ACHAZB_002625"
                     /pseudo
     CDS             complement(1347..2677)
                     /locus_tag="ACHAZB_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
BASE COUNT         1075 a          504 c          544 g          740 t
ORIGIN      
        1 aaaataaaaa aagcgttagc aactatgctt aaaaagcata tcgactgaat aaactaagca
       61 ttggacaggc tgaaagacta catttaaaat gaaatgcgaa gaaacaatag aaaataaagt
      121 tgccttagaa ccatagttga atcacctaac agtcgtgaag ggagaataag agaaatggca
      181 gaaaaacaaa ccgcaggacg agatcaatta ggagattttg caccaaaatt tgcagaattg
      241 aatgatgatg ttttatttgg acaagtatgg tccagagaag aattatctgc tcatcagcgt
      301 agtatcatca ccatatctgc attaatttca ggaggaaatt ttgaacaatt gccagctcat
      361 ctgaagattg gcaagcaaaa tggcattact agtgaagaaa tagcagaaat cattacccat
      421 ttagcttttt atgtaggatg gccaaaagct tggtcagctt ttagtttagc aaaagaaatc
      481 tatttagaag aaaatagcga agataagaaa gaggcgtaaa tgacgacaaa acacgaagaa
      541 atacacaatg gcgtgatttt tccagcaggt gaaaaaaacg aagcttacgc tcaagtattt
      601 accgggcaaa gctatctgaa tatgttagtg aatgatcccg aagtttctgt tggtgtaggt
      661 aatgtaattt ttgaaccagg ttgtcgcaat cactggcatg tccataaaga cggctatcaa
      721 atcttacttg tgaccggagg agaaggctgg tatcaggaag aaggaaagcc agcaagacga
      781 ttaatggctg gagatgtcgt cgtaacgcac aaagggatta aacattggca tggtgcgaca
      841 aaagacagtt ggtttgaaca tttggcgatc actgctggta attcagagtt tttagaacct
      901 gtaacagatg aagtgtatga aggattggaa aatcaataat gtcaagcttg aacagctcct
      961 gaaagtagat tttaatcgaa ctttcaggag ctgctttttt gattcagtct tttataaata
     1021 gaaaataggg ttaataatag gaatgagaag cacgtcgaaa gatcttaata ttgataatcg
     1081 gctgggcata catgatttcc acgtcatcaa tcaaactacc taacagatga taatcttccc
     1141 gatcatgtcg gggattacct aatagttctt gatagtagat ttccatacta gggtcccgag
     1201 gattgttcag caaatcagca agtacatcta aatcttctgg ctgagtttct gccgtaccct
     1261 gtatctctat gaaaagccct tcctcagcaa attctactcc aatacgtatt tcagttattt
     1321 catgggaata taaataagta agaagctcat cgatgattct ttttgataat tctttttcca
     1381 agtgtctttt tttcctttct gacagcggta aagttatcta gtatcggtac aaggatacct
     1441 gcaacgaaac cagttgaaaa accactgtta tatagattta gtcctccgtg caaagtgctg
     1501 acgttatgga caagagcaat atgtagtaca ccagctatta caccgtaaaa actgccgtaa
     1561 aaaccgctta tgggcgcgag acctgtccca aacacagctg caacaagcat gccagtactg
     1621 gtgatctcat gaaaagttcc aaacaaagaa gcaataaata ttccgactag tatcggaaaa
     1681 ctgtttttta aatgacaacc aaaagcactg aaacccaccg cagataaaat agcaccgatg
     1741 acaggtccat ttaactgacc gccaaccagc aaaacataag aagacagcat cagaccaact
     1801 aagcctatgt tgatcaaagt agcaccaata ccagaattag caatgaaatc agttgtcaat
     1861 tttccagatg aatcgaaaat ttctcgaatc cccaaaagcc gaaaggaatt ataatagaaa
     1921 ccaattccga ctgtcagcga aaaaaagcca aaaataatcc aaatgagagg aaagtgatat
     1981 tcattgaaaa ccaatgtatt ttcgacgatt gaatagctga ataacctcaa aacagcagta
     2041 aaagtcatcg caatcaaccc agaggtgaat ccaatattat aaagtgtgaa tccacgatgg
     2101 aaaactaaag tttgagcagc taagggcgga agaagtagac caattgctat gccagctaaa
     2161 ttccccaata aaatacctat cagtaaagga aaacgcatac caaaagtgat ataactaacg
     2221 accggtgata atgcgctacc aaacagtccg atcatgatgt actgagaaaa aggtttattg
     2281 acaaatacag cgtatagata cacacctaaa atgattgaga tagaattata aaggtttttg
     2341 ccaaaaaaag agaaaccggt aactgttaga atcgaagcaa ataagggacc gtttaaaatc
     2401 accttgtgcc gataagctaa aagcacagaa agtaaggtca aaatccctac atttatgaat
     2461 gcgcttccga aaccgcctaa agcaaaataa tctgtcaaaa gattgcttgg cgaggtaaga
     2521 atagttagca atccactcca attatcagta ggagtattag aaagcaagcc aataaaaaga
     2581 aaaaaacacc tgtaaaacca agataaaaat actttctttt ctgtgaaacg attcgttttt
     2641 gaggatgaat ataaataacg tcagtttcgt tttccattac acaccttctt tttcattttc
     2701 tttaactata acttgaaatg aaagatttgg acaactatca tttaggtcaa aactaaaaat
     2761 gtaagggaac ccattgaatt taaccaaatt tttttaattt tgcaaaaaaa agttaaagaa
     2821 gactgaaaaa aaatttaaaa tataacgttt tgagtaaaat gtt
//
LOCUS       DAYYCY010000219         2826 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000219 DAYYCY010000000
VERSION     DAYYCY010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2826
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.224"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            798..1052
                     /locus_tag="ACHAZB_002626"
                     /pseudo
     CDS             798..1052
                     /locus_tag="ACHAZB_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405927.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
     gene            1122..1634
                     /locus_tag="ACHAZB_002627"
     CDS             1122..1634
                     /locus_tag="ACHAZB_002627"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8615241.1"
                     /translation="MTNKNNERYSTELKATAIKKMMPPENRGIRQLSQTLGIPEATLY
                     SWRKKARIQGNTTPGTKKNVEKWSSEDKFLIVMESFSMNQVELAEYCRKKGLFKEQIE
                     AWKKTCLSANEQEENRTRELATELKEEKKQARQLEKELRHKEKALAEAAALLLLRKKA
                     HAIWGDQEEE"
     gene            1631..2668
                     /locus_tag="ACHAZB_002628"
     CDS             1631..2668
                     /locus_tag="ACHAZB_002628"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM8615242.1"
                     /translation="MISSSDRKQAVELIQEANRNGARLTYACNELNISVRTYERWTRE
                     GTIAHDQRPLAKRPVPKNKLTDQEREKIIETVSKKEFMNLPPSQIVPKLADCSIYIAS
                     ESSFYRVLREKNMQHHRGRSQVSQKRIPPSHLATKPNEVWTWDITWLKGPIKGLFYRL
                     YLIIDLFSRKMVGWEIWESEEARHAEKLIKQTVLKERIQGQPLVLHSDNGSPMKAATF
                     QVLLEKLGIQSSYSRPRVSNDNPYSEAAFRTLKYRPEYPNNGFKTIDDARKWTKEFVD
                     WYNDSHLHSGIQFVSPAACHEGRHLAILEKRKQVYEDAKKRHPERWSKGVRNWTPHQS
                     VALNPLKETES"
BASE COUNT         1022 a          496 c          564 g          744 t
ORIGIN      
        1 ttgatgacca cgaaagtgca tggcgatatt tgatgttgat actttatgca gtaccctatg
       61 tattcttcgc tatcattctg tggctcatag cattgttttt tagagtattt aatgaccttt
      121 aggtagattt atatataaaa agggctaaat acgcaaaata cccccttaaa atgcaaaata
      181 aatgcaaata agggggtaac tccttatgaa aaaacaacta atagtattat aacacgtttt
      241 tatgccgaga aaacttattg gttggaatgg gctatgtgtt agctaacttg ttagcgagtt
      301 ggttggactt gaattgggat taatcccaag aaagtaccaa ctcaacaaca cataaagccc
      361 tgtaggttcc gaccaataag gaaattggaa taaagcaacg aaaggagttg agtaaaatga
      421 gtgagaaaac gaagaaaatc attcgagtat cattagcttt ttcacaagta gtagtgcaat
      481 cattgatagt ttattatact taccagcaat ataaattatc taaagagaat taagttttga
      541 ttatgtaata agttttggta ctaaaaaaga cttgatctaa ttagaccaag tcttttgtta
      601 gtgttatatt aataacaaaa taaaaataag aagtcgctca ctccccgacc aaagtttgtg
      661 agcaaaataa acagtcgatg tttgtttctt aattgtatat cttgatatta aacgatttaa
      721 gattattctt caagatatat attcgcaaat gtaattggaa aagcgacttc ttaaacaaaa
      781 ttaaggagtc gctttttatg gcaaaaataa cgaaaaatga gcaagaaaat ttggtggaaa
      841 attacacaaa aaaaccggcg tactctaata gccggttaga cgcacatttc gtgtgcactt
      901 ctaatccgaa attgagcttt gacgctatga cgatagtcgg aaaccttaat aaaaacagcg
      961 ctaaaaagct atccgatttt atgagtttag atccacaaat tagactttgg gatatacttc
     1021 aaacaaaatt taaagctaaa gcactacaag accccctatg ttaggctagt tgtcgtatag
     1081 gataataagt tataataaag actatacgga ggatgattac catgacaaat aaaaacaatg
     1141 aacgttactc taccgaactg aaagcgacag ccatcaagaa aatgatgcca cccgaaaata
     1201 gggggattcg ccaattaagt caaactttgg gcattccaga agcgactctt tattcttggc
     1261 gtaaaaaagc tagaattcaa gggaatacta cacccggaac gaaaaaaaat gtcgaaaaat
     1321 ggtcaagtga agataaattt ttaattgtga tggaatcatt ctcaatgaac caagtagaac
     1381 ttgcggaata ttgccgaaag aagggcttat ttaaagaaca aattgaggct tggaagaaaa
     1441 cgtgcttatc tgcgaatgaa caggaagaaa atcgaacgag agaacttgca actgaactaa
     1501 aagaagagaa aaaacaagcg cgtcaattag agaaagaatt acgtcataag gaaaaagcat
     1561 tagcggaagc cgctgctctc ttattactaa gaaaaaaagc acacgcgatt tggggggacc
     1621 aagaggaaga atgatttcat cttcagatcg caaacaagct gttgaactta ttcaagaagc
     1681 caatcgcaat ggggcccgtc tcacctatgc ctgtaatgag ctgaatatta gcgttcgaac
     1741 ctacgaacga tggacgcgtg aaggtacgat agcgcatgat caacgccctt tggcgaaacg
     1801 tcctgtaccc aaaaataaac tgacggatca agaacgcgaa aaaataatag aaaccgtttc
     1861 aaaaaaagaa tttatgaatt tgccacctag ccaaattgta ccaaaacttg cggattgctc
     1921 gatttatatt gcatcagagt ctagcttcta tcgtgtattg cgagaaaaaa atatgcaaca
     1981 tcaccgtggc cgaagtcaag tttcacaaaa acgcataccg ccaagtcacc ttgcgacgaa
     2041 gccaaatgaa gtttggactt gggatattac ttggctaaaa ggtccaataa aaggactctt
     2101 ttaccggtta tatctcatca ttgatttatt tagccgtaaa atggtaggct gggagatttg
     2161 ggaatcagaa gaagctcgtc atgccgaaaa acttattaaa cagacggtgc ttaaggaacg
     2221 tatccaagga cagccgttgg ttttacactc cgataatggc agccctatga aagccgcgac
     2281 ttttcaagtc ttactcgaaa aattaggaat tcaaagttct tattcaagac cacgagtgag
     2341 taatgacaat ccttattcgg aagcggcttt ccgaacgtta aaatatcgac cagaatatcc
     2401 caataacggc tttaaaacaa tagatgatgc tcgaaaatgg acgaaagagt ttgtcgattg
     2461 gtataacgat tctcatttgc atagtgggat tcaatttgtg tctcctgcag cgtgtcatga
     2521 aggacgacat ctcgctattt tagagaaacg aaagcaggtt tacgaggacg ctaaaaagcg
     2581 ccatcctgaa cgctggtcaa aaggtgttcg aaattggact ccacatcaat cggtggcgtt
     2641 gaatccttta aaagaaacag aaagttaaca ggagcataat ctttcaagat tatcaaataa
     2701 ccctcgtcac ccaatagggg aaaaagttat tcttccccta ttggataacg aggccaaccg
     2761 accataggga ggttctgctt tttgataggt ggaaaataaa aatacgacaa ctatattgac
     2821 aaacac
//
LOCUS       DAYYCY010000220         2774 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.225, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000220 DAYYCY010000000
VERSION     DAYYCY010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2774
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.225"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            190..690
                     /locus_tag="ACHAZB_002629"
     CDS             190..690
                     /locus_tag="ACHAZB_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFM8615243.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            787..2634
                     /locus_tag="ACHAZB_002630"
     CDS             787..2634
                     /locus_tag="ACHAZB_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFM8615244.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
BASE COUNT          974 a          354 c          601 g          845 t
ORIGIN      
        1 caatctttag tcttactagc gattgtggtc attggtttgt ttttaggatt taaacgcgag
       61 ttttccaaac tgattggctt cttggtgatt gctttgattg cggtaggtct ggtctttaat
      121 gcttctggtg tgaaggatgt cttactcaat ttgtttaacc gaattgtcgg tgcctgaggg
      181 ggaatcacaa tggaacagat acgtgtttat attgcgaacc ttggaaaata caatgaaggt
      241 gagttggtgg gggcatggtt taccccaccc gtcgattttg atgaagtgaa agaacggatt
      301 ggattaaatg acgaatatga agagtacgca attcatgatt atgaactgcc ttttgagatt
      361 gatgagtata caccaattga ggaaattaat cggttgtgtg gtttagcgga agagcttaaa
      421 ggaacgccaa ttggtgaggt cgcttcagag attcaacatg ccttcttcaa ttctttcgag
      481 gaaatggtag agcatgtgga tgatatcatc tattatcccg attgtaatga catgagtgat
      541 gtagcgtatt acttgattgt agaagctggt gatttaggcg aagtaccaga acacttgaag
      601 acgtatattg attttgatgc gtgtggtcga gacttggaga ttggcgggaa ttatttggtt
      661 acgagtcgag gggtttttga actaatgtaa atcatttgtt agaattgacc agaaatttct
      721 atcgtacttt tacttttcta tttgatatac tgaaatagaa aatatttgtt gataggggtg
      781 ttactaatgg ttggcaataa gtggtacaaa tgtgatcttc atattcattc aactgcgagt
      841 cagtgctttc aggataaaaa ggttactgct aaggaatggg tagaagaatg tttaaacaaa
      901 ggactcgatt gtgttgcatt aactgatcac aatacagggg aaaatattga tgaatataag
      961 aaagtggcta tcgaaaatgg cttagatttt tttcctggag tggaattaac gtgtggagaa
     1021 aatggaactc atttactagt cctatttgat aaaagtgcat gtacaaatga tatctctgat
     1081 ttcttaacca agatgggtat aaaaagagag gattgggcca gcgctgcagc ttgctcaatg
     1141 aaatcagttt tagaagttat tgattttgct gagcacgagg gcttgctcgt tattcctgca
     1201 catatagatg agtttaatgg aatttcttca gcaaaatttg gaaatctagg aaagatattt
     1261 gaatcagaaa atatatatgc agtacagacg gttcaaaaag aattttttga aaatcgctca
     1321 caaatgattc cctcatctaa gcgtgacact atctatgata gtgtgaatgc cagatatgat
     1381 ggtagagttg gaaaagatac tttggaatct tggtataaat cagttgttga ggcagaaaaa
     1441 aataatatga catttcttag cttttctgat aacccacact cgaaaggaaa ttcaaaacat
     1501 ggactttggg gaataggcac aagatattcc tatataaaaa tgaaggacga acctgacttg
     1561 ggatcacttc gcgatgcttt aatgctaggc gaaactcgag ttcattctga tttttctaat
     1621 ttctccatta atgaaaatga agttttattg gaaaaattaa gcttttctgg gaccactctt
     1681 agcacaaaag aagttgtagt cgaattcagc gataatttaa cttcaattat tggaggaaga
     1741 ggaacaggca aatcttgtat tacaagattc ttggtctatg tccttggaaa ggaagcagaa
     1801 cttgatcaat tttctgaaat acaaagtgat tatcagaact ttgcacaaat tgaacataat
     1861 ggctctggaa tatttttgaa ggatacaatt gtgaaactga atattttcta taaggggaca
     1921 aaatatcaaa taataagaac acaggataga cattctattt atgaatttac acaagagaat
     1981 ggtctggttg aaaaagaaac agaacggttg agaatgattt ccgataaggt gagtatatac
     2041 tctcaaaaac aaatttatga aatttcaaaa aatcaaacta gtatactaga attactagat
     2101 ggatataact ctgatttgat ttcagagtat aaaaatgaga ttgaaacatg tgtaaacgaa
     2161 ataagaaaat taaattggga tattgtttct gtgaaaaaag aaatacaaga taaggcgaag
     2221 gtagagcttg aaattgaaga tttaaaaaaa caagtagaaa agctatctca taagagttat
     2281 aaagatgttt atgatgagta ttcaaaagag acagatattt atagggaact gaaacgtgat
     2341 gtggaggcac ttaaggaaat tcccaaaaat ctaactgata gtgttgacaa aattgacttt
     2401 ggcaatggta taaaagttac agacgaaatt gatgaacacc gcggagaatt gattaagaat
     2461 ttagtgacca atcgggctaa gattcaaaca ataattaatg aaatgtcaga tgaaattgat
     2521 agttatagag cagagctgaa taaatcagat tggaaagtta actataatga ggtttcaaag
     2581 aggtatcaaa gaggtatcaa aaacttagtg aagagttatc tcaagatgaa ttaaaaatgt
     2641 tacaaaacct tgattcttta tatcaatctt tatcaaaaac tgaagagcga ttggaagaaa
     2701 ttattcaaca agaaataaag cttaaagatt accaagttga ggttgactca gcaatgtcaa
     2761 gaatgaataa atca
//
LOCUS       DAYYCY010000221         2722 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.226, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000221 DAYYCY010000000
VERSION     DAYYCY010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2722
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.226"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(76..1170)
                     /locus_tag="ACHAZB_002631"
     CDS             complement(76..1170)
                     /locus_tag="ACHAZB_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HFM8615245.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            1359..2120
                     /locus_tag="ACHAZB_002632"
     CDS             1359..2120
                     /locus_tag="ACHAZB_002632"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM8615246.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(2235..2675)
                     /gene="fabZ"
                     /locus_tag="ACHAZB_002633"
     CDS             complement(2235..2675)
                     /gene="fabZ"
                     /locus_tag="ACHAZB_002633"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFM8615247.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
BASE COUNT          859 a          546 c          464 g          853 t
ORIGIN      
        1 cctataaaaa aacaaaataa aaaagcagaa ataactggct atttctagcg gattatttct
       61 gcaataacta attatctatt ttttattttc aattaattta aagcttgacc atggtttcaa
      121 aaagtcaagg aggaataatt cgtcatcgga gattcttcct acaacattga cacgtccgtc
      181 gtttttcata tccttcaagg cgatttgtgt ttctcctttg tattgaccgt acccttcatt
      241 gtcaatcagt acatccccat gatgcatatc tcgcgtatta tgaggcggga aatctttatc
      301 tttgtagtat acacgagtca tcgttgaacg aaggatatat tctgagcgat cccctcgata
      361 actatgaagg ttatcaaaca agcagattcg ttcgttttct gtaatacctt cttccgtatc
      421 tactttcaat gttggataat ccgcgttaaa tgcttctgcc atttctttca attcttcttc
      481 acttgcgtac gcatttccaa tagaaatatc atcaatcaat cctgtaagga ttaaatgctt
      541 cacttgtgtc gcaatttcaa gatctcggtg gtcttccagc gtgcacaagc catcttgcgt
      601 aggccaaggg ccaaactcag cagattgcga attgacgaat gccatcgtat tcaaattata
      661 ttttctgaat ttttctgaac aaaaaacaaa gtgttcgtat cctaaaccag agtatcgatg
      721 aggataaaaa ttatgagaac ctaataaatt atccgtgttt ggcgaataac tcatgatgtt
      781 gtcaacgtaa ttcgtgcccg aactcatatt gatctcgatt ttgataccaa aaggattgcg
      841 cgtcattttt gcttcttctg ctcctgtgaa tccgatatct aagcggaccc cgtaggcacc
      901 catcttatgg aaaaaagata agtcatcgta agaaatctcc aactgttcaa ataacgctgg
      961 attgatatca accattactt ccatgcctag gctattcgcg taatcaacta cttctttaaa
     1021 ttctgaaaga actttttctt tatcatcatt gatctgcagc aaactggtaa aaacccgttt
     1081 atacccatac ttatgagcta aatccaagta cgctttgtcc ttttcaaatg ttgaccgttc
     1141 tggataaatc gagattccta attttcccat attaccattt cctcctgcat cttttcgttt
     1201 ttgtatccac ttacattata gcaaacacaa agtaaatggt ctacaccttt tctttttctt
     1261 ttaaaataaa tgataaattc caataattta agtaggtatt ttccctctat tttgttatca
     1321 ttaaagataa caaaatagta aattggaaag gaacagttat gaagaaaaat aaagttattt
     1381 taagtattag tttagcagca gcattgctgt ttgctgctgg ttgttctgca aacaacacga
     1441 cttcttctag ttcgtctaca gaaagttcaa aaagtacatc tactgcggaa agcacgactg
     1501 ccagtagcgt cgatttgaac gcattggaat taccacaatt gacgaaagat gtcgccgaaa
     1561 atgaagatct ggtcgaaatg gtgacgaata aaggcacaat caaaatcaaa ttatttcccg
     1621 aacacgcacc aaaagcggtt gaaaacttta tgacacatgc aaaagacggt tactacaacg
     1681 gactgacttt ccatcgcgtc atcaaagatt tcatgatcca aggtggcgat cctaaaggcg
     1741 atggcactgg aggggaaagc atttggggag aaggatttga aacagagatc aacaaccacc
     1801 tttataatat aagaggtgcg ttatctatgg ctcgctcaca agatcctaac agtaacggca
     1861 gccagttctt tatcgttcaa aatacagatg atatgcatga tggtctttta aaagacgact
     1921 atcctcaagc aatcatcgac gcttataaaa acggcggtta tccttcacta gatggccatt
     1981 atactgtctt cggccaagtg attgaaggaa tggatgtcgt ggatgctatt gcaaatatcg
     2041 aaacagatag ttcagacaaa ccaaaagaag aagtaaaaat cgataaaatc aatatactgc
     2101 aagaagcaaa acaaaaataa aaaaataacg tctgaatcct gcatagatga caacgtatgc
     2161 taggattcag gcgtcttctt tttacataaa aagcaagtaa gcttcctttt gttttgctat
     2221 atcaggtttt atagtcaagc attttggttg acgatgaacg tgaactcgca ctcacagact
     2281 tttttatcat ctacatatgc tgcagataac gctgtaccga ctgatccctt cattttggtg
     2341 atttcaaaat gacatttcag cacatctcct ggaacaactt tttgacggaa tttggcctta
     2401 ttgatcccac cgatataagc tctttttcct agaaactctt ctgattttag gataagtatc
     2461 gaacctgctt gtgccagtgt ttctagaatc aacgcacccg gcatcactgg attgtcagga
     2521 aagtgacctt ggaaaaaatc ttcattgatt gttacatttt ttgtcgcaac gatctttttt
     2581 cctggttcta aagtatctac ataatcaata taacaaatag gataacgatt aggaatcaat
     2641 tccattactt cagtagcaga taatacgtaa gacataatta aactccttct ttcaggcaat
     2701 tgccattttc attactttga ct
//
LOCUS       DAYYCY010000222         2688 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.227, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000222 DAYYCY010000000
VERSION     DAYYCY010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2688
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.227"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            75..545
                     /locus_tag="ACHAZB_002634"
     CDS             75..545
                     /locus_tag="ACHAZB_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFM8615248.1"
                     /translation="MGDSEETHIQHTFDSFCKKVVCNEALNIQKKYARFRQRQISMEI
                     LEQKGLGTEFYYLEPNLNSSGTFIVLGMKIFISNEELANAIAALPSQRQEIIFMSFFI
                     GLNDREIGDIIGKSLGSVWYQRQMALEELGFPYFDFGWHIFHAKFCDFNSIGKI"
     gene            complement(465..683)
                     /locus_tag="ACHAZB_002635"
     CDS             complement(465..683)
                     /locus_tag="ACHAZB_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8615249.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWKS"
     gene            790..1104
                     /locus_tag="ACHAZB_002636"
     CDS             790..1104
                     /locus_tag="ACHAZB_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8615250.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            1208..2386
                     /locus_tag="ACHAZB_002637"
     CDS             1208..2386
                     /locus_tag="ACHAZB_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8615251.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
BASE COUNT          883 a          391 c          547 g          867 t
ORIGIN      
        1 tcctagattg tagtaataat ggaaagggat agaagtaaaa cgagttgaat gaattttttg
       61 agggggagaa tattatgggc gactcagagg aaacccacat tcaacatacg tttgacagtt
      121 tttgtaagaa agtagtctgc aatgaggcgt taaatattca aaagaagtac gctagatttc
      181 gccaacggca aatatcaatg gaaatattgg aacaaaaagg gttaggtaca gagttctatt
      241 atttggaacc aaatttgaat agtagtggta cttttattgt ccttggtatg aaaatcttta
      301 tttccaatga ggagttggca aatgcgattg cagcattacc tagtcaaaga caagaaatca
      361 tttttatgtc cttcttcatc ggtcttaatg atcgtgaaat tggcgatatt attgggaaaa
      421 gtctcggcag tgtttggtac caacgacaaa tggctttgga agagctagga tttccatatt
      481 tcgattttgg ttggcatatt tttcatgcca aattctgtga ttttaatagc atcggcaaga
      541 tttaattttt cttcagctct ctctccactt ttaagttttt ttactgtact caagttgatt
      601 cctgttccga atgctattgc attagccgta gatttttcaa gaatttcttt aattattttt
      661 atgtctgcaa ttccttgttt catattaaaa attcctcttt tctagtccgt tttgatggag
      721 tactggagtg tttttttaca attttaaggg gtaccgtggt ggtcgttttg attttgagag
      781 agaagggtga tggagttgga agttaaaaca aatgttaatt ctctggaaat agagttctat
      841 tcaattttac aaaaaaaact ggaagattac acggaaaaat tgcttgcagt cagtcattta
      901 cctctggtat taacaaatac tgatttaaag cgactatttc agataagtga tagtacactg
      961 aaccgtttag taaaacttgg tgagtttcct ccctgttggt acgggattcg aggacactat
     1021 tttcgtgatg atgttttaga atggatgaga aagagtgatt cgaatgacta tcgagaaaaa
     1081 ttgcgactac ttcgatcttt ataagttaca ttttctttct aagatgcggt acaatgatga
     1141 catagataga aagatttcca aagggtttct gcttgttggg ttccttcaaa ataggaggaa
     1201 tcaataaatg gaaaaaacag atcgattcaa gaagcaaatc agttctactg gtaggatcac
     1261 atggtacttt caagcatatc gaacaacaag acgtggcttt aattcaaaac gtgaggcgca
     1321 actcgcttat ctggaaatgg agaaacaaca aaggcttaag aaaaaaattg ttgcaagcaa
     1381 tgacaagttt agtgttgtag gtgagcaatg gttccaatat taccgttcgt taaatgaaca
     1441 aaaggaaata acctatgata agcgagagga acaattaaaa attattgttc gatggattgg
     1501 tgataaaaag ttgtctgatt tgacaccaga gtgccttcaa gaattgcttt ttaccttaaa
     1561 ggaatgtggc gttaatggaa caaatgttgg ctatgccaaa aatagtttac aatcacttat
     1621 tcaagttcta aatatgattt ttaaatattg tcaaagaaag aatttgataa gtgaaaatcc
     1681 aatgaagtct gtgagaatgc caaaatatca gttaacggta actgatttaa aagaagccat
     1741 aaataatatt gaggacaagt ttcttactgt cgatgaattg agaacttttt tgaattatgg
     1801 cattgttcat gaggaattac cgatgtctgt acttttccat gttctatttt acactggctg
     1861 ccgagtcagc gaggcgcttg ctctacagcc tggagatata gatttcaaaa gaaatgaaat
     1921 tctgttctat aagcaaacgg cagttaaagg taagagtaag gattttcgaa ttgaaactac
     1981 taagacagtt agttctgcta gacgagttct agtaactcct ctagttatgg aaaagctaca
     2041 acagttgatt aaggctttgg gtgagatgag gcaacattac aggtttatta tagaagaaac
     2101 atatttgttt gtgtatcttg attctagtaa acgtggtgtt ccttatcgtc gagagtatgt
     2161 aaatgatcac gtcaaacggt gcgtgaatcg ctcaggaatt aataagccat ttcatactca
     2221 tttagctcgt catacaatgg caagcttagt agcggaacac tgtagctggg aagtcttgaa
     2281 agatcgctta ggccatactg accaaacaac ttcgaaaatt tatcgccact taacgtcaaa
     2341 tgaaaaggta aaacctctga ttgcttttag ttctttagag agttaaaaaa acggtgaaag
     2401 cactggtatt acaggattct tgaaaaacga ttttttaaca aattagtcaa tatgtagtca
     2461 gaggtaataa aaatcctttt atatcaatac tttaacgact tttcacaagt atcaaagaaa
     2521 tccgtcgtta atcattgtta tcagactgag aaagaaccct tggtattcaa gggttctttt
     2581 ttcttagcat gtaatttgaa caccctgatc cagttttttc tttaattatt ggtaggggag
     2641 aataatggat tagaataaat ctacctataa gtagtagtaa aaaaaaga
//
LOCUS       DAYYCY010000223         2614 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.228, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000223 DAYYCY010000000
VERSION     DAYYCY010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2614
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.228"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..182)
                     /locus_tag="ACHAZB_002638"
     CDS             complement(<1..182)
                     /locus_tag="ACHAZB_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFM8615252.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
     gene            517..951
                     /locus_tag="ACHAZB_002639"
     CDS             517..951
                     /locus_tag="ACHAZB_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFM8615253.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            974..1294
                     /locus_tag="ACHAZB_002640"
     CDS             974..1294
                     /locus_tag="ACHAZB_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8615254.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1435..1626
                     /locus_tag="ACHAZB_002641"
     CDS             1435..1626
                     /locus_tag="ACHAZB_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8615255.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1697..1954)
                     /locus_tag="ACHAZB_002642"
     CDS             complement(1697..1954)
                     /locus_tag="ACHAZB_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HFM8615256.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            2112..2375
                     /locus_tag="ACHAZB_002643"
     CDS             2112..2375
                     /locus_tag="ACHAZB_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8615257.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            2399..2587
                     /locus_tag="ACHAZB_002644"
     CDS             2399..2587
                     /locus_tag="ACHAZB_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8615258.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
BASE COUNT         1003 a          373 c          537 g          701 t
ORIGIN      
        1 gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt ttgagaaatc
       61 gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc agtcgttccc
      121 atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt aagtgatgtc
      181 atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg aggaatactt
      241 taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca aatccacgtt
      301 ttttgtaaca gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc acataatgca
      361 gaaataatgg gattagaaca ggtgttctga tgagaaaaga aatgtataaa taacttgaaa
      421 gaaattgctt ttgttcatca ttttatttac atattgcgta tattttttgt atttttgctt
      481 gagatgttac aataaaagaa aaaggaggcg gttccaatga gtaaaagata ccagaacatc
      541 atggtagcag ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc ccttgactta
      601 gcaaaggaca atgaagccca cttatttatc gtgtcaatca tcaacaaagt tgagctgacc
      661 catagtgcct atgccttttc aaaaatctat gcagatgaaa aacaaagaac agaagtagcc
      721 atgctgaaaa aaatcaatga tgccaaagag tacggcattg ctgagatcca cgcaatagtt
      781 gaaactggag atccaagaaa tttgattgcc aatgtcatcc ctggacaaga aaatattgat
      841 ttgatcgtta tgggagcgac aggaaaaggt gctatacaac aagcattagt tggatcgacc
      901 gcttcttatg tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta atgagataag
      961 ggaggaatag aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa aaatagaaat
     1021 tttagcaatt attgaagacg gcacacaaac aaagaaggga tactcgatcg ctttagtaaa
     1081 gtggaaagga aaaaaaggtg ttgctctgag atgggatgga gataaccaac aagataaagg
     1141 attccctgtc acagctaacg gttatcatcc agcgtggttt gttctgcctg ataaatttat
     1201 ggaattatac agctatgatt atgcaagagc cgcagcatca atcaaagcat tagataaatt
     1261 aacagaagag cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc agcctttaga
     1321 aaactgatta gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa actagaataa
     1381 tgcgtataac ttaaataaaa cttaaagcaa taaaatggca agagggtgag gcaaatggaa
     1441 gcaggaaaag tattacagga agtaaaagca ctatgtgcag ataaaaaata tgaagaagcg
     1501 aaaagcctga tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat cgcacaagaa
     1561 tttatagaat taaaacaagg aacgatcctt caccgattca aacatttttt tggtataaca
     1621 gaatagaaaa aacgattaaa gatcggcaga aatttgctgc atctttaatc gttttctttt
     1681 taaacatgcg agggattcaa ttctgattct tatataaaaa atagatccaa ataataagaa
     1741 aaattactga aagcgtcaat tcgatgatgt gtgttattaa tacagacgtt gtttcccaat
     1801 gcctaaaaga aaaaatttga taagtaaaat atacagcagg taaagaggcc agtaaaactg
     1861 aaaacttctt ttttgtccat tttatgtaat agaaaagaat gcttatcata ataaaaaaag
     1921 gaatataaaa aacaaattga ccgatcgcga tcatactatt gcctccgata aataaagtcc
     1981 atgatacaaa ataacacttt gtgcgaaatg gcgcaagcag ttttgtggat ttatccggta
     2041 aaatctgtat cgattttgaa attagaggag aaacttgcta taatcaaagt gaaagaagga
     2101 gtgggatttt catggatcat gtagtgttag aaatcgattt gcatttgatc aataatattc
     2161 gggtgattta ctatgttgta agtaacagtg tcgaacaacg tgtactgacg aataaaataa
     2221 atggtatttt agcaaagaaa gacgttcacc gattcaataa tggtgaagga agttattata
     2281 gtatcccagt agaaaaaatt atttatacga cagtcaaagt cagagaagat ctagatacca
     2341 aaaaagcata tgaacctatt tttacaactt attgattgac agcagatgga ggaaaaaaat
     2401 gaatccatat gaacaactta ttcaagattt gatcgatgga aaaatcgaaa agattgatgt
     2461 aaaaagagaa gaattaatgg cttttcgaga agcgtggctg aagttggaga atcgtaaata
     2521 tgtggtggga aaagcctcac tgaacggcaa tgtgacttat cattacgatc cgacacgttt
     2581 attttagtat gaagtattca gaaaagaacc aaag
//
LOCUS       DAYYCY010000224         2589 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.229, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000224 DAYYCY010000000
VERSION     DAYYCY010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2589
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.229"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..431
                     /locus_tag="ACHAZB_002645"
     CDS             <1..431
                     /locus_tag="ACHAZB_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HFM8615259.1"
                     /translation="PNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTILKECKFMI
                     GNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKIQDPQFIKS
                     LKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            404..1081
                     /locus_tag="ACHAZB_002646"
     CDS             404..1081
                     /locus_tag="ACHAZB_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFM8615260.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            1032..2213
                     /locus_tag="ACHAZB_002647"
     CDS             1032..2213
                     /locus_tag="ACHAZB_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFM8615261.1"
                     /translation="MAIQLKLRKKELILNSRILLSSPHMGGTEEKYVAKAFKDNWIAP
                     LGENVDKFEESITKYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATV
                     NPILYQGAVPVFIDSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYER
                     ILAIANQFNVPVIEDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSD
                     KKYVEHSRFLATQARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIF
                     NRYKEAFSGIDRIEMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRA
                     LWKPLHLQPVFQGVKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIF
                     K"
     gene            2226..>2589
                     /locus_tag="ACHAZB_002648"
     CDS             2226..>2589
                     /locus_tag="ACHAZB_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8615262.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLK"
BASE COUNT          965 a          332 c          508 g          784 t
ORIGIN      
        1 tacctaatag tgatgctggt agcgaaatta tcagaaaaat gtaccagaaa tatttaaaaa
       61 cggatattat ttatattacg aatttagagc cggaggaata cctgactata ttaaaagagt
      121 gcaaatttat gatcggaaat tcttcttctg ggataattga agctgcatca ttcaaaatac
      181 cagtaataaa tttaggaacc agacaaaatg gacgagaaca atctaaaaat attataaatg
      241 caaatctaaa tgaaaatgaa attgaaactg ctttgaataa aatacaagat cctcagttta
      301 taaaaagctt gaaaaatgtt aagaacatct acggtgacgg caacagttct agaagaataa
      361 caaaaacatt gaatcaaata ttgaatgatg gggaagatat taagtggatt caaaaacaaa
      421 tagcttatta aaatatacta gtgatccata cattattatt ggggctggcg gacacgcaaa
      481 agtcattatt gaaaccttaa gattagaaaa taagttaata tatggatttt ttgatgataa
      541 gcaaaataaa atgcaagatc tgaaatatct tgggaaaata aacgaattga atagctgggc
      601 ggatagcaaa aaaaaaatct ataaatttat tgtagctatt ggtgactgtg tagtaagaaa
      661 aaagattata aaaaaattga atatgccaag tgagcaatat ggaatagctg ttcatccaag
      721 tgctatagtg gcaaaagaaa tttcaattgg ttatggaact gttatttttg cgaatgctgt
      781 aattaaccca gatgctgtaa ttggagagca tgctataata aacacaggta gcattgttga
      841 gcatgattgt agaattaata actatgttca tttatctcca ggtgtatgct tagcgggtgg
      901 agtacatgta ggtgtaggaa ctcaaatagg aattggttct caatgtatcc agatgaagaa
      961 aattggatct tggtgtatga taggcgcagg gagcactatt gttaaggata ttccctcaca
     1021 ttccttagct tatggcaatc cagctaaaat taagaaagaa ggaattaatt ttgaattcta
     1081 gaattttatt atcaagtcct catatgggag gaacagaaga aaaatatgtt gctaaggctt
     1141 ttaaagataa ttggatagcg ccattaggtg aaaatgtgga taaatttgaa gaaagtatta
     1201 caaaatatac tggttctaag ggcggagctt ctgcgttaag ttcaggtaca gctgccatac
     1261 atcttgcctt aagactgctt aatgttggaa aaaacgatat tgtctttgct ccttcattta
     1321 cttttatagc tacagtaaat cctattttat atcaaggggc tgtaccagtg ttcattgact
     1381 cagaacctaa tacatggaat atgagccctg ttgcactaga gagcgcatta gaagaagctg
     1441 aaagaaacaa ccaattacct aaggcaatca tcgtagtgaa tttatatggt caaatggctg
     1501 attatgaacg aattttagct attgcaaacc aatttaatgt tccagtgata gaagatgcag
     1561 cggaatcgtt aggttctact tataagggaa tttcgtcagg cttatttggg acaattggga
     1621 tctattcatt taacggtaat aagatcatca caacctcagg tggaggagct ctagtgtcta
     1681 gtgataaaaa atatgttgag cattcaagat ttttagcaac acaagcaaga gatcaagcac
     1741 cgtattatca acatagcgaa ataggttata attaccgtct aagcaatatt tctgccggta
     1801 tcggacgtgg acaaatggaa gtattgaatg atagagtagc taaacgaaga gaaattttca
     1861 atcgatataa ggaagctttt tctggaattg atagaattga aatgatgcca gaagtggatg
     1921 gcatggtgcc aaatcgttgg ttgtctactt taacgatcaa tgattataat tctattgatc
     1981 aagtagttaa cgtaataaat aaactaaatg aaaaaaacat tgaaacaaga gctttgtgga
     2041 aaccactaca tttgcaacca gtatttcaag gggtaaaaat gtattaccat gataataatc
     2101 ttagtgtgtc agaaaagtta tttaatagcg gcttatgctt accttctgga agtaatatga
     2161 cagatagtga acaagaattt gttattgatg agtttaaaaa aatattcaag taggtgtgat
     2221 attttatgaa cataggaatt attggtttag gttccatggg gaaaagaaga cttagactca
     2281 ttcaagaaca ctttaatgat attttactat gtggtgttga tactaatgag gcgagacgaa
     2341 aggaagttga aaaggaattt ggaataccaa cttataatag tctaacagag gcaaaaaatt
     2401 tgtttgaaat aaattcagtt tttgtttgta catcccctat aacccatgaa tttattataa
     2461 aagaggcttt aaatttagat tgtaatatct ttacggaaat caatttatta aatgaatatt
     2521 atgatgaaat aatcgaagta gctaaaaaga aagatagatt actttatcta tcttctacat
     2581 ttttgaaga
//
LOCUS       DAYYCY010000225         2589 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000225 DAYYCY010000000
VERSION     DAYYCY010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2589
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.230"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            257..532
                     /locus_tag="ACHAZB_002649"
     CDS             257..532
                     /locus_tag="ACHAZB_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8615263.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            545..>2589
                     /locus_tag="ACHAZB_002650"
     CDS             545..>2589
                     /locus_tag="ACHAZB_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFM8615264.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     "
BASE COUNT          777 a          407 c          562 g          843 t
ORIGIN      
        1 tttttgtcgc agccccattt ttttgttgtg ataaaagaac aagaaaggca ggaatgggaa
       61 agtaaacatc cttttgtcag aaacttttat ctttcttaag ttttacttga tatctgatac
      121 tatttggaat agaatgaggc gttgaaaaat ttatggaaat ttctaactag caataaacga
      181 tgaaacaatg aaatattttt catccaaaaa gaactttgct ataatgatgg aagtttgtgt
      241 ataggagatc tggaaaatga gagagagttt tgaacaacaa aaaaaactgt tgcatgatcg
      301 ctatggcgcc ctttcgatgg atgaccgccg tcaaatactt tgtaagttgc gtaaaagaaa
      361 tatattgatg tatcgtcagt tggaacgtct gaaacatgat ctgcttcgtt tggagtcaaa
      421 acgtgtacag tgtgaattag aggggaacca aacacaggtt gaagtagtcg aaaccaaaat
      481 tttaaagaaa aaagaacaat ttttaaaaat gcttacacaa aataaaaaat aaaggggatg
      541 gacgatggaa tttgctgagt tagttattgt atttgcagta acgatcacct tatcgaacat
      601 agctagccgc attttgccaa tgattccagc tccactgatc caaattttcc ttggagtggt
      661 attaggtctg acagagtggg gacaatcaat cgattttgaa cctgaattgt tcttagttat
      721 gattattgca ccattgttat ttagagaagg agaaaaagca gatatttctt cgattctcaa
      781 aaatttcggg acaattttgt tcttggcatt tggaggagtc attttgaccc ttgtcggggt
      841 aggtgcgacg ttatcattcc tgttgccaag tgtcccactt gctgcctgct ttgcttttgg
      901 agcagcgtta ggtccgacag atgcggtagc agtcagttct ctttctggac gagtgaatat
      961 ccctaaaaag gcgatgcata ttttggaagg agaaggtctt ttgaatgatg catctggtgt
     1021 tactgccttc cagtttgctc ttggtgcgct gatcaccgga tctttttcag cagtaaacgc
     1081 ggggatgagt ctagtagttt ctagtattgg gggagctttg atcggctttt tgcttgtctg
     1141 gttcaaacaa aaaataattc atttgatcga aaaagcatca gcacaagatg tcacagggta
     1201 tctgctgatc gaacttttgc ttccttttct agcctatgta ttggcagagg catttgatgt
     1261 atctggaatc attgctgcag tggcagcagg tattctccaa gcttctggtt ttaggaaaat
     1321 ctctgtgttt gatgcagaat tgtcgagctt gtcccacagt acatggacaa ctattgcttt
     1381 tacactgaat gcattagtat ttatattttt aggaatcgaa ttgacgcaag tgttctcgcc
     1441 agtatgggga gacggtttat atccaaacgg tcttttatta gcgattatcg tattaatctc
     1501 tgtcatgctt tttgtgatca gattcatttc tatcagtctt ttttatgtat tcaaagatgg
     1561 cagtaaaaag ttcaaaaaac agttgaacga gatcttgatc ttaacatttg gcggtgtaaa
     1621 gggaacagtc agtttggcta cgatttttat tttgccattt tcaatcaata atatgatgtt
     1681 ttaccagcgg tctttgcttt tgtttctgac ggcgggtgtc atcttagtga ctttggtcat
     1741 agggatcatt gtcttgccta tgctgacgga aacagaggaa gcgaaaagca cggatttgaa
     1801 tgctttgatg attttagaag aggtcgttga agttttgcga aaagaaataa aggaaattga
     1861 tcgtaataca aaagaatttt tagccactga agcagtgata gaaaattatc aagaacggat
     1921 acgtgatctg tacttggaag atttaaccga tgatgaaaag caagaagtac aagaaataca
     1981 agcactgatt ttgtcgatcg aacgggatgg attggatgaa agttatcgtt cgggcaagct
     2041 ttcttctaat ggttatcgtt tttactcccg ttttctttct cgattcgaac attctatcac
     2101 aagccagatt ttgtctttta tcggcttttg gttcattgtt gtccgtaggt taatgcgaat
     2161 cgttctccat ccgaaaatgt tttggcaaag aagatatgct gatcgtcaga catttattag
     2221 tgatgaagat atccaagaaa tcagagagac atatcaaaaa aatactcagt tgattattga
     2281 aagtttagat aacttgactg atatttatga tgaaacgatg gttcaatttt ttatccagca
     2341 acgcaaactt gaaggaataa aaatgaactc ggggaatttg atttcttcgt ggatgatcca
     2401 gcaagatagt ctgtttacga aaaaaatgct aagaggttat tatcttgaaa gaaaaatcat
     2461 tgatgaatac gaagttgctg aaaaaatcac gacgttttct gcgaacagct atcgcaggaa
     2521 tatcaatttg ttagaatctt atacgatgaa caaaccatct gataactttt ctttccgatt
     2581 tgcgtttcg
//
LOCUS       DAYYCY010000226         2586 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.231, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000226 DAYYCY010000000
VERSION     DAYYCY010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.231"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1451
                     /locus_tag="ACHAZB_002651"
     CDS             <1..1451
                     /locus_tag="ACHAZB_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFM8615265.1"
                     /translation="KKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTEM
                     SNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSNA
                     ESSTTSSSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFST
                     PQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIAQ
                     AILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYENY
                     EESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNELI
                     KTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIHA
                     VKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGSTG
                     NSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            1800..2432
                     /locus_tag="ACHAZB_002652"
     CDS             1800..2432
                     /locus_tag="ACHAZB_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HFM8615266.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
BASE COUNT          963 a          375 c          509 g          739 t
ORIGIN      
        1 tgaaaaaatc tttaaaactt gcatcagcag ttatattaat gtcccaactc cttatagcaa
       61 cacctttcca tgtgttggca agcgaaaatt caacatacaa aattgcaaaa gaaagaaccg
      121 agacgactga aatgagtaat tccgatgtaa cagcagagag tagtgaactt ttgacaactt
      181 catcaagtga aagtagtacg acttcaaatg ccgagtcttc aacaacttca tcaagtgaaa
      241 gtagtacgac ttcaaatgcc gagtcttcaa caacttcatc aagtgaaagt agtacgactt
      301 caaatgccga gtcttcaaca acttcgtcaa gtgaaagtag tacgatttca agtaccgaat
      361 cttcaacaac ttcaagtagt acaagtgaaa taacttcttc gaaagtgaca aaaccaaacc
      421 atatagaatc ttcaaaaaag gaagcgccaa aaacggaagg acaaacaaaa actttttcga
      481 caccacagca aatagaatca acagaatctt tccaatcaca accaatacta tctggtaatg
      541 aagaaattca ttttgacaaa aatgaatctg tagaaaattt tattatgaaa attggtgagt
      601 ctgcgagaaa aattggtcag aaatatgatt tatatgcttc tgttatgatt gcacaagcga
      661 tcttagaatc tgccagtggt caaagccagt tagctcaggc accgaattac aatttatttg
      721 ggataaaagg aacttataat ggaaactttg tcattatggt aacaaacgaa gatttgggta
      781 atggaacgct ttatacgacc caatcaaaat ttagagtgta tgaaaactat gaggaaagct
      841 ttgaagatta tgccaaatta ttaactaagg gaattagtgg aaataaagat ttttatgcag
      901 gagcattaaa agctaatagt aaaacatatc gagaagcaac taaattcctt acaggacggt
      961 atgcaacaga tacacaatac tatttgaagc taaatgaatt aattaaaacc tacgatttaa
     1021 ccaactatga taaagaagtt aaaggtccac aaatgagtaa gactggatac caagtgccta
     1081 ttcataatta ttcaataacc agtttgtttg gaaatcgtgg aaatgaattt caccgaggac
     1141 ttgatatggc agctacacag ggtgagccga ttcatgcagt taaagctggt aaggttgtta
     1201 aagcagagtt tcactactct tggggaaatt acgtggtgat acaacacgaa gatggatcaa
     1261 ccgcgttata tgctcaccag caacaatacc tagtaaaaac tggagataat gttttgcaag
     1321 gacaaataat tggctatgta ggaagtactg ggaatagtac tggcccacac cttcatttgg
     1381 aaatttgcaa agatagtagt ctttctcaga gcatgttagt tgatccgaaa accttattat
     1441 tcggagacta aaaaaggtaa tcaatctgac aaagtaacaa gaaaatggtt ggattcttag
     1501 acctttgatc aggatattga gactggaatt gatatctcga ttacgatggg tttgacaaat
     1561 aaaacaagtc cattatcgaa tatcgagtgt attattgccg tctttatgct catattttga
     1621 gcaggtttca ctagatgaaa acaatttatc tatcttgagg atttcgtgta cgtatcgttc
     1681 agtcttgtat tgtaaattag tcataaaatc agactaagaa aaattggaaa tatttttttc
     1741 tgatttatta aaagttaatt gaaattatat ggtacttctt tcttgggagg gtaggtgcag
     1801 tgaaggaaag atatggtcac cttactgttg ttgaggagat tactcataat ggatataaaa
     1861 aatatcgttg cctatgtgac tgtgggaatg aaacgattgt atatcgatca aacttactat
     1921 caggtagaac caaaagttgt ggatgtggaa attataaaaa taggcataaa tataaaaatt
     1981 tgacaaataa aacttttggt aagttagttg caatcaagcc aactaaactg cgaagtgaag
     2041 gaactgttgt ttgggaatgt caatgtctgt gtgggagaaa aaagttagta tcggcaaagc
     2101 gacttctaaa aggtgatgta aaaagctgtg gatgtttgaa atcaaaaaag catcacattg
     2161 aaaataaaaa attcggatta cttactgcaa agtatccttt ggacaataac tgttcatgga
     2221 aaacaaaatg ggtttgtcat tgtgagtgtg gcagtatgtg tgagataagt tatagtaact
     2281 tattttttgg acatactcaa agttgtggat gtttaaaaag gcaagaaaca aggacatgtg
     2341 ttgaagggac agtaatagag tgtctaacgt caaaattacc gaaaaataat acaagtggtg
     2401 ttaagggagt ttatcgttct aaagagaaat agattgcgta tataacttta aaaaaaaaga
     2461 ggtactattt aggatcgtat gattctatta agagtgcagc tcaagctaga aaagatgctg
     2521 aaaataaatt gtttcaacct ttgattgaca aatatcgagg taatcattaa ttattgtgga
     2581 taataa
//
LOCUS       DAYYCY010000227         2562 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.232, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000227 DAYYCY010000000
VERSION     DAYYCY010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.232"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1323
                     /locus_tag="ACHAZB_002653"
     CDS             <1..1323
                     /locus_tag="ACHAZB_002653"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFM8615267.1"
                     /translation="NAAIYSSFLLKFRGEIPLRNLETFQHSAIIISVIFIALNILLGA
                     YVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRWFAFPRLVILLSFVLGTLFLSIYR
                     ILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSKKNNKHKVEAVILDNYYANIKNMI
                     KDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNTTFENLTLINPNIMNFEDESIIEA
                     SGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFTAILVKVTSPGPIIYKQVRITKNQ
                     KEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVGKFIRAVRFDELPQIFNVLKGDMS
                     IVGPRPERSFFVDQFNAENPYYYLRHNVRAGITGYAQVYGKYVSDYNSKLRFDLLYIK
                     KYSLMLDLKILLQTIKILFDKMSSQGLEEDENVISLAEIAFPNEKIFR"
     gene            1339..2379
                     /locus_tag="ACHAZB_002654"
     CDS             1339..2379
                     /locus_tag="ACHAZB_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM8615268.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            2398..>2562
                     /locus_tag="ACHAZB_002655"
     CDS             2398..>2562
                     /locus_tag="ACHAZB_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HFM8615269.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERL"
BASE COUNT          887 a          405 c          475 g          795 t
ORIGIN      
        1 aatgcagcaa tatatagtag tttcttactg aagtttcgtg gagagatccc attacgaaat
       61 ttagaaactt tccaacactc agcaattatt atttcagtga tttttattgc gttgaacatt
      121 ttgttgggtg catatgtttt ttataatcga atgatcagtg acatcgtgtt tgtcacagtc
      181 atcggtcagg tattgatgac actagggatc atggtagtga cttttgcggg ccgctggttt
      241 gcttttccac gtcttgttat cttactttca tttgttctcg gtacgctttt cttaagtatc
      301 tatcgtattc tcgtatataa actttattta cgcgtaagtc gagataaaaa ggtcgtcatt
      361 attgggttag aaaaggatgt cgcaccagca atccaaaatt ttggttcaaa gaaaaacaac
      421 aaacataaag tcgaagcagt cattcttgat aactattatg caaacatcaa aaatatgatt
      481 aaagacatcg atattgtcta tcttgcttct catatcgaag aaactgaaaa aatcaaaatc
      541 tatcaattag ttactaaaaa tgaaaaaaaa ttgtttttga atacaacatt tgaaaacttg
      601 acattgatca atccaaatat tatgaatttc gaagatgaaa gtattattga agcatctgga
      661 tttagaatcc aacctgaata tgaactattc aaacgtttgt ttgatatcat cgtttcactt
      721 gttttattag ttattgcttc accatttatg ttattcacag caattttagt caaagtgaca
      781 tcaccgggac caatcatcta taaacaagtg cggatcacaa aaaatcaaaa ggaattttca
      841 atctataaat tccgctcgat gaccgccaca gccgaagcaa aatctggacc agttttagct
      901 aagagcaatg atgcacgagt aacgcctgta ggtaaattta ttcgagctgt ccgctttgat
      961 gaattaccac aaatcttcaa tgtgctgaaa ggcgatatgt cgatcgtagg accacgtcct
     1021 gaacgttctt tcttcgtcga tcagttcaat gcagaaaacc cttattacta cttacggcat
     1081 aatgttcgtg ctggtatcac tgggtatgca caagtctatg gaaaatatgt ctctgattac
     1141 aacagtaaat tacgttttga tttactttat atcaaaaaat attcattgat gcttgattta
     1201 aagatcttgc tgcaaaccat caagatccta tttgataaaa tgtcctctca aggacttgaa
     1261 gaagacgaaa atgtgatctc attggcagaa attgcttttc cgaatgaaaa aatctttcgc
     1321 tagttgggag gcgtaaaaat gaacaatccg aaattcagca taatcgtacc agtatacaat
     1381 gcagaagcaa ctattggtcg aacgattgat attctaaaag aattggatta tgaagagtac
     1441 gaagtcgttt tagtaaatga tggatcaaca gatcaaacac aggaaatcat tcaagaactg
     1501 attgaaaatg atgaacgttt tgaattgatt actacgacca atcaagggcc cggattagca
     1561 agaaacgaag gaataagaca gtcaaaagga gaatatctct tattcttcga tgcagatgac
     1621 acgccagtaa aaacgatctt gtcagattat gatcatttgc tagaaaagag tccaaaagcg
     1681 gatcttgttg ttagttcgtt tgtttttcgt acaatggacg agggaagaat cgtttccgaa
     1741 aaagaaaact tagttacaga atgtcactat ctaactcacg aagagtttat aaaagacatg
     1801 tatcagttaa tgaatgaaca gttgatgtat gttgtatgga acaaatgcta tcgtagcgat
     1861 attctgaaaa ataaccaaat tctatttaaa acttataata gttgtgagga tcgaatattt
     1921 aacttacatt attatgaata ctgtcatcaa gtgatcatga acccaaaagt agaatatatt
     1981 tatgaatttg aaggtgggaa ggggatcact aatcaatacc gacctaacaa gttcgaaaca
     2041 tttaaagaat tctattcatt agcaaatact gtaacagacg atattaataa agacggtatg
     2101 gcggcgcttc ttttaaaagg gacaacttca gttatttttt ctatttatag tacctctgca
     2161 agaacttcaa aagagaagaa agacgaagca aagcagatat tgagtgatcc agtgatccga
     2221 gaagccaagc aaattgctaa gacggattct tcagcaaaaa aaataacaaa gttgctttac
     2281 aatatgccag ctccactatt tttatctgct gtaaaagttg gaagcttcgt tgaagtgaaa
     2341 atgccagggt taatggctgc tttgaagcga gtatattagt taatggagga aaatataatg
     2401 aaaagaattt tgcattttca gggacgaatg ggtttaggtg gagcagagtc tttcatgatg
     2461 aatttatatc gtaaaattga tcgtacaaac tatcaatttg attttttgat ttatgaagat
     2521 tatgcagatg ttcaagacta tcattcagag attgaaagat ta
//
LOCUS       DAYYCY010000228         2423 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.233, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000228 DAYYCY010000000
VERSION     DAYYCY010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.233"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            120..2273
                     /locus_tag="ACHAZB_002656"
     CDS             120..2273
                     /locus_tag="ACHAZB_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HFM8615270.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          827 a          425 c          536 g          635 t
ORIGIN      
        1 aaagttgatt ttatcttgaa attaggtaaa gaatttcttg ctccgcccat tttttctttt
       61 ttattaagaa gtttttgggt acaatgaagc atgaatgtaa ataggcggaa ggataggaaa
      121 tgatgaaaga ttttatgaaa aaaatattaa atacttcttg gttagaaaaa ataagaaaag
      181 gtgcacaaga acctaagaac aaaacgtttc gccggtccca tgttcctttt cgcttaaatt
      241 ttcttttttt cattatattt acgttatttg ttgcgttgat tgctagattg ggttatttgc
      301 agatcgtgaa cggcgaagag atggaggcgc gagtgaagtc tacttctacg attacggtcc
      361 aagaaagtac tcctcgcgga atgatctacg actccacagg aaaagcttta gttacgaata
      421 aagcgaatca ggccatcaca tatacgagag gaacacaagt tacggctaaa gatttgcttg
      481 aaatcgcaac gaagctgaac tcgctgatcg atgttccggt agatgaaaat ctgacagata
      541 gagataaaaa agatttttgg ctggctgatc ccaaacattt gaaagaagcc acaaaaagac
      601 tttcggcaaa agaaaagcaa ttagggaaca gcgaacaata tagtgcgatg gtcaataaag
      661 tcacagctga tgagattaat ttaaacgagg agcaattaaa aattgctacc atctttaaac
      721 ggatgaatag tgcatatgaa ttaaacactg tctatatcaa aaactctggt gttactgatc
      781 aagagttagc tgttgtggca gaacgcgctt ctgaacttcc aggagtttcg actggcacag
      841 actggacacg agaatataat gcagcaagtt cactgaaaag tattcttggc agtgtgacga
      901 ctgaaaagca aggacttcca gcagatgaag cagagaaata cctagcaaaa ggttattctc
      961 ggaatgaccg tgtaggacaa agttatttgg aaaaacaata tgaagatgtc ctgcaaggaa
     1021 ccaaaaccca atatgaagtt tcccttgata atgaaggaaa tgtttcgaat caaaaagaaa
     1081 tcttctctgg ggaaaaaggt tctaatttaa tgttatctat gaatgcagaa ttccaatcaa
     1141 aagtagaaga aattttgaaa aggaattatc aaacattgat caacaacggc aaagcacagt
     1201 attcgcctgg tgcttatgca gtggctatga atcctcagac aggagaagtg ctagcaatga
     1261 ctggtttcag tcatgaacaa ggttccaaag aaatcaccga gaatgcttta ggcacgatta
     1321 ctagcgcctt tgatccaggg tctgtagtca aggctggtac tttgacagca ggctgggcaa
     1381 gtaacgcgat cagcggaaat caagttttga ttgatgagcc gattcgtctg caaggtgctt
     1441 cagaaaaaag ctctgtcttt aatcgaagcg gacaagttgc tcttgatgca gtcaaagcat
     1501 cggaattgtc ttctaatacg tatatgatca aagtggcgtt aaagatgtta ggactagact
     1561 atacacctgg aatgggacta cctagtttag atgaagaagc taaagcttat cagcaattga
     1621 gagactcttt caaagagttt ggtcttggta caacgactgg gatcgatttg ccgaatgaat
     1681 ctcctgggat ctcacgttct gttgattata tgaagaagtt caatgcggat aatggcaaag
     1741 aatggtatgc tccaggtaac tttaccgatt tggcatttgg acagtttgac acatatacac
     1801 caatccaatt agctcaatat gcttcaacag tcgctaatgg cgggaaacga gtacagccgc
     1861 acctagtcaa agcgatctat ggcaatgacg aaaatggaaa cttgggtgaa gtgaagaaag
     1921 aaattggcac aacagtagaa aatacggtca atatttctgc agaaaatatg gcgatcctcc
     1981 gtgaaggatt ccaccaagtt gtccatggta ccgatcctta cacaacagcc aaaccattag
     2041 ccagtgcgaa aatggacctt tccgcaaaaa cagggactgc agaaacggtt gcggaaggtc
     2101 atccagatat tgcgacagtc aatagtaata tcgtggcata cggaccaaca gacaatccgg
     2161 aaattgccat cagtgttgtc ttgccgaact tattagacga acaagaccac ataaacttga
     2221 cgattgctaa agaaataatg gacgcgtatt atgatatgtt catggcaaaa taaaaaagag
     2281 gtcgtgaaaa agccgcttag tttcgagtat gaagaaaaat tttgtgaaaa tagcttctca
     2341 catttttgct taaatttgac ttcataccga gaaactggct tttgaacacc gtttactcgg
     2401 caaaaaaggt tgtgacaaaa att
//
LOCUS       DAYYCY010000229         2340 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.234, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000229 DAYYCY010000000
VERSION     DAYYCY010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.234"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..107)
                     /locus_tag="ACHAZB_002657"
     CDS             complement(<1..107)
                     /locus_tag="ACHAZB_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM8615271.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGR"
     gene            177..527
                     /locus_tag="ACHAZB_002658"
     CDS             177..527
                     /locus_tag="ACHAZB_002658"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8615272.1"
                     /translation="MSRLIIQTAFYKAIIPENLDEVKLYLWEDNGEIVGFSGVSAEEL
                     VMLFLDPDFIGKRYGSQILIELIETEDIKRIDVNSQNKYAKHFYLSHGFEIESEEDLD
                     GFGKPYPITHLVKK"
     gene            941..1759
                     /locus_tag="ACHAZB_002659"
     CDS             941..1759
                     /locus_tag="ACHAZB_002659"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8615273.1"
                     /translation="MNKNPHNLKLVAVDMDGTFARTDYTYDIPRFKAVLSRMKAVDCQ
                     FVVASGNQYYQLRSLFPNYYDELSFVAENGAFVKDKTEVLFSADMPRKAIEVVLDLCK
                     DYPEVKNVMCGLNSAYCERGKVSQAFFDLTNIYYHRLEWVADFAEVQDKILKFAPTMP
                     VEKTEFYVELFREKLAGLLVPTSSGHGSVDLILSECHKTFGLKMLVERLGINPDQCVA
                     FGDGGNDIEMLEYCGLSYEMDNATEAVKQVAKHQCPSNDEDGVLVTLDRLFPNT"
     gene            complement(1832..2191)
                     /locus_tag="ACHAZB_002660"
     CDS             complement(1832..2191)
                     /locus_tag="ACHAZB_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFM8615274.1"
                     /translation="MSDNLFLEVQPEVFKQVPRLKDGFLLTLNDGSNQFSSAEGCCMI
                     GDHFLLVSTQHYIPAYDLTIPCEEATVKTSEYDTTFINGHLVLEVTPSNQTIRLKNES
                     GILDSNVQVYFDSSDAS"
BASE COUNT          766 a          341 c          447 g          786 t
ORIGIN      
        1 tggcgtccac gtttaagctg cttcgcaacg atggcaaccg gagtttcttg atgaaaatat
       61 gcttctatca ttacaagctc gtttggtgta agatgggtat aagtcattta tgttcactct
      121 ccttgtatgc tttagcgggt attacaattt gagtgtaaca taaatggctt ttttatttgt
      181 ctcgcttaat tatacaaacg gcgttttata aagcaatcat tcctgagaac ttagatgagg
      241 ttaaacttta tttatgggaa gataacggag aaattgtcgg ttttagtggt gtaagtgcag
      301 aagaattagt tatgctattt cttgatccag attttattgg taagcgatat ggtagtcaaa
      361 tattaattga gctgattgaa actgaagata taaaaagaat tgatgttaat agtcaaaata
      421 aatatgcaaa acatttttat ttaagccatg gatttgaaat tgaatctgaa gaagacttag
      481 atggttttgg taaaccatac cctataacgc atttagtaaa aaaataattc atgaatggtt
      541 tattacctga ttgaacatga ataaaagtaa tatttttatt ttgatggcct atttctaggt
      601 tatgaattat ccagatcaaa tttgtgtatt acattcagta ataaggtgct tatcttagga
      661 tcaacttatg atactagctg accaaggatg taccagttat agaaaatcag ttaaagtaaa
      721 aatgataaaa atcttatgtc aacactgatt taggctgacc tgtgggttag gagaaagaca
      781 ggtcggtagc ttttaataca aaaaatggct tgtatgtatc tagatagtta actgaaatta
      841 gaaatgttct tatttttaac ggagtaagaa aaataggctg ctttaggtct gaatactttt
      901 tcaatataat gaagatatca aaggcgacag gagtgttttt atgaataaga atcctcataa
      961 tctaaagttg gtagcagtag atatggatgg aacatttgct cgtactgatt acacatacga
     1021 tatcccaagg ttcaaggctg ttttatcgcg aatgaaagct gttgattgtc aatttgttgt
     1081 tgcaagtgga aatcaatact accaattgag atcgttattt ccaaattatt atgatgaatt
     1141 aagttttgtt gctgaaaatg gtgcttttgt taaagataaa acagaggtac tattttcagc
     1201 cgatatgcct agaaaagcga tcgaggtggt ccttgattta tgtaaagact atcctgaagt
     1261 aaaaaatgtg atgtgtggtc tgaatagtgc ctactgtgaa agaggaaagg tgagtcaagc
     1321 attttttgat ctaaccaata tctattacca tagattagaa tgggtagctg attttgcaga
     1381 agttcaagat aaaattttaa aatttgctcc gactatgcct gttgaaaaaa ccgaatttta
     1441 cgttgaattg ttccgtgaaa aattagccgg gctattagta cctaccagta gtggtcatgg
     1501 ttctgtagac ttgattcttt ctgagtgcca taaaactttc ggcttgaaaa tgttagttga
     1561 gcgcttgggg attaatcctg accagtgcgt tgcttttgga gatgggggca atgacattga
     1621 gatgttggag tattgtggtc tgagttatga aatggataat gcaacggaag ctgttaagca
     1681 agttgctaag catcaatgtc cttcaaatga cgaagacggt gttttagtga cgctcgatcg
     1741 attgttccct aacacgtaaa cactaggtaa ttcatcaaaa aacctcgata taagaaatat
     1801 tcgaggtttt aaaactatat ttagaacaga tttacgaagc gtctgatgag tcaaaataga
     1861 cttggacatt gctatctaaa ataccacttt cattctttaa acgaattgtt tgatttgatg
     1921 gagttacctc taatactaaa tgcccattaa taaaggtagt atcgtattca gatgttttaa
     1981 ctgttgcttc ttcgcaaggt attgttaaat catatgctgg aatataatgt tgtgtggaaa
     2041 ctaataaaaa atgatcacca atcatacagc atccctctgc tgaagaaaat tgattagagc
     2101 catcatttaa agttaacaag aatccatcct ttaaacgagg tacttgctta aatacttctg
     2161 gttgtacttc taaaaataaa ttatcgctca ttttcacgac caccatcgac ttccacagtt
     2221 gaaattgaca ttttatttaa cacacctatt cccccttaac tataactcaa ttctatataa
     2281 aaaatattct attctcaaat aattgaactt ttcaaatcgc caatttactt cggctttatc
//
LOCUS       DAYYCY010000230         2309 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.235, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000230 DAYYCY010000000
VERSION     DAYYCY010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.235"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..958
                     /locus_tag="ACHAZB_002661"
     CDS             <1..958
                     /locus_tag="ACHAZB_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HFM8615275.1"
                     /translation="SALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQV
                     KMQTAQVANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNE
                     ISCTIPSHSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRV
                     TYLPQESFFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEG
                     GNNLSGGQRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIA
                     HHLPIAKACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            927..>2309
                     /locus_tag="ACHAZB_002662"
     CDS             927..>2309
                     /locus_tag="ACHAZB_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFM8615276.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQ"
BASE COUNT          890 a          343 c          421 g          655 t
ORIGIN      
        1 cagtgcatta attttatggc taggttctta ttttgttatg gataatagaa ttagtctagg
       61 tcaacttatc acatacaatg ctttactagt atttttgaca gatcctttac aaaacattat
      121 taatttacaa gtgaaaatgc aaacggctca agttgcaaat aatcgtttaa atgaaatttt
      181 cgcgattgat tcagaaaaat cgaaccagtc ttttgaatat atgctttctg aacaattatt
      241 tcaacaagat atcactgtaa aaaacgtttc attctcgtat agcatgaagg ccccaacgtt
      301 gaacgaaatt tcatgtacaa ttccttcgca cagtaaaacg gctatagttg gagttagtgg
      361 ttctggaaaa tcaactcttg caaagttatt tgttcgtttt tatgaagcat cagaaggatc
      421 gatccattat ggaaacatta atcacttaga cattcctttt cagatattac gcgatcgagt
      481 gacatatttg cctcaagagt catttttctt tagtggaaca atcattgaga atcttatatt
      541 cggtttagat agcccaccaa gtttcgaaca aataatagaa gtttgtgaga tagtccaact
      601 aaatgaattt attaatcaac agccattacg ttacgagaca ttgttggaag aaggtggaaa
      661 taatctttca ggagggcaac gacaacgatt agccttagca cgggctttat taaaaaatcc
      721 ggacatatta attttagatg aagcaactag tggattagat accttgttag aacatgcaat
      781 tatgaattat ctcctcaatt taaaagaaaa aacgattatt tttattgcgc atcatttacc
      841 aatagcaaaa gcatgtgatc aaattttagt tctccatgaa gggcatttgg tagaaaaagg
      901 aacgcacgat gaacttcgtt ttgccgatgg tatctatcaa aaattatggg aaatctagga
      961 gagggaaaca atttatgcag aataataatt ggttcgaaca ttctagtatc tatactaaac
     1021 aacatcatag cttctaccgt tgggtgttat accctgtaat tcttttttta tgtattattg
     1081 gattgttttt aacctttgct aaaaaagagg ttgttattcg gacaacggct caattgaacc
     1141 ccgaaaaaat agagaagtta caagtgccat tagaagctaa aataatggaa aacttcctaa
     1201 aagaaaatca atttgttcat aaggacgatg tgttggttag attggattgt tctttaatag
     1261 agaatgaaaa ggctcagata gaacaagaaa accaacgtat tactcaacaa ataaagatgg
     1321 ctcagctatt tattgaaagt ataagtaaag gaaaaaattt gttttcaacg gatgacagtt
     1381 ttggctacag taatcaatta aagagcatgt tgtcagaaaa agaatcactc cgctacgctt
     1441 tgaagcaaag tgaattaaat gatcaaaagc aattagaagt atacgaaaag acaaaaagac
     1501 aactagaaaa acaaattgag agttcagata gtaaattaca agaatggcaa caagtacagg
     1561 tagcttggag taataatcaa tcattaaaag atttttcaaa agaaatgatg gcaaactatg
     1621 agaattggca agaacaacta aataatgttt ctgatgatca aaaaaatcaa gtgaaactga
     1681 caatttcagc aagcataaat gaacaaattg agcaactaaa aaaagaagta gaacagtatc
     1741 agtcagaaaa agctaaatta gttaaaccaa ctacttctga gaatgacaga attagtcaaa
     1801 cggaaaaagg aaagcaagag ctagaacaag caataacaac tgtaaaacaa acagttacag
     1861 agctaaatga aaaacaagag aagaaccagt cagttatcaa aagtttggac gatcaacttt
     1921 caaaaggaat actaaaagct cctgtatctg gaacggttca tttaaatgaa gaaacaaagg
     1981 gacaagcaga actagccaaa ggaacagtgc ttgcagaaat ttatccgatc cacgaaaaat
     2041 caaaaatgaa atttacagca cttttacctg cgaatgaaag tacctatatt aaagaaggga
     2101 tgaaggttca ttttaaattg gatcaaaaag gtggggctcc gattacgatc gacggaactt
     2161 tagatgaaat ttcagaaaat tctactgcca ctgagaaagg tgttttttat gcagtgaaag
     2221 gaacgttaaa aacaactcaa tcgtttccgt ttcgttatgg attaactgga gagttgtctt
     2281 taattgtagg agaaaaaaca tattggcaa
//
LOCUS       DAYYCY010000231         2294 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.236, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000231 DAYYCY010000000
VERSION     DAYYCY010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.236"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            161..1270
                     /gene="pdhA"
                     /locus_tag="ACHAZB_002663"
     CDS             161..1270
                     /gene="pdhA"
                     /locus_tag="ACHAZB_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HFM8615277.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            1273..2250
                     /locus_tag="ACHAZB_002664"
     CDS             1273..2250
                     /locus_tag="ACHAZB_002664"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HFM8615278.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
BASE COUNT          749 a          442 c          493 g          610 t
ORIGIN      
        1 atttcctttc atcgtgaaaa tttgtgcttt tagcttgtat ttctcataaa tccatgataa
       61 tttattaatg aagacggtta aatagtgata cagttttatt cactgtttca atcgcagaat
      121 gaagagattt tcttatagat gagagaggaa tgtgtgacaa atggcaaaca aaaaagcacc
      181 gatcgacttc caagcactgc tagatgaagt caatgcagat ttcccagtat atcaagcact
      241 tgatcaagat ggaaacgtag ttaacaaaga tttagtacct gatttatccg atgatgaact
      301 cgttgaatta atgactcata tggtatggtc tcgtgtatta gaccaacgtt caacagcgct
      361 gaaccgccaa ggacgcctag gcttttttgc accaactgct ggacaagagg caagtcagct
      421 ggctagtgct tacgcatttg ataaagaaga tgttcttctt ccaggttatc gggatgtacc
      481 tcaactagta aaacatggtt tacctttatc tcaagctttt ttatggtctc gaggacatgc
      541 tgcaggtaat tcttatccgg aagaattgaa agctctacca ccacaaatta tcatcggtgc
      601 acaatatgta caagcagccg gtgttgcatt aggtttgaag aaacgaaaca agaaaaatgt
      661 cgtcttcact tatactggtg atggcggttc atcacaaggt gatttttacg aagcaatcaa
      721 tttcgctggc gcttaccatg caaatgccgt attttatatc caaaacaacg ggtatgcgat
      781 ctctacacct cgtgaaaaac aaactgcagc aaaaacactt gcacaaaaag ctgtcgcagc
      841 tgggattcct ggtattcaag tcgatggaat ggatccgctt gcagtatata ctgtatctaa
      901 aatggcacgg gaatgggcga tcagtggaaa tggaccagtc ctaatcgaaa cactgactta
      961 tcgttatgga ccacatacac tatctggtga cgatccaacc cgctaccgta caaaagacac
     1021 agatgacgat tggcagaaaa aagatccatt gatccgcttc cgtaaatatt tgacggaaaa
     1081 aggactttgg tctgaagaaa aagaagaaca agtcatcgaa gcaactaaag aagaaatcaa
     1141 agcagctatt gctgaagccg acaaagtacc aaaacaaaag gtttcagatt tcttgaaaaa
     1201 catgttcgaa gtatctccac aatcaatcaa agaacaaatt gcaatttatg aagcgaagga
     1261 gtcgaaataa tcatggcaca aaaaacaatg atccaagcaa tcacagatgc cttagcactt
     1321 gaactagaaa atgacgagaa tgtcgttgtt tttggtgaag atgttgggaa aaacggagga
     1381 gttttccggg caactgaagg attgcaggaa aagttcggag aagaccgagt attcgatact
     1441 cctttagctg aatctggaat cgcaggattg agtttcggtc ttgcgttaga aggatttcgt
     1501 ccagttcctg aaatccaatt ctttggtttt attttcgaag caatggacga agtagtagct
     1561 caaatggctc gtacaagata tcgaatgagc ggcactagaa atctacccat cacaattcgt
     1621 tcgccatttg gtggcggtgt tcatacacca gaattgcatt cggataattt agaaggtttg
     1681 atcgctcagt ctcctggtat tcgagtagtg attccttcca acccttatga tgcaaaagga
     1741 ctattgattt cttctattag aagtaacgat ccggttgttt tcctagaaca catgaaacta
     1801 tatcgctcat tccgagaaga agtgccggat gaagcttacg aagttccttt ggataaagca
     1861 gcagttactc gtgagggaac agatgtatct attatcacat acggagcaat ggtgcgtgaa
     1921 gcaatcaaag cagcagacaa tcttgcaaaa gaaaatatat ctgttgaaat cattgatctt
     1981 cgtacagttg cgccattaga tgtagaaacg atcattcaat ccgtggaaaa aactggtcgc
     2041 gtagttgttg tccaagaagc acaacgacaa gcgggcgttg ccgcgcaagt agtttctgag
     2101 atctcagaac gcgctatcct ttcattagaa gccccaatcg gacgagtttc tgcacctgat
     2161 acagtcttcc catttggaca agcagaaaat gtctggttgc caaatgcaaa agatatcgaa
     2221 gacaaagtaa aagaaatcgc ggaattttaa gaaaagaagg gacgaaacaa agtcatggct
     2281 tatcaattta aatt
//
LOCUS       DAYYCY010000232         2290 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.237, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000232 DAYYCY010000000
VERSION     DAYYCY010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2290
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.237"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            316..570
                     /locus_tag="ACHAZB_002665"
     CDS             316..570
                     /locus_tag="ACHAZB_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFM8615279.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEDNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            571..888
                     /locus_tag="ACHAZB_002666"
     CDS             571..888
                     /locus_tag="ACHAZB_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFM8615280.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            complement(1522..>2290)
                     /locus_tag="ACHAZB_002667"
     CDS             complement(1522..>2290)
                     /locus_tag="ACHAZB_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
                     /protein_id="HFM8615281.1"
                     /translation="RDTVIIACVIWGIINGYTSQRATYRAVCSVLFLNGDFPSRSRFT
                     RLSSNLAYTIKIIRYFFIKKLTKGELVGIIDSFPSQLCKPVRNRQAKLLNQIAKVGYN
                     STKKSYFYGLKIHMIVTKTGFPITYSITNPGVHDVKVLETLSEEANLPNILGDKGYIS
                     HKIHEKLALKGITISVPPRKNMDKSEKLDHSLLGKQRKTVETVFSSLEKLGCQNFNSR
                     SVKGLESRFESILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT          770 a          372 c          430 g          718 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaatggatgt taacggaagt gtatggtgag tagttcactc gtattttgat atcgtttgta
      181 tatgttttca gcgttctaag tggcttattt tgtccaataa taatatggag tggaatccct
      241 taagaaacac cgttggcaat tgttgttata acatgttata atttattcat caaaaactaa
      301 aaggagtgtg ataacatggg aaaagaacta aaggttagaa agatcggcaa tagcgtggga
      361 gtgatccttc caagctctct tggattaaaa agcggagaca ttatccaagc caaacaagaa
      421 gacaacttat tcatattaga tactactcaa atagcaaaag aacacgatcg caaattgatt
      481 gaagaaagtt tccaagactt tgagaaaggt ttaacagttt cagaaattga aatggtaaaa
      541 gctttcggaa aatacggctg gagtgaatag atgaaagagt acacgctagt ttattctaaa
      601 agttttcaga ccagccttga aacgataata gaagaatggg aaaatgagct tgctctttct
      661 ccagccagca taaaaagatt tgtaggtgcg atccagaagg ctttagaact aaccaaaaat
      721 tttccaacta tgcatgagga agtttccgaa atctatggaa tggatatccc aacatatcgg
      781 atcgtgatcg gcaaaagcta tgccttattt tatcggatcg accatgaaaa aaaagagata
      841 cttgtaggac gtattttcca atctaaacaa atgaaactag gcttttaaca ataattagga
      901 aaaagtcgct catcctctac aggtgggtga ttaatgccgc tcgatatggt tgttaaccgt
      961 cggttactcg aagattgata tataagactt agtacctttt tattttgctc catgttatgg
     1021 ccgaaggtct aaagatacaa tcattagtct agagaaaaat taagaacctt aggaactacg
     1081 gggatcgctt ggtgaataag agacactgct attagtgaaa aaataagata tcaagtacgc
     1141 tctattccca aaaatttcgt gactttactc atgagaggtt caagtagtat atttttaatc
     1201 aaaattgacg taacttttaa taaatactac ctaaataagg tagtatttat ttttgtgttt
     1261 taaaagagga agtccaatat atctatggga aacttcttaa atattatgtt ggtacatgaa
     1321 aatatacttc cctgctagac tatttacaac attttacttt gtacgtattc ttgttttaaa
     1381 aggaatggga taaaatatag gtacaataac tatttgatgc taaatataat taatggagga
     1441 gagatagtga acatacttaa tgaaaaaaga tatgtatggt ttgctggtaa aaaatatatt
     1501 aacccgtggt gctagttaat tttaatatcc taaagaatat ctcaaagttc cttcaaaacg
     1561 tcgttgtgct cgacttaata gaacactgta agccagtaat atgctttcaa atctactctc
     1621 tagcccttta acagaacgtg agttgaaatt ttgacaacct aatttttcta aggaagaaaa
     1681 aacagtctca actgtttttc tttgttttcc aagtaagctg tggtctagtt tttctgattt
     1741 atccatgttt ttacgtggcg gaacggatat agtaatgccc ttaagggcaa gcttttcatg
     1801 gattttatgg ctaatatatc ctttgtcccc taggatattg ggaagattcg cttcttccga
     1861 taaagtctct aacactttca cgtcatggac acctggattc gtgattgagt aagtaatcgg
     1921 aaagccagtc ttagtaacga tcatatgaat tttaagacca taaaaataag atttttttgt
     1981 tgagttatag ccaactttgg ctatttgatt caatagtttt gcttgtctat ttctaactgg
     2041 tttacataat tgactaggaa aactatctat aattccgact agttcacctt tggtgagctt
     2101 cttgatgaaa aagtatcgaa taatcttgat agtgtaagct aagtttgaac ttaagcgagt
     2161 gaatcgactc ctactaggaa agtcaccatt aagaaataag acggaacaaa ccgctctata
     2221 cgtggctctt tggctagtat agccattaat tatgccccat ataacacaag caataatcac
     2281 tgtatcgcgt
//
LOCUS       DAYYCY010000233         2283 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.238, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000233 DAYYCY010000000
VERSION     DAYYCY010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.238"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            73..591
                     /locus_tag="ACHAZB_002668"
     CDS             73..591
                     /locus_tag="ACHAZB_002668"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFM8615282.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            719..1402
                     /locus_tag="ACHAZB_002669"
     CDS             719..1402
                     /locus_tag="ACHAZB_002669"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HFM8615283.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1711..1784
                     /locus_tag="ACHAZB_002670"
     tRNA            1711..1784
                     /locus_tag="ACHAZB_002670"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1745..1747,aa:Arg,seq:tct)
     gene            1943..>2283
                     /locus_tag="ACHAZB_002671"
     CDS             1943..>2283
                     /locus_tag="ACHAZB_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HFM8615284.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLA"
BASE COUNT          768 a          349 c          462 g          704 t
ORIGIN      
        1 gctataattt gaaaaaaatt tatcgttatt atatgattaa gaaggattga ataaagaaag
       61 aagttgaaaa cgatacctga ttacaaaaaa ataactttta taagttctat aacgttgatt
      121 atatttttaa ttcttattct tctcgtcgtc ttggttcccc gaatttttgg tttgactcct
      181 tatattattg aggacaaaga aatgagtcca tattatgcac aaggaaatct gatatatgta
      241 cgaccgaaaa atacaaatga gattttagtc ggagatatta ttacatatta tgaaaattca
      301 ggaaaaaaaa tctcgacgcg tcgcgtaata gccgttgaaa acaaccacga agatttttat
      361 acaaaagctg atacaaagac ttatattgag cctggagtag taaaaaagag aaatattatc
      421 ggtagtccca tctttcagat tccatatatt ggactagtcc tttcaaaaac ggcatttgca
      481 tggattcagg ggttattctt tacgattgct ttttgcctaa caggaatcac agcatggaat
      541 acctttgtag agttgaaaaa gaagcggaga cgaacacaga aatttttgta agaatgttaa
      601 aaacataaat gtattccaga gatccaacga ctcttctgaa catgaaacac gagaaaacac
      661 tcatgcagct tgtagattta gtcagttgtt cgtataatgt gtgaagagga ggagtagtat
      721 gcgcaatgaa atgatgcatc gtcctgtagt caaagaagaa ctattggatt ttatgcgtac
      781 ccagcaaaaa aaattaccgg gtgagctagg caaactggaa gaagaagctc atgtagcaga
      841 agtaccagtt attccccatg aaacagttgt atttttgaaa tttttacttg gacagcttca
      901 gcctgaacga atcttggaga tcggagcagc tatcggtttt tcatcaagtg tcatggccac
      961 gacaatcgga gaaaacgctc atgttacgac aattgatcgt ttcgatgtca tgatcgagaa
     1021 agctaaaaaa aactatgaac gtctaggact gacagataaa gtgactttgc ttgaaggtca
     1081 agcagcagat atcttgccgg agttgtctgg tccgtatgac tttattttta tggatagtgc
     1141 aaaatcaaaa tatattgaat ttttacctga atgcttacgt ttgttaagaa aaggtggcgt
     1201 tttgatggta gatgacatct tccaaggagg aacgatcttg ctcccagatg aagagattcc
     1261 ccgtggcaaa cgagctatcc atcgtaaatt aaatgaattt cttcgtgttg tgatggatca
     1321 tcccgatttg acatcgacaa tcttgccatt aggtgatggt gtgatcttaa tgacaaagga
     1381 atcagaaaca atcgagttgt gatacatgat tatggcagcc tgtagataat cgattgaatc
     1441 gagggctcaa ttcgaacttg atgtaaaagt ttctatattt gtaagtcaat taaaggatac
     1501 aggaaatcta aataaacagg agtagcatga agtcaatgta gctcctgttt tcaattgata
     1561 accttagaac agctgaagga gaatgtctcg ttttaaaaga ttgatttagt agttgggcca
     1621 aaataattca ttatttgcta attaaataac tatttcgtat tgacagaagc aatttaatta
     1681 tctataatta ttgatgttcc gctgattctt gtcatagtag ctcagctgga tagagcatcc
     1741 gccttctaag cggacggtcg ggggttcgaa tccctcctgt gacgctaaaa aaccgttaga
     1801 aactgaggtt tctaacggtt tttctttgtt ttttgtgcca atattgtttg ttttcaaagg
     1861 caaataaaat aaaaaaacat ccttatcgag tacaattata aggagcaaaa aaataaaact
     1921 catcagaaaa gaggaagaag atatgacgat actatctaga attttagtga ctctagttgc
     1981 aattgaattt ttttatatca tgtatatcga aacggtgaga actgattctg acacgacaag
     2041 tagggttttt aaaatgagta aagaagagtt aagcagaaaa tctgttcaaa cattgtttaa
     2101 gaaccaagga gtgtacaatg gactattagg agtgggacta ctttatggag cttatctttc
     2161 ttccgcatct aaagaaatca caagtatgct attaatatct attttttttg ttgcattata
     2221 tggcagtttg actagcgatc gttggatttt actcaaacaa ggtgggctag ctctgttggc
     2281 att
//
LOCUS       DAYYCY010000234         2264 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.240, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000234 DAYYCY010000000
VERSION     DAYYCY010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.240"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..277)
                     /locus_tag="ACHAZB_002672"
     CDS             complement(<1..277)
                     /locus_tag="ACHAZB_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HFM8615285.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCK"
     gene            complement(778..1092)
                     /locus_tag="ACHAZB_002673"
     CDS             complement(778..1092)
                     /locus_tag="ACHAZB_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8615286.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
     gene            1378..1617
                     /locus_tag="ACHAZB_002674"
     CDS             1378..1617
                     /locus_tag="ACHAZB_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8615287.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            complement(1979..2239)
                     /locus_tag="ACHAZB_002675"
     CDS             complement(1979..2239)
                     /locus_tag="ACHAZB_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8615288.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
BASE COUNT          772 a          389 c          327 g          776 t
ORIGIN      
        1 acttacattt aaatcgttgt ttcttcagct ccagataagt gcgatactca gacacatcat
       61 tcagtaacaa ccgaacggtt gtcgtccccc aacgaatgat cctagagggg acgacacaaa
      121 gacaatggga acactcttct ggcttgtaca gaagagttcc tttgtaaatc aagcaaattt
      181 gccctttgta tttttcttta gttaaacaat tttcctcgaa atagatattt aggtctagaa
      241 tatttagggt atctttgata agctgagtat gagacatctt gaatcctcct atgaattggt
      301 agccaaatta attctaaagg atttcagggt gtcttttcta tattttttta tacaaaaaag
      361 atgttggtga agttcaccaa catcaaaaag tatagagcct ttattttggt caaggagaga
      421 aaagtagaga aatacaaaat tttttgcatt gattttgcat tcaaaaaaac attcaaacat
      481 ttagaaatcc ccaatacgta ttcaggaaaa tctattttaa ggtttacaaa aagaactttt
      541 gttcgtacac tctttttgag gtgaactttt atgctgatgg aaggaaaaac acaattatgg
      601 tttaagcatg ttccttcaaa taagtttttt aattataaac tctagagaaa ttaattcatc
      661 ctaacataaa ataattgttc actcaaaagt ttttctagaa caattaaaac agcaggcttc
      721 acattcgcta tacatttgta agaagtaata attcctgtac taaaaggcat aattgcttta
      781 attatttttt gacttctctt caaacacaag aagtagttgt gtaataattg cagaaataag
      841 gattccaata atagatataa aatttgacat ggaaacgtct ataacattaa aagaataaag
      901 tgttgaagaa agtaaactga ttgaaataac ccctccgtaa aaaatagtct gtgaatatac
      961 ccactttttt tcagaagacg tacttcgttt ggttctaaaa cctatccctg catttctact
     1021 cttatcaaaa aaccaaatat ttagaagtaa taatattgct attattaaga taaaattact
     1081 gtaaaaatcc aaaagctatc ctcctagcaa ctatttaaat acccaaaaaa attacctcta
     1141 gataatatca tacctacgtt ttcacttata aattttttca taaaaaaagc gtttagaggc
     1201 ataatcgtta atacagatgg taaccaatgt atctggtttg aactgggata tcaccctctc
     1261 catatatgta ttactaagac taatagatac tataaaagac ttgatagttt tgcattgatt
     1321 ttgcctaata ctgagcatag gtgtatgcta aacgatacta taaggagtgt gtacataatg
     1381 aaaagaattc tgtttatcag tttatcaaat ttattgttac ttactgcttg tgggacttct
     1441 gcatcaaaga acataccttc agatacgcaa atttcgaata gtatcgattc aacaatttct
     1501 acaaatgaga aaaattctcc ggtagaaact tccgatataa aagtttctac ctctgcaaaa
     1561 aacagggtaa agaaactgtc agcacgtctc ctttatttca aacaaactct gaattagctt
     1621 cttcatcttc ttcagcaaat aatgatgata gtttcgcgtt tactcctaac taaatgcaac
     1681 ctttacaaat gctttacgat aacaagcctg agctaaatga tcctgatatc agttttgcat
     1741 tttttgaaat gattggatcg gatgatcttt ttaaaactac tagtttgtct atccgaaaat
     1801 aggacagttc tggcagtata ggtttttatc gagtttctcc accaggcctc gttacagaga
     1861 cagattctct aggcgaacct tttgaataaa tcaaaaatca tgaacaatca ctgccaacta
     1921 aataaagtgc cttcaaaagc ttggtcttct cgcttttgaa ggcactttat ttaaatcttt
     1981 atgaaactaa agtatgttca tatttgttgc tatagttata tagatattct gttgtttcca
     2041 actcataaac gtgagtatat tttgccacta cttttccgca tttttctaat atttcattgc
     2101 gatcgacgtc atgatattcg tctacgcaga tcacaacaca attttctaat ttactgatta
     2161 cttctccaac cactttttgt ttcaagccaa taggttggca aatatatttt tttcctaaaa
     2221 tgatagtttc gttattcata tttcatccat ccttaatgtt tttt
//
LOCUS       DAYYCY010000235         2256 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.241, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000235 DAYYCY010000000
VERSION     DAYYCY010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.241"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            63..1925
                     /locus_tag="ACHAZB_002676"
     CDS             63..1925
                     /locus_tag="ACHAZB_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM8615289.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            1925..>2256
                     /locus_tag="ACHAZB_002677"
     CDS             1925..>2256
                     /locus_tag="ACHAZB_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM8615290.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          810 a          337 c          445 g          664 t
ORIGIN      
        1 aacatttcat attgtcgtta gtttagaaaa cgcttatatt agcaatgtaa ggagagatga
       61 taatgcagca gctaacaaaa agagaaaggc agcttttgat ttacttggtg gaaatgtctg
      121 aaccggtaac aatcaaaaag ttggctgaaa aagaaaatgt tagtgttcgg acgataaaat
      181 acgatctaga tgatataaga gagtggctga atgagcggaa gcaagtccta tatagcaaac
      241 gtagtcaggg aatttggatg aatattagta gctctgatcg aatcaaatta aaaagcgaat
      301 tgatggatgt taatcgtttt gaaatatttg cagatcaaag tctccgaatc aaacgtttac
      361 taattatgct tttagtaaca aaagaagcta ttactggaat gaaaatggcc aacgcattag
      421 atgtcagcaa agatacgatc atgaatgatt tagatgtgct ggaaaataat tttcttaagg
      481 aaggattgac cttgaatcgt caggcgcgaa agggtttttg gatcatagga gaagaacgtc
      541 tagttcgatt aacgattgaa gagattcttc aaaaagagtt cacggattat gacatttata
      601 aattaatgtc attgttattg aatggcggtg agactgaata ctttgaaatg tattctgcta
      661 ctgctacacc tatccaagag gtatttaatc aagtaatcat tcgaatgcga cacttactgg
      721 aatttgaaaa tctagagaaa ctgaattacg cggaattact aaatattctg attcgagtga
      781 caattgctac cgttcgctta cgcaaagagg ctacgattgg tcgttatcaa ttggttgctg
      841 aacaagaatt agacaaaaac gatctttctt atcgcgtcat gcagcaggtc tttgattatt
      901 atcagttgcc actgttcgag gatgaatatc attatatcta tagtgataca ttcgacacga
      961 ttcaccagca agacgtcatg aaactagttg agaatgtgat ttataaagta agtaaagcaa
     1021 tcaaccattc tttttatcaa gatccacaat tgttgacgaa tctgtatgct catttatcca
     1081 tgcgattatc gagaaagaaa aaatttgtca atgaatataa tccttttaaa gaagatatca
     1141 agcagaaata cccggaatta tttgatgcga tcaagcaagc agttcgacaa gaaataagtg
     1201 aacaaaatat tctgatcaat gattctttcg tggcatatat tgccttacac tttttggttt
     1261 cctacgaaag agaagaagat ctacgaagcg ttagagccgt ctacatttgt tcaacagggt
     1321 tgggtgtgac tagtctgatc aagcaaaaga tcaatgaaga attaaataat atcactatcg
     1381 tggcatttgc ttcagtattg aatgcgcaag aaatgattgc taccaaagat cctgatttgg
     1441 tgattagtat ttttccaatt aatggcatcg atcgtccttt catcaaagtc catccattac
     1501 ccactgaaca ggacttgaaa aaaattcaac aaataataaa aaaaatactt gcttcttcca
     1561 atcagcagga aaggcataac ttgaatctta gagaaacgtc catcaataaa gatactttag
     1621 aagactttag caaggaattg attttgaaag cttatacgat ttatgaacgt ttacttgtca
     1681 ttttggaagc agaattgatc caagaatatc gagaagcttt tttgcttcat gtattcttaa
     1741 tggttcatcg tatcacattt gatcaacaat atatggcaga aggtcgtcaa gtagaccaga
     1801 ttattttaga caaaggaaca ctggtaggaa aaatcgaagc agtttttgca gaaaataatt
     1861 taggaatcaa tcagtcagaa atcagtgcat tattctcata tataaaagaa ggagcgtcaa
     1921 gttagtgaaa atgtcagacg aggctttaaa aattatcgag gaaagtcaaa atcgggaaaa
     1981 gttaaaacaa gtgattgatc taattgaaga gcgattagta gaacatgcta ttgtaccaac
     2041 cgagttgcag tggaccattc taattaatca cttgaatgag atgctgaaac gttctcaaag
     2101 aaaggaaacg attcccgtag tcgatcggga tttgttcaaa gaagtatcca aagaagccat
     2161 aactatttct gatgaaatag ttagaagtat cggtaattta accgatgatg aaatatatgt
     2221 tttgtcgatt cattttgaaa cagcaaaaaa taatta
//
LOCUS       DAYYCY010000236         2255 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.242, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000236 DAYYCY010000000
VERSION     DAYYCY010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.242"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..510
                     /locus_tag="ACHAZB_002678"
     CDS             <1..510
                     /locus_tag="ACHAZB_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8615291.1"
                     /translation="EIINFRLKTKKESVIILVYFQGGNLMIKKLFLLSILTLAMAGCS
                     DSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGILGRSNCKHRCSNRRRCRRS
                     NRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREVGDEPKLEYAFSFAREDED
                     NPKTYDILA"
     gene            complement(571..936)
                     /locus_tag="ACHAZB_002679"
     CDS             complement(571..936)
                     /locus_tag="ACHAZB_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02328 family protein"
                     /protein_id="HFM8615292.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(1012..1257)
                     /locus_tag="ACHAZB_002680"
     CDS             complement(1012..1257)
                     /locus_tag="ACHAZB_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8615293.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(1327..1581)
                     /locus_tag="ACHAZB_002681"
     CDS             complement(1327..1581)
                     /locus_tag="ACHAZB_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8615294.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(1745..2068)
                     /locus_tag="ACHAZB_002682"
     CDS             complement(1745..2068)
                     /locus_tag="ACHAZB_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8615295.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
BASE COUNT          853 a          371 c          390 g          641 t
ORIGIN      
        1 gaaattatta attttcgtct gaaaacaaaa aaagaaagcg ttattatttt agtctatttt
       61 caggggggga accttatgat aaaaaaacta tttttactat caatattaac gctagcaatg
      121 gcaggttgtt ctgactctgg cttagcaact gaactgatca caaaatcaac tgaagccacg
      181 acagaggagt taaaatgtgg aaacgatcac agtgactaca gaggcttaaa tcgacgatgc
      241 atgggaatat taggcagaag caattgtaag catcgatgta gtaatcgaag aaggtgtaga
      301 agaagcaaca gaaaaatcaa aagaaagatc aaaagaagca atctgtgctg tgcatcgtat
      361 acagacgtcg aagatgcggt acttttaaac cacgtcagga gatgttcatt tatgtctagg
      421 gaagttggcg atgaaccaaa actagagtac gcttttagtt ttgcacgtga ggatgaagat
      481 aaccctaaaa cttacgacat tttagcttga tctaagatgg ttaaagcttt ttaatgaagt
      541 aagaggagat agaaggcttt tctagttata ttaggtaaga taaattcctt tttgctccaa
      601 atttgctaaa cattctgcta gataaacttc atcgtgttcc ggataaatcg gcacgttgcg
      661 ctctacagta ggtaaatcaa aaaatgcttc gcatgatttg ccacgataca taggattttt
      721 ccactctggt gctgggctgt aaccacgttt ttccatttca tccatgacaa gctgatgata
      781 ttggaataat ctataaggag aatagtgaaa aacataatcc acagtagcat gtttctttcc
      841 ccagccattt cctcttaatg cacagcattc ccgatgttgt cctaaaagct gttgacgagg
      901 aagctttgaa atcaattttt catgccataa tcgcatagaa atgtctcctt tatatataga
      961 ttgttaacct tagacgttaa atgaaaaaac cagcaccaag gctggaaaaa atcacttcag
     1021 tttgcttgct gtttctgagc gaccaattag gtaatcaacg gaaatatcgt aaaagtcagc
     1081 gatatagatt aattcaatga tgcttggaat ttcttctcct tcttcgtact tacgatattt
     1141 gaataaagga atacttaacg cgctagaaac agctacttga gataaagatc ggccagttct
     1201 taagaaattc agacgacaac gtaaagtatc cagtaaaata gcttcttcga tactcattta
     1261 atcacctttc attatagtag aatagttata atgactatag aaagctacac atgcaatcaa
     1321 tagtactcaa tgttttttat ttaaacgtat caaataaaga atgacgatca ataaagacaa
     1381 aaccagtcct gataattctt ctataaaaac actatcgggt tcaaagtagt tgtaattaaa
     1441 aactttccac ataaagtaga tagccggaag agtcaaaata gctaaataaa accgttcttt
     1501 gttccaattc ataagataaa caatacttgc gaccagtgcg accgcaggca cccaaaacaa
     1561 aactctcacc aacgtaatca tgtaacggac tcctcaacga ttcatataat aaaagataac
     1621 atgttattgc atacatcaca aatgaaatgc taggaaaata aaagaagaat agatcaatga
     1681 aaaatgaaaa agcagaacag ttttctgctg ataagcagga actgttccgc tgaatgtgtc
     1741 ttatctatcg agaaaaaatc aatggattga tatctaagta attcaataca tataaaaaga
     1801 ggacgatcgc attcactaaa aaggaaagaa tgatgatccc atagcgaatg gaaacatatt
     1861 taacaaatac ttttgtttga atacgggcat atctttttcc tttatagctg agcatcatga
     1921 caagaagcaa aagggtaatc acaccttgaa aaatcattcc aataaacaga attcctgagg
     1981 tttgataatt taattggaga aaataagctg ctgccgcaac taaatcgaat aaaatcgcaa
     2041 taaatggcaa aataatcact cctaacattg agaataaata aaagaataac attttttcta
     2101 aaataagcaa ataaaaatta aagtaacaaa atagttatga cacaagtaaa agaaataaga
     2161 ataacaatat tattttactt atattttgaa acttgtgaga gtttgtcatt cgtgctatag
     2221 ttgaaaagta acaaaaataa attttattta tgatg
//
LOCUS       DAYYCY010000237         2225 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.243, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000237 DAYYCY010000000
VERSION     DAYYCY010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2225
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.243"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..209)
                     /locus_tag="ACHAZB_002683"
     CDS             complement(<1..209)
                     /locus_tag="ACHAZB_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8615296.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIEL"
     gene            302..651
                     /locus_tag="ACHAZB_002684"
                     /pseudo
     CDS             302..651
                     /locus_tag="ACHAZB_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(923..1213)
                     /locus_tag="ACHAZB_002685"
     CDS             complement(923..1213)
                     /locus_tag="ACHAZB_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8615297.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1421..2113)
                     /locus_tag="ACHAZB_002686"
     CDS             complement(1421..2113)
                     /locus_tag="ACHAZB_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFM8615298.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQALTDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          626 a          423 c          327 g          849 t
ORIGIN      
        1 agttctataa taattcttga actccatgtc ttataatgtt ctatcgaagc acccaaatag
       61 ccaagttcat ctaatctact tgcataaact acgtcatccg ctctcatagt cggcatatac
      121 atattaaaga gtaaaaagat accccaaatc cctattagta acaagatcag ttctttgtta
      181 ttcgttattt ttctattatg tttgttcatt tttatactcc ttcttatatt tcctaataga
      241 gtataacata gctttaagca ttacttaaag ataacttaaa agttatcttt taaatactta
      301 attgttaaat accttatttg atgcaaaaga aatctacgat agtcaacaaa aaaattttca
      361 ttatatccag aaaaattttt cttagttaag gacctatgga ttgcagtaat gcagaattcg
      421 tcgaataaac tgccaaagac atacaatcat attgctttta aaattgacga acaagatata
      481 gattcgtttg tctcaaaaat ccaaatgtta ggtttaactg tcgaaccagg acgttctcgt
      541 gtgaaaggcg aggcttcttc aatttacttt tacgactatg ataatcacct cttcgagtta
      601 cataccggta ctctccagga acgtttaaaa tcctacaatt ccactacctg agactagtaa
      661 tgactagtct ctttttatgg cctctgatcc ccccattgag cgaatactaa tagggcttcg
      721 aattgatctg ttcaatcttt aagcaatatc cctcaaatga gtttggaaag tttagtgatt
      781 catcacaaat acttaaatac atgtagttat atatagattg aggttcttgt gagcgtatgc
      841 tcgtgtgggt tcaaatccca tcgtctgcat ccatctttcc aaaacaagcg gctaggacat
      901 ttttgtctca gccgcttgtt ttctagttta ttctttcatc ttgtcggacc ttttttcttg
      961 ggtatagact tttctcatat tctaccacaa ttttgggttt ccctaacaca tgataggatc
     1021 tcacaacaaa ttgctttcta ttattataat aacctgtaac gactaacttc atcccttcgt
     1081 ctacttcagc taggaagttc aaactatggg aagcaatcaa acaattgacc ccatttaaac
     1141 tgaaacggat caacggtctg gcactaactt gcaaagtacg gatcttatct actgttccat
     1201 attttttctc cataaccttc acctcaatac cattatacga acatacattc cctttgtaaa
     1261 gaagaaactc tgttttatct ttttgacttt ttgtctatta cctttcttaa aatcatattg
     1321 gcacttcctg cgaagatgaa ggacatgtac taaaaaaaag aggcagcagt atagattact
     1381 gttgcttctt tactaattta ggtttacttg ttaagaaaga ttatttacgc aaatgttctt
     1441 caacaatttg ctgagattta tcagctgtat cttccacttc atcttttgct ttttctaact
     1501 tttgaatata ttgtttcaag cttgagtttt cagcagttgc ttcgtctaat ttcttttgtg
     1561 tatctgttaa ctgagtttct acatttgttt tttcttgtgt cagtgttgcg atttgttgat
     1621 tcaaaccatt tacagtattt tgtaattctg aaagttttgc ttgtgcttct tgtagtcttg
     1681 ctgtccaatc tgcattcaga tgattgattt catcattttt ttggttgatc tcatctagtt
     1741 tgacattgat ttcattttgt ttgctttgta gttgtgcatc cttgctgact aattgacttt
     1801 ctaagttttg tttttctgct gccgattgat ttttttggtt ttctaattca tttacttttg
     1861 tttggtattc ttggacatct gcctttgaat cttgcaatgt ctgttctttt tgtgctagtc
     1921 gagcggtaag atctgtaagc gcttgctctt tatctgtcaa tgtatgatca agctgtgtca
     1981 gattctcatt gattgtatca atatctgtct ctccacccca caaaataatt tcatttgcgt
     2041 accgcgtgcc tgtgtagcca attagacata gaaatgcaac gacaatccca ccagtaataa
     2101 atttcttttt catttgcata gctccttaat ccaatacttt tacatgctaa aagtaagtga
     2161 tctcaaacac aatgtcaagc gtttttaaat tacatgtgta attttaatat aaatactatc
     2221 aaata
//
LOCUS       DAYYCY010000238         2218 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.244, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000238 DAYYCY010000000
VERSION     DAYYCY010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.244"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..365
                     /locus_tag="ACHAZB_002687"
     CDS             <1..365
                     /locus_tag="ACHAZB_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM8615299.1"
                     /translation="CVIQTLNKAKIHYHIPEGMILHTDLGSQYTAREVEQWLKTNKIR
                     HSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEEANRALFSYIEGFYNRNRIHSS
                     IHYLTPQEFEELAKEKMA"
     gene            complement(721..1293)
                     /locus_tag="ACHAZB_002688"
     CDS             complement(721..1293)
                     /locus_tag="ACHAZB_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8615300.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENCVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(1309..1914)
                     /gene="fic"
                     /locus_tag="ACHAZB_002689"
     CDS             complement(1309..1914)
                     /gene="fic"
                     /locus_tag="ACHAZB_002689"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="HFM8615301.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
     gene            complement(2112..>2218)
                     /locus_tag="ACHAZB_002690"
     CDS             complement(2112..>2218)
                     /locus_tag="ACHAZB_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFM8615302.1"
                     /translation="MEAIRGLYKKTRKEGTLFGFSVCTEIKVLLGIPA"
BASE COUNT          685 a          457 c          337 g          739 t
ORIGIN      
        1 attgtgtcat tcaaacattg aataaagcaa aaatacatta tcacattcca gaaggaatga
       61 ttctacacac tgacttgggc agccaataca cagcaagaga agtggaacaa tggcttaaaa
      121 ccaacaaaat aaggcattct tatagtagaa aaggaacacc ttatgataat gctggaatcg
      181 aatctttcca tgcctcattg aaaaaagaag aagtctacac gactagctac tcagattttg
      241 aagaagcaaa tcgagcgcta tttagctaca ttgaaggatt ttataaccga aatcgaatcc
      301 atagctcgat tcactatcta accccacagg aatttgaaga attagcaaaa gaaaaaatgg
      361 cttaaccgtc tctactaaag acaatcaaca ccgtagggat atggagataa aatcaataga
      421 gtcaatagat tatttatcga taataacact aaaaaagcca cgttcctaaa cgtgacttac
      481 gctgaacgat atgtgttgtt tggtagacga acaaatcgtt cattataaat atatgatggt
      541 atcctaatat agtaagcgtg gctagcatac ctttaattac tttacctttt acagttacca
      601 tacacatttt ttgagcagaa atcaagtaaa taaaaaaata ttatatttca caacatgcta
      661 atagactcat atctagaacg cattaagtat ttacctgtct ggttcgctga gacttctttt
      721 ttaactttct tcaattttct tctttattct aaaaagcgta ctgcgactga aaccggttat
      781 agcttcaact tccttatacg atttacctga agtaagcagt tcatatgcgt gcctcttctt
      841 cggtgtgatt gttgctttcg gacgtccttc tctgaaatta ggattattct ttcttgcaaa
      901 catttttcct tcttgggtac gttccacaat catatcacgt tccatttctg ctacactcaa
      961 caacgtccgt gtcaccattc gccccatgga cgtattttca atcgttccta gattcaaaac
     1021 cttgatcgtt acgttatcat cgagtaaagg ttcaatgatc tctaatgctt ctctcgtgtt
     1081 acgagcaaat cgatcaagct tcgtaacaac tatttcatct ccaggcttca gaccagccaa
     1141 cagctgattg aattttggcc gatcccgttt tgtacccgta aatttttctg aaaaaatatt
     1201 cttcttctct acgcagttct caagcaactg ttgaatttgt tcttttgtac tttgagaagc
     1261 ggttgagaca cgtgcatatc catatttcgt catttggtcc acctactttt acaaatcatc
     1321 tatcgtatac tcagaatacc cttcatagaa atagctgaca tcgattcctt tcatatataa
     1381 ctcgcgatcg tcaattttat cagtcagagc attggaaatt agatagcgta tttccaaatc
     1441 gtttatggga cttcgctcca ttgcagaaag atagtctgct ttatcgatta agttccaatc
     1501 tacaactttt tgcaattctt cctttaaaat gagatccagc caaattctag tacttcggcc
     1561 atttccttct cgaaatgggt gagcgatatt catctccaca tatttcttga taatctcttc
     1621 aaaagaagtt tgtggcatct gatcaatatg ctgtaacgaa tgttctaaat acataactgg
     1681 tgcaaatcga aaatttcctt ttgcaatatt gaccgatcgg atttttccgg caaaatcgta
     1741 aatatccgaa aatagaaatt catgaatttc agataaacct ttaaatgtac caacttcgat
     1801 ttcatttatt tttccacaat cgtacaattc tttcgctttt tgtttactga gtttttcttc
     1861 aaccttagcc aactcaactt gattggttaa tcccaattta ttctctaaaa tcatgcgaaa
     1921 tctccttaac tttatgacat aagtttatga aacactctca accccattat atcgcgattt
     1981 tgtttatgtg tcaaaaacta ttaagtttct gacccccgat agatttttca agtgtctcta
     2041 ttttgcacag tctagttggt tctgttgcaa agttttaaat aaagaataaa atcccttacg
     2101 gtatctatga tttaagctgg gattcccaat aataccttga tttcagtaca gaccgaaaac
     2161 ccgaagagag tgccttcttt tcgggttttc ttatataatc ctcgaatggc ttccatgc
//
LOCUS       DAYYCY010000239         2190 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.245, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000239 DAYYCY010000000
VERSION     DAYYCY010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.245"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            328..699
                     /locus_tag="ACHAZB_002691"
     CDS             328..699
                     /locus_tag="ACHAZB_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFM8615303.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(849..921)
                     /locus_tag="ACHAZB_002692"
     tRNA            complement(849..921)
                     /locus_tag="ACHAZB_002692"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(886..888),aa:Gln,seq:ctg)
     gene            complement(967..1395)
                     /locus_tag="ACHAZB_002693"
     CDS             complement(967..1395)
                     /locus_tag="ACHAZB_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8615304.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(1455..>2190)
                     /locus_tag="ACHAZB_002694"
     CDS             complement(1455..>2190)
                     /locus_tag="ACHAZB_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFM8615305.1"
                     /translation="LLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSSTQKTKKTKE
                     KAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDYVPVFNADG
                     THGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLDDIKKALSK
                     GNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIYVNDPYGVK
                     NRAVPINDFSAIFTAMGGQMLTLEKS"
BASE COUNT          691 a          382 c          413 g          704 t
ORIGIN      
        1 tctttataag gtcctaattg ttctttatgt tcttcgataa atcgttttgc gccatcgaaa
       61 ttgccgttct taatcatttc cttgactttt tgaaataatt cttcttgatt cattttgaac
      121 acatcctttg ctatcaacat gattttttac atggacaaaa cgagaaaaac tgatttatcc
      181 acacgtcaat aatatcaaat aacgatcatt tttactaaaa aaacactcga ttctcgcttc
      241 taaagtagga gaaaaatctc caacttggga cggatggaat ttctttaact gacccatata
      301 ctgaatatat aacaaggagg tagaaagatg aaagtgatta aatatttgat tggactggcg
      361 gcgattagtg gtattttgct atttggactg aaatattata ctgaaggaag ttatggagaa
      421 atccctggta tacttgatca gctaaatcca ttagttgaaa aaggagaggt ctatgtaaag
      481 acacaaaaac cagaggaagt aaatgaatat ggtacagcaa ggtatgtgca gaaagctgct
      541 gatgccaatg ggaaagaacg aacggttgaa tttaatggtc tttctgtgtt aaaagaaaat
      601 cattatttga aaattagcaa taaaggagca cacatagaaa cgtatgagga agttcctaga
      661 gaaaaagttc cggagaaagc attagcaatt attgggtaaa tcagtttttg tattaagaat
      721 acgcccgatt ttattattca taatttaatt atgtaaacta aatacaagta gtaaaaataa
      781 tacaataatc cccctgcaaa aagattgttc ttttacaggg ggttcatttt ttcagcaagc
      841 acatcaaatt gggatggcag ggttcgaacc tacaattgct tgattcagag tcaagtgcct
      901 taccagtttg gctacatccc atttattcaa caaaataatc atagcacttt cctaaaaaaa
      961 cgcaagttat ttttcttgat cggatagttg ttgctttact cgtaaaaggc ggcgtactag
     1021 tttcttttct aatccagcag gaagggcaat cagttctgct actgacaaga cggtgtccaa
     1081 taactgatat tctttgacag gttcattcat aatatgatag atgtttgcta aacgttcaat
     1141 atattcaaag cgagtatact ctgttcgagc aattagaaat ttcaataaat cggtggcttt
     1201 ctcgtactct tgatctgcgt aataaacaag agattgacga tacaatttga tttccagttc
     1261 attgagggta gcaggtctgc gacgctcgtt ccactcatta atagagactt taccttgaac
     1321 atttcccttg cagtcacgga caaaaataat cggaatttca tttggatcca tttttttatg
     1381 tgtgaatttt tccattatct acgctccctt caaaggtttg gtatcttgag tataagggat
     1441 ttttgtttga attactagct tttctccaaa gttagcatct gtccacccat tgcagtgaaa
     1501 atcgccgaaa aatcgttgat cggtactgct cgatttttta caccataggg atcgttgaca
     1561 taaatatttt ctttgtcata tccagtgact acacaagcat gaacaagagg agtgactctt
     1621 acttctccat tatttgtggt ccaagtcata aaatcagttt catctgggac ttcaaaattg
     1681 atagtcacag aagtccagac aggatttcct ttagatagtg cttttttgat atcatcgagt
     1741 tgagcatcgg tttttggaaa agtgtgataa tccgtttgga cgatttgctg agcaagggag
     1801 gcaactgggg gaacatagac gaccatagcc cagtctccgc cactgatatc accaacaaat
     1861 ccttcattag gatcgccatg agtcccatct gcattgaaaa cagggacgta atccaattgc
     1921 tcagctaact ggtttttagt tgtttcataa ccataaaaag ataaaagcat agaaagcgaa
     1981 gtgacttcac agccattttc taaagaagga gaatcaaact ggctctctaa tggaacatct
     2041 agtaaaactt ctgctttctc ttttgtcttt ttcgtttttt gtgttgaact tttttgtgca
     2101 gtgttcattg tatgttctgg agaatggata tgtaggtaat aacttgtacc gcttaataaa
     2161 agagcagctg tgccccctag aaacaatagt
//
LOCUS       DAYYCY010000240         2114 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.247, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000240 DAYYCY010000000
VERSION     DAYYCY010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2114
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.247"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1581..>2114
                     /locus_tag="ACHAZB_002695"
     CDS             1581..>2114
                     /locus_tag="ACHAZB_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HFM8615306.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERPVS
                     CGMRTLYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPDNYPHFNSEF
                     GHLEGDTIVGIHHKSAVI"
BASE COUNT          788 a          280 c          422 g          624 t
ORIGIN      
        1 attatcgaac acctttggaa gtatttttga gttacgtaag tattgatgat ctgtctaatt
       61 taatttgaca attaagaaag tattaacttg ttttgtgaaa aagagaattt gaaaaacgat
      121 tggcgaacaa atgctcggta ggtatggtat aaatacagag ctaattggtg tgatgaaatc
      181 agatattcgc gtgagattca gtaaagtagg agttcgaaaa cagctaggtg acaaacagta
      241 ttgctttggt acttcggaat ggtttatcaa attcttatca acagttcact tctctttatg
      301 tttgagtgat gattcgttag gttattgctt ggaaagaccg gatgagagta gaagagttta
      361 attttataaa gtgaaaagag gtaatgtacg atatacgtag ggctgaacca atagaggata
      421 agcaaacgat gttacaggcg caaaattcat agcttactgt tttggagata acagaagagg
      481 aaataatgca gaagtaaggc gaaaaaagga cggaagccta acagcatatg aaataaagaa
      541 aaatattata actactgata aatcggtatt gagcaaagta ctaataggag tcaaaaacat
      601 tcataagcta tgagtgtttc ttggctactt ttttattttt atgtatctac aaaagtttcc
      661 aggggcagaa cctgtgatta ttaatggaag aacctcaagt attgtcttaa atcattgtta
      721 tgaaaatgga ttaaaagagt tcatcaaggt gtgattaaca agttactttt cattacatcg
      781 aatttttcag aagttgctta tattggtgat gatattaatg atttatcatg tatgacatct
      841 gtgaagaagc gggaggaata gtagaatgtc caaaaaatgc cacaagaaat gagattgaat
      901 tatctgattt tattgtaact cacagtggtg gtgacgaatc tgtgatagag tttattgagt
      961 ggatattgga atcgatatag tgtagtagta cagaaaaatt atcatacaat taatactagg
     1021 cataagaata ttggcatatt ctagagttaa aagtaactat tcgaaaagga taaggttaga
     1081 attttttgtt ttgccagtgg aagaatataa aaagggcatt ggaaattaat atattagaat
     1141 gctaagcgga gaaaaaaata tatgaaaagt acgtccttgt gatgggatgg aaaaatacac
     1201 ttatttcaaa caacgataat ataattatgt gaaccgaagt atactcgctt ctaattcaat
     1261 cctcaaatcc cgacagtcgc gacttcaaaa aagcgtatag ttttaatgta tccccatatg
     1321 atccgaaaaa tggtaattta caattaacag tttgatgatg ttgaagatgt ggaaatgctg
     1381 aaattaccat gatgagaaac acattaatta aagggaatta attaactaga atataatctg
     1441 attcaatttg aattaagcaa aaaaaggctg agttttaaat aatcgcgaat tgtaaaatta
     1501 agttagaaaa ataaaaaggc atttatggta cactcaaatt gtatttccga cgaaagaaaa
     1561 caaaggagtg taacccataa atgacctaca aacatcttac catagacgaa ctgacaatga
     1621 tagaatcata ttatcttcaa cataataagc cggttgaaat cgctaaccga atgggacgtg
     1681 ctatacaaac tatttataat gtagtcaata agttcaagca aggcaagact gctcttgatt
     1741 attggcacca gtataaagaa aataagaaaa aatgtggtag aaaagtcatt caattacctg
     1801 ctcatgaagt agattacatt aaagagaaag tcactcttgg ttggacgcct gacgtcatta
     1861 tcgggcgaaa agaaaggcct gtttcatgcg gtatgagaac actttatcgt ttattttcta
     1921 aaggaatatt tgatattgac acactaccga tgaaaggtaa aagaaaaccc aatggccatc
     1981 aggaaaaacg gggaaaacaa cagtatcagc gctcaatcca tgatcgacct gataattatc
     2041 ctcatttcaa ttctgagttt ggtcaccttg aaggtgatac gatcgttggc attcatcata
     2101 aaagtgccgt catt
//
LOCUS       DAYYCY010000241         2104 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.248, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000241 DAYYCY010000000
VERSION     DAYYCY010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.248"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            136..1539
                     /locus_tag="ACHAZB_002696"
     CDS             136..1539
                     /locus_tag="ACHAZB_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4357 domain-containing protein"
                     /protein_id="HFM8615307.1"
                     /translation="MTASDLIRNFIMMDKSNEEQTTLYTKYWRRLEEVFDNSKGMEDF
                     FRYYLAAITGEYSAKHILYQAFKDYWHKERDVSSDAELLQKLVRYAGYFARLYYNKPE
                     GKYSEVLSDFQSMESMMPAPFVLGLSEWYYHDQFITEDQYIDAIKVVNDYQLRRYFNG
                     DDTSRVSKAFPSYLKSVRKYAKANGYENIVDIVIYVLVTRNQSNNMALPSDKALKSNL
                     LNANAYAMRLARWLLEKIENRENSATLDMSNLSIEHIMPQTSTSYWEEKAGTSGEEYT
                     GLVNTIGNLTLVTNPDNSAAGNKDFETKKKIFEDTLHIRMNKDLYELTEWTSSDISAR
                     SEALINELITMYPYLRSSGDYEHDGNREIFLEAQGIKATGYLNEDETVIIHSGSEIYS
                     KIKDIASDSLDETRQELLDNGIIEETIGGLQFVQDYTASSVSNAAALLLGGSRNGWDY
                     WKYDNGISINDSLRNKK"
     gene            1560..1844
                     /locus_tag="ACHAZB_002697"
     CDS             1560..1844
                     /locus_tag="ACHAZB_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcQ family protein"
                     /protein_id="HFM8615308.1"
                     /translation="MDKNKLRYAILKLRDEKKNPFIELKGQVPEEDILEQTKFLSRNG
                     LLESMVWADNTVHIWGSVSLEGEKYLVENSGLAKAYALAKEVKNWIPFWG"
BASE COUNT          731 a          308 c          444 g          621 t
ORIGIN      
        1 ttgatttacc aacatgttta gacattttcc tctaggaaaa aattaactag caccacgcgt
       61 attgtttata ttaaattaga tgaccgtgat aatgcacagc aaatttttga aagtatcaat
      121 tctaccggtg aaagattgac tgcctcggat ttgattcgaa acttcatcat gatggataaa
      181 tctaatgaag agcagactac gctttatact aaatattggc gtaggttgga agaagtcttt
      241 gataattcta aaggaatgga agactttttc agatactacc ttgctgcaat aactggggag
      301 tattcagcaa agcatattct ttatcaagca ttcaaagact attggcataa agagcgcgat
      361 gtatcaagtg atgccgaatt gctccaaaaa ttagtgcgat atgctggtta ttttgcgaga
      421 ctttactata acaaacctga aggaaaatac tcagaagtat tgagcgattt ccaaagtatg
      481 gaaagtatga tgcctgcacc ttttgttttg ggattatctg aatggtacta tcatgaccaa
      541 tttatcactg aagaccaata tattgatgcg attaaggtag tgaatgatta tcagcttagg
      601 agatatttta atggggatga tacaagcaga gtgtcaaaag catttccttc atatttgaaa
      661 agtgttagga agtacgctaa agctaatgga tatgaaaata tcgttgatat tgtaatttat
      721 gttcttgtta ctagaaatca atcaaataat atggcacttc catctgacaa ggcattgaaa
      781 tctaatctat tgaatgccaa tgcttatgcg atgagattgg cccgttggtt acttgaaaaa
      841 atagagaatc gagaaaattc agcaacactt gatatgagta acttgtccat tgaacacatc
      901 atgccacaga ctagtacttc atattgggaa gagaaggctg gtacatcggg ggaagaatac
      961 acaggattag taaataccat tggtaattta acactcgtta caaatcctga taattcggcc
     1021 gcgggaaata aagactttga gaccaaaaag aaaatctttg aagataccct tcatattcgt
     1081 atgaataaag atttgtacga attgactgag tggacatcat cggatatttc tgccagaagt
     1141 gaagctctaa tcaatgaact gattactatg tatccctatt tgagatcgtc gggcgactat
     1201 gaacacgatg ggaataggga aattttctta gaggcgcaag gaataaaagc aactgggtat
     1261 ttgaacgaag acgagaccgt gattatacat tcaggttcag aaatttattc taagattaaa
     1321 gatattgcat ctgattcgct agatgaaact cgccaagagc tgttggataa tggaatcatt
     1381 gaagaaacaa ttgggggact acaatttgtt caagattaca ctgcctcatc tgtttcgaat
     1441 gcagcagctc tgctattagg gggtagccgg aatggttggg actactggaa atatgataac
     1501 ggaataagta ttaatgattc gctaagaaat aagaaataga gcatagaggt ggtgttaggt
     1561 tggataagaa caaactaaga tatgcaattc ttaaattgcg tgatgaaaaa aagaatcctt
     1621 ttattgagct taaagggcaa gttccagaag aagatatttt agaacaaact aaatttcttt
     1681 cgcgcaatgg cttactagaa tccatggttt gggcggataa cactgttcat atctggggga
     1741 gtgtttctct ggaaggggaa aaatatttag ttgaaaactc aggtcttgct aaggcgtatg
     1801 cattggcaaa agaagttaaa aattggattc cattttgggg ctaagataag tttttaaacg
     1861 gagaaggtag caaatcataa ttggtttgtt gccttttttg attgcccaaa aagaaaaatg
     1921 acaaagtata gagataaatg acatagtaca cgggaaaaat gacattgtat caccgacaac
     1981 gtctaaatag atgttgatat atatacgttc cgtacaaata attataaaaa tttaaatgat
     2041 ttgagtagaa gtatcagtga atatctcaag gggaatagct atctcaagat tcgattttca
     2101 cata
//
LOCUS       DAYYCY010000242         2101 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.249, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000242 DAYYCY010000000
VERSION     DAYYCY010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.249"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            59..832
                     /gene="sufC"
                     /locus_tag="ACHAZB_002698"
     CDS             59..832
                     /gene="sufC"
                     /locus_tag="ACHAZB_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFM8615309.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            845..>2101
                     /gene="sufD"
                     /locus_tag="ACHAZB_002699"
     CDS             845..>2101
                     /gene="sufD"
                     /locus_tag="ACHAZB_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFM8615310.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELV"
BASE COUNT          700 a          382 c          486 g          533 t
ORIGIN      
        1 ttcattctca attagtctaa aatgagtgag aacgaaagag aatttggggg tataatgcat
       61 gtctgtctta gaaataaaaa atctacatgt gtcgatcgag gaaaaagaaa tccttaaagg
      121 cgtaaaccta acaatgaaaa ccggagaaat ccatgccatc atgggaccta acggcacagg
      181 aaaatctacc ttatctgccg ctatcatggg caatccaaac tatgaagtaa cagaaggcga
      241 aattttgttt gacgggaaaa atgttttaga cttggaagta gatgaacgtg cgcgacttgg
      301 cttattttta gctatgcaat atccaagtga gataccaggt atcacgaatg cggaattcat
      361 gcgtgcagcg atcaatgcga aacgtgatga aaacgacaaa atttctgtta tggcattttt
      421 gaaaaagtta gatgaaaaaa tggctttact aaatatgcca gaagaaatgg ctgaacgtta
      481 tctgaacgaa ggtttttctg gcggagaaaa gaaacggaat gagatcctgc aattgttgat
      541 gttagaacct acatttgcaa ttttagacga aatcgattca ggtctggata tcgatgcttt
      601 gaaagtcgtt gctaaaggag taaatgaaat gcgcggagac aatttcggtg cattgatcat
      661 cacccactat caacgattat tgaactatat cacacctgat gtcgttcaca tcatgatgga
      721 aggccgagta gtcatgacag ggaatgctga cctagctaaa cgtctggaag cagaaggcta
      781 cgctggtatc agtaaagaat taggaatcga ctataaagaa gaagaagcgt aagggggagc
      841 aagaatgaaa gaaaaaaact ggctcgatta tcttgacgcg gtaaatgatt tttctttaag
      901 taaaggcgaa cctgattgga tgagaacctt ccgtcaagac gcactagcga aagcagatga
      961 actgccattg ccgcatatcg atcgggtgaa attccatcgc tggtcgttat ttgatgtgaa
     1021 agaaacacaa accatcagtg aaacaggcac gattccggca tttgatgcaa tgaaagacaa
     1081 tcctgtgctt gtccaacaag gttcatggac gatttttgaa caactgcctg tggaattagc
     1141 agaaaaagga gtcattttta ccgacttgtt cactgcaatg atcgaatatc cagaactagt
     1201 ccaagaatat tatatgaaaa aagcagtaaa catgaacgaa gaccaattga ctgcactcca
     1261 tgtagctttt atgaacagcg gtattttcct gtatgtgcct aaaaatgtcg tgatcgatga
     1321 accactcgag tccttattca ttcaagatgg cgcatccgat gagcacttct tcaagcatgt
     1381 gttgatcgtg gcagatgaac atagtgaatt ttcttatctg gaacgtttcc agacaaccaa
     1441 agaacaagta gcaaaatcat caggtaatat catcgtggaa gtcatcgcta aagctggttc
     1501 aaaaatcaaa tattcagccg tagatcaatt aggagaaaat attacttctt atatgaatcg
     1561 ccgtgggcac attttacgag atgcttctgt cgactgggca attggtgtca tgaatgacgg
     1621 tcatgtgatc gctgactttg actcagattt agcaggagaa ggtgcacacg cagaagtcaa
     1681 aatcgtggca atcagctcgg gcagacaaat ccaaggaatc gatacacgag taaccaataa
     1741 agcccctcat acgatcgggc atattttgca acacggagtg atccgagaaa aaggaacatt
     1801 gacctttaat ggtatcggcc acatcttgaa aggggcaaaa ggtgcagatg cccaacaaga
     1861 aagtcgtgtc ttgatgcttt ctgataaagc acgtggtgat gcgaatccta ttcttttgat
     1921 cgatgaaaac gaagtaactg ctggacatgc tgccagtgta ggacgagttg atccagaaga
     1981 aatgtactat ctgatgagta gaggattgca taaagaagaa gcagagcgct tagtgattcg
     2041 aggattcctc ggttctgtct taacagcaat ccctgtagaa caagttcgga aagaattagt
     2101 c
//
LOCUS       DAYYCY010000243         2080 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.250, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000243 DAYYCY010000000
VERSION     DAYYCY010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.250"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1033
                     /gene="sppA"
                     /locus_tag="ACHAZB_002700"
     CDS             <1..1033
                     /gene="sppA"
                     /locus_tag="ACHAZB_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFM8615311.1"
                     /translation="NKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKIF
                     YGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQED
                     KAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSDK
                     IFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKVI
                     QEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALDS
                     LKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDTP
                     KPMYYYGGL"
     gene            1035..1556
                     /locus_tag="ACHAZB_002701"
     CDS             1035..1556
                     /locus_tag="ACHAZB_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFM8615312.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            1553..1939
                     /locus_tag="ACHAZB_002702"
     CDS             1553..1939
                     /locus_tag="ACHAZB_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFM8615313.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKTVVYGISLLILSIIPF
                     FLLTLLWVIPGVLGIIAGLMLLIRKS"
BASE COUNT          724 a          260 c          415 g          681 t
ORIGIN      
        1 gaataaaaga agatggattg ctgtgggagt agcgtgcgga ttattgttac tatccattat
       61 cgtcgcgcta attccaggaa aagataaaga ggaagcatca aatacaaccc taacaggaat
      121 aaataagatt ttttacggat ctaacgaaat cacagaagaa acgttagaag aaggcgcatc
      181 aaataaaaag attgtcaaac tatccgtcaa tggagttatt gcagatacag gtgaaagcaa
      241 tctttttagt agggaacaat ataaccatca gaatttttta acacagctaa agaaaattca
      301 agaagacaaa gcggttaagg gagtgttatt agaagtaaat tcacctgggg gaggaatata
      361 tgaaagtgct gagattgcta aagaaatggc aaaaattaaa aagctagata ttcctatcta
      421 tactgcattt aaaaatacag cggcaagtgg agggtactac atttctgctg gttcagataa
      481 aatatttgct acggaagaaa caacaactgg atcaattggt gtcattattt ctggtttaaa
      541 ttattctggc ttattagaaa aacttggcgt aactgatgcg acgtataaaa gtggtgcatt
      601 aaaagatatg atgtcacctc aacacaaacc ttcagaggaa gagaataaag ttatacagga
      661 atttgtgatg agtgcttatg atcgttttgt caatgttgta gcaaaaggaa gaaatatgga
      721 tactaatgca gtgaaagaac ttgcggatgg aagaatttat gatggtaacc aagcagtaga
      781 aaatgggttg gtggatcaaa ttggttactc tgaagatgca ttagattccc taaaaaaaga
      841 gaagaaatta actgatgcga cggttataga atataaaaat gacacaactg gctttgcttc
      901 ttcttggtta ggtaataaga ttgctgaatg gcaagggcta aaggcaacta catcagatcg
      961 tgtaatttca gtttttgaaa aacttggatt ttctgatact ccaaaaccaa tgtattacta
     1021 tggagggcta taaaatgaat aagaagagtg gcattgaaac tttgcctaat tatgtatttg
     1081 cgggattttg gttacgattt tttgcttttc ttattgattt aatttgtatt agcatcatca
     1141 cccatgctac tataggtctc ttttacaaaa tcatgggact ggaatctgac aaatccatta
     1201 ttacttttta tggtatagcg tcattagcta tttatttaac ctattttgta cttttgacta
     1261 agctgaattc aggacaaaca attgggaaaa tgatttttgg gataagagta gtgagctttg
     1321 aggaaaaaga gctaagttgg caaactatct ttattcgtga gtgtatatgt aggtttatat
     1381 taaaaaattt tgtatttcaa attgggtatt tggtggctgc tttcacgccc aagaaacaac
     1441 atattgggga ttatttttct aacacgtcag tggttgttat tcgttcatta gaagctatta
     1501 actatggtca aaaaatttct aattctaaaa cacttaatct ggagagaatt aaatgaataa
     1561 gagaaaaaca gagtttattt tgattttaat tagtggaata acagggattt tatgtagtct
     1621 tttagcattg atgggtggta tgtttctatc ttctattgat tcaactactt taaatactac
     1681 agaactaacc agtaaggata tagatatgtt agtcaatcta agcgggaatg ttgttttatt
     1741 tggaggaatt accctagttt tttcgataat gattttcata ataggttttt tagtaagaaa
     1801 aaatcaaaaa acagtggtct atggtattag tttactcatt ttatctatta ttccattttt
     1861 tcttttaaca ttattgtggg taattcctgg agttttaggt attattgcgg gattgatgct
     1921 tctaataaga aaatcttaat taggcaatgt gtagacaacg tgaaacgatt aatcaattaa
     1981 aagctaaata cgttttgaga aatgataact tgtagtcgat ggaagacatg tagataaaaa
     2041 aactacgtgt cttttattta ttctaatttt ttcgaaaaaa
//
LOCUS       DAYYCY010000244         2079 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.251, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000244 DAYYCY010000000
VERSION     DAYYCY010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.251"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            75..719
                     /gene="pcp"
                     /locus_tag="ACHAZB_002703"
     CDS             75..719
                     /gene="pcp"
                     /locus_tag="ACHAZB_002703"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HFM8615314.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            798..2075
                     /locus_tag="ACHAZB_002704"
     CDS             798..2075
                     /locus_tag="ACHAZB_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8615315.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
BASE COUNT          743 a          387 c          428 g          521 t
ORIGIN      
        1 atttacgctg ttcaaggaaa atggtatgat aaaagataac catatgttaa atcaatcgga
       61 cggaggaaca acagatgaaa gtactagtaa caggttttga tccatttggt ggggacaaag
      121 tgaatccagc ctatgaagca gtaaaaaaaa tgccagatga aatcagcggt gctgaaatca
      181 tcaaagtgga agtaccgacc gtattcgaaa aaagcagtca agtagtcaaa gaagccatcc
      241 aacagcacca accagatgtc gtgatctgtg tcggacaagc aggtgggcgg tcagcagtct
      301 cgttcgaacg tgtcgcaatc aatctagcag aagcaagaat ccctgataat gaaggaaatc
      361 agccattcga tacggcttta gaagaaaacg gtccagccgc ttactttacc tcattgccaa
      421 tcaaagcaat gacaaaaaat gtacaagatc atggtctacc agcgtacatc tcttatacag
      481 caggaacatt tgtctgcaat gatatcatgt atcgtttgct tcatttgatc gaaacacaat
      541 tcccggcaat tcgaggtggg ttcatccacg tgccattctc acccgatcaa gtgatcgatc
      601 gtccagtggg tacgccttct atgtctttag aagacattgc ttcttctttg acttatgcag
      661 ttgaagcagc agtagaaaat aaagaagata tttccggaaa tgccggaaca actcactaat
      721 ttagcaagaa acactggctg gttgctaacg ttatgttagt agccagcttt ttgtaattag
      781 aaaggaaaga aacagccgtg gaagaatggc aatccgtctt tgaagaatgg tttccaaaag
      841 aaatcagcaa aagctatccc atcaaaattt ctaaacaata cacaagcagt caacgttggg
      901 agatctatgc aaaattaacg aaaaaacagc gggaactagt cgataagcac cgccgatatc
      961 taatcagctc tcgttttatg gaagaacatt atttagctgc aacagattgg gtgttttctg
     1021 acttcaagat caatccgttt tttcgaacaa aacgaagcca gcaaaaattg tattgtgagt
     1081 gtggtagaga attaaaagtg caatacatcg ttaaatcgcc aaaaaccgga aagatcctaa
     1141 aactaggcat caaccacttt gccgatcacc tgcatgtctc tccgacagtg gcagcttcga
     1201 tccatcaggg aatgacaaaa gttgatctgg ccttagacga attattatgg cttaagcaga
     1261 agaatatcga ttttcctgaa ggactatggc aaaaatactg ttttgtcttg taccaaaatc
     1321 gtcggatgaa acagccatat ttaccggata taaaattagc tcagcggctc gcagaatttc
     1381 gccaagtaga aatgccgatt tacatagcgg attatcaagc attggaaaat gaaatcaaga
     1441 aaataagtga acacatcaat gggcagtcaa aaaagcgaca gataaaaaaa gaactatttg
     1501 acgactttgc agaagaatta gtaaaggatg tagaagaatt cttgatcaac tatcgcgcat
     1561 ttttacgtaa agattggcaa tccatcgtat atgaagaagt gccagtacat cctaatgctt
     1621 attttgaaac atttatttcc gtgttgagaa agacaaaacg tcaacgaaca ccagaagtca
     1681 ctgctcagat ggaatatttt gcgaaaaacc aacgattcat ccaaccaaaa atctatctat
     1741 ttatttggaa gcaatattgt cgctatggtt ttacagaagg gttcttcgac agcattcctc
     1801 ggatagtcag aaatggtttt ttaaaagtat tgcgtaaaga acgagaagct atacaatctg
     1861 ctgataagaa agaccgtaca gtctcaaaag aaaaatggca actagtagtg aaagatatcc
     1921 aatctggaaa tgtgcaagag acaatcgaca aatggaaagg aaaacattat cgttttactg
     1981 aagctcagaa acaagcgttg gaatattatc agaagttaga agaatcactg cgtttcaatg
     2041 atgaggcaag aaagtatctg aaggaattac tataaaaaa
//
LOCUS       DAYYCY010000245         2065 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.252, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000245 DAYYCY010000000
VERSION     DAYYCY010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.252"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1008
                     /locus_tag="ACHAZB_002705"
     CDS             <1..1008
                     /locus_tag="ACHAZB_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8615316.1"
                     /translation="LLDAPYYLSKVALGENLIKPESQQPVTNSNYLINTYNIKPMVKG
                     KKYTITLEGTKPATQVFRPFFTRATGDAWEVGDLQPVEGLTDVWSGTFTAAADPHPTS
                     PQVQICQVPSTSLGNCTIKWLKLEEGDTRTPNIEQYKYRGIGMRDSNNPKDYVWDLAP
                     EYVEDNLATDIKISEITGKANNYTDGKVSEINSQLTASINEVDTTAKDAQTKANANAT
                     AIDELDNKIDERINDTATTTLTVTNGNTGSAKLYREGKTVSIYFVALNGRSSGGNDST
                     ILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSDYTFAITYT
                     I"
     gene            1025..1174
                     /locus_tag="ACHAZB_002706"
     CDS             1025..1174
                     /locus_tag="ACHAZB_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8615317.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            1171..1617
                     /locus_tag="ACHAZB_002707"
     CDS             1171..1617
                     /locus_tag="ACHAZB_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8615318.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            1619..1756
                     /locus_tag="ACHAZB_002708"
     CDS             1619..1756
                     /locus_tag="ACHAZB_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFM8615319.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            1794..>2065
                     /locus_tag="ACHAZB_002709"
     CDS             1794..>2065
                     /locus_tag="ACHAZB_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8615320.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKT"
BASE COUNT          753 a          358 c          425 g          529 t
ORIGIN      
        1 ctacttgatg caccgtatta tttgagtaag gtggctttgg gtgagaatct aattaaacca
       61 gaatcacaac aaccagttac taatagtaac tatcttatta acacctataa cattaaacca
      121 atggtaaaag gtaagaagta taccatcaca cttgaaggaa ctaagccagc aacacaggtt
      181 tttagaccat ttttcacacg agcaacagga gatgcatggg aggttggtga cttacaacca
      241 gtagaagggt taactgatgt ttggtctgga acatttacag cagcagctga cccacaccct
      301 accagcccac aagttcagat ttgtcaggta ccaagcacaa gtttaggaaa ctgtacaatt
      361 aaatggttaa aactagagga aggcgacaca cgaaccccga atattgagca atataaatac
      421 cgaggaatcg gcatgcgaga ctcaaacaat ccaaaagatt acgtctggga tctagcacca
      481 gaatatgtcg aagacaatct ggccacagat attaaaattt ctgaaattac tggtaaagca
      541 aacaattata ccgatgggaa agtatcggag attaattcgc agttgactgc ttcaattaat
      601 gaagtagaca ccacagctaa ggatgctcaa acaaaagcga atgctaatgc gactgctata
      661 gatgaattag acaataagat cgatgaacgc attaatgata cagctactac cacattaaca
      721 gttacaaacg ggaataccgg atcagcaaag ctttatcgtg aaggaaaaac agtttctata
      781 tattttgtgg ctttaaacgg aagaagcagt ggtggaaatg attcaacgat actaacaatt
      841 ccagaaggct atcggccacc aattagtttt gagcaactgg ttggctcgat agaccgttct
      901 actttgaaca gtgctcagtt atctattggt gcagatggag ccattaaatg gcgaagaaac
      961 tcaagttatg gatcggatta tacctttgca attacttaca cgatttagaa aagcgtgaat
     1021 cgatatgaag gcagcatata gaccaattga accttacgga ttcgagcaaa tcattgtgaa
     1081 tgatgaagaa catttaccgg aagaatgcac agaagtcgaa ccaccgattc caaattggaa
     1141 accgaaattc aattactggg agggaaataa atgaaaaaca tttggaaata tggacgtact
     1201 ggcggagagt acgcaggaaa agtattggac gacatgcttg tatccgttcc ttacacagat
     1261 cagccaccgc ttgaaggaat tcgtgctgat ggcgaaccgt taacaatcgc tgatcaaatg
     1321 tttgatccta aattgaatca atggattatt ttagcgaacg cactagatca caacgattta
     1381 aacaatctca aagcaatgta tgagtcgtta gaaaatgaga acggcgattt aaaacagatc
     1441 aatgccaaac tcatgctaag cgatgtagca attaaacagg aaaatactgc attgaaagaa
     1501 aaagcggata gtttagcaca aatcaattca aaaatgatgc ttgcttcgtt acaaaatagc
     1561 aaagacattt cagaaattaa agagcaacta aatccagctt caaagggagg tgagtagtat
     1621 gtttagtttt agcgatgtga aaatgatgta tgattggggc tgtttcacta acgaacaagt
     1681 aatggttttc gttccgttgt gcattactga agaaaaagca gataaaatca ttagcaaaga
     1741 agagagcgca tcttaattga tgtgcttttt attttgattc aaggagttgt cacatgatta
     1801 atttagggga atggggagcg atagcaggat caataaccgc tatcgtttct ttgattttat
     1861 tagtaataaa accaattact gcatctttct cgaagattac tgagactctt tcaaaagtaa
     1921 gtcacaattt agatttgctg actaaagatt tagaatcgag caaatcagat cgattgatga
     1981 ttcatgaaga actaaagaaa cacgatgaaa gattagatac acatgcagaa aaattggtag
     2041 aacacacgca acaaattaaa acttt
//
LOCUS       DAYYCY010000246         2041 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.253, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000246 DAYYCY010000000
VERSION     DAYYCY010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.253"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            34..1056
                     /locus_tag="ACHAZB_002710"
     CDS             34..1056
                     /locus_tag="ACHAZB_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide synthesis protein"
                     /protein_id="HFM8615321.1"
                     /translation="MTQKHSSIKRIIHRISEELNATRRIAKITSWKEAFATLQAKIDI
                     QVMNRNGYNESKKQKEHLLKKHEVMLKYYEKTFSDFLKTYDYSQNQIINQSKSPYSNC
                     IWVCWWQGLDQSPEIVKACVKSIKRNAGKHTVIILTEDNYRQYVDIPKWVEEKKNKGI
                     ITRTNYSDLLRLSLLAKHGGLWLDATFYCTKPIPDEYFDIDIWSIRRPDYAHASVASG
                     YFAGYSLACNQESRWMFAIIRDFFLNYWKNNDMMVDYLMIDYMIVLAQRINHEIAQTF
                     DKIPSNNPLCDELIKNLNNIYDEKVWNLLKKDTLLFKLTWKQSFKKQKDDKVTFYGML
                     VENKLL"
     gene            1078..>2041
                     /locus_tag="ACHAZB_002711"
     CDS             1078..>2041
                     /locus_tag="ACHAZB_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM8615322.1"
                     /translation="MKHKVSVIVPIYNVEKYLRKCVDSLLSQTLKEIEIVLVDDGSPD
                     ASGEIADEYQKKYSNVKTIHRENGGLGPARNTGIENATGEYVAFLDSDDWVESDMYEK
                     LYLVASKENADIVVSGHCDFANDKAMVKKQHPLSGNVYDTPNSIKNVRKNLFGHSPKD
                     QEVEAFPMSVCMSLYNKNLLDKYRLRFEKILSEDTIFNLNAYDVANKIAFTEYTDYCY
                     RKEEQESITKSLSPNKQTEFKEFLITLKAIASKEDLECQIRAKRMAIDYCRLYVGLVD
                     DANLGIKEKRKYIKKFIEDEGLIQCWKGYPTNTLPFQQKFFHKML"
BASE COUNT          821 a          215 c          388 g          617 t
ORIGIN      
        1 gttatttgaa ttaatacaaa gaggttaaag aatatgacac agaaacattc aagtataaaa
       61 agaattatac atcgcatatc cgaagaattg aatgcaacaa gacgaattgc aaagataaca
      121 tcttggaagg aagcatttgc cacgttacaa gctaaaattg atattcaagt catgaatcgt
      181 aatggatata atgaaagtaa aaaacagaag gaacatttat taaaaaaaca tgaggtaatg
      241 ctgaagtatt atgaaaagac tttttcagat tttttgaaaa catatgatta tagtcaaaat
      301 caaataatta atcaatcaaa atcaccatat tctaactgca tttgggtttg ttggtggcag
      361 ggattggatc aatcgccgga aattgtaaaa gcttgtgtaa aatcaataaa acgaaatgca
      421 gggaaacata ctgttataat tttaacagaa gataattata gacaatatgt tgatatacca
      481 aaatgggtgg aagaaaagaa aaacaaagga atcattacga gaacaaatta ttctgattta
      541 ttaagattat ctttgctagc aaaacatgga ggtttgtggt tagacgccac attttattgt
      601 acaaaaccaa taccagatga gtattttgat atcgatattt ggtctattag gaggcctgat
      661 tatgctcacg catccgtagc atcaggatat tttgcgggtt attcattagc atgtaatcaa
      721 gagtctagat ggatgtttgc tataattaga gacttttttt taaattattg gaaaaataat
      781 gatatgatgg tggattattt gatgatagat tatatgattg ttttagcaca gagaataaat
      841 catgaaatag cacagacatt tgataaaata cctagtaaca atccattatg tgacgaattg
      901 attaaaaatt taaataatat atatgatgaa aaagtatgga acttattaaa gaaagatact
      961 ttactgttta agttgacatg gaaacaatct tttaaaaagc aaaaagatga taaagttact
     1021 ttttatggga tgttggtaga aaacaaatta ttataaatat tagaggtata aaaagttatg
     1081 aaacacaagg taagtgtgat agtaccaatt tacaatgttg aaaaatattt aagaaaatgt
     1141 gtggatagtt tattgagtca gacattaaaa gagatagaaa tcgtattagt tgatgatggt
     1201 tcgccagatg cttcggggga aatagcagat gagtatcaaa aaaaatattc taatgtaaaa
     1261 acaatacata gagaaaatgg ggggttagga ccggcaagaa atacaggaat tgagaatgcg
     1321 acaggagaat atgtggcttt tcttgattca gatgattggg tagaaagtga tatgtatgaa
     1381 aaattatact tagttgcgtc caaagaaaat gctgatatag tagttagtgg acattgtgat
     1441 tttgcgaatg acaaagctat ggtgaaaaag caacatcctt tgagtgggaa tgtatatgat
     1501 acgcctaata gtattaaaaa tgtacgaaaa aatttatttg gacatagtcc aaaagatcaa
     1561 gaagtagagg ccttcccgat gtcagtatgt atgtctttgt ataataaaaa tctgctagat
     1621 aaatatagac taagatttga aaaaatttta tctgaagata caatttttaa cttgaatgca
     1681 tatgatgtcg caaataaaat tgcttttaca gaatatacag attattgtta tagaaaagaa
     1741 gaacaagagt ctattacaaa aagtttatct ccaaataagc agacggaatt taaagaattt
     1801 ttaataactc taaaagctat agcatcgaaa gaagatttag aatgtcaaat cagagcaaaa
     1861 cgtatggcaa ttgattattg taggctttat gtgggcttag ttgatgatgc aaatttggga
     1921 attaaagaga aaagaaagta tataaaaaaa tttattgaag atgagggatt aattcaatgt
     1981 tggaagggat atcctacaaa tacgctacca tttcaacaaa aattttttca taaaatgctt
     2041 g
//
LOCUS       DAYYCY010000247         2036 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.254, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000247 DAYYCY010000000
VERSION     DAYYCY010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.254"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            22..969
                     /locus_tag="ACHAZB_002712"
     CDS             22..969
                     /locus_tag="ACHAZB_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8615323.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            1099..1839
                     /locus_tag="ACHAZB_002713"
     CDS             1099..1839
                     /locus_tag="ACHAZB_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8615324.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT          716 a          339 c          425 g          556 t
ORIGIN      
        1 catgttagaa aggaagagag catgcccact ttagcagatg ttgcaaaacg tgccaatgtt
       61 tcaaaaatga cggtctctcg agtcatcaat catccggaac aggtgactga cgaattgaaa
      121 gagttagttt tctcagcaat ggctgaactg gattaccgtc caaatattgc tgcaaaagca
      181 cttgtcacga atcggtcgca gatcatcaaa ttatttatac tggaagaaat cgacacaact
      241 gagccctatt acatgaattt gctgatggga atcgctaaac ggatcggtaa agcgcattat
      301 tctctccaat tagttacaaa tgatgccttc gacgtcggtt cttgtgatgg atacatcatc
      361 acaggtgtca gaaaaaaaga cttcgattgg atcgaccgtt tggaaaaacc agttgtcttg
      421 tttggggaaa attctgaagg atatgattat gtagacagcg ataatgaata tggtacagca
      481 aaagcaacgc aatatgcctt agaaagagat tatcaaacga ttatttatat cggtatcgaa
      541 gtggacgagc cttttgaaat ttcaagggaa actggttatt tatctgtgat gaaggaagtt
      601 gatcttccta gtaaaatctt gcggtttcca aatcattcga cagaagtaga aagatatatg
      661 acagaaaact gggcagattt tacagataaa acttgttttg tatgtagttc agatcggcta
      721 gcactaggag cagtaagagg gattataaat gctggaggac acttgcccga agaatttggt
      781 gtgattggat ttgatggggt atttttagac caaatcagtt cacctaaatt gacaacgatc
      841 aagcaagata ttatacgtat gggtgaagta tgtggagaaa tgttgttaaa aaaaatagaa
      901 gaaaaagggg cgagtcaagg ttatcgtcgt ttttttcctg aactagttat cagagagaca
      961 acgaattaag tcttattact atatatatat ttatgaaatt catcgctatt ttcttaaaat
     1021 aatcagcgca ttgctactga aaacaatgaa tctgtgctat tattgagaaa agttccattt
     1081 ttgagaagga tggtttgaat gaaagaaatc agacgaaaac atttccgttt tcctgcgtac
     1141 gatgatgagt taggcgtaaa attaaccaac gacaagaagc gagtattatt tgacggacaa
     1201 gaagatcttt tgactgaaca gaatcagtcc ccgtttgaac agatgaaaga atttcatttc
     1261 acgaacacga acaccgagaa aatccgtcgc aagcgtcgtg caacaaatgg attgaagtca
     1321 gctccgcgta aggaaaattt ggaaaagcat aaaggaaaat taccggatta taccggccat
     1381 taccaatcag aaatcacatc gacaggacga aaaaaacttt ttggtgattc ctctcgttct
     1441 gtcagacaaa gtgaaacaag gacagagtcc aagcatacta cacgaaacgc atctgttgac
     1501 acgaaggtgc gggagcgttc ttattttgtt cctaaatatg tacccgcatc gattatccca
     1561 gaccctagag aaagcaaaat ttctgaggat catttaatcg aatcgatgaa aaaaacggat
     1621 gactcttatc tgttgttcga tacagaaccg acgccgtatc aagaaaaaag aaatggtgag
     1681 ccgtctgtac gaacattcaa tcgaacaaaa acgttagagg aaacaaagca gtcagaagaa
     1741 aaaaaatcga aaaaaagcca tggtatctta gaacgctcat tacaaggatt gatcgatgat
     1801 caaggaaact ctttgggaga aaatagctat tttaaataag tgatatacaa atttatattc
     1861 ggctacagtt ggaaaggaaa cgctgcggcg tttccttatt ttatactcta tccgaaagtt
     1921 tatatcgtat cctcaaattt ttttgagttt aagcgaattt tttctcccaa taagtaagaa
     1981 aataatggca aaataaaaaa acaaactatt ttcgcagatc gtttgcaaaa atagtt
//
LOCUS       DAYYCY010000248         2013 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.255, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000248 DAYYCY010000000
VERSION     DAYYCY010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.255"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..914)
                     /locus_tag="ACHAZB_002714"
     CDS             complement(<1..914)
                     /locus_tag="ACHAZB_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HFM8615325.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1342..1905
                     /gene="efp"
                     /locus_tag="ACHAZB_002715"
     CDS             1342..1905
                     /gene="efp"
                     /locus_tag="ACHAZB_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFM8615326.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
BASE COUNT          667 a          368 c          394 g          584 t
ORIGIN      
        1 aagtaaatgt tttttgataa atagcggtct gctgcgtctg gaaaaatcgt aacgacacga
       61 tgaccaggtg gtaaacgacg aatttctctt aatgctgctg caaaagccgc accactcgaa
      121 cttccaacta atagtccttg ttctcgtgct aactgcctag tatagttaaa cccttctaca
      181 tcagagattg tctcgatctg attgattgaa aggggagaaa gaaacggcgg aatgaattct
      241 acaccgatac cctcaatttc gtgaggggct ggatcaccac catttaacac agagccctct
      301 ggttctacac cgattattcg aatgtcggga tatttttctt tcaaaaaggt agaagttcca
      361 gcaaaggttc caccactccc aattcctgct acaaaactat ggatattttc tttgatttca
      421 tgaaagattt ctggccctaa cgtatgatag taagcagcag gattatcttt attttcaaat
      481 tgaagaggca gatagctgtt tgatatctcc tctgcaagtt ttttactttt gctgatggca
      541 ccgatgattc cttgctcact aggagaatgg atgatctttg ctcccaatgc tttcatcaat
      601 tgttgttttt ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa tttgtatgtg
      661 agtgcggcca gagcgatccc aattcctgta tttccggcag ttggttcgat gatcgtcgtt
      721 ttttcattga tttttccttg ggcaaatcct tctttgatca aataactgcc caatctatct
      781 ttgatacttc ctccaggatt taaatattct agttttgcat aaatagcaga acctttaggc
      841 atagaatagt gctgattgtc aaagcgataa agcggtgtgg aaccaatagc ttctgtaatt
      901 gtcgagataa tcatgtgggc ctcctaaaaa aagataataa aaaaaggagc tgactattaa
      961 atcagctcct tgaaagacat agagaaaaat tgagtagata aaaagataag ttacctggta
     1021 ctcattctat gaagggcaat taatagacat gacaaatagt atgctgcttt tatgcaccat
     1081 acaacagcaa cgattcaatt gtttttggtt gctgactgtc atgtgtatcc ctccaagtaa
     1141 aatttttatt aacgatagca tgaaaaaaaa taaaagtcaa aggcttgaaa gaaaaaaata
     1201 tttcgatagc atcgtttacc cgtgtttatt tttctgacaa ataggcatga gaccttaaaa
     1261 gatagagaat actctagttt tctggccggt tgtctgatac aataaagttg attatgataa
     1321 agaaaagaag gagcaaaaat aatgatctca gcaagtgatt taagagccgg tatgactttc
     1381 gtacaagacg gcaaactgat taaagttctg gatgcaagcc accataaacc tggtaaagga
     1441 aatacagtta tgcgtatgaa actgaaagat gtccgttcag gtgcaacata cgacacaacg
     1501 ttcagaccag aagaaaaatt tgaaaaagca catatcgaca caaaacctgt acaatatttg
     1561 tacacgatgg atgatacagc tttcttcatg gatttggaaa catatgagca atatgaaatt
     1621 cctgtagaaa cagtagaaga agaaatgaaa tatatcctag aaaacatgga agtaaaaatc
     1681 caatttttcg gtagcgaagt catcggcgtt caattaccaa caactgtcgt attacgtgtt
     1741 gtggaaacac aaccttccat caaaggtgca acagtcactg gttcaggtaa gccagcgaca
     1801 atggaaactg gcttagtagt caatgttcct gacttcatcg aaacagatga attgttggaa
     1861 atcaatacag cagaaggttc ttatgtaaaa cgtgcgtcaa aataattcgc taacggattg
     1921 ataagaattt gaaaacagag cttgacctct tgaaaggcag atcatcggat ctgcctttca
     1981 agaggttttt tgtggaatca gcaaattgaa aaa
//
LOCUS       DAYYCY010000249         2003 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.256, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000249 DAYYCY010000000
VERSION     DAYYCY010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.256"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..378
                     /locus_tag="ACHAZB_002716"
     CDS             <1..378
                     /locus_tag="ACHAZB_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFM8615327.1"
                     /translation="KNVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFR
                     RFPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTD
                     DWQKVMAAEVIATYLDGDLVYKK"
     gene            426..1826
                     /locus_tag="ACHAZB_002717"
     CDS             426..1826
                     /locus_tag="ACHAZB_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFM8615328.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
BASE COUNT          555 a          339 c          432 g          677 t
ORIGIN      
        1 aaaaatgttt ccacagagga cttctttaat tattgtcgat tagaggaaag tggtgtaaca
       61 gtcactagcg gtacagattt accgctgttt attactgata ttcctgaatc gattataaga
      121 gcagtattcc gtcgttttcc tgaaggagaa gaagtctggg agaaagaaac aagcttcacg
      181 acaaccgagc tgctgaaaag ttttacaagc aacagcttca aagtaaatgg tttatcggag
      241 tacggagaaa taaaagtagg gaaaagcgcc agcttagctg ttttcagcga ggacctctgt
      301 actgatgact ggcaaaaagt gatggctgcg gaagttatcg ctacctatct tgatggtgac
      361 ttagtttaca agaaataatg cttttcgata taacgttata aaacgaatta aagagaggtt
      421 aaaaaatgga aacaatgaaa agaaaattca ccttcccaca tagttataca ttgattttta
      481 tcttaattat tttggctgca attttaactt ggataattcc tagtggtcag tttgagacag
      541 ttgttgaaaa cgtcgcaggt atggaaaaaa caacggttgt tccgggaacc tatcaccaaa
      601 tcgataaggc agattatgga caaggttttt cctctatact aatggcacca gctgaaggga
      661 ttatttcagc tgttgaggtt gttgcttttg ttctcatcgt tggtggtgcc ttcgggatta
      721 tcctaaaaac gggagccatc gatcgagggc tgtttacctt agcaaaagtt ctgggagaca
      781 aggggatctt agttattccg atgtcgatga ttctattcag cttaggagga tcaacctttg
      841 gcatgagcga ggaagtcatt cccttatttg cggtgtttat ttctctgatg ttttctcttg
      901 gctatgattc catgactgcg atcttgattc tatttttaag tacacaagcg ggatatgttg
      961 ggtcgacgat taatcccttc aacgttttaa tcgcacaggg agtagctggc gtttacggaa
     1021 atcccctgct ttggtttcgg atgctatcct ggattgtcat tactgggatt tcaattgttt
     1081 tcaccatgct ttatgctagc agagtcaaaa agaatccact gtcctctatt gtttatgaga
     1141 gtgatttaaa ggttaagcat aagtttcaaa attttgacac tgaaaaagag ttttcaacac
     1201 aagataaact aattatcgcc ggttttatcg taggtttagg aatcatgatt tggggtatca
     1261 tttccaaggg atggtatatg gttgaaattt ctgcactatt cttaggatta ggtctttttg
     1321 ctgggggcat cgctagattt tctcaaaagg aaattgctga agcttttgtc gaaggctgtt
     1381 cagatttcgc ctatgctgct gtaatcattg ggctagctag aggagtcttg gtaatcttgg
     1441 aaggtggaat gattattgac actattctaa acagcttggc taacctttta ggtggtcttc
     1501 caaaattcct gttcactacg attctactca tttctcaagt aattgtttcc ttttttgttc
     1561 catcctcctc aggagctgcg gcattaacga tgccggttat ggcaccattg ggtgatctaa
     1621 tgaatgtaag tcgtgatgtt gttgttctaa gtaaccagtt tggaaatggt ttaatgaata
     1681 ttatctcccc aactagtggt gtattactgg caggattagc catcgcagag attaatttct
     1741 ctaaatggtt taaggttggc ttcaaaatat ttgccatctt aacccttgtt tctgctgttt
     1801 tgttatacat tgctacactc ttttagtctt aatcatccat taataggagg caatagttat
     1861 gattaaaata aatcaattag caggaatgaa tatccactat ttattttatt ctctcaaaga
     1921 atttttcgct tatcaacaga aaattggtat ttcttctgtt gaactttggg gaggtgctcc
     1981 gcattttttc atagatgctt tta
//
LOCUS       DAYYCY010000250         1991 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.257, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000250 DAYYCY010000000
VERSION     DAYYCY010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1991
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.257"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            273..839
                     /gene="amaP"
                     /locus_tag="ACHAZB_002718"
     CDS             273..839
                     /gene="amaP"
                     /locus_tag="ACHAZB_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFM8615329.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            852..1040
                     /locus_tag="ACHAZB_002719"
     CDS             852..1040
                     /locus_tag="ACHAZB_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFM8615330.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1053..1610
                     /locus_tag="ACHAZB_002720"
     CDS             1053..1610
                     /locus_tag="ACHAZB_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFM8615331.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1642..1884
                     /locus_tag="ACHAZB_002721"
     CDS             1642..1884
                     /locus_tag="ACHAZB_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFM8615332.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          724 a          308 c          406 g          553 t
ORIGIN      
        1 aataaataga atcttgtaat aataactgag cataatcttt gcaattttta atcttgaata
       61 atatccttta agtatagcca gcaatcaaaa gtaaatagag aataagtaag caattgttat
      121 ttcagtatca gactggcaat aaaacgccgt tatgatccag caatgtttgg tagcgcttta
      181 tttactgatg ctatgctaac tgttcgctat ctatactgtg attgtcggta gagttattta
      241 cagggtaaaa ataagtttat aggaggtgga aaatgaataa ggggttaaaa ataatcggtg
      301 ttatcatgag tttactgcta ctttcagtat ttctagcgat tgcgttgatc aatgcgccgt
      361 atattatgcc tgaacaattg gaacgatttc gcttctttac tttaacgaat tactacatgc
      421 aacaatatat tttctgggca gctgtagtat ttgccgttct tgctattatc atcttgctag
      481 tcgttctttt ctatccaaag tcaagaggaa ctttcgttat gaaacgggaa gatggcaaac
      541 taacaatcga caaaaaagcg atcgaagggt tggtacgttc ccatttacac gaagaagagt
      601 tcattcattc acctaaagta cggatacgtt caacgaaaaa tcgaatccat atccatgtca
      661 acggagactt gaaacgaact tcttcattag tagggaaaac aggtgtattg atgcaagata
      721 tcgaagaaga agtaactaaa gtactcggaa caaaagaaac ggtcaaagtg gcagtcacat
      781 acagcggtta tgaagaacaa gaagatacaa gagactacaa gcactctcga gtggaatagg
      841 agggccaaat aatgaaagaa ctattttcag catacaagct ccctattatc tttgggggtc
      901 ttggactact gctagctgtt ttatggttaa cgattggatt tttcaagaca ttgttgatca
      961 taatcatgac agcaattggc gcaggaatcg gtttttattt gaaacagtca ggaatagttg
     1021 atcaattttt taaatcatag gaggaaacaa agatggaaaa caaaaacatg aatacaacaa
     1081 atggatcagt tttggcaaat gaaaaaggag aaaaccgcat gatgaatggt gaattgactt
     1141 atgaagacaa agtcatccaa aaaatcatcg gttatgcatt gaaagatgta gatggtctat
     1201 tgacattaga tggcggattt ttctcaaata tggctgaaaa attagtcaac acagataacg
     1261 taacagcagg tatcaataca gaagtaggta agaaacaagt agctgtggat ctagatgtga
     1321 tcgtagaata cggtaaagac atcgaagata tttacaacca aatcaaagaa ttgatcagta
     1381 ctgaagtaaa caatatgaca catttagatg ttatcgaagt gaatgtgaat gttgcagaca
     1441 tcaaaacaca agaagaatac caaaaagatc aagaaacagt acaagacaaa gtaacagaag
     1501 cagcaaaatc cacaggacaa tttgcttcac gccaaacaga taaagcaaaa tctgctgtag
     1561 gcaaaggtgc acaaaaagtc aaagaaaata acgaaccacg tgtacaataa gaagtaagaa
     1621 gtcagttagg aggaatttaa aatgggattt atttgggcat taatcgtcgg cggtatcatt
     1681 ggagcaattg caggagcaat cacgaataaa ggatcatcaa tggggatcat cgcaaacatc
     1741 attgctggtt tagtaggttc agcaattgga caagctttac taggaacttg gggaccaaca
     1801 attgcaggaa tggctatcat tccatcattg atcggtgcaa tcattttagt attagtcgtt
     1861 tcattctttg cacgtaaatt ataagcaaat tgaagaaaaa cttatgttga aatgaagaac
     1921 aaatttgaga accgtagcga cactcgctgc ggttcttttt tatatatttt atatgaagta
     1981 ttctttattc c
//
LOCUS       DAYYCY010000251         1970 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.258, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000251 DAYYCY010000000
VERSION     DAYYCY010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1970
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.258"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            139..753
                     /locus_tag="ACHAZB_002722"
     CDS             139..753
                     /locus_tag="ACHAZB_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8615333.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(918..1742)
                     /locus_tag="ACHAZB_002723"
     CDS             complement(918..1742)
                     /locus_tag="ACHAZB_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8615334.1"
                     /translation="MIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLELK
                     TLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITACE
                     IGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEKRN
                     KLVHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSFMYRDFYEESIELTPTEPNKSQ
                     ITLSKELERKVESQELAIGISITNVSTYKELYEAKSQDNKDFVENIDKEPTNTVGD"
     gene            complement(1766..1921)
                     /locus_tag="ACHAZB_002724"
     CDS             complement(1766..1921)
                     /locus_tag="ACHAZB_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8615335.1"
                     /translation="MEYKFNLNLKEVLPTQGTININGQDNDDDIVIKKIEFIDSDINV
                     FFILKKN"
BASE COUNT          658 a          333 c          287 g          692 t
ORIGIN      
        1 aatgtcataa tcgtttaaat tttcttgatt gacatttatt tacaaaaaat attgccattt
       61 aaaaagaaaa gtgacatatg gcttcctcgt gtatgattag ggtgtatttt aaatagttgc
      121 agaaagtagg taaaagtttt gagagttgca tacatacgtg tctcatcgat cgatcaaaat
      181 gaacaacgcc agattgaaga aatgaaaaaa ttcggtgcag agagaatttt catcgagaaa
      241 caatctggcg caacgattac tcatcgacct gttttccaag aagcattaga ttacgtccga
      301 gatcaagata tttttattgt ggaagctatt gatcgacttg gaagaaatta tgacgagatc
      361 atcgcttcag tcaattactt gaagaagaaa aatgtgaagc tgatcattac tagtttaccg
      421 atcatggctg aagcaattgg caatcctctc ctggataaat tcattaagga tctgatcatc
      481 caaattttgg ccatgatcgc tgaacaagaa cggactgaat cgaaacgtcg ccaggcacaa
      541 ggaattaaga tcgcaaaagc aaacggcgtc tacaaagggc ggcccaaatt atacagtgca
      601 gacgcaaaag atccccaacg tcgccttgtt tacaaaagca tcgttgaaga tcttaaaaat
      661 ggtgttgcga ttgctaagat tgctaaagac tacaatgtta cgagacaaac cgtgtataga
      721 ataaaaaaag acagcatggt caatgacaaa tagttgatca tgctgctttt attatcttct
      781 catctttaat ttcctacata taattactat ctataaaatt aagtcttact cagaacagat
      841 aaagcataaa attccaacaa accatttttt acttaactaa ataaagagtc tgttaatata
      901 atcttcactt atttacgtta atcccctact gtatttgttg gttccttatc tatattttct
      961 acaaaatcct tattatcttg tgatttagct tcgtataatt ctttgtatgt cgacacattg
     1021 gttatactaa ttcctatagc tagttcttga gactctactt ttctctctag ctcctttgac
     1081 aaagtgattt gagatttatt tggttccgtt ggagtcaatt ctatagattc ttcataaaaa
     1141 tctctataca taaagctatc attaatttta taaaaaaaat taaatgtttt tagcactgcc
     1201 atataacatt caaaacattc ttctatagtt ggtttacttc cttgaggaga atgaaccaat
     1261 ttattacgtt tttctatgaa atttttaatt tcctttctta tttctttcgg aaactcaata
     1321 ttaaagtacc ctttaaacat gctccctaat aatttgtcta cagggggaga ttgtaaattc
     1381 tcaaatatta gagttaaatc aggtttctct tgttgataaa attcctttac ccctatttca
     1441 caggctgtaa ttatcgaaat aaacctatct ctagtatttg atatattttt acttctttta
     1501 aggagtataa gagaaaaggg gatactttcg tgtccatgca tgtaattaac taattgttga
     1561 gcttcagaag ttatatctct aattatatag ttaacggaat taggaagtgt ctttaactct
     1621 aatgaaattt ctatccaatt actatcttct aatctgtata tgctcgaagt ataatttgat
     1681 tcgtcagcaa gcatcgctac atcccaaaca ctgatcataa aatcttttaa acgttcaatc
     1741 atttcagcaa tattgctaaa atcagctaat ttttctttag aataaaaaaa acatttatat
     1801 cagaatcaat aaattctatt ttttttatta ctatatcgtc atcattatct tgaccattta
     1861 tgtttattgt tccctgagtg gggagtactt ctttcaaatt caagttaaat ttatattcca
     1921 ttacttctcc tccaatatat aaaaattact taactgtatc caaatttaat
//
LOCUS       DAYYCY010000252         1965 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.259, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000252 DAYYCY010000000
VERSION     DAYYCY010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1965
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.259"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..415)
                     /locus_tag="ACHAZB_002725"
     CDS             complement(<1..415)
                     /locus_tag="ACHAZB_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM8615336.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKD"
     gene            complement(412..1065)
                     /locus_tag="ACHAZB_002726"
     CDS             complement(412..1065)
                     /locus_tag="ACHAZB_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8615337.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            1353..>1965
                     /locus_tag="ACHAZB_002727"
     CDS             1353..>1965
                     /locus_tag="ACHAZB_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HFM8615338.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCYDCGQTFSAKTDLVDE
                     HHQLTKELKQAILIELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFL"
BASE COUNT          618 a          350 c          252 g          745 t
ORIGIN      
        1 aatctttcgc atctttttgc atatcagctg tagaaacaaa ttgtagattt gtttttttac
       61 catcattaag ctttatttct ctttcttcaa ttatcaaaga attactatca ctttctaaat
      121 taacatttat attatctttg atttgtattt cattcttatt atttttttct tttatgaata
      181 cttttatttc actactcttg gaataaaact ccaaagtttg ttctatatat tttatttctt
      241 ttccattcat attactagaa atttcatttg ccatattatg aatcttttct tttctcgtct
      301 ccaaatatgt tttaggaaag ataaaaaaaa ttaatgaatg aactaatata attattattc
      361 caagaacaga aaatatttgt atgaacattt ttggaaatat ctttaatttt ttcattttct
      421 ctccaattta tatcctacat ttcttatagt agttatacag tctaattgta atttttttct
      481 aagttctttt atatatacat ctattactcg atcataagga gtttcttcgc tatctttcca
      541 tacataatca ataatttgca ttcttgttaa cacttgtcca ttattatcca ataaacattt
      601 taatacttct agttcttttg catttacatc tatttcttca ccatttattt tagctgtata
      661 gctattaaaa ttaaccgata gatccttata ttcaaacttc tctaaatgtc cataattctt
      721 cttaattaaa gaatctattc tagcttttaa gaccggcaat gaaaatggct tttctacata
      781 tccgtctact aaattagtaa atgcatcaat tttatattct tcatcactaa atgcagtcaa
      841 gattagaatt ggtaaattgc ttttctttct aatttctttc aacacttcta aaccatttat
      901 aaaaggtatc tgaatgtcta agataaccaa atttatatcg ctattaaatt ttgacaaggc
      961 ttctcttcca tctttagctt gaataacagt atatccaaat tctgaaagat attcacttat
     1021 tccctctctt atcatattat catcttcaac agttaatatt ttcatatata cctccactta
     1081 agcctataca ctagatatta tacagcatat tatggctcta taatatctag tgttggtgac
     1141 accttctgtt gccaatactt tttctttttt ctaataaatg caaacagaca ccttgaaatc
     1201 ctttagaata aagggttcta taatattttg tgttgttgga aaatcgaagg cgattttcta
     1261 ctaacacttt ttcttcgttt caactcaaaa agagactcac ttttggtaaa atttagtcga
     1321 ccaaaacatc accctaccaa aggagtcgct ctatggataa tcatactaga aaattactcg
     1381 gattaacaga taaacattta ttttttgatg aagaatggct gattgaaaaa gaatataaag
     1441 gcgttcaagc gcagtttatt aaagggagat tggatgactc accttcccat tgtgaacact
     1501 gtggatcgat tcgtattatc cgcaacggtt cctatacgac acgtactcag atattgaaag
     1561 tcaaagaaaa gttgactatt ttagaattaa aacgtacccg ttttctatgc tatgattgtg
     1621 ggcagacctt ttctgcgaaa acggacttag ttgatgaaca tcatcaacta acaaaggaac
     1681 tgaagcaggc gattctcata gaactgtacg aaaatcaatc acgtaaacta atcgctaaga
     1741 agtacttcgt ctcggatggg acggtgacac ggatcttgcg tgaagcaacg aagcattacc
     1801 agccacgtat gaacttcctt ccgacggtgt tgtgtatgga tgagttcaaa tcaatgaagt
     1861 ctgtttctgg ttccatgagt tttatttgtg ttgatgggac aacccatcag ctatttacga
     1921 tccttgaaga tcgcagactt tacaaactaa ctcaatactt cctgc
//
LOCUS       DAYYCY010000253         1936 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.260, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000253 DAYYCY010000000
VERSION     DAYYCY010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1936
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.260"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            102..524
                     /locus_tag="ACHAZB_002728"
     CDS             102..524
                     /locus_tag="ACHAZB_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8615339.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQQYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            complement(690..1883)
                     /locus_tag="ACHAZB_002729"
     CDS             complement(690..1883)
                     /locus_tag="ACHAZB_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8615340.1"
                     /translation="MTTYQRKVLVSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFVMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
BASE COUNT          687 a          407 c          327 g          515 t
ORIGIN      
        1 taataacagg tttatgcact attgacggtg tgacaatatg atatttgtat ccgttttttt
       61 cttgtatgta gtatagtaaa gtagatacag gaggagaaaa tatgcagact tttttaacaa
      121 aagtttctca aaagaaaatt aaaatgattc atttactgct tgaaacagaa cgttggtgtt
      181 caatagaaga actacaaaac gaattgaagg tatcaagtaa gtctatcttg aattatctaa
      241 cagatttaga agaattgttc caacaatatc ctgataaggt cattttgaag aatgagaata
      301 atcggcgctt ttctatggaa aagcaggaga acttcccaat ctacattatt tatcttcatt
      361 tctacagaaa atcatataat tatcatttga ttgaattcat gtatcaacat cctgagaaaa
      421 atttggaaga ttatgcagac gctcaatata cgagtgtatc cacagttttt cgttatgcta
      481 agttattagt aaagtacttt gaaatgacgg acgtcaaaga atagtcgccg ccagatagaa
      541 ggaaattagc agttgaaaaa gctgccctgc atcaagtaat acgaccagcc aaacggaaac
      601 agtgtctcat gtttttgatt tggctggtct tattaccgca gatgcaagct ttttccacac
      661 ctctttcttg tcatttacag actcaactat caatgctgca agctttccct taatgctgta
      721 acaccagtaa gagttgtcaa gaccagcaga ctgactaggt aaataccgct caaaaacaag
      781 attacggcag ttaaactata atgatccatc aagaaaccga tgacgataga agaaaaacca
      841 ccaacagctc gtccaaggtt cataacgaaa ttattcgccg tcgcgcggat ttctgttgga
      901 tacaaactac taatcactgc gccatatcca ccatacatgc cattgataaa gtagccgact
      961 atcacagcgg ctgctaccaa actccactgg ctatgggcta acacaagtag atagacagat
     1021 aaggatgaac aaattaaaaa caaagaaaag gctagtttag gccccaattt atccatgatt
     1081 atgccaaaag tcatcatccc caaagacatg cctaaaatag tactgaccat ccacaatgaa
     1141 gacccagaaa ccgaaagccc taatcgattt tgcataattg aaggtagcca attcattaac
     1201 ccaaaatagc cggcaatttg gacagttacc atgatactaa ggccacatgt ttgccaagca
     1261 agatgttttg attcaaacag tctgctaagt ttggttttct tttcggcaga ttgtacagat
     1321 ttgaattcct ttggttcagt caagccgcga cgaatgaaca agaccatcaa caccggcagt
     1381 acacctacga catacaacat cttccaacca aacgttggaa tgatcacagc agctaaaatc
     1441 gcagccaaag ccgcaccgac ctgcgcacca ataccagcaa ccgaagaagc tcgtccaatt
     1501 tgtttttttg aaaaactttc ggaaatcaag gacatacacg ccccatattc tcctcccgct
     1561 cctacacctg cgataaagcg aaagagataa accaagtaga tgtttgaagc aaaatacatc
     1621 aataaagaag caatcgaaaa cagaaggacc gtttgagaaa atactttcac acgtccatat
     1681 ttatcagcta tgatgccaaa aaaaatacct ccaatgagca tccccaaatt agtgatcgtt
     1741 gaaatgaaac ccgcttgcgt accactaata tgaaaagaag taatcattga agataatgaa
     1801 aaagctaaaa acataatatc catattctct aaagcaattc ccgccgatgt cgatactaat
     1861 acctttcgct gatacgttgt catttttata ccccttttcc taaaatttcc ctaaattgtt
     1921 aaaagtatat ccaaaa
//
LOCUS       DAYYCY010000254         1919 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.261, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000254 DAYYCY010000000
VERSION     DAYYCY010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1919
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.261"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(93..635)
                     /locus_tag="ACHAZB_002730"
     CDS             complement(93..635)
                     /locus_tag="ACHAZB_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HFM8615341.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            770..1159
                     /locus_tag="ACHAZB_002731"
     CDS             770..1159
                     /locus_tag="ACHAZB_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8615342.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            1163..>1919
                     /locus_tag="ACHAZB_002732"
     CDS             1163..>1919
                     /locus_tag="ACHAZB_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM8615343.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKK"
BASE COUNT          597 a          339 c          372 g          611 t
ORIGIN      
        1 atatgtcgat gttttttaat caaaataata aataaaaact gtctaacatt taaaatttaa
       61 tttagatatt agacagtttt taagtttgtg agttattttc cttgtcgttt tctcactttt
      121 ttagtccaga aaccacctga aatatatttt ggctcataag aaatgataaa agcacgctct
      181 tcgatttctt tgatcaggcg atacagttca cgttcgcttt ttctaggaga aagtatttcg
      241 agaactagcc gctcaccttc cagccccatt gcagaagtct gtgtcacacc ataaccgtgt
      301 tctctcagta tacccggcaa acttttatct tctgcgctat ttgctggcaa aatcgttgtg
      361 accatgatgt atcctaatgc caatttgtct tcaatcaaaa ttccgacact gatacctact
      421 gcgtaaccta acgcatagac aaataaattc agtggattat ctaaacgatc taaaacaagg
      481 gataagccga cgatataaat cgtgatctcg accatactga caagtggagc aatcagtcga
      541 tagcctttca ttgtcaacat gaaacgtaac gtgttcagag tgatataagc aaaattaatg
      601 aggaagatca tccccaacat ttttggatca atcatttgta aaactccttt catttatata
      661 acaacaatat caaaaaaaat cgtaagtgaa gaaaaattga aactttatac atacaaaaaa
      721 atgataaaat gattataata gaattataaa gataggttag gtgattaaaa tgaaaacggt
      781 caatcgtgtg ttttgtgcca gccacgtgct aagtctcctt ctcagtatat acttaggtgt
      841 ctattcgatt caacagccaa ttgaggtaga gaacattgtt tttcagatgt cgctgacaga
      901 tggtttaatt ttaagcgttt ttttcttttt gatactgttt attggtaata ttttcggtag
      961 cgttgccagt tttttgaatt tttctattta tccactcctt tcgctagcat taggagtggt
     1021 gggactttgt tctctgtgct tatttcccga gccattcagt cctgcattta tattatttgg
     1081 tacactgaac ctgttccaga caactgttgg agcatggttg ctatggcgat caggaaattt
     1141 aatgaaaata ggtgagtaac aattgaatcc aattatattg aaacgttctg tccatcatat
     1201 tccagtgtta gaagttgttc tgaaagaatt aagagaggaa cgtattcccg ttgtcatcta
     1261 ttatcacgga tggcagacaa gtaaggagtt agttttgacg caaggaagaa aaattgcgca
     1321 gcagggcatt cgtgtgatcc tgccagatgc gatgaatcat ggggagcgca aacagccggt
     1381 ttcttctatt ccatcctata cattttggga tagtatctac ggaaatctgt ttgaatttga
     1441 cacacttatc aagcatttag aaaaaagagg tcttctacat gatcagcttg cagttggcgg
     1501 ggtttcgatg gggggaatga caacatgcgc cttgttggct aaacatcctc atatagtagc
     1561 gggcgtgtgt ttgatgggtt cccctgcacc tttggaatat ggaaaagaaa tcgtaaggcg
     1621 cgcatcggaa tataattatg tcgtaccaaa agactatttt gatttgattt catgggttaa
     1681 gagctatgat ttgtcattgc agccggaaaa gttagctgga cgtcctctgt tcttttggca
     1741 tggacaagct gacgaaaaaa tcccttatat acaaacggaa cgatttgtag aaaaaaatcg
     1801 gcataagtct tacggaaaag aaattgtttt tcgttcctca aaaacacagc gacatatggt
     1861 acaaatacca ctaatggaag aagcttccac ttttttagca aaaaatttga taaaaaagt
//
LOCUS       DAYYCY010000255         1904 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.262, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000255 DAYYCY010000000
VERSION     DAYYCY010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1904
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.262"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..557
                     /gene="lepB"
                     /locus_tag="ACHAZB_002733"
     CDS             6..557
                     /gene="lepB"
                     /locus_tag="ACHAZB_002733"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFM8615344.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(640..1182)
                     /locus_tag="ACHAZB_002734"
     CDS             complement(640..1182)
                     /locus_tag="ACHAZB_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFM8615345.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(1419..1817)
                     /locus_tag="ACHAZB_002735"
     CDS             complement(1419..1817)
                     /locus_tag="ACHAZB_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8615346.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
BASE COUNT          668 a          339 c          314 g          583 t
ORIGIN      
        1 agagattgac aaaaaaacaa cgctatatta atcgcttctg gcttgtattc aaatacttac
       61 ttgtttctgt attccttgcg ttcatgttgc ggggattttt attcatcccg gtaccagtgg
      121 aaggcaattc aatggaaaac gtgttgaagc aaggtgatat ggttgtgatg gaaaaatttt
      181 cggagatcag acgatttgat attgttgttt ttcaattagc agatggcacg atttatatca
      241 aacgagtaat cggtcttcca ggagaaaatg tcagctacca aaatgatcaa ttgaagatca
      301 atgggaaagt agttaaggaa ccttatctaa ctaaaaacct gaaatcagat catgcaaatg
      361 catcctatac gacggacttt acactacaag agctaacggg acaatccaag cttccggaag
      421 ataattattt tgtacttgga gataatcgaa gagtttcaaa agatagtcgt tcgtttggca
      481 cgatcaataa aactgatatt ctaggtaaag caagatttgt ctattatcca cttgatgaaa
      541 taaaatggat ccagtgagaa acaataaagc gtgcagtctg ttgagactgc acgctttatt
      601 gtttaatcca aaatcagttt actaccttag tacgtaaaat caaacagctc gtttaaacac
      661 ttttctagcc tctaaagttt tgataaatga ataaacaacc attatttgaa tagggatttg
      721 gatcatattc ctgatgaatc tacctagaaa aattactttg aaatctgcac caaacaaaac
      781 agacatcagc caagaattga taaaaaggct gactgggatc ataatcaata attgggcagc
      841 gatgacggaa ggcaggttta tctttttatg atataaaaat aagccattaa taaatccacc
      901 aaggaaagcc gtgataagaa aagcagcttg aaacgttcct gaaccaaata tcgtgaaatt
      961 taaagtgttc gacaacattt caagcaaacc agcccagatc ggtccatata gataacccat
     1021 aagcgccata cttaaaaatc cgaatgtgat ttgaaaataa gcaggaccaa tcgctatctt
     1081 gtataagatg acatcaaggg ctaataaaag agcagaggtc gacaacaatc ttgcattcat
     1141 ttcgggcaat ctaaaaacgt cttttgcaat caaagtgttc aaaatattct ctcctttgtc
     1201 agttaataat aaaaggatac cataaaatca ccaatgtgtc atgacacatt ggtgatttac
     1261 atacactggc atctaacaat taaaaaaagg gtgagaagac tgcttttata aactgtaaaa
     1321 aagagattgt acgaataaaa aaaatagagg cggagaatac ttatcgtatc atccgccttt
     1381 gtttttttgt tataaaaaac gtcagattag ttatctgctt acatcttcat ccatacgggt
     1441 tctaatgtat ttccgtctag atcttggact tctaatgaaa acatttcttc ttcaggcatt
     1501 cccatatcta cttcgtggaa agtaccacca ttttcttttg caatttgagc aaattttttt
     1561 acttcatccg cgttttcaac actaaacgaa atcaaagcac cacttgtttt tttattatca
     1621 gctatttctt tatcttttat aaatttttta taaaaatcat gagagagaag cattatccaa
     1681 aaagtgtcat cccataccat tgaaccagct tcttcagtag aaaattcttc atttttttta
     1741 aaacccagct tttcataaaa agcaacggaa cgattgacat cagcaacagg aaaattaaca
     1801 aaaaccatac tattcatttg tttcgtctcc tttctgtttt gttcgtctat catctcttat
     1861 ttaggaaacg ctgtcaaaaa tttagattca tatatagaaa ccaa
//
LOCUS       DAYYCY010000256         1902 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.263, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000256 DAYYCY010000000
VERSION     DAYYCY010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1902
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.263"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..827
                     /locus_tag="ACHAZB_002736"
     CDS             <1..827
                     /locus_tag="ACHAZB_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HFM8615347.1"
                     /translation="YFKVDKKIKKLASTQQLQSRKSKLLFLALVFEDQSYVIIDQSGH
                     PVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIPVLIEEKRSGKELANCWV
                     GLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWMLPNEIRKKNTADSLVLT
                     KALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLLGSWQRATKRIREGKPVM
                     IGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYHKVIPASWSNGTVSLP"
     gene            907..1881
                     /locus_tag="ACHAZB_002737"
     CDS             907..1881
                     /locus_tag="ACHAZB_002737"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFM8615348.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
BASE COUNT          632 a          329 c          431 g          510 t
ORIGIN      
        1 tatatttcaa agtagataaa aaaattaaaa aattagccag cactcagcag ctgcagtcaa
       61 gaaaaagtaa attgcttttt ttagctctgg tttttgaaga tcaaagttat gtgattatcg
      121 atcaatcagg acatccggta gaatattctc cagcaaaata tacttatcaa gagggtcttt
      181 cttgtaaaaa atggaagctg atcaatgaag agccaatcga gttatctaga tggatcaaca
      241 gaaaagaaga tattcccgtt ttgatcgaag aaaaaaggtc aggaaaggaa ctcgcaaatt
      301 gctgggtggg attgccagaa gaacgttttt tgaggtataa gaaatgggct accccctcag
      361 gctatctttg tggtacttac gcggcagcag ttctccttgc ctattatcaa gactatcgta
      421 aggggtggat gcttccaaat gagattcgca agaaaaatac ggctgattcg ttggttttga
      481 cgaaggcatt gcgtagccag atccaaccac taggacttcc tacgattcct tttcaggtta
      541 gtacagggat ttctagtttt ttaaagaaga atggcaatca tgagcgtgca cgagcaactc
      601 ttctaggttc atggcagcga gctacaaaac gtatacgtga aggcaaacct gtgatgattg
      661 gtattttgaa agtacttggc agtacatatg gcaatcattg ggtgacagcg tacgcttatt
      721 ttgaaactga aacaggagaa cgttattaca aagtacacga caactggggc gattatcata
      781 aagtcattcc tgccagttgg agtaacggaa cagtttcact tccttaagaa tttgctgaaa
      841 ttaacagaaa atcttggaaa aatatgttaa actatcgaag aatagagaag ttttggggag
      901 gactacatga gtaataagta tcaggatgac acgttgaaga tttttagttt gaatggcaac
      961 cgtccattag ctgaaaaaat cgcgaaagtg ttcggaacag aattaggtaa aagcgtcgtg
     1021 aagcaattta gtgatggcga gatttccatc aatatcgaag aaagtattcg aggggatcat
     1081 gtgtatatcg ttcaatcaac gaatgcacct gtcaatgact actatatgga actactgatc
     1141 atgatcgatg caatgaagcg agcaagtgcg aaaacgatca atgtcgtatt gccttactat
     1201 gggtatgcta ggcaagaccg cacggcgaaa cctcatgaac cgatcactgc gaaattgatc
     1261 gctaacttga tcgaagaagc aggggcaact cgagtactga ctttagatct gcacacggtt
     1321 caagtacaag gattctttga tattcctgta gataatttat ttacgatgcc gttgtttgct
     1381 cattattacc gtcaattagg tctgacggga gatgacatcg tagttgtttc gccaaaaaac
     1441 agcggtgttc agcgtgcacg tagtctttca gaatatttga acagcacatt agccatcgta
     1501 gatcatgcag atgatgcttc aactgagggt ggttatgtga tcggtgatgt tcaaggaaag
     1561 acatgtatca tggtagatga tatcttgaac acaggagcaa cgcttgcacg agcagcaaat
     1621 gtattaaaag aaaacggcgc aaaagaagta tatgcctgtg cgtcacatgg attattgtcc
     1681 gatcatgcga aaagtatttt agacaatgca ccaatcaaag atatttgtat cactgattct
     1741 gttttcacag aagaaaatcg tcatccagaa aacttaacaa tcgttacatg ttcagaatta
     1801 atgggtgaag cggtcaaacg catccatgaa aatacaccga tgagtccgtt attccgtctg
     1861 gaagaaaaag aattcgaata aacaaaaaaa agagggaaag aa
//
LOCUS       DAYYCY010000257         1901 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.264, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000257 DAYYCY010000000
VERSION     DAYYCY010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1901
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.264"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            47..1188
                     /locus_tag="ACHAZB_002738"
                     /pseudo
     CDS             47..1188
                     /locus_tag="ACHAZB_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(1314..>1901)
                     /locus_tag="ACHAZB_002739"
     CDS             complement(1314..>1901)
                     /locus_tag="ACHAZB_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM8615349.1"
                     /translation="AMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFV
                     NTFGLVLEAQFVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPL
                     SFIAYSQGGIRDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFF
                     GVWGLIVGIPIFTFFLDVLKVKKIPNHLPKETDIP"
BASE COUNT          772 a          370 c          329 g          430 t
ORIGIN      
        1 actatcttat ttcttgcata caaaaaaatc aaggagtgag agagttatga gatattcaac
       61 catcttaata tcaacatttg cagccttaag cttatcgctt actataaatc agacagtcca
      121 cacagaggag acagaaacag ccaaatggac agaaaccaag tgggccaacg tcaatcgaat
      181 tacctttcag gatgaatttt catccttcaa ttcaggagtc gcaaaattaa gaaaaggaca
      241 aacagcgacc atcaaagcaa aaattgacta ttcgggagac ttagcaccta tactacaatc
      301 agctgttaca tttgagaatg ttgatcccac tttgtcgatt ggctatgacg agaacacttt
      361 gactatccaa cacaagcaag cgatatttac cttcaccgtt acattaaatc aggacttgtt
      421 aaaaccagcg ctttttactg tcaaggtatc agatggacta ccgaacgatc atcatttaac
      481 gccgtatagt cagcggcaga caattgagca agattcagcc atcgactcag gaggagatct
      541 tgttgaagaa ccaacagata aaccagaaaa tgaaaataag ccagaagtac cgcctacaga
      601 aaatccagac ggagaacaga aaccggaaat agaacctgga gaagaacccg atacagaaac
      661 tcaaccagag ccagacaata aatcaaaacc tgaaataacc cctggagaaa aaccagatgt
      721 aactccagaa ccagatacgg attctggaaa ccaaacagta ccggaaacaa atccagacac
      781 agataatgaa actgaaaatc cagaaaaacc agaggtagat ccagaagaaa aaccagatgt
      841 gactccagaa ccagatacgg acgcaagaga ccaaggaata ccagaaaaaa taaacaaaaa
      901 aacaattcaa gaggatggaa aaaaagagag taaaaaatca aatctagcaa ttctaaaaat
      961 aaatgaagag cagctgaata aaaaatcacg aatatttgat agcgcccaat cagctgaaac
     1021 cttaaaaagt agcaaggata caacctttgc tgtcctgaaa cgaaaaataa acagctacca
     1081 aaatccggag agtcacaaaa caaagttata ctgtggagcg gaatcattct attaagtata
     1141 gcaacaatgc tttctgcaaa aagatttaaa caaaacagat ctctctaaca tttgaataat
     1201 gtctgtgaac gcatttttca aataaaaaga aatatccaga aaatgacccc aaaataccag
     1261 attgaaatct gatatttggg gtcgttcata ctttatcttt cttcatattt attttaaggg
     1321 atatctgttt cttttggaag atggttcggg atctttttca ctttcaatac atctaaaaag
     1381 aaggtaaaga tcggaatgcc gacaatcaag ccccaaacgc caaagaaccg ttcgctaaca
     1441 agtaagacca caaatgtata aaaaatcggc agttctgttt tgctggacat caacttcgga
     1501 ttcagcacat aagactcaat tagatggaca atcaaaatcg taatcaggat ataaacaaca
     1561 tctcggatgc ctccttggga ataagctatg aaagatagag gaatacaaga aatgataaca
     1621 cctgctactg ggatcagact taaaatgaaa atcatgatcg ctaaactcag cagctgatga
     1681 aagccaaata cagccaaggc gattgtagtg agcaacgtat ttaaaagagc gacaacaaat
     1741 tgagcttcta ataccaaacc aaatgtattg acaaacttat cggcaaaata gtaaatatct
     1801 tgaaagaacc aagaaaattc actttttaaa aacaatcttg agaatacaat tgtttttttc
     1861 ttttcaatca taaaaaagaa acttaagatg aatgacatag c
//
LOCUS       DAYYCY010000258         1898 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.265, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000258 DAYYCY010000000
VERSION     DAYYCY010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1898
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.265"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            5..1150
                     /locus_tag="ACHAZB_002740"
     CDS             5..1150
                     /locus_tag="ACHAZB_002740"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFM8615350.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            1321..1779
                     /locus_tag="ACHAZB_002741"
     CDS             1321..1779
                     /locus_tag="ACHAZB_002741"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0043768 - S-ribosylhomocysteine lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFM8615351.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
BASE COUNT          621 a          357 c          377 g          543 t
ORIGIN      
        1 aaacatgcac attcaaacaa aattgatcca cggaggcatc agtgaagatc ccacaacagg
       61 agctgttagt gtacctattt atcaaacatc cacttatcgg caagatggcg tcggccagcc
      121 taaacaatat gaatattcaa gatctggtaa tcctactcgt tttgcattgg aagaactgat
      181 tgctgatttg gaaggcggtg ttcgaggatt cgctttttct tctggtcttt ctgggattca
      241 tgccgttttt tctctttttc aagctggcga tcatatctta ttaggagatg atgtctatgg
      301 aggaacattc cgtctatttg acaaagttct tactaaaaat ggtctcgagt acacgatcat
      361 agatacgagt aatcttgata aaatagaaca atccatcaaa cccaatacaa aagcacttta
      421 tcttgaaaca cctagcaatc cgcttctaaa gattactgat ttagaaaaaa gtgccactct
      481 ggctcatcaa catggattga tagttattgc tgacaataca tttgctactc cttattttca
      541 aagaccttta gatctcggtt ctgatatcgt tgtccatagc ggtaccaaat atctaggcgg
      601 acacagcgat gtagtagctg gtttagtgac ttctaaccat aaagatctag ccgatcagat
      661 cggattttac caaaatgcta tcggagccgt acttggtccc caagatagct ggttacttca
      721 gcgcggaatc aagacactaa gtgttcgtat ggaggaacat caaaaaaatg cttttgtggt
      781 agctgatttt ctattttctc atccagcagt agaaaaggtc tactatccag gattacccga
      841 tcatgaactc catggagttg ctaagcagca gatgagcggc tttagcggaa tgatttcttt
      901 tactttaaaa aatgaagaat ctgctattcc atttgttgaa tcactccaac tatttacttt
      961 agcagaaagt ttaggtggcg tagaaagttt agtggaaatt ccttctgtca tgactcatgc
     1021 gtctattcca aaagaaaaac gagaagaggc tggcataaag gacggcttga tccgtctttc
     1081 tgtaggaatt gagtatggtc aggatctcat aaacgattta gctcaggcat ttgacagaat
     1141 caagaactag aagtcccaat aaacgactta gcagaaaaat ctaaaaaata tttcagattg
     1201 ctacaccttg gaaacgataa gtgttagaat caagtagaaa gacgtttttg taaatttcac
     1261 taaataattc tttagtgaaa agcatcaaaa cgaatgaaca aacagatagg agtcttacaa
     1321 atgagaatga atgtcgaaag ttttaatctt gaccatacaa aagtcaaagc accttatgta
     1381 cgtgtagctg atcgaaaaac gggtgcgcat ggcgatgtca tcattaaata cgacgtccgc
     1441 tttaaacaac caaataaaga acacatggac atgccaagtc tacattcact tgagcacttg
     1501 actgccgaac taatccgcaa tcacgcagat tacatcgtag actggtctcc aatgggatgc
     1561 caaacaggct tttacttgac tgtattgaat catgaaagct acgaagacat tttagatgtt
     1621 cttgaagcaa caatgaaaga tgttcttgaa gctactgaag tacctgcaag caatgaaaca
     1681 caatgtggct gggctgccag ccatacacta gaaggtgcac aagctttggc tcgtgaattt
     1741 ttagcaaaac gcgatgaatg gtctgaagta gaagcatagt ttctaaaaga acgtaaagat
     1801 gagaagcaat atagctatct tatcttcgta ttttattaaa tatttattgt ctgaaaagag
     1861 ttctaaacgg gaaaacaaat agctagtttt cagtttag
//
LOCUS       DAYYCY010000259         1833 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.266, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000259 DAYYCY010000000
VERSION     DAYYCY010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1833
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.266"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..969
                     /locus_tag="ACHAZB_002742"
     CDS             <1..969
                     /locus_tag="ACHAZB_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HFM8615352.1"
                     /translation="PINTFAYTINDEYNLAPNQGDSRLAIPNKIILHETGIDAPARNV
                     AANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAGNANPYAPVQIELQRTYDKA
                     LFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIISHLWVTNYVWGNHTDPYGYL
                     SQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNHAHIDQFGE
                     IENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYH
                     VSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
     gene            1159..1232
                     /locus_tag="ACHAZB_002743"
     tRNA            1159..1232
                     /locus_tag="ACHAZB_002743"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1193..1195,aa:Ile,seq:cat)
BASE COUNT          605 a          317 c          346 g          565 t
ORIGIN      
        1 cccattaata cgtttgctta tactattaat gacgagtata atttagcgcc gaatcaagga
       61 gactccagat tagcaattcc taacaaaatt attttgcacg aaactggaat agatgcacca
      121 gcaagaaacg tagccgccaa catgaaaaat aattataacg gaagcaattc ttatactaca
      181 gatgttattg gtgacggtgg gattgtttac cgtgtgggtg agcaaggata tgtttcgtgg
      241 ggagctggta acgctaatcc ttatgcgcct gtacagattg aattacagcg cacatatgat
      301 aaagcattgt ttgaaaaaaa ctatcgagct tatattgaat atacaagaga tagtgcaaaa
      361 aaatatggaa ttccattgac tcttgatcaa ggaacttctt tatttacaaa aggaatcatt
      421 tctcatttgt gggtgacaaa ttatgtttgg gggaaccaca cagatccata tggttactta
      481 tcgcaaatgg gagttagcaa agaaaagctt gcttatgatt tagctcatgg atttaccgat
      541 gaaaatccaa caacttctga aaacaagcct gtcattgatc caacacgagc tggtgcagct
      601 aatcctacgc tgacagatgg aacaaatcac gcccacattg atcagtttgg ggaaatcgaa
      661 aacgcaaact tgcatgtggc tggatggcac attgctaact ataaatacga gtatattttc
      721 attatggact acaatactgg gaaagaatta gctcgagtaa gagctgatgg aatttataga
      781 ccagatgtaa accaagctta taatacttct ggaaatgttg gttatcatgt atctttcaat
      841 atgcgtaatt ttcctagtaa gaaagtctat gtaatgatgc gggcaacgaa tgatccagag
      901 ggaaacacta aaggcggtgc gcaagatttc catgacaaac gttggtattt aaatattcct
      961 aaacgataaa aatagctcct cgttgaggag cagtacataa ctatattgac aactataaaa
     1021 attattcgat aaaatagtga tgttatcgca tatcttcact atcacccata aatagtcaca
     1081 ctccaagcta tgcgataaca ggtttgttgc cacacattct actggttgat tgtttatggc
     1141 tttatgtggc aacaaccagt acccttagct cagttggtca gagcagacgg ctcataaccg
     1201 tccggtcgta ggttcgagtc ctacagggta cattaacgta gccatttgaa tcgttctgtg
     1261 ttagaatttt tttgaagagt attatacaag ctaaagcttt tcttcattgc cactcaaatg
     1321 agtggctttt ttatgtatcc ttttatggat taatgaaagg atgtttcaca tagttatact
     1381 tctgtatatt tgaaaagttt tactttgatt tttaaataga aagacatttg ggttaaattg
     1441 tgagataata ataaagaaga gtttaaagcg caccccaaac cacttcccca taagtgtgtt
     1501 acgctttaaa ctcttttata tttgaagcca ttaaaaagca taccatattt ttgaaaaaaa
     1561 gtgagaaaaa aggcttacaa ttggagtggt agttaattag tgacttattt ttgattttat
     1621 agcactgata ctataaaata tagatatcat catattacac aatcttaata ctaacttaaa
     1681 aaatatctcc tttcacaagt atggtgataa aattcgttcc gggctacctt tttaggtagc
     1741 ctactttaat ctttatacct ttctggatca acgaaagtat actttatata gtcataacgc
     1801 cgatgatcgc tacgtgcgtc cggcacgtca gtc
//
LOCUS       DAYYCY010000260         1802 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.267, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000260 DAYYCY010000000
VERSION     DAYYCY010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1802
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.267"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            298..495
                     /locus_tag="ACHAZB_002744"
     CDS             298..495
                     /locus_tag="ACHAZB_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8615353.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            601..1083
                     /locus_tag="ACHAZB_002745"
     CDS             601..1083
                     /locus_tag="ACHAZB_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8615354.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(1141..1737)
                     /locus_tag="ACHAZB_002746"
     CDS             complement(1141..1737)
                     /locus_tag="ACHAZB_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFM8615355.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
BASE COUNT          548 a          270 c          360 g          624 t
ORIGIN      
        1 catttgaaat ctcccttttc agctgtttat ctttcctatt ataaatgtat ttttattgga
       61 aatggaagta ttatatttta atatgtagga aataattagt ttttaaggaa tatgttttaa
      121 ttttttgaaa aaaattcttc taaatacaac agctgtttta attgtgaaaa cgaaaacagt
      181 acgctataat gcctgtaagg ttgattttta actgtaaata aaaatcaatg agttaaatct
      241 ccaaagaaat agcagtggta atgtggagaa aagttccagc agtttaggag gattttgatg
      301 acggaagaaa tgattaattt aggagaacaa tatgcatgca aaccaattgg ttttacaaaa
      361 acggtcattg gtgaagttgt atcaaaaatg acaaactgcg ccgtggtcaa agttgcccaa
      421 tgtgctgcag aggaccaaga attattagat gaaaaagcaa gcatggttgt tgcaaaatat
      481 gatacatttg aataaagaaa acaattggac gtgcggcgtg ctgcaagtcc aatttctttg
      541 agaaggtaaa tcagtattct tttcctattt tctggagaag aaaaagcgag gcgagttgtc
      601 atggtaacat tctttacggt tctattgttt ttattcgttg ttgtgatgtt tttatttctt
      661 tggctggttc gaaaagaaat tatttatcgg actgtgcgaa atcgatgggt ctacttggtg
      721 atacccttct tagtgtcact agtcatttgg tatacactca tcagtcatcc tacagcagat
      781 gaattggcaa aagggatctt gtcagcaatg atttttatta gttttttgtt ggacagtaga
      841 ggaatcacag aagaaggact tgttttgaat agctttgaca aaaaaggtgt tcctttttct
      901 gagatcaata aaattgtatt atatcagcca aaaggttcta aaatcgtaaa aatgaatttt
      961 ttccgaaacg gatggcgagg accgatgctg aaattttctg catcattaga agaattagtt
     1021 ccatttttgt cccaacgctt aaacgatgag gcagagattg atattatgat tgatccagaa
     1081 taaaaacaga ggttgcgaca agatgtttgt cacaacctct gttttttatt tcaaataaaa
     1141 ttatccaaat acgcggaagc gatcggcatc gttcatgtat tctttttccc cagccggtgt
     1201 ttctggtgaa ccgaatacta attgcgaacg tagtttccag tttgaaggga tattccattc
     1261 ttttgcaact gcttcgtcaa taactgggtt gtaatgttgc aagtttgcgc ccaatccttt
     1321 atcaactaat gctacccaag tattagctgt tgcgattccg tttgattgtt ctgaccaatc
     1381 tgggaagttg tctgcatata attcaaattg ttcttgtaga ccttttacca cgtcagtatc
     1441 tttgaagaat aatactgtac cataaccatt tttgaaacca gctaatttgt tttgtgtatt
     1501 tgggaaagct tctgctggag tcaatgggcg aagtgcttct tcagtgattt cccaaagttt
     1561 ttcatgtgca tctccgaaaa ggataactgc gcgtgttgat tgagcattga aagcagttgg
     1621 gctttcttta attgcttctt tgatcaaagt tgttaattct tcattagaaa gtgaaacatt
     1681 acgtcctaga tgatagattg aacggcggtt tttcaaagtg tcagtaaaag ttgtcataat
     1741 aaaatttctc ctttgatttg cgttttgcat ttttttaatt gattacgaaa tgaagtatag
     1801 ca
//
LOCUS       DAYYCY010000261         1799 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.268, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000261 DAYYCY010000000
VERSION     DAYYCY010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1799
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.268"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            29..1381
                     /gene="brnQ"
                     /locus_tag="ACHAZB_002747"
     CDS             29..1381
                     /gene="brnQ"
                     /locus_tag="ACHAZB_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFM8615356.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT          506 a          309 c          353 g          631 t
ORIGIN      
        1 ttttaaaaaa accgtaaagg agttatgtat gacaaaaaaa ttatcattta aagactatct
       61 ttttataggc tccatgcttt tcggtctatt ttttggtgct ggaaatctta tctttcccgt
      121 tcatttgggt caagaagcag gcgctgctac cttttgggcg aatctaggtt tcttggttac
      181 cggtatcggt cttcccttct taggggtgat tgccattggt gtatcgaaaa gctcaggtgt
      241 atatgaatta gcaaaacgga taaatcaaac atatgcgatg gtttttacca tcttgcttta
      301 cttagttatc ggaccgtttt ttgctttacc tcgccttgca accacatctt tcgagattgg
      361 tttagcacca tttatcgatc ctagcagaca gacgctatat ctagcaattt tcagtattgt
      421 attttttgtg ctggcttggt ggttttcaag aaaaccgaca aagatattag attatgttgg
      481 aaagttcctg aatcctgcat ttttggtttt attaggtgct ttgcttctcc ttgctttttt
      541 gaatccacta ggttctgtaa atcaagcgcc tattcagcca aattatcaag agcatgcttt
      601 tttcacaggg tttactcaag gatataacac attagatgcg cttgctgctt tagcatttgg
      661 gatcatcatt gtcacaacaa tccaaaacat gggtgtaaca aaacctgctg aaatcgctaa
      721 agacacgatc aaatctggtg cgatcagcat tatattgatg ggaatcattt atactttgct
      781 cgcttattta ggtgctatga gcttaggtag ctttgctcta agtgaaaatg gcgggatcac
      841 gctggctcag attgctgatc attatttagg tacttatggc agtatcttat tagcatttat
      901 cgttatatta gcttgcttga agaccggtat tggtttgatt acagcttttg cagaaacatt
      961 cacagatttg tttccgcaaa gaaattacgc attctttgtt gcacttgcta gtatccttcc
     1021 ttgtttagcg gcaaacgtag gcttgacgaa tatcattcaa ttttcgttgc ctgttttgat
     1081 gttcatctat ccattagcaa tgacactgat tttactagtg ttagttggtc ctttgtttaa
     1141 gcagcgtacg gctgtttatc gaatgactac ttattttaca ttgattgctt cgatttttga
     1201 tgggttgaac gcttgcccag aaactatcaa acaaacacct attgttcaaa atcttttata
     1261 tgttgcggaa agctacttgc cgtttttcaa attggggatg gggtggatcg taccagcagt
     1321 tatcggtttt gttatcggac tgatttggag tttcgctaag aaagaagaag tagcagattg
     1381 ataaaagagg ttgtgaaaga gacgttcagc cttgagcaat aaaaagtaat tttgaaaaat
     1441 ggcttctcac atttttgcat aaatttgact taataccgag aaactgactt ttgaacaccg
     1501 tttattgaga ggtcgtgaaa aagccgctta gtttcaagta ttaagaaata ttttgtgaaa
     1561 atggcttctc acatttttgc ataaatttga cttaataccg agaaactggc ttttgaacac
     1621 cgtttattga gcgtctataa actgacctga gtattagaga aacaaaacgg caaagtagct
     1681 ccatatactt tgccgttttg ttttgaacta ataccgaaga gaagctgatc aaacaacatt
     1741 tcttttataa tatagatttt agacatatat atgatataaa aatagttttc gcaaaaaaa
//
LOCUS       DAYYCY010000262         1755 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.269, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000262 DAYYCY010000000
VERSION     DAYYCY010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1755
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.269"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            60..746
                     /locus_tag="ACHAZB_002748"
     CDS             60..746
                     /locus_tag="ACHAZB_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HFM8615357.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            748..1692
                     /locus_tag="ACHAZB_002749"
     CDS             748..1692
                     /locus_tag="ACHAZB_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HFM8615358.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
BASE COUNT          482 a          284 c          427 g          562 t
ORIGIN      
        1 ttttttatgt gttttgcttt ataattaaaa aaatattgtc attttatgga ggaaaatata
       61 tggaaaatta tcttagcctt ttaggcgtct tgattgttat tctcgggttt gcatttaaat
      121 tagactcgat attgatcgta atggttgctt tagttgttac agctttgact ggcggtcttg
      181 gcattcaagg aatgttagaa cttttaggta caagttttgt aaacaatcgc gggatggcga
      241 tttttatcat cattatgttg gcaacaggaa cgcttgaaag aaatggtctg aaagaatcag
      301 cggcttcctt gatcaagcga ttcaagaaag tatctgctgg tatgattgta gatatttatg
      361 ggatctttcg aatgatcttt gcggctttta acgtgagttt tggtggagtt gctggatttg
      421 ttcgtccaat cattttaccg atggcattgg ggaccatcga atcaaaaggg ttagaagtcc
      481 atcctaagca tgaagaagaa ctaaaaggta tggcttcagc tatggaaaat gtctgctggt
      541 tctttggcca agtactattc atcggaggag caggggcgtt gctagtccaa tcaacactga
      601 aagatctagg atatgacgta acattgggac agcttgcttt agtggaagtt ccagtggcta
      661 tcacggcttt agttgtggca agtgtctact tctatcttaa agaaaagaaa ttagcgaaga
      721 aatattatcc aggaaagggg caataagatg gactttttta caaatcctga tattttatta
      781 ggcgataaat tactggagat cctttatatc gtgatgggac tcgtattgat ttacactgga
      841 gtaagaaatt taacagataa aaccaatcct catcgttatg ggtcagcctt tttctggacg
      901 gtcttagggg tagttattgc aggaggaaga tggcttccgg caatcgtgaa cggtggcttg
      961 atctttgcga tgaccatacc agcaattgct aagaaagtca gcaaaggaga aagccggctt
     1021 ccatcaaaag aatatatgga aaaaatgtca gacaaactag gaatgaaaat ttttattcca
     1081 gctttgagta tcggtgtctt tgcgatcttg tttgctctat ttaccaattt aggtgcttta
     1141 gtcggcgtgg gcgtcggtgt ttttgcagcc atgctaatct tgatgttctt ttctcgagat
     1201 aatcgaccaa gtacattttt agatgatgca gcagatatgc tgggcacagt agggccgtta
     1261 agcatgttgc caatgctttt agcttctcta ggtgcggtat ttacaagcgc tggagttggg
     1321 acagtgattt ccaatgcagt aggtacgatc gttccagaag gaaatgtgaa catcggcatc
     1381 atcatttacg ctcttggcat ggtgatattt acggtaatca tggggaatgc atttgcggcc
     1441 atcaccgtga tgacggttgg gatcggtgca ccattcgtat ttagtcatgg agcgaatcca
     1501 gcgttaatag gaatggttgc attgacttgc ggtttttgtg ggaccctgct gacaccgatg
     1561 gctgctaact tcaatatcgt gcctgtagcg atgctggaga tgaaagacaa atacggagtg
     1621 atcaaaaacc agttgtttat tgcgttattt atgctagttt tccaaattgc ttatatgatt
     1681 ctatttaaat aatagaagag aggggttgtg acagaataat tgtcgcaacc tcttctaaca
     1741 ttgtagtgga acact
//
LOCUS       DAYYCY010000263         1672 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.270, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000263 DAYYCY010000000
VERSION     DAYYCY010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.270"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            75..1622
                     /locus_tag="ACHAZB_002750"
     CDS             75..1622
                     /locus_tag="ACHAZB_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFM8615359.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
BASE COUNT          560 a          305 c          343 g          464 t
ORIGIN      
        1 tgaaccccta tttcttgtgc gtttcttgtg gtattctgta acaaaatcag ttcgattcaa
       61 ggtggtgaaa aattttggaa actacagata cgttgcttca gttgcttcaa gaagcccata
      121 aaaccaatca agcacaacaa caaaccattc agaatcttac aactgaaatc caactattga
      181 atgaaaaggt caattattta accaacaaat tatttggacg ctctaaagag actctctttg
      241 aggaaacgaa tgggcaattg aatcttttta gtgatgaaga aatttctgta agtgttccag
      301 aagcagctgc caccattatt ccagtaaaag gacatcaacg agttgtagga acgaaagcag
      361 acaaaatcaa acacttacct ataacagaga aagaacatct ccttcctttg gaagaacaat
      421 tttgtgaaca ttgcggttca cagatgaaag atatcggacg gacaaaagta cgagaagaaa
      481 ttcgtttcca tcaggctatg ttggattgtc tcacccatta tcaacatacc tattgttgta
      541 aaagctgcga aaaagaagga ctttcttctt ttaaaaaagc gatcgttcct aaacctttga
      601 tttctaatag cttaggttcc aatagtttag tggcggaaac gatccggatg aaatttggtc
      661 agaaagttcc tgcctatcgg caagagaact attggaaaca aacacatggg ttggatattt
      721 cgagagataa tatcaccaat tggcatatta aagcagtgca aaacgcgtta gaccctttag
      781 gagaacgatt aagagtctat cttaatcaag aagagattct acacggggat gaaacaagtt
      841 atcgtgtgat tgaaagtgcc aaaacagata cctattactg gcagttttgt acaggaaaag
      901 acagtcagca tcccatcgtt tattatcacc acgacgaaag tcgtgctgga gatgttccga
      961 aaacttttct caaggagttt acagggtatt tacactgtga cggctatagc gggtataatg
     1021 ccgtggaaag tgtacgactt gtctattgtt tcgcgcatgt tcggcggaaa tttttcgaag
     1081 caattccaaa aggaaagaaa aacacggata ttcccgcagc tcaagcagtc aaacaattgg
     1141 ataaatggtt tgtcctagaa aagaaatgga aagacttttc tcctgagaag cgattaagct
     1201 gtcggcaaca agaactgcgt ccattattta ttgcttttta tgagtggatg gcaacgatag
     1261 atcctgttgc aaaatccaag ttggatgcag ccgttcaata tgcgtgtaaa ctacgaagtg
     1321 gttttgaacc tatttttgaa gatggtcgtt tagaactcac aaataatcgt gcggagcgaa
     1381 atatcaaaga gctggtgata ggacggaaaa attggttgca ttccacaagt cttgaaggag
     1441 cacgaacttc aggaatcatt ctgagtgtat ataaaacagc agaacttaac ggattgaatc
     1501 ctgtaaagta tctagagttt ctatttgata agataccaaa tcttcccgta ctttcagcag
     1561 aaactttaga tcaactgttg ccttggaaca aggatgtaca acaacatttt tctcgtaact
     1621 aaaaacgcca aacattcccg attctattct ataggtcggg ttatttgacg ta
//
LOCUS       DAYYCY010000264         1664 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.271, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000264 DAYYCY010000000
VERSION     DAYYCY010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.271"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            131..505
                     /locus_tag="ACHAZB_002751"
     CDS             131..505
                     /locus_tag="ACHAZB_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFM8615360.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="ACHAZB_002752"
     CDS             complement(619..1248)
                     /locus_tag="ACHAZB_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM8615361.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="ACHAZB_002753"
     tRNA            complement(1382..1454)
                     /locus_tag="ACHAZB_002753"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
BASE COUNT          584 a          240 c          283 g          557 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt actt
//
LOCUS       DAYYCY010000265         1650 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.272, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000265 DAYYCY010000000
VERSION     DAYYCY010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1650
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.272"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            60..1088
                     /locus_tag="ACHAZB_002754"
     CDS             60..1088
                     /locus_tag="ACHAZB_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFM8615362.1"
                     /translation="MNSKKLQMGEIVRGPSQISIDVTYKCNFRCKHCYNASGGDNCFM
                     DNELTDDELLELTKDICNLKPYNICFCGGETLLRKKIVLSCATLLKEAGIPNIAIVTN
                     GSLVTQDVAKELFKAGINRFQVSLDGKNASSHEALRLYPNSFEKAINAIKLFRELSEE
                     VDVAFCPTSFNYNQLREVYQLVNTLKVKSLRIQPFMLSGRGMQNREIVPTVSQYRKLV
                     SDINYIKRNEDYTKIDWGDPIDHIIRHRTLLKDFSNFVSIRSDGGLMASPYLPIQVGN
                     IKCHSLSKYWRAGLYKVFSLDLVQDIATQVSSIQDMSKELKKGHIKWLNEDIEIDILD
                     EYKHDILI"
     gene            1144..1362
                     /locus_tag="ACHAZB_002755"
     CDS             1144..1362
                     /locus_tag="ACHAZB_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8615363.1"
                     /translation="MISEPQMNNMYESPTIMGAGEAAPRACSAIMVCVLLASAGVGIQ
                     AGAVYTYAVVATVAVAAAAVWKTVGLVG"
     gene            1400..>1650
                     /locus_tag="ACHAZB_002756"
     CDS             1400..>1650
                     /locus_tag="ACHAZB_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8615364.1"
                     /translation="MIKLRRNFKKIMKYNNLKPKLLNENIEYIREENYGYLTIVPKNG
                     TELKDNVFNPTVRYILANCTGKNSVKKIANGLKKKFPSL"
BASE COUNT          621 a          189 c          314 g          526 t
ORIGIN      
        1 agataatttt ttggattcaa atgatgaaat attatattta aataaggagt atgataataa
       61 tgaatagcaa aaaacttcag atgggtgaaa ttgttagagg tcctagtcaa atatctattg
      121 atgttacata taaatgtaat tttagatgta aacattgtta caatgcgagt ggtggagaca
      181 attgttttat ggataatgag ttgactgatg atgaactgtt agaacttaca aaagatatat
      241 gtaatttaaa gccatataat atttgtttct gtggaggaga aactttactg agaaaaaaaa
      301 tagttttatc ttgtgccact ttattaaaag aggcaggtat tcctaatatt gctattgtta
      361 ctaatggatc attagtaact caggatgttg caaaagaatt attcaaagca ggtattaatc
      421 gatttcaagt aagtttagat ggaaaaaatg ctagtagcca tgaagcactt agattatatc
      481 caaacagttt tgaaaaggca attaatgcta tcaaattatt tagagaattg tccgaagaag
      541 tggatgttgc tttttgtcct actagtttta attacaatca attacgagaa gtatatcaac
      601 tagtaaatac tcttaaagtg aagtctttaa gaattcaacc atttatgctt agtggtaggg
      661 gaatgcaaaa tagggaaata gtgccaactg tttctcaata tagaaaacta gtttctgata
      721 ttaactatat taaaagaaat gaagattata ccaagataga ttggggagat ccaattgatc
      781 atatcatccg acacagaact ttattgaaag atttttcgaa ttttgtttct attaggtcag
      841 acggaggatt aatggcatca ccttacttgc caattcaagt tggaaatatt aaatgtcaca
      901 gtctttctaa atattggcga gcaggacttt ataaagtttt ttctttagat ttagttcaag
      961 atatagctac tcaagtttct tcaatacaag atatgagtaa agaattaaaa aaaggacata
     1021 ttaaatggct taatgaagat atagaaattg atattctcga tgaatacaaa catgatattt
     1081 taatttaggt ttatcattgg aaagtttaga taaacaaagt acttttagaa aggagggaaa
     1141 aaaatgattt ctgaacctca aatgaataat atgtatgaaa gcccaactat aatgggagct
     1201 ggtgaagctg cgccaagagc ttgttcagca attatggtgt gtgtgttatt agcatctgca
     1261 ggagtaggta tccaagcagg agcggtgtat acttatgctg tagttgctac agtagctgta
     1321 gctgcagccg cagtttggaa aacagtagga ttagttggtt aggaattaat aggagactaa
     1381 ctgtctccta ttgattccaa tgattaagct aaggaggaat tttaagaaaa taatgaaata
     1441 caataattta aaaccgaaat tacttaatga gaacatagaa tatataagag aagaaaatta
     1501 tggatattta accatagttc ccaaaaatgg aactgaattg aaagataacg ttttcaatcc
     1561 aacagtaaga tatatattgg ctaactgtac tggaaaaaac agtgtaaaaa aaatagcgaa
     1621 tggtttaaaa aagaagtttc ctagtttaga
//
LOCUS       DAYYCY010000266         1607 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.273, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000266 DAYYCY010000000
VERSION     DAYYCY010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1607
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.273"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            44..466
                     /locus_tag="ACHAZB_002757"
     CDS             44..466
                     /locus_tag="ACHAZB_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8615365.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRDGIAIGNPTNDLTVTENYLIVSPLLALAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            476..679
                     /locus_tag="ACHAZB_002758"
     CDS             476..679
                     /locus_tag="ACHAZB_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8615366.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            890..1474
                     /locus_tag="ACHAZB_002759"
     CDS             890..1474
                     /locus_tag="ACHAZB_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8615367.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT          566 a          227 c          297 g          517 t
ORIGIN      
        1 ttaagaaaac gttttcttag ttttgatttc gaggtgatag acaatgaaaa ataagtttat
       61 tttatcttta ggtattttaa ctattggaat tagtgtaact ggaactacaa ttcctacatt
      121 tgctgatagt acaaatgaac caactactgt agtagataat aaccaagaat taactccttt
      181 agatagggac ggaatagcca ttggtaaccc aactaacgac ttaactgtta ctgaaaatta
      241 tttaatagtt tctccattac ttgctctagc agcttctaaa acgatacgag tgacaggaac
      301 gatgattttt gataaagttg gttggccttc tagacctcct caatcacgga attggaaaga
      361 aaaaagaaat ggttattggt actatggaaa attgtattta caaagttata aatcagctgg
      421 aaacaattgg attgcgactt attcaggaac cttaactaga aagtagggat tttttatgcc
      481 agaatttgtg aacacaccaa atttttatca agatagcgat attctaagta aaattaattt
      541 accgaaagaa gaatcagctt tacaagttca gcgaacttta caggagacac aaaaaattca
      601 aaaatcaatt tctaatctag aagattctga agaaaaaaga aaaaaaagag accaaaaaac
      661 atatcaaaaa gaaaaataat gattttaaaa aaagagtgtc tagtcgacat tctttttttc
      721 taattgaaag tgttcataaa atcacccatt tctggacact ttcttttttt gctttatttt
      781 tcaaaaaaac aattcatttc atgttcataa acccataaca atatctatgt tcagttaaac
      841 tatatggata aagttatgtt acaataaaat taatgaagga gtgatttttt tgaaaatagg
      901 atacgcccgg gtatctactg gtttacaaaa tttggattta caagaagaca gcctaaataa
      961 atttggttgt gaaaaaattt ttactgatca tatgagtggg gcaaaaagta atcgaccagg
     1021 attagaaatg gccattgatt ttgttcgctc tggcgataca cttgtcgttt ggcgcttaga
     1081 tcgtttaggt agaaatatgg aagatttgat ttcaatcgtc aatcgactga atgagcgtgg
     1141 tgtgagtttt catagtattc aagaaaatat tacgatggac aaatcaagtt ctacaggaca
     1201 gctcatgttt catttgtttg cggcatttgc agaatttgag cgtaatttga tcctggaacg
     1261 atccgcagca ggacgagcag ctgctcgtgc aagaggacgg tttggtggtc gtcctgaaaa
     1321 attgtctgaa caagatttgg aattactgaa gactttagtg gatagcggta cacctattaa
     1381 gactattgcg gaacggtgga acgtctcaag aaccaccatt tatcgttatt tggcaaaaat
     1441 ggaggagaaa aatggaaaga caaattctca ttagtttttg tcatttaatg ctgaaaattt
     1501 ttgccattta tctagaaaag tgacaatagt atgagaattt tttgtaagtg tctactgata
     1561 acttattctc aaatgttgta aggattttat tcatttgtta atattaa
//
LOCUS       DAYYCY010000267         1583 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.274, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000267 DAYYCY010000000
VERSION     DAYYCY010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.274"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..120
                     /locus_tag="ACHAZB_002760"
     CDS             <1..120
                     /locus_tag="ACHAZB_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFM8615368.1"
                     /translation="YLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(444..527)
                     /locus_tag="ACHAZB_002761"
     tRNA            complement(444..527)
                     /locus_tag="ACHAZB_002761"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(491..493),aa:Leu,seq:cag)
     gene            1055..1555
                     /locus_tag="ACHAZB_002762"
     CDS             1055..1555
                     /locus_tag="ACHAZB_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8615369.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
BASE COUNT          506 a          272 c          265 g          540 t
ORIGIN      
        1 tacttaaatg gtgactactc tgctgcaaac caagaaagag tagcagaaca atatgttact
       61 tcacgttatg gttcttggga agcggcgaaa gcattctggg aagcaaacgg ttggtactaa
      121 gattcattat ttgaacgttt aaacacattc aaaatatagt aagaggtcat gcgatttatt
      181 ctttcgcatg acctcttttt gagtttaagt ttagtcttta ttccttaact atcgtttgcc
      241 agtatttctt gtatcagaat ctataaatct actatttgaa tcaatctatt aggttttaaa
      301 ctgtcccacc ttcacacaaa ttttttttga tttatttgaa tctggttccg ttgtttaaaa
      361 tttttcctat attcttttag ataatatgtt attttctttc tcatcgctcc aggtgtacct
      421 tctctttata aaaaaacaaa ttatgcggct aagaagactc gaacttccac gaggtcaccc
      481 tcactagcgc ctgaagctag cgcgtctgcc aattccgcca tagccgcatc acatctttta
      541 gtttaggatt acatgtgttt tttgtcaaga acatattttt gttctttttc atttctgtac
      601 gccctccctt atgccatcta cacaaataat tcatccgagc aactccatca tctattgagg
      661 gttggtgaaa aaatctgttt tatccatatg atcgatttca atgttaaacg aacgactctg
      721 ggattagtaa ctattttatt tttttcttca tttctttttt cctctttttc tcgattcagc
      781 tatcttcttt catttggata ttgattattt cttctgttga ttactatcct tctggaattc
      841 tagacgctga agtaattcac gttttctttc ttctagagat cataagtaac gaatgaacga
      901 acttcaaata atttttttat tgtaattcat atttgtattg ttcgatgatt cttaatacga
      961 tatgacacaa taaataaaaa taggcatggc tatttacaac aatagtgcta tagtagaaaa
     1021 caaatgaata aggttactta aagaggtgaa tacgatgaaa ctgatcaaac tatgggaagc
     1081 agatttaaag aaagcttatc agctacaaac tagctttgga ccaaatgaga gtggatttat
     1141 caacaatgct tatggctata catttgctga atttttagat tatgtaaaaa aatgcgaaaa
     1201 aacgtcttta ggaatcgatc ttcctgagag ttacgttccc gcaaccgtct ttattttaga
     1261 aagtgatgaa catgagtatg taggaatttt taatttgcgc cataaactga acgaaacatt
     1321 aagaaacggc gctggccata ttggttacgg catctcccct aattatcgta aaaaaggata
     1381 tgccacacaa ggattagcat tagtactaca agaggcaaaa aaaatcggca ttgatgaggc
     1441 acttttgtct gtaaataaaa aaaatcttgg cagtcttaaa gcacaacaaa aaaatggggc
     1501 tgcgatttat cgtgaagatg agaaagaata ctatacgaaa atcaaactga tgtaaaaaag
     1561 aaggattcca aaaggaaagg gat
//
LOCUS       DAYYCY010000268         1551 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.275, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000268 DAYYCY010000000
VERSION     DAYYCY010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.275"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            119..559
                     /locus_tag="ACHAZB_002763"
     CDS             119..559
                     /locus_tag="ACHAZB_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM8615370.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            569..1531
                     /locus_tag="ACHAZB_002764"
     CDS             569..1531
                     /locus_tag="ACHAZB_002764"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFM8615371.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
BASE COUNT          539 a          257 c          339 g          416 t
ORIGIN      
        1 ttaaaaaaag ctcatatagt aaaaaaatga tcttttaagg ttgcattttg aaaaaagtta
       61 agatatagtt tgaacatcaa aagatttgat aatcaaacta attagtggac ggtggaacat
      121 ggaaccagat ttaaaaacag taaatgatta tcttgttagt gtcttcaatg atattttgac
      181 aatcgaagag tctgagctga aaaaatctca attcaaagat ctgtcaatca cagagatgca
      241 cacgatcgag gcaatcggga tgtacaagaa aaaaacaact tcagaagtag ccaaagaact
      301 atcaattact gttgggacct tgaccactgc tattaacaag ttagtaaaaa aaggctatgt
      361 cgaacgtatc cgcagtgaag atgaccgccg cgttgtaaag ttgggactaa cgaaaaaagg
      421 aaagttacta tttcgggtcc atcagcattt tcatcgtgaa atggtcaaga acatcttaga
      481 cggaatggct acggaggaac agcatgcctt gctggatgca ctgaaaaact tgcacgactt
      541 cttgcaagac tataagtgag gatatttaat ggaacaattt ggaatgatta ctgcaacagc
      601 cagcgtcctt cctgaaaaga ttattactaa taatgatctt tccaagatga tggatacctc
      661 tgatgaatgg atttcgtcaa ggacgggtat tcgtgaacga agatgtgtaa caaatcagga
      721 gacttctgat ttatgcattt tagtggcgga aaaattgata aaacagaaaa atctaaaccc
      781 taaagatttg gattttattt tagtagcaac aatgtcgccc gattacacga caccatctgt
      841 agcagcacaa gtacaaggga aattgggagc aactcatgct atcgcttttg atatcagtgc
      901 tgcgtgctca ggatttgttt atgcgttaag catggctgaa aaaatgatcc gctgcggctc
      961 aactaaggga ctggtgattg gtggtgaaac actatctaag ctaatcgatt ggtctgatcg
     1021 tacgactgct gtgttgtttg gcgatggagc aggtgggatt ctcttagaag cgaatccaca
     1081 gaaaaaattg ctgaaagaaa aattagcagc tgacggtacc agaagtagct cattgaccgc
     1141 tggctaccag caaaataaaa atccgttcta ttcagaagat tcagaaggtt cgtattattt
     1201 gcaaatgact ggcagagata tttttgattt tgccgtacga gacgttgcaa acaatatcca
     1261 agaagtcatg aaagacaaac cagttgatta tctgcttttg catcaggcaa acctcagaat
     1321 tattgagaaa attgcccgaa aagtaaagat gccgcaagaa aagttcctga ccaatatgga
     1381 taaatatggg aatacatctg ctgcaagcat ccctatctta ttggatgaag cagtttctag
     1441 tggaaagatc accttaggaa aacagcagaa agtaatattt acaggctatg gcggaggttt
     1501 aacatgggga agtattctca tggagttgta gactgttaaa ttataaaaat a
//
LOCUS       DAYYCY010000269         1544 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.276, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000269 DAYYCY010000000
VERSION     DAYYCY010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.276"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..343)
                     /locus_tag="ACHAZB_002765"
     CDS             complement(<1..343)
                     /locus_tag="ACHAZB_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM8615372.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALECFEQYKENKRRCGRTEIIFPAEEKEYIAKRSTEGWTPDVIIGRAERTFS
                     CSVSTLYRRFKT"
     gene            complement(639..1511)
                     /gene="scrK"
                     /locus_tag="ACHAZB_002766"
     CDS             complement(639..1511)
                     /gene="scrK"
                     /locus_tag="ACHAZB_002766"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFM8615373.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT          413 a          355 c          305 g          471 t
ORIGIN      
        1 ccgtcttaaa ccggcgatag agggtactta ctgaacaaga gaaggttcgc tctgcacggc
       61 caataatgac gtctggggtc caaccttcag ttgatctttt tgcaatgtat tctttttcct
      121 cagcaggaaa aatgatttcg gttctcccac aacgtcgctt attttctttg tattgttcaa
      181 agcattcaag tgctgttcca ccacatttga gaaagttata gacattgtaa attgtttggc
      241 gtccacgttt aagctgcttc gcaacgatag caaccggagt ttcttgatga aaatatgctt
      301 ctatcattac aagctcgttt ggtgtaagat gggtataagt catttatgtt cactctcctt
      361 gtatgcttta gcgggtatta cgatttgagt gtaacataaa tggctttttt atttgtctcg
      421 cttaattata caaacggcgt gtaaaaaact attgcacaat acaagtggcc tacggctatc
      481 gaaatttcca taactttcga gcaagaattt atctcattca aggcttgatc ttccaagtaa
      541 aacaaaaacc agttaagcac tctgcctaac tggttatgac taaattttca gttgtatctt
      601 tctctaccaa cactaaatgt cgaagagcct tttttcgttt atgaatgtaa gaccgcttct
      661 cgagccaatg ccaaacaacc gagcgttcca gcgttgtctt ctaatgctgg ggtcacgata
      721 tatttgctta actcaggtgt ttcgacatac ccattgacca attttgcaaa tgcgtcgtgg
      781 acgttttgca gcatatggcg ttgtttcatc acaccaccgc caaaaatgat cacatctgga
      841 gaaaacatca aggttgtatt gtaggcacat tgagcaatat aggaggcttc gatttcccaa
      901 taaggatcat cttctgcaac ctcttgtcct tttttcccta agcgtttttc gatggcgggt
      961 ccagcggcca tcccttctaa acagttgcca tgaaatggac agctgccagc aaagtcatct
     1021 tttggatgag ggacgacgag catatgcccc atttctggat gactaaaccc ttcgataaag
     1081 cgcccttctt gtaaggcacc gccgccaaca cccgtaccga tcgtatagta aacgacactg
     1141 ctcaatcctt tgccactgcc gaagacatac tcaccatagg cagccgcatt gacatctgtt
     1201 gtccaagaaa tcgggatcgg aaattctttt ttcatggtac cgatgaaatc aaaattttgc
     1261 catgccaatt tcggggtcga ggtaatgtac ccataagtgg cggaatcgcg atggatatcg
     1321 ataggcccaa atgagccaat cccaatccct gctaaatctg cttggtattg tttgaagaag
     1381 tcaatgacta aaggcattgt ttcctcaggt gtcgttgtcg gaaagctcac tctttccttg
     1441 atcgtcattt cttcatcgcc aatggcacac acaaattttg tgccgcctgc ttcaattgca
     1501 ccgtatagca tagacatttc tccctttatt ttagaatgaa atca
//
LOCUS       DAYYCY010000270         1502 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.277, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000270 DAYYCY010000000
VERSION     DAYYCY010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.277"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1458
                     /locus_tag="ACHAZB_002767"
     CDS             <1..1458
                     /locus_tag="ACHAZB_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HFM8615374.1"
                     /translation="QQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDTVAN
                     GGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIVLAL
                     PFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGALLA
                     PLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEERKGF
                     IRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVTAEE
                     KPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNPIFI
                     FILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPLWLL
                     GSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFYTPG
                     TESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          408 a          289 c          317 g          488 t
ORIGIN      
        1 cagcaagaca agggcttctt cgggcagccg aagggattgt caactttgtt cttcactgag
       61 atgtgggaac gatttagtta ttacggtatg cgtgcattac ttatctatta tatgtacgat
      121 acagttgcca acgggggact tggattacca cgagcaacag cattagctat catgtcgatc
      181 tacggatctt tgatctacat gtctagtatc gtcggcggat gggtatctga ccgagtgtta
      241 ggagcaaaac gcactgtatt cttcggtgga gttttcatta tgcttggcca catcgtcttg
      301 gctttaccat ttggtgtaag tacattgttt gtttcgatgg cattgattat tatcggaacc
      361 gcctttttga aaccaaatgt ttctggcatg gtcggacatc tttattcaaa gactgacttg
      421 cgaagagatg caggattttc gattttttac atgggtgtca acttaggggc attattagca
      481 ccactaatcg ttggtacaat cggtcaagaa tataactatc atttagggtt ctctttagct
      541 gctattggga tgttctttgg attattacaa tacatcatcc aaggacgcaa gacattagac
      601 ggagtgggta caacaccgcc gaatcctttg acaactgaag aacgtaaagg attcatccgt
      661 aacttgatca tcgcacttgt tgttatcgct gcagtattcg gcggggcaca actaactggt
      721 catctgacaa ttgacttttt tgtcaataca atcagtgtat tggggattat cttaccgttg
      781 tattacttta tcaaaatgct gacttcaaaa gatgtaactg ctgaagaaaa accaagagta
      841 tgggcttata tcccattatt ccttgcagca atcattttct ggtcattaca agaacaaggt
      901 tcatctatct tagctttatt tgctagtgaa cgtacacaat ctacgctatt tggtgtaacg
      961 atccaagcaa gctggtatca atcattgaat ccaattttca tctttattct gacaccagta
     1021 ttcgtaacga tgtggacgaa acttggtgaa cgtcaaccat ctacagtagt gaaattctct
     1081 cttggacttt tatttgcagg attatcgtac ttgctattga tgttgccggg tatgctttat
     1141 ggtacaagtg gaaaagtaag tccattgtgg ttgttgggaa gtttcttcat cgtggttatt
     1201 gctgaattgt gtctttcacc agttggtttg tctgtgacaa caaaattagc accacgagca
     1261 tttgaatcac aaacaatcgc tatttggttc ttagctgatg cttcttcaca agcaatcaat
     1321 gcacaaattg cacgcttcta cacaccggga acagaatctg cttacttcgg tattatcggt
     1381 gcagtagcaa tcgtcggtgg tatcttgcta tttactgtga aagaaccaat caaaaaacta
     1441 atgggctcta ttcattaatc aatgaacata aaaacaggaa ttgcttattt gcagcagttc
     1501 ct
//
LOCUS       DAYYCY010000271         1489 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.278, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000271 DAYYCY010000000
VERSION     DAYYCY010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1489
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.278"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            123..>1489
                     /locus_tag="ACHAZB_002768"
     CDS             123..>1489
                     /locus_tag="ACHAZB_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8615375.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEK"
BASE COUNT          537 a          204 c          273 g          475 t
ORIGIN      
        1 aaaaatgaga ataactgatg aaaaaacaag cataatccat cataaaatca gtataggtga
       61 aaagatgaac tttcatctgc atatcaagta attggtatga ggcatttttt gaggtgtagg
      121 atatgaagtt ggattacctt atagaagagg aagatagaga aaaactgctg ctagctagat
      181 atttagaatt tgagaaaaag aattatgtag tgctgcctaa gttatatgaa acattaggtt
      241 tgtctaaatt taaaacaaga aattatatca tagaattaaa taaagaattt actgaattca
      301 aaacaaagcc agagatccaa atttcagaag aagcagagat ccatgtcgaa cgtctgaatt
      361 tgacggatat caaaaattta agaagaatct actttcaacg atcaaaaatt gctcgactgt
      421 taacggagat cgtggattca aaagtaacga tcgaaaaatt tgcagagaat cattttttaa
      481 gcatatcaag agcctatgta aaaagaagag aattagtctc ttttcttgaa cagcatcaaa
      541 taaagttaaa gaaaaacata ttagttgggg aagaaataaa catacggaac gtattatttg
      601 gggtatattt cggggtcttt aatggttttt ctatgccatt tagagaaagc gtcatgaatg
      661 aagcaaagag tatcgtgcaa tattttgaat atctgttcaa tttaaaactg tctgcaacaa
      721 aacgaatcaa attcaatttg ctaaccacta ttagtttgtt gagggcctat gggaatcaac
      781 caatcggaaa acgtttcttt tcagatgaat actttaagag ctcagaagga atgaagatca
      841 tcaaggattt atcacatatt tcttttcttc atatccctaa agagcagatc attgatgaga
      901 tttcttatat catgttgttt ttgatcacgg aagaagaatt tgaattagat ggcgcatttg
      961 gacttgcgga agctcatttt catgatatcc acatgattag tcaaaaaatg tcagtggaat
     1021 tgattgatca attaccattt tcgattgaaa tctctgaaga agaaagacta tatatacgaa
     1081 aagaacttcg tatgggtatg atgcatgtca atcgaaaaaa ttggatattc gactttgaga
     1141 tgactagctt tacgtctaaa aagcagctac agtttttctt cgagacgtat ccaatattca
     1201 gtacagcaat ccaaaaggtc atacaagaga ataaacgcta tttcttggga aaagatgaac
     1261 gtttcatgat tcgcttattt tatgattatc tctttttatt ggtcggtatt tgtccggtat
     1321 catattttga agcaccgatt catgtctgta ttgatttttc gcaaggatct gcttataccg
     1381 attacattat ttctcaagtg aaaggattta aaaattacaa tattgtgatt gagagtcgaa
     1441 tcacttctcg tacgaatatt tatttatctg attgtttact tgaaaaatt
//
LOCUS       DAYYCY010000272         1489 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.279, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000272 DAYYCY010000000
VERSION     DAYYCY010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1489
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.279"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            759..1145
                     /locus_tag="ACHAZB_002769"
     CDS             759..1145
                     /locus_tag="ACHAZB_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFM8615376.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            1239..1430
                     /locus_tag="ACHAZB_002770"
     CDS             1239..1430
                     /locus_tag="ACHAZB_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8615377.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
BASE COUNT          553 a          231 c          208 g          497 t
ORIGIN      
        1 accacgatag gtaactttaa acaaacatga agagacttta ataagacttc tttatctgaa
       61 aacacttttg tttctctctt tcctttctac atcattaaat agtattttat acgttatcta
      121 aaatactatt cctctgtcaa aataagctaa cttacttcat tataaaatat ccacttatcg
      181 ctttcatgta tattccaccg taaaaagtac attttatcac cgagaactca tcatttttca
      241 tcatgatgaa tactatttac atatatctag ttcaaagaca ataattatta tgatccgtta
      301 ttatctaagc actatacaga taataggaat ttttcgtttc taaatattcc aatttgggaa
      361 tccaataaaa attttcgttc aaatacgtgt ctattcgaac tcttttctag ttgttaatta
      421 tgaaggggga agaaataaaa aatacaatct atacacgaat aagaatatta tccaaaagga
      481 ggttttagaa aagatttatt atacgtaaaa agctcaagac attttcttag ccataaggac
      541 atgtttgata tttaagaaga tatatgaatt ttcttttcta agtataaaga taactatcca
      601 ttatcaacta aaaaaacatt tttgaaaata aacatcagca gtcctatcaa tgcatgtgag
      661 cttatagaag ttattcgcag cgcagcataa ttgcattagc ttcttttcgt gttttttgcc
      721 tcttttccct ttaaatctta atggaggagg tgtgcaatat gtcagataac gacgaacgcg
      781 taaatgccca gttgattcga ttctttgaaa aggtcatttt gaacaccgct aatacttatt
      841 ataaaaaaca agaagaaatt caaaaacatg aacaacttga tcaactccca ccatatttgt
      901 ttactaaaga tactataaaa tttaaacaac cgattaatgt tttaaatgtg actctccttg
      961 ttgaagatat aaaattagca aaaatccttc ctttgttgaa agaacctgaa caaatttttc
     1021 ttatcgaaaa atttatcttt gataaaactg ataaagaaat tggaaaggtt ctggggatta
     1081 caagacaggg tgcgacaaat ttaaaacatc gactttacaa aaaaattcaa aaaatgctgg
     1141 tttaatatct gcataataac taattttgta tgcttctttg ctcgttctcc tccttcccaa
     1201 agaaatagat tataagtcca tgggaagaag gtagatttat gaattcttta aaagaagatt
     1261 tcattttagc aaaggctgga aatgaagaag ctgttgaggc aattctcaaa agatttagct
     1321 ctttgattca taagcaatcc tggagaactg gaaaatatga tcaagattgt tatcaagaat
     1381 gtatgcttgc aatatatcta gctatctcga aattcgaaat taaagaataa ctcaactaag
     1441 ggagtctgtg acataagtaa aaagatagtt tcaaatttcg aacaataaa
//
LOCUS       DAYYCY010000273         1475 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.280, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000273 DAYYCY010000000
VERSION     DAYYCY010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1475
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.280"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            125..1294
                     /locus_tag="ACHAZB_002771"
     CDS             125..1294
                     /locus_tag="ACHAZB_002771"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFM8615378.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
BASE COUNT          537 a          262 c          236 g          440 t
ORIGIN      
        1 aatattctat caaaaatgta agtagtaaat ccattactat aagattaaaa taaatgttat
       61 caagatacaa gctattttag ctttattctt tctgtaaaaa agaaaggaga ctaaataaac
      121 tattatgaaa aaaggaaaac atgctaaaaa aagaaaatat tcatttttaa tgatcgttgt
      181 cgtgttgttt agccttagtg cgttctctac aggaactatt cttggatggc aattttacaa
      241 taatgaacac tctcagatag ttgcccatgc tcaatcagct caaaaaaagt tgatactgac
      301 taccaatgaa caacagctcg ttgaaaaaat cgatcaacta ttaagaaaaa atgattatat
      361 tggctcaatt tacgtaaaga aaaataatcg catcatcatg gaaaaaggct atggctatgc
      421 gaataaactc acgcaacaac caaatgatcc ttctctttat tatcagattg gttcggttca
      481 aaaagcattg actactcttt tgattttaaa acaagtcgag cgtgggcacc tttctttaga
      541 tactaagctg gctcaattct atccacaaat tgctggcagc cagcaaatta ctatccgtga
      601 cttgctgtat atgcgctcag gcttgcaccg aactgcttct ccaaccatac cgatgactga
      661 tgaagaagtc gttcaatttg cgattcagca cttaaaattt acagattatc agacctctca
      721 ttatgattca ttgaacttta ccatattgac tggaattttg atcaaattaa caaatcaacc
      781 gtatgaaaaa ttattgaccg atgatatcat cgaaccttta ggattacagc atacaaaatt
      841 ttatgaccaa gtcaaaaatt catccaatca tgcactatcc tatcaaatgt ctaaagagga
      901 tgattacttt aaaccactga cagaatctga aacagagatc cataacgaat tagggactgg
      961 taatatcagt atgtccgtct atgatttaaa tacgctgttt acacaagttt tatctggaaa
     1021 actagtttcc aaagacttac tcttctcatt atggcgtgaa aatgcgaatg gacatccata
     1081 tagtggtggg gtatatagcg gagataatta tatcctgtcg caaggaaata tcgactcttt
     1141 tgatacatct gctatattta gcaaagattc acaggatgct atcatcatgg aaagcaatat
     1201 aaaagctgac aaaactatca aactgcctac tgctgatcta agaaaccaaa tctatgacct
     1261 gatggaagga actactaagt tggcaaatag ctgatatgat gaaaagatag caaatactac
     1321 ttctttgaat agcatttgct taaaaaaagt actcgactaa acgtaggaaa gagtgtggga
     1381 caaatctaaa aatcaatttt tgctacagac tttaattcca aataaacaga acagaaagag
     1441 gtgttcttct ttctgttctg tccaaattat tctaa
//
LOCUS       DAYYCY010000274         1454 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.281, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000274 DAYYCY010000000
VERSION     DAYYCY010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1454
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.281"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..625)
                     /locus_tag="ACHAZB_002772"
     CDS             complement(<1..625)
                     /locus_tag="ACHAZB_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HFM8615379.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFD"
     gene            complement(784..1050)
                     /locus_tag="ACHAZB_002773"
     CDS             complement(784..1050)
                     /locus_tag="ACHAZB_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8615380.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(1040..1396)
                     /locus_tag="ACHAZB_002774"
     CDS             complement(1040..1396)
                     /locus_tag="ACHAZB_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFM8615381.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT          406 a          279 c          181 g          588 t
ORIGIN      
        1 gatcaaaaat atctaagttg tcatcaaaat ctttatcagt tccggtaact tttttgttaa
       61 ttactgaagg tgtcttgtaa gaaatagaat ctaaatcaaa gcgttttgat tctaaattat
      121 tttgtgcttt ctcaattaaa taattcactt tttttgtgag aaaccttctg atattgtctt
      181 tgctatatgt tccataaaaa cccttatctt ctaaagagtc cctgtacttc ttaacttcta
      241 tatactcttc agccaaaact tgtggaactg gttcatctaa gaaaaaaacc cctacctcgc
      301 ctttgctatt tttataaaag taatcaattt ctgctatagt tcccgatcta taattatcaa
      361 ctggagaacc caacttatgg ccaaaaatcg ctaataatat atcactattt tctacaattt
      421 gtttattaat aacactttgc ccagagctat tatttatata agtaggaatc gcattcgttt
      481 cccatcgaat tggcatcaaa attacttttg tattatctgt attttctata ttccatctat
      541 atatggtttc ttctacttca ttcctctttg ctgctatatc acctggtgat gctataaata
      601 cttttattag atttatggtt ttcatttatt gtacctttct ctttatgtct ctttaaagta
      661 atttttgtat aaaatttatt gacttttaca ctataggaca acttttatct atttagtaca
      721 aaaaggccca ctaaactaat tgattagtta ggagcctatt acaggtatat tttcaactcg
      781 cttttaagca aattctacta ccagcttttt gcctaatgcc ctagcgatat catcaagttt
      841 atcgaaagat acgttatccc ctctctcaat tcgagcaata gtagattgag ttgtatgtgc
      901 tttttgtgca agctccgctt gagttaggcc tgcttcctcc cgtgcatgat aaagagcaac
      961 cgcagtttcg agttttcttc cttcacggtc atatgcttct gcaaaatctt tgtctttttg
     1021 cttttcttca atcaattttt taatcatcat ttgtgaacca ctcctctcta agttcttttg
     1081 catgtttaag ttcacttatt ggtgttttct ccgttttttt agtaaatcca tgggtaatga
     1141 cataccttcc attgaccaca tggaaataga tagctctttg gatgtttgat cctaccttac
     1201 ttcttaattc gtacaaatta gtgtctaatt ttttcaccca tttcaaccgt tgagctacta
     1261 gcatcccatt cttttctgtt tcttctattg tacgtaaaag cttcgcacta tctttttttg
     1321 gtaatgtttc caaccattct aaaaattcat catgaccatt aggtctttta aatgtttcaa
     1381 aagatatttt ttccataccc aattatatca tatattgatt ttatattata gtatataaca
     1441 taaaaattcg aatt
//
LOCUS       DAYYCY010000275         1440 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.282, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000275 DAYYCY010000000
VERSION     DAYYCY010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1440
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.282"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            105..1406
                     /locus_tag="ACHAZB_002775"
     CDS             105..1406
                     /locus_tag="ACHAZB_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFM8615382.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT          474 a          288 c          264 g          414 t
ORIGIN      
        1 gatagaaaat cattctatcg gtcccttttg agcatgaaaa aacaaaacat acagaagctc
       61 cgttagaatt gaatcaccac aacacaatca aaggagcgtt ctgtatgcct gatcttcatc
      121 atatcaaact actacttgga atcaaagata aaaacatttt catcacaaat gtggaatcga
      181 agagcattaa gaatatcaag agtcttgttg tttctgcgac gttaagtaaa gaaattcgtc
      241 gctgtccttt gtgtaaacag atgaatcatg aaggcatgat cgttaagaac ggaaagaaaa
      301 aatcgctcat tcaacttaat aaatgtgcga accaactgac ttatcttgct ttagcgaaac
      361 aacgctacca ctgtcgtggc tgccacacct attttacggc gaatacctac attgtcgata
      421 gaaactgctt tatcgctaaa caagtgcgct ataaaatttt agaagagcta actgaaaaac
      481 aagcaatgac gacaattgct aagcattgcg gcgtttcttg gtcgaccgtt tctcggacac
      541 tcgcttctct gatccccatg acaaaagtaa aaagaaattg gttaccgcgt tgtttattaa
      601 tggatgaatt tcgttcactg aaaaatcaag tcggtcccta ctccttttct tgtatggatg
      661 gcgatactgg aaaattactg gatattcttc cttcacggaa gaaaaaggct ctcgtttctt
      721 attttatgca atttgaacga cgcgcacgat tgaaggtaaa ggtcatcgtg acggacatga
      781 atgtttctta tgcctctctg ataaaggaat gttttccaaa ggcaaaactc gttgtggatc
      841 gttttcacat tgtcaaacat ctcattcgta aatttgagga tattcgcgta cgaatcatga
      901 aaagctttga tcgaaacgat ccaacacaag ccaaatatta tcgacaatta aaggcgttgt
      961 ctcggcttct gatcaaacga caggacaagc ttgtttatga caaatggacc aaatggcgca
     1021 attttggttg ggcgtatctc accgaaagtg aagtggtgga tcgtctgctt tcgatctctg
     1081 acgaattacg aatcgcctat ttttactatc aagaaattct tcaagcgttt cacgacaaag
     1141 aggcggacac tttctttaaa cttgtaagaa cgatgcccaa ttccgttcca aaagaactgc
     1201 attctataaa aaaagctttt ataaactatg aatccggcat tcgtttagcc ctcgaattgc
     1261 cttattcaaa tgccaaaata gaaaatctgc atacccacat caaagcattg aaacgagtcg
     1321 cttatggctt tcgaagcttt cggaaaatga agaccagaat tttcttacta aataatctga
     1381 ttacctatga atcaaaaaac atctaaagta gaaaaggaat tctaccttag atgttcttat
//
LOCUS       DAYYCY010000276         1436 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.283, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000276 DAYYCY010000000
VERSION     DAYYCY010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1436
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.283"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            304..1344
                     /locus_tag="ACHAZB_002776"
     CDS             304..1344
                     /locus_tag="ACHAZB_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFM8615383.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
BASE COUNT          567 a          193 c          224 g          452 t
ORIGIN      
        1 atgcataagg tagttcttaa tttatttttt gttttgacac tggtagtact taattatctt
       61 taattttaaa actaaatttt attcatacgt ttttctggta gtatttaatt ataaataata
      121 tttatttaaa gtagttctta atgactttaa aggtagtaca taattcattt tatttctgat
      181 tttaggtagt atttaattaa aaaacagttt taatgcgttt gcatttttta tttatctttg
      241 ctattttcga aaagagtagg taattttaca taaataaaac aacgtgaaag tagaggatct
      301 acaatgactg attttaaatt ttttaaggcg gatagagttt acaacgaatt attttatcaa
      361 tttccaaaag tctttattgt ttctgacgaa tacaaaaaaa tgaaagattc aactaagatt
      421 gcctatatgc ttttaaaagc aagattagag atcgcaatca gcaaacggca aatcgatgaa
      481 gaaggtaatg tttattttac ttatacgaca aatgaactat gtagagtatt aaactgccaa
      541 aaacaaaaag cgatagcaat caaaaaagag ttggaatcct ttggtttatt attacaaaag
      601 cagatgggat ttaacaaaca gttagggaaa aataatccta atagactata tctagcagaa
      661 ttaaaagtct cagaaaatga tatctactta ctcgaaaaat ttgatagaga gaatagggaa
      721 aacgttgata aatcagaggg tatgaaaatc atacccaccc tcgacgaaaa atcagacgct
      781 gaatcccttg gggctcaaga gggtatgaaa atcataccgt gccaaaacgt tgataaatca
      841 gagggtatga aaatcatacc agaacttaat aataatatat tagacactaa tagacacaat
      901 atagacactg aaaaagaccg cctacaagat caattgttgt tagacaattt tgagacaatt
      961 atgacaaacg acagcattgc tacgtttgtc cctgaacgag tattaaattt gataaaaaca
     1021 ttttcttcaa gttacagtga agctcaaaaa accgtccaga ctattcataa tgcaaagaaa
     1081 aaagctgaaa tagaaagtga tatttcgata gtttttgaag aactcgatag ttattatgtc
     1141 aatgcagaac aagaattata cacgacactg ttaaaagcct atcaaaaatt aaaaaccgaa
     1201 aaagtcgaaa atatccagaa cctgattttt gtctatgtaa aaaattggtt tatcgaaaaa
     1261 ccaatagctg ctaaagtatc aagtgaaaaa cgtttgaatt atgcaagctc cccaagcact
     1321 attacgaaag actggttaga gtgaaacttt actgacaaag tgattgttgc ttcatggagt
     1381 aacattaaaa ttaaaaattt tttattattc taattatatt acgaatatta caaaat
//
LOCUS       DAYYCY010000277         1424 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.284, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000277 DAYYCY010000000
VERSION     DAYYCY010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.284"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..276)
                     /locus_tag="ACHAZB_002777"
     CDS             complement(<1..276)
                     /locus_tag="ACHAZB_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
                     /protein_id="HFM8615384.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAI"
     gene            401..1186
                     /locus_tag="ACHAZB_002778"
     CDS             401..1186
                     /locus_tag="ACHAZB_002778"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SinI family restriction endonuclease"
                     /protein_id="HFM8615385.1"
                     /translation="MPNKNRTFESILNEGLTTEPASIIFDEVIDDVEDSKYHKNLKEL
                     FYFALNNGPSLFTGISVPDSDNPLIMAKIYLTKWCNKYIRDRNNPALKKPLKTFGEKD
                     AALTTRVAANANIDDQKVLNDYLRGHFLYMSAENMNGSILEEYLAEVLEPEGWIWCAG
                     SVYRAVDFCYLGTSPILLQVKNKYNTESSSSSAIRVGTTIRKWNRLNKSTKISGLDSP
                     IPNWKALIEMTEASKELAAKLTENSYLAYINEKSTRELWTLDD"
     gene            1179..1421
                     /locus_tag="ACHAZB_002779"
     CDS             1179..1421
                     /locus_tag="ACHAZB_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8615386.1"
                     /translation="MTNNILISIYPRYMEKIINKQKNFEFRNFYLTSDPYNIFWIYET
                     IPTKALKYKLIVKNPITKLKKNQHYFLGDDEFEQLV"
BASE COUNT          534 a          231 c          216 g          443 t
ORIGIN      
        1 aattgcatat cccaaaatat caacgattgt ggcaatcaga tcttcttttg attcttcatc
       61 tgaaagtaat tcacgataca acattgtttg tttgcttact gtgtcatatt cctctaagga
      121 ttcatctgct aaaagaacca cttgttccaa caaacggtcg gataaatatt tatagaaaat
      181 cagtcccaat aaataatttt tatattctga cgcatccatt ttactacgga gattatctgc
      241 cgcactaaat aatttttgat ttaattctgc acccaaaaag atacactcct atctcttatt
      301 taaagttgct atataccctc ttattttagc atactccctt ttagaaaaag caaaaaaaaa
      361 agaaaaaatc ttattcttga taacaagcta cgtgacgtat atgcctaaca aaaatagaac
      421 ttttgaatca atcttaaatg aaggtttaac cacagaaccg gcatccatta tttttgatga
      481 agttatcgat gacgttgaag atagtaagta tcataaaaat ttaaaagaac ttttttattt
      541 tgcactcaac aatggaccaa gtttgttcac tggtatatca gtacctgata gtgataatcc
      601 attaattatg gcaaaaattt acttaacaaa atggtgtaat aaatacatca gggataggaa
      661 taacccagca ttaaaaaaac ctcttaaaac atttggcgaa aaagatgctg ctttaactac
      721 tagagtagca gcaaatgcta atattgatga tcaaaaagta ttgaatgact acttgcgtgg
      781 acatttttta tatatgagcg ctgagaacat gaacggaagt attttagagg aatatttagc
      841 cgaagtgtta gaacctgaag gttggatttg gtgcgccggc tctgtatatc gagctgttga
      901 tttctgttat ttaggaacct caccaattct tttacaagtg aaaaataaat acaatacaga
      961 aagcagttcg agttcagcta taagagtagg aacaacaatt agaaaatgga atcgtttaaa
     1021 taagtcgaca aaaatttctg gtttggatag ccctatcccc aattggaaag cattaataga
     1081 aatgactgaa gctagtaagg aactggcagc taagttaact gaaaatagtt atttagctta
     1141 tattaatgaa aaatccacac gagaattatg gacactcgat gactaacaat atccttatta
     1201 gtatctatcc acgatacatg gaaaaaatta taaacaaaca aaaaaacttt gaatttagaa
     1261 atttctatct tacctcagat ccttataata tcttttggat ttacgaaaca atccccacca
     1321 aagctttaaa gtataaattg attgtaaaaa atccaattac taaacttaag aaaaatcaac
     1381 actacttttt aggagacgat gagtttgaac aattagttta aaag
//
LOCUS       DAYYCY010000278         1424 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.285, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000278 DAYYCY010000000
VERSION     DAYYCY010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.285"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(150..1337)
                     /locus_tag="ACHAZB_002780"
     CDS             complement(150..1337)
                     /locus_tag="ACHAZB_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HFM8615387.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          414 a          309 c          249 g          452 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttcact ttta
//
LOCUS       DAYYCY010000279         1398 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.286, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000279 DAYYCY010000000
VERSION     DAYYCY010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.286"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            37..651
                     /locus_tag="ACHAZB_002781"
                     /pseudo
     CDS             37..651
                     /locus_tag="ACHAZB_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(1021..>1398)
                     /locus_tag="ACHAZB_002782"
     CDS             complement(1021..>1398)
                     /locus_tag="ACHAZB_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HFM8615388.1"
                     /translation="PIEFDNDERLLLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRS
                     YLSDRVFDVLEKLSKSGILKEEYDSSFDEYPSRYILISEDYKKFKRYFLKNEKKISVI
                     MEKYKAPIQADPFSNLDESDLPF"
BASE COUNT          504 a          230 c          205 g          459 t
ORIGIN      
        1 tgtattataa acgttataga aaagtaggga ataaatttga aaattgctta tattagagtt
       61 tcatccatcg atcaaaatga acaacgtcaa attgaagaaa tgaaaaagtt tggcgctgaa
      121 aaaattttta tcgaaaaaca atctggagcg acgattagtc atcggccgat tttccaggaa
      181 gctttagatt ttgtcagaga gcaagatatt tttattgtag aagccattga tcgacttgga
      241 cgcaattatg acgagattat cgattcagtg aattatctaa agaagaagaa tgtgcgactt
      301 attatcacca gtttaccgat catggctgaa gcaattggaa atcctctcct agataaattc
      361 atcaaagact taatcattta aatctcagcc atgattgctg aacaagaacg tactgaatcg
      421 aaacgacgcc aggcacaagg aattaaaatc gctaaagcaa acggcgtcta taaaggtcgt
      481 cctaagttat acagtgcaga caccaaagat cctcaacgtc gccttgtcta taggagtatc
      541 gttcaggatc ttgagaatgg ggttgctatc tcaaagattg caacagacta caatgtgact
      601 aggcaaacaa tttaccgaat aaaaaaagaa atcgaccaac taattgtatg atttcttttt
      661 tgaatcacgt aattattaat tagctttgtc atcaatacag acaatcattt tagattcatc
      721 tagctaagtt gattacattt atgttaaaaa gaatttagtt aacataaata taattgatgc
      781 ataaatcgtc cttatttaaa ggcatttaac attgacaact tagtataatg tatcttatgt
      841 aaactaataa gatttgaatt ttattttata cacactcgat aatagatcaa ttttatggtc
      901 ccccgcattt acgattgcac aagagaaaca taaagtcgac ctattagcat ttcttttcaa
      961 ttcaaacatt aaaaaagttt atacctctgc tactaaaaaa tgaaattatc tatttaaaat
     1021 ttaaaacggt aaatctgatt catccaaatt ggaaaatgga tcagcctgta ttggagcttt
     1081 gtatttctcc ataataacag atatcttttt ttcatttttt aaaaaatacc ttttaaattt
     1141 cttatagtcc tctgaaatca aaatatagcg tgatggatac tcatcaaaag aagaatcgta
     1201 ttcttccttt aaaattcccg atttacttaa tttttctagt acatcaaata ctctatcact
     1261 caagtaactc cttaaattgt tatcactttc ccattgaact acttttctat aagttgaatt
     1321 atcagttgaa aagatagaat tttcatccat aaacgcatat attagcagca atctttcatc
     1381 attgtcaaat tctattgg
//
LOCUS       DAYYCY010000280         1394 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.287, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000280 DAYYCY010000000
VERSION     DAYYCY010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1394
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.287"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            33..1372
                     /locus_tag="ACHAZB_002783"
     CDS             join(33..474,474..1372)
                     /locus_tag="ACHAZB_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HFM8615389.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRAENAYFKKVRNLDSRTRF
                     EQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYWVKKLDKPDKYSKIKQEITAIVK
                     ESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKRYKSYKGTVGKIAKN
                     VLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIISYSISTSPTYKLIEE
                     MLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMSRKGNCLDNSVIENF
                     FGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLSPVEYRKQSIK"
BASE COUNT          579 a          181 c          244 g          390 t
ORIGIN      
        1 gactagaaat ctaattaaaa ggggtgcttt ttttgcggaa atatacattt gtttttaaga
       61 aaaaagtagt ttcagactat ttaaataacg aaggcggcta caaatatctt gcacataaat
      121 atcaaataaa tcgtacactg gttagacatt gggtaaggat ttataactat catggttggg
      181 aaggcttggt tggaggtggc aaaagctaca ctacaaaatt taaacttgat gttatagaat
      241 atatggaaac aaatggtctt tctatccaag aaactgctaa aaaatttaat atcggttcaa
      301 atagaactct aagtaaatgg atagagcaat atgaagaagg cggtgcttct tcacttgaga
      361 gccaaaaaag gggcagaaaa attagtatga attccaagct aaacattcct aaaaaactta
      421 aagatgagtc tcttgaagaa gaagttattc gtttaagagc agagaacgca tatttaaaaa
      481 agttagaaac cttgattcaa gaacaagatt tgagcaagaa gaaatcaagg ttaaaacaat
      541 ccttcagctc aaaaaagaat ttaaattgaa tctattactt tcaattgcac aattagcgaa
      601 gtcaacttat tattactggg taaaaaaatt agataagcca gataaatata gcaagataaa
      661 gcaagaaatt acagcgattg taaaagaatc aagaaactct tatggttatc gtagagtcac
      721 tttagcgtta aagatgaaag gatatacaat caatcataaa acagttagaa aattaatgtc
      781 ccaaatggga cttacctgtc aaatccgaat aaagcgatat aaatcttata aaggaacagt
      841 aggaaaaatt gccaagaatg tgttaaaacg aaatttttca gtagatacac ccaataaaaa
      901 atgggtgaca gacgtcactg aattcaagat aaaaggaaga aaaatctatc tatctcctat
      961 acttgattta tttaacggag aaataattag ttatagtata tcaactagtc caacatataa
     1021 actgattgaa gaaatgcttc aacaagcaat taaaaaaaag ggaactgagg gttcactgat
     1081 tctacattca gatcaaggat ggcagtatca gatgccacaa tatcaaaaaa aattaaaaga
     1141 gaataacatt attcaaagca tgtctagaaa aggaaattgc ttagataatt ctgtaataga
     1201 aaattttttt ggtgtgctta aatcagagtt tttttatcga gaaaaattcc gatcaattga
     1261 gatatttcaa agtaaattaa atgagtatat taggtggtat aataacaaaa ggataaaact
     1321 aaagttaaat ggactatctc cagtagaata tcggaaacag tccattaaat agttcaactt
     1381 ttggggttca gttc
//
LOCUS       DAYYCY010000281         1364 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.288, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000281 DAYYCY010000000
VERSION     DAYYCY010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.288"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(140..1288)
                     /gene="tnpB"
                     /locus_tag="ACHAZB_002784"
     CDS             complement(140..1288)
                     /gene="tnpB"
                     /locus_tag="ACHAZB_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element RNA-guided
                     endonuclease TnpB"
                     /protein_id="HFM8615390.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
BASE COUNT          392 a          242 c          217 g          513 t
ORIGIN      
        1 taaacctgac ggttcttaac gcttgaagtg ggagcttctt gggaatagag catacttgat
       61 agcgtatttc tttactaaca gcggtgtctc ttatttacca agctatcccc gtagttccta
      121 cggttcttga tgattggatc taagccaatc ccatagaatg tagtcttaga ccttcggtca
      181 gaatattgat actagcattg atatcacgat catgatgagt atgacaaaca ggacaagtcc
      241 attctcgaat ttcaagagat ttcttgccat ctttatgtcc gcactctgaa caaatctgac
      301 tagacggaaa ccatttatct actttgatga tttttcgtcc ataccagtca gccttgtatt
      361 gtaatttagt cacaaacttt gaccaagaaa catcagaaat acttttagct aatttatgat
      421 ttcgcaacat accttttgtg tttaagtctt caatacagat aatatcgtga tttttgatca
      481 tttctgtact caacttattc agaaaatcag tacgttgatt cattactttt tcatgcaatc
      541 tagctacttt gcgtttttgt ttttgataat ttttagcttc aaatagattg gtacctttat
      601 ttttagccaa taaagcacgc ctagacaatt tgcgttgttc acgctttagt ttcttttcca
      661 ttttggacgt gaatttatta ttatcaattt tttgtccatc agaaagaatc gcaaagtcca
      721 taatacctaa gtcaatacca attgtggaat tagttttagg taattcgata atttcttctt
      781 tacacaacaa agaaatatag tatttaccgc tagaatgacg tgatattgta gcggatttga
      841 taatcccttt tggttgtcta tgaagcttaa ttcttactaa tgacttcaat ttaggaactt
      901 tgatgaattt actatcaatc aaagcaactg taccattttg attattcgtt gtataactct
      961 gaacaggatt tttcttactt ttgaaacgtg gaaatccaac ggatttatcc cgaaaaaaat
     1021 tcttgtatgc tttatctaaa ttaagttggg cattggctaa agcaaggcta tcaatttctt
     1081 tcaaaaacgg aaattctttc ttgtatttag ctggtgtcgg aaaagtcatt tttttagaag
     1141 aatcattttt aacttcttca tacgcctttt ttcggtcatc aagcattaga ttgtagacct
     1201 tacggacaca accaaaagat ttggcaaaga agatttcttg ttcctctgtt ggatagattc
     1261 tgaatttgta tgcttttagt tgttccataa agttcaccta ctttctattt atgattgttt
     1321 ccttgatttt ggatatactt cttaatcaca tcaattggtg cgcc
//
LOCUS       DAYYCY010000282         1355 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.289, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000282 DAYYCY010000000
VERSION     DAYYCY010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.289"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1355
                     /locus_tag="ACHAZB_002785"
     rRNA            <1..1355
                     /locus_tag="ACHAZB_002785"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          362 a          295 c          415 g          283 t
ORIGIN      
        1 caagctgtga tggggaggga aataatagta ccgaagttcc tgatgtcaca ctgccgagaa
       61 aagcttctag tgagaaaaca gcggcccgta ccgcaaaccg acacaggtag tcgaggagag
      121 aatcctaagg tgagcgagag aactctcgtt aaggaactcg gcaaaatgac cccgtaactt
      181 cgggagaagg ggtgctgatc atacgatcag ccgcagtgaa taggcccaag cgactgttta
      241 tcaaaaacac aggtctctgc aaaatcgtaa gatgaagtat aggggctgac gcctgcccgg
      301 tgctggaagg ttaagaggag tgcttagcgc aagcgaaggt acgaattgaa gccccagtaa
      361 acggcggccg taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga
      421 cccgcacgaa aggcgtaacg atttgggcac tgtctcaacg agagactcgg tgaaatttta
      481 gtacctgtga agatgcaggt tacccgcgac aggacggaaa gaccccatgg agctttactg
      541 tagtttgata ttgagtgtct gtaccgcatg tacaggatag gtaggagccg tagaaatcgg
      601 aacgctagtt tcgatggagg cgctggtggg atactacccc tgcgttatgg ccactctaac
      661 ccgcaccact aatcgtggtg ggagacagtg tcagatgggc agtttgactg gggcggtcgc
      721 ctcctaaaag gtaacggagg cgcccaaagg ttccctcaga atggttggaa atcattcgaa
      781 gagtgtaaag gcagaaggga gcttgactgc gagaccaaca agtcgagcag ggacgaaagt
      841 cgggcttagt gatccggtgg ttccgcatgg aagggccatc gctcaacgga taaaagctac
      901 cctggggata acaggcttat ctcccccaag agtccacatc gacggggagg tttggcacct
      961 cgatgtcggc tcgtcgcatc ctggggctgt agtcggtccc aagggttggg ctgttcgccc
     1021 attaaagcgg cacgcgagct gggttcagaa cgtcgtgaga cagttcggtc cctatccgtc
     1081 gcgggcgttg gaaatttgag aggagctgtc cttagtacga gaggaccggg atggacttac
     1141 cgctggtgta ccagttgttc tgccaagggc attgctgggt agctatgtag ggaagggata
     1201 aacgctgaaa gcatctaagt gtgaagccca cctcaagatg agatttccca tttctttaag
     1261 aaagtaagat ccctgagaga tgatcaggta gataggtcag gagtggaagt acagtgatgt
     1321 atggagcgga ctgatactaa tcgatcgagg actta
//
LOCUS       DAYYCY010000283         1339 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.290, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000283 DAYYCY010000000
VERSION     DAYYCY010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1339
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.290"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1309
                     /locus_tag="ACHAZB_002786"
     CDS             <1..1309
                     /locus_tag="ACHAZB_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional aminoglycoside N-acetyltransferase
                     AAC(6')-Ie/aminoglycoside O-phosphotransferase
                     APH(2'')-Ia"
                     /protein_id="HFM8615391.1"
                     /translation="SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHY
                     PKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIR
                     AYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDS
                     IEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEY
                     SYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISEC
                     TIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCN
                     HLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEK
                     AKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          569 a          102 c          226 g          442 t
ORIGIN      
        1 atcattaaaa aaacattata cagagccttg ggaagatgaa gtttttagag taattattga
       61 atataacaat gttcctattg gatatggaca aatatataaa atgtatgatg agttatatac
      121 tgattatcat tatccaaaaa ctgatgagat agtctatggt atggatcaat ttataggaga
      181 gccaaattat tggagtaaag gaattggtac aagatatatt aaattgattt ttgaattttt
      241 gaaaaaagaa agaaatgcta atgcagttat tttagaccct cataaaaata atccaagagc
      301 aataagggca taccaaaaat ctggttttag aattattgaa gatttgccag aacatgaatt
      361 acacgagggc aaaaaagaag attgttattt aatggaatat agatatgatg ataatgccac
      421 aaatgttaag gcaatgaaat atttaattga gcattacttt gataatttca aagtagatag
      481 tattgaaata atcggtagtg gttatgatag tgtggcatat ttagttaata atgaatacat
      541 ttttaaaaca aaatttagta ctaataagaa aaaaggttat gcaaaagaaa aagcaatata
      601 taatttttta aatacaaatt tagaaactaa tgtaaaaatt cctaatattg aatattcgta
      661 tattagtgat gaattatcta tactaggtta taaagaaatt aaaggaactt ttttaacacc
      721 agaaatttat tctactatgt cagaagaaga acaaaatttg ttaaaacgag atattgccag
      781 ttttttaaga caaatgcacg gtttagatta tacagatatt agtgaatgta ctattgataa
      841 taaacaaaat gtattagaag agtatatatt gttgcgtgaa actatttata atgatttaac
      901 tgatatagaa aaagattata tagaaagttt tatggaaaga ctaaatgcaa caacagtttt
      961 tgagggtaaa aagtgtttat gccataatga ttttagttgt aatcatctat tgttagatgg
     1021 caataataga ttaactggaa taattgattt tggagattct ggaattatag atgaatattg
     1081 tgattttata tacttacttg aagatagtga agaagaaata ggaacaaatt ttggagaaga
     1141 tatattaaga atgtatggaa atatagatat tgagaaagca aaagaatatc aagatatagt
     1201 tgaagaatat tatcctattg aaactattgt ttatggaatt aaaaatatta aacaggaatt
     1261 tatcgaaaat ggtagaaaag aaatttataa aaggacttat aaagattgat tatataatat
     1321 atgaaaagct attataaaa
//
LOCUS       DAYYCY010000284         1333 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.291, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000284 DAYYCY010000000
VERSION     DAYYCY010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1333
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.291"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(72..692)
                     /gene="rpoE"
                     /locus_tag="ACHAZB_002787"
     CDS             complement(72..692)
                     /gene="rpoE"
                     /locus_tag="ACHAZB_002787"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HFM8615392.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            complement(768..1205)
                     /locus_tag="ACHAZB_002788"
     CDS             complement(768..1205)
                     /locus_tag="ACHAZB_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HFM8615393.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTGGKVRIDYDLFMGPEKIGEYYLTLDFTA"
BASE COUNT          348 a          318 c          196 g          471 t
ORIGIN      
        1 aaatcgtgtc acacctcctt attctgatag gctgatcgtc tctgtcacaa cctctcgtaa
       61 gaaaataatg tttattcttt ttcagagtca gaaaattctt cctcttcata atcttcatca
      121 tcatcgtctt cgtctatgat acttaaatct tcttcgatat ttcctggcaa gtcttcttca
      181 tcatctgtcg ggtcatctgc tccgatttct tgcaagtcag aattataagc agcgatttct
      241 tcatcttcat catcatcgtc atcaaacaga tcatcgtcat cttcttctgt cagatctgta
      301 tcttctgggt catcgtcatt gtaatcgatc gcgtcttcat catcattgat aaaggcgttg
      361 actttcttgc gtttacggcg acgtggagta tcttcatcct cttcgtccat tccatggttt
      421 acttcttcat cgatcgaatc gatagcatac catgaacgca agccccaacg attatcccct
      481 aatgagataa agctgccgtc aatattcaag tcagtataaa attgtgcgag tgaatcacga
      541 atttcactgt ctgatttccc aagatagttt tggatctggt tagctaaatc agaaaaatcc
      601 atgacatcgc cacgttgttc taagatcgcg tgtgctactt cgatcattga taattcgctt
      661 ttgtttgcac cttcaaatac actaatttcc aatcaggaca cgtcctttca cagtctactc
      721 cttatcatac aaaatcagac acaaaaaatc aaacttttct tttatattta cgctgtaaaa
      781 tccaaggtca aataatattc gccgattttt tccggtccca tgaacagatc ataatcgatt
      841 cgaaccttcc ctcctgtggg acggtctttc aaactaacat gcagttcttt cgtaaaagta
      901 gcaatctgga tcataccgta tggtgttcga taggatgttt ctaaccgttc tttatagcca
      961 aatttcaaac gcatccgcat ttctcctgca cggatcagct gtacatgacc atctggaact
     1021 aatttgatcg ttacaggaat ctcatcgccc gtctcctcct gaatctcttt ataacgtatg
     1081 tacagcgtat cgccaattgt caccatttgc cctttcaaat caaaataaaa atcttgcact
     1141 tccccttgct gttttacttt tgtacgcaat ttgattgaaa tcggtgttcc ttgtgataat
     1201 tccattattc attctccttt acttctcata gctacattgt tcctagtttc ttttgttttg
     1261 tcaaccaact tgtccatttt tacagaaaac cttttttcca aaacaactct ttgattttag
     1321 agtataatat tgt
//
LOCUS       DAYYCY010000285         1296 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.292, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000285 DAYYCY010000000
VERSION     DAYYCY010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1296
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.292"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..591)
                     /locus_tag="ACHAZB_002789"
     CDS             complement(<1..591)
                     /locus_tag="ACHAZB_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFM8615394.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQA"
     gene            942..1241
                     /locus_tag="ACHAZB_002790"
     CDS             942..1241
                     /locus_tag="ACHAZB_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFM8615395.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT          470 a          186 c          213 g          427 t
ORIGIN      
        1 tgcttgcaac tgattaagcg tacaattttt tagatgttcc gagcgtactt caatcatttc
       61 aaaggagttg ctacttaaat cgatattttt taaatcagta tcaaaaaagc tagcattttc
      121 taaatcatta gtagaaaaca ttattttagt gagggaacag tctacaaaat ctgcgcttga
      181 taagacagag tgctcaaagg tactttcttt tatgtttgaa ccagataaat ttacatagtc
      241 cattttacaa ttctcgaata acgtgttccc aatataactt tccatcaatt tagtacctgt
      301 taatttacaa ttgacaaatt tcacgcgatg caacatgctt tttgtcaata tacagcctga
      361 aaaatcacag ttttcgaata tacagtcggt aatctccatt ctttgaaaat cattggaagc
      421 aaaaaatgca tacttgaaaa tagatttgta aataattgga tggcagatta tttggttttc
      481 gatgacctct ttttgtgaaa ggatattata cagttcaaaa tcttcgtttt catagatatg
      541 aaaaaaggat cctttattca agcttgcttc attaacttta ggaaaaatca tatttaatta
      601 cctccatttt cataaagtca tattaacaaa agctaaacca acttacatta gcagaaaatg
      661 agataggttt tcgccttttt cgataacgat agtgtcgcta tctcttgaaa cgttcttaga
      721 attgccctga gagacacaaa ttaggcgtaa ttctaataaa aatcttagaa taaaaatttt
      781 tactgattta atttagaatg tttttaaaat aaaaatcgtt gccataacag tgtattgaaa
      841 ggttttaaca atccagtaat gatttaaaaa aatctagatg tagcgtatac tttgtttata
      901 gaatgtagtg ggaagaaata tatttttggg aggaaatcgt gatgtctaaa agtgaagaaa
      961 tagaaaacat ggtactaaaa ttatatgatc tcgttttaaa tccagagatc aaagaaaagg
     1021 aacgtatact tttgatagac gcaaagacag gcttagaaaa aggtcagtat tatcctaaag
     1081 tcataaataa tttggaacgt agtttacgtc cgttagcaat caggggagaa ttgtctaaac
     1141 cagtatcacc gttttatatg gagatatcaa caataggaaa atttgaaaaa gaattaggtc
     1201 gaggaatggc ttcggcaccg attacttttg ggcatttata aaaacgagtt attcatctca
     1261 atgaattaaa aagtatagtc aatctcataa tgcagg
//
LOCUS       DAYYCY010000286         1277 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.293, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000286 DAYYCY010000000
VERSION     DAYYCY010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1277
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.293"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..238
                     /locus_tag="ACHAZB_002791"
     CDS             <1..238
                     /locus_tag="ACHAZB_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFM8615396.1"
                     /translation="QQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILD
                     IDSEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            243..1190
                     /locus_tag="ACHAZB_002792"
     CDS             243..1190
                     /locus_tag="ACHAZB_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFM8615397.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          419 a          184 c          266 g          408 t
ORIGIN      
        1 gcaacagaca cctgaagttt ttgaaatgaa tgcctcaatg tattctttta aacctgaatt
       61 tctaaaaaaa agtaattata tttttgatgg gcaatgtggg ataataaaaa tgagagatta
      121 tctaatttta gatattgata gtgaagaaga ttttaaatgg ttagaatttt tgtttcctaa
      181 atttttagaa gattcttcgg agcttcaaga tatctataat tatttatatc ggaggtaaaa
      241 ggatgagctg ttatcttatc accggtgggg ctggcttcat tggttctacc ttagccaatc
      301 atttaggaga agaacatgtg gtaatcgtcg tcgatgactt atcaatggga aaagtagaaa
      361 atcttgatat gacgaaaaat atcactttta ttgagggaga tgtcgctgag aaatccttga
      421 tggaagagat catgaaagct aataaatttg attatatctt tcatttagca gcagttgcta
      481 gtgtggcaga ttcagttgcc cgaccagtag aaacacatcg tgtgaatttt gaaagtgtat
      541 taatgatttt agaattgatc cgcaagtatc aacctgattt aaaacggatc gtgtttagtt
      601 cttcagcggc tgtttatggg gatgaaccga ctttaccaaa aaaagaagag tccgtgattc
      661 gcccattgac gccttatgcg gttgataaat tcgctgcaga acaatatgtg ctagattatt
      721 gtcatttata tgatgtaccc ggtagcgcag ttcgtttttt caatgtctat ggtccaaatc
      781 aaaatccgaa ttctccatac agtggtgtga tttctattct cgtagatcgc tataagaaac
      841 aaatcgtagg agaagcaact tcatttacat tatttggtga tggaagccag tctagagact
      901 ttgtttatat agatgatgta atccaagcat tacttttagt tgcgaatgag gaagctgcac
      961 tggggaaaca attcaatgtt ggaacgggta aagcaaccac acttttggaa ttgattcatg
     1021 caatcaatga aattctcggt gtagaattga cacttgaata caaagaagag cgttcgggtg
     1081 atatccatga ctctctagca gatattacta agatccagtc aattggctat caaccgaaat
     1141 atggtatttt aagtggaatg actaattatt tgaaaactga aatcaattaa aacatactaa
     1201 gataagatta gagggtaaat tcaggctctt tgtcaataag gactgatgag ttttgtaaaa
     1261 tcaaatctgg gatagaa
//
LOCUS       DAYYCY010000287         1277 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.294, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000287 DAYYCY010000000
VERSION     DAYYCY010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1277
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.294"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            210..>1277
                     /locus_tag="ACHAZB_002793"
     CDS             210..>1277
                     /locus_tag="ACHAZB_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8615398.1"
                     /translation="MSVKKMLFMVSTIIVLTSRFWGINTSLSMRYFIMAIWDLYFMLS
                     YFSYREKTYKKYVIKNNFWLTIMPFVIFSIYTLIIWGIGSNAEFENFIKLCSTLLYLI
                     LAYGYACTAFYLFKAEGIDFIFWSGVISYMSGSVFYLIVSYGLNGLISYIKSLITGET
                     NTANFAMEVHDLTFAMGVFFLYYVFFENKNTKKHKIKIIVSLILIFMGLKRIELLALS
                     GAILVYYVLLKWGKKIQIRALVCTICATVISLVFIYIIDTGILALLMSNLGITDDGAR
                     LSFYLYSSKFYELGLGYVGTGWTWFSKYFFNLYLSHFRLNGGRIAASLHSDILTIYIE
                     VGCIVFIIWMFWLFFVKPIRLT"
BASE COUNT          421 a          129 c          221 g          506 t
ORIGIN      
        1 tgaaatttct atatacaatg agaagctgaa atttactttt atttgatgac tggaaataga
       61 aattaaatat attgtattgt tacattaaag aaaacttgta tggaaaaatc agtacaatac
      121 cattatttaa tgttcttcac ttaaagcgca aaaaatatca gtttattaaa ctcgtatata
      181 gaacaaatat atgtagacgg ggcgaaacga tgagtgtaaa aaaaatgcta tttatggtaa
      241 gtacaataat tgtattaaca tcccgatttt ggggaattaa tactagttta tctatgcgat
      301 attttattat ggcaatatgg gatttatatt ttatgttaag ctatttttct tatcgtgaga
      361 aaacatataa aaaatacgtt atcaagaata acttttggtt gactataatg ccgtttgtga
      421 tattttctat ctatactcta ataatatggg gaattggttc aaatgcagaa tttgaaaatt
      481 ttataaagtt atgttccacg ttgctttatt taattcttgc atatggttac gcatgtacag
      541 cattttactt atttaaggca gaaggaattg attttatttt ttggtcaggt gttatttcat
      601 atatgagtgg ttcagttttt tatttaattg tatcttatgg tttaaatggt ttaatttctt
      661 atataaaaag tcttataaca ggtgaaacaa atactgcaaa ttttgctatg gaggtacatg
      721 atttaacttt tgcgatgggg gtttttttcc tttattacgt attttttgaa aataaaaata
      781 caaaaaaaca taagataaaa attatcgtat ctttaatttt aattttcatg ggattgaaga
      841 gaattgaact gttagcttta agtggtgcaa ttctagtgta ttatgtttta ttaaagtggg
      901 gaaaaaagat tcaaatccgt gcattagtgt gtactatttg tgcaactgtt atttcgttag
      961 tgtttattta cattatagat acaggaattt tggcattgct tatgtcaaat ttgggcatta
     1021 cagatgacgg tgcgaggctt tcattttatc tatattcatc taaattttat gaacttggtt
     1081 taggatatgt aggaacagga tggacatggt tttcaaaata cttttttaat ctttatttat
     1141 cgcactttag gttaaatggt ggaagaattg cagcaagcct acacagtgat atattaacta
     1201 tttatataga agttggatgt atagttttta ttatatggat gttttggtta ttttttgtga
     1261 aaccgataag actaact
//
LOCUS       DAYYCY010000288         1265 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.295, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000288 DAYYCY010000000
VERSION     DAYYCY010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.295"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            20..1099
                     /locus_tag="ACHAZB_002794"
     CDS             20..1099
                     /locus_tag="ACHAZB_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM8615399.1"
                     /translation="MTQTHDNTVSRKGKHLSYSERCQIAILKKEGYSNRQVAKVLGRV
                     PQTINNEIDRGTITQLKRQTQNGKVYDTYYSVYDADAGQAFYEKQRLNCGRRPKWANT
                     NAFIDWADDKMLNEKWSPDVVVGFARKHELFDCAIIPGTTALYQWIDRGIMRTKNMDL
                     LEKLSRKIKAVRPGHRPNKRVLGTSIEERPKEVETRETFGHWEIDTVVGNKTKSDAVL
                     LTLAERQTRFEVILKLNGKDTESVDQAVQQLRERAGANFDQLFKTVTSDNGSEFSGLH
                     DALQDVADVYFSHPYASWERGTSENQHKLIRRFVPKGSPISEVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFKQERLVA"
BASE COUNT          376 a          301 c          310 g          278 t
ORIGIN      
        1 tagacaagga tgaattcaca tgacgcaaac ccatgataac acagtttcac gcaagggaaa
       61 acatctttcc tactcagaac gttgccaaat cgctatcctg aaaaaggaag gctattccaa
      121 ccgtcaggtt gccaaagtgc tgggacgtgt tccgcaaacc ataaacaatg agattgatcg
      181 cggaaccatc acccagttaa aacgccaaac gcaaaatgga aaagtgtatg acacctacta
      241 ttctgtttat gacgctgatg caggacaggc cttctatgaa aagcaacgac tgaactgtgg
      301 cagacgcccc aaatgggcga acacaaatgc cttcattgat tgggcagatg acaaaatgtt
      361 aaatgaaaaa tggtcgccgg acgtcgtggt cggatttgcc agaaaacacg agctgttcga
      421 ttgtgccatc attcctggca caaccgctct ttatcaatgg attgaccgcg gaatcatgcg
      481 aacaaagaac atggacttgc ttgaaaaact gtcccgtaaa atcaaagcgg tccgcccagg
      541 acaccgtccc aacaagcgtg ttctcgggac gtcaattgaa gaacgcccta aagaagttga
      601 aacacgggaa acgttcggtc attgggaaat cgacacggta gtgggaaaca agaccaagtc
      661 ggatgccgtg ctgctgacgc tggctgaacg gcagacacgc tttgaagtca tcctaaagtt
      721 gaacgggaag gacaccgaat cggtggacca agcggttcaa cagctaagag aacgggctgg
      781 cgctaacttc gatcaattat ttaagaccgt tacatccgac aatggctcgg agttttctgg
      841 gctacacgat gctctgcaag atgtagctga cgtctatttc agccacccct atgcttcttg
      901 ggagcgtggg acgagcgaaa accagcacaa attaattcgc cgtttcgtcc ccaagggaag
      961 cccgattagt gaggtgagcg agaaccagat tttgcggatc cagcgttgga tgaacgatta
     1021 cccgcggaaa attttgggtt atgcgacacc acatgataca ttcgtccagg cattcaagca
     1081 agaacggttg gtggcttgac ccattattcc ttgataacga tcctcactcg ggacatggct
     1141 tcatgtctgg aaagctggcc tggggccaga atcgtctgaa gactatcata cctcagttca
     1201 ttatcaaggc ctcactgatt tagaataacg aggcagttac ggtatcaaac aaatgagtta
     1261 cctgt
//
LOCUS       DAYYCY010000289         1261 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.296, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000289 DAYYCY010000000
VERSION     DAYYCY010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1261
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.296"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            20..1099
                     /locus_tag="ACHAZB_002795"
     CDS             20..1099
                     /locus_tag="ACHAZB_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM8615400.1"
                     /translation="MTQNHDNTVSRKGKHLTYSERCQIAILKKENYSNRQVADVLGCV
                     PQTINNEIKRGTVTQLNRQNQNGKVYDYYHDVYDADAGQAAYEKNRLNCGRRPLWADT
                     DAFIEWADDMMLLEKWSPDVVVGFARKHELFDPAIIPGTTTLYQWIDRGIMKTKNMDL
                     LEKLSRKPKSSRHGNRPNKRVLGKSIEERPEAVGTRKTFGHWEVDTVVGNKTKSDAVL
                     LTLVERQTRFEVILKLAGKDAESVNQAVRQLRERAGAYFGQLFKTVTSDNGSEFAGLH
                     DALQDVANIYFSHPYASWERGTSENQHKLIRRFIPKGSPIGNVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFRQERLTA"
BASE COUNT          333 a          342 c          356 g          230 t
ORIGIN      
        1 tagacaagga tgaattcaca tgacgcaaaa ccatgataac acagtttcac gcaagggaaa
       61 acacctaacc tactcagaac gttgccaaat cgccatcctg aaaaaggaaa actattccaa
      121 ccgtcaggtt gcggatgtcc tgggctgcgt gccgcagacc ataaacaacg aaatcaaacg
      181 cgggaccgtt actcagctta accgccagaa tcagaacggg aaggtctacg actattatca
      241 tgacgtctac gacgccgatg ccgggcaggc ggcctacgag aagaaccgct tgaactgcgg
      301 cagacggccc ttatgggccg acacggacgc cttcattgaa tgggcggacg acatgatgct
      361 gttggaaaaa tggtccccgg acgtggtggt gggctttgcc agaaaacacg aattgttcga
      421 cccggccatt atccctggga ccaccaccct ctatcagtgg atcgaccgtg gcatcatgaa
      481 aacgaagaat atggacctgc tagaaaaact gtcccgcaaa ccgaaatcgt cccgtcatgg
      541 caaccgcccg aataaacggg tgctagggaa atccattgaa gagcgcccgg aggcggttgg
      601 tacccggaaa accttcggcc attgggaagt cgacacggtc gtaggaaaca agaccaagtc
      661 ggatgccgtg ctgctgacgt tggtggaacg gcagacacgc ttcgaggtca ttctgaagct
      721 ggccgggaag gatgccgaat cggtcaacca agcggtccga cagctgagag aacgggctgg
      781 tgcgtacttc ggccagctgt tcaagacggt tacctcggac aacggctcgg agttcgcggg
      841 actgcacgat gccttgcagg atgtggcaaa catctacttc agccacccct atgcttcctg
      901 ggagcgcggg acgagcgaaa accaacacaa gctgattcgc cgtttcatcc ccaagggcag
      961 cccaatcggc aacgtgagcg aaaaccaaat cttgcggatt cagcgttgga tgaacgatta
     1021 cccgcgaaag attttgggct atgcaacgcc gcatgacacg ttcgtccagg catttaggca
     1081 ggaacggttg acggcttgac acgtgggccc ttgacaacga gcctccctcg gcatggcttg
     1141 tgtctggaaa gctggccggg gccagccagt cgggagacta ccatgcctca gctcattgtc
     1201 aagggcccac tcgattagaa aaattagacg gttggtggta acgagcaacc gggtcaccat
     1261 g
//
LOCUS       DAYYCY010000290         1246 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.297, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000290 DAYYCY010000000
VERSION     DAYYCY010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.297"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            200..742
                     /locus_tag="ACHAZB_002796"
     CDS             200..742
                     /locus_tag="ACHAZB_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8615401.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            898..>1246
                     /locus_tag="ACHAZB_002797"
     CDS             898..>1246
                     /locus_tag="ACHAZB_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HFM8615402.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMP"
BASE COUNT          514 a          141 c          238 g          353 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccag
//
LOCUS       DAYYCY010000291         1215 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.298, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000291 DAYYCY010000000
VERSION     DAYYCY010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.298"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            414..947
                     /locus_tag="ACHAZB_002798"
     CDS             414..947
                     /locus_tag="ACHAZB_002798"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFM8615403.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT          389 a          226 c          225 g          375 t
ORIGIN      
        1 cttattaccg cagatgcaag cttttgaaca ccgtttattg agaagttgtg aaatcagcgt
       61 cctgacctga gtattagaga aacaaatggc aaaatagttc ctcatatttt gctatttttt
      121 gagctaatac cgagggtcag ctgattgaac accgtttagt ggagaagttg tgaaaaagtc
      181 gcctactttc gagtcgtaag aaattttttt gaaaatggtt cctcacaact ttgcataaat
      241 ttgacttaat accgagaagt catgatcccc atctaactcc aaatcacaag gaatgagttc
      301 aaaaactagc ttttctcaac aaatattaca taaaaaattc tatgtttttt gttaaattta
      361 tttgcttttt tcaaaagtat gttacagtaa tttcccaagg aggttttaca gaaatgaatt
      421 ttgttgccat ggattttgaa actgccaatc atcagcctta tagtgcttgt tctcttgcgc
      481 tagtcatggt aaaaaatagc cagatcgttg atgaattcta tacattgata cagcctgaga
      541 ctcctttttt ctggcgaaat gtccaaattc acggaatcca tcaagaagac gtacgcaatg
      601 cgccgaaatt cccagaagtc tggcaaacta tccaaccata ttttcaaaaa aatcgtttag
      661 tcgttgcaca taatgctgct tttgatacaa aggtcttagc gggatgctta gattattatc
      721 atcttgaaca gcctaattat ctttctttat gtacagtgaa aagtagccgt cgcctgtttc
      781 ctgaatttcc aaatcatcga ttgaacactg tgtgcgaaaa tttgaacatc caattagatc
      841 atcaccatga tgcattggaa gatagtcgtg catgtgcgga aatcctgctt tatcaagaaa
      901 aaaaattcgg gacagatcca ttgaaaaaac ttgtgactat aaagtaaaag aatacttatg
      961 aaacacacaa agaggtcgtg agcctgccgt ttcactccga gtcacaagga gcaatttcaa
     1021 aaaatagctt ctcatgttta ttaaatattg tgactggtga ccgaggagct ggcaggagaa
     1081 caccgtttat tggagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
     1141 acaaaaatag cttctcatat ttttcatttg gttggactta ttaccgcaga tgcaagcttt
     1201 tggacaccgt ttacc
//
LOCUS       DAYYCY010000292         1207 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.299, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000292 DAYYCY010000000
VERSION     DAYYCY010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1207
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.299"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..652
                     /locus_tag="ACHAZB_002799"
     CDS             <1..652
                     /locus_tag="ACHAZB_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HFM8615404.1"
                     /translation="IGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFIDS
                     PVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKVIE
                     KHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSRKS
                     LVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE"
     gene            649..>1207
                     /gene="neuC"
                     /locus_tag="ACHAZB_002800"
     CDS             649..>1207
                     /gene="neuC"
                     /locus_tag="ACHAZB_002800"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0006047 - UDP-N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HFM8615405.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPS"
BASE COUNT          400 a          160 c          261 g          386 t
ORIGIN      
        1 aattggctca ggagatttaa caaatttacc ttttcttaga aaaatagata tgtatgggtt
       61 gccagtttta ttatctacag gtatggctga tattcatgag gtcagtgagg ctgtagattg
      121 tttcatagat agtccagtta ctattttaca ttgtacttca aattatccgg ctagttctga
      181 agatattaat ttattggcga ttaacacatt aaaagaaatg ttcgggatac ccgttgggta
      241 ctccgatcat tcattgggat atgatgttgg tatttgtgct gttgcgatgg gtgctaaggt
      301 aattgagaaa cattttacct tggataagga actacctggt ccagatcata aagcttcttt
      361 agatcctcag gaatttgcag attttgtaaa gcatattaga aacactgaaa tatttttagg
      421 tgatggtgtt aagcgagcaa tgccttctga acttagtaca aaagaagtat caagaaaaag
      481 cctagtggtc actgagaatt taaatgtggg agatattctt acagagaaat ctcttacaat
      541 aaaacgtcct ggaaatggaa tagctcctaa gtttttatat caatatattg gcaaaaaagt
      601 taaaagacca ataagtgcag atagtgttct gcttgaggaa gatgttgaat gaaaaaaata
      661 cttatagtaa gtggaacacg agctgattat ggaatatata aacctgttgc tgagaattta
      721 ctacaagata attgggatgt atcatttgtt gtttctggaa tgcatttatc ccataaatat
      781 ggatttacta aagaattgat cttagctgac ggatttagaa ttagtggaga agttcaaagt
      841 ttgtttttag agaacaataa gggaaacatg gctcggtctg tggcattaga gataaatggt
      901 ttcacaaata tatttgaaca aactagccct gattttgtat tattactagg ggatagagga
      961 gaaatgttgg cagctgcgat agcttgtttg tatcttggaa tcaaaacagc acatattcac
     1021 ggaggcgaag tgtctggtac tgtagatgaa tcaataagac atgccataac gaaaatagca
     1081 tctgttcatt ttgctgctac ggaaaaaagc aaagagcgtt taataaaaat gggggaagat
     1141 tcttggagag tttttcccgt cggagctcct agaatagata ctattttaaa taatagatta
     1201 cctagtt
//
LOCUS       DAYYCY010000293         1183 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.300, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000293 DAYYCY010000000
VERSION     DAYYCY010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1183
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.300"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..153
                     /locus_tag="ACHAZB_002801"
     CDS             <1..153
                     /locus_tag="ACHAZB_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFM8615406.1"
                     /translation="NKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFSFSVSTEIKV
                     LMGITA"
     gene            207..575
                     /locus_tag="ACHAZB_002802"
                     /pseudo
     CDS             207..575
                     /locus_tag="ACHAZB_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297423.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            681..944
                     /locus_tag="ACHAZB_002803"
     CDS             681..944
                     /locus_tag="ACHAZB_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFM8615407.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            944..>1183
                     /locus_tag="ACHAZB_002804"
     CDS             944..>1183
                     /locus_tag="ACHAZB_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFM8615408.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVLLRSLKIKR"
BASE COUNT          448 a          154 c          239 g          342 t
ORIGIN      
        1 aataaatttt atcaaagtct ccgtacagcc tcttccacga ttaagggcat ggagaccatt
       61 cgaggaatat ataaaaagaa ccgaagaaat ggaacgctct tcagcttttc ggtgtctact
      121 gaaatcaagg tattaatggg aataacagcc taagatattt ggagttcaga gagggcgcgt
      181 ttgattttca aacttcgcaa cagaaccgaa aatttatacc ggaagatttt caaaacttac
      241 agttagaaaa catgcgttta gaagatttat ttagattagc agatgatgat aagtatagat
      301 ttgaagtttt gaaaattaaa aagaatgaac tattgaataa gttatatcct gagaatgcga
      361 taaaagcaca ttttggtgat gctaatcata tgtatagttg gttcattcct aaaagattta
      421 gagggaaaat caatgctgga gatcaagtga aagtactcaa tcgaaatagt acagatgtcg
      481 taatgataac agatatctac gtagaagatg aagttataat caaatatatg aagcctgtga
      541 taaaacttgt taataaaaat aaaggaaacg gataacactt tacaatgctg ttttggtact
      601 ccaatttaaa caaaacaatc gaatttacgt acttaattaa gtacgttaga atggagatat
      661 aaattagagg gggagtagat atggcaaaaa cagtcttaaa tcctagctca gcacggaaaa
      721 atttttataa gttgttgaaa gatgtaaatg agaatcatac ggaaattgaa atcattagtg
      781 agcgcagtga aaataatgcg gtgcttattg gtttggatga ttggaaagca atcaaagaaa
      841 cgctattact tgagcaaata ggcactttgg gtgttgttcg taagcgagaa aaagatgata
      901 gtggatttac taatgttgat gaaattgatt gggataactt ataatgaata actattctgt
      961 gatgattaaa aactcggcta aagctgattt aaagaaaatc aaacagtcta atttgaaacc
     1021 tcaatttgag aaggttattc agactttaaa ggaagatccc tatctcccta cccaatcatt
     1081 tgagaagtta aaacctacac atgaaggtag gtattctaga agattgaata gacaacaccg
     1141 ggttgtttat aaggttctgt tgcgaagttt gaaaatcaaa cgc
//
LOCUS       DAYYCY010000294         1178 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.301, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000294 DAYYCY010000000
VERSION     DAYYCY010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1178
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.301"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..996
                     /locus_tag="ACHAZB_002805"
     CDS             <1..996
                     /locus_tag="ACHAZB_002805"
                     /EC_number="4.2.1.115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050377 - UDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase family
                     protein"
                     /protein_id="HFM8615409.1"
                     /translation="KTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFYMQQE
                     LSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNVMGTQ
                     NVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIFSCVR
                     FGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSKFGET
                     YVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEIERSY
                     ENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVTW"
     gene            1008..>1178
                     /locus_tag="ACHAZB_002806"
     CDS             1008..>1178
                     /locus_tag="ACHAZB_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HFM8615410.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKY"
BASE COUNT          463 a          155 c          222 g          338 t
ORIGIN      
        1 aaaacaatat tagtaactgg cggaactggt tccattggaa gtgaaattgt tcgtcagctt
       61 ttaaaatatg aaccaaaagc tatcagaatt tatagtagag gtgaagaaaa acaattttat
      121 atgcaacaag agctctcaaa tcacactaat ttgagatttt taattggtga tatcagagac
      181 tctcaaagga ttgattatgc atgtcgtgat gtggatatta tttttcatgc tgccgcaatg
      241 aaacacgttc cagcaagtga atataatcct atggaagctg taaaaacaaa tgtgatggga
      301 actcaaaatg taatagatgc agcgatcaaa aataatgtaa aacattttgt aggtatcagt
      361 acagataaag tagtgagtcc tactaacacc atgggtgcga caaagttatt gagtgaaaaa
      421 ttaataatag ctgccaatca gtataaagga aaaacagaaa caatattttc ttgtgtaagg
      481 tttggaaatg tcatggggtc aagtggatct gttattcctc tttttagtaa acaaataaaa
      541 aacaataaag atattacttt gacagatgtt gatatgacac gatttatgat gtcaataccg
      601 gatgctgcaa gattagttct gtctgctgct atgaattcaa aatttggaga aacatatgtc
      661 ttaaaaatgc cggcaatcaa tattttaact ttagctcaat cattaataaa aatatataat
      721 gatacacatg atgaaaaata tagaggtgaa ataaaagttg taggtatcag gccaggagaa
      781 aaattatatg aagagctaat gactacagac gaaattgaac gctcatatga aaatgataaa
      841 atgtatatca ttccaccaca ctatcatgct actgacgaac tgtacaaaga ttatgaaaaa
      901 attaaaatca ctgcatactc ttcagatgca acagaattaa tgagtggaca ggaatttatt
      961 agaatcgcaa aagagaataa tgtcgtgact tggtaaggag ttttatgatg aaatcagaag
     1021 tatatgtaat tgcagaaatt ggtgtaaacc ataatggaag tctagattta gctttagaaa
     1081 gtattgataa agctaaagaa tgtggagcta acgcggttaa atttcaaaca tttaaaacga
     1141 ataagctagt ttccaaaaag gctccaatgg caaaatat
//
LOCUS       DAYYCY010000295         1175 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.302, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000295 DAYYCY010000000
VERSION     DAYYCY010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1175
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.302"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>1175
                     /locus_tag="ACHAZB_002807"
     CDS             <1..>1175
                     /locus_tag="ACHAZB_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HFM8615411.1"
                     /translation="QKKKTLVVDAVLSPTPRACRSCGSTVVDGNGKAIIVKNGKKETI
                     VRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQSYFVQPRHSIANHVRYKIASLLTEKV
                     SLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSKKILPRVLMVDEFRSHASIEDKMSFIC
                     ADGETGKLIDVLPTRKLPRLTSYFLGCTNPEEVEFLVTDMNAAYFQLTKRVLPNAKVV
                     IDRFHIVKHMNQAFNELRIREMNELRKAGQKSQAEKLKKNWRFLLKNRANINHYEYKT
                     WKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAYPFFHELVEAFRDKDPDLFFSLLAELP
                     ETLDDSFREKLQNLLTYEEGITNAMIYPYSNGKIEAKNTHIKTMKRVSYGFKSFENM"
BASE COUNT          387 a          235 c          228 g          325 t
ORIGIN      
        1 ttcagaagaa aaagacgttg gtcgtcgatg ctgttctctc gcctactcct cgtgcttgta
       61 gaagttgtgg ttctactgtg gtagatggaa acgggaaagc aattatagtg aaaaatggaa
      121 aaaaggaaac gattgtccgt tttgaacaat acaatcatat gcctttggtt atgcgcctaa
      181 aaaagcaacg ctatacctgt aaaaactgtc gcacccattg gacggctcaa agttattttg
      241 tccaacccag acattcaatc gcaaatcatg ttagatataa aattgcttct ttactgactg
      301 aaaaagtatc tttatctttt attgcgaaaa gctgtcaggt atctttgacc accgttattc
      361 gtacattgaa agagtttaaa agctatttac caaagcaatc taagaagatc ctccccagag
      421 tattgatggt tgacgaattt cgttcgcatg cttccataga agataagatg agctttattt
      481 gcgcggatgg cgaaacagga aaattaatag atgttttgcc tacgcgtaaa ttacctcgat
      541 taacaagcta tttcttaggt tgtaccaatc cagaagaagt agaattcttg gtgacagaca
      601 tgaacgccgc ctacttccag ctcaccaaac gtgttctgcc aaatgcgaaa gtcgtgattg
      661 atcggtttca tattgtcaaa cacatgaatc aagcgttcaa tgagttgcgt atccgtgaaa
      721 tgaatgaact tcgtaaagcc ggacagaaaa gccaggcaga aaaactgaaa aagaactggc
      781 gctttttgct aaaaaatcgt gcaaacatca accattatga atacaaaaca tggaaaagtt
      841 tccgagcacc aaaataccca tttcttactg aagcaatgat gattgatcga ttgcttgagt
      901 tttctccgcc cctaaaggag gcgtatccct tttttcatga attagttgaa gcctttcgag
      961 acaaagaccc tgacttattt ttctccttat tggcagaact tcccgaaacg ttagatgaca
     1021 gctttcggga aaagcttcaa aaccttctga cctatgaaga aggcatcacc aacgcaatga
     1081 tctatcctta ttccaatgga aaaatagaag cgaagaatac ccacataaag acaatgaaac
     1141 gagtatccta cggatttaaa tcatttgaga acatg
//
LOCUS       DAYYCY010000296         1158 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.303, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000296 DAYYCY010000000
VERSION     DAYYCY010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1158
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.303"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..838
                     /locus_tag="ACHAZB_002808"
     CDS             <1..838
                     /locus_tag="ACHAZB_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HFM8615412.1"
                     /translation="SFSIVHNQVYFGGGINLWLAKKNGIRQRIAHSHATEDGKSQNIV
                     MNVLRKFLTKLLLQNATDYLAVSQEAGESLFQNHPFEIVHNGIDLELYSKNSEAKVNK
                     RKELDISMSTFVVGNIGRLEKQKNQSYLLEIFLEILKMNSDSYLILVGEGSLRNKLKA
                     KAVELDIEKRVKFLGERNDIPELLSVMDVFVMPSLYEGLPMVGLEAQAAKLKLVLADT
                     ISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYANKITQELKSYDVSYTTEQMESIYSR
                     DH"
     gene            871..>1158
                     /locus_tag="ACHAZB_002809"
     CDS             871..>1158
                     /locus_tag="ACHAZB_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HFM8615413.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQAEINLVKRALQNEKYLKYVLLSGA"
BASE COUNT          445 a          127 c          214 g          372 t
ORIGIN      
        1 atctttttct atagttcaca atcaggttta ttttggcggg ggaattaatt tatggttagc
       61 caaaaagaat ggaatcagac agagaattgc tcatagccat gcaactgaag atggaaaatc
      121 tcaaaatatt gtaatgaatg tattgcgtaa atttttaacc aaattgttgt tacaaaatgc
      181 aacagactac cttgccgtta gtcaagaagc tggtgaaagt ctgtttcaaa accatccctt
      241 tgagatagtt cataatggta ttgatttaga gctttatagt aaaaattcag aagctaaggt
      301 taataagaga aaagaattgg atatctcaat gagtactttt gttgtgggaa acattggacg
      361 attagagaaa caaaaaaatc aatcttattt gttagagatt tttttagaaa tactaaaaat
      421 gaattctgat tcttacttga ttttagtagg tgaagggtca cttagaaata aactgaaagc
      481 caaagcagta gaattggata tagagaaaag agtaaaattt ttaggagaaa gaaacgatat
      541 tcccgagtta ttatctgtga tggatgtttt tgtaatgcct tctctttatg aaggtctgcc
      601 aatggttgga ttagaagcac aagcagcaaa attaaaatta gtcttagcag atactatttc
      661 caaagataca aagttaacaa ataatgtgga ttttgtatca ttagatgaaa caccacaaga
      721 atgggcaaaa aaagtaataa aagagccgta tgctaataaa ataacacaag aactaaagag
      781 ttatgatgtt tcgtacacta ctgaacaaat ggagagtata tatagtagag atcattaaaa
      841 atgttcaaga tattttagga gtgattattt ttgaaaatat gttatttgat attggcatac
      901 aatgatattg gcaacttaaa gagattggtc aatcgattga acgaaaatgc agtttttatt
      961 attcatatag ataaaaaaac tgatatagaa ccatttattg aagcatttaa atcatatgat
     1021 aatgttgaat ttttatgtaa taaaaaccgt gttaaaattt attggggtgg tttttccatt
     1081 gtacaagcag aaatcaattt agtaaagaga gcattacaaa atgagaaata tttaaaatat
     1141 gtattattat ctggtgct
//
LOCUS       DAYYCY010000297         1143 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.304, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000297 DAYYCY010000000
VERSION     DAYYCY010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1143
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.304"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            109..1137
                     /locus_tag="ACHAZB_002810"
     CDS             109..1137
                     /locus_tag="ACHAZB_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8615414.1"
                     /translation="MYWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWIS
                     LLRISLQRMKQGHSITYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTE
                     QAYLYFMFGVMNTYLEEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLY
                     VNLAMIHLYSRVFGTKKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNT
                     YYEKNRRLIFQYCMLVRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYS
                     ASADQLPDIVISDYPIDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSI
                     LEKLDFVN"
BASE COUNT          426 a          172 c          210 g          335 t
ORIGIN      
        1 ttaagagaac ttaatactat tttgaaaaac tatcatttat cttgcagctt gaaaaaaggt
       61 aatccgatag aaggagaaga ataccgtatc cgatactttt ttcacttgat gtattggcag
      121 tcattcgacg aaaatcattc tgatatatta tcaagtataa aaaataaacg tcaactatta
      181 gaaaaatttg cgaaacatgc gccatatacg cgtagtatag atagaaagaa atggatcagc
      241 ctactaagaa tttcgttgca acgaatgaaa caaggccact cgattacgta tgtcccagaa
      301 gagatgaaca aatttgtgca tccattgata acaaaagaag agtttacgca ttacatacta
      361 gagccttttt ttgaagcaaa cttctggtcg atgaatcaca tcactccaac agagcaagct
      421 tacctttatt ttatgtttgg tgtaatgaac acctatttgg aagaagattt gttggatact
      481 ccagataaag tacaagaaag ttggggctgg attgaatcgt ctattgtaga aaattttgaa
      541 aaaaacttca agatttgttt tacaacttca gaaaaaaatt atctatatgt caatttggca
      601 atgatccatt tgtattcaag ggtttttggg acaaaaaaga aggtggatgc ttttggaaaa
      661 tcagcagaag acagagattt tcagcatata tttccagtga tgtatccaat catgcaacag
      721 ttttttagtg gggtagctcg cagaattcca gagcttaata cttattatga aaagaacaga
      781 cggttgattt ttcaatactg tatgttagtc agggatattt ttataaaaca cgaacaacct
      841 gtcatattct atattcaatc aaagtatggt aaaacacagg aattatgggc aaagaaaagg
      901 atattatcta tgactgaacg cccaataaaa tatagtgcat ccgctgatca gttacctgat
      961 attgtcatta gcgattatcc tatcgacaaa aaggcatatg atcaaacgaa tacacagata
     1021 ttttactgga atggacaacc tacaaaaaat gattgggcgc gtctgaaagc tgaaatcata
     1081 aaaatcagga atgagaaaaa tagatccata ttggaaaaat tggatttcgt taattaagaa
     1141 aag
//
LOCUS       DAYYCY010000298         1133 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.306, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000298 DAYYCY010000000
VERSION     DAYYCY010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1133
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.306"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(18..923)
                     /gene="xerC"
                     /locus_tag="ACHAZB_002811"
     CDS             complement(18..923)
                     /gene="xerC"
                     /locus_tag="ACHAZB_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFM8615415.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
BASE COUNT          336 a          231 c          194 g          372 t
ORIGIN      
        1 tgttcttttc cttattttta cgctcttgga tggaaagaac gatagttttt ttgtaaactt
       61 tcttttgtta catgggcata gatctgggta gtcgataaat tggcatgtcc tagcaattct
      121 tggactgtcc gcaaatctgc cccattattt aataaatgag tagcaaatgt atggcgcagc
      181 atgtgaggat ggatatcact atccaaactg ctttttttga tcatttgatt caagacatat
      241 tcaattccag taggcgtgat tggttttcca tgatgattca caaaaacgat atcatgctct
      301 tcgtgatatt ttgtcatcaa tacctggcgg ccttccctga aatattcttg cagagcatct
      361 tgagcaaaag aacctagagg agcatatctc tctttgttcc ctttaccatg gatcaacatg
      421 acctctgaat caaaatcaat gtctgatacc gacaaactgc tacactcact taaacggatg
      481 cctgacccgt aaaggacttc cagtaaagcg cggttcctca aatccaaagg actatctcct
      541 gctacgctgt caaaaagtgc ttgcatttca ttttcataaa aaaaacgtgg cagtctcaaa
      601 tttttctttt tgagatgaac gtaagaaaat ggattttctt ttatcacttc ttctttgaga
      661 agatactgat aaaacgaacg taggctggct atcttgcgac tgatagaatt tctgctatag
      721 ctaggacgat gatcagctaa tgcactcaaa tagacacgaa ccgtaaaata ttccgccttc
      781 agcagatcag cgtctccaga ttcctccaaa aaatgtttga aatggagaat gtcttcttca
      841 tatgcttctt tcgttttttc tgaatatcct ctttcgacaa tcaaataacg caaaaatttt
      901 tgttcccacg tggcttctgt cattttctca ccttcctttt gtcccagaga ataatttagc
      961 acagcgttta taaaaagaca aataaattgt cagaatttaa ccaatatttc taagtttgtt
     1021 cacaattggc gcaattcctc acattttgtt tgagaaattc ttaagaaaaa cgcaagaaaa
     1081 agggctctat aatttatggg actgatgaat cagaattgat tcttcagccc cat
//
LOCUS       DAYYCY010000299         1105 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.307, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000299 DAYYCY010000000
VERSION     DAYYCY010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1105
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.307"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1087
                     /locus_tag="ACHAZB_002812"
     CDS             <1..1087
                     /locus_tag="ACHAZB_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8615416.1"
                     /translation="DINDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTD
                     TRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINA
                     EFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKII
                     SLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVK
                     NDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKED
                     GELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISI
                     TMDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT          402 a          192 c          214 g          297 t
ORIGIN      
        1 agatatcaat gatgatggct caagaaaaaa agtggagaaa gttggcggag acacccgacc
       61 agaggccgaa gctgctttac gaaaagtttt atcagatatt gacgaaacag gacaatactt
      121 tttaggtacg gatactcgag taaaacaata ccttgatttt tggatggagg aatacgttaa
      181 actaaatcta aaatacaata cctatgaaaa ctaccgattt accatcaaaa atcatataaa
      241 cggttattta ggaaagaaaa aacttacgga tctctcccct gctcttttac aaaatttcat
      301 caatgctgaa tttaaaaagg gttactcgaa gaaaacaatg actattactc actctgtcct
      361 taagaatgcg ctgaatatgg cggtttatcc ttggggatta atcaagcaaa atcctatgct
      421 gtatgtaaaa ataccaaaat atgaagcacg accaacgact aaaaaagatc taaaaatcat
      481 ttctcttgag gactttgatc atatgctaga aattactcct gaaggacatc ctttctatat
      541 tcctttgaat attggatttt atacgggaat gcgcgttggc gaagtttgtg gtctgacgtg
      601 ggatgatgtc gatttttcaa atggaacaat tactgtagag aaacaaatgg taaagaatga
      661 cggcgcatgg gtatatggta cgccaaagac aagcagttcc aatcgaacga tttttattgg
      721 acaaaccttg ctagcaattc tgaaaaaaca taagaaacaa caattagaaa atcgaatgaa
      781 gtatggaaag ctctacattg attcaaatgc agtatgtacg aaggaagacg gtgagctagt
      841 tacgccaagt gtagtgaaat ggaatactag gaggatatcg aatgcactct ccctctcttt
      901 taacttccat tctctcagac atactcatgc tacacttctt ctcgaaaatg gcgcaaaaat
      961 gaaagaaatc tctgaacgat tgggccacag cagaatttca attacgatgg atacttactc
     1021 gcatgtaaca gataagatga gaaatgaaac ggtcgatatc atggaaaatc ttagaaagaa
     1081 ttcttgattt ttgccaccga aaaaa
//
LOCUS       DAYYCY010000300         1101 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.308, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000300 DAYYCY010000000
VERSION     DAYYCY010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1101
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.308"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            23..871
                     /locus_tag="ACHAZB_002813"
     CDS             23..871
                     /locus_tag="ACHAZB_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM8615417.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
BASE COUNT          376 a          194 c          189 g          342 t
ORIGIN      
        1 attatgaaaa tgaggaattc tcatgagcgt ctccataaga ctacaaatca aaacaatgta
       61 caacgacttc agcccaaaag aacaggctat cgccgattat attttagaaa atccaagcaa
      121 agtaagtcat agtcctatta gcaatttagc aagtgaactg ggcattgctg actctacttt
      181 ttttcaattt acaaaaaaac ttggttataa tggatttaaa gattttaaaa tggccatgtt
      241 gatccaagaa aatgatctat ctgctatttc tatccacgaa aatattcaga aaacagatac
      301 tgagctcact atggcgcaaa aggtgttcga gtctagtatt tcaactttaa atgacacaaa
      361 aaagttgtta caacaaaagg atctaaaaaa agcggctact attatcaatg aatctaaacg
      421 tgtctatttt ttcggtgttg gcggttcaga gattgtagca actgacgctt atcataaatt
      481 tttgcgttcc cctatcgcaa cttctcacag cacggattac catattcaat tgatggaagc
      541 ttctttattg acggaaaaag actgtgctgt tttgatttct cacacagggc aatcaaaaga
      601 aacaatccat attgctgaaa ccgtaagaaa aactggagca aaaacaatcg ttattactag
      661 ccaagctaat tcttcccttg ctaaacttgg cgatgtcgtt tttatttcta tttctgaaga
      721 aacagaattc cgatctgaag ctttagcttc ccgtatttct caactaagca ttttggactc
      781 tctatatgtg attttgatgt tttacaacga aaaagaatca cgtcgttcta tcgctaaagt
      841 tcgacatact atttctgaaa tcaaagaata gcaccataac agaaaaagcg aaaaatccat
      901 aaaacaaaaa aagccactac caaatatttg gtagtggctt ttttggttct gcaagggtgg
      961 tacagaacat caatagtcgg tgggttagaa cgtatgtcat cgcatctttg gagaaggttg
     1021 tattggtatt attcgagtta cttgttagat gcgataacaa agttagtata cagtagtaaa
     1081 aaatttatct tttctttttt t
//
LOCUS       DAYYCY010000301         1096 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.309, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000301 DAYYCY010000000
VERSION     DAYYCY010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1096
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.309"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..645)
                     /locus_tag="ACHAZB_002814"
     CDS             complement(<1..645)
                     /locus_tag="ACHAZB_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM8615418.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLD"
     gene            779..1066
                     /locus_tag="ACHAZB_002815"
     CDS             779..1066
                     /locus_tag="ACHAZB_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8615419.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
BASE COUNT          416 a          186 c          185 g          309 t
ORIGIN      
        1 atcaagtggt tcaaatgttt ctgtagtctg ttttgcttcc aattggggca aaacagatcc
       61 aatttccaga tacttcactt tattcaatgt ataagataga atagaacagc taagtaatgt
      121 atacagaaaa tgtgtgagat ccaaataaag caaggagaca actaaaatac caaaagtaat
      181 caagaaaaga cttttgccaa tcttaatatg aaaatatttt tcaatggctt gataaatgat
      241 atctgttccg cctgtgcttc cgccgaatcg aaagacgaga ccgcaaccaa tgccagaaag
      301 aatacctgtc atcaaactat tgatcagtag actatctaca gcaaaatgta tatgaaggct
      361 ctcccataaa tgtaaaaaga aaactagcgc aaatgttccg acgatcgtcc ggagaaaagc
      421 gtgttttcca aaaagaaaca atgagataag caataaaggt aaattaaaaa gcagtgaagt
      481 gacagaagga gcgaatccaa aaagattatg caaaagcaaa gcaataccag taaccccacc
      541 atctgccagc ttagatggaa tattgattcc tgtcacagct aatccgtaga ttcctgcgcc
      601 taaaatgatt ccgcacaatt ctgtaaataa atttaatttt ttcatttcta gcctcttttc
      661 ttttgaaaat tttcagcaac ttctttatta tactgactta tactcttact tcaagaggaa
      721 aatgaaaaaa aattattttt ggctgtttct ttttatttat caaataagga gtggaaaaat
      781 gcgagcagca gacttattga caattcttac aaaatcaccc aaaaaaacag agtatcaagg
      841 agtttttttg gctcaagaac aaaaactggt gcctgtgaca atggtccaga taaataaagc
      901 aaatcaattt atctttttat ttgaacacgg caaagcacca cttgctatga aagacatctt
      961 tctttttttg atgaagaacc gtgagaaagc tgtttattat cagtcaaaag aagaactcat
     1021 tcctctttat ggagtgaaag agatagaaaa taaagtggtg atataagtaa aaataagaaa
     1081 acaagacgtt tatgat
//
LOCUS       DAYYCY010000302         1087 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.310, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000302 DAYYCY010000000
VERSION     DAYYCY010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1087
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.310"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>1087
                     /locus_tag="ACHAZB_002816"
     CDS             <1..>1087
                     /locus_tag="ACHAZB_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-54"
                     /protein_id="HFM8615420.1"
                     /translation="KIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSS
                     IYLGDKLDSGFWIDPEISIDLKKMFLKSLSEIDLIELFQNHISIEESMSQLRDRILKE
                     CAGSSEYNYLESDEFIALRNYVVNKISPIIDSTGLCLLDEFLADISLFIMFIDVIEET
                     SRNFLLSKFRKFRMKNDKQKLLANEIWSSLEVEESKQELLLKWILFLVKKFYVKIPET
                     HCLIVMHGKITASAIARETNKLLNTYVYEAFDMPIEGETSDLINLINNFCKSIDTTNG
                     LILLVDMGSLEQMYEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFD
                     SFQVKVQYYKGLSQKKNVIVSCLSGEGI"
BASE COUNT          394 a          119 c          206 g          368 t
ORIGIN      
        1 aaaatatttt gttcgattga actttttact gatcttttat cgaaaacatt tgaaggaaat
       61 gttggagaat tgaggaatga aatcaagctt ctttgtgcag aaggttattt aggaaataag
      121 agaaagtctt caatttattt aggggacaaa ctagacagcg gtttttggat agatccagaa
      181 attagtatag atttaaaaaa aatgtttctt aaatcacttt ctgaaattga tttgattgaa
      241 ctttttcaaa atcacatttc aattgaagaa agcatgagtc aattaagaga tagaatactt
      301 aaagagtgtg caggttcttc tgagtataat tatttagaaa gtgacgaatt tatagcatta
      361 agaaactatg ttgttaataa aatatcacca attattgatt cgacaggtct atgtttatta
      421 gatgaattct tggcggatat cagtctattt ataatgttta ttgatgtaat tgaagaaaca
      481 tcaagaaatt ttttgctgtc taagttcaga aaatttcgta tgaaaaatga taaacaaaaa
      541 ttattggcaa atgagatttg gagtagttta gaagtcgaag aaagtaaaca agaacttttg
      601 ttgaaatgga tactttttct tgtaaagaag ttctatgtga aaataccaga aacccattgt
      661 ctgatagtta tgcacggtaa aataacagct tctgcgatcg caagggaaac aaataagttg
      721 ctgaatacat atgtttatga ggctttcgat atgccaattg aaggtgagac tagtgactta
      781 attaacctaa tcaacaattt ttgtaaaagt atagatacga cgaatggact cattctgcta
      841 gttgatatgg gatcattgga acaaatgtat gaaaaaattg aatccaatgt aataggtgat
      901 ttggtaattt taaacaatgt ttctacagct ttagcgattg aatgtgggat tcaagtttgc
      961 caaaaaaagc caattagtca tttttaccag atggattttg attcgtttca agttaaagtt
     1021 cagtattata aaggattatc acaaaagaaa aatgtaatag tatcttgtct ctctggtgag
     1081 gggattt
//
LOCUS       DAYYCY010000303         1084 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.311, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000303 DAYYCY010000000
VERSION     DAYYCY010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1084
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.311"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            146..928
                     /locus_tag="ACHAZB_002817"
     CDS             146..928
                     /locus_tag="ACHAZB_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HFM8615421.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
BASE COUNT          379 a          152 c          220 g          333 t
ORIGIN      
        1 ctgtagacaa attgtgaaag gatgtactta aacgctaacg gtcagcttta ttgaacagtc
       61 atttaggtat atgtccaatc tagggtaagt aaattgagta tcaatataaa ctttatatga
      121 acataatcag ttacgaggtg aaatcatgag caatttgatt aacggaaaaa taccaaatca
      181 agcgattcaa acattaaaaa tcgtaaaaga tttatttgga agttcaatag ttggagtata
      241 tctatttggt tcagcagtaa atggtggttt acgcattaac agcgatgtag atgttctagt
      301 cgtcgtgaat catagtttac ctcaattaac tcgaaaaaaa ctaacagaaa gactaatgac
      361 tatatcagga aagattggaa atacggattc tgttagacca cttgaagtta cggttataaa
      421 taggagtgaa gttgtccctt ggcaatatcc tccaaaaaga gaatttatat acggtgagtg
      481 gctcaggggt gaatttgaga atggacaaat tcaggaacca agctatgatc ctgatttggc
      541 tattgtttta gcacaagcaa gaaagaatag tatttctcta tttggtcctg attcttcaag
      601 tatacttgtc tccgtacctt tgacagatat tcgaagagca attaaggatt ctttgccaga
      661 actaattgag gggataaaag gtgatgagcg taatgtaatt ttaaccctag ctcgaatgtg
      721 gcaaacagtg actactggtg aaattacctc gaaagatgtc gctgcagaat gggctatacc
      781 tcttttacct aaagagcatg taactttact ggatatagct agaaaaggct atcggggaga
      841 gtgtgatgat aagtgggaag gactatattc aaaggtgaaa gcactcgtta agtatatgaa
      901 aaattctata gaaacttctc tcaattaggc taattttatt gcaataacag gtgcttactt
      961 ttaaaactac tgatttattg ataaatattg aacaattttt gggaagaata aagcgtcctc
     1021 ttgtgaaatt agagaacgct ttattacttt aatttagtga aacaatttgt aactattgaa
     1081 aata
//
LOCUS       DAYYCY010000304         1078 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.312, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000304 DAYYCY010000000
VERSION     DAYYCY010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1078
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.312"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..231)
                     /locus_tag="ACHAZB_002818"
     CDS             complement(<1..231)
                     /locus_tag="ACHAZB_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6037 family protein"
                     /protein_id="HFM8615422.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLY"
     gene            complement(287..787)
                     /locus_tag="ACHAZB_002819"
     CDS             complement(287..787)
                     /locus_tag="ACHAZB_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000425404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFM8615423.1"
                     /translation="MEMKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEE
                     YAIHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENK
                     EDIHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHG
                     VFEYCQ"
     gene            complement(848..>1078)
                     /locus_tag="ACHAZB_002820"
     CDS             complement(848..>1078)
                     /locus_tag="ACHAZB_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HFM8615424.1"
                     /translation="SRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLNEARNRHSLA
                     HPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
BASE COUNT          325 a          215 c          140 g          398 t
ORIGIN      
        1 atataatttt accgaattag catagacagc tagcatatct gaaaaatcgt tttcctttaa
       61 aaattctagt tttaacagag catattctgg aactttttcc tctttttcaa atagcttaac
      121 aagtacaata taattttgct gtttgtaacg aaagtaaaag gaatctatca cccagccttt
      181 atctttcatg tctagtttta atagtttaag attttcaaat acatttacca taatttatac
      241 ctctccgtgc tatttattaa tttaattata acacggattg ttaaacttac tgacaatatt
      301 caaatacccc atgagaggta actaggtaat taccctctat ttccatatca cgtcccaaag
      361 attggtagtc aatataattt tgtaaattgg aagggacttc accaagctgt cctgtttctt
      421 ccacgtaata acgggcgaca tcttccatcg aatcacaatc actataacaa tgaatatcct
      481 ctttgttttc cagtaattct tccaagctac taaaccacat gccttgaatt tctttgagtt
      541 cattataaat aggtgtacct tcgatttctt gaatggcttc acatagacgg ttgatttcac
      601 taatcggcgt gtattcgtcc acatcaaaag gaagctcaaa atcatgtatt gcatattctt
      661 cgtagtcctc atttaaaccg attctttctg ccatttcttc ctcatcaatt ggtggagtaa
      721 accatgctcc gactaattca ccttcattat accgtcctaa atttgccaca tagactttca
      781 tttccaattc ttatcacatc ctttttataa aaatagtcat aaaaaaagta gcaatcagtt
      841 aatgacgcta cttatcaagg tatccgctaa tataatctgt aactgtactt gaaataccta
      901 ttattagttt tgcttctctc tttccaataa tatttgtatt agggtgtgca agtgaatgcc
      961 tatttcttgc ttcattaaga gagctaatca ttccatttga tgaccttatc gttgtcttca
     1021 ctatatcagc aagttcagta ggttgaattt ctttttcaat taattgttgt atcctact
//
LOCUS       DAYYCY010000305         1077 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.313, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000305 DAYYCY010000000
VERSION     DAYYCY010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1077
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.313"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..846
                     /locus_tag="ACHAZB_002821"
     CDS             1..846
                     /locus_tag="ACHAZB_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8615425.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
BASE COUNT          409 a          137 c          183 g          348 t
ORIGIN      
        1 atggatgaaa tgaaactaat tggtatagat ttagacggta ctcttttgaa ttctgaccag
       61 agcattagtc aaaaaaatgc aagaacaatg aaacatttac cggaagactg ttttccattc
      121 atttgttctg gaagagaagt agaagacatt aataatattc tcaaaaaatc tggtttaagg
      181 ataccagcag tagggttaaa tggttctgtt ggatttgatg gagaaaaaaa gatatttgag
      241 tcttccttcg ataaaaatga tgtaaaaaaa attgattctg tcctatcgaa atttccaacc
      301 aaaatatata caaatctcgg aagttatgaa tctcagaatt ataaaaataa aatgaaaaaa
      361 atttttcacg aggtagggag tgaattttct atcaaagagc taaactatga gcttgagtat
      421 gaaaaatcta tttgttcaat cccttataat aatattgagg aaattataga aaaggatgct
      481 atcagcattt acaaattttt tatttttatt cctaatcgtc aattgaaagc taatatattt
      541 aaccatttga aaaagatgct aaatgtgtct gttactgaat cggcacctgt aaatattgaa
      601 atcgtacctc aaaatgtatc taaaggtgtg gtgtttgatc gtttggaatc aatctacaat
      661 ttaaagaaac ctttgagaat cgcgatcgga gatagtttaa atgacttatc tatgtttgaa
      721 agtgcagata ttagttttgc aatgggaaat agtcatcaag aaatcatgaa aaaagctgat
      781 tatgtcactg tttcaaatga taaagacgga gtatctcaag catttgaaag aattcttacc
      841 atataaacta attaaggtag tatagaacat agaattctaa atacggaaaa atggagcata
      901 aattccagta caaatagtac ttcttcagtg tcaaacgaag cgaatataac atagatattt
      961 aggattgtga caaaaatcat gtcacaatct ttttttcgaa taaacttatt ttttgaagtc
     1021 ggccatttat cgtaatttct ttgcataata cgaacaaatt ttcggttatt gatgaaa
//
LOCUS       DAYYCY010000306         1073 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.314, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000306 DAYYCY010000000
VERSION     DAYYCY010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.314"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..723
                     /locus_tag="ACHAZB_002822"
     CDS             <1..723
                     /locus_tag="ACHAZB_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFM8615426.1"
                     /translation="FLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFSFGT
                     NYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIWKAA
                     LKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLVSSG
                     IIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFNKRD
                     VISIVFWYYLGSISYLYLKKEN"
     gene            725..>1073
                     /locus_tag="ACHAZB_002823"
     CDS             725..>1073
                     /locus_tag="ACHAZB_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8615427.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSFKRKINLLTNHNQFFSDDLSVYDEIFIQGENYFTK
                     ILFSNVYKKNPDVK"
BASE COUNT          362 a          129 c          179 g          403 t
ORIGIN      
        1 tttttagtat tatttctttt gttgtttgcc aattcaaaat ttagaaagag cattagagaa
       61 gtactacctg taaaaatcaa atggatggta agcatattat tagttctatt ggtatatttt
      121 tcttttggta ctaattatgc taaggatttt ttagcaacaa ttcctgtatc gattgataga
      181 aaagtagaag ttatagagtc aaatgaatca gataaaactc cacttccaga aaaagaagta
      241 atattacagc attctagtga taactccgaa atttcagctg gtcgttttac gatttggaaa
      301 gctgcgctta aagttataag accaactttg ttatttggtt atggagagac cgatttctat
      361 gggaatagcg atgcaaaatt tttgaaaaaa gtagatcttg atactacaga tgtgaatgaa
      421 ctaaaaagag cacatggaaa catgcataat ggatttcttc aagtacttgt atcaagtggt
      481 ataattgctt ttatatgtat ctatgctttc tatgtgttaa atatctttag tttactcaaa
      541 acatttttta gaagtaaagt gaattatgcg tttcttggta ttgttttaat ttttatacta
      601 tctattttta tagatgaaat ggtggaggct catgtacttt tcaataagcg cgatgtcatt
      661 tcaattgttt tctggtatta tttgggaagc atttcttact tgtatttaaa aaaggagaat
      721 taatatgaag acagctttca ttgcttggac tcctttacat ttgattaata tattaaatgt
      781 ttatcaaaat ttttaccgag aatcaaaagg agatttattt atttattctg aatttgaaaa
      841 cgctagaaga tatagtgagc gtataaaaga attagggcta tttaatgatg ttgtttttgt
      901 gaatcaccgg aaaatcggtt cttcctttaa acgtaaaatt aacttgttaa ctaaccataa
      961 tcaatttttc tcggatgatt tatccgttta tgatgaaatt tttatacagg gggaaaatta
     1021 tttcacaaaa atacttttct ccaatgttta caaaaaaaat ccagatgtaa aac
//
LOCUS       DAYYCY010000307         1064 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.315, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000307 DAYYCY010000000
VERSION     DAYYCY010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1064
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.315"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..453
                     /locus_tag="ACHAZB_002824"
     CDS             <1..453
                     /locus_tag="ACHAZB_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HFM8615428.1"
                     /translation="LKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTTLFVSVIY
                     FALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGLLPKFMKV
                     ADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            462..689
                     /locus_tag="ACHAZB_002825"
     CDS             462..689
                     /locus_tag="ACHAZB_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8615429.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT          329 a          178 c          185 g          372 t
ORIGIN      
        1 ttaaaatttg tcgttttagg tataagtgcc gtatttattt tgttcggcgt acttttttat
       61 actcagatcg ctacaagcga aaaaggattc aattttgatt ggcagaccac attatttgtt
      121 tctgtcattt actttgccct tctcctaggg atcgttattg cttatttctt attgaaacta
      181 ttgaaaaaaa tcgaagataa ccaagctttt tccactcaaa gcttaaacat actaaaaaaa
      241 atcgaacgaa ccattttgtt tattggtttc gccttttttg gcttacttcc taaatttatg
      301 aaagtagcgg atttagacga tgctccaggt ttgatgttgg taggaatcgc tgtcgtattc
      361 attccttttg cagtatacac attaacaagc gtgctggaaa aactgcttga acatgcgatt
      421 ttacttaaga cagatcatga tttgacagtg tgaggtgcga catggcaatt atcgttaatt
      481 tagatgtagt attagctcaa aaaaaaatga gtgtgactga attgagtgag aaagtgggca
      541 ttacgatggc aaacatctct attctaaaaa atggaaaagc aaaagccatc cgtttttcca
      601 ctctcgaaaa aatctgccaa gttttggagt gtcaaccagg tgatttgttg acctataaaa
      661 atgactcgaa tactatacca gaagaataaa ataaagtaga acaaacagta aataaaaaat
      721 aatctgaatg atagatgtgt gagactttta gaagttattt acgtctatta ttcagatttt
      781 ttatttcgat tgaataccgt tttatctata aataagatat gtactgactc atacaaaggg
      841 ggccttttct tctccatgta ctatcacttt tcctgataca actggtttcc tgttacgttg
      901 tagttggatc tgcttttagt ataatacttt attcattcca ccagactgat agcagtttga
      961 gcagtctaaa taatcatgta gtattacagg ccattctgtt tcataccttg aagtgcttaa
     1021 aacttcagct aatggcccat tgtgttaact cttactccat tctt
//
LOCUS       DAYYCY010000308         1060 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.316, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000308 DAYYCY010000000
VERSION     DAYYCY010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1060
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.316"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(38..994)
                     /locus_tag="ACHAZB_002826"
     CDS             complement(38..994)
                     /locus_tag="ACHAZB_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8615430.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          344 a          176 c          160 g          380 t
ORIGIN      
        1 tttagaaaaa gcagtcggac tttctaaaag aagttcacta ttcgatcctg tcgatgattt
       61 cttcaggaaa atcgtctatt tgcttgtcaa ttttgctgtc tttgtatttc tcttcttgga
      121 tagtcaacaa aatctttgta agattccccc agtctactgc tcttggcgga tcgagccaaa
      181 ccactacttg agatgcactt tcttcatata gattattcct attcgtgatt ataacatccg
      241 ggaactcagg ctttcctgcc tctatcactt ccgtgttcag attaatgtac aagcttaaat
      301 ttttcttgat aaattgatta taggcagcac cataagaaaa atcgatgatg atctttactg
      361 gttcagaaat cagttgaatg ggtatgtgat tgatcaataa taataaataa ctgaaaaaaa
      421 gaaaaaattt atcttgaagc aactctttgc gttttgacgt tagatcattt atatactcta
      481 tcaaatagca ataaaatgct ggatagtttt ctctaaaaaa agaaacatca agtggaaatt
      541 catcagcttt attaatagtg taatgcaaaa gtttaaaatg tattttggtt aactctcttc
      601 ggatcaattg gctcgtctct tctcgcaaca gattaaactg attttcaaaa ctttcaatga
      661 atcgttcatt caaccattcg attgtttcat cttcattttt tctcaagtat ttttgatcca
      721 atttatcatg aagataaagc caagagagtg tcttattgat aaaaataacg gatttttctc
      781 ctaatactcc ttttaagtgg taaaatccta tatccgaaaa aaatagcgac tgattgctag
      841 caatatctaa agaatcagct tgttcagcca acaggttgat tgctaaaaag tggaataatt
      901 tctttttttc ataagcagac agaggataat actgttttaa atcttttaac attttttctg
      961 tttttataat tataggtagc gtatacaatt ccacaaaact tatctctgtt gagttaaaaa
     1021 gattatataa aaaaagccga atgtttttag gattccctgt
//
LOCUS       DAYYCY010000309         1050 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.317, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000309 DAYYCY010000000
VERSION     DAYYCY010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1050
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.317"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            13..636
                     /locus_tag="ACHAZB_002827"
     CDS             13..636
                     /locus_tag="ACHAZB_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8615431.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
BASE COUNT          362 a          161 c          214 g          313 t
ORIGIN      
        1 gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat cgagagtaca agaaatgaat
       61 gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt attttcgacg tcttgttttt
      121 ctgctcatct tatcattgct gttaacagtt gcaatgtttt tattgccgga aaaaatccga
      181 gaagagcaag taaatagtct cgtttattcg tccataccaa ccagcaaatt gaaacctttt
      241 gattacagca aagctgatca gaaaatcaaa caaagccggg cgatatcggt gatgttcagc
      301 aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt ttaatgatcc tgagcttatg
      361 gaagaattga atcgaccgat cttttattat ccgatcgttt atgatgtaca tgaactggaa
      421 caaaaatata acatacgaac ggatgaagtg acatttattt tctttgatgg aggaaaagaa
      481 gcaaatcgga taactgcggg taagggagga ataactgatt ttgataagga gttgatccct
      541 gaactgaatc gacttccgtt agccaatatc aaacaattag aagaagaatt ggatagaacg
      601 ggagtaactt cccaaaattc cgagattgct aattgagaca ttggcgcgat gaaacggtgg
      661 gtctatatag cgctattttt aattatcaga acaagtgatg tttgatcttc aaaataacaa
      721 ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt cgattagact taaatcaatt
      781 cttaaggaaa gggtgacctg taatgaactt atcaaaacgt atttcagcag aaaacaaaaa
      841 aaattggcgc agctggcgta gataagttgt attaatggtg agactttccc atagatacaa
      901 ctttttagag tgcatttcta aagcttgtat ctatgctaga catgaacatt gactgccgca
      961 tagattagaa tctatgcggt tttttgtgta aagacaatga ccgctttggc cattgtcttt
     1021 ttttgtagac tggatacttg cctgattata
//
LOCUS       DAYYCY010000310         1033 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.318, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000310 DAYYCY010000000
VERSION     DAYYCY010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1033
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.318"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1024)
                     /locus_tag="ACHAZB_002828"
     CDS             complement(<1..1024)
                     /locus_tag="ACHAZB_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFM8615432.1"
                     /translation="MYLLNITKLWQDVYGLEKYIFLYYISYVWNQFLIQFAKGLEKVK
                     AMGIAGIISTIVTIIANIFFLLILKWGLIGFFIANILSQIISATYLSVLVRIWEYIDF
                     EAKDPQLKREMLNYSIPLIATVVGWWINSTADRYVVSFIVGVSANGLLSVAYKIPQII
                     NTLQGIFIQAWQISAIKEYGDNETAKFYGNTFVMINLLMCITCSSLIILTRPFSHILY
                     AKDFYEAWKYVPFLLIANVLNCASGLLGPILSAKKDSKSMMWSAIIGALVNVIMSVVL
                     VYKIGVQGATIATVICSYVIYVVRKRAVGKDIFIKHYHIVVESWGLLFVQAIIEIYFK
                     NYVIEII"
BASE COUNT          382 a          194 c          121 g          336 t
ORIGIN      
        1 ttataatttc tattacataa tttttaaaat atatttctat gattgcctga acaaacaata
       61 atccccaaga ttccacaaca atatgataat gctttataaa aatatctttt cccacagctc
      121 gtttacgaac aacatatatt acatatgaac atataacagt ggctattgtg gcaccttgaa
      181 cccctatctt ataaacaagg actacactca taatcacatt aactaatgcc cctatgattg
      241 cagaccacat catggattta gaatcttttt ttgctgataa gataggtcct aacagacctg
      301 atgcacaatt taaaacatta gctattaata aaaatggaac atatttccac gcttcataaa
      361 agtctttcgc ataaagaata tgtgaaaacg gtctagttaa tatgataaga cttgaacagg
      421 ttatgcacat taacaaatta atcataacaa atgtatttcc ataaaatttt gcagtctcat
      481 tatctccata ttctttaatt gcagaaattt gccaggcctg tataaatatt ccctgcaaag
      541 tattaattat ttgaggtatt ttatatgcca cagacaataa gccattcgcc gagacaccta
      601 ctataaagga aaccacatac ctatcagctg tactattaat ccaccatcca acaacagttg
      661 caattaatgg aatcgagtaa tttagcattt ctctctttag ctgaggatct ttagcctcaa
      721 aatcgatata ttcccatatt ctcactaata ctgacagata agtagctgaa ataatctgtg
      781 ataaaatatt tgcaatgaaa aaaccaatta atccccattt cagaataagc aagaagaaaa
      841 tattggcaat tattgttaca attgtgctga taatacctgc tatgcccata gctttaactt
      901 tttctaaccc tttagcaaat tgtattagaa attgattcca tacataggat atataataca
      961 aaaaaatata tttttctaat ccataaacat cttgccataa ttttgtaata tttaacaaat
     1021 acattacgga tcc
//
LOCUS       DAYYCY010000311         1023 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.319, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000311 DAYYCY010000000
VERSION     DAYYCY010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.319"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            142..>1023
                     /locus_tag="ACHAZB_002829"
     CDS             142..>1023
                     /locus_tag="ACHAZB_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFM8615433.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAF"
BASE COUNT          364 a          196 c          148 g          315 t
ORIGIN      
        1 caagtatact gtatatactt tacacaactg tttcatttta tttaaattta tgcagttata
       61 ttctatatct tacaaacaca ttccatgtat tccaattccc tatccgttat aataaaagaa
      121 acaggcaaca ggaggaaaat catgatcaaa tattttacat tagataatga gaaactgcgt
      181 aataccggaa aggaaactga ccaaacgatc tggtattgtg tagaacgtcc taatcaagaa
      241 gagattcatc agctgacaca tcaatttgaa attccgtcag attacatcac ttccatattg
      301 gacgatgcag aaaattcccg tacagaagaa ttcaatcaat caaagttgac aaagccagcg
      361 ttattattgt tacaatatcc ttttgctaaa acaagtccta gcggttattt tcaagtggat
      421 acatacccgc tctctattat tttgacgccc aaaaagaagc ttatcacaat tacgaatcat
      481 gaaccactgt ttttaaagaa agtctttagc cagacctttc caaaaagtga tatttcgcct
      541 aatctcaata tcttctttca attcatttgg caggtcacac tctcttacaa cagttattta
      601 gctacgcttc aatctcaatg cgatactttg gaagccaaac ttcaagtatc cactgaaaac
      661 aaacaacttt atcaaatcat ggatatccaa aaaagcctcg tttatttcag agaagcgact
      721 tctgctaatt tagatgcatt gaaagaacta gcgcaaaata aacttttcca agaaaatcat
      781 gctttgaaaa atcatttacg tgatgtattg gtggaaacag agcaggcaat gactacttct
      841 cgcattcaat taaaactagt agaacaaatg aatcagactt tttcagcaat cgtatcgaat
      901 aatctgaata atattatgaa aattttaact tctttaacga ttattttaac tataccgaca
      961 atcgtcggca gtatctatgg aatgaacatt aagttaccta ttgctgaaag ggatgatgcc
     1021 ttt
//
LOCUS       DAYYCY010000312         1015 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.320, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000312 DAYYCY010000000
VERSION     DAYYCY010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1015
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.320"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..962)
                     /locus_tag="ACHAZB_002830"
     CDS             complement(<1..962)
                     /locus_tag="ACHAZB_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8615434.1"
                     /translation="MERKFGLRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     TVGTLFLVARFIDAVTDITMGRIVDGSKPSKTGKFKPWILRMSGPVALASFLMYQTSM
                     ASASMSIKVIYMYVTYILWGSIFYTSINIPYGSMASAITSVAEERTALSTFRSIGATL
                     AGMFIGVLTPIFIYTRDSSGAQIVRGGSTFTIVAGVFSVCAVICYVICYKLTVERVKS
                     DESQLKVSLLKSLKGLAKNRALLSIIAAAIFLLLAQLLTMSMNNYVFPDYYNNAQGIV
                     LMNFINPILMLVLVSPVSLIFSKKYGKKELASVGMFFSAIVYLL"
BASE COUNT          364 a          201 c          129 g          321 t
ORIGIN      
        1 aaaagtaaat atacaatagc agagaagaac atccctactg acgccaattc ttttttaccg
       61 tattttttac tgaaaattaa acttacaggt gaaacaagta ccaacatcaa aataggattg
      121 ataaagttca ttaaaactat tccctgtgca ttattataat aatctggaaa tacataattg
      181 ttcatactca ttgttaaaag ctgagcaagc aacaaaaaaa tagctgcagc tattatagaa
      241 agcaacgctc tatttttagc taatcctttt aaagatttca acaatgaaac ttttaactgc
      301 gattcatcac ttttaacccg ttctacagtt aatttataac atattacgta acaaataact
      361 gcacacactg aaaatactcc tgccacaatt gtaaaagttg atccaccacg tacaatttga
      421 gctccagaac tatctcttgt ataaataaaa attggtgtta acacgccaat gaacatacct
      481 gctaaggtag ctcctatact acgaaatgtt gataacgctg tcctttcttc tgcaacagaa
      541 gtgatagctg atgccattga accgtaagga atattaatag atgtataaaa aatacttccc
      601 catagtatat atgttacata catatatata actttaatac tcatagaagc tgatgccatt
      661 gatgtttgat acattaaaaa acttgctaaa gcgactggac cactcatccg taaaatccac
      721 ggtttaaatt tgccagtttt agatggtttt gatccatcca caatacgccc cattgttatg
      781 tctgtaactg catcaataaa tcttgctact aaaaataatg ttcctacggt agctccgctt
      841 atccccaata cttttgtata aaaaatcatt aaaaaagaac tagcaaaaat gaaagtgaaa
      901 tcattcccaa aatctccaaa catatatcct attttgtctc ttagaccaaa ttttctttcc
      961 atataatttg cgctccttcc tattgttaag cgttttcatt ctgttgaact gccaa
//
LOCUS       DAYYCY010000313         1010 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.321, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000313 DAYYCY010000000
VERSION     DAYYCY010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1010
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.321"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..848
                     /locus_tag="ACHAZB_002831"
     CDS             <1..848
                     /locus_tag="ACHAZB_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFM8615435.1"
                     /translation="EIADLIEKMYGHHYTPQTMSNITKSFTEEVTAFKGRELHDRYAA
                     IYMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQER
                     GLKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVFRNISHKVRVNNRKEVCDDFKM
                     VYQASSKEVALEARGAFAKKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLI
                     ESFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLS
                     QPMEN"
BASE COUNT          329 a          195 c          202 g          284 t
ORIGIN      
        1 ctgaaattgc tgatttgatc gaaaaaatgt acggtcatca ctatactcca caaaccatgt
       61 ccaatataac aaaatcattt acagaagagg taacggcgtt taaagggcga gagcttcatg
      121 accgttatgc tgctatttat atggacgcaa cgtatattcc gttaaagcgg aaaaccgtcg
      181 ccaaggaagc tattcatatc gcagttggca ttcgcccgga cggatcaaag gaagtattga
      241 gctatgcgat tgcaccgact gaatccatca cgatttggga ggaaatttta ttagaccttc
      301 aagagcgcgg tttgaaaaat gtcctcctgt tcatcacgga tggcttaaag gggatggtag
      361 gagcgattag tcggttctat cccaaagctc gttttcaaca ttgttgtgta cacgtttttc
      421 gtaatatcag tcacaaagtg cgtgtcaata atcgtaagga agtctgtgat gattttaaaa
      481 tggtgtatca agcctcatct aaagaggtgg cgttagaagc acgtggtgct tttgcgaaaa
      541 aatggaaaac cagctatcca aaagtggttg aatcgattct ttcgaacgat cacttgctca
      601 ctttctatga tttccccttg gccatacgca agagtattta ttctacgaac ttgattgaat
      661 cctttaataa gcaaatcaag aaatacagcc accgcaagga acagttccaa aacgaagagt
      721 cgatggaacg tttcttagtc tcgtcttttg atacttacaa ccaaaaattc ctaggtcgca
      781 gtcataaagg tttccaacag gccgaaggcg agctcgaaca aatgctgagt caaccgatgg
      841 agaattagaa acaatctaca gtagggaaga accatttaca caaaattatt gacgctccca
      901 agctaaaact cctattaatc gatctactaa aaataaataa aaaaattctt ttcaattttt
      961 atttatggta tgacataaat aaagtaaagt tattcttcta ggttaatctt
//
LOCUS       DAYYCY010000314         1010 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.322, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000314 DAYYCY010000000
VERSION     DAYYCY010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1010
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.322"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(62..286)
                     /locus_tag="ACHAZB_002832"
                     /pseudo
     CDS             complement(62..286)
                     /locus_tag="ACHAZB_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877485.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(840..926)
                     /locus_tag="ACHAZB_002833"
                     /pseudo
     CDS             complement(840..926)
                     /locus_tag="ACHAZB_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
BASE COUNT          346 a          175 c          151 g          338 t
ORIGIN      
        1 taggtatttt tattatatat agatttaaca tatgtcggtc ggttgattga tatagctaaa
       61 gttataactc aaccgaagcc gattcatcta cacgaataaa gttgcgagtg ttctcggcta
      121 attcataaaa attgttatgt gttccatata gcaatactgg aataacttta ttttcttgaa
      181 tctcataagc aactctatac acggtgaccg catacttaaa tccattagac caattacctg
      241 ataaacctcc agttttttgc tcttacttaa atggatcaat ggcaatttca tcacaaacct
      301 atagaggatg agtgacttct gcctaattac gttaaaaaat attgaaagta actctatgta
      361 gcaaaaaata tctagaattt catttcctca aaactgatcg atattgtatc taaaaatata
      421 tactacctaa aataggtagt atatattaaa atatgtgctt ttgttagaaa aatcaataca
      481 taaattatca tatctgtata tgtcatttag gtatttcagt tatgttataa tagttttgct
      541 gtctgctacc ccggcctaat ctggcaaggg gaggaggaga tataatgaaa tacctttttg
      601 attttgtttt tgcaatcaag ccacttgcag atgtagtgtg cagttacctc tgcaaatggc
      661 ttgattgcaa aattgccaaa aaagacagta agcctacccg ttaattctat aatatagaaa
      721 cgtaaaaaaa accccatcag cctgcacgct gatggggttt ggtgatataa tgaaatacct
      781 ctttgttact cataattgta ccataattag tcacgaatgc aaccaataga aaaaactact
      841 tagaacggta gatcatcgct aataacaatg cattgtccac caaatggatt ttgtgaagca
      901 ttaaatacat tgttattaat tgtcgctgct tttaactagg cctctcccct tctattataa
      961 aagaagatat atatcatttc ttaattacct actaccttaa accaaaattt
//
LOCUS       DAYYCY010000315          995 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.323, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000315 DAYYCY010000000
VERSION     DAYYCY010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.323"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            417..563
                     /locus_tag="ACHAZB_002834"
                     /pseudo
     CDS             417..563
                     /locus_tag="ACHAZB_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332517.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamate
                     endopeptidase"
     gene            complement(651..944)
                     /locus_tag="ACHAZB_002835"
     CDS             complement(651..944)
                     /locus_tag="ACHAZB_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HFM8615436.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
BASE COUNT          307 a          166 c          151 g          371 t
ORIGIN      
        1 aaaaaagata aaacttgatt aattgaagta ttgcttaaat ttggaagcta tgagggaaga
       61 ataaaagagt taggacggga ttatctacta tttttctttg ttttaaatat ttgttatctt
      121 tgaacataaa agagaaatgt gtcgactgca gtgttgtgtc cctcatcacg aataaacggt
      181 gttcaattag ctgatcttgt attcacttaa aaaatggtaa agtacgagtt gctattcccc
      241 attttcaatt ttctaacgct caggacaaaa tactgttttc acaacctcat tggaataaaa
      301 gcatatatat tgtttcaaga acgaaatctt gttatctttt taaagggcat ctgtccaaaa
      361 tctaatttta ggaggtacgt tttggtattt attcgtacaa ataaaagaga attatcttta
      421 ttcatgtaac gcatccaatt gattttttca tgtacgctcc tggaacgatt ttactgacga
      481 ttgcttattt aactgcgaat cgctcattag cttttatcat agctatccat attttgaata
      541 acgttcttgg ctttgtatta tagatacaga tgacaaaaaa agttcaagta tgcggtgtag
      601 aaccaatatc gcatacttga acttttttca atgagtataa attaaaactt ctaattttca
      661 attcccatca tctgatatgc ccgaccgtct gaacttcttt tgaaaaagcc aaatgtgatt
      721 agataacgtc tttgttcgac ataatcgcca ctagaaaata cgttcagcag tatcgaatta
      781 acctctgttt ctgtataagt cactccatgg tcaaagcgtt tcaagacttc tttcaatagg
      841 atgtatttct tttttgttga acttggtact tgttttactt ttccatcatt catgaatgtg
      901 tctaaaactt tcatcttttc ttgctccgta taagtataaa ccatcttctt tctccttttg
      961 attctaagaa tactccattt atgttagaat attct
//
LOCUS       DAYYCY010000316          995 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.324, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000316 DAYYCY010000000
VERSION     DAYYCY010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.324"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(26..913)
                     /locus_tag="ACHAZB_002836"
     CDS             complement(26..913)
                     /locus_tag="ACHAZB_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFM8615437.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT          288 a          207 c          179 g          321 t
ORIGIN      
        1 taatttcttt gcctttttat ctaaatcaaa cgtttagttc ttgaccaact aataaagaat
       61 aactggacat agacatgcca tttttcgcca ttagatcatc aacagacatg ccaaattttt
      121 gtgcaatatc ccatagcgta tcacctgaca cgactgtata tgtctgacca ttatttgtat
      181 ttacatctgt tgttgaagat gatgacgttg aagtcgtcgt agtagtcgtt gttgttgtac
      241 ttgtggttgt tgtagccgaa cctgttgatg gcgtatcaaa acgggttaaa ttataagtat
      301 taatcaatga gttcaatttg gccccataat ttggatcggt agcataacga cctgtcaagt
      361 acgctgttgc atcataaaaa cttgttgtat tgcttttcca cacgccagca taatgataat
      421 cgcccgtgga aaaactggtt gaacgaagaa cattcgcatg gtcttggaag gattcccaat
      481 aagaagaata ttttcggaat ggctcattca tcgttaccca ttgtccgttt acatattctt
      541 tggttgccat ataaacgacc tgtccgttat agcttccttt cacaccgaat agattataat
      601 ttggcgcact agctaatccg gaagtgccat atgcactttc caacaacgct tgagcgatca
      661 tgactgaagc atacaagtca ttacttgcag caacttctga cgcagaccag ccgatttgat
      721 cgataaacgc ttgaggatca gcgactgttt gagatccttg ttcatctgca tgaacatgtt
      781 gcggtaccgc tgtaaccatc atcggcccaa ctaaaagact cgttccaatt aaaggagcaa
      841 tcgctttttt attgatcacc atttttcttt taggtctatg gtgccgggca ctgcgggtct
      901 tcagctcttg cattcaaatt cctcctcaaa ataaaaaaac ttaataaaaa gttctcttct
      961 taaaacaaac ttaattaatt aaattatact tttaa
//
LOCUS       DAYYCY010000317          984 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.325, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000317 DAYYCY010000000
VERSION     DAYYCY010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..984
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.325"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            14..>984
                     /locus_tag="ACHAZB_002837"
     CDS             14..>984
                     /locus_tag="ACHAZB_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8615438.1"
                     /translation="MRWYMNLKKIFSVKIFIVYAFVSLLYLFPAFKNMEIFGFYDTAF
                     HINRALSLESIFSSPINFETFRSYGMQVNNFYPWLTLYPLFLLIKFTNLAIGYNLFLY
                     IVTLITLFICHYVMYEITKKHVTSSFFAIIYTTSSFRSVEIFLRGAMGELLAMSILPL
                     ILLGFIKLYDSKKESWVMLAISMTLLIYTHVLSVAMTMVMIIIALIIQFYRKKKIEIF
                     LIIKLIKAAIVTLLLSLAFIGPMIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDS
                     ELLSFGIGLTLLICLIILTFNFKKLGILSKITYIMGIISILLCTKIFP"
BASE COUNT          323 a          128 c          136 g          397 t
ORIGIN      
        1 aatataaaaa aacatgaggt ggtatatgaa tttgaaaaaa attttttctg taaaaatatt
       61 tatagtttat gcatttgtta gtttattata tttatttcca gcatttaaaa acatggaaat
      121 atttggcttt tatgatacgg catttcacat taatcgtgcg ttaagtttag aatctatttt
      181 tagctcccct attaattttg aaacgtttag atcatatggc atgcaagtaa ataattttta
      241 cccatggtta actctatatc ctttattttt attgatcaag tttactaatt tagcaatagg
      301 ctataacctt tttctttata ttgttacgct cattactcta tttatatgtc attatgtaat
      361 gtatgaaatt accaaaaaac atgttactag ctcctttttc gctattatat atacaacatc
      421 ttcttttagg agtgtagaaa tatttcttcg aggggcaatg ggggaattac tcgcaatgtc
      481 aatacttcct cttattttac tcgggtttat aaagctttac gactcaaaaa aagaatcttg
      541 ggttatgttg gcgattagca tgactttatt aatttacact catgttcttt ctgtagctat
      601 gactatggta atgattataa ttgctttaat tattcaattt tatcggaaaa agaaaataga
      661 aatcttctta attattaaat taataaaagc tgctatagtt actttacttc tttcactagc
      721 gtttataggt cctatgattg agcagacatt gtatcaagat ctgaatagac cttatgtgga
      781 tatattacaa aaaagagcaa tttatctggg taaagagttt ttgattggaa gtatagattc
      841 agaattacta tcatttggta ttggcttaac tttgcttatt tgtttaatta tactaacatt
      901 taactttaaa aaattaggaa tcttatctaa aatcacttat ataatgggta taatatctat
      961 tctactttgt acaaaaatat ttcc
//
LOCUS       DAYYCY010000318          982 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.326, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000318 DAYYCY010000000
VERSION     DAYYCY010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..982
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.326"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            349..982
                     /locus_tag="ACHAZB_002838"
                     /pseudo
     CDS             349..982
                     /locus_tag="ACHAZB_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          333 a          181 c          179 g          289 t
ORIGIN      
        1 atgtagttat atatttaaat ttttaccaga aagaaatgaa caccttcaat tatctaaatc
       61 tcgaaaatgg ttagatgtaa tcatcaggaa aaacaatctt aaacgaatta ctattcatgg
      121 tcttagacac acacatgcta gcttgctttt agcaagctag tgcaaatatt aaagatgtgc
      181 aagagcgttt aggccactct tctattcaaa tcattatgga tctatatatt catattacag
      241 acaaaagaaa agaagaaacc gtagcgcaat tcgcaaaata taggctctat aatttatggg
      301 actgatgaat caaaattgat tcttcagtcc cattgttatt ttaggtatat gattcgtaca
      361 ttgaaagagt tgaaaaacta tcttccgaag ccgtcaaaaa ggaccctgcc aaaagtattg
      421 atggtggatg aatttcgttc gcacgcttca attgtggata aaatgagctt tatttgtgcg
      481 gatggcgaga ctggaaaact cattgatatt ttgcctacac gaaaattgtc tcgattaaca
      541 cactatttta gtcgctgtgc aaatcctgaa gcggtggaat atttagtgac agatatgaac
      601 gccgcctact tccaactgac aaaacgtgtc ttaccaaatg cgaaagtcgt cattgattgt
      661 tttcatatca tcaaacattt gaatcaggcg ttcaatgatt ttcgtgtaca agaaatgaaa
      721 agattacacc agttagggca acgacgggaa gccgagaaag tgaaaaagaa ctggcgcttt
      781 cttctaaaaa accgtcaaaa aatcaatcac tatgattaca aaacatggcg cagttttcgt
      841 gcacctaaat acccgctact cactgaagca atgatgattg atcgattgct ggcattttca
      901 acgcctttaa aggacgccta tacttttttc catgagctga ccgaagcctt tagagacaag
      961 gaatcagact tattttttac ct
//
LOCUS       DAYYCY010000319          978 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.327, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000319 DAYYCY010000000
VERSION     DAYYCY010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..978
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.327"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            142..957
                     /gene="thiD"
                     /locus_tag="ACHAZB_002839"
     CDS             142..957
                     /gene="thiD"
                     /locus_tag="ACHAZB_002839"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFM8615439.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT          354 a          152 c          219 g          253 t
ORIGIN      
        1 attgcaaatg ttttttcaaa taaatttgat taccaagaaa aggtagtggt atatttactg
       61 agggatactt tgaaaaacat atgatagaaa cagtaacagt attatgaaaa atctttttat
      121 aaaaatggag gaatcgattt gatggaagat cagtatgtac aagcaatgac gatcgcagga
      181 tcagatagcg atggaagcgc agggatgcaa gcagatatgc atacattctt tacaagaaat
      241 gtttacggtg tttctgtcat gaccgcatgt gttgcaggta attcttacgg aatcggagca
      301 agtgtgacat tgccaacaga ttttatcgac aaagaatttg aactgattgc gaaggacttt
      361 caaatacgtg cagctaaaac aggtatgcta gcagattcaa agttgattga gacagttgta
      421 aaaaattacc aaaaatatga ttttggacca ttagtcgtgg atcccgtaat cgtaacgaaa
      481 catggaaatc ttttactaga agaatctgct ttgcaaagct tgaaagaaaa gcttgtccct
      541 ttggccgaag tactgacacc taatttttat gaagcagaaa agctgactgg acggacatta
      601 gcagctgaag aagattttgt gcaagcagca aaagaactgc aagatatggg tgcaaaaaac
      661 gttatgatca aaggacatca tgctaaaaat gcgcagcaag aaacagtcaa tgactacgta
      721 ttgttcgaat caggaaaatc attttggtta aatgcaccat tttatcctac cgaccggatc
      781 aacggaactg gcgattcgct atctgcatgt attgcagctg agttagcgaa aggaaaaaca
      841 gttgaacaag cgatccgatt ggcaaaagac tatgtaaacc gtgcaattcg tgaagaaata
      901 aatgtagggc acaaatatgg ccccatcaat cattgggcag ctggtgaatt agagtaaaag
      961 aaagaagttg tgaaaaag
//
LOCUS       DAYYCY010000320          959 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.328, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000320 DAYYCY010000000
VERSION     DAYYCY010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.328"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(107..511)
                     /locus_tag="ACHAZB_002840"
                     /pseudo
     CDS             complement(107..511)
                     /locus_tag="ACHAZB_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
     gene            complement(570..>959)
                     /locus_tag="ACHAZB_002841"
     CDS             complement(570..>959)
                     /locus_tag="ACHAZB_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8615440.1"
                     /translation="KDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSL
                     IENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFD
                     TYNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          287 a          170 c          159 g          343 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgagacacg acctaaagat cgtgtctaat
       61 tatgactgta tcagaaaata tgataccaaa cggatctatt cattaattaa ttgacatagt
      121 ttatctgttg ctcttttatg actggataac tgaccaattc atgacgatcc aagtcttctt
      181 tattcatatt tacagcagta atttgactgt cttcatacgt acgataataa taaatccctt
      241 tatcaacgtt acagcaagaa gaataaatcg tatattcgta ttttccttct ccagtatcgc
      301 atactccttt ttgttgctcg actgagccta gtatatgaaa aaattgactg atactttcag
      361 attctgaatc acctgatgca gcattcattt tagtaaatgt ggcttttaca aatcgagaaa
      421 cagaagagag atctccaggt aagcccaatc ctcccatccc acgactataa acattcaagc
      481 tgatctggtt tgaaaaatga ttttctggtg ttccattggg agcgtcaata attttgtgta
      541 aataaattgt cctcctgcaa aataattagt tactcagtaa acattgaaac taatgtatcg
      601 gttacctgtt gaaaaccttt atggcttctg tttagaaatt tttgattgta tgtatcaaaa
      661 atgctgacta gaaagcgttc tagtgattct tcattttgaa actgctcttt tctacggctg
      721 tatcttttaa tttgcttatt gaaagactcg attagattgg ttgagtaaat ggttctacga
      781 atgctaggtg gaaaatcata aaaagttaat aagtcttggt tttctatgag tgactgcgtc
      841 actttaggat agtttttctt ccatttctca atcatgccgg ataagaaggt attcgcttct
      901 tcttttgagt tagcttgata aacagcctta aagtcatcac agatttcttt tcggtcttt
//
LOCUS       DAYYCY010000321          952 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.329, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000321 DAYYCY010000000
VERSION     DAYYCY010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..952
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.329"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>952
                     /gene="bsh"
                     /locus_tag="ACHAZB_002842"
     CDS             <1..>952
                     /gene="bsh"
                     /locus_tag="ACHAZB_002842"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFM8615441.1"
                     /translation="TSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVNDLD
                     THYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFIPW
                     ILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHIYD
                     NPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDLSS
                     VSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSCCN
                     VDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQ"
BASE COUNT          334 a          127 c          170 g          321 t
ORIGIN      
        1 tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg attatgaaat
       61 atcttacaat gaagtagtta ctgttactcc aagaaattat aagttgaatt ttcgaaaggt
      121 aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag ctgactaccc
      181 tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa atttttctgg
      241 gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt ttgaatttat
      301 tccttggatt ttaggacaat gctcaacagt aggagaagct aaaaaattgt taaaaaatat
      361 caatttagca aatataaatt atagtgacga acttccttta tcccctttac attggctatt
      421 agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc atatatatga
      481 taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat ttaatttaaa
      541 caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc aaataagttt
      601 gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat cctcagtatc
      661 tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt cagagtcaga
      721 aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag gtttgtgtga
      781 tgtaggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg ttgacaaagg
      841 aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata tgaataaaga
      901 agacttagat agtcataagt taattagtta tccaattata gaaaaacaac aa
//
LOCUS       DAYYCY010000322          946 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.330, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000322 DAYYCY010000000
VERSION     DAYYCY010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..946
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.330"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>946)
                     /locus_tag="ACHAZB_002843"
     rRNA            complement(<1..>946)
                     /locus_tag="ACHAZB_002843"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          199 a          293 c          214 g          240 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcgg
//
LOCUS       DAYYCY010000323          944 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.331, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000323 DAYYCY010000000
VERSION     DAYYCY010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..944
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.331"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            24..590
                     /locus_tag="ACHAZB_002844"
     CDS             24..590
                     /locus_tag="ACHAZB_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8615442.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            735..822
                     /locus_tag="ACHAZB_002845"
     tRNA            735..822
                     /locus_tag="ACHAZB_002845"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:767..769,aa:Ser,seq:cga)
BASE COUNT          318 a          149 c          197 g          280 t
ORIGIN      
        1 agtaaagaag tgggggagtt ttaatgagaa gaaaagtcta taccaaggat caaattctaa
       61 aagctgctta tgatgttgtt gcaaaggaag ggtttaaagg atttactgcg agaaatattg
      121 ctaaaaagat ggggatttcg acacagccga tttatctgga attcaaaaac atggaagatt
      181 taaaaaatac gttgctggat tctatctttg aacaactaaa agaagatgtt ttccctattg
      241 aacgtactgg tgataaaatc attgatctcg gtttgaacta tatctacttt gcaaaaaagc
      301 atcatagtct ttacatggca ttgtacattg atgaacatgg ggacggaaag aaaatgcatg
      361 atttttcata caattatttc cgtgaaatag ttaaagaaga tgaaagatac aatcaattat
      421 cggatgaata tgtcgatgct ctgcatactg gtacatgggt agcagtgact ggcttagcgt
      481 cattgatgtc atcaaacatc atgcatccaa cagaagaaca aatcattgca ctgatcaaac
      541 ggacaattga ctcgatttta gcattagata atcctgaaat aatcagatag tgttctatca
      601 gtatgaattt gatcggtaag tccgttggct gcaaactgtc aatggacttt tttgctaaaa
      661 aaagcgattt ctgttattta tctattagaa ataggcttga catttcgtga aaaaagttta
      721 aaaatagtac ttacggaagg ttggcagagt ggtaatgcac cggactcgaa atccggcgaa
      781 ccggctaata ccggcgcgca ggttcaaatc ctgtaccttc cttatatcaa cgattagagg
      841 ttgtgaagaa gctgtcctgc atcaagtaat aagaacggcc aaacaaaaat agcttttcct
      901 attttttcgt ttgattgggc ttattaccgc agatgcaagc tttt
//
LOCUS       DAYYCY010000324          931 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.332, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000324 DAYYCY010000000
VERSION     DAYYCY010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.332"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>931
                     /locus_tag="ACHAZB_002846"
     CDS             <1..>931
                     /locus_tag="ACHAZB_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFM8615443.1"
                     /translation="HCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFY
                     RPLNPFKQTLPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFS
                     RFSLATREAVQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETT
                     VRLKGNSHSQRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAA
                     CPNLRQGYQLYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNA
                     LHYSYSNGPLECLNNHIKVLKRNAYGFRNFYNFK"
BASE COUNT          286 a          190 c          180 g          275 t
ORIGIN      
        1 acactgtttc attagtcaaa aagttcgttg gtctgtcatt gctcgcttaa aagagaatac
       61 ttccatgacg gaaatcgctc gtcagaaaaa tatttccact tcttcggtct atcgtgtcat
      121 gaagcgattc taccgcccat tgaatccttt caaacagact ttgccaaagg tcttatgctt
      181 tgatgaattc aaatcggttc gaagtgttgc cagtgcaatg agttttatca tgatggacgg
      241 acaaagccat gcattattgg atattgtcga aaatcgacgc ttgccttatt tagaacgcta
      301 tttttctcgg ttttcattag cgactcgtga agccgttcaa ttgattgtta ttgatatgta
      361 tgcgccttat gtttcgttgg tgaaaaagct tttcccgaat gctcagttga tcatcgatcg
      421 tttccacatc gtccaacata ttggcagaac tttcttgaat catcgagtga aagaaaccac
      481 ggtacgactt aaaggaaatt cccactctca acgagggtta gggaaaaaat tgaagcgcta
      541 ttggaaactc ctacaaaaag aagaagcaaa gttaaactat gaaaaacggg tttggcgtgc
      601 cagttttaaa gattatttga ctgaaagtga gattgccgat cgactgttag ctgcttgtcc
      661 caacttacgc caaggctatc agttatacca agacattctt tatgcggtaa agaaaagaga
      721 tcagtcacta tttgaggatt gtttaactag agacattagc ggtctgccag aaacctatac
      781 aaccacttta cgaacgttta aaaagtttct gccacagatc caaaatgcgc ttcactacag
      841 ctattcaaac ggaccattgg aatgcttaaa caaccacatc aaagtattaa aacgcaatgc
      901 gtacggcttt cgcaactttt acaatttcaa a
//
LOCUS       DAYYCY010000325          924 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.333, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000325 DAYYCY010000000
VERSION     DAYYCY010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..924
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.333"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(243..464)
                     /locus_tag="ACHAZB_002847"
     CDS             complement(243..464)
                     /locus_tag="ACHAZB_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8615444.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(562..807)
                     /locus_tag="ACHAZB_002848"
     CDS             complement(562..807)
                     /locus_tag="ACHAZB_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8615445.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
BASE COUNT          277 a          178 c          114 g          355 t
ORIGIN      
        1 actatatact tcttcccaga acttgtccgt ctccttcaaa ttatctctac ctctcgagtt
       61 cattgggtgg tttgcccatt tattaaacat ggaatcacct ttctcatact catatattta
      121 tgtcctattt caattatatt tatatatgct taaataaatt tgtatcctga aatttattgt
      181 aatatgaagc tgtattttac atatagtaac caaagagtta ataaaataaa aagagcgaac
      241 cattatagat cagctctttt ttcattgctg tttttaatct tatctagaat cacttcaaat
      301 gtttcaaagg tatttacatc taactgacat aaccaaagca agtacttctt ttctttgtaa
      361 gtccaatcaa tattacggta agaggacatt agattatggt accttgctct ttgttcaact
      421 gaagccaatt cccagcgttt ttcaaatatt ttttcttctt ccattattga tccctcgcaa
      481 gacgctacct caaacttaaa taatgggcag cgaaagtcta attccttaga tctaagtata
      541 gcaaacaaat atgaaaaaaa cttagaaggt agtaaaagtt cttccgtctt ttgctttgct
      601 tctattctta atgtgtttcg ttatcggtgc cctacaaaga ttttcattaa ttacacactc
      661 atttattgtt gtgtattttc tgacatttct ctcttgatca attattttaa ttccatgggc
      721 atcctgatag ttcataggag aggagcggct atcagaaata gttgcaccta tctcttgaac
      781 ttcttttaca acttctggaa aatccatcca tcctgcttca gctgtcaaaa gtaataatct
      841 ataatattta tctttccttt tcaatctaag aaatcgtcac cccattcttt ttcttctatt
      901 ataaactatt taacttttag aatt
//
LOCUS       DAYYCY010000326          906 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.334, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000326 DAYYCY010000000
VERSION     DAYYCY010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..906
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.334"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            34..>906
                     /locus_tag="ACHAZB_002849"
     CDS             34..>906
                     /locus_tag="ACHAZB_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM8615446.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDEFFIQSVASKRNN"
BASE COUNT          313 a          157 c          170 g          266 t
ORIGIN      
        1 ttccgaccaa agaattataa ggagtgagta caaatgacct atacccatct tacaacggat
       61 gaacttgtaa taatagagtc ttatttcaaa atgaatcaat ctgttgctaa aactgcccat
      121 tgcttgaatc gttcaagaca aacgatccat aaagtatatc tgttcttcaa gcaaggaaaa
      181 tcagccttag aatattatca acagtataag aaaaacaaat caaactgtgg tagacgtccg
      241 cttgttttac ccgaggaaca atcagaatat attcaaagaa aggttgttca aggatggaca
      301 cccgatgtga ttgttggtcg tgcagcgttt cctattagtt gttctgctcg taccatttat
      361 cgtatgttca aaaaggggct atttgattct tctgacttac cgatgaaagg caagcgtaaa
      421 ccgaatggac atcaagaaag acgtggaaaa caaactttcc gccgctctat tcatgaacgt
      481 gaaaaggatt atagccaatt ctcaaatgag tttggtcacc ttgaaggtga cactatcgta
      541 ggtctgaaac ataaaagtgc tgtaattacc ttagttgaac gattatcaaa agttatcatc
      601 acattgaaac cgtgtggtag acaagcgatt gatattgaaa aaaaattaaa tcaatggttt
      661 gaatctgtac cgaaaaacct attcaaatcc atcacttttg attgtggaaa ggaattttca
      721 aattggaaac agatcagtaa tgtcaatgat attgccattt atttcgctga tccaggaacg
      781 ccgtctcaaa gaggcctaaa cgagaattct aacggattgt tacgtagaga tggtttattg
      841 aaatctatgg atttcaattc agtagatgaa ttttttattc aatctgtcgc atctaaacga
      901 aataat
//
LOCUS       DAYYCY010000327          902 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.335, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000327 DAYYCY010000000
VERSION     DAYYCY010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..902
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.335"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            136..309
                     /locus_tag="ACHAZB_002850"
     CDS             136..309
                     /locus_tag="ACHAZB_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8615447.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
BASE COUNT          302 a          145 c          109 g          346 t
ORIGIN      
        1 ttttcggaga attaaaaaat acaataaata actttagtta ctttaatgcg ttcttacttt
       61 atttttatat tatcattttt cttattcact tctttggttt atactattct tataaataaa
      121 aacgaggtat gtcctatgat gaaaagattg tactattcat tgatcataac gattggctat
      181 ttgattgtat caaatctagg aaatatggta tttggtatca gtaaagaatt cagttggact
      241 actacattat gggaatctct attctttttc atcttcgtat ttttgttaca aaattatcga
      301 aaaaaataaa catgtgccat ctatctgttg gcacatgttt taggatttat ggtacttttt
      361 attcggcaat ccattttatt tttctacttc ttcttaatac ctctaacact tttgatttat
      421 aaactaatcc aatgcatcta cttgcgctta ttaagctatt tcagctagat agtaccaccc
      481 catgcgtcaa tgcttgattt tccttttgta taacattgta taagcaataa aggacaaata
      541 agtggtgatt acaccaaacc attgaataag aaaaattctg atacgtaaat aaacatgaac
      601 ttgaaaaaat taatgaatcg gttagctact acgtagttgt cttttttctc atacagttct
      661 gttaacttac acttctcttc gctagataat aaaaaatcct ccaactgtat tgtccaatat
      721 aatcccaggc atctaaacta gcattataga agagacacac atagaatcta aaataactgg
      781 ttttaaatgt ttcttacaat caaaaaaagc tgttaaatgc actccacacc ttcatactct
      841 atcatttttt ataaacgata tatttttatt gtttttcgat aaaaaatagt tgttttacaa
      901 ta
//
LOCUS       DAYYCY010000328          896 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.336, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000328 DAYYCY010000000
VERSION     DAYYCY010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..896
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.336"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            41..874
                     /locus_tag="ACHAZB_002851"
     CDS             41..874
                     /locus_tag="ACHAZB_002851"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFM8615448.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
BASE COUNT          237 a          164 c          179 g          316 t
ORIGIN      
        1 tatactataa gatgtatctt aattgggaag gaaggaattt atgttttttg caaatattat
       61 caaagcagtg attctcggta ttattgaagg aataacggaa tggctgccca tcagtagtac
      121 tggtcatttg atcctagcag atgaattcat caaactggat gccagttcac aatttatgtc
      181 gatgttcaat gtcgttatcc aattaggggc gatcttggct gtcgtcgttc tttattttca
      241 caagttaaac ccattttctc caagtaaatc aacggtagaa aaaaaagata cgtggacact
      301 ttggtttaaa gtgatcgttg cttgcatacc agccatcatc attggtttgc ctttggatga
      361 ttggttagaa gcaaatttcc acaagttctt accagttgcc attgttttga tcgtttacgg
      421 tattgccttt attattgtcg aaaaacgcaa caaaaacaaa acacctcgtt ggacttctct
      481 gaatgacttt acgtaccaag cagctcttct tgttgggtta ttccaagtct tggcacttgt
      541 tcccggaaca tcccgttctg gcgcaacgat cctaggatct atcttgattg gtgcttcgcg
      601 ttttgtcgct acagagtttt catttttctt agggattcct gtcatgtttg gcgcaagcgg
      661 cttgaagatc gtcaaatatt tagctgaagg gaattcattc aaaatggaag aaactgttgt
      721 tcttcttgta ggaacattcg tatcttttgt ggtttccgtc ttagctatca aattcttact
      781 aagctatttg aagaaaaacg actttaccgc ttttggctgg tatcgtatta tccttggtat
      841 cattttgatt ggttattggt ttattgcaat gtagaaaaaa ccagaacact tctctt
//
LOCUS       DAYYCY010000329          895 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.337, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000329 DAYYCY010000000
VERSION     DAYYCY010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..895
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.337"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>895
                     /locus_tag="ACHAZB_002852"
     CDS             <1..>895
                     /locus_tag="ACHAZB_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HFM8615449.1"
                     /translation="SFIAKNCQVSLTTVIRTLKEFKSYLPKQSKRILPRVLMVDEFRS
                     HASIEDKMSFICADGETGQLIDVLPTRKLPRLTSYFLGCANPEEVKFLVTDMNAAYFQ
                     LTKRILPNAKVVIDRFHIIKHMNQAFNELRIREMNELRKVGQKSQAEKLKKNWRFLLK
                     NRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLGFSTSLKEAYPYFHELVEAFRDKD
                     PDLFFSLLAELPETLDDGFREKLQNLLTYEEGITNAMIYPYTNGKIEAKNTHIKTMKR
                     VSYGFKSFENMRIRIFLINQL"
BASE COUNT          300 a          175 c          168 g          252 t
ORIGIN      
        1 tatcttttat tgcgaaaaat tgtcaggtat ctttgaccac cgttattcgt acgttgaaag
       61 agtttaaaag ctacttacca aagcaatcta agaggattct ccccagagta ttgatggttg
      121 atgaatttcg ttcgcacgct tccatagaag ataaaatgag ctttatttgc gcagatggcg
      181 aaacaggaca attaattgat gtcttgccta cgcgtaaatt gcctcgatta acaagctatt
      241 tcttaggctg cgctaatcct gaagaagtaa aatttttagt cacagacatg aacgccgcct
      301 acttccagct taccaaacgt attctgccaa atgcgaaagt agtgattgat cggtttcata
      361 ttatcaaaca catgaatcaa gcctttaatg agttgcgtat ccgtgaaatg aatgaacttc
      421 gtaaagtagg acagaaaagt caggcagaaa aactgaaaaa gaactggcgc tttttgctaa
      481 aaaatcgtgc aaacatcaac cattatgaat acaaaacatg gaaaagtttt cgagcaccaa
      541 aatacccatt tcttacggaa gcgatgatga ttgatcgatt gcttggcttt tctacgtccc
      601 tgaaggaggc gtatccctat tttcatgaat tagttgaagc ctttcgagac aaagaccctg
      661 acttattttt ctccttattg gcagaacttc ccgaaacgtt agatgacggc tttcgggaaa
      721 agctccaaaa ccttctgacc tatgaagaag gcatcaccaa cgcgatgatc tatccttaca
      781 ccaatggaaa aatagaagcg aagaataccc atataaagac aatgaaacga gtatcctacg
      841 gatttaaatc atttgagaac atgagaatta gaatcttttt gatcaatcaa ttaat
//
LOCUS       DAYYCY010000330          862 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.339, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000330 DAYYCY010000000
VERSION     DAYYCY010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..862
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.339"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            87..356
                     /locus_tag="ACHAZB_002853"
     CDS             87..356
                     /locus_tag="ACHAZB_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFM8615450.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            349..606
                     /locus_tag="ACHAZB_002854"
     CDS             349..606
                     /locus_tag="ACHAZB_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFM8615451.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
BASE COUNT          328 a           95 c          164 g          275 t
ORIGIN      
        1 tataagaagt tagaatatta atcatttgct taattgtaca atataatgta caattgtttt
       61 atagaaataa ataaggggtg aaaggaatgg aagcagtagc ttattcaaat ttccgccaaa
      121 atttacgtag ttatatgaaa caagttaatg aggatgctga aacacttatt gtaacaagta
      181 aagatgtaga agatacagtt gttgtattat caaaaagaga ttatgattct atgcaagaaa
      241 cgttgagaac actttctaat aattacgtca tggaaaaaat tcgtcgagga gatgaacaat
      301 tctccaaagg tgcatttaaa acacatgact taatcgaggt tgaatctgat gattaaggct
      361 tggtctgatg atgcttggga tgattatctt tattggcatg agcaaggaaa caaaagcaat
      421 ataaaaaaga ttaacaagtt aataaaagat atcgatcgtt ccccctttgc tggattagga
      481 aaacctgagc cattaaagca tgatttatct ggaaaatggt ccagaagaat tacagatgaa
      541 catagactga tatatagagt tgaaaatgaa acgatattta tttattctgc aaaagatcac
      601 tattaaccaa tcggaagtaa ggaaagggtc agaaacttaa aagtttttga tccttatttt
      661 atttacccta gtcatttaaa aagctaatat agcttagtgt tgattgttat taatgaatgt
      721 gtttgttacg cgtattacgg atataaggtt agtaaaatca tttctaaagt tgaggaaaag
      781 taaatataaa tggcttaaat ttcaacaatt tgaagttgaa tagatatgtt ataatactat
      841 tgtagtgtgg gatgttagtt ac
//
LOCUS       DAYYCY010000331          849 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.340, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000331 DAYYCY010000000
VERSION     DAYYCY010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..849
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.340"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>849
                     /locus_tag="ACHAZB_002855"
     CDS             <1..>849
                     /locus_tag="ACHAZB_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFM8615452.1"
                     /translation="QINNMLNLNLKTYELNKIDLSLSHDFKINEVQYTQVSEMYHTML
                     EKNKEIDFDMILLVAYKILTVNSNVARNIASNIRAIYVDEFQDTRELQYNVIAKLLQN
                     NPQIQSMFVGDIDQAIYGSLDGIAKSVEELEMLTSHTFQSKTLHGCYRSNQRLVDFYS
                     NFQSCPYEIESRGNNKNDRGFISYDCKVTKEDLPNIIKNIIKEKIESGIHEKDICVLA
                     PQHYPLFSLGEYLKKELPYCNFDAINISPIKVNNHSLFFKLAILYFTESGEKVRRRKL
                     IANDILI"
BASE COUNT          322 a          112 c          140 g          275 t
ORIGIN      
        1 caaattaata atatgctgaa tttaaatctc aaaacgtatg aactgaacaa aattgatttg
       61 tcactttctc atgattttaa aattaatgaa gttcagtata ctcaagtttc tgaaatgtat
      121 cacactatgt tggaaaaaaa taaagaaatt gattttgata tgattttact agtagcgtat
      181 aaaattttaa ctgtaaacag taacgttgca cgtaatattg ctagtaatat aagggcgatt
      241 tatgtggatg aatttcaaga tactagagaa ttacaataca atgtaatagc taaattactt
      301 caaaacaacc ctcaaattca gtcaatgttt gttggagata ttgaccaagc aatttatgga
      361 tcattagatg gtatagctaa atcagttgaa gaactagaaa tgctaactag tcacactttt
      421 caatcaaaga cgctacatgg ctgttacagg tctaatcaga gattagtaga cttctactct
      481 aattttcaga gttgtcctta cgagattgaa agcagaggga ataataaaaa cgaccgtggt
      541 tttataagtt atgattgcaa agttactaag gaggatttac ccaacataat aaaaaatata
      601 ataaaggaaa agattgaaag cggcattcat gaaaaagata tttgtgtact tgctcctcaa
      661 cactatcctc tgttttcact gggtgagtat ttaaagaaag aattaccgta ttgcaatttt
      721 gatgctataa atatatcacc aattaaggta aataatcata gtttattttt caagttagca
      781 attttgtact ttactgaatc aggtgaaaag gttcgaagaa gaaagctaat tgcaaacgat
      841 attttaatt
//
LOCUS       DAYYCY010000332          846 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.341, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000332 DAYYCY010000000
VERSION     DAYYCY010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..846
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.341"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            117..656
                     /locus_tag="ACHAZB_002856"
     CDS             117..656
                     /locus_tag="ACHAZB_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8615453.1"
                     /translation="MWSLVGIVLIIFTVTAATEANYSHKIQSYKGRLVMTKKDVTRYT
                     SSRTGGLYDYAPLDALKYKDHLKKHEVKVGDRWENASHKAYNSKIVYTVEDADEQNIT
                     LPVFNYWGTVVKVNGKKSTIENDNGLVSIKGTGKRTIVEVSSTYPTTMILSLLISSIT
                     FIFLFTRYLIRCCGEMKID"
BASE COUNT          320 a          110 c          153 g          263 t
ORIGIN      
        1 gcgtttgatt ttcaaacttc gcaacagaac cgagaacctt gggttttcaa gtgctttttg
       61 gttcattaat cttaaccata gtgtttttaa agtggaaaac aagcaacaaa aaaagtatgt
      121 ggagccttgt aggaatagtt cttattattt ttactgtgac tgcagcaaca gaagcaaatt
      181 attcgcataa aattcagtcg tataaaggtc gtttagttat gaccaaaaaa gatgttacac
      241 gttatacatc ctcaagaact ggaggcctat atgactatgc accattagat gcattgaaat
      301 ataaagatca tttaaagaaa catgaagtaa aagttggtga tcggtgggag aatgcatctc
      361 ataaagcata taattccaaa atagtctata cagtagaaga tgccgatgag caaaatatca
      421 cattacctgt attcaattat tggggaacag ttgtgaaagt aaatgggaaa aagagcacta
      481 tagaaaatga caatggttta gtgtctatta aaggtacggg gaaaagaact atagttgagg
      541 tttcatctac gtatccaact acgatgattc tatctttatt aattagttca ataactttta
      601 tattcttatt tacacgttat ttaataagat gctgcggtga aatgaaaata gattagatag
      661 atattataaa tatgttgatt ctattttcat agcaatggag aaaatacatt agcaatatat
      721 aaaagaaggt atcaagagtc acagtgaatt tattccgaaa agtgaaaaga gcaaatataa
      781 aatttaatga aaatatagaa ctaaatagga aaacgtagac gtaattttct acgttttcct
      841 tttcat
//
LOCUS       DAYYCY010000333          842 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.342, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000333 DAYYCY010000000
VERSION     DAYYCY010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..842
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.342"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            34..705
                     /locus_tag="ACHAZB_002857"
     CDS             34..705
                     /locus_tag="ACHAZB_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8615454.1"
                     /translation="MTIKTSLQNQAYQSIRQQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRHQGLVDTIPQSGNYISLIDLDLAKNARFVREHLERQIMLECCAKMNNQ
                     KKQVLETIIAEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLEDNNTHLERF
                     RWLRVKTEGLKWEKIMEQHHQLFKALTMQDPEEANFLTSLHLHLMLDEQNVVLTTHPK
                     YFKNI"
BASE COUNT          318 a          126 c          132 g          266 t
ORIGIN      
        1 aattcaaaaa tgaaattttg agagggatta aatatgacaa ttaaaactag tctccaaaat
       61 caagcttatc aatctattcg tcagcaaatt atctacgctg acttagaacc tggaaaaaaa
      121 gtatcagaaa aaaaattgga agaaacgctt tctatcggtc gtactccaat acgtgaagca
      181 ttgattcaac tccgtcatca aggacttgtt gatacaattc ctcaaagtgg aaattatatc
      241 tcattaatcg acttagattt agcaaaaaat gctcgttttg tcagagaaca tttagaacga
      301 caaattatgc tagagtgttg tgccaagatg aataatcaaa agaaacaagt tttagaaact
      361 ataattgcag aacaagaaaa agctgttagt caaaagaatg ctcgtagttt ttttcataat
      421 gataatttat ttcataaagc ttgttttgaa attgcagaac gggaagaagt atggcaatgg
      481 ttggaagata ataatacgca cttggaacga tttcgttggt tacgagtaaa aacggaagga
      541 ttaaaatggg aaaaaattat ggaacaacat catcaactat ttaaagcctt gaccatgcaa
      601 gaccctgaag aagctaactt cttaacatcc ttgcatcttc atttaatgct tgatgaacaa
      661 aatgttgtat tgactactca cccaaaatat tttaaaaata tataactgct aaatttcttt
      721 tttttaattt atttacattt ctgattacta cagaaaacta actacttttt ttatgtaaag
      781 ttctgtcaaa tatagtcact ttatattact ttctcttgag taaaaaaaca tgtgaggtcg
      841 ta
//
LOCUS       DAYYCY010000334          816 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.343, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000334 DAYYCY010000000
VERSION     DAYYCY010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..816
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.343"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            8..81
                     /locus_tag="ACHAZB_002858"
     tRNA            8..81
                     /locus_tag="ACHAZB_002858"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42..44,aa:Asn,seq:gtt)
     gene            121..210
                     /locus_tag="ACHAZB_002859"
     tRNA            121..210
                     /locus_tag="ACHAZB_002859"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:155..157,aa:Ser,seq:gga)
     gene            225..296
                     /locus_tag="ACHAZB_002860"
     tRNA            225..296
                     /locus_tag="ACHAZB_002860"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:258..260,aa:Glu,seq:ttc)
     gene            312..384
                     /locus_tag="ACHAZB_002861"
     tRNA            312..384
                     /locus_tag="ACHAZB_002861"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:345..347,aa:Val,seq:tac)
     gene            394..466
                     /locus_tag="ACHAZB_002862"
     tRNA            394..466
                     /locus_tag="ACHAZB_002862"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:427..429,aa:Asp,seq:gtc)
     gene            502..577
                     /locus_tag="ACHAZB_002863"
     tRNA            502..577
                     /locus_tag="ACHAZB_002863"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:535..537,aa:Phe,seq:gaa)
     gene            579..659
                     /locus_tag="ACHAZB_002864"
     tRNA            579..659
                     /locus_tag="ACHAZB_002864"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:613..615,aa:Tyr,seq:gta)
     gene            666..739
                     /locus_tag="ACHAZB_002865"
     tRNA            666..739
                     /locus_tag="ACHAZB_002865"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:698..700,aa:Trp,seq:cca)
     gene            742..814
                     /locus_tag="ACHAZB_002866"
     tRNA            742..814
                     /locus_tag="ACHAZB_002866"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:775..777,aa:His,seq:gtg)
BASE COUNT          178 a          188 c          224 g          226 t
ORIGIN      
        1 atgttattcc ggcatagctc agttggtagt agcgcatgac tgttaatcat gatgtcgtag
       61 gttcgagtcc tactgccgga gttctcattt tgagaaagag tattgagctt ctttcactct
      121 ggagagttgt ccgagaggcc gaaggagcat gattggaaat catgtagatg gtgccaaact
      181 gtctcaaggg ttcgaatccc ttactctccg tagcaaattt acctggcccg ttggtcaagc
      241 ggttaagaca ccgccctttc acggcggtaa cacgggttcg aatcccgtac gggtcatttt
      301 aattgaatgt tggaggttta gctcagctgg gagagcatct gccttacaag cagagggtca
      361 gcggttcgat cccgttaacc tccataatga actggttccg tggtgtaggg gttaacatgc
      421 ctgcctgtca cgcaggagat cgcgggttcg attcccgtcg gaaccgtcgt tcttgttatt
      481 caattaataa ttttaaaata tggcgcggta gctcagttgg tagagcaacg gattgaagct
      541 ccgtgtgtcg gcagttcgat tctgtcccgc gccaccatgg aggagtagcg aagtggctaa
      601 acgcgacgga ctgtaaatcc gttccttagg gttcagtggt tcgaatccac tctcctccat
      661 tttatagggg catagtttaa aggtagaaca gcggtctcca aaaccgttag tgtgggttca
      721 attcctgctg cccctgccat ggcgaatgtg gcgaagtggt taacgcaccg gattgtggct
      781 ccggcattcg tgggttcgat tcccatcatt cgccct
//
LOCUS       DAYYCY010000335          770 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.344, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000335 DAYYCY010000000
VERSION     DAYYCY010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..770
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.344"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            200..>770
                     /locus_tag="ACHAZB_002867"
     CDS             200..>770
                     /locus_tag="ACHAZB_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HFM8615455.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNG"
BASE COUNT          265 a          115 c          141 g          249 t
ORIGIN      
        1 ttcggaataa ggggctgtga caagactttt gtcacagccc cttattgttg tacttttaac
       61 ctacaatatt ttttgattcc ccctgagtac tattttttat aaatgctagt aaatcaatct
      121 tctttggcct ttttttgata aaaaaaatac tagatgtaag ataaaagtgt aaaaacgatt
      181 tgttttagga ggggaaacga tgtgtaccgc aatcacttat gcaacgaaag accattattt
      241 tggaaggaat tttgattatg agatgtctta taatgaagtg gtcactatta ctccaagaaa
      301 ttatcgtttc gattttagaa aggtaaaaaa tttagataaa cattatgcaa tgataggaat
      361 agcagctggg gtatccaact atccactcta ttatgaggct acaaatgaaa aagggttgag
      421 tatggcagga ctgaattttc ctggcaatgc tgattataaa gaattacaag aaggaaaaga
      481 taacgttgca ccatttgagt ttattccatg gatactaggg caatgctcaa ccatagacga
      541 agctaaagaa ttattggcta ctatcaatct agtcaatatc gattttagtg aaaaactacc
      601 cttgtcacct ttacactggt tattagctga taaagaaaaa tcgatagtaa ttgaaagtat
      661 gaaagatgga cttcatttat atgataatcc tgtcggtgtg ttgacaaata atccgccatt
      721 tgattatcaa ttatttaatc tgaacaatta tcgctcgcta tcaaatggaa
//
LOCUS       DAYYCY010000336          757 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.345, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000336 DAYYCY010000000
VERSION     DAYYCY010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..757
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.345"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..704
                     /locus_tag="ACHAZB_002868"
     CDS             <1..704
                     /locus_tag="ACHAZB_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFM8615456.1"
                     /translation="YSVIFMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAP
                     TESAYIWKEVLQDLRKRGLEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIA
                     HKVRVRGRKEICEDFKLVYQANSKEEALDHIDFMTRKWKKQYPRVVNLLLNPALLTFY
                     NFPHAIRRTIYSTNLIEGFNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRI
                     HKGFKEIQDTLESMI"
BASE COUNT          255 a          123 c          145 g          234 t
ORIGIN      
        1 attattccgt tatatttatg gatgcgacgc atatccctgt gaagcgacag actgtttcga
       61 aggaagcagt gtatattacg attggtattc gtttggatgg aaccaaagaa gttcttgggt
      121 ttactattgc cccaactgag tctgcttata tttggaaaga agttcttcaa gatcttagaa
      181 aacgtgggtt agaagaagtt ttattagtag tgacagacgg attaagctgt attgaagaaa
      241 gtatccatag tgtgtatccg aatgcccaat ttcaacaatg ttgtgtgcat gtatctagaa
      301 atatcgctca taaagttcgt gttcgaggtc gaaaagaaat ttgtgaggat ttcaaattgg
      361 tttaccaagc gaattcaaaa gaagaggcat tggatcacat cgactttatg actaggaaat
      421 ggaaaaagca gtatccaaga gtcgtcaatt tactcttgaa tcctgcccta ttaacctttt
      481 ataatttccc tcacgccatc agacgaacaa tttattcgac gaacctgatt gaaggcttta
      541 ataagcagct aaaacgatat actcgaagaa aagaacaatt ccctaatgaa gaatctctag
      601 agagattctt catttctcaa tttaatcaat ataaccaaaa atttttaggt agaattcaca
      661 agggatttaa agaaattcag gatacattag agtcgatgat ttaactgtaa ttaacagaat
      721 ggattttcca tttacatata attcttgacg ctaccat
//
LOCUS       DAYYCY010000337          736 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.347, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000337 DAYYCY010000000
VERSION     DAYYCY010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..736
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.347"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(109..>736)
                     /locus_tag="ACHAZB_002869"
     CDS             complement(109..>736)
                     /locus_tag="ACHAZB_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HFM8615457.1"
                     /translation="TSTYTIAHFKGQRTACNEAKTANVLDRTFTQEQPLEAIVTDLTY
                     VRVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSLTDVKLFHTDRGK
                     EFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQFETLAQLRLE
                     LFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT          207 a          167 c          139 g          223 t
ORIGIN      
        1 gacaaaaatt ataaggtgtt gtgaatgctg cggtgttatg gctacgattg cccttgacaa
       61 tgagcatcct cggatctgat tttctccggc agaagcacaa tcattcaatt acgctgcctc
      121 tccagaggta tcagatccgc gctcattatc aaggattcgc ttcgccaatc tctgttgtcg
      181 aatttggatc ggtgtttcat acgccaacgt gccatgtaag cgtagatagt tccaccaatg
      241 cacataatca aacaattcta gacgtagctg tgccagtgtc tcaaattggt attgatgcac
      301 gaattctact ttgacagatt tataggttga ttccacaacg gcattatcat acggacaacc
      361 cttcctactc aatgaacgag taattccaaa accattcaga atctcatgaa tggtttggtt
      421 atcaaattcc tttccccggt ctgtatgaaa aagcttgacg tctgttaaag aatacggtat
      481 ccgtccaaag gcttctttta ccaatgaggc atctttcttc tcaccacagg aataaccaat
      541 aatttctcga ttaaacaaat caagtattaa gcagatataa tgccactttt tccccacgcg
      601 aacataagta agatccgtaa cgatggcttc caatggctgt tcttgtgtaa aggtccgatc
      661 taatacattc gctgttttcg cttcattaca agctgttcgt tgccctttaa aatgagcgat
      721 cgtataggta gatgtc
//
LOCUS       DAYYCY010000338          733 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.348, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000338 DAYYCY010000000
VERSION     DAYYCY010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.348"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>733
                     /locus_tag="ACHAZB_002870"
     rRNA            <1..>733
                     /locus_tag="ACHAZB_002870"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          226 a          146 c          216 g          145 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga att
//
LOCUS       DAYYCY010000339          714 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.349, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000339 DAYYCY010000000
VERSION     DAYYCY010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..714
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.349"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..448)
                     /locus_tag="ACHAZB_002871"
     CDS             complement(<1..448)
                     /locus_tag="ACHAZB_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8615458.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRKL"
BASE COUNT          216 a          124 c           95 g          279 t
ORIGIN      
        1 tcaactttct cttttcaaaa aaaatttgat atttttttag atttcttttt agtctttgta
       61 atactttata agccacagaa taactcacgt gatgctgttt agcaaaattt tctactgaga
      121 tatatttttc ctgaaagaaa gccaatacca ttttcataga taaagaatcc gttatataac
      181 gagataacag agctttgcta gaacattttc cattaatttc cagacatata tccaattcac
      241 tgacggttac tcgcaaattt ttctctagat cgaacttttg taaatcatgt ttcagcttat
      301 cgatcgtttt tttcaagaga aatgtcgata gcgacaactc ttcttgaaca ttcgatattg
      361 actctgataa atttgagctt gcttctaaat atttaagtaa tgaataactt ctgtgatctt
      421 ctttgcttaa aaaaaactca taattcatta gtcatcagct ctttctacgt atattacttt
      481 ccatgcattc accaactagc tggggataaa actgatttcc tggatccgag tcactgttgt
      541 cagcgattct tgtttgtata atatacatcg tcctgtcttt tttttaacat tttggagagc
      601 gttttatttg gcctttctcg cgatcaaacc tgttattaga attatatcat ttactagtat
      661 gcatttttat taataaaatg gtatatttta tattgttata aaaagagccc aacc
//
LOCUS       DAYYCY010000340          714 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.350, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000340 DAYYCY010000000
VERSION     DAYYCY010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..714
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.350"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(452..676)
                     /locus_tag="ACHAZB_002872"
     CDS             complement(452..676)
                     /locus_tag="ACHAZB_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8615459.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
BASE COUNT          261 a          100 c          131 g          222 t
ORIGIN      
        1 ttttgtaaaa aagtgggttg agagaaatct aattgatttt caaagaagga gtatgaaaat
       61 gagaaaaata tttattcgtt tattgtgggt actatcttag aggaataaat aatagtggag
      121 aaaactatgg tttggcgcag attctatcag gtttgatgga acgaagacaa cctcccgaca
      181 tcaagtcaaa agctacttga gattttgatc tgaaaataaa aaaagaaaac aaggtaggaa
      241 catcaatgtt tctaccttgt tttcgtaatc aatgggaaaa aactaagagt ggtgtacgta
      301 atcaataaaa aggatggtga caacagtacc tctttactct ctgaaagcat cataactcgt
      361 ttttcctcat tatgccaata aattgttgtt ataccacata taaggatttg taatagctaa
      421 aaacagacag gaaagttgaa atctgtgaat cttattttgt atggaaggat ttatagaacg
      481 caaaaatcaa gataaagatg aataaaaatc ctgtttcggc tatatattgc gtatctacgc
      541 caataaaacg agtttgttct gtcatagcca tataaagcaa aggcaaaaat acaactacag
      601 cgagataaga tccgatgatt tctcgaaaag ttttccagga agtatgtttc ctgtttttat
      661 atttatccga tttcattgaa tagtcatagc ctccttattc tttcatatgt tgaa
//
LOCUS       DAYYCY010000341          710 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.351, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000341 DAYYCY010000000
VERSION     DAYYCY010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..710
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.351"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            286..684
                     /gene="spxA"
                     /locus_tag="ACHAZB_002873"
     CDS             286..684
                     /gene="spxA"
                     /locus_tag="ACHAZB_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SpxA"
                     /protein_id="HFM8615460.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT          252 a          101 c          131 g          226 t
ORIGIN      
        1 tttcatttag cttttgagat aaaataatag tacagtttta tacgctgttt catatgttta
       61 gaaaatttga attagatttg aaaactctaa tttttaaatg ataaaaaaag cgagaatgct
      121 ggttttactg tatattaacc ttagataact ttcatttttt taaaaaaatt atgaaaaaaa
      181 ggtagctatt taaaagttga ttatgtataa tagaaacaga tgggagatta ttatgatctt
      241 ctgttacaaa aaagtgattc ttatcacgaa aaggagtggt gcacgttgtt aacattatat
      301 acttccccaa gttgtacttc ctgcagaaag gctcgtgcgt ggttgcaaga acatcagatt
      361 ccatttgttg aacgtaatat tttttcagaa ccattgaaca gttctgaatt aaaagcgatt
      421 ttacaaatga cagaagacgg aacagaagaa atcatctcga cacgttccaa agtattccaa
      481 aaactgaaca tggatttaga tgatctgccg ttacaagaat tattggaact tgtgcaaaac
      541 aatcctggat tgcttcgccg tccaatcatg attgatgata aacgtctgca agttggtttt
      601 aatgaagatg aaattcgtcg gttcctaccg cgcgatgtac gacaattaga attacgtcaa
      661 gcacaattga tggctggatt atagaaggat aatacgaaac ttggaaaaga
//
LOCUS       DAYYCY010000342          704 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.352, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000342 DAYYCY010000000
VERSION     DAYYCY010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..704
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.352"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          298 a          106 c          122 g          178 t
ORIGIN      
        1 aattccttag gcagcttttg ctgtgtagaa aggaataaat aataacgaaa gttcaaaagc
       61 taatgccgac tattctagac attaaagaag acgaaaacga acgataaaga aagaaaagtt
      121 atgaataatc gtttattgaa aaaaacttga aaaaatacaa aaaattcgtt gaccaagcta
      181 aatttagttg gtatattatt acttgtccaa aagacagaag aaaatattta ttcaaaaaca
      241 taacaaaaaa gttattgaca tgttactaac gacatgataa gataactgag tcgttaaaaa
      301 agaaccactt gcgaagcttg attctatcga aaaaaacttc aaaaaagttc ttgacaaata
      361 acaaacgata agttatgata aatgagttgc cgaaagattt cgaaaaacga aatcgaaaaa
      421 aacttttaaa aaagagttga caatcgcttg tctctctgat atgatataaa agttgctacg
      481 gcagccaaaa atgaatcggg aaacactctc gatgaagtag acctttgaaa actgaacaaa
      541 gtaagacaaa ccaaatgtgt agggcgtctt gattcaattc aagacaacaa acatttttaa
      601 caagcaagca atatgctagc aaacaaattg agcttaacaa tcgcaagatt gttcgaactt
      661 tttatgagag tttgatcctg gctcaggacg aacgctggcg gcgt
//
LOCUS       DAYYCY010000343          652 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.353, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000343 DAYYCY010000000
VERSION     DAYYCY010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..652
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.353"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..635
                     /locus_tag="ACHAZB_002874"
     CDS             <1..635
                     /locus_tag="ACHAZB_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFM8615461.1"
                     /translation="EITYENILKDINFDVKVGDVIGLVGKNGVGKTTLLRILMKIIRP
                     TKGKIIENKKNLSSPFLVMQEMDYQFFTESVRSELALGNEIVSKDQQEKILKKMELYK
                     MKDQLPFDLSGGEKQRLLVSIASLSKTNLFLFDEPTSGLDYINMERIADLIKDLKEKG
                     AVVIATHDPEFLYKTCNRIIYLKNGGIKKDIKLRVEDSTLIEQIFNDITK"
BASE COUNT          267 a           69 c          122 g          194 t
ORIGIN      
        1 aagaaattac ttatgaaaat attctaaaag acataaattt tgatgtcaaa gttggagatg
       61 ttattggttt ggtagggaaa aacggtgtgg ggaaaacaac cttactaaga atactcatga
      121 agattattcg ccctaccaaa ggaaagataa tcgaaaataa aaaaaatttg tcatcgccgt
      181 ttttagtaat gcaagaaatg gattaccaat ttttcacaga atcagtgagg agcgagttag
      241 ccctggggaa tgaaatagtt agtaaagatc agcaagaaaa aattttaaaa aaaatggaac
      301 tttataaaat gaaagatcaa ttaccgtttg atttatccgg tggagaaaaa caaagattat
      361 tggtttctat agcttcattg tcaaaaacta atttatttct atttgatgaa cctacaagtg
      421 ggttggatta tattaatatg gaaaggattg ctgaccttat taaagactta aaagaaaagg
      481 gtgcagtagt tatagctact catgatccgg aatttcttta taaaacatgc aatagaatta
      541 tttatttaaa aaatggtgga ataaaaaaag atataaaact tagagtagaa gattcaactt
      601 tgatagaaca gatttttaat gacataacga aatagaattg aggagaatat at
//
LOCUS       DAYYCY010000344          646 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.354, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000344 DAYYCY010000000
VERSION     DAYYCY010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..646
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.354"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..643
                     /locus_tag="ACHAZB_002875"
     CDS             <1..643
                     /locus_tag="ACHAZB_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8615462.1"
                     /translation="FEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFS
                     MWDTFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVE
                     FSADKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQ
                     NGYLDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
BASE COUNT          209 a           94 c          115 g          228 t
ORIGIN      
        1 atttgaaatc ccttattttc tggaaaagat attaaagata cagattccaa atatgctaga
       61 aatcattttg ttatctttta tttttagttc aacagtttta ggagagttaa gtgattttta
      121 tggctatttt tcaatgtggg atacttttct acatgcattc agcggatttt tagctggggg
      181 tgtcggcttt tcccttgttt atttgctcaa taaaaataca aaaaccatga acttgactcc
      241 tttattacta gcagttgttt ccttctgttt ttcgatgaca gttggtgtta tgtgggagtt
      301 tgtggaattt tcagcagata aattactgca attcgatatg caaaaagata cgatcatcca
      361 gcaaatcaat agtgtggagt taagtaaaga cggaaacact gtctatcatg ttccagatat
      421 cactcggaca atcattgaaa gtcaaaatgc agatggtaaa acagataaaa caatcatcca
      481 aaatggttac ttagacgtgg gaatcatgga tactatgaag gatttatttg ttaattttat
      541 tggagcagtt gttttcagtg tatttggtta cctctatagt cgaaacgatt accaaaaatt
      601 tacgttcatc aagaatttta ttcctaaaaa aatcaaacga taaaac
//
LOCUS       DAYYCY010000345          635 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.355, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000345 DAYYCY010000000
VERSION     DAYYCY010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.355"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..220
                     /locus_tag="ACHAZB_002876"
     CDS             <1..220
                     /locus_tag="ACHAZB_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFM8615463.1"
                     /translation="KFQLFTLDECINVDLLNNKISFEENIVKEMPESANDMYEKELKY
                     FFENVVTGKENWNDLYHANEVLKIAEEK"
     gene            221..>635
                     /locus_tag="ACHAZB_002877"
     CDS             221..>635
                     /locus_tag="ACHAZB_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFM8615464.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDV"
BASE COUNT          248 a           70 c          116 g          201 t
ORIGIN      
        1 aaaatttcaa ttgtttactt tagatgaatg tatcaatgtt gacttattaa ataataaaat
       61 aagttttgaa gaaaacattg ttaaagaaat gcctgaatct gctaatgata tgtatgagaa
      121 ggagttgaaa tatttctttg aaaacgtagt aacaggcaaa gaaaactgga atgatttata
      181 tcatgcaaac gaagtattga aaatagctga ggagaaataa atggaaattt tatttactat
      241 ttgtggtaga gctgggtcta aggggtttaa aaataaaaat ttgaaatatt tggcaggaaa
      301 gcccctagtc catttcgctt tagcaatgat agatatctat caacaaaaac atcctgaaca
      361 taattgcacg acttgtttga atacggacag taatgagtta gtagaattga cgactaagtt
      421 aaatccgtct gtgaaaattg tagaacgtaa acctgaatta gctacagata ctgtagggaa
      481 aattgaagtg atacgtgatt cattcttaca actagaaaga gaagataata tatttgattt
      541 aattatagat ttagatttga cttcaccttt acgtgaagtt tctaatcttg aagaattaat
      601 agatgaacat ctaaaaaatt tagataaaga cgtct
//
LOCUS       DAYYCY010000346          635 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.356, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000346 DAYYCY010000000
VERSION     DAYYCY010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.356"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..270)
                     /locus_tag="ACHAZB_002878"
     CDS             complement(<1..270)
                     /locus_tag="ACHAZB_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8615465.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFR"
     gene            complement(276..>635)
                     /locus_tag="ACHAZB_002879"
     CDS             complement(276..>635)
                     /locus_tag="ACHAZB_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFM8615466.1"
                     /translation="RNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPP
                     RIDTKKLNMLETILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQC
                     ALMERAIAAKRKELDKK"
BASE COUNT          164 a          135 c          110 g          226 t
ORIGIN      
        1 acggaagtag aagaattcct tccagaagtc cgttggcatg cctgtatcga taaaaatatc
       61 gcctatcagc gcatagatga agtttcgttg ctgtacggtg aaacgtctag gatcaataaa
      121 acgaatttca atgactcgat caccatcgta gccgtcatac atcgtcttta gtcgatcaat
      181 gttcacttcc tcattgatgg ttgcgcttat gtcttttcct ttgaactttt tcagaaccgc
      241 tgaatatgaa tcgattaatg gtttaaacac tcatatcact tcttatctaa ttcttttctc
      301 ttagctgcta ttgctcgctc catcaaggca cattgctcat agcttaactg ttcaatagtt
      361 tcaacgttat cagctaagag ccctaattta tctgtctgct cattaacata ttcgattaag
      421 gttttggtca tatctttacc catctgctca ttgaaagctt ctagaatcgt ctctagcatg
      481 tttaatttct ttgtatcgat tctaggtggt gttggaatat cttccccttg aaatacatat
      541 aatcccagtc cgtgtagagc caatgctttc acaaagcatc gcttcaatga gttattgatt
      601 tgcattgcat ttggtttaac aactggttgg tttcg
//
LOCUS       DAYYCY010000347          632 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.357, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000347 DAYYCY010000000
VERSION     DAYYCY010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..632
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.357"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            164..>632
                     /locus_tag="ACHAZB_002880"
     CDS             164..>632
                     /locus_tag="ACHAZB_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFM8615467.1"
                     /translation="MKKTIFVLAIFICFGLLLVGYLLFNSNEMRTISTNDLLKKSELK
                     DKKKESLAFIMFTQEECEYCIAINPIIDSVNKETLNKIKKYDISVDFQSKQKCKIKFV
                     PTIVAYSKNKEVGRIDNELDNLYRKKQDGSIVIDKKKTKTLLIDWMKKMDKQGE"
BASE COUNT          255 a           49 c          118 g          210 t
ORIGIN      
        1 tcgaaaagga atttgtttta aggaaaaatg attctatgga cggttattga tttatattta
       61 ccaaatggct aataaaatag tatttcttat attgtatagt ttttgttttt atgtgaaaac
      121 atgagcaata gaattgtact agtaggataa ggatggatgg aaaatgaaaa aaactatttt
      181 tgttttagcg atttttatat gttttgggct acttttggta ggctacttgt tgtttaattc
      241 taatgaaatg agaactattt ctacgaatga cttgttaaaa aaaagtgaat taaaggataa
      301 aaaaaaagaa tctcttgcct ttataatgtt tactcaagaa gagtgtgagt attgtatagc
      361 aataaatcca atcatagata gtgtaaataa ggaaactttg aataagataa agaaatatga
      421 tatatctgta gattttcaat ctaaacaaaa gtgtaaaatt aagtttgttc cgactattgt
      481 tgcatatagt aagaataaag aagtggggag aattgacaat gaattagata atttgtatcg
      541 aaaaaagcaa gatggttcaa ttgtaataga caagaaaaaa acaaagacat tgcttataga
      601 ttggatgaaa aaaatggata aacaaggaga at
//
LOCUS       DAYYCY010000348          624 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.358, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000348 DAYYCY010000000
VERSION     DAYYCY010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..624
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.358"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>624
                     /locus_tag="ACHAZB_002881"
     CDS             <1..>624
                     /locus_tag="ACHAZB_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFM8615468.1"
                     /translation="DKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYALKI
                     SLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWVPW
                     FYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVPIT
                     LGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPRLG"
BASE COUNT          197 a           78 c          121 g          228 t
ORIGIN      
        1 tgataaatat gttggcagag tagtcggcgg aacactgtca tttgtgttga taggtttagt
       61 aatttattta agaataatga taaagggtgc tcattttttt gataaaaaac agtggagata
      121 cgctttaaaa atctctttgc cgctaatccc acattcctta gcaaatatag ttttagcaca
      181 attcgataga attatgatta attcttattt aggtcctgct gcggggggaa tatattccta
      241 cattagtaac ataggtatta ttctaagcgt aatatggatg tctactaata acgcctgggt
      301 tccatggttc tatggtgaga tgagtaaaaa taatgaaaaa aaagtgaaga aagttagtaa
      361 ctattattta gtagtcttta ctttgattac gatgattgta atggtagttt cgattgattt
      421 agcaaaattt atggctccga aagactatta ttctgggtta tctttagtgg ttccgataac
      481 cttaggctat ttctttcagt ttttatatag tttaccagtt aatgctgaat tttatgagaa
      541 aaaaacatcg tttattgcga ttggtacagt agcaagtgct atagttaata tactattaaa
      601 catgattttc atacctaggt tagg
//
LOCUS       DAYYCY010000349          547 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.361, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000349 DAYYCY010000000
VERSION     DAYYCY010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..547
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.361"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..>547
                     /locus_tag="ACHAZB_002882"
     CDS             6..>547
                     /locus_tag="ACHAZB_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HFM8615469.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     TTIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDI
                     WLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIK
                     YLNNLIEQDHRPVKRRNKFYR"
BASE COUNT          193 a           91 c          107 g          156 t
ORIGIN      
        1 tgacgatgaa tcattttaaa ggaaagcaat ttcagcagga tgtgattatt gtagccgtgg
       61 gctactatct tcgttataac cttagctatc gtgaagttca agaaatctta tatgatcgtg
      121 gcattaacgt ttctcatacg acgatttatc gttgggtgca agaatatggc aaactactct
      181 atcaaatttg gaaaaagaaa aataaaaaat ccttttattc atggaaaatg gatgaaacgt
      241 acatcaaaat taaaggaaaa tggcattatt tgtatcgagc catcgatgca gatggtttaa
      301 ccttggatat ttggttacgt aaaaaacggg acacacaagc agcctatgct tttcttaagc
      361 ggttagtgaa gcagtttgat gaaccgaagg ttgtagtcac agataaagcc ccctctatta
      421 caagtgcctt taagaaacta aaagaatacg gcttttatca agggacagaa catcgtacca
      481 ttaaatacct gaataatttg attgaacaag accatcgtcc agtaaagaga cgcaataaat
      541 tctatcg
//
LOCUS       DAYYCY010000350          535 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.362, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000350 DAYYCY010000000
VERSION     DAYYCY010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..535
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.362"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          209 a           55 c           92 g          179 t
ORIGIN      
        1 ttatagttta gctattttaa aaaatgaaaa ttttttcgtt agtgtattaa tgggtgaaag
       61 tttaatccta atactgattt tgtgtttaat tgttcatgaa gtgaataaac aattgtgaat
      121 taaaaaaata aaaacctagt ataatcactt taataaattg ttggtagaag gtgatcaaaa
      181 tgggagtaaa cgaaatgatg aaagtcagat tgagttttta aatgatacct tcctcagaac
      241 ttcaaaacga attcaaagat tcgttgacaa atcttgagca agatcattta aaaaaatatt
      301 ttctcttcat ttgacgaaaa atgtttagaa atcttatata agtgaagata aagtaattgg
      361 tgtaattaat tgtagagaat agcctttgag caattgaagc gatacaatta tttagaaaat
      421 gaacttcctc cagcatacta catgattcct tacgggacga tgttaaagaa gaaagcaaga
      481 aattttagaa gtattagtag ataaactaat gggcttttct caatttaagg gagta
//
LOCUS       DAYYCY010000351          532 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.363, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000351 DAYYCY010000000
VERSION     DAYYCY010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..532
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.363"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..516
                     /locus_tag="ACHAZB_002883"
     CDS             <1..516
                     /locus_tag="ACHAZB_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFM8615470.1"
                     /translation="SILEKEIKELEIYSNSYLTNKFEYRYLKNIKNEYLKSLLIAKLY
                     QANNNKICCKFECPSTIIEVPVPIFDCIRVLGIILDNAIEASIESQDRELSLAIYRDD
                     KQIEISISNSCREILLSIDTLMKKGITTKKGHQGLGLSSIEEINKKNPNMFVNYQNDP
                     YQFTTQVILLF"
BASE COUNT          208 a           63 c           93 g          168 t
ORIGIN      
        1 tcgatattgg aaaaagaaat aaaagaatta gagatctatt ctaattctta tctaaccaat
       61 aagtttgaat atcgttattt aaaaaatata aaaaatgaat atttgaaaag tttgttgata
      121 gcgaagctat accaagcaaa taacaataag atttgttgta aattcgaatg tccaagtact
      181 attattgaag ttcctgttcc aatatttgac tgtattaggg tgcttggaat tattctcgat
      241 aatgcaattg aagcatctat agagagtcaa gatagagaac tgtctctagc tatttaccga
      301 gatgataaac agatagaaat ttcgatatcg aatagttgta gagagatact actctctata
      361 gatactctaa tgaaaaaagg aataacaaca aaaaagggac atcaagggtt aggtttatct
      421 agtatagagg aaatcaataa aaagaatccc aatatgtttg tgaactatca aaacgatccg
      481 tatcaattta ctactcaagt tattttgttg ttttgaggga aggaggattt at
//
LOCUS       DAYYCY010000352          526 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.365, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000352 DAYYCY010000000
VERSION     DAYYCY010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.365"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(6..359)
                     /gene="tnpB"
                     /locus_tag="ACHAZB_002884"
     CDS             complement(6..359)
                     /gene="tnpB"
                     /locus_tag="ACHAZB_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFM8615471.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
BASE COUNT          153 a          116 c           90 g          167 t
ORIGIN      
        1 ccagcttaaa aaactccttt ttttcctggc aaaatagctc gaggttgatc gatacttaat
       61 ccttctaaca accatcgaac ctgttggttc gttaaggttc gaacttcgtc tttcgtccga
      121 ggccatttta acttgccgtt ttctatccgc ttataacaga gaagaaaacc atcaccgtcc
      181 caatagagaa gtttgaaacg gtcttgacgg ttcccgcaaa ataaaaaaac ggcatcttcg
      241 taaagctcga gttgatactc ttgttgaata agactggcaa gaccatcaat tccttttcgc
      301 aaatctgttt ttccgcacac aatataaata tgtttggttt gaagtaaatc ataaagcatt
      361 ggacaacagt tctttcaaaa tagcttgaag aatataattg ttaataccgt tatagatcaa
      421 aacttcagaa ccattctctc ttttgatgcg catcgctaac ttggcagccg cgggtttacc
      481 tttttctggt gcactctttt cgtttcctaa ttggacagga acaatc
//
LOCUS       DAYYCY010000353          524 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.366, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000353 DAYYCY010000000
VERSION     DAYYCY010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..524
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.366"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..524)
                     /locus_tag="ACHAZB_002885"
                     /pseudo
     CDS             complement(1..524)
                     /locus_tag="ACHAZB_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          187 a           77 c           87 g          173 t
ORIGIN      
        1 ttgcggatct tcatcataaa attctgtagc agtacttgaa aagatgttcc aataaatatc
       61 taatgctgca tattgaattt gtacctcttt tccagaaaaa gactttctgc cattattaga
      121 aaaggagata gacaaaccgt actatgctaa aaatgcatta atctttttca ttttcgtata
      181 caaataagag gcactatagt ttgtttgctg actgaaactg ataatcgaat ttgtctgaca
      241 gctgaacaaa gaagtcaaga tctgataact gactgattcg ttgcagtatc ttccaaatag
      301 acgataatag cagtaagccg gatcaattcc ttcaaaattc aactttaaaa aaggtttttg
      361 cttatcaata tgcagttctg ttagattgag acgctcaaag tcttgttcta attctttgat
      421 gtatagtaaa aaatttgatt cagacatatg gaaatgatct gctaaatatt gtgtagtaat
      481 aaagaaacga tgttcagata ggaaaagaaa aatatctaat tttg
//
LOCUS       DAYYCY010000354          520 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.367, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000354 DAYYCY010000000
VERSION     DAYYCY010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..520
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.367"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>520
                     /locus_tag="ACHAZB_002886"
     CDS             <1..>520
                     /locus_tag="ACHAZB_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8615472.1"
                     /translation="IPLVTAGSRTALQLSDIFNPMIDIYKEKKYIFFTSVYDFEGGEP
                     VGEYELVCKIADLVGKDNLIVKTHPRDVRTIYKDNGFIEDKNSSIPWEAIQLSGDFND
                     KIFMTINSGCVISGSTMSAKPVRTYYMYKLCNIEGNPSCKKNAYDIERLLKQKTMKNI
                     LKNVKIAESLDDI"
BASE COUNT          198 a           64 c          100 g          158 t
ORIGIN      
        1 attccattag ttacagctgg gtcaagaacc gcattgcaac tttcggatat ttttaaccct
       61 atgatagata tttataaaga aaaaaaatat atatttttta cgtctgttta tgattttgag
      121 ggcggagaac ctgtcggaga atatgaatta gtatgcaaaa tagcagactt agtaggaaaa
      181 gataatctaa ttgttaagac gcatcctaga gatgtaagaa caatttataa agataatgga
      241 tttatcgagg ataaaaattc atcaatacct tgggaggcga ttcaattgtc aggcgatttt
      301 aacgataaaa tatttatgac aattaattca ggatgtgtta tttcaggtag tacaatgtct
      361 gcaaaacctg tacgcacgta ttatatgtat aaattgtgta acatagaagg aaatccgtct
      421 tgcaaaaaga atgcgtatga tattgagcga ttgcttaagc aaaaaacgat gaaaaacatt
      481 ttaaagaatg tgaaaatagc agaaagctta gatgatatac
//
LOCUS       DAYYCY010000355          519 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.368, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000355 DAYYCY010000000
VERSION     DAYYCY010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..519
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.368"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>519
                     /locus_tag="ACHAZB_002887"
     CDS             <1..>519
                     /locus_tag="ACHAZB_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8615473.1"
                     /translation="LCMLIDDFRSIITSMVMTYMFSFEGIKGYGYVVFQIMLDSLFLL
                     MFIWLYRKLAINRIVEQSSSKLTAIFMIYLLTIGLFVSYVAHQYQVFDHFVFGVLIFL
                     IIQMFVVLFLFIRITIRQREQYEQQLKRKELVYLKKYTDSLEKDQEKFARFRHDYKNL
                     LLSLKEIANQNHD"
BASE COUNT          172 a           55 c           82 g          210 t
ORIGIN      
        1 ttatgtatgc ttattgatga tttcagatct attattactt ccatggttat gacgtatatg
       61 ttttcatttg aagggataaa agggtatggt tatgtagtat tccagataat gcttgattct
      121 ttgtttttgc tgatgtttat ttggttatat agaaaattag ctataaatag gattgttgaa
      181 cagtccagtt ctaaacttac tgctatattc atgatttatt tacttacaat tggtttattt
      241 gtttcttatg ttgctcatca atatcaagta tttgatcatt ttgtgtttgg tgtcttaata
      301 tttttaatca ttcaaatgtt tgttgttcta tttttattta ttagaataac tattaggcaa
      361 cgagaacaat atgaacagca attgaaaaga aaagaattgg tttatctaaa aaaatatact
      421 gattcactgg aaaaagatca agaaaaattt gccagattta gacatgacta taaaaattta
      481 ttgctgagtt taaaagaaat cgctaatcaa aaccatgat
//
LOCUS       DAYYCY010000356          514 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.369, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000356 DAYYCY010000000
VERSION     DAYYCY010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.369"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..132)
                     /locus_tag="ACHAZB_002888"
     CDS             complement(<1..132)
                     /locus_tag="ACHAZB_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFM8615474.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          186 a           94 c           92 g          142 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag acaa
//
LOCUS       DAYYCY010000357          503 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.371, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000357 DAYYCY010000000
VERSION     DAYYCY010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..503
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.371"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(275..466)
                     /locus_tag="ACHAZB_002889"
     CDS             complement(275..466)
                     /locus_tag="ACHAZB_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8615475.1"
                     /translation="MKQKFFIAIIATKKFHRGKRFGQKWKFPPKVTAPQLKSIKELEF
                     ENALVICMTCTDEVFRKHF"
BASE COUNT          152 a          104 c           75 g          172 t
ORIGIN      
        1 gaaccggggg ctttattcct gcagtccggc tacgccttcc tgcaatagac aaaactccag
       61 atcgggtaat tcgcttgtgg cgaaacccga cctggagttt gtctctgtat tttgaaccct
      121 aaaaattaat caaacagact gatattctca taatcagcgc gaaataagcc attaagtaca
      181 gttttcctac ctatttttga taacccgctg gttagttaat attccttttc cgagagagat
      241 aaatgattgg ttaaatctat atacatctca acctttaaaa atgttttcga aatacttcat
      301 cagtacatgt catacaaatt accagtgcat tctcaaattc tagttcttta attgatttca
      361 gttgaggagc agttactttt ggcggaaatt tccatttttg tccaaacctc ttcccccgat
      421 gaaatttctt tgttgcaatt attgcaatga aaaatttttg tttcaaaaat atacataatt
      481 acttcctcca ataaaaacta tct
//
LOCUS       DAYYCY010000358          496 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.372, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000358 DAYYCY010000000
VERSION     DAYYCY010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..496
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.372"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            110..466
                     /locus_tag="ACHAZB_002890"
     CDS             110..466
                     /locus_tag="ACHAZB_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFM8615476.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
BASE COUNT          194 a           74 c          100 g          128 t
ORIGIN      
        1 tgaagcaata agaacaaaat gaggaaaagt ttaaaattca gctgttgcca acaactttca
       61 atagacgaaa tcttctattt cgttaataat aaggaaagag gtgaagtaca tgaaaaaatt
      121 aattggcgta cttgtcgttt taggcatatt tctcggcgga agttttgcag cttatcatta
      181 tttttacggc ggcgaagctt attatacaaa aatcacgacg aatggcgaaa tgtcagctac
      241 ccaagcagat aacggagaga aattcacgac ttatacctac aagcaagcgg cttataataa
      301 agatggtgaa aaaaaacagg tgacgcttcg cgaagaaaga gaacatccat taaagatgaa
      361 tgcgtatctt aaactcaaag tcaatcctcg taaaggtgta ttaagctggg aagaaataaa
      421 ggcttctgaa gtgcctaaaa atgcggcaga aaaaatcgat aattagtaaa tggtcttaaa
      481 attttattag ataaaa
//
LOCUS       DAYYCY010000359          485 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.374, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000359 DAYYCY010000000
VERSION     DAYYCY010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.374"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(62..259)
                     /locus_tag="ACHAZB_002891"
     CDS             complement(62..259)
                     /locus_tag="ACHAZB_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8615477.1"
                     /translation="MNNGTVKWFNSDKGFGFITGEDGNDVFAHFSAIQGEGFKSLDEG
                     QAVTYDIEEGQRGPQAVNIVK"
BASE COUNT          158 a           89 c           68 g          170 t
ORIGIN      
        1 gaattttttc taccgacata aacaaaaaaa ccacctcata tgaggcggtt aacgttcaac
       61 attattttac gatatttact gcttgaggac cacgttgtcc ttcttcaata tcataagtaa
      121 ctgcttggcc ttcatctaaa gacttaaagc cttctccttg gattgctgag aaatgagcga
      181 atacgtcatt tccatcttca ccagtgatga aaccaaaacc tttatctgag ttaaaccatt
      241 ttactgtacc gttattcata tatatttcct cctgatacgt atataatacg tgttttattg
      301 caattaatat tacttggtaa aaggagtttt tatagtgtga atatatcgct cagattacgt
      361 ttcaaattag attacttatt tactataaca cggatgtaat gctaacacaa ggataatcac
      421 tcgatatacg gtttaaatat tattatttgt caaattaagc gagacaaaac tttccattta
      481 cgtaa
//
LOCUS       DAYYCY010000360          483 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.375, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000360 DAYYCY010000000
VERSION     DAYYCY010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..483
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.375"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>483
                     /locus_tag="ACHAZB_002892"
     CDS             <1..>483
                     /locus_tag="ACHAZB_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8615478.1"
                     /translation="YQYHVGQYLPDWHPWEDYRNFFVSNKKTNGCREIFAIELPWIID
                     TFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETGIVGTLSVDVVSRVAKRNLTIIGE
                     KILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHDNNYSHTIIEDAYLEEIKEFFTLL
                     E"
BASE COUNT          166 a           59 c           91 g          167 t
ORIGIN      
        1 tatcaatacc atgtaggaca gtatttaccc gactggcatc cttgggaaga ttatcgcaat
       61 ttttttgttt caaacaaaaa aactaatggg tgtagagaaa tattcgctat tgaattacct
      121 tggattatag atacatttgg gaaagttaaa tcgtttactg taatgaccca gaaattatcc
      181 aatcttgata ttgattattc ggacagtttc actcttatta ttgagcatga aactggtata
      241 gttggaactt taagtgtaga tgttgtttca agagtagcta aaagaaattt aactataatt
      301 ggtgaaaaga ttttaataga atgggaaggt aacccggatt ctctattttc atggaatgaa
      361 gaaacttcta gtatgacaga tattcaattg tacgatgagg ttatgcatga taataattat
      421 tcacatacta ttattgaaga tgcatattta gaggagatta aagaattttt cacgcttttg
      481 gaa
//
LOCUS       DAYYCY010000361          477 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.376, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000361 DAYYCY010000000
VERSION     DAYYCY010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..477
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.376"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>477
                     /locus_tag="ACHAZB_002893"
     CDS             <1..>477
                     /locus_tag="ACHAZB_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFM8615479.1"
                     /translation="FTAGEGTSIESGTTVFAVKDGVSLPEDKLPVLKAKDGYTDAKWP
                     EEATQPITADDTEFVSSATKLDDKSDADKYNPEGQKVTTELNKEPDAFEGIKNKEDLP
                     KDTKYTWKEKVDVSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEG"
BASE COUNT          192 a           73 c          112 g          100 t
ORIGIN      
        1 cttcaccgca ggagaaggaa caagtattga aagtggaaca acagtctttg cagtgaaaga
       61 cggcgtaagc ttaccagaag ataaacttcc agtgttgaaa gcaaaagatg gttatacaga
      121 tgcgaaatgg ccagaggaag caactcaacc aattacagca gatgatacag aatttgtatc
      181 aagtgcaaca aaactggatg ataaatctga cgctgacaaa tataatcctg aaggtcaaaa
      241 agtgactaca gaattgaata aggaaccaga cgcatttgag ggaattaaga acaaagaaga
      301 tttaccaaaa gatactaagt atacttggaa agaaaaagta gatgttagcg cagctggaaa
      361 taaaaaaggg actgttgtag tgacatatcc agatggatca tctgatgaag ttgaagtaga
      421 tgttacagtg acagacaatc gttctgacgc cgataaatat gagccaacag tagaagg
//
LOCUS       DAYYCY010000362          468 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.378, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000362 DAYYCY010000000
VERSION     DAYYCY010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.378"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..>468)
                     /locus_tag="ACHAZB_002894"
     CDS             complement(1..>468)
                     /locus_tag="ACHAZB_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8615480.1"
                     /translation="IICLHHLFLSKTIPRYSKNKKAEKFCLLLNKFTLIAPILAFIIF
                     SVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFLMSLKPPKSISLCLVINGIFLLS
                     LIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYIGYFPNKNNNFYFYRNKL"
BASE COUNT          198 a           53 c           71 g          146 t
ORIGIN      
        1 ttacaattta ttgcgataaa aataaaagtt gttattttta ttgggaaaat acccgatgta
       61 gaaaataatg ccttccaata tacctataaa ataagtccag tattccaaag gattatataa
      121 ataagtgacg tatctatcta aaggtgtaat aaaaatgata agtgataata aaaaaatacc
      181 atttataact aaacataaac taatggattt gggcggtttc aggctcatca gaaaaatata
      241 gattctggtg aacaacaacc ataaaaaagt gagaatcatt gcatgagagc ttctttccat
      301 aaattttcct ttaagagtag tgcttaaaag tacagaaaaa ataataaatg caaggatagg
      361 agcaattaaa gtaaatttat ttaataataa acaaaatttt tcagcttttt tattcttgga
      421 ataacgaggt attgttttcg ataaaaaaag atggtgcaga caaattat
//
LOCUS       DAYYCY010000363          452 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.380, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000363 DAYYCY010000000
VERSION     DAYYCY010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.380"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..452
                     /locus_tag="ACHAZB_002895"
     CDS             <1..452
                     /locus_tag="ACHAZB_002895"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005225824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HFM8615481.1"
                     /translation="TTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQLEELYQAY
                     QTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTDPLFTYLK
                     NEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          161 a           90 c           71 g          130 t
ORIGIN      
        1 aaactacttt agaagacggc actgtctatt cattgaaaaa atatgcaggc aaaccgataa
       61 tcattgttaa cactgcaaca aaatgcggtc tagcacctca atttaaacaa ttagaagaac
      121 tttaccaagc ttatcagaca caaggtctgg ttatcttagg ctttccttcc aatcaattta
      181 aacaagagct agattcagcg actgaagcag cagaagcttg taaaaccact tatggcgtta
      241 ccttccctat gcatcaattg attgacgtaa atggagaaaa tacagatcct ctttttactt
      301 atcttaaaaa tgaagctcct ggtactgtca ctaaagcaat aaaatggaat tttactaaat
      361 ttttgattga tagaaatggc aaagtaatcg aacgatttgc accacaaact tctccattga
      421 aaatgaccgc tgcaattgaa tccgttcttt aa
//
LOCUS       DAYYCY010000364          452 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.381, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000364 DAYYCY010000000
VERSION     DAYYCY010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.381"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>452
                     /locus_tag="ACHAZB_002896"
     CDS             <1..>452
                     /locus_tag="ACHAZB_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8615482.1"
                     /translation="GKPNLMSKLKSSDFNVGYHGSLTSDNEKLHFTSDGTGSIIMFTR
                     INTPQLASGKTYTLSAKVRFDEGTTGAIDKLRLVYRTSPGEKILLEANSTNITTDDVG
                     KEITIKGTANVNYQITNLDRFYMSISFVDRDKINGGFKLYDIKIEEGST"
BASE COUNT          165 a           68 c           92 g          127 t
ORIGIN      
        1 ggtaagccga atttaatgtc caaactaaaa tcgagcgatt ttaacgttgg ttaccacggg
       61 tcactaactt cggataacga aaagctacat tttacttctg atggtacagg aagcattatt
      121 atgtttacgc gtattaatac acctcagctt gctagtggga aaacctatac tctgagtgcg
      181 aaagttcgat ttgatgaagg aactacagga gctattgata aattacgttt ggtgtatcgt
      241 acatcaccag gagaaaagat attattggaa gcaaatagta caaatattac aacagatgat
      301 gtagggaaag aaataacaat caaaggtaca gctaacgtta attatcaaat cacaaattta
      361 gatcgatttt atatgagtat tagctttgtt gacagggata aaataaatgg cggatttaag
      421 ttgtacgaca tcaaaatcga agaaggctca ac
//
LOCUS       DAYYCY010000365          431 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.383, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000365 DAYYCY010000000
VERSION     DAYYCY010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..431
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.383"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          131 a           86 c           52 g          162 t
ORIGIN      
        1 gttattaaaa aaacagatga aagatcaggt tttattagat ctcattcttt catcacttga
       61 agggatttaa tgaactactt tttcctcttt gtcgttttta tatattcgta ttccttcaaa
      121 cagtaggata gcctctttca atgcttgata tttgaactgt ccgttactta aaaatttaga
      181 tgtgccgccc tcttcctaga acatattctt ttttactttt tcattaattg acagaccaat
      241 cgaacgaaac tgtaaatttc gataggataa tgattggtca aactcattca ttttcaagac
      301 atctcgattc atattcaaga taggaatctt tttatcaaca tcactttatt cgtattttcc
      361 tctaccataa aagcttcacg ctccacaaaa ttgttttata cactactatt cagcccattc
      421 aacgaccaat g
//
LOCUS       DAYYCY010000366          423 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.384, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000366 DAYYCY010000000
VERSION     DAYYCY010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..423
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.384"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            2..423
                     /locus_tag="ACHAZB_002897"
                     /pseudo
     CDS             2..423
                     /locus_tag="ACHAZB_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612034.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          171 a           75 c           81 g           96 t
ORIGIN      
        1 aatcgtcaat ctttacttag ctggaaagcc acgtgtagaa atcattcgag aatatgaact
       61 aacggcttca gcatttgaca aatgggtaaa gcaatctaaa acgagtggtt cattcaaaga
      121 aaaagataat cttacgcctg aacaaaaaga attgttagaa ctacgtaaaa gaaaccagca
      181 attagaaatg gaaaatgata ttttaaagca agcagcgctg atattcggac gaagagacaa
      241 gtaatcgatg cgaataagca tctttaccct atatcagcga tgtgcagaat attaggtcta
      301 tcacgtcagt cctattatta tcaatcaaaa ccaaagaaag acgaatcaga acttgaagaa
      361 gtagtcgctg aagaatttat ccgcagccga aaggcctacg gctcaagaaa aataaaaaaa
      421 gcc
//
LOCUS       DAYYCY010000367          417 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.385, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000367 DAYYCY010000000
VERSION     DAYYCY010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.385"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>417
                     /locus_tag="ACHAZB_002898"
     CDS             <1..>417
                     /locus_tag="ACHAZB_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8615483.1"
                     /translation="IKKIPKIDENSKLMKNIQSIFGIEEVFVGDKCIFFDQPLLSDGY
                     GIDETDIFAKVNSIFSGKKETLIKLHPRARTNRYGEKNILNSSLPFEVLLLENDFSST
                     LLISPISTISFSPSMMFEKTINSIMLAKLILNEYTA"
BASE COUNT          155 a           57 c           60 g          145 t
ORIGIN      
        1 tataaaaaaa attcccaaaa tagatgaaaa cagtaaattg atgaaaaaca tacaatcaat
       61 ctttgggata gaagaagttt ttgtcggaga taaatgtatc ttttttgatc aacctttatt
      121 atctgatggt tatggcatag atgaaacaga tattttcgct aaagtaaata gtatattttc
      181 tggaaaaaaa gaaactttaa ttaagcttca tcctagagca aggacaaatc gttatggtga
      241 aaaaaacata ttgaattcca gtttaccgtt tgaagtcttg ctacttgaaa atgacttctc
      301 ttcaacactt ttgattagtc ctatatctac aatttctttt tcaccatcta tgatgtttga
      361 aaagacgatc aactctatta tgttggctaa attaatttta aatgaatata cagcatt
//
LOCUS       DAYYCY010000368          399 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.386, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000368 DAYYCY010000000
VERSION     DAYYCY010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.386"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          111 a           98 c           76 g          114 t
ORIGIN      
        1 tataaataac tcataaaaaa tattttgact ttcaaaatca cttttgctac cagaagttga
       61 ggcgaagtgt tttaagcata gttgtaccct agtttgaccg ttaaaaataa ataacgatcg
      121 ctagagtgca actatgtttg tttgccctac cgtcagcgtc ggcttggcct ctcacatcca
      181 ttaccgtcgg gattatctcc gttacatacc cttacaaaat acgccctgta agcattgcat
      241 gaaaccaccg tgcatttgca ccgtatttcc gccctagccg ttcaaatcgg tcgctaccac
      301 cattcagaaa cacgtgcggt aatggaaggt ttggaggaag ctatccagac cactaacgat
      361 tgtccctatc gtcgtacgtt tcactcagat caaggttgg
//
LOCUS       DAYYCY010000369          386 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.388, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000369 DAYYCY010000000
VERSION     DAYYCY010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.388"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(160..>386)
                     /locus_tag="ACHAZB_002899"
     CDS             complement(160..>386)
                     /locus_tag="ACHAZB_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8615484.1"
                     /translation="FTEEYQVDYYEVIDKCVHFVYEKVWNFMNFAISEEILRVQENRT
                     GIFTRDRKLNLIAHYIRNRWSSIPNFNYKK"
BASE COUNT          128 a           61 c           41 g          156 t
ORIGIN      
        1 tttactatca aaaaaacaaa aaaaacgcgt ttctacgcgt gaccaaaatt tttagctcta
       61 taatttctaa gactgataga aaatcattct gtttttgaag tcttttttca taaatactac
      121 ttgatttatt gtgtatattg tataaaagat tgttcataat tattttttat aattaaaatt
      181 tggtattgat gaccagcgat tccgtatata atgcgcaatc aaattaagct ttctgtctct
      241 ggtaaatatt cctgttctgt tttcttgtac tcgtaagatt tcttcactta tcgcaaaatt
      301 cataaaattc cacacttttt cataaacaaa atgtacacat ttatctatta cttcataata
      361 gtctacttga tattcttctg taaaca
//
LOCUS       DAYYCY010000370          364 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.392, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000370 DAYYCY010000000
VERSION     DAYYCY010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.392"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          134 a           53 c           46 g          131 t
ORIGIN      
        1 tttaggtcta tttaactcat cagacattct atgctcattt ttctccaaat ttgtttcaac
       61 tctttcgtac ttaattttgc gtatttgaaa attcttcttt tagagtactt ttattacgat
      121 actagtatga tatatatcta tagcaaacag ttaaaatctt cctaaaataa gcgaatagca
      181 ttcctaaaac ctataaaatt tttagatgaa gcgacaagtg gacaattaat aaactacttg
      241 aacatgaaat tatggatcac ctttcaaatt tagaaaatgg aattcttata cttattgcgc
      301 aacatttatt gattgcaaaa ttatgtgaca aaaaaaacta gtagaagaaa gaataatggc
      361 ttga
//
LOCUS       DAYYCY010000371          358 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.393, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000371 DAYYCY010000000
VERSION     DAYYCY010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..358
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.393"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          114 a           55 c           59 g          130 t
ORIGIN      
        1 aaaaacaagg cgagactgat tgatgtctcg ccatttttgt ttccattcta ttgacagctg
       61 ctttcttact ttgtatcttt ttaaaaacgt gtgatatttt ttccgcgagt tttttcaacg
      121 caaggtatat cttgtcataa ttcgcctttc caaaaaaatt caatacataa taagttatat
      181 ccgtttcttt ggggataaga agcaaaaaag gaaagacgta ttattgcttc aaaaactttt
      241 gccattgatt atgttgtaga gacatttggt tatatttgtt caatcatcgg tatcttaacg
      301 gctacgattg tagtaggaaa atctacttat tttatcaagt aagaaaaata gaaaacgc
//
LOCUS       DAYYCY010000372          331 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.395, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000372 DAYYCY010000000
VERSION     DAYYCY010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..331
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.395"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>331
                     /locus_tag="ACHAZB_002900"
     CDS             <1..>331
                     /locus_tag="ACHAZB_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS6-like element ISS1D family transposase"
                     /protein_id="HFM8615485.1"
                     /translation="YLWKKKNRQSFYSWKMDETYIKIKGRWHYLYRAIDADGLTLDIW
                     LRKKRDTQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQSVGLYTKTEHRTVKY
                     LNNLIEQ"
BASE COUNT          114 a           60 c           68 g           89 t
ORIGIN      
        1 attatctttg gaagaagaaa aatagacaat ccttctattc atggaaaatg gacgaaacct
       61 atatcaaaat taagggacgt tggcattatc tttatcgtgc aattgatgcg gacggcttaa
      121 ccttagatat ctggttacga aagaaacggg atacgcaagc agcctatgct ttcttaaaac
      181 gactccataa acagtttggt gagccgaaag caattgtgac cgataaagca ccttctcttg
      241 gctccgcctt tagaaagtta cagagtgtgg gtttatatac taagacagag caccgaactg
      301 tgaagtatct taacaattta atagaacaag a
//
LOCUS       DAYYCY010000373          321 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.396, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000373 DAYYCY010000000
VERSION     DAYYCY010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.396"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>321)
                     /locus_tag="ACHAZB_002901"
     CDS             complement(<1..>321)
                     /locus_tag="ACHAZB_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8615486.1"
                     /translation="ITRHVWGDLMDEVEHLRLTDLNKSIYKKRKQTIERIFADAKEKH
                     GMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQAR
                     VTSL"
BASE COUNT           79 a           78 c           51 g          113 t
ORIGIN      
        1 tccaaactcg taactcgagc ttggaacagc tttttatcta cttcgtttcg aatttttgaa
       61 caaaaaaata aaggctcctt gcctttccat agccaggtgg ctaatttctt taaattcatt
      121 gcagcaaaca ctagcatcgt atgcgtcgcg actttttcca agcctcgata cttggtccaa
      181 cgcatcccat gcttttcttt tgcatccgca aatattcgtt caatcgtttg tttccgtttt
      241 ttgtaaattg atttattcag atctgttagg cgaagatgtt ccacttcatc catcagatct
      301 ccccaaacat gtctcgtgat g
//
LOCUS       DAYYCY010000374          312 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.398, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000374 DAYYCY010000000
VERSION     DAYYCY010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..312
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.398"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>312
                     /locus_tag="ACHAZB_002902"
     CDS             <1..>312
                     /locus_tag="ACHAZB_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM8615487.1"
                     /translation="GDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQW
                     FSRFPKNFFKSITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRN
                     GL"
BASE COUNT           97 a           63 c           55 g           97 t
ORIGIN      
        1 ggtgatacga tcgttggcat tcatcataaa agtgccgtca ttactttagt tgaaagatta
       61 tctaaagtca ttatcacgat taaacccaac ggccgtaagg cattagatat tgaaactgcc
      121 cttaatcaat ggttttctcg cttccctaaa aacttcttta aatctattac gtttgactgt
      181 ggaaaagaat tttctaactg gaaagccatt agtaaccaac atgatattga tatatatttt
      241 gcggaccctg gaacaccttc tcaacgccca ttaaacgaga attctaacgg gattctgcgt
      301 cgtaatggac tg
//
LOCUS       DAYYCY010000375          308 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.400, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000375 DAYYCY010000000
VERSION     DAYYCY010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.400"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(76..>308)
                     /locus_tag="ACHAZB_002903"
     CDS             complement(76..>308)
                     /locus_tag="ACHAZB_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8615488.1"
                     /translation="LKNLPETLDAQLRTKLQNLLTYEEGIYNAMIYPYSNGKIEAKIP
                     HIKTLKRLSYGFKSFENMKIRIFLINQLIQVK"
BASE COUNT           94 a           46 c           56 g          112 t
ORIGIN      
        1 atgttggtga agttcaccaa catcaaaaag tatagagccg atagaaggac tgaccttctg
       61 tctcagattt ttttgttatt tcacttgaat caattgattg atcaaaaaaa tcctgatttt
      121 catgttctca aatgatttaa atccataaga taatcgtttc agtgtcttga tatggggaat
      181 cttggcctct attttgccat tagaatacgg gtaaatcata gcattgtaaa taccttcttc
      241 atacgttaaa aggttttgaa gtttcgtgcg aagttgtgca tcaagtgttt caggaagatt
      301 ttttaata
//
LOCUS       DAYYCY010000376          307 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.401, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000376 DAYYCY010000000
VERSION     DAYYCY010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..307
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.401"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="ACHAZB_002904"
     CDS             complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="ACHAZB_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HFM8615489.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLW"
BASE COUNT           96 a           45 c           50 g          116 t
ORIGIN      
        1 ttccaaagaa attgtttgac tcttggaaag tcacgtttta tcaatcttga gctggcactt
       61 ttataggcat tgatgaattt tgtcaattct gttttaggtt gagctttgaa cataatatga
      121 acgtggtctt tatcatgatt ccactccact aaagtaatgt gatacgattc tgaaattctt
      181 tcaaaagtag ttttggcata atcagatatt tcatcatcaa tcacttgtct acgatatttc
      241 gttactaaaa caaggtggta ataaagaaga aatactgaat gattatttgt atctaattcc
      301 atgttat
//
LOCUS       DAYYCY010000377          300 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.402, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000377 DAYYCY010000000
VERSION     DAYYCY010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..300
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.402"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          121 a           32 c           52 g           95 t
ORIGIN      
        1 gctatattat tatttgttct aaatgattcc tttaaggtgt cactaaaaat tataaaaaat
       61 agtgttgacg cacttttgat gtcatgttat aataacgaag tcgtttggat gagatagcta
      121 ataaagcttg attttatcga aaaaaacttc gaaaaagtta ttgacaaata acaataaaga
      181 tggtatgata atcaagtcgc tgctaatgag tggcaaagac gtttagaaaa aataaacaaa
      241 gaagttgttg acattctctt ctgaatctga taagattaaa gagttgttaa aacaaaaaga
//
LOCUS       DAYYCY010000378          295 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.403, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000378 DAYYCY010000000
VERSION     DAYYCY010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..295
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.403"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..295
                     /locus_tag="ACHAZB_002905"
                     /pseudo
     CDS             1..295
                     /locus_tag="ACHAZB_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          115 a           59 c           58 g           63 t
ORIGIN      
        1 aaacaacaaa tcgtcgatct ctatctcgct ggtaagcctc gcgcagaaat tattcgagag
       61 tatgagctta cgccttcttc tttcgataaa tggatgaagc aagcacaatc aacgggctca
      121 ttcaaagaaa gagacaactt aacaccagaa caagcagaat tgatcgcact aagaaagaaa
      181 aataagcaac tcgaaatgga gaatgatatt ttaaagcaag cggcgctgat attcggacga
      241 aaagacaagt aattgatgcc aacaagcata aatattccat atcagcgatg tgcaa
//
LOCUS       DAYYCY010000379          292 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.404, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000379 DAYYCY010000000
VERSION     DAYYCY010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..292
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.404"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..185
                     /locus_tag="ACHAZB_002906"
     CDS             <1..185
                     /locus_tag="ACHAZB_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM8615490.1"
                     /translation="YTFETLQQLDLELFDYVNWWNHLRLHGTLGYETPVGYRNQRLAQ
                     RILDNELGCANASEAV"
BASE COUNT           83 a           63 c           69 g           77 t
ORIGIN      
        1 aatacacatt tgaaacctta caacaattgg atttggagtt atttgactat gtcaattggt
       61 ggaaccacct tcggttgcac ggtacacttg gctacgagac accggttggt taccgtaacc
      121 agagattggc gcagcgaatc cttgataatg agctcggatg tgctaacgct agcgaggcag
      181 tctaacttta actgttagct cctgccgaag atcgtcacat ccgaggaggc tcattgtcaa
      241 ggacaatcgg aatagcatac ggaaagaagt tctgcacctt ataaaatttg tc
//
LOCUS       DAYYCY010000380          291 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.405, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000380 DAYYCY010000000
VERSION     DAYYCY010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..291
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.405"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>291
                     /locus_tag="ACHAZB_002907"
     CDS             <1..>291
                     /locus_tag="ACHAZB_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFM8615491.1"
                     /translation="LVKQSKRLFPEINCVVADAGYKTPKFVHFLTHLNLRPCLPYSRP
                     KGKKGLLSKNEFLYDEYFDCYICPQDQMLAFSTVTREGYREYKSNPKECVNC"
BASE COUNT           86 a           55 c           58 g           92 t
ORIGIN      
        1 agctcgtcaa acaatcgaag agactttttc ctgaaatcaa ttgtgttgtc gctgatgctg
       61 ggtataagac acctaagttt gttcactttt tgactcattt gaaccttcga ccttgtttgc
      121 cttattcaag gcccaaaggg aaaaaggggc tattatcaaa aaatgagttt ctctatgacg
      181 agtattttga ttgttatatt tgtccgcaag atcaaatgct cgctttttca acggttactc
      241 gagaagggta ccgagaatac aaatccaatc caaaagaatg tgtgaactgt c
//
LOCUS       DAYYCY010000381          276 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.406, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000381 DAYYCY010000000
VERSION     DAYYCY010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..276
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.406"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(34..252)
                     /locus_tag="ACHAZB_002908"
     CDS             complement(34..252)
                     /locus_tag="ACHAZB_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8615492.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
BASE COUNT           95 a           57 c           42 g           82 t
ORIGIN      
        1 cttgtctttt gaataattag cagtcaatcc ttattatgat tgcctggatc tgtagtaggg
       61 ctcatgatag aaatgatctt catgaattct gcgccgctca gattgacggt acttcttttc
      121 atcccacaac atgaaccaca aagtaaataa cggaacaaac acaagtaaag ctggacgtat
      181 gccgaaatta ccaataataa tacctaaaaa caaaacagtt aatactaagc caagacgtct
      241 cattgatttc atgaattctc acctctttaa tttatg
//
LOCUS       DAYYCY010000382          270 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.408, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000382 DAYYCY010000000
VERSION     DAYYCY010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..270
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.408"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>270
                     /locus_tag="ACHAZB_002909"
     CDS             <1..>270
                     /locus_tag="ACHAZB_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM8615493.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGW"
BASE COUNT           83 a           56 c           53 g           78 t
ORIGIN      
        1 atccatcgtc gtttctacac cagtatctgc catcagaaaa tcacgacgga tacaactgaa
       61 tttaaatacc atgaagtgga tgccaaaggc attatccgtc aaaagaaatt gtatctagat
      121 ccttttatgg atatgttcaa tagtgagatt ttatcctatc gtatctctga aaagcccaat
      181 gcattagcgg taatggaagg tttggaggaa gctatccaga ccactaacga ttgtccctat
      241 cgtcgtacgt ttcactcaga tcaaggttgg
//
LOCUS       DAYYCY010000383          239 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.410, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000383 DAYYCY010000000
VERSION     DAYYCY010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..239
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.410"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>239
                     /locus_tag="ACHAZB_002910"
     CDS             <1..>239
                     /locus_tag="ACHAZB_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM8615494.1"
                     /translation="AIIALKPEGRKAVDIENSINEWLQSVPKNLFKSITFDCGKEFSN
                     WKSISNTNDIDIYFADPGTPSQRGLNEHSNGLLRK"
BASE COUNT           84 a           46 c           43 g           66 t
ORIGIN      
        1 gccattatcg ccttgaaacc agaaggccgt aaggcagttg atattgaaaa ttcgattaat
       61 gaatggcttc aatccgtacc caaaaatctt ttcaaatcaa ttacctttga ttgtggaaaa
      121 gaattctcta attggaaaag tattagtaat acgaatgata ttgatattta tttcgcagac
      181 ccaggaacgc cttcccaacg gggattaaat gaacattcaa acggactcct ccgaaaaga
//
LOCUS       DAYYCY010000384          235 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.denovo.411, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000384 DAYYCY010000000
VERSION     DAYYCY010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..235
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.denovo.411"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..223)
                     /locus_tag="ACHAZB_002911"
     CDS             complement(<1..223)
                     /locus_tag="ACHAZB_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFM8615495.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLE"
BASE COUNT           74 a           43 c           21 g           97 t
ORIGIN      
        1 tttccagatg cttaaaagtc ggatttgtaa taaaagctat atcataatgt gtatccaaat
       61 cgtctctatt ttgaatatac gttatttctt caaactcttt ttttccactg ctactataag
      121 ctgttatatc aaaattaata gaattctttt ttccatagct tattagattt tttagatgtc
      181 tctttccaat tgaccctaac ccaaaaaaaa ttactttcat cattaattta cctca
//
LOCUS       DAYYCY010000385         6262 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.guided.139, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000385 DAYYCY010000000
VERSION     DAYYCY010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6262
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.guided.139"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            80..433
                     /locus_tag="ACHAZB_002912"
     CDS             80..433
                     /locus_tag="ACHAZB_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8615496.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            complement(491..679)
                     /locus_tag="ACHAZB_002913"
     CDS             complement(491..679)
                     /locus_tag="ACHAZB_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HFM8615497.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(777..1904)
                     /locus_tag="ACHAZB_002914"
                     /pseudo
     CDS             complement(777..1904)
                     /locus_tag="ACHAZB_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetM/TetW/TetO/TetS family tetracycline
                     resistance ribosomal protection protein"
     gene            2179..2676
                     /gene="dfrG"
                     /locus_tag="ACHAZB_002915"
     CDS             2179..2676
                     /gene="dfrG"
                     /locus_tag="ACHAZB_002915"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFM8615498.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(2748..4700)
                     /locus_tag="ACHAZB_002916"
     CDS             complement(2748..4700)
                     /locus_tag="ACHAZB_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFM8615499.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(4707..4943)
                     /locus_tag="ACHAZB_002917"
     CDS             complement(4707..4943)
                     /locus_tag="ACHAZB_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8615500.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            complement(5181..5981)
                     /locus_tag="ACHAZB_002918"
                     /pseudo
     CDS             complement(5181..5981)
                     /locus_tag="ACHAZB_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
     gene            complement(5997..6044)
                     /locus_tag="ACHAZB_002919"
                     /pseudo
     CDS             complement(5997..6044)
                     /locus_tag="ACHAZB_002919"
                     /inference="COORDINATES: protein motif:HMM:NF011335.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1973 a         1125 c          999 g         2165 t
ORIGIN      
        1 ctcataaact atataacatg tttttctata cctgtttatg gttatatagg aacagtaaaa
       61 tgtatagagg tgatttagta tgcgtaaaaa agaagataaa tacgatttta gagccgttgg
      121 tttagcgatt aaagaggctc gaatgaaacg gggtctcact cgtgaacaag tgggaacaat
      181 gattgaaatt gacccacgtt atttaacaaa tatagaaaac aaaggacaac acccaagtac
      241 acaagtgctg tatgatcttg tatcattact ccatgtatct attgatgaat ttttcttacc
      301 tacagataat ttgattaaaa gcactcgaag gttacaggta gaaaaataca tggatagctt
      361 tacagacaaa gaactatcct taatggaagc actagcaaaa ggtatcaatg aagcaagaaa
      421 tattgaaggc tagtcagtaa aattcagata aacaaaaaga gccgataaga tgagagtttc
      481 atttctcaaa ttatcggctc tgcgtcttgg cgtctggctc tttgattgtt attatattca
      541 ttttttgaac attaattaaa gtttcgttct tacatttggg gcagtataaa gggaaatttt
      601 taagtatagt atccactcgt attcgtagtc ttgttttatt tccacaaata ggacacaata
      661 tccacttgta gtttataata acaatctcct cctttccact ttaattcaaa tctatattaa
      721 agaatatttc atcttattta ataagaaacc atatttatat aacaacataa aacgcactaa
      781 gttattttat tgaacatata tcttacttta tctatccgac tatttagacg acgggtctgg
      841 caaacaggtt cgccagtggt aacctgatat ccttttagct ctgctaaaca aacactaagc
      901 ccatttgtaa aaaaagttaa atcattgcga taatcttgaa tacatcgagc aggaatttct
      961 ccaataataa tgacctcatt atttttcagt tgagtattta cgatatttgc acaatatttg
     1021 ggagcatcgt tatatgcccg tgaaagatat tcctgtggtg cataaacttt aaaactaaga
     1081 tatggctcta acaattctgt tccagctttt ctaaaggctt gctccagtac aataggagta
     1141 agcatccgaa aatctgctgg agtactaaca gggctatagt ataaaccgta cttaaaacag
     1201 attttacaat ccgtcacatt ccaaccatat aatccttgtt cgcaaccata gcgtatccct
     1261 tccataactg cattttgaaa tgattgattt aagtatccaa gagaaaccga gctctcatac
     1321 tgcattccac ttcccaacgg aagcggtgat acagataaac caatggaagc ccagaaagga
     1381 tttggcggca cttcgatgtg aatggtatat tctgcatttt ttaacggtct ctccatataa
     1441 atgactgtag gctcttttag ttctatctcc acatgatact tttcttgcaa cagtgcacta
     1501 atcacttcca tttgtacttt ccctaagaaa gaaagtataa tttcatgtgt cgtagaatcc
     1561 acgtaatatc gtagaagcgg atcactatct gagatttcca aaagggcatc aagcaacatt
     1621 tctctctgtt caggtttact cggttcaaca gttgtttgta gtagagggtg cggattttca
     1681 atcttttttc tctgtggcaa tagttttgta tctccaagaa cactatttaa cttcaaaaac
     1741 tcattttgca aaataacaat ttctccagaa taagctctat caatcttaca taattcacca
     1801 tttattgaag tatacatttc tgtaactttt attttttctt tttctgatac tctaaccgaa
     1861 tctcgtaaat gtagtactcc actataaagg cgtatatatg caagtaagta gttcagtttt
     1921 ggagtacaaa atgacaaaat gtacttcaaa atggactact tttttcattt taacttacat
     1981 accaaattac atacaaaatg atttataaaa tgcaattgaa aatgataatt gaatgaaaat
     2041 ttgtgatttt gtggtattat gataatataa cttatagaaa aaattgctta ttcaactaac
     2101 gggcaggata atgagaaaag gttttttgat agaaataaga aattagatgg taaattagtt
     2161 aaaaaggctg tgttataaat gaaagtttct ttgattgctg cgatggataa gaatagagtg
     2221 attggcaaag agaatgacat tccttggagg attcccaagg actgggaata tgttaaaaat
     2281 actacaaagg gacatccgat aatattaggt aggaagaacc ttgaatcaat cggaagagcc
     2341 ttacctgaca gaagaaatat tattctgacg agagataagg ggtttacctt taatggttgt
     2401 gaaattgttc attcaataga agatgttttt gagttatgta aaaacgaaga agaaattttt
     2461 attttcggag gagaacagat ttataatttg tttttccctt atgttgagaa aatgtacatc
     2521 acaaaaatac atcatgaatt cgaaggagat actttttttc cagaagtgaa ttatgaggaa
     2581 tggaatgagg tatttgccca aaaagggata aagaatgata aaaatccgta taactactat
     2641 tttcatgtat atgaaagaaa aaacttattg agttaacggg tgcgttaatt gaagaagaac
     2701 agtaaagata aagcacaaga gcttgggatt actccaagct ctttttgtca tttgaaataa
     2761 attatatctt tccaatcaaa ttgtttatct gtaatcccta atctttgagc aggtgtcaaa
     2821 acctttttat ccgctgattt aaaagggcga caaaagttgt agtaagttct tagaattgta
     2881 atcgcaaatt gagcatactt cggattgaag ttagcgtaaa tatagctctt cttatcacca
     2941 cgagctgttg ttaatggtct ttcaagagat gatatataac gtcttatctg ttgaataaag
     3001 ctattggtcg aatggtcatt aacatttaat agcatctttg cgatttcgtt tggttccaga
     3061 gcagataaat ccgttctaca atcaactgaa taaaatcctt tatccttagt ggctaatgga
     3121 tgtttgatag ggttatcggc atatttgcga tatctttctc catctttact aatagcctct
     3181 tcgtggaatt gatgacttgt tagtaattca gttagataat gagatgccaa agtccgtaga
     3241 aactttgttt tgaatcctct gatatctccc caatcgagca gttctgcttt tgcttgtccg
     3301 aattctttta agcattgttt tctgcttttc gtcttgttaa cttggcaaat aaaatgatgt
     3361 gcatcgctca accttaattc tttcgtgaat actctataaa aggcgtttct aatagagaag
     3421 tcatcatcac taatcattcg ccattcgtcg gcattaacca tttgtttaat taaccaatag
     3481 tgagcagttg tagtataagg agcatcaaca tttaaaccat cgacaaatct actcctatta
     3541 gtaattttcc ctaattcgtg aagataagca ttcctatttt cgctatcgtt agcggatggt
     3601 tcttgtggat aataagacca atctaaccta tcatttttac gactaaaagt attgagatgg
     3661 tcttctttga attttcttgt atcttcattt aattcatcca ttgaaatatt ccaatcatag
     3721 gcaacatcag aacggaacac atatcttgat aagacctcag cagatacaac tatatacgtt
     3781 tgcatattaa ggtcttcaaa ccccgtatat ttctttgaac cttgtccttt cttacggacg
     3841 ttatttaggt agtagtgcat cttgtccgta ttaagccaca tctcgttaaa tttctttgtt
     3901 tgaaggggtt gtgtctcata tcgttctaaa aactccaaac aacgcctata cagccactcc
     3961 agcttatgat agtaggttcc aactccaatt cctaaaatat cacaagtacg actgactgaa
     4021 acccttccaa caaccatctt agcgaacatt ggaagaatgg tgttcttttg ttggtgataa
     4081 gtagtggttt cttctctttt tggtagaaca ttagtgaact tcttacacgc cttacattga
     4141 tagcgttgag atttgcccct acttttcccc cttttgtaaa attgcttcgg ctcattgaat
     4201 ggggttgatt cttcttccga gcagccttct ttatgaaatt gataatctgg ctcaacatct
     4261 tggatgctat tgatttctat aaggcgttta atttcttcaa caacagacca attagataaa
     4321 ggggttactg tacagttttg cgatacacct ctgccaatag gattgtcatt gcagtacata
     4381 cctttatgac ctttatcttt ggaactacca gtcattttat atcgacttgg tttccctttt
     4441 acatctttga atttgtgctg ttctttccca aaattacaac aaaacggatt gatgcagtaa
     4501 ttatattgaa tgggatattc agaaccgttc catttaaaag acgttggcaa gaacaacatt
     4561 ttgttatacc ttgttgcaaa tcgttttgga gttaataaat catattgttt agccctatct
     4621 tttaattctt cgtttgaaat cggaatttta acatctacaa tttcatcttc cttcgtggct
     4681 aatcttttaa gcttggtcat tgtctatcaa cccactctat ctttgatacc cattttttca
     4741 acaatccgct tattacttcg cttgttggca atccattcaa taaacttctt atcgtctaaa
     4801 atgttcaata agaattcatc cactgcttcg ctttcagtac gctctgcata ttcagcgtat
     4861 tgcttaacta tcacccttac ttgctctgat atttcccaat ccacagactt agcgttctta
     4921 ttttttggct caagaaattg cataggaaaa tgcacccctt tttgcttagt aaattgtaac
     4981 cattttaaca caccaatttg cattcctaca ataattattt cacgttcgca tttttatata
     5041 cattttaact tacattttgt ggtgtaaatt aacaacaaac gttgatttaa caataaaaaa
     5101 cgagtatcaa attcagttgc aatttgatac tcatttaggt ttacattttc actttttgta
     5161 ctccaaaact gaactactta atatgcaaga cgttgtcttt tttttgtata ttcaattttg
     5221 aaaacatttc cgcaaagttc agacggacct cgatgtgttg atgaataaaa tttattagta
     5281 ataacttcta taaggttatc aatccctata ttactttttg cacttccatg ataaagaggg
     5341 aacagagaac aattctgaaa tcttatgctt tcctcttgtt cgagttccaa tgcttctaat
     5401 gatttaccgg acatatattt ctctaaaagg tcatcgtttc cctctattac cgtatcccat
     5461 tgttcagatt cggtaaagtt cgtcacacac atattaggat acagttctac cttctgtttg
     5521 attacaattt cggcagaaag tttctcttta atatcctgat aaaccgttga taaatcaatt
     5581 ccattttggt caatcttatt gataaaaaag attgtgggaa tccccatttt cctaagtgca
     5641 tgaaataata tacgagtttg tgcttgtacg ccatcttttg cagaaatcag tagaattgcc
     5701 ccatctaaaa ctgataatga acgatatact tctgctaaga aatccatatg tcctggcgtg
     5761 tctatgatgt tcaccttcgt attttcccac tgaaaagagg ttattcctgt ctgaattgta
     5821 attcctctct gacgttctaa aagcgtatta tccgtcctcg ttgtaccttt gtccacgctt
     5881 cctaattctg taatcgctcc actgttatat aataagcttt ctgttaaggt agtttttcct
     5941 gcatcaacat gagctaaaac tccaatatta ataattttca tgtgattttc ctccattcaa
     6001 aaacccaaaa gggcataaaa atcccagtga taaatacttt tatcactggg atttttatgc
     6061 ataaccatag gtatacaaag catacagata ttctctggat actttagaat cacatgataa
     6121 aggtattctt aaactgggta caaaaaacta agccctccta aaaaaggaca tctaattatt
     6181 tgttcccgct atcaaattga cagtttattt aagaatacct tgccgcatat ttattaactc
     6241 cttttaaata gtcacttaaa ta
//
LOCUS       DAYYCY010000386         4165 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.guided.186, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000386 DAYYCY010000000
VERSION     DAYYCY010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4165
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.guided.186"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..304
                     /locus_tag="ACHAZB_002920"
     CDS             <1..304
                     /locus_tag="ACHAZB_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFM8615501.1"
                     /translation="QQEAHYEEKMMPTDEVETTGAPEQPQDELFEEAKALVVEMQTAS
                     ISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKVFIEQEETSETLPDDNDM"
     gene            534..1601
                     /gene="rlmN"
                     /locus_tag="ACHAZB_002921"
     CDS             534..1601
                     /gene="rlmN"
                     /locus_tag="ACHAZB_002921"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFM8615502.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            1591..1797
                     /locus_tag="ACHAZB_002922"
     CDS             1591..1797
                     /locus_tag="ACHAZB_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8615503.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(1794..2897)
                     /locus_tag="ACHAZB_002923"
     CDS             complement(1794..2897)
                     /locus_tag="ACHAZB_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFM8615504.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            3450..3998
                     /gene="aac(6')"
                     /locus_tag="ACHAZB_002924"
     CDS             3450..3998
                     /gene="aac(6')"
                     /locus_tag="ACHAZB_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFM8615505.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         1378 a          707 c          855 g         1225 t
ORIGIN      
        1 tcagcaagaa gctcattatg aagagaaaat gatgccgaca gatgaagttg aaacgacagg
       61 tgctccagaa cagccacaag atgagctgtt tgaagaagca aaagcacttg tcgttgaaat
      121 gcaaacggca agtatttccc ttctccaaag aagatttcgg atcggttaca accgagcagc
      181 acgtttagta gatgaactgg aagctcatgg agttgttggc ccgtcagaag gaagcaagcc
      241 tcggaaagta ttcattgagc aggaagaaac atcagaaacg ttgccggacg ataatgacat
      301 gtaaaacttg tgtgatgaag ggttgaaaaa cgaataacca ataaaaagac aaggcatgat
      361 gataccaaaa gaaaaaatgg tttttttcca tcttttctaa tacggtatga tgtcttgtct
      421 ttttagtttt tgtgagatta aagttctatt cgatttattt gtgaagtatt gcattaagga
      481 aatgaatcat gtatagtatg tagcgaaact ttgagaaaaa gagggaaaat actttggaaa
      541 aacagtccat ttatggcttg acgaatgaag aattaatcaa ttggtttata gaaaacggcg
      601 agaaaaaatt ccgagctgcc caagtttggg aatggttgta tcaaaaacgt gtaagtaatt
      661 tcacagaaat gacgaatcta tcgaaacaat tgatcgaaaa attatcggag cattttatca
      721 tcaatcctct aaaacagatg gtcgtccaag aagcttcgga tggtacagta aaatatttgt
      781 ttgaactccc tgataaaaac atgattgaga ctgtactgat gagacaagaa tatggcttgt
      841 cagtttgtgt gacgacacaa gtaggatgta atatcggctg tacgttttgt gcaagcgggc
      901 tgctcaaaaa gaaccgtgat ctgactgctg gagaaattgt tgcacagatc atgatggtcc
      961 aacattattt tgatgagcgc aatcttggcg aacgcgtttc acatgtcgtt gtaatgggga
     1021 tcggtgagcc atttgataat tacgacaatg taatggactt tttgcatatc atcaatgatg
     1081 ccaaaggatt ggcaattggt gctcggcaca ttacagtttc cactagtgga ttagcacaca
     1141 aaatcaagga attcgctaat aacggcttgc aagtcaatct ggctatttcc ttgcacgcgc
     1201 cgaataatga ggtgcgaaca tcgatcatgc ggatcaaccg aagtttccca attgagaaat
     1261 tgatggaagc tgtggatgag tatttagaga aaacaaaccg tcgaatcacg tttgaatata
     1321 tcatgttgaa tcaagtaaat gatagaccag agcatgcgca acaattagca gatctattaa
     1381 aagataagaa aaaattagca tatgtcaact tgattccata taatccagtc agtgaacatg
     1441 atcaatattc tcgcagtcca aaagctgatg tattgagatt ttatgatgta ttgaagaaaa
     1501 acggcgtaaa ctgtgtgata cgtaaagaac atggtacaga tattgatgca gcttgcggac
     1561 aattgcgaag caagcaaatg aaaaaaacag ttgccaaata atcgtatcct actcgttttt
     1621 tcaattacca taaaaggagc agttttctta attaaggaac tgttcaactt aaataagaga
     1681 aaagattcta tagcgaaagc tataaagctt tttttgacaa gaaaaaccct catcaagcaa
     1741 agaaatgtcc tcaatttgat gacagcctgc ttgatggggg ttttagcaat tatttaactg
     1801 attttcttat atccttgctg gctttcttgt gtagcatgat aagtttctac gtcaagaatg
     1861 gcctgtttaa atttttctcg ggtgatttca taaggcacat gtttaatatc atttgttgcc
     1921 atggctttat ctaagattgc ttctaaatcg gtcgtgacat tgagattcat gtcggttaac
     1981 gatactggaa gttggttctt tttcataaac acatagattt tatcaaacaa atcgaactgc
     2041 tgatccatca tcaataaggt cagtacacca taactgacag atgctccgtg aaggtatccc
     2101 tccatttttt ctagaacagt cgtaccgtaa tagaatgagt gtgccatagc agaattatag
     2161 ttattgttaa ctaaaacaga aaccaatccg gtagtaacaa tgatatccga aacgacttgt
     2221 tcaattgcat atgagggacg atcgtcggca ctatctttca atgcttgcgt accataagtc
     2281 aagagacgat caatgcacat ttttccgatt tggattccta atctatccgt gtagcctaga
     2341 tcatcgccac gagcagaaaa agtagattcg acttctttgg ataaagcatc gccaatacct
     2401 gcccataagt atttatcagg tgcttcggca ataattgctg tatcaatgaa ggtgtgtaca
     2461 ggtggttttg aaggaaagaa gagtccgtcc attacgcctt cttcgttata gatgacacat
     2521 accgcagtga cgggcgaaca gttcgaacca atagtgggaa aggtgaagac tggtttagct
     2581 agtttttcac caatgacttt tacagtatcg caagcacggc caccaccgac tgcaaacagg
     2641 acgtctgctt tttggacact ttcatgctgg atcaactgtt caatatttcc ataagttgaa
     2701 ttacccccgt accagagact ggcgacaacc tctatttcct gtgtcaaagc tacttttatt
     2761 ttgttttctg cctttgaaag tgcagtcttc cctccaatca gcactacttt tgtcccatat
     2821 tctttgacga ttccatttgc tttttcatac gcatctgttc caatggtata atttggtaag
     2881 aaaacacttt gactcatatt catttccttc tttcttcgtt gaaacgggtt aaaattttaa
     2941 agagtgatcg atctgactgc ggctgtagat ggagtagagg tgaacctaag acaggataag
     3001 tgatgaataa ggtggcatga acaccttgaa atcatcaata agtatcctaa aaacgaacaa
     3061 gtacaaaaac attgaaaacg acaataatga atttttgttc ctcttagtat ttctaccgat
     3121 tccctttgct tttttgtaat tatctaaaaa ttctaaaaat taagattatt atatgaaaac
     3181 aggttttatt tgtcaattca taattggttt tttttaaagc aaaagataat ctatatcatg
     3241 atgaacaaac agagtttttt cctccaattg atggctttcc tgatctttcc tattttgaat
     3301 aaagtgtttc tgtataattt ttttatttta taagtaagat aaataatgaa agaatagaga
     3361 tactgattgg tagctaaaga tttttgagcg agaacatttc gttttttcaa aaggactgtt
     3421 agaataagca agaacaaggg ggaagacgta tgataatcag tgaatttgac cgtaataatc
     3481 cagtattgaa agatcagctt tctgatttac tgagactgac ttggccggaa gaatatggag
     3541 acagctcggc agaagaagta gaagaaatga tgaatccaga acgaatcgcg gtagcagcgg
     3601 tagaccaaga tgagttagta ggatttattg gtgcaatccc tcaatacggt atcacaggtt
     3661 gggaattgca tccattagtt gtagaaagct cccgacgaaa gaaccaaata ggtactcgat
     3721 tagtcaatta cttagaaaaa gaagtagctt ccagaggagg aatcacgatt tatttaggta
     3781 cggatgattt agaccatgga acaacgttaa gtcaaacgga cctgtatgag catacatttg
     3841 ataaagtggc ttccatccag aaccttcgtg aacatccgta tgaattctat gaaaaattag
     3901 gttataaaat cgtaggtgtc ttaccaaatg caaatggctg ggacaaaccg gatatttgga
     3961 tggcaaaaac gattattcct cgaccaaatt ctcaataata aaaagaggtt gtgacagaag
     4021 taatcagctt caagaaataa gaaggaattt acgaaaatta cttcttgaat ttttgtgaaa
     4081 ttccaactta tttccgaaga agctactttt ggaacaccgt gtaccagagg ttgtgaaaaa
     4141 gtcgcttagt ttcgagtagt aagaa
//
LOCUS       DAYYCY010000387         3823 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.guided.193, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000387 DAYYCY010000000
VERSION     DAYYCY010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3823
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.guided.193"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            235..699
                     /locus_tag="ACHAZB_002925"
     CDS             235..699
                     /locus_tag="ACHAZB_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM8615506.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            683..1312
                     /locus_tag="ACHAZB_002926"
     CDS             683..1312
                     /locus_tag="ACHAZB_002926"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFM8615507.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            1666..3144
                     /gene="msr(C)"
                     /locus_tag="ACHAZB_002927"
     CDS             1666..3144
                     /gene="msr(C)"
                     /locus_tag="ACHAZB_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFM8615508.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            3191..3637
                     /locus_tag="ACHAZB_002928"
     CDS             3191..3637
                     /locus_tag="ACHAZB_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFM8615509.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
BASE COUNT         1281 a          622 c          791 g         1129 t
ORIGIN      
        1 tctactgcag aagagctatc ttctatagtt tgataagaat caataatctt ggttttagcg
       61 ttgtgctttc tttatcattt tttccgttca ttctacaaaa aatttctttt ctctatactt
      121 ttctattgag agcgtttttt ttattacaca gctgattctt tgactttttt ctttttttat
      181 tctattattg acaagtcaat cgttgacgag tcaattatta gaaaggagga aattatgtct
      241 ttagaagaga ttagtcacct tttatatcaa atcaaattag ccgatcaaaa aatcgtccag
      301 atctttgaag aaaatatcgg tataagtctt actagatacg agatcatgat gagactgaaa
      361 gaagaaaatc cattgctaca aagcagtttg caagaaaagt tgcagattga tcaaggagca
      421 gttactcgtc atctgaaggt attggaagca aatggctatg tttcgcgtca gagaaatcct
      481 cagaacaacc gggaagtgta tgtagcgcta acagaaaaag cccagaagaa aatcaagcat
      541 tgtgaaatgg accataaaga tacaggttcc gtgctatccc cggctttcag cgatgtagaa
      601 atgaaacaat ttctaacact tttagataaa ttgaataccc aactttgcaa tataacaaca
      661 gaaacgaaag aggttaataa taatggaaca aaaaaatgat ttttcaacaa ttatgactgg
      721 tagaagatcc gttcgcgtat atgatgaaac atacaaaatc ccacacgaag aaatgctgga
      781 aatgatcaaa gaagcgacga ctgcaccttc ttctgtcaat atgaaaccat ggcgctttgt
      841 ggtcgctgaa agcgaggaag caaaagaaat cttgcgtcct ttgatccgtt tcaatacgag
      901 acaaaatgat acttcttctg caatggtcat gatttttggc gatatgcaat gttacgaata
      961 tggcgaagaa atctacaacc aagcttacga atcagggaag atgccaaaag aagtcagaga
     1021 tcaacaatta gctgcgatca ttccttatta caagagtttt tcaagagaag aaatgaacga
     1081 tgtagtcaaa gtcgattcta gcttagcggc tatgcaattc atgttagttg cgcgtgatca
     1141 tggctatgaa acaaatccaa ttggtggatt cgaagcagat cagttggcag aggcatttgg
     1201 tttggataaa gaccgttatg ttccagttat tattttatca ataggaaaag cggtagaaga
     1261 aggttacgag tctgttcgtt tagcaccaga aaagatcact atgttcgaat aaaaggtttt
     1321 cagttactat tcagtagcta ttgctttttt tgaatcgtgt gtggtaacct aataaaaaag
     1381 gaggtcggaa aaatgactgc atcgatgaaa ttacgtttcg aacttttgaa taacaactaa
     1441 ataaattcaa aagacatgta taaacaacaa tttcatggat ggagtatgtc cattttttcg
     1501 aacgacagta gaataggtga aagctatgac agatttggtg atttagtcat agttatttcg
     1561 ttcagagtag gaaaagatgt gatgcagttt ttttgcaaac agatttttct gctctttttt
     1621 agtatcgata attgttgttt tacatgaaat aggagtgtaa aacatatgga aaatttagca
     1681 gtaaatataa caaatctaca agtcagcttt ggcaaccagc tagaactatc tattgattct
     1741 cttcgtgtct atcagcaaga tcgtataggg atcataggag aaaatggtgt cggtaaatcg
     1801 actttgctca aactaatagc cggtgaactt tttcccgatc atgggaaaat ccaaacagag
     1861 atcaccttca actacctgcc tcaattaacc tatctcgctg aagcaaagga cctaaatttg
     1921 gaattagcca gtcatttcca gttaagactg gaagaaactt cagagcggaa atggagcgga
     1981 ggggaagaac gaaagatcga gttgatacgt cttctttctt cttatgaaca agggatgctt
     2041 ctagacgagc caacaaccca tctagataga aaaagtattg atcgactgat tgaagagctt
     2101 cgttattatt atggtacgct ggtttttgtt agtcatgatc gctattttct agatgagcta
     2161 gcatcgaaaa tctgggaagt aaaagacgga gaaatccgag agttttcggg gaattatagt
     2221 gcctatctca ctcaaaagga attggagaaa aagactcagc tacgagaagc agagtcgatc
     2281 atgaaagaga aaaaacgatt ggaaaaatcg atccaagaaa agaaaaaaca agcggaaaag
     2341 ttggaaaaag tgtccagtaa aaagaaaaag caacaaatca gactggatcg gttgtcttcc
     2401 tctaaacaaa aagacagtgt acaaaaagcc atccaaaaga atgcgaaaac attagagaga
     2461 agactccaaa aaataggaga aacaaccaag ccgcagcaga tgaaacaaat ccgttttcca
     2521 gtaccaaaat ctcttgaact ccacagccgc tatcccatca tgggacaaaa tgtccaattg
     2581 gaaagaagtg ggagaacatt actggtaaat ggtgattttc agttttcttt aggtaaaaaa
     2641 atcgcgattg tcggtgaaaa tggttcaggt aagacaacct tattagaaca tatccgcaaa
     2701 caaggagaag gaatccttct ctctccgaaa gtaagctttc aagtatatca gcaaaagggt
     2761 tatcaaatga catctgaaga atccatcatt cgttttgtca tgagacaaac agagttttca
     2821 gaatcgcttg tccgtagttt gctgaatcac ttagggtttg ctcaggaaac tttgacgaaa
     2881 ccgttatgta cattaagtgg gggagaagcg acccgtctga cgattgcttt gctttttact
     2941 aagccaagta atgtgttgct gttagatgaa ccgactaatt ttattgatat ggcaacgatc
     3001 gaagctttag agaagctaat gcaaatatat ccgggaacga ttttgtttac ttctcatgat
     3061 tcttactttg tcgagcgtac ggctgatgaa gtttatgaaa taaaagggca gaaaataaaa
     3121 aaagtactta cgagaaattt ttaaatgtgc tacgatgatt ttcgtgacaa atttaaagga
     3181 gtggtacaga ttgcctacaa ataaaaaaga aggaataatt tttacaacaa tgatgtgttt
     3241 tttgatggtc ttggggatga gcgcgtataa tttatggctg catcaagatc ttcatttatc
     3301 aagtttgttg attggtctag tgcctggatt cgtggtggct ttcattttgg atgtcttcat
     3361 catcggtgtg ctggcgaaaa aaattgcctt taaactacca gtaaatccag aaagcaaggt
     3421 ccaaatgatc ttaactattt catgcttgat ggttttagga atggtgacat gtatgtcttt
     3481 attcggtgtg atcatggaac aaggaatacc tgatcaatta ttctctgctt atatgaatgc
     3541 ttggaaaatg aatgtgattg ccgctttacc gttgcagttg atcatcgttg gcccgctttc
     3601 aagaagtgtg ctaaaagcaa ttcaaagtaa tgaatgatgg aacactgtta tttgaaaaaa
     3661 ggccttggca tgctttttgt catagtctct tgaatgcagt tctagaaaaa tagtgatccg
     3721 aacgataggt tttggcctgt cgttcggatc gctttttctt ttccaactgt attgctcttt
     3781 tattgaagat aatttgctct tcaatagata tttctagaaa ggt
//
LOCUS       DAYYCY010000388         3768 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.guided.194, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000388 DAYYCY010000000
VERSION     DAYYCY010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3768
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.guided.194"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            66..1160
                     /gene="vanS"
                     /locus_tag="ACHAZB_002929"
     CDS             66..1160
                     /gene="vanS"
                     /locus_tag="ACHAZB_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000791309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS"
                     /protein_id="HFM8615510.1"
                     /translation="MYIVAIVVVAIVFVLYIRSMIRGKLGDWILSILENKYDLNHLDA
                     MKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKYFDEINTGIDVLIQNEDKQIE
                     LSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVMYLAHDIKTPLTSIIGYLSLL
                     DEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRYNLQTITLTKTHIDLYYMLVQ
                     MTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFNNILKNAAAYSEDNSIIDITA
                     GLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSSDTGGAGLGLAIAKEIIVQHG
                     GQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            1375..2343
                     /locus_tag="ACHAZB_002930"
     CDS             1375..2343
                     /locus_tag="ACHAZB_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFM8615511.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            2336..3367
                     /gene="vanA"
                     /locus_tag="ACHAZB_002931"
     CDS             2336..3367
                     /gene="vanA"
                     /locus_tag="ACHAZB_002931"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFM8615512.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            3373..3738
                     /locus_tag="ACHAZB_002932"
                     /pseudo
     CDS             3373..3738
                     /locus_tag="ACHAZB_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
BASE COUNT         1148 a          715 c          899 g         1006 t
ORIGIN      
        1 gggggttggt tataaaattg aaaaataaaa aaaacgacta atccaaacta gaacgaaaac
       61 tttacatgta tatcgttgca attgttgtgg tagcaattgt attcgtgttg tatattcgtt
      121 caatgatccg agggaaactt ggggattgga tcttaagtat tttggaaaac aaatatgact
      181 taaatcacct ggacgcgatg aaattatatc aatattccat acggaacaat atagatatct
      241 ttatttatgt ggcgattgtc attagtattc ttattctatg tcgcgtcatg ctttcaaaat
      301 tcgcaaaata ctttgacgag ataaataccg gcattgatgt acttattcag aacgaagata
      361 aacaaattga gctttctgcg gaaatggatg ttatggaaca aaagctcaac acattaaaac
      421 ggactctgga aaagcgagag caggatgcaa agctggccga acaaagaaaa aatgacgttg
      481 ttatgtactt ggcgcacgat attaaaacgc cccttacatc cattatcggt tatttgagcc
      541 tgcttgacga ggctccagac atgccggtag atcaaaaggc aaagtatgtg catatcacgt
      601 tggacaaagc gtatcgactc gaacagctaa tcgacgagtt ttttgagatt acacggtata
      661 acctacaaac gataacgcta acaaaaacgc acatagacct atactatatg ctggtgcaga
      721 tgaccgatga attttatcct cagctttccg cacatggaaa acaggcggtt attcacgccc
      781 ccgaggatct gaccgtgtcc ggcgaccctg ataaactcgc gagagtcttt aacaacattt
      841 tgaaaaacgc cgctgcatac agtgaggata acagcatcat tgacattacc gcgggcctct
      901 ccggggatgt ggtgtcaatc gaattcaaga acactggaag catcccaaaa gataagctag
      961 ctgccatatt tgaaaagttc tataggctgg acaatgctcg ttcttccgat acgggtggcg
     1021 cgggacttgg attggcgatt gcaaaagaaa ttattgttca gcatggaggg cagatttacg
     1081 cggaaagcaa tgataactat acgacgttta gggtagagct tccagcgatg ccagacttgg
     1141 ttgataaaag gaggtcctaa gagatgtata taatttttta ggaaaatctc aaggttatct
     1201 ttactttttc ttaggaaatt aacaatttaa tattaagaaa cggctcgttc ttacacggta
     1261 gacttaatac cgtaagaacg agccgttttc gttcttcaga gaaagatttg acaagattac
     1321 cattggcatc cccgttttat ttggtgcctt tcacagaaag ggttggtctt aattatgaat
     1381 aacatcggca ttactgttta tggatgtgag caggatgagg cagatgcatt ccatgctctt
     1441 tcgcctcgct ttggcgttat ggcaacgata attaacgcca acgtgtcgga atccaacgcc
     1501 aaatccgcgc ctttcaatca atgtatcagt gtgggacata aatcagagat ttccgcctct
     1561 attcttcttg cgctgaagag agccggtgtg aaatatattt ctacccgaag catcggctgc
     1621 aatcatatag atacaactgc tgctaagaga atgggcatca ctgtcgacaa tgtggcgtac
     1681 tcgccggata gcgttgccga ttatactatg atgctaattc ttatggcagt acgcaacgta
     1741 aaatcgattg tgcgctctgt ggaaaaacat gatttcaggt tggacagcga ccgtggcaag
     1801 gtactcagcg acatgacagt tggtgtggtg ggaacgggcc agataggcaa agcggttatt
     1861 gagcggctgc gaggatttgg atgtaaagtg ttggcttata gtcgcagccg aagtatagag
     1921 gtaaactatg taccgtttga tgagttgctg caaaatagcg atatcgttac gcttcatgtg
     1981 ccgctcaata cggatacgca ctatattatc agccacgaac aaatacagag aatgaagcaa
     2041 ggagcatttc ttatcaatac tgggcgcggt ccacttgtag atacctatga gttggttaaa
     2101 gcattagaaa acgggaaact gggcggtgcc gcattggatg tattggaagg agaggaagag
     2161 tttttctact ctgattgcac ccaaaaacca attgataatc aatttttact taaacttcaa
     2221 agaatgccta acgtgataat cacaccgcat acggcctatt ataccgagca agcgttgcgt
     2281 gataccgttg aaaaaaccat taaaaactgt ttggattttg aaaggagaca ggagcatgaa
     2341 tagaataaaa gttgcaatac tgtttggggg ttgctcagag gagcatgacg tatcggtaaa
     2401 atctgcaata gagatagccg ctaacattaa taaagaaaaa tacgagccgt tatacattgg
     2461 aattacgaaa tctggtgtat ggaaaatgtg cgaaaaacct tgcgcggaat gggaaaacga
     2521 caattgctat tcagctgtac tctcgccgga taaaaaaatg cacggattac ttgttaaaaa
     2581 gaaccatgaa tatgaaatca accatgttga tgtagcattt tcagctttgc atggcaagtc
     2641 aggtgaagat ggatccatac aaggtctgtt tgaattgtcc ggtatccctt ttgtaggctg
     2701 cgatattcaa agctcagcaa tttgtatgga caaatcgttg acatacatcg ttgcgaaaaa
     2761 tgctgggata gctactcccg ccttttgggt tattaataaa gatgataggc cggtggcagc
     2821 tacgtttacc tatcctgttt ttgttaagcc ggcgcgttca ggctcatcct tcggtgtgaa
     2881 aaaagtcaat agcgcggacg aattggacta cgcaattgaa tcggcaagac aatatgacag
     2941 caaaatctta attgagcagg ctgtttcggg ctgtgaggtc ggttgtgcgg tattgggaaa
     3001 cagtgccgcg ttagttgttg gcgaggtgga ccaaatcagg ctgcagtacg gaatctttcg
     3061 tattcatcag gaagtcgagc cggaaaaagg ctctgaaaac gcagttataa ccgttcccgc
     3121 agacctttca gcagaggagc gaggacggat acaggaaacg gcaaaaaaaa tatataaagc
     3181 gctcggctgt agaggtctag cccgtgtgga tatgttttta caagataacg gccgcattgt
     3241 actgaacgaa gtcaatactc tgcccggttt cacgtcatac agtcgttatc cccgtatgat
     3301 ggccgctgca ggtattgcac ttcccgaact gattgaccgc ttgatcgtat tagcgttaaa
     3361 ggggtgataa gcatggaaat aggatttact tttttagatg aaatagtaca cggtgttcgt
     3421 tgggacgcta aatatgccac ttgggataat ttcaccggaa aaccggttga cggttatgaa
     3481 gtaaatcgca ttgtagggac atacgagttg gctgaatcgc ttttgaaggc aaaagaactg
     3541 gctgctaccc aagggtacgg attgcttcta tgggacggtt accgtcctaa gcgtgctgta
     3601 aactgtttta tgcaatgggc tgcacagccg gaaaataacc tgacaaagga aagttattat
     3661 cccaatattg accgaactga gatgatttca aaaggatacg tggcttcaaa atcaagccat
     3721 agccgcggcg gttctgttgc aaagttttaa ataaagaata aaatccct
//
LOCUS       DAYYCY010000389         3768 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.guided.195, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000389 DAYYCY010000000
VERSION     DAYYCY010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3768
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.guided.195"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..1160
                     /locus_tag="ACHAZB_002933"
     CDS             6..1160
                     /locus_tag="ACHAZB_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-A"
                     /protein_id="HFM8615513.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            1375..2343
                     /locus_tag="ACHAZB_002934"
     CDS             1375..2343
                     /locus_tag="ACHAZB_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFM8615514.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            2336..3367
                     /gene="vanA"
                     /locus_tag="ACHAZB_002935"
     CDS             2336..3367
                     /gene="vanA"
                     /locus_tag="ACHAZB_002935"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFM8615515.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            3373..3738
                     /locus_tag="ACHAZB_002936"
                     /pseudo
     CDS             3373..3738
                     /locus_tag="ACHAZB_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
BASE COUNT         1147 a          715 c          899 g         1007 t
ORIGIN      
        1 gggggttggt tataaaattg aaaaataaaa aaaacgacta ttccaaacta gaacgaaaac
       61 tttacatgta tatcgttgca attgttgtgg tagcaattgt attcgtgttg tatattcgtt
      121 caatgatccg agggaaactt ggggattgga tcttaagtat tttggaaaac aaatatgact
      181 taaatcacct ggacgcgatg aaattatatc aatattccat acggaacaat atagatatct
      241 ttatttatgt ggcgattgtc attagtattc ttattctatg tcgcgtcatg ctttcaaaat
      301 tcgcaaaata ctttgacgag ataaataccg gcattgatgt acttattcag aacgaagata
      361 aacaaattga gctttctgcg gaaatggatg ttatggaaca aaagctcaac acattaaaac
      421 ggactctgga aaagcgagag caggatgcaa agctggccga acaaagaaaa aatgacgttg
      481 ttatgtactt ggcgcacgat attaaaacgc cccttacatc cattatcggt tatttgagcc
      541 tgcttgacga ggctccagac atgccggtag atcaaaaggc aaagtatgtg catatcacgt
      601 tggacaaagc gtatcgactc gaacagctaa tcgacgagtt ttttgagatt acacggtata
      661 acctacaaac gataacgcta acaaaaacgc acatagacct atactatatg ctggtgcaga
      721 tgaccgatga attttatcct cagctttccg cacatggaaa acaggcggtt attcacgccc
      781 ccgaggatct gaccgtgtcc ggcgaccctg ataaactcgc gagagtcttt aacaacattt
      841 tgaaaaacgc cgctgcatac agtgaggata acagcatcat tgacattacc gcgggcctct
      901 ccggggatgt ggtgtcaatc gaattcaaga acactggaag catcccaaaa gataagctag
      961 ctgccatatt tgaaaagttc tataggctgg acaatgctcg ttcttccgat acgggtggcg
     1021 cgggacttgg attggcgatt gcaaaagaaa ttattgttca gcatggaggg cagatttacg
     1081 cggaaagcaa tgataactat acgacgttta gggtagagct tccagcgatg ccagacttgg
     1141 ttgataaaag gaggtcctaa gagatgtata taatttttta ggaaaatctc aaggttatct
     1201 ttactttttc ttaggaaatt aacaatttaa tattaagaaa cggctcgttc ttacacggta
     1261 gacttaatac cgtaagaacg agccgttttc gttcttcaga gaaagatttg acaagattac
     1321 cattggcatc cccgttttat ttggtgcctt tcacagaaag ggttggtctt aattatgaat
     1381 aacatcggca ttactgttta tggatgtgag caggatgagg cagatgcatt ccatgctctt
     1441 tcgcctcgct ttggcgttat ggcaacgata attaacgcca acgtgtcgga atccaacgcc
     1501 aaatccgcgc ctttcaatca atgtatcagt gtgggacata aatcagagat ttccgcctct
     1561 attcttcttg cgctgaagag agccggtgtg aaatatattt ctacccgaag catcggctgc
     1621 aatcatatag atacaactgc tgctaagaga atgggcatca ctgtcgacaa tgtggcgtac
     1681 tcgccggata gcgttgccga ttatactatg atgctaattc ttatggcagt acgcaacgta
     1741 aaatcgattg tgcgctctgt ggaaaaacat gatttcaggt tggacagcga ccgtggcaag
     1801 gtactcagcg acatgacagt tggtgtggtg ggaacgggcc agataggcaa agcggttatt
     1861 gagcggctgc gaggatttgg atgtaaagtg ttggcttata gtcgcagccg aagtatagag
     1921 gtaaactatg taccgtttga tgagttgctg caaaatagcg atatcgttac gcttcatgtg
     1981 ccgctcaata cggatacgca ctatattatc agccacgaac aaatacagag aatgaagcaa
     2041 ggagcatttc ttatcaatac tgggcgcggt ccacttgtag atacctatga gttggttaaa
     2101 gcattagaaa acgggaaact gggcggtgcc gcattggatg tattggaagg agaggaagag
     2161 tttttctact ctgattgcac ccaaaaacca attgataatc aatttttact taaacttcaa
     2221 agaatgccta acgtgataat cacaccgcat acggcctatt ataccgagca agcgttgcgt
     2281 gataccgttg aaaaaaccat taaaaactgt ttggattttg aaaggagaca ggagcatgaa
     2341 tagaataaaa gttgcaatac tgtttggggg ttgctcagag gagcatgacg tatcggtaaa
     2401 atctgcaata gagatagccg ctaacattaa taaagaaaaa tacgagccgt tatacattgg
     2461 aattacgaaa tctggtgtat ggaaaatgtg cgaaaaacct tgcgcggaat gggaaaacga
     2521 caattgctat tcagctgtac tctcgccgga taaaaaaatg cacggattac ttgttaaaaa
     2581 gaaccatgaa tatgaaatca accatgttga tgtagcattt tcagctttgc atggcaagtc
     2641 aggtgaagat ggatccatac aaggtctgtt tgaattgtcc ggtatccctt ttgtaggctg
     2701 cgatattcaa agctcagcaa tttgtatgga caaatcgttg acatacatcg ttgcgaaaaa
     2761 tgctgggata gctactcccg ccttttgggt tattaataaa gatgataggc cggtggcagc
     2821 tacgtttacc tatcctgttt ttgttaagcc ggcgcgttca ggctcatcct tcggtgtgaa
     2881 aaaagtcaat agcgcggacg aattggacta cgcaattgaa tcggcaagac aatatgacag
     2941 caaaatctta attgagcagg ctgtttcggg ctgtgaggtc ggttgtgcgg tattgggaaa
     3001 cagtgccgcg ttagttgttg gcgaggtgga ccaaatcagg ctgcagtacg gaatctttcg
     3061 tattcatcag gaagtcgagc cggaaaaagg ctctgaaaac gcagttataa ccgttcccgc
     3121 agacctttca gcagaggagc gaggacggat acaggaaacg gcaaaaaaaa tatataaagc
     3181 gctcggctgt agaggtctag cccgtgtgga tatgttttta caagataacg gccgcattgt
     3241 actgaacgaa gtcaatactc tgcccggttt cacgtcatac agtcgttatc cccgtatgat
     3301 ggccgctgca ggtattgcac ttcccgaact gattgaccgc ttgatcgtat tagcgttaaa
     3361 ggggtgataa gcatggaaat aggatttact tttttagatg aaatagtaca cggtgttcgt
     3421 tgggacgcta aatatgccac ttgggataat ttcaccggaa aaccggttga cggttatgaa
     3481 gtaaatcgca ttgtagggac atacgagttg gctgaatcgc ttttgaaggc aaaagaactg
     3541 gctgctaccc aagggtacgg attgcttcta tgggacggtt accgtcctaa gcgtgctgta
     3601 aactgtttta tgcaatgggc tgcacagccg gaaaataacc tgacaaagga aagttattat
     3661 cccaatattg accgaactga gatgatttca aaaggatacg tggcttcaaa atcaagccat
     3721 agccgcggcg gttctgttgc aaagttttaa ataaagaata aaatccct
//
LOCUS       DAYYCY010000390         2268 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.guided.239, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000390 DAYYCY010000000
VERSION     DAYYCY010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.guided.239"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..240)
                     /locus_tag="ACHAZB_002937"
     CDS             complement(<1..240)
                     /locus_tag="ACHAZB_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HFM8615516.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFM"
     gene            333..682
                     /locus_tag="ACHAZB_002938"
                     /pseudo
     CDS             333..682
                     /locus_tag="ACHAZB_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(954..1244)
                     /locus_tag="ACHAZB_002939"
     CDS             complement(954..1244)
                     /locus_tag="ACHAZB_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8615517.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1452..2144)
                     /locus_tag="ACHAZB_002940"
     CDS             complement(1452..2144)
                     /locus_tag="ACHAZB_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFM8615518.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQALTDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          645 a          429 c          332 g          862 t
ORIGIN      
        1 cataaagtgt ttagaaaaga acattaagaa tagttctata ataattcttg aactccatgt
       61 cttataatgt tctatcgaag cacccaaata gccaagttca tctaatctac ttgcataaac
      121 tacgtcatcc gctctcatag tcggcatata catattaaag agtaaaaaga taccccaaat
      181 ccctattagt aacaagatca gttctttgtt attcgttatt tttctattat gtttgttcat
      241 ttttatactc cttcttatat ttcctaatag agtataacat agctttaagc attacttaaa
      301 gataacttaa aagttatctt ttaaatactt aattgttaaa taccttattt gatgcaaaag
      361 aaatctacga tagtcaacaa aaaaattttc attatatcca gaaaaatttt tcttagttaa
      421 ggacctatgg attgcagtaa tgcagaattc gtcgaataaa ctgccaaaga catacaatca
      481 tattgctttt aaaattgacg aacaagatat agattcgttt gtctcaaaaa tccaaatgtt
      541 aggtttaact gtcgaaccag gacgttctcg tgtgaaaggc gaggcttctt caatttactt
      601 ttacgactat gataatcacc tcttcgagtt acataccggt actctccagg aacgtttaaa
      661 atcctacaat tccactacct gagactagta atgactagtc tctttttatg gcctctgatc
      721 cccccattga gcgaatacta atagggcttc gaattgatct gttcaatctt taagcaatat
      781 ccctcaaatg agtttggaaa gtttagtgat tcatcacaaa tacttaaata catgtagtta
      841 tatatagatt gaggttcttg tgagcgtatg ctcgtgtggg ttcaaatccc atcgtctgca
      901 tccatctttc caaaacaagc ggctaggaca tttttgtctc agccgcttgt tttctagttt
      961 attctttcat cttgtcggac cttttttctt gggtatagac ttttctcata ttctaccaca
     1021 attttgggtt tccctaacac atgataggat ctcacaacaa attgctttct attattataa
     1081 taacctgtaa cgactaactt catcccttcg tctacttcag ctaggaagtt caaactatgg
     1141 gaagcaatca aacaattgac cccatttaaa ctgaaacgga tcaacggtct ggcactaact
     1201 tgcaaagtac ggatcttatc tactgttcca tattttttct ccataacctt cacctcaata
     1261 ccattatacg aacatacatt ccctttgtaa agaagaaact ctgttttatc tttttgactt
     1321 tttgtctatt acctttctta aaatcatatt ggcacttcct gcgaagatga aggacatgta
     1381 ctaaaaaaaa gaggcagcag tatagattac tgttgcttct ttactaattt aggtttactt
     1441 gttaagaaag attatttacg caaatgttct tcaacaattt gctgagattt atcagctgta
     1501 tcttccactt catcttttgc tttttctaac ttttgaatat attgtttcaa gcttgagttt
     1561 tcagcagttg cttcgtctaa tttcttttgt gtatctgtta actgagtttc tacatttgtt
     1621 ttttcttgtg tcagtgttgc gatttgttga ttcaaaccat ttacagtatt ttgtaattct
     1681 gaaagttttg cttgtgcttc ttgtagtctt gctgtccaat ctgcattcag atgattgatt
     1741 tcatcatttt tttggttgat ctcatctagt ttgacattga tttcattttg tttgctttgt
     1801 agttgtgcat ccttgctgac taattgactt tctaagtttt gtttttctgc tgccgattga
     1861 tttttttggt tttctaattc atttactttt gtttggtatt cttggacatc tgcctttgaa
     1921 tcttgcaatg tctgttcttt ttgtgctagt cgagcggtaa gatctgtaag cgcttgctct
     1981 ttatctgtca atgtatgatc aagctgtgtc agattctcat tgattgtatc aatatctgtc
     2041 tctccacccc acaaaataat ttcatttgcg taccgcgtgc ctgtgtagcc aattagacat
     2101 agaaatgcaa cgacaatccc accagtaata aatttctttt tcatttgcat agctccttaa
     2161 tccaatactt ttacatgcta aaagtaagtg atctcaaaca caatgtcaag cgtttttaaa
     2221 ttacatgtgt aattttaata taaatactat caaatactca tttaatcc
//
LOCUS       DAYYCY010000391         2165 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.guided.246, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000391 DAYYCY010000000
VERSION     DAYYCY010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.guided.246"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            22..1524
                     /gene="eat(A)"
                     /locus_tag="ACHAZB_002941"
     CDS             22..1524
                     /gene="eat(A)"
                     /locus_tag="ACHAZB_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFM8615519.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            1591..2118
                     /locus_tag="ACHAZB_002942"
     CDS             1591..2118
                     /locus_tag="ACHAZB_002942"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFM8615520.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
BASE COUNT          759 a          381 c          474 g          551 t
ORIGIN      
        1 ttttttacga ggtgatggaa aatgtctaaa atcgaaataa aaaatctgac attcggctac
       61 gacagccaag gcacattatt atttgaacaa gcaaatctaa attttgacac acaatggaaa
      121 ctaggactta tcggacgaaa cggtcgagga aagacaactt tactgaatat tctacaaaac
      181 aaactacctt atcaagggca agtaatccat cagcaagaat ttgcctattt cccgcaacag
      241 acaaaagata aagaacgttt aacctattac gtgttaaatg atattacgga ttttgagata
      301 tgggaaatcg aaagagagct ccaattgatg caaacagatc ctgaaatctt atggagagaa
      361 ttcagcacac tatcgggggg agagaagaca aaagtcctac tggcactttt atttgtggat
      421 gacactcatt tcccgttaat cgatgaacca acgaatcatt tggatatctc tggtagaaaa
      481 caagtagcgg cttatttgaa aaagaaaaaa caaggcttca tcgtggtcag ccatgaccgg
      541 ggatttatcg atgaagtagt ggaccatgtt ttagcaatcg aaaaaagtca actggaactt
      601 tatcaaggga atttctctat ctatgaagaa cagaaaaaac ttcgtgatga atttgaaatg
      661 gctcaaaatg aaaaattgaa aaaagaagtc agtaggctaa agaaaacagc agctgaaaaa
      721 gccgaatggt ctcgttcccg agaaggagat aaaacaaaga aacaagtcgg attcatcgat
      781 actgaatcta gacgagtgaa taaaggagca gtgggtgctg atgctgcacg gacgatgaaa
      841 cgatccaaag caatcgtgaa tcggatggag acccagatca gcgagaaaga aaaactatta
      901 aaagatatcg aatatatcga ttctttgacg atgaatagcc aagcgtctca ccataagcga
      961 cttttaagcg tagaagatct tcaattaggg tatgaaaatc tgttattcga gccaattcat
     1021 tttacaatcg agcctcatca gcgggtggcg atttcaggtc ctaacggtgc aggaaagtca
     1081 tccattatcc attatcttct gggggcattc aacggcaagg ttataggaga aaaataccag
     1141 ccaaaacatc tgagcattag ttatgcaagc caaaattatg aagacaatcg aggaacgttg
     1201 gcggaatttg cagagaaaaa ccaagtagac taccaagcat ttttgaacaa cctccgaaag
     1261 cttgggatgg aaagagatgt ttttcataac aagatcgagc agatgagtat gggccaacgg
     1321 aaaaaagtgg aattggctaa atctttatca cagccagctg aactatatac atgggatgaa
     1381 ccattgaatt atttggatgt cttcaatcaa gaacaattag aacaactgat cttgaacgtg
     1441 aaacctgcca tgttactagt ggaacatgat caaaccttcc tggataaagt atctactgag
     1501 attatttctc ttgagagaat ctaacccttg taatctcaag aacaacaagg tatggttaag
     1561 gggaactttg ccaacgaagg agagaatcac atgctttatg taacgagaca cggagaaaca
     1621 acttggaacg cacaaggatt agtttgcgga cgggcagatg tgccgctgac tgaaaaagga
     1681 cagatgcagg cacagaaact agcagaaaag gtagtagatt tgcctgttcc aatcacgaaa
     1741 atcatccatt cccctcttca acgagcaaga gatacagcac aagcagttgc tgatcgtctc
     1801 agcttgccgc tgaccgttga tgaacgattg gtagaaatgg attttgggga ttatgatgga
     1861 ctgccaagca aagatgagaa ctttcaaaaa gcacgtttag cgttcgctgt acgattccca
     1921 aatggggagt cagtcttaga tgtgtatgca cggatcgttc ctttattgaa agaatgcatc
     1981 gaagacgaag aaaatgtcta tctgttagtt tgccataatg ccttgataag agtgatcaat
     2041 gcttattttc atccgatgcc taatgagggg ttctttacat tcatggtaga taatacagaa
     2101 ttggtttctt atgagtaatt atgtttttgt gttatttaaa taaaatattt caaggcactg
     2161 ctttt
//
LOCUS       DAYYCY010000392         1141 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.guided.305, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000392 DAYYCY010000000
VERSION     DAYYCY010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1141
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.guided.305"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            172..954
                     /locus_tag="ACHAZB_002943"
     CDS             172..954
                     /locus_tag="ACHAZB_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HFM8615521.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
BASE COUNT          403 a          156 c          227 g          355 t
ORIGIN      
        1 ctgtataata aagaataatt attaatctgt agacaaattg tgaaaggatg tacttaaacg
       61 ctaacggtca gctttattga acagtcattt aggtatatgt ccaatctagg gtaagtaaat
      121 tgagtatcaa tataaacttt atatgaacat aatcagttac gaggtgaaat catgagcaat
      181 ttgattaacg gaaaaatacc aaatcaagcg attcaaacat taaaaatcgt aaaagattta
      241 tttggaagtt caatagttgg agtatatcta tttggttcag cagtaaatgg tggtttacgc
      301 attaacagcg atgtagatgt tctagtcgtc gtgaatcata gtttacctca attaactcga
      361 aaaaaactaa cagaaagact aatgactata tcaggaaaga ttggaaatac ggattctgtt
      421 agaccacttg aagttacggt tataaatagg agtgaagttg tcccttggca atatcctcca
      481 aaaagagaat ttatatacgg tgagtggctc aggggtgaat ttgagaatgg acaaattcag
      541 gaaccaagct atgatcctga tttggctatt gttttagcac aagcaagaaa gaatagtatt
      601 tctctatttg gtcctgattc ttcaagtata cttgtctccg tacctttgac agatattcga
      661 agagcaatta aggattcttt gccagaacta attgagggga taaaaggtga tgagcgtaat
      721 gtaattttaa ccctagctcg aatgtggcaa acagtgacta ctggtgaaat tacctcgaaa
      781 gatgtcgctg cagaatgggc tatacctctt ttacctaaag agcatgtaac tttactggat
      841 atagctagaa aaggctatcg gggagagtgt gatgataagt gggaaggact atattcaaag
      901 gtgaaagcac tcgttaagta tatgaaaaat tctatagaaa cttctctcaa ttaggctaat
      961 tttattgcaa taacaggtgc ttacttttaa aactactgat ttattgataa atattgaaca
     1021 atttttggga agaataaagc gtcctcttgt gaaattagag aacgctttat tactttaatt
     1081 tagtgaaaca atttgtaact attgaaaata gaaagaaatt gttccttcga tagtttatta
     1141 a
//
LOCUS       DAYYCY010000393          881 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.guided.338, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000393 DAYYCY010000000
VERSION     DAYYCY010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..881
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.guided.338"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..810
                     /locus_tag="ACHAZB_002944"
                     /note="aph(2'')-Ia"
     CDS             <1..810
                     /locus_tag="ACHAZB_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /protein_id="HFM8615522.1"
                     /translation="GYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETN
                     VKIPNIEYSYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGL
                     DYTDISECTIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCL
                     CHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRM
                     YGNIDIEKAKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          378 a           62 c          143 g          298 t
ORIGIN      
        1 ggttatgata gtgtggcata tttagttaat aatgaataca tttttaaaac aaaatttagt
       61 actaataaga aaaaaggtta tgcaaaagaa aaagcaatat ataatttttt aaatacaaat
      121 ttagaaacta atgtaaaaat tcctaatatt gaatattcgt atattagtga tgaattatct
      181 atactaggtt ataaagaaat taaaggaact tttttaacac cagaaattta ttctactatg
      241 tcagaagaag aacaaaattt gttaaaacga gatattgcca gttttttaag acaaatgcac
      301 ggtttagatt atacagatat tagtgaatgt actattgata ataaacaaaa tgtattagaa
      361 gagtatatat tgttgcgtga aactatttat aatgatttaa ctgatataga aaaagattat
      421 atagaaagtt ttatggaaag actaaatgca acaacagttt ttgagggtaa aaagtgttta
      481 tgccataatg attttagttg taatcatcta ttgttagatg gcaataatag attaactgga
      541 ataattgatt ttggagattc tggaattata gatgaatatt gtgattttat atacttactt
      601 gaagatagtg aagaagaaat aggaacaaat tttggagaag atatattaag aatgtatgga
      661 aatatagata ttgagaaagc aaaagaatat caagatatag ttgaagaata ttatcctatt
      721 gaaactattg tttatggaat taaaaatatt aaacaggaat ttatcgaaaa tggtagaaaa
      781 gaaatttata aaaggactta taaagattga ttatataata tatgaaaagc tattataaaa
      841 gacggttctg ttgcaaagtt ttaaatctac tatcaaataa g
//
LOCUS       DAYYCY010000394          743 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.guided.346, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000394 DAYYCY010000000
VERSION     DAYYCY010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..743
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.guided.346"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            60..>743
                     /gene="erm(A)"
                     /locus_tag="ACHAZB_002945"
     CDS             60..>743
                     /gene="erm(A)"
                     /locus_tag="ACHAZB_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HFM8615523.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLS"
BASE COUNT          287 a          117 c          128 g          211 t
ORIGIN      
        1 aattggttat aatgaacgct taatgtcagt tcattataac cagtaaggag aaggttataa
       61 tgaaccagaa aaaccctaaa gacacgcaaa attttattac ttctaaaaag catgtaaaag
      121 aaatattgaa tcacacgaat atcagtaaac aagacaacgt aatagaaatc ggatcaggaa
      181 aaggacattt taccaaagag ctagtcaaaa tgagtcgatc agttactgct atagaaattg
      241 atggaggctt atgtcaagtg actaaagaag cggtaaaccc ctctgagaat ataaaagtga
      301 ttcaaacgga tattctaaaa ttttccttcc caaaacatat aaactataag atatatggta
      361 atattcctta taacatcagt acggatattg tcaaaagaat tacctttgaa agtcaggcta
      421 aatatagcta tcttatcgtt gagaagggat ttgcgaaaag attgcaaaat ctgcaacgag
      481 ctttgggttt actattaatg gtggagatgg atataaaaat gctcaaaaaa gtaccaccac
      541 tatattttca tcctaagcca agtgtagact ctgtattgat tgttcttgaa cgacatcaac
      601 cattgatttc aaagaaggac tacaaaaagt atcgatcttt tgtttataag tgggtaaacc
      661 gtgaatatcg tgttcttttc actaaaaacc aattccgaca ggctttgaag catgcaaatg
      721 tcactaatat taataaacta tcg
//
LOCUS       DAYYCY010000395          577 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5365
            SAMEA115707670-rid22593643.guided.359, whole genome shotgun
            sequence.
ACCESSION   DAYYCY010000395 DAYYCY010000000
VERSION     DAYYCY010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707670
            Sequence Read Archive: ERR13197398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290136.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290136.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:47:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,946
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 28 of 79
            Pseudo Genes (incomplete)         :: 41 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 10 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707670-rid22593643.guided.359"
                     /isolate="VRE5365"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>577
                     /locus_tag="ACHAZB_002946"
     CDS             <1..>577
                     /locus_tag="ACHAZB_002946"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8615524.1"
                     /translation="RDFILYLKLCNRTESLKKHYTEPWEDEVFRVIIEYNNVPIGYGQ
                     IYKMYDELYTDYHYPKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANA
                     VILDPHKNNPRAIRAYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMK
                     YLIEHYFDNFKVDSIEIIGSGYDSVAYLVNNE"
BASE COUNT          231 a           53 c          105 g          188 t
ORIGIN      
        1 agggatttta ttctttattt aaaactttgc aacagaaccg aatcattaaa aaaacattat
       61 acagagcctt gggaagatga agtttttaga gtaattattg aatataacaa tgttcctatt
      121 ggatatggac aaatatataa aatgtatgat gagttatata ctgattatca ttatccaaaa
      181 actgatgaga tagtctatgg tatggatcaa tttataggag agccaaatta ttggagtaaa
      241 ggaattggta caagatatat taaattgatt tttgaatttt tgaaaaaaga aagaaatgct
      301 aatgcagtta ttttagaccc tcataaaaat aatccaagag caataagggc ataccaaaaa
      361 tctggtttta gaattattga agatttgcca gaacatgaat tacacgaggg caaaaaagaa
      421 gattgttatt taatggaata tagatatgat gataatgcca caaatgttaa ggcaatgaaa
      481 tatttaattg agcattactt tgataatttc aaagtagata gtattgaaat aatcggtagt
      541 ggttatgata gtgtggcata tttagttaat aatgaat
//
